Compare commits
3 commits
5a7faee66e
...
05ba3e3fc4
Author | SHA1 | Date | |
---|---|---|---|
05ba3e3fc4 | |||
12829a96af | |||
ce2cc5a662 |
2 changed files with 283 additions and 40 deletions
40
README.md
40
README.md
|
@ -12,11 +12,11 @@ The TNT script follows MIT and part of script is from setk.run belonging to Salv
|
|||
|
||||
## Functions
|
||||
|
||||
- Estimate implied weighting K value.
|
||||
- Extended implied weighting with K value setting (default, default K=12) or implighted weighting with K value setting (default K=12) or equal weighting.
|
||||
|
||||
- Search trees via TBR Mult (1000 times) and Xmult (use random sectorial searches, produce 1,000 hits to best length and stop, 10 cycles of drifting, ratchet and fusing).
|
||||
- Search trees via implicit enumeration (ntax<=25, but not for eiw) or TBR Mult (1000 times) (25<ntax<75, also for ntax<=25 with eiw) or Mult and Xmult (use random sectorial searches, produce 1,000 hits to best length and stop, 10 cycles of drifting, ratchet and fusing) (ntax>=75).
|
||||
|
||||
- Perform Strict consensus.
|
||||
- Perform Strict consensus / Majority-rule consensus / Half strict consensus.
|
||||
|
||||
- Calculate Relative Bremer support, jackknifing (1,000 times), and bootstrap (1,000 times).
|
||||
|
||||
|
@ -26,16 +26,42 @@ The TNT script follows MIT and part of script is from setk.run belonging to Salv
|
|||
|
||||
## Options
|
||||
|
||||
For Windows users
|
||||
|
||||
```
|
||||
tnt run guoyi.run filename datatype weight 0/K cons resample;
|
||||
```
|
||||
|
||||
For Linux and Mac users
|
||||
|
||||
```
|
||||
tnt run guoyi.run filename datatype weight 0/K cons resample,
|
||||
```
|
||||
|
||||
- datatpye should be `32`, `dna`, `prot`, `num`
|
||||
|
||||
- num=number, dna=DNA, prot=protein, 32=max number allowed (default)
|
||||
|
||||
- weight should be `iw`, ew, `eiw`
|
||||
|
||||
- iw=implied weight, ew=equal weight, eiw=extended implied weight (default) N.B. ew must followed 0 (the K value position for ew iw and eiw)
|
||||
|
||||
- cons should be str, mjr, hlf
|
||||
|
||||
- mjr=majority rule, hlf=half, str=strict (default)
|
||||
|
||||
- resample should be sum of what you want
|
||||
|
||||
- jak=1, boot=2, relative bremer=4 i.e. jak+boot+bremer=7 (default)
|
||||
|
||||
## Results
|
||||
|
||||
- Results instructions are at the end of `tnt.log`.
|
||||
|
||||
- `trees.tre`, `resample.tre` are trees with taxname.
|
||||
|
||||
- `trees_no.tre`, `resample_no.tre` are trees without taxname.
|
||||
|
||||
- `nelsen` can be replaced by `majority`.
|
||||
|
||||
- `xmult` and `mult` replications and hold trees number can be adjusted.
|
||||
|
||||
- `winclada.tre` can be transferred to the acceptable format for WinClada by tnt2winclada and the output file can be put into Winclada with your tnt matrix file `filename` for mapping apomorphic characters and homoplasy.
