From f8b7bfff1b9e933830c089d9d7f24a7349326363 Mon Sep 17 00:00:00 2001 From: starsareintherose Date: Sat, 4 Dec 2021 05:07:58 +0000 Subject: [PATCH] init --- README.txt | 296 + ReleaseNotes | 190 + SequenceLibraries | 420 + Staden_install-alpha | 453 + Staden_install-dec | 453 + Staden_install-sgi | 453 + Staden_install-solaris | 453 + Staden_install-sun | 453 + Version-1993.0.7 | 91 + bin/alfsplit | Bin 0 -> 106496 bytes bin/bap | Bin 0 -> 1236992 bytes bin/convert | Bin 0 -> 172032 bytes bin/cop | Bin 0 -> 196608 bytes bin/cop-bap | Bin 0 -> 196608 bytes bin/dap | Bin 0 -> 1187840 bytes bin/frog | Bin 0 -> 106496 bytes bin/getABISampleName | Bin 0 -> 98304 bytes bin/gip | Bin 0 -> 262144 bytes bin/lip | Bin 0 -> 360448 bytes bin/makeSCF | Bin 0 -> 172032 bytes bin/mep | Bin 0 -> 401408 bytes bin/nip | Bin 0 -> 1261568 bytes bin/nipf | Bin 0 -> 458752 bytes bin/nipl | Bin 0 -> 548864 bytes bin/pip | Bin 0 -> 745472 bytes bin/pipl | Bin 0 -> 516096 bytes bin/rep | Bin 0 -> 303104 bytes 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userdata/HINW.004 create mode 100644 userdata/HINW.009 create mode 100644 userdata/HINW.010 create mode 100644 userdata/HINW.016 create mode 100644 userdata/HINW.017 create mode 100644 userdata/HINW.025 create mode 100644 userdata/HINW.028 create mode 100644 userdata/HINW.031 create mode 100644 userdata/README create mode 100644 userdata/atpase.dat create mode 100644 userdata/blue.vec create mode 100644 userdata/lambda.seq create mode 100644 userdata/lorist6.vep create mode 100644 userdata/m13mp18.vec create mode 100644 userdata/m13mp7.seq create mode 100644 userdata/newtrna.pat create mode 100644 userdata/pjb8.vep create mode 100644 userdata/prokprom.seq create mode 100644 userdata/puc18.vec diff --git a/README.txt b/README.txt new file mode 100644 index 0000000..f8c2986 --- /dev/null +++ b/README.txt @@ -0,0 +1,296 @@ + General Information + (Not for the faint hearted) + + 30 September 1992 + + +0. Introduction +--------------- + +This document contains information on the following subjects: + + 1. Installing the Staden Package on SPARCstations and DECstations + 2. Installing the Staden Package on Other Machines + 3. A Quick Guide to What's on the Release Tape + 4. Overview of Data Flow During Sequence Assembly + 5. Acknowledgements + + + +1. Installing the Staden Package on SPARCstations and DECstations +----------------------------------------------------------------- + +We are endeavouring to make the installation of the Staden Package as +quick and as easy as possible. In this current release we provide +statically linked sparc and mips executables as well as all sources. + +To install the package: + +1) Create a new directory for the software. You may have to log on as +superuser to do this. + + % mkdir -p /home/BioSW/staden + +2) Place the distribution tape in the drive and down load the package: + + -sun- + % tar xvf /dev/rst0 + ...system messages... + + -dec- + % tar xvf /dev/rmt0h + ...system messages... + +3) Users of the C Shell should add the following to his/her .login +file: + + setenv STADENROOT /home/BioSW/staden + source $STADENROOT/staden.login + +Users of the Bourne shell should add the following to their .profile +file: + + STADENROOT=/home/BioSW/staden + export STADENROOT + . $STADENROOT/staden.profile + + +4) When the user next logs onto the work station the required +initialisation will automatically be performed, and the programs in +the Staden package can be run. Refer to the help/*.MEM files for +information on the various program. (eg help on xdap is in +help/DAP.MEM) + + +2. Installing the Staden Package on Other Machines +-------------------------------------------------- + +This is a little more difficult as you will need to remake all the +executables. Your system configuration may also mean that some changes +will need to be made, though hopefully only to makefiles. We provide +a script to aid installation (we hope!), but you may prefer to make +all the components manually. + +To remake the Staden package you will require the following: + 1) A Fortran77 compiler + 2) An ANSI C compiler + 3) X11 Release 4, including the Athena Widget libraries. + +Start by following step 1 through 3 above, to unload the sources and +perform initialisations. Read the rest of this document and the other +help files. Look at the make files. Follow your nose! + +If you have any problems or successes porting our software to other +platforms we would love to hear from you. We would also appreciate +receiving your general comments on the package. + +Rodger Staden (principle author) + phone: +44 223 402389 email: rs@mrc-lmba.cam.ac.uk + post: MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, U.K. +Simon Dear: + phone: +44 223 402266 email: sd@mrc-lmba.cam.ac.uk + post: MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, U.K. +James Bonfield: + phome: +44 223 402499 email: jkb@mrc-lmba.cam.ac.uk + post: MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, U.K. + + + +3. A Quick Guide to What's on the Release Tape +---------------------------------------------- + +The directory structure on this tape is very important. Once set up, the Staden +package expects things to be in a predefined place. The root directory +of the structure is referred to by the environment variable +STADENROOT. Below this there should be at least the following: + +1) bin/ +All executable files and scripts should be in this directory. +$STADENROOT/bin is added to the search path by the script staden.login +(or staden.profile if you are using the Bourne Shell). Though you are +not forced to keep programs here, we find it is the simplest place to +keep them. + +2) help/ +All on-line help files are in this directory. Files of the form *.MEM +or *.mem are formatted ascii files and can be printed for personal +reference. The script staden.login sets up many environment variables +that refer to files in this directory, as well as modifying +XFILESEARCHPATH, which is used by X programs. + +3) manl/ +Local manual pages for ted and the staden package are in this directory. The +environment variable MANPATH is modified in staden.login to search +here too. + +4) staden.login and staden.profile +These two files are scripts to set up environment variables required +by the Staden package. C Shell users should source staden.login from +their .login file, and Bourne Shell users should "source" staden.profile +from their .profile directory. See "Installing the Staden Package on +SPARCstations and DECstations", Part 3. + +5) tables/ +Configuration files for the Staden package are in this directory. +Various environment variables are set in staden.login to refer to +files in this directory. + +Also of use are the following: + +doc/ - Miscellaneous documentation. +userdata/ - Sample databases +src/ - program sources +ReleaseNotes - Notes on this and future releases +Staden_install - Installation script +SequenceLibraries - Notes on the use and installation of sequence libraries + + +Program Sources +--------------- + +All the program sources are found in the directories in $STADENROOT/src: + +0) Misc/ +Sources for a library of useful routines used by the staden package. +** Should be made before the programs in staden/ ** + +1) staden/ +Sources for the Staden suite: mep, xmep, nip, xnip, nipl, pip, xpip, +pipl, sap (now superseded by dap), xsap (now superceded by xdap), sip, +xsip, sipl, dap, xdap, splitp1, splitp2, splitp3, gip and convert_project. + +2) ted/ +Sources for the trace display and sequence editing program ted. + +3) abi/ +Sample scripts and programs for handling ABI 373A data files. + +4) alf/ +Sample scripts and programs for handling Pharmacia A.L.F. data files. + +Each directory has appropriate makefiles and README files. + + + +4. Overview of Data Flow During Sequence Assembly +------------------------------------------------- + +During a sequence assembly project the data can enter the sequence +assembly program from various routes (See Figure below). + + + + Fluorescent Based + Sequencing Machine + Chromatogram Autoradiogram + + ABI 373A Pharmacia A.L.F. | + | | | + | | | + | alfsplit | + | | | + +--------+--------+ | + | | + | | + ted (gip) + | | + +----------------+----------------+ + | + | + xdap + + + Figure 1: Data Flow Through The Staden Suite + + +The Pharmacia A.L.F. data files in their original format consist of +one file for the (up to 10) samples that were on the gel. The program +alfsplit divides the file up so that each sample is in a file of +its own. From then on each gel reading can be handled individually. +Whether these files can be transferred back to the Compaq for +reprocessing is unknown. + +All data from fluorescent based sequencing machines must pass through +the trace editing program ted. Ted allows data vector sequence at the +5' end and unreliable data at the 3' end to be clipped. The sequence +can be edited if desired, though we should stress that this is NOT +RECOMMENDED when used in conjunction with xdap. Ted translates all +Pharmacia A.L.F. uncertainty codes to a hyphen ("-") and outputs the +clipped sequence, along with additional information on the position +and content of cutoffs, to a file. + +People wanting to use xdap with ABI and Pharmacia files, but who have +written their own trace clipping software should be aware that xdap +requires information to be passed in the sequence file so that +traces can be displayed. You may want to modify your software to be +compatible with our file format. The file consists of four parts: + + 1) Cut off information (Optional). + Format is ";%6d%6d%6d%-4s%-16s", where + field 1 = total number of bases called + 2 = number of bases in the clipped sequence at the 5' end + 3 = number of bases in the sequence in this file + 4 = type of trace file. + "ALF " - Pharmacia A.L.F. + "ABI " - ABI 373A + "SCF " - SCF + "PLN " - Text only + 5 = name of trace file. + + 2) Content of the clipped sequence at the 5' end (Optional). + The sequence can extend over several lines. Each line must + begin with ";<" and should be less than 80 characters in + length. + + 3) Content of the clipped sequence at the 3' end (Optional). + The sequence can extend over several lines. Each line must + begin with ";>" and should be less than 80 characters in + length. + + 4) Initial tags for the sequence (Optional) + Format is: ";;%4s %6d %6d %s\n", where + field 1 = type of tag to be created (see $STADTABL/TAGDB) + 2 = position of tag + 3 = length of tag + 4 = annotation for tag (optional) + This feature is only available in the program xbap, which + at the time of writing is not yet being distributed with + the package. + + 5) The sequence, which can extend over several lines. Each + line should be less than 80 characters in length. + +Here is a sample file: + +; 660 55 450ABI a21d12.s1RES +;-GATAAGCTGATTTG-TTT-CCATTATGGC-GGTTTGAGCCTC-G-GGTC +;>GACCACTCGGTGTGCCAGGAAGGGGTCTGAAATTGAATGGGTTATCACTA +;>GGCGACGTTT--TTTTCAAATTCCGGGCTAAATTTTACGGC-GGA-CGGT +;>TCCG- +;;COMM 1 10 M13mp18 subclone +CAAGACATTTTGAAATACTTGGAATACTGAATCCAAGATGTGGAACATTA +GACATATCCGTGTGCTCAACAATCGACATTTGATCCACTGATGAAAATGT +TCTTCGTTTAGAATTTCTCATAGCATCAGCCACTTTTGCATAATACTCGA +TTGAAGGTTCATGGAAAAAGCTGCGTAGAAGGCATGTCATTGTGCTTACG +AGCCATTTCGGATATCTTGTGAATTTAGCAGGAAGTTCTGTAACTGGTTG +GAATTCAAATATATCAGTTCTTCTTCCTGGATCTCGTCCTTTTTGCACTA +AAACCATTGCGATTGCATCCGGATTCTGAGTAAGAGCCACTACAGCTTTA +TGATACAGGCTCTTGTTATTCCTTTCGTGCTCGAATGGGAACTTTCCAGT +GGCACAAAAATATAGTGTACATCCCAGAGCCCATAGATCACATGTTCCGA + + + +5. Acknowledgements + +We would like to thank Applied Biosystems, Inc. and Pharmacia LKB +Biotechnology for their cooperation in agreeing to our routines +accessing the data files of their fluorescent sequencing machines. + +373A sequence data file formats are the exclusive property of Applied +Biosystems, Inc. + +ALF sequence data file formats are the exclusive property of Pharmacia +LKB Biotechnology, Inc. + diff --git a/ReleaseNotes b/ReleaseNotes new file mode 100644 index 0000000..896cae1 --- /dev/null +++ b/ReleaseNotes @@ -0,0 +1,190 @@ + Release Notes for Staden Package 1992.3 + --------------------------------------- + + + Installation guide + ------------------ + +The file doc/install.PS contain installation instructions. + + + Manual for the Staden Package + ----------------------------- + +There is now a 135 page manual on the Staden Package. It is currently +being distributed on a Word4 document on a Macintosh floppy disk. + + + Feedback and bug reports + ------------------------ + +We welcome comments and suggestions on all aspects of the package and are +best contacted by email: rs@uk.ac.cam.mrc-lmb and sd@uk.ac.cam.mrc-lmb. +All abnormal terminations are bugs and we would like to be told of them +so they can be fixed. We recommend that you request an update at least once +a year as the package is evolving very rapidly. + +Note due to popular demand we have decided to release new routines earlier +than in the past so please report bugs. The documentation for additions may +be sparser than before, or non-existent, but if there is something with which +you need help, email us. + + + Changes this release + -------------------- + + + The assembly programs bap and xbap heve several new functions: + 1. Find single stranded regions and try to fill them with "hidden" + data from the adjacent readings. + 2. Find single stranded regions (includes ends of contigs) and + select primers and templates for double stranding them (joining + them). + 3. Pre assembly screening for readings to find those that align + best. Optionally the hidden data can also be included in the + comparison (part of assembly function). + 4. Find pairs of readings taken from opposite ends of the same + template (ie forward and reverse read pairs). List or plot their + positions. + 5. A new function to check that readings have been assembled into + the correct positions. It aligns the hidden (previously termed "unused") + parts of readings with the consensus they overlap to see how well + they align. Poor alignments are reported. + 6. During assembly each reading is now allowed to match up to 100 + different places. + + It might be guessed from the above that we are trying to improve our + ability to deal with the assembly of human data. Hence, also the next + addition. + + A new experimental program (rep) for screening readings for Alu + sequences prior to assembly. The Alu containing segments are tagged + so they can be seen in the contig editor. A library of Alu sequences + is included in /tables/alus. The program is quite slow as it compares + each reading in both orientations with all of the Alu sequences (126 + of them) in order to find the best match. Only time and more data will + tell how sensitive it is, and whether the current default score 0f 0.6 + is "correct". BEWARE rep modifies the original reading files to include + the tag information. The only information is in /help/alu.help + + A new program for extracting sets of sequences and their annotations + from the sequence libraries (lip). The only information is in + /help/lip.help + + Changes to the xterm userinterface. These routines have been completely + rewritten. One addition is that now ?? in response to a question will + allow the user to get help on any function in a program. help is also + improved in the x version. + + + Changes last release + -------------------- + + + DAP, XDAP have been replaced by BAP and XBAP (see below) + + A new function for examining repeats has been added to NIP + + A new repeat search has been added to SIP + + Some outputs have been changed to produce FASTA format files + instead of PIR. + + MEP now allows searches for motifs in which any 8 out of a string + of 20 can be switched on. + + The manual has been updated. + + Keyword and author searches on sequence libraries + + All programs that use the libraries can now perform author +and keyword searches on all libraries (only nip did so before). + + Postscript output + + All graphics can now be saved to disk in postscript form by +use of a sub-option in "Redirect output". + + + + Sequence assembly + +BAP, XBAP replace DAP and XDAP. A program to convert DAP databases to BAP +databases (convert) is included. BAP databases can contain up to 8000 readings +and a consensus of 500,000 bases. A minor edit and recompilation will allow +up to 99,999 readings. The space is used more efficiently now as the databases +grow as the number of readings increases. Reading names can be 16 characters +in length. In addition: + +1) Assembly is 4 times as fast as in the DAP. + +2) Find internal joins is 5 times as fast and now brings up the join editor +with the two contigs in the correct orientation and aligned. + +3) The assembly routines align pads better, plus a new automatic function can +also be used to align them prior to editing. + +4) The contig editor has been greatly speeded up and its functionality +has been enhanced. + +5) A routine for selecting oligos for primer walking is included. + +6) A new routine allows batches of readings to be removed from a database. + +7) We have also included routines for making SCF files, for getting the +sequence from SCF files, and one for marking the poor quality data in +readings. See the manual. + + Sequence library formats + + The standard sequence library indexing method is now that used on the +EMBL CD-ROM. The libraries (EMBL nucleotide and SWISSPROT protein) can be +left on the CD-ROM or copied to disk. We include in the package programs +for creating this type of index for EMBL updates, PIR in codata format, +NRL3D and GenBank. If the indexes are created all programs can read all +these libraries. Programs and scripts for this task are contained in the +directory indexseqlibs. + The keyword and author searches are particularly fast and the +keyword index is based on ALL text in the files - not just the keywords. + + Feature table formats + + The programs now use the new feature table format common to EMBL +and GenBank, but retain the old format for SWISSPROT which has not yet +changed. + + For details of the above see file SequenceLibraries. + + Pattern searches + + Pipl and Nipl now have the facility to find only the best scoring +match for each sequence. The prompt is "? report all matches", so typing +only return means all matches will be shown and typing n means only the +highest scoring will be reported. It is particularly useful when employed +to create alignments. The corresponding help file has not been updated. +Also to incorporate long unix file names the pattern files no longer include +the annotation "filename". + + + Nip + + Option 38 in nip "translate and list" has been removed as the the +more flexible routines of option 39 incorporate all its functionality. Many +options that relate to feature tables have been modified but their help files +are not yet up to date. + + + Vep + + A program (vep) for automatic excising of vector (either +sequencing vector or cosmid vector) sequences from readings is now +included in the package. + + + + + Rodger Staden, Simon Dear, James Bonfield + + + + diff --git a/SequenceLibraries b/SequenceLibraries new file mode 100644 index 0000000..5f22ff9 --- /dev/null +++ b/SequenceLibraries @@ -0,0 +1,420 @@ + Notes on library handling + ------------------------- + +Contents of this document: + +I) Introduction +II) Details of file organisation and use +III) Options currently available +IV) Installation guide +V) New feature table handling routines +VI) Indexing the sequence libraries + + + Section I Introduction + ---------------------- + +Available sequence libraries + +There are a number of different sequence libraries for nucleotide and protein: +PIR, GenBank, EMBL, Swissprot, and the Japanese Databank. Even after all the +years of their existence they still use different formats for their data. This +provides tedious and unrewarding work for software developers. Recently EMBL +and GenBank agreed a new and common way of writing their feature tables, which +is great help, although the rest of their format is different. Swissprot still +uses the old embl style feature table format and PIR yet another. + +All the libraries distribute their data on magnetic tapes and EMBL and GenBank +have started to distribute on cdrom. The EMBL cdrom also contains Swissprot. +The GenBank and EMBL cdroms use different formats and have different contents. +The EMBL cdrom has useful indexes sorted alphabetically: those for entry name +and accession number, brief descriptions, keywords and freetext indexes are +already available and others are expected. These indexes point to the data for +each entry, and can be used to extract the data for any entry quickly. + +Moving to unix + +The VAX version of our package used PIR format which meant reformatting all +libraries other than PIR into that format. This required, at least +temporarily, having space for two copies of the libraries, and quite a lot of +cpu time. The software for doing this was provided by PIR, and is very VAX +specific and hence will not run under unix. For the unix version of our package +I have decided to use the EMBL cdrom format and its indexes as the primary +format. The current programs also support the use of PIR format libraries +without indexes - ie just the sequence and annotation files. + +Indexing GenBank, EMBL updates, PIR and NRL3D + +We include programs to create indexes for the above libraries. See below and +the README file in indexseqlibs. The programs can read all the above libraries +once the indexes are created. The indexing programs index the data in its +distributed form: WE DO NOT REFORMAT OR COPY THE LIBRARIES but simply create +indexes to the original files. Obviously this saves a lot of disk space, and +for those content to use only embl and swissprot from the cdrom, almost no disk +space is required. We havent tried it yet, but for genbank on cdrom, the only +extra disk space required would be for the indexes. + + --------------------------------------------------------------------------- + + Section II Details of file organisation and use + ----------------------------------------------- + +The following strategy has been used to try to deal with alternate +and changing sequence library formats. + +1) libraries are described at several levels: + + a) the top level file is a list of available libraries which contains: + the library type, the name of the file containing the name of + each libraries individual files, and the prompt to appear on + the users screen: LTYPE LOGNAM PROMPT + + b) the file containing the names of the libraries individual files + contains flags to define the file types: FTYPE LOGNAM + + c) the individual library files + + + +2) libary types handled: + + a) EMBL/SWISSPROT in distributed format with cdrom index format + LTYPE = 'A' + b) GenBank in distributed format with cdrom index format LTYPE = 'C' + c) PIR/NRL3D in CODATA format with cdrom index format LTYPE = 'B' + d) PIR/NBRF .seq files can be read sequentially as "personal files + in PIR format" and do not appear in the list of available libraries. + e) FASTA format files can be read sequentially as "personal files + in FASTA format" and do not appear in the list of available + libraries. + +3) EMBL, SWISSPROT and other libraries for which EMBL-style indexes have been +created + + current file types: + + A division.lookup + B entryname.index + C accession.target + D accession.hits + E brief description + F freetext.target + G freetext.hits + H author.target + I author.hits + + + Library list +level 1 + | + | + ----------------------------------------------------------- + | | | + lib 1 file list lib 2 file list lib 3 file list +level 2 + | | + -------- --------- +level 3 + file 1 file 1 + file 2 file 2 + . . + file n file n + + --------------------------------------------------------------------------- + + +Example +------- + +Level 1 + + File name: sequence.libs + Environment variable: SEQUENCELIBRARIES + Contents: + +A EMBLFILES EMBL nucleotide library ! in cdrom format +C GENBFILES GenBank nucleotide library! +A SWISSFILES SWISSPROT protein library! in cdrom format +B PIRFILES PIR protein library! +B NRL3DFILES NRL3D protein library! + + Notes: + +The libraries have types A,B,C. The logical names are EMBLLIBDESCRP and +SWISSLIBDESCRP, etc and the prompts are 'EMBL nucleotide library' and +'SWISSPROT protein library', etc. Anything to the right of a ! is a comment. + +Level 2: the list of library files (using embl as an example) + + File name: embl.files + Environment variable: EMBLFILES + Contents: + +A EMBLDIVPATH/embl_div.lkp +B EMBLINDPATH/entrynam.idx +C EMBLINDPATH/acnum.trg +D EMBLINDPATH/acnum.hit +E EMBLINDPATH/brief.idx +F EMBLINDPATH/freetext.trg +G EMBLINDPATH/freetext.hit +H EMBLINDPATH/author.trg +I EMBLINDPATH/author.hit + + +Level 3: the sequence and annotation files (eg 15 for embl, 1 for swissprot). + + Paths and file names: + + EMBLPATH/bb.dat + EMBLPATH/fun.dat + EMBLPATH/inv.dat + EMBLPATH/mam.dat + EMBLPATH/org.dat + EMBLPATH/patent.dat + EMBLPATH/phg.dat + EMBLPATH/pln.dat + EMBLPATH/pri.dat + EMBLPATH/pro.dat + EMBLPATH/rod.dat + EMBLPATH/syn.dat + EMBLPATH/una.dat + EMBLPATH/vrl.dat + EMBLPATH/vrt.dat + +All files from the division lookup file down are exactly as they appear on the +cdrom. The division lookup file relates numbers stored in the indexes to +actual division (or data) files stored on the disk. We rewrite it so the +directory structure and file names can be chosen locally. Its format is +I6,1x,A. An example is given below. + + Division lookup file + + File name: STADTABL/embl_div.lkp + Environment variable path EMBLDIVPATH + Contents: + + 1 EMBLPATH/bb.dat + 2 EMBLPATH/fun.dat + 3 EMBLPATH/inv.dat + 4 EMBLPATH/mam.dat + 5 EMBLPATH/org.dat + 6 EMBLPATH/patent.dat + 7 EMBLPATH/phg.dat + 8 EMBLPATH/pln.dat + 9 EMBLPATH/pri.dat + 10 EMBLPATH/pro.dat + 11 EMBLPATH/rod.dat + 12 EMBLPATH/syn.dat + 13 EMBLPATH/una.dat + 14 EMBLPATH/vrl.dat + 15 EMBLPATH/vrt.dat + --------------------------------------------------------------------------- + + + Section III Options currently available + --------------------------------------- + +Facilities currently offered in nip,pip,sip,nipl,pipl,sipl: + + Get a sequence by knowing its entry name + Get a sequences' annotation by knowing its entry name + Get an entry name by knowing its accession number + Search the freetext index + Search the author index + +Facilities currently offered in nipl,pipl,sipl: + + Search whole library + Search only a list of entry names + Search all but a list of entry names + +Outline of each type of operation + +Looking for an entry by name: the programs will open the library description +file and read the names of its files and their file types. Then they will open +the entrynam.idx file, and find the sequence offset, annotation offset and +division number. Then open the division lookup file, find the file name for the +division required, open that file, seek to the required byte and get the data. + +Looking for an entry by accession number: the programs will open the library +description file and read the names of its files and their file types. Then +they open the acnum.trg and acnum.hit files. The acnum.trg file is read to find +the accession number and a pointer to the acnum.hit file and the number of +hits. That file is read and the corresponding entry names displayed. At +present no further action is performed, although I expect to list out the +titles for the entries found. + +Searching the whole of a library: the programs will open the library +description file and read the names of its files and their file types. Then +they open the division lookup file, read the names and numbers of the sequence +files, open all of them, then open the entryname file. Then the library is +processed sequentially by reading the entry names, their sequence offsets and +division numbers from the entry names file, and then the sequence from the +appropriate data file. + +Searching the whole of a library using a list of entry names to include: the +programs will open the library description file and read the names of its files +and their file types. Then they open the division lookup file, read the names +and numbers of the sequence files, open all of them, then open the entryname +file. Then the library is processed by reading the list of entry names and +finding the names in the entry names file to get their sequence offsets and +division numbers, and then the sequence from the appropriate data file. It will +stop when it reaches the end of the list of entry names. The list of entry +names can be in any order. + +Searching the whole of a library using a list of entry names to exclude: the +programs will open the library description file and read the names of its files +and their file types. Then they open the division lookup file, read the names +and numbers of the sequence files, open all of them, then open the entryname +file. Then the library is processed sequentially by reading the list of entry +names, reading the next entry in the entry names file to make sure it does not +match, then getting the sequence offsets and division numbers, and then the +sequence from the appropriate data file. If a the next name matches the name on +the list of entry names, it will be skipped, and the next name to exclude read. +If the list of excluded names is finished the rest of the library is searched +sequentially. The list of entry names must be in the same order as those in the +library (ie sorted alphabetically). + +Searching a whole library using a PIR format file is performed by reading it +sequentially. If as list of entry names is used it must be in the same order as +the entries in the library file. + --------------------------------------------------------------------------- + + + + + Section IV Installation guide + ----------------------------- + +EMBL CDROM + + The data can be left on the cdrom or copied to hard disk. The files +staden.login and staden.profile source the file $STADTABL/libraries.config.csh +and $STADTABL/libraries.config.sh respectively. Refer to this file to see what +is required to install, add or move a sequence library that you want to be used +by the programs. + +Other libraries (PIR, Genbank, EMBL updates) + +Create the indexes then edit the files that tell the programs where the data is +stored. The files staden.login and staden.profile source the file +$STADTABL/libraries.config Refer to this file to see what is required to +install, add or move a sequence library that you want to be used by the +programs. + + +------------------------------------------------------------------------------ + + + Section V New feature table handling facilities + ----------------------------------------------- + +As mentioned above EMBL and GenBank have recently introduced new feature tables +for annotating the sequences. They are a great improvement on the previous ones +and, among other things, now permit correct translation of spliced genes. +Various options within nip have been added or modified to take advantage of +these changes. The routine to translate DNA to protein and write the protein +to disk now gives correct results for spliced genes. The routine to translate +DNA to protein and display the two together now gives correct translations +except for the amino acids spanning intron/exon junctions. The routine to plot +maps from feature tables can use the new style. The open reading frame finding +routine writes out its results in the new style. The routine that finds open +reading frames and writes their translations to disk also writes a title in the +form of a new style feature table entry. The feature table format output from +the pattern searches in nip also uses the new style. + + + +---------------------------------------------------------------------------- + + Section VI Indexing the sequence libraries + -------------------------------------------- + +We handle EMBL, SwissProt, and GenBank in their distributed format, plus +PIR and NRL3D in codata format. All programs and scripts are in directory +indexseqlibs. + +Currently we produce entryname index, accession number index freetext index, +and brief index (brief index contains the entry name the primary accession +number the sequence length and an 80 character description). + +To produce any of the indexes requires the creation of several intermediate +files and the indexing programs are written so that the intermediate files +are the same for all libraries. This means that only the programs that read +the distributed form of each library need to be unique to that library, and +all the other processing programs can be used for all libraries. + + +However even the though the indexes have the same format, programs (like nip) +that read the libraries need to treat each library separately because their +actual contents are written differently. + +Making the entry name index +--------------------------- + +Common program entryname2 + +EMBL emblentryname1 +SwissProt emblentryname1 + +GenBank genbentryname1 + +PIR pirentryname1 +NRL3D pirentryname1 + + +Making the accession number index +--------------------------------- + +Common programs access2 access3 access4 + +EMBL emblaccess1 +SwissProt emblaccess1 + +GenBank genbaccess1 + +PIR piraccess1 piraccess2 +NRL3D No accession numbers + +Making the brief index +---------------------- + +Common program title2 + +EMBL embltitle1 +SwissProt embltitle1 + +GenBank genbtitle1 + +PIR pirtitle1 pirtitle2 (pir3 has no accession numbers) +NRL3D pirtitle2 + +Scripts +------- + +emblentryname.script +emblaccession.script +embltitle.script + +swissentryname.script +swissaccession.script +swisstitle.script + +genbentrynamescript +genbaccession.script +genbtitle.script + +pirentryname.script +piraccession.script +pirtitle.script + +nrl3dentryname.script +nrl3dtitle.script + + + + + + + + diff --git a/Staden_install-alpha b/Staden_install-alpha new file mode 100644 index 0000000..34a9b69 --- /dev/null +++ b/Staden_install-alpha @@ -0,0 +1,453 @@ +#! /bin/csh -f +# +# staden_install - version 2.4 +# +# This is a prototype installation program. +# +# 9 March 1992 +# Modified for installation on Sun, Alliant, etc +# No longer install 2rs +# +# 20 November 1992 +# Now includes convert, cop, frog, getMCH and scf +# +# 25 November 1992 +# SGI supported +# +# 19 May 1993 +# DEC Alpha, Solaris supported +# +# Written by sd@uk.ac.cam.mrc-lmb +# + +# prelim +set prog = $0 ; set prog = $prog:t + +# Machines supported: al sun dec sgi alpha solaris +#set MACHINE = `echo $prog | sed 's/.*-//'` +set MACHINE = alpha + +# For local (MRC-LMB) setup only +#set LOCAL = `echo $prog | awk '/local/{print "YES";exit;}{print "NO";}'` +set LOCAL = NO + + +echo "" +echo -n "Staden Package installation procedure - " +switch (${MACHINE}) + case "al": + echo "Alliant FX/2800 Concentrix version" + set MAKE = "make -sk" + breaksw + case "sun": + echo "SunOS version" + set MAKE = "make -sk" + breaksw + case "dec": + echo "DEC Ultrix (mips) version" + set MAKE = "gmake -sk" + breaksw + case "sgi": + echo "Silicon Graphics Iris version" + set MAKE = "gmake -sk" + breaksw + case "alpha": + echo "DEC Alpha OSF/1 version" + set MAKE = "gmake -sk" + breaksw + case "solaris": + echo "Solaris version" + set MAKE = "make -sk" + breaksw + default: + echo "Panic. Unknown version" + exit 1 +endsw +echo "" +echo "* starting initialization...please wait." +echo "" + +# Binary fork of source directory +if ($LOCAL == "YES") then + set DIR_BINARIES = ${MACHINE}-binaries + set DIR_PROGS = ${MACHINE}-bin +else + set DIR_BINARIES = . + set DIR_PROGS = bin + set MAKE = "$MAKE -f makefile-${MACHINE}" +endif + +init: +# Set useful shell variables +set YES="YES"; +set NO="NO" + +# set/unset some .cshrc envs. +unset noclobber +set noglob + +# set interrupt trap +onintr end_failure + +# Make dir command +set MKDIR = "mkdir" + +# Copy command +set CP = "cp -p" + +# Install command +#set INSTALL = "install" +#set INSTALL = "mv" +set INSTALL = "cp" + +# Set up default responses +set DEF_STADEN_ROOT = `pwd` + +set DEF_REQ_NONX = "$YES" +set DEF_REQ_X = "$YES" +set DEF_REQ_TED = "$YES" +set DEF_REQ_MISC = "$YES" + +# directories +set DIR_SRC = $DEF_STADEN_ROOT/src +set DIR_BIN = $DEF_STADEN_ROOT/$DIR_PROGS +set DIR_MISC = $DIR_SRC/Misc +set DIR_STADEN = $DIR_SRC/staden +set DIR_TED = $DIR_SRC/ted +set DIR_ABI = $DIR_SRC/abi +set DIR_ALF = $DIR_SRC/alf +set DIR_BAP = $DIR_SRC/bap +set DIR_OSP = $DIR_SRC/bap/osp-bits +set DIR_CONVERT = $DIR_SRC/convert +set DIR_COP = $DIR_SRC/cop +set DIR_FROG = $DIR_SRC/frog +set DIR_GETMCH = $DIR_SRC/getMCH +set DIR_SCF = $DIR_SRC/scf + + +main: + + +preamble: + echo "" + echo "" + echo "* Please answer the following questions." + echo " Default answers to questions are given in square brackets." + echo " If you require help at any stage respond with a ? to the question." + echo "" + +ask_staden_root: + set ANS_STADEN_ROOT = $DEF_STADEN_ROOT + +ask_require_nonx_progs: + echo -n "Compile all the non-X programs in the Staden Package [$DEF_REQ_NONX]? " + set ANS_REQ_NONX = $< + if ("$ANS_REQ_NONX" == "?") then + echo "* If you do not have X windows on your system you will require" + echo " these. However, you will require Tektronics terminal emulation." + echo " If you do not require all of the non-X programs, you should abort" + echo " and manually make the ones you require." + echo "" + goto ask_require_nonx_progs + else if ("$ANS_REQ_NONX" != "") then + if ("$ANS_REQ_NONX" =~ [yY]*) then + set ANS_REQ_NONX=$YES + else if ("$ANS_REQ_NONX" =~ [nN]*) then + set ANS_REQ_NONX=$NO + else + goto ask_require_nonx_progs + endif + else + set ANS_REQ_NONX=$DEF_REQ_NONX + endif + +ask_require_x_progs: + echo -n "Compile all the X programs in the Staden Package [$DEF_REQ_X]? " + set ANS_REQ_X = $< + if ("$ANS_REQ_X" == "?") then + echo "* These are the programs that require X windows." + echo " If you do not require all of the X programs, you should abort" + echo " and manually make the ones you require." + + echo "" + goto ask_require_x_progs + else if ("$ANS_REQ_X" != "") then + if ("$ANS_REQ_X" =~ [yY]*) then + set ANS_REQ_X=$YES + else if ("$ANS_REQ_X" =~ [nN]*) then + set ANS_REQ_X=$NO + else + goto ask_require_nonx_progs + endif + else + set ANS_REQ_X=$DEF_REQ_X + endif + + +ask_require_ted: + echo -n "Compile the trace editing program ted [$DEF_REQ_TED]? " + set ANS_REQ_TED = $< + if ("$ANS_REQ_TED" == "?") then + echo "* This is the trace editor program. It allows you to look at" + echo " traces obtained from automated fluorescent sequencing machines." + echo "" + goto ask_require_ted + else if ("$ANS_REQ_TED" != "") then + if ("$ANS_REQ_TED" =~ [yY]*) then + set ANS_REQ_TED=$YES + else if ("$ANS_REQ_TED" =~ [nN]*) then + set ANS_REQ_TED=$NO + else + goto ask_require_ted + endif + else + set ANS_REQ_TED=$DEF_REQ_TED + endif + + + +ask_require_misc: + echo -n "Compile other programs [$DEF_REQ_MISC]? " + set ANS_REQ_MISC = $< + if ("$ANS_REQ_MISC" == "?") then + echo "* Other programs include:" + echo " alfsplit" + echo " getABISampleName" + echo "" + goto ask_require_misc + else if ("$ANS_REQ_MISC" != "") then + if ("$ANS_REQ_MISC" =~ [yY]*) then + set ANS_REQ_MISC=$YES + else if ("$ANS_REQ_MISC" =~ [nN]*) then + set ANS_REQ_MISC=$NO + else + goto ask_require_misc + endif + else + set ANS_REQ_MISC=$DEF_REQ_MISC + endif + + + +time_taken_warning: + echo "" + echo "The installation procedure is now ready to start." + echo "" + echo "**** Warning:" + echo " The installation will take considerable time to complete. If you" + echo " are installing the whole Staden Package from scratch it could" + echo " take as long as an hour for all exectuables to be compiled and" + echo " installed." + echo "" + +ask_goahead: + echo -n "Proceed with the installation [YES]? " + set ANSWER=$< + if ("$ANSWER" == "?") then + echo "* Final confirmation to proceed with the installation. Answer" + echo " YES to proceed; otherwise, answer NO to abort the installation." + echo "" + goto ask_goahead + else if ("$ANSWER" != "") then + if ("$ANSWER" =~ [nN]*) then + goto chickens_exit + else if ("$ANSWER" !~ [yY]*) then + goto ask_goahead + endif + endif + +installation_proper: + +# make binaries directory if it doesn't exist + + if (! -d $DIR_BIN) then + $MKDIR $DIR_BIN + endif + + if ("$ANS_REQ_MISC" == "$YES" || "$ANS_REQ_X" == "$YES" || "$ANS_REQ_NONX" == "$YES" ) then + echo "" + echo "+ Compiling miscellaneous library" + + pushd $DIR_MISC > /dev/null + + cd $DIR_BINARIES + $MAKE all + + popd > /dev/null + + endif + + if ("$ANS_REQ_NONX" == "$YES") then + echo "" + echo "+ Installing non X programs" + + pushd $DIR_STADEN > /dev/null + cd $DIR_BINARIES + $MAKE nprogs lprogs + $INSTALL mep $DIR_BIN + $INSTALL nip $DIR_BIN + $INSTALL pip $DIR_BIN + $INSTALL sap $DIR_BIN + $INSTALL sapf $DIR_BIN + $INSTALL sip $DIR_BIN + $INSTALL splitp1 $DIR_BIN + $INSTALL splitp2 $DIR_BIN + $INSTALL splitp3 $DIR_BIN + $INSTALL sethelp $DIR_BIN + $INSTALL gip $DIR_BIN + $INSTALL nipl $DIR_BIN + $INSTALL pipl $DIR_BIN + $INSTALL sipl $DIR_BIN + $INSTALL dap $DIR_BIN + $INSTALL nipf $DIR_BIN + $INSTALL vep $DIR_BIN + $INSTALL rep $DIR_BIN + $INSTALL lip $DIR_BIN + #$INSTALL convert_project $DIR_BIN + popd > /dev/null + + pushd $DIR_OSP > /dev/null + cd $DIR_BINARIES + $MAKE + popd > /dev/null + + pushd $DIR_BAP > /dev/null + cd $DIR_BINARIES + $MAKE bap + $INSTALL bap $DIR_BIN + popd > /dev/null + + endif + + if ("$ANS_REQ_TED" == "$YES") then + echo "" + echo "+ Installing Trace editor" + + pushd $DIR_TED > /dev/null + cd $DIR_BINARIES + $MAKE ted + $INSTALL ted $DIR_BIN + popd > /dev/null + endif + + if ("$ANS_REQ_X" == "$YES") then + echo "" + echo "+ Installing X programs" + + pushd $DIR_STADEN > /dev/null + cd $DIR_BINARIES + $MAKE xprogs + $INSTALL xmep $DIR_BIN + $INSTALL xnip $DIR_BIN + $INSTALL xpip $DIR_BIN + $INSTALL xsap $DIR_BIN + $INSTALL xsip $DIR_BIN + $INSTALL xdap $DIR_BIN + popd > /dev/null + + pushd $DIR_OSP > /dev/null + cd $DIR_BINARIES + $MAKE + popd > /dev/null + + pushd $DIR_BAP > /dev/null + cd $DIR_BINARIES + $MAKE xbap + $INSTALL xbap $DIR_BIN + popd > /dev/null + + + endif + + if ("$ANS_REQ_MISC" == "$YES") then + echo "" + echo "+ Installing miscellaneous programs" + + pushd $DIR_ABI > /dev/null + cd $DIR_BINARIES + $MAKE all + $INSTALL getABISampleName $DIR_BIN + popd > /dev/null + + pushd $DIR_ALF > /dev/null + cd $DIR_BINARIES + $MAKE alfsplit + $INSTALL alfsplit $DIR_BIN + popd > /dev/null + + pushd $DIR_CONVERT > /dev/null + cd $DIR_BINARIES + $MAKE convert + $INSTALL convert $DIR_BIN + popd > /dev/null + + pushd $DIR_COP > /dev/null + cd $DIR_BINARIES + $MAKE all + $INSTALL cop $DIR_BIN + $INSTALL cop-bap $DIR_BIN + popd > /dev/null + + pushd $DIR_FROG > /dev/null + cd $DIR_BINARIES + $MAKE frog + $INSTALL frog $DIR_BIN + popd > /dev/null + + pushd $DIR_GETMCH > /dev/null + cd $DIR_BINARIES + $MAKE trace2seq + $INSTALL trace2seq $DIR_BIN + popd > /dev/null + + pushd $DIR_SCF > /dev/null + cd $DIR_BINARIES + $MAKE makeSCF + $INSTALL makeSCF $DIR_BIN + popd > /dev/null + + + + endif + + +installation_done: + echo "" + echo "+ Installation completed" + echo "" + + echo " Some further initialisation is required in order to use the" + echo " package. csh users should insert the following in their .login" + echo " files:" + echo " " + echo " setenv STADENROOT $ANS_STADEN_ROOT" + echo ' source $STADENROOT/staden.login' + echo " " + echo " Users of the Bourne shell, sh, should insert the following in" + echo " their .profile:" + echo " " + echo " STADENROOT=$ANS_STADEN_ROOT" + echo " export STADENROOT" + echo ' . $STADENROOT/staden.profile' + echo " " + echo " These initialisations will alter the shell's search path so that" + echo " it can find the programs in the STADEN Package" + echo " " + +normal_exit: + exit 0 + +chickens_exit: + echo "" + echo "+ Installation cancelled" + echo "" + + exit 0 + +end_failure: + unset noglob + echo "" + echo "Aborted STADEN Package installation on `date`" + echo "" + exit 1 + diff --git a/Staden_install-dec b/Staden_install-dec new file mode 100644 index 0000000..9b06240 --- /dev/null +++ b/Staden_install-dec @@ -0,0 +1,453 @@ +#! /bin/csh -f +# +# staden_install - version 2.4 +# +# This is a prototype installation program. +# +# 9 March 1992 +# Modified for installation on Sun, Alliant, etc +# No longer install 2rs +# +# 20 November 1992 +# Now includes convert, cop, frog, getMCH and scf +# +# 25 November 1992 +# SGI supported +# +# 19 May 1993 +# DEC Alpha, Solaris supported +# +# Written by sd@uk.ac.cam.mrc-lmb +# + +# prelim +set prog = $0 ; set prog = $prog:t + +# Machines supported: al sun dec sgi alpha solaris +#set MACHINE = `echo $prog | sed 's/.*-//'` +set MACHINE = dec + +# For local (MRC-LMB) setup only +#set LOCAL = `echo $prog | awk '/local/{print "YES";exit;}{print "NO";}'` +set LOCAL = NO + + +echo "" +echo -n "Staden Package installation procedure - " +switch (${MACHINE}) + case "al": + echo "Alliant FX/2800 Concentrix version" + set MAKE = "make -sk" + breaksw + case "sun": + echo "SunOS version" + set MAKE = "make -sk" + breaksw + case "dec": + echo "DEC Ultrix (mips) version" + set MAKE = "gmake -sk" + breaksw + case "sgi": + echo "Silicon Graphics Iris version" + set MAKE = "gmake -sk" + breaksw + case "alpha": + echo "DEC Alpha OSF/1 version" + set MAKE = "gmake -sk" + breaksw + case "solaris": + echo "Solaris version" + set MAKE = "make -sk" + breaksw + default: + echo "Panic. Unknown version" + exit 1 +endsw +echo "" +echo "* starting initialization...please wait." +echo "" + +# Binary fork of source directory +if ($LOCAL == "YES") then + set DIR_BINARIES = ${MACHINE}-binaries + set DIR_PROGS = ${MACHINE}-bin +else + set DIR_BINARIES = . + set DIR_PROGS = bin + set MAKE = "$MAKE -f makefile-${MACHINE}" +endif + +init: +# Set useful shell variables +set YES="YES"; +set NO="NO" + +# set/unset some .cshrc envs. +unset noclobber +set noglob + +# set interrupt trap +onintr end_failure + +# Make dir command +set MKDIR = "mkdir" + +# Copy command +set CP = "cp -p" + +# Install command +#set INSTALL = "install" +#set INSTALL = "mv" +set INSTALL = "cp" + +# Set up default responses +set DEF_STADEN_ROOT = `pwd` + +set DEF_REQ_NONX = "$YES" +set DEF_REQ_X = "$YES" +set DEF_REQ_TED = "$YES" +set DEF_REQ_MISC = "$YES" + +# directories +set DIR_SRC = $DEF_STADEN_ROOT/src +set DIR_BIN = $DEF_STADEN_ROOT/$DIR_PROGS +set DIR_MISC = $DIR_SRC/Misc +set DIR_STADEN = $DIR_SRC/staden +set DIR_TED = $DIR_SRC/ted +set DIR_ABI = $DIR_SRC/abi +set DIR_ALF = $DIR_SRC/alf +set DIR_BAP = $DIR_SRC/bap +set DIR_OSP = $DIR_SRC/bap/osp-bits +set DIR_CONVERT = $DIR_SRC/convert +set DIR_COP = $DIR_SRC/cop +set DIR_FROG = $DIR_SRC/frog +set DIR_GETMCH = $DIR_SRC/getMCH +set DIR_SCF = $DIR_SRC/scf + + +main: + + +preamble: + echo "" + echo "" + echo "* Please answer the following questions." + echo " Default answers to questions are given in square brackets." + echo " If you require help at any stage respond with a ? to the question." + echo "" + +ask_staden_root: + set ANS_STADEN_ROOT = $DEF_STADEN_ROOT + +ask_require_nonx_progs: + echo -n "Compile all the non-X programs in the Staden Package [$DEF_REQ_NONX]? " + set ANS_REQ_NONX = $< + if ("$ANS_REQ_NONX" == "?") then + echo "* If you do not have X windows on your system you will require" + echo " these. However, you will require Tektronics terminal emulation." + echo " If you do not require all of the non-X programs, you should abort" + echo " and manually make the ones you require." + echo "" + goto ask_require_nonx_progs + else if ("$ANS_REQ_NONX" != "") then + if ("$ANS_REQ_NONX" =~ [yY]*) then + set ANS_REQ_NONX=$YES + else if ("$ANS_REQ_NONX" =~ [nN]*) then + set ANS_REQ_NONX=$NO + else + goto ask_require_nonx_progs + endif + else + set ANS_REQ_NONX=$DEF_REQ_NONX + endif + +ask_require_x_progs: + echo -n "Compile all the X programs in the Staden Package [$DEF_REQ_X]? " + set ANS_REQ_X = $< + if ("$ANS_REQ_X" == "?") then + echo "* These are the programs that require X windows." + echo " If you do not require all of the X programs, you should abort" + echo " and manually make the ones you require." + + echo "" + goto ask_require_x_progs + else if ("$ANS_REQ_X" != "") then + if ("$ANS_REQ_X" =~ [yY]*) then + set ANS_REQ_X=$YES + else if ("$ANS_REQ_X" =~ [nN]*) then + set ANS_REQ_X=$NO + else + goto ask_require_nonx_progs + endif + else + set ANS_REQ_X=$DEF_REQ_X + endif + + +ask_require_ted: + echo -n "Compile the trace editing program ted [$DEF_REQ_TED]? " + set ANS_REQ_TED = $< + if ("$ANS_REQ_TED" == "?") then + echo "* This is the trace editor program. It allows you to look at" + echo " traces obtained from automated fluorescent sequencing machines." + echo "" + goto ask_require_ted + else if ("$ANS_REQ_TED" != "") then + if ("$ANS_REQ_TED" =~ [yY]*) then + set ANS_REQ_TED=$YES + else if ("$ANS_REQ_TED" =~ [nN]*) then + set ANS_REQ_TED=$NO + else + goto ask_require_ted + endif + else + set ANS_REQ_TED=$DEF_REQ_TED + endif + + + +ask_require_misc: + echo -n "Compile other programs [$DEF_REQ_MISC]? " + set ANS_REQ_MISC = $< + if ("$ANS_REQ_MISC" == "?") then + echo "* Other programs include:" + echo " alfsplit" + echo " getABISampleName" + echo "" + goto ask_require_misc + else if ("$ANS_REQ_MISC" != "") then + if ("$ANS_REQ_MISC" =~ [yY]*) then + set ANS_REQ_MISC=$YES + else if ("$ANS_REQ_MISC" =~ [nN]*) then + set ANS_REQ_MISC=$NO + else + goto ask_require_misc + endif + else + set ANS_REQ_MISC=$DEF_REQ_MISC + endif + + + +time_taken_warning: + echo "" + echo "The installation procedure is now ready to start." + echo "" + echo "**** Warning:" + echo " The installation will take considerable time to complete. If you" + echo " are installing the whole Staden Package from scratch it could" + echo " take as long as an hour for all exectuables to be compiled and" + echo " installed." + echo "" + +ask_goahead: + echo -n "Proceed with the installation [YES]? " + set ANSWER=$< + if ("$ANSWER" == "?") then + echo "* Final confirmation to proceed with the installation. Answer" + echo " YES to proceed; otherwise, answer NO to abort the installation." + echo "" + goto ask_goahead + else if ("$ANSWER" != "") then + if ("$ANSWER" =~ [nN]*) then + goto chickens_exit + else if ("$ANSWER" !~ [yY]*) then + goto ask_goahead + endif + endif + +installation_proper: + +# make binaries directory if it doesn't exist + + if (! -d $DIR_BIN) then + $MKDIR $DIR_BIN + endif + + if ("$ANS_REQ_MISC" == "$YES" || "$ANS_REQ_X" == "$YES" || "$ANS_REQ_NONX" == "$YES" ) then + echo "" + echo "+ Compiling miscellaneous library" + + pushd $DIR_MISC > /dev/null + + cd $DIR_BINARIES + $MAKE all + + popd > /dev/null + + endif + + if ("$ANS_REQ_NONX" == "$YES") then + echo "" + echo "+ Installing non X programs" + + pushd $DIR_STADEN > /dev/null + cd $DIR_BINARIES + $MAKE nprogs lprogs + $INSTALL mep $DIR_BIN + $INSTALL nip $DIR_BIN + $INSTALL pip $DIR_BIN + $INSTALL sap $DIR_BIN + $INSTALL sapf $DIR_BIN + $INSTALL sip $DIR_BIN + $INSTALL splitp1 $DIR_BIN + $INSTALL splitp2 $DIR_BIN + $INSTALL splitp3 $DIR_BIN + $INSTALL sethelp $DIR_BIN + $INSTALL gip $DIR_BIN + $INSTALL nipl $DIR_BIN + $INSTALL pipl $DIR_BIN + $INSTALL sipl $DIR_BIN + $INSTALL dap $DIR_BIN + $INSTALL nipf $DIR_BIN + $INSTALL vep $DIR_BIN + $INSTALL rep $DIR_BIN + $INSTALL lip $DIR_BIN + #$INSTALL convert_project $DIR_BIN + popd > /dev/null + + pushd $DIR_OSP > /dev/null + cd $DIR_BINARIES + $MAKE + popd > /dev/null + + pushd $DIR_BAP > /dev/null + cd $DIR_BINARIES + $MAKE bap + $INSTALL bap $DIR_BIN + popd > /dev/null + + endif + + if ("$ANS_REQ_TED" == "$YES") then + echo "" + echo "+ Installing Trace editor" + + pushd $DIR_TED > /dev/null + cd $DIR_BINARIES + $MAKE ted + $INSTALL ted $DIR_BIN + popd > /dev/null + endif + + if ("$ANS_REQ_X" == "$YES") then + echo "" + echo "+ Installing X programs" + + pushd $DIR_STADEN > /dev/null + cd $DIR_BINARIES + $MAKE xprogs + $INSTALL xmep $DIR_BIN + $INSTALL xnip $DIR_BIN + $INSTALL xpip $DIR_BIN + $INSTALL xsap $DIR_BIN + $INSTALL xsip $DIR_BIN + $INSTALL xdap $DIR_BIN + popd > /dev/null + + pushd $DIR_OSP > /dev/null + cd $DIR_BINARIES + $MAKE + popd > /dev/null + + pushd $DIR_BAP > /dev/null + cd $DIR_BINARIES + $MAKE xbap + $INSTALL xbap $DIR_BIN + popd > /dev/null + + + endif + + if ("$ANS_REQ_MISC" == "$YES") then + echo "" + echo "+ Installing miscellaneous programs" + + pushd $DIR_ABI > /dev/null + cd $DIR_BINARIES + $MAKE all + $INSTALL getABISampleName $DIR_BIN + popd > /dev/null + + pushd $DIR_ALF > /dev/null + cd $DIR_BINARIES + $MAKE alfsplit + $INSTALL alfsplit $DIR_BIN + popd > /dev/null + + pushd $DIR_CONVERT > /dev/null + cd $DIR_BINARIES + $MAKE convert + $INSTALL convert $DIR_BIN + popd > /dev/null + + pushd $DIR_COP > /dev/null + cd $DIR_BINARIES + $MAKE all + $INSTALL cop $DIR_BIN + $INSTALL cop-bap $DIR_BIN + popd > /dev/null + + pushd $DIR_FROG > /dev/null + cd $DIR_BINARIES + $MAKE frog + $INSTALL frog $DIR_BIN + popd > /dev/null + + pushd $DIR_GETMCH > /dev/null + cd $DIR_BINARIES + $MAKE trace2seq + $INSTALL trace2seq $DIR_BIN + popd > /dev/null + + pushd $DIR_SCF > /dev/null + cd $DIR_BINARIES + $MAKE makeSCF + $INSTALL makeSCF $DIR_BIN + popd > /dev/null + + + + endif + + +installation_done: + echo "" + echo "+ Installation completed" + echo "" + + echo " Some further initialisation is required in order to use the" + echo " package. csh users should insert the following in their .login" + echo " files:" + echo " " + echo " setenv STADENROOT $ANS_STADEN_ROOT" + echo ' source $STADENROOT/staden.login' + echo " " + echo " Users of the Bourne shell, sh, should insert the following in" + echo " their .profile:" + echo " " + echo " STADENROOT=$ANS_STADEN_ROOT" + echo " export STADENROOT" + echo ' . $STADENROOT/staden.profile' + echo " " + echo " These initialisations will alter the shell's search path so that" + echo " it can find the programs in the STADEN Package" + echo " " + +normal_exit: + exit 0 + +chickens_exit: + echo "" + echo "+ Installation cancelled" + echo "" + + exit 0 + +end_failure: + unset noglob + echo "" + echo "Aborted STADEN Package installation on `date`" + echo "" + exit 1 + diff --git a/Staden_install-sgi b/Staden_install-sgi new file mode 100644 index 0000000..7ce5b1e --- /dev/null +++ b/Staden_install-sgi @@ -0,0 +1,453 @@ +#! /bin/csh -f +# +# staden_install - version 2.4 +# +# This is a prototype installation program. +# +# 9 March 1992 +# Modified for installation on Sun, Alliant, etc +# No longer install 2rs +# +# 20 November 1992 +# Now includes convert, cop, frog, getMCH and scf +# +# 25 November 1992 +# SGI supported +# +# 19 May 1993 +# DEC Alpha, Solaris supported +# +# Written by sd@uk.ac.cam.mrc-lmb +# + +# prelim +set prog = $0 ; set prog = $prog:t + +# Machines supported: al sun dec sgi alpha solaris +#set MACHINE = `echo $prog | sed 's/.*-//'` +set MACHINE = sgi + +# For local (MRC-LMB) setup only +#set LOCAL = `echo $prog | awk '/local/{print "YES";exit;}{print "NO";}'` +set LOCAL = NO + + +echo "" +echo -n "Staden Package installation procedure - " +switch (${MACHINE}) + case "al": + echo "Alliant FX/2800 Concentrix version" + set MAKE = "make -sk" + breaksw + case "sun": + echo "SunOS version" + set MAKE = "make -sk" + breaksw + case "dec": + echo "DEC Ultrix (mips) version" + set MAKE = "gmake -sk" + breaksw + case "sgi": + echo "Silicon Graphics Iris version" + set MAKE = "gmake -sk" + breaksw + case "alpha": + echo "DEC Alpha OSF/1 version" + set MAKE = "gmake -sk" + breaksw + case "solaris": + echo "Solaris version" + set MAKE = "make -sk" + breaksw + default: + echo "Panic. Unknown version" + exit 1 +endsw +echo "" +echo "* starting initialization...please wait." +echo "" + +# Binary fork of source directory +if ($LOCAL == "YES") then + set DIR_BINARIES = ${MACHINE}-binaries + set DIR_PROGS = ${MACHINE}-bin +else + set DIR_BINARIES = . + set DIR_PROGS = bin + set MAKE = "$MAKE -f makefile-${MACHINE}" +endif + +init: +# Set useful shell variables +set YES="YES"; +set NO="NO" + +# set/unset some .cshrc envs. +unset noclobber +set noglob + +# set interrupt trap +onintr end_failure + +# Make dir command +set MKDIR = "mkdir" + +# Copy command +set CP = "cp -p" + +# Install command +#set INSTALL = "install" +#set INSTALL = "mv" +set INSTALL = "cp" + +# Set up default responses +set DEF_STADEN_ROOT = `pwd` + +set DEF_REQ_NONX = "$YES" +set DEF_REQ_X = "$YES" +set DEF_REQ_TED = "$YES" +set DEF_REQ_MISC = "$YES" + +# directories +set DIR_SRC = $DEF_STADEN_ROOT/src +set DIR_BIN = $DEF_STADEN_ROOT/$DIR_PROGS +set DIR_MISC = $DIR_SRC/Misc +set DIR_STADEN = $DIR_SRC/staden +set DIR_TED = $DIR_SRC/ted +set DIR_ABI = $DIR_SRC/abi +set DIR_ALF = $DIR_SRC/alf +set DIR_BAP = $DIR_SRC/bap +set DIR_OSP = $DIR_SRC/bap/osp-bits +set DIR_CONVERT = $DIR_SRC/convert +set DIR_COP = $DIR_SRC/cop +set DIR_FROG = $DIR_SRC/frog +set DIR_GETMCH = $DIR_SRC/getMCH +set DIR_SCF = $DIR_SRC/scf + + +main: + + +preamble: + echo "" + echo "" + echo "* Please answer the following questions." + echo " Default answers to questions are given in square brackets." + echo " If you require help at any stage respond with a ? to the question." + echo "" + +ask_staden_root: + set ANS_STADEN_ROOT = $DEF_STADEN_ROOT + +ask_require_nonx_progs: + echo -n "Compile all the non-X programs in the Staden Package [$DEF_REQ_NONX]? " + set ANS_REQ_NONX = $< + if ("$ANS_REQ_NONX" == "?") then + echo "* If you do not have X windows on your system you will require" + echo " these. However, you will require Tektronics terminal emulation." + echo " If you do not require all of the non-X programs, you should abort" + echo " and manually make the ones you require." + echo "" + goto ask_require_nonx_progs + else if ("$ANS_REQ_NONX" != "") then + if ("$ANS_REQ_NONX" =~ [yY]*) then + set ANS_REQ_NONX=$YES + else if ("$ANS_REQ_NONX" =~ [nN]*) then + set ANS_REQ_NONX=$NO + else + goto ask_require_nonx_progs + endif + else + set ANS_REQ_NONX=$DEF_REQ_NONX + endif + +ask_require_x_progs: + echo -n "Compile all the X programs in the Staden Package [$DEF_REQ_X]? " + set ANS_REQ_X = $< + if ("$ANS_REQ_X" == "?") then + echo "* These are the programs that require X windows." + echo " If you do not require all of the X programs, you should abort" + echo " and manually make the ones you require." + + echo "" + goto ask_require_x_progs + else if ("$ANS_REQ_X" != "") then + if ("$ANS_REQ_X" =~ [yY]*) then + set ANS_REQ_X=$YES + else if ("$ANS_REQ_X" =~ [nN]*) then + set ANS_REQ_X=$NO + else + goto ask_require_nonx_progs + endif + else + set ANS_REQ_X=$DEF_REQ_X + endif + + +ask_require_ted: + echo -n "Compile the trace editing program ted [$DEF_REQ_TED]? " + set ANS_REQ_TED = $< + if ("$ANS_REQ_TED" == "?") then + echo "* This is the trace editor program. It allows you to look at" + echo " traces obtained from automated fluorescent sequencing machines." + echo "" + goto ask_require_ted + else if ("$ANS_REQ_TED" != "") then + if ("$ANS_REQ_TED" =~ [yY]*) then + set ANS_REQ_TED=$YES + else if ("$ANS_REQ_TED" =~ [nN]*) then + set ANS_REQ_TED=$NO + else + goto ask_require_ted + endif + else + set ANS_REQ_TED=$DEF_REQ_TED + endif + + + +ask_require_misc: + echo -n "Compile other programs [$DEF_REQ_MISC]? " + set ANS_REQ_MISC = $< + if ("$ANS_REQ_MISC" == "?") then + echo "* Other programs include:" + echo " alfsplit" + echo " getABISampleName" + echo "" + goto ask_require_misc + else if ("$ANS_REQ_MISC" != "") then + if ("$ANS_REQ_MISC" =~ [yY]*) then + set ANS_REQ_MISC=$YES + else if ("$ANS_REQ_MISC" =~ [nN]*) then + set ANS_REQ_MISC=$NO + else + goto ask_require_misc + endif + else + set ANS_REQ_MISC=$DEF_REQ_MISC + endif + + + +time_taken_warning: + echo "" + echo "The installation procedure is now ready to start." + echo "" + echo "**** Warning:" + echo " The installation will take considerable time to complete. If you" + echo " are installing the whole Staden Package from scratch it could" + echo " take as long as an hour for all exectuables to be compiled and" + echo " installed." + echo "" + +ask_goahead: + echo -n "Proceed with the installation [YES]? " + set ANSWER=$< + if ("$ANSWER" == "?") then + echo "* Final confirmation to proceed with the installation. Answer" + echo " YES to proceed; otherwise, answer NO to abort the installation." + echo "" + goto ask_goahead + else if ("$ANSWER" != "") then + if ("$ANSWER" =~ [nN]*) then + goto chickens_exit + else if ("$ANSWER" !~ [yY]*) then + goto ask_goahead + endif + endif + +installation_proper: + +# make binaries directory if it doesn't exist + + if (! -d $DIR_BIN) then + $MKDIR $DIR_BIN + endif + + if ("$ANS_REQ_MISC" == "$YES" || "$ANS_REQ_X" == "$YES" || "$ANS_REQ_NONX" == "$YES" ) then + echo "" + echo "+ Compiling miscellaneous library" + + pushd $DIR_MISC > /dev/null + + cd $DIR_BINARIES + $MAKE all + + popd > /dev/null + + endif + + if ("$ANS_REQ_NONX" == "$YES") then + echo "" + echo "+ Installing non X programs" + + pushd $DIR_STADEN > /dev/null + cd $DIR_BINARIES + $MAKE nprogs lprogs + $INSTALL mep $DIR_BIN + $INSTALL nip $DIR_BIN + $INSTALL pip $DIR_BIN + $INSTALL sap $DIR_BIN + $INSTALL sapf $DIR_BIN + $INSTALL sip $DIR_BIN + $INSTALL splitp1 $DIR_BIN + $INSTALL splitp2 $DIR_BIN + $INSTALL splitp3 $DIR_BIN + $INSTALL sethelp $DIR_BIN + $INSTALL gip $DIR_BIN + $INSTALL nipl $DIR_BIN + $INSTALL pipl $DIR_BIN + $INSTALL sipl $DIR_BIN + $INSTALL dap $DIR_BIN + $INSTALL nipf $DIR_BIN + $INSTALL vep $DIR_BIN + $INSTALL rep $DIR_BIN + $INSTALL lip $DIR_BIN + #$INSTALL convert_project $DIR_BIN + popd > /dev/null + + pushd $DIR_OSP > /dev/null + cd $DIR_BINARIES + $MAKE + popd > /dev/null + + pushd $DIR_BAP > /dev/null + cd $DIR_BINARIES + $MAKE bap + $INSTALL bap $DIR_BIN + popd > /dev/null + + endif + + if ("$ANS_REQ_TED" == "$YES") then + echo "" + echo "+ Installing Trace editor" + + pushd $DIR_TED > /dev/null + cd $DIR_BINARIES + $MAKE ted + $INSTALL ted $DIR_BIN + popd > /dev/null + endif + + if ("$ANS_REQ_X" == "$YES") then + echo "" + echo "+ Installing X programs" + + pushd $DIR_STADEN > /dev/null + cd $DIR_BINARIES + $MAKE xprogs + $INSTALL xmep $DIR_BIN + $INSTALL xnip $DIR_BIN + $INSTALL xpip $DIR_BIN + $INSTALL xsap $DIR_BIN + $INSTALL xsip $DIR_BIN + $INSTALL xdap $DIR_BIN + popd > /dev/null + + pushd $DIR_OSP > /dev/null + cd $DIR_BINARIES + $MAKE + popd > /dev/null + + pushd $DIR_BAP > /dev/null + cd $DIR_BINARIES + $MAKE xbap + $INSTALL xbap $DIR_BIN + popd > /dev/null + + + endif + + if ("$ANS_REQ_MISC" == "$YES") then + echo "" + echo "+ Installing miscellaneous programs" + + pushd $DIR_ABI > /dev/null + cd $DIR_BINARIES + $MAKE all + $INSTALL getABISampleName $DIR_BIN + popd > /dev/null + + pushd $DIR_ALF > /dev/null + cd $DIR_BINARIES + $MAKE alfsplit + $INSTALL alfsplit $DIR_BIN + popd > /dev/null + + pushd $DIR_CONVERT > /dev/null + cd $DIR_BINARIES + $MAKE convert + $INSTALL convert $DIR_BIN + popd > /dev/null + + pushd $DIR_COP > /dev/null + cd $DIR_BINARIES + $MAKE all + $INSTALL cop $DIR_BIN + $INSTALL cop-bap $DIR_BIN + popd > /dev/null + + pushd $DIR_FROG > /dev/null + cd $DIR_BINARIES + $MAKE frog + $INSTALL frog $DIR_BIN + popd > /dev/null + + pushd $DIR_GETMCH > /dev/null + cd $DIR_BINARIES + $MAKE trace2seq + $INSTALL trace2seq $DIR_BIN + popd > /dev/null + + pushd $DIR_SCF > /dev/null + cd $DIR_BINARIES + $MAKE makeSCF + $INSTALL makeSCF $DIR_BIN + popd > /dev/null + + + + endif + + +installation_done: + echo "" + echo "+ Installation completed" + echo "" + + echo " Some further initialisation is required in order to use the" + echo " package. csh users should insert the following in their .login" + echo " files:" + echo " " + echo " setenv STADENROOT $ANS_STADEN_ROOT" + echo ' source $STADENROOT/staden.login' + echo " " + echo " Users of the Bourne shell, sh, should insert the following in" + echo " their .profile:" + echo " " + echo " STADENROOT=$ANS_STADEN_ROOT" + echo " export STADENROOT" + echo ' . $STADENROOT/staden.profile' + echo " " + echo " These initialisations will alter the shell's search path so that" + echo " it can find the programs in the STADEN Package" + echo " " + +normal_exit: + exit 0 + +chickens_exit: + echo "" + echo "+ Installation cancelled" + echo "" + + exit 0 + +end_failure: + unset noglob + echo "" + echo "Aborted STADEN Package installation on `date`" + echo "" + exit 1 + diff --git a/Staden_install-solaris b/Staden_install-solaris new file mode 100644 index 0000000..93c2feb --- /dev/null +++ b/Staden_install-solaris @@ -0,0 +1,453 @@ +#! /bin/csh -f +# +# staden_install - version 2.4 +# +# This is a prototype installation program. +# +# 9 March 1992 +# Modified for installation on Sun, Alliant, etc +# No longer install 2rs +# +# 20 November 1992 +# Now includes convert, cop, frog, getMCH and scf +# +# 25 November 1992 +# SGI supported +# +# 19 May 1993 +# DEC Alpha, Solaris supported +# +# Written by sd@uk.ac.cam.mrc-lmb +# + +# prelim +set prog = $0 ; set prog = $prog:t + +# Machines supported: al sun dec sgi alpha solaris +#set MACHINE = `echo $prog | sed 's/.*-//'` +set MACHINE = solaris + +# For local (MRC-LMB) setup only +#set LOCAL = `echo $prog | awk '/local/{print "YES";exit;}{print "NO";}'` +set LOCAL = NO + + +echo "" +echo -n "Staden Package installation procedure - " +switch (${MACHINE}) + case "al": + echo "Alliant FX/2800 Concentrix version" + set MAKE = "make -sk" + breaksw + case "sun": + echo "SunOS version" + set MAKE = "make -sk" + breaksw + case "dec": + echo "DEC Ultrix (mips) version" + set MAKE = "gmake -sk" + breaksw + case "sgi": + echo "Silicon Graphics Iris version" + set MAKE = "gmake -sk" + breaksw + case "alpha": + echo "DEC Alpha OSF/1 version" + set MAKE = "gmake -sk" + breaksw + case "solaris": + echo "Solaris version" + set MAKE = "make -sk" + breaksw + default: + echo "Panic. Unknown version" + exit 1 +endsw +echo "" +echo "* starting initialization...please wait." +echo "" + +# Binary fork of source directory +if ($LOCAL == "YES") then + set DIR_BINARIES = ${MACHINE}-binaries + set DIR_PROGS = ${MACHINE}-bin +else + set DIR_BINARIES = . + set DIR_PROGS = bin + set MAKE = "$MAKE -f makefile-${MACHINE}" +endif + +init: +# Set useful shell variables +set YES="YES"; +set NO="NO" + +# set/unset some .cshrc envs. +unset noclobber +set noglob + +# set interrupt trap +onintr end_failure + +# Make dir command +set MKDIR = "mkdir" + +# Copy command +set CP = "cp -p" + +# Install command +#set INSTALL = "install" +#set INSTALL = "mv" +set INSTALL = "cp" + +# Set up default responses +set DEF_STADEN_ROOT = `pwd` + +set DEF_REQ_NONX = "$YES" +set DEF_REQ_X = "$YES" +set DEF_REQ_TED = "$YES" +set DEF_REQ_MISC = "$YES" + +# directories +set DIR_SRC = $DEF_STADEN_ROOT/src +set DIR_BIN = $DEF_STADEN_ROOT/$DIR_PROGS +set DIR_MISC = $DIR_SRC/Misc +set DIR_STADEN = $DIR_SRC/staden +set DIR_TED = $DIR_SRC/ted +set DIR_ABI = $DIR_SRC/abi +set DIR_ALF = $DIR_SRC/alf +set DIR_BAP = $DIR_SRC/bap +set DIR_OSP = $DIR_SRC/bap/osp-bits +set DIR_CONVERT = $DIR_SRC/convert +set DIR_COP = $DIR_SRC/cop +set DIR_FROG = $DIR_SRC/frog +set DIR_GETMCH = $DIR_SRC/getMCH +set DIR_SCF = $DIR_SRC/scf + + +main: + + +preamble: + echo "" + echo "" + echo "* Please answer the following questions." + echo " Default answers to questions are given in square brackets." + echo " If you require help at any stage respond with a ? to the question." + echo "" + +ask_staden_root: + set ANS_STADEN_ROOT = $DEF_STADEN_ROOT + +ask_require_nonx_progs: + echo -n "Compile all the non-X programs in the Staden Package [$DEF_REQ_NONX]? " + set ANS_REQ_NONX = $< + if ("$ANS_REQ_NONX" == "?") then + echo "* If you do not have X windows on your system you will require" + echo " these. However, you will require Tektronics terminal emulation." + echo " If you do not require all of the non-X programs, you should abort" + echo " and manually make the ones you require." + echo "" + goto ask_require_nonx_progs + else if ("$ANS_REQ_NONX" != "") then + if ("$ANS_REQ_NONX" =~ [yY]*) then + set ANS_REQ_NONX=$YES + else if ("$ANS_REQ_NONX" =~ [nN]*) then + set ANS_REQ_NONX=$NO + else + goto ask_require_nonx_progs + endif + else + set ANS_REQ_NONX=$DEF_REQ_NONX + endif + +ask_require_x_progs: + echo -n "Compile all the X programs in the Staden Package [$DEF_REQ_X]? " + set ANS_REQ_X = $< + if ("$ANS_REQ_X" == "?") then + echo "* These are the programs that require X windows." + echo " If you do not require all of the X programs, you should abort" + echo " and manually make the ones you require." + + echo "" + goto ask_require_x_progs + else if ("$ANS_REQ_X" != "") then + if ("$ANS_REQ_X" =~ [yY]*) then + set ANS_REQ_X=$YES + else if ("$ANS_REQ_X" =~ [nN]*) then + set ANS_REQ_X=$NO + else + goto ask_require_nonx_progs + endif + else + set ANS_REQ_X=$DEF_REQ_X + endif + + +ask_require_ted: + echo -n "Compile the trace editing program ted [$DEF_REQ_TED]? " + set ANS_REQ_TED = $< + if ("$ANS_REQ_TED" == "?") then + echo "* This is the trace editor program. It allows you to look at" + echo " traces obtained from automated fluorescent sequencing machines." + echo "" + goto ask_require_ted + else if ("$ANS_REQ_TED" != "") then + if ("$ANS_REQ_TED" =~ [yY]*) then + set ANS_REQ_TED=$YES + else if ("$ANS_REQ_TED" =~ [nN]*) then + set ANS_REQ_TED=$NO + else + goto ask_require_ted + endif + else + set ANS_REQ_TED=$DEF_REQ_TED + endif + + + +ask_require_misc: + echo -n "Compile other programs [$DEF_REQ_MISC]? " + set ANS_REQ_MISC = $< + if ("$ANS_REQ_MISC" == "?") then + echo "* Other programs include:" + echo " alfsplit" + echo " getABISampleName" + echo "" + goto ask_require_misc + else if ("$ANS_REQ_MISC" != "") then + if ("$ANS_REQ_MISC" =~ [yY]*) then + set ANS_REQ_MISC=$YES + else if ("$ANS_REQ_MISC" =~ [nN]*) then + set ANS_REQ_MISC=$NO + else + goto ask_require_misc + endif + else + set ANS_REQ_MISC=$DEF_REQ_MISC + endif + + + +time_taken_warning: + echo "" + echo "The installation procedure is now ready to start." + echo "" + echo "**** Warning:" + echo " The installation will take considerable time to complete. If you" + echo " are installing the whole Staden Package from scratch it could" + echo " take as long as an hour for all exectuables to be compiled and" + echo " installed." + echo "" + +ask_goahead: + echo -n "Proceed with the installation [YES]? " + set ANSWER=$< + if ("$ANSWER" == "?") then + echo "* Final confirmation to proceed with the installation. Answer" + echo " YES to proceed; otherwise, answer NO to abort the installation." + echo "" + goto ask_goahead + else if ("$ANSWER" != "") then + if ("$ANSWER" =~ [nN]*) then + goto chickens_exit + else if ("$ANSWER" !~ [yY]*) then + goto ask_goahead + endif + endif + +installation_proper: + +# make binaries directory if it doesn't exist + + if (! -d $DIR_BIN) then + $MKDIR $DIR_BIN + endif + + if ("$ANS_REQ_MISC" == "$YES" || "$ANS_REQ_X" == "$YES" || "$ANS_REQ_NONX" == "$YES" ) then + echo "" + echo "+ Compiling miscellaneous library" + + pushd $DIR_MISC > /dev/null + + cd $DIR_BINARIES + $MAKE all + + popd > /dev/null + + endif + + if ("$ANS_REQ_NONX" == "$YES") then + echo "" + echo "+ Installing non X programs" + + pushd $DIR_STADEN > /dev/null + cd $DIR_BINARIES + $MAKE nprogs lprogs + $INSTALL mep $DIR_BIN + $INSTALL nip $DIR_BIN + $INSTALL pip $DIR_BIN + $INSTALL sap $DIR_BIN + $INSTALL sapf $DIR_BIN + $INSTALL sip $DIR_BIN + $INSTALL splitp1 $DIR_BIN + $INSTALL splitp2 $DIR_BIN + $INSTALL splitp3 $DIR_BIN + $INSTALL sethelp $DIR_BIN + $INSTALL gip $DIR_BIN + $INSTALL nipl $DIR_BIN + $INSTALL pipl $DIR_BIN + $INSTALL sipl $DIR_BIN + $INSTALL dap $DIR_BIN + $INSTALL nipf $DIR_BIN + $INSTALL vep $DIR_BIN + $INSTALL rep $DIR_BIN + $INSTALL lip $DIR_BIN + #$INSTALL convert_project $DIR_BIN + popd > /dev/null + + pushd $DIR_OSP > /dev/null + cd $DIR_BINARIES + $MAKE + popd > /dev/null + + pushd $DIR_BAP > /dev/null + cd $DIR_BINARIES + $MAKE bap + $INSTALL bap $DIR_BIN + popd > /dev/null + + endif + + if ("$ANS_REQ_TED" == "$YES") then + echo "" + echo "+ Installing Trace editor" + + pushd $DIR_TED > /dev/null + cd $DIR_BINARIES + $MAKE ted + $INSTALL ted $DIR_BIN + popd > /dev/null + endif + + if ("$ANS_REQ_X" == "$YES") then + echo "" + echo "+ Installing X programs" + + pushd $DIR_STADEN > /dev/null + cd $DIR_BINARIES + $MAKE xprogs + $INSTALL xmep $DIR_BIN + $INSTALL xnip $DIR_BIN + $INSTALL xpip $DIR_BIN + $INSTALL xsap $DIR_BIN + $INSTALL xsip $DIR_BIN + $INSTALL xdap $DIR_BIN + popd > /dev/null + + pushd $DIR_OSP > /dev/null + cd $DIR_BINARIES + $MAKE + popd > /dev/null + + pushd $DIR_BAP > /dev/null + cd $DIR_BINARIES + $MAKE xbap + $INSTALL xbap $DIR_BIN + popd > /dev/null + + + endif + + if ("$ANS_REQ_MISC" == "$YES") then + echo "" + echo "+ Installing miscellaneous programs" + + pushd $DIR_ABI > /dev/null + cd $DIR_BINARIES + $MAKE all + $INSTALL getABISampleName $DIR_BIN + popd > /dev/null + + pushd $DIR_ALF > /dev/null + cd $DIR_BINARIES + $MAKE alfsplit + $INSTALL alfsplit $DIR_BIN + popd > /dev/null + + pushd $DIR_CONVERT > /dev/null + cd $DIR_BINARIES + $MAKE convert + $INSTALL convert $DIR_BIN + popd > /dev/null + + pushd $DIR_COP > /dev/null + cd $DIR_BINARIES + $MAKE all + $INSTALL cop $DIR_BIN + $INSTALL cop-bap $DIR_BIN + popd > /dev/null + + pushd $DIR_FROG > /dev/null + cd $DIR_BINARIES + $MAKE frog + $INSTALL frog $DIR_BIN + popd > /dev/null + + pushd $DIR_GETMCH > /dev/null + cd $DIR_BINARIES + $MAKE trace2seq + $INSTALL trace2seq $DIR_BIN + popd > /dev/null + + pushd $DIR_SCF > /dev/null + cd $DIR_BINARIES + $MAKE makeSCF + $INSTALL makeSCF $DIR_BIN + popd > /dev/null + + + + endif + + +installation_done: + echo "" + echo "+ Installation completed" + echo "" + + echo " Some further initialisation is required in order to use the" + echo " package. csh users should insert the following in their .login" + echo " files:" + echo " " + echo " setenv STADENROOT $ANS_STADEN_ROOT" + echo ' source $STADENROOT/staden.login' + echo " " + echo " Users of the Bourne shell, sh, should insert the following in" + echo " their .profile:" + echo " " + echo " STADENROOT=$ANS_STADEN_ROOT" + echo " export STADENROOT" + echo ' . $STADENROOT/staden.profile' + echo " " + echo " These initialisations will alter the shell's search path so that" + echo " it can find the programs in the STADEN Package" + echo " " + +normal_exit: + exit 0 + +chickens_exit: + echo "" + echo "+ Installation cancelled" + echo "" + + exit 0 + +end_failure: + unset noglob + echo "" + echo "Aborted STADEN Package installation on `date`" + echo "" + exit 1 + diff --git a/Staden_install-sun b/Staden_install-sun new file mode 100644 index 0000000..0d6711e --- /dev/null +++ b/Staden_install-sun @@ -0,0 +1,453 @@ +#! /bin/csh -f +# +# staden_install - version 2.4 +# +# This is a prototype installation program. +# +# 9 March 1992 +# Modified for installation on Sun, Alliant, etc +# No longer install 2rs +# +# 20 November 1992 +# Now includes convert, cop, frog, getMCH and scf +# +# 25 November 1992 +# SGI supported +# +# 19 May 1993 +# DEC Alpha, Solaris supported +# +# Written by sd@uk.ac.cam.mrc-lmb +# + +# prelim +set prog = $0 ; set prog = $prog:t + +# Machines supported: al sun dec sgi alpha solaris +#set MACHINE = `echo $prog | sed 's/.*-//'` +set MACHINE = sun + +# For local (MRC-LMB) setup only +#set LOCAL = `echo $prog | awk '/local/{print "YES";exit;}{print "NO";}'` +set LOCAL = NO + + +echo "" +echo -n "Staden Package installation procedure - " +switch (${MACHINE}) + case "al": + echo "Alliant FX/2800 Concentrix version" + set MAKE = "make -sk" + breaksw + case "sun": + echo "SunOS version" + set MAKE = "make -sk" + breaksw + case "dec": + echo "DEC Ultrix (mips) version" + set MAKE = "gmake -sk" + breaksw + case "sgi": + echo "Silicon Graphics Iris version" + set MAKE = "gmake -sk" + breaksw + case "alpha": + echo "DEC Alpha OSF/1 version" + set MAKE = "gmake -sk" + breaksw + case "solaris": + echo "Solaris version" + set MAKE = "make -sk" + breaksw + default: + echo "Panic. Unknown version" + exit 1 +endsw +echo "" +echo "* starting initialization...please wait." +echo "" + +# Binary fork of source directory +if ($LOCAL == "YES") then + set DIR_BINARIES = ${MACHINE}-binaries + set DIR_PROGS = ${MACHINE}-bin +else + set DIR_BINARIES = . + set DIR_PROGS = bin + set MAKE = "$MAKE -f makefile-${MACHINE}" +endif + +init: +# Set useful shell variables +set YES="YES"; +set NO="NO" + +# set/unset some .cshrc envs. +unset noclobber +set noglob + +# set interrupt trap +onintr end_failure + +# Make dir command +set MKDIR = "mkdir" + +# Copy command +set CP = "cp -p" + +# Install command +#set INSTALL = "install" +#set INSTALL = "mv" +set INSTALL = "cp" + +# Set up default responses +set DEF_STADEN_ROOT = `pwd` + +set DEF_REQ_NONX = "$YES" +set DEF_REQ_X = "$YES" +set DEF_REQ_TED = "$YES" +set DEF_REQ_MISC = "$YES" + +# directories +set DIR_SRC = $DEF_STADEN_ROOT/src +set DIR_BIN = $DEF_STADEN_ROOT/$DIR_PROGS +set DIR_MISC = $DIR_SRC/Misc +set DIR_STADEN = $DIR_SRC/staden +set DIR_TED = $DIR_SRC/ted +set DIR_ABI = $DIR_SRC/abi +set DIR_ALF = $DIR_SRC/alf +set DIR_BAP = $DIR_SRC/bap +set DIR_OSP = $DIR_SRC/bap/osp-bits +set DIR_CONVERT = $DIR_SRC/convert +set DIR_COP = $DIR_SRC/cop +set DIR_FROG = $DIR_SRC/frog +set DIR_GETMCH = $DIR_SRC/getMCH +set DIR_SCF = $DIR_SRC/scf + + +main: + + +preamble: + echo "" + echo "" + echo "* Please answer the following questions." + echo " Default answers to questions are given in square brackets." + echo " If you require help at any stage respond with a ? to the question." + echo "" + +ask_staden_root: + set ANS_STADEN_ROOT = $DEF_STADEN_ROOT + +ask_require_nonx_progs: + echo -n "Compile all the non-X programs in the Staden Package [$DEF_REQ_NONX]? " + set ANS_REQ_NONX = $< + if ("$ANS_REQ_NONX" == "?") then + echo "* If you do not have X windows on your system you will require" + echo " these. However, you will require Tektronics terminal emulation." + echo " If you do not require all of the non-X programs, you should abort" + echo " and manually make the ones you require." + echo "" + goto ask_require_nonx_progs + else if ("$ANS_REQ_NONX" != "") then + if ("$ANS_REQ_NONX" =~ [yY]*) then + set ANS_REQ_NONX=$YES + else if ("$ANS_REQ_NONX" =~ [nN]*) then + set ANS_REQ_NONX=$NO + else + goto ask_require_nonx_progs + endif + else + set ANS_REQ_NONX=$DEF_REQ_NONX + endif + +ask_require_x_progs: + echo -n "Compile all the X programs in the Staden Package [$DEF_REQ_X]? " + set ANS_REQ_X = $< + if ("$ANS_REQ_X" == "?") then + echo "* These are the programs that require X windows." + echo " If you do not require all of the X programs, you should abort" + echo " and manually make the ones you require." + + echo "" + goto ask_require_x_progs + else if ("$ANS_REQ_X" != "") then + if ("$ANS_REQ_X" =~ [yY]*) then + set ANS_REQ_X=$YES + else if ("$ANS_REQ_X" =~ [nN]*) then + set ANS_REQ_X=$NO + else + goto ask_require_nonx_progs + endif + else + set ANS_REQ_X=$DEF_REQ_X + endif + + +ask_require_ted: + echo -n "Compile the trace editing program ted [$DEF_REQ_TED]? " + set ANS_REQ_TED = $< + if ("$ANS_REQ_TED" == "?") then + echo "* This is the trace editor program. It allows you to look at" + echo " traces obtained from automated fluorescent sequencing machines." + echo "" + goto ask_require_ted + else if ("$ANS_REQ_TED" != "") then + if ("$ANS_REQ_TED" =~ [yY]*) then + set ANS_REQ_TED=$YES + else if ("$ANS_REQ_TED" =~ [nN]*) then + set ANS_REQ_TED=$NO + else + goto ask_require_ted + endif + else + set ANS_REQ_TED=$DEF_REQ_TED + endif + + + +ask_require_misc: + echo -n "Compile other programs [$DEF_REQ_MISC]? " + set ANS_REQ_MISC = $< + if ("$ANS_REQ_MISC" == "?") then + echo "* Other programs include:" + echo " alfsplit" + echo " getABISampleName" + echo "" + goto ask_require_misc + else if ("$ANS_REQ_MISC" != "") then + if ("$ANS_REQ_MISC" =~ [yY]*) then + set ANS_REQ_MISC=$YES + else if ("$ANS_REQ_MISC" =~ [nN]*) then + set ANS_REQ_MISC=$NO + else + goto ask_require_misc + endif + else + set ANS_REQ_MISC=$DEF_REQ_MISC + endif + + + +time_taken_warning: + echo "" + echo "The installation procedure is now ready to start." + echo "" + echo "**** Warning:" + echo " The installation will take considerable time to complete. If you" + echo " are installing the whole Staden Package from scratch it could" + echo " take as long as an hour for all exectuables to be compiled and" + echo " installed." + echo "" + +ask_goahead: + echo -n "Proceed with the installation [YES]? " + set ANSWER=$< + if ("$ANSWER" == "?") then + echo "* Final confirmation to proceed with the installation. Answer" + echo " YES to proceed; otherwise, answer NO to abort the installation." + echo "" + goto ask_goahead + else if ("$ANSWER" != "") then + if ("$ANSWER" =~ [nN]*) then + goto chickens_exit + else if ("$ANSWER" !~ [yY]*) then + goto ask_goahead + endif + endif + +installation_proper: + +# make binaries directory if it doesn't exist + + if (! -d $DIR_BIN) then + $MKDIR $DIR_BIN + endif + + if ("$ANS_REQ_MISC" == "$YES" || "$ANS_REQ_X" == "$YES" || "$ANS_REQ_NONX" == "$YES" ) then + echo "" + echo "+ Compiling miscellaneous library" + + pushd $DIR_MISC > /dev/null + + cd $DIR_BINARIES + $MAKE all + + popd > /dev/null + + endif + + if ("$ANS_REQ_NONX" == "$YES") then + echo "" + echo "+ Installing non X programs" + + pushd $DIR_STADEN > /dev/null + cd $DIR_BINARIES + $MAKE nprogs lprogs + $INSTALL mep $DIR_BIN + $INSTALL nip $DIR_BIN + $INSTALL pip $DIR_BIN + $INSTALL sap $DIR_BIN + $INSTALL sapf $DIR_BIN + $INSTALL sip $DIR_BIN + $INSTALL splitp1 $DIR_BIN + $INSTALL splitp2 $DIR_BIN + $INSTALL splitp3 $DIR_BIN + $INSTALL sethelp $DIR_BIN + $INSTALL gip $DIR_BIN + $INSTALL nipl $DIR_BIN + $INSTALL pipl $DIR_BIN + $INSTALL sipl $DIR_BIN + $INSTALL dap $DIR_BIN + $INSTALL nipf $DIR_BIN + $INSTALL vep $DIR_BIN + $INSTALL rep $DIR_BIN + $INSTALL lip $DIR_BIN + #$INSTALL convert_project $DIR_BIN + popd > /dev/null + + pushd $DIR_OSP > /dev/null + cd $DIR_BINARIES + $MAKE + popd > /dev/null + + pushd $DIR_BAP > /dev/null + cd $DIR_BINARIES + $MAKE bap + $INSTALL bap $DIR_BIN + popd > /dev/null + + endif + + if ("$ANS_REQ_TED" == "$YES") then + echo "" + echo "+ Installing Trace editor" + + pushd $DIR_TED > /dev/null + cd $DIR_BINARIES + $MAKE ted + $INSTALL ted $DIR_BIN + popd > /dev/null + endif + + if ("$ANS_REQ_X" == "$YES") then + echo "" + echo "+ Installing X programs" + + pushd $DIR_STADEN > /dev/null + cd $DIR_BINARIES + $MAKE xprogs + $INSTALL xmep $DIR_BIN + $INSTALL xnip $DIR_BIN + $INSTALL xpip $DIR_BIN + $INSTALL xsap $DIR_BIN + $INSTALL xsip $DIR_BIN + $INSTALL xdap $DIR_BIN + popd > /dev/null + + pushd $DIR_OSP > /dev/null + cd $DIR_BINARIES + $MAKE + popd > /dev/null + + pushd $DIR_BAP > /dev/null + cd $DIR_BINARIES + $MAKE xbap + $INSTALL xbap $DIR_BIN + popd > /dev/null + + + endif + + if ("$ANS_REQ_MISC" == "$YES") then + echo "" + echo "+ Installing miscellaneous programs" + + pushd $DIR_ABI > /dev/null + cd $DIR_BINARIES + $MAKE all + $INSTALL getABISampleName $DIR_BIN + popd > /dev/null + + pushd $DIR_ALF > /dev/null + cd $DIR_BINARIES + $MAKE alfsplit + $INSTALL alfsplit $DIR_BIN + popd > /dev/null + + pushd $DIR_CONVERT > /dev/null + cd $DIR_BINARIES + $MAKE convert + $INSTALL convert $DIR_BIN + popd > /dev/null + + pushd $DIR_COP > /dev/null + cd $DIR_BINARIES + $MAKE all + $INSTALL cop $DIR_BIN + $INSTALL cop-bap $DIR_BIN + popd > /dev/null + + pushd $DIR_FROG > /dev/null + cd $DIR_BINARIES + $MAKE frog + $INSTALL frog $DIR_BIN + popd > /dev/null + + pushd $DIR_GETMCH > /dev/null + cd $DIR_BINARIES + $MAKE trace2seq + $INSTALL trace2seq $DIR_BIN + popd > /dev/null + + pushd $DIR_SCF > /dev/null + cd $DIR_BINARIES + $MAKE makeSCF + $INSTALL makeSCF $DIR_BIN + popd > /dev/null + + + + endif + + +installation_done: + echo "" + echo "+ Installation completed" + echo "" + + echo " Some further initialisation is required in order to use the" + echo " package. csh users should insert the following in their .login" + echo " files:" + echo " " + echo " setenv STADENROOT $ANS_STADEN_ROOT" + echo ' source $STADENROOT/staden.login' + echo " " + echo " Users of the Bourne shell, sh, should insert the following in" + echo " their .profile:" + echo " " + echo " STADENROOT=$ANS_STADEN_ROOT" + echo " export STADENROOT" + echo ' . $STADENROOT/staden.profile' + echo " " + echo " These initialisations will alter the shell's search path so that" + echo " it can find the programs in the STADEN Package" + echo " " + +normal_exit: + exit 0 + +chickens_exit: + echo "" + echo "+ Installation cancelled" + echo "" + + exit 0 + +end_failure: + unset noglob + echo "" + echo "Aborted STADEN Package installation on `date`" + echo "" + exit 1 + diff --git a/Version-1993.0.7 b/Version-1993.0.7 new file mode 100644 index 0000000..1689cc5 --- /dev/null +++ b/Version-1993.0.7 @@ -0,0 +1,91 @@ +Wed Jul 7 + *Version-1993.0.7* + New xbap and ted. + Can use Ctrl as well as Meta to shift cutoffs in contig editor. + Code to read in ABI traces now robust to ABI problem files, where + called base order is not base position order. + +Thu Jul 1 + *Version-1993.0.6* + New xbap and bap, to fix bugs. + Break Contig was sometimes not recalculating consensus length correctly. + Contig Edit was trucating reading name lengths at 10 characters. + +Thu Jun 16 + *Version-1993.0.5* + New xbap and bap executables. RS changed assembly in bap so that + when entry is not permitted the program asks for the percentage + mismatch - this allows display of alignments for all levels of + mismatch. + +Mon Jun 14 14:54:43 BST 1993 + *Version-1993.0.4* + Bug in xdap. It was compiled with xbap's edUtils.h by mistake. + +Fri Jun 11 17:50:13 BST 1993 + *Version-1993.0.3* + Bugs in bap/xbap fixed. New executables included. + +Thu Jun 3 13:53:38 BST 1993 + *Version-1993.0.2* + Bugs in bap/xbap fixed. New executables included. + +Thu May 20 14:45:38 BST 1993 + *Version-1993.0.1* + Changes to makefiles and Staden_install + +Fri Mar 5 11:27:22 GMT 1993 + *Version-1993.0* + Now for DEC Alpha and Solaris + bap/xbap now includes double stranding and auto-creation of oligos + +Tue Jan 26 11:54:36 GMT 1993 + *Version-1992.3.1* + Bug fixes + 1. indexseqlibs/genbentryname1.c + 2. convert bugs + new programs + +Mon Nov 23 13:50:39 WET 1992 + *Version-1992.3* + Includes bap/xbap and utility programs + + +Wed Sep 30 11:18:09 BST 1992 + *Version-1992.2.1* + Source changes since last release + bug fixes to postscript output, sequence library programs + New sun and dec executables + + +Thu Aug 27 15:27:05 BST 1992 + + *Version-1992.2* + + +Mon Jul 27 13:01:37 WET 1992 + + *Version-1992.1.3* + Miscellaneous bug fixes and enhancements + New sun and dec executables + + +Tue Jun 16 16:07:41 BST 1992 + + *Version-1992.1.2* + Sun sparc executables now linked with cc and not gcc. + New makefile-sun files + New sources for hitNtrg.c and freetext4.c (indexseqlibs), and + tagU2.c (staden) + + +Wed May 27 17:12:36 BST 1992 + + *Version-1992.1.1* + Inclusion of vep (vector excision program), plus minor changes and bug fixes + + +Tue May 26 11:10:28 WET 1992 + + *Version-1992.1* + This version includes the port to DEC Ultrix (mips) + diff --git a/bin/alfsplit b/bin/alfsplit new file mode 100644 index 0000000000000000000000000000000000000000..97f5008a205c4f8f15902f1256a5759b26b4117d GIT binary patch literal 106496 zcmeFadwg71dGEbuW@~12i$@ph?s!|Wq{Mb?V~i6*inoIUAtZ4Kfrg%(c5KHXA#ReB z7@D?#H`kL-pj1(WD+GjztSBZmK^P;9OsZ*`7HD}-=jc^cp>lL4j!zKA7~vX6C>Ooo z-v^8_+}3q3g(9I+W3tAWG{5{m*Ui6uC*hh*B;|ER zQd>W_$_;mgQ%+adE2%Zc8*R>-h%xb}&5pR=VM1ZwI3X%}#+2lqC0FI1;rD&9CFOc1 z@%CK0u~g45JvV&&I_f{U^w>oeJUc3z%c%Q~PG}xXm^d_VQMxPPx_+OEbR z`a?gpy_EDjl%9&Dl21ocw;qe6Ztp8Hp-#uI2=HFr0WRtn)w;&)tIUSG6Q%~78+*v_ zhek)(RF6;>m{hX&&&Wd=*OUah_~_qdwc=f4u#kd+xaFeeP}B@7Vo;dv^uE)#q^KspLwqi3g_JQ(HS@!&4mzQ%rjuWEmQZ zrM7ye?(1p`wlHsN1ITo}ZxSO*fGAfNoPrF?sKb>(rpRp6G1u=q<jo&N zZYVF$MQDc$Xm`E}?b5|)V*@47D}H#DoA~J(kC*t!>24C8Q9iPa1bTlRG_D@09X^S^6Ay1lCAu1tj-N6moypB}BhBzd zd1u&l0$aMMK%W;{8o4&?)VpMzX-=*Q`2NF!y!AoedkgY5RvB|=M`f}D8+~g5Uh8jy zAE|T`;_D&!dU%6dlz$&6aYF+ax}gO&(ILCHHle!w7RnkrA>{o)!1vW7VfjMR5O-ri z-d6bwFng5-f06hgFHGK_6~GA32ZQvh$tR<7D--b(POhQOjSKSDlXu;Myp7~tt-M!} z&ydRgC*<>hXPjqMS90o*uG)uBLSqX$aIL&z!zVoxTPv6jWaXI)zN+^E-#Aako~ijK zm8*@|M?AC2GaV)F9JVQ$R;mm>uO1;ZDYlS5M}FBN`6c8>^ZD3YXrw)L_4?ttAFF

