28 lines
879 B
Plaintext
28 lines
879 B
Plaintext
Frog: A Utility to Relabel Bases in ABI 373A Files
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Simon Dear
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(sd@mrc-lmb.cam.ac.uk)
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20 November 1992
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Correcting ABI 373A traces that have had their bases labelled
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incorrectly is not a simple matter. The program "frog" is a utility to
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swap bases around.
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To use frog you will need to know the existing base lane order, and
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the correct base order. For example, the base order may currently be
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CAGT but examination of the sequence (eg a known cloning site) may
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reveal that it should gave been CATG. With this information, simply
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run frog as follows:
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frog old new [ABI_files...]
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where "old" is the current (incorrect) lane ordering and "new" is the
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correct lane ordering.
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In the previous example we discovered that the T and G lanes have been
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inadvertantly switched. We could correct the file (trace.abi, say) by
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typing:
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frog CAGT CATG trace.abi
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