706 lines
19 KiB
Text
706 lines
19 KiB
Text
macro=;
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log %7.tnt.log;
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/*Arguments*/
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if ( (argnumber == 0) || (argnumber >7) )
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silent -console;
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quote
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/----------------------------------------------------\
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| GUOYI TNT SCRIPT 2022-2023 MIT |
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| You need to give your filename |
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| shell> tnt run guoyi.tnt filename, (Linux & Mac) |
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| shell> tnt run guoyi.run filename(semicolon) (Win) |
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| ============================== |
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| === Parameters Details === |
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| ============================== |
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| filename type weight K cons resamp prefix |
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| - type should be 32, dna, prot, num |
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| num=number, dna=DNA, prot=protein |
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| 32=max number allowed (default) |
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| - weight should be iw, ew, eiw |
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| iw=implied weight, ew=equal weight |
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| eiw=extended implied weight (default) |
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| N.B. K of ew must followed 0 (=NA) |
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| - K is 12 (default) following Goloboff |
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| et al. 2017 (Cladistics 34: 407–437) |
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| it must more than 0 |
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| - cons should be str, mjr, hlf |
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| mjr=majority rule, hlf=half |
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| str=strict (default) |
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| - resamp should be sum of what you want |
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| jak=1, boot=2, sym=4 relative-bremer |
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| (rbr)=0.1, bremer(br)=0.2 |
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| i.e rbr+br+jak+boot+sym=7.3 (default) |
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| - prefix can be empty, or a string |
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| default is empty |
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\----------------------------------------------------/;
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proc/;
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end
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/*Basic settings*/
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taxname+1000;
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taxname=;
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mxram 10240;
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if(argnumber>=2)
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nstates %2;
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else
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nstates 32;
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end
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nstates NOGAPS;
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/*Set K*/
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if(argnumber>2 )
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if (eqstring [ %3 ew ])
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quote Equal weighting is used;
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if ((argnumber>=4) && (%4 != 0))
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errmsg equal weighting %4 should be fullfilled with 0;
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end
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else
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if ((eqstring [ %3 iw ]) || (eqstring [ %3 eiw ]))
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if (argnumber>=4)
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piwe=%4;
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else
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piwe=12;
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end
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else
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errmsg %3 must be ew iw or eiw;
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end end
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else
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piwe=12;
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end
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/*Reopen tnt*/
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procedure %1;
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hold 10000;
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/*Report what will be done*/
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quote
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/-----------------------------------------------\;
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if(argnumber>2)
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if (eqstring [ %3 iw ])
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if (argnumber>3)
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quote
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| Implied weighting will be used, K is %4. |;
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else
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quote
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| Implied weighting will be used, K is 12. |;
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end
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else
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if (eqstring [ %3 eiw ])
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if (argnumber>3)
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quote
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| Ex-implied weighting will be used, K is %4. |;
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else
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quote