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zbZ)r0%AXkH^iA@-*e~(B;6I`ubVr}e>h6#j9e*1gkN?XXN0&fM? zat&BT^R+0j!ailLQ7(jW;ydX(Y^hoij81N~=fx@$!`!=&jx|-9()-lP;0y3sai0Jx zH(gnOBl4CwJi0lVM)frN+xO8o^aW}!ZI(6KJ;HqKPE?L*qqMeCEV-MTAucjsjaKQd z-_YKxeF^5U3-Hd~ef-brU!>O2XXIi2Oy{m3>oIPft6?9;+Ju_7hRjWMEHpoH3!0cH z6+1)*^K5Wh9VwZ~)6sK!KDreBDX|$j6zz%Zgctc~=*{Tgxq;yKuue9zI^qxDC-D{0 zCjNS#a*yeks5E7=vK=@Zd`!GAzYS%CO8ie`HQ6@$AciI<0xi+bSTxKPb&R4}=6guod`xIL9y-+yqvEzd$C`1{w@a zgEm3m!4%vS&W2~g74S90Lwbe8(49yv+5jDlE<;aa2G$#!hgIP^{sO)f|DLE%6cXD> zjO<1(CNERXsmatS`f>X8NB;)^gx>SRZBdu3EZN@c!8&atL;~oL-UG-h$j-_u${m&iq!bs9NGTkWH6kY^FLyx7D+T#Ex%q<% zfD~YOL1FIb5yg2qg+PyoVbl+oc3CBf^n(0EYHGMPf3zkV{y8<30y2v8Lr0I|oXnho zJ%$vgjmXW&DgruX<)`HgNY5J4X84HotddT}`JIaM+7u7Y$Qj->yQoXS$aXo|k1i}c rKLik-f4O6qw2oam_UHq2Y15_dBgY>ff0V!src.ps + +Now we can print this out: + + lpr src.ps + +Most of the above is only necessay if you are building something from +scratch, but it's best to go through it anyway until you fully +understand how LaTeX works. diff --git a/doc/gip-menu.PS b/doc/gip-menu.PS new file mode 100644 index 0000000..17d7616 --- /dev/null +++ b/doc/gip-menu.PS @@ -0,0 +1,131 @@ +%! +/cm {28.2 mul} def +/BOXSIZE 2 cm def + +/boxcen +{ +% move to centre of box +BOXSIZE mul 2 div BOXSIZE 2 div rmoveto +exch +% move back by correct amount to ensure letter is in centre of box +dup stringwidth +pop 2 div neg % halve & neg x offset +% y offset appears to be zero! - so use constant 'square' char (eg X) +(X) stringwidth pop 2 div neg +} def + +/letter +{ +dup BOXSIZE mul 0 rlineto +0 BOXSIZE rlineto +dup BOXSIZE mul neg 0 rlineto +0 BOXSIZE neg rlineto +closepath +gsave +dup boxcen rmoveto +show +stroke +grestore +BOXSIZE mul 0 rmoveto +} def + +/nextline {0 BOXSIZE neg rmoveto} def + +/line +{ +gsave +1 letter +1 letter +1 letter +1 letter +grestore +nextline +} def + +/Times-Roman findfont 50 scalefont setfont +newpath +5 setlinewidth +200 650 translate +0 0 moveto +%2 setlinecap + +gsave +(A) (G) (C) (T) line +(3) (4) (1) (2) line +(B) (H) (D) (V) line +(M) (N) (K) (L) line +(-) (X) (Y) (R) line +(8) (7) (6) (5) line +/Times-Roman findfont 25 scalefont setfont +gsave +(DELETE) 2 letter +(RESET) 2 letter +grestore +nextline +/Times-Roman findfont 35 scalefont setfont +gsave +(STOP) 4 letter +grestore +nextline +gsave +(START) 4 letter +grestore +nextline +gsave +(CONFIRM) 4 letter +grestore +nextline +% yukky from here on +gsave +0 BOXSIZE rmoveto +1 cm 0 rlineto stroke +grestore +(ORIGIN) dup 4 boxcen rmoveto show pop +(ORIGIN) stringwidth neg exch neg exch rmoveto +(X) stringwidth exch 2 div rmoveto +-5 0 rmoveto +2 setlinewidth +-45 21 rlineto +6 0 rlineto +-6 0 rmoveto +0 -6 rlineto +stroke +grestore +2 setlinewidth +0 BOXSIZE 1.4 mul rmoveto +6 6 rlineto +-6 -6 rmoveto +6 -6 rlineto +-6 6 rmoveto +80 0 rlineto +5 -6 rmoveto +/Times-Roman findfont 30 scalefont setfont +(8 cm) show +5 6 rmoveto +76 0 rlineto +-6 6 rlineto +6 -6 rmoveto +-6 -6 rlineto +stroke +0 0 moveto +BOXSIZE .4 mul neg BOXSIZE rmoveto +currentpoint translate +newpath +0 0 moveto +90 rotate +-6 6 rlineto +6 -6 rmoveto +-6 -6 rlineto +6 6 rmoveto +-244 0 rlineto +-84 0 rmoveto +0 -6 rmoveto +(20 cm) show +0 6 rmoveto +-84 0 rmoveto +-227 0 rlineto +6 6 rlineto +-6 -6 rmoveto +6 -6 rlineto +stroke +showpage diff --git a/doc/install.PS b/doc/install.PS new file mode 100644 index 0000000..0785781 --- /dev/null +++ b/doc/install.PS @@ -0,0 +1,2426 @@ +%! for use by dvi2ps Version 2.00 +% $Header: tex.ps,v 2.0 88/06/07 15:12:32 peterd Rel2 $ +% a start (Ha!) at a TeX mode for PostScript. +% The following defines procedures assumed and used by program "dvi2ps" +% and must be downloaded or sent as a header file for all TeX jobs. + +% By: Neal Holtz, Carleton University, Ottawa, Canada +% +% +% June, 1985 +% Last Modified: Aug 25/85 +% oystr 12-Feb-1986 +% Changed @dc macro to check for a badly formed bits in character +% definitions. Can get a <> bit map if a character is not actually +% in the font file. This is absolutely guaranteed to drive the +% printer nuts - it will appear that you can no longer define a +% new font, although the built-ins will still be there. +% mackay 4-Jan-1988 +% Changed size of character array to reflect gf usage (256 characters) + +% To convert this file into a downloaded file instead of a header +% file, uncomment all of the lines beginning with %-% + +%-%0000000 % Server loop exit password +%-%serverdict begin exitserver +%-% systemdict /statusdict known +%-% {statusdict begin 9 0 3 setsccinteractive /waittimeout 300 def end} +%-% if + +/TeXDict 200 dict def % define a working dictionary +TeXDict begin % start using it. + + % units are in "dots" (300/inch) +/Resolution 300 def +/Inch {Resolution mul} def % converts inches to internal units + +/Mtrx 6 array def + +%%%%%%%%%%%%%%%%%%%%% Page setup (user) options %%%%%%%%%%%%%%%%%%%%%%%% + +% dvi2ps will output coordinates in the TeX system ([0,0] 1" down and in +% from top left, with y +ive downward). The default PostScript system +% is [0,0] at bottom left, y +ive up. The Many Matrix Machinations in +% the following code are an attempt to reconcile that. The intent is to +% specify the scaling as 1 and have only translations in the matrix to +% properly position the text. Caution: the default device matrices are +% *not* the same in all PostScript devices; that should not matter in most +% of the code below (except for lanscape mode -- in that, rotations of +% -90 degrees resulted in the the rotation matrix [ e 1 ] +% [ 1 e ] +% where the "e"s were almost exactly but not quite unlike zeros. + +/@letter + { letter initmatrix + 72 Resolution div dup neg scale % set scaling to 1. + 310 -3005 translate % move origin to top (these are not exactly 1" + Mtrx currentmatrix pop % and -10" because margins aren't set exactly right) + } def + % note mode is like letter, except it uses less VM +/@note + { note initmatrix + 72 Resolution div dup neg scale % set scaling to 1. + 310 -3005 translate % move origin to top + Mtrx currentmatrix pop + } def + +/@landscape + { letter initmatrix + 72 Resolution div dup neg scale % set scaling to 1. +% -90 rotate % it would be nice to be able to do this + Mtrx currentmatrix 0 0.0 put % but instead we have to do things like this because what + Mtrx 1 -1.0 put % should be zero terms aren't (and text comes out wobbly) + Mtrx 2 1.0 put % Fie! This likely will not work on QMS printers + Mtrx 3 0.0 put % (nor on others where the device matrix is not like + Mtrx setmatrix % like it is on the LaserWriter). + 300 310 translate % move origin to top + Mtrx currentmatrix pop + } def + +/@legal + { legal initmatrix + 72 Resolution div dup neg scale % set scaling to 1. + 295 -3880 translate % move origin to top + Mtrx currentmatrix pop + } def + +/@manualfeed + { statusdict /manualfeed true put + statusdict /manualfeedtimeout 300 put % 5 minutes + } def + % n @copies - set number of copies +/@copies + { /#copies exch def + } def + +%%%%%%%%%%%%%%%%%%%% Procedure Defintions %%%%%%%%%%%%%%%%%%%%%%%%%% + +/@newfont % id @newfont - -- initialize a new font dictionary + { /newname exch def + pop + newname 7 dict def % allocate new font dictionary + newname load begin + /FontType 3 def + /FontMatrix [1 0 0 -1 0 0] def + /FontBBox [0 0 1 1] def +% mackay 4-Jan-1987 changed size of array from 128 to 256 for gf fonts + /BitMaps 256 array def + /BuildChar {CharBuilder} def + /Encoding 256 array def + 0 1 255 {Encoding exch /.undef put} for + end + newname newname load definefont pop + } def + + +% the following is the only character builder we need. it looks up the +% char data in the BitMaps array, and paints the character if possible. +% char data -- a bitmap descriptor -- is an array of length 6, of +% which the various slots are: + +/ch-image {ch-data 0 get} def % the hex string image +/ch-width {ch-data 1 get} def % the number of pixels across +/ch-height {ch-data 2 get} def % the number of pixels tall +/ch-xoff {ch-data 3 get} def % number of pixels below origin +/ch-yoff {ch-data 4 get} def % number of pixels to left of origin +/ch-tfmw {ch-data 5 get} def % spacing to next character + +/CharBuilder % fontdict ch Charbuilder - -- image one character + { /ch-code exch def % save the char code + /font-dict exch def % and the font dict. + /ch-data font-dict /BitMaps get ch-code get def % get the bitmap descriptor for char + ch-data null eq not + { ch-tfmw 0 ch-xoff neg ch-yoff neg ch-width ch-xoff sub ch-height ch-yoff sub + setcachedevice + ch-width ch-height true [1 0 0 1 ch-xoff ch-yoff] + {ch-image} imagemask + } + if + } def + + +/@sf % fontdict @sf - -- make that the current font + { setfont() pop + } def + + % in the following, the font-cacheing mechanism requires that + % a name unique in the particular font be generated + +/@dc % char-data ch @dc - -- define a new character bitmap in current font + { /ch-code exch def +% ++oystr 12-Feb-86++ + dup 0 get + length 2 lt + { pop [ <00> 1 1 0 0 8.00 ] } % replace <> with null + if +% --oystr 12-Feb-86-- + /ch-data exch def + currentfont /BitMaps get ch-code ch-data put + currentfont /Encoding get ch-code + dup ( ) cvs cvn % generate a unique name simply from the character code + put + } def + +/@bop0 % n @bop0 - -- begin the char def section of a new page + { + } def + +/@bop1 % n @bop1 - -- begin a brand new page + { pop + erasepage initgraphics + Mtrx setmatrix + /SaveImage save def() pop + } def + +%-- tjh sept. 87: if this page has a mac drawing on it, we have to +%-- use showpage in the md dictionary. +/@eop % - @eop - -- end a page + { + userdict /md known { + userdict /md get type /dicttype eq { + md /MacDrwgs known { + md begin showpage end + }{ + showpage + } ifelse + }{ + showpage + } ifelse + }{ + showpage + } ifelse + SaveImage restore() pop + } def + +/@start % - @start - -- start everything + { @letter % (there is not much to do) + } def + +/@end % - @end - -- done the whole shebang + { end + } def + +/p % x y p - -- move to position + { moveto + } def + +/r % x r - -- move right + { 0 rmoveto + } def + +/s % string s - -- show the string + { show + } def + +/c % ch c - -- show the character (code given) + { c-string exch 0 exch put + c-string show + } def + +/c-string ( ) def + +/ru % dx dy ru - -- set a rule (rectangle) + { /dy exch neg def % because dy is height up from bottom + /dx exch def + /x currentpoint /y exch def def % remember current point + newpath x y moveto + dx 0 rlineto + 0 dy rlineto + dx neg 0 rlineto + closepath fill + x y moveto + } def + +%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% +%% the \special command junk +%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% +% The structure of the PostScript produced by dvi2ps for \special is: +% @beginspecial +% - any number of @hsize, @hoffset, @hscale, etc., commands +% @setspecial +% - the users file of PostScript commands +% @endspecial + +% The @beginspecial command recognizes whether the Macintosh Laserprep +% has been loaded or not, and redfines some Mac commands if so. +% The @setspecial handles the users shifting, scaling, clipping commands + +%-- tjh sept. 87: made changes to allow postscript and macdrawing to +%-- to be inserted with version 65 of the md dictionary. Many bugs +%-- were fixed: +%-- vo changed to vof, name conflict with md +%-- vs changed to vsz, name conflict with md +%-- substantially changed @setspecial and @MacSetUp +%-- Also, made changes to allow users to specify offsets +%-- and clip rectangles in inches. + +% The following are user settable options from the \special command. + +/@SpecialDefaults + { /hs 8.5 72 mul def + /vsz 11 72 mul def + /ho 0 def + /vof 0 def + /hsc 1 def + /vsc 1 def + /CLIP false def + } def + +% d @hsize - specify a horizontal clipping dimension +% these 2 are executed before the MacDraw initializations +/@hsize {72 mul /hs exch def /CLIP true def} def +/@vsize {72 mul /vsz exch def /CLIP true def} def + +% d @hoffset - specify a shift for the drwgs +/@hoffset {72 mul /ho exch def} def +/@voffset {72 mul /vof exch def} def + +% s @hscale - set scale factor +/@hscale {/hsc exch def} def +/@vscale {/vsc exch def} def + +/@setclipper + { hsc vsc scale + CLIP + { newpath 0 0 moveto hs 0 rlineto 0 vsz rlineto hs neg 0 rlineto closepath clip } + if + } def + +% this will be invoked as the result of a \special command (for the +% inclusion of PostScript graphics). The basic idea is to change all +% scaling and graphics back to defaults, but to shift the origin +% to the current position on the page. Due to TeXnical difficulties, +% we only set the y-origin. The x-origin is set at the left edge of +% the page. + +/@beginspecial + { gsave /SpecialSave save def + % the following magic incantation establishes the current point as + % the users origin, and reverts back to default scalings, rotations + currentpoint transform initgraphics itransform translate + @SpecialDefaults % setup default offsets, scales, sizes + @MacSetUp % fix up Mac stuff + } def + + +%-- tjh: assume this is raw postscript, but save some state in case its not. +/@setspecial + { + /specmtrx matrix currentmatrix def + ho vof translate @setclipper + } def + + +/@endspecial + { SpecialSave restore + grestore + } def + + +% - @MacSetUp - turn-off/fix-up all the MacDraw stuff that might hurt us + % we depend on 'psu' being the first procedure executed + % by a Mac document. We redefine 'psu' to adjust page + % translations, and to do all other the fixups required. + % This stuff will not harm other included PS files +/@MacSetUp + { userdict /md known % if md is defined + { userdict /md get type /dicttype eq % and if it is a dictionary + { + md begin % then redefine some stuff + /psu % redfine psu to set origins, etc. + /psu load + % this procedure contains almost all the fixup code + { +% /letter {} def % it is bad manners to execute the real +% /note {} def % versions of these (clears page image, etc.) +% /legal {} def + /MacDrwgs true def + specmtrx setmatrix % restore pre-@setspecial state. + initclip % ditto + % change smalls to prevent page clearing. + /smalls [ lnop lnop lnop lnop lnop lnop lnop lnop lnop ] def + 0 0 0 0 ppr astore pop % prevents origin translation. + % redifine cp, do the showpage later, see @eop + /cp { + pop + pop + pm restore + } def % no printing of pages + } + concatprocs + def + /od + % redefine od to translate and scale. + % redfine load to set clipping region. + /od load + { + ho vof translate + hsc vsc scale + CLIP { + /nc + /nc load + { newpath 0 0 moveto hs 0 rlineto 0 vsz rlineto + hs neg 0 rlineto closepath clip } + concatprocs + def + } if + } + concatprocs + def + end } + if } + if + } def + +% p1 p2 concatprocs p - concatenate procedures +/concatprocs + { /p2 exch cvlit def + /p1 exch cvlit def + /p p1 length p2 length add array def + p 0 p1 putinterval + p p1 length p2 putinterval + p cvx + } def + +end % revert to previous dictionary +TeXDict begin @start +%%Title: install.dvi +%%Creator: dvi2ps +%%EndProlog +1 @bop0 +[ 300 ] /cmr17.300 @newfont +cmr17.300 @sf +[ 24 49 -3 0 23.499] 73 @dc +[ 40 31 -2 0 36.644] 110 @dc +[<80FE00C301C0CC0060F00030F00038E00018E0001CC0001CC0001C80001C80003C80003C0000F80001F8003FF003FFE00FFF + C01FFF003FF0007E0000F80000F00010E00010E00010E00010E000306000303000701800F00E033001FC10> 24 31 -2 0 25.776] 115 @dc 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780001E000F80001E000F80001E000F80001E000F80001E000F80001E000F80001E000F80001E000F80001E000F80001E000 + 780001E0007C0001E0003C0001E0003C0001E0001E0001E0000E0003E0000F0003E000078005E00001C019E00000F061E000 + 001F81E000000001E000000001E000000001E000000001E000000001E000000001E000000001E000000001E000000001E000 + 000001E000000001E000000001E000000001E000000001E000000001E000000003E00000003FE00000003FE000000001E000> 40 50 -3 0 36.644] 100 @dc +[ 40 49 -4 0 45.061] 80 @dc +[<003F800000E0600003801800070004000F0002001E0002001E0001003C0001007C0000007C00000078000000F8000000F800 + 0000F8000000F8000000F8000000F8000000F8000000F8000000F8000000780000007C0008007C003E003C003E001C003E00 + 1E001E000F000400070004000380180000E06000003F8000> 32 31 -3 0 29.149] 99 @dc +[ 40 50 -2 0 34.770] 107 @dc +[ 300 ] /cmr12.300 @newfont +cmr12.300 @sf +[<81FC00C60700C80180F000C0E000C0C00060C000608000708000708000708000700000700000F00000F00001E00007E0003F + 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s +15 r (the) s +15 r (pac) s +0 r 107 c +-3 r (age.) s +925 2776 p 52 c +@eop +@end diff --git a/doc/install.tex b/doc/install.tex new file mode 100644 index 0000000..37515cc --- /dev/null +++ b/doc/install.tex @@ -0,0 +1,172 @@ +\documentstyle[a4,11pt]{article} + +\title{Installing the Staden Package} +\author{Simon Dear} +\date{21 May 1993} + + + +\begin{document} +\maketitle + + + +\section{Introduction} + +On the accompanying tape you will find executables for +one of SunOS 4.x, Sun +Solaris 2.x, DEC Ultrix, DEC OSF/1 and Silicon Graphics SGI operating systems. +Also there are sources for all the programs in the Staden package. +Programs in the package are: +\begin{description} + +\item[mep and xmep] Motif exploration program. +\item[nip and xnip] Nucleotide interpretation program. +\item[nipl] Nucleotide interpretation program (library). +Searches nucleotide libraries for patterns of motifs. +\item[pip and xpip] Protein interpretation program. +\item[pipl] Protein interpretation program (library). +Searches protein libraries for patterns of motifs. +\item[sip and xsip] Similarity investigation program. +\item[sipl] Similarity investigation program (library). +Compares a probe protein or nucleic acid sequence against +a library of sequences. +\item[sap and xsap] The original sequence assembly program. +\item[bap and xbap] Our latest, most advanced sequence assembly program. +\item[dap and xdap] An obsolete assembly program, superceded by {\em bap}. +\item[lip] Library interface program. +\item[rep] Repeat examination program. +\item[ted] X windows utility for displaying and editing +fluorescent sequencing machine traces. +\item[splitp1, splitp2 and splitp3] Refer to help/SPLITP.MEM. +\item[sethelp] Builds online help files. +\item[gip] Gel input program. +\item[convert] Converts between {\em xdap\/} and {\em xbap\/} databases. +\item[cop and cop-bap] Checks completed {\em xdap\/} and {\em xbap\/} +databases for editing errors. +\item[trace2seq] Extracts sequence from trace files. +\item[getABISampleName] Extracts sample names from ABI trace files. +\item[makeSCF] Converts existing trace files to the compact +SCF format. +\item[alfsplit] Splits the Pharmacia A.L.F. gel +file into multiple files, one for each sample. +\item[frog] Relabels lanes in ABI trace files. +\item[+ numerous scripts (including {\em squirrel (v1.4)\/})] + +\end{description} + + +\section{Requirements} + +You will need a tape drive to read the software off the distribution +tape (QIC-150, TK50, or Exabyte). You will also need a large amount of +disk storage to accommodate the whole package. For release +version-1993.0, requirements were +31Mb (SunOS 4.x), +36Mb (Sun Solaris 2.x) +30Mb (DEC Ultrix) +37Mb (DEC OSF/1) +and +27Mb (Silicon Graphics SGI.) + + +To compile the Staden package you will require: +\begin{itemize} +\item An ANSI C compiler. +\item A FORTRAN-77 compiler. +\item X11 (Release 4 or 5). +\item GNU make (except with SunOS and Solaris 2.x.) +\end{itemize} + +\section{Installation} + +To install the package, +\begin{enumerate} +\item Create a directory for where you would like the software to be +placed. You may have to be superuser to do this. + \begin{verbatim} mkdir /home/Staden\end{verbatim} +\item Change to this directory. + \begin{verbatim} cd /home/Staden\end{verbatim} +\item Place the tape into the tape unit. +\item Extract the software off the distribution tape (NOTE: the device name may be +different on your machine): + \begin{verbatim} tar xvf /dev/rst0\end{verbatim} +\item C shell users should set the environment variable {\bf STADENROOT} +to be the directory where the package is installed and source the file +{\em staden.login} found there. This is best done by adding lines to their +{\em .login} file: +\begin{verbatim} + setenv STADENROOT /home/Staden + source $STADENROOT/staden.login +\end{verbatim} +Users of the Bourne shell, sh, should similarly add lines their {\em .profile} file: +\begin{verbatim} + STADENROOT=/home/Staden + export STADENROOT + . $STADENROOT/staden.profile +\end{verbatim} + +The startup routines set environment variables and modify the shell's +search path so that it can find the programs in the Staden Package. +When users next log on to the system, they will be able to use the +programs. + +\end{enumerate} + + +\section {Installation on Unsupported Platforms} + +Install the software as you would for a supported machine. You will +need to remake all executables. The script {\em Staden\_install} can +be used to help recompile the package. A large number of +assumptions have been made, and you may need to change the makefiles +to suit your system. + +The sources have been organised into subdirectories of the directory +{\bf src}. In {\bf Misc} are routines common to many programs. They +should be made first. In {\bf staden} are all the programs of the +Staden suite ({\em mep}, {\em nip}, {\em pip}, {\em sap}, {\em sip}, +{\em dap}, {\em gip}, {\em vep}, {\em lip} and {\em rep}) with the +exception of {\em bap}. Code for our latest sequence assembly program +{\em bap} is in directories {\bf bap} and {\bf bap/osp-bits}. Make +the objects in {\bf staden} first, then the ones in {\bf +bap/osp-bits}, and finally the ones in {\bf bap}. In {\bf ted} is the +trace editing program. + + +\section {Other Software Provided} + +Other software and scripts can be found in the {\bf alf\/}, {\bf +abi\/}, {\bf cop\/}, {\bf getMCH\/}, {\bf scf\/}, {\bf frog\/} and {\bf +scripts} +directories. +Each directory contains documentation describing the programs +contained. + +Since release version-1993.0 we have distributed the {\em squirrel (v1.4)} +package. Please read the disclaimer that accompanies this software. +Additional sources and scripts can be found in {\bf expGetSeq}, {\bf vepe}, +{\bf newted} and {\bf squirrel-1.4} directories. + +Many scripts (including {\em squirrel}) and filters were developed at the MRC-LMB for +{\bf INTERNAL USE ONLY}. +We are aware that people elsewhere will want to develop +similar software. +We include them in the Staden Package merely as {\bf EXAMPLES} of +what has been achieved elsewhere. +{\bf THESE SCRIPTS WILL NOT WORK ON YOUR SYSTEM WITHOUT MODIFICATION.} + +\section {When All Else Fails...} +If you have any problems please contact the authors, +\mbox{Rodger Staden} +\mbox{(\em rs@mrc-lmba.cam.ac.uk\/)}, +\mbox{Simon Dear} +\mbox{(\em sd@mrc-lmba.cam.ac.uk\/)} +and +\mbox{James Bonfield} +\mbox{(\em jkb@mrc-lmba.cam.ac.uk\/)}, +by email or by writing to us at: +MRC Laboratory of Molecular Biology, Hills Road, Cambridge, \mbox{CB2 2QH}, U.K. +We also welcome general comments on the package. + +\end{document} diff --git a/doc/manual.rtf b/doc/manual.rtf new file mode 100644 index 0000000..cbc023c --- /dev/null +++ b/doc/manual.rtf @@ -0,0 +1,5154 @@ +{\rtf1\mac\deff2 {\fonttbl{\f0\fswiss Chicago;}{\f2\froman New York;}{\f3\fswiss Geneva;}{\f4\fmodern Monaco;}{\f5\fscript Venice;}{\f6\fdecor London;}{\f7\fdecor Athens;}{\f8\fdecor San Francisco;}{\f11\fnil Cairo;}{\f12\fnil Los Angeles;} +{\f13\fnil Zapf Dingbats;}{\f14\fnil Bookman;}{\f15\fnil N Helvetica Narrow;}{\f16\fnil Palatino;}{\f18\fnil Zapf Chancery;}{\f20\froman Times;}{\f21\fswiss Helvetica;}{\f22\fmodern Courier;}{\f23\ftech Symbol;}{\f24\fnil Mobile;}{\f33\fnil Avant Garde;} +{\f34\fnil New Century Schlbk;}}{\colortbl\red0\green0\blue0;\red0\green0\blue255;\red0\green255\blue255;\red0\green255\blue0;\red255\green0\blue255;\red255\green0\blue0;\red255\green255\blue0;\red255\green255\blue255;}{\stylesheet{\s243\qc\sa60\sl280 +\f20 \sbasedon222\snext0 footer;}{\s244\sl220\tqc\tx4320\tqr\tx8640 \f4\fs16 \sbasedon0\snext0 header;}{\sl220 \f4\fs16 \sbasedon222\snext0 Normal,Screen Font;}{\s2\qc\sa200\sl480 \b\f20\fs36 \sbasedon222\snext2 Chapter Heading;}{\s3\sb200\sa120\sl360 +\b\f20\fs32 \sbasedon222\snext0 Main Subheading;}{\s4\qj\sa120\sl280 \f20 \sbasedon222\snext4 Body text;}{\s5\sb400\sa60\sl320\tx560 \b\f20\fs28 \sbasedon222\snext5 Subheading;}{\s6\sb240\sa60\sl280\tx560\tx860 \b\f20 \sbasedon5\snext6 SubSub heading;}{ +\s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 \sbasedon4\snext7 Indent Body;}{\s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 \sbasedon222\snext8 Figure legends;}{\s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 \sbasedon6\snext9 SubSubSub heading;}} +\paperw11880\paperh16820\margl1440\margr1440\widowctrl\ftnbj\ftnrestart \sectd \linemod0\linex0\cols1\endnhere \pard\plain \s2\qc\sa600\sl480 \b\f20\fs36 {\i\fs48 Contents\par +}\pard\plain \s7\qj\fi-560\li560\sa120\sl400\tx560\tqr\tldot\tx8980 \f20 1\tab Preface\tab 1\par +2\tab Introduction\tab 3\par +3\tab Sequence input, editing and sequence library use\tab 17\par +4\tab Managing sequencing projects\tab 26\par +5\tab Analysing sequences to find genes\tab 51\par +6\tab Searching for motifs in nucleic acid sequences\tab 60\par +7\tab Using patterns to analyse nucleic acid sequences\tab 69\par +8\tab Searching for restriction sites\tab 77\par +9\tab Statistical and structural analysis of nucleotide sequences\tab 83\par +10\tab Translating and listing nucleic acid sequences\tab 93\par +11\tab Statistical and structural analysis of protein sequences\tab 99\par +12\tab Searching for motifs in protein sequences\tab 104\par +13\tab Using patterns to analyse protein sequences\tab 112\par +\pard \s7\qj\fi-560\li560\sa120\sl400\tx560\tqr\tldot\tx8980 14\tab Comparing sequences\tab 123\par +\pard\plain \s2\qc\sa200\sl480\tqr\tldot\tx8980 \b\f20\fs36 \sect \sectd \pgnrestart\linemod0\linex0\cols1\endnhere {\footer \pard\plain \s243\qc\sa60\sl280 \f20 \chpgn \par +}\pard\plain \s2\qc\sa600\sl480 \b\f20\fs36 1. Preface (November, 1992)\par +\pard\plain \s4\qj\sa120\sl280 \f20 This second edition of the manual contains only minor revisions. The changes are mostly to do with managing sequencing pro +jects which is the subject on which we are currently concentrating our efforts. We have replaced our previous Developing Assembly Program DAP with another developing assembly program BAP that can assemble Bigger projects. Although this new program can hand +le 8000 readings as opposed to the miserly 1000 of the previous version, it actualy uses its space more efficiently over the course of a project. It contains a mechanism for preventing simultaneous use (and hence corruption) of databases. In addition it is + approximately four times faster during assembly and five times faster when looking for "internal joins". It now contains a routine for selecting primers and templates during the "walking" stage of a project . The "find internal joins" function now calls u +p the contig joining editor with the two contigs aligned in the window and the editor has also been speeded up. Numerous other changes have also been made but we still regard BAP as temporary, and are actively working on its replacement which we believe wi +ll overcome the limitations that BAPs aged structure has imposed on it. We have also included routines for converting ABI 373A and Pharmacia A.L.F. data to our new trace file format, for automatically marking poor quality regions of readings from these mac +hines and for converting DAP databases to BAP databases.\par +\pard \s4\qj\sa120\sl280 Other changes include providing a postscript option for saving graphics output, and facilities for using the author and freetext indexes of the sequence libraries. The sequence library indexes are v +ery useful and allow rapid searching. The freetext index is derived from ALL the text in the annotations - not just the keywords. We have also added a new repeat examining routine in NIP and a new repeat listing option in SIP.\par +\pard \s4\qj\sa120\sl280 \par +\pard\plain \s2\qc\sa600\sl480 \b\f20\fs36 1. 1 Preface to first edition \par +\pard\plain \s4\qj\sa120\sl280 \f20 +It could be said that this manual is long overdue, for, apart from the extensive online help available from within the programs, it is the first printed guide to using a package that has been around for longer than I care remember. On the other hand, to + misquote a cliche much used by reviewers, it could be said that this manual fills a much needed gap, in that I believe the best way to learn about computer programs is to use them. Those who are prepared to experiment and play with programs will discover +far more than any manual of reasonable size can hope to convey. However the manual serves to give users an overview of what is available and a starting point for their exploration of the programs.\par +\pard \s4\qj\sa120\sl280 One of my objectives was to be able to distribute the manua +l on floppy disk so that each site using the programs could print as many copies as they need. We had to balance the quality of the graphics and the sophistication of the layout, against the ease of producing updates and the availability of software, and d +ecided to to use the WORD4 program running on the Apple Macintosh. The graphics figures reproduced in the manual are far below the quality seen on the terminal screen, and in some cases should be viewed as merely schematic.\par +\pard \s4\qj\sa120\sl280 Most of the chapters are self-contained but users are strongly advised to read sections 3 to 7 in chapter 1, as to do so will save a lot of time.\par +\pard \s4\qj\sa120\sl280 In future editions we will add chapters on other programs in the package and expand the Notes sections to give more information about the theory and algorithms used. We welcome comments and suggestions for improvements.\par +\pard \s4\qj\sa120\sl280 I thank Brian Pashley for transforming my original documents into, what I hope will be, a useful manual.\par +\pard\plain \s3\sb200\sa120\sl360 \b\f20\fs32 Rodger Staden, March 1992.\par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 2. Introduction\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +2.\tab Materials\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1720 2.1\tab Versions\par +2.2\tab Terminals\par +2.3\tab Digitizers\par +2.4\tab Sequencing machines\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab User interfaces\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 3.1\tab The xterm and VAX interface\par +3.2 \tab The X interface\par +3.3\tab Use of the bell\par +3.4\tab Printing and saving results in files\par +3.5\tab Use of feature tables\par +3.6\tab Use of graphics\par +3.7\tab The active region\par +3.8\tab Files of file names\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Character sets\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1720 4.1\tab Character sets for finished sequences\par +4.2\tab Symbols used in gel readings\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Sequence formats\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1720 5.1\tab Personal sequence files\par +5.2\tab Sequence libraries\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Conventions used in text\par +7.\tab Notes\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 +In this chapter we give an overview of the chapters on the "Staden Package" of programs. Here we describe the equipment required and outline the scope of the package and the user interfaces. In the next chapter we cover character sets, sequence formats and + sequence library access.\par +\pard \s4\qj\sa120\sl280 The main programs in the package are as follows\:\par +\pard\plain \s7\qj\sa120\sl280\tx1120 \f20 GIP\tab Gel input program\par +\pard \s7\qj\sa120\sl280\tx1120\tx1580 SAP\tab Sequence assembly program\par +\pard \s7\qj\sa120\sl280\tx1120 BAP\tab Sequence assembly program\par +NIP\tab Nucleotide interpretation program\par +PIP\tab Protein interpretation program\par +SIP\tab Similarity investigation program\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx1120 MEP\tab Motif exploration program\par +NIPL\tab Nucleotide interpretation program (library)\par +PIPL\tab Protein interpretation program (library)\par +SIPL\tab Similarity investigation program (library)\par +XBAP\tab Sequence assembly program\par +XNIP\tab Nucleotide interpretation program\par +XPIP\tab Protein interpretation program\par +XSIP\tab Similarity investigation program\par +XMEP\tab Motif exploration program\par +\pard\plain \s4\qj\sa120\sl280 \f20 GIP uses a digitiser for entry of DNA sequences from autoradiographs. SAP, BAP and XBAP handle everything relating to assembling and edi +ting gel readings. NIP provides functions for analysing and interpretting individual nucleotide sequences. PIP provides functions for analysing and interpretting individual protein sequences. MEP analyses families of nucleotide sequences to help discover n +ew motifs. NIPL performs pattern searches on nucleotide sequence libraries. PIPL performs pattern searches on protein sequence libraries. SIP provides functions for comparing and aligning pairs of protein or nucleotide sequences. SIPL searches nucleotide a +nd protein sequence libraries for entries similar to probe sequences. The programs whose names begin with a letter X are X11 (see below) versions of the programs. For example XNIP is an X11 version of NIP.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 2.\tab Materials\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.1\tab Versions.\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The programs run on Apple Macintosh computers, on VAX computers using the VMS operating system, and on SUN workstations (which use the UNIX operating system.) The SUN version should run, with only minor changes, on other machines running UNIX and currently + we are aware of versi +ons running on DEC ULTRIX, Silicon Graphics, Alliant FX2800 and Convex machines. Currently the Macintosh version is "frozen" in its April 1990 state, the VAX version is "frozen" in its April 1991 state and all development is being done on the SUN version. +\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.1.1\tab VAX version.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The VAX version will run on any VAX using the VMS operating system. A FORTRAN compiler is required.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.1.2\tab UNIX version.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The UNIX version is being used here on SPARCstations and DECstation 5000/240s with at least 8 megabytes of memory, 20 +0 megabyte internal disk drives and 700 megabyte external disks. Colour monitors such as the GX are preferable for running the programs which display traces from fluorescent sequencing machines, but monochrome displays are adequate for all other programs. +We also use tape desktop backup packs for archiving, and a cdrom drive for handling the sequence libraries.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.1.3\tab Other UNIX versions.\par +\pard\plain \s4\qj\sa120\sl280 \f20 Users of UNIX machines other than SUN SPARCstations, DECstation 5000/240 and SGI Indigo R3000 will require a FORTRAN comp +iler and ANSI C. When operated directly on the workstation screen all UNIX versions require X11 release 4.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.1.4\tab The Macintosh version\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The Macintosh version of the package requires a machine with at least 1 megabyte of memory and a 20 megabyte hard disk. It only operates on monochrome screens or colour screens set to black/white mode. The package contains only programs SAP, GIP, NIP, PIP + and SIP. All further information about this version of the package is contained in the notes.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.2\tab Terminals.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The program +s can also be operated via a serial port using Tektronix terminals, PC's running MS-Kermit, or Apple Macintoshs running Versaterm Pro. The UNIX versions can also be run from X teminals or microcomputers running X emulators.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.3\tab Digitizers.\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The gel reading input program uses a sonic digitizer called a GRAPHBAR GP7 made by Science Accessories Corp., 200 Watson Blvd., Stratford, CT 06497, USA. When ordering specify that the device should be set to use metric units.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.4\tab Sequencing machines.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The programs can handle data produced by the Applied Biosystems Inc. 373A and Pharmacia A.L.F fluorescent sequencing machines.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 3.\tab User Interfaces\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The programs have two user interfaces. The first runs under the terminal emulator xterm and the second runs directly under X. On the VAX, at present only the xterm interface is available, but on UNIX systems either interface can be used. The xterm version +of the package will operate on the workstation screen, X terminals, Tektronix terminals, PC's or Macintoshes (see above). When run + on the workstation screen the programs have separate text and graphics windows, each of which can be moved, resized and iconized, and the text windiow can be scrolled in both directions. The versions that run directly under X can only be used on the works +tation screen, X terminals or using an X emulator. They produce separate text and graphics windows, an independent, constantly available help window and a separate dialogue window. All input is controlled by mouse selection and dialogue boxes.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 3.1\tab The xterm and VAX interface\par +\pard\plain \s4\qj\sa120\sl280 \f20 The user interface is common to all programs. It consists of a set of menus and a uniform way of presenting choices and obtaining input from the user. This section describes\: + the menu system; how options are selected and other choices made; how values are +supplied to the program; how help is obtained, and how to escape from any part of a program. In addition it gives information about saving results in files and the use of graphics for presenting results.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.1.1.\tab Menus and option selection\par +\pard\plain \s4\qj\sa120\sl280 \f20 +Each program has several menus and numerous options. Each menu or option has a unique number that is used to identify it. Menu numbers are distinguished from option numbers by being preceded by the letter m (or M, all programs make no distinction between u +pper and lower case letters). With the exception of some parts of program SAP, the menus are not hierachical, rather the options they each contain are simply lists of related functions and their identifying numbers. Therefore options can be selected inde +pendently of the menu that is currently being shown on the screen, and the menus are simply memory aides. All options and menus are selected by typing their option number when the programs present the prompt \par +\pard \s4\qc\sb120\sa180\sl280 "? Menu or option number =" \par +\pard \s4\qj\sa120\sl280 +To select a menu type its number preceded by the letter M. To select an option type its number. If users type only "return" they will get menu m0 which is simply a list of menus. If users select an option they will return to the current menu after the func +tion is completed. Where possible, equivalent or identical options have been given the same numbers in all programs, and so users quickly learn the numbers for the functions they employ most often.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.1.2\tab Execution and dialogue\par +\pard\plain \s4\qj\sa120\sl280 \f20 +All inputs requested by the program (apart from file names) have default values. In addition most of the analytical functions have a default path through which they will pass, so when users select an option, in many cases the program will immediately perfo +rm the operation selected without further dialo +gue. However if users precede an option number by the letter d (e.g. D17), they will force the program to offer dialogue about the selected option before the function operates, hence allowing them to change the value of any of its parameters. In addition, +alternative suboptions will be made available.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.1.3\tab Help\par +\pard\plain \s4\qj\sa120\sl280 \f20 Help about each option can be obtained by preceding the option number by the symbol ? when users are presented with the prompt "? Menu or option number", (e.g. ?17 gives help on the option 17), but +there are two further ways of obtaining help. Whenever the program asks a question users can respond by typing the symbol ? and they will receive information about the current option. In addition, option number 1 in all the programs will give help on all o +f a programs functions. \par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.1.4.\tab Quitting \par +\pard\plain \s4\qj\sa120\sl280 \f20 To exit from any point in a program users type ! for quit. If a menu is on the screen this will stop the program, otherwise they will be returned to the last menu. \par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.1.5.\tab Making selections\par +\pard\plain \s4\qj\sa120\sl280 \f20 Questions and choices are dealt with in three ways. Where there are choices that are not obvious opposites, or there are more than two choices, "radio buttons" and "check boxes" are used.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\pagebb\tx1140 \b\f20 3.1.5.1.\tab Choosing between opposites.\par +\pard\plain \s4\qj\sa120\sl280 \f20 Obvious opposites such as "clear screen" and "keep picture" are presented with only the default shown. For example in this case the default is generally "keep picture" so the program will display\: \par +\pard\plain \li1720\sa200\sl220 \f4\fs16 "Keep picture (y/n) (y) =" \par +\pard\plain \s4\qj\sa120\sl280 \f20 and the picture will be retained if the user types Y or y or only return. If the user types N or n the picture will be cleared. Anything other than these or ? or ! will cause the question to be asked again.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.1.5.2. \tab Choosing one from many.\par +\pard\plain \s4\qj\sa120\sl280 \f20 +Radio buttons are used when only one of a number of choices can be made at any one time. The choices are presented arranged one above the other, each choice with a number for its selection, and the default choice marked with an X. For example when the user + is reading a new sequence file the following choices of format are offered.\par +\pard\plain \li1720\sb300\sl220\tx2460\tx3400 \f4\fs16 Select sequence file format\par +\pard \li1720\sl220\tx2460\tx3400 \tab 1\tab Staden\par +\tab 2\tab EMBL\par +X\tab 3\tab GenBank\par +\tab 4\tab PIR\par +\tab 5\tab GCG\par + 6 FASTA\par +\pard \li1720\sa300\sl220\tx2460\tx3400 ? Selection (1-5) (3) =\par +\pard\plain \s4\qj\sb60\sa120\sl280 \f20 Any single option can be selected by typing the option number, and the default option, (here shown as 3), is also obtained by typing only "return". Again help can be obtained by typing ? and quit by typing !. +\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.1.5.3.\tab Choosing at least one from many.\par +\pard\plain \s4\qj\sa120\sl280 \f20 Check boxes are used when any number of a set of choices can be made (i.e. the choices are not exclusive). Choices are made by typing choice numbers. Each choice c +an be considered as a switch whose setting is reversed when it is selected. Choices that are currently switched on are marked with an X. The user quits from making selections by typing only "return". For example in the routine that plots base composition u +sers can elect to plot the frequencies of any combination of bases, e.g. only A, or A+T, or A+T+G etc. The following check box is offered to the user\: \par +\pard\plain \li1720\sb300\sl220\tx2420\tx3400 \f4\fs16 X\tab 1\tab T\par +\pard \li1720\sl220\tx2420\tx3400 \tab 2\tab C\par +X\tab 3\tab A\par +\tab 4\tab G\par +\pard \li1720\sa300\sl220\tx2420\tx3400 ? Selection (1-4) ( ) =\par +\pard\plain \s4\qj\sb60\sa120\sl280 \f20 As shown this will plot the A+T composition. To switch off T select 1, to switch on C select 2, etc, to quit, having set the bases required type only "return". \par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 \page 3.1.6.\tab Input of numerical values \par +\pard\plain \s4\qj\sa120\sl280 \f20 All input of integer or decimal numbers is presented in a standard way with the allowed range shown in brackets and the default value also in brackets. For example\: \par +\pard\plain \li1700\sb160\sa300\sl220 \f4\fs16 ? Window (5-31) (11) = \par +\pard\plain \s4\qj\sa120\sl280 \f20 In this example users could type any number between 5 and 31, or "return" only, or ! or ? (see above). Any other input will cause the program to ask the question again. Typing only "r +eturn" gives the default value (here 11). \par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.1.7.\tab Input of character strings\par +\pard\plain \s4\qj\sa120\sl280 \f20 Character strings are requested using informative prompts of the form\:\par +\pard\plain \li1720\sb160\sa300\sl220 \f4\fs16 ? Search string =\par +\pard\plain \s4\qj\sa120\sl280 \f20 Or where possible the prompt will be preceded by a default value as in\:\par +\pard\plain \li1720\sb160\sl220 \f4\fs16 Default search string = atatatata\par +\pard \li1720\sa300\sl220 ? Search string =\par +\pard\plain \s4\qj\sa120\sl280 \f20 Question mark (?) or ! will get help or quit. Where appropriate, for example when a whole list of strings have been defined one after the other, typing return only will be a signal to the program that input is complete. +\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 3.2.\tab The X interface\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This interface deals with all the types of interactions described above but options are selected using pulldown menus and all inputs are via appropriately styled dialogue boxes and buttons. Default values are accepted by clicking on an "OK" button, or typi +ng return on the keyboard. Values are changed by overtyping the defaults. Quit is available from each dialogue via a "CANCEL" button. Help is constantly available via a "HELP" button in the main dialogue window. Details such as requestin +g dialogue when an option is selected are dealt with using a button labelled "execute with dialogue" which toggles to "execute".\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 3.3.\tab Use of the bell \par +\pard\plain \s4\qj\sa120\sl280 \f20 The programs use the bell to indicate that a task is completed. When the bell sounds, the programs will wait until return is typed. Users can quit from these points by typing ! but no help is available.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 3.4.\tab Printing and saving results in files \par +\pard\plain \s4\qj\sa120\sl280 \f20 A few of the functions in the programs automatically write their textual results to disk files, but for most functi +ons users can choose whether results appear on the terminal screen or go to a file. For these functions the normal, or default, place for results to appear is on the screen, and users need to decide before the function is selected if they want to redirect +the results to a file. In all programs the option "Redirect output" gives control over whether results appear on the screen or go to a file. When a program is started results will be sent to the screen. If the option "Redirect output" is selected users wil +l be given the choice of redirecting either text or graphics to a file or of creating a postscript file for the graphics. The program will then ask users to supply a file name. If users elect to redirect output, from that point on ,all results will be sent + to the file until the option is selected again, in which case the "redirection file" will be closed, and results will again appear on the screen. If these files contain textual results they can be looked at from within the programs by using option "List +a text file". Once the program is left users can employ an appropriate system command to print the files. There is no function within the programs to direct files to a printer. If users elect to create a postscript file for the graphics the graphics will a +lso appear on the screen. If they redirect graphics the graphics commands (in Tektronix codes) will only go to the file and will not appear on the screen\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 3.5.\tab Use of feature tables\par +\pard\plain \s4\qj\sa120\sl280 \f20 One particular use of redirection should be noted. The programs can use EMB +L/GenBank feature tables as input for directing translation of DNA to protein, etc, but the tables must be stored in separate text files, and cannot be read directly from the sequence libraries. The only routines that can read the sequence libraries are th +ose available under "Read a sequence". So to create a text file containing the feature table for a particular library entry users must redirect text output to disk, and then use the "Read a sequence" to display the appropriate feature table. The feature ta +ble will be written to the file, and then the file can be used for controlling translation etc. Note however that the redirection mechanism is a general function and it therefore does not add the required header and tail to saved files. To make the files u +seable as feature tables they need, as a minimum, a line at the top with the word FEATURES starting in column 1, and two empty lines at the end of the file!\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 3.6.\tab Use of graphics \par +\pard\plain \s4\qj\sa120\sl280 \f20 The analytical programs including NIP, PIP and SIP present the results of many of their analyses graphically.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.6.1.\tab The drawing board and plot positions\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The position at which the results for any function appear on the screen is defined relative to a notional users "drawing board" of dimension 10,000 by 10,000. This drawing board fills the screen and results are drawn in windows defined using symbols x0,y0 +and xlength,ylength, where x0,y0 is the position of the bottom left hand corner of the window, and xlength is the width of the window and ylength the height of the window. The win +dow positions for each option are read from a file when a program is started. If required individual users can have their own set of plot positions, and also the positions can be redefined from within the programs using the option "Reposition plots". +\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.6.2.\tab The plot interval\par +\pard\plain \s4\qj\sa120\sl280 \f20 +For those analyses that draw continuous lines to represent results (for example a plot of base composition) the user is asked to supply the "Plot interval". All the analyses produce a value for every point along the sequence but often i +t is unnecessary to actually plot the values for all the points. The plot interval is simply the distance between the points shown on the screen. If the user selects a plot interval of 1, every point will be plotted; a plot interval of 3 will show every th +ird point. \par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.6.3.\tab The window length\par +\pard\plain \s4\qj\sa120\sl280 \f20 The word "window" is used in a further way by the programs. Most of the functions that analyse the content of a sequence (the simplest such routine plots the base composition) perform their calculations over a segment o +f the sequence of a certain length, display the result, then move on by 1 position, and recalculate. The fixed size of segment over which a calculation is performed is called a "window" and the segment size is the "window length". Many analytical functions + request "? Window length =", or more frequently "? Odd window length =". An odd number is used so that when a result is displayed for a particular window position it is derived from an equal number of points either side of the windows' midpoint.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.6.4.\tab Use of the cross hair\par +\pard\plain \s4\qj\sa120\sl280 \f20 +All programs that produce graphical output provide a function for using a cross hair to examine the plots. After the cross hair function is selected the cross will appear in the graphics window and can be steered around using the mouse or directional keys. + Special keyboard characters hit while the function is in operation produce the following results. For all programs the letter s (for sequence) will show the local sequence around the cross hair position. For the sequence comparison pro +grams that show a dot matrix the two sequences will be displayed above one another. For the sequencing project management programs all the aligned sequences in the contig will be displayed. For the sequence comparison programs the letter m (for matrix) wil +l show a matrix in which all identical characters for a window around the cross hair are marked. The punctuation symbol , will show the local position in sequence units, but leave the cross hair on the screen, whereas the space bar and any other non-specia +l character will show the local position and exit the cross hair function. Further special characters are defined in the chapter on managing sequencing projects.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.6.5\tab Drawing scales on plots\par +\pard\plain \s4\qj\sa120\sl280 \f20 All the programs have a function "Draw a ruler" which will allow users to add scales to the axes of graphical plots. The scale can be positioned anywhere on the plot.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 3.6.6\tab Saving graphics\par +\pard\plain \s4\qj\sa120\sl280 \f20 The best way of saving the graphics is to use the "Redirect output" function to open a postscript file which will then contain a co +py of all plots that appear on the screen. This of course requires the file to be opened before the plots are drawn. Many terminals are not capable of dumping their screen contents to a file for subsequent printing. One convenient way of obtaining hard cop +y of graphical results is to use a micro computer as a terminal. On the Macintosh we use the terminal emulator versa termPro. This allows graphics to be saved as Macintosh files that can be annotated and printed using Macdraw and other painting programs. A +lternatively graphics can be redirected to a file and printed using a laser printer with tektronix capability (see "Printing and saving results in files"). \par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 3.7.\tab The active region\par +\pard\plain \s4\qj\sa120\sl280 \f20 +All the analytical programs use an "active region" for most of their functions. This is simply the current section of the sequence over which the analysis will be applied. When a sequence is first read in the active region will be set to its whole length, +but the user can restrict the scope of analytical functions by use of an opt +ion called "Define active region". However some functions such as "List the sequence" are always given access to the whole sequence and will allow the user to define a limited range after they have been selected.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 3.8.\tab Files of file names\par +\pard\plain \s4\qj\sa120\sl280 \f20 +A useful device that is employed by many of the programs is that of "files of file names". If a program needs to perform the same operation in turn on each of 20 files, the user should not have to type in 20 file names. Instead the user types in the name o +f a single file which contains the names of the other 20 files. This single file is a file of file names. They are used, for example, to process batches of gel readings, or to compare a sequence against a library of motifs.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 4.\tab Character Sets\par +\pard\plain \s4\qj\sa120\sl280 \f20 There are two types of character sets employed by the programs\: those for finished sequences and those used during sequencing projects.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 4.1\tab Character sets for finished sequences\par +\pard\plain \s4\qj\sa120\sl280 \f20 The analytical programs will operate with uppercase or lowercase sequence characters. For nucleic acids T and +U are equivalent. For proteins the standard 1 letter codes are used. The analytical programs also use IUB symbols for redundancy in back translations and for sequence searches. The symbols are shown in table 2.1 \par +\pard \s4\qj\li2260\ri2220\sb300\sa120\sl280\box\brsp100\brdrth \tx3420\tx4800 A,C,G,T\par +\pard \s4\qj\li2260\ri2220\sa120\sl280\box\brsp100\brdrth \tx3420\tx4800 R\tab (A,G)\tab 'puRine'\par +Y\tab (T,C)\tab 'pYrimidine'\par +W\tab (A,T)\tab 'Weak'\par +S\tab (C,G)\tab 'Strong'\par +M\tab (A,C)\tab 'aMino'\par +K\tab (G,T)\tab 'Keto'\par +H\tab (A,T,C)\tab 'not G'\par +B\tab (G,C,T)\tab 'not A'\par +V\tab (G,A,C)\tab 'not T'\par +D\tab (G,A,T)\tab 'not C'\par +\pard \s4\qj\li2260\ri2220\sa120\sl280\keepn\box\brsp100\brdrth \tx3420\tx4800 N\tab (G,A,C,T)\tab 'aNy'\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Table 1.1\tab The NC-IUB characters used by the analytical programs\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 4.2\tab Symbols used in gel readings\par +\pard\plain \s4\qj\sa120\sl280 \f20 Th +e information stored about a sequence reading has to show the original sequence, recording any doubts about its interpretation, and also, where possible, allow the changes made during editing to be indicated. Lowercase characters are used by the sequence p +roject management programs for recording readings, and uppercase symbols are used when changes are made during editing. Alternatively the reverse convention can be used. Any other characters in a sequence are treated as dash (-) characters. The symbols are + shown in table 2.2.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 5.\tab Sequence Formats\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The data formats for the programs that deal with sequencing projects are described in the chapter on managing sequencing projects. All analytical programs can read sequences stored in several formats. We distinguish between two sources of input namely\: + "sequence libraries" and "personal files".\par +\pard \s4\qj\sa120\sl280 \par +\pard \s4\qj\li1120\ri1200\sa120\sl280\box\brsp100\brdrth \tqc\tx2800 {\b Symbol \tab Meaning}\par +\pard \s4\qj\li1120\ri1200\sl280\box\brsp100\brdrth \tx1400\tqc\tx2800\tqc\tx4240\tqc\tx5640\tx6820 \tab c\tab Definitely\tab c\par +\tab t\tab "\tab t\par +\tab a\tab "\tab a\par +\tab g\tab "\tab g\par +\tab 1\tab Probably\tab c\par +\tab 2\tab "\tab t\par +\tab 3\tab "\tab a\par +\tab 4\tab "\tab g\par +\tab d\tab "\tab c\tab Possibly\tab cc\par +\tab v\tab "\tab t\tab "\tab tt\par +\tab b\tab "\tab a\tab "\tab aa\par +\tab h\tab "\tab g\tab "\tab gg\par +\tab k\tab "\tab c\tab "\tab c-\par +\tab l\tab "\tab t\tab "\tab t-\par +\tab m\tab "\tab a\tab "\tab a-\par +\tab n\tab "\tab g\tab "\tab g-\par +\tab r\tab a or g\par +\tab y\tab c or t\par +\tab 5\tab a or c\par +\tab 6\tab g or t\par +\tab 7\tab a or t\par +\tab 8\tab g or c\par +\tab -\tab a or g or c or t\par +\pard \s4\qj\li1120\ri1200\sl280\box\brsp100\brdrth \tx1400\tqc\tx3780\tqc\tx4240\tqc\tx5640\tx6820 \tab A\tab a set by auto edit or corrected by user\par +\tab C\tab c set by auto edit or corrected by user\par +\tab G\tab g set by auto edit or corrected by user\par +\tab T\tab t set by auto edit or corrected by user\par +\pard \s4\qj\li1120\ri1200\sl280\box\brsp100\brdrth \tx1400\tqc\tx4020\tqc\tx5640\tx6820 \tab *\tab padding character placed by auto assembler\par +\pard \s4\qj\li1120\ri1200\sl280\keepn\box\brsp100\brdrth \tx1400\tqc\tx2800\tqc\tx4240\tqc\tx5640\tx6820 else = -\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa300\sl240\tx1140 \f21\fs20 Table 2.2\tab The symbols used to record gel readings\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 5.1\tab Personal sequence files\par +\pard\plain \s4\qj\sa120\sl280 \f20 The programs can read sequences from files in PIR, EMBL, GenBank, GCG, FASTA and Staden formats. Staden format + means text files with records of up to 80 characters; all spaces are removed; lines with ";" in the first position are treated as comments and will be displayed when the file is read but not included in the sequence; if the first line of data contains a 2 +0 character header of the form <---abcdefghij-----> it too will not be included in the processed sequence. This last facility allows the programs to read consensus sequences created by the sequence project management programs. Files in PIR format can conta +in any number of entries (which the user selects by entry name), but all other formats are expected to contain only one sequence. If they contain more only the first will be read.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 5.2\tab Sequence libraries\par +\pard\plain \s4\qj\sa120\sl280 \f20 +Users may not appreciate the fact that because the sequence libraries are so large, programs need to use indexes to provide rapid retrieval of individual entries. An index is a list of entry names and pairs of offsets. For each entry name the offsets defin +e the position at which its sequence and annotation s +tart in the large file. The index, which is in any case relatively small, is arranged so that it can be searched quickly - for example the EMBL cdrom index is sorted alphabetically. When the user supplies an entry name the program rapidly finds it in the i +ndex file and then uses the associated offsets to locate the entry in the larger sequence files.\par +\pard \s4\qj\sa120\sl280 The sequence libraries are stored in different ways on the VAX and the SUN. On the VAX we adopted the widely used PIR format and indexing method and on the SUN we use the EMBL cdrom format and indexes.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 5.2.1\tab Sequence libraries on the VAX\par +\pard\plain \s4\qj\sa120\sl280 \f20 +On the VAX all libraries are stored in PIR format, and except for the facility to select entries by accession number, the same functions are provided as those on the SUN. Note that this means that most libraries need reformatting after they have been read + from the distribution media. Because, for each entry, the sequence and its annotation are stored separately, the reformatting process consumes significant computer resources. T +hese reformatting programs are available from PIR and we give no further information here. The programs that search whole libraries of sequences also expect the libraries to be in PIR format.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 5.2.2.\tab Sequence libraries for the UNIX version\par +\pard\plain \s4\qj\sa120\sl280 \f20 +For the UNIX version of the programst we use the EMBL cdrom as the primary source of sequence data and have chosen their indexing method for all libraries. These indexes leave the sequence libraries in their distribution format and simply provide offsets t +o the original fi +les. The cdrom provides the EMBL nucleic acid sequence library and the SWISSPROT protein sequence library. Currently it also includes indexes for entry names, accession numbers, authors and freetext and has an additional "title" file which, for each entry, + consists of entry name, entry length and an 80 character description of the entry. These indexes allow rapid retrieval of entries by name or accession number, and the author and freetext indexes can be searched very rapidly. The files can be left on the +cdrom or transfered to a hard disk. The programs that search whole libraries of sequences expect the libraries to be in cdrom format or PIR format.\par +\pard \s4\qj\sa120\sl280 +We have written our own programs for producing EMBL cdrom type indexes for other sequence libraries. These allow us to use the PIR protein libraries in CODATA format and between release updates of the EMBL nucleotide library. Others may wish to use them to + produce indexes for libraries such as GenBank. In addition to our own programs the scripts that produce the indexes also use the UNIX sort program. We give no further details here but the programs are described in Staden and Dear, 1992.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 5.2.2.1\tab Library description files.\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The following information is only relevent to those installing the sequence libraries on a SUN. To make the sequence library handling as flexible as possible we use several level of files. As stated above, at present we only deal with the EMBL and SWISSPRO +T libraries as distributed on cdrom and the PIR protein library in CODATA format. By including a "library type" flag in the library description file we also leave open the possibility of using alternative formats. \par +\pard \s4\qj\sa120\sl280 We describe the libraries at 3 levels\: + 1) a list of libraries and their types, which points to 2) the files which name the libraries individual files and their file types, then, finally 3) the librairies individual files. The files used are described below.\par +\pard\plain \s7\qj\fi-1120\li1120\sa120\sl280\pagebb\tx1120 \f20 Level 1)\tab The top level file is a list of available libraries which contains\: the library type, the name of the file containing th +e names of each libraries individual files, and the prompt to appear on the users screen. \par +\pard\plain \s4\qj\sa120\sl280 \f20 Example\: \par +\pard \s4\qj\li1100\sa120\sl280 File name\: SEQUENCELIBRARIES\par +File contents\:\par +\pard\plain \li1120\sl220 \f4\fs16 A\tab EMBLLIBDESCRP EMBL nucleotide library ! in cdrom format\par +A\tab SWISSLIBDESCRP SWISSPROT protein library! in cdrom format\par +\pard \li1120\sa300\sl220 B\tab PIRLIBDESCRP PIR protein library! in CODATA format\par +\pard\plain \s4\qj\sa180\sl280 \f20 The first two libraries are of type A. The logical names are EMBLLIBDESCRP and SWISSLIBDESCRP, and the prompts are "EMBL nucleotide library" and "SWISSPROT protein library". The third library is o +f type B with logical name PIRLIBDESCRP. Space is used as a delimiter and anything to the right of a ! is a comment.\par +\pard\plain \s7\qj\fi-1100\li1100\sa120\sl280\tx1120 \f20 Level 2)\tab The file containing the names of the libraries individual files contains flags to define the file types and the path or logical names of the files. Current file types are\: \par +\pard\plain \fi100\li980\sl220 \f4\fs16 A\tab Division_lookup\par +B\tab Entryname_index\par +C\tab Accession_target\par +D\tab Accession_hits\par +E\tab Brief_directory.\par +F\tab Freetext_target\par +G\tab Freetext_hits\par +H\tab Author_target\par +I\tab Author_hits\par +\pard\plain \s4\qj\sa120 \f20 Example\par +\pard \s4\qj\li1120\sa120 File name\: EMBLLIBDESCRP\par +File contents\:\par +\pard\plain \fi100\li980\sl220 \f4\fs16 A\tab STADTABL/EMBLdiv.lkp\par +B\tab /cdrom/indices/embl/entrynam.idx\par +C\tab /cdrom/indices/embl/acnum.trg\par +D\tab /cdrom/indices/embl/acnum.hit\par +E\tab /cdrom/indices/embl/brief.idx\par +F\tab /cdrom/indices/embl/freetext.trg\par +G\tab /cdrom/indices/embl/freetext.hit\par +H\tab /cdrom/indices/embl/author.trg\par +I\tab /cdrom/indices/embl/author.hit\par +\pard \li1120\sa300\sl220 \par +\pard\plain \s7\qj\fi-1120\li1120\sa120\sl280\tx1120 \f20 Level 3)\tab +The individual library files. The contents of all files below Division_lookup are exactly as they appear on the cdrom. The Division_lookup file is rewritten so the directory structure and file names can be chosen locally. Its format is I6,1x,A. \par +\pard\plain \s4\qj\sb300\sa180\sl280 \f20 The files which define all the programs and standard data files used by the package\: + staden.login and staden.profile, define the file SEQUENCELIBRARIES which contains the list of available libraries. As should be clear from the description above the three +levels need to be created (actually modified from the contents of the distribution tape) and all names can be changed locally, or set to be the same as those on the cdrom.\par +\pard\plain \s7\qj\fi-1120\li1120\sa120\sl280\tx1120 \f20 \par +\pard\plain \s4\qj\sa120\sl280 \f20 Example of Division_lookup file \par +\pard \s4\qj\li1120\sa120\sl280 File name\: STADTABL/EMBLdiv.lkp\par +Contents\:\par +\pard\plain \li1120\sl220 \f4\fs16 1\tab /cdrom/embl/fun.dat\par +2\tab /cdrom/embl/inv.dat\par +3\tab /cdrom/embl/mam.dat\par +4\tab /cdrom/embl/org.dat\par +5\tab /cdrom/embl/phg.dat\par +6\tab /cdrom/embl/pln.dat\par +7\tab /cdrom/embl/pri.dat\par +8\tab /cdrom/embl/pro.dat\par +9\tab /cdrom/embl/rod.dat\par +10\tab /cdrom/embl/syn.dat\par +11\tab /cdrom/embl/una.dat\par +12\tab /cdrom/embl/vrl.dat\par +13\tab /cdrom/embl/vrt.dat\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 6.\tab Conventions Used In Text\par +\pard\plain \s4\qj\sa120\sl280 \f20 Obviously the programs can perform many more operations than there is space to describe but, in the selection of uses shown, we have tried to give some feel for the programs' sco +pe. For this reason, and the need to conform as closely as possible to the format of the book, we have chosen specific paths through the programs, rather than attempt to describe all routes. For some sections, such as that on the facilities available for e +diting contigs, this has not been possible and we have instead described how the major commands are used. It should also be noted that the user interactions described in the methods sections are those that would be required if the options were selected in +the "Execute with dialogue" mode. In practice many of the options would normally be used without any dialogue being required.\par +\pard \s4\qj\sa120\sl280 +In the section on the user interface we outlined the different modes of obtaining input from users. Throughout the specific chapters we have adopted the following conventions to indicate which mode of input is being employed. When a program requests numeri +cal or string input we have used the term "Define", as in Define "Minimum search score". When a program requests that a choice is +made between several options, as in the case of radio buttons or check boxes, we have used the term "Select". When a program offers a choice between two options in the form of a yes or no answer, as in "Hide translation", we use the terms "Accept" or "Reje +ct". When the digitizer program uses the stylus for input we have used the term "Hit".\par +\pard \s4\qj\sa120\sl280 Because it is difficult to produce figures including pull down menus and dialogue boxes, almost all examples containing user input are taken from the xterm interface. Ho +wever the actual wording of the prompts is the same for both interfaces.\par +\pard \s4\qj\sa120\sl280 +The programs contain routines for drawing scales on plots and for simple annotation, but in general such embellishment is not done automatically by the programs. This is because the programs are designed so that many plots can be superimposed, and it is be +tter for the user to explicitly decide to add scales and annotation. More elaborate annotation can be added by saving the graphics output to files which can be handled by, say Macinto +sh, painting and drawing programs. None of the examples of graphical results shown in the following chapters have added scales\: all are exactly as drawn by the programs.\par +\pard\plain \s5\sa60\sl320\tx560 \b\f20\fs28 \par +\par +\par +\par +7.\tab NOTES\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 7.1\par +\pard\plain \s4\qj\sa120\sl280 \f20 +Although all the programs in the Macintosh version of the package work, the conversion to this machine was never finished. The package does not provide access to the sequence libraries, handling only simple text files containing sequences, or those generat +ed by the assembly program SAP. The user interface, although using pu +ll down menus and dialogue boxes for all interactions, is not as "Mac like" as many would expect. However many people find this version very useful, and for others, the digitizer program alone makes the package worth having. Data input from a digitizer is +a task suited to a machine like the Macintosh, and the data files can be transferred to a larger machine for assembly and other analysis. With the exception of sequence library access, all the options available in the 1990 VAX version are contained in the +package (See Staden, 1990). We give no further details specific to the Macintosh version.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 8.\tab References\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Staden, R. 1990. An improved sequence handling package that runs on the Apple Macintosh. Comput. {\i Applic. Biosc}. {\b 4}, 387-393.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Staden, R. and Dear, S. 1992. Indexing the sequence libraries\: Software providing a common indexing system for all the standard sequence libraries. {\i DNA Sequence} {\b 3}, 99-105.\par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 3. Sequence Input, Editing and Sequence Library Use\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 1.1\tab Introduction to sequence input\par +1.2 \tab Introduction to keyboard input\par +1.3\tab Introduction to input from digitizer\par +1.4\tab Introduction to editing single sequences\par +1.5\tab Introduction to using the sequence libraries\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Methods\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.1\tab Sequence input from keyboard\par +2.2\tab Sequence input from digitizer\par +2.3\tab Sequence input from the Pharmacia A.L.F.\par +2.4\tab Sequence input from the ABI 373A.\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.5\tab Editing a nucleic acid sequence using restriction sites and a translation and base numbering as landmarks.\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.6\tab Searching the freetext and author indexes of a sequence library\par +2.7\tab Using accession numbers to retrieve data from a sequence library\par +2.8\tab Displaying the annotations for an entry in a sequence library\par +2.9\tab Reading a sequence from sequence library\par +2.10\tab Worked example of sequence library access\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Notes\par +4.\tab References\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 In this chapter we describe sequence input and editing and the use of sequence libraries.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 1.1\tab Introduction to sequence input and editing\par +\pard\plain \s4\qj\sa120\sl280 \f20 The package contains facilities for input of sequence data from the keyboard, sonic digitizer +s, and ABI 373A and Pharmacia A.L.F fluorescent sequencing machines. Editing of single sequences can be performed using system editors such as EDT on the VAX and EMACS on the SUN. Editing of sequence alignments is discussed in the chapter on managing sequ +encing projects.\par +\pard\plain \s6\sa60\sl280\pagebb\tx560\tx860 \b\f20 1.2\tab Introduction to keyboard input\par +\pard\plain \s4\qj\sa120\sl280 \f20 The program SAP contains an option to enter sequence at the keyboard. It also creates a file of file names and will list the sequences. Users may choose any 4 keys to represent the characters A, C, G and +T. For example 4 adjacent keys in the same order as the lanes on a gel could be used. The program translates these symbols to A, C, G and T, and any other characters are left unchanged. No line of input should be longer than 80 characters. Terminate input +with the symbol @.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 1.3\tab Introduction to input from digitizer\par +\pard\plain \s4\qj\sa120\sl280 \f20 Digitisers provide a convenient way of entering sequences from films into a computer. The digitiser, which is connected directly to the computer, operates on a light box, and is controlled by a pr +ogram named GIP (1). The film to be read is taped firmly to the surface of the light box, and the user defines the lane order and the centres of the four lanes to be read. These positions are defined at the point where reading will commence and the program + adjusts their values as the film is read. The user reads the sequence and transfers it to the computer by hitting the centres of the bands progressing up the film. Any number of sets of lanes and films can be read in a single run of the program. Each sequ +ence is stored in a separate file and a file of file names is also written. The program also uses a menu, which is a series of reserved areas of the light box surface, for entering commands and uncertainty codes. When the pen is pressed in these areas the +program responds accordingly. Each time the pen tip is depressed in the digitizing area the program sounds the bell on the terminal to indicate to the user that a point has been recorded. As the sequence is read the program displays it on the screen. +\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 1.4\tab Introduction to editing single sequences\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The editing method used by the programs is designed to give users access to an editor with which they are familiar - i.e. the one on their machine, say EDT on a VAX or EMACS on a UNIX system, and yet to allow them to edit a sequence which contains all the +landmarks they need in order to know where they are. Users can create a file containing a simple listing of the sequence (single stranded) with numbering, using "list the sequence", and then edit it with their syste +m editor, using the numbering to know where they are within the sequence. When the edits are complete they exit from the editor and the program "analyses" the edited file to extract only the sequence characters. Similarly a file containing a three phase tr +anlslation, or a file containing a sequence plus its three phase translation, plus its restriction sites marked above the sequence (see figure 3.1), can be edited. In order to be able to "analyse" such complicated listings and correctly extract the sequenc +e the following simple rule is used\: + all lines in the file that contain a character that is not A,C,T,G or U are deleted. It is obviously important to be aware of this rule and its implications. For protein sequences only a simple listing i.e. the sequence plus numbering, can be used.\par +\pard\plain \s6\sb160\sa60\sl280\tx560\tx860 \b\f20 1.5\tab Introduction to using the sequence libraries\par +\pard\plain \s4\qj\sa120\sl280 \f20 The installation of the sequence libraries is described in the introductory chapter. Direct access to the libraries is provided by all programs that need such a facility\: it is + not performed by separate programs. The facilities currently offered in NIP, PIP, SIP, NIPL, PIPL, and SIPL include the following\:\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 \tab Get a sequence by knowing its entry name\par +\tab Get a sequences' annotation by knowing its entry name\par +\tab Get an entry name by knowing its accession number\par +\pard\plain \li1120\ri1240\sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth \f4\fs16 HapII\par +\pard \li1120\ri1240\sl220\brdrl\brsp100\brdrth \brdrr\brsp100\brdrth HpaII\par + MspI MseI\par +. .HincII\par +. .HindII\par +. .HpaI DsaV\par +. .. EcoRII\par +. .. TspAI\par +. .. . ApyI\par +. .. . BstNI\par +. .. . MvaI\par +. .. . ScrFI MaeIII\par +. .. . . . BsrI MseI\par +ccggttagactgttaacaacaaccaggttttctactgatataactggttacatttaacgc\par + 10 20 30 40 50 60\par + P V R L L T T T R F S T D I T G Y I * R\par + R L D C * Q Q P G F L L I * L V T F N A\par +\pard \li1120\ri1240\sl220\keepn\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth G * T V N N N Q V F Y * Y N W L H L T P\par +\pard\plain \s8\qj\fi-1140\li1140\sb80\sa120\sl240\tx1140 \f21\fs20 Figure 3.1\tab The first page width of a sequence display that can be edited by the program.\par +\pard\plain \s7\qj\fi-560\li560\sb360\sa120\sl280\tx560 \f20 \tab Search the author index for author names\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \tab Search the freetext index for keywords\par +\pard\plain \s4\qj\sa120\sl280 \f20 The facilities currently offered in NIPL, PIPL and SIPL include\:\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 \tab Search whole library\par +\tab Search only a list of entry names\par +\tab Search all but a list of entry names\par +\pard\plain \s5\sa60\sl320\tx560 \b\f20\fs28 2.\tab Methods\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.1\tab Sequence input from keyboard\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Type in gel readings".\par +2.\tab Accept "Use special keys for A,C,T,G".\par +3.\tab Define the keys in turn.\par +4.\tab Define "File file names". A file of file names so the readings can be processed as a batch.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Define in the sequence by typing it in using the selected keys. Finish by typing an @ symbol.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Define "File name for this gel reading". This is the name for the sequence just entered.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Accept "Type in another reading". This cycles round to step 5. If rejected the next step follows.\par +8.\tab Accept "List gel readings". The batch of readings entered will each be listed, one after the other, headed by their file names, on the screen.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.2\tab Sequence input from digitizer\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Tape the autoradiograph down securely on the light box.\par +2.\tab Start the program (GIP).\par +3.\tab Define "File of file names".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Using the digitizer pen hit the digitizer menu ORIGIN, program menu ORIGIN, program menu START.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \tab After the bell has sounded the program will give the default lane order. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab If correct hit CONFIRM otherwise hit RESET. To reset the lane order hit the A,C,G,T boxes in the menu in left to right order.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Hit START, then hit in left to right order, at a height level with the first band to be read, the start positions for the next four lanes. The progr +am will report the mean lane separations and asks for confirmation that they are correct.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Hit START\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 8.\tab Hit the bands on the film in sequence order. If necessary use the uncertainty codes in the program menu. Continue until the sequence is finished.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 9.\tab Hit STOP.\par +10.\tab Define "Name for this reading".\par +11.\tab Accept "Read another sequence". Otherwise the program will stop.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.3\tab Sequence input from the Pharmacia A.L.F.\par +\pard\plain \s4\qj\sa120\sl280 \f20 After processing and base calling on the PC the data for all 10 clones is contained in a single f +ile, and the user names each using local conventions. Then this single file is transfered to the SUN using PC-NFS. This program allows SUN directories to be mounted as if they were DOS disks and data can be transfered by use of the DOS copy command. On th +e SUN, to prepare for processing by program XBAP the 10 clones are split into 10 separate files each with the names given on the PC. In addition a file of file names is written Then the reads for the individual clones need to be examined to clip off the v +ector sequence and the poor data at the 5' end. See note 2.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.4\tab Sequence input from the ABI 373A.\par +\pard\plain \s4\qj\sa120\sl280 \f20 After processing and base calling on the Macintosh the data for each clone is contained in 2 files\: + one is simply the sequence but the main file contains the raw data, trace data and sequence. For our processing we do not use the sequence file as we can ex +tract all we need from the main file. The user names each file using local conventions and then the folder is transfered to the SUN using TOPS. This program +allows SUN directories to be mounted as if they were on the Macintosh and data can be transfered by simply dragging folders on the Macintosh screen. On the SUN, to prepare for processing by program XBAP, a file of file names is written and the reads for t +he individual clones are examined to clip off the vector sequence and the poor data at the 5' end. See note 2.\par +\pard\plain \s6\fi-560\li560\sb240\sa120\sl280\tx560\tx980 \b\f20 2.5\tab Editing a nucleic acid sequence using restriction sites and a translation and base numbering as landmarks.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select NIP.\par +2.\tab Read in the sequence to be edited.\par +3.\tab Direct output to disk, say creating file edit.seq.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Use the restriction enzyme site search routine (See the relevant chapter) to create a file showing "Names above the sequence", as in figure 3.1.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Close the redirection file.\par +6.\tab Select "Edit the sequence". \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Define "Name of file to edit". This is the file containing the sequence listing, say edit.seq.The sytem editor will start up.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 8.\tab Edit the sequence.\par +9.\tab Exit from the editor.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 10.\tab Accept "Make edited sequence active". The edited sequence will replace the original sequence. \par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.6\tab Searching the freetext (or author) index of a sequence library\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Read new sequence".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Select "Sequence library". The alternative is "Personal file", and if taken would be followed by questions about which of the formats "Staden, EMBL, GenBank, PIR, GCG or FASTA" it was stored in.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Select, say, "EMBL nucleotide library".\par +4.\tab Select "Search text index for keywords".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Define "Keywords". Type up to 5 keywords separated by spaces - i.e.space is the delimiting character (see note below about author searches).\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab +The search will start and for each match the program will display the contents of the matching line which includes the entry name, primary accession number, its length and a 80 character description. After every 20 matches the program will ring the bel +l and the user can escape by typing "!".\par +\tab The commands for searching the author index are effectively the same. Note that for authors it is useful to be able to link words together for names s +uch as De Gaule or von Meyenberg. The symbol underscore (_) can be used for this purpose - e.g. De_Gaule or von_meyenberg. The same facility is available for the keyword searches.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.7\tab Using accession numbers to retrieve data from a sequence library\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Read new sequence".\par +2.\tab Select "Sequence library".\par +3.\tab Select, say, "EMBL nucleotide library".\par +4.\tab Select "Get entry names from accession numbers".\par +5.\tab Define "Accession number". \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab The program will display the entry names corresponding to the accession number. The last entry name found will become the default entry name.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.8\tab Displaying the annotations for an entry in a sequence library\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Read new sequence".\par +2.\tab Select "Sequence library".\par +3.\tab select, say, "EMBL nucleotide library".\par +4.\tab Select "Get annotations".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Define "Entry name". The program will display the annotation for the entry. After every 20 lines the program will ring the bell and the user can escape by typing "!".\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.9\tab Reading a sequence from a sequence library\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Read new sequence".\par +2.\tab Select "Sequence library".\par +3.\tab Select, say, "EMBL nucleotide library".\par +4.\tab Select "Get a sequence".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Define "Entry name". The program will make the sequence the active sequence and display its base composition.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.10\tab Worked example of sequence library access\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The worked example in figure 3.2 shows a search of the text index for the keywords p53 and mouse, followed by a search of the author index for the names sanger and coulson, followed by search on accession number v00636, followed by "Get annotatio +ns" for entry lambda, and finally "Get a sequence" for entry lambda. \par +\pard\plain \sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth \f4\fs16 {\f22\fs18 Select sequence source\par +}\pard \sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth {\f22\fs18 X 1 Personal file\par + 2 Sequence library\par + ? Selection (1-2) (1) =2\par + Select a library\par + X 1 EMBL 29 nucleotide library Dec 91\par + 2 SWISSPROT 20 protein library Nov 91\par + 3 PIR 31 protein library Dec 91\par + 4 NRL3D 58 From Brookhaven protein library Dec 91\par + 5 GenBank example\par + ? Selection (1-5) (1) =\par +Library is in EMBL format with indexes\par + Select a task\par + X 1 Get a sequence\par + 2 Get annotations\par + 3 Get entry names from accession numbers\par + 4 Search author index\par + 5 Search text index for keywords\par + ? Selection (1-5) (1) =5\par + Search for keywords\par + ? Keywords=p53 mouse\par +P53 hits 73\par +MOUSE hits 10140\par +\'00\par + MMANT01 X00875 536 Murine gene fragment for cellular tumour antigen\par + MMANT02 X00876 83 Murine gene fragment for cellular tumour antigen\par + MMANT03 X00877 21 Murine gene fragment for cellular tumour antigen\par + MMANT04 X00878 261 Murine gene fragment for cellular tumour antigen\par + MMANT05 X00879 184 Murine gene fragment for cellular tumour antigen\par + MMANT06 X00880 113 Murine gene fragment for cellular tumour antigen\par + MMANT07 X00881 110 Murine gene fragment for cellular tumour antigen\par + MMANT08 X00882 137 Murine gene fragment for cellular tumour antigen\par +}\pard \sl220\brdrl\brsp100\brdrth \brdrr\brsp100\brdrth {\f22\fs18 MMANT09 X00883 74 Murine gene fragment for cellular tumour antigen\par + MMANT10 X00884 107 Murine gene for cellular tumour antigen p53 (exon\par + MMANT11 X00885 562 Murine p53 gene 3' region with exon 11\par + MMANTP53 M26862 536 Mouse tumor antigen p53 gene, 5' end.\par + MMLYN M64608 2044 Mouse lyn protein mRNA, complete cds.\par + MMP53 X00741 1377 Mouse mRNA for transformation associated protein\par + MMP53A M13872 1285 Mouse p53 mRNA, complete cds, clone pcD53.\par + MMP53B M13873 1241 Mouse p53 mRNA, complete cds, clone p53-m11.\par + MMP53C M13874 1322 Mouse p53 mRNA, complete cds, clone p53-m8.\par + MMP53G1 X01235 554 Mouse genomic DNA for 5' region of cellular tumou\par + MMP53IN4 X60470 729 M.musculus p53 gene for p53 protein, intron 4\par +\'00\par + MMP53P X01236 2132 Mouse pseudogene for cellular tumour antigen p53\par + MMP53R X01237 1773 Mouse mRNA for cellular tumour antigen p53\par + MMRSB2P5 M64597 196 Mouse B2 repeat in the 3' flank of protein 53 (p5\par + MMSFFV1 X64656 165 M.musculus Friend spleen focus forming virus (SFF\par + MMSFFV2 X64657 142 M.musculus Friend spleen focus forming virus (SFF\par + 24 different entries found\par +\'00\par + Select a task\par + X 1 Get a sequence\par + 2 Get annotations\par + 3 Get entry names from accession numbers\par + 4 Search author index\par + 5 Search text index for keywords\par + ? Selection (1-5) (1) =4\par + Search for keywords\par + ? Keywords=coulson sanger\par +COULSON hits 935\par +SANGER hits 15\par +\'00\par + LAMBDA V00636 48502 Genome of the bacteriophage lambda (Styloviridae)\par + MIBTXX V00654 16338 Complete bovine mitochondrial genome.\par + MIHSCG J01415 16569 Human mitochondrion, complete genome.\par + MIHSM1 M10546 2771 Human mitochondrial DNA, fragment M1, encoding tr\par + MIHSXX V00662 16569 H.sapiens mitochondrial genome\par + MIPX1C01 M10860 130 Bacteriophage phi-X174, nucleotides 3920-4049.\par + MIPX1C02 M10861 115 Bacteriophage phi-X174, nucleotides 3480-3595.\par + MIPX1C03 M10862 121 Bacteriophage phi-X174, nucleotides 4260-4380.\par + MIPX1CTI M10849 130 Bacteriophage phi-X174, nucleotides 3389-3520.\par + PHIX174 V01128 5386 Bacteriophage phi-X174 (cs70 mutation) complete g\par + R17CPRAA M24826 61 Bacteriophage R17 coat protein RNA fragment.\par + 11 different entries found\par +\'00\par + Select a task\par + X 1 Get a sequence\par + 2 Get annotations\par + 3 Get entry names from accession numbers\par + 4 Search author index\par + 5 Search text index for keywords\par + ? Selection (1-5) (1) =3\par + ? Accession number=v00636\par +Entry name LAMBDA\par + Select a task\par + X 1 Get a sequence\par + 2 Get annotations\par + 3 Get entry names from accession numbers\par + 4 Search author index\par + 5 Search text index for keywords\par + ? Selection (1-5) (1) =2\par + Default Entry name=LAMBDA\par + ? Entry name=\par +ID LAMBDA standard; DNA; PHG; 48502 BP.\par +}\pard \sl220\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth {\f22\fs18 XX\par +AC V00636; J02459; M17233; X00906;\par +XX\par +DT 09-JUN-1982 (Rel. 01, Created)\par +DT 03-JUL-1991 (Rel. 28, Last updated, Version 3)\par +XX\par +DE Genome of the bacteriophage lambda (Styloviridae).\par +XX\par +KW circular; coat protein; DNA binding protein; genome;\par +KW origin of replication.\par +XX\par +OS Bacteriophage lambda\par +OC Viridae; ds-DNA nonenveloped viruses; Siphoviridae.\par +XX\par +RN [1]\par +RP 1-48502\par +RA Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B.;\par +RT "Nucleotide sequence of bacteriophage lambda DNA";\par +RL J. Mol. Biol. 162\:729-773(1982).\par +XX\par +\'00\par + Select a task\par + X 1 Get a sequence\par + 2 Get annotations\par + 3 Get entry names from accession numbers\par + 4 Search author index\par + 5 Search text index for keywords\par + ? Selection (1-5) (1) =\par + Default Entry name=LAMBDA\par + ? Entry name=\par +DE Genome of the bacteriophage lambda (Styloviridae).\par + Sequence length 48502\par + Sequence composition\par + T C A G -\par + 11988. 11360. 12336. 12818. 0.\par +}\pard \sl220\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth {\f22\fs18 24.7% 23.4% 25.4% 26.4% 0.0%\par +}\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 3.2\tab A worked example of sequence library use.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 3.\tab NOTES\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab +The program menu for GIP is simply a set of boxes drawn on the digitizing surface that each contain a command or uncertainty code. Right handed users will find it is best to position the menu to the right of the digitizing +area, but in practice as long as its top edge is parallel to the digitizer box, it can be put anywhere in the active region. As well as the codes a,c,g,t,1,2,3,4,b,d,h,v,r,y,x,-,5,6,7,8 the following commands are included in the menu\: + DELETE removes the la +st character from the sequence; RESET allows the lane centres to be redefined; START means begin the next stage of the procedure; STOP means stop the current stage in the procedure; CONFIRM means confirm that the last command or set of coordinates are corr +ect. \par +\tab +The digitizing device also has a menu of its own. This lies in a two inch wide strip immediately in front of the digitizing box. Pen positions within this two inch strip are interpretted as commands to the digitizer and are not sent to the GIP program. In + general the only time users will need to use the device menu is when they tell GIP where the program menu lies in the digitizing area. This is done by first hitting ORIGIN in the device menu and then hitting the bottom left hand corner of the progra +m menu. If the bell does not sound after hitting START try hitting METRIC in the device menu (the program uses metric units, and some digitizers are set to default to use inches; hitting metric switches between the two).\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \tab +The user should try to hit the bands as near as possible to the centre of the lanes because the program tracks the lanes up the film using the pen positions. If the lane centres get too close the program stops responding to the pen positions of bands and +hence does not ring the bell. If t +his occurs users must hit the reset box in the menu and the program will request them to redefine the lane centres at the current reading position. Then they can continue reading. As a further safeguard the program will only respond to pen positions either + in the menu or very close to the current reading position.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Details about preparing the data from fluorescent sequencing machines for processing by XBAP are contained in the notes for the chapter on managing sequencing projects. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab All of the operation +s described for the EMBL nucleotide library can be performed in exactly the same way for GenBank and the SWISSPROT and PIR protein libraries. For keyword searching the freetext index is most useful because it contains all words in feature tables, definiti +on lines, title lines, keywords and comment lines. The searches are very fast. The search will find all words that start with the given keywords\: + e.g. keyword sugar will match with sugar, sugaractivating, sugars, etc. When several keywords are used together, only entries indexed on all the words will be reported. On the VAX, EMBL, GenBank, SWISSPROT and PIR can all be processed. \par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 4.\tab References\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Staden, R. 1984. A computer program to enter DNA gel reading data into a computer. {\i Nucl. Acids Res}. {\b 12}, 499-503.\par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 4. Managing Sequencing Projects\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +2.\tab Methods\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.1\tab Starting a project database\par +2.2\tab Screening against restriction enzyme recognition sequences\par +2.3 \tab Screening against vector sequences\par +2.4 \tab Entering readings in to the project database (assembly)\par +2.5\tab Searching for internal joins\par +2.6\tab Editing in XBAP\par +2.7\tab Joining contigs interactively in XBAP\par +2.8\tab Selecting primers and templates\par +2.9\tab Examining the quality of a consensus\par +2.10\tab Using graphical displays to examine contigs\par +2.11\tab Disassembling contigs\par +2.12\tab Shuffling pads\par +2.13\tab Displaying a contig\par +2.14\tab Highlighting differences between readings and the consensus\par +2.15\tab Screen editing contigs in SAP\par +2.16\tab Automatic editing in SAP\par +2.17\tab Using the original editor in SAP\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Notes\par +4.\tab References\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 +Data input, assembly, checking and editing are the major tasks of sequence project management. Data input is described in a previous chapter and here we cover everything else. The programs can deal with data derived from autoradiographs and from automated +gel reading machines such as the Applied Biosystems 373A and the Pharmacia A.L.F. and film readers such as the Amersham scanner \par +\pard \s4\qj\sa120\sl280 We describe two alternative programs for managing sequencing projects. They contain the same assembly and vector screen +ing routines but they differ in their editing methods. One program SAP (see references 1 and 2) can be operated from simple terminals and emulators but the other XBAP (3) requires an X terminal or emulator. XBAP contains a superior editor plus the facility + to annotate sequences and display the coloured traces for data derived from fluorescent sequencing machines. Those using autoradiographs will find that SAP is adequate but XBAP is essential for users of fluorescent sequencing machines. Readers should note + that several of the methods for displaying contigs described below are probably of value only to those unable to use the screen based contig editor in XBAP.\par +\pard \s4\qj\sa120\sl280 +Fluorescent sequencing machines provide machine readable data. This means, given appropriate software, that while making editing decisions the user can see, displayed on the screen, the coloured traces used to derive the sequence. However data from these +machines requires some extra processing. First the machines tend to produce long sequences with po +or quality at their 3' ends and so we have to decide how much of the data to use. Secondly the sequencing machine does not recognise the primer region (as the user would) so we need to have some way of removing it from the data. The poor quality data from +both ends of the sequence and the vector sequences are identified non-interactively by programs clip-seqs and vep. Alternatively these tasks can be performed interactively using program TED (4). We term the data from the 3' end of a reading that is not emp +loyed in the assembly process "unused" sequence. Note that we do not lose this data but simply ignore it until such time as it can be useful for locating joins between contigs, or for double stranding regions of the sequence.\par +\pard \s4\qj\sa120\sl280 +The method described here uses a database to store all the data for each sequencing project. The individual sequence readings derived from autoradiographs or from sequencing machines are initially stored in separate files but the program copies them into t +he database during the assembly process. For normal operation the program handles batches of readings - say 24 from a film or machine run. Batch processing is achieved by use of files of file names. \par +\pard \s4\qj\sa120\sl280 Depending on the strategy employed and the stage of the project the following operations may be performed.\par +\pard\plain \s7\qj\fi-560\li560\sb100\sa120\sl280\tx560 \f20 1)\tab Start a project database.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2)\tab Select primers and templates.\par +3)\tab Obtain readings.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4)\tab Put individual readings into the computer and write a file of file names. For data derived from fluorescent sequencing machines choose which data from + the 3' end of the reading should not be used for the assembly process.\par +5)\tab Screen the batch against any vectors that may be present, excising any vector sequence found and passing to the next step, the names of those readings that contain some non-vector sequence.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6)\tab Screen the batch against any restriction sites whose presence would indicate a problem, passing those that do not match on to the next step.\par +7)\tab Compare each reading in the batch with the current contents of the project database adding them to the contigs they overlap, joining contigs or starting new contigs.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 8)\tab +Check the number of contigs and the quality of the consensus sequence and plan further experiments. Try to join contigs by searching for overlaps between their ends. (This is particularly useful for those using data from fluorescent sequencing machines, + where although the 3' end of the sequence is not good enough for automatic assembly, it can be valuable for finding overlaps between contigs).\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 9)\tab Edit the contigs to resolve dissagreements.\par +10)\tab Produce a consensus sequence.\par +11)\tab Analyse the consensus sequence, possibly discovering further errors.\par +\pard\plain \s4\qj\sa120\sl280 \f20 +Subsets of these operations will be cycled through repeatedly. A pure shotgun strategy would continue using steps 3-7, a pure primer walking strategy would also include step 2. A number of the steps require almost no user intervention, however checking qua +lity and final editing decisions are still interactive procedures. The program contains several options, such as displays of the overlapping reading +s in a contig, to help indicate, not only the poorly determined regions, but also which clones could be resequenced to resolve ambiguities, or those which can usefully be extended or sequenced in the reverse direction, to cover difficult regions. It is bes +t to use a command procedure or script for handling steps 5-7.\par +\pard \s4\qj\sa120\sl280 For our projects we have a script which users employ by typing "assemble filename", where filename is the file of file names for the current batch of readings. This script calls all the necessa +ry options in SAP or BAP (see notes) in order to make a backup of the database, screen against any vectors, assemble readings and print a report. In the text below we describe how these operations are performed interactively. \par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 2.\tab Methods\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.1\tab Starting a project database\par +\pard\plain \s4\qj\sa120\sl280 \f20 The assembled data for each project is stored in a database. At the beginning of a project it is necessary to create an empty database using program SAP or XBAP.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Open database"\par +2.\tab Select "Start new database"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Define the database name. Database names can have from one to 12 letters and must not include full stop (.). \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Accept "Database is for DNA"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab +Define "Database size". This is an initial size and if necessary can be increased later using "Copy database". Roughly speaking it is the number of readings expected to be needed to complete the project. Currently BAP limits the maximum to 8000 and SAP + has a limit of 1000.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Define "Maximum reading length". This is the length of the longest reading that will be added to the database. The minimum is 512 bases, and the maximum 4096.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The program should confirm that "copy 0" of the database has been started. See Note 14 for important information.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.2\tab Screening against restriction enzyme recognition sequences\par +\pard\plain \s4\qj\sa120\sl280 \f20 +For some strategies it is necessary to compare readings against any restriction enzyme recognition sequences that may have been used during cloning and which should not be present in the data. The function operates on single readings or processes batches a +ccessed through files of file names. The algorithm looks for exact matches to recognition sequences. The recognition sequences should be stored in a simple text file with one recognition sequence per record.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Accept "Use file of filenames".\par +2.\tab Define "File of gel reading names". The input file of file names.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +Define "File for names of sequences that pass". A file of file names for those readings that do not contain the recognition sequences. After the run it will contain the names of all the files in the batch that do not match any + of the restriction enzyme recognition sequences. Hence it can be used for further processing of the batch.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "File name of recognition sequences". The name of the file of recognition sequences.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.3\tab Screening against vector sequences\par +\pard\plain \s4\qj\sa120\sl280 \f20 +For most strategies it is necessary to compare readings against any vector sequences that may have been picked up during cloning. The package contains two routines for screening against vectors. The original function simply reports any matches between the +readings and t +he vector sequences and only passes on those that do not match. This function should now only be used to screen for any other sequences that should be excluded from the database, because the newer one (program name VEP for vector excising program) is capab +le of both finding the vector sequences and editing them out automatically. \par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.3.1\tab Clipping off vector sequences\par +\pard\plain \s4\qj\sa120\sl280 \f20 There are two types of vector that may need to be screened out of gel readings\: the sequencing vector and, for cases where, say, whole cosmids +have been shotgunned, the cloning vector. The two tasks are different. When screening out the sequencing vector we may expect to find data to exclude, both from the primer region and from the other side of the cloning site (when, for example, the insert i +s short). When screening out cosmid vector we may find that either the 5' end, or the 3' end, or the whole of the sequence is vector. Also for the cosmid search we need to compare both strands of the sequence. The program (VEP) works slightly differently f +or each of the two cases. Having read the vector sequence from a file the program asks for the "Position of the cloning site". A value of zero signifies that the search will be for the cosmid vector. A nonzero value signifies that the search is for the seq +uencing vector, and so in this case the program then asks for the "Relative position of the primer site". A negative relative position signifies that a reverse primer is being used, otherwise a forward primer is assumed.\par +\pard \s4\qj\sa120\sl280 The program screens a batch of read +ings using a file of file names and creates a new file of file names which contains the names of all those sequences that include some nonvector sequence. For each sequence that contains some vector it writes out a new copy of the file in which the vector +portion is identified.\par +\pard \s4\qj\sa120\sl280 +The search, which uses a hashing algorithm, is very rapid. Users specify a "Word length", the "Number of diagonals to combine" and a "Minimum score". The word length is the minimum number of consecutive bases that will count as a mat +ch. The algorithm treats the problem like a dot matrix comparison and finds the diagonal with the highest score. Then it adds the scores for the adjacent "Minimum number of diagonals to combine". If the combined score is at least "Minimim score" the sequen +ce is marked to indicate that it contains vector. The score represents the proportion of a diagonal that contains matching words, so the maximum score for any diagonal is 1.0.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Define "Input file of file names". This is the file containing the names of all the readings to be screened.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Define "File name of vector sequence". \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +Define "Position of cloning site". This is the base number, relative to the beginning of the vector sequence, that is on the 3' side of the insert site. For example for m13mp18 the SmaI site is at 6249. A zero value signifies that the search is for cosm +id vector.\par +4.\tab Define "Relative position of 3' end of primer site". This is the position, relative to the cloning site, of the first base that could be included in the sequence. For m13mp18, the 17mer Sequencing Primer and the SmaI site, the position is 41. +\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Define "Word length". Only words of this length will be counted as matches.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Define "Number of diagonals to combine". The scores for this number of diagonals around the highest scoring diagonal will be combined to give the total score.\par +7. \tab Define "Cutoff score". For a match, at least this proportion of the total length of the summed diagonals must contain identical words. \par +8.\tab Define "Output file of passed file names". The name of the file to contain the names of the readings to pass on to the assembly program.\par +\pard\plain \s4\qj\sa120\sl280 \f20 Processing will commence and finishes with a summary stating the number of files processed, the number completely vector, the number partly vector and the number free of vector.\par +\pard\plain \s9\fi-560\li860\sb160\sa60\sl280\tx1140 \b\f20 2.3.2\tab Screening for "vectors"\par +\pard\plain \s4\qj\sa120\sl280 \f20 This function is contained in both SAP and XBAP and operates on single readings or processes batches accessed through files of file names. The algorithm looks for exact matches of length "minimum match length" and disp +lays the overlapping sequences.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Accept "Use file of filenames".\par +2.\tab Define "File of gel reading names". The input file of file names.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +Define "File for names of sequences that pass". A file of file names for those readings that do not contain the vector sequence. After the run it will contain the names of all the files in the batch that do not match the vector sequence. Hence it can be + used for further processing of the batch.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "File name of vector sequence". The name of the file containing the vector sequence.\par +\pard\plain \s6\sb160\sa60\sl280\tx560\tx860 \b\f20 2.4\tab Entering readings into the project database (Assembly)\par +\pard\plain \s4\qj\sa120\sl280 \f20 +Readings are entered into the database using the auto assemble function. This function compares each reading and its complement with a consensus of all the readings already stored in the database. If it finds any overlaps it aligns the overlapping sequence +s by inserting padding characters, and then adds the new reading to the database. Readings that overlap are added to existing contigs and readings that do not overlap any data in +the database start new contigs. If a new reading overlaps two contigs they are joined. Any readings that appear to overlap but which cannot be aligned sufficiently well are not entered and have their names written to a file of failed gel reading names. Not +e that it is possible that a reading may align well with two contigs (indicating a possible join) but that after it has been added to one of the contigs, the two contigs do not align sufficiently well. In this case, although the reading has been entered in +to the database its name will also be added to the file of failed readings. Alignments using more than the maximum number of paddings characters, or exceeding the maximum mismatch may be displayed, but the readings will not be entered into the database. It + is advisable to set the consensus cutoff to 51% before running the assembly routine as this will improve the alignments. A typical run of the assembly routine is shown in figure 4.1.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Accept "Permit entry"\par +2.\tab Accept "Use file of file names"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Define "File of gel reading names". The name of the input file of file names, probably passed on from "Screen against vector".\par +4.\tab Define "File for names of failures". A file to contain the names of the readings that the program fails to enter, or for which joins are not made.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Select "Perform normal shotgun assembly"\par +6.\tab Accept "Permit joins"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Define "Minimum initial match". Only possible overlaps containing exact matches of at least this number of consecutive identical characters will be considered for alignment.\par +8.\tab Define "Maximum number of pads per reading" This is the maximum number of padding characters permitted in any new reading during the alignment procedure\par +9.\tab Define "Maximum number of pads per reading in contig" This is the maximum number of padding characters permitted in the contig in order to align any new reading.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 10.\tab Define "Maximum percent mismatch after alignment"\par +\pard\plain \li560\ri500\sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth \f4\fs16 Automatic sequence assembler\par +\pard \li560\ri500\sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth Database is logically consistent\par +? (y/n) (y) Permit entry\par +? (y/n) (y) Use file of file names\par +? File of gel reading names=demo.nam\par +? File for names of failures=demo.fail\par +Select entry mode\par +X 1 Perform normal shotgun assembly\par + 2 Put all sequences in one contig\par + 3 Put all sequences in new contigs\par +? Selection (1-3) (1) =\par +? (y/n) (y) Permit joins\par +? Minimum initial match (12-4097) (15) =\par +? Maximum pads per gel (0-25) (8) =\par +? Maximum pads per gel in contig (0-25) (8) =\par +? Maximum percent mismatch after alignment (0.00-15.00) (8.00) =\par +\par +Results skipped to save space\par +\par +>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>\par +Processing 4 in batch\par +Gel reading name=hinw.009 \par +Gel reading length= 292\par +Working\par +Contig 1 position 263 matches strand 1 at position 14\par +Contig 2 position 1 matches strand 1 at position 156\par +\pard \li560\ri500\sl220\brdrl\brsp100\brdrth \brdrr\brsp100\brdrth Total matches found 2\par +Trying to align with contig 1\par +Padding in contig= 1 and in gel= 0\par +Percentage mismatch after alignment = 2.9\par +Best alignment found\par + 251 261 271 281\par + aattacagcg tt,cctattg acgggcgcat ccac\par + ********** ** ** **** ********** ****\par + aattacagcg ttcccvattg acgggcgcat ccac\par + 1 11 21 31\par +Trying to align with contig 2\par +Padding in contig= 0 and in gel= 2\par +Percentage mismatch after alignment = 1.4\par +Best alignment found\par + 1 11 21 31 41 51\par + tgcacgacat cgagtatgag agttatatcc cgggcgcgct ctgcttgtac atggacctca\par + ********** ********** ********** ********** ********** **********\par + tgcacgacat cgagtatgag agttatatcc cgggcgcgct ctgcttgtac atggacctca\par + 156 166 176 186 196 206\par + 61 71 81 91 101 111\par + tgtacctctt tgtctccgtg ctctacttca tgccctccga gcccggcagc gcccacactg\par + ********** ********** ********** ********** ***** ** * **********\par + tgtacctctt tgtctccgtg ctctacttca tgccctccga gcccg,ca,c gcccacactg\par + 216 226 236 246 256 266\par + 121 131\par + ctcagacgac ggtcgctgc\par + ********** *********\par + ctcagacgac ggtcgctgc\par + 276 286\par +Overlap between contigs 2 and 1\par +Length of overlap between the contigs= -122\par +Entering the new gel reading into contig 1\par +This gel reading has been given the number 4\par +Working\par +Trying to align the two contigs\par +Padding in contig= 2 and in gel= 0\par +Percentage mismatch after alignment = 1.5\par +Best alignment found\par + 406 416 426 436 446 456\par + tgcacgacat cgagtatgag agttatatcc cgggcgcgct ctgcttgtac atggacctca\par + ********** ********** ********** ********** ********** **********\par + tgcacgacat cgagtatgag agttatatcc cgggcgcgct ctgcttgtac atggacctca\par + 1 11 21 31 41 51\par + 466 476 486 496 506 516\par + tgtacctctt tgtctccgtg ctctacttca tgccctccga gcccg,ca,c gcccacactg\par + ********** ********** ********** ********** ***** ** * **********\par + tgtacctctt tgtctccgtg ctctacttca tgccctccga gcccggcagc gcccacactg\par + 61 71 81 91 101 111\par + 526 536\par + ctcagacgac ggtcgct\par + ********** *******\par + ctcagacgac ggtcgct\par + 121 131\par +Editing contig 1\par +\pard \li560\ri500\sa100\sl220\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth Completing the join between contigs 1 and 2\par + (Results for other readings skipped to save space)\par +\pard \li560\ri500\sl220\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth Batch finished\par + 9 sequences processed\par + 9 sequences entered into database\par +\pard \li560\ri500\sa100\sl220\keepn\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth 2 joins made\par +\pard\plain \s8\qj\fi-1140\li1140\sb60\sa120\sl240\tx1140 \f21\fs20 Figure 4.1\tab Part of a typical run of "Auto assemble".\par +\pard\plain \s6\sb160\sa60\sl280\tx560\tx860 \b\f20 2.5\tab Searching for internal joins \par +\pard\plain \s4\qj\sa120\sl280 \f20 +The purpose of this function is to use data already in the database to find possible joins between contigs. Although most joins will be made automatically during assembly, due to poor alignments, some may not have been done. The function is particularly us +eful for sequences from fluorescent sequencing machines because it may be possible to find potential joins within the unused data from the 3' ends of readings. For each potential + join found, when the X version is used, the contig joining editor is automatically called up with the two contigs aligned in the edit windows.\par +\pard \s4\qj\sa120\sl280 +The program strategy is as follows. Take the first contig and calculate its consensus. If unused data is being employed, examine all readings that are in the complementary orientation, and sufficiently near to the contigs left end, to see if they have suff +iciently good unused sequence which, if present, would protrude from the left end of the contig. If found add th +e longest such sequence to the left end of the consensus. Do the same for the right end by examining readings that are in their original orientation. Repeat the consensus calculations and extensions for all contigs hence producing an extended consensus for + the whole database. If unused data is not being employed simply calculate the consensus for the whole database. Now look for possible joins by processing the extended consensus in the following way. Take the last, say 500, bases (termed the "probe length" + by the program) of the rightmost consensus, compare it in both orientations with the extended consensus of all the other contigs. Display any sufficiently good alignments. Repeat with the left end of the rightmost contig. Do the same for the ends of all t +he contigs, always comparing only with the contigs to their left, so that the same matches do not appear twice. \par +\pard \s4\qj\sa120\sl280 Good unused data is defined by sliding a window of "Window size for good data scan" bases outwards along the sequence and stopping when greater + than "Maximum number of dashes in scan window" appear in the window. Note that it is advisable to have some sort of cutoff because if we simply take all the data it might be of such poor quality that we wont find any good matches. An initial run employing + no unused data is also recommended. Sufficiently good alignments are defined by criteria equivalent to those used in auto assemble, however here we only display alignments that pass all tests.\par +\pard \s4\qj\sa120\sl280 All numbering is relative to base number one in the contig\: ma +tches to the left (i.e. in the unused data) have negative positions, matches off the right end of the contig (i.e. in the unused data) have positions greater than the contig length. The convention for reporting the orientations of overlaps is as follows\: + i +f neither contig needs to be complemented the positions are as shown. If the program says "contig x in the - sense" then the positions shown assume contig x has been complemented. For example in the results given in figure 4.2 the positions for the first o +verlap are as reported, but those for the second assume that the contig in the minus sense (i.e. 443) has been complemented.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Find internal joins".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Define "Minimum initial match". Only matches containing this number of consecutive identical characters will be found.\par +3.\tab Define "Maximum pads per sequence". Only alignments containing less than or equal this number of padding characters in each sequence will be found.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Maximum percent mismatch after alignment". Only alignments with at lea +st this level is similarity will be found. Particularly when poor data from the 3' ends of sequences derived from fluorescent sequencing machines is used, it is important to allow for a high degree of mismatch - say around 75%.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Define "Probe length". This is the size of sequence from each end of each contig, that is compared with the total length of all other contigs.\par +6.\tab Accept "Employ unused data". This means, where available, add the unused data from the 3' ends of sequences, to the ends of the contigs.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab +Define "Window size for good data scan". To decide how much of the unused data should be added to the end of a contig the program scans outwards, counting the numbers of dashes (-) over a window of the size defined here.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 8.\tab Define "Number of dashes in scan window". If the program finds this many dashes in the scan window it will add no more of the unused data to the end of the contig.\par +\pard\plain \qj\li680\ri780\sl220\pagebb\box\brsp100\brdrth \f4\fs16 Possible join between contig 445 in the + sense and contig 405\par +\pard \li680\ri780\sl220\box\brsp100\brdrth Percentage mismatch after alignment = 4.9\par + 412 422 432 442 452 462\par +405 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAG,TT AGCTCACTCA\par + ********* * ******** ***** *** ********** ********** **********\par +445 -TTCCCGACT G,AAAGCGGG TAGTGA,CGC AACGCAATTA ATGTGAG-TT AGCTCACTCA\par + -127 -117 -107 -97 -87 -77\par + 472 482 492 502 512\par +405 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT AT\par + ********** ********** ********** ********** **\par +445 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT AT\par + -67 -57 -47 -37 -27\par +Possible join between contig 443 in the - sense and contig 423\par +Percentage mismatch after alignment = 10.4\par + 64 74 84 94 104 114\par +423 ATCGAAGAAA GAAAAGGAGG AGAAGATGAT TTTAAAAATG AAACG-CGAT GTCAGATGGG\par + **** ***** ********** ********** ****** ** ***** **** *********\par +443 ATCG,AGAAA GAAAAGGAGG AGAAGATGAT TTTAAA,,TG AAACGACGAT GTCAGATGG,\par + 3610 3620 3630 3640 3650 3660\par + 124 134 144 154 164\par +423 TTG-ATGAAG TAGAAGTAGG AG-AGGTGGA AGAGAAGAGA GTGGGA\par + *** ****** ********** ** ******* *** ***** ** **\par +443 TTGGATGAAG TAGAAGTAGG AGGAGGTGGA ,GAG,AGAGA GTTGG-\par +\pard \li680\ri780\sl220\keepn\box\brsp100\brdrth 3670 3680 3690 3700 3710\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 4.2\tab Typical output from "Find internal joins".\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.6\tab Editing in XBAP\par +\pard\plain \s4\qj\sa120\sl280 \f20 The XBAP editor is mouse-driven and can insert, delete and change readings in contigs. It has facilities to display the traces for data from fluorescent sequenci +ng machines and for annotation of readings. In addition it allows the poor quality data from the ends of readings to be viewed and, if required, added to the sequences. \par +\pard \s4\qj\sa120\sl280 +A typical view of the editor is shown in figure 4.3. This includes the edit window showing an 80 character section of a contig, (position 3899 to 3978). Each reading is numbered and named in the left hand panel, minus signs indicating those in their revers +e orientation. Underneath is their consensus. Some of the sequence letters are lighter + than the majority showing that they are "unused". One segment (3933 to 3949) is shaded which signifies that it has been annotated. The editing cursor is at position 3921. Above this window are the main buttons the user employs to direct the editing proces +s. Below the edit window is a panel showing the traces for readings 37 and 123. Notice they are centred on the cursor position. Here the traces are shown in four different line styles, but on a colour screen they each have different colours. In the bottom +of the figure is the search window. These features are described in the relevant sections below.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.6.1\tab Scrolling through the contig\par +\pard\plain \s4\qj\sa120\sl280 \f20 The editor allows scrolling from one end of a contig to the other using the scroll bar and scroll buttons and also the arrow keys.\par +\pard \s4\qj\sa120\sl280 Action of mouse button presses when the mouse pointer is in the scroll bar\:\par +\pard \s4\qj\li1720\sa120\sl280\tx4520 Middle Mouse Button\tab Set editor position\par +Left Mouse Button\tab Scroll forward one screenful\par +Right Mouse Button\tab Scroll backwards one screenful\par +\pard\plain \li80\ri20\sl220\keepn\box\brsp20\brdrth \f4\fs16 {{\pict\macpict\picw444\pich344 +82daffffffff015701bb1101a0008201000affffffff015701bb0900000000000000003100000000015601ba98007e00000000030703e900000000030703e900000000015601ba000102830002830002830007000286aa01a00007000186550140000700028600012000070001860001400007000286000120000b02013ff8 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+0012fe00010401fb000321000002c0000010d40012fe00010201fb000321000002c0000008d40012fe00010401fb000321000002c0000010d40012fe00010201fbff03e1fffffec0000008d4000afe000004b5000010d4000afe000002b5000008d4000afe000004b5000010d4000afe000002b5000008d4000afe000004b5 +000010d4000afe000002b5000008d4000afe000004b5000010d4000afe000002b5000008d4000afe000005b5550050d4000afe000002b5aa00a8d400028300028300028300028300028300028300028300028300028300028300028300a00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 4.3\tab A typical display from the contig editor in XBAP\par +\pard\plain \s4\qj\sb160\sa120\sl280 \f20 The four scroll buttons operate as follows\:\par +\pard \s4\qj\li1720\sa120\sl280\tx4520 "<<"\tab Scroll left half a screenful\par +"<"\tab Scroll left one character\par +">"\tab Scroll right one character\par +">>"\tab Scroll right half a screenful\par +\pard \s4\qj\sa120\sl280 +The Editor cursor can be positioned anywhere in the edit window by moving the mouse pointer over the character of interest, then pressing the left mouse button. The Editor cursor can also be moved by using the direction arrow keys.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.6.2\tab Editing operations \par +\pard\plain \s4\qj\sa120\sl280 \f20 The editor operates in two main edit modes - Replace + and Insert. Replace allows a character to be replaced by another. Insert allows characters to be inserted into a reading. Characters are entered by typing them from the keyboard. Only valid characters are permitted. Characters can be deleted by positionin +g the cursor one character to their right, then pressing the delete key. Normally Insert and Delete apply to the consensus line of the contig only. This restraint can be overridden by using the "Super Edit" mode of operation, though it should be employed w +ith caution as misuse may corrupt alignments.\par + +Edits can also be performed on the consensus, though they are restricted to insertion and deletion of padding characters ("*"). These edits also have special meanings. A deletion will delete all characters at the position to the left of the cursor in the c +ontig, and move the relative positions of all sequences starting to the right of the cursor position left one character. An insertion will insert the character typed ("*") into all gel reading sequences at the +cursors position in the contig, and move the relative positions of all sequences starting to the right of the cursor position right one character.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.6.3\tab Use of buttons \par +\pard\plain \s4\qj\sa120\sl280 \f20 The effect of the last edit can be undone by pressing the "Undo" button at the top of the editor window. Pressing it n times will undo the last n edits.\par +\pard \s4\qj\sa120\sl280 The cursor will automatically be positioned at the next problem when the "Find Next Problem" button is selected. The next problem is where the consensus shows either a disagreement ("-") or a pad ("*") character.\par +\pard \s4\qj\sa120\sl280 The edits to the contig can be saved by pressing the "Leave Editor" button and replying "Yes" to the prompt to "Save changes?".\par +As no changes are made to the working copy of the database until this point it is possible to abort the editor if the edit session ends up in an unsatisfactory state.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.6.4\tab Displaying traces for readings from fluorescent sequencing machines\par +\pard\plain \s4\qj\sa120\sl280 \f20 The original trace data from which the gel reading sequences were derived can be seen by double clicking (two quick clic +ks) with the middle mouse button on the area of interest. The trace will be displayed with the point clicked at the centre of the trace viewport. All traces that are displayed are maintained in one window, which will display a maximum of four traces. When +four traces are already being displayed and a new one is requested, the one at the top of the window is removed and the new one is added to the bottom. Traces can be removed individually by using the "quit" button in the panel next to the trace. \par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.6.5\tab Extending reads with the unused data\par +\pard\plain \s4\qj\sa120\sl280 \f20 +Sequence data from fluorescent sequencing machines is normally clipped to remove the primer region and the poor quality data from the 3' end is marked to be ignored during assembly. Only the sequence used during assembly is made visible in the XBAP editor. + However the unused data is copied into the database and can be viewed from within the editor. Also the position of this "cutoff" can be altered. To display the unused sequences, press the "Display Cutoff" button at the to +p of the editor window. The cutoff sequence appears in grey. This sequence can be incorporated into the editable sequence, by moving the cutoff position. This is done by positioning the cursor at the end of the sequence, and using Meta-Left-Arrow and Meta- +Right-Arrow to adjust the point of cutoff. The Meta key is a diamond on the Sun keyboard.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.6.6\tab Using the pop-up menu\par +\pard\plain \s4\qj\sa120\sl280 \f20 A pop-up menu is revealed by depressing the "Control" key on the keyboard and at the same time pressing the left mouse button.\par +\pard \s4\qj\sa120\sl280 The menu has the following functions\:\par +\pard\plain \li1880\sl220 \f4\fs16 Find Next Problem\par +Highlight Disagreements\par +Save Contig\par +Create Tag\par +Edit Tag\par +Delete Tag\par +Search\par +Select Oligo\par +\pard\plain \s4\qj\sa120\sl280 \f20 \par +\pard \s4\qj\sa120\sl280 "Find Next Problem" and "Save Contig" are described above. Operations on tags are described in the section on annotation below, and then searching is outlined.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.6.7\tab Annotating readings\par +\pard\plain \s4\qj\sa120\sl280 \f20 Parts of a sequence can be annotated to record the positions of primers used for walking, or to mark sites, such as compressions, that have caused problems during sequencing. The annotations ar +e termed "tags". Each tag has a type such as "primer", a position, a length and a comment. Each type has an associated colour that will be shown on the display. First the segment to tag is selected, then it is annotated. The consensus sequence cannot be a +nnotated.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.6.8\tab Creating a new annotation\par +\pard\plain \s4\qj\sa120\sl280 \f20 Use the left mouse button to position the start of the selection. While this button is being held down, move the mouse to the other end of the segment. The selection can be extended further using the right mouse bu +tton. To create the annotation, invoke the pop-up menu, and select the "Create Tag" function. A small "tag editor" will appear which allows users to select the type of the annotation from a pull-down menu, and specify a comment if desired. To select a new +type pull down the Type menu, and select the entry desired. To enter a comment, simply type into the text window in the tag editor. The annotation is created when the "Leave" button on the tag editor is pressed, and is displayed in the colour defined in th +e tag database file (TAGDB).\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.6.9\tab Editing an existing annotation\par +\pard\plain \s4\qj\sa120\sl280 \f20 +Position the cursor with the left mouse button on the tag, and select the "Edit Tag" off the pop-up menu. This invokes the tag editor, and changes to the type and comment of the annotation can be made. The tag is updated when the "Leave" button is pressed. +\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1180 \b\f20 2.6.10\tab Deleting an annotation\par +\pard\plain \s4\qj\sa120\sl280 \f20 To delete an existing annotation, position the cursor with the left mouse button on the tag, and select the "Delete Tag" off the pop-up menu.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1160 \b\f20 2.6.11\tab Searching\par +\pard\plain \s4\qj\sa120\sl280 \f20 +Selecting "Search" brings up a window which can remain present during normal editor operation. The window allows the user to select the direction of search, the type of search and a value to search on. The value is entered into a value text window, then pr +essing the "search" button performs the search. If successful, the cursor is positioned accordingly. An audible tone indicates failure. Pressing the "ok" button removes the search window. The search window is automatically removed when the contig editor is + exited. There are seven different search modes.\par +\pard\plain \s9\fi-560\li1120\sb180\sa60\sl280\tx1700 \b\f20 2.6.11.1\tab Search by position\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This positions the cursor at the numeric position specified in the value text window. Eg a value of "1234" causes the cursor to be placed at base number 1234 in the contig. Positioning withing a reading is achieved by prefixing the number with the "@" char +acter, eg "@123" positions the cursor at base 123 of the sequence in which the cursor lies. Relative positions can be specified by prefixing the number with a plus or minus charac +ter. Eg "+1234" will advance the cursor 1234 bases. If possible, the cursor is positioned within the same sequence. The direction buttons have no effect on the operation of "search by position".\par +\pard\plain \s9\fi-560\li1120\sb180\sa60\sl280\tx1720 \b\f20 2.6.11.2\tab Search by reading name\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This positions the cursor at the left end of the gel reading specified in the value text window. If the value is prefixed with a slash it is assumed to be a gel reading name. Otherwise it is assumed to be a gel reading number. Eg "123" positions the cursor + at the left end of gel readi +ng number 123. "/a16a12.s1" positions at the start of reading a16a12.s1. If the value was "/a16" the cursor is positioned at the first reading which starts with "a16". The direction buttons have no effect on the operation of "search by reading name". +\par +\pard\plain \s9\fi-560\li1120\sb180\sa60\sl280\tx1700 \b\f20 2.6.11.3\tab Search by tag type\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This positions the cursor at the start of the next tag which has the the same type as specified by the type value menu. To change the type, select from the menu that pops up when the mouse is clicked on the button labeled "Type\:". Th +e search can be performed either forwards or backwards from the current cursor position. To find all tags, use "search by annotation", with a null text value string.\par +\pard\plain \s9\fi-560\li1120\sa60\sl280\tx1700 \b\f20 2.6.11.4\tab Search by annotation\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This positions the cursor at the start of the next tag which has a comment containing the string specified in the value text window. The search performed is a regular expression search, and certain characters have special meanings. Be careful when your val +ue string contains ".", "*", "[", "^" or "$". The search can be performed either forwards or backwards from the current cursor position.\par +\pard\plain \s9\fi-560\li1120\sa60\sl280\tx1700 \b\f20 2.6.11.5\tab Search by sequence\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This positions the cursor at the start of the next piece of sequence that matches the value specified in the text value window. The search is for an exact match, which means that the case of the value string is important. The search is performed on the gel + readings themselves, rather than the consensus sequence. The search can be performed either forwards or backwards from the current cursor position.\par +\pard\plain \s9\fi-560\li1120\sa60\sl280\tx1700 \b\f20 2.6.11.6\tab Search by problem\par +\pard\plain \s4\qj\sa120\sl280 \f20 This positions the cursor at the next place in the consensus sequence which is not "A", "C", "G" or "T". The search can be performed either forwards or backwards from the current cursor position.\par +\pard \s4\qj\sa120\sl280 \par +\pard\plain \s9\fi-560\li1120\sa60\sl280\tx1700 \b\f20 2.6.11.7\tab Search by quality\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This positions the cursor at the next place in the consensus sequence where the consensus for each strand is not "A", "C", "G" or "T" or where the two strands disagree. The search can be performed either forwards or backwards from the current cursor posit +ion.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 \par +2.7\tab Joining contigs interactively using XBAP\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The operation of the join editor in XBAP is very similar to the one for single contigs described above. It allows the user to align the ends of the two contigs by editing each contig separately. First specify which two contigs are to be joined. The program + checks that the two contig numbers are different (it will not allow circles to be formed!) The Join Editor consists of two Contig Editors in between which is sandwiched a disagreement box. This disagreement box + uses exclamation marks to denote mismatches between the two consensuses. A typical example is shown in figure 4.4. Here we see in the top window the right end of one contig and in the bottom window the left end of another. The left end of the overlap is c +orrectly aligned, as indicated by an absense of exclamation marks, but the top contig has an extra character at position 558 which is spoiling the alignment over the next segment. Notice that the "lock" button is highlighted denoting that the user has aske +d for the two contigs to scroll together.\par +\pard \s4\qj\sa120\sl280 The best strategy for joining is to align the leftmost character of the right contig with its counterpart in the left contig. Then press the \'d2Lock\'d3 + button before editing the contigs to make them align for the whole overlap. The overlap must be of at least +one character. Use the scroll bar and the scroll buttons ("<<", "<", ">", and ">>") for positioning the relative positions of the two contigs. The join position can be fixed by pressing the "lock" button at the top + of the Join Editor. Locking allows the two contigs to be scrolled as one when using the scroll bar and buttons, the left ends always in the same position relative to each other. Once locked, it is best to proceed to the right along the contigs, inserting +padding characters ("*") into the consensuses to minimise the disagreements. It is important that the user aligns the two contigs throughout the whole region of overlap before completing the join because it is only at this stage that the two contigs can be + edited independently. If a join is completed leaving a region of mismatch the consensus will consist of dashes and the assembly function will fail to find overlaps in the bad section. Misaligned sections can be corrected using the "super edit" mode of the + editor. The join can be completed by pressing the "Leave Editor" button. The percentage mismatch is displayed, and users are required to confirm that they want to perform the join.\par +\pard\plain \li100\ri80\sb100\sl220\keepn\box\brsp20\brdrth \f4\fs16 {{\pict\macpict\picw441\pich144 +4685ffffffff008f01b81101a0008201000affffffff008f01b80900000000000000003100000000008e01b798007c00000000014003db00000000014003db00000000008e01b7000102850002850026e600001ff9ff0087f8ff01f87ff5ff01fe1fefff0087fcff01fe1ffcff01f87ff2ff00e0f40026e600001ff9ff0084 +f80001087ff5ff01fe10ef000084fc0001021ffcff01f840f2000020f40026e600001ff9ff0084f80001087ff5ff01fe10ef000084fc0001021ffcff01f840f2000020f40026e600001ff9ff0084f80001087ff5ff01fe10ef000084fc0001021ffcff01f840f2000020f4003701003cfa000203fc03fa0008630c18000181 +80001ff9ff0084f80001087ff5ff01fe10ef000084fc0001021ffcff01f840f2000020f4005b010066fa0002030003fa000ac30c38000380c0001f807ffbff05841f8000003cfd0002087f87fbff07e01fe7fffffe107efc00030f000078fd00133c0f0000841860000600021f9ffffffe7ff84180fb00021fe018fc000020 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+0078618301e0301e0780c0781e078618780c02000b0010ed00000c9d000102000b0010ed00000c9d000102000b0010ed00000c9d000102000b0010ed00000c9d000102000b0010ed00000c9d000102000b0010ed00000c9d000102000b0010ed00000c9d000102000b0010ed00000c9d000102000b0010ed00000c9d000102 +0007001f88ff01fe00028500028500a00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 4.4\tab A typical display from the join editor in XBAP.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.8\tab Selecting primers and templates\par +\par +\pard\plain \qj \f4\fs16 {\plain \f20 1. Select "Edit contig". The primer and template selection function is available from the popup menu of the contig editor.\par +}\pard \qj {\plain \f20 \par +}\pard \qj {\plain \f20 2. Open the oligo selection window, by selecting "Select Oligo" from the contig editor popup menu.\par +}\pard \qj {\plain \f20 \par +}\pard \qj {\plain \f20 3. Position the cursor to where you want the oligo to be chosen. While the oligo selection window is visible, you will still have complete control over positioning and editing within the contig editor.\par +}\pard \qj {\plain \f20 \par +}\pard \qj {\plain \f20 4. Indicate the strand for which you require an oligo. This is done by toggling the direction arrow ("----->" or "<------"), if necessary.\par +}\pard \qj {\plain \f20 \par +}\pard \qj {\plain \f20 +5. Press the "Find Oligos" button to find all suitable oligos (See "Oligo selection" in Note 17.) Information for the closest oligo to the cursor position is given in the output text window. In the contig editor the position of the oligo is marked by a + temporary tag on the consensus. The window is recentered if the oligo is off the screen. Selecting "Display Selection Information" will print a short report on the numbers of oligos considered and rejected during oligo selection. \par +}\pard \qj {\plain \f20 \par +}\pard \qj {\plain \f20 6. If this oligo is not suitable (it may have been previously chosen, and found to be unsuitable by experimentation, say), the next closest oligo can be viewed by pressing "Select Next". \par +}\pard \qj {\plain \f20 \par +}\pard \qj {\plain \f20 +7. Suitable templates are automatically identified for the currently displayed oligo (See "Template selection" in Note 18.) By default, the template is that closest to the oligo site. If the choice is not suitable (it may be known to be a poor quality +template, say) another can be chosen from the "Choose Template for this Oligo" menu. Templates that do not appear on the menu can be specified by selecting "other". However, the template must be on the correct strand and be upstream of the oligo. \par +}\pard \qj {\plain \f20 \par +}\pard \qj {\plain \f20 +8. A tag can be created for the current oligo by pressing the button "Create a tag for this oligo". The annotation for this tag holds the name of the template and the oligo primer sequence. There are fields to allow the user to specify their own primer + name ("serial#") and comments ("flags") for this tag. An example of oligo tag annotation\: \par +}\pard \qj {\plain \f20 \par + serial#= \par + template=a16a9.s1 \par + sequence=CGTTATGACCTATATTTTGTATG \par + flags=\par +\par +}\pard \qj {\plain \f20 9. The oligo selection window is closed when "Create a tag for this oligo" or "Quit" is selected. \par +}\pard \qj {\plain \f20 \par +}\pard\plain \s6\qj\sa60\tx560\tx860 \b\f20 \par +\pard \s6\sa60\sl280\tx560\tx860 2.9\tab Examining the "quality" of a contig\par +\pard\plain \s4\qj\sa120\sl280 \f20 This function reports on the proportion of the consensus that is "well determined" and will display a sequence of symbols that indicate the quality +of the consensus at each position or produce a graphical display. Each strand of the contig is analysed separately using the consensus algorithm, and a position is declared "well determined" if it is assigned one of the symbols a,c,g,t. The current consen +sus calculation cutoff score is used.\par +\pard \s4\qj\sa120\sl280 A summary showing the percentage of the consensus that falls into each category of quality is shown. The analysis divides the data into five categories, assigning each a code as shown in figure 4.5. Code 0 means well +determined on both strands and they agree, 1 means well determined on the plus strand only, 2 means well determined on the minus strand only, 3 means not well determined on either strand and 4 means well determined on both strands but they disagree. If +the user chooses to have the data displayed graphically the following scheme is used. A rectangular box is drawn so that the x coordinate represents the length of the contig. The box is notionally divided vertically into 5 possible levels which are given t +he y values\: + -2,-1,0,1,2. The quality codes assigned to each base position are plotted as rectangles. Each rectangle represents a region in which the quality codes are identical, so a single base having a different code from its immediate neighbours will a +ppear as a very narrow rectangle. Obviously a single line at the midheight shows a perfect sequence. In figure 4.6 we show the result for the section of contig shown in figure 4.8.\par +\pard \s4\qj\sa120\sl280 \par +\par +\par +\par +\par +\pard \s4\qj\li1580\ri1760\sb160\sl280\box\brsp100\brdrth \tqc\tx2000\tqc\tx3960\tqc\tx6360 \tab {\b Strands\tab Quality\tab Y cordinates\par +}\pard \s4\qj\li1580\ri1760\sa120\sl280\box\brsp100\brdrth \tqc\tx2000\tqc\tx3960\tqc\tx6200 {\b \tab OK\tab code\par +}\pard \s4\qj\li1580\ri1760\sa120\sl280\box\brsp100\brdrth \tqc\tx1780\tqc\tx2120\tx2380\tqc\tx3960\tqr\tx6000\tx6220\tqr\tx6740 \tab +\tab -\tab and the same\tab 0\tab 0\tab to\tab 0\par +\pard \s4\qj\li1580\ri1760\sa120\sl280\box\brsp100\brdrth \tqc\tx1780\tqc\tx2120\tqc\tx3960\tqr\tx6000\tx6220\tqr\tx6740 \tab +\tab \tab 1\tab 0\tab to\tab 1\par +\tab -\tab \tab 2\tab -1\tab to\tab 0\par +\pard \s4\qj\li1580\ri1760\sa120\sl280\box\brsp100\brdrth \tqc\tx2120\tqc\tx3960\tqr\tx6000\tx6220\tqr\tx6740 \tab neither\tab 3\tab -1\tab to\tab 1\par +\pard \s4\qj\li1580\ri1760\sa60\sl280\keepn\box\brsp100\brdrth \tqc\tx1780\tqc\tx2120\tx2400\tqc\tx3960\tqr\tx6000\tx6220\tqr\tx6740 \tab +\tab -\tab but different \tab 4\tab -2\tab to\tab 2\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 4.5\tab The codes and coordinates used by the "Quality plot". \par +\par +\pard\plain \li1500\ri1660\sb400\sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth \f4\fs16 94.67 % OK on both strands and they agree(0)\par +\pard \li1500\ri1660\sl220\brdrl\brsp100\brdrth \brdrr\brsp100\brdrth 0.67 % OK on plus strand only(1)\par + 2.00 % OK on minus strand only(2)\par + 2.67 % Bad on both strands(3)\par + 0.00 % OK on both strands but they disagree(4)\par +\pard \li1500\ri1660\sl220\brdrl\brsp100\brdrth \brdrr\brsp100\brdrth {\fs22 \par +}\pard \li1500\ri1660\sl220\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth 3310 3320 3330 3340 3350\par +0000000000 0000000000 0000000000 0000000000 0000000000\par +\par + 3360 3370 3380 3390 3400\par +0020000000 0000000032 0000032000 0000000000 0300000030\par +\par + 3410 3420 3430 3440 3450\par +\pard \li1500\ri1660\sl220\keepn\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth 0000000000 0010000000 0000000000 0000000000 0000000000\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 4 6\tab Listed output from "Examine Quality" showing the results for the section of contig displayed in figure 4.8.\par +\pard \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.10\tab Using graphical displays to examine contigs\par +\pard\plain \s4\qj\sa120\sl280 \f20 The programs contain three graphical displays to aid the examination of contigs. The first simply gives an overview of all the contigs in the database and provides, with the use of a +crosshair, a mechanism for the other two displays to select contigs. One of these displays produces a schematic representation of each of the readings in a contig. The lines in the display show the relative positions of each reading and also their sense. T +he plot is divided vertically into two sections by a line that is identified by an asterisk drawn at each end. All lines that lie above this line represent readings that are in their original sense, all lines below show readings that are in the complementa +ry sense. The final graphical display is of the "quality" of the data as described above.\par +\pard \s4\qj\sa120\sl280 +When these graphical displays are visible users may employ a crosshair, moved by mouse or keyboard commands, to examine the data in more detail. The crosshair is positioned and when keyboard characters S, Q, N or Z are typed the program will show the local + aligned sequences in a text window, produce the quality plot, give the names of the nearest readings or zoom into the display. \par +\pard \s4\qj\sa120\sl280 A typical display of all three plots +is shown in figure 4.7. The top rectangle shows a separate line for each of the projects contigs. The righthand one is bisected by a vertical line indicating that it has been selected by the user. The next rectangle below is divided by a horizontal line ma +rked at each end by an asterisk. Each of the other horizontal lines in the box represents one of the selected contigs gel readings. Those above the dividing line are in their original orientation, those below have been complemented. The box below is also d +ivided by a horizontal line and shows the "quality" for each base in the contig. Rectangluar areas marked above the central line show sections that only have a good consensus on the minus strand, and rectangles below show good sections from the other stran +d. Places where the vertical lines reach the top and bottom of the box show disagreements between the two strands. Places with only the midline have a good consensus on both strands.\par +\pard\plain \li80\sl220\keepn\tx720 \f4\fs16 {{\pict\macpict\picw441\pich231 +237effffffff00e601b81101a0008201000affffffff00e601b8090000000000000000310000000000e501b79800780000000001f103bb0000000001f103bb0000000000e501b70001028900028900028900028900028900090100158e550054ff000901000f8eff00f0ff000d0100089b000008f5000010ff000d0100089b +000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b00 +0008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b000008f5000010ff000d0100089b0000 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+0163f0fe000301000040fe0000e0fc000010fe0002400410ff004c03000852b8fc00022800c8e9000020fc00026c0040ef00010180fc001240002400018808001041770000010434000820fd000163f0fe000301000040fe0000e0fc000010fe0002400410ff004a03000852b8fc00022fffc8e9000020fc00026c0040ef00 +010180fc001240002400018808001041770000010434000820fd000163f0fe000301000040fe0000e0fc00001ffcff00f0ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010 +ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1 +000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc00 +0004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff0019010008f8000008ce000001f30000 +10fc000004e1000010ff0019010008f8000008ce000001f3000010fc000004e1000010ff000901000f8eff00f0ff00028900028900028900028900028900028900a00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 4.7\tab A typical graphical display from XBAP or SAP.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 \par +2.11\tab Disassembling contigs\par +\pard\plain \s4\qj\sa120\sl280 \f20 +Sometimes it is necessary to drastically alter contigs. We may need to break a contig in two, remove a single reading, remove a whole set of consecutive readings from a contig, or remove a set of readings from the database independent of which contigs they + are in. \par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.11.1\tab Removing a single reading\par +\pard\plain \s4\qj\sa120\sl280 \f20 This function is found in the "Alter relationships" menu. The user types in the number of the reading to be removed. If the reading is required to hold the contig together - i.e. is the only one cove +ring a particular region - the program will create an extra contig consisting of the data to the right of the removed reading. The original contig will be shortened accordingly.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.11.2\tab Removing a set of readings\par +\pard\plain \s4\qj\sa120\sl280 \f20 This function is called "Disassemble readings" and can remove any group of readings from a database. It works in two modes\: + 1. A set of adjacent readings in a contig can be removed by the user naming the two end ones (the left one first); 2. A set of readings from any number of contigs can be remove +d by the user giving the name of a file that contains their names. In both modes the program cleans up the database by moving data to fill up any holes made in the files.\par + + For both modes of operation the program request a file of file names. If the user creates their own file (i.e. mode 2) each reading name must be on a separate line of the file. For mode 1 the user names the leftmost then the rightmost reading for removal. + They MUST be in left to right order. They and all intervening readings will be removed. For both modes, if necessary, new contigs will be created. \par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.11.3\tab Breaking a contig\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This function is found in the "Alter relationships" menu. It can be used to break a contig at the beginning of a particular reading so that the identified reading becomes the left end of a new contig. The user types in the number of the reading that will b +ecome the left end.\par +\pard \s4\qj\sa120\sl280 \par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.12\tab Shuffling pads\par +\pard\plain \s4\qj\sa120\sl280 \f20 One weakness of the assembly routine is that padding characters introduced to line up the readings are not always aligned with the pads in other sequences\: + a single problem such as a compression can give rise to pads apparently randomly arranged in the different readings covering the region. This function attempts to shuffle the pads around so that they align with one another, h +ence simplifying editing. No information is lost in the process\: only the positions of padding characters are changed. The function is best used prior to editing.\par +\pard\plain \s6\sb160\sa60\sl280\tx560\tx860 \b\f20 2.13\tab Displaying a contig\par +\pard\plain \s4\qj\sa120\sl280 \f20 The "Display a contig" option shows the aligned readings for any par +t of a contig. Users select "Display a contig", then select the contig. The number, name and strandedness of each reading is shown and the consensus is written below. A typical example, showing part of a contig from positions 3301 to 3450, is seen in figu +re 4.8. Overlapping this region are readings 3, 40, 8, 37, 35 and 2, with archive names L3.SEQ, A21A7.S1 and so on. Readings 3, 8, 35 and 2 are in reverse orientation as indicated by the minus signs. There are a few padding characters in the working versio +ns, but the consensus (shown below each page width) has a definite assignment for every position except 3376. \par +\pard\plain \s6\sb160\sa60\sl280\tx560\tx860 \b\f20 2.14\tab Highlighting differences between readings and the consensus\par +\pard\plain \s4\qj\sa120\sl280 \f20 +During the latter stages of a project this option is used to highlight disagreements between individual gel readings and their consensus sequences. Typical output is seen in the figure 4.9 which shows the result for the section of contig shown in figure 4. +8. Characters that agree with the consensus are shown as + symbols for the plus +strand and - for the minus strand. Characters that disagree with the consensus are left unchanged and so stand out clearly. Note that a similar display is now more conveniently available within the contig editor.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Set the consensus cutoff score.\par +2.\tab Redirect output to disk.\par +3.\tab Display the contig.\par +4.\tab Close the redirection file.\par +5.\tab Select "Highlight disagreements".\par +6.\tab Define the name of the redirection file.\par +7.\tab Define an output file name.\par +8.\tab Select a symbol for good plus strand data.\par +9.\tab Select a symbol for good minus strand data.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \page \par +\pard\plain \li760\ri760\sl220\box\brsp100\brdrth \tqr\tx8240 \f4\fs16 10.\tab Print the file.{\plain \f20 \par +}\pard \li760\ri760\sl220\box\brsp100\brdrth \tqr\tx8240 \tab 3310 3320 3330 3340 3350\par +\pard \li760\ri760\sl220\box\brsp100\brdrth -3\tab L3.SEQ \tab atggttacgccagactatcaaatatgctgcttgaggcttattcgggcgca\par +40\tab A21A7.S1\tab atggttacgccagactatcaaatatgctgcttgaggcttattcgggcgca\par +-8\tab A16A2.S1\tab atggttacgccagactatcaaatatgctgcttgaggcttattcgggcgca\par +37\tab A21A2.S1\tab atggttacgccagactatcaaatatgctgcttgaggcttattcgggcgca\par +\tab CONSENSUS\tab atggttacgccagactatcaaatatgctgcttgaggcttattcgggcgca\par +\par +\tab 3360 3370 3380 3390 3400\par +-3\tab L3.SEQ\tab gatctgaccaagcgacag*tttaaa*gtgctgcttgccatt*ctgcgt*a\par +40\tab A21A7.S1\tab gatctgaccaagcgacag*gttaaagttgctgctt\par +-8\tab A16A2.S1\tab gatctgaccaagcgacag*tttaaa*gtgctgcttgccatt*ctgcgt*a\par +37\tab A21A2.S1\tab ga-ctgaccaagcgacag*tttaaa*gtgctgcttgccatt*ctgcgt*a\par +35\tab A16D12.S1\tab gttttaaa-gtgctgcttgccatttctgcgtaa\par +-2\tab L2.SEQ\tab t*ctgcgt*a\par +\tab CONSENSUS\tab gatctgaccaagcgacag*tttaaa-gtgctgcttgccatt*ctgcgt*a\par +\par +\tab 3410 3420 3430 3440 3450\par +-3\tab L3.SEQ\tab aaacctatgggt*ggaataaaccaatggacagaatcaccgattctcaact\par +-8\tab A16A2.S1\tab aaacctatgggt*ggaataaaccaatggacagaatcaccgattctcaact\par +37\tab A21A2.S1\tab aaacctatgggtgggaataaaccaatggacagaatcaccgattctcaact\par +35\tab A16D12.S1\tab aaacctatgggt*ggaataaaccaatggacagaatcaccgattctcaact\par +-2\tab L2.SEQ\tab aaacctatgggt*ggaataaaccaatggacagaatcaccgattctcaact\par +\pard \li760\ri760\sl220\box\brsp100\brdrth \tab CONSENSUS\tab aaacctatgggt*ggaataaaccaatggacagaatcaccgattctcaact\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 4.8\tab Typical output from "Display contig".\par +\pard\plain \li840\ri940\sb320\sl220\box\brsp100\brdrth \f4\fs16 3310 3320 3330 3340 3350\par +\pard \li840\ri940\sl220\box\brsp100\brdrth -3 L3.SEQ --------------------------------------------------\par + 40 A21A7.S1 ++++++++++++++++++++++++++++++++++++++++++++++++++\par + -8 A16A2.S1 --------------------------------------------------\par + 37 A21A2.S1 ++++++++++++++++++++++++++++++++++++++++++++++++++\par + atggttacgccagactatcaaatatgctgcttgaggcttattcgggcgca\par +\par + 3360 3370 3380 3390 3400\par + -3 L3.SEQ -------------------------*------------------------\par + 40 A21A7.S1 +++++++++++++++++++g+++++gt++++++++\par + -8 A16A2.S1 -------------------------*------------------------\par + 37 A21A2.S1 ++-++++++++++++++++++++++*++++++++++++++++++++++++\par +-35 A16D12.S1 -t----------------------t------a-\par + -2 L2.SEQ ----------\par + gatctgaccaagcgacag*tttaaa-gtgctgcttgccatt*ctgcgt*a\par +\par + 3410 3420 3430 3440 3450\par + -3 L3.SEQ --------------------------------------------------\par + -8 A16A2.S1 --------------------------------------------------\par + 37 A21A2.S1 ++++++++++++g+++++++++++++++++++++++++++++++++++++\par +-35 A16D12.S1 --------------------------------------------------\par + -2 L2.SEQ --------------------------------------------------\par +\pard \li840\ri940\sl220\keepn\box\brsp100\brdrth aaacctatgggt*ggaataaaccaatggacagaatcaccgattctcaact\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 \par +\pard \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 Figure 4.9\tab Typical output from "Highlight disagreements", showing the results for the section of contig displayed in figure 4.8.\par +\pard \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.15\tab Screen editing contigs in SAP\par +\pard\plain \s4\qj\sa120\sl280 \f20 When using SAP the best way for users to edit a whole contig interactively is to use their prefered external editor on the standard display of a contig. When the screen edit function is selected SAP writ +es a text file containing a display of the contig and passes it to an external editor - say EDT on the VAX or emacs on a UNIX system. The user modifies the file using the editor and when the editor is exited SAP moves the changed contig back into the proje +ct database.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Screen edit".\par +2.\tab Select the contig to edit.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Define a temporary file for use by the editor. After a slight pause the editor will start and the first page of the contig will appear on the screen.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Edit the contig using the editors standard commands.\par +5.\tab Exit from the editor.\par +6.\tab Accept "Put contig back into the database".\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.16\tab Automatic editing of contigs in SAP\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This function automatically changes characters in gel readings to make them agree with the consensus sequence. At first sight this may seem like an unethical procedure but as is explained in the notes it is quite legitimate and saves a great deal of time. +In figure 4.10 we show the effect on using autoedit on the section of contig displayed in figure 4.8. All changed characte +rs (for example position 3369, reading A21A7.S1) are denoted by uppercase letters. Note that apart from position 3375 which has an unresolved consensus all other changes have been made. These edits were made using a combined consensus for both strands, but + the standard version of the program treats each strand separately and will only make a change if the consensus for the two strands agree.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Redirect output to disk.\par +2.\tab Select "Display contig".\par +3.\tab Identify the contig to edit/display.\par +4.\tab Close the redirection file.\par +5.\tab Print the file containing the displayed contig.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Check the contig and the original films and annotate the printout to indicate the required edits.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Set the cutoff for the consensus calculation.\par +8.\tab Select "Auto edit".\par +9.\tab Identify the contig and the section to edit. \par +10.\tab The program will display a summary of changes made.\par +11.\tab Display the contig and compare it with the annotated printout.\par +12.\tab Use another editing method to finish the editing.\par +\pard\plain \li820\ri960\sl220\pagebb\box\brsp100\brdrth \f4\fs16 3310 3320 3330 3340 3350\par +\pard \li820\ri960\sl220\box\brsp100\brdrth -3 L3.SEQ atggttacgccagactatcaaatatgctgcttgaggcttattcgggcgca\par + 40 A21A7.S1 atggttacgccagactatcaaatatgctgcttgaggcttattcgggcgca\par + -8 A16A2.S1 atggttacgccagactatcaaatatgctgcttgaggcttattcgggcgca\par + 37 A21A2.S1 atggttacgccagactatcaaatatgctgcttgaggcttattcgggcgca\par + CONSENSUS atggttacgccagactatcaaatatgctgcttgaggcttattcgggcgca\par +\par + 3360 3370 3380 3390 3400\par + -3 L3.SEQ gatctgaccaagcgacagtttaaa*gtgctgcttgccattctgcgtaaaa\par + 40 A21A7.S1 gatctgaccaagcgacagTttaaagGtgctg\par + -8 A16A2.S1 gatctgaccaagcgacagtttaaa*gtgctgcttgccattctgcgtaaaa\par + 37 A21A2.S1 gaTctgaccaagcgacagtttaaa*gtgctgcttgccattctgcgtaaaa\par +-35 A16D12.S1 gtttaaa-gtgctgcttgccattctgcgtaaaa\par + -2 L2.SEQ tctgcgtaaaa\par + CONSENSUS gatctgaccaagcgacagtttaaa-gtgctgcttgccattctgcgtaaaa\par +\par + 3410 3420 3430 3440 3450\par + -3 L3.SEQ cctatgggtggaataaaccaatggacagaatcaccgattctcaacttag\par + -8 A16A2.S1 cctatgggtggaataaaccaatggacagaatcaccgattctcaacttagc\par + 37 A21A2.S1 cctatgggtggaataaaccaatggacagaatcaccgattctcaacttagc\par +-35 A16D12.S1 cctatgggtggaataaaccaatggacagaatcaccgattctcaacttagc\par + -2 L2.SEQ cctatgggtggaataaaccaatggacagaatcaccgattctcaacttagc\par +\pard \li820\ri960\sl220\keepn\box\brsp100\brdrth CONSENSUS cctatgggtggaataaaccaatggacagaatcaccgattctcaacttagc{\fs22 \par +}\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 4.10\tab The result of applying the "Auto editor" to the section of contig displayed in figure 4.5.\par +\pard\plain \s6\sb400\sa60\sl280\tx560\tx860 \b\f20 2.17\tab Using the original editor in SAP\par +\pard\plain \s4\qj\sa120\sl280 \f20 This simple editor can insert, delete + and change gel reading sequences by performing one selected operation at a time. It is used during the interactive entry of new readings and interactive joining of contigs. The commands request the position at which the edit is required and the number of +characters to insert, delete or change.\par +\pard\plain \s5\sb400\sa160\sl320\tx560 \b\f20\fs28 3. NOTES\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab +As each reading is entered into a project database it is given a unique number. The first is numbered 1, the second 2 and so on. Their original file names (known as "archives" because they are kept outsid +e the database and never edited) are also copied into the database. During assembly contigs are constantly being changed and reordered so the program identifies them by the numbers or names of the readings they contain. Whenever the program asks users to i +dentify a contig or reading they can type its number or its archive name. If they type its archive name they must precede the name by a slash "/" symbol to denote that it is a name rather than a number. For example if the archive name is fred.gel with numb +er 99, users should type /fred.gel or 99 when asked to identify the contig. Generally, when it asks for the reading to be identified, the program will offer the user a default name, and if the user types only return, that contig will be accessed. When a da +tabase is opened the default contig will be the longest one, but if another is accessed, it will subsequently become the current default. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab An XBAP database is made from five separate files\: the "archive names" file *.ARN, the "relationships" file *.RLN, + the "sequences" file *.SQN, the "tag" file *.TGN, and the "comments" file *.CCN. If the database is called FRED then version 0 of database FRED comprises files FRED.AR0, FRED.RL0, FRED.SQ0, FRED.TG0 and FRED.CC0. The version is the last symbol in the file + names. If the "copy database" option is used it will ask the user to define a new "version". The normal strategy is to use version 0 for all work and to use other versions as backups. Program SAP uses databases formed from only the first three of these f +iles. Normally the program is used to handle DNA sequences but many of the functions also work on protein sequences. The choice of sequence type is made when the database is started.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab The vector sequence should be stored in a simple text file with up to 80 characters of data per line. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab +Almost all readings are assembled automatically in their first pass through the assembly routine. Those that are not can be dealt with in two ways. Either they can be put through assembly again as single named rea +dings (Users should type n when asked "Use file of file names"), with the parameters set to allow the reading in. Or they can be entered through the assembly routine using the "Put all readings in new contigs" mode, and then joined to the contig they overl +ap using the Contig Joining Editor. If it is found that readings are not being assembled in their first pass through the assembler, then it is likely that the contigs require some editing to improve the consensus. Also it may be that poor quality data is b +eing used, possibly by users overinterpretting films or traces. In the long term it can be more efficient to stop reading early and save time on editing. For those using fluorescent sequencing machines the unused data can be incorporated after assembly. +\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Obviously we cannot use a script to operate a program that expects to be controlled by mouse clicks! The program BAP is an xterm version of XBAP which can be used from a script.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab There is a remote possibility of a join being missed by the "Find internal + joins" routine. If a small contig is wholly contained within a larger one, such that its ends are further than ("Probe length" - "Minimum initial match length") from the ends of the larger contig, and the consensus for the small contig lies to the left of + the consensus for large contig, the overlap will not be discovered. (See the search strategy).\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab For those using fluorescent sequencing machines and XBAP the combination of the contig editor and the graphical displays of consensus "quality" will probably + be sufficient for checking and editing contigs as everything can be done at the computer screen. For those using autoradiographs the facility to produce printouts of "display" and "highlight disagreements" options for use while checking films, and the aut +oedit command are most appropriate.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 8.\tab +In general the quality of a reading deteriorates along the length of the gel and so it is also possible to use a length cutoff for the quality calculation. Only the data from the first section of each reading will be included in the calculation. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 9.\tab +There are some limitations on the changes that can be made to the contigs when using the SAP screen editor. Alignments must be maintained during editing. Whole lines of sequence should not be deleted or added unless the order of the gel readings in the +contig is preserved. Each line in the contig display consists of gel reading numbers, their names and 50 character sections of sequence. Insertions are limited in the following way. No line of sequence can be extended rightwa +rds more than 5 characters beyond the end of a full length line (a full length line is 50 characters long). Only one character can be added to the left end of full length lines, but sections of sequence beginning further into a line can be extended leftwar +ds up to an equivalent position. Do not delete any non-sequence lines in the file. Before returning the contig to the database the program checks that the rules have been obeyed. If an error is found the number of the erroneous line in the file is displaye +d and the contig will not be changed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 10.\tab +The following is a justification for using the auto edit function. The general strategy employed when collecting shotgun sequence data is to keep sequencing until the redundancy in the contigs is fairly high, and then to get a printout of a contig, che +ck problems against the films, note corrections on the printout, and make the changes using an interactive editor. In general the consensus is correct except for places where padding characters have been used to accomm +odate a single gel with an extra character, or where the consensus is dash. The important point for the auto editor is that most edits simply make the gel readings conform to the consensus, or remove columns of pads. The auto editor does the following. 1) + calculates a consensus for the contig (or part of a contig) to be edited, and then uses this consensus to direct the editing of the contig in 3 stages 2) stage 1\: + find and correct all places where, if the order of two adjacent characters is swapped, they will both agree with the consensus (given that they did not match the consensus before). These corrections are termed "transpositions" 3) stage 2\: + find and correct all places where there is a definite consensus but the gel reading has a different character. These corrections are termed "changes". 4) stage 3\: + delete all positions in which the consensus is a padding character. These corrections are termed "deletions". All changed characters are shown in uppercase letters so it will be obvious which characters + have been assigned by the program (except for deletions). The number of each type of correction will be displayed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 11.\tab +The "calculate consensus" function, the "display contig" routine, the contig editor and the "show quality" option use the rules outlined here to calculate a consensus from aligned gel readings. The consensus sequence can contain any of 6 possble symbols\: + a,c,g,t,* and -. The last symbols is assigned if none of the others makes up a sufficient proportion of the aligned characters at any pos +ition in the contig. The following calculation is used to decide which symbol to place in the consensus at each position. Each uncertainty code contributes a score to one of a,c,g,t,* and also to the total at each point. Symbols like r and y which don't co +rrespond to a single base type contribute only to the total at each point. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \tab Definite assignments i.e. A,C,G,T,a,c,g,t,b,d,h,v,k,l,m,n,a,c,g,t,* =1 probable assignments i.e. 1,2,3,4 = 0.75 other uncertainty codes including r,y,5,6,7,8,- = 0.1 A cutoff scor +e between 51 and 100% is set by the user. (When the program starts this is set to 75%.). At each position in the contig we calculate the total score for each of the 5 symbols a,c,g,t and * (denote these by Xi, where i=a,c,g,t or *), and also the sum of the +se totals (denote this by S). Then if 100 Xi / S > the cutoff for any i, symbol i is placed in the consensus; otherwise - is assigned. For the "examine quality" algorithm each strand is treated separately but the calculation is the same. \par +12.\tab Databases can + become corrupted if the machine crashes so the programs contain a function "Check database for logical consistency" which checks to see if all the relational data is internally consistent. Some routines automatically perform this check before they start. + Users are advised to make frequent copies of their databases using the "Copy database" option. Note that if BAP is used in "execute with dialogue" mode the "Check logical consistency" function also creates a consensus for the whole database and scans it t +o find any regions which contain 15 dashes in 20 characters. Such a finding would indicate problems with the database.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\pagebb\tx560 13.\tab +We have covered many of the most important or complicated operations peformed by SAP and XBAP, but several others have not been mentioned. These include those for creation of consensus sequence files for processing by other programs, and complementing +contigs, both of which are trivial. There is also a set of routines for fixing corrupted databases.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 14\tab The VAX version of SAP will only a +llow one person to access a sequencing database at a time - producing an "unable to open database" error message if a second person tries. On UNIX machines there is no such check in program SAP so users need to make sure that simultaneous use does not occu +r. Otherwise the data will be corrupted. Program BAP prevents more than one person from using a database at any time. It does so using the following mechanism. When a user requests to open a particular copy (say 0) of a database (say DB) the program checks + for the existence of a file named DB_BUSY0 in the current directory. In normal circumstances, if the file exists, it indicates that somebody else is currently using the database and the program displays the message "Sorry database busy" and does not open +the files. If the file does not exist the program creates it and opens the database. When a user stops using the database (usually by quitting the program) the "busy file" is deleted, hence allowing others to use the database. If the program terminates abn +ormally the busy file will not be deleted and so the database will not be useable until the busy file is explicitly deleted using the rm command. Obviously it is dangerous to delete the file before checking if another user is using the database.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 15\tab +After a run of the assembly routine, reading names can appear in the file of failed reading names for the following reasons. 1. The reading file was not found; 2. the reading file was too short (less than the minimum match length); 3. the reading appear +ed to matc +h somewhere but failed to align sufficiently well (too many padding characters or too high a percentage mismatch); 4. a reading of the same name was already present in the database; 5. the reading was entered but also appeared to match another contig and t +he join was not made. This can occur for two reasons\: a. because the overlap between the two contigs was too large, or b. because after the reading is entered into one contig a new consensus is calculated and compared to the other contig\: + it may then not match as well as it did originally, and the join will not be made.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 16\tab +We have recently devised our own file format (called SCF) for storing traces, sequences and confidence values for data produced by automated sequence readers (Dear and Staden, 1992). For ABI data these typically reduce the storage required to 30% of the + original. Data from the ABI 373A and the Pharmacia A.L.F. can be converted to this form using the program makeSCF. Note that A.L.F. files must first be processed by program alfsplit which s +plits the original data into one file per reading. Sequences can be extracted from SCF files in a form suitable for assembly by use of the program trace2seq. To locate and mark regions of a sequence from an automated sequence reader that are of too low a q +uality to be used for assembly we use the script clip-seqs. This script takes as input a file of reading file names. For each reading it renames the original file "original-filename~" and writes a new file called "original-filename" in which the poor quali +ty regions are marked.\par +\pard\plain \qj \f4\fs16 {\plain \f20 \par +}\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 17\tab The oligo selection engine is the one used in the program OSP. It is described in some detail in\: + Hillier, L., and Green, P. (1991). The parameters controlling the selection of oligos can be changed in the "Oligo Selection Parameters" window. The weigh +ts controlling the scoring of selected oligos can be changed in the "Oligo Selection Weights" window. By default, the oligos are selected from a window that extends 40 bases either side of the cursor. The size and location of this +window relative to the cursor position can be changed in the "Parameters" window. In XBAP oligos are ranked according to their proximity to the cursor position, rather than by their scores. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 18\tab For simplicity, each reading is considered to represent a template. In practise, many readings can be made off the same template. Suitable templates that are identified are those that\: + 1. are in the appropriate sense, 2. have 5' ends that start upstream of the oligo, and 3. are sufficiently close to the o +ligo to be useful. This last criterion relates to the insert size for the subclones used for sequencing and the average reading length. A template is considered useful if a full reading can be made from it, taking into account both of these factors. The d +efault insert size is 1000 bases, and the default average reading length is 400 bases. These values can be changed in the "Parameters" window. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 4.\tab References\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Staden, R. 1982. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. {\i Nucl. Acids Res}. {\b 10 }(15)\:4731-4751.\par +2.\tab Staden, R. 1990. An improved sequence handling package that runs on the Apple Macintosh. Comput. {\i Applic. Biosci}. {\b 4}, 387-393.\par +3.\tab Dear S and Staden,R. 1991. A sequence assembly and editing for efficient management of large projects. {\i Nucl. Acids Res}. {\b 19}, 3907-3911.\par +4.\tab Hillier, L., and Green, P. 1991. "OSP\: an oligonucleotide selection program," PCR Methods and Applications, {\b 1}\:124-128. \par +5.\tab Dear S and Staden, R. 1992. A standard file format for data from DNA sequencing instruments. DNA Sequence, {\b 3}, 107-110.\par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 5. Analysing Sequences to Find Genes\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +2.\tab Methods\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1720 2.1\tab The uneven positional base frequencies method.\par +2.2\tab The positional base preferences method\par +2.3\tab The codon usage method\par +2.4\tab Searching for open reading frames\par +2.5\tab Searching for tRNA genes\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Notes\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 We outline three methods for finding protein genes and one for locating tRNA genes, plus routines for finding open reading frames and displaying the p +ositions of stop codons. All the methods are contained in the program NIP. The correct interpretation of the analyses presented requires a good understanding of the underlying ideas used by the methods. Despite this we concentrate here on the use of the te +chniques and refer the reader to earlier publications (1-5) for more background information. \par +\pard \s4\qj\sa120\sl280 The assumption made by the methods for finding protein genes is that protein coding regions, when analysed in terms of 3 letter nonoverlapping "words", will look +different to noncoding regions analysed in the same way. Suppose we analyse a sequence in one reading frame and count its codons. Then we define the "positional base composition" as the frequency at which each of the four base types occupies each of the th +ree positions in codons. In coding regions the positional base frequencies will be less random than they are in noncoding regions. This is the basis of method 1\: + the "Uneven positional base frequencies method". If this reading frame is coding for a protein + the positional base composition will tend towards a particular bias which is common to the majority of genes. This is the basis of method 2 the "Positional base preferences method". If the sequence has a very biased base composition then in protein genes +this may effect the choice of amino acids, and will effect the use of bases in the third positions of codons. This bias is also utilised by the positional base preferences method. Finally if the reading frame is coding for a protein its use of codons is al +so likely to be nonrandom and this is the basis of method 3, the "Codon usage method".\par +\pard \s4\qj\sa120\sl280 +All the methods perform their analyses over segments of the sequence of size "window", and then move the window on by three bases and repeat the calculation. The "Uneven positional base frequencies" method only produces a single value for each segment and +hence cannot distinguish between frames or strand - it only measures the probability that a region is coding and nothing more. The other two methods produce different va +lues for each of the three potential reading frames and hence can help to decide which is coding. Their results are plotted in three separate boxes arranged one above the other. For these we also indicate which of the three reading frames is the highest sc +oring at each position along the sequence. This is done by plotting a single dot at the mid-height of the box that contains the highest score, so that if one frame is the highest scoring for many consecutive positions, the dots will produce a solid line at + the mid-height of its box. We also mark the positions of stop codons. These are represented by short vertical lines and are positioned so that they bisect the mid-height of each box. Start codons are marked at the base of the box for each reading frame. +\par +\pard \s4\qj\sa120\sl280 The search for tRNA genes involves looking for segments that could fold into the cloverleaf structure and which have the expected conserved bases in the appropriate positions.\par +\pard \s4\qj\sa120\sl280 Notice that we have not mentioned searches for relevent "signals" like promoters +or splice junctions which are also useful for finding genes. These searches are described in the chapter on searching for motifs. In the current chapter the only "signal" we include is the stop codon. However as all results are presented graphically it is +easy for users to overlay the displays of signal searches with those presented here and so effectively combine them.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 2.\tab Methods\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.1\tab The uneven positional base frequencies method.\par +\pard\plain \s4\qj\sa120\sl280 \f20 This method produces a single value for each segment of the sequence, and wou +ld give the same result if applied to each reading frame or to the complementary strand. The results are plotted in a box that is cut by a horizontal line. This line is labelled 76% and we expect 76% of noncoding sequences to score below this line and 76% +of coding sequence to score above it. Of the methods described this one makes the fewest assumptions and so is a good unbiased indicator of the probability that a sequence is coding.