|
||||
|
||||
## Cite
|
||||
|
|
277
guoyi.run
277
guoyi.run
|
@ -1,47 +1,184 @@
|
|||
log tnt.log;
|
||||
macro=; taxname+1000; taxname=; mxram 10240; nstates 32; nstates NOGAPS;
|
||||
|
||||
macro=;
|
||||
|
||||
/*Arguments*/
|
||||
if ( argnumber != 1 )
|
||||
if ( (argnumber == 0) | (argnumber >=7) )
|
||||
silent -console;
|
||||
quote - /--------------------------------------------------\;
|
||||
quote - /----------------------------------------------------\;
|
||||
quote - | GUOYI TNT SCRIPT 2022-2023 MIT |;
|
||||
quote - | You need to give your filename |;
|
||||
quote - | shell> tnt run guoyi.tnt filename, (Linux & Mac) |;
|
||||
quote - | shell> tnt run guoyi.run filename; (Windows) |;
|
||||
quote - \--------------------------------------------------/;
|
||||
quote - | shell> tnt run guoyi.run filename(semicolon) (Win) |;
|
||||
quote - | ============================== |;
|
||||
quote - | === Parameters Details === |;
|
||||
quote - | ============================== |;
|
||||
quote - | filename datatype weight 0/K cons resample |;
|
||||
quote - | - datatpye should be 32, dna, prot, num |;
|
||||
quote - | num=number, dna=DNA, prot=protein |;
|
||||
quote - | 32=max number allowed (default) |;
|
||||
quote - | - weight should be iw, ew, eiw |;
|
||||
quote - | iw=implied weight, ew=equal weight |;
|
||||
quote - | eiw=extended implied weight (default) |;
|
||||
quote - | N.B. ew must followed 0 |;
|
||||
quote - | - cons should be str, mjr, hlf |;
|
||||
quote - | mjr=majority rule, hlf=half |;
|
||||
quote - | str=strict (default) |;
|
||||
quote - | - resample should be sum of what you want |;
|
||||
quote - | jak=1, boot=2, relative bremer=4 |;
|
||||
quote - | i.e. jak+boot+bremer=7 (default) |;
|
||||
quote - \----------------------------------------------------/;
|
||||
proc/;
|
||||
end
|
||||
|
||||
/*Basic settings*/
|
||||
taxname+1000;
|
||||
taxname=;
|
||||
mxram 10240;
|
||||
if(argnumber>=2)
|
||||
nstates %2;
|
||||
else
|
||||
nstates 32;
|
||||
end
|
||||
nstates NOGAPS;
|
||||
|
||||
/*Set K*/
|
||||
if(argnumber>2 )
|
||||
if (eqstring [ %3 ew ])
|
||||
quote Equal weighting is used;
|
||||
if (argnumber>=4)
|
||||
if (%4!=0)
|
||||
quote Warning: equal weighting %4 should be fullfilled with 0;
|
||||
end
|
||||
end
|
||||
end
|
||||
if ((eqstring [ %3 iw ]) | (eqstring [ %3 eiw ]))
|
||||
if (argnumber>=4)
|
||||
piwe=%4;
|
||||
else
|
||||
piwe=12;
|
||||
end
|
||||
end
|
||||
else
|
||||
piwe=12;
|
||||
end
|
||||
|
||||
/*Reopen tnt*/
|
||||
procedure %1;
|
||||
hold 10000;
|
||||
|
||||
/*Report what will be done*/
|
||||
quote - /-----------------------------------------------\;
|
||||
quote - | Implied weighting will be estimated. |;
|
||||
if(argnumber>2)
|
||||
if (eqstring [ %3 iw ])
|
||||
if (argnumber>3)
|
||||
quote - | Implied weighting will be used, K is %4. |;
|
||||
else
|
||||
quote - | Implied weighting will be used, K is 12. |;
|
||||
end
|
||||
end
|
||||
if (eqstring [ %3 eiw ])
|
||||
if (argnumber>3)
|
||||
quote - | Ex-implied weighting will be used, K is %4. |;
|
||||
else
|
||||
quote - | Ex-implied weighting will be used, K is 12. |;
|
||||
end
|
||||
end
|
||||
if (eqstring [ %3 ew ])
|
||||
quote - | Equal weighting will be used. |;
|
||||