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| Ex-implied weighting will be used, K is 12. |;
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end
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else
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if (eqstring [ %3 ew ])
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quote
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| Equal weighting will be used. |;
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else
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errmsg %3 must be ew iw or eiw;
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end end end
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else
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quote
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| Ex-implied weighting will be used, K is 12. |;
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end
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if (ntax<=25)
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quote
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| Implicit enumeration will be performed. |;
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else
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if ((ntax>25) && (ntax < 75))
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quote
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| TBR Mult will be performed. |;
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else
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if (ntax>=75)
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quote
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| Xmult will be performed. |;
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end end end
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if (argnumber<=5)
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quote
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| Strict consensus will be used. |;
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else
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if (( %6 != 0.1 )||( %6 != 0.2 )||( %6 != 0.3 )||( %6!=1.1 )||( %6 !=1.2 )||( %6 !=1.3 )||( %6 != 2.1 )||( %6 != 2.2 )||( %6 != 2.3 )||( %6 != 3.1 )||( %6 != 3.2 )||( %6 != 3.3 )||( %6 != 4.1 )||( %6 != 4.2 )||( %6 != 4.3 )||( %6 != 5.1 )||( %6 != 5.2 )||( %6 != 5.3 )||( %6 != 6.1 )||( %6 != 6.2 )||( %6 != 6.3 )||( %6 != 7.1 )||( %6 != 7.2 )||( %6 != 7.3 ))
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if (!(eqstring [ %5 str ]))
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errmsg Bremer support or relative bremer support must use strict consensus;
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end
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quote
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| Strict consensus will be used. |;
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else
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if (eqstring [ %5 mjr ])
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quote
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| Majority-rule consensus will be used. |;
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else
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if (eqstring [ %5 hlf ])
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quote
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| Half strict consensus will be used. |;
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else
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if(eqstring [ %5 str ])
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quote
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| Strict consensus will be used. |;
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else
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errmsg %5 must be mjr hlf or str;
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end end end
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end end
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if (argnumber>=6)
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if ( (%6>0) && (%6<1) )
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if (%6==0.1 || %6==0.3)
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quote
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| relative bremer support |;
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else
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if (%6==0.2 || %6==0.3)
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quote
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| bremer support |;
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end end
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else
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if ((%6>=1) && (%6<2))
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quote
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| Jackknifing |;
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if (%6==1.1 || %6==1.3)
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quote
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| relative bremer support |;
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else
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if (%6==1.2 || %6==1.3)
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quote
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| bremer support |;
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end end
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else
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if ((%6>=2) && (%6<3))
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quote
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| Bootstrap |;
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if (%6==2.1 || %6==2.3)
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quote
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| relative bremer support |;
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else
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if (%6==2.2 || %6==2.3)
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quote
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| bremer support |;
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end end
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else
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if ((%6>=3) && (%6<4))
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quote
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| Jackknifing and bootstrap |;
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if (%6==3.1 || %6==3.3)
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quote
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| relative bremer support |;