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Uneven positional base frequencies".\par +2.\tab Define "Odd window length". \par +3.\tab Define "Plot interval".\par +\pard\plain \s4\qj\sa120\sl280 \f20 The plot will appear as in figure 5.1. In the example shown the 5' end of the sequence codes for several proteins and the 3' end codes for ribosomal RNAs.\par +\pard\plain \li100\sb300\sl160\keepn \f4\fs16 {\plain \fs16 {\pict\macpict\picw436\pich41 +1103ffffffff002801b31101a00082a0008c01000affffffff002801b3070000000022000100010000a000a0a100a400020de801000a00000000000000000700010001220027000100da23000021000101b22300002300262300002100270001230000a000a301000affffffff002801b32300da21000101b2230026210027 +0001a000a12000170001001701b2220025000100df2300032300062301002300fb2300fd2300022300fe2302032300ff2300002300fe2300fd2301002300032300002300fd2302022300042300002300052300002301fd2300002300032300002300012302fd2300fd2300002300fd2300ff2301fe23000023000023000223 +00062302012300fc2300032300012300002301052300062300fa2300f82302ff2300fb2300002300002300002301002300002300002300002300002302002300002300032300052300012301092300ff2300042300022300002302042300fa2300fc2300fe2300022301002300fd2300002300002302032300002300fe2300 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+fe2300022300002300fe2302002300fd2300002300f92300fd2301092300002300092300012300022302002300032300002300032300002301002300f52300fa2300032302fd2300002300fc23000923000b2301f92300002300fd2300fa2300fd2302062300052300012300ff2300032300fb2301072300042300002300ff +2300fe2302022300012300002300002300002302002300002300fc2300fea0008da00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 5.1.\tab Example output from the uneven positional base frequencies method. The 5' end codes for proteins and the 3' end contains ribosomal RNA genes.\par +\pard\plain \s6\sb360\sa60\sl280\tx560\tx860 \b\f20 2.2\tab The positional base preferences method\par +\pard\plain \s4\qj\sa120\sl260 \f20 As a result of the genetic code and the relative frequencies with which amino acids are used in proteins, DNA sequences codi +ng for proteins have a particular bias in their positional base frequencies. This method scans DNA sequences and measures the closeness of each reading frame to this bias in their positional base frequencies. The closeness to the expected bias is expressed + as a \: +"score". By default the program will use a "global" set of expected values for the positional base frequencies which are derived from average amino acid compositions in known proteins. Alternatively users may create their own set of expected values +by analysing known genes from the same genome. In addition users can combine the "global" values for the first two positions in codons with third position values derived from other genes of the same genome.\par +\pard \s4\qj\sa80\sl260 +In order to use a nonglobal standard, a codon table in the format described in the chapter on statistical analysis of nucleic acid sequences, can be created using the method "Creating a codon usage file". Alternatively a section of the sequence being analy +sed can be scanned to produce an internal standard. The method is particularly useful for selecting which reading frame is coding.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.2.1\tab Using the global standard\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Positional base preferences method".\par +2.\tab Select "Standard source" as "Global".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Define "Window length". The default length of 67 should be used for most cases. Shorter windows give noisier plots and the longer the window the more chance there is of missing a short exon.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Plot interval".\par +\pard\plain \s4\qj\sa120\sl260 \f20 The plot will appear as in figure 5.2. This shows a 10,000 base section of sequence tha +t codes for several proteins in each of the three reading frames. See the introduction for an explanation of the plotting scheme used.\par +\pard\plain \s8\qj\fi-1140\li1140\sb300\sa120\sl240\keepn\tx1140 \f21\fs20 {{\pict\macpict\picw447\pich225 +0d7effffffff00e001be1101a0008201000affffffff00e001be090000000000000000310000000000df01bd98002400000000008d012000000000008d011f0000000000df01bd000102dd0006007fdfff00fc140040ed000e01f000e1ffffebffff87ff83d40004140040ed000e0110009200002a00008800425c00041400 +40ed000e2908009200002c00007800442a0004140040ed000e5a08008c0000140000400044220004170040f3000008fc00068608010c000010fd000324220004170078f3000008fc0002860501fe000010fd000328020004130040f3000008fc0002800701f9000328020004130040f3000014fc0002800101f90003300100 +04150040f30008140000100000800087f9000310010004150040f300081400001800010000a4f9000310010004130040f300081400002400010000e4f700018004130040f30008240000240001000018f700018004130040f30008220000240001000018f700018004130040f30008220000220002000018f7000180041300 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+f3000120041b09400080000001000054a4fe000664248100200201f400022020041f0040fc0003c0004364fe000c60288200204201000004000002fa00023040041e0040fc0002a00043fd000c4018820020a40100000c000102fa00023040041e0040fc0002a00040fd000c4010620020a40100000a000102fa0002314004 +1c0040fc0002200080fb000a6200132801000012000142fa00023140041f0078fc0002100080fb000a24001318010000121002cdfd00050800004a80041f0040fc0002100080fb000a14001b10010000111802adfd00050800004a80041f0040fc0002080080fb000a14001c0000880021e802b5fd0005140000ca80041f00 +40fc00010801fa00141400040000740020280c35803400001400014400041f0040fc00010c01fa00141800040000440040240c04803600001400010400042523400000aa0a020ec280020c801021001c809050009204c405501c846aee0573625284900c2523400000aa0a020ffe80020c801021001c8090500091ffc407f0 +1cffebfffff3fffe84900c06007fdfff00fc02dd00a00083ff}}\par +\pard \s8\qj\fi-1140\li1140\sa120\sl240\tx1140 Figure 5.2\tab Example output from the positional base preferences method. Most of the sequence is coding for proteins.\par +\pard\plain \s9\fi-560\li860\sb400\sa60\sl280\tx1140 \b\f20 2.2.2\tab Using a nonglobal standard\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Make an appropriate codon usage file as described in the chapter on statistical analysis of nucleotide sequences.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Select "Positional base preferences method".\par +3.\tab Select "Standard source" as "Codon usage table".\par +4.\tab Define "File name of standard". The file will be read and displayed on the screen.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab +Select "Normalisation" as "Combine with global standard". This alternative means we will use the values for the first two positions of codons combined with the third position values from our codon table. Otherwise ("Use observed frequencies") will use a +ll three positions from our codon table. The positional base frequencies to be used will be displayed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Accept "Use 1.0 for positional weights". The alternative allows users to +give greater or lesser emphasis to any of the three positions by defining weights for each. The program displays the "Expected scores per codon in each frame".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Define "Window length". Windows shorter than the default of 67 may be useful if the bias is sufficiently strong. Look at the "Expected scores in each frame" to help decide.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 8.\tab Define "Plot interval".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 9.\tab Accept "Plot relative scores". This means that for each frame we plot its score divided by the sum of the scores for all three frames. It produces + smoother plots than the alternative "Plot absolute scores" which simply plots the scores for each frame. The minimum and maximum expected scores for the given standard and window length are displayed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 10.\tab Accept "Leave scaling values unchanged". The expected scores just displayed will be used to scale the plots. If required the user can change the scaling values at this point.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 The plot will now appear as in figure 5.2. Typical dialogue is shown in figure 5.3.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.3\tab The codon usage method\par +\pard\plain \s4\qj\sa120\sl280 \f20 The codon usage meth +od scans along a sequence and measures the closeness of each reading frames codon composition to an expected set of codons. Of the methods described it is the most sensitive, but consequently has to make the strongest assumption, namely that we know the ap +proximate codon usage for the genes being searched for. The codon usage will depend on the codon preferences and the amino acid composition of the protein product. For this reason the program contains three methods of "normalisation". The table of codon us +age may be used as read "Observed frequencies"; the table may be transformed to reflect an average amino acid composition "Normalise to average amino acid composition"; the table may be transformed to have no amino acid bias "Normalise to no amino acid bia +s". The table can be read from a file produced by "Creating a codon usage file" as described in the chapter on statistical analysis of nucleic acid sequences, or an "internal standard" can be used by the user defining a region of the current sequence. In t +he latter case the program will calculate the codon usage for the defined region.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Codon usage method".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Reject "Define internal standard". If an internal standard is used the program will ask for the end points of the segments over which to calculate the codon usage.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Define "File name of standard". The file will be read and displayed on the screen.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Select "Normalisation" as "Average amino acid composition". The program will display the expected values for each reading frame for the window lengths 21, 31 and 41 codons. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Select "Window length".\par +6.\tab Select "Plot interval".\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The plot will appear as in figure 5.4. This shows a 10,000 base section of sequence that codes for several proteins in each of the three reading frames. See the introduction for an explanation of the plotting scheme used.\par +\pard\plain \li1840\ri1980\sl220\pagebb\box\brsp100\brdrth \f4\fs16 Positional base preferences method to find protein genes\par +\pard \li1840\ri1980\sl220\box\brsp100\brdrth Select standard source\par +X 1 Use global standard\par + 2 Use internal standard\par + 3 Use codon usage table\par +? Selection (1-3) (1) =3\par +? File name of standard=atpase.cods\par + ===========================================\par + F TTT 21. S TCT 33. Y TAT 15. C TGT 5.\par + F TTC 55. S TCC 40. Y TAC 40. C TGC 4.\par + L TTA 8. S TCA 7. * TAA 8. * TGA 0.\par + L TTG 19. S TCG 12. * TAG 1. W TGG 17.\par + ===========================================\par + L CTT 22. P CCT 17. H CAT 6. R CGT 73.\par + L CTC 21. P CCC 4. H CAC 30. R CGC 23.\par + L CTA 1. P CCA 10. Q CAA 19. R CGA 5.\par + L CTG 168. P CCG 48. Q CAG 80. R CGG 3.\par + ===========================================\par + I ATT 47. T ACT 14. N AAT 17. S AGT 8.\par + I ATC 98. T ACC 54. N AAC 52. S AGC 26.\par + I ATA 6. T ACA 7. K AAA 85. R AGA 0.\par + M ATG 75. T ACG 13. K AAG 28. R AGG 0.\par + ===========================================\par + V GTT 67. A GCT 56. D GAT 41. G GGT 90.\par + V GTC 29. A GCC 53. D GAC 66. G GGC 66.\par + V GTA 49. A GCA 59. E GAA 101. G GGA 5.\par + V GTG 57. A GCG 64. E GAG 41. G GGG 8.\par + ===========================================\par +Select normalisation\par +X 1 Use observed frequencies\par + 2 Combine with global standard\par +? Selection (1-2) (1) =2\par + T C A G Range\par + 1 0.177 0.211 0.277 0.336 0.159\par + 2 0.271 0.238 0.310 0.182 0.128\par + 3 0.242 0.301 0.168 0.289 0.132\par +? Use 1.0 for positional weights (y/n) (y) =\par + Expected scores per codon in each frame\par + 0.785 0.736 0.736\par +? odd span length (31-101) (67) =\par +? plot interval (1-11) (5) =\par +? Plot relative scores (y/n) (y) =\par +\par + Minimum maximum range\par + 0.3219 0.3519 0.0214\par +\pard \li1840\ri1980\sl220\keepn\box\brsp100\brdrth ? Leave scaling values unchanged (y/n) (y) =\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 5.3\tab +Typical dialogue from the "Positional base preferences method" using a nonglobal standard in the form of a codon table to specify the values for the third positions in codons.\par +\pard\plain \s6\sb400\sa60\sl280\tx560\tx860 \b\f20 2.4\tab Searching for open reading frames\par +\pard\plain \s4\qj\sa120\sl280 \f20 This routine finds all open reading frames of some minimum length and writes its results in the form of an EMBL feature table. \par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Find open reading frames".\par +\pard\plain \sl220\keepn \f4\fs16 {{\pict\macpict\picw442\pich218 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\s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 5.4\tab Example output from the codon usage method. Most of the sequence is coding for proteins.\par +\pard\plain \s7\qj\fi-560\li560\sb400\sa120\sl280\tx560 \f20 2.\tab Define "Minimum open frame in amino acids".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Select "Strands". The alternatives are\: + strand only, - strand only, or both strands. Typical output is shown in figure 5.5.\par +\pard\plain \li2120\ri2240\sb400\sl220\box\brsp100\brdrth \f4\fs16 FT CDS 525..965 \par +\pard \li2120\ri2240\sl220\box\brsp100\brdrth FT CDS 956..1789 \par +FT CDS 2128..2607 \par +FT CDS 2604..3155 \par +FT CDS 3159..4709 \par +FT CDS 4733..5623 \par +FT CDS 5539..7032 \par +FT CDS 7044..7454 \par +FT CDS 7797..8134 \par +FT CDS complement(2227..2634)\par +FT CDS complement(2250..3023)\par +FT CDS complement(3027..3899)\par +FT CDS complement(3903..4760)\par +FT CDS complement(4327..4626)\par +FT CDS complement(4646..5332)\par +FT CDS complement(5345..5647)\par +FT CDS complement(5635..6012)\par +FT CDS complement(6016..6441)\par +FT CDS complement(6445..7083)\par +FT CDS complement(7035..7445)\par +\pard \qj\li2120\ri2240\sl220\keepn\box\brsp100\brdrth FT CDS complement(7406..7777)\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 5.5\tab Typical output from "Find open reading frames"\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.5\tab Searching for tRNA genes\par +\pard\plain \s4\qj\sa120\sl280 \f20 tRNA genes have two classes of feature that can be used to locate them in genomic sequences\: + their ability to fold into the cloverleaf secondary structure, and the presence of specific "conserved" bases at particular positions relative to this structure. The level of congruence with the canonical structure is quite variable\: + some tRNA genes contain intervening sequences and others, particular those from organelles, have few of the conserved bases. The program searches for potential cloverleaf forming str +uctures and optionally the presence of conserved bases. The user can define the range of loop sizes, the minimum numbers of potential base pairs, a range of intron sizes, and which, if any, of the conserved bases should be present. The results are presente +d either textually or graphically. \par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "tRNA search".\par +2.\tab Define "Maximum tRNA length".\par +3.\tab Define "Aminoacyl stem score". See note 8.\par +4.\tab Define "Tu stem score".\par +5.\tab Define "Anticodon stem score".\par +6.\tab Define "D stem score".\par +7.\tab Define "Minimum base pairing total".\par +8.\tab Define "Minimum intron length".\par +9.\tab Define "Maxmimum intron length".\par +10.\tab Define "Minimum length for TU loop".\par +11.\tab Define "Maximum length for TU loop".\par +12.\tab Accept "Skip search for conserved bases". See notes section.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 13.\tab Reject "Plot results". +This gives listed output in which the potential cloverleafs are displayed. The alternative plotted output simply draws a vertical line to represent the score for the potential gene, at the position it has been found. Typical dialogue and the beginning of s +ome listed output is shown in figure 5.6.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 3.\tab Notes\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab +In general, for finding protein genes, we recommend the use of all the methods. The "Uneven positonal base frequencies" method can show which regions are likely to be coding but not which strand or fram +e. The "Positional base preferences" method can show the correct frame and also help to find which regions are coding. The "Codon usage" method has the greatest resolution, having been used successfully with windows of 11 codons, and can help find small ex +ons and to pinpoint exon/intron boundaries.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab +When the "Uneven positional base frequencies" calculation was applied to all the sequences in the 1984 version of the EMBL library 14% of noncoding segments failed to reach the value represented by the base of + the box, whereas all coding segments did. The top value of the box was not reached by any noncoding segments but was exceeded by 16% of coding sequences. 76% of noncoding segments failed to reach the line labelled 76% but 76% of coding segments fell above + it. We would not expect this result change significantly if it were to be recalculated on the current libraries.\par +3.\tab When the "Positional base preferences" method, using "global" values, was applied to all the {\i E. coli} genes in the 1984 version of the EMBL library it chose the correct reading frame for 91% of coding segments. {\i E. coli} + sequences were used for technical rather than scientific reasons and we have no reason to believe that other organisms should give significantly different results. This result used only the values for the first two positions in codons and so for genes wit +h a strongly biased base composition we would expect even better discrimination.\par +\pard\plain \li1180\ri1440\sb100\sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth \f4\fs16 tRNA search\par +\pard \li1180\ri1440\sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth ? Maximum trna length (70-130) (92) =\par +? Aminoacyl stem score (0-14) (11) =\par +? Tu stem score (0-10) (8) =\par +? Anticodon stem score (0-10) (8) =\par +? D stem score (0-8) (3) =\par +? Minimum base pairing total (30-44) (30) =\par +? Minimum intron length (0-30) (0) =\par +? Maximum intron length (0-30) (0) =\par +? Minimum length for TU loop (4-12) (6) =\par +? Maximum length for TU loop (6-12) (9) =\par +? Skip search for conserved bases (y/n) (y) =n\par +Give a score for each base, then a minimum total at the end\par +? Base 8, T is 100% conserved. Score (0-100) (0) =\par +? Base 10, G is 95% conserved. Score (0-100) (0) =\par +? Base 11, Y is 96% conserved. Score (0-100) (0) =\par +? Base 14, A is 100% conserved. Score (0-100) (0) =\par +? Base 15, R is 100% conserved. Score (0-100) (0) =\par +? Base 21, A is 97% conserved. Score (0-100) (0) =\par +? Base 32, Y is 100% conserved. Score (0-100) (0) =\par +? Base 33, T is 98% conserved. Score (0-100) (0) =\par +? Base 37, A is 91% conserved. Score (0-100) (0) =\par +? Base 48, Y is 100% conserved. Score (0-100) (0) =\par +? Base 53, G is 100% conserved. Score (0-100) (0) =\par +? Base 54, T is 95% conserved. Score (0-100) (0) =\par +? Base 55, T is 97% conserved. Score (0-100) (0) =\par +? Base 56, C is 100% conserved. Score (0-100) (0) =\par +? Base 57, R is 100% conserved. Score (0-100) (0) =\par +? Base 58, A is 100% conserved. Score (0-100) (0) =\par +? Base 60, Y is 92% conserved. Score (0-100) (0) =\par +? Base 61, C is 100% conserved. Score (0-100) (0) =\par +? Minimum total conserved base score (0-0) (0) =\par +? Plot results (y/n) (y) =n\par + 264\par + t\par + t-a\par + c-g\par + a-t\par + t+g\par +\pard \li1180\ri1440\sl220\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth a-t\par + a a\par + a-t gta\par + c aacgc\par + a t !!!! c\par + cgt gtgcg a\par + !!! t cga\par + a gca c\par + g t g\par + c aa t\par + a-t a\par + t-a t a\par + t-a\par + t-a\par + g t\par + c g\par +\pard \li1180\ri1440\sl220\keepn\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth caa\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 5.6\tab Typical dialogue and textual output from "Find tRNA genes".\par +\pard\plain \s7\qj\fi-560\li560\sa80\sl280\tx560 \f20 4.\tab If the codon table used by the "Codon usage" me +thod is normalised to have average amino acid composition it retains its codon preference bias for each amino acid type but now the amino acid composition is the average of all proteins. In general this is optimal\: + we have the expected codon preference bia +s plus an expected amino acid bias. If we normalise to no amino acid bias we are safeguarding ourselves against missing a protein of anomalous composition but at the expense of not employing all of the useful information for distinguishing coding from nonc +oding. \par +\pard \s7\qj\fi-560\li560\sa80\sl280\tx560 5.\tab +The program also contains a graphical version of Ficketts method (6), except here we use a window to analyse each segment of the sequence rather than giving a single value for each open reading frame. The tables used are those from the original publicat +ion.\par +\pard \s7\qj\fi-560\li560\sa80\sl280\tx560 6.\tab If the results from the "Find open reading frames" option are directed to disk (See the introductory chapter), the file can be used by the routines that use feature tables as input.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab The program also contains several routines for plotting the positions of stop and start codons for either strand of the sequence. One form of the output is included in figures 5.2 and 5.4.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 8.\tab The tRNA gene search using a simple scoring system for base pairing\: + A-T and G-C base pairs each score 2 and G-T scores 1. The use of a "Minimum base pairing total" allows low cutoffs to be set for each individual stem, but that overall some reasonable +level of stability is possible. In this way a low score for one stem can be compensated by a high score in another.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 10.\tab Th +e cloverleaf is composed of four base-paired stems and four loops. Three of the stems are of fixed length but the fourth, the dhu stem which usually has four base pairs, sometimes has only three. All of the loops can vary in size. The following relationshi +ps between the stems in the cloverleaf are assumed in the program\: + (a) there are no bases between one end of the aminoacyl stem and the adjoining tuc stem; (b) there are two bases between the aminoacyl stem and the dhu stem; (c) there is one base between t +he dhu stem and the anticodon stem; (d) there are at least three bases between the anticodon stem and the tuc stem.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 4.\tab References\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Staden, R. and McLachlan, A.D. 1982. Codon preference and its use in identifying protein coding regions in long DNA sequences. {\i Nucl. Acids Res.} {\b 10}\:151-156.\par +2.\tab Staden, R. 1984. Measurements of the effects that coding for a protein has on a DNA sequence and their use for finding genes. {\i Nucl. Acids Res}. {\b 12}\:551-567.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Staden, R. 1985. Computer methods to locate genes and signals in nucleic acid sequences. (in) {\i Genetic Engineering, Principle and Methods}, Setlow J.K., Hollaender A., (eds.), {\b 7}\: +67-114, (Plenum Press, New York).\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Staden, R. 1990. Finding Protein Coding Regions in Genomic Sequences. (in) {\i Methods in Enzymology} R.F. Doolittle (ed.), {\b 183}\:163-180 (Academic Press, New York).\par +5.\tab Staden, R. 1980. A computer program to search for tRNA genes. {\i Nucl. Acids Res}. {\b 8}\:817-825.\par +6.\tab Fickett, J.W. 1982. Recognition of protein coding regions in DNA sequences. {\i Nucl. Acids Res}. {\b 10}\:5303-5318.\par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 6. Searching for Motifs in Nucleic Acid Sequences\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +2.\tab Methods\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.1\tab Searching for percentage matches to consensus sequences\par +2.2\tab Searching for consensus sequences using a score matrix\par +2.3\tab Using weight matrices for searching nucleotide sequences\par +2.4\tab Using "hardwired" motif searches.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Notes\par +4.\tab References\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 The program NIP contains several ways of defining and searching for motifs (1-4), and also contains a number of "hardwired" motifs that are already +defined and can be selected as separate searches. We describe searches for percentage matches to consensus sequences, the use of score matrices and the creation and use of nucleotide and dinucleotide weight matrices (see note 7). In addition we give detail +s of the "hardwired" motifs available from the program. In another chapter we have covered searches for exact matches to consensus sequences by describing how to find restriction enzyme recognition sequences. When searching for exact matches, percentage ma +tches or using a score matrix the search string or consensus sequence may include IUB redundancy codes. All of the searches produce both listed and graphical output. The listed output displays the matching sequence and its position and the graphical output + draws a box to represent the length of the sequence, and plots vertical lines within the box at the positions of matches. The heights of the lines are proportional to the match score (see figure 6.1).\par +\pard\plain \sb200\sl220\keepn \f4\fs16 {{\pict\macpict\picw447\pich44 +032fffffffff002b01be1101a0008201000affffffff002b01be0900000000000000003100000000002a01bd98002400000000001d012000000000001d011f00000000002a01bd000102dd0006007fdfff00fc060040df000004060040df000004060040df0000041002400088f7000020f1000001fd0000041002400088f7 +000020f1000001fd0000041002400088f7000020f1000001fd00000421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe0005014200 +05c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020 +012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc0003021004 +60fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482 +b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00421044482b81210fc000302100460fc00078080000020012008fe000501 +420005c00421044482b81210fc000302100460fc00078080000020012008fe000501420005c00406007fdfff00fc02dd00a00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 6.1\tab Typical graphical output from a motif sea +rch. It shows a rectangular box in which each match is identified by a vertical line whose height gives the match score and whose x coordinate indicates the position in the sequence.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 2.\tab Methods\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.1\tab Searching for percentage matches to consensus sequences\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Find percentage matches".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Accept "Type in strings". The alternative allows the string to be extracted from a named file.\par +3.\tab Reject "Keep picture". This will cause the graphics window to be cleared. The alternative leaves it unchanged.\par +4.\tab Define "String". Type in the search string. When the program cycles round to this point again the previous string will be offered as a default.\par +5.\tab Accept "This sense". The alternative directs the program to search for the complement of the string.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Define "Percent match". The search is performed, the results are presented graphically (see figure 6.1), the number of matches displayed, and the scores and positions of the top 10 matches displayed. +\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Define the number of matches to "Display". For the number of mat +ches chosen the program will display the search string and matching sequence written one above the other with matching characters indicated by asterisk symbols. The program now cycles round to step 3. See figure 6.2.\par +\pard\plain \li220\ri280\sb400\sl220\box\brsp100\brdrth \f4\fs16 Find percentage matches\par +\pard \li220\ri280\sl220\box\brsp100\brdrth ? Type in string (y/n) (y) =\par + ? Keep picture (y/n) (y) =\par + ? String=AAAATTTT\par +STRING=AAAATTTT\par +? This sense (y/n) (y) =\par + ? Percent match (1.00-100.00) (70.00) =\par +\par +Total scoring positions above 70.000 percent = 41\par +Scores 7 7 7 7 6 6 6 6 6 6\par +Positions 428 534 2994 7026 130 191 192 372 427 429\par +? Display (0-41) (0) =4\par +\par + 428\par + aaaatatt\par + ***** **\par + AAAATTTT\par + 1\par +\par + 534\par + aaagtttt\par + *** ****\par + AAAATTTT\par + 1\par + 2994\par + aaaatttc\par + *******\par + AAAATTTT\par + 1\par +\par + 7026\par + aaaacttt\par + **** ***\par + AAAATTTT\par +\pard \li220\ri280\sl220\keepn\box\brsp100\brdrth 1\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 6.2\tab Worked example for the percentage match search\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 \page 2.2\tab Searching for consensus sequences using a score matrix\par +\pard\plain \s4\qj\sa120\sl280 \f20 +A score matrix gives a score for the alignment of each possible pair of sequence symbols. The matrix used by this program includes all the IUB redundancy codes and gives scores that represent the level of redundancy. The matrix is shown in figure 6.3. +\par +\pard\plain \s7\qj\fi-560\li560\sb200\sa120\sl280\tx560 \f20 1.\tab Select "Find matches using a score matrix".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Accept "Type in strings". The alternative allows the string to be extracted from a named file.\par +3.\tab Reject "Keep picture". This will cause the graphics window to be cleared. The alternative leaves it unchanged.\par +4.\tab Define "String". Type in the search string. When the program cycles round to this point again the previous string will be offered as a default.\par +5.\tab Accept "This sense". The alternative directs the program to search for the complement of the string. The program displays the maximum possible score for the string.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Define "Score". The search is performed, the results are presented graphically (see figure 6.1), the number of matches displayed, and the scores and positions of the top 10 matches displayed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab +Define the number of matches to "Display". For the number of matches chosen the program will display the search string and matching sequence written one above the other with matching characters indicated by asterisk symbols. The program now cycles round + to step 3. The dialogue shown in figure 6.2 is almost exactly the same as that for "Searching for consensus sequences using a score matrix".\par +\pard\plain \li1580\ri1560\sb300\sl220\box\brsp100\brdrth \f4\fs16 T C A G - R Y W S M K H B V D N ?\par +\pard \li1580\ri1560\sl220\box\brsp100\brdrth T 36 0 0 0 9 0 18 18 0 0 18 12 12 0 12 9 0\par +C 0 36 0 0 9 0 18 0 18 18 0 12 12 12 0 9 0\par +A 0 0 36 0 9 18 0 18 0 18 0 12 0 12 12 9 0\par +G 0 0 0 36 9 18 0 0 18 0 18 0 12 12 12 9 0\par +- 9 9 9 9 36 18 18 18 18 18 18 27 27 27 27 36 0\par +R 0 0 18 18 18 36 0 9 9 9 9 6 6 12 12 18 0\par +Y 18 18 0 0 18 0 36 9 9 9 9 12 12 6 6 18 0\par +W 18 0 18 0 18 9 9 36 0 9 9 12 6 6 12 18 0\par +S 0 18 0 18 18 9 9 0 36 9 9 6 12 12 6 18 0\par +M 0 18 18 0 18 9 9 9 9 36 0 12 6 12 6 18 0\par +K 18 0 0 18 18 9 9 9 9 0 36 6 12 6 12 18 0\par +H 12 12 12 0 27 6 12 12 6 12 6 36 8 8 8 27 0\par +B 12 12 0 12 27 6 12 6 12 6 12 8 36 8 8 27 0\par +V 0 12 12 12 27 12 6 6 12 12 6 8 8 36 8 27 0\par +D 12 0 12 12 27 12 6 12 6 6 12 8 8 8 36 27 0\par +N 9 9 9 9 36 18 18 18 18 18 18 27 27 27 27 36 0\par +\pard \li1580\ri1560\sl220\keepn\box\brsp100\brdrth ? 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 6.3\tab The DNA score matrix using IUB symbols\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.3\tab Using weight matrices for searching nucleotide sequences\par +\pard\plain \s4\qj\sa120\sl280 \f20 A we +ight matrix is the most sensitive way of defining a motif. It is a table of values that gives scores for each base type in each position along a motif. For a motif of length 8 bases the weight matrix would be a table 8 positions long and 4 deep. The simple +st way of choosing the values for the table is to take an alignment of all known examples of the motif and to count the frequency of occurrence of each base type at each position. These frequencies can be used as the table of weights. When the table is use +d to search a new sequence the program calculates a score for each position along the sequence by adding or multiplying (see note 6) the relevant values in the table. All positions that exceed some cutoff score are reported as matching the original set of +motifs.\par +\pard \s4\qj\sa120\sl280 +How can we select a suitable cutoff score? The simplest way is to apply the weight matrix to all the known occurrences of the motif - i.e. the set of sequence segments used to create the table - and to see what scores they achieve. The cutoff can b +e selected accordingly. For convenience the weight matrix is stored as a file along with its cutoff score, a title that is displayed when the file is read, and a few other values need by the program. A routine for creating weight matrix files from sets of +aligned sequences is included in the program. When a search using the weight matrix is performed the program will either list the matching sequence segments or plot their positions as for the other motif search methods.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.3.1\tab Creating a weight matrix file from a set of aligned sequences\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Motif search using weight matrix".\par +2.\tab Select "Make weight matrix".\par +\pard \s7\qj\fi-560\li560\sa120\sl260\tx560 3.\tab +Define "Name of aligned sequences file". We assume the file of aligned sequences has already been created (See note 3). The program reads and displays the contents of the file numbering each sequence as it goes. Then it displays the length of the longes +t sequence.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Accept "Sum logs of weights". The alternative is to sum the weights when calculating scores (see note 4). \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Accept "Use all motif + positions". The alternative allows the user to define a "mask" which identifies positions within the motif that should be ignored when the matrix is created (see note 5). The program now calculates the weights and applies them in turn to each of the seque +nces in the file. The number and score for each sequence is displayed, followed by the top, bottom and mean scores and the standard deviation. In addition the mean plus and minus 3 standard deviations is displayed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Define "Cutoff score". The default is the mean minus 3 standard deviations, but users may, for example, decide to use the lowest score obtained by the sequences in the file.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Define "Top score for scaling plots". This parameter is used by the graphics output routine when scaling the plots. Its value will influence the height of lines plotted to represent matches.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 8.\tab Define "Position to identify". When a search is performed it is not always appropriate to report the position of a match relative to the leftmost base in the motif. For example wh +en performing a splice junction search we may want to know the position of the G in the conserved GT, rather than the position of the first base in the matrix. The "Position to identify" allows the user to define which base is marked. The bases in the tabl +e are number 1,2,3 and so on.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 9.\tab Define a "Title". This is a title that will be displayed when the matrix file is read prior to performing a search. It is limited to 60 characters.\par +10.\tab Define "Name for new weight matrix file". Give a name for the weight matrix file. Typical dialogue is shown in figure 6.4.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 \page 2.3.2\tab Searching using a weight matrix\par +\pard\plain \s4\qj\sa120\sl280 \f20 Once a weight matrix has been stored in a file it can be used to search any sequence. Results can be displayed graphically or the matching sequence segments can be listed out with their scores.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Motif search using weight matrix".\par +2.\tab Select "Use weight matrix".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Define "Motif weight matrix file". The name of the file containing the weight matrix. The program reads the file and displays its title.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define +"Cutoff score". The default will be the value set when the weight matrix file was created. If the score is negative the program will calculate sums of logs of frequencies, otherwise it will add frequencies.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Accept "Plot results". Alternatively they will be listed.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The results will appear as in figure 6.5\par +\pard\plain \li1440\ri1500\sb200\sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth \f4\fs16 Motif search using weight matrix\par +\pard \li1440\ri1500\sl220\brdrl\brsp100\brdrth \brdrr\brsp100\brdrth Select operation\par +X 1 Use weight matrix\par + 2 Make weight matrix\par + 3 Rescale weight matrix\par +? Selection (1-3) (1) =2\par +? Name of aligned sequences file=heatshock.seq\par + 1 ATAAAGAATATTCTAGAA\par + 2 CTCGAGAAATTTCTCTGG 144\par + 3 TTCTCGTTGCTTCGAGAG 36\par + 4 GCCTCGAATGTTCGCGAA 15\par + 5 GACTGGAATGTTCTGACC 45 DROSOPHILA HSP68\par + 6 ATCTCGAATTTTCCCCTC 12\par + 7 ATCCAGAAGCCTCYAGAA 35 DROSOPHILA HSP83\par + 8 CTCTAGAAGTTTCTAGAG 25\par + 9 TTCTAGAGACTTCCAGTT 15\par + 10 CCCCAGAAACTTCCACGG 147 DROSOPHILA HSP22\par + 11 GCGAAGAAAATTCGAGAG 46\par + 12 TGCCGGTATTTTCTAGAT 26\par + 13 CCCGAGAAGTTTCGTGTC 97 DROSOPHILA HSP23\par + 14 TTCCGGACTCTTCTAGAA 13 DROSOPHILA HSP26\par + 15 CTCGAGAAAGCTCGCGAA 204 XENOPUS HSP70\par + 16 CTCGCGAATCTTCCGCGA 194\par + 17 CTCGCGAAAGTTCTTCGG 139\par + 18 CTCGGGAAACTTCGGGTC 72\par + 19 TGCCAGAAGTTGCTAGCA 124 XENOPUS HSP30\par + 20 CTCGGGAACGTCCCAGAA 14\par + 21 ATCCCGAAACTTCTAGTT 129 SOYBEAN HSP17\par + 22 GTCCAGAATGTTTCTGAA 98\par + 23 TTTCAGAAAATTCTAGTT 78\par + 24 CCCAAGGACTTTCTCGAA 28\par + 25 TTTTAGAATGTTCTAGAA 179 DICTYOSTELIUM DIRS-1\par + 26 TTCTAGAACATTCGAAGA 169\par +Length of motif 18\par +? Sum logs of weights (y/n) (y) =\par + ? Use all motif positions (y/n) (y) =\par + Applying matrix to input sequences\par + 1 -15.609 ATAAAGAATATTCTAGAA\par + 2 -15.965 CTCGAGAAATTTCTCTGG\par + 3 -18.186 TTCTCGTTGCTTCGAGAG\par + 4 -15.331 GCCTCGAATGTTCGCGAA\par + 5 -20.897 GACTGGAATGTTCTGACC\par + 6 -17.347 ATCTCGAATTTTCCCCTC\par + 7 -16.271 ATCCAGAAGCCTCYAGAA\par + 8 -12.227 CTCTAGAAGTTTCTAGAG\par + 9 -15.933 TTCTAGAGACTTCCAGTT\par + 10 -15.604 CCCCAGAAACTTCCACGG\par + 11 -17.866 GCGAAGAAAATTCGAGAG\par + 12 -17.159 TGCCGGTATTTTCTAGAT\par + 13 -16.399 CCCGAGAAGTTTCGTGTC\par + 14 -14.646 TTCCGGACTCTTCTAGAA\par + 15 -14.801 CTCGAGAAAGCTCGCGAA\par + 16 -16.163 CTCGCGAATCTTCCGCGA\par + 17 -16.280 CTCGCGAAAGTTCTTCGG\par + 18 -15.598 CTCGGGAAACTTCGGGTC\par + 19 -17.721 TGCCAGAAGTTGCTAGCA\par + 20 -16.257 CTCGGGAACGTCCCAGAA\par + 21 -14.243 ATCCCGAAACTTCTAGTT\par + 22 -16.456 GTCCAGAATGTTTCTGAA\par + 23 -15.453 TTTCAGAAAATTCTAGTT\par + 24 -17.443 CCCAAGGACTTTCTCGAA\par + 25 -13.335 TTTTAGAATGTTCTAGAA\par + 26 -15.914 TTCTAGAACATTCGAAGA\par +Top score -12.227 Bottom score -20.897\par +Mean -16.119 Standard deviation 1.636\par +Mean minus 3.sd -21.028 Mean plus 3.sd -11.210\par +? Cutoff score (-999.00-9999.00) (-21.03) =\par +? Top score for scaling plots (-21.03-999.00) (-11.21) =\par +? Position to identify (0-18) (1) =\par +? Title=Heatshock weights 24-10-91\par +\pard \li1440\ri1500\sl220\keepn\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth ? Name for new weight matrix file=heatshock.wts\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 6.4\tab An example run of creating a weight matrix\par +\pard\plain \li1400\ri1500\sb300\sl220\box\brsp100\brdrth \f4\fs16 Motif search using weight matrix\par +\pard \li1400\ri1500\sl220\box\brsp100\brdrth Select operation\par +X 1 Use weight matrix\par + 2 Make weight matrix\par + 3 Rescale weight matrix\par +? Selection (1-3) (1) =\par +? Motif weight matrix file=heatshock.wts\par + Heatshock weights 24-10-91\par +? Cutoff score (-9999.00-9999.00) (-21.03) =\par +? Plot results (y/n) (y) =\par +\par + 619 -20.84 gctcggaagcttctgctc\par + 818 -20.74 ttggcgaagctttcaaag\par + 1190 -21.02 gccaggtaagtttcagac\par + 1601 -20.91 tttgcgactgttcggtaa\par + 2387 -20.24 cgctcgcagattctggac\par + 2534 -20.87 gccgagaagatcatcgaa\par + 2890 -16.38 ctcccggatgttctggag\par + 2989 -19.54 ctcgcgaaaatttctgct\par + 3451 -20.76 atcctggaagttccggtt\par + 6020 -20.73 tctcaggaactgctggaa\par + 6335 -20.51 gctgagaaattccgtgac\par + 7107 -20.31 ctctggtctggtcgagaa\par + 7117 -19.61 gtcgagaaaatccaggta\par +\pard \li1400\ri1500\sl220\keepn\box\brsp100\brdrth 7892 -20.18 cttccgaaagtgctgcat\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 6.5\tab Example run of a search using a weight matrix to produce text output.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.4\tab Using "hardwired" motif searches.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The program contains predefined motif definitions for the following\:\par +\pard \s4\qj\li1120\sa120\sl280 {\i E. coli} promoters\par +prokaryotic ribosome binding sites\par +mRNA splice junctions\par +eukaryotic ribosome binding sites\par +polyadenylation sites\par +\pard \s4\qj\sb240\sa120\sl280 All except the po +lyadenylation site, which is simply defined as an exact match to the string AATAAA, are represented as weight matrices. Each search is performed simply by the user selecting the appropriate option from the menu and each plots its results in its own graphic +s window. The ribosome binding site searches are reading frame specific and so they normally plot their results to fit nicely with the output from the "gene search by content" methods described in the chapter on finding genes. Likewise the splice junction +searches produce separate output for each of the three reading frames. Below, as an example of using the hardwired motifs, we show how to perform such a search.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.4.1\tab Searching for splice junctions\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Splice search using weight matrix". The program automatically reads in weight matrices that define the donor and acceptor sites and displays their titles.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Define "Donor cutoff score". The default is stored in the file.\par +3.\tab Define "Acceptor cutoff score". The default is stored in the file.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4. \tab Accept "Plot results". The alternative lists the results giving the position, score, matching sequence and reading frame. A typical plotted result appears in figure 6.6.\par +\pard\plain \qj\ri-100\sb240\sl480\keepn \f4\fs16 {{\pict\macpict\picw454\pich123 +04be00000000007b01c6001102ff0c00fffe0000002d8f9e002d8f9e00000000004e011f000000000001000a00000000004e011f0098802400000000004e011f0000000000000000002d8f9e002d8f9e000000010001000100000000000000000000000000439867000000010000ffffffffffff0001000000000000000000 +00004e011f00000000004e011f000002dd0006007fdfff00fc060040df000004060040df0000040a0040e9000020f80000040a0040e9000020f80000040c0040e9000020fa00022000040c0040e9000020fa0002200004110040eb0005200020000080fd0002200004170040fd000001f200071000200020000090fd000220 +0004170040fd000001f200071000600020080090fd0002200004170040fd000001f200071000600020080090fd0002200004180040fe00011001f2000712006000200c0090fd000224000406007fdfff00fc060040df0000040a0040ee000008f30000040a0040ee000008f30000040a0040ee000008f30000040a0040ee00 +0008f30000040a0040ee000008f30000040a0040ee000008f300000c0a0040ee000008f300000c0a0040ee000008f300000c0e0040ee000008fe000010f700000c180040f6000001fc0002010008fe000010fc000008fd00000c2002400004fd0005400010000001fc000601000808001010fc000008fe0001800c06007fdf +ff00fc060040df000004060040df0000040a0040fc000004e50000040a0040fc000004e50000040c0040fc000004e700020104040c0041fc000004e70002010404100041fc000004fe000008eb0002010404100041fc000004fe000008eb0002010404150041fc000004fe00010814f3000010fb00020104041a014180fd00 +0004fe0005081400400040f7000010fb00020904041b02498008fe000904400200081400400040f7000050fb000209040406007fdfff00fc060040df000004060040df000004060040df000004060040df000004060040df0000040a0040fe000010e30000040e0040fe000010f4000001f10000040e0040fe000010f40000 +01f10000040e0040fe000010f4000001f10000040e0040fe000018f4000001f1000004180040fe000018f60002080001fb00040800008001fc0000041d04400000081afd000005fc000308080001fb00040800008001fc00000406007fdfff00fc060040df000004060040df0000040a0040f8000008e90000040a0040f800 +0008e90000040a0040f8000008e90000040a0040f8000008e90000040e0040f8000008ee000004fd000004140040fa0002400008f6000002fa000004fd000004180040fe000040fe0002400008f6000002fa000004fd000004190040fe000040fe0002400008f600010a02fb000004fd000004220048fe000a402000004000 +4801000001fe0006408000000a0202fc000004fd00000406007fdfff00fc060040df000004060040df000004060040df000004060040df000004090040e2000340000004090040e20003400000040c0340000002e50003400000040c0340000002e50003400000040e0340000002e70005080040020004120340000002eb00 +0001fe0005080040020004120340080002eb000001fe00050800400200041b044008020280f6000040fd000008fd000001fe000508004002000406007fdfff00fc02dd000000ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 6.6\tab +Typical graphical output from using the hardwired splice junction search. The results are presented in a reading frame specific way so it shows, in the bottom three boxes, results for donor sites and in the top three boxes those for acceptor sit +es. In both cases the vertical ordering of the boxes is frame 0 at the bottom, frame 1 in +the middle and frame 2 at the top. For example there is a very strong peak corresponding to an acceptor in frame 1 that can be seen just over halfway along the sequence .\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 3.\tab Notes\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab +For this program a motif is a short segment of sequence of fixed length. More complex structures termed "patterns" which we define as sets of motifs separated by varying gaps, are covered in another chapter. The current chapter should be read before the + chapter on patterns. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab It is debateable whether the gain in sensitivity that is afforded by the use of a score matrix is of value for searching nucleotide sequences, however it is very important for protein sequences.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +The files of aligned sequences used to make weight matrices have the following format. Each sequence should be on a separate line. The sequence should start in column 2 and is terminated by a new line or a space. Anything after the space is treated as +a comment. The files can be created by previous searches or using an editor.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab The frequencies in the weight +matrix can be used in two ways to calculate scores for sequences. Some users prefer to add the frequencies to give a total score, and others to multiply them by summing their logs. If we regard the frequencies as probabilities then multiplication seems the + correct procedure. The user chooses which method will be employed when the weight matrix is created, however the choice can be overridden when the matrix is used. If multiplication is selected then all results will presented as sums of logs.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Masking th +e weight matrix is particularly useful in cases where a limited number of examples of a motif are available, or when the motif may have several components. In the first case the limited number of examples may make the matrix unrepresentative of the motif b +ecause the bases in the unconserved positions may bias the results of searches. When a large number of examples is available to create the matrix, the unconserved positions should tend towards equal base composition and hence have no influence on the overa +ll score. We stated that a motif might have several components\: for example a motif might have both structural and specificity components. We may want to separate out the two parts and masking provides such a facility.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab +The weight matrix handling routine contains a further option "Rescale weight matrix". If the user has edited a weight matrix to change the frequency values this provides a way of selecting a new cutoff score. It allows users to read in a set of aligned + sequences and a weight matrix and to apply the matrix to the set of sequences to see the range of scores achieved. A new weight matrix file containing the selected cutoff score is written to disk.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab The program also contains a set of routines identical to those used to create and search for nucleotide weight matrices, but which deal instead with dinucleotide weight matrices. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 8.\tab The reader is reminded that most options in the program, if selected when in "execute without dialogue" mode, will automatically use a set of defaults and produce a +result with little or no user input. Most motif searches require far less user input than that shown above, where we have tried to show the scope of the methods.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 9.\tab +Although the program contains hardwired motifs we expect most sites that use the programs to accumulate their own libraries of motifs and patterns, which users can employ by simply knowing the names of the corresponding files.\par +\pard\plain \s5\sa60\sl320\tx560 \b\f20\fs28 \page 4.\tab References\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Staden, R. 1984. Computer methods to locate signals in nucleic acid sequences. {\i Nucl. Acids Res}. {\b 12}\:521-538.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Staden, R. 1985. Computer methods to locate genes and signals in nucleic acid sequences. (in) {\i Genetic Engineering, Principle and Methods, }Setlow J.K., Hollaender A., (eds.), {\b 7}\: +67-114, (Plenum Press, New York).\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Staden, R. 1988. Methods to define and locate patterns of motifs in sequences. {\i CABIOS} {\b 4 (1)}\:53-60.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Staden, R. 1990. Searching for patterns in protein and nucleic acid sequences. (in) {\i Methods in Enzymology} R.F. Doolittle (ed.), {\b 183}\:193-211 (Academic Press, New York).\par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 7. Using Patterns to Analyse Nucleic Acid Sequences\par +\pard\plain \s5\sb200\sa60\sl320\tx560 \b\f20\fs28 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +2.\tab Methods\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.1\tab Creating a pattern file containing an exact match motif and weight matrix motif.\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.2\tab Searching a sequence using a pattern file\par +2.3\tab Comparing a sequence against a library of patterns\par +2.4\tab Searching sequence libraries for patterns\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Notes\par +4.\tab References\par +\pard\plain \s5\sb200\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 Here we describe one of the most powerful facilities provided by the program NIP\: the ability to define and search for complex patterns of motifs (1-3). +In another chapter we give details of seaching for individual motifs but here we show how to create patterns and libraries of patterns and to use them to search single sequences and sequence libraries. Once a pattern has been defined and stored in a file +it can used to search any sequence. In addition if users want to routinely screen sequences against libraries of patterns this can be achieved by use of files of file names. The program can produce several alternative forms of output. It will display the s +egment of sequence matching each individual motif in the pattern, display all the sequence between and including the two outermost motifs, produce a description of the match in the form of an EMBL feature table, or draw a simple graphical plot.\par +\pard \s4\qj\sa120\sl280 At the end of the chapter we describe how a related program NIPL is used to search libraries of sequences to find patterns. NIPL is capable of producing alignments of sequence families.\par +\pard \s4\qj\sa120\sl280 Patterns are defined as sets of motifs with variable spacing. Each motif in a pat +tern can be defined using any of several methods, and their positions relative to one other are defined in terms of minimum and maximum separations. In addition, by the use of logical operators, each motif can be declared to be essential (the AND operator) +, optional (the OR operator), or forbidden (the NOT operator). The following methods (termed "classes" by the program) for defining motifs are provided\: + 1) exact match to a short sequence; 2) percentage match to a short sequence; 3) match to a short sequen +ce using a score matrix and cutoff score; 4) match to a weight matrix; 5) match to the complement of a weight matrix; 6) inverted repeat or stem-loop; 7) exact match to a short sequence with a defined step; 8) direct repeat. Classes 1, 2 , 3 and 7 permit t +he use of IUB redundancy codes.\par +\pard \s4\qj\sa120\sl280 The motifs in a pattern are numbered sequentially and motif spacing is defined in the following way. When a new motif is added to a pattern the user specifies the "Reference motif" by its number and then a "Relative start po +sition". The "Relative start position" is defined by taking the first base of the "Reference motif" as position 1, the next as 2, and so on. Then the user defines the allowed variation in the spacing by specifying the "Number of extra positions". Notice th +at the position of a motif can be defined relative to any other motif, and that a negative "Relative start position" declares the motif to be to the left of its "Reference motif".\par +\pard \s4\qj\sa120\sl280 The probability of finding each individual motif in the current sequence, th +e product of the probabilities for all the motifs in a pattern "Probability of finding pattern", and the "Expected number of matches" is calculated and displayed by the program. In addition to the cutoffs used for the individual motifs, users can apply two + pattern cutoffs\: "Maximum pattern probability" and "Minimum pattern score".\par +Below we describe\: how to create a pattern; how to use a pattern file to search a sequence; how to use a "File of pattern file names" to search a sequence for a whole library of +patterns. To describe how to create a pattern file we first show all the steps to make one containing two motifs, and then, to save space, the parts specific to the individual motif types are sketched in the notes section.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 2. Methods\par +\pard\plain \s6\fi-560\li560\sb240\sa60\sl280\tx560\tx860 \b\f20 2.1\tab Creating a pattern file containing an exact match motif and weight matrix motif.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Pattern searcher".\par +2.\tab Select "Pattern definition mode" as "Use keyboard".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Select "Results display mode" as "Motif by motif". The alternatives are listed in the introduction.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Select "Motif definition mode" as "Exact match".\par +5.\tab Define "Motif name". Each motif can be given an 8 character name.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Define "String". Type in the sequence of the motif. The program will display the probability of finding the motif.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Select "Motif definition mode" as "Weight matrix".\par +8.\tab Define "Motif name".\par +9.\tab Select "Logical operator" as "AND". The alternatives are "OR" and "NOT".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 10.\tab Select "Number of reference motif". At this stage the only choice is 1 and this is the default.\par +11.\tab Define "Relative start position". The base position relative to the "Reference motif". See the introduction.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 12.\tab Define "Number of extra positions".\par +13.\tab Define "Weight matrix file name". Type the name of the file containing the weight matrix.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \tab The program now cycles round to step 7 and all subsequent passes round the loop to add further motifs to the pattern would differ only in the details for the different motif "classes".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 14.\tab Select "Pattern complete"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 15.\tab Accept "Save pattern in a file". The alternative does not save the pattern and so it can only be used once on the current sequence.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 16.\tab Define "Pattern definition file". Give a name for the new file.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 17. \tab +"Define "Pattern title". All patterns can have a 60 character title that can be displayed when the pattern file is read and the sequence searched. The program will now display a detailed textual description of the pattern, the "Probability of finding +the pattern" and the "Expected number of matches".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 18.\tab Define "Maximum pattern probability". Yes maximum\: any match with a greater probability of being found will be rejected. If no value is specified the search will be quicker (see notes).\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 19.\tab +Define "Minimum pattern score". A minimum pattern score only makes sense if all the motifs in the pattern are defined with compatible scoring methods. For example percentage matches and weight matrices using sums of logs are incompatible. Searching wil +l now commence and any matches displayed using the chosen method. A worked example of creating such a pattern and performing a search is shown in figure 7.1, and the actual pattern file is shown in figure 7.2.\par +\pard\plain \li1360\ri1300\sb200\sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth \f4\fs16 Pattern searcher\par +\pard \li1360\ri1300\sl220\brdrl\brsp100\brdrth \brdrr\brsp100\brdrth Select pattern definition mode\par +X 1 Use keyboard \par + 2 Use pattern file \par + 3 Use file of pattern file names\par +? Selection (1-3) (1) =\par +Select results display mode\par +X 1 Motif by motif \par + 2 Inclusive \par + 3 Graphical \par + 4 EMBL feature table \par +? Selection (1-4) (1) =\par +Select motif definition mode\par +X 1 Exact match \par + 2 Percentage match \par + 3 Cut-off score and score matrix \par + 4 Cut-off score and weight matrix\par + 5 Complement of weight matrix \par + 6 Inverted repeat or stem-loop \par + 7 Exact match, defined step \par + 8 Direct repeat \par + 9 Pattern complete \par +? Selection (1-9) (1) =\par +? Motif name=T run\par +? String=TTTTT\par +Probability of score 5.0000 = 0.870E-03\par +Select motif definition mode\par +X 1 Exact match \par + 2 Percentage match \par + 3 Cut-off score and score matrix \par + 4 Cut-off score and weight matrix\par + 5 Complement of weight matrix \par + 6 Inverted repeat or stem-loop \par + 7 Exact match, defined step \par + 8 Direct repeat \par + 9 Pattern complete \par +? Selection (1-9) (1) =4\par +? Motif name=heat\par +Select logical operator\par +X 1 And \par + 2 Or \par + 3 Not \par +? Selection (1-3) (1) =\par +? Number of reference motif (1-1) (1) =\par +? Relative start position (-1000-1000) (6) =10\par +? Number of extra positions (0-1000) (0) =20\par +? Weight matrix file name=heatshock.wts\par + Heatshock weights 18-12-90 \par +Probability of score -21.0280 = 0.117E-02\par +Select motif definition mode\par + 1 Exact match \par + 2 Percentage match \par + 3 Cut-off score and score matrix \par +X 4 Cut-off score and weight matrix\par + 5 Complement of weight matrix \par + 6 Inverted repeat or stem-loop \par + 7 Exact match, defined step \par + 8 Direct repeat \par + 9 Pattern complete \par +? Selection (1-9) (4) =9\par +? Save pattern in a file (y/n) (y) =\par +? Pattern definition file=_paper.pat\par +? Pattern title=demo pattern\par +Pattern description\par +\par +demo pattern \par +Motif 1 named T run is of class 1\par +Which is an exact match to the string\par +TTTTT\par +Motif 2 named heat is of class 4\par +Which is a match to a weight matrix with score -21.028\par +and the 5 prime base can take positions 10 to 30\par +relative to the 5 prime end of motif 1\par +It is anded with the previous motif.\par +Probability of finding pattern = 0.1015E-05\par +Expected number of matches = 0.1734E+00\par +? Maximum pattern probability (0.00-1.00) (1.00) =\par +? Minimum pattern score (-9999.00-9999.00) (-9999.00) =\par +Working\par +Match\par + 505 T run \par + ttttt\par + 528 heat \par + ttaaagaaagttttatac\par +Total matches found 1\par +\pard \li1360\ri1300\sl220\keepn\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth Minimum and maximum observed scores -15.34 -15.34\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 7.1\tab Worked example of creating a simple pattern and performing a search.\par +\pard\plain \li2380\ri2520\sb300\sl220\box\brsp100\brdrth \f4\fs16 demo pattern \par +\pard \li2380\ri2520\sl220\box\brsp100\brdrth A1 T run Class \par + TTTTT\par + @ End of string\par + A4 heat Class \par + 1 Relative motif\par + 10 Relative start position\par + 20 Number of extra positions\par +\pard \li2380\ri2520\sl220\keepn\box\brsp100\brdrth heatshock.wts\par +\pard\plain \s8\qj\fi-1140\li1140\sb80\sa120\sl240\tx1140 \f21\fs20 Figure 7.2\tab The pattern file created by the work shown in figure 7.1.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 \page 2.2\tab Searching a sequence using a pattern file\par +\pard\plain \s7\qj\fi-560\li560\sb160\sa120\sl280\tx560 \f20 1.\tab Select "Pattern searcher"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Select "Pattern definition mode" as "Use pattern file".\par +3.\tab Select "Results display mode" as "Inclusive"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Pattern definition file". Type the name of the file containing the pattern. The pr +ogram will read the file then display its title, a detailed textual description of the pattern, the "Probability of finding the pattern", and the "Expected number of matches".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Define "Maximum pattern probability". \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Define "Minimum pattern score". Searching will now commence and any matches displayed using the chosen method. A worked example, using the pattern file created in figure 7.1 is shown in figure 7.3.\par +\pard\plain \li1300\ri1320\sb300\sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth \f4\fs16 Pattern searcher\par +\pard \li1300\ri1320\sl220\brdrl\brsp100\brdrth \brdrr\brsp100\brdrth Select pattern definition mode\par +X 1 Use keyboard \par + 2 Use pattern file \par + 3 Use file of pattern file names\par +? Selection (1-3) (1) =2\par +? Pattern definition file=_paper.pat\par +Select results display mode\par +X 1 Motif by motif \par + 2 Inclusive \par + 3 Graphical \par + 4 EMBL feature table \par +? Selection (1-4) (1) =2\par +Probability of score 5.0000 = 0.870E-03\par + Heatshock weights 18-12-90 \par +Probability of score -21.0280 = 0.117E-02\par +\par +Pattern description\par +\par + demo pattern \par +Motif 1 named T run is of class 1\par +Which is an exact match to the string\par +TTTTT\par +Motif 2 named heat is of class 4\par +Which is a match to a weight matrix with score -21.028\par +and the 5 prime base can take positions 10 to 30\par +relative to the 5 prime end of motif 1\par +It is anded with the previous motif.\par +Probability of finding pattern = 0.1015E-05\par +Expected number of matches = 0.1734E+00\par +? Maximum pattern probability (0.00-1.00) (1.00) =\par +? Minimum pattern score (-9999.00-9999.00) (-9999.00) =\par +Working\par + 505 T run \par + tttttgatgcttgactctaagccttaaagaaagttttatac\par +Total matches found 1\par +\pard \li1300\ri1320\sl220\keepn\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth Minimum and maximum observed scores -15.34 -15.34\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 7.3\tab Worked example of using a pattern file as input.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 \page 2.3\tab Comparing a sequence against a library of patterns\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This mode of operation allows a sequence to be searched, in turn, for any number of patterns each stored in a separate pattern file. The names of the files containing the individual patterns must be stored in a simple text file. This file is called "a file + of pattern file names" and its name is the only user input required to define the search.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Pattern searcher"\par +2.\tab Select "Pattern definition mode" as "Use file of pattern file names".\par +3.\tab Select "Results display mode" as "Inclusive"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab +Define "File of pattern file names". Type the name of the file containing the list of pattern file names. The program will read the file and then, in turn, all the pattern files it names. Each of these patterns will be compared against the current seque +nce but only those that give matches will produce any output. The pattern title and each match will be displayed.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.4\tab Searching sequence libraries for patterns\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The program NIPL can be used to search sequence libraries for patterns. Its use is similar to the pattern search routine described above, except that it does not have the facility for creating pattern files, so they must be created beforehand using NIP. In + addition to its obvious application of finding new occurrences of patterns or checking on their frequency it is a usef +ul way of obtaining sequence alignments. It can restrict its search to a list of named entries or can search all but those on a list of entries. It can restrict its output to showing the highest scoring match in each sequence, but by default it will show a +ll matches.\par +\pard \s4\qj\sa120\sl280 +Of its modes of output, two require further description. The first "Padded sections" creates a new file for each match. The file will contain the sequence between and including the two outermost motifs in the pattern. It will be gapped to the f +urthest extent defined by the pattern, which means that if all the files were subsequently written one above the other all the motifs in the pattern would be exactly aligned, with the sections between them containing the requisite numbers of padding charac +ters. The second such mode of output is called "Complete padded sequences". Here the user must know the maximum distance between the leftmost motif and the start of all the sequences that match. A trial run in which only the positions of matches are report +ed is usually required. The user gives this maximum distance to the program. The program then writes a new file containing the full length of all matching sequences, again maximally gapped (including their left ends) so that they would all align if written + above one another. For both of these modes of output the files created are named "entryname" where "entryname" is the name given to the sequence in the sequence library. These modes are best used with the option "Report all matches" rejected, so that only + the best match for each sequence is reported. The sequences can be lined up using the sequence assembly program SAP.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select NIPL.\par +2.\tab Define "Name for results file."\par +3.\tab Select a library.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab +Select "Search whole library". The alternatives are "Search only a list of entries" and "Search all but a list of entries". The files containing the list of entries should contain one entry name per line, left justified.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Select "Results display mode" as "Inclusive". The alternatives include "Motif by motif", "Scores only", "Complete padded sequences" and "Padded sections".\par +6.\tab Accept "Report all matches". The alternative only shows the best match for each sequence.\par +7.\tab Define "Pattern definition file". The name of the file containing the pattern created using NIP. \par +\tab The program displays a textual description of the pattern and the expected number of matches per 1000 residues assuming an average nucleic acid composition.\par +8.\tab Define "Maximum pattern probability". The program will run much more quickly if none is given.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 9.\tab Define "Minimum pattern score".\par +\pard\plain \s4\qj\sa120\sl280 \f20 The search will start.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 3.\tab Notes\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab +The "exact match" motif class requires a consensus sequence. The "percentage match" motif class requires a consensus sequence and a cutoff score. The "score matrix" motif class requires a consensus sequence and a cutoff score. The "weight matrix" searc +h and the "complement of a weight matrix" only require the name of the file containing the matrix. The "inverted repeat" or "stem-loop" requires a stem length, minimum and maximum loop sizes, + and a cutoff score using scores A-T = G-C = 2, G-T = 1. Note that if the user defines an inverted repeat as a "Reference motif" the "Relative position" can be defined from either its 5' or 3' ends. The "direct repeat" motif class requires a repeat length +, the minimum and maximum gap between the two occurrences of the repeat, and a minimum score.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab The motif class "Exact match, defined step" is rarely used. A typical use might be to find a start codon followed, for some minimum distance, by no stop codons + in the same reading frame. The step would have the value 3 to keep the reading frame the same as that of the start codon, and the stop codon searches would be included using the NOT operator.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +The details of the probabilty calculations are outside the scope of this article. They are quite rapid and are essential both for assessing the statistical significance of any matches found and for allowing meaningful cutoffs to be applied to patterns. +Obviously, in general, cutoff scores are inappropriate for patterns containing a mixture of motif classes.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab +The program calculates the "Probability of finding the pattern" and the "Expected number of matches". The first figure is actually the product of the individual motif probabilities but the latter figure is more useful because it takes into account the a +llowed variation in spacing between motifs and the length of the current sequence. In both cases the composition of the current sequence is also used so that different probabilities would be calculated for other sequences.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab +The pattern definition system is very flexible. Assume that a laboratory has a large library of patterns stored in its computer. Different groups or users may want to screen their sequences against different subsets of a pattern library. Each group ther +efore uses its own "File of pattern file names" which contains only the names of the pattern files that are relevant to their sequences. Of course a pattern may contain only one motif. Hence a library of patterns can include both simple and comp +lex patterns. In the same way a laboratory may have a large library of weight matrices defining different motifs and different users may want to combine them in different ways to produce their own patterns. \par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 4.\tab References\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Staden, R. 1988. Methods to define and locate patterns of motifs in sequences. {\i CABIOS} {\b 4(1)}\:53-60.\par +2.\tab Staden, R. 1989. Methods for calculating the probabilities of finding patterns in sequences. {\i CABIOS} {\b 5(2)}\:89-96.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Staden, R. 1990. Searching for patterns in protein and nucleic acid sequences. (in) {\i Methods in Enzymology} R.F. Doolittle (ed.), {\b 183}\:193-211 (Academic Press, New York).\par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 8. Searching for Restriction Sites\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +2.\tab Methods\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.1\tab Search for restriction sites and list them enzyme by enzyme\par +2.2\tab Search for restriction sites and list them by position\par +2.3\tab Search for restriction sites and list their names above the sequence\par +2.4\tab Search for restriction sites and plot their positions\par +2.5\tab Find restriction enzymes that cut infrequently\par +2.6\tab Producing a back translation from a protein sequence\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Notes\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The program NIP contains a routine for finding and displaying the positions of the cut sites of restriction enzyme recognition sequences. Linear or circular sequences can be searched and the results can be listed in various forms or displayed graphically. +The recognition sequences to be searched for can be typed on the keyboard or read from files. The format of these files is given in note 1. At the end of the chapter we also describe how to pro +duce back translations of protein sequences so that these routines can be used to search them for restriction sites.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 2.\tab Methods\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.1\tab Search for restriction enzyme sites and list them enzyme by enzyme\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Search".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Select "Input source" as "All enzymes file". A number of standard files are available and users may also have their own.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Accept "Search for all names". \par +4.\tab Select "Order results enzyme by enzyme".\par +5.\tab Accept "List matches".\par +6.\tab Accept "The sequence is linear". The alternative is circular.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Accept "Search for definite matches". The alternative is to search for possible matches in a sequence containing IUB redundancy codes.\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The results will then appear in the form shown in figure 8.1 Each match is numbered and its enzyme name given, followed by the matching sequence with the cut site indicated by a ' symbol. The position of the cut site is given followed by the length of the +potential fragment ending at that site, followed by a list of fragments sizes sorted on length.\par +\pard\plain \li1160\ri1380\sl220\box\brsp100\brdrth \f4\fs16 Matches found= 3\par +\pard \li1160\ri1380\sl220\box\brsp100\brdrth Name Sequence Position Fragment length\par + 1 AccII cg'cg 313 312 51\par + 2 AccII cg'cg 364 51 188\par + 3 AccII cg'cg 552 188 312\par + 449 449\par +Matches found= 6\par + Name Sequence Position Fragment length\par + 1 AciI cc'gc 503 502 12\par + 2 AciI gc'gg 553 50 12\par + 3 AciI gc'gg 714 161 50\par + 4 AciI gc'gg 872 158 105\par + 5 AciI gc'gg 884 12 158\par + 6 AciI cc'gc 896 12 161\par + 105 502\par +Matches found= 3\par + Name Sequence Position Fragment length\par + 1 AcyI gg'cgtc 698 697 5\par + 2 AcyI gg'cgtc 765 67 67\par +\pard \li1160\ri1380\sl220\keepn\box\brsp100\brdrth 3 AcyI ga'cgcc 996 231 231\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 8.1\tab Typical output from "List enzyme by enzyme".\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.2\tab Search for restriction enzyme sites and list them by position\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Search".\par +2.\tab Select "Input source" as "All enzymes file". \par +3.\tab Accept "Search for all names". \par +4.\tab Select "Order results by position".\par +5.\tab Accept "List matches". \par +6.\tab Accept "The sequence is linear".\par +7.\tab Accept "Search for definite matches". \par +\pard\plain \s4\qj\sa120\sl280 \f20 The results will then appear in the form shown in figure 8.2 Each match is numbered and its enzyme name given, followed b +y the matching sequence with the cut site indicated by a ' symbol. The position of the cut site is given followed by the length of the potential fragment ending at that site.\par +\pard\plain \s6\fi-540\li560\sb240\sa60\sl280\tx560 \b\f20 2.3\tab Search for restriction enzyme sites and list their names above the sequence\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Search".\par +2.\tab Select "Input source" as "All enzymes file". \par +3.\tab Accept "Search for all names". \par +4.\tab Select "Show names above the sequence".\par +5.\tab Reject "Hide translation".\par +6.\tab Accept "Use 1 letter codes".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Define "Line length". This is the number of bases that will appear on each line of output. It must be a multiple of 30. \par +\pard\plain \li1640\ri1720\sl220\pagebb\box\brsp100\brdrth \f4\fs16 Name Sequence Position Fragment length\par +\pard \li1640\ri1720\sl220\box\brsp100\brdrth 1 HapII c'cgg 2 1\par + 2 HpaII c'cgg 2 0\par + 3 MspI c'cgg 2 0\par + 4 MseI t'taa 14 12\par + 5 HincII gtt'aac 15 1\par + 6 HindII gtt'aac 15 0\par + 7 HpaI gtt'aac 15 0\par + 8 DsaV 'ccagg 23 8\par + 9 EcoRII 'ccagg 23 0\par +10 TspAI 'ccagg 23 0\par +11 ApyI cc'agg 25 2\par +12 BstNI cc'agg 25 0\par +13 MvaI cc'agg 25 0\par +14 ScrFI cc'agg 25 0\par +15 MaeIII 'gttac 47 22\par +16 BsrI actggt' 49 2\par +17 MseI t'taa 55 6\par +18 MaeII a'cgt 63 8\par +19 SfaNI gcatcaacaa'gata 86 23\par +\pard \li1640\ri1720\sl220\keepn\box\brsp100\brdrth 20 MaeII a'cgt 91 5\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa400\sl240\tx1140 \f21\fs20 Figure 8.2\tab Typical output from "List by position".\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 8.\tab Accept "The sequence is linear".\par +9.\tab Accept "Search for definite matches". \par +\pard\plain \s4\qj\sa120\sl280 \f20 The results will then appear in the form shown in figure 8.3 The sequence is listed with a 3 phase translation underneath and every tenth base numbered. Above the sequence the positions of the cut sites of res +triction enzymes are marked.\par +\pard\plain \s6\sb160\sa60\sl280\tx560\tx860 \b\f20 2.4\tab Search for restriction enzyme sites and plot their positions \par +\pard\plain \s7\qj\fi-560\li560\sa80\sl260\tx560 \f20 1.\tab Select "Search".\par +2.\tab Select "Input source" as "All enzymes file". \par +3.\tab Accept "Search for all names". \par +4.\tab Select "Order results by position".\par +5.\tab Reject "List matches". \par +6.\tab Accept "The sequence is linear".\par +7.\tab Accept "Search for definite matches".\par +\pard\plain \s4\qj\sa80\sl260 \f20 The results will then appear in the form shown in figure 8.4. Each enzyme that has a match is named at the left edge of the display and its cut sites are marked by short +vertical lines. If the display window fills up the bell will ring. Users may then take a screen dump before typing return. The program then displays the message " ? Restart plotting from bottom of frame". To do so type return. To quit type !.\par +\pard\plain \li1200\ri1240\sl220\pagebb\box\brsp100\brdrth \f4\fs16 Search for restriction enzyme sites\par +\pard \li1200\ri1240\sl220\box\brsp100\brdrth Select operation\par +X 1 Search\par + 2 List enzyme file\par + 3 Clear text\par + 4 Clear graphics\par +? Selection (1-4) (1) =\par +Select input source\par + 1 All enzymes file\par +X 2 Six cutter file\par + 3 Four cutter file\par + 4 Personal file\par + 5 Keyboard\par +? Selection (1-5) (2) =1\par +? Search for all names (y/n) (y) =\par + Select results display mode\par +X 1 Order results enzyme by enzyme\par + 2 Order results by position\par + 3 Show only infrequent cutters\par + 4 Show names above the sequence\par +? Selection (1-4) (1) =4\par +? Hide translation (y/n) (y) =n\par + ? Use 1 letter codes (y/n) (y) =\par + ? Line length (30-90) (60) =\par +? The sequence is linear (y/n) (y) =\par + ? Search for definite matches (y/n) (y) =\par +\par + HapII\par + HpaII\par + MspI MseI\par + . .HincII\par + . .HindII\par + . .HpaI DsaV\par + . .. EcoRII\par + . .. TspAI\par + . .. . ApyI\par + . .. . BstNI\par + . .. . MvaI\par + . .. . ScrFI MaeIII\par + . .. . . . BsrI MseI\par +ccggttagactgttaacaacaaccaggttttctactgatataactggttacatttaacgc\par + 10 20 30 40 50 60\par + P V R L L T T T R F S T D I T G Y I * R\par + R L D C * Q Q P G F L L I * L V T F N A\par +\pard \li1200\ri1240\sl220\keepn\box\brsp100\brdrth G * T V N N N Q V F Y * Y N W L H L T P\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa400\sl240\tx1140 \f21\fs20 Figure 8.3\tab Typical dialogue and output for a "Names above the sequence" search.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.5\tab Finding restriction enzymes that cut infrequently\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Search".\par +2.\tab Select "Input source" as "All enzymes file". \par +3.\tab Accept "Search for all names". \par +4.\tab Select "Show only infrequent cutters".\par +5.\tab Define "Maximum number of cuts".\par +6.\tab Accept "The sequence is linear".\par +\pard\plain \li160\ri200\sl220\keepn\box\brsp100\brdrth \f4\fs16 {{\pict\macpict\picw430\pich254 +0b99ffffffff00fd01ad1101a0008201000affffffff00fd01ad090000000000000000310000002400fa01ac9800240000000000b7011f0000000000b7011f0000002400fa01ac000102dd001402000080fd000001f00002100040fc000010ff001402000080fd000001f00002100040fc000010ff001402000080fd000001 +f00002100040fc000010ff001402000080fd000001f00002100040fc000010ff001402000080fd000001f00002100040fc000010ff001402000080fd000001f00002100040fc000210000006007fdfff00fc06f5000020ea0006f5000020ea0006f5000020ea0006f5000020ea0006f5000020ea0006f5000020ea0006007f +dfff00fc06fe000008e10006fe000008e10006fe000008e10006fe000008e10006fe000008e10006007fdfff00fc06fe000008e10006fe000008e10006fe000008e10006fe000008e10006fe000008e10006007fdfff00fc040020de00040020de00040020de00040020de00040020de00040020de0006007fdfff00fc1402 +000080fd000001f00002100040fc000010ff001402000080fd000001f00002100040fc000010ff001402000080fd000001f00002100040fc000010ff001402000080fd000001f00002100040fc000010ff001402000080fd000001f00002100040fc000210000006007fdfff00fc06fb000004e40006fb000004e40006fb00 +0004e40006fb000004e40006fb000004e40006fb000004e40006007fdfff00fc0af8000080fe000080eb000af8000080fe000080eb000af8000080fe000080eb000af8000080fe000080eb000af8000080fe000080eb0006007fdfff00fc040020de00040020de00040020de00040020de00040020de00040020de0006007f +dfff00fc0602000040e0000602000040e0000602000040e0000602000040e0000602000040e00006007fdfff00fc0602000040e0000602000040e0000602000040e0000602000040e0000602000040e00006007fdfff00fc0602000040e0000602000040e0000602000040e0000602000040e0000602000040e00006020000 +40e00006007fdfff00fc06eb000040f40006eb000040f40006eb000040f40006eb000040f40006eb000040f40006007fdfff00fc06eb000040f40006eb000040f40006eb000040f40006eb000040f40006eb000040f40006eb000040f40006007fdfff00fc06eb000010f40006eb000010f40006eb000010f40006eb000010 +f40006eb000010f40006007fdfff00fc040020de00040020de00040020de00040020de00040020de00040020de0006007fdfff00fc06eb000008f40006eb000008f40006eb000008f40006eb000008f40006eb000008f40006007fdfff00fc06fe000020e10006fe000020e10006fe000020e10006fe000020e10006fe0000 +20e10006fe000020e10006007fdfff00fc06fe000020e10006fe000020e10006fe000020e10006fe000020e10006fe000020e10006007fdfff00fc06fe000020e10006fe000020e10006fe000020e10006fe000020e10006fe000020e10006007fdfff00fc06eb000008f40006eb000008f40006eb000008f40006eb000008 +f40006eb000008f40006eb000008f40006007fdfff00fc06eb000010f40006eb000010f40006eb000010f40006eb000010f40006eb000010f40006007fdfff00fc06fe000008e10006fe000008e10006fe000008e10006fe000008e10006fe000008e10006007fdfff00fc06fe000008e10006fe000008e10006fe000008e1 +0006fe000008e10006fe000008e10006fe000008e10006007fdfff00fc06fa000080e50006fa000080e50006fa000080e50006fa000080e50006fa000080e50006007fdfff00fc06eb000008f40006eb000008f40006eb000008f40006eb000008f40006eb000008f40006eb000008f40006007fdfff00fc06fe000008e100 +06fe000008e10006fe000008e10006fe000008e10006fe000008e10006007fdfff00fc06fe000008e10006fe000008e10006fe000008e10006fe000008e10006fe000008e10006fe000008e10006007fdfff00fc02dd00a10096000c010000000200000000000000a1009a0008fffd00000011000001000a00020000000e00 +252c000800140554696d65730300140d00092e0004000001002b010b055472753949a00097a10096000c010000000200000000000000a1009a0008fffd00000011000001000a000c0000001800252a0a055366614e49a00097a10096000c010000000200000000000000a1009a0008fffd00000011000001000a0014000000 +2000252a08055363724649a00097a10096000c010000000200000000000000a1009a0008fffd00000011000001000a001c0000002800252a08044d766149a00097a10096000c010000000200000000000000a1009a0008fffd00000011000001000a00260000003200252a0a044d737049a00097a10096000c010000000200 +000000000000a1009a0008fffd00000011000001000a002e0000003a00252a08044d736549a00097a10096000c010000000200000000000000a1009a0008fffc00000011000001000a00370000004300252a09064d6165494949a00097a10096000c010000000200000000000000a1009a0008fffd00000011000001000a00 +400000004c00252a09054d61654949a00097a10096000c010000000200000000000000a1009a0008fffc00000011000001000a00490000005500252a09054d70614949a00097a10096000c010000000200000000000000a1009a0008fffc00000011000001000a00510000005d00252a08044d706149a00097a10096000c01 +0000000200000000000000a1009a0008fffc00000011000001000a00590000006500252a080648696e644949a00097a10096000c010000000200000000000000a1009a0008fffc00000011000001000a00630000006f00252a0a0648696e634949a00097a10096000c010000000200000000000000a1009a0008fffc000000 +11000001000a006b0000007700252a080648696e503149a00097a10096000c010000000200000000000000a1009a0008fffc00000011000001000a00750000008100252a0a0548696e3649a00097a10096000c010000000200000000000000a1009a0008fffc00000011000001000a007d0000008900252a080448686149a0 +0097a10096000c010000000200000000000000a1009a0008fffc00000011000001000a00870000009300252a0a054861704949a00097a10096000c010000000200000000000000a1009a0008fffc00000011000001000a008f0000009b00252a08054861654949a00097a10096000c010000000200000000000000a1009a00 +08fffd00000011000001000a0098000000a400252a090645636f524949a00097a10096000c010000000200000000000000a1009a0008fffc00000011000001000a00a1000000ad00252a090745636c31333649a00097a10096000c010000000200000000000000a1009a0008fffc00000011000001000a00a9000000b50025 +2a080444736156a00097a10096000c010000000200000000000000a1009a0008fffd00000011000001000a00b2000000be00252a090444646549a00097a10096000c010000000200000000000000a1009a0008fffd00000011000001000a00ba000000c600252a080443666f49a00097a10096000c01000000020000000000 +0000a1009a0008fffc00000011000001000a00c3000000cf00252a09054273744f49a00097a10096000c010000000200000000000000a1009a0008fffd00000011000001000a00cc000000d800252a09054273744e49a00097a10096000c010000000200000000000000a1009a0008fffd00000011000001000a00d4000000 +e000252a080442737249a00097a10096000c010000000200000000000000a1009a0008fffd00000011000001000a00de000000ea00252a0a084273703134334949a00097a10096000c010000000200000000000000a1009a0008fffd00000011000001000a00e6000000f200252a08054273694c49a00097a10096000c0100 +00000200000000000000a1009a0008fffd00000011000001000a00f0000000fc00252a0a0441707949a00097a00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa400\sl240\tx1140 \f21\fs20 Figure 8.4\tab Typical output from "Plot positions".\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 7.\tab Accept "Search for definite matches". \par +\pard\plain \s4\qj\sa120\sl280 \f20 The names and number of cut sites of all enzymes that cut less than or equal to the "Maximum number of cuts" will then be displayed.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.6\tab Producing a back translation from a protein sequence \par +\pard\plain \s4\qj\sa120\sl280 \f20 +The routine for producing back translations is contained in the program PIP. It back translates protein sequences into DNA using the standard genetic code. The translation can use either the IUB symbols or a set of codon preferences. If a set of codon pre +ferences is used they must conform to the format of codon tables pr +oduced by the nucleotide interpretation program, and the back translation will contain the favoured codons. If, for any amino acid there is no favoured codon, the IUB symbols will be employed. The program will plot the redundancy along the sequence and hen +ce can be used to find the best sequences to use as primers. The DNA sequence can be saved to a file and analysed using the nucleotide analysis program. \par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Back translate".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Accept "No codon preference". The alternative will cause the program to ask for "File name of codon table", which should be in the same format as those created by the nucleotide interpretation program. +\par +3.\tab Reject "Plot redundancy". The alternative will ask for a window length to use for the plot. The window length is in codons. A plot will appear in which the best primers are sited at the peaks and the worst at the troughs.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Accept "Save DNA to disk"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Define "File name for DNA sequence". This file can later be read into program NIP and all the searches described above employed.\par +\pard\plain \s5\sa60\sl320\tx560 \b\f20\fs28 \page 3.\tab Notes\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab +The file containing the definitions of the restriction enzymes names and their recognition sequences uses the standard IUB redundancy symbols and has the following format. Each name is followed by a /, then each of its recognition sequences is followed +by a /. The last recognition sequence for each enzyme is followed by //. The cut sites should be indicated by a '. If the cut site is not contained in the recognition sequence, the recognition sequence should be extended by sufficent N symbo +ls. For example the two lines from the standard file shown below define the enzymes Alw21I and Alw26I. These files are kindly updated each month by Dr. Rich Roberts.\par +\pard \s7\qj\li1720\sa120\sl280\tx1720 Alw21I/GWGCW'C//\par +Alw26I/GTCTCN'NNNN/'NNNNNGATCC//\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab +To search for a subset of the restriction enzymes in a file the user should reject "Search for all names" and the program will ask for the names of the enzymes wanted and extract their recognition sequences from the file. Alternatively, if a user was al +ways using the same subset, then a file containing only those enzymes could be created by editing the standard file. This file would then be selected as "Personal file" for "Input source".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +The routine also allows names and recognition sequence to be entered on the keyboard. This is selected as "Keyboard" for "Input source", and the program will prompt for names and their recognition sequences. In this way the routine can be used to search + for exact matches to any short sequence. Again IUB redundancy codes can be used.