end
|
||||
else
|
||||
quote - | Ex-implied weighting will be used, K is 12. |;
|
||||
end
|
||||
if (ntax<=25)
|
||||
quote - | Implicit enumeration will be performed. |;
|
||||
end
|
||||
if ((ntax>25) && (ntax < 75))
|
||||
quote - | TBR Mult will be performed. |;
|
||||
end
|
||||
if (ntax>=75)
|
||||
quote - | TBR Mult and Xmult will be performed. |;
|
||||
end
|
||||
if (argnumber>=5)
|
||||
if (eqstring [ %5 mjr ])
|
||||
quote - | Majority-rule consensus will be used. |;
|
||||
end
|
||||
if (eqstring [ %5 hlf ])
|
||||
quote - | Half strict consensus will be used. |;
|
||||
end
|
||||
if(eqstring [ %5 str ])
|
||||
quote - | Strict consensus will be used. |;
|
||||
end
|
||||
end
|
||||
if (argnumber<5)
|
||||
quote - | Strict consensus will be used. |;
|
||||
end
|
||||
if (argnumber>=6)
|
||||
if (%6==1)
|
||||
quote - | Jackknifing |;
|
||||
end
|
||||
if (%6==2)
|
||||
quote - | Bootstrap |;
|
||||
end
|
||||
if (%6==3)
|
||||
quote - | Jackknifing and bootstrap |;
|
||||
end
|
||||
if (%6==4)
|
||||
quote - | Relative bremer support |;
|
||||
end
|
||||
if (%6==5)
|
||||
quote - | Jackknifing and relative bremer support |;
|
||||
end
|
||||
if (%6==6)
|
||||
quote - | Bootstrap and relative bremer support |;
|
||||
end
|
||||
if (%6==7)
|
||||
quote - | Jackknifing, bootstrap and relative bremer |;
|
||||
end
|
||||
end
|
||||
if (argnumber<6)
|
||||
quote - | Jackknifing, bootstrap and relative bremer |;
|
||||
end
|
||||
quote - | will be shown on the resample.svg. |;
|
||||
quote - | Apomorphic characters mapping will be shown |;
|
||||
quote - | on the apo.svg and saved to apo*.tre. |;
|
||||
quote - | TL, CI and RI will be calculated finally. |;
|
||||
quote - \------------------------------------------------/;
|
||||
|
||||
|
||||
/*Set K*/
|
||||
piwe=12;
|
||||
|
||||
/*Reopen tnt*/
|
||||
procedure %1;
|
||||
hold 10000;
|
||||
|
||||
/*Implied weighting settings*/
|
||||
xpiwe(*;
|
||||
piwe&;
|
||||
if(argnumber>2)
|
||||
if (eqstring [ %3 iw ])
|
||||
piwe&;
|
||||
end
|
||||
if (eqstring [ %3 eiw ])
|
||||
xpiwe(*;
|
||||
piwe&;
|
||||
end
|
||||
else
|
||||
xpiwe(*;
|
||||
piwe&;
|
||||
end
|
||||
|
||||
/*Search trees*/
|
||||
mult=replic 1000 tbr hold 1000;
|
||||
bbreak=tbr fill;
|
||||
sect: slack 1000;
|
||||
xmult=hit 1000 noupdate replications 20 drift 10 ratchet 10 fuse 10 hold 1 keepall;
|
||||
if(eqstring [ %3 ew ] | eqstring [ %3 iw ] )
|
||||
if (ntax<=25)
|
||||
ienum;
|
||||
else
|
||||
mult=replic 1000 tbr hold 1000;
|
||||
bbreak=tbr fill;
|
||||
if (ntax>74)
|
||||
sect: slack 1000;
|
||||
xmult=hit 1000 noupdate replications 20 drift 10 ratchet 10 fuse 10 hold 1 keepall;
|
||||
end
|
||||
end
|
||||
else
|
||||
mult=replic 1000 tbr hold 1000;
|
||||
bbreak=tbr fill;
|
||||
if (ntax>74)
|
||||
sect: slack 1000;
|
||||
xmult=hit 1000 noupdate replications 20 drift 10 ratchet 10 fuse 10 hold 1 keepall;
|
||||
end
|
||||
end
|
||||
|
||||
/*Export trees*/
|
||||
export= trees.tre;
|
||||
|
@ -52,7 +189,19 @@ tsave = trees.ctf;
|
|||
tsave *= trees.tnt.tre;
|
||||
|
||||
/*Consensus tree*/
|
||||
nelsen *;
|
||||
if (argnumber<=4)
|
||||
nelsen *;
|
||||
else
|
||||
if (eqstring [ %5 str ])
|
||||
nelsen *;
|
||||
end
|
||||
if (eqstring [ %5 mjr ])
|
||||
majority *;