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else
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if (%6==3.2 || %6==3.3)
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quote
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| bremer support |;
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end end
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else
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if ((%6>=4) && (%6<5))
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quote
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| Symmetric resampling |;
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if (%6==4.1 || %6==4.3)
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quote
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| relative bremer support |;
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else
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if (%6==4.2 || %6==4.3)
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quote
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| bremer support |;
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end end
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else
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if ((%6>=5) && (%6<6))
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quote
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| Jackknifing and symmetric resampling |;
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if (%6==5.1 || %6==5.3)
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quote
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| relative bremer support |;
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else
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if (%6==5.2 || %6==5.3)
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quote
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| bremer support |;
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end end
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else
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if ((%6>=6) && (%6<7))
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quote
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| Bootstrap and symmetric resampling |;
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if (%6==6.1 || %6==6.3)
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quote
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| relative bremer support |;
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else
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if (%6==6.2 || %6==6.3)
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quote
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| bremer support |;
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end end
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else
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if ((%6>=7) && (%6<8))
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quote
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| Jackknifing, bootstrap and symmetric |
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| resampling |;
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if (%6==7.1 || %6==7.3)
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quote
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| relative bremer support |;
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else
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if (%6==7.2 || %6==7.3)
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quote
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| bremer support |;
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end end
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else
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errmsg Mistake %6 value;
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end end end end end end end end
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end
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if (argnumber<6)
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quote
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| relative bremer support, bremer support |
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| jackknifing, bootstrap, symmetric |
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| resampling |;
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end
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quote
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| will be shown on the resample.svg. |
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| Apomorphic characters mapping will be shown |
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| on the apo.svg and saved to apo*.tre. |
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| TL, CI and RI will be calculated finally. |
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\------------------------------------------------/;
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/*Implied weighting settings*/
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if(argnumber>2)
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if (eqstring [ %3 iw ])
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piwe&;
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else
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if (eqstring [ %3 eiw ])
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xpiwe(*;
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log %7.eiw.log;
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piwe&;
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log/;
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log + %7.tnt.log;
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else
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if (eqstring [ %3 ew ])
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else
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errmsg %3 must be ew iw or eiw;
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end end end
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else
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xpiwe(*;
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log %7.eiw.log;
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piwe&;
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log/;
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log+ %7.tnt.log;
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end
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/*Search trees*/
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if(argnumber>2)
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if(eqstring [ %3 ew ] || eqstring [ %3 iw ] )
