.TH ted 1L "July 1991" "MRC LMB" "LOCAL" .SH NAME ted \- trace editor .SH SYNOPSIS .B ted [( .B -ABI\||\|-ALF\||\|-plain ) .I tracefilename [ .B -baseNum .I number ] .B [ .B -mag .I number ( 1 to 100 ) ] .B [ .B -bottom .I number (1(true) or 0(false)) .B ] .B [ .B -astring .I nucleotide-string ]] .B [ .B -enzyme .I 5' cutting sequence ] .B [ .B -raw .I filename (to be placed at head of xdap compatible .seq file) .B ] [ .B -output .I outputfilename ] .SH DESCRIPTION .B ted is a simple prototype editor for traces produced from automatic sequencing machines. It allows the traces (from the ABI or ALF sequencing machines) produced to be displayed along with the machines interpretation of these into bases and an initially identical sequence which can be edited by the user. A cutoff region can be defined at both ends. The edited and clipped list of bases can then be written out. .LP When initially run, .B ted displays the trace file .I tracefilename (if given) of the specified format centered on the base number .I baseNum (if given). If no file is provided, .B ted initially displays nothing. .LP The display consists of the control panel and the synchronized view of the base position information, original and edited sequence data, and graphical representation of the trace (with each nucleotide's trace being represented by a different color). The control panel allows the user to read in new trace files (in either bottom or top strand orientation) as well as to search for a string of nucleotides or a certain base position. The information button brings up signal strength and average spacing for ABI files. Scroll bars allow the user to adjust the magnification of or scroll through the sequence and trace data. The user may also choose to change the vertical magnification of the trace data. Further, sequence on the head (vector) or tail (uncertain data) of the sequence may be ``cutoff'' using the adjust left and right cutoff buttons. Bases can be inserted, deleted, or replaced as with any ordinary word-processor in the sequence data window. Finally, the sequence may be written to an ascii file using the output button on the control panel. The output filename is specified in a dialogue, but a default value of inputfilename.seq is provided or the default value can be given with the .I outputfilename argument. .LP A simple help system is provided. .SH FILES .PD 0 .TP 20 .B ted.help Text provided in the help window. .TP .B /usr/lib/X11/app-defaults/Xted Default application resources. .SH ENVIRONMENT .TP 20 .SB XFILESEARCHPATH Specifies the locations where .B ted.help is sought. If this is not defined, .B ted.help must be in the .B /usr/lib/X11/app-defaults directory. .SH AUTHORS Tim Gleeson, LaDeana Hillier, Simon Dear.