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab When back translating +from proteins it is often useful to produce a back translation using both a table of codon preferences and one using the IUB symbols. This is because the restriction enzyme search program can distinguish between definite and possible cuts in the sequence. +Those matches that the program terms "definite matches" are ones in which the specification of the recognition sequence corresponds exactly to that of the back translation. The program will also find what it terms "possible matches" which are ones that dep +end on the particular codons chosen for each amino acid. These are sites at which recognition sequences could be engineered to produce a cut in the DNA without changing the amino acid, but which are not necessarily found in the original sequence. \par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 9. Statistical and Structural Analysis of Nucleotide Sequences\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +2.\tab Methods\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.1\tab Calculating the base composition\par +2.2\tab Calculating the dinucleotide composition\par +2.3\tab Calculating the codon composition\par +2.4 \tab Creating a codon usage file\par +2.5\tab Plotting the base composition\par +2.6 \tab Searching for anomalous compositions\par +2.7\tab Search for anomalous word usage\par +2.8\tab Calculate codon constraint\par +2.9 \tab Searching for stem-loops\par +2.10\tab Searching for long range inverted repeats\par +2.11\tab Searching for long range repeats\par +2.12\tab Searching for repeated words\par +2.13\tab Searching for possible Z DNA\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Notes\par +4.\tab References\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 In this chapter we deal with performing simple statistical and structural analysis of nucleotide sequences and also describe some more unusual test +s. We cover base, dinucleotide and codon compositions, potential amino acid compositions, and the relative frequencies of each base in each position of codons. We describe how to produce plots to show regions of unusual composition and to measure the codon + bias for a gene. In addition we describe a set of functions for finding "structures" in nucleotide sequences, including short range inverted repeats or stem-loops, long range inverted repeats, long range direct repeats, and Z DNA. All the methods are cont +ained in the program NIP.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 2.\tab Methods\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.1\tab Calculating the base composition\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 \tab Select "Calculate base composition". The composition of the active region is shown.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 \page 2.2\tab Calculating the dinucleotide composition\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 \tab +Select "Calculate dinucleotide composition". The dinucleotide composition of the active region and an expected dinucleotide composition is shown. The expected composition is calculated from the base composition assuming a random order of bases in the sequ +ence. See figure 9.1.\par +\pard\plain \li1180\ri1440\sb200\sl220\box\brsp100\brdrth \f4\fs16 T C A G\par +\pard \li1180\ri1440\sl220\box\brsp100\brdrth Obs Expected Obs Expected Obs Expected Obs Expected\par +T 5.86 5.97 6.18 5.99 4.24 5.91 8.14 6.56\par +C 6.10 5.99 5.14 6.02 5.91 5.93 7.38 6.59\par +A 5.57 5.91 5.64 5.93 7.91 5.84 5.05 6.49\par +\pard \li1180\ri1440\sl220\keepn\box\brsp100\brdrth G 6.90 6.56 7.56 6.59 6.11 6.49 6.30 7.22\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa200\sl240\tx1140 \f21\fs20 Figure 9.1\tab The dinucleotide composition display\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.3\tab Calculating the codon composition\par +\pard\plain \s4\qj\sa120\sl280 \f20 This function counts codons, amino acid composition, protein molecular weights, hydrophobicity and base compos +itions. Users select the segments of the sequence to be analysed. The segments can be defined on the keyboard or from an EMBL/GenBank feature table.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Calculate codon composition".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Accept "Show observed counts". The alternative displays its codon tables so that the total for each amino acid sums to 100. This makes it easier to see any bias present in the codon usage.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Accept "Define segments using keyboard". The alternative is to use a feature table.\par +4.\tab Define "From". The start of the segment to be analysed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab +Define "To". The end of the segment to be analysed. The results will be displayed as in figure 9.2 and then the program will again ask "From". The user should define a zero value for "From" when all segments of interest have been analysed. The program w +ill then display a cummulative total for all the values it calculates.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The counts are broken down into several figures. Apart from the codon counts we see the base composition by position in codon expressed as a percentage of each bases own + frequency; base composition by position in codon expressed as a percentage of the overall base composition of the segment; base composition expected for the observed amino acid composition if there was no codon preference; percentage deviations of the ob +served amino acid composition from an average amino acid composition (1) ; the molecular weight and hydrophobicity (2) of the putative amino acid sequence.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.4 Creating a codon usage file\par +\pard\plain \s4\qj\sa120\sl280 \f20 This method writes a file of codon usage in the form of a codon tab +le (see figure 9.2). Such tables can be used by several other methods contained within the programs. If required the user can start with an existing file and add to it.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Calculate a codon table and write it to disk".\par +2.\tab Accept "Start with empty table".\par +\pard\plain \li440\ri500\sl220\pagebb\box\brsp100\brdrth \f4\fs16 Calculate base, codon and amino acid compositions\par +\pard \li440\ri500\sl220\box\brsp100\brdrth ? Show observed counts (y/n) (y) =\par + ? Define segments using keyboard (y/n) (y) =\par +\par +? From (0-8134) (0) =1\par +? To (1-8134) (8134) =1000\par +? + strand (y/n) (y) =\par + ===========================================\par + F TTT 5. S TCT 7. Y TAT 4. C TGT 2.\par + F TTC 17. S TCC 3. Y TAC 5. C TGC 3.\par + L TTA 3. S TCA 4. * TAA 3. * TGA 1.\par + L TTG 4. S TCG 3. * TAG 0. W TGG 7.\par + ===========================================\par + L CTT 3. P CCT 6. H CAT 6. R CGT 3.\par + L CTC 1. P CCC 1. H CAC 4. R CGC 2.\par + L CTA 0. P CCA 4. Q CAA 3. R CGA 1.\par + L CTG 36. P CCG 6. Q CAG 5. R CGG 4.\par + ===========================================\par + I ATT 12. T ACT 3. N AAT 6. S AGT 0.\par + I ATC 13. T ACC 5. N AAC 7. S AGC 7.\par + I ATA 1. T ACA 2. K AAA 9. R AGA 0.\par + M ATG 9. T ACG 7. K AAG 3. R AGG 1.\par + ===========================================\par + V GTT 6. A GCT 5. D GAT 7. G GGT 9.\par + V GTC 3. A GCC 6. D GAC 6. G GGC 9.\par + V GTA 7. A GCA 2. E GAA 5. G GGA 5.\par + V GTG 9. A GCG 7. E GAG 3. G GGG 3.\par + ===========================================\par + Total codons= 333.\par + T C A G\par +1 25.00 34.27 40.28 35.94\par +2 45.42 28.63 36.02 22.27\par +3 29.58 37.10 23.70 41.80\par + ----- ----- ----- -----\par += 100% 100% 100% 100%\par +1 21.32 25.53 25.53 27.63 = 100%\par +2 38.74 21.32 22.82 17.12 = 100%\par +3 25.23 27.63 15.02 32.13 = 100%\par +% 28.43 24.82 21.12 25.63 Observed, overall totals\par +% 29.65 23.25 23.95 23.15 Expected, even codons per acid\par + A C D E F G H I K L\par + 20. 5. 13. 8. 22. 26. 10. 26. 12. 47.\par +O-E % -27. -11. -25. -61. 71. 10. 38. 52. -36. 59.\par + M N P Q R S T V W Y\par + 9. 13. 17. 8. 11. 24. 17. 25. 7. 9.\par +O-E % 14. -10. 1. -39. -41. 6. -11. 15. 64. -15.\par +\pard \li440\ri500\sl220\keepn\box\brsp100\brdrth Total acids= 329. Molecular weight= 36493. Hydrophobicity= 64.7\par +\pard\plain \s8\qj\fi-1140\li1140\sb80\sa280\sl240\tx1140 \f21\fs20 Figure 9.2\tab A worked example of calculating codon, base and amino acid compositions.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 3.\tab Accept "Show observed counts". The alternative is to have the counts for each amino acid type sum to 100.\par +4.\tab Accept "Define segments using keyboard". The alternative is to use an EMBL/GenBank feature table.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Define "From". The start of the segment to count over.\par +6.\tab Define "To". The end of the segment.\par +7.\tab Accept "+ strand". Alternatively the minus strand.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \tab The table will appear on the screen and the program will cycle round to step 5. When all segments have been defined a zero v +alue for "From" will instruct the program to display on the screen a table which is the sum of all the individual tables.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 8.\tab Define "Name for codon table file". Give the name of the file in which to save the final table. \par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.5\tab Plotting the base composition\par +\pard\plain \s4\qj\sa120\sl280 \f20 This function plots the base composition for each "window length" of the sequence. The frequency of any combinations of bases can be plotted.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Plot base composition".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Select which combination of bases to plot. The default is A+T, but any single base or combination of bases can be used.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +Select "Odd window length". This is the size of window over which each count is made, it is "odd" so that the plotted point exactly corresponds to the centre of each window. The count is made over the window and then the window is moved on by 1 base, an +d the count repeated.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Plot interval". Especially when using long windows it is unnecessary to plot the results for every point along the sequence. A plot interval of 5 will mean the value for every fif +th point will be plotted. 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This is an A+T plot for bacteriophage Lambda and shows that one half is A+T rich and the other G+C rich.\par +\pard\plain \s6\sb240\sa100\sl280\tx560\tx860 \b\f20 2.6\tab Searching for anomalous compositions\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This "search" is performed by comparing a standard composition against each segment of the sequence and plotting the difference. The difference between the observed and expected composition at each point is expressed as the chi-square value. + Any one of the base, dinucleotide or trinucleotide compositions can be used as the standard. No expected level of divergence is used so the program always displays the results so that the plots fill the alloted space on the screen. At the end the observed + range is displayed.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Plot dinucleotide composition differences as chi squared". Alternatively select base or trinucleotides.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Define "Start". Define the position of the first base to be used in the standard.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Define "End". Define last base of the standard. The default standard region is the whole sequence.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Odd window length". \par +5.\tab Define "Plot interval".\par +\pard\plain \s4\qj\sa120\sl280 \f20 The plot will appear as in figure 9.4\par +\pard\plain \sb200\sl220\keepn \f4\fs16 {{\pict\macpict\picw448\pich119 +06f6ffffffff007601bf1101a0008201000affffffff007601bf0900000000000000003100000000007501be98002400000000004e012000000000004e011f00000000007501be000102dd0006007fdfff00fc0a0040fc000002e50000040a0040fc000002e50000040a0040fc000003e50000040a0040fc000003e5000004 +0a0040fc000003e50000040b0040fc00010380e60000040b0040fc00010280e60000040b0040fc00010280e60000040b0040fc00010240e60000040b0040fc00010240e60000040d0040fc0003027ffff8e80000040d0040fc000302000008e80000040d0040fc000302000008e80000040d0040fc000302000008e8000004 +0d0040fc000302000008e80000040d0040fc000302000008e80000040d0040fc000304000008e80000040d0040fc000304000004e80000040d0040fc000304000004e80000040d0040fc000304000004e80000040d0040fc000304000004e80000040d0040fc000304000004e80000040d0040fc000304000004e80000040e +0040fc000304000004e9000102040e0040fc000304000004e9000102040e0040fc000304000004e9000102040e0040fc000304000004e9000102040e0040fc000304000004e9000102040e0040fc000304000004e9000102040e0040fc000304000004e9000102040e0040fc000304000004e9000102040e0040fc00030400 +0004e9000102040e0040fc000304000004e9000102040e0040fc000304000004e9000102040e0040fc000304000004e9000102040e0040fc000304000002e9000102040f0040fc000304000002ea00028002040f0040fc000304000002ea00028002040f0040fc000304000002ea0002a006040f0040fc000304000002ea00 +02a00604130040fc000304000002f6000080f60002a00604130040fc000304000002f6000080f60002a00604130040fc000304000002f6000080f60002a00544140040fc000308000002f700010180f60002a00564150040fc000308000002f700010180f70003017005641a0040fc000308000002fd000003fc00010180f8 +000420015005441a0040fc000308000002fd000003fc00010140f8000420015005441a0040fc000308000002fd000005fc00010240f8000420015005441e0040fe000620000800000202fe000005fd0002400240f8000430015005841e0040fe000620000800000202fe000005fd0002400240f8000430015005841f0040fe +000620000800000202fe00010480fe0002400240f8000450015005841f0040fe0011200008000001060002020480000001c00240f8000450015005841f0040fe0011300008000001060002020480000001c00240f800045001100584230040fe0011300008000001050002020480000001c00440fd000018fd000450011005 +84230040fe0011300008000001050006060480020001a00440fd000014fd00044801080584230040fe0011300008000001050006060480020001200440fd000017fd0004c801080984241540018000500008000001090006060880060001200440fd0009110008800088020809842415400160005000080000010900050608 +80050002200420fd0009110008800108020809042523400120005000080000010900050608800500022004200180080021000880010802080904252340012000500008000001110005050880050002200420028008002080088001080208090425234002200050001000000111000905888005040221842002800810208018 +a001080208090425234002200050001000000111000905888009040221882002800810608018a00108020a1104250640022000500010fe001990800909884008870211882004401410608015600108020a1004250640021000500010fe001990800989884008950211882004401430808075600105020a1004250640021000 +500010fe001990800889484008f90212882004401428808045500105020a1004250640021000500010fe0019a080088948400809020a88200440142880804550010502061004250640021800900010fe0019a080108948400808840a48100440142880804550010704051004250640041800880050fe0019a08010b9504090 +08840a4810044012ac8040455101070405a004250640041802880050fe0019a080106050409000840a5010042022c50040835101068405a00425064004084b0800d0fe0019a08010605048900094065010082021430040821281028405a00425064004064d0dffa0fe0019a04010403048d000b40450104831e1030044800e +8900840560042506780406b4020020fe00196040204020555000b804700c48124003004480088900840560042506480805b4020020fe000d40402040203560006804100ca80cfe00087a80085600880040042203444805b0fb000d405fa0000033600048000003b80cfe00080a80005600d000400406007fdfff00fc02dd00 +a00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa400\sl240\tx1140 \f21\fs20 Figure 9.4\tab An anomalous composition plot. This shows an immunoglobulin switch region and the plateau corresponds to a segment composed entirely of A and G bases.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.7\tab Search for anomalous word usage\par +\pard\plain \s4\qj\sa120\sl280 \f20 This function is designed to examine the abundances of short words in a nucleotide sequence to see if particular ones are either under or over repre +sented (3). It compares the observed and expected frequencies and plots them for each segment of the sequence. There has been some work on the relative abundances of CG dinucleotides in eukaryotic sequences (e.g. reference 4) and this routine can be used t +o examine such biases or any others that might be of interest.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Plot observed-expected word usage".\par +2.\tab Define "String". That is the word to search for. The default is CG.\par +3.\tab Define "Odd window length".\par +4.\tab Define "Plot interval".\par +5.\tab Define "Maximum plot value". Define the maximum expected value for the plot.\par +6.\tab Define "Minimum plot value".\par +\pard\plain \s4\qj\sa120\sl280 \f20 The plot will appear as in figure 9.5.\par +\pard\plain \ri-60\sb200\sl220\keepn \f4\fs16 {\plain \fs16 {\pict\macpict\picw453\pich122 +0800ffffffff007901c41101a00082a0008c01000affffffff007901c4070000000022000100010000a000a0a100a400020de801000a000000000000000007000100012200770001008a23000021000101c32300002300762300002100770001230000a000a301000affffffff007901c423008a21000101c3230076210077 +0001a000a120003b0001003b01c322003b00011a082300022302002300fe2302022300fe2301fe2300022300fe2302022300fe2301022300002302002300002300fe2302022300002301022300002302fc2300042300fe2301fe2300ff2302012300022301002300fd2300002302002300002302002300002301fe23000223 +00002302fe2300022301fd2300032300002302fd2300002302fe2300002300002301ff2300002302fe2300002301002300002300022302012300022301002300012302002300002300022302002300002301002300002302002300002301002300012300022302fe2300022302fe2300002301002300022300002302fe2300 +022301002300002300fe2302022300002301002300002300022302002300012302002300022301002300002300012302002300022301fe2300002302022300002300002302002300002301002300032302002300002300ff2301012300002302ff2300002301002300002300012302ff2300002302012300002301022300fe +2300002302022300fe2300002301fd2300002302022300002302012300002300ff2301fc2300002302002300ff2301012300002300002302022300fd2302002300fe2301ff2300032302fe2300002300022301002300012302002300002301ff2300002300002302002300012302ff2300012301ff2300fe23000223020123 +00002301002300022300002302fe2300022302fd2300fe2300002301022300002302002300002301002300fe2300022302002300012301002300022302fe2300002300002302002300002301002300022302002300002300002301002300002302002300002302002300032300fb2301002300022302002300022301012300 +002300022302002300002300002301012300022302012300ff2302002300002300fe2301002300002302002300002301002300022300002302002300fe2302022300012301ff2300002302fe2300ff2300002301fe2300022302fe2300002301ff2300002300002302002300fe230200230000230100230000230002230201 +2300ff2300012301022300002302fe2300002302002300002300002301022300fe2302fd2300002301002300002300022302002300fe2301022300012302022300002300fe2302022300002301002300fe2302ff2300002300012301ff2300042302002300002302002300ff2300012301fd2300002302022300002301fe23 +00002300002302002300002300002302002300002301002300002302002300ff2300002301fe2300022302fe2300022301fe2300fe2300002302002300022302fe2300022301fe2300ff2302fe2300022300002301002300002302002300012302022300002300fe2301022300fe2302fd2300022301002300fe2300002302 +022300002300002301012300ff2302fe2300032302002300ff2300002301012300ff2302fe2300002301002300002300002302ff2300012302002300ff2301002300012300002302fd2300022301002300fe2302032300002300002301022300fe2302022300012302ff2300002300012301ff230000230200230000230000 +2301002300012302ff2300002300012302002300ff2301002300002302012300002300022301002300002302002300002301022300002302012300002300002302ff2300012301022300fe23020023000023000023010023000223020023000123020223000123000023010223000023020023000023010023000023000223 +02012300002300022301002300002302002300012302002300002300ff2301002300002302002300002301012300ff2300002302002300002302012300002301002300002300002302002300002301ff2300002302002300012300002301ff2300fe2302032300ff2302002300002300002301002300002302002300fe2300 +002301022300012302002300002300ff2302002300002301002300002302002300002300012301002300002302002300002302022300fd2301fe2300022300012302ff2300012301002300ff2302002300012300fd2301022300fe2302002300002302022300002300002301fe2300002302fd2300fe2300002301022300fe +2302002300002300022302002300032301ff2300002302002300012300ff2301012300002302032300002301022300022300fb23020123000023020023000023010023000223000023020023000023010223000023020023000023000123020023000023010223000023020023000023000023010023000023000023020023 +00012301002300002302ff2300012300002302022300002301fe2300022302002300012300002301042300002302002300012302002300002301002300002300002302002300002301002300ff2302fe2300fe2300002301ff2300002302032300fe2302022300002300fe2301ff2300002302002300002300fe2301002300 +002302002300fd2300ff2302002300012301fb2300002302002300fd2300ff2301fe2300032302ff2300002301fe2300fd2300012302ff2300012302fd2300ff2301002300002300fe2302ff2300fe2301002300002302002300fe2300002302002300002301002300ff2302002300fe2300ff2301fe2300fd2300002302fe +2300ff2302fe230000a0008da00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa400\sl240\tx1140 \f21\fs20 Figure 9.5\tab +A plot of anomalous word usage. This shows a plot of CG usage for the Human CMV immediate-early region. The frequency of CG is much lower than would be expected from the composition.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 \page 2.8\tab Calculate codon constraint\par +\pard\plain \s4\qj\sa120\sl280 \f20 This method measures the level of constraint imposed on a sequence by coding for a protein. The codon constraint is the difference between the observe +d codon improbability and the mean improbability for a sequence of the same composition. That is it is a measure of the codon bias and the program performs the calculation over windows of length 99 codons. See reference 5. The user can select segments to a +nalyse either by defining them on the keyboard or by using an EMBL/GenBank feature table. The result for each selected segment, which is simply a single number, is displayed.\par +\pard\plain \s7\qj\fi-560\li560\sa80\sl280\tx560 \f20 1.\tab Select "Calculate codon constraint".\par +2.\tab Accept "Define segments using keyboard".\par +3.\tab Define "From". The start of the segment.\par +4.\tab Define "To". The end of the segment.\par +5.\tab Accept "+ strand".\par +\pard\plain \s4\qj\sa120\sl280 \f20 The result will be displayed, and the program will ask for the next segment to be defined. \par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.9\tab Searching for stem-loop structures\par +\pard\plain \s4\qj\sa120\sl280 \f20 This routine finds simple putative stem-loop structures having a minimum number of base pairs in their stems. Results can be listed or plotted.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Search for hairpin loops".\par +2.\tab Define "Minimum loop size".\par +3.\tab Define "Maximum loop size".\par +4.\tab Define "Minimum number of base pairs"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Reject "Plot results". The alternative writes out the stem-loops as shown in figure 9.6. The plotted output marks the position of each stem, the height of the mark showing the length of the stem.\par +\pard\plain \li3480\ri3940\sb200\sl220\box\brsp100\brdrth \f4\fs16 g\par +\pard \li3480\ri3940\sl220\box\brsp100\brdrth g.t\par + t.g\par + c-g\par + a-t\par + t.g\par + t.g\par + g-c\par + t.g\par + g.t\par + g.t\par + t.g\par + t.g\par + g-c\par + t.g\par +tggcga gttttaa\par +\pard \li3480\ri3940\sl220\keepn\box\brsp100\brdrth 843\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa400\sl240\tx1140 \f21\fs20 Figure 9.6\tab A typical textual display from the routine for finding simple hairpin loops.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 \page 2.10\tab Searching for long range inverted repeats\par +\pard\plain \s4\qj\sa120\sl280 \f20 This method finds inverted repeats. It allows for no mismatches, insertions or deletions within the matching segments.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Find long range inverted repeats".\par +2.\tab Accept "Plot results". The alternatve lists out all the matching segments.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Define "Start". The beginning of the region to analyse. In general the whole sequence will be analysed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "End".\par +5.\tab Define "Minimum inverted repeat". The length of the minimum match.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The results will now be plotted in an unusual way as shown in figure 9.7 in which the positions of matching segments are joined by rectangular lines.\par +\pard\plain \li100\sb200\sl220\keepn\box\brsp20\brdrth \f4\fs16 {{\pict\macpict\picw445\pich118 +0448ffffffff007501bc1101a0008201000affffffff007501bc0900000000000000003100000000007401bb98001e00000000003d00f000000000003d00ec00000000007401bb000102e3000701001fe6ff00c007010018e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c0070100 +18e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c007010018e60000c00a00 +7ff1ff00c0f60000c00b014018f2000040f60000c00b014018f2000040f60000c00b014018f2000040f60000c00b014018f2000040f60000c00b014018f2000040f60000c00b014018f2000040f60000c00b014018f2000040f60000c00b014018f2000040f60000c00e007ff5ff00e0fe000040f60000c00f017818fb0000 +01f4ff00f0fb0000c019017818fb000501c1800000e0fe000040fe00017030fb0000c019017818fb000501c1800000e0fe000040fe00017030fb0000c019017818fb000501c1800000e0fe000040fe00017030fb0000c01502781807f7ff00e0fe000040fe00017030fb0000c01a02781804fc000501c1800000e0fe000040 +fe00017030fb0000c01a02781804fc000501c1800000e0fe000040fe00017030fb0000c01a02781804fc000501c1800000e0fe000040fe00017030fb0000c01a02781804fc000501c1800000e0fe000040fe00017030fb0000c01a02781804fc000501c1800000e0fe000040fe00017030fb0000c01a02781804fc000501c1 +800000e0fe000040fe00017030fb0000c01a02781804fc000501c1800000e0fe000040fe00017030fb0000c01a02781804fc000501c1800000e0fe000040fe00017030fb0000c01102781804fc000001f5ff01f030fb0000c01a02781804fc000e01c1800000f0006000400008007030fb0000c01a02781804fc000e01c180 +0000f0006000400008007030fb0000c01a02781804fc000e01c1800000f0006000400008007030fb0000c01a02781804fc000e01c1800000f0006000400008007030fb0000c01a02781804fc000e01c1800000f0006000400008007030fb0000c01a02781804fc000e01c1800000f0006000400008007030fb0000c01c1678 +1804000007ffffe1c1800000f0006000400008007030fb0000c01c167ffffc000007ffffe1c1800000f0006000400008007030fb0000c01c16781804000007ffffe1c1800000f0006000400008007030fb0000c01c16781804000007ffffe1c1800000f0006000400008007030fb0000c01c1678180407fe07ffffe1c18000 +00f0006000400008007030fb0000c01c1678180407fe07ffffe1c1800000f0006000400008007030fb0000c01c1678180407fe07ffffe1c1800000f0006000400008007030fb0000c002e300a00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl20\tx1140 \f21\fs20 Figure 9.7\tab +A plot of direct or inverted repeats. Each matching segment is joined by a rectangular line. Here we show the direct repeats of at least 25 bases in a mouse immunoglobulin switch region.\par +\pard\plain \s6\sb120\sa40\sl280\tx560\tx860 \b\f20 2.11\tab Searching for long range repeats\par +\pard\plain \s4\qj\sa120\sl260 \f20 This method finds direct repeats. It allows for no mismatches, insertions or deletions within the matching segments.\par +\pard\plain \s7\qj\fi-560\li560\sa80\sl260\tx560 \f20 1.\tab Select "Find long range repeats".\par +2.\tab Accept "Plot results". The alternatve lists out all the matching segments.\par +\pard \s7\qj\fi-560\li560\sa80\sl260\tx560 3.\tab Define "Start". The beginning of the region to analyse. In general the whole sequence will be analysed.\par +\pard \s7\qj\fi-560\li560\sa80\sl260\tx560 4.\tab Define "End".\par +5.\tab Define "Minimum repeat". The length of the minimum match.\par +\pard \s7\qj\fi-560\li560\sa120\sl260\tx560 The results will now be plotted in an unusual way as shown in figure 9.7 in which the positions of matching segments are joined by rectangular lines.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.12\tab Searching for repeated words\par +\pard\plain \s7\qj\sa120\sl260\tx540 \f20 \tab This function can be used to examine the frequencies of repeated words within a sequence. It finds all words that occ +ur more than once. A "word" is a particular sequence of bases so we are dealing only with exact repeats. The user selects a minimum word length and the program finds all words of that length that occur more than once. Then it "follows" each repeated word u +ntil it becomes unique. For each word length it can report the number of different repeated words, the number of occurrences of each word, and their actual sequences and positions.\par +\pard \s7\qj\fi-560\li560\sa120\sl260\tx560 1.\tab Select "Examine repeats".\par +\pard \s7\qj\fi-560\li560\sa120\sl260\tx560 2.\tab Define "Minimum word length". The maximum expected and observed word lengths are displayed.\par +3.\tab Define "Minimum word length for display of repeated word frequencies". The number of different repeated words of each length is listed.\par +\pard \s7\qj\fi-560\li560\sa120\sl260\tx560 4.\tab Define "Minimum frequency for display of repeated words". \par +\pard \s7\qj\fi-560\li560\sa120\sl260\tx560 5.\tab Define "Minimum word length for display of repeated words". All words occurring this number of times and of this given word length will be displayed.\par +\pard \s7\qj\fi-560\li560\sa120\sl260\tx560 \par +\pard\plain \sl220\box\brsp100\brdrth \f4\fs16 {\f22\fs18 Expected length of longest repeat 12\par +}\pard \sl220\box\brsp100\brdrth {\f22\fs18 ? Minumim word length (1-6) (6) = \par +Working\par + Memory used in bytes 75164. Length of longest repeat 13\par + ? Show repeat frequencies for words of at least length (6-13) (13) = 10\par + For length 10 the number of different repeated words is 86\par + For length 11 the number of different repeated words is 21\par + For length 12 the number of different repeated words is 5\par + For length 13 the number of different repeated words is 2\par + ? Show repeats for words of length (6-13) (13) = 10\par + ? Show repeats for words occuring with frequency (2-9999) (2) = 3\par + aaggcatcat\par + occurs at 276\par + occurs at 969\par + occurs at 6938\par + gtctggcggc\par + occurs at 1891\par + occurs at 4714\par + occurs at 7250\par + ? Show repeats for words of length (6-13) (13) = 12\par + ? Show repeats for words occuring with frequency (2-9999) (2) = \par + gttactggtggt\par + occurs at 641\par + occurs at 851\par + aaaggcatcatg\par + occurs at 968\par + occurs at 6937\par + aaggcatcatgg\par + occurs at 969\par + occurs at 6938\par + ttactggtggtg\par + occurs at 642\par + occurs at 852\par + ctgctgggccgt\par + occurs at 3477\par + occurs at 6424\par +}\pard \sl220\box\brsp100\brdrth {\f22\fs18 ? Show repeats for words of length (6-13) (13) =!\par +}\pard \sl220 {\f22\fs18 \par +}{\f22\fs20 Figure 9.8 Typical output from "Examine repeats".\par +}\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 \par +2.13\tab Searching for possible Z DNA\par +\pard\plain \s4\qj\sa60\sl260 \f20 +The program contains three algorithms for searching for sequences with the potential for forming Z DNA. In varying ways they look for segments of alternating purines and pyrimidines and they all plot their results. A typical result is shown in figure 9.9. +\par +\pard\plain \sl220\keepn \f4\fs16 {{\pict\macpict\picw447\pich119 +0512ffffffff007601be1101a0008201000affffffff007601be0900000000000000003100000000007501bd98002400000000004e012000000000004e011f00000000007501bd000102dd0006007fdfff00fc060040df000004060040df000004060040df000004060040df000004060040df000004060040df0000040600 +40df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040 +df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df000004060040df0000040e0040f4000001fb000080f40000040e0040f4000001fb000080f40000040e0040f4000001fb000080f40000040e0040f4000001fb000080f40000040e0040f4000001 +fb000080f40000040e0040f4000001fb000080f40000040e0040f4000001fb000080f40000041202400040f6000001fb000080f600022000041202400040f6000001fb000080f600022000041202400040f6000001fb000080f600022000041202400040f6000001fb000080f600022000041202400040f6000001fb000080 +f600022000041202400040f6000001fb000080f600022000041202400040f6000001fb000080f600022000041702400040f6000001fb000380000004fb000440002000041702400040f6000001fb000380000004fb000440002000041702400040f6000001fb000380000004fb000440002000041702400040f6000001fb00 +0380000004fb000440002000041802400040f600010180fc0003c0000004fb000440002000041802400040f600010180fc0003c0000004fb000440002000041802400040f600010180fc0003c0000004fb0004400020000421044000400020fc0005020002000181fc0006c0004004000440fe000440003084142104400040 +0020fc0005020002000181fc0006c0004004000440fe0004400030841421044000400020fc0005020002000181fc0006c0004004000440fe0004400030841421044000400020fc0005020002000181fc0006c0004004000440fe0004400030841422044000c00030fc0005020003000281fd0007014000c006000440fe0004 +600051843c22044000c00030fc0005020003000281fd0007014000c006000440fe0004600051843c22044000c00030fc0005020003000281fd0007014000c006000440fe0004600051843c23044000c01430fc001903004300028181020042014000c0060146600040006404d3563c23044000c01430fc0019030043000281 +81020042014000c0060146600060006404d3563c23044000c01c30fc001903004300028181020042014000c00601e6600060006406d3563c23044000c01c30fc001903004300028181020042014000c00601e6600050006406d3563c23044000c01e28fc0019030062800282818600a3014000c00601e6a00088006406d55e +3c23044000c01628fc0019030062800282818600a3014000c0060156a00088006405d55e3c23044000c01628fc0019030062800282818600a3014000c0060156a00084006405d55e3c20045ffffff7effaff03fefffefefeff02bfff7ffdff095fbfff87fffffddd7ffc060050df000004060060df000004060060df000004 +060060df000004060040df00000406007fdfff00fc02dd00a00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 9.9\tab A plot of predictions for potential Z DNA containing some high peaks produced by regions of alternating purines and pyrimidines.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 3.\tab Notes\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Whenever the program reads a sequence file it always displays the base composition to provide the user with a check on the correctness of the file.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab +The search for anomalous words function operates in the following way. Users select a "word" - say CG and a window length. The program examines each successive window length along the sequence, with each window overlapping the previous one by windowleng +th-1 bases. For each window position the program calculates the base composition and the number of +occurrences of the chosen word. From the base composition it calculates an expected number of occurrences of the chosen word by simply multiplying the relevent frequencies and assuming random ordering. It plots observed - expected hence showing regions tha +t are enriched or depleted in the chosen word.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +The codon constraint calculation offers a measure of the codon bias that is independent of any set tables of expected codons. Although some users may find the underlying mathematics difficult to understand +the values obtained provide an interesting measure. It was shown (5) for a set of {\i E. coli} genes that their values of codon constraint correlated with their levels of expression. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab The algorithm for finding possible stem loops counts A-T, G-C and G-T pairs as matching but will only find stems with no mismatches or loopouts.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab The long range inverted and direct repeat routines are fast but only find exact matches. More flexible and exhaustive methods are described in the chapter on sequence comparisons.\par +6.\tab It is also possible to use the pattern searching routines to define and search for inverted and direct repeats. They are particularly useful for finding specific structures - for example tRNA folds.\par +\pard \s7\qj\fi-560\li560\sa120\sl260\tx560 7.\tab +It is possible that the "Examine repeats" algorithm may run out of memory, particularly if a short minimum word length is chosen or the sequence is very long or very repetitive. If this occurs the maximum word length reported may not be the longest in t +he sequence\: the memory will have been consumed before it was found.\par +\pard\plain \s5\sb320\sa60\sl320\tx560 \b\f20\fs28 \page 4.\tab References\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab McCaldon,P. and Argos,P. 1988 Oligopeptide biases in protein sequences and their use in predicting protein coding regions in nucleotide sequences. {\i Proteins} {\b 4}, 99-122.\par +2.\tab Sweet,R.M. and Eisenberg,D. 1983. Correlation of sequence hydrophobicity measures similarity in three-dimensional protein structure. {\i J. Mol. Biol}. {\b 171}\:479-488.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Honess,R.W., Gompels,U.A., Barrell,B.G., Craxton,M., Cameron,K.R., Staden,R., Chang,Y.-N and Hayward,G.S. 1989 Deviations from expected frequencies +of CpG dinucleotides in herpesvirus DNAs may be diagnostic of differences in the states of their latent genomes. {\i J. Gen. Virol}, {\b 70}, 837-855.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Bird,A.P. 1980 DNA methylation and the frequency of CpG in animal DNA. {\i Nucl. Acids Res}. {\b 8}, 1499-1504.\par +5.\tab McLachlan, A.D., Staden, R., and Boswell, D.R. 1984. A method for measuring the non-random bias of a codon usage table. {\i Nucl. Acids Res}. {\b 12}\:9567-9575.\par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 10. Translating and Listing Nucleic Acid Sequences\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +2.\tab Methods\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.1\tab Listing the sequence with all six reading frames translated\par +2.2\tab Listing the sequence with its open reading frames translated\par +2.3\tab Listing the sequence with defined segments translated\par +2.4\tab Listing the sequence with translated segments defined from a feature table\par +2.5\tab Producing a file of protein sequences for all open reading frames.\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.6\tab Producing a file of protein sequences for segments defined from a feature table\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Notes\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 In this chapter we deal with producing simple listings from nucleotide seque +nces. All functions are contained in the program NIP. We can list the sequence alone, in single or doubled stranded format or with translations to protein. The translations can be of all six phases, all open reading frames, or of specified segments. The p +ositions of these segments can be defined on the keyboard or read from a EMBL/GenBank feature table. Translations can use the one letter or three letter codes. In addition we can produce files containing only the protein translations, and which are suitabl +e for processing by other programs. Again the positions of the translated segments can be defined on the keyboard, read from a feature table, or be all open reading frames. For the user, producing all these results is very simple, so we only give examples +of "methods" and show what the results look like. All outputs that list the sequence can be produced from the menu option named "Translate and list".\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 2.\tab Methods\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.1\tab Listing the sequence with all six reading frames translated\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Translate and list".\par +2.\tab Accept "Show translation".\par +3.\tab Select "The segments to translate will be "All six frames"".\par +4.\tab Accept "Use 1 letter codes".\par +5.\tab Define "Start". Where to list from.\par +6.\tab Define "End". Where to list to.\par +7.\tab Define "Line length". The number of characters in each line of output.\par +8.\tab Reject "Number ends of lines". This alternative writes the positions underneath each line.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The listing will then appear. Given the choices taken it will look the same as figure 10.1.\par +\pard\plain \li1240\ri1280\sb200\sl220\box\brsp100\brdrth \f4\fs16 Q D Y I G H H L N N L Q L D L R T F S L\par +\pard \li1240\ri1280\sl220\box\brsp100\brdrth R I T * D T T * I T F S W T C V H S R W\par + G L H R T P P E * P S A G P A Y I L A\par +caggattacataggacaccacctgaataaccttcagctggacctgcgtacattctcgctg\par + 1010 1020 1030 1040 1050 1060\par +gtcctaatgtatcctgtggtggacttattggaagtcgacctggacgcatgtaagagcgac\par + L I V Y S V V Q I V K L Q V Q T C E R Q\par + P N C L V G G S Y G E A P G A Y M R A P\par + S * M P C W R F L R * S S R R V N E S\par +\par + V D P Q N P P A T F W T I N I D S M F F\par + W I H K T P Q P P S G Q S I L T P C S S\par +G G S T K P P S H L L D N Q Y * L H V L\par +gtggatccacaaaaccccccagccaccttctggacaatcaatattgactccatgttcttc\par + 1070 1080 1090 1100 1110 1120\par +cacctaggtgttttggggggtcggtggaagacctgttagttataactgaggtacaagaag\par + H I W L V G W G G E P C D I N V G H E E\par + P D V F G G L W R R S L * Y Q S W T R R\par +T S G C F G G A V K Q V I L I S E M N K\par +\par + S V V L G L L F L V L F R S V A K K A T\par + R W C W V C C S W F Y S V A * P K R R P\par +L G G A G S V V P G F I P * R S Q K G D\par +tcggtggtgctgggtctgttgttcctggttttattccgtagcgtagccaaaaaggcgacc\par + 1130 1140 1150 1160 1170 1180\par +agccaccacgacccagacaacaaggaccaaaataaggcatcgcatcggtttttccgctgg\par + R H H Q T Q Q E Q N * E T A Y G F L R G\par + P P A P D T T G P K I G Y R L W F P S W\par +E T T S P R N N R T K N R L T A L F A V\par +\par + S G V P G K F Q T A I E L V I G F V N G\par + A V C Q V S F R P R L S W * S A L L M V\par +Q R C A R * V S D R D * A G D R L C * W\par +agcggtgtgccaggtaagtttcagaccgcgattgagctggtgatcggctttgttaatggt\par + 1190 1200 1210 1220 1230 1240\par +tcgccacacggtccattcaaagtctggcgctaactcgaccactagccgaaacaattacca\par + A T H W T L K L G R N L Q H D A K N I T\par + R H A L Y T E S R S Q A P S R S Q * H Y\par +\pard \li1240\ri1280\sl220\keepn\box\brsp100\brdrth L P T G P L N * V A I S S T I P K T L P\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa400\sl240\tx1140 \f21\fs20 Figure 10.1\tab A six phase translation using the 1 letter codes\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.2\tab Listing the sequence with its open reading frames translated\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Translate and list".\par +2.\tab Accept "Show translation".\par +3.\tab Select "The segments to translate will be "Open reading frames"".\par +4.\tab Define "Minimum open frame in amino acids".\par +5.\tab Accept "Use 1 letter codes".\par +6.\tab Define "Start". Where to list from.\par +7.\tab Define "End". Where to list to.\par +8.\tab Define "Line length". The number of characters in each line of output.\par +9.\tab Select "Both strands"\par +10.\tab Accept "Number ends of lines".\par +\pard\plain \s4\qj\sa120\sl280 \f20 A typical result is shown in figure 10.2.\par +\pard\plain \li720\ri680\sb200\sl220\box\brsp100\brdrth \tx7780 \f4\fs16 Q D Y I G H H L N N L Q L D L R T F S L\par +\pard \li720\ri680\sl220\box\brsp100\brdrth \tx7780 caggattacataggacaccacctgaataaccttcagctggacctgcgtacattctcgctg\tab 1060\par + . \: . \: . \: . \: . \: . \:\par +gtcctaatgtatcctgtggtggacttattggaagtcgacctggacgcatgtaagagcgac\par + L I V Y S V V Q I V K L Q V Q T C E R Q\par + * S S R R V N E S\par +\par + V D P Q N P P A T F W T I N I D S M F F\par +gtggatccacaaaaccccccagccaccttctggacaatcaatattgactccatgttcttc\tab 1120\par + . \: . \: . \: . \: . \: . \:\par +cacctaggtgttttggggggtcggtggaagacctgttagttataactgaggtacaagaag\par + H I W L V G W G G E P C D I N V G H E E\par +T S G C F G G A V K Q V I L I S E M N K\par +\par + S V V L G L L F L V L F R S V A K K A T\par +tcggtggtgctgggtctgttgttcctggttttattccgtagcgtagccaaaaaggcgacc\tab 1180\par + . \: . \: . \: . \: . \: . \:\par +agccaccacgacccagacaacaaggaccaaaataaggcatcgcatcggtttttccgctgg\par + R H H Q T Q Q E Q N * E T A Y G F L R G\par +E T T S P R N N R T K N R L T A L F A V\par +\par + S G V P G K F Q T A I E L V I G F V N G\par +agcggtgtgccaggtaagtttcagaccgcgattgagctggtgatcggctttgttaatggt\tab 1240\par + . \: . \: . \: . \: . \: . \:\par +tcgccacacggtccattcaaagtctggcgctaactcgaccactagccgaaacaattacca\par + A T H W T L K L G R N L Q H D A K N I T\par +L P T G P L N * V A I S S T I P K T L P\par +\par + S V K D M Y H G K S K L I A P L A L T I\par +agcgtgaaagacatgtaccatggcaaaagcaagctgattgctccgctggccctgacgatc\tab 1300\par + . \: . \: . \: . \: . \: . \:\par +tcgcactttctgtacatggtaccgttttcgttcgactaacgaggcgaccgggactgctag\par + A H F V H V M A F A L Q N S R Q G Q R D\par +\pard \li720\ri680\sl220\keepn\box\brsp100\brdrth \tx7780 L T F S M Y W P L L L S I A G S A R V I\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa180\sl240\tx1140 \f21\fs20 Figure 10.2\tab A listing showing the translation of open reading frames from both strands of a sequence from position 1001 to 1300\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.3\tab Listing the sequence with defined segments translated\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Translate and list".\par +2.\tab Accept "Show translation".\par +3.\tab Select "The segments to translate will be "Typed on the keyboard"".\par +4.\tab Accept "Use 1 letter codes".\par +5.\tab Define "Start". Where to list from.\par +6.\tab Define "End". Where to list to.\par +7.\tab Define "Line length". The number of characters in each line of output.\par +8.\tab Select "Both strands".\par +9.\tab Accept "Number ends of lines".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 10.\tab Define "Translate from". Define the start of the next segment to translate - say the next exon.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 11.\tab Define "Translate to". Define the end of the next segment to translate.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 12.\tab Select "Strand". As both strands have been selected above the program will allow either to be translated for each defined segment.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The program will now cycle around through steps 10, 11 and 12 until a zero value is defined for "Translate from". At which point the listing will appear. Given the choices made it will look the same as figure 10.2. +\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.4\tab Listing the sequence with translated segments defined from a feature table\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Translate and list".\par +2.\tab Accept "Show translation".\par +3.\tab Select "The segments to translate will be "Read from a feature table"".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Feature table file name". Type the name of the file containing the appropriate feature table in EMBL/GenBank format.\par +5.\tab Define "Operator". This defines which feature table operators should be employed when selecting the segments to translate.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Accept "Use 1 letter codes"\par +7.\tab Define "Start". Where to list from.\par +8.\tab Define "End". Where to list to.\par +9.\tab Define "Line length". The number of characters in each line of output.\par +10.\tab Select "Both strands"\par +11.\tab Accept "Number ends of lines".\par +\pard\plain \s4\qj\sa120\sl280 \f20 The program will now read the feature table file and translate the segments defined using the selected operator(s) and the listing will appear as in figure 10.2.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.5\tab Producing a file of protein sequences for all open reading frames.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Translate and write protein sequences to disk".\par +2.\tab Reject "Translate selected regions". The alternative is "Open reading frames".\par +3.\tab Define "Minimum open frame in amino acids".\par +4.\tab Select "Both strands".\par +5.\tab Define "File name for translation".\par +\pard\plain \s4\qj\sa120\sl280 \f20 +A typical results file is shown in figure 10.3. It shows that the file is written in FASTA format (i.e. an entry name line starting with a > symbol (here the first entry name is 188, the start of the DNA segment), followed by a title (here in EMBL feature +table format giving the start and end of the DNA that produced the protein), followed by the sequence terminated by an *.\par +\pard \s4\qj\sa120\sl280 \par +\pard\plain \sl220 \f4\fs16 {\f22\fs18 \par +}\pard \li1260\ri1360\sl220\box\brsp100\brdrth {\f22\fs18 >188 188..733\par +}\pard \li1260\ri1360\sl220\box\brsp100\brdrth {\f22\fs18 TMEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSAAVIKWYAERDAEIEN\par + EKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNARDSAEVVETAFCTFVLS\par + RIAGEIASILDGLPLSVQRRFPELENRHVDFLKRDIIKAMNKAAALDELIPGLLSEYIEQ\par + SG*\par +>711 711..2633\par + VNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKESAYQEGRWETLPFQRAI\par + MNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTLIWLPTDGDAENFMKTHVE\par + PTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLGGKAAKNYREKSVDVAGYDEL\par + AAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVRGTCQIERAASESPHFMRFHVAC\par + PHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEHNACVIRQQELDFTDARYICEKTGI\par + WTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFTTWVQIVKDWMKTKGDTGKRKTFVNTT\par + LGETWEAKIGERPDAEVMAERKEHYSAPVPDRVAYLTAGIDSQLDRYEMRVWGWGPGEES\par + WLIDRQIIMGRHDDEQTLLRVDEAINKTYTRRNGAEMSISRICWDTGGIDPTIVYERSKK\par + HGLFRVIPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLP\par + GAVHFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKKRRNEALDCFVYALAALRI\par + SISRWQLDLSALLASLQEEDGAATNKKTLADYARALSGEDE*\par +>74 complement(74..727)\par + LFDIFTQQPRYQFIQRGCFVHGFDDIPFQEINMSVFQFRKTPLHRQGEPVENTGNFTCDP\par + RQHESTECGFHHFSGVSGILQFLCVGLRTRKSMAFVLNSSWLEICLAGLPQFFNLPAQLF\par + VLNFSIPFGIPFYDGGRVIKHLITLATASQNGHSLFLPVLNGTDTRTENVSQLLFVDFHC\par + SFHGQKQRKETTEAKKPRFQHLSFPFFSEGILNKNIKL*\par +>313 complement(313..732) \par + PDCSIYSLSNPGISSSSAAALFMALMISRFRKSTCRFSSSGKRRCTDRGSPSRILAISPA\par + IRDSTKVQNAVSTTSAESLAFFSSCASACARVSRWRSYSIVPGWRSASLACRSSSTSRRS\par +}\pard \li1260\ri1360\sl220\box\brsp100\brdrth {\f22\fs18 FSFSISASLSAYHFMTAAES*\par +}\pard \li1260\ri1360\sl220 {\f22\fs18 \par +}\pard\plain \s8\qj\fi-1140\li1140\sb120\sa240\sl240\tx1140 \f21\fs20 Figure 10.3\tab The contents of a file containing the protein sequences of the open reading frames found by the program\par +\pard\plain \s6\fi-560\li560\sb240\sa60\sl280\tx560 \b\f20 2.6\tab Producing a file of protein sequences for segments defined from a feature table\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Translate and write protein sequences to disk".\par +2.\tab Accept "Translate selected regions".\par +3.\tab Reject "Define segments using keyboard". The alternative is to use a feature table.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Feature table file name". Type the name of the file containing the appropriate feature table in EMBL/GenBank format.\par +5.\tab Define "Operator". This defines which feature table operators should be employed when selecting the segments to translate.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Define "File name for translation"\par +\pard\plain \s4\qj\sa120\sl280 \f20 The program will now read the feature table file and translate the segments defined using the selected operator(s). The results will be stored as in figure 10.3.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 3.\tab Notes\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab To produce a listing without translation the "Translate and list" function can be used with the "Show translation" option rejected. Alternatively the function "List the sequence" can be used. +\par +2.\tab Some users may be confused by the fact that the program asks "Where to list from, and to" and also "Define segments to translate". This allows for 5' and 3' untranslated regions to be included in the listing.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +The feature table file employed by the programs is a simple text file containing the data for the current sequence. Because of the multiplicity of different sequence library formats we have not provided the facility of reading such data directly from li +braries. The feature tables for individual library entries must be extracted (see the introductory chapter) or files can be created for new sequences.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab +The current feature tables use "operators" such as "join" or "order" to specify which segments should be translated together to make a complete protein sequence. The program allows users to select which ones to employ, the default being "Use all operato +rs".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab The program contains a function "Set genetic code" which allows users to choose from a menu of codes or to define their own by specifying amino acid and codon pairs. This sets the code for all functions. +\par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 11. Statistical and Structural Analysis of Protein Sequences\par +\pard\plain \s3\sb200\sa120\sl360 \b\f20\fs32 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +2.\tab Methods\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.1\tab Plotting hydrophobicity\par +2.2 \tab Plotting charge\par +2.3\tab Plotting hydrophobic moment and hydrophobicity\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700\tx1980 2.4\tab Drawing helical wheels\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.5\tab Producing a Robson secondary structure prediction\par +2.6\tab Calculating the amino acid composition and molecular weight\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Notes\par +4.\tab References\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 In this chapter we describe the use of routines for plotting hydrophobicity, charge and hydrophobic moments, drawing helix wheels and predicting second +ary structure. Use of all these routines is very straightforward and they are contained in the program PIP.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 2.\tab Methods\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.1\tab Plotting hydrophobicity\par +\pard\plain \s4\qj\sa120\sl280 \f20 This method uses the values of Kyte and Doolittle (1)\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Plot hydrophobicity".\par +2.\tab Define "Window length".\par +3.\tab Define "Plot interval".\par +\pard\plain \s4\qj\sa120\sl280 \f20 The plot will appear as in figure 11.1.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.2\tab Plotting charge\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Plot charge".\par +2.\tab Define "Window length".\par +3. \tab Define "Plot interval".\par +\pard\plain \s4\qj\sa120\sl280 \f20 The plot will appear and will be similar to that shown in figure 11.1.\par +\pard\plain \sl220\keepn \f4\fs16 {{\pict\macpict\picw448\pich81 +0396ffffffff005001bf1101a0008201000affffffff005001bf0900000000000000003100000000004f01be9800240000000000350120000000000035011f00000000004f01be000102dd0006007fdfff00fc060040df000004060040df000004060040df000004060040df000004060078df000004060040df0000040600 +40df000004060040df000004060040df00000407017840e0000004070140b0e000000407014108e000000407014104e000000407014204e00000040b017a02fc000020e60000040c014202fd00010250e60000040f014402fd00010590e900031000000418014401fd00010490f800010380f8000020fe0003700000041c01 +4801fd00010808f800010480fd000010fd000060fe0003880000041d017801fd00010808f800010440fe00010428fd000090fe00038804000424074801000002000804fe00010110fd00010440fe00010a28fe000801108004010806000424075000800005001004fe000101a8fd00010840fe000d09440000020111800b01 +04090004240c500080000480100200800002a4fd00010840fe000d10c40020030a0a4009010709002425236000800004e0100201400002440020000210400004401002005004960c400882010881e42523780080000810100202300002430050000d1020001ba0200100900490004810820090412425234000800008102001 +02080002008088001120200020104000811004600037f0c20090222406007fdfff00fc2523400040002008e00104032c040022020020c0180080038000420808000020000c00600a14241440004000200500010400c204001201002080080080fe0002420410fd000404004004142113780030004005000084000104001401 +0040000401fd0002220410fb00024004141f13400008004005000098000088000c010040000401fd0002240410f900000c1f134000080040020000e00000900000010040000407fd00021c0220f90000041c05400009008002fc0008600000010e80000208fc000103e0f900000416044000068080fb000040fe0004918000 +0210f2000004110378000441f6000490800003f0f20000040d0340000022f60000a0ee0000040d0340000022f6000060ee000004090340000014e2000004090340000008e2000004060078df000004060040df000004060040df000004060040df000004060040df000004060078df000004060040df000004060040df0000 +04060040df000004060040df00000406007fdfff00fc02dd00a00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 11.1\tab A hydrophobicity plot using the values of Kyte and Doolittle.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.3\tab Plotting hydrophobic moment and hydrophobicity\par +\pard\plain \s4\qj\sa120\sl280 \f20 This method plots the hydrophobic moment and the hydrophobicity as defined by Eisenberg {\i et al} (2).\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Plot hydrophobic moment".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Define "Angle". This is the angle between the residues when the helix is viewed end on. The default value of 100 degrees is that found in alpha helices.\par +3.\tab Define "Window length". The default of 18, if used in conjunction with the default "Angle", is equivalent to 5 turns of the helix.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Plot interval".\par +\pard\plain \s4\qj\sa120\sl280\tx560 \f20 +The plot will appear as in figure 11.2. with the hydrophobicity shown above the hydrophobic moment. The scale for the hydrophobicity runs from -1.0 to 1.5 and for the hydrophobic moment from 0.0 to 1.5. The program plots the mean values for each window pos +ition with the value at position x representing the segment from x-window length+1 to x.\par +\pard\plain \sb200\sl220\keepn \f4\fs16 {{\pict\macpict\picw447\pich160 +0659ffffffff009f01be1101a0008201000affffffff009f01be0900000000000000003100000000009e01bd9800240000000000670120000000000067011f00000000009e01bd000102dd0006007fdfff00fc060040df000004060070df000004060040df000004060070df000004060040df000004060070df0000040600 +40df000004060070df000004060040df00000406007edf000004060040df000004060070df000004060040df000004060070df000004060040df000004060078df000004060074df0000040a0072fc000008e50000040a0061fc000038e50000040e007ffc000044f2000020f500000413016080fd000084f2000050f90000 +08fe00000414017080fe00010104f2000048f9000016fe00000419016040fe00010202f2000088fe000001fe000502110008000419017030fe00010401f2000084fe000902818000052088140004200c40080001000401e00800000380f900012102fe00090242600004e096120004240c70080002800800101400000c40fd +000008fe000e320380000004741000040061210004240c40080004800800082200003040fd000034fe000e4a004000001c0c0c00080001210004250c70040004500800042200004020fe00131c440000e04c0020000020000b0e080000c08004250c400400086f10000241f8038010fe001323820001104000100000400000 +91100000808004241e7e02000800f00001800404001100001c4002000e0880000c400080000060e0fd0000041f0340020008fd0012800208000ef000244002001004800003b00080f90000041e0370010010fc000b023800000fc0228001001003fe0002080080f90000041c0340010010fc000101c0fe00053042800100a0 +fd00010801f8000004170370008020f70005084280008160fd00010401f8000004160340008040f700040481000041fc000107c6f8000004150370004440f700040301000021fb000028f8000004110340004a80f300001afb000010f80000041002700031f2000006fb000010f8000004060040df00000406007edf000004 +060040df000004060070df000004060040df000004060070df000004060040df000004060070df000004060040df000004060070df000004060040df00000406007fdfff00fc060040df000004060040df000004060070df000004060040df000004060040df000004060040df000004060070df000004060040df00000406 +0040df000004060070df000004060040df000004060040df000004060070df000004060040df000004060040df000004060040df00000406007edf000004060040df000004060040df000004060070df000004060040df000004060040df000004060070df000004060040df000004060040df000004060040df0000040600 +70df0000040a0040f6000010eb0000040a0040f600002ceb0000040a0070f6000024eb0000040a0040f6000042eb0000040c0040f80002800082eb00000411007efe000040fd000303400082eb000004110040fe0000a0fd000304400101eb000004130040fe0000a0fd0005082002010001ed0000041e044000000110fd00 +070820040100030010f9000040fe000103e0fd0000042204700000011cfd000730100800c0048128fd000480000004c0fe00010410fd00000423044000000204fd000720081000200482c4fe00050380000006a0fe00012808fd00000424044000000402fe0008204008100020088404fe0005024000000920fe00015808fd +00000424047000001c02fe0008504004100020084404fe0005044000004920fe00018004fd00000425104000002002700000484004200010084802fe000f84400000a820040001000400e00000042523400000200190000048800420001010480200000144420000b0100a018a000403100080042523700001200109000084 +80044000087030020000024826000110105206740002241003000425234001c2c0000a8008848002800008801002000804482508071010b208000003d812040004251b40022440000a601703000280000500000104340428291408000d0108fe0004080d080004241b60041800000411a00200030000070000012a44043019 +240800030108fd0003088800041e077f88100000040a60f9000701d982880000c408fe000090fc00025000041b014050fd00000cf7000601037800008210fe000070fc00025000040e014030ed000103f0f8000220000406007fdfff00fc02dd00a00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 11.2\tab A hydrophobic moment (below) and hydrophobicity plot. The hydrophobicity plot displays the mean va +lues on a scale of -1.5 to 1.0 and the hydrophobic moment on a scale of 0.0 to 1.5.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.4\tab Drawing helical wheels\par +\pard\plain \s4\qj\sa120\sl280 \f20 This method draws helical wheels for any segment of the sequence (3). In addition it displays the hydrophobic moment for the segment (2).\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Draw helix wheel".\par +2.\tab Define "Angle". The default angle of 100 degrees is that found in alpha helices.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Define "Window length". The default of 18, if used in conjunction with the default "Angle", is equivalent to 5 turns of the helix.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Step +". To produce a display for a sequence position N bases from the current one type N, and the display will appear in place of the previous one. The default value of N is 1, so by repeatedly hitting carriage return the user can step, residue by residue, thro +ugh the sequence.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The display for the current position in the sequence will appear as in figure 11.3. and the bell will ring. The program now allows the user to "step through the sequence displaying the helix wheel for each position. +\par +\pard\plain \li900\ri960\sb500\sl220\keepn\box\brsp120\brdrth \f4\fs16 {{\pict\macpict\picw355\pich329 +0c64ffffffff014801621101a00082a0008c01000affffffff0148016209000000000000000031010f01050121011338a10096000c010000000200000000000000a1009a0008fffd00000004000001000a01100106011e01112c000c00150948656c76657469636103001504010d000c2e00040000010028011a01070144a0 +0097a0008da0008c01000affffffff0148016231012600ba013800c838a10096000c010000000200000000000000a1009a0008fffc00000004000001000a012700bb013500c628013100bc014ca00097a0008da0008c01000affffffff0148016231011d0087012e009538a10096000c010000000200000000000000a1009a +0008fffc00000004000001000a011d0088012b009328012700890146a00097a0008da0008c01000affffffff014801623100df004600f1005438a10096000c010000000200000000000000a1009a0008fffd00000004000001000a00e0004700ee00532800ea00480156a00097a0008da0008c01000affffffff0148016231 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+3138a10096000c010000000200000000000000a1009a0008fffd00000005000001000a00f40124010201302b9ac00153a00097a0008d01000affffffff0148016207000000002200bc01210000a000a0a100a4000209fd01000a0000000000000000070001000109ffffffffffffffff22005900bf62632300002100fc009f +23000023cc8723000021006d00fe2300002100ee00fe2300002100d8006b23000023338723000021009f0120230000239f6323000023a29c23000021005f00e2230000233278230000a000a301000affffffff0148016222005900bf62632100fc009f23cc8721006d00fe2100ee00fe2100d8006b23338721009f0120239f +6323a29c21005f00e2233278a000a1a10096000c030000000200000000000000a1009a0008fffc00000003000001000a002000f9003101020d000e28002c00fa012ba00097a10096000c030000000200000000000000a1009a0008fffc00000003000001000a002100820032008b28002d0083012ba00097a10096000c0300 +00000200000000000000a1009a0008fffc00000003000001000a0096015800a701612bd675012ba00097a10096000c030000000200000000000000a1009a0008fffc00000003000001000a00b7015700c801602800c30158012ba00097a10096000c030000000200000000000000a1009a0008fffc0000ffff000001000a00 +4401250055012f280050012a012da00097a10096000c030000000200000000000000a1009a0008fffc0000ffff000001000a001900b7002a00c128002500bc012da00097a10096000c030000000200000000000000a1009a0008fffc0000ffff000001000a011d0107012e0111280129010c012da00097a10096000c030000 +000200000000000000a1009a0008fffc0000ffff000001000a013600b6014700c028014200bc012ea00097a10096000c030000000200000000000000a1009a0008fffc0000ffff000001000a012a007c013b00862801360082012ea00097a10096000c030000000200000000000000a1009a0008fffc0000fffe000001000a +00e4003100f5003b2800f00037012ea00097a10096000c030000000200000000000000a1009a0008fffc0000ffff000001000a0092002400a3002e28009e002a012ea00097a10096000c030000000200000000000000a1009a0008fffc0000ffff000001000a005a003e006b00472800660043012ea00097a00083ff}} +\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa240\sl240\tx1140 \f21\fs20 Figure 11.3\tab A typica +l helix wheel display using a window of only 13 residues. The display includes a schematic of the helix showing the links between residues, with each vertex numbered according to position; the residue type at each vertex; a symbol denoting a classification + as hydrophobic (.), positively charged (+), negatively charged (-), or otherwise (). The residue number of the first sequence element in the current window is displayed at the top left corner along with the sequence. Below this is the total hydrophobicity + and hydrophobic moment according to Eisenberg {\i et al }(2).\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.5\tab Producing a Robson secondary structure prediction\par +\pard\plain \s4\qj\sa120\sl280 \f20 This method uses the method of Garnier {\i et al} (4) to predict the positions of alpha helices, beta sheets, turns and random coil. The results can be either plotted or listed.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Robson secondary structure prediction".\par +\pard \s7\qj\fi-560\li560\ri-100\sa120\sl280\tx560 \page 2.\tab Accept "Plot results". The alternative produces a listing like that shown in figure 11.4.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The plot will appear as in figure 11.5. and the program also prints a count of the number of positions at which each of the 4 structure types is the highest scoring.\par +\pard\plain \li1500\ri1460\sb200\sl220\box\brsp100\brdrth \tqr\tx3220\tqr\tx4700\tqr\tx6140\tqr\tx7420 \f4\fs16 350 P\tab 274\tab -178\tab -84\tab -77\par +\pard \li1500\ri1460\sl220\box\brsp100\brdrth \tqr\tx3220\tqr\tx4700\tqr\tx6140\tqr\tx7420 351 L\tab 16\tab -192\tab -21\tab -38\par +352 K\tab 371\tab -223\tab -75\tab -68\par +353 L\tab 365\tab -152\tab -101\tab -65\par +354 S\tab 331\tab -82\tab -84\tab -63\par +355 K\tab 311\tab -43\tab -110\tab -88\par +356 A\tab 280\tab -23\tab -110\tab -80\par +357 V\tab 234\tab -12\tab -135\tab -75\par +358 H\tab 177\tab -10\tab -143\tab -92\par +359 K\tab 153\tab 2\tab -180\tab -138\par +360 A\tab 158\tab 52\tab -175\tab -130\par +361 V\tab 144\tab 78\tab -187\tab -115\par +362 L\tab 132\tab 58\tab -186\tab -80\par +363 T\tab 124\tab 63\tab -142\tab -78\par +364 I\tab 144\tab 32\tab -111\tab -43\par +365 D\tab 120\tab -49\tab -29\tab 5\par +366 E\tab 103\tab -80\tab 13\tab 43\par +367 K\tab 111\tab -113\tab 23\tab 42\par +368 G\tab 132\tab -127\tab -13\tab 64\par +369 T\tab 172\tab -132\tab -42\tab 52\par +\pard \li1500\ri1460\sl220\keepn\box\brsp100\brdrth \tqr\tx3220\tqr\tx4700\tqr\tx6140\tqr\tx7420 370 E\tab 216\tab -170\tab -122\tab -4{\b \par +}\pard\plain \s8\qj\fi-1140\li1140\sb120\sa200\sl240\tx1140 \f21\fs20 Figure 11.4\tab A listing of the Robson secondary structure prediction. It includes the sequence position, the residue type and the values for the four structure classes.\par +\pard\plain \sb200\sl220\keepn \f4\fs16 {{\pict\macpict\picw446\pich256 +0d0fffffffff00ff01bd1101a0008201000affffffff00ff01bd090000000000000000310000000000fe01bc9800240000000000a601200000000000a6011f0000000000fe01bc000102dd0006007fdfff00fc060040df000004060040df000004060040df000004060041df00000407014280e0000004060042df0000040b +0042fd00010140e500000410014380fe000101a0fd0000c0ea000004110640000008800120fe000101a0ea000004160c40000019900124000010022380fc00000cf1000004200c40000066e80216000070022240fc000012fe00014001fc000060fd00010404240e400000a68804190000908214400006fe000612200000a0 +0180fe00010190fd00010c04252340001100080409020111421820000b02800012500000a181900000040f10001006001204252340003f0008040903c20d3c0020001103c00022900000a1827900038e080800380a90120425234061410004340104a205200010021084200022910000a272279ea495300800440ba8218425 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+40fe000cf000000e1001f800000b980c04060040df000004060040df000004060040df000004060040df000004060040df0000042406407000eee000e0fe0011032380000001c00038800001e10100000208fd000304201204060040df000004060040df000004060040df000004060040df000004060040df000004060040 +df0000042202439f80fe000018fd00111e200010061f0260000c000e1e000001f7fefc00010184060040df000004060040df000004060040df000004060040df000004060040df000004252340005f0007fc01ffff0ffc001fffe3f801fd81bff3fe3801fffff800000ffffff8cfe004060040df000004060040df00000406 +0040df000004060040df000004060040df000004060040df00000406007fdfff00fc02dd00a00083ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 11.5\tab A secondary structure plot using the method of Robson. The likelihood that each 17 residue segment of the sequence forms one of the four structure classes\: + helix (H), extended (E) normally termed sheet, turn (T) and coil (C) are each plotted out across the screen in four strips. Below this +is a "decision" strip (D) in which a single dot is poltted for the higest scoring structure class at each point. Here we see a sequence that is predicted to be predominantly helical.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.6\tab Calculating the composition and molecular weight of a sequence.\par +\pard\plain \s4\qj\sa120\sl280 \f20 +Select "Count amino acid composition". The composition and molecular weight are displayed as in figure 11.6.. Each column contains the one letter code for the amino acid, the number of occurrences of that amino acid in the sequence, and the number expresse +d as a percentage, and its molecular weight.\par +\pard\plain \li220\ri280\sb200\sl220\box\brsp100\brdrth \f4\fs16 Sequence composition\par +\pard \li220\ri280\sl220\box\brsp100\brdrth A C S T P A G N D E Q B Z H\par +N 0. 14. 19. 12. 30. 26. 3. 10. 11. 4. 0. 0. 0.\par +% 0.0 5.3 7.3 4.6 11.5 9.9 1.1 3.8 4.2 1.5 0.0 0.0 0.0\par +W 0. 1219. 1921. 1165. 2132. 1483. 342. 1151. 1420. 513. 0. 0. 0.\par +\par +A R K M I L V F Y W - X ? \par +N 7. 7. 10. 15. 39. 23. 13. 11. 8. 0. 0. 0. 0.\par +% 2.7 2.7 3.8 5.7 14.9 8.8 5.0 4.2 3.1 0.0 0.0 0.0 0.0\par +W 1093. 897. 1312. 1697. 4413. 2280. 1913. 1795. 1490. 0. 0. 0. 0.\par +\pard \li220\ri280\sl220\keepn\box\brsp100\brdrth Total molecular weight= 28256.254\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 11.6\tab A typical molecular weight and composition display. It includes the residue type, their number, their percentage and their contribution to the molecular weight.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 3.\tab Notes\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab The methods described in the chapters on motif and pattern searching can also be used to search for specifi +c structures. For example a sequence can be searched for all the structures contained in the PROSITE motif library.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab It is often convenient to produce displays in which several of the plots described above appear together on the screen.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 4.\tab References\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Kyte, J. and Doolittle, R.F. 1982. A simple method for displaying the hydropathic character of a protein. {\i J.Mol. Biol}. {\b 157}\:105-132. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Eisenberg, D., Schwarz, E., Komaromy, M., and Wall, R. 1984. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. {\i J. Mol. Biol.} {\b 179}\:125-142.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Schiffer,M and Edmundson,A.B. 1967 Use of helical wheels to represent the structures of proteins and to identify the segments with helical potential. {\i Biophys. J}. {\b 7}, 121-135.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Garnier, J., Osguthorpe, D.J., and Robson, B. 1978. Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins. {\i J. Mol. Biol}. {\b 120}\: +97-120.\par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 12. Searching for Motifs in Protein Sequences\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +2.\tab Methods\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.1\tab Searching for exact matches.\par +2.2\tab Searching for percentage matches to consensus sequences\par +2.3\tab Searching for consensus sequences using a score matrix\par +2.4\tab Using weight matrices for searching protein sequences\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Notes\par +4.\tab References\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The program PIP contains several ways of defining and searching for motifs (1,2). We describe searches for exact matches and percentage matches, the use of score matrices and the creation and use of weight matrices. All of the searches produce +both listed and graphical output.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 2.\tab Methods\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.1\tab Searching for exact matches.\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The routine for finding and displaying the positions of exact matches to sequences can display its results in various forms. It is equivalent to the restriction enzyme search routine in the nucleotide analysis programs. The sequences to be searched for ca +n be typed on the keyboard or read from files. The format of these files is given in the notes. Here we give only a single example of the use of the routine which shows how to produce a plot of the positions of all amino acid types in a sequence.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Search".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab +Select "Input source" as "All acids file". A number of standard files are available and users may also have their own. The one selected simply contains the one letter codes for all the standard amino acids.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Accept "Search for all names". The alternative allows users to select a subset of the entries in the file by name.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Select "Order results name by name".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Reject "List matches". If results are listed the output gives the name and position of each match and also the separations between matches.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The results will then appear in the form shown in figure 12.1. \par +\pard\plain \li80\ri80\sl220\keepn\box\brsp40\brdrth \f4\fs16 {{\pict\macpict\picw441\pich182 +14a4ffffffff00b501b81101a0008201000affffffff00b501b8090000000000000000310000000000b201b798002a000000000083014400000000008301440000000000b201b7000102d70020f90002020080fd000a8000008000010204200201fb000620000200401004fe0020f90002020080fd000a8000008000010204 +200201fb000620000200401004fe00220050fa0002020080fd000a8000008000010204200201fb000620000200401004fe00220050fa0002020080fd000a8000008000010204200201fb000620000200401004fe00220070fa0002020080fd000a8000008000010204200201fb000620000200401004fe00220020fa000202 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Figure 12.1\tab Typical graphical output from "Search for exact matches" in which the position of each matching string (here individual amino acid types) is marked.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.2\tab Searching for percentage matches to sequences\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Find percentage matches".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Accept "Type in strings". The alternative allows the string to be extracted from a named file.\par +3.\tab Reject "Keep picture". This will cause the graphics window to be cleared. The alternative leaves it unchanged.\par +4.\tab Define "String". Type in the search string. When the program cycles round to this point again the previous string will be offered as a default.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Define "Percent match". The search is performed, the results are presented graphically, the number of matches displayed, and the scores and positions of the top 10 matches displayed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Define the number of matches to "Display". For the number of matches chose +n the program will display the search string and matching sequence written one above the other with matching characters indicated by asterisk symbols. The program now cycles round to step 3.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.3\tab Searching for sequences using a score matrix\par +\pard\plain \s4\qj\sa120\sl280 \f20 +A score matrix gives a score for the alignment of each possible pair of sequence symbols. This method is more sensitive than the simple percentage match search. The default matrix MDM78 used by this program is shown in figure 12.2.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Find matches using a score matrix".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Accept "Type in strings". The alternative allows the string to be extracted from a named file.\par +3.\tab Reject "Keep picture". This will cause the graphics window to be cleared. The alternative leaves it unchanged.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab +Define "String". Type in the search string. When the program cycles round to this point again the previous string will be offered as a default. The program displays the minimum and maximum possible scores for the string.\par +5.\tab Define "Score". The search is performed, the results are presented graphically, the number of matches displayed, and the scores and positions of the top 10 matches displayed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab +Define the number of matches to "Display". For the number of matches chosen the program will display the search string and matching sequence written one above the other with matching characters indicated by asterisk symbols. The program now cycles round + to step 3. An example run is shown in figure 12.3.\par +\pard\plain \li220\ri280\sb200\sl220\box\brsp100\brdrth \f4\fs16 C S T P A G N D E Q B Z H R K M I L V F Y W - X ? \par +\pard \li220\ri280\sl220\box\brsp100\brdrth C 22 10 8 7 8 7 6 5 5 5 5 5 7 6 5 5 8 4 8 6 10 2 10 10 10 10\par +S 10 12 11 11 11 11 11 10 10 9 10 10 9 10 10 8 9 7 9 7 7 8 10 10 10 10\par +T 8 11 13 10 11 10 10 10 10 9 10 10 9 9 10 9 10 8 10 7 7 5 10 10 10 10\par +P 7 11 10 16 11 9 9 9 9 10 9 10 10 10 9 8 8 7 9 5 5 4 10 10 10 10\par +A 8 11 11 11 12 11 10 10 10 10 10 10 9 8 9 9 9 8 10 6 7 4 10 10 10 10\par +G 7 11 10 9 11 15 10 11 10 9 10 10 8 7 8 7 7 6 9 5 5 3 10 10 10 10\par +N 6 11 10 9 10 10 12 12 11 11 12 11 12 10 11 8 8 7 8 6 8 6 10 10 10 10\par +D 5 10 10 9 10 11 12 14 13 12 13 12 11 9 10 7 8 6 8 4 6 3 10 10 10 10\par +E 5 10 10 9 10 10 11 13 14 12 12 13 11 9 10 8 8 7 8 5 6 3 10 10 10 10\par +Q 5 9 9 10 10 9 11 12 12 14 11 13 13 11 11 9 8 8 8 5 6 5 10 10 10 10\par +B 5 10 10 9 10 10 12 13 12 11 13 11 11 10 10 8 8 6 8 5 7 4 10 10 10 10\par +Z 5 10 10 10 10 10 11 12 13 13 11 14 12 10 10 8 8 8 8 5 6 4 10 10 10 10\par +H 7 9 9 10 9 8 12 11 11 13 11 12 16 12 10 8 8 8 8 8 10 7 10 10 10 10\par +R 6 10 9 10 8 7 10 9 9 11 10 10 12 16 13 10 8 7 8 6 6 12 10 10 10 10\par +K 5 10 10 9 9 8 11 10 10 11 10 10 10 13 15 10 8 7 8 5 6 7 10 10 10 10\par +M 5 8 9 8 9 7 8 7 8 9 8 8 8 10 10 16 12 14 12 10 8 6 10 10 10 10\par +I 8 9 10 8 9 7 8 8 8 8 8 8 8 8 8 12 15 12 14 11 9 5 10 10 10 10\par +L 4 7 8 7 8 6 7 6 7 8 6 8 8 7 7 14 12 16 12 12 9 8 10 10 10 10\par +V 8 9 10 9 10 9 8 8 8 8 8 8 8 8 8 12 14 12 14 9 8 4 10 10 10 10\par +F 6 7 7 5 6 5 6 4 5 5 5 5 8 6 5 10 11 12 9 19 17 10 10 10 10 10\par +Y 10 7 7 5 7 5 8 6 6 6 7 6 10 6 6 8 9 9 8 17 20 10 10 10 10 10\par +W 2 8 5 4 4 3 6 3 3 5 4 4 7 12 7 6 5 8 4 10 10 27 10 10 10 10\par +- 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10\par +X 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10\par +? 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10\par +\pard \li220\ri280\sl220\keepn\box\brsp100\brdrth 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa300\sl240\tx1140 \f21\fs20 Figure 12.2\tab The amino acid score matrix MDM78.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.4\tab Using weight matrices for searching protein sequences\par +\pard\plain \s4\qj\sa120\sl280 \f20 +A weight matrix is the most sensitive way of defining a motif. It is a table of values that gives scores for each amino acid type in each position along a motif. For a motif of length 8 amino acids the weight matrix would be a table 8 positions long and, a +llowing for 26 amino acid symbols, 26 deep. The simplest way of choosing the values for the table is to take an alignment of all known +examples of the motif and to count the frequency of occurrence of each amino acid type at each position. These frequencies can be used as the table of weights. When the table is used to search a new sequence the program calculates a score for each position + along the sequence by adding or multiplying (see notes) the relevant values in the table. All positions that exceed some cutoff score are reported as matching the original set of motifs.\par +\pard \s4\qj\sa120\sl280 How can we select a suitable cutoff score? The simplest way is to ap +ply the weight matrix to all the known occurrences of the motif - i.e. the set of sequence segments used to create the table - and to see what scores they achieve. The cutoff can be selected accordingly. For convenience the weight matrix is stored as a fil +e along with its cutoff score, a title that is displayed when the file is read, and a few other values need by the program. A routine for creating weight matrix files from sets of aligned sequences is included in the program. When a search using the weight + matrix is performed the program will either list the matching sequence segments or plot their positions as for the other motif search methods.\par +\pard\plain \li2000\ri2260\sb200\sl220\box\brsp100\brdrth \f4\fs16 Find matches using a score matrix\par +\pard \li2000\ri2260\sl220\box\brsp100\brdrth ? Keep picture (y/n) (y) =\par + ? String=ALPHA\par +Minimum score= 23 Maximum score= 72\par +? Score (23-72) (72) =60\par +\par +For score 60 the number of matches= 5\par +Scores 62 62 62 61 61\par +Positions 120 217 420 54 326\par +? Display (0-5) (0) =\par +\par + 120\par + PLDHD\par + * *\par + ALPHA\par + 1\par +\par + 217\par + ALANT\par + **\par + ALPHA\par + 1\par +\par + 420\par + QLDHG\par + * *\par + ALPHA\par + 1\par +\par + 54\par + SLPGN\par + **\par + ALPHA\par + 1\par +\par + 326\par + ALPII\par + ***\par + ALPHA\par + 1\par +? Keep picture (y/n) (y) =\par + Default String=ALPHA\par +\pard \li2000\ri2260\sl220\keepn\box\brsp100\brdrth ? String=!\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa420\sl240\tx1140 \f21\fs20 Figure 12.3\tab An example of the listed output from "Search using a score matrix".\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.4.1\tab Creating a weight matrix file from a set of aligned sequences\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Motif search using weight matrix".\par +2.\tab Select "Make weight matrix".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +Define "Name of aligned sequences file". We assume the file of aligned sequences has already been created (see note 5). The program reads and displays the contents of the file numbering each sequence as it goes. Then it displays the length of the longes +t sequence.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Accept "Sum logs of weights". The alternative is to sum the weights when calculating scores (see note 6). \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Accept "Use all motif positions". The alternative allows the user to define a "mask" which i +dentifies positions within the motif that should be ignored when the matrix is created (see note 7). The program now calculates the weights and applies them in turn to each of the sequences in the file. The number and score for each sequence is displayed, +followed by the top, bottom and mean scores and the standard deviation. In addition the mean plus and minus 3 standard deviations is displayed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Define "Cutoff score". The default is the mean minus 3 standard deviations, but users may, for example, decide to use the lowest score obtained by the sequences in the file.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Define "Top score for scaling plots". This parameter is used by the graphics output routine when scaling the plots. Its value will influence the height of lines plotted to represent matches.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 8.\tab +Define "Position to identify". When a search is performed it is not always appropriate to report the position of a match relative to the leftmost amino acid in the motif. For example when performing a helix-turn-helix motif search we may want to know + the position of the well conserved glycine rather than the position of the first amino acid in the matrix. The "Position to identify" allows the user to define which amino acid is marked. The amino acids in the table are number 1,2,3 and so on.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 10.\tab Define a "Title". This is a title that will be displayed when the matrix file is read prior to performing a search. It is limited to 60 characters.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 11.\tab Define "Name for new weight matrix file". Give a name for the weight matrix file.\par +\pard\plain \s4\qj\sa120\sl280 \f20 See the example run in figure 12.4.\par +\pard\plain \li1240\ri1180\sb300\sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth \f4\fs16 Motif search using weight matrix\par +\pard \li1240\ri1180\sl220\brdrl\brsp100\brdrth \brdrr\brsp100\brdrth Select operation\par +X 1 Use weight matrix\par + 2 Make weight matrix\par + 3 Rescale weight matrix\par +? Selection (1-3) (1) =2\par +? Name of aligned sequences file=atpbinding.seq\par + 1 GETLGIVGESGSG\par + 2 GESLGVVGESGGGKSTFAR OppF\par + 3 GDVISIDGSSGSGKSTFLR HisP\par + 4 GEFVVFVGPSGGGKSTLLR MalK E. coli\par + 5 NQVTAFIGPSGGGKSTLLR PstB\par + 6 GRVMALVGENGAGKSTMMK RbsA(N)\par + 7 GEVIGIVGRSGSGKSTLTK HlyB\par + 8 GECFGLLGPNGAGKSTITR NodI R. leguminosarum\par + 9 GEMAFLTGHSGAGKSTLLK FtsE E. coli\par + 10 GQRELIIGDRQTGKTALAI ATPase\par + 11 GGKVGLFGGAGVGKTVNMM ATPase\par + 12 GRIVEIYGPESSGKTTLTL RecA\par + 13 RSNLLVLAGAGSGKTRVLV UvrD\par + 14 GGKIGLFGGAGVGKTVGIM ATPase Bovine\par + 15 SKIIFVVGGPGSGKGTQCE Adenylate Kinase Rabbit\par + 16 NQSILITGESGAGKTVNTK Myosin Rabbit\par + 17 HVNVGTIGHVDHGKTTLTA EF-Tu E. coli\par + 18 YRNIGISAHIDAGKTTERI EF-G E. coli\par + 19 EYKLVVVGARGVGKSALTI v-ras (HARVEY)\par + 20 EYKLVVVGASGVGKSALTI v-ras (KIRSTEN)\par + 21 EYKLVVVGAVGVGKSALTI pEJ BLADDER CARCINOMA TRANSFORMING\par + 22 EYKLVVVGAGGVGKSALTI pEJ BLADDER CARCINOMA CELLULAR\par +Length of motif 19\par +? Sum logs of weights (y/n) (y) =\par + ? Use all motif positions (y/n) (y) =\par +Applying weights to input sequences\par + 1 -36.651 GETLGIVGESGSGKSQSLR\par + 2 -35.780 GESLGVVGESGGGKSTFAR\par + 3 -38.180 GDVISIDGSSGSGKSTFLR\par + 4 -35.403 GEFVVFVGPSGGGKSTLLR\par + 5 -39.039 NQVTAFIGPSGGGKSTLLR\par + 6 -40.653 GRVMALVGENGAGKSTMMK\par + 7 -34.017 GEVIGIVGRSGSGKSTLTK\par + 8 -37.454 GECFGLLGPNGAGKSTITR\par + 9 -36.474 GEMAFLTGHSGAGKSTLLK\par + 10 -43.431 GQRELIIGDRQTGKTALAI\par + 11 -40.210 GGKVGLFGGAGVGKTVNMM\par + 12 -40.720 GRIVEIYGPESSGKTTLTL\par + 13 -45.143 RSNLLVLAGAGSGKTRVLV\par + 14 -40.684 GGKIGLFGGAGVGKTVGIM\par + 15 -45.197 SKIIFVVGGPGSGKGTQCE\par + 16 -39.098 NQSILITGESGAGKTVNTK\par + 17 -43.832 HVNVGTIGHVDHGKTTLTA\par + 18 -44.817 YRNIGISAHIDAGKTTERI\par + 19 -36.305 EYKLVVVGARGVGKSALTI\par + 20 -35.101 EYKLVVVGASGVGKSALTI\par + 21 -36.305 EYKLVVVGAVGVGKSALTI\par + 22 -36.711 EYKLVVVGAGGVGKSALTI\par +Top score -34.017 Bottom score -45.197\par +Mean -39.146 Standard deviation 3.441\par +Mean minus 3.sd -49.470 Mean plus 3.sd -28.822\par +? Cutoff score (-999.00-9999.00) (-49.47) =\par +? Top score for scaling plots (-49.47-999.00) (-28.82) =\par +? Position to identify (0-19) (1) =13\par +? Title=ATP binding motif\par +\pard \li1240\ri1180\sl220\keepn\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth ? Name for new weight matrix file=atpbinding.wts\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa320\sl240\tx1140 \f21\fs20 Figure 12.4\tab An example run of the creation of a weight matrix from a set of aligned sequences.\par +\pard\plain \s9\fi-560\li860\sa60\sl280\tx1140 \b\f20 2.4.2\tab Searching using a weight matrix\par +\pard\plain \s4\qj\sa120\sl280 \f20 Once a weight matrix has been stored in a file it can be used to search any sequence. Results can be displayed graphically or the matching sequence segments can be listed out with their scores.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Motif search using weight matrix".\par +2.\tab Select "Use weight matrix".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Define "Motif weight matrix file". The name of the file containing the weight matrix. The program reads the file and displays its title.\par +4.\tab Accept "Use frequencies as weights". The alternative will use the weight matrix file as a definition of a "Membership of set" motif (see note 10).\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab +Define "Cutoff score". The default will be the value set when the weight matrix file was created. If the score is negative the program will calculate sums of logs of frequencies, otherwise it will add frequencies.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Accept "Plot results". Alternatively they will be listed.\par +The results will appear.\par +\pard\plain \s5\sa60\sl320\tx560 \b\f20\fs28 \page 3.\tab Notes\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab The files containing the definitions of peptides that can be be searched for by the exact match search routine have the following format. Each name is followed by a /, th +en each of its peptide sequences is followed by a /. The last peptide sequence for each name is followed by //. For example a file might contain the following.\par +\pard \s7\qj\li1720\sb200\sa120\sl280\tx1880 Acidic/D/E//\par +\pard \s7\qj\li1720\sa120\sl280\tx1880 Basic/R/K/H//\par +Glyco/N-S/N-T//\par +\pard \s7\qj\fi-560\li560\sb200\sa120\sl280\tx560 \tab Users could then search for these named sets of sequences. Note that the symbol - matches any amino acid.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab To search for a subset of the names in a file employed by exact match routine the user should reject "Search for all names" and the program will ask for the names wanted and extract their sequences +from the file. Alternatively, if a user was always using the same subset, then a file containing only those names could be created. This file would then be selected as "Personal file" for "Input source".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +The exact match routine also allows names and their sequences to be entered on the keyboard. This is selected as "Keyboard" for "Input source", and the program will prompt for names and their sequences. In this way the routine can be used to search for +exact matches to any short sequence. \par +4.\tab For this pr +ogram a motif is a short segment of sequence of fixed length. More complex structures termed "patterns" which we define as sets of motifs separated by varying gaps, are covered in another chapter. The current chapter should be read before the chapter on pa +tterns. \par +5.