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if (ntax<=25)
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ienum;
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else
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if (ntax<=74)
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mult=replic 1000 tbr hold 1000;
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bbreak=tbr fill;
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else
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if (ntax>74)
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sect: slack 40;
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xmult=hit 50 replications 20 drift 10 ratchet 10 fuse 10 hold 1 keepall;
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end end
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end
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else
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if (ntax<=74)
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mult=replic 1000 tbr hold 1000;
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bbreak=tbr fill;
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else
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sect: slack 40;
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xmult=hit 50 replications 20 drift 20 ratchet 10 fuse 10 hold 1 keepall;
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end
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end
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else
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mult=replic 1000 tbr hold 1000;
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bbreak=tbr fill;
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if (ntax>74)
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sect: slack 1000;
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xmult=hit 50 replications 20 drift 10 ratchet 10 fuse 10 hold 1 keepall;
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end
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end
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/*Export trees*/
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export= %7.trees.tre;
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taxname-;
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export= %7.trees_no.tre;
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taxname=;
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tsave = %7.trees.ctf;
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tsave *= %7.trees.tnt.tre;
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/*Get npars number*/
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var: npars;
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set npars ntrees;
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/*Get rbr/br/jak/boot/sym support and get consensus tree*/
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ttags=;
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ttags]; /*in one line*/
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/* jak=1, boot=2, sym=4, */
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/* jak+boot=3, */
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/* jak+sym=5, boot+sym=6, */
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/* jak+boot+sym=7, */
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/* bremer=0.1, relative-bremer=0.2 */
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/* bremer+relative-bremer=0.3 */
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if (argnumber<=5)
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/*bremer search*/
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sub 1; hold +1000; bbreak=fill;
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sub 3; hold +3000; bbreak=fill;
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sub 5; hold +5000; bbreak=fill;
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sub 7; hold +7000; bbreak=fill;
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/*adjust to zero following Goloboff*/
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sub 0;
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/*get the consensus tree number*/
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var: nsubpars;
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set nsubpars ntrees;
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/*save strict consensus tree for bremer*/
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hold+1;
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bs * 0.'nsubpars';
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/*calculate relative bremer support*/
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bs [ 0.'nsubpars' ;
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/*set the br value float*/
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macfloat 2;
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/*calculate bremer support*/
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bsupport 0.'nsubpars';
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/*choose final tree*/
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tchoose/;
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/*jakknifing*/
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resample jak replications 1000 from 0; /*from 0 will orphan other trees*/
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/*bootstrap*/
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resample boot replications 1000 from 0;
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/*symmetric resampling*/
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resample sym replications 1000 from 0;
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else
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if(( %6 == 0.1 )||( %6 == 0.2 )||( %6 == 0.3 )||( %6==1.1 )||( %6 ==1.2 )||( %6 ==1.3 )||( %6 == 2.1 )||( %6 == 2.2 )||( %6 == 2.3 )||( %6 == 3.1 )||( %6 == 3.2 )||( %6 == 3.3 )||( %6 == 4.1 )||( %6 == 4.2 )||( %6 == 4.3 )||( %6 == 5.1 )||( %6 == 5.2 )||( %6 == 5.3 )||( %6 == 6.1 )||( %6 == 6.2 )||( %6 == 6.3 )||( %6 == 7.1 )||( %6 == 7.2 )||( %6 == 7.3 )) /*bremer support related can only use strict consensus*/
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/*bremer search*/
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sub 1; hold +1000; bbreak=fill;
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sub 3; hold +3000; bbreak=fill;
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sub 5; hold +5000; bbreak=fill;
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sub 7; hold +7000; bbreak=fill;
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/*adjust to zero following Goloboff*/
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sub 0;