|
||||
end
|
||||
if (eqstring [ %5 hlf ])
|
||||
comcomp *;
|
||||
end
|
||||
end
|
||||
|
||||
/*Tree vault store*/
|
||||
hold /+0;
|
||||
|
@ -103,15 +252,40 @@ ttags-;
|
|||
tchoose/;
|
||||
ttags=;
|
||||
ttags]; /*in one line*/
|
||||
resample jak replications 1000 from 0;
|
||||
|
||||
resample boot replications 1000 from 0;
|
||||
/* jak=1, boot=2, bremer=4, */
|
||||
/* jak+boot=3, */
|
||||
/* jak+brember=5, boot+bremer=6, */
|
||||
/* jak+boot+bremer=7, */
|
||||
|
||||
sub 1; hold +1000; bbreak=fill;
|
||||
sub 3; hold +3000; bbreak=fill;
|
||||
sub 5; hold +5000; bbreak=fill;
|
||||
sub 7; hold +7000; bbreak=fill;
|
||||
bsupport[;
|
||||
if (argnumber<=5)
|
||||
/*jakknifing*/
|
||||
resample jak replications 1000 from 0;
|
||||
/*bootstrap*/
|
||||
resample boot replications 1000 from 0;
|
||||
/*relative bremer*/
|
||||
sub 1; hold +1000; bbreak=fill;
|
||||
sub 3; hold +3000; bbreak=fill;
|
||||
sub 5; hold +5000; bbreak=fill;
|
||||
sub 7; hold +7000; bbreak=fill;
|
||||
bsupport[;
|
||||
else
|
||||
if((%6==1) | (%6==3) | (%6==5) | (%6==7))
|
||||
resample jak replications 1000 from 0;
|
||||
end
|
||||
|
||||
if((%6==2) | (%6==3) | (%6==6) | (%6==7))
|
||||
resample boot replications 1000 from 0;
|
||||
end
|
||||
|
||||
if((%6==4) | (%6==5) | (%6==6) | (%6==7))
|
||||
sub 1; hold +1000; bbreak=fill;
|
||||
sub 3; hold +3000; bbreak=fill;
|
||||
sub 5; hold +5000; bbreak=fill;
|
||||
sub 7; hold +7000; bbreak=fill;
|
||||
bsupport[;
|
||||
end
|
||||
end
|
||||
|
||||
/*Export consensus tree with supports*/
|
||||
ttags & resample.svg thickness 7 italics fontsize 15;
|
||||
|
@ -151,9 +325,52 @@ quote - | only readable for TNT |;
|
|||
quote - | The file `*.tnt.tre` contain |;
|
||||
quote - | is the ctf file with taxname |;
|
||||
quote - | The file `resample.svg` contain |;
|
||||
|
||||
|
||||
if (argnumber>=5)
|
||||
if(eqstring [ %5 mjr ])
|
||||
quote - | majority rule consensus tree with |;
|
||||
end
|
||||
if(eqstring [ %5 hlf ])
|
||||
quote - | half strict consensus tree with |;
|
||||
end
|
||||
if(eqstring [ %5 str ])
|
||||
quote - | strict consensus tree with |;
|
||||
quote - | relative jak, boot and bremer |;
|
||||
quote - | support on the tree |;
|
||||
end
|
||||
else
|
||||
quote - | strict consensus tree with |;
|
||||
end
|
||||
|
||||
if (argnumber>=6)
|
||||
if (%6==1)
|
||||
quote - | jackknifing |;
|
||||
end
|
||||
if (%6==2)
|
||||
quote - | bootstrap |;
|
||||
end
|
||||
if (%6==3)
|
||||
quote - | jackknifing and bootstrap |;
|
||||
end
|
||||
if (%6==4)
|
||||
quote - | relative bremer support |;
|
||||
end
|
||||
if (%6==5)
|
||||
quote - | jackknifing and relative bremer |;
|
||||
quote - | support |;
|
||||
end
|
||||
if (%6==6)
|
||||
quote - | bootstrap and relative bremer |;
|
||||
quote - | support |;
|
||||
end
|
||||
if (%6==7)
|
||||
quote - | jackknifing, bootstrap and |;
|
||||
quote - | relative bremer support |;
|
||||
end
|
||||
else
|
||||
quote - | jackknifing, bootstrap and |;
|
||||
quote - | relative bremer support |;
|
||||
end
|
||||
|
||||
quote - | The file `apo.svg` contain the |;
|
||||
quote - | tree with apomorphy mapping |;
|
||||
quote - | The file `winclada.tre` can be |;
|
||||
|
|
Loading…
Reference in a new issue