\tab The files of aligned sequences used to make weight matrices have the following format. Each sequence should be on a separate line. The sequence should start in column 2 and is terminated by a new line or a space. Anything after the space is tr +eated as a comment. The files can be created by previous searches or using an editor.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab +The frequencies in the weight matrix can be used in two ways to calculate scores for sequences. Some users prefer to add the frequencies to give a total score, and others to multiply them by summing their logs. If we regard the frequencies as probabilit +ies then multiplication seems the correct procedure. The user chooses which method will be used when the weight matrix is created, however the choice can be overridden wh +en the matrix is used. If multiplication is selected then all results will presented as sums of logs.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab +Masking the weight matrix is particularly useful in cases where a limited number of examples of a motif are available, or when the motif may have several components. In the first case the limited number of examples may make the matrix unrepresentative o +f the motif because the amino acids in the unconserved positions may bias the results of searches. We stated that a motif might have several components\: +for example it might have both structural and specificity components. We may want to separate out the two parts and again masking provides such a facility.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 8.\tab +The weight matrix handling routine contains a further option "Rescale weight matrix". If the user has edited a weight matrix to change the frequency values this provides a way of selecting a new cutoff score. It allows users to read in a set of aligned + sequences and a weight matrix and to apply the matrix to the set of sequences to see the range of scores achieved. A new weight matrix file contining the selected cutoff score is written to disk.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 9.\tab +The program contains no hardwired motifs as we expect most sites that use the programs to accumulate their own libraries of motifs and patterns, and to use the PROSITE library, both of which users can employ by simply knowing the names of the correspond +ing files.\par +10.\tab The weight matrix search can also used as a "Membership of a set" search. This means that at each position in the motif, any amino acid type tha +t is non-zero in the weight matrix is counted as a match and scores a value 1. See the chapter on searching protein sequences for patterns.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 4.\tab References\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Staden, R. 1988. Methods to define and locate patterns of motifs in sequences. {\i CABIOS} {\b 4(1)}\:53-60.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Staden, R. 1990. Searching for patterns in protein and nucleic acid sequences. (in) {\i Methods in Enzymology} R.F. Doolittle (ed.), {\b 183}\:193-211 (Academic Press, New York).\par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 13. Using Patterns to Analyse Protein Sequences\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 1.1\tab Introduction to the PROSITE motif library\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Methods\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.1\tab Creating a pattern file containing a weight matrix motif and a membership of a set motif.\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.2\tab Searching a sequence using a pattern file\par +2.3\tab Comparing a sequence against a library of patterns including PROSITE\par +2.4\tab Searching libraries for patterns\par +2.5\tab Preparing the PROSITE motif library for use by the programs\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Notes\par +4.\tab References\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 Here we describe one of the most powerful facilities provided by the program PIP\: the ability to d +efine and search sequences or libraries of sequences for complex patterns of motifs. In another chapter we give details of seaching for individual motifs but here we show how to create individual patterns and libraries of patterns and to use them to searc +h sequences. Once a pattern has been defined and stored in a file it can used to search any sequence. In addition if users want to routinely screen sequences against libraries of patterns this can be achieved by use of files of file names. For example, the + program can use the PROSITE protein motif library. The program can produce several alternative forms of output. It will display the segment of sequence matching each individual motif in the pattern, display all the sequence between and including the two o +utermost motifs, produce a description of the match in the form of a SWISSPROT feature table, or draw a simple graphical plot.\par +\pard \s4\qj\sa120\sl280 Towards the end of the chapter we describe how a related program PIPL is used to search libraries of sequences to find patterns. This program can produce alignments of sequence families.\par +\pard \s4\qj\sa120\sl280 +Patterns are defined as sets of motifs with variable spacing. Each motif in a pattern can be defined using any of several methods, and their positions relative to one other are defined in terms of minimum and maximum separations. In addition, by the use of + logical operators, each motif can be declared to be essential (the AND operator), optional (the OR operator), or forbidden (the NOT operator). The following methods (termed "classes" by the program) for defining motifs are provided\: + 1) exact match to a short sequence; 2) percentage match to a short sequence; 3) match to a short sequence using a score matrix and cutoff score; 4) match to a weight matrix; 5) direct repeat; 6) membership of a set. \par +\pard \s4\qj\sa120\sl280 +The motifs in a pattern are numbered sequentially and motif spacing is defined in the following way. When a new motif is added to a pattern the user specifies the "Reference motif" by its number and then a "Relative start position". The "Relative start pos +iti +on" is defined by taking the first base of the "Reference motif" as position 1, the next as 2, and so on. Then the user defines the allowed variation in the spacing by specifying the "Number of extra positions". Notice that the position of a motif can be d +efined relative to any other motif, and that a negative "Relative start position" declares the motif to be to the left of its "Reference motif".\par +\pard \s4\qj\sa120\sl280 The probability of finding each individual motif in the current sequence, the product of the probabilities for +all the motifs in a pattern "Probability of finding pattern", and the "Expected number of matches" is calculated and displayed by the program. In addition to the cutoffs used for the individual motifs, users can apply two pattern cutoffs\: + "Maximum pattern probability" and "Minimum pattern score".\par +\pard \s4\qj\sa120\sl280 Below we describe\: how to create a pattern; how to use a pattern file to search a sequence; how to use a "File of pattern file names" to search a sequence for a whole library of patterns; how to use a pattern file + to search a whole library of sequences; how to reformat the PROSITE motif library into a form compatible with these search programs. To describe how to create a pattern file we first show all the steps to make one containing two motifs, and then, to save +space, the parts specific to the individual motif types are sketched in the notes section.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 1.1\tab Introduction to the PROSITE motif library\par +\pard\plain \s4\qj\sa120\sl280 \f20 A library of protein motifs (in our terminology, because they include variable gaps, many would be called patterns) has + recently become available from Amos Bairoch, Departement de Biochimie Medicale, University of Geneva. Currently it contains over 500 patterns/motifs and arrives on tape or cdrom in two files\: + a .DAT file and a .DOC file. There is also a user documentation file PROSITE.USR. Here we outline the library structure and what is required to prepare the PROSITE library for use by our programs. A typical entry in the .DAT file is shown in figure 13.1. +\par +\pard \s4\qj\sa120\sl280 Each entry has an accession number (in figure 13.1 PS00197), a pattern definition (in figure 13.1 C-x(1,2)-[STA]-x(2)-C-[STA]-\{P\}-C) and a documentation file cross reference (in figure 13.1 PDOC00175). This pattern means\: + C, gap of 1 or 2, any of STA, gap of 2, C, any of STA, not P, C.\par +\pard \s4\qj\sa120\sl280 +We need to convert all of these patterns into our pattern definitions (as membership of a set, with the appropriate gap ranges) and write each into a separate pattern file with corresponding "membership of a set" weight matrices. After the conversion each +pattern file is named accession_number.pat (here PS00197.PAT). The corresponding matrix files are accession_number.wtsa, accession_number.wtsb, etc for however many are needed (here PS00197.WTSA and PS00197.WTSB)\: + two are needed because of the variable gap.\par + +n addition we can optionally split the .DAT and .DOC files into separate files, one for each entry, with names accession_number.dat and accession_number.doc. Also we create an index for the library which gives a one line description of each pattern, and en +ds with the pattern file and do +cumentation file numbers. The start of the file is shown in figure 13.2. So, refering to figure 13.2, the name of the pattern file for Glycosaminoglycan attachment site is PS00002.PAT, and for the documentation file PDOC00002.DOC\par +\pard \s4\qj\sa120\sl280 +Finally we create a file of file names for all the patterns in the library. If this file of file names is PROSITE.NAM then to use the complete PROSITE library from program PIP, users select "pattern searcher" and choose the option "use file of pattern file + names", and give the file name PROSITE.NAM. For any matches found, the accession number and pattern title will be displayed.\par +\pard\plain \li360\ri440\sl220\pagebb\box\brsp40\brdrth \f4\fs16 ID 2FE2S_FERREDOXIN; PATTERN.\par +\pard \li360\ri440\sl220\box\brsp40\brdrth AC PS00197;\par +DT APR-1990 (CREATED); APR-1990 (DATA UPDATE); APR-1990 (INFO UPDATE).\par +DE 2Fe-2S ferredoxins, iron-sulfur binding region signature.\par +PA C-x(1,2)-[STA]-x(2)-C-[STA]-\{P\}-C.\par +NR /RELEASE=14,15409;\par +NR /TOTAL=69(69); /POSITIVE=63(63); /UNKNOWN=0(0); /FALSE_POS=6(6);\par +NR /FALSE_NEG=5(5);\par +CC /TAXO-RANGE=A?EP?; /MAX-REPEAT=1;\par +CC /SITE=1,iron_sulfur; /SITE=5,iron_sulfur; /SITE=8,iron_sulfur;\par +DR P15788, FER$APHHA , T; P00250, FER$APHSA , T; P00223, FER$ARCLA , T;\par +DR P00227, FER$BRANA , T; P07838, FER$BRYMA , T; P13106, FER$BUMFI , T;\par +DR P00247, FER$CHLFR , T; P07839, FER$CHLRE , T; P00222, FER$COLES , T;\par +DO PDOC00175;\par +\pard \li360\ri440\sl220\keepn\box\brsp40\brdrth //\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 13.1\tab A typical entry from the PROSITE library\par +\pard\plain \li440\ri480\sb300\sl220\box\brsp100\brdrth \f4\fs16 IN-glycosylation site. 00001,00001\par +\pard \li440\ri480\sl220\box\brsp100\brdrth Glycosaminoglycan attachment site. 00002,00002\par +Tyrosine sulfatation site. 00003,00003\par +\pard \li440\ri480\sl220\keepn\box\brsp100\brdrth cAMP- and cGMP-dependent protein kinase phosphorylation site. 00004,00004\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa400\sl240\tx1140 \f21\fs20 Figure 13.2\tab The start of the index created by the conversion program\par +\pard\plain \s4\qj\sa120\sl280 \f20 +In order to make the PROSITE library useable by the search programs it is only necessary to run a program named SPLITP3. Two other programs, SPLITP1 and SPLITP2, only make the original files marginally easier to manage and produce an index. SPLITP1 split +s the PROSITE.DAT file to create a separate file for each entry. Each file is automatically named PSentry_number.DAT. In addition it creates an index for the library (see above).\par +\pard \s4\qj\sa120\sl280 SPLITP2 performs the same operation for the PROSITE.DOC file, except that no index is created. Files are named PSentry_number.DOC.\par +\pard \s4\qj\sa120\sl280 +SPLITP3 creates a separate pattern file and weight matrix files for each PROSITE entry from the file PROSITE.DAT. Pattern files are named PSentry_number.PAT, weight matrix files PSentry_number.WTSA, PSentry_number.WTSB, etc. The pattern title is the one li +ne description of the motif. SPLITP3 also creates a file of file names. Notice that it will ask for a path name so that the path can be included in the file of file names. This is the path to the directory in which the pattern files are stored\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 2.\tab Methods\par +\pard\plain \s6\fi-560\li560\sb240\sa60\sl280\tx560\tx920 \b\f20 2.1\tab Creating a pattern file containing a weight matrix motif and a membership of a set motif.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Pattern searcher"\par +2.\tab Select "Pattern definition mode" as "Use keyboard".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Select "Results display mode" as "Inclusive". The alternatives are listed in the introduction.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Select "Motif definition mode" as "Weight matrix"\par +5.\tab Define "Motif name". Each motif can be given an 8 character name\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Define "Weight matrix file name". Type in the name of the file containing the weight matrix. The program will display the probability of finding the motif.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 7.\tab Select "Motif definition mode" as "Membership of a set".\par +8.\tab Define "Motif name".\par +9.\tab Select "Logical operator" as "AND". The alternatives are "OR" and "NOT".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 10.\tab Select "Number of reference motif". At this stage the only choice is 1 and this is the default.\par +11.\tab Define "Relative start position". The base position relative to the "Reference motif". See the introduction.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 12.\tab Define "Number of extra positions".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 13.\tab Select input mode as "Keyboard". The alternative is an existing file in the form of a weight matrix.\par +14.\tab Define "String". Type in the sets of allowed residue types using the one letter code. See note 1\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 15.\tab Define the "Minimum matches". This is the number of positions within the motif that must match. The default is that +all positions must match but users may want to allow some flexibility by giving a lower score.\par +\tab The program now cycles round to step 7 and all subsequent passes round the loop to add further motifs to the pattern would differ only in the details for the different motif "classes".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 16.\tab Select "Pattern complete"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 17.\tab Accept "Save pattern in a file". The alternative does not save the pattern and so it can only be used once on the current sequence.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 18.\tab Define "Pattern definition file". Give a name for the new file.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 19.\tab "Define "Pattern title". All patterns can have a 60 character title that can be displayed when the pattern file is read and the sequence searched.\par +20.\tab Define "Weight matrix file name". The membership of a set motifs are stored in the form of weight matrices, and so the program needs the user to define a file name.\par +21.\tab Define "Title". Type in a title for the weight matrix like file. The title will be displayed when the file is read.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \tab The program will now display a detailed textual description of the pattern, the "Probability of finding the pattern" and the "Expected number of matches" (see figure 13.3).\par +22.\tab Define "Maximum pattern probability". Yes maximum\: any match with a greater probability of being found will be rejected. If no value is specified the search will be quicker (see notes).\par +\pard\plain \li1240\ri1360\sl220\pagebb\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth \f4\fs16 Pattern searcher\par +\pard \li1240\ri1360\sl220\brdrl\brsp100\brdrth \brdrr\brsp100\brdrth Select pattern definition mode\par +X 1 Use keyboard\par + 2 Use pattern file\par + 3 Use file of pattern file names\par +? Selection (1-3) (1) =1\par +Select results display mode\par +X 1 Motif by motif\par + 2 Inclusive\par + 3 Graphical\par + 4 SWISSPROT feature table\par +? Selection (1-4) (1) =2\par +Select motif definition mode\par +X 1 Exact match\par + 2 Percentage match\par + 3 Cut-off score and score matrix\par + 4 Cut-off score and weight matrix\par + 5 Direct repeat\par + 6 Membership of set\par + 7 Pattern complete\par +? Selection (1-7) (1) =4\par +? Motif name=atp\par +? Weight matrix file name=atpbinding.wts\par + ATP binding\par +Probability of score -47.8010 = 0.302E-04\par +Select motif definition mode\par + 1 Exact match\par + 2 Percentage match\par + 3 Cut-off score and score matrix\par +X 4 Cut-off score and weight matrix\par + 5 Direct repeat\par + 6 Membership of set\par + 7 Pattern complete\par +? Selection (1-7) (4) =6\par +? Motif name=hydro\par +Select logical operator\par +X 1 And\par + 2 Or\par + 3 Not\par +? Selection (1-3) (1) =\par +? Number of reference motif (1-1) (1) =\par +? Relative start position (-1000-1000) (20) =22\par +? Number of extra positions (0-1000) (0) =5\par +Select input mode\par +X 1 Keyboard\par + 2 File\par +? Selection (1-2) (1) =\par +Separate sets with commas\par +? String=ivl,ivl,,,rkhde\par +? Minimum matches (1.00-5.00) (3.00) =\par +Probability of score 3.000 = 0.145E-01\par +Select motif definition mode\par + 1 Exact match\par + 2 Percentage match\par + 3 Cut-off score and score matrix\par + 4 Cut-off score and weight matrix\par + 5 Direct repeat\par +X 6 Membership of set\par + 7 Pattern complete\par +? Selection (1-7) (6) =7\par +? Save pattern in a file (y/n) (y) =\par +? Pattern definition file=_paper.pat\par +? Pattern title=atpbinding plus\par +? Weight matrix file name=_hydro.wts\par +Weight matrix needs a title\par +? Title=hydrophobic and + spot\par +Pattern description\par +atpbinding plus\par +Motif 1 named atp is of class 4\par +Which is a match to a weight matrix with score -47.801\par +Motif 2 named hydro is of class 6\par +Which is membership of a set with score 3.000\par +It is anded with the previous motif.\par +Probability of finding pattern = 0.4368E-06\par +Expected number of matches = 0.1350E-02\par +? Maximum pattern probability (0.00-1.00) (1.00) =\par +? Minimum pattern score (-9999.00-9999.00) (-9999.00) =\par +{\f22\fs18 162\par +} GQRELIIGDRQTGKTALAIDAIINQR\par +Total matches found 1\par +\pard \li1240\ri1360\sl220\keepn\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth Minimum and maximum observed scores -38.35 -38.35\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa300\sl240\tx1140 \f21\fs20 Figure 13.3\tab The creation and use of a pattern containing a weight matrix motif and a membership of a set motif.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 23.\tab +Define "Minimum pattern score". A minimum pattern score only makes sense if all the motifs in the pattern are defined with compatible scoring methods. For example membership of a set motifs and weight matrices using sums of logs are incompatible. Searc +hing will now commence and any matches displayed using the chosen method. In figure 13.3 we show a typical run i +n which a pattern containing a weight matrix and a membership of a set motif is created and stored on disk. Figure 13.4 shows the contents of the pattern file. \par +\pard\plain \li2260\ri2380\sb200\sl220\box\brsp100\brdrth \f4\fs16 atpbinding plus \par +\pard \li2260\ri2380\sl220\box\brsp100\brdrth A4 atp Class \par +atpbinding.wts \par + A6 hydro Class \par + 1 Relative motif\par + 22 Relative start position\par + 5 Number of extra positions\par +\pard \li2260\ri2380\sl220\keepn\box\brsp100\brdrth _hydro.wts \par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa40\sl240\tx1140 \f21\fs20 Figure 13.4\tab The pattern file created in the worked example shown in figure 13.3.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.2\tab Searching a sequence using a pattern file\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Pattern searcher"\par +2.\tab Select "Pattern definition mode" as "Use pattern file".\par +3.\tab Select "Results display mode" as "Inclusive"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab +Define "Pattern definition file". Type the name of the file containing the pattern. The program will read the file then display its title, a detailed textual description of the pattern, the "Probability of finding the pattern", and the "Expected number +of matches".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Define "Maximum pattern probability". \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab D +efine "Minimum pattern score". Searching will now commence and any matches displayed using the chosen method. Figure 13.5 shows a typical run using a pattern file and output in the form of a SWISSPROT feature table.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 2.3\tab Comparing a sequence against a library of patterns including PROSITE\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This mode of operation allows a sequence to be searched, in turn, for any number of patterns each stored in a separate pattern file. The names of the files containing the individual patterns must be stored in a simple text +file. This file is called "a file of pattern file names" and its name is the only user input required to define the search. The file of file names could contain references to entries in the PROSITE motif library and also include the names of other patterns +.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Pattern searcher"\par +2.\tab Select "Pattern definition mode" as "Use file of pattern file names".\par +3.\tab Select "Results display mode" as "Inclusive"\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "File of pattern file names". Type the name of the file containing the list of pattern file na +mes. The program will read the file and then, in turn, all the pattern files it names. Each of these patterns will be compared against the current sequence but only those that give matches will produce any output. The pattern title and each match will be d +isplayed.\par +\pard\plain \li1240\ri1360\sb320\sl220\box\brsp40\brdrth \f4\fs16 Pattern searcher\par +\pard \li1240\ri1360\sl220\box\brsp40\brdrth Select pattern definition mode\par +X 1 Use keyboard\par + 2 Use pattern file\par + 3 Use file of pattern file names\par +? Selection (1-3) (1) =2\par +? Pattern definition file=_paper.pat\par +Select results display mode\par +X 1 Motif by motif\par + 2 Inclusive\par + 3 Graphical\par + 4 SWISSPROT feature table\par +? Selection (1-4) (1) =4\par + ATP binding sequences\par +Probability of score -47.8010 = 0.302E-04\par + hydrophobic and + spot\par +Probability of score 3.0000 = 0.145E-01\par +\par +Pattern description\par +\par + atpbinding plus\par +Motif 1 named atp is of class 4\par +Which is a match to a weight matrix with score -47.801\par +Motif 2 named hydro is of class 6\par +Which is membership of a set with score 3.000\par +It is anded with the previous motif.\par +Probability of finding pattern = 0.4368E-06\par +Expected number of matches = 0.1350E-02\par +? Maximum pattern probability (0.00-1.00) (1.00) =\par +? Minimum pattern score (-9999.00-9999.00) (-9999.00) =\par +\par +FT atp 162 187 Program\par +\par +Total matches found 1\par +\pard \li1240\ri1360\sl220\keepn\box\brsp40\brdrth Minimum and maximum observed scores -38.35 -38.35\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 13.5\tab Worked example of using a pattern file to search a sequence, and writing the results in the form of a SWISSPROT feature table.\par +\pard\plain \s6\sa60\sl280\tx560\tx860 \b\f20 \page 2.4\tab Searching libraries for patterns\par +\pard\plain \s4\qj\sa120\sl280 \f20 The program PIPL can be used to search whole sequence + libraries for patterns. Its use is similar to the pattern search routine described above, except that it does not have the facility for creating pattern files, so they must be created beforehand using PIP. In addition to its obvious application of finding + new occurrences of patterns or checking on their frequency it is a useful way of obtaining sequence alignments. It can restrict its search to a list of named entries or can search all but those on a list of entries. It can restrict its output to showing t +he highest scoring match in each sequence, but by default it will show all matches.\par +\pard \s4\qj\sa120\sl280 +Of its modes of output two require further description. The first "Padded sections" creates a new file for each match. The file will contain the sequence between and including the two outermost motifs in the pattern. It will be gapped to the furthest exten +t defined by the pattern, which means that if all the files were subsequently written one above the other all the motifs in the pattern would be exactly aligned, with the s +ections between them containing the requisite numbers of padding characters. The second such mode of output is called "Complete padded sequences". Here the user must know the maximum distance between the leftmost motif and the start of all the sequences th +at match. A trial run in which only the positions of matches are reported is usually required. The user gives this maximum distance to the program. The program then writes a new file containing the full length of all matching sequences, again maximally gap +ped (including their left ends) so that they would all align if written above one another. For both of these modes of output the files created are named "entryname" where "entryname" is the name given to the sequence in the sequence library. These modes ar +e best used with the option "Report all matches" rejected, so that only the best match for each sequence is reported. The sequences can be lined up using the sequence assembly program SAP.\par +\pard \s4\qj\sa120\sl280 The searches, which have recently been recoded, are very rapid. For + example a search of the current SWISSPROT library for a pattern defining the globin family as 6 weight matrices with widely varying gaps, finds only globins and takes less than 4 minutes using a single processor on an Alliant FX2800. This time includes re +ading in the whole library as stored in EMBL CDROM format.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select PIPL.\par +2.\tab Define "Name for results file."\par +3.\tab Select a library.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Select "Search whole library". The alternatives are "Search only a list of entries" and "Search all but a list of entries" +. The files containing the list of entries should contain one entry name per line, left justified.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab Select "Results display mode" as "Inclusive". The alternatives include "Motif by motif", "Scores only", "Complete padded sequences" and "Padded sections".\par +6.\tab Accept "Report all matches". The alternative only shows the best match for each sequence.\par +7.\tab Define "Pattern definition file". The name of the file containing the pattern created using PIP. \par +\tab The program displays a textual description of the pattern and the expected number of matches per 1000 residues assuming an average amino acid composition.\par +8.\tab Define "Maximum pattern probability". The program will run much more quickly if none is given.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 9.\tab Define "Minimum pattern score".\par +\pard\plain \s4\qj\sa120\sl280 \f20 The search will start.\par +A typical run is shown in figure 13.6\par +\pard\plain \li1120\ri1280\sb200\sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth \f4\fs16 PIPL (Protein interpretation program (library)) V4.1 Jul 1991\par +\pard \li1120\ri1280\sl220\brdrl\brsp100\brdrth \brdrr\brsp100\brdrth Author\: Rodger Staden\par +Searches protein libraries for patterns of motifs\par +\par +? Name for results file=globin.res\par +Select a library\par + 1 EMBL nucleotide library \par +X 2 SWISSPROT protein library \par + 3 Personal file in PIR format \par + 4 Personal file in FASTA format \par +? Selection (1-4) (2) =\par +Library is in EMBL format with indexes\par +Select a task\par +X 1 Search whole library \par + 2 Search only a list of entries \par + 3 Search all but a list of entries \par +? Selection (1-3) (1) =\par +Select results display mode\par +X 1 Motif by motif \par + 2 Inclusive \par + 3 Scores only \par + 4 Complete padded sequences\par + 5 Padded sections \par +? Selection (1-5) (1) =5\par +? (y/n) (y) Report all matches n\par +? Pattern definition file=globin.pat\par + globin 1 \par +Probability of score -34.5300 = 0.197E-02\par + globin 2 \par +Probability of score -44.6000 = 0.409E-02\par + globin 3 \par +Probability of score -75.1000 = 0.293E-01\par + globin 4 \par +Probability of score -36.1000 = 0.147E-01\par + globin 5 \par +Probability of score -73.7000 = 0.375E-01\par + globin 6 \par +Probability of score -55.9000 = 0.483E-01\par +\par +Pattern description\par + Globin pattern file \par +Motif 1 named g1 is of class 4\par +Which is a match to a weight matrix with score -34.530\par +Motif 2 named g2 is of class 4\par +Which is a match to a weight matrix with score -44.600\par +and the N-terminal residue can take positions 17 to 22\par +relative to the N-terminal end of motif 1\par +It is anded with the previous motif.\par +Motif 3 named g3 is of class 4\par +Which is a match to a weight matrix with score -75.100\par +and the N-terminal residue can take positions 27 to 35\par +relative to the N-terminal end of motif 2\par +It is anded with the previous motif.\par +Motif 4 named g4 is of class 4\par +Which is a match to a weight matrix with score -36.100\par +and the N-terminal residue can take positions 29 to 53\par +relative to the N-terminal end of motif 3\par +It is anded with the previous motif.\par +Motif 5 named g5 is of class 4\par +Which is a match to a weight matrix with score -73.700\par +and the N-terminal residue can take positions 12 to 16\par +relative to the N-terminal end of motif 4\par +It is anded with the previous motif.\par +Motif 6 named g6 is of class 4\par +Which is a match to a weight matrix with score -55.900\par +and the N-terminal residue can take positions 29 to 33\par +relative to the N-terminal end of motif 5\par +It is anded with the previous motif.\par +Probability of finding pattern = 0.6273E-11\par +Expected number of matches per 1000 residues = 0.2119E-03\par +? Maximum pattern probability (0.00-1.00) (1.00) =\par +\pard \li1120\ri1280\sl220\keepn\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth ? Minimum pattern score (-9999.00-9999.00) (-9999.00) =\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa240\sl240\tx1140 \f21\fs20 Figure 13.6\tab A typical run of PIPL using a pattern of 6 weight matrices to search the SWISSPROT library.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.5\tab Preparing the PROSITE motif library for use by the programs\par +\pard\plain \s4\qj\sa120\sl280 \f20 Only the program SPLITP3 is essential for preparing the PROSITE library for use by our programs. \par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select SPLITP3\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Define "Prosite library file". Type the name of the file containing the prosite library (usually PROSITE.DAT).\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +Define "Name for file of pattern file names". This is the file of file names that users will employ to search the whole library. It will be convenient for them if an environment variable is defined for this file name.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Path name of motif directory". This is the full path name, including the final /, to the directory in which the converted library will be stored.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 3.\tab Notes\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab +The "exact match" motif class requires a consensus sequence. The "percentage match" motif class requires a consensus sequence and a cutoff score. The "score matrix" motif class uses the MDM78 matrix and requires a consensus sequence and a cutoff score. + The "weight matrix" search only requires the name of the file containing the matrix. The "direct repeat" motif class requires a repeat length, the minimum and maximum gap between the t +wo occurrences of the repeat, and a minimum score. The "membership of a set" motif class defines sets of residue types that are allowed at each position in the motif. When they are first entered into the pattern they are normally typed on the keyboard, but + when they are stored in a file, they are written in the same format as a weight matrix. To enter them on the keyboard use the following format. Type the one letter codes for the set of residue types allowed at each position terminated by a comma (,). For +positions where any residue type is allowed simply type an extra comma. For example VLI,FY,,,DE means any of Valine, Leucine or Isoleucine in the first position, either Phenylalanine or Tyrosine in the next position, anything in the next two positions, and + Aspartic acid or Glutamic acid in the next. When the pattern is stored on the disk the program will request a name for the file and a title for the motif.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab The details of the probabilty calculations are outside the scope of this article. They are quite +rapid and are essential both for assessing the statistical significance of any matches found and for allowing meaningful cutoffs to be applied to patterns. Obviously, in general, cutoff scores are inappropriate for patterns containing a mixture of motif cl +asses.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +The program calculates the "Probability of finding the pattern" and the "Expected number of matches". The first figure is actually the product of the individual motif probabilities but the latter figure is more useful because it takes into accoun +t the allowed variation in spacing between motifs and the length of the current sequence. In both cases the composition of the current sequence is also used so that different probabilities would be calculated for other sequences.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab +The pattern definition system is very flexible. Assume that a laboratory has a large library of patterns stored in its computer. Different groups or users may want to screen their sequences against different subsets of a pattern library. Each group ther +efore uses its own "File o +f pattern file names" which contains only the names of the pattern files that are relevant to their sequences. Of course a pattern may contain only one motif. Hence a library of patterns can include both simple and complex patterns. In the same way a labor +atory may have a large library of weight matrices defining different motifs and different users may want to combine them in different ways to produce their own patterns.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \tab Also, of course, a library does not have to be used solely for performing mass screenings\: + each individual entry can be used as a single pattern by giving the name of its pattern file - eg pathname/PS00002.PAT.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab +Note that 5 of the PROSITE motifs contains the symbols > or < which means that the motifs must appear exactly at the N or C termini of the sequences. Currently our methods have no mechanism for such definitions and, for example KDEL motifs, will be perm +itted to occur anywhere throughout a sequence.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 4.\tab References\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Staden, R. 1988. Methods to define and locate patterns of motifs in sequences. {\i CABIOS} {\b 4(1)}\:53-60.\par +2.\tab Staden, R. 1989. Methods for calculating the probabilities of finding patterns in sequences. {\i CABIOS} {\b 5(2)}\:89-96.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Staden, R. 1990. Searching for patterns in protein and nucleic acid sequences. (in) {\i Methods in Enzymology} R.F. Doolittle (ed.), {\b 183}\:193-211 (Academic Press, New York).\par +\pard\plain \s2\qc\sa200\sl480 \b\f20\fs36 \page 14. Comparing Sequences\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 Table of contents\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Introduction\par +2.\tab Methods\par +\pard \s7\qj\fi-560\li1700\sa120\sl280\tx1700 2.1\tab Producing a dot matrix plot (or list) of exact matches\par +2.2\tab Producing a dot matrix plot using the proportional algorithm\par +2.3\tab Producing a dot matrix plot using the quick scan algorithm\par +2.4\tab Producing a list of all matching segments using the proportional algorithm\par +2.5\tab Calculating the expected scores for the proportional algorithm\par +2.6\tab Calculating the observed scores for the proportional algorithm\par +2.7\tab Producing an optimal alignment\par +2.8\tab Comparing a sequence against a library of sequences\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab Notes\par +4.\tab References\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 1.\tab Introduction\par +\pard\plain \s4\qj\sa120\sl280 \f20 In this chapter we describe methods for comparing and aligning pairs of nucleic acid or protein +sequences. The program described (SIP), the original version of which was first described in 1982 (1), is based around several methods for producing "dot matrix" plots and includes routines for assessing the statistical significance of the plots, plus a d +ynamic programming algorithm for finding optimal alignments. At the end of the chapter we describe a program SIPL that is used for comparing a single sequence against a whole library of sequences.\par +\pard \s4\qj\sa120\sl280 We assume the reader is familiar with the general principl +e of dot matrix diagrams. The program uses a number of different algorithms to calculate the score for each point in a dot matrix and the user defines a minimum score so that only those points in the diagram for which the score is at least this value will +be marked with a dot. The first scoring method finds uninterrupted sections of perfect identity i.e. those that contain no mismatches, insertions or deletions. Generally this method, termed "the identities algorithm" is of limited value, but runs very qui +ckly. \par +\pard \s4\qj\sa120\sl280 +The second method looks for sections where a proportion of the characters in the sequence are similar, again allowing no insertions or deletions. For a thorough analysis this method, termed "the proportional algorithm", is the best. The original method, o +f this type was first described by McLachlan (2) and involves calculating a score for each position in the matrix by summing points found when looking forwards and backwards along a diagonal line of a given length (the window). The algorithm does no +t simply look for identity but uses a score matrix that contains scores for every possible pair of characters. For comparing amino acid sequences we usually use the score matrix MDM78 (3) which is shown in figure 14.1.. It is also possible to use other ma +trices, including an identity matrix for proteins. For nucleic acids we usually use an identity matrix.\par +\pard\plain \li220\ri280\sl220\box\brsp100\brdrth \f4\fs16 C S T P A G N D E Q B Z H R K M I L V F Y W - X ? \par +\pard \li220\ri280\sl220\box\brsp100\brdrth C 22 10 8 7 8 7 6 5 5 5 5 5 7 6 5 5 8 4 8 6 10 2 10 10 10 10\par +S 10 12 11 11 11 11 11 10 10 9 10 10 9 10 10 8 9 7 9 7 7 8 10 10 10 10\par +T 8 11 13 10 11 10 10 10 10 9 10 10 9 9 10 9 10 8 10 7 7 5 10 10 10 10\par +P 7 11 10 16 11 9 9 9 9 10 9 10 10 10 9 8 8 7 9 5 5 4 10 10 10 10\par +A 8 11 11 11 12 11 10 10 10 10 10 10 9 8 9 9 9 8 10 6 7 4 10 10 10 10\par +G 7 11 10 9 11 15 10 11 10 9 10 10 8 7 8 7 7 6 9 5 5 3 10 10 10 10\par +N 6 11 10 9 10 10 12 12 11 11 12 11 12 10 11 8 8 7 8 6 8 6 10 10 10 10\par +D 5 10 10 9 10 11 12 14 13 12 13 12 11 9 10 7 8 6 8 4 6 3 10 10 10 10\par +E 5 10 10 9 10 10 11 13 14 12 12 13 11 9 10 8 8 7 8 5 6 3 10 10 10 10\par +Q 5 9 9 10 10 9 11 12 12 14 11 13 13 11 11 9 8 8 8 5 6 5 10 10 10 10\par +B 5 10 10 9 10 10 12 13 12 11 13 11 11 10 10 8 8 6 8 5 7 4 10 10 10 10\par +Z 5 10 10 10 10 10 11 12 13 13 11 14 12 10 10 8 8 8 8 5 6 4 10 10 10 10\par +H 7 9 9 10 9 8 12 11 11 13 11 12 16 12 10 8 8 8 8 8 10 7 10 10 10 10\par +R 6 10 9 10 8 7 10 9 9 11 10 10 12 16 13 10 8 7 8 6 6 12 10 10 10 10\par +K 5 10 10 9 9 8 11 10 10 11 10 10 10 13 15 10 8 7 8 5 6 7 10 10 10 10\par +M 5 8 9 8 9 7 8 7 8 9 8 8 8 10 10 16 12 14 12 10 8 6 10 10 10 10\par +I 8 9 10 8 9 7 8 8 8 8 8 8 8 8 8 12 15 12 14 11 9 5 10 10 10 10\par +L 4 7 8 7 8 6 7 6 7 8 6 8 8 7 7 14 12 16 12 12 9 8 10 10 10 10\par +V 8 9 10 9 10 9 8 8 8 8 8 8 8 8 8 12 14 12 14 9 8 4 10 10 10 10\par +F 6 7 7 5 6 5 6 4 5 5 5 5 8 6 5 10 11 12 9 19 17 10 10 10 10 10\par +Y 10 7 7 5 7 5 8 6 6 6 7 6 10 6 6 8 9 9 8 17 20 10 10 10 10 10\par +W 2 8 5 4 4 3 6 3 3 5 4 4 7 12 7 6 5 8 4 10 10 27 10 10 10 10\par +- 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10\par +X 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10\par +? 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10\par +\pard \li220\ri280\sl220\keepn\box\brsp100\brdrth 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa400\sl240\tx1140 \f21\fs20 Figure 14.1\tab The amino acid score matrix MDM78.\par +\pard\plain \s4\qj\sa120\sl280 \f20 +For the proportional method plotting dots at the centres of windows that reach the cutoff leads to a persistence effect that, to some extent, can be mitigated by a variation on the method. If, for example, all the high scoring amino acids are clustered at +the left end of a particular diagonal segment, dots will continue to be plotted to their right until the window score drops below the cutoff. Instead of plotting a single point for each window that reaches the cutoff score, the variant method plots p +oints for all the identities that lie in windows that reach the cutoff. Obviously the persistence effect can be more pronounced for long windows and low cutoff scores, but note that the variant method will plot nothing if there are no identities present, a +nd so similar regions could be missed! A further variant, useful for comparing a sequence against itself, ignores the main diagonal.\par +\pard \s4\qj\sa120\sl280 The third comparison method called "quick scan" is really a combination of the first two, and is similar to the FASTP prog +ram of Lipman and Pearson (4), but produces a dot matrix diagram. The algorithm is as follows. The dot matrix positions are found for all words of some minimum length (obviously length 1 is most sensitive) that are common to both sequences. Imagine a diago +nal line running from corner to corner of the diagram, at right angles to the diagonals in the dot matrix, The scores for the common words (according to the current score matrix, e.g. MDM78) are accummulated at the appropriate positions on that imaginary l +ine, hence producing a histogram. The histogram is analysed to find its mean and standard deviation. The diagonals that lie above some cutoff score (defined in standard deviation units), are rescanned using the proportional algorithm, and a diagram produce +d. The method is very fast, and is also employed by the library comparison program (see below).\par +\pard \s4\qj\sa120\sl280 \par +\pard \s4\qj\sa120\sl280 The dynamic programming alignment algorithm contained in the program is based on that of Myers and Miller (5). It guarantees to produce alignments with the opt +imum score given a score matrix, a gap start penalty, and a gap extension penalty. It is very useful to have the dot matrix methods and the alignment routine together in the same program because it allows users to produce a dot matrix diagram to help selec +t which regions of the sequence they wish to align. Selection is made by use of the crosshair. The crosshair is positioned first at the bottom left hand end of the segment to be aligned and then at the top right of the segment. When the alignment routine i +s selected the segment will be aligned. The alignment can replace the original segment of the sequence. By repeated plotting of dot matrices, followed by alignment, very long sequences can easily be aligned. \par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 2.\tab Methods\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.1\tab Producing a dot matrix plot (or list) of exact matches\par +\pard\plain \s4\qj\sa120\sl280 \f20 This method is relatively fast and can be useful for very similar sequences. It marks the position of every exact match of some minimum length with a dot or lists out the matching segments.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Apply identities algorithm".\par +2.\tab Define "Identity score". \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3.\tab +Select "Plot or List". The plot will appear as in figure 14.2, which shows a comparison of two protein sequences using a score of 2. 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+021008110320000080f7000028fe000040f7000008140020fd00018001f60000a0fe000040fc000102080c0020fa000021ef0002200008110020fd000302000010fd000001f2000008140620000080000088fa000028fe000040f70000080a0020e9000004fe00000812012020f7000080fb000302000040fb0000080b0520 +8010000008ea000008120020fd000411c8000020fb000020f5000008120020fc000010fb000080f8000001fc000028140020fc0005020002008004fb000010f800010848160020fe00044002000010fd000001fa000004fa0000080e0020fd000004f4000004f8000008120020fd0002040001f700014080f9000110081100 +20fc000304000040f9000001f70000081605200000010004fb000030fd000340000010f9000008120020fe000002f7000040fa000004fc000008140020fd000040f7000304002020fb0003200000081302200004fe00018080f200010240fe00010108150020fd000001fe000010fd000008f60003800000080a0020f20000 +80f50000081102200044fe00018220f5000040f90000080c02200040ed000004fc0000080d0020f0000008fa0003040000080e0020fe000008f8000040f3000008160320810004fe000004fe00010202f8000004fb0000080a0020f6000040f100000813012001f800018002fa0002012008fc000102080c0020ed00010208 +fc000140080e0020fc000088f0000040fd0000080a0020e9000008fe0000080e0020fa000008f1000008fe0000081605200000010004fb000020fd000340400010f90000080a0020e9000004fe000008160020fe000080fe000080f90000a0fa000001fe000008090320040010e8000008060020e50000080a0020fa000008 +ed0000081605200000010004fb000020fd000340400010f90000081c0320000002fb00042800081086fe000002fe00010202fe00028000080a0020f3000080f4000008120020fc000040f7000010fe000004fa0000080e0020fd000004f4000004f80000081b0020fe000620000008020008fe00010410fc0002400002fc00 +00080a0020f2000008f5000008060020e5000008140020fb000008f9000304020020fb0003300000081408202000001000200021fe000081f30002200008190020fe00042000080802fe000308000010fc000042fa0000080f0020f90002220008f30003040000081002200020fd000001f6000001f80000080f012001fb00 +0008fe000002f1000008190020fc000002fe000080fa000010fe000080fe0003080008081b0020fd0008100000040080000080fd000008fd000001fd0001802806003fe5ff00f80000ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb80\sa400\sl240\tx1140 \f21\fs20 Figure 14.2\tab A dot-matrix for two related protein sequences using the "Identities algorithm" and a score of 2. Notice that the similarity is not apparent. \par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.2\tab Producing a dot matrix plot using the proportional algorithm\par +\pard\plain \s4\qj\sa120\sl280 \f20 This method gives the most thorough analysis.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Apply proportional algorithm".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Define "Odd window length". The size of window over which the scores for each point are summed.\par +3.\tab Define "Proportional score". All points achieving at least this score will be marked with a dot in the diagram.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The plot will appear as in figure 14.3.\par +\pard\plain \qj\li1700\sb300\sl480\keepn \f4\fs16 {{\pict\macpict\picw283\pich301 +08a200000000012d011b001102ff0c00fffe0000003c32b0003c32b00000000000fc00ed000000000001000a0000000000fc00ed0098801e0000000000fc00ed0000000000000000003c32b0003c32b000000001000100010000000000000000000000000048ae57000000010000ffffffffffff0001000000000000000000 +0000fc00ed0000000000fc00ed000002e30006007fe5ff00f0060040e5000010060040e5000010060040e5000010060040e5000010060040e50000100b0040f200010180f60000100a0040f2000003f50000100a0040f2000006f50000100a0040f2000004f50000100d0340000020f5000008f5000010090340000020e800 +0010060040e5000010090340000080e80000100802400001e70000100802400003e7000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e50000 +10060040e5000010060040e5000010060040e5000010060040e5000010060040e50000100a0040f2000060f50000100a0040f2000040f5000010060040e50000100a0040ea000040fd0000100c0040ec0002040080fd000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040 +e5000010060040e5000010060040e5000010060040e5000010060040e50000100a0040eb000080fc0000100a0040ec000001fb0000100a0040ec000002fb000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e50000 +10060040e50000100a0040f9000010ee0000100a0040f9000030ee0000100a0040f9000060ee0000100a0040f90000c0ee0000100e0040f9000080fc000020f40000100a0040eb000040fc000010060040e5000010060040e5000010060040e5000010060040e50000100a0040ee000004f9000010060040e5000010060040 +e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e50000100a0040f0000002f7000010060040e5000010 +060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010070040e600018010060040e5000010060040e50000100b0040fd000101 +80eb000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e50000100a0040f4000002f30000100a0040f4000006f30000100a0040f400000cf30000100a0040f4000008f30000100a0040f4000010f30000100a0040f4 +000030f30000100a0040f4000060f30000100a0040f4000040f3000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010090040e8000301000010060040e5 +000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e50000100a0040f7000004f00000100a0040f7000004f0000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5 +000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e50000100a0040f9000008ee000010060040e5000010060040e50000100a0040f9000020ee0000100a0040f9 +000040ee0000100a0040f9000080ee0000100a0040fa000001ed0000100a0040fa000002ed0000100a0040fa000004ed0000100a0040fa000004ed0000100a0040fa000008ed000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e500 +0010060040e5000010060040e5000010060040e5000010060040e50000100a0040fb000040ec0000100a0040fb000080ec0000100a0040fc000001eb0000100a0040fc000002eb0000100a0040fc000006eb0000100a0040fc000004eb0000100a0040fc000008eb0000100a0040fc000010eb0000100a0040fc000020eb00 +00100a0040fc000060eb0000100a0040fc000080eb0000100b0040fd00010180eb0000100a0040fd000001ea0000100a0040fd000002ea0000100a0040fd000004ea0000100a0040fd000008ea0000100a0040fd000008ea0000100a0040fd000010ea0000100a0040fd000020ea0000100a0040fd000040ea000010060040 +e50000100a0040fe000001e9000010060040e50000100a0040fe000002e90000100a0040fe000004e90000100a0040fe000008e9000010060040e50000100e0040fe000010f0000040fb000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e500001006 +0040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e50000100d0040fd000303000020ed000010060040e5000010060040e5000010060040e50000100a0040fc00000ceb0000100a0040fc00 +0008eb000010060040e5000010060040e5000010060040e5000010060040e5000010060040e500001006007fe5ff00f00000ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa240\sl240\tx1140 \f21\fs20 Figure 14.3\tab +A dot-matrix for the two related protein sequences shown in figure 14.2, but here using the "Proportional algorithm" with a window of 21 and a score of 240. Notice that the similarity is now apparent. \par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.3\tab Producing a dot matrix plot using the quick scan algorithm\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This method is very fast. Using the current score matrix it accumulates the scores for all the exact matches that lie on each diagonal. The mean diagonal score and its standard deviation is calculated, and those diagonals that have scores more than a chose +n number of standard deviations above the mean are rescanned using the proportional algorithm and the points above the proportional algorithms cutoff are plotted.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Apply quick scan algorithm".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Define "Identity score". The minimum number of consecutive identical sequence symbols that count as a match.\par +3.\tab Define "Odd window length". The size of window over which the scores for each point are summed when the proportional algorithm is applied to the best diagonals.\par +4.\tab Define "Proportional score". For the best diagonals all points achieving at least this score will be marked with a dot in the diagram.\par +5.\tab Define "Number of s.d. above mean". Diagonals with scores above the minimum number of standard deviations are rescanned using the proportional algorithm.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The plot will appear as in figure 14.4.\par +\pard\plain \qj\li1720\sb300\sl480\keepn \f4\fs16 {{\pict\macpict\picw283\pich301 +07fa00000000012d011b001102ff0c00fffe0000003c32b0003c32b00000000000fc00ed000000000001000a0000000000fc00ed0098801e0000000000fc00ed0000000000000000003c32b0003c32b0000000010001000100000000000000000000000000491cbd000000010000ffffffffffff0001000000000000000000 +0000fc00ed0000000000fc00ed000002e30006007fe5ff00f0060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5 +000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e500 +0010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e50000 +10060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010 +060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e500001006 +0040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e50000100600 +40e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040 +e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5 +000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e500 +0010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e50000100a0040f9000008ee000010060040e5000010060040e50000100a0040f9000020ee0000100a0040f9000040ee0000100a0040f9000080ee0000100a0040fa +000001ed0000100a0040fa000002ed0000100a0040fa000004ed0000100a0040fa000004ed0000100a0040fa000008ed000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e50000 +10060040e50000100a0040fb000040ec0000100a0040fb000080ec0000100a0040fc000001eb0000100a0040fc000002eb0000100a0040fc000006eb0000100a0040fc000004eb0000100a0040fc000008eb0000100a0040fc000010eb0000100a0040fc000020eb0000100a0040fc000060eb0000100a0040fc000080eb00 +00100b0040fd00010180eb0000100a0040fd000001ea0000100a0040fd000002ea0000100a0040fd000004ea0000100a0040fd000008ea0000100a0040fd000008ea0000100a0040fd000010ea0000100a0040fd000020ea0000100a0040fd000040ea000010060040e50000100a0040fe000001e9000010060040e5000010 +0a0040fe000002e90000100a0040fe000004e90000100a0040fe000008e9000010060040e50000100a0040fe000010e9000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e50000 +10060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010060040e5000010 +06007fe5ff00f00000ff}}\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa240\sl240\tx1140 \f21\fs20 Figure 14.4\tab +A dot-matrix for the two related protein sequences shown in figures 14.2 and 14.3, but here using the "Quick scan algorithm" with an identity score of 1 and a window of 21 and a score of 240 for the proportional algorithm. Notice that the simil +arity is now apparent but the absence of background "noise" is misleading.\par +\pard\plain \s6\fi-540\li560\sb240\sa60\sl280\tx860 \b\f20 2.4\tab Producing a list of all matching segments using the proportional algorithm\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "List matching segments".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Define "Odd window length". The size of window over which the scores for each point are summed.\par +3.\tab Define "Proportional score". All segments achieving at least this score will be listed out with the two sequences written one above the other. See figure 14.5.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.5\tab Calculating the expected scores for the proportional algorithm\par +\pard\plain \s4\qj\sa120\sl280 \f20 This function calculates the probability of achieving each possible score using the proportional algorithm. Hence it provides a method of setting + cutoff scores and assessing the statistical significance of the scores found. The algorithm calculates the "Double matching probability" described by McLachlan (2) which is defined as the probability of finding the scores in two infinitely long sequences +of the same composition as the pair being compared. It is very much faster than the alternative of repeatedly scrambling and recomparing the sequences. The program offers three ways for the user to see the results of the calculation\: + the user can type a \par +\pard\plain \li2320\ri2720\sl220\box\brsp100\brdrth \f4\fs16 List matching segments\par +\pard \li2320\ri2720\sl220\box\brsp100\brdrth ? Odd window length (1-401) (11) =\par +? Proportional score (1-567) (252) =\par +Working\par + 62\par +GLRRGLDVKDLEHPIEVPVGK\par +DLAEGMKVKCTGRILEVPVGR\par + 81\par + 63\par +LRRGLDVKDLEHPIEVPVGKA\par +LAEGMKVKCTGRILEVPVGRG\par + 82\par + 65\par +RGLDVKDLEHPIEVPVGKATL\par +EGMKVKCTGRILEVPVGRGLL\par + 84\par + 66\par +GLDVKDLEHPIEVPVGKATLG\par +GMKVKCTGRILEVPVGRGLLG\par + 85\par + 67\par +LDVKDLEHPIEVPVGKATLGR\par +MKVKCTGRILEVPVGRGLLGR\par +\pard \li2320\ri2720\sl220\keepn\box\brsp100\brdrth 86\par +\pard\plain \s8\qj\fi-1140\li1140\sb60\sa400\sl240\tx1140 \f21\fs20 Figure 14.5\tab A typical run of "List matching segments.\par +\pard\plain \s4\qj\sa120\sl280 \f20 score and the program will display its probability; the user can type a probability and the program will display the corresponding score, alternatively the program will list the full range of scores and probabilities. +\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Calculate expected scores".\par +2.\tab Define "Odd window length".\par +\tab The calculation takes a noticeable time.\par +3.\tab Select "List scores and probabilities".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Number of steps between scores". This allows, say, every fifth score to be listed if the user defines the number of steps to be 5. The list will appear as in figure 14.6.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.6\tab Calculating the observed scores for the proportional algorithm\par +\pard\plain \s4\qj\sa120\sl280 \f20 +This function applies the proportional algorithm, but instead of producing a dot matrix it accumulates the scores and their frequencies of occurrence. It provides a method of setting cutoff scores and assessing the statistical significance of the scores fo +und. The program offers three ways for the user to see the results of the calculation\: the user can type a score and the program will display its frequency; the user can type a frequency and the progra +m will display the corresponding score, alternatively the program will list the full range of scores and frequencies. The frequencies are expressed as percentages.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Calculate observed scores".\par +2.\tab Define "Odd window length".\par +\tab The calculation takes a noticeable time.\par +\pard\plain \li1320\ri1300\sl220\box\brsp100\brdrth \f4\fs16 Calculate expected proportional scores\par +\pard \li1320\ri1300\sl220\box\brsp100\brdrth ? Odd window length (1-401) (21) =\par +Working\par +Average score= 196.99062\par +Select probability display mode\par + 1 Show probability for a score\par +X 2 Show score for a probability\par + 3 List scores and probabilities\par +? Selection (1-3) (2) =3\par +? Number of steps between scores (1-10) (5) =\par +\par + 5 0.10000E+01 200 0.40004E+00 395 0.00000E+00\par + 10 0.10000E+01 205 0.24037E+00 400 0.00000E+00\par + 15 0.10000E+01 210 0.12555E+00 405 0.00000E+00\par + 20 0.10000E+01 215 0.56905E-01 410 0.00000E+00\par + 25 0.10000E+01 220 0.22402E-01 415 0.00000E+00\par + 30 0.10000E+01 225 0.76821E-02 420 0.00000E+00\par + 35 0.10000E+01 230 0.23031E-02 425 0.00000E+00\par + 40 0.10000E+01 235 0.60614E-03 430 0.00000E+00\par + 45 0.10000E+01 240 0.14064E-03 435 0.00000E+00\par + 50 0.10000E+01 245 0.28888E-04 440 0.00000E+00\par + 55 0.10000E+01 250 0.52741E-05 445 0.00000E+00\par + 60 0.10000E+01 255 0.85917E-06 450 0.00000E+00\par + 65 0.10000E+01 260 0.12534E-06 455 0.00000E+00\par + 70 0.10000E+01 265 0.16433E-07 460 0.00000E+00\par + 75 0.10000E+01 270 0.19425E-08 465 0.00000E+00\par + 80 0.10000E+01 275 0.20772E-09 470 0.00000E+00\par + 85 0.10000E+01 280 0.20155E-10 475 0.00000E+00\par + 90 0.10000E+01 285 0.17801E-11 480 0.00000E+00\par + 95 0.10000E+01 290 0.14353E-12 485 0.00000E+00\par + 100 0.10000E+01 295 0.10599E-13 490 0.00000E+00\par + 105 0.10000E+01 300 0.71886E-15 495 0.00000E+00\par + 110 0.10000E+01 305 0.44920E-16 500 0.00000E+00\par + 115 0.10000E+01 310 0.25938E-17 505 0.00000E+00\par +\pard \li1320\ri1300\sl220\keepn\box\brsp100\brdrth 120 0.10000E+01 315 0.13881E-18 510 0.00000E+00\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa500\sl240\tx1140 \f21\fs20 Figure 14.6\tab A typical run of "Calculate expected proportional scores." The scores are listed in three columns alongside their probabilities. e.g. score 250 has a probability 0.527x10 +{\up6 -5}{\plain \b\f20 .}{\up6 \par +}\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 3.\tab Select "List scores and percentages".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Number of steps between scores". This allows, say, every fifth score to be listed if the user defines the number of steps to be 5. The list will appear as in figure 14.7.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.7\tab Producing an optimal alignment\par +\pard\plain \s7\qj\sa120\sl280\tx0 \f20 This function produces an optimal alignment for any segments of the two sequences +using the algorithm of Myers and Miller (5). It guarantees to produce alignments with the optimum score, given a score matrix, a "gap start penalty" and a "gap extension penalty". That is starting a gap costs a fixed penalty F and each residue added to the + gap costs a further penalty E, so for \par +\pard\plain \li1980\ri2060\sb400\sl220\box\brsp100\brdrth \f4\fs16 Calculate observed proportional scores\par +\pard \li1980\ri2060\sl220\box\brsp100\brdrth ? Odd window length (1-401) (21) =\par +Working\par +Maximum observed score is 285\par +Select score display mode\par +X 1 Show percentage reaching a score\par + 2 Show score for a percentage\par + 3 List scores and percentages\par +? Selection (1-3) (1) =3\par + ? Number of steps between scores (1-10) (5) =\par + 156 236949 0.99998E+02\par + 161 236938 0.99993E+02\par + 166 236792 0.99932E+02\par + 171 235882 0.99548E+02\par + 176 232582 0.98155E+02\par + 181 222875 0.94058E+02\par + 186 203232 0.85769E+02\par + 191 171507 0.72380E+02\par + 196 131216 0.55376E+02\par + 201 89194 0.37642E+02\par + 206 52791 0.22279E+02\par + 211 27315 0.11528E+02\par + 216 12117 0.51137E+01\par + 221 4890 0.20637E+01\par + 226 1774 0.74867E+00\par + 231 656 0.27685E+00\par + 236 263 0.11099E+00\par + 241 111 0.46845E-01\par + 246 66 0.27854E-01\par + 251 36 0.15193E-01\par + 256 23 0.97065E-02\par + 261 16 0.67524E-02\par + 266 15 0.63303E-02\par + 271 10 0.42202E-02\par + 276 6 0.25321E-02\par +\pard \li1980\ri2060\sl220\box\brsp100\brdrth 281 2 0.84405E-03\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa400\sl240\tx1140 \f21\fs20 Figure 14.7\tab +A typical run of "Calculate observed scores." The scores are followed by their observed number of occurrences expressed both absolutely and as a percentage of the total number of points.\par +\pard\plain \s4\qj\sa120\sl280 \f20 +gap of length K residues the penalty is F + KE. Gaps at the ends of sequences incur no penalty. The size of the segments of sequence that can be aligned at once is limited to 5000 characters. The user can select the start and end of the segments by use of +the crosshair simply by clicking on any dot matrix plot. After the alignment has been produce the user can elect to have it replace the original sequence segments. By alternate use of dot matrix plotting and alignment, very long sequences can be aligned. +\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select "Align sequences". The crosshair will appear in the graphics window. \par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 2.\tab Position the crosshair on the bottom left of the segment to be aligned and hit the space bar on the keyboard. The bell will ring.\par +3.\tab Position the crosshair on the top right of the segment to be aligned and hit the space bar on the keyboard. The bell will ring.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab Define "Penalty for starting each gap".\par +5.\tab Define "penalty for each residue in gap".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 \tab A noticeable time will elapse before the alignment is displayed on the screen. A typical alignment is shown in figure 14.8.\par +6.\tab Reject "Keep alignment". If the alignment is "kept" the padded sequences from the alignment will replace the original sequences in the active region.\par +\pard\plain \li480\ri540\sl220\box\brdrth \f4\fs16 Align the sequences\par +\pard \li480\ri540\sl220\box\brdrth Aligning region 1 to 461\par + with region 1 to 514\tab \tab Working\par + V 1 11 21 31 41 51\par + MA--TGKIVQ VIGA------ VVDVEFPQDA VPRVYDALEV QNG------N ERLVL-----\par + * * * ** * * * * *\par + MQLNSTEISE LIKQRIAQFN VVSEAHNEGT IVSVSDGVIR IHGLADCMQG EMISLPGNRY\par + H 1 11 21 31 41 51\par + V 61 71 81 91 101 111\par + EVQQQLGGGI VRTIAMGSSD GLRRGLDVKD LEHPIEVPVG KATLGRIMNV LGEPVDMKGE\par + * * ** * * ** ***** *** * ** * * **\par + AIALNLERDS VGAVVMGPYA DLAEGMKVKC TGRILEVPVG RGLLGRVVNT LGAPIDGKGP\par + H 61 71 81 91 101 111\par + V 121 131 141 151 161 171\par + IGEEERWAIH RAAPSYEELS NSQELLETGI KVIDLMCPFA KGGKVGLFGG AGVGKTVNMM\par + * ** * ** * * * * * * ***\par + LDHDGFSAVE AIAPGVIERQ SVDQPVQTGY KAVDSMIPIG RGQRELIIGD RQTGKTALAI\par + H 121 131 141 151 161 171\par + V 181 191 201 211 221 231\par + ELIRNIAIEH SGYS-VFAGV GERTREGNDF YHEMTDSNVI DKVSLVYGQM NEPPGNRLRV\par + * * ** * * *\par + DAI--INQRD SGIKCIYVAI GQKASTISNV VRKLEEHGAL ANTIVVVATA SESAALQYLA\par + H 181 191 201 211 221 231\par + V 241 251 261 271 281 291\par + ALTGLTMAEK FRDEGRDVLL FVDNIYRYTL AGTEVSALLG RMPSAVGYQP TLAEEMGVLQ\par + * * *** * * * * * * ** * * *\par + RMPVALMGEY FRDRGEDALI IYDDLSKQAV AYRQISLLLR RPPGREAFPG DVFYLHSRLL\par + H 241 251 261 271 281 291\par + V 301 311 321 331 341 351\par + ERITST---- ---------- -KTGSITSVQ AVYVPADDLT DPSPATTFAH LDATVVLSRQ\par + ** **** * * * * * *\par + ERAARVNAEY VEAFTKGEVK GKTGSLTALP IIETQAGDVS AFVPTNVISI TDGQIFLETN\par + H 301 311 321 331 341 351\par + V 361 371 381 391 401 411\par + IASLGIYPAV DPLDSTSRQL DPLVVGQEHY DTAR----GV QSILQRYQEL KDIIAILGMD\par + ** *** * * ** * * * * * **\par + LFNAGIRPAV NPGISVSR-- ---VGGAAQT KIMKKLSGGI RTALAQYREL AAFSQFAS--\par + H 361 371 381 391 401 411\par + V 421 431 441 451 461 471\par + ELSEEDKLVV ARARKIQRFL SQ----PFFV AE----VFTG SPGKYVSLKD --TIRGFKGI\par + * * * * * * * * * * * *\par + DLDDATRKQL DHGQKVTELL KQKQYAPMSV AQQSLVLFAA ERG-YLADVE LSKIGSFEAA\par + H 421 431 441 451 461 471\par + V 481 491 501 511 521\par + MEG--EYDHL P-EQAFYMVG SIEEAVE--- --------KA KKL*\par + ** * * * * *\par + LLAYVDRDHA PLMQEINQTG GYNDEIEGKL KGILDSFKAT QSW*\par + H 481 491 501 511 521\par +Conservation 22.5%\par +\pard \li480\ri540\sl220\keepn\box\brdrth Number of padding characters inserted 63 and 10\par +\pard\plain \s8\qj\fi-1140\li1140\sb60\sa300\sl240\tx1140 \f21\fs20 Figure 14.8\tab A typical output from "Align the sequences". The horizontal and vertical sequences are labelled H and V.\par +\pard\plain \s6\sb240\sa60\sl280\tx560\tx860 \b\f20 2.8\tab Comparing a sequence against a library of sequences\par +\pard\plain \s4\qj\sa120\sl280 \f20 +The program SIPL is used for comparing a probe sequence against a whole library of sequences. The searches are very fast and use the "Quick scan" algorithm described above to produce a list of matching sequences sorted in score order, and optionally, this +is followed by the production of optimal alignments using the Myers and Miller (5) algorithm. The program will search the whole of a library or restrict its search using a list of entry names. The list of + entry names can be used either as a list of sequences to search or conversely as a list of sequences to exclude from a search.\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab Select SIPL.\par +2.\tab Select "Personal file".\par +3.\tab Select "Format".\par +4.\tab Define "Name of sequence file". The name of the file containing the probe sequence.\par +5.\tab Define "Name of results file".\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 6.\tab Accept "Display alignments". The alternative will stop after producing a list of the best matching sequences.\par +7.\tab Define "Minimum library sequence length". This permits the search to skip sequences that are too short to be of interest.\par +8.\tab Define "Maximum number of scores to list". The maximum number of sequences that will be included in the results file.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 9.\tab +Define "Identity score". This is the minimum number of consecutive sequence characters that will be counted as a match. Only matches of at least this length will be included in the overall score. For proteins maximum sensitivity is gained using a value +of 1, but for nucleic acids values of 4 or 6 are necessary to achieve reasonable speed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 10.\tab Define "Number of sd above mean". This means the number of standard deviations above the mean that a diagonal must score in order for it to be scanned using the proportional algorithm.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 11.\tab Define "Odd window length". This is the window size for the rescanning of high scoring diagonals using the proportional algorithm.\par +12.\tab Define "Proportional score". The score used by the proportional algorithm. It depends on the window length and the score matrix.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 13.\tab Define "Minimum global score". This is the total score achieved using the proportional algorithm when all the diagonals scoring the defined number of standard deviations above the mean, are rescanned. +\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 14.\tab Define "Penalty for starting a gap". This is for the alignment algorithm.\par +15.\tab Define "Penalty for each residue in gap". See above.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 16.\tab Select a library to search. The default library will reflect the composition of the probe sequence. That is, a probe sequence that is less than 85% acgt will be guessed to be a protein.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 17.\tab Select "Search whole library". The alternatives allow the search to be restricted using a list of entry names.\par +\pard\plain \s4\qj\sa120\sl280 \f20 The search will start. A large number of parameters are required but for normal use the default value can be taken for them all. A worked example is shown in figure 14.9.\par +\pard\plain \li220\ri240\sl220\brdrt\brsp100\brdrth \brdrl\brsp100\brdrth \brdrr\brsp100\brdrth \f4\fs16 SIPL (Similarity investigation program (Library)) V3.0 June 1991\par +\pard \li220\ri240\sl220\brdrl\brsp100\brdrth \brdrr\brsp100\brdrth Author\: Rodger Staden\par + Compares a probe protein or nucleic acid\par + sequence against a library of sequences\par +\par +Select probe sequence\par + Select sequence source\par + X 1 Personal file \par + 2 Sequence library\par + ? Selection (1-2) (1) =2\par + Select a library\par + 1 EMBL nucleotide library \par + X 2 SWISSPROT protein library\par + 3 PIR protein library \par + ? Selection (1-3) (2) =\par +Library is in EMBL format with indexes\par + Select a task\par + X 1 Get a sequence \par + 2 Get annotations \par + 3 Get entry names from accession numbers \par + 4 Search titles for keywords \par + 5 Search keyword index for keywords \par + ? Selection (1-5) (1) =\par + ? Entry name=bacr$halha\par +DE BACTERIORHODOPSIN PRECURSOR (BR) (GENE NAME\: BOP). \par + Sequence length= 262\par + Sequence composition\par + A C S T P A G N D E Q B Z H\par + N 0. 14. 19. 12. 30. 26. 3. 10. 11. 4. 0. 0. 0.\par + % 0.0 5.3 7.3 4.6 11.5 9.9 1.1 3.8 4.2 1.5 0.0 0.0 0.0\par + W 0. 1219. 1921. 1165. 2132. 1483. 342. 1151. 1420. 513. 0. 0. 0.\par +\par +A R K M I L V F Y W - X ? \par +N 7. 7. 10. 15. 39. 23. 13. 11. 8. 0. 0. 0. 0.\par +% 2.7 2.7 3.8 5.7 14.9 8.8 5.0 4.2 3.1 0.0 0.0 0.0 0.0\par +W 1093. 897. 1312. 1697. 4413. 2280. 1913. 1795. 1490. 0. 0. 0. 0.\par +Total molecular weight= 28256.254\par +? Results file=sipl.res\par +? Display alignments (y/n) (y) =\par +? Minimum library sequence length (10-20000) (209) =\par +? Maximum number of scores to list (1-10000) (20) =10\par +? Identity score (1-3) (1) =\par +? Number of sd above mean (0.00-10.00) (3.00) =\par +? Odd window length (1-31) (11) =\par +? Proportional score (1-297) (132) =\par +? Minimum global score (1-69168) (1729) =\par +? Penalty for starting a gap (1-100) (10) =\par +? Penalty for each residue in gap (1-100) (10) =\par +Select a library\par + 1 EMBL nucleotide library \par +X 2 SWISSPROT protein library\par + 3 PIR protein library \par + 4 Personal file in PIR format \par +? Selection (1-4) (2) =\par +Library is in EMBL format with indexes\par +Select a task\par +X 1 Search whole library \par + 2 Search only a list of entries \par + 3 Search all but a list of entries \par +? Selection (1-3) (1) =3\par +? File of entry names=skip.nam\par + 21794 entries processed, 25 above cutoff, sorting now\par +Entries exceeding sd cutoff= 4439\par +Mean number of diagonals above span cutoff 1.32012\par +List in score order\par + 31007 BACA$HALSA DE ARCHAERHODOPSIN PRECURSOR (AR). \par + 12177 BACH$NATPH DE HALORHODOPSIN PRECURSOR (HR) (GENE NAME\: HOP). \par + 10999 BACH$HALSP DE HALORHODOPSIN PRECURSOR (HR) (GENE NAME\: HOP). \par + 3999 HYAC$ECOLI DE HYPOTHETICAL 27.6 KD PROTEIN IN HYAB 3'REGION (GENE NAM\par + 2670 OPS4$DROME DE OPSIN RH4 (INNER R7 PHOTORECEPTOR CELLS OPSIN) (GENE NA\par + 2573 PYR1$MESAU DE CAD PROTEIN (CONTAINS\: GLUTAMINE-DEPENDENT CARBAMOYL-PH\par + 2328 PFLA$ECOLI DE PYRUVATE FORMATE-LYASE ACTIVATING ENZYME. \par + 2194 DCOP$CANAL DE OROTIDINE 5'-PHOSPHATE DECARBOXYLASE (EC 4.1.1.23) (OMP\par + 2145 BCM1$HUMAN DE LYMPHOCYTE ACTIVATION MARKER BLAST-1 PRECURSOR (BCM1 SU\par + 2103 LAG3$HUMAN DE LAG-3 PROTEIN PRECURSOR (FDC PROTEIN) (GENE NAME\: LAG3 \par + BACA$HALSA DE ARCHAERHODOPSIN PRECURSOR (AR). \par + V 1 11 21 31 41 51\par + MLELLPTAVE GVSQAQITGR PEWIWLALGT ALMGLGTLYF LVKGMGVSDP DAKKFYAITT\par + * ** ** ** ** ** ** ** *** ** * * * ** \par + M-DPIALTAA VGADLLGDGR PETLWLGIGT LLMLIGTFYF IVKGWGVTDK EAREYYSITI\par + H 1 11 21 31 41 51\par + V 61 71 81 91 101 111\par + LVPAIAFTMY LSMLLGYGLT MVPFGGEQNP IYWARYADWL FTTPLLLLDL ALLVDADQGT\par + *** ** * *** * *** * * * ** ******* ********** *** * \par + LVPGIASAAY LSMFFGIGLT EVQVGSEMLD IYYARYADWL FTTPLLLLDL ALLAKVDRVS\par + H 61 71 81 91 101 111\par + V 121 131 141 151 161 171\par + ILALVGADGI MIGTGLVGAL TKVYSYRFVW WAISTAAMLY ILYVLFFGFT SKAESMRPEV\par + * *** * ** ******* * * * ** * ** * * ***\par + IGTLVGVDAL MIVTGLVGAL SHTPLARYTW WLFSTICMIV VLYFLATSLR AAAKERGPEV\par + H 121 131 141 151 161 171\par + V 181 191 201 211 221 231\par + ASTFKVLRNV TVVLWSAYPV VWLIGSEGAG IVPLNIETLL FMVLDVSAKV GFGLILLRSR\par + **** * *** *** * ** **** * * ***** ****** *** *** ******\par + ASTFNTLTAL VLVLWTAYPI LWIIGTEGAG VVGLGIETLL FMVLDVTAKV GFGFILLRSR\par + H 181 191 201 211 221 231\par + V 241 251 261\par + AIFGEAEAPE PSAGDGAAAT SD\par + ** * **** **** * *\par + AILGDTEAPE PSAG-AEASA AD\par + H 241 251 261\par +Conservation 56.1%\par +\pard \li220\ri240\sl220\keepn\brdrl\brsp100\brdrth \brdrb\brsp100\brdrth \brdrr\brsp100\brdrth Number of padding characters inserted 0 and 2\par +\pard\plain \s8\qj\fi-1140\li1140\sb120\sa120\sl240\tx1140 \f21\fs20 Figure 14.9\tab A run of SIPL using an entry from a sequence library and a file of entries to be excluded from the search.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 3.\tab Notes\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1.\tab +The variants on the proportional algorithm are selected by setting parameters using a special menu. This includes the facility to switch off the main diagonal for all options, which is useful when comparing a sequence against itself.\par +2.\tab For nucleotide sequences the program also has a function to complement a sequence. If the sequence on one axis is the complement of that on the other, the plots will show possible base pairing.\par +3.\tab When the cross hair is being employed, in addition to the standard special keys, the letter m will produce a display showing all the identical sequence characters around the cross hair position. The display is in the form of a matrix.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4.\tab +Users should not be misled by the "Quick scan" algorithm. Its function is to perform rapid comparisons. The plots it produces may look quite striking because they will contain almost no background, however such plots tell nothing about the significance +of the similarities displayed.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 5.\tab By using the "Reposition plots" function users can display several dot matrix plots on the screen at the same time. In this way plots from several pairs of sequence comparisons can be viewed together. +\par +6.\tab The library search program SIPL is of limited use for searching the nucleic acid libraries because it does not deal properly with sequences longer than 20,000 characters, but simply truncates them.\par +\pard\plain \s5\sb400\sa60\sl320\tx560 \b\f20\fs28 4.\tab References\par +\pard\plain \s7\qj\fi-560\li560\sa120\sl280\tx560 \f20 1. Staden, R. 1982. An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. {\i Nucl. Acids Res}. {\b 10(9)}\:2951-2961.\par +2. McLachlan, A.D. 1971. Test for comparing related amino acid sequences. {\i J. Mol. Biol.} {\b 61}\:409-424.\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 3. Schwartz, R.M. and Dayhoff, M.O. 1978. Matrices for detecting distant relationships. (in) {\i Atlas of Protein Sequence and Structure,} {\b 5 suppl. 3}\:353-358, Nat. Biomed. Res. Found., Washington D.C. +\par +\pard \s7\qj\fi-560\li560\sa120\sl280\tx560 4. Lipman, D.J. and Pearson, W.R. 1985. Rapid and sensitive protein similarity searches. {\i Science} {\b 227}\:1435-1441.\par +5.\tab Myers, E.W. and Miller, W. 1988. Optimal alignments in linear space. {\i Comput. Applic. Biosci}., {\b 4}, 11-17.\par +} diff --git a/doc/ted.PS b/doc/ted.PS new file mode 100644 index 0000000..88ef25a --- /dev/null +++ b/doc/ted.PS @@ -0,0 +1,3033 @@ +%! for use by dvi2ps Version 2.00 +% $Header: tex.ps,v 2.0 88/06/07 15:12:32 peterd Rel2 $ +% a start (Ha!) at a TeX mode for PostScript. +% The following defines procedures assumed and used by program "dvi2ps" +% and must be downloaded or sent as a header file for all TeX jobs. + +% By: Neal Holtz, Carleton University, Ottawa, Canada +% +% +% June, 1985 +% Last Modified: Aug 25/85 +% oystr 12-Feb-1986 +% Changed @dc macro to check for a badly formed bits in character +% definitions. Can get a <> bit map if a character is not actually +% in the font file. This is absolutely guaranteed to drive the +% printer nuts - it will appear that you can no longer define a +% new font, although the built-ins will still be there. +% mackay 4-Jan-1988 +% Changed size of character array to reflect gf usage (256 characters) + +% To convert this file into a downloaded file instead of a header +% file, uncomment all of the lines beginning with %-% + +%-%0000000 % Server loop exit password +%-%serverdict begin exitserver +%-% systemdict /statusdict known +%-% {statusdict begin 9 0 3 setsccinteractive /waittimeout 300 def end} +%-% if + +/TeXDict 200 dict def % define a working dictionary +TeXDict begin % start using it. + + % units are in "dots" (300/inch) +/Resolution 300 def +/Inch {Resolution mul} def % converts inches to internal units + +/Mtrx 6 array def + +%%%%%%%%%%%%%%%%%%%%% Page setup (user) options %%%%%%%%%%%%%%%%%%%%%%%% + +% dvi2ps will output coordinates in the TeX system ([0,0] 1" down and in +% from top left, with y +ive downward). The default PostScript system +% is [0,0] at bottom left, y +ive up. The Many Matrix Machinations in +% the following code are an attempt to reconcile that. The intent is to +% specify the scaling as 1 and have only translations in the matrix to +% properly position the text. Caution: the default device matrices are +% *not* the same in all PostScript devices; that should not matter in most +% of the code below (except for lanscape mode -- in that, rotations of +% -90 degrees resulted in the the rotation matrix [ e 1 ] +% [ 1 e ] +% where the "e"s were almost exactly but not quite unlike zeros. + +/@letter + { letter initmatrix + 72 Resolution div dup neg scale % set scaling to 1. + 310 -3005 translate % move origin to top (these are not exactly 1" + Mtrx currentmatrix pop % and -10" because margins aren't set exactly right) + } def + % note mode is like letter, except it uses less VM +/@note + { note initmatrix + 72 Resolution div dup neg scale % set scaling to 1. + 310 -3005 translate % move origin to top + Mtrx currentmatrix pop + } def + +/@landscape + { letter initmatrix + 72 Resolution div dup neg scale % set scaling to 1. +% -90 rotate % it would be nice to be able to do this + Mtrx currentmatrix 0 0.0 put % but instead we have to do things like this because what + Mtrx 1 -1.0 put % should be zero terms aren't (and text comes out wobbly) + Mtrx 2 1.0 put % Fie! This likely will not work on QMS printers + Mtrx 3 0.0 put % (nor on others where the device matrix is not like + Mtrx setmatrix % like it is on the LaserWriter). + 300 310 translate % move origin to top + Mtrx currentmatrix pop + } def + +/@legal + { legal initmatrix + 72 Resolution div dup neg scale % set scaling to 1. + 295 -3880 translate % move origin to top + Mtrx currentmatrix pop + } def + +/@manualfeed + { statusdict /manualfeed true put + statusdict /manualfeedtimeout 300 put % 5 minutes + } def + % n @copies - set number of copies +/@copies + { /#copies exch def + } def + +%%%%%%%%%%%%%%%%%%%% Procedure Defintions %%%%%%%%%%%%%%%%%%%%%%%%%% + +/@newfont % id @newfont - -- initialize a new font dictionary + { /newname exch def + pop + newname 7 dict def % allocate new font dictionary + newname load begin + /FontType 3 def + /FontMatrix [1 0 0 -1 0 0] def + /FontBBox [0 0 1 1] def +% mackay 4-Jan-1987 changed size of array from 128 to 256 for gf fonts + /BitMaps 256 array def + /BuildChar {CharBuilder} def + /Encoding 256 array def + 0 1 255 {Encoding exch /.undef put} for + end + newname newname load definefont pop + } def + + +% the following is the only character builder we need. it looks up the +% char data in the BitMaps array, and paints the character if possible. +% char data -- a bitmap descriptor -- is an array of length 6, of +% which the various slots are: + +/ch-image {ch-data 0 get} def % the hex string image +/ch-width {ch-data 1 get} def % the number of pixels across +/ch-height {ch-data 2 get} def % the number of pixels tall +/ch-xoff {ch-data 3 get} def % number of pixels below origin +/ch-yoff {ch-data 4 get} def % number of pixels to left of origin +/ch-tfmw {ch-data 5 get} def % spacing to next character + +/CharBuilder % fontdict ch Charbuilder - -- image one character + { /ch-code exch def % save the char code + /font-dict exch def % and the font dict. + /ch-data font-dict /BitMaps get ch-code get def % get the bitmap descriptor for char + ch-data null eq not + { ch-tfmw 0 ch-xoff neg ch-yoff neg ch-width ch-xoff sub ch-height ch-yoff sub + setcachedevice + ch-width ch-height true [1 0 0 1 ch-xoff ch-yoff] + {ch-image} imagemask + } + if + } def + + +/@sf % fontdict @sf - -- make that the current font + { setfont() pop + } def + + % in the following, the font-cacheing mechanism requires that + % a name unique in the particular font be generated + +/@dc % char-data ch @dc - -- define a new character bitmap in current font + { /ch-code exch def +% ++oystr 12-Feb-86++ + dup 0 get + length 2 lt + { pop [ <00> 1 1 0 0 8.00 ] } % replace <> with null + if +% --oystr 12-Feb-86-- + /ch-data exch def + currentfont /BitMaps get ch-code ch-data put + currentfont /Encoding get ch-code + dup ( ) cvs cvn % generate a unique name simply from the character code + put + } def + +/@bop0 % n @bop0 - -- begin the char def section of a new page + { + } def + +/@bop1 % n @bop1 - -- begin a brand new page + { pop + erasepage initgraphics + Mtrx setmatrix + /SaveImage save def() pop + } def + +%-- tjh sept. 87: if this page has a mac drawing on it, we have to +%-- use showpage in the md dictionary. +/@eop % - @eop - -- end a page + { + userdict /md known { + userdict /md get type /dicttype eq { + md /MacDrwgs known { + md begin showpage end + }{ + showpage + } ifelse + }{ + showpage + } ifelse + }{ + showpage + } ifelse + SaveImage restore() pop + } def + +/@start % - @start - -- start everything + { @letter % (there is not much to do) + } def + +/@end % - @end - -- done the whole shebang + { end + } def + +/p % x y p - -- move to position + { moveto + } def + +/r % x r - -- move right + { 0 rmoveto + } def + +/s % string s - -- show the string + { show + } def + +/c % ch c - -- show the character (code given) + { c-string exch 0 exch put + c-string show + } def + +/c-string ( ) def + +/ru % dx dy ru - -- set a rule (rectangle) + { /dy exch neg def % because dy is height up from bottom + /dx exch def + /x currentpoint /y exch def def % remember current point + newpath x y moveto + dx 0 rlineto + 0 dy rlineto + dx neg 0 rlineto + closepath fill + x y moveto + } def + +%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% +%% the \special command junk +%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% +% The structure of the PostScript produced by dvi2ps for \special is: +% @beginspecial +% - any number of @hsize, @hoffset, @hscale, etc., commands +% @setspecial +% - the users file of PostScript commands +% @endspecial + +% The @beginspecial command recognizes whether the Macintosh Laserprep +% has been loaded or not, and redfines some Mac commands if so. +% The @setspecial handles the users shifting, scaling, clipping commands + +%-- tjh sept. 87: made changes to allow postscript and macdrawing to +%-- to be inserted with version 65 of the md dictionary. Many bugs +%-- were fixed: +%-- vo changed to vof, name conflict with md +%-- vs changed to vsz, name conflict with md +%-- substantially changed @setspecial and @MacSetUp +%-- Also, made changes to allow users to specify offsets +%-- and clip rectangles in inches. + +% The following are user settable options from the \special command. + +/@SpecialDefaults + { /hs 8.5 72 mul def + /vsz 11 72 mul def + /ho 0 def + /vof 0 def + /hsc 1 def + /vsc 1 def + /CLIP false def + } def + +% d @hsize - specify a horizontal clipping dimension +% these 2 are executed before the MacDraw initializations +/@hsize {72 mul /hs exch def /CLIP true def} def +/@vsize {72 mul /vsz exch def /CLIP true def} def + +% d @hoffset - specify a shift for the drwgs +/@hoffset {72 mul /ho exch def} def +/@voffset {72 mul /vof exch def} def + +% s @hscale - set scale factor +/@hscale {/hsc exch def} def +/@vscale {/vsc exch def} def + +/@setclipper + { hsc vsc scale + CLIP + { newpath 0 0 moveto hs 0 rlineto 0 vsz rlineto hs neg 0 rlineto closepath clip } + if + } def + +% this will be invoked as the result of a \special command (for the +% inclusion of PostScript graphics). The basic idea is to change all +% scaling and graphics back to defaults, but to shift the origin +% to the current position on the page. Due to TeXnical difficulties, +% we only set the y-origin. The x-origin is set at the left edge of +% the page. + +/@beginspecial + { gsave /SpecialSave save def + % the following magic incantation establishes the current point as + % the users origin, and reverts back to default scalings, rotations + currentpoint transform initgraphics itransform translate + @SpecialDefaults % setup default offsets, scales, sizes + @MacSetUp % fix up Mac stuff + } def + + +%-- tjh: assume this is raw postscript, but save some state in case its not. +/@setspecial + { + /specmtrx matrix currentmatrix def + ho vof translate @setclipper + } def + + +/@endspecial + { SpecialSave restore + grestore + } def + + +% - @MacSetUp - turn-off/fix-up all the MacDraw stuff that might hurt us + % we depend on 'psu' being the first procedure executed + % by a Mac document. We redefine 'psu' to adjust page + % translations, and to do all other the fixups required. + % This stuff will not harm other included PS files +/@MacSetUp + { userdict /md known % if md is defined + { userdict /md get type /dicttype eq % and if it is a dictionary + { + md begin % then redefine some stuff + /psu % redfine psu to set origins, etc. + /psu load + % this procedure contains almost all the fixup code + { +% /letter {} def % it is bad manners to execute the real +% /note {} def % versions of these (clears page image, etc.) +% /legal {} def + /MacDrwgs true def + specmtrx setmatrix % restore pre-@setspecial state. + initclip % ditto + % change smalls to prevent page clearing. + /smalls [ lnop lnop lnop lnop lnop lnop lnop lnop lnop ] def + 0 0 0 0 ppr astore pop % prevents origin translation. + % redifine cp, do the showpage later, see @eop + /cp { + pop + pop + pm restore + } def % no printing of pages + } + concatprocs + def + /od + % redefine od to translate and scale. + % redfine load to set clipping region. + /od load + { + ho vof translate + hsc vsc scale + CLIP { + /nc + /nc load + { newpath 0 0 moveto hs 0 rlineto 0 vsz rlineto + hs neg 0 rlineto closepath clip } + concatprocs + def + } if + } + concatprocs + def + end } + if } + if + } def + +% p1 p2 concatprocs p - concatenate procedures +/concatprocs + { /p2 exch cvlit def + /p1 exch cvlit def + /p p1 length p2 length add array def + p 0 p1 putinterval + p p1 length p2 putinterval + p cvx + } def + +end % revert to previous dictionary +TeXDict begin @start +%%Title: ted.dvi +%%Creator: dvi2ps +%%EndProlog +5 @bop0 +[ 300 ] /cmr12.300 @newfont +cmr12.300 @sf +[ 24 33 -2 0 24.387] 50 @dc +[<70F8F8F870> 8 5 -4 0 13.548] 46 @dc +[ 32 34 -2 0 37.249] 68 @dc +[<00FC000703000E00801C0040380020780020700000F00000F00000F00000F00000F00000FFFFE0F000E07000E07801E03801 + C01C01C00C038007070001FC00> 24 21 -1 0 21.677] 101 @dc 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99 c +-1 r (ho) s +1 r (ose) s +16 r (to) s +16 r 99 c +-1 r (hange) s +15 r (the) s +16 r 118 c +0 r (ertical) s +15 r (magni\014cation) s +16 r (of) s +16 r (the) s +16 r (trace) s +16 r (data.) s +22 r 70 c +-3 r (ur-) s +164 1029 p (ther,) s +17 r (sequence) s +17 r (on) s +17 r (the) s +18 r (head) s +17 r (\(v) s +-1 r (ector\)) s +16 r (or) s +17 r (tail) s +18 r (\(uncertain) s +17 r (data\)) s +17 r (of) s +17 r (the) s +17 r (sequence) s +164 1090 p (ma) s +0 r 121 c +19 r 98 c +1 r 101 c +21 r (\\cuto\013) s +3 r 34 c +20 r (using) s +21 r (the) s +20 r (adjust) s +20 r (left) s +21 r (and) s +20 r (righ) s +0 r 116 c +19 r (cuto\013) s +20 r (buttons.) s +34 r (Bases) s +20 r (can) s +164 1150 p 98 c +1 r 101 c +17 r (inserted,) s +16 r (deleted,) s +17 r (or) s +16 r (replaced) s +17 r (as) s +16 r (with) s +17 r (an) s +0 r 121 c +15 r (ordinary) s +17 r 119 c +-1 r (ord-pro) s +1 r (cessor) s +16 r (in) s +17 r (the) s +164 1210 p (sequence) s +17 r (data) s +16 r 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+[<01F000071C000C06001C07003803803803807803C07001C07001C07001C0F001E0F001E0F001E0F001E0F001E0F001E0F001 + E0F001E0F001E0F001E0F001E0F001E0F001E0F001E07001C07001C07001C07001C03803803803801803000C0600071C0001 + F000> 24 34 -2 1 24.387] 48 @dc +[<01F000070C000C06001C03001803803801C03801C07001E07001E07001E0F001E0F001E0F001E0F001E0F001E0F001C0F801 + C0F80380F40300F40600F30C00F0F8007000007000007800003800003800001801801C03C00E03C00601C003008001C10000 + 7E00> 24 34 -2 1 24.387] 54 @dc +cmbx12.300 @sf +[ 40 34 -2 0 41.798] 82 @dc +cmr12.300 @sf +[<01FFF0001F00000E00000E00000E00000E00000E00000E00000E00FFFFF8800E00400E00200E00200E00100E00100E00080E + 00040E00040E00020E00020E00010E00008E00008E00004E00004E00002E00001E00001E00000E00000E00000600000200> 24 33 -1 0 24.387] 52 @dc +4 @bop1 +cmbx12.300 @sf +164 307 p (APPLICA) s +-4 r (TIONS) s +18 r (AND) s +19 r (CONCLUSIONS) s +cmr12.300 @sf +164 400 p (In) s +18 r (the) s +18 r (C.) s +19 r (elegans) s +18 r (genome) s +18 r (sequencing) s +19 r 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(program) s +15 r (has) s +15 r 98 c +1 r (een) s +15 r (in) s +0 r 118 c +-2 r (ok) s +-1 r (ed.) s +20 r (Other) s +15 r (parameters) s +15 r (whic) s +0 r 104 c +14 r (the) s +15 r (user) s +15 r (ma) s +-1 r 121 c +14 r (sp) s +2 r (ecify) s +15 r (on) s +164 367 p (the) s +15 r (command) s +15 r (line) s +15 r (include:) s +21 r (the) s +15 r (output) s +15 r (\014le) s +16 r (name;) s +15 r 97 c +15 r (base) s +15 r 112 c +2 r (osition) s +15 r (or) s +15 r (sequence) s +164 428 p (string) s +17 r (on) s +18 r (whic) s +0 r 104 c +16 r (the) s +17 r (trace) s +18 r (is) s +17 r (to) s +18 r 98 c +1 r 101 c +18 r (cen) s +-1 r (tered;) s +17 r 97 c +18 r (default) s +17 r (trace) s +18 r (magni\014cation;) s +18 r 97 c +164 488 p (5') s +17 r 118 c +-1 r (ector) s +16 r (sequence) s +16 r (for) s +17 r (automated) s +17 r (elimination) s +16 r (of) s +17 r (the) s +16 r (sequence) s +17 r (head) s +17 r (\(v) s +-1 r (ector\);) s +164 548 p (top) s +16 r (or) s +17 r 98 c +1 r 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It provides facilities +for viewing sequence and trace data (in top or bottom strand +orientation), for editing the base sequence, for +automated or manual trimming of the head (vector) and tail +(uncertain data) from the sequence, for vertical and horizontal trace +scaling, for keeping a history of sequence editing, and for output of +the edited sequence. Ted has been used extensively in the C. +elegans genome sequencing project, +both as a stand-alone program and integrated into +the Staden sequence assembly package, and has +greatly aided in the efficiency +and accuracy of sequence editing. It runs in the X +windows environment on Sun workstations and is available from the +authors. Ted currently supports sequence and trace data from the ABI +373A and Pharmacia A.L.F. sequencers. + +\subsubsection*{INTRODUCTION} + Time involved in sequence editing is extensive, and anything easing +that burden will improve the efficiency of any major sequencing +project. Having sequence and trace data available online in easily- +manipulable form is invaluable. Ted (a Trace-EDitor) was developed to +fill this role in the C. elegans genome +sequencing project [1]. + +\subsubsection*{METHODS} + +{\em Computing Design and Implementation.} +When designing ted, we had a number of specific computing goals +in mind including portability and adaptability. For portability, we +chose to write ted in ANSI C using the X windowing system and the +Xaw toolkit. X provides basic capabilities for the creation and use +of windows, and the toolkit contains a number of pre-packaged +components, such as the ``sliders'' used for scrolling. X also allows +site, user and per-run defaults to be set. Adaptability is also an +important goal since we are providing a new function to +research groups who are constantly adding new requirements. + + Stylistically, we have followed an ``Abstract Data Type'' +discipline. In this discipline, a program is split into a number of +modules which provide separate, well-defined functions. We +separate the interface of a module from its implementation. For +example, a unified internal sequence format is used. This can store +a varying amount of information. However, there is a clear and +simple interface by which the rest of the program accesses this +module. Such a style is not well supported by C, but its adoption has +been very successful. The addition of new sequencing machines, and +thus new external data formats, may cause some changes in the +internal representation of the sequence but should not affect +the rest of the program. + + Ted accepts a large number of optional command line arguments, +many of which can also be specified as system defaults. This +supports a mode of working whereby ted is invoked not directly by the +user but instead by a script or another application which supplies +arguments appropriate to the editing task. + + +{\em Graphical Interface.} +Ted currently accepts data from two fluorescence based sequencing +machines, the Pharmacia A.L.F. and the ABI 373A. +The sequencing machine data consists of +four traces of fluorescence levels together with the machine's +interpretation, which is a sequence of bases. +Ted displays +the traces and the machine-generated base list. +A second, initially identical, list of bases is provided for correction +by the user. + + Ted has an X windows based +graphical interface. The trace file +can either be input from the command line or by +clicking on the INPUT button after the program has been invoked. +Other parameters which the user may specify on the +command line include: the output +file name; a base position or sequence string on which the trace is +to be centered; a default trace magnification; a 5' vector sequence +for automated elimination of the sequence head (vector); top or +bottom strand orientation; or any of the usual X-window parameters (e.g. +display, geometry...). + + The graphics display (Figure 1) consists of the control +panel, the base position information, the original and edited sequence +data, and the graphical representation of the trace. The user may +begin by using the control panel INPUT button to input a new trace +file at which time the user selects whether to view the sequence +and trace in top or bottom strand orientation. +The trace file is displayed and, if a 5' vector sequence has been +specified on the command line, the program attempts to select a +cutoff point corresponding to the vector sequence at the ``head'' of the +trace file. The bases beyond the ``cutoff'' point are +displayed on a shaded background. The user may modify the cutoff +position by clicking on the ``Adj left cut'' button and clicking on the +position of the desired cutoff. Similarly, the user may adjust the +right cutoff of the sequence (chosen by starting at the 5' end of the +sequence and looking for the first occurrence when 2 out of 5 bases +are 'N') by scrolling along the sequence to that point, clicking on the +``Adj right cut'' button, and clicking on the appropriate base. +Automation of the ``cutoff'' process is optional; the user may compile +the program with that feature turned ``off.'' + + Clicking on the ``Edit seq'' button allows the user to enter the edit +mode. The ``Search'' button can be used to skip from ``problem'' to +``problem'' (i.e., ambiguity to ambiguity) or to look for runs of +identical bases (e.g., TTTT) which are often mis-called by +the machine software. + + Bases can be inserted, deleted, or replaced as with +any ordinary word-processor. In difficult-to-read areas, +the trace may be vertically or horizontally scaled by dragging or +clicking on the magnification scroll bar or by clicking on the +vertical scaling buttons (``Scale down'', ``Scale up''), respectively. +Finally, the edited sequence is saved to an ascii file using the +``Output'' button. A history of the editing session can also be saved +along with the sequence. +The ``Quit'' button is used +to exit the program. When reinvoking ted on an edited trace file the +edited base sequence, rather than the original sequence, is shown in +the edited base window. The user may invoke ted by calling in any one +of the previous editing sessions. + + +\subsubsection*{APPLICATIONS AND CONCLUSIONS} + + In the C. elegans genome sequencing project, data from the ABI or +A.L.F. sequencing machines' computers are transferred to Sun +workstations. +The user invokes a Unix shell script that calls ted systematically +on each of the new set of trace files creating a set of sequence files. +The sequence files that are deemed to be of acceptable quality +are then entered into the sequence +assembly program xdap [2] where the sequences are assembled into +contigs. Portions of the ted trace-editor have been incorporated +into the xdap ``trace manager,'' which is used in +conjunction with the contig editor to view sets of aligned traces +at sites of discrepancies in the aligned sequences. + + Ted is also used at the stage of choosing oligo primers for the +``walking'' stage of the sequencing project. It can be invoked directly +from the oligo selection program, osp [3], to allow examination +of the trace data in the region of the primers so that +integrity of the sequence data can be verified. + + Currently, no other programs are known to be available +which support editing of the ABI trace data. +Further, the modular design of the program should allow +support for new types of sequencing machines, with new data +formats, to be implemented in a straightforward fashion. + + +\subsubsection*{AVAILABILITY} + Ted is freely available from the authors or from Rodger Staden and +Simon Dear (MRC Laboratory of Molecular Biology, Hills Road, Cambridge, +UK, CB2 2QH) for use on Sun workstations running X-windows (or OpenLook). + + +\subsubsection*{ACKNOWLEDGMENTS} + The authors would like to thank all members of the C. elegans +sequencing project with special thanks to the following people: +John Sulston, Bob Waterston, +Phil Green, Rick Wilson, Richard Durbin, Simon Dear, and Rodger Staden +for their helpful suggestions for improvements in the ted interface +and for their parts in the development of ted. This work was +supported by the Medical Research Council and NIH grant R01-HG00136. + +\subsubsection*{REFERENCES} + +1. Waterston, R., Sulston, J., et al. (1991), in preparation. + +2. Dear, S. and Staden, R. (1991) Nuc. Acids Res., in press. + +3. Hillier, L. and Green, P. (1991) submitted. + + +{\bf Figure 1 legend.} + +Figure 1 shows a ``screen dump'' of the ted graphical interface. +The display consists of +the control panel and the synchronized view of the base position +information, original and edited sequence data, +and graphical representation of the trace (with each nucleotide's trace + being represented +by a different color). The control +panel allows the user to read in new trace files (in either +bottom or top strand orientation) +as well as to search for a string of nucleotides or a certain base position. +Scroll bars allow the user to adjust the magnification of or scroll through +the sequence and trace data. The user may also choose to change the vertical +magnification of the trace data. Further, sequence on the head (vector) +or tail (uncertain data) of the sequence may be ``cutoff'' +using the adjust left and right cutoff buttons. Bases can be inserted, +deleted, or replaced as with +any ordinary word-processor in the sequence data window. Finally, the +sequence may be written to an ascii file using the output button on +the control panel. + +\end{document} + + + diff --git a/help/BAP.RNO b/help/BAP.RNO new file mode 100644 index 0000000..731c8c9 --- /dev/null +++ b/help/BAP.RNO @@ -0,0 +1,2722 @@ +.npa +.left margin1 +@-1. TX 0 @General +.sp +@-2. T 0 @Screen control +.sp +@-2. X 0 @Screen +.sp +@-3. TX 0 @Modification +.sp +@0. TX -1 @BAP +.left margin2 +.PARA +This is an interactive program whose primary use is +for managing shotgun sequencing projects, but it can also be used for +handling alignments of other sequences, including those of proteins. +Currently the maximum 'gel reading' length is set to 4096 characters. +Almost all of the information below describes the use of the program for +shotgun projects, but those using the programs for handling other +sequence +alignments should interpret it accordingly. +The data for such a project is stored in a special type of database. The +program + contains the tools that are required to screen gel readings +against vector sequences and restriction sites, and to assemble +new gel +readings into the database (automatically comparing and aligning +them). In addition it contains editors and functions to examine the quality +of the aligned sequences. +.para + There are three main menus: "general", "screen" and "modification", +and some functions have submenus. +.left margin2 +.lit + The general menu contains the following options: + + Open a database + Display a contig + List a text file + Direct output + Calculate a consensus + Screen against restriction enzymes + Screen against vector + Check logical consistency + Copy database + Show relationships + set parameters + Highlight disagreements + Examine quality + Check Assembly + Find read pairs + +The graphics menu contains: + + Clear graphics + Clear text + Draw ruler + Use cross hair + Change margins + Label diagram + Plot map + Plot single contig + Plot all contigs + + +The modification menu contains: + + Edit contig + Auto assemble + Join contigs + Complement a contig + Alter relationships + Extract gel readings + Find internal joins + Disassemble readings + Shuffle pads + Auto-select oligos + Double strand + +The alter relationships menu contains: + + Cancel + Line change + Check logical consistency + Remove contig + Shift + Move gel reading + Rename gel reading + Break a contig + Remove a gel reading + Alter raw data parameters + +.END LIT +.SK1 +.para +Overview of the methodology +.para +The shotgun sequencing strategy +.para + In the shotgun sequencing procedure +the sequence to be determined is randomly broken into fragments of +about +1000 nucleotides in length. These fragments are cloned and then +selected randomly and their + + sequences determined. The relationship between any pair of + + fragments is not known beforehand +but is found by comparing their sequences. + + If the sequence of one found to be wholly or partially contained + + within that of another for sufficient length to distinguish an + + overlap from a repeat then those two fragments can be joined. +The + + process of select, sequence and compare is continued until the +whole + + of the DNA to be sequenced is in one continuous well +determined + + piece. + +.para + Definition of a contig + +.para + A CONTIG is a set of gel readings that are related to one + another by overlap of their sequences. All gel readings belong to + a contig and each contig contains at least one gel + reading. The gel readings in a contig can be summed to produce +a continuous consensus sequence and the length of this sequence is +the length of the contig. The rules used to perform this summation are + given under "the consensus algorithm". + At any stage + of a sequencing project the data will comprise a number of +contigs; +when a project is + + complete there should be only one contig and its consensus will be + the finished sequence. Note that since being introduced and +defined as above the word "contig" has been taken up by those involved in +genomic mapping. In that context the consensus with a precise length is, +of course, not +defined. + +.SK1 +.LEFT MARGIN2 +Introduction to the computer method +.LEFT margin2 +.PARA +It is useful to consider the objectives of a sequencing project before +outlining how we use the computer to help achieve them. The aim of a +shotgun sequencing project is to +produce an accurate consensus sequence from many overlapping gel +readings. +It is necessary to know, particularly at the latter +stages of the project, how accurate the +consensus sequence is. This enables us to know which regions of the + sequence require further work and also to know when the project is +finished. +To show the quality of the consensus, the programs described here +produce displays like that shown below. +.sk1 +.lit + + 10 20 30 40 50 + -6 HINW.010 GCGACGGTCTCGGCACAAAGCCGCTGCGGCGCACCTACCCTTCTCTTATA + CONSENSUS GCGACGGTCTCGGCACAAAGCCGCTGCGGCGCACCTACCCTTCTCTTATA + + 60 70 80 90 100 + -6 HINW.010 CACAAGCGAGCGAGTGGGGCACGGTGACGTGGTCACGCCGCGGACACGTC + -3 HINW.007 GGCACA*GTC + CONSENSUS CACAAGCGAGCGAGTGGGGCACGGTGACGTGGTCACGCCG-G-ACA-GTC + + 110 120 130 140 150 + -6 HINW.010 GATTAGGAGACGAACTGGGGCG3CGCC*GCTGCTGTGGCAGCGACCGTCG + -3 HINW.007 GATTAG4AGACGAACTGGGGCGACGCCCG*TGCTGTGGCAGCGACCGTCG + -5 HINW.009 GGCAGCGACCGTCG + 17 HINW.999 AGCGACCGTCG + CONSENSUS GATTAGGAGACGAACTGGGGCGACGCC-G-TGCTGTGGCAGCGACCGTCG + + 160 170 180 190 200 + -6 HINW.010 TCT*GAGCAGTGTGGGCGCTG*CCGGGCTCGGAGGGCATGAAGTAGAGC* + -3 HINW.007 TCT*GAGCAGTGTGGGCGCTGC*CGGGCTCGGAGGGCATGAAGTAGAGC* + -5 HINW.009 TCT*GAGCAGTGTGGGCG*T*G*CGGGCTCGGAGGGCATGAAGTAGAGC* + 17 HINW.999 TCTCGAGCAGTGTGGGCGCTG**CGGGCTCGGAGGGCATGAAGTAGAGCG + 12 HINW.017 GTAGAGC* + CONSENSUS TCT*GAGCAGTGTGGGCGCTG-*CGGGCTCGGAGGGCATGAAGTAGAGC* +.END LIT +.para + This is an example showing the left end of a contig from + position 1 to 200. Overlapping this region are gel readings +numbered 6, 3, 5, 17 and 12; +6, 3 and 5 +are in reverse orientation to their original reading (denoted by a minus +sign). Each gel reading also has a name (eg HINW.010). It can be seen that +in a number of places the sequences contain characters other than A,C,G +and +T. Some of these extra characters have been used by the sequencer to +indicate regions of uncertainty in the initial interpretation of the gel +reading, but the asterisks (*) have been inserted by the automatic +assembly function in order to align the sequences. Underneath each 50 +character block of gel reading sequences is the consensus derived from +the +sequences aligned above (the line labelled CONSENSUS). For most of its +length the consensus has a definite nucleotide assignment but in a few +positions there is insufficient agreement between the gel readings and +so a dash (-) appears in the sequence. This display contains all the +evidence needed to assess the quality of the consensus: the number of +times +the sequence has been determined on each strand of the DNA, and the +individual nucleotide assignments given for each gel reading. +.para +So the aim is to produce the consensus sequence and, equally important, +a display of the experimental results from which it was derived. +.para +In order to achieve this the following operations need to be performed: +.left margin2 +1) Put individual gel readings into the computer. +This might involved the manual interpretation of autoradiographs +or the transfer and process of machine-readable files from fluorescent +sequencing machines. +.left margin2 +2) Check each gel reading to make sure it is not simply part of one of the +vectors used to clone the sequence. +.left margin2 +3) Check each gel reading to make sure that those fragments that span +the +ligation point used prior to sonication are not assembled as single +sequences. +.left margin2 +4) Compare all the remaining gel readings with one another to assemble +them +to produce the consensus sequence. +.left margin2 +5) Check the quality of the consensus and edit the sequences. +.left margin2 +6) When all the consensus is sufficiently well determined, produce a copy +of +it for processing by other analysis programs. +.para +It is very unlikely that this procedure will only be passed through once. +Usually steps 1 to 5 are cycled through repeatedly, with step 4 just +adding +new sequences to those already assembled. Generally step 6 is also used +in +order to analyse imperfect sequence to check if it is the one the project +intended to sequence, or to look for interesting features. Analysis of +the consensus, such as +searches for protein coding regions, +can also help to find errors in the sequence. The display of the +overlapping gel readings shown above can be used to indicate, not only +the +poorly determined regions, but also which clones should be resequenced +to +resolve ambiguities, or those which can usefully be extended or +sequenced +in the reverse direction, to cover +difficult regions. + +.PARA +The original +individual gel readings for a sequencing project are each stored in +separate files. As the gel readings are entered into the computer +(usually in batches, say 10 +from a film), the file names they are given are stored in +a further file, called a file of file names. Files of file names +enable gel readings to be processed in batches. +.para +For each sequencing project +we start a project database. This database has a structure specifically +designed for +dealing with shotgun sequence data. +In order to arrive at the final consensus sequence many operations will +be +performed on the sequence data. Individual fragments must be +sequenced and +compared in both senses (i.e. both orientations) with all the other +sequences. When an overlap between a new gel reading and a contig are +found +they must be aligned and the new gel reading added to the contig. If a +new +gel reading overlaps two contigs they must be aligned and joined. Before +the two contigs are joined one of them may need to be turned around +(reversed and complemented) so they are both in in the same orientation. +.para +Clearly, keeping track of all these manipulations is quite complicated, +and to be able to perform the operations +quickly requires careful choice of data +structure and algorithms. For these reasons it is not practicable to store +the gel readings aligned as shown in the display above. Rather, it is more +convenient to store the sequences unassembled, and to record sufficient +information for programs to assemble them during processing. The +data used to assemble the sequences is called relational information. +.left margin2 +.PARA + The database comprises five files and they are described under the +section entitled "open database". +.PARA +Before entry into the project database +each new gel reading must be compared to look for overlaps +with all the data already contained +within the database. This last point is +important: all searching for overlaps is between individual new gel +readings and the data already in the database. There is no searching for +overlaps between sequences within the database; overlaps must be found +before new gel readings are entered into the database. +.para +Below I give an introduction to how the sequences are processed by +being +passed from one function to the next. +.para +This program is used to start a +database for the project and +then the following procedure is used. +.para +Data in the form of individual gel readings are entered into the computer + +and stored in separate files (possibly using either the digitizer + +program GIP). Batches +of these gel readings +are passed to the screening functions in this program to search for overlaps + +with vector sequences (see VEP and "screen against vector") or for matches to + +restriction enzyme sites that should not be + +present ("screen against enzymes"). +Each run of these screening functions passes on only those gel + +readings that do not contain unwanted sequences. Sequences are passed + +via +files of file names and eventually are processed by the automatic +assembly function ("auto assemble"). This function compares each gel +reading with a consensus of all the previous gel readings +stored in the database. +If it finds any +overlaps + it aligns the overlapping sequences by inserting padding characters, +and then adds the new gel reading to the database. +Gels that overlap are added to existing contigs and gels that do not +overlap any data in the database start +new contigs. If a new gel overlaps two contigs they are joined. +Any gel readings that appear to overlap but which +cannot be aligned sufficiently well are not entered and have +their names written to a file of failed gel reading names. +.PARA +Generally data is entered +into the database in batches as just described. The program + is also used to examine + +the data in the database, to enter gel readings that the automatic + +assembly function cannot align ("auto assemble"), + + and to make final edits. Edits to whole contigs + +can be made using a + mouse-driven editor ("edit contig"). + +.PARA +Editing the sequences is obviously an essential part of managing a + +sequencing project. +Editing is required when new + +sequences are added, when contigs are joined, and when sequences are + +corrected. +A basic part of the strategy + +used here is that new + +gel readings should be correctly aligned throughout their whole length + +when +they are entered into the database, and that when contigs are joined they + +are edited so that they are well aligned in the region of overlap. + + Alignment can be achieved by + +adding padding characters to the sequences, and this is the way "auto + +assemble" +operates when adding new sequences to the database. + +.para +In order to search +for overlaps that may have been missed or may be hidden in the "unused data" +the function "find internal joins" can be used. + +.para +Generally the users need not concern themselves with how the relational +information is used by the program, but it is necessary to know +how contigs are identified. Because contigs are constantly being changed and +reordered the program identifies them by the numbers of the gel readings +they contain. Whenever users need to identify a contig they need only +know +the number or name of one of the gel readings it contains. Whenever the +program asks users to identify a contig or gel reading they can type its +number or its archive name. If they type its archive name they must precede +the name by a slash "/" symbol to denote that it is a name rather than a +number. E.g if the archive +name is fred.gel with number 99, users should +type /fred.gel or 99 when asked to identify the contig. Generally, + when it asks for the gel reading to be identified, +the program will offer the user a default name, + and if the user types only return, that +contig will be accessed. When a database is opened the default contig will +be the longest one, but if another is accessed, it will subsequently become +the current default. +.para +Further information is located in the following places. +The database files are described under "open database". The format +for +vector and consensus sequences is given under "calculate a consensus", as are +the +uncertainty codes used in gel readings. +.left margin2 +.para + The digitiser program +is used for the initial input of gel readings +and for writing a file of file names. The program +uses a digitizer for data entry. +A digitizer is + a two dimensional surface such as a light box +which is such that if a special pen is pressed onto it, the pens +coordinates are recorded by a computer. +These coordinates + can be interpreted by a program. +.para + In order to read an autoradiograph placed on the light box +the user need only define the bottom of +the four sequencing lanes and the bases + to which they correspond and then use the pen to point to each + successive band progressing up the gel. The program examines +the + coordinates of each pen position to see in which of the four +lanes + it lies and assigns the corresponding base to be stored in the + computer. Each time the pen tip is depressed to point to a position + on the surface of the digitizer the program sounds the bell on the + terminal to indicate to the user that a point has been recorded. As + the sequence is read the program displays it on the screen. + +.left margin1 +@17. TX 1 @Screen against enzymes +.left margin2 +.PARA +Used to compare gel readings against any restriction enzyme recognition + +sequences that may have been used during cloning and which should not + +be present in the data. Works on single gel readings or processes batches + +accessed through files of file names. The algorithm looks for exact + +matches to recognition sequences stored in a file. + +.para +The file containing the recognition sequences must be identified. The +user +must choose between employing a file of file names, or typing in the + + +names of individual gel reading files. If a file of file names is used the + + +program will also create a new file of file names. When the option has + +finished operating this new file will contain the names of all those gel + +readings that did not match any of the recognition sequences. Hence it + can +be used for further processing of the batch. The recognition sequences + +should be stored in a simple text file with one recognition sequence per + +line. +.left margin1 +@18. TX 1 @Screen against vector +.left margin2 +.PARA +Used to compare gel readings against any vector sequences that may have + +been picked up during cloning and which have not been removed by vep. +It Works on single gel readings or processes + +batches accessed through files of file names. The algorithm looks for +exact +matches of length "minimum match length" and displays the overlapping + +sequences. +.para +The file containing the vector sequence must be identified. The user must + +choose between employing a file of file names, or typing in the names of + +individual gel reading files. If a file of file names is used the program +will +also create a new file of file names. When the option has finished + +operating this new file will contain the names of all those gel readings + +that did not match the vector sequence. Hence it can be used for further + +processing of the batch. The vector sequence should be stored in a simple + +text file with up to 80 characters of data per line. More than one vector + +can be stored in a single file. If so each should be preceded by a 20 + +character title of the form <---m13mp8.0001----> where the < and > + signs +and the number like .0001 are obligatory. The number must be preceded + +by a dot (.) and be 4 digits long. The total sequence in the file must be < + +500,001 characters in length. + +.left margin1 +@20. TX 3 @Auto assemble +.left margin2 +.PARA +Compares gel readings against the current contents of the database and + +produces alignments. In its normal mode of operation +("entry permitted"), the function +will automatically enter the gel readings into the database. +.para +New assembly suboption. +However +if entry is not permitted the reads won't be entered but the program +will produce alignments and (optionally) save each reading name and its best +alignment score (percentage mismatch) in a file. When used in +this mode, the program will include in the alignment the poor quality data +for each reading. These files of names can then be sorted into score order +and then used for assembly, hence forcing the readings that align best to +be entered into the database first. +End of new suboption. +.para +The routine works on + +single gel readings or processes batches of gel readings accessed through + +files of file names. It is the only way to enter data into the database. + +.para +The function will check the database for logical consistency and will + only +proceed if it is OK. Choose if gel readings should be entered into the + +database, or if they should only be compared. Choose between using a file + +of file names or typing file names on the keyboard. If so selected, supply + +the file of file names. Also supply a file of file names to contain the names of + +all the gel readings that fail to get entered. +Select the entry mode. Normal assembly is appropriate for all but special +cases, as is "permit joins". Uses for the other modes are not documented +here. +Define a minimum initial + +match length. +Define the maximum number + +of padding characters allowed to be used in each gel reading to help + +achieve alignment, and the same for the number allowed in the contig for + +each gel reading. Finally define the maximum percentage mismatch to +be allowed for any gel reading to be entered into the database. If + +for any gel reading, either of these last three values is exceeded the gel + +reading will not be entered into the database. + +.para +In operation the function takes a batch of gel readings (probably passed + + on as a file of file names from one of the screening routines) and +enters them into a + database for a sequencing project. It takes each gel reading + in turn, + compares it with the current consensus for the database, it then + produces an alignment for any regions of the consensus it + overlaps; if this alignment is sufficiently good it then edits + both the new gel reading and the sequences it overlaps and adds +the + new gel reading to the database. The program then updates the +consensus + accordingly and carries on to the next gel reading. +.para + All alignments are displayed and any gel readings +that do match but that + + cannot be aligned sufficiently well have their names written to a + file of failed gel reading names. The function works without any + + user intervention and can process any number of gel readings in a + single run. Those gel readings that fail can be recompared using + + the same function (to find the current overlap position) and the + +user can enter them into the database + + using the "put all readings in new contigs" +assembly option and then joined using "join contigs". +.para +Typical dialogue and output from the function is shown below. (Note that +output for gel readings 2 - 9 has been deleted to save space). +.lit +Automatic sequence assembler +Database is logically consistent +? (y/n) (y) Permit entry +? (y/n) (y) Use file of file names +? File of gel reading names=demo.nam +? File for names of failures=demo.fail +Select entry mode +X 1 Perform normal shotgun assembly + 2 Put all sequences in one contig + 3 Put all sequences in new contigs +? Selection (1-3) (1) = +? (y/n) (y) Permit joins +? Minimum initial match (12-4097) (15) = +? Maximum pads per gel (0-25) (8) = +? Maximum pads per gel in contig (0-25) (8) = +? Maximum percent mismatch after alignment (0.00-15.00) (8.00) = + >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> + Processing 1 in batch + Gel reading name=HINW.004 + Gel reading length= 283 + Searching for overlaps + Strand 1 + Strand 2 + No matches found + Total matches found 1 + Padding in contig= 0 and in gel= 1 + Percentage mismatch after alignment = 1.8 + Best alignment found + 1 11 21 31 41 51 + TTTTCCAGCG TGCGTCTGAC GCTGTCTTGC TTAATGATCT CCATCGTGTG CCTAGGTCTG + ********** ********** ********** ********** ********** ********** + TTTTCCAGCG TGCGTCTGAC GCTGTCTTGC TTAATGATCT CCATCGTGTG CCTAGGTCTG + 1 11 21 31 41 51 + 61 71 81 91 101 111 + TTGCGTTGGG CCGAGCCCAA CTTTCCCAAA AACGTATGGA TCTTACTGAC GTACA-GTTG + ********** ********** ********** ********** ********** ***** **** + TTGCGTTGGG CCGAGCCCAA CTTTCCCAAA AACGTATGGA TCTTACTGAC GTACACGTTG + 61 71 81 91 101 111 + 121 131 141 151 161 171 + CTTACCAGCG TGGCTGTCAC GGCGTCAGGC TTCCACTTTA GTCATCGTTC AGTCATTTAT + ********** ********** ********** ********** ********** ********** + CTTACCAGCG TGGCTGTCAC GGCGTCAGGC TTCCACTTTA GTCATCGTTC AGTCATTTAT + 121 131 141 151 161 171 + 181 191 201 211 221 231 + GCCATGGTGG CCACAGTGAC G-TATTTTGT TTCCTCACGC TCGCTACGTA TCTGTTTGCC + ********** ********** * ******** ********** ********** ********** + GCCATGGTGG CCACAGTGAC GCTATTTTGT TTCCTCACGC TCGCTACGTA TCTGTTTGCC + 181 191 201 211 221 231 + 241 251 261 271 281 + CGCG--GTGG AATTACAGCG TTCCCTATTG ACGGGCGCAT CCAC + **** **** ********** ** * ***** ********** **** + CGCGACGTGG AATTACAGCG TT,CDTATTG ACGGGCGCAT CCAC + 241 251 261 271 281 + Batch finished + 9 sequences processed + 0 sequences entered into database + 0 joins made + +.end lit + +.para +Note that "auto assemble" cannot align protein sequences. +.left margin1 +@28. TX 1 @Highlight disagreements +.left margin2 +.para +Used in the latter stages of a project +to highlight disagreements between individual gel readings +and their consensus sequences. This display is also availbale in the +contig editor. +Characters that agree with the + +consensus are shown as : symbols for the plus strand and . for the minus + +strand. Characters that disagree with the consensus are left unchanged + +and so stand out clearly. The results of this analysis are written to a +file. + +.para +Before selecting this option create a file of the display of the contig to +be +"highlighted". The option will ask for the name of this file. Select + symbols +to denote "agreeing" characters on each strand, the defaults are : and ., + +but any others can be used. Supply the name of a file in which to put + +the output. +.para +The display file needed as input for this option is created by selecting + +"Redirect output", followed immediately by "display contig", and then +"Redirect output" again. The + +cutoff score used in the consensus calculation can be set by option "set + +display parameters". Note that for the highlight function +there is a limit of 50 for the number of gel +readings that are aligned at any position - ie the contig must be less +than 51 gel readings deep at its thickest point. I hope that those performing +shotgun sequencing never reach this limit, but those using the program for +comparing sequence families might. +.para +Typical output from this function is shown below. +.lit + + 210 220 230 240 250 + 1 HINW.004 :C::::::::::::::::::::::::::::::::::::::::::AC:::: + 7 HINW.018 :*::::::::::::::::::::::::::::::::::::::::::CA:::: + -4 HINW.017 ...............AC.... + G-TATTTTGTTTCCTCACGCTCGCTACGTATCTGTTTGCCCGCG--GTGG + + 260 270 280 290 300 + 1 HINW.004 ::::::::::::*:D::::::::::::::::::: + 7 HINW.018 ::::::::::::::::::::CA:::::T:*:::*::::::::::::CA: + -4 HINW.017 ..............................................A... + 3 HINW.009 :::::::::::::::V::::::::::::::::::::::::::::*AV::: + -6 HINW.028 ......................A... + AATTACAGCGTTCCCTATTGACGGGCGCATCCACGCTGATTCTCTT-CTG + +.end lit +.left margin1 +@32. TX 3 @Extract gel readings +.left margin2 +.para +Used to make copies of the aligned gel readings in a database, +to write them into separate files, and to write a + +corresponding file of file names. It operates in two modes: either all gel + +readings are extracted, or only those at the ends of contigs. + +.para +Choose which mode of operation is required and supply a file of file + +names. +.para +The gel readings are given their original + +names. +.para +If the option is used to extract all the gel readings from a database, a + +subsequent run of "auto assemble" can reconstitute a database which has + +been corrupted. This rarely occurs and is usually necessitated by a + +user employing "alter relationships" incorrectly without first having + +made a copy. +.left margin1 +@1. TX 0 @Help +.left margin2 +.PARA +Help is available on the following topics : + +.LEFT MARGIN1 +@2. TX 0 @Quit +.LEFT MARGIN2 +.PARA +This command stops the program and is the only safe way to terminate a + +run +of the program that has altered the contents of the database in any way. + +.left margin1 +@3. TX 1 @Open a database +.LEFT MARGIN2 +.PARA +Opens existing databases or allows new ones to be started. The function + is +automatically called into operation +when the program is started but can also be selected + +from the general menu. +.para +Choose to open an existing database or start a new one, or if ! is typed +when the program is first started, enter the program without opening a +database. Supply a project + +database name, and if it already exists, the "version". If starting a new + +database define the database size and if it is for DNA or protein sequences. +The database size is an initial size for the database. It can be increased +later during the project. It is the sum of the number of gel +readings plus the number of contigs. The current maximum size is 8000. +.para +Database names can have from one to 12 letters and must not include full + +stop (.). The database is made from five separate files. If the database + is +called FRED then version 0 of database FRED comprises files FRED.AR0, + +FRED.RL0, FRED.SQ0, FRED.TG0 and FRED.CC0. The version is the last symbol in the file names. + +Only this program + can read these files. If the "copy database" option is used it + +will ask the user to define a new "version". +.para +For normal use the maximum gel reading length is set to 512 characters, + +but when a database is started the user may choose lengths of either + + 512, +1024, 1536..., 4096. Normally the program is used to handle DNA + +sequences but many of the functions also work on protein sequences. The + +choice of sequence type is made when the database is started. + +.para +The contigs are not stored on the disk as the user sees them displayed on + +the screen. Each gel reading is stored with sufficient information about + +how it overlaps other gel readings so that the program can work out how + +to +present them aligned on the screen. We refer to this extra data as "the +relationships" and it is explained below. + +The database comprises 5 separate files. + +.left margin2 + 1. a working version of each gel reading. This is the version of + the gel reading +that is in the database and initially it is an exact copy of + the original sequence (known as the archive) + but it is edited and manipulated to align it + with other gel readings. + +.left margin2 + 2. the file of relationships. This file contains all of the + + information that is required to assemble the working versions +into + + contigs during processing; any manipulations on the data use this + + file and it is automatically updated at any time that the + + relationships are changed. The information in this file is as + + follows: +.left margin2 + (A) Facts about each gel reading and its relationship to +others +("gel + + descriptor lines"): + +.left margin2 + (a) the number of the gel +reading (each gel reading is given a number as it is + + entered into the database) + +.left margin2 + (b) the length of the sequence from this gel reading + +.left margin2 + (c) the position of the left end of this gel +reading relative to the left + + end of the contig of which it is a member + +.left margin2 + (d) the number of the next gel +reading to the left of this gel reading + +.left margin2 + (e) the number of the next gel reading to the right + +.left margin2 + (f) the relative strandedness of this gel +reading , ie whether it is in + + the same sense or the complementary sense as its archive. + +.left margin2 + (B) Facts about each contig ("contig descriptor lines"): + +.left margin2 + (a) the length of this contig + +.left margin2 + (b) the number of the leftmost gel +reading of this contig + +.left margin2 + (c) the number of the rightmost gel reading of this contig. + +.left margin2 + (C) General facts: + +.left margin2 + (a) the number of gel readings in the database + +.left margin2 + (b) the number of contigs in the database. + +.left margin2 + 3. the file of archive names. This is simply a list of the names + + of each of the archive files in the database. + +.left margin2 + 4. the file of tags (annotation). +This consists of linked lists of tag information for each sequences in the +database. +Tags are created by the user as annotation, or by xdap as records of edits or +for storing cutoff information. +As the number of tags can grow without limit, so can this file. +For each gel there is a header record, which contains the record number of +the start of the linked list for that gel. On line IDBSIZ there is a record +containing information about the file such as its present length and if there +are any free "tag" slots to be reused in the file. + + 5. the file of comments (annotation). +This consists of linked lists of comment fragments. +Comments are created by the user as a message attached to annotation, +or by the system to store cutoff information. +Comments are character strings of any length. +Comments longer than 40 characters are broken up into fragments, each 40 +characters long, and are chained together in a link list. +As the number of comments can grow without limit, so can this file. + +.para + Structure of the database files +.para + 1. The file of relationships +.para + The file contains IDBSIZ lines of data: + the general data are stored on line IDBSIZ; data about gel +readings are + stored from line 1 downwards; data about contigs are stored from + line IDBSIZ-1 upwards. A database of 500 lines containing 25 gel +readings and 4 contigs would have a file + of relationships as is shown below. +.lit + + + --------------------------------------------- + 0 Info about the database size + 1 Gel descriptor record + 2 " " " + 3 " " " + 4 " " " + 5 " " " + ' ' ' ' + ' ' ' ' + 25 " " " + 26 Empty record + ' ' ' + + ' ' ' + 495 ' ' + 496 Contig descriptor record + 497 " " " + 498 " " " + 499 " " " + 500 Number of gel readings=25, Number of contigs=4 + --------------------------------------------- + + The arrangement of the data in the file of relationships + +.end lit +As each new gel reading is added into the database a new line is added + to the end of the list of gel descriptor + lines. If this new gel reading does not + overlap with any gel readings + already in the database a new contig line is + added to the top of the list of contig lines. If it overlaps with + one contig then no new contig line need be added but if it overlaps + with two contigs then these two contigs must be joined and the + number of contig lines will be reduced by one. Then the list of +contig + lines is compressed to leave the empty line at the top of the list. + Initially the two types of line will move towards one another but + eventually, as contigs are joined, the contig descriptor lines will + move in the same direction as the gel descriptor + lines. At the end of a + project there should be only one contig line. The database is thus + capable of handling a project of 998 gels. +.para + 2. Structure of the working versions file +.para + The working versions of gel readings are stored in a file of + NGELS lines each containing MAXGEL characters. Gel reading +number 1 is stored on line + 1, gel reading number 2 on line 2 and so on. NGELS is the +current number of readings and MAXGEL the maximum reading length. +.para + 3. Structure of the archive names file +.para + This file has NGELS lines of 16 characters. + +.para + 4. Structure of the tag file +.para +This file initially starts with IDBSIZ lines, and is expanded as new tags are +created. +Information about the length of the file, and which tag records are reusable +is stored on line IDBSIZ. +A database of 500 lines would have a file of tags as shown below. +.lit + + --------------------------------------------- + 1 Tag descriptor record + 2 " " " + 3 " " " + 4 " " " + 5 " " " + ' ' ' ' + ' ' ' ' + 497 " " " + 498 " " " + 499 " " " + 500 Length of file=N, Free list=0 + 501 Tag record + 502 " " + 503 " " + ' ' ' + ' ' ' + N-2 " " + N-1 " " + N Tag record + --------------------------------------------- + + The arrangement of the data in the tag file + +.end lit +As each new tag is added to the database, a check is made in the +file descriptor record at line IDBSIZ. If the list of reusable records is 0, +the file is extended by one line. Otherwise the new tag is assigned to +record at the head of the freelist. +When tags are deleted, they are added to the free list in the file descriptor +record. +.para + 5. Structure of the comment file +.para +This file initially starts with 1 line, and is expanded as new annotation is +created. +Information about the length of the file, and which comment records are reusable +is stored on the first line. +.lit + + --------------------------------------------- + 1 Length of file=N, Free list=0 + 2 Comment fragment + 3 " " + 4 " " + ' ' ' + ' ' ' + N-2 " " + N-1 " " + N Comment fragment + --------------------------------------------- + + The arrangement of the data in the comment file + +.end lit +As each new comment is added to the database, a check is made in the file +descriptor record at line 1. If the list of reusable records is 0, +the file is extended to hold the new comment. Otherwise the new comments is +assigned to records starting with the head of the freelist. +When comments are deleted, the discarded records are added to the free list in +the file descriptor record. +.para + There are various checks within the programs to + protect users from themselves:- +.left margin2 + 1. All user input is checked for errors - e.g. reference to + non-existent gel +readings or contigs, incorrect positions in the + contig or gel readings. +.left margin2 + 2. Before entering a gel reading the system checks to see if a + file of the same name has already been entered. +.left margin2 + 3. Join will not allow the circularising of a contig. + +.left margin2 +5. Users may escape from any point in the program. +.left margin2 +6. Help is available from all points in the program. +.SK2 +.LEFT MARGIN2 +IT IS ESSENTIAL THAT USERS DO NOT KILL THE PROGRAM WHILE IT IS +DOING +ANYTHING THAT INVOLVES CHANGING THE CONTENTS OF THE +DATABASE. I.E DURING AUTO ASSEMBLE, +COMPLETE JOIN, COMPLEMENT CONTIG, SAVE EDIT CONTIG. + +This could +corrupt the database so badly that it is impossible to fix. The program +should always be left using the QUIT option. + +.left margin1 +@4. TX 3 @Edit contig +.LEFT MARGIN2 +.PARA +The Contig Editor is a mouse-driven editor that can insert, +delete and change gel reading sequences. +.para +The Contig Editor allows scrolling from one end of a contig to the other +using the scroll bar and scroll buttons. Action of mouse button presses +when the mouse pointer is in the scroll bar: +.sk1 +.lit + Middle Mouse Button Set editor position + Left Mouse Button Scroll forward one screenful + Right Mouse Button Scroll backwards one screenful +.end lit +.sk1 +The four scroll buttons operate as follows: +.sk1 +.lit + "<<" Scroll left half a screenful + "<" Scroll left one character + ">" Scroll right one character + ">>" Scroll right half a screenful +.end lit +.para +The Editor cursor can be positioned anywhere in the edit window by +moving the mouse pointer over the character of interest, then pressing the +left mouse button. The Editor cursor can also be moved by using the +direction arrow keys. +.para +The editor operates in two main edit modes - Replace and Insert. Replace allows +a character to be replaced by another. Insert allows characters to be +inserted into a gel reading sequence. Characters are entered by typing +them from the keyboard. Only valid characters are permitted. +Characters can be deleted by positioning the cursor one character to the right, +then pressing the delete key. +Normally Insert and Delete apply to the consensus line of the contig ONLY. +This restraint can be overridden by using the "Super Edit" mode of +operation, THOUGH IT IS NOT RECOMMENDED. +.para +Edits can also be performed on the consensus, though they are +restricted to insertion and deletion of padding characters ("*"). +These edits also have special meanings. +A deletion will delete ALL characters at the position to the left +of the cursor in the contig, and move the relative positions of all +sequences starting to the right of the cursor position left one +character. +An insertion will insert the character typed ("*") into ALL gel +reading sequences at the cursors position in the contig, and move the +relative positions of all sequences starting to the right of the cursor +position right one character. +.para +The effect of the last edit can be undone by pressing the "Undo" button +at the top of the editor window. +.para +The cursor will automatically be positioned at the next problem when the +"Find Next Problem" button is selected. The next problem is where the +consensus shows either an ambiguity ("-") or a pad ("*") character. +.para +The edits to the contig can be saved by pressing the "Leave Editor" +button and replying "Yes" to the prompt to "Save changes?". As no changes +are made to the working copy of your database til this point it +is possible to abort the editor if +the edit session ends up in an unsatisfactory state (ie if you've +stuffed it up!) +.left margin1 +.sk3 +Displaying Traces +.left margin2 +.para +The original data from which the gel reading sequences where derived can +be seen by double clicking (two quick clicks) with the middle mouse button +on the area of interest. The trace will be displayed with the point +clicked at the centre of the trace viewport. +.para +All traces that are displayed are maintained in one window, called the Trace +Manager. The Trace Manager will only display four traces maximum. When four +traces are already being managed and a new one is requested, the one at the top +of the Trace Manager is removed and the new one is added to the bottom. +Traces can be removed individually by using the "quit" button in the panel next +to the trace. +.left margin1 +.sk3 +Extending Reads Using Cutoff Information +.left margin2 +.para +Sequence data read in from Automated Fluorescent sequencing machines +trace files processed through the program ted +will have the discarded sequence (vector at start and poor read at +end) available to the contig editor. To display the cutoff +information, press the "Display Cutoff" button at the top of the +editor window. +The cutoff sequence appears in grey. This sequence can be incorporated +into the editable sequence, by moving the cutoff position. This is +done by positioning the cursor at the end of the gel sequence, and +using Meta-Left-Arrow and Meta-Right-Arrow to adjust the point of cutoff. +The Meta key is a diamond on the Sun keyboard. +.left margin1 +.sk3 +Pop-up menu +.left margin2 +.para +A pop-up menu is revealed by depressing the "Control" key on the keyboard +and at the same time pressing the left mouse button. The menu has the following +functions: +.lit + + Search + Highlight Disagreements + Save Contig + Create Tag + Edit Tag + Delete Tag + Select Oligo + +.end lit +.left margin2 +"Highlight Disaggreements" simply toggles between the normal display showing +the current base assignments and one in which only those assignments that +differ from the consensus are shown. + +.left margin2 +"Save Contig" is described above. +Searching and operations on tags are described below. +.left margin2 +.sk3 +Searching +.left margin2 +.para +Selecting "Search" brings up a +window which can remain present during normal editor operation. The +window allows the user to select the direction of search, the type of +search and a value to search on. The value is entered into the value +text window. Then pressing the "search" button +performs the search. If successful, the cursor is positioned and +centred accordingly. An audible tone indicates failure. Pressing the +"ok" button removes the search window. The search window is +automatically removed when the contig editor is exited. +.sk1 +There are seven different search modes: +.sk1 +1. Search by position +.sk1 +This positions the cursor at the numeric position specified in the +value text window. Eg a value of "1234" causes the cursor to be placed +at base number 1234 in the contig. Positioning withing a gel reading is +achieved by prefixing the number with the "@" character, eg "@123" +positions the cursor at base 123 of the sequence in which the cursor +lies. Relative positions can be specified by prefixing the number with +a plus or minus character. Eg "+1234" will advance the cursor 1234 +bases. If possible, the cursor is positioned within the same sequence. +The direction buttons have no effect on the operation of "search +by position". +.sk1 +2. Search by reading name +.sk1 +This positions the cursor at the left end of the gel reading specified +in the value text window. If the value is prefixed with a slash is is +assumed to be a gel reading name. Otherwise it is assumed to be a gel +reading number. Eg "123" positions the cursor at the left end of gel +reading number 123. "/a16a12.s1" positions at the start of reading +a16a12.s1. If the value was "/a16" the cursor is positioned at the +first reading which starts with "a16". The direction buttons have no +effect on the operation of "search by position". +.sk1 +3. Search by tag type. +.sk1 +This positions the cursor at the start of the next tag which has the +the same type as specified by the type value menu. To change the type, +select off the menu that pops up when the mouse is clicked on the +button labeled "Type:". The search can be performed either forwards +or backwards of the current cursor position. To find all tags, use +"search by annotation", with a null text value string. +.sk1 +4. Search by annotation. +.sk1 +This positions the cursor at the start of the next tag which has a +comment containing the string specified in the value text window. The +search performed is a regular expression search, and certain +characters have special meaning. Be careful when your value string +contains ".", "*", "[", "^" or "$". The search can be performed either +forwards or backwards from the current cursor position. +.sk1 +5. Search by sequence. +.sk1 +This positions the cursor at the start of the next piece of sequence +that matches the value specified in the text value window. The search +is for an exact match, which means the case of value string is +important. The search is performed on the gel readings themselves, +rather than the consensus sequence. The search can be performed either +forwards or backwards from the current cursor position. +.sk1 +6. Search by problem. +.sk1 +This positions the cursor at the next place in the consensus sequence +which is not an "A", "C", "G" or "T". The search can be performed +either forwards or backwards from the current cursor position. +.sk1 +7. Search by quality +.sk1 +This positions the cursor at the next place in the consensus sequence +where the consensus calculation for each strand disagrees. When only +sequences on one strand is present, the search will stop at every +base. The search can be performed either forwards or backwards from the +current cursor position. +.left margin1 +.sk3 +Annotation +.left margin2 +.para +Parts of a sequence can be annotated, to record the positions of primers used +for walking, or to mark sites, such as compressions that have caused problems +during sequencing. +The consensus sequence CANNOT be annotated. +.para +To annotate a piece of sequence first select the part of sequence +using the mouse buttons. Use the left mouse button to position the start of the +selection, and while this button is being held down, move the mouse to extend. +The selection can be extended further using the right mouse button. +.para +To create annotation, invoke the pop-up menu, and select the "Create Tag" +function. A small "tag editor" will appear which +allows you to select the type of the +annotation from a pull-down menu, and specify a comment if desired. +To select a new type pull down the Type menu, and select the entry desired. +To enter a comment, simply type into the text window in the tag editor. +The annotation is created when the "Leave" button on the tag editor, +and is displayed in the colour defined in the tag database file (TAGDB). +.para +To edit existing annotation, +position the cursor with the left mouse button +on the tag, and select the +"Edit Tag" +off the pop-up menu. +This invokes the tag editor, and changes to the type and comment of the +annotation can be made. The tag is updated when the "Leave" button is pressed. +.para +To delete an existing annotation, +position the cursor with the left mouse button +on the tag, and select the +"Delete Tag" +off the pop-up menu. +.left margin1 +.sk3 +NOTE: +.left margin2 +.para +As the Contig Editor is a very powerful tool, it is possible that the alignment +of the gel reading sequences has unexpectedly been disrupted. +This can easily happen to parts of the contig that lie to the right +of the screen if excessive use has been made of the "Super Edit" facility. +Until familiar with "Super Edit" it would benefit the sequencer to quickly +scan through the contig after editing to check that bad alignments have not +been created. +.sp +.left margin2 +Selecting Oligos +---------------- +.sk1 +.left margin2 +1. Open the oligo selection window, by selecting "Select Oligo" from +the contig editor popup menu. + +.left margin2 +2. Position the cursor to where you want the oligo to be chosen. While +the oligo selection window is visible, you will still have complete +control over positioning and editing within the contig editor. + +.left margin2 +3. Indicate the strand for which you require an oligo. This is done by +toggling the direction arrow ("----->" or "<------"), if necessary. + +.left margin2 +3. Press the "Find Oligos" button to find all suitable oligos (See +"Oligo selection" below.) Information for the closest oligo to the +cursor position is given in the output text window. In the contig +editor the position of the oligo is marked by a temporary tag on the +consensus. The window is recentered if the oligo is off the screen. +Selecting "Display Selection Information" will print a short report on +the numbers of oligos considered and rejected during oligo selection. + +.left margin2 +4. If this oligo is not suitable (it may have been previously chosen, +and found to be unsuitable by experimentation, say), the next closest +oligo can be viewed by pressing "Select Next". + +.left margin2 +5. Suitable templates are automatically identified for the currently +displayed oligo (See "Template selection" below.) By default, the +template is that closest to the oligo site. If the choice is not +suitable (it may be known to be a poor quality template, say) another +can be chosen from the "Choose Template for this Oligo" menu. +Templates that do not appear on the menu can be specified by selecting +"other". However, the template must be on the correct strand and be +upstream of the oligo. + +.left margin2 +6. A tag can be created for the current oligo by pressing the button +"Create a tag for this oligo". The annotation for this tag holds the +name of the template and the oligo primer sequence. There are fields +to allow the user to specify their own primer name ("serial#") and +comments ("flags") for this tag. An example of oligo tag annotation: +.lit + serial#= + template=a16a9.s1 + sequence=CGTTATGACCTATATTTTGTATG + flags= + +.end lit +.left margin2 +7. The oligo selection window is closed when "Create a tag for this +oligo" or "Quit" is selected. + + +.left margin2 +Oligo selection: +.left margin2 +---------------- + +.left margin2 +The oligo selection engine is the one used in the program OSP. It is +described in some detail in: + +.left margin2 + Hillier, L., and Green, P. (1991). "OSP: an oligonucleotide + selection program," PCR Methods and Applications, 1:124-128. + +.left margin2 +The parameters controlling the selection of oligos can be changed in +the "Oligo Selection Parameters" window. The weights controlling the +scoring of selected oligos can be changed in the "Oligo Selection +Weights" window. + +.left margin2 +By default, the oligos are selected from a window that extends 40 +bases either side of the cursor. The size and location of this window +relative to the cursor position can be changed in the "Parameters" +window. + +.left margin2 +In xbap oligos are ranked according to their proximity to the cursor +position, rather than by their scores. + + +.left margin2 +Template selection: +.left margin2 +------------------- + +.left margin2 +For simplicity, each reading is considered to represent a template. In +practise, many readings can be made of the same template. Suitable +templates that are identified are those that: +.lit + + 1. are in the appropriate sense, + 2. have 5' ends that start upstream of the oligo, +and 3. are sufficiently close to the oligo to be useful. + +.end lit +.left margin2 + +This last criterion relates to the insert size for the subclones used +for sequencing and the average reading length. A template is +considered useful if a full reading can be made from it, taking into +account both of these factors. The default insert size is 1000 bases, +and the default average reading length is 400 bases. These values can +be changed in the "Parameters" window. + +.left margin1 +@5. TX 1 @Display a contig +.LEFT MARGIN2 +.para +Used to show the aligned gel readings for any part of a contig. The + +number, name and strandedness of each gel reading is shown and the + +consensus is written below. +.para +If required identify the contig, and then the start and end points of the + +region to display. +.para +The display can be directed to a disk file using "direct output to disk". + +.para + Below is an example showing the left end of a contig from + position 1 to 200. Overlapping this region are gels 6,3,5,17and 12; +6, 3 and 5 +are in reverse orientation to their archives (denoted by a minus sign) + There are a few uncertainty codes and a few padding + characters in the working versions, but the consensus (shown +below + each page width) has a definite assignment for almost every +position. +.lit + + 10 20 30 40 50 + -6 HINW.010 GCGACGGTCTCGGCACAAAGCCGCTGCGGCGCACCTACCCTTCTCTTATA + CONSENSUS GCGACGGTCTCGGCACAAAGCCGCTGCGGCGCACCTACCCTTCTCTTATA + + 60 70 80 90 100 + -6 HINW.010 CACAAGCGAGCGAGTGGGGCACGGTGACGTGGTCACGCCGCGGACACGTC + -3 HINW.007 GGCACA*GTC + CONSENSUS CACAAGCGAGCGAGTGGGGCACGGTGACGTGGTCACGCCG-G-ACA-GTC + + 110 120 130 140 150 + -6 HINW.010 GATTAGGAGACGAACTGGGGCG3CGCC*GCTGCTGTGGCAGCGACCGTCG + -3 HINW.007 GATTAG4AGACGAACTGGGGCGACGCCCG*TGCTGTGGCAGCGACCGTCG + -5 HINW.009 GGCAGCGACCGTCG + 17 HINW.999 AGCGACCGTCG + CONSENSUS GATTAGGAGACGAACTGGGGCGACGCC-G-TGCTGTGGCAGCGACCGTCG + + 160 170 180 190 200 + -6 HINW.010 TCT*GAGCAGTGTGGGCGCTG*CCGGGCTCGGAGGGCATGAAGTAGAGC* + -3 HINW.007 TCT*GAGCAGTGTGGGCGCTGC*CGGGCTCGGAGGGCATGAAGTAGAGC* + -5 HINW.009 TCT*GAGCAGTGTGGGCG*T*G*CGGGCTCGGAGGGCATGAAGTAGAGC* + 17 HINW.999 TCTCGAGCAGTGTGGGCGCTG**CGGGCTCGGAGGGCATGAAGTAGAGCG + 12 HINW.017 GTAGAGC* + CONSENSUS TCT*GAGCAGTGTGGGCGCTG-*CGGGCTCGGAGGGCATGAAGTAGAGC* +.END LIT +.left margin1 +@6. TX 1 @List a text file +.LEFT MARGIN2 +.PARA +This option allows users to list text files on the screen. It can be used +to read a file containing notes, for checking files written to disk etc. The +user is asked to type the name of the file to list. +.left margin1 +@8. TX 1 @Calculate a consensus +.LEFT MARGIN2 +.para + Calculates a consensus sequence either for the whole database or + +for selected contigs. The consensus is written to a file named by the + user. +.left margin2 +Supply a file name, choose between whole database or selected contigs. +.para + Symbols for uncertainty in gel readings +.para +In order to record uncertainties when reading gels the codes shown + +below can be used. Use of these codes permits us to extract the + +maximum amount of data from each gel and yet record any doubts by + +choice of code. The program can deal with all of these codes and any + +other characters in a sequence are treated as dash (-) characters. + + +.lit + + SYMBOL MEANING + + 1 PROBABLY C + 2 " T + 3 " A + 4 " G + D " C POSSIBLY CC + V " T " TT + B " A " AA + H " G " GG + K " C " C- + L " T " T- + M " A " A- + N " G " G- + R A OR G + Y C OR T + 5 A OR C + 6 G OR T + 7 A OR T + 8 G OR C + - A OR G OR C OR T + a A + c C + g G + t T + * padding character placed by auto assembler + else = - + +.end lit + +.LEFT MARGIN2 + The DNA consensus algorithm +.para +The "calculate consensus" function, the "display contig" routine and the + +"show quality" option use the rules outlined here to calculate a + +consensus from aligned gel readings. Note that "display contig" +calculates +a consensus for each page width it displays (it does not use the + +consensus sequence file calculated by the consensus function). + +.LEFT MARGIN2 +.para +We have 6 possible symbols in the consensus sequence: A,C,G,T,* and -. The +last symbols is assigned if none of the others makes up a sufficient +proportion of the aligned characters at any position in the contig. The +following calculation is used to decide which symbol to place in the +consensus at each position. +.para +Each uncertainty code contributes a score +to one of A,C,G,T,* and also to the total at each point. Symbols like R +and Y which don't correspond to a single base type contribute only to the +total at each point. The scores are shown below. +.lit + definite assignments ie A,C,G,T,B,D,H,V,K,L,M,N,a,c,g,t,* =1 + + probable assignments ie 1,2,3,4 = 0.75 + + other uncertainty codes including R,Y,5,6,7,8,- = 0.1 +.end lit +.para +A cutoff score of 51% to 100% is supplied by the user. (When the program +starts this is set to 75%. See "set display parameters"). +At each position in the contig we calculate the total score for each of +the 5 symbols +A,C,G,T and * (denote these by Xi, where i=A,C,G,T or *), +and also the sum of these totals +(denote this by S). Then if 100 Xi / S > the cutoff for any i, symbol i is +placed in the consensus; otherwise - is assigned. +.para +Notice that S does not equal the number of times the sequence has been +determined, but is the score total, and hence we are less likely to put a - +in the consensus. For the "examine quality" algorithm each strand is +treated separately but the calculation is the same. (It was originally +different). +.para +Format of the consensus sequence ( and vector sequences). +.para +A consensus sequence file may contain the consensus for several contigs + +and so we identify each of them by preceding them by a 20 character + +title. The title is of the form <---LAMBDA.0076----> ( where LAMBDA is + +the project name and gel reading number + + + 76 is the leftmost gel +reading to contribute to this consensus sequence). + + + The angle brackets <> and the 4 digit number precede by a . + +are important to some processing programs. +.left margin1 +@25. TX 1 @Show relationships +.LEFT MARGIN2 +.para + Used to show the relationships of the gel readings in the database in + +three ways - +.LEFT MARGIN2 + (a) All contig descriptor lines followed by all gel descriptor + lines. +.LEFT MARGIN2 + (b) All contigs one after the other sorted, i.e. for each + contig show its contig descriptor line followed by all its + gel descriptor lines sorted on position from left to right +.LEFT MARGIN2 + (c) Selected contigs: show the contig line and, in order, + those gel readings that cover a user-defined region. + Note that this output can be directed to a disk file by + prior selection of "redirect output". +.LEFT MARGIN2 +.para + Below is an example showing a contig from position + 1 to 689. The left gel reading is number 6 and has archive +name HINW.010, the +rightmost gel reading is number 2 and is has archive name HINW.004. +On each gel descriptor line is shown: + the name of the archive version, the gel number, the position of the + left end of the gel reading relative to the left end of the contig, the + length of the gel +reading (if this is negative it means that the gel reading is in + the opposite orientation to its archive), the number of the gel +reading to + the left and the number of the gel reading to the right. +.lit + + + CONTIG LINES + CONTIG LINE LENGTH ENDS + LEFT RIGHT + 48 689 6 2 + GEL LINES + NAME NUMBER POSITION LENGTH NEIGHBOURS + LEFT RIGHT + HINW.010 6 1 -279 0 3 + HINW.007 3 91 -265 6 5 + HINW.009 5 137 -299 3 17 + HINW.999 17 140 273 5 12 + HINW.017 12 193 265 17 18 + HINW.031 18 385 -245 12 2 + HINW.004 2 401 -289 18 0 + +.end lit +.left margin1 +@23. TX 3 @Complement a contig +.LEFT MARGIN2 +.PARA + This function will complement and reverse all of the gel +readings in a + contig. It automatically reverses and complements each gel + reading sequence, reorders left and right neighbours, recalculates +relative + positions and changes each strandedness. +.PARA + The only user input required is to identify the contig to + complement by the number or name of a gel reading it contains. +DO NOT KILL THE +PROGRAM DURING THIS STEP! +.left margin1 +@22. TX 3 @ Join contigs +.LEFT MARGIN2 +.PARA +This function joins contigs interactively using a mouse driven editor. +The operation of this editor is very similar to the Contig Editor +described in "Edit". + +.para +It allows the +user to align the ends of the two contigs by editing each +contig separately. It is important that the alignment achieved is +correct because once the join is completed the alignment is fixed. +The program needs to know which two contigs to join. +.para +First specify which two contigs are to be joined. +The user should identify the two +contigs. +The program checks that the two contig numbers are different (it will not +allow circles to be formed!) +.para +The Join Editor consists of two Contig Editors in between which is sandwiched +a disagreement box. This disagreement box shows exclamation marks to +denote mismatches between the two consensuses. +.para +For example, the display will look something like this: +.lit + + 1460 1470 1480 1490 1500 + 56 HINW.100 TCT*GAGCAGTGTGGGCGCTG*CCGG + 33 HINW.300 TCT*GAGCAGTGTGGGCGCTGC*CGGGCTCGGAGGG + -25 HINW.090 TCT*GAGCAGTGTGGGCG*T*G*CGGGCTCGGAGGG + 19 HINW.123 TCTCGAGCAGTGTGGGCGCTG**CGGGCTCGGAGGGCATGAAGTAGAGCG + CONSENSUS TCTCGAGCAGTGTGGGCGCTG-CCGGGCTCGGAGGGCATGAAGTAGAGCG + MISMATCH ! !!!!!! + 10 20 30 40 50 + -6 HINW.010 TCTCGAGCAGTGTGGGCGCTGCCCGGGCTCGGAGGGCATGAAGTTAGAGC + -3 HINW.007 TGGGCGCTGCCCGGGCTCGGAGGGCATGAAGT*AGAGC + -5 HINW.009 GCTCGGAGGGCATGAAGT*AGAGC + CONSENSUS TCTCGAGCAGTGTGGGCGCTGCCCGGGCTCGGAGGGCATGAAGTTAGAGC + +.END LIT +.para +The overlap must be of at least one character. +Use the scroll bar and the scroll buttons (`<<',`<',`>',and`>>') +for positioning the relative positions of the two contigs. +.para +The join position can be fixed in position +by pressing the `lock' button at the top of the Join Editor. +Locking allows the two contigs to be scrolled as one when using the scroll bar +and buttons, the left ends always in the same position relative to each +other. +.para +Once locked, it is best to proceed to the right along the contigs, inserting +padding characters (`*') into the consensuses to minimise the +disagreements. +.para +It is essential that the user aligns the two contigs throughout the whole +region of overlap before completing the join because it is only at this +stage that the two contigs can be edited independently. Once the join is +completed the alignment can only be altered using the routines supplied +by "alter relationships". +.para +The join can be completed by pressing the `Leave Editor' button. The +percentage mismatch is displayed, and the user is required to confirm that +they want to perform the join. +.left margin1 +@24. TX 1 @ Copy the database +.LEFT MARGIN2 +.PARA +Used to make a copy of the database. If required the database size can be + +altered using this option. The "version" of a database is encoded as the + +last letter in the names of the five files that contain the database. + +.para +Supply a "version" number (the default is version 1), and if required + +select a new size for the database. The size of a database is the number + of +lines of information it can hold. It needs a line for each gel reading and + +another for each contig. +.left margin1 +@19. TX 1 @ Check database +.LEFT MARGIN2 +.para +Used to perform a check on the logical consistency of the + database. No user intervention is required. If selected "with +dialogue" the program also checks for any sections of the consensus that +contain 15 dashes in 20 characters. +.para + The following relationships are checked: +.LEFT MARGIN2 + 1. If gel reading A thinks gel reading B is its left + neighbour + +does B think A is + its right neighbour? + The error message is +.left margin2 +"Hand holding problem for gel reading A" +.left margin2 +followed by the + gel descriptor lines for gel readings A and B. +.LEFT MARGIN2 + 2. Are there any contig lines with no left or right +end gel readings? + The error message is +.left margin2 +"Bad contig line number A" +.LEFT MARGIN2 + 3. Do the gel readings that are described as left ends on +contig + lines agree that they are left ends? + The error message is +.left margin2 +"The end gel readings of contig A have outward neighbours" +.LEFT MARGIN2 + 4. Are there gel readings that are in more than one contig? + The error message is +.left margin2 +" Gel number A is used N times" +.LEFT MARGIN2 + 5. Are there gel readings that are not in any contig? + The error message is +.left margin2 +" Gel number A is not used" +.LEFT MARGIN2 + 6. Do the relative positions of gel readings agree with +their + position as defined by left and right neighbourliness? + The error message is +.left margin2 +" Gel number A with position X is left neighbour of gel number B with +position Y" +.LEFT MARGIN2 + 7. Are there any loops in contigs? If so no further + checking is done. + The error message is +.left margin2 +" Loop in contig n no further checking done, but gel reading numbers follow" +.left margin2 + The + program then prints the gel reading numbers in the looped +contig up +to + the start of the loop. +.LEFT MARGIN2 +8. Are there any contigs of length <1? The error message is +.left margin2 +" The contig on line +number x has zero length" +.LEFT MARGIN2 +9. Are there any gel readings (used in only one contig) that have zero + +length? The error +message is +.left margin2 +" Gel number N has zero length" +.left margin2 +Note that "auto assemble" also uses this logical consistency check and + will +only tolerate a "Gel number N + is not used" error. Any other error will cause it to + +give up. + +.left margin1 +@29. TX 1 @ Examine quality +.LEFT MARGIN2 +.para +Analyses the quality of the data in a contig. It reports on the proportion + +of the consensus that is "well determined" and will display a sequence of + +symbols that indicate the quality of the consensus at each position. + +.para +Identify the contig to analyse, and the section of interest. The current + +consensus calculation cutoff score will be used to decide if each position +is +"well determined". In general the quality of a reading deteriorates along +the length of the gel and so it is also possible to use a length cutoff for +the quality calculation. Only the data from the first section of each reading +will be included in the quality calculation. The length is altered under +"set parameters" and is initially set to the maximum reading length. +A summary showing the percentage of the consensus +that falls into each category of quality is shown. Choose whether or not to +have the quality codes for each position of the consensus displayed. +They can be displayed as either graphics or text. +.para +The quality of the data depends on the number of times it has been + +sequenced and the particular uncertainty codes used in each gel + +reading. This function divides the data into five categories, assigning + +each +a symbol or code: +.LEFT MARGIN2 + 1. Well determined on both strands and they agree. code=0 +.LEFT MARGIN2 + 2. Well determined on the plus strand only. code=1 +.LEFT MARGIN2 + 3. Well determined on the minus strand only. code=2 +.LEFT MARGIN2 + 4. Not well determined on either strand. code=3 +.LEFT MARGIN2 + 5. Well determined on both strands but they disagree. code=4 +.LEFT MARGIN2 + A position is "well determined" if it is assigned one of the symbols +A,C,G,T when the algorithm described in the section "calculate a +consensus". +The calculation is performed +separately for each strand. +.para +If the user chooses to have the data displayed graphically the following +scheme is used. A rectangular box is drawn so that the x coordinate +represents the length of the contig. The box is notionally +divided vertically into +5 possible levels which are given the y values: -2,-1,0,1,2. +The quality codes attributed to each base position are plotted as +rectangles. +Each rectangle represents a region in +which the quality codes are identical, so a single base having a different +code from its immediate neighbours will appear as a very narrow rectangle. +.lit + + Rectangle bottom and top y values + + Quality 0 rectangle from 0 to 0 + Quality 1 rectangle from 0 to 1 + Quality 2 rectangle from 0 to -1 + Quality 3 rectangle from -1 to 1 + Quality 4 rectangle from -2 to 2 +.end lit +.para +Obviously a single line at the midheight shows a perfect sequence. +.para +Typical dialogue is shown below. +.lit + + 41.47% OK on both strands and they agree(0) + 55.48% OK on plus strand only(1) + 2.08% OK on minus strand only(2) + 0.97% Bad on both strands(3) + 0.00% OK on both strands but they disagree(4) + ? (y/n) (y) Show sequence of codes + + 10 20 30 40 50 + 1111111111 1111111111 1111111111 1111111111 1111111111 + + 60 70 80 90 100 + 1111111111 1111111111 1111111111 3111111111 1111111111 + + 110 120 130 140 150 + 1111111111 1111131111 1111111111 1111111111 1111111111 + + 160 170 180 190 200 + 1111111111 1111111111 1111111111 1111111111 1111111133 + + 210 220 230 240 250 + 1311111111 1111111111 1111111110 0000000000 0000220000 + + 260 270 280 290 300 + 0000000000 0020000000 2200000202 0002000000 0000222200 + +.end lit +.left margin1 +@26. TX 3 @ Alter relationships +.LEFT MARGIN2 +.para +Used to make what are normally illegal changes to the database. That is + +the normal checks are not done and any item in the database can be +changed independently of all others. Users need to know what they are + +doing because it is very easy to make a horrible mess. Always start by + +making a copy! +.para +By using the options here users can +move one section of a contig relative to another, break contigs, remove +contigs, remove gel readings, etc. To give flexibility most + of the commands do only one thing. This means that several commands +may +have to be executed to complete any change. +.para +The following options are offered: +.lit + + Cancel + Line change + Check logical consistency + Remove contig + Shift + Move gel reading + Rename gel reading + Break a contig + Remove a gel reading + Alter raw data parameters + +.end lit +.left margin2 +1. QUIT returns to the main options of BAP. +.left margin2 + +3. Line change +.left margin2 + allows the user to change the contents of any line in the + +file of relationships. The line is selected by number, the + program prints the current line and prompts for the new line. + +.left margin2 +4. Check logical consistency +.left margin2 +5. Remove a contig +.left margin2 +This function removes a contig and all its gel readings. The user specifies +any reading in the contig. +.left margin2 +6. Shift +.left margin2 + allows the user to change all the relative positions of a + set of neighbouring gel +readings by some fixed value, i.e. it will + shift related gel readings + either left or right. It can therefore + be used to change the alignment of the gel +readings in a contig. +It prompts for the number of the first gel +reading to + shift and then for the distance to move them (Note a + negative value will move the gel readings + left and a positive value + right). It then chains rightwards (ie follows right + neighbours) and shifts each gel +reading, in turn, up to the end + of the contig. (This means that only those gel readings + from the first + to shift to the rightmost are moved). It updates the length of + the contig accordingly. + +.left margin2 +7. Move gel reading +.left margin2 + is a function to renumber a gel reading. It moves all the information + about a gel +reading on to another line. The user must specify the +number + of the gel reading +to move and the number of the line to place it. It + takes care of all the relationships. Of course gel +readings must not be + moved to lines occupied by other gel +readings! + +.left margin2 +8. Rename gel reading +.left margin2 + is a function that is used to rename the archive names of + gel +readings in the database; it only changes the name in the .ARN + file of the database. + +.sk1 +.LEFT MARGIN2 +9. Break contig +.LEFT MARGIN2 +.PARA +Occasionally it is necessary to break a contig into two parts and this can be +achieved using this option. The program needs only the number of a gel +reading. This is the gel reading that will become a left end after the +break. That +is, the break is made between this gel +reading and its left neighbour. A new contig +line is created so ensure that there is sufficient space in the database. +.left margin2 +10. Removing gel readings from contigs +.left margin2 +.PARA +Gel +readings can be removed from contigs. If they are essential for holding the +contig together (ie are the only gel reading covering a particular region), +the program will create a new contig. +.sk1 +.LEFT MARGIN2 +11. Alter raw data parameters +.LEFT MARGIN2 +.PARA +Allows the user to edit the individual raw data parameters, such as +the left and right cutoff lengths and the name of the machine readable trace +file. +The user must specify the gel line to modify, and provide new values for +the length of the raw sequence including cutoff lengths, the left cutoff position, the length of the original working sequence, the machine type, and the name +of the raw data file, where these values change. +.left margin1 +@27. TX 1 @ Set display parameters +.LEFT MARGIN2 +.para +Used to redefine the parameters that control the cutoff employed by the + +consensus calculation and quality examiner, the maximum length of each +reading to include in the quality calculation, the line length used by + +the display function, the text window length used by the graphics +options, and the graphics window length used by the graphics options. +.para +The default cutoff score is 75%. The default line length is 50 characters. +For protein sequences the cutoff is always 100%. +.para +The text window used by the graphics options controls the amount of +sequence listed at the crosshair position. The graphics window controls the +"zoom" function. Both these windows are defined as the number of bases that +should be shown, to both left and right of the crosshair. +.left margin1 +@30. TX 3 @ Shuffle pads +.left margin2 +.para +One weakness of the alignment strategy used is that padding +characters are not always aligned by the assembly routine. This function +attempts to align padding characters using a very simply strategy. It +does not solve all pad alignment problems but is a useful first step during +cleaning-up operations. +.LEFT MARGIN1 +@10. TX 2 @Clear graphics +.LEFT MARGIN2 +.para + Clears graphics from the screen. +.left margin1 +@11. TX 2 @Clear text +.LEFT MARGIN1 +.para + Clears text from the screen. +.left margin1 +@12. TX 2 @Draw a ruler. +.LEFT MARGIN2 +.para +This option +allows the user to draw a ruler or scale along the x axis of the screen to +help identify the coordinates of points of interest. The user can define +the position of the first base to be marked (for example if the active +region is 1501 to 8000, the user might wish to mark every 1000th base +starting at either 1501 or 2000 - it depends if the user wishes to treat +the active region as an independent unit with its own numbering starting +at +its left edge, or as part of the whole sequence). The user can also define +the separation of the ticks on the scale and their height. If required the +labelling routine can be used to add numbers to the ticks. +.left margin1 +@14. TX 2 @Reposition plots +.LEFT MARGIN2 +.para +The positions of each of the plots is defined relative to a users drawing +board which has size 1-10,000 in x and 1-10,000 in y. +Plots for +each option are drawn in a window defined by x0,y0 and xlength,ylength. +Where x0,y0 is the position of the bottom left hand corner of the window, + and xlength is the width of the window and ylength the +height of the window. +.lit + --------------------------------------------------------- 10,000 + 1 1 + 1 -------------------------------------- ^ 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 1 1 ylength 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 -------------------------------------- v 1 + 1 x0,y0^ 1 + 1 <---------------xlength--------------> 1 + --------------------------------------------------------- 1 + 1 10,000 + +.end lit +All values are in drawing board units (i.e. 1-10,000, 1-10,000). +The default window positions are read from a file "ANALMARG" when the +program is started. Users can have their own file if required. +As all the plots start +at the same position in x and have the same width, x0 and xlength are the +same for all options. Generally users will only want to change the start +level of the window y0 and its height ylength. + This option +allows users to change window positions whilst running the program. +The routine prompts first for the number of the option that the users +wishes +to reposition; then for the y start and height; then for the x start and +length. Note that changes to the x values affect all options. If the user +types only carriage return for any value it will remain unchanged. +Note that, unlike all the other programs, the boxes used to contain +analytical results (eg plot quality) should not be made to overlap one +another, as the function of the crosshair routine depends on which box the +crosshair is in! +.LEFT MARGIN1 +@15. TX 2 @Label a diagram +.LEFT MARGIN2 +.para +This routine allows users to label any diagrams they have produced. They +are asked to type in a label. When the user types carriage return to finish +typing the label the cross-hair appears on the screen. The user can +position it anywhere on the screen. If the user types R (for right justify) +the label will be +written on the diagram with its right end at the cross-hair position. +If the user types L (for left justify) the label will be written on the +diagram with its left end at the cross hair position. +The +cross-hair will then immediately reappear. The user may put the same +label +on another part of the diagram as before or if he hits the space bar he +will be asked if he wishes to type in another label. +.para +Typical dialogue follows. +.lit +? Menu or option number=15 +Type label then drive cross hair to left or right end +of label position then hit "L" to write label left +justified or "R" to write label right justified or +the space bar to quit + + +? Label=delta gene + + missing graphics + +? Label= + +.end lit +.left margin1 +@16. TX 2 @Display a map +.LEFT MARGIN2 +.para +This is disabled! +.left margin1 +@7. TX 1 @Redirect output +.LEFT MARGIN2 +.para +Used to direct output that would normally appear on the screen to a file and +to create postscript output. +.para +Select redirection of either text or graphics, and +supply the name of the file that the output should be written to. +.para + The results from the next options selected will not appear on the screen +but will be written to the file. When option 7 is selected again +the file will be +closed and output will again appear on the screen. +.left margin1 +@13. TX 2 @Use crosshair +.left margin2 +.para +This option puts a steerable cross on the screen which the user +drives around +by using the arrow keys (or mouse). When the crosshair is +visible a number of options are available if the user types one of a +set of special keyboard characters. Any other characters will cause +an exit from the crosshair option. The special keys are: +.lit + + I = Identify the nearest gel reading + Z = Zoom in + Q = plot Quality + S = display the aligned Sequences at the crosshair position + N = list the Names and Numbers of the sequences at the crosshair +.end lit +.para +In order for any of these special keys to operate, the crosshair +must be in an appropriate display box, and the precise function of +the keys will also depend on which box the crosshair is in. +.para + If the +crosshair is in the "plot all contigs" box, Z will cause a new box to +appear showing all the readings for the nearest contig; Q will give +the same as Z but will also produce an extra box showing the +"quality" plot. +.para + If Z is hit in the "plot single contig" box, the contig will be zoomed +to the current graphics window size. The zoom will be roughly +centred on the crosshair position. Because of this it is possible to +step along a contig by repeatedly zooming with the crosshair near +to one end of the single contig display box. If I is hit the crosshair +must be close to a gel reading line. If Q is hit, the quality plot will +be produced for the region shown in the plot single contig box. In +all cases when the "plot all contigs" box is shown, a vertical line will +bisect the line the represents the relevant contig, at the current +position. +.para +If the crosshair is in the plot quality box only the character "s" will operate +as a special symbol. +.para +The number of bases shown in the N and S options is controlled by +the current graphics text window size, and the size of the zoom +window by the current graphics window size. Both are set by the +parameter setting function of the general menu. +.left margin1 +@33. TX 2 @Plot single contig +.left margin2 +.para +This option produces a schematic of a selected region of a single +contig by drawing a horizontal line to represent each of its gel +readings. The lines show the relative positions of each reading and +also their sense. The plot is divided vertically into two sections by +a line that is identified by an asterisk drawn at each end. All lines +that lie above this line represent readings that are in their original +sense, all lines