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/*get the consensus tree number*/
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var: nsubpars;
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set nsubpars ntrees;
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/*save strict consensus tree for bremer*/
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hold+1;
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bs * 0.'nsubpars';
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if(( %6 == 0.1 )||( %6 == 0.3 )||( %6==1.1 )||( %6 ==1.3 )||( %6 == 2.1 )||( %6 == 2.3 )||( %6 == 3.1 )||( %6 == 3.3 )||( %6 == 4.1 )||( %6 == 4.3 )||( %6 == 5.1 )||( %6 == 5.3 )||( %6 == 6.1 )||( %6 == 6.3 )||( %6 == 7.1 )||( %6 == 7.3 ))
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/*calculate relative bremer support*/
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bs [ 0.'nsubpars';
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end
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if(( %6 == 0.2 )||( %6 == 0.3 )||( %6==1.2 )||( %6 ==1.3 )||( %6 == 2.2 )||( %6 == 2.3 )||( %6 == 3.2 )||( %6 == 3.3 )||( %6 == 4.2 )||( %6 == 4.3 )||( %6 == 5.2 )||( %6 == 5.3 )||( %6 == 6.2 )||( %6 == 6.3 )||( %6 == 7.2 )||( %6 == 7.3 ))
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/*set the br value float*/
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macfloat 2;
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/*calculate bremer support*/
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bsupport 0.'nsubpars';
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end
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/*choose final tree*/
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tchoose/;
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/*jackknifing*/
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if((%6 == 1.1) || (%6 == 1.2) || (%6 == 1.3) || (%6 == 3.1) || (%6 == 3.2) || (%6 == 3.3) || (%6 == 5.1) || (%6 == 5.2) || (%6 == 5.3) || (%6 == 7.1) || (%6 == 7.2) || (%6 == 7.3))
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resample jak replications 1000 from 0;
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end
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/*bootstrap*/
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if((%6 == 2.1) || (%6 == 2.2) || (%6 == 2.3) || (%6 == 3.1) || (%6 == 3.2) || (%6 == 3.3) || (%6 == 6.1) || (%6 == 6.2) || (%6 == 6.3) || (%6 == 7.1) || (%6 == 7.2) || (%6 == 7.3))
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resample boot replications 1000 from 0;
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end
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/*symmetric resampling*/
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if((%6 == 4.1) || (%6 == 4.2) || (%6 == 4.3) || (%6 == 5.1) || (%6 == 5.2) || (%6 == 5.3) || (%6 == 6.1) || (%6 == 6.2) || (%6 == 6.3) || (%6 == 7.1) || (%6 == 7.2) || (%6 == 7.3))
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resample sym replications 1000 from 0;
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end
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else /*non-bremer related should be consensus firstly*/
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if (eqstring [ %5 str ])
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nelsen * 0.'npars';
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else
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if (eqstring [ %5 mjr ])
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majority * 0.'npars';
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else
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if (eqstring [ %5 hlf ])
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comcomp * 0.'npars';
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else
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errmsg %5 must be mjr hlf or str;
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end end end
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/*choose the final tree: consenus tree*/
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tchoose/;
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/*jackkinfing*/
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if((%6==1) || (%6==3) || (%6==5) || (%6==7))
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resample jak replications 1000 from 0;
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end
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/*bootstrap*/
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if((%6==2) || (%6==3) || (%6==6) || (%6==7))
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resample boot replications 1000 from 0;
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end
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/*symmetric resampling*/
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if((%6==4) || (%6==5) || (%6==6) || (%6==7))
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resample sym replications 1000 from 0;
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end
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end
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/*Tree vault store*/
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hold /+0;
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tvault >/;
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end
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/*Export consensus tree with supports*/
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ttags & %7.resample.svg thickness 7 italics fontsize 15;
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log %7.resample.log;
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quote /-------------resample tags start---------------\;
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ttags/;
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quote \-------------resample tags stop----------------/;
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log/;
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log + %7.tnt.log;
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export < %7.resample.tre;
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taxname-;
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export - %7.resample_no.tre;
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ttags-;
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taxname=;
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/*Export consensus tree*/
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tchoose /;
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export - %7.original.tre;
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taxname-;
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export - %7.original_no.tre;
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tsave = %7.original.ctf;
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tsave *= %7.original.tnt.tre;
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/*Apomorphic characters*/
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export = %7.winclada.tre;
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taxname =;
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ttags=;
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apo >0;
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log %7.apo.log;
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quote /-----------apomorphy tags start --------------\;
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ttags/;
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quote \-----------apomorphy tags stop ---------------/;
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log/;
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log + %7.tnt.log;
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ttags & %7.apo.svg thickness 7 italics fontsize 15;
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export < %7.apo.tre;
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taxname-;
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export - %7.apo_no.tre;
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ttags-;
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/*Character Analysis*/
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log %7.homo.log;
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chomo;
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cscores;
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log/;
|
||
log + %7.tnt.log;
|
||
|
||
/*Caulculate TL/CI/RI score*/
|
||
report-;
|
||
var: themin themax CI RI TL ;
|
||
set themin minsteps;
|
||
set themax maxsteps;
|
||
set TL length[0];
|
||
set CI 'themin'/'TL'; /*CI=1 means no homoplasy*/
|
||
set RI ('themax'-'TL')/('themax'-'themin'); /*RI=1 character fits perfetcly*/
|
||
report=;
|
||
|
||
/*Report CI/RI/TL */
|
||
log %7.report.log;
|
||
macfloat 3;
|
||
quote Consistency Index (CI) is 'CI';
|
||
quote Retention Index (RI) is 'RI';
|
||
quote Tree Length (TL) is 'TL';
|
||
log/;
|
||
log + %7.tnt.log;
|
||
|
||
/*Report*/
|
||
quote
|
||
/----------------------------------------------\
|
||
| The analysis has been finished. |
|
||
| The file `tnt.log` contains |
|
||
| K, TL, CI and RI |
|
||
| The file `trees*.tre` contain |
|
||
| trees found by mult and xmult |
|
||
| The file `original*.tre` contain |
|
||
| strict consensus tree without label |
|
||
| The file `resample*.tre` contain |
|
||
| consensus tree with support |
|
||
| The file `apo*.tre` contain |
|
||
| tree with apomorphic character |
|
||
| The file `*_no.tre` contain |
|
||
| tree with `taxname-` |
|
||
| The file `*.ctf` tree file is |
|
||
| only readable for TNT |
|
||
| The file `*.tnt.tre` contain |
|
||
| is the ctf file with taxname |
|
||
| The file `resample.svg` contain |;
|
||
|
||
|
||
if (argnumber<=5)
|
||
quote
|
||
| strict consensus tree with |;
|
||
else
|
||
if ( (argnumber >=6) && (( %6 == 0.1 )||( %6 == 0.2 )||( %6 == 0.3 )||( %6==1.1 )||( %6 ==1.2 )||( %6 ==1.3 )||( %6 == 2.1 )||( %6 == 2.2 )||( %6 == 2.3 )||( %6 == 3.1 )||( %6 == 3.2 )||( %6 == 3.3 )||( %6 == 4.1 )||( %6 == 4.2 )||( %6 == 4.3 )||( %6 == 5.1 )||( %6 == 5.2 )||( %6 == 5.3 )||( %6 == 6.1 )||( %6 == 6.2 )||( %6 == 6.3 )||( %6 == 7.1 )||( %6 == 7.2 )||( %6 == 7.3 )) )
|
||
quote
|
||
| strict consensus tree with |;
|
||
else
|
||
if (eqstring [ %5 mjr ])
|
||
quote
|
||
| majority-rule consensus tree with |;
|
||
else
|
||
if (eqstring [ %5 hlf ])
|
||
quote
|
||
| half strict consensus tree with |;
|
||
else
|
||
if(eqstring [ %5 str ])
|
||
quote
|
||
| strict consensus tree with |;
|
||
else
|
||
errmsg %5 must be mjr hlf or str;
|
||
errmsg Bremer support or relative bremer support must use strict consensus;
|
||
end end end
|
||
end end
|
||
|
||
if (argnumber>=6)
|
||
if ( (%6>0) && (%6<1) )
|
||
if (%6==0.1 || %6==0.3)
|
||
quote
|
||
| relative bremer support |;
|
||
else
|
||
if (%6==0.2 || %6==0.3)
|
||
quote
|
||
| bremer support |;
|
||
end end
|
||
else
|
||
if ((%6>=1) && (%6<2))
|
||
if (%6==1.1 || %6==1.3)
|
||
quote
|
||
| relative bremer support |;
|
||
else
|
||
if (%6==1.2 || %6==1.3)
|
||
quote
|
||
| bremer support |;
|
||
end end
|
||
quote
|
||
| jackknifing |;
|
||
else
|
||
if ((%6>=2) && (%6<3))
|
||
if (%6==2.1 || %6==2.3)
|
||
quote
|
||
| relative bremer support |;
|
||
else
|
||
if (%6==2.2 || %6==2.3)
|
||
quote
|
||
| bremer support |;
|
||
end end
|
||
quote
|
||
| bootstrap |;
|
||
else
|
||
if ((%6>=3) && (%6<4))
|
||
if (%6==3.1 || %6==3.3)
|
||
quote
|
||
| relative bremer support |;
|
||
else
|
||
if (%6==3.2 || %6==3.3)
|
||
quote
|
||
| bremer support |;
|
||
end end
|
||
quote
|
||
| jackknifing, bootstrap |;
|
||
else
|
||
if ((%6>=4) && (%6<5))
|
||
if (%6==4.1 || %6==4.3)
|
||
quote
|
||
| relative bremer support |;
|
||
else
|
||
if (%6==4.2 || %6==4.3)
|
||
quote
|
||
| bremer support |;
|
||
end end
|
||
quote
|
||
| symmetric resampling |;
|
||
else
|
||
if ((%6>=5) && (%6<6))
|
||
if (%6==5.1 || %6==5.3)
|
||
quote
|
||
| relative bremer support |;
|
||
else
|
||
if (%6==5.2 || %6==5.3)
|
||
quote
|
||
| bremer support |;
|
||
end end
|
||
quote
|
||
| jackknifing, symmetric resampling |;
|
||
else
|
||
if ((%6>=6) && (%6<7))
|
||
if (%6==6.1 || %6==6.3)
|
||
quote
|
||
| relative bremer support |;
|
||
else
|
||
if (%6==6.2 || %6==6.3)
|
||
quote
|
||
| bremer support |;
|
||
end end
|
||
quote
|
||
| bootstrap, symmetric resampling |;
|
||
else
|
||
if ((%6>=7) && (%6<8))
|
||
if (%6==7.1 || %6==7.3)
|
||
quote
|
||
| relative bremer support |;
|
||
else
|
||
if (%6==7.2 || %6==7.3)
|
||
quote
|
||
| bremer support |;
|
||
end end
|
||
quote
|
||
| jackknifing, bootstrap, symmetric |
|
||
| resampling |;
|
||
else
|
||
errmsg Mistake %6 value;
|
||
end end end end end end end end
|
||
else
|
||
quote
|
||
| relative bremer support, bremer |
|
||
| support, jackknifing, bootstrap, |
|
||
| symmetric resampling |;
|
||
end
|
||
|
||
quote
|
||
| The file `apo.svg` contains the |
|
||
| tree with apomorphy mapping |
|
||
| The file `report.log` contains the |
|
||
| CI RI TL publish-needed info |
|
||
| The file `resample/apo.log` contain the |
|
||
| tree tags in text |
|
||
| The file `homo.log` contain the report |
|
||
| of character homoplasy |
|
||
| The file `eiw.log` contain the report |
|
||
| of character concavities |
|
||
| The file `winclada.tre` can be |
|
||
| converted by tnt2winclada |
|
||
\----------------------------------------------/;
|
||
|
||
/*Quit*/
|
||
zzz;
|