below show readings that are in the +complementary sense to their original. By use of the crosshair +function the plot can be stepped through and examined in more +detail. See help on crosshair. +.left margin1 +@34. TX 2 @Plot all contigs +.left margin2 +.para +This option produces a schematic of all the contigs in a database. It +does this by drawing a horizontal line to represent each of them. +In order to show the ends of each contig it draws the lines for +contigs at alternate heights: the first at height one, the +second at height two, the third at height one, etc. The order of the +contigs in the display is the same as their order in the database. +By use of the crosshair function the plot can be stepped +through and examined in more detail. See help on crosshair. +.left margin1 +@31. TX 3 @ Disassemble readings +.left margin2 +.para +This function is used to remove a list of readings from a database, or +to create a new contig from a single reading moved from an existing contig. +This latter mode is useful for repositioning a reading in a repeat: +once separated it can be placed in the join editor and scrolled by the +other copies. +Removal of sets of readings works in two modes: +1. A set of adjacent readings in a +contig can be removed by the user naming the two end ones; or 2. A batch +of readings from any number of contigs can be defined by the user naming +a file containing a list of reading names. The program cleans up the +database by moving data to fill up any holes made in the files. +.para +For both modes of operation the program will ask for a file of file names. +If users create their own file (ie mode 2) each reading NAME must be on +a separate line. For mode 1 the user types the NAMES of the leftmost +and rightmost readings to be removed. They and all intervening readings +will be removed. Note that the routine operates on reading names - not +numbers. For both modes, if necessary, new contigs will be created. +.left margin1 +@35. TX 1 3 @Find internal joins +.left margin2 +.para +The purpose of this function is to use data already in the database to +find possible joins between contigs. +Joins may have been missed due to poor data or may have not been made +due to repeated sequences. Where appropriate, it may be +possible to find potential +joins by using the "unused data" derived from sequencing machines. +.left margin2 +For all overlaps found when the X version is used, + the contig editor (in join mode) will be +called up with the two contigs aligned. +.left margin2 +The database is checked for logical consistency. Supply a minimum initial +match length, a minimum alignment block, the maximum pads per sequence, +the maximum percent mismatch after alignment, the probe length. Choose +if clipped data is to be used, if so define the window size for finding good +data and the number of dashes allowed in the window. Processing will commence. +Most of these values are used in an identical way in the autoassemble +function. The others are defined below. +.left margin2 +The program strategy +.left margin2 +Take the first contig and calculate its consensus. If clipped data is being +used examine all readings that +are in the complementary orientation, and sufficiently near to the contigs left +end, to see if they have good clipped sequence which if present, would +protrude +from the left end of the contig. If found add the longest such sequence to the +left end of the consensus. Do the same for the right end by examining +readings that are in their +original orientation. If any are found add the longest extension to the +right end of +the consensus. Repeat the consensus calculations and extensions +for all contigs hence producing an extended consensus. If clipped data is not +being used simply calculate the consensus for the whole database. Now +look for possible joins by processing the extended consensus in the following +way. Take the last, say 100, bases (termed the "probe length" by the program) +of the rightmost consensus, compare it both +orientations with the extended consensus of all the other contigs. Display +any sufficiently good alignments. Repeat with the left end of the rightmost +contig. Do the same for the ends of all the entended contigs, always only +comparing with the contigs to their left, so that the same matches do not +appear twice. +.left margin2 +Good cliped data is defined by sliding a window of "Window size for good data +scan" bases outwards +along the sequence and stopping when "Maximum number of dashes in scan window" + or more dashes appear in the window. +Note that +it is advisable to have some sort of cutoff because if we simply take all the +data it might be so full of rubbish that we wont find any good matches. For +the same reason it is worth trying the procedure with different cutoffs. An +initial run using no clipped data is also recommended. +Sufficiently good +alignments are defined by criteria equivalent to those used in autoassemble, +however here we only display alignments that pass all tests. +.left margin2 +Bugs +.left margin2 +If a small contig is wholly contained within a larger one, such that its +ends are further than ("Probe length" - "Minimum initial match length") +from the ends of the larger contig, and the consensus for the small +contig lies to the left +of the consensus for large contig, the overlap will not be discovered. (See +the search stratgey). +.left margin2 + All numbering is +relative to base number one in the contig: matches to the left (i.e. in +the clipped data) have negative +positions, matches off the right end of the contig (i.e. in the clipped +data) have positions +greater than that of the contig length. +The convention for reporting the positions of overlaps is as follows: if neither +contig needs to be complemented the positions are as shown. If the program says +"contig x in the - sense" then the positions shown assume contig x has been +complemented. For example in the results given below the positions for the +first overlap are as reported, but those for the second assume that the contig +in the minus sense (i.e. 443) has been complemented. +.lit + + + Possible join between contig 445 in the + sense and contig 405 + Percentage mismatch after alignment = 4.9 + 412 422 432 442 452 462 + 405 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAG,TT AGCTCACTCA + ********* * ******** ***** *** ********** ********** ********** + 445 -TTCCCGACT G,AAAGCGGG TAGTGA,CGC AACGCAATTA ATGTGAG-TT AGCTCACTCA + -127 -117 -107 -97 -87 -77 + 472 482 492 502 512 + 405 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT AT + ********** ********** ********** ********** ** + 445 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT AT + -67 -57 -47 -37 -27 + Possible join between contig 443 in the - sense and contig 423 + Percentage mismatch after alignment = 10.4 + 64 74 84 94 104 114 + 423 ATCGAAGAAA GAAAAGGAGG AGAAGATGAT TTTAAAAATG AAACG-CGAT GTCAGATGGG + **** ***** ********** ********** ****** ** ***** **** ********* + 443 ATCG,AGAAA GAAAAGGAGG AGAAGATGAT TTTAAA,,TG AAACGACGAT GTCAGATGG, + 3610 3620 3630 3640 3650 3660 + 124 134 144 154 164 + 423 TTG-ATGAAG TAGAAGTAGG AG-AGGTGGA AGAGAAGAGA GTGGGA + *** ****** ********** ** ******* *** ***** ** ** + 443 TTGGATGAAG TAGAAGTAGG AGGAGGTGGA ,GAG,AGAGA GTTGG- + 3670 3680 3690 3700 3710 + + +.end lit +.left margin1 +@36. TX 3 @Double strand +.left margin2 +.para +PLEASE MAKE A COPY OF THE DATABASE BEFORE USING THIS OPTION AS IT HAS +CURRENTLY HAD VERY LITTLE TESTING. +.para +Uses the cutoff data to change single stranded sections of a contig into +double stranded sections. Data is used carefully to try and minimise the +number of data disagreements created. However it must be noted that an overall +slight degradation in quality will still occur. +.para +When using this option you will be prompted for a contig and a region within +that contig. The default region is the entire contig. The option will then +search through the region for areas of good data on one strand and cutoff data +on the opposite strand, extending the cutoff data. The criteria for evaluating +the amount of cutoff data to be used is based upon a maximum number of +mismatches and a score (derived by accumulating points for mismatches (-8), +matches(+1) and insertions (-5) over the length of an alignment). The defaults +are: +.lit + +maximum mismatches : 6 + +score for mismatch : -8 +score for correct match : +1 +score for insertion : -5 +.end lit +.para +Note that with successive calls to this option it is possible to double strand +more and more data. Naturally however the quality of the data generated will +diminish each time. +.left margin1 +@37. TX 3 @Auto-select oligos +.left margin2 +.para +PLEASE MAKE A COPY OF THE DATABASE BEFORE USING THIS OPTION AS IT HAS +CURRENTLY HAD VERY LITTLE TESTING. +.para +Generates a file (default "primers") of suggested primers to use for covering +a single stranded section or for walking off the end of a contig. The file +generated contains the gel reading name, the primer sequence, it's offset in +the contig and the orientation. An example file would be : +.lit + +c81d12.s1 TTGTCTGTAAGCGGATG (@ 6449 ) + +c98a10.s1 ATTATCACTTTACGGGTC (@ 6959 ) + +c81c1.s1 CAAGAAGGCGATAGAAG (@ 7643 ) + +c76a10.s1 CCTCATCCTGTCTCTTG (@ 8441 ) + +c81g4.s1 ATGAAACCTGGGCGTTG (@ 16156 ) + +c91e6.s1 GTTTTCAGATGTCGGAG (@ 18249 ) + +c81e12.s1 GCTACCGTAAAACACTTC (@ 18737 ) + +c93h11.s1 GCTGCTTTTTGTTTTATCC (@ 19158 ) + +c81h6.s1 CTTCCACTTCTTTCTTATC (@ 21210 ) + +c86a12.s1 CGAATGATAAAGACAAATCAG (@ 22122 ) + +c98b1.s1 GCCACTTTATCCGAGAC (@ 3048 ) - +c97c5.s1 GTGTTTTGGGTATATTGTG (@ 3371 ) - +c83d2.s1 CTACACAGAATGAACCC (@ 3768 ) - +c78h10.s1 GGCGGTGAAGATTGAAG (@ 4200 ) - +c98h9.s2dt CTCGTTTAAATTTCAAACTTCC (@ 7419 ) - +c95a9.s1 ATTGGAAGGAAGGAGGG (@ 22996 ) - +c82b4.s1 TGTAGCCGAAATCTTCC (@ 23369 ) - +.end lit +.para +This is best employed after having previously used the 'Double strand' option. +When selecting the option you will be asked for the contig, a region within +this contig and the file to write the list of primers to. For each primer +suggested a tag is automatically created containing details of the gel reading +name and the sequence. Preferably the tag will be created on the gel reading +from which the primer was selected. However this is not always possible so +failing that the tag will be on another sequence overlapping the primer +position. +.para +When invoked with the dialogue option you will be asked a couple more +questions relating to the position and size of the consensus checked for +suitable oligos. You will be prompted for the start and end of a region +(default 40-140) at a relative position to the left of our initial region. +.para +For example: +.lit + +? Menu or option number=d37 + Auto-select oligos + Default Contig identfier=/e97f2.s1 + ? Contig identfier= + ? Start position in contig (1-20942) (1) =10000 + ? End position in contig (10000-20942) (20942) =11000 + Default Name of file for primers=primers + ? Name of file for primers= + ? Start of oligo choice region (1-1024) (40) =50 + ? End of oligo choice region (50-1024) (150) =150 + +.end lit +.para +This implies that we are going to look for oligos to use as primers covering +the region 10000 to 11000. For each single stranded section in this region we +search for the oligos at between 50 and 150 to the left. So if we had a single +stranded section from 10121 to 10295 we would search for oligos in the region +9971 to 10071. +.left margin1 +@38. TX 1 @Check assembly +.left margin2 +.para +This new function is used for checking the positioning of assembled readings. +It is useful for checking sequences that contain repeats +of length similar to that of a single gel reading. It takes the poor +quality data for each reading and compares it to the segment of the consensus +to which it should align. +If the extension of the +read does not match the consensus then the read (or its neighbours) has +probably been assembled into the wrong place. +The program displays the bad alignments. +The quality of an alignment is defined by the percentage mismatch. +Naturally the user should select a value that takes into account +the poor quality of the data being aligned. +.para +When the routine is used from the X version the +user is offered the editor to examine poor alignments. + If alignments are reported as poor, but on inspection are OK, the user +can set a tag so that the poor quality data is ignored on subsequent passes +through the routine. Note however such data will then also be ignored by +the automatic double stranding routine! +.para + The user defines the percentage mismatch; the window size and number of +dashes allowed in the window used for selecting the amount of the poor data +to be employed; can choose to save the names of the poorly aligned reads +in a file; can select an individual contig or scan the whole database. +The file containing the names of the poorly aligned reads can be used by +the disassembly routine to remove them from the database, and then can be used +to reassemble them. Note that the routine complements each contig twice +during processing. + +.left margin1 +@39. TX 1 @Find read pairs +.left margin2 +.para +This new function is used to check the positions of readings taken from each +end of the same template. For each forward read it searches for a corresponding +reverse reading. The search can be over the whole database or over a single contig. +The results can be presented graphically for single contig searches and the crosshair +function can be used to identify the readings displayed. +.para +Note that at present the function only knows that two reads are from the same template +by comparing reading names. For our local projects we use the following naming +convention: forward reads are named abcdefgh.s1 and reverse reads abcdefgh.r1. The +program expects this naming convention and so if it finds read fred.s1 and fred.r1 it +assumes they are the forward and reverse reads for template fred. In the future we +will make the routine more general! +.para +If a single contig is selected and the output is listed the program displays two +lines for each pair: the first line shows the reading name, its position and length, +and the distance between the extremeties of the two reads; the second line shows the +other read name, its position and length. If there are pairs that are in separate contigs +or are facing away from one another they are listed after the pairs that face inwards. +Is this true? +.para +If the results are plotted the full length of the template is drawn with arrows +indicating the direction of reads and the extent of each reading. Those reads that have +their partner in another contig are marked by asterisks. +.para +Typical dialogue is shown below. +.lit + + ? Select contigs (y/n) (y) = + Default Contig identifier=/i55d8.s1 + ? Contig identifier= + ? Start position in contig (1-15227) (1) = + ? End position in contig (1-15227) (15227) = + ? Plot results (y/n) (y) = n + 852 k23a1.r1 249 238 1615 + 806 k23a1.s1 1529 -335 + 238 i68e6.s1 422 193 1632 + 868 i68e6.r1 1756 -298 + 576 k17a2.s1 2370 213 1676 + 885 k17a2.r1 3790 -256 + 84 k27g6.s1 3456 291 1777 + 867 k27g6.r1 4905 -328 + 453 k01g10.s1 5805 142 1251 + 881 k01g10.r1 6909 -147 + 781 i98b8.r1 6754 338 1079 + 10 i98b8.s1 7653 -180 + 883 k02d11.r1 7327 276 1597 + 283 k02d11.s1 8726 -198 + 269 i68f9.s1 8191 169 1055 + 777 i68f9.r1 8891 -355 + 710 i91c6.s1 8245 95 1516 + 780 i91c6.r1 9403 -358 + 596 k27d12.s1 136 329 -329 + 219 k27d12.r1 1 -116 + 159 k27d11.r1 1830 -263 -263 + 317 k27d11.s1 2902 343 + 886 k17g11.r1 7107 -123 -123 + 647 k17g11.s1 1867 265 + 851 i69g10.r1 8045 -137 -137 + 277 i69g10.s1 4658 174 +.end lit +.para +If contigs are not selected the pairs are sorted on their separations. +.lit + + ? Select contigs (y/n) (y) = n + i68f2.s1 27 1781 1777 + i68f2.r1 776 111 1777 + k17f6.s1 601 60 1706 + k17f6.r1 856 1405 1706 + k17a2.s1 576 2370 1676 + k17a2.r1 885 3790 1676 + k27g3.s1 177 14985 1664 + k27g3.r1 889 13564 1664 +. +. + k27b12.s1 764 1 1086 + k27b12.r1 857 932 1086 + i98b8.s1 10 7653 1079 + i98b8.r1 781 6754 1079 + k16a3.s1 748 1276 1070 + k16a3.r1 784 472 1070 + k17b7.r1 786 14937 18942* + k17b7.s1 787 3601 18942* + k27d12.r1 219 1 15208* + k27d12.s1 596 136 15208* + k01g2.s1 502 87 14754* + k01g2.r1 782 9224 14754* + +.end lit + +.left margin1 +@ end of help diff --git a/help/DAP.RNO b/help/DAP.RNO new file mode 100644 index 0000000..7bcfa2d --- /dev/null +++ b/help/DAP.RNO @@ -0,0 +1,2724 @@ +.npa +.left margin1 +@-1. TX 0 @General +.sp +@-2. T 0 @Screen control +.sp +@-2. X 0 @Screen +.sp +@-3. TX 0 @Modification +.sp +@0. TX -1 @SAP +.left margin2 +.PARA +This is help information for the X Windows version of SAP. +Currently it is being brought up to date with the new features in XDAP. +The accuracy of this help should therefore not be assumed. +.PARA +This is an interactive program whose primary use is +for managing shotgun sequencing projects, but it can also be used for +handling alignments of other sequences, including those of proteins. +Currently the maximum 'gel reading' length is set to 4096 characters. +Almost all of the information below describes the use of the program for +shotgun projects, but those using the programs for handling other +sequence +alignments should interpret it accordingly. +The data for such a project is stored in a special type of database. The +program + contains the tools that are required to type in gel readings, +screen them against vector sequences and restriction sites; +enter new gel +readings into the database (automatically comparing and aligning +them). In addition it contains editors and functions to examine the quality +of the aligned sequences. +.para + There are three main menus: "general", "screen" and "modification", +and some functions have submenus. +.left margin2 +.lit + The general menu contains the following options: + + Open a database + Display a contig + List a text file + Direct output + Calculate a consensus + Screen against restriction enzymes + Screen against vector + Check database + Copy database + Show relationships + set parameters + Highlight disagreements + Examine quality + Find internal joins + +The graphics menu contains: + + Clear graphics + Clear text + Draw ruler + Use cross hair + Change margins + Label diagram + Plot map + Plot single contig + Plot all contigs + + +The modification menu contains: + + Edit contig + Auto assemble + Join contigs + Complement a contig + Alter relationships + Extract gel readings + + +The alter relationships menu contains: + + Cancel + Line change + Edit single gel reading + Delete contig + Shift + Move gel reading + Rename gel reading + Break contig + Alter raw data parameters + +.END LIT +.SK1 +.para +Overview of the methodology +.para +The shotgun sequencing strategy +.para + In the shotgun sequencing procedure +the sequence to be determined is randomly broken into fragments of +about +400 nucleotides in length. These fragments are cloned and then +selected randomly and their + + sequences determined. The relationship between any pair of + + fragments is not known beforehand +but is found by comparing their sequences. + + If the sequence of one found to be wholly or partially contained + + within that of another for sufficient length to distinguish an + + overlap from a repeat then those two fragments can be joined. +The + + process of select, sequence and compare is continued until the +whole + + of the DNA to be sequenced is in one continuous well +determined + + piece. + +.para + Definition of a contig + +.para + A CONTIG is a set of gel readings that are related to one + another by overlap of their sequences. All gel readings belong to + a contig and each contig contains at least one gel + reading. The gel readings in a contig can be summed to produce +a continuous consensus sequence and the length of this sequence is +the length of the contig. The rules used to perform this summation are + given under "the consensus algorithm". + At any stage + of a sequencing project the data will comprise a number of +contigs; +when a project is + + complete there should be only one contig and its consensus will be + the finished sequence. Note that since being introduced and +defined as above the word "contig" has been taken up by those involved in +genomic mapping. In that context the consensus with a precise length is not +defined. + +.SK1 +.LEFT MARGIN2 +Introduction to the computer method +.LEFT margin2 +.PARA +It is useful to consider the objectives of a sequencing project before +outlining how we use the computer to help achieve them. The aim of a +shotgun sequencing project is to +produce an accurate consensus sequence from many overlapping gel +readings. +It is necessary to know, particularly at the latter +stages of the project, how accurate the +consensus sequence is. This enables us to know which regions of the + sequence require further work and also to know when the project is +finished. +To show the quality of the consensus, the programs described here +produce displays like that shown below. +.sk1 +.lit + + 10 20 30 40 50 + -6 HINW.010 GCGACGGTCTCGGCACAAAGCCGCTGCGGCGCACCTACCCTTCTCTTATA + CONSENSUS GCGACGGTCTCGGCACAAAGCCGCTGCGGCGCACCTACCCTTCTCTTATA + + 60 70 80 90 100 + -6 HINW.010 CACAAGCGAGCGAGTGGGGCACGGTGACGTGGTCACGCCGCGGACACGTC + -3 HINW.007 GGCACA*GTC + CONSENSUS CACAAGCGAGCGAGTGGGGCACGGTGACGTGGTCACGCCG-G-ACA-GTC + + 110 120 130 140 150 + -6 HINW.010 GATTAGGAGACGAACTGGGGCG3CGCC*GCTGCTGTGGCAGCGACCGTCG + -3 HINW.007 GATTAG4AGACGAACTGGGGCGACGCCCG*TGCTGTGGCAGCGACCGTCG + -5 HINW.009 GGCAGCGACCGTCG + 17 HINW.999 AGCGACCGTCG + CONSENSUS GATTAGGAGACGAACTGGGGCGACGCC-G-TGCTGTGGCAGCGACCGTCG + + 160 170 180 190 200 + -6 HINW.010 TCT*GAGCAGTGTGGGCGCTG*CCGGGCTCGGAGGGCATGAAGTAGAGC* + -3 HINW.007 TCT*GAGCAGTGTGGGCGCTGC*CGGGCTCGGAGGGCATGAAGTAGAGC* + -5 HINW.009 TCT*GAGCAGTGTGGGCG*T*G*CGGGCTCGGAGGGCATGAAGTAGAGC* + 17 HINW.999 TCTCGAGCAGTGTGGGCGCTG**CGGGCTCGGAGGGCATGAAGTAGAGCG + 12 HINW.017 GTAGAGC* + CONSENSUS TCT*GAGCAGTGTGGGCGCTG-*CGGGCTCGGAGGGCATGAAGTAGAGC* +.END LIT +.para + This is an example showing the left end of a contig from + position 1 to 200. Overlapping this region are gel readings +numbered 6, 3, 5, 17 and 12; +6, 3 and 5 +are in reverse orientation to their original reading (denoted by a minus +sign). Each gel reading also has a name (eg HINW.010). It can be seen that +in a number of places the sequences contain characters other than A,C,G +and +T. Some of these extra characters have been used by the sequencer to +indicate regions of uncertainty in the initial interpretation of the gel +reading, but the asterisks (*) have been inserted by the automatic +assembly function in order to align the sequences. Underneath each 50 +character block of gel reading sequences is the consensus derived from +the +sequences aligned above (the line labelled CONSENSUS). For most of its +length the consensus has a definite nucleotide assignment but in a few +positions there is insufficient agreement between the gel readings and +so a dash (-) appears in the sequence. This display contains all the +evidence needed to assess the quality of the consensus: the number of +times +the sequence has been determined on each strand of the DNA, and the +individual nucleotide assignments given for each gel reading. +.para +So the aim is to produce the consensus sequence and, equally important, +a display of the experimental results from which it was derived. +.para +In order to achieve this the following operations need to be performed: +.left margin2 +1) Put individual gel readings into the computer. +This might involved the manual interpretation of autoradiographs +or the transfer and process of machine-readable files from fluorescent +sequencing machines. +.left margin2 +2) Check each gel reading to make sure it is not simply part of one of the +vectors used to clone the sequence. +.left margin2 +3) Check each gel reading to make sure that those fragments that span +the +ligation point used prior to sonication are not assembled as single +sequences. +.left margin2 +4) Compare all the remaining gel readings with one another to assemble +them +to produce the consensus sequence. +.left margin2 +5) Check the quality of the consensus and edit the sequences. +.left margin2 +6) When all the consensus is sufficiently well determined, produce a copy +of +it for processing by other analysis programs. +.para +It is very unlikely that this procedure will only be passed through once. +Usually steps 1 to 5 are cycled through repeatedly, with step 4 just +adding +new sequences to those already assembled. Generally step 6 is also used +in +order to analyse imperfect sequence to check if it is the one the project +intended to sequence, or to look for interesting features. Analysis of +the consensus, such as +searches for protein coding regions, +can also help to find errors in the sequence. The display of the +overlapping gel readings shown above can be used to indicate, not only +the +poorly determined regions, but also which clones should be resequenced +to +resolve ambiguities, or those which can usefully be extended or +sequenced +in the reverse direction, to cover +difficult regions. + +.PARA +The original +individual gel readings for a sequencing project are each stored in +separate files. As the gel readings are entered into the computer +(usually in batches, say 10 +from a film), the file names they are given are stored in +a further file, called a file of file names. Files of file names +enable gel readings to be processed in batches. +.para +For each sequencing project +we start a project database. This database has a structure specifically +designed for +dealing with shotgun sequence data. +In order to arrive at the final consensus sequence many operations will +be +performed on the sequence data. Individual fragments must be +sequenced and +compared in both senses (i.e. both orientations) with all the other +sequences. When an overlap between a new gel reading and a contig are +found +they must be aligned and the new gel reading added to the contig. If a +new +gel reading overlaps two contigs they must be aligned and joined. Before +the two contigs are joined one of them may need to be turned around +(reversed and complemented) so they are both in in the same orientation. +.para +Clearly, keeping track of all these manipulations is quite complicated, +and to be able to perform the operations +quickly requires careful choice of data +structure and algorithms. For these reasons it is not practicable to store +the gel readings aligned as shown in the display above. Rather, it is more +convenient to store the sequences unassembled, and to record sufficient +information for programs to assemble them during processing. The +data used to assemble the sequences is called relational information. +.left margin2 +.PARA + The database comprises five files and they are described under the +section entitled "open database". +.PARA +Before entry into the project database +each new gel reading must be compared to look for overlaps +with all the data already contained +within the database. This last point is +important: all searching for overlaps is between individual new gel +readings and the data already in the database. There is no searching for +overlaps between sequences within the database; overlaps must be found +before new gel readings are entered into the database. +.para +Below I give an introduction to how the sequences are processed by +being +passed from one function to the next. +.para +This program is used to start a +database for the project and +then the following procedure is used. +.para +Data in the form of individual gel readings are entered into the computer + +and stored in separate files using either program this program or the digitizer + +program. Batches +of these gel readings +are passed to the screening functions in this program to search for overlaps + +with vector sequences ("screen against vector") or for matches to + +restriction enzyme sites that should not be + +present ("screen against enzymes"). +Each run of these screening functions passes on only those gel + +readings that do not contain unwanted sequences. Sequences are passed + +via +files of file names and eventually are processed by the automatic +assembly function ("auto assemble"). This function compares each gel +reading with a consensus of all the previous gel readings +stored in the database. +If it finds any +overlaps + it aligns the overlapping sequences by inserting padding characters, +and then adds the new gel reading to the database. +Gels that overlap are added to existing contigs and gels that do not +overlap any data in the database start +new contigs. If a new gel overlaps two contigs they are joined. +Any gel readings that appear to overlap but which +cannot be aligned sufficiently well are not entered and have +their names written to a file of failed gel reading names. +.PARA +Generally data is entered +into the database in batches as just described. The program + is also used to examine + +the data in the database, to enter gel readings that the automatic + +assembly function cannot align ("auto assemble"), + + and to make final edits. Edits to whole contigs + +can be made in several ways. +A mouse-driven editor ("edit contig") is used to perform all edits manually. +Disagreements between gel readings + +in contigs and their consensus + +sequences can be highlighted by use of the function "highlight + +disagreements". +.PARA +Editing the sequences is obviously an essential part of managing a + +sequencing project. +Editing is required when new + +sequences are added, when contigs are joined, and when sequences are + +corrected. +A basic part of the strategy + +used here is that new + +gel readings should be correctly aligned throughout their whole length + +when +they are entered into the database, and that when contigs are joined they + +are edited so that they are well aligned in the region of overlap. + + Alignment can be achieved by + +adding padding characters to the sequences, and this is the way "auto + +assemble" +operates when adding new sequences to the database. + +.para +In order to search +for overlaps that may have been missed due to errors in + +the gel readings, the function "extract gel readings" can be used to take + +copies of the gel + +readings at the ends of contigs, and write them out as separate files. + +These can then be compared with the database consensus using the "auto + +assemble" function in a mode that forbids entry of data into the +database, +and any gel reading matching two contigs will indicate a join that has + +been +missed. The joins can then be made interactively using "join contigs". + +Missed matches can be + +found at this stage because the errors in the sequences may have been + +corrected by new data. + +.para +Generally the users need not concern themselves with how the relational +information is used by the program, but it is necessary to know +how contigs are identified. Because contigs are constantly being changed and +reordered the program identifies them by the numbers of the gel readings +they contain. Whenever users need to identify a contig they need only +know +the number or name of one of the gel readings it contains. Whenever the +program asks users to identify a contig or gel reading they can type its +number or its archive name. If they type its archive name they must precede +the name by a slash "/" symbol to denote that it is a name rather than a +number. E.g if the archive +name is fred.gel with number 99, users should +type /fred.gel or 99 when asked to identify the contig. Generally, + when it asks for the gel reading to be identified, +the program will offer the user a default name, + and if the user types only return, that +contig will be accessed. When a database is opened the default contig will +be the longest one, but if another is accessed, it will subsequently become +the current default. +.para +Further information is located in the following places. +The database files are described under "open database". The format +for +vector and consensus sequences is given under "calculate a consensus", as are +the +uncertainty codes used in gel readings. +.left margin2 +.para +There are two programs, +other than this, relevant to sequencing are the digitizer +program and the trace editor program, both is outlined briefly below. +.para + The digitiser program +is used for the initial input of gel readings +and for writing a file of file names. The program +uses a digitizer for data entry. +A digitizer is + a two dimensional surface such as a light box +which is such that if a special pen is pressed onto it, the pens +coordinates are recorded by a computer. +These coordinates + can be interpreted by a program. +.para + In order to read an autoradiograph placed on the light box +the user need only define the bottom of +the four sequencing lanes and the bases + to which they correspond and then use the pen to point to each + successive band progressing up the gel. The program examines +the + coordinates of each pen position to see in which of the four +lanes + it lies and assigns the corresponding base to be stored in the + computer. Each time the pen tip is depressed to point to a position + on the surface of the digitizer the program sounds the bell on the + terminal to indicate to the user that a point has been recorded. As + the sequence is read the program displays it on the screen. +.para + The trace editor program +is used for the initial processing of data obtained from +fluorescent sequencing machines. It allows the user to visually +select left and right cutoff positions to denote the start and end of good +data. Users may also edit the sequence at this point. +Output from ted is a sequence file in Staden format with headers that +describe to xdap the cutoff information. + +.left margin1 +@17. TX 1 @Screen against enzymes +.left margin2 +.PARA +Used to compare gel readings against any restriction enzyme recognition + +sequences that may have been used during cloning and which should not + +be present in the data. Works on single gel readings or processes batches + +accessed through files of file names. The algorithm looks for exact + +matches to recognition sequences stored in a file. + +.para +The file containing the recognition sequences must be identified. The +user +must choose between employing a file of file names, or typing in the + + +names of individual gel reading files. If a file of file names is used the + + +program will also create a new file of file names. When the option has + +finished operating this new file will contain the names of all those gel + +readings that did not match any of the recognition sequences. Hence it + can +be used for further processing of the batch. The recognition sequences + +should be stored in a simple text file with one recognition sequence per + +line. +.left margin1 +@18. TX 1 @Screen against vector +.left margin2 +.PARA +Used to compare gel readings against any vector sequences that may have + +been picked up during cloning. Works on single gel readings or processes + +batches accessed through files of file names. The algorithm looks for +exact +matches of length "minimum match length" and displays the overlapping + +sequences. +.para +The file containing the vector sequence must be identified. The user must + +choose between employing a file of file names, or typing in the names of + +individual gel reading files. If a file of file names is used the program +will +also create a new file of file names. When the option has finished + +operating this new file will contain the names of all those gel readings + +that did not match the vector sequence. Hence it can be used for further + +processing of the batch. The vector sequence should be stored in a simple + +text file with up to 80 characters of data per line. More than one vector + +can be stored in a single file. If so each should be preceded by a 20 + +character title of the form <---m13mp8.001-----> where the < and > + signs +and the number like .001 are obligatory. The number must be preceded + +by a dot (.) and be 3 digits long. The total sequence in the file must be < + +50,001 characters in length. + +.left margin1 +@20. TX 3 @Auto assemble +.left margin2 +.PARA +Compares gel readings against the current contents of the database and + +produces alignments. In its normal mode of operation +("entry permitted"), the function +will automatically enter the gel readings into the database, but if entry +is not permitted it will only produce alignments. It works on + +single gel readings or processes batches of gel readings accessed through + +files of file names. It is the usual way to enter data into the database. + +.para +The function will check the database for logical consistency and will + only +proceed if it is OK. Choose if gel readings should be entered into the + +database, or if they should only be compared. Choose between using a file + +of file names or typing file names on the keyboard. If so selected, supply + +the file of file names. Also supply a file of file names to contain the names of + +all the gel readings that fail to get entered. +Select the entry mode. Normal assembly is appropriate for all but special +cases, as is "permit joins". Uses for the other modes are not documented +here. +Define a minimum initial + +match length. Define a minimum alignment block (the default value is + +taken in all but exceptional circumstances). Define the maximum number + +of padding characters allowed to be used in each gel reading to help + +achieve alignment, and the same for the number allowed in the contig for + +each gel reading. Finally define the maximum percentage mismatch to +be allowed for any gel reading to be entered into the database. If + +for any gel reading, either of these last three values is exceeded the gel + +reading will not be entered into the database. + +.para +In operation the function takes a batch of gel readings (probably passed + + on as a file of file names from one of the screening routines) and +enters them into a + database for a sequencing project. It takes each gel reading + in turn, + compares it with the current consensus for the database, it then + produces an alignment for any regions of the consensus it + overlaps; if this alignment is sufficiently good it then edits + both the new gel reading and the sequences it overlaps and adds +the + new gel reading to the database. The program then updates the +consensus + accordingly and carries on to the next gel reading. +.para + All alignments are displayed and any gel readings +that do match but that + + cannot be aligned sufficiently well have their names written to a + file of failed gel reading names. The function works without any + + user intervention and can process any number of gel readings in a + single run. Those gel readings that fail can be recompared using + + the same function (to find the current overlap position) and the + +user can enter them into the database + + manually using the "enter new gel reading" option. +.para +Typical dialogue and output from the function is shown below. (Note that +output for gel readings 2 - 9 has been deleted to save space). +.lit +Automatic sequence assembler +Database is logically consistent +? (y/n) (y) Permit entry +? (y/n) (y) Use file of file names +? File of gel reading names=demo.nam +? File for names of failures=demo.fail +Select entry mode +X 1 Perform normal shotgun assembly + 2 Put all sequences in one contig + 3 Put all sequences in new contigs +? Selection (1-3) (1) = +? (y/n) (y) Permit joins +? Minimum initial match (12-4097) (15) = +? Minimum alignment block (2-5) (3) = +? Maximum pads per gel (0-25) (8) = +? Maximum pads per gel in contig (0-25) (8) = +? Maximum percent mismatch after alignment (0.00-15.00) (8.00) = + >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> + Processing 1 in batch + Gel reading name=HINW.004 + Gel reading length= 283 + Searching for overlaps + Strand 1 + Strand 2 + No matches found + Total matches found 1 + Padding in contig= 0 and in gel= 1 + Percentage mismatch after alignment = 1.8 + Best alignment found + 1 11 21 31 41 51 + TTTTCCAGCG TGCGTCTGAC GCTGTCTTGC TTAATGATCT CCATCGTGTG CCTAGGTCTG + ********** ********** ********** ********** ********** ********** + TTTTCCAGCG TGCGTCTGAC GCTGTCTTGC TTAATGATCT CCATCGTGTG CCTAGGTCTG + 1 11 21 31 41 51 + 61 71 81 91 101 111 + TTGCGTTGGG CCGAGCCCAA CTTTCCCAAA AACGTATGGA TCTTACTGAC GTACA-GTTG + ********** ********** ********** ********** ********** ***** **** + TTGCGTTGGG CCGAGCCCAA CTTTCCCAAA AACGTATGGA TCTTACTGAC GTACACGTTG + 61 71 81 91 101 111 + 121 131 141 151 161 171 + CTTACCAGCG TGGCTGTCAC GGCGTCAGGC TTCCACTTTA GTCATCGTTC AGTCATTTAT + ********** ********** ********** ********** ********** ********** + CTTACCAGCG TGGCTGTCAC GGCGTCAGGC TTCCACTTTA GTCATCGTTC AGTCATTTAT + 121 131 141 151 161 171 + 181 191 201 211 221 231 + GCCATGGTGG CCACAGTGAC G-TATTTTGT TTCCTCACGC TCGCTACGTA TCTGTTTGCC + ********** ********** * ******** ********** ********** ********** + GCCATGGTGG CCACAGTGAC GCTATTTTGT TTCCTCACGC TCGCTACGTA TCTGTTTGCC + 181 191 201 211 221 231 + 241 251 261 271 281 + CGCG--GTGG AATTACAGCG TTCCCTATTG ACGGGCGCAT CCAC + **** **** ********** ** * ***** ********** **** + CGCGACGTGG AATTACAGCG TT,CDTATTG ACGGGCGCAT CCAC + 241 251 261 271 281 + Batch finished + 9 sequences processed + 0 sequences entered into database + 0 joins made + +.end lit + +.para +Note that "auto assemble" cannot align protein sequences. +.left margin1 +@28. TX 1 @Highlight disagreements +.left margin2 +.para +Used in the latter stages of a project +to highlight disagreements between individual gel readings +and their consensus sequences. Characters that agree with the + +consensus are shown as : symbols for the plus strand and . for the minus + +strand. Characters that disagree with the consensus are left unchanged + +and so stand out clearly. The results of this analysis are written to a +file. + +.para +Before selecting this option create a file of the display of the contig to +be +"highlighted". The option will ask for the name of this file. Select + symbols +to denote "agreeing" characters on each strand, the defaults are : and ., + +but any others can be used. Supply the name of a file in which to put + +the output. +.para +The display file needed as input for this option is created by selecting + +"Redirect output", followed immediately by "display contig", and then +"Redirect output" again. The + +cutoff score used in the consensus calculation can be set by option "set + +display parameters". Note that for the highlight function +there is a limit of 50 for the number of gel +readings that are aligned at any position - ie the contig must be less +than 51 gel readings deep at its thickest point. I hope that those performing +shotgun sequencing never reach this limit, but those using the program for +comparing sequence families might. +.para +Typical output from this function is shown below. +.lit + + 210 220 230 240 250 + 1 HINW.004 :C::::::::::::::::::::::::::::::::::::::::::AC:::: + 7 HINW.018 :*::::::::::::::::::::::::::::::::::::::::::CA:::: + -4 HINW.017 ...............AC.... + G-TATTTTGTTTCCTCACGCTCGCTACGTATCTGTTTGCCCGCG--GTGG + + 260 270 280 290 300 + 1 HINW.004 ::::::::::::*:D::::::::::::::::::: + 7 HINW.018 ::::::::::::::::::::CA:::::T:*:::*::::::::::::CA: + -4 HINW.017 ..............................................A... + 3 HINW.009 :::::::::::::::V::::::::::::::::::::::::::::*AV::: + -6 HINW.028 ......................A... + AATTACAGCGTTCCCTATTGACGGGCGCATCCACGCTGATTCTCTT-CTG + +.end lit +.left margin1 +@32. TX 3 @Extract gel readings +.left margin2 +.para +Used to make copies of the aligned gel readings in a database, +to write them into separate files, and to write a + +corresponding file of file names. It operates in two modes: either all gel + +readings are extracted, or only those at the ends of contigs. + +.para +Choose which mode of operation is required and supply a file of file + +names. +.para +The gel readings are given their original + +names. +If used to extract the gel readings from the ends of contigs the function + is +useful for checking for missed contig joins: the file of file names can be + +used with the auto assemble function to recompare these gel readings, + +and each should only overlap one contig. Any that overlap two contigs + +will identify possible joins. +.para +If the option is used to extract all the gel readings from a database, a + +subsequent run of "auto assemble" can reconstitute a database which has + +been corrupted. This rarely occurs and is usually necessitated by a + +user employing "alter relationships" incorrectly without first having + +made a copy. +.left margin1 +@1. TX 0 @Help +.left margin2 +.PARA +Help is available on the following topics : + +.LEFT MARGIN1 +@2. TX 0 @Quit +.LEFT MARGIN2 +.PARA +This command stops the program and is the only safe way to terminate a + +run +of the program that has altered the contents of the database in any way. + +.left margin1 +@3. TX 1 @Open a database +.LEFT MARGIN2 +.PARA +Opens existing databases or allows new ones to be started. The function + is +automatically called into operation +when the program is started but can also be selected + +from the general menu. +.para +Choose to open an existing database or start a new one, or if ! is typed +when the program is first started, enter the program without opening a +database. Supply a project + +database name, and if it already exists, the "version". If starting a new + +database define the database size and if it is for DNA or protein sequences. +The database size is an initial size for the database. It can be increased +later during the project. It is the sum of the number of gel +readings plus the number of contigs. +.para +Database names can have from one to 12 letters and must not include full + +stop (.). The database is made from five separate files. If the database + is +called FRED then version 0 of database FRED comprises files FRED.AR0, + +FRED.RL0, FRED.SQ0, FRED.TG0 and FRED.CC0. The version is the last symbol in the file names. + +Only this program + can read these files. If the "copy database" option is used it + +will ask the user to define a new "version". +.para +For normal use the maximum gel reading length is set to 512 characters, + +but when a database is started the user may choose lengths of either + + 512, +1024, 1536..., 4096. Normally the program is used to handle DNA + +sequences but many of the functions also work on protein sequences. The + +choice of sequence type is made when the database is started. + +.para +The contigs are not stored on the disk as the user sees them displayed on + +the screen. Each gel reading is stored with sufficient information about + +how it overlaps other gel readings so that the program can work out how + +to +present them aligned on the screen. We refer to this extra data as "the +relationships" and it is explained below. + +The database comprises 5 separate files. + +.left margin2 + 1. a working version of each gel reading. This is the version of + the gel reading +that is in the database and initially it is an exact copy of + the original sequence (known as the archive) + but it is edited and manipulated to align it + with other gel readings. + +.left margin2 + 2. the file of relationships. This file contains all of the + + information that is required to assemble the working versions +into + + contigs during processing; any manipulations on the data use this + + file and it is automatically updated at any time that the + + relationships are changed. The information in this file is as + + follows: +.left margin2 + (A) Facts about each gel reading and its relationship to +others +("gel + + descriptor lines"): + +.left margin2 + (a) the number of the gel +reading (each gel reading is given a number as it is + + entered into the database) + +.left margin2 + (b) the length of the sequence from this gel reading + +.left margin2 + (c) the position of the left end of this gel +reading relative to the left + + end of the contig of which it is a member + +.left margin2 + (d) the number of the next gel +reading to the left of this gel reading + +.left margin2 + (e) the number of the next gel reading to the right + +.left margin2 + (f) the relative strandedness of this gel +reading , ie whether it is in + + the same sense or the complementary sense as its archive. + +.left margin2 + (B) Facts about each contig ("contig descriptor lines"): + +.left margin2 + (a) the length of this contig + +.left margin2 + (b) the number of the leftmost gel +reading of this contig + +.left margin2 + (c) the number of the rightmost gel reading of this contig. + +.left margin2 + (C) General facts: + +.left margin2 + (a) the number of gel readings in the database + +.left margin2 + (b) the number of contigs in the database. + +.left margin2 + 3. the file of archive names. This is simply a list of the names + + of each of the archive files in the database but on line number + + 1000 we also store the size of the database. ie the number of lines + + of information allowed in the database files. This file always has + + 1000 lines but the length of the file of relationships and the file + + of working versions can be set by the user when creating a +database + + or when copying from one to another. +.left margin2 + 4. the file of tags (annotation). +This consists of linked lists of tag information for each sequences in the +database. +Tags are created by the user as annotation, or by xdap as records of edits or +for storing cutoff information. +As the number of tags can grow without limit, so can this file. +For each gel there is a header record, which contains the record number of +the start of the linked list for that gel. On line IDBSIZ there is a record +containing information about the file such as its present length and if there +are any free "tag" slots to be reused in the file. + + 5. the file of comments (annotation). +This consists of linked lists of comment fragments. +Comments are created by the user as a message attached to annotation, +or by the system to store cutoff information. +Comments are character strings of any length. +Comments longer than 40 characters are broken up into fragments, each 40 +characters long, and are chained together in a link list. +As the number of comments can grow without limit, so can this file. + +.para + Structure of the database files +.para + 1. The file of relationships +.para + The file contains IDBSIZ lines of data: + the general data are stored on line IDBSIZ; data about gel +readings are + stored from line 1 downwards; data about contigs are stored from + line IDBSIZ-1 upwards. A database of 500 lines containing 25 gel +readings and 4 contigs would have a file + of relationships as is shown below. +.lit + + + --------------------------------------------- + 1 Gel descriptor record + 2 " " " + 3 " " " + 4 " " " + 5 " " " + ' ' ' ' + ' ' ' ' + 25 " " " + 26 Empty record + ' ' ' + + ' ' ' + 495 ' ' + 496 Contig descriptor record + 497 " " " + 498 " " " + 499 " " " + 500 Number of gel readings=25, Number of contigs=4 + --------------------------------------------- + + The arrangement of the data in the file of relationships + +.end lit +As each new gel reading is added into the database a new line is added + to the end of the list of gel descriptor + lines. If this new gel reading does not + overlap with any gel readings + already in the database a new contig line is + added to the top of the list of contig lines. If it overlaps with + one contig then no new contig line need be added but if it overlaps + with two contigs then these two contigs must be joined and the + number of contig lines will be reduced by one. Then the list of +contig + lines is compressed to leave the empty line at the top of the list. + Initially the two types of line will move towards one another but + eventually, as contigs are joined, the contig descriptor lines will + move in the same direction as the gel descriptor + lines. At the end of a + project there should be only one contig line. The database is thus + capable of handling a project of 998 gels. +.para + 2. Structure of the working versions file +.para + The working versions of gel readings are stored in a file of + IDBSIZ lines each containing 512 characters. Gel reading +number 1 is stored on line + 1, gel reading number 2 on line 2 and so on. +.para + 3. Structure of the archive names file +.para + This file, unlike the others, always has 1000 lines each 10 + characters in length. Its length is fixed because line 1000 is used + to store IDBSIZ the database size and the programs need a definite + location from which to read this number. +.para + 4. Structure of the tag file +.para +This file initially starts with IDBSIZ lines, and is expanded as new tags are +created. +Information about the length of the file, and which tag records are reusable +is stored on line IDBSIZ. +A database of 500 lines would have a file of tags as shown below. +.lit + + --------------------------------------------- + 1 Tag descriptor record + 2 " " " + 3 " " " + 4 " " " + 5 " " " + ' ' ' ' + ' ' ' ' + 497 " " " + 498 " " " + 499 " " " + 500 Length of file=N, Free list=0 + 501 Tag record + 502 " " + 503 " " + ' ' ' + ' ' ' + N-2 " " + N-1 " " + N Tag record + --------------------------------------------- + + The arrangement of the data in the file of relationships + +.end lit +As each new tag is added to the database, a check is made in the +file descriptor record at line IDBSIZ. If the list of reusable records is 0, +the file is extended by one line. Otherwise the new tag is assigned to +record at the head of the freelist. +When tags are deleted, they are added to the free list in the file descriptor +record. +.para + 5. Structure of the comment file +.para +This file initially starts with 1 line, and is expanded as new annotation is +created. +Information about the length of the file, and which comment records are reusable +is stored on the first line. +.lit + + --------------------------------------------- + 1 Length of file=N, Free list=0 + 2 Comment fragment + 3 " " + 4 " " + ' ' ' + ' ' ' + N-2 " " + N-1 " " + N Comment fragment + --------------------------------------------- + + The arrangement of the data in the file of relationships + +.end lit +As each new comment is added to the database, a check is made in the file +descriptor record at line 1. If the list of reusable records is 0, +the file is extended to hold the new comment. Otherwise the new comments is +assigned to records starting with the head of the freelist. +When comments are deleted, the discarded records are added to the free list in +the file descriptor record. +.para + There are various checks within the programs to + protect users from themselves:- +.left margin2 + 1. All user input is checked for errors - e.g. reference to + non-existent gel +readings or contigs, incorrect positions in the + contig or gel readings. +.left margin2 + 2. Before entering a gel reading the system checks to see if a + file of the same name has already been entered. +.left margin2 + 3. Join will not allow the circularising of a contig. +.left margin2 + 4. Both enter and join functions restrict the region + that the user is allowed to edit (using edit contig) to the + region of overlap. +.left margin2 +5. Users may escape from any point in the program. +.left margin2 +6. Help is available from all points in the program. +.SK2 +.LEFT MARGIN2 +IT IS ESSENTIAL THAT USERS DO NOT KILL THE PROGRAM WHILE IT IS +DOING +ANYTHING THAT INVOLVES CHANGING THE CONTENTS OF THE +DATABASE. I.E DURING AUTO ASSEMBLE, +COMPLETE ENTRY, COMPLETE JOIN, COMPLEMENT CONTIG, EDIT CONTIG, AND SCREEN +EDIT. +This could +corrupt the database so badly that it is impossible to fix. The program +should always be left using the QUIT option. + +.left margin1 +@4. TX 3 @Edit contig +.LEFT MARGIN2 +.PARA +The Contig Editor is a mouse-driven editor that can insert, +delete and change gel reading sequences. +.para +The Contig Editor allows scrolling from one end of a contig to the other +using the scroll bar and scroll buttons. Action of mouse button presses +when the mouse pointer is in the scroll bar: +.sk1 +.lit + Middle Mouse Button Set editor position + Left Mouse Button Scroll forward one screenful + Right Mouse Button Scroll backwards one screenful +.end lit +.sk1 +The four scroll buttons operate as follows: +.sk1 +.lit + "<<" Scroll left half a screenful + "<" Scroll left one character + ">" Scroll right one character + ">>" Scroll right half a screenful +.end lit +.para +The Editor cursor can be positioned anywhere in the edit window by +moving the mouse pointer over the character of interest, then pressing the +left mouse button. The Editor cursor can also be moved by using the +direction arrow keys. +.para +The editor operates in two main edit modes - Replace and Insert. Replace allows +a character to be replaced by another. Insert allows characters to be +inserted into a gel reading sequence. Characters are entered by typing +them from the keyboard. Only valid characters are permitted. +Characters can be deleted by positioning the cursor one character to the right, +then pressing the delete key. +Normally Insert and Delete apply to the consensus line of the contig ONLY. +This restraint can be overridden by using the "Super Edit" mode of +operation, THOUGH IT IS NOT RECOMMENDED. +.para +Edits can also be performed on the consensus, though they are +restricted to insertion and deletion of padding characters ("*"). +These edits also have special meanings. +A deletion will delete ALL characters at the position to the left +of the cursor in the contig, and move the relative positions of all +sequences starting to the right of the cursor position left one +character. +An insertion will insert the character typed ("*") into ALL gel +reading sequences at the cursors position in the contig, and move the +relative positions of all sequences starting to the right of the cursor +position right one character. +.para +The effect of the last edit can be undone by pressing the "Undo" button +at the top of the editor window. +.para +The cursor will automatically be positioned at the next problem when the +"Find Next Problem" button is selected. The next problem is where the +consensus shows either an ambiguity ("-") or a pad ("*") character. +.para +The edits to the contig can be saved by pressing the "Leave Editor" +button and replying "Yes" to the prompt to "Save changes?". As no changes +are made to the working copy of your database til this point it +is possible to abort the editor if +the edit session ends up in an unsatisfactory state (ie if you've +stuffed it up!) +.left margin1 +.sk3 +Displaying Traces +.left margin2 +.para +The original data from which the gel reading sequences where derived can +be seen by double clicking (two quick clicks) with the middle mouse button +on the area of interest. The trace will be displayed with the point +clicked at the centre of the trace viewport. +.para +All traces that are displayed are maintained in one window, called the Trace +Manager. The Trace Manager will only display four traces maximum. When four +traces are already being managed and a new one is requested, the one at the top +of the Trace Manager is removed and the new one is added to the bottom. +Traces can be removed individually by using the "quit" button in the panel next +to the trace. +.left margin1 +.sk3 +Extending Reads Using Cutoff Information +.left margin2 +.para +Sequence data read in from Automated Fluorescent sequencing machines +trace files processed through the program ted +will have the discarded sequence (vector at start and poor read at +end) available to the contig editor. To display the cutoff +information, press the "Display Cutoff" button at the top of the +editor window. +The cutoff sequence appears in grey. This sequence can be incorporated +into the editable sequence, by moving the cutoff position. This is +done by positioning the cursor at the end of the gel sequence, and +using Meta-Left-Arrow and Meta-Right-Arrow to adjust the point of cutoff. +The Meta key is a diamond on the Sun keyboard. +.left margin1 +.sk3 +Pop-up menu +.left margin2 +.para +A pop-up menu is revealed by depressing the "Control" key on the keyboard +and at the same time pressing the left mouse button. The menu has the following +functions: +.lit + + Search + Save Contig + Create Tag + Edit Tag + Delete Tag + +.end lit +"Save Contig" is described above. +Searching and operations on tags are described below. +.left margin1 +.sk3 +Searching +.left margin2 +.para +Selecting "Search" brings up a +window which can remain present during normal editor operation. The +window allows the user to select the direction of search, the type of +search and a value to search on. The value is entered into the value +text window. Then pressing the "search" button +performs the search. If successful, the cursor is positioned and +centred accordingly. An audible tone indicates failure. Pressing the +"ok" button removes the search window. The search window is +automatically removed when the contig editor is exited. +.sk1 +There are seven different search modes: +.sk1 +1. Search by position +.sk1 +This positions the cursor at the numeric position specified in the +value text window. Eg a value of "1234" causes the cursor to be placed +at base number 1234 in the contig. Positioning withing a gel reading is +achieved by prefixing the number with the "@" character, eg "@123" +positions the cursor at base 123 of the sequence in which the cursor +lies. Relative positions can be specified by prefixing the number with +a plus or minus character. Eg "+1234" will advance the cursor 1234 +bases. If possible, the cursor is positioned within the same sequence. +The direction buttons have no effect on the operation of "search +by position". +.sk1 +2. Search by reading name +.sk1 +This positions the cursor at the left end of the gel reading specified +in the value text window. If the value is prefixed with a slash is is +assumed to be a gel reading name. Otherwise it is assumed to be a gel +reading number. Eg "123" positions the cursor at the left end of gel +reading number 123. "/a16a12.s1" positions at the start of reading +a16a12.s1. If the value was "/a16" the cursor is positioned at the +first reading which starts with "a16". The direction buttons have no +effect on the operation of "search by position". +.sk1 +3. Search by tag type. +.sk1 +This positions the cursor at the start of the next tag which has the +the same type as specified by the type value menu. To change the type, +select off the menu that pops up when the mouse is clicked on the +button labeled "Type:". The search can be performed either forwards +or backwards of the current cursor position. To find all tags, use +"search by annotation", with a null text value string. +.sk1 +4. Search by annotation. +.sk1 +This positions the cursor at the start of the next tag which has a +comment containing the string specified in the value text window. The +search performed is a regular expression search, and certain +characters have special meaning. Be careful when your value string +contains ".", "*", "[", "^" or "$". The search can be performed either +forwards or backwards from the current cursor position. +.sk1 +5. Search by sequence. +.sk1 +This positions the cursor at the start of the next piece of sequence +that matches the value specified in the text value window. The search +is for an exact match, which means the case of value string is +important. The search is performed on the gel readings themselves, +rather than the consensus sequence. The search can be performed either +forwards or backwards from the current cursor position. +.sk1 +6. Search by problem. +.sk1 +This positions the cursor at the next place in the consensus sequence +which is not an "A", "C", "G" or "T". The search can be performed +either forwards or backwards from the current cursor position. +.sk1 +7. Search by quality +.sk1 +This positions the cursor at the next place in the consensus sequence +where the consensus calculation for each strand disagrees. When only +sequences on one strand is present, the search will stop at every +base. The search can be performed either forwards or backwards from the +current cursor position. +.left margin1 +.sk3 +Annotation +.left margin2 +.para +Parts of a sequence can be annotated, to record the positions of primers used +for walking, or to mark sites, such as compressions that have caused problems +during sequencing. +The consensus sequence CANNOT be annotated. +.para +To annotate a piece of sequence first select the part of sequence +using the mouse buttons. Use the left mouse button to position the start of the +selection, and while this button is being held down, move the mouse to extend. +The selection can be extended further using the right mouse button. +.para +To create annotation, invoke the pop-up menu, and select the "Create Tag" +function. A small "tag editor" will appear which +allows you to select the type of the +annotation from a pull-down menu, and specify a comment if desired. +To select a new type pull down the Type menu, and select the entry desired. +To enter a comment, simply type into the text window in the tag editor. +The annotation is created when the "Leave" button on the tag editor, +and is displayed in the colour defined in the tag database file (TAGDB). +.para +To edit existing annotation, +position the cursor with the left mouse button +on the tag, and select the +"Edit Tag" +off the pop-up menu. +This invokes the tag editor, and changes to the type and comment of the +annotation can be made. The tag is updated when the "Leave" button is pressed. +.para +To delete an existing annotation, +position the cursor with the left mouse button +on the tag, and select the +"Delete Tag" +off the pop-up menu. +.left margin1 +.sk3 +NOTE: +.left margin2 +.para +As the Contig Editor is a very powerful tool, it is possible that the alignment +of the gel reading sequences has unexpectedly been disrupted. +This can easily happen to parts of the contig that lie to the right +of the screen if excessive use has been made of the "Super Edit" facility. +Until familiar with "Super Edit" it would benefit the sequencer to quickly +scan through the contig after editing to check that bad alignments have not +been created. +.left margin1 +@9. T 3 @Screen edit +.LEFT MARGIN2 +.para +THIS OPTION IS NO LONGER AVAILABLE IN XDAP. USE EDIT CONTIG +.para +Gives access to the system editor on the machine (for example EDT on a VAX) +and allows users to edit contigs. The contigs are presented as for +"display contig" and the program will +reconstitute the contig's sequences and relationships when the editor is +exited. +.para +To screen edit a contig set the line length to 50 characters, +select the contig to edit, and supply the name of a temporary file in which +the editing will be performed. +After a short pause the system +editor will present the first page of the file. Edit the file obeying the +rules given below. Exit from the editor and affirm the intention of +returning the contig to the database. The program will put the contig +back into the database. +.para +Rules for screen editing +.para +There are some limitations on the changes that can be made to the contigs +when using the screen editor. Users are unlikely to want to break the +rules +in order to achieve changes to contigs, but nevertheless the +constraints need to be defined and they are given below. +.para +Alignments must be maintained during editing. +Whole lines of sequence should not be deleted or added unless the +order +of the gel readings in the contig is preserved. +Each line in the +contig display consists of gel reading numbers, their names and 50 +character sections of sequence. Insertions are limited in the following +way. +No line of sequence can be extended rightwards more than 10 characters +beyond the end of a full length line (a full length line is 50 characters +long). Only one character can be added to the left end of full length +lines, but sections of sequence beginning further into a line + can be extended leftwards up to an equivalent position. Do not delete any +non-sequence lines in the file. +.para +Before returning the contig to the database the program checks that the +rules have been obeyed. If an error is found the number of the erroneous +line in the +file is displayed and the contig will not be changed. +.left margin1 +@5. TX 1 @Display a contig +.LEFT MARGIN2 +.para +Used to show the aligned gel readings for any part of a contig. The + +number, name and strandedness of each gel reading is shown and the + +consensus is written below. +.para +If required identify the contig, and then the start and end points of the + +region to display. +.para +The display can be directed to a disk file using "direct output to disk". + +These files are required by options: "screen edit" and "highlight + +disagreements", and printed copies of them +are very useful for marking corrections prior to + +using the editors. +.para + Below is an example showing the left end of a contig from + position 1 to 200. Overlapping this region are gels 6,3,5,17and 12; +6, 3 and 5 +are in reverse orientation to their archives (denoted by a minus sign) + There are a few uncertainty codes and a few padding + characters in the working versions, but the consensus (shown +below + each page width) has a definite assignment for almost every +position. +.lit + + 10 20 30 40 50 + -6 HINW.010 GCGACGGTCTCGGCACAAAGCCGCTGCGGCGCACCTACCCTTCTCTTATA + CONSENSUS GCGACGGTCTCGGCACAAAGCCGCTGCGGCGCACCTACCCTTCTCTTATA + + 60 70 80 90 100 + -6 HINW.010 CACAAGCGAGCGAGTGGGGCACGGTGACGTGGTCACGCCGCGGACACGTC + -3 HINW.007 GGCACA*GTC + CONSENSUS CACAAGCGAGCGAGTGGGGCACGGTGACGTGGTCACGCCG-G-ACA-GTC + + 110 120 130 140 150 + -6 HINW.010 GATTAGGAGACGAACTGGGGCG3CGCC*GCTGCTGTGGCAGCGACCGTCG + -3 HINW.007 GATTAG4AGACGAACTGGGGCGACGCCCG*TGCTGTGGCAGCGACCGTCG + -5 HINW.009 GGCAGCGACCGTCG + 17 HINW.999 AGCGACCGTCG + CONSENSUS GATTAGGAGACGAACTGGGGCGACGCC-G-TGCTGTGGCAGCGACCGTCG + + 160 170 180 190 200 + -6 HINW.010 TCT*GAGCAGTGTGGGCGCTG*CCGGGCTCGGAGGGCATGAAGTAGAGC* + -3 HINW.007 TCT*GAGCAGTGTGGGCGCTGC*CGGGCTCGGAGGGCATGAAGTAGAGC* + -5 HINW.009 TCT*GAGCAGTGTGGGCG*T*G*CGGGCTCGGAGGGCATGAAGTAGAGC* + 17 HINW.999 TCTCGAGCAGTGTGGGCGCTG**CGGGCTCGGAGGGCATGAAGTAGAGCG + 12 HINW.017 GTAGAGC* + CONSENSUS TCT*GAGCAGTGTGGGCGCTG-*CGGGCTCGGAGGGCATGAAGTAGAGC* +.END LIT +.left margin1 +@6. TX 1 @List a text file +.LEFT MARGIN2 +.PARA +This option allows users to list text files on the screen. It can be used +to read a file containing notes, for checking files written to disk etc. The +user is asked to type the name of the file to list. +.left margin1 +@8. TX 1 @Calculate a consensus +.LEFT MARGIN2 +.para + Calculates a consensus sequence either for the whole database or + +for selected contigs. The consensus is written to a file named by the + user. +.left margin2 +Supply a file name, choose between whole database or selected contigs. +.para + Symbols for uncertainty in gel readings +.para +In order to record uncertainties when reading gels the codes shown + +below can be used. Use of these codes permits us to extract the + +maximum amount of data from each gel and yet record any doubts by + +choice of code. The program can deal with all of these codes and any + +other characters in a sequence are treated as dash (-) characters. + + +.lit + + SYMBOL MEANING + + 1 PROBABLY C + 2 " T + 3 " A + 4 " G + D " C POSSIBLY CC + V " T " TT + B " A " AA + H " G " GG + K " C " C- + L " T " T- + M " A " A- + N " G " G- + R A OR G + Y C OR T + 5 A OR C + 6 G OR T + 7 A OR T + 8 G OR C + - A OR G OR C OR T + a A set by auto edit + c C set by auto edit + g G set by auto edit + t T set by auto edit + * padding character placed by auto assembler + else = - + +.end lit + +.LEFT MARGIN2 + The DNA consensus algorithm +.para +The "calculate consensus" function, the "display contig" routine and the + +"show quality" option use the rules outlined here to calculate a + +consensus from aligned gel readings. Note that "display contig" +calculates +a consensus for each page width it displays (it does not use the + +consensus sequence file calculated by the consensus function). + +.LEFT MARGIN2 +.para +We have 6 possible symbols in the consensus sequence: A,C,G,T,* and -. The +last symbols is assigned if none of the others makes up a sufficient +proportion of the aligned characters at any position in the contig. The +following calculation is used to decide which symbol to place in the +consensus at each position. +.para +Each uncertainty code contributes a score +to one of A,C,G,T,* and also to the total at each point. Symbols like R +and Y which don't correspond to a single base type contribute only to the +total at each point. The scores are shown below. +.lit + definite assignments ie A,C,G,T,B,D,H,V,K,L,M,N,a,c,g,t,* =1 + + probable assignments ie 1,2,3,4 = 0.75 + + other uncertainty codes including R,Y,5,6,7,8,- = 0.1 +.end lit +.para +A cutoff score of 51% to 100% is supplied by the user. (When the program +starts this is set to 75%. See "set display parameters"). +At each position in the contig we calculate the total score for each of +the 5 symbols +A,C,G,T and * (denote these by Xi, where i=A,C,G,T or *), +and also the sum of these totals +(denote this by S). Then if 100 Xi / S > the cutoff for any i, symbol i is +placed in the consensus; otherwise - is assigned. +.para +Notice that S does not equal the number of times the sequence has been +determined, but is the score total, and hence we are less likely to put a - +in the consensus. For the "examine quality" algorithm each strand is +treated separately but the calculation is the same. (It was originally +different). +.para +Format of the consensus sequence ( and vector sequences). +.para +A consensus sequence file may contain the consensus for several contigs + +and so we identify each of them by preceding them by a 20 character + +title. The title is of the form <---LAMBDA.076-----> ( where LAMBDA is + +the project name and gel reading number + + + 76 is the leftmost gel +reading to contribute to this consensus sequence). + + + The angle brackets <> and the three digit number precede by a . + +are important to some processing programs. +.left margin1 +@25. TX 1 @Show relationships +.LEFT MARGIN2 +.para + Used to show the relationships of the gel readings in the database in + +three ways - +.LEFT MARGIN2 + (a) All contig descriptor lines followed by all gel descriptor + lines. +.LEFT MARGIN2 + (b) All contigs one after the other sorted, i.e. for each + contig show its contig descriptor line followed by all its + gel descriptor lines sorted on position from left to right +.LEFT MARGIN2 + (c) Selected contigs: show the contig line and, in order, + those gel readings that cover a user-defined region. + Note that this output can be directed to a disk file by + prior selection of "disk output". +.LEFT MARGIN2 +.para + Below is an example showing a contig from position + 1 to 689. The left gel reading is number 6 and has archive +name HINW.010, the +rightmost gel reading is number 2 and is has archive name HINW.004. +On each gel descriptor line is shown: + the name of the archive version, the gel number, the position of the + left end of the gel reading relative to the left end of the contig, the + length of the gel +reading (if this is negative it means that the gel reading is in + the opposite orientation to its archive), the number of the gel +reading to + the left and the number of the gel reading to the right. +.lit + + + CONTIG LINES + CONTIG LINE LENGTH ENDS + LEFT RIGHT + 48 689 6 2 + GEL LINES + NAME NUMBER POSITION LENGTH NEIGHBOURS + LEFT RIGHT + HINW.010 6 1 -279 0 3 + HINW.007 3 91 -265 6 5 + HINW.009 5 137 -299 3 17 + HINW.999 17 140 273 5 12 + HINW.017 12 193 265 17 18 + HINW.031 18 385 -245 12 2 + HINW.004 2 401 -289 18 0 + +.end lit +.left margin1 +@21. TX 3 @Enter new gel reading +.LEFT MARGIN2 +.para +THIS OPTION IS NO LONGER AVAILABLE IN XDAP. USE AUTO ASSEMBLE +.para +Used to enter new gel readings into the +database. The new gel reading must have previously been compared with +the +contents of the database by use of " auto assemble" in order to ascertain +if it overlaps any previously entered data. +.para +The user is expected to know: if +the gel reading overlaps; if so which contig it overlaps; if so where it +overlaps. The program takes the user through a series of question to +establish the nature of the overlap and then displays the overlap. The +user +is then offered a number of options, including editors for the new gel +reading and the contig, to enable the correct alignment of the gel reading +throughout its whole length. +.left margin2 + +Supply the name of the gel reading file. +If the gel +reading has been entered before the program will not permit + +entry. +The program gives the gel reading a unique number and asks if the + +sequence overlaps any data already in the database (reported by "auto + +assemble"). + +If it does not, entry is complete. +If it does overlap the + +dialogue +continues with the program asking if the gel readings overlaps "in the + +normal sense", if not it will automatically complement the sequence. + +Then supply the number of the contig the gel reading overlaps (as + +reported by "auto assemble"). +.para +Overlaps are divided into two types: those for which the new gel reading + +protrudes from the left end of the contig it overlaps, and those for which + +it does not. The program asks about this with the question "Left end of +gel +reading is inside contig". If this is true the program will go on to ask for + +the position in the contig of the left end of the new gel reading. If it is + not +true the program will ask for the position in the new gel reading of the + +left end of the contig. +.para +Once this is completed the program will display the first 50 bases of + +the overlap. +The gel readings in the contig and their consensus are displayed with the + +new gel reading underneath. The mismatches are shown by *'s on the +next +line down. +For example: +.lit + + + 60 70 80 90 100 + -6 HINW.010 CACAAGCGAGCGAGTGGGGCACGGTGACGTGGTCACGCCGCGGACACGTC + -3 HINW.007 GGCACA*GTC + CONSENSUS CACAAGCGAGCGAGTGGGGCACGGTGACGTGGTCACGCCG-G-ACACGTC + NEWGEL CACAAGCGAGCGAGAGGGGCACCGTGACGTGGTCACGCCGGGGACACGTC + MISMATCH * * * + 10 20 30 40 50 + +.end lit +.para +The program then needs to know if the position of the left end of the +overlap is correct. + +If it is the user should type return, if not, 1 and the program will ask for +the +new position and display it. + +.LEFT MARGIN2 +The program now offers a number of options to allow the + user to align the new gel reading +correctly over its whole length with + the data already in the contig. It is important that + sufficient edits are made to the new gel reading +or the sequences in the + contig at this stage to get the alignment correct, because once + entry is completed, the alignment is fixed and cannot easily be + changed (see "alter relationships"). + Alignment can be achieved +by making + insertions or deletions but deletion of data requires the + original gels to be checked. For this reason at entry we + usually make only insertions to achieve alignment. We use X or + asterisks (*) as padding characters to achieve alignment and + so can, if required, + distinguish padding characters from characters assigned from + reading gels. +.LEFT MARGIN2 +.para +The options available are: +.lit + ? = HELP + ! = Give up + 3 = Complete entry + 4 = Edit contig + 5 = Display overlap + 6 = Edit new gel reading + +.end lit + +.sk1 +.para +1. HELP gives this information. +.para +2. Give up allows users to change their minds about entering the new gel +reading. The program will ask the user to +confirm this choice. +.para +3. Complete entry is the command to add the new gel reading to the +contig. The +program updates the relationships accordingly. The user is asked to +confirm +this command. +.para +4. Edit contig gives the user access to a simple editor that allows +insertions, deletions and changes to be made to the contig. The editor +maintains alignments by making the same number of insertions or +deletions +in all sequences covering the edit position. +The program + protects the user by allowing edits only within + the region of overlap. +.para +5. Display allows display of the region of overlap only. This + is defined by the relative positions in the contig. The + default is the whole of the region of overlap. +.para +6. Edit new gel reading allows the new gel reading to be edited using a +simple editor. +.left margin1 +@23. TX 3 @ Complement a contig +.LEFT MARGIN2 +.PARA + This function will complement and reverse all of the gel +readings in a + contig. It automatically reverses and complements each gel + reading sequence, reorders left and right neighbours, recalculates +relative + positions and changes each strandedness. +.PARA + The only user input required is to identify the contig to + complement by the number or name of a gel reading it contains. +DO NOT KILL THE +PROGRAM DURING THIS STEP! +.left margin1 +@22. TX 3 @ Join contigs +.LEFT MARGIN2 +.PARA +This function joins contigs interactively using a mouse driven editor. +The operation of this editor is very similar to the Contig Editor +described in "@4 Edit". + +.para +It allows the +user to align the ends of the two contigs by editing each +contig separately. It is important that the alignment achieved is +correct because once the join is completed the alignment is fixed. +The program needs to know which two contigs to join. +.para +First specify which two contigs are to be joined. +The user should identify the two +contigs. First the left contig and then the right. +The program checks that the two contig numbers are different (it will not +allow circles to be formed!) +.para +The Join Editor consists of two Contig Editors in between which is sandwiched +a disagreement box. This disagreement box shows exclamation marks to +denote mismatches between the two consensuses. +.para +For example, the display will look something like this: +.lit + + 1460 1470 1480 1490 1500 + 56 HINW.100 TCT*GAGCAGTGTGGGCGCTG*CCGG + 33 HINW.300 TCT*GAGCAGTGTGGGCGCTGC*CGGGCTCGGAGGG + -25 HINW.090 TCT*GAGCAGTGTGGGCG*T*G*CGGGCTCGGAGGG + 19 HINW.123 TCTCGAGCAGTGTGGGCGCTG**CGGGCTCGGAGGGCATGAAGTAGAGCG + CONSENSUS TCTCGAGCAGTGTGGGCGCTG-CCGGGCTCGGAGGGCATGAAGTAGAGCG + MISMATCH ! !!!!!! + 10 20 30 40 50 + -6 HINW.010 TCTCGAGCAGTGTGGGCGCTGCCCGGGCTCGGAGGGCATGAAGTTAGAGC + -3 HINW.007 TGGGCGCTGCCCGGGCTCGGAGGGCATGAAGT*AGAGC + -5 HINW.009 GCTCGGAGGGCATGAAGT*AGAGC + CONSENSUS TCTCGAGCAGTGTGGGCGCTGCCCGGGCTCGGAGGGCATGAAGTTAGAGC + +.END LIT +.para +.para +The best strategy for joining is to +identify the exact position of overlap. This is defined as +the position in the left contig that the leftmost character of the right +contig overlaps. +The overlap must be of at least one character. +Use the scroll bar and the scroll buttons (`<<',`<',`>',and`>>') +for positioning the relative positions of the two contigs. +.para +The join position can be fixed in position +by pressing the `lock' button at the top of the Join Editor. +Locking allows the two contigs to be scrolled as one when using the scroll bar +and buttons, the left ends always in the same position relative to each +other. +.para +Once locked, it is best to proceed to the right along the contigs, inserting +padding characters (`*') into the consensuses to minimise the +disagreements. +.para +It is essential that the user aligns the two contigs throughout the whole +region of overlap before completing the join because it is only at this +stage that the two contigs can be edited independently. Once the join is +completed the alignment can only be altered using the routines supplied +by "alter relationships". +.para +The join can be completed by pressing the `Leave Editor' button. The +percentage mismatch is displayed, and the user is required to confirm that +they want to perform the join. +.left margin1 +@24. TX 1 @ Copy the database +.LEFT MARGIN2 +.PARA +Used to make a copy of the database. If required the database size can be + +altered using this option. The "version" of a database is encoded as the + +last letter in the names of the five files that contain the database. + +.para +Supply a "version" number (the default is version 1), and if required + +select a new size for the database. The size of a database is the number + of +lines of information it can hold. It needs a line for each gel reading and + +another for each contig. +.left margin1 +@19. TX 1 @ Check database +.LEFT MARGIN2 +.para +Used to perform a check on the logical consistency of the + database. No user intervention is required. +.para + The following relationships are checked: +.LEFT MARGIN2 + 1. If gel reading A thinks gel reading B is its left + neighbour + +does B think A is + its right neighbour? + The error message is +.left margin2 +"Hand holding problem for gel reading A" +.left margin2 +followed by the + gel descriptor lines for gel readings A and B. +.LEFT MARGIN2 + 2. Are there any contig lines with no left or right +end gel readings? + The error message is +.left margin2 +"Bad contig line number A" +.LEFT MARGIN2 + 3. Do the gel readings that are described as left ends on +contig + lines agree that they are left ends? + The error message is +.left margin2 +"The end gel readings of contig A have outward neighbours" +.LEFT MARGIN2 + 4. Are there gel readings that are in more than one contig? + The error message is +.left margin2 +" Gel number A is used N times" +.LEFT MARGIN2 + 5. Are there gel readings that are not in any contig? + The error message is +.left margin2 +" Gel number A is not used" +.LEFT MARGIN2 + 6. Do the relative positions of gel readings agree with +their + position as defined by left and right neighbourliness? + The error message is +.left margin2 +" Gel number A with position X is left neighbour of gel number B with +position Y" +.LEFT MARGIN2 + 7. Are there any loops in contigs? If so no further + checking is done. + The error message is +.left margin2 +" Loop in contig n no further checking done, but gel reading numbers follow" +.left margin2 + The + program then prints the gel reading numbers in the looped +contig up +to + the start of the loop. +.LEFT MARGIN2 +8. Are there any contigs of length <1? The error message is +.left margin2 +" The contig on line +number x has zero length" +.LEFT MARGIN2 +9. Are there any gel readings (used in only one contig) that have zero + +length? The error +message is +.left margin2 +" Gel number N has zero length" +.left margin2 +Note that "auto assemble" also uses this logical consistency check and + will +only tolerate a "Gel number N + is not used" error. Any other error will cause it to + +give up. + +.left margin1 +@29. TX 1 @ Examine quality +.LEFT MARGIN2 +.para +Analyses the quality of the data in a contig. It reports on the proportion + +of the consensus that is "well determined" and will display a sequence of + +symbols that indicate the quality of the consensus at each position. + +.para +Identify the contig to analyse, and the section of interest. The current + +consensus calculation cutoff score will be used to decide if each position +is +"well determined". In general the quality of a reading deteriorates along +the length of the gel and so it is also possible to use a length cutoff for +the quality calculation. Only the data from the first section of each reading +will be included in the quality calcualtion. The length is altered under +"set parameters" and is initially set to the maximum reading length. +A summary showing the percentage of the consensus +that falls into each category of quality is shown. Choose whether or not to +have the quality codes for each position of the consensus displayed. +They can be displayed as either graphics or text. +.para +The quality of the data depends on the number of times it has been + +sequenced and the particular uncertainty codes used in each gel + +reading. This function divides the data into five categories, assigning + +each +a symbol or code: +.LEFT MARGIN2 + 1. Well determined on both strands and they agree. code=0 +.LEFT MARGIN2 + 2. Well determined on the plus strand only. code=1 +.LEFT MARGIN2 + 3. Well determined on the minus strand only. code=2 +.LEFT MARGIN2 + 4. Not well determined on either strand. code=3 +.LEFT MARGIN2 + 5. Well determined on both strands but they disagree. code=4 +.LEFT MARGIN2 + A position is "well determined" if it is assigned one of the symbols +A,C,G,T when the algorithm described in the section "calculate a +consensus". +The calculation is performed +separately for each strand. +.para +If the user chooses to have the data displayed graphically the following +scheme is used. A rectangular box is drawn so that the x coordinate +represents the length of the contig. The box is notionally +divided vertically into +5 possible levels which are given the y values: -2,-1,0,1,2. +The quality codes attributed to each base position are plotted as +rectangles. +Each rectangle represents a region in +which the quality codes are identical, so a single base having a different +code from its immediate neighbours will appear as a very narrow rectangle. +.lit + + Rectangle bottom and top y values + + Quality 0 rectangle from 0 to 0 + Quality 1 rectangle from 0 to 1 + Quality 2 rectangle from 0 to -1 + Quality 3 rectangle from -1 to 1 + Quality 4 rectangle from -2 to 2 +.end lit +.para +Obviously a single line at the midheight shows a perfect sequence. +.para +Typical dialogue is shown below. +.lit + + 41.47% OK on both strands and they agree(0) + 55.48% OK on plus strand only(1) + 2.08% OK on minus strand only(2) + 0.97% Bad on both strands(3) + 0.00% OK on both strands but they disagree(4) + ? (y/n) (y) Show sequence of codes + + 10 20 30 40 50 + 1111111111 1111111111 1111111111 1111111111 1111111111 + + 60 70 80 90 100 + 1111111111 1111111111 1111111111 3111111111 1111111111 + + 110 120 130 140 150 + 1111111111 1111131111 1111111111 1111111111 1111111111 + + 160 170 180 190 200 + 1111111111 1111111111 1111111111 1111111111 1111111133 + + 210 220 230 240 250 + 1311111111 1111111111 1111111110 0000000000 0000220000 + + 260 270 280 290 300 + 0000000000 0020000000 2200000202 0002000000 0000222200 + +.end lit +.left margin1 +@26. TX 3 @ Alter relationships +.LEFT MARGIN2 +.para +Used to make what are normally illegal changes to the database. That is + +the normal checks are not done and any item in the database can be +changed independently of all others. Users need to know what they are + +doing because it is very easy to make a horrible mess. Always start by + +making a copy! +.para +By using the options here users can edit individual gel readings in contigs, +move one section of a contig relative to another, break contigs, remove +contigs, remove gel readings, etc. To give flexibility most + of the commands do only one thing. This means that several commands +may +have to be executed to complete any change. At the end of this help +section +there are notes on removing gel readings from the database. +.para +The following options are offered: +.lit + + Cancel + Line change + Edit single gel reading + Delete contig + Shift + Move gel reading + Rename gel reading + Break a contig + Alter raw data parameters + +.end lit +.left margin2 +1. QUIT returns to the main options of SAP. +.left margin2 + +2. Line change +.left margin2 + allows the user to change the contents of any line in the + +file of relationships. The line is selected by number, the + + program prints the current line and prompts for the new line. + +.left margin2 +3. Edit +.left margin2 +allows the user to edit an individual gel reading + independently of any others it may be related to. The edit +positions are relative to + the contig. The effect of this editing on the length of the + gel reading is taken care of but, if it changes the length of + a contig, + or its relationship to others, this must be accounted for (if + necessary) by use of the "line change" function. + +.left margin2 +4. Delete contig +.left margin2 +is a function that deletes a contig line by moving down + all the contig lines above by one position. It prompts only + for the line to delete. It does not delete any of the gel +readings + or gel reading +lines for the deleted contig but it does reduce the + number of contigs on line IDBSIZ by 1. + +.left margin2 +5. Shift +.left margin2 + allows the user to change all the relative positions of a + set of neighbouring gel +readings by some fixed value, i.e. it will + shift related gel readings + either left or right. It can therefore + be used to change the alignment of the gel +readings in a contig + or as part of the process of breaking a contig into two parts + (see below). It prompts for the number of the first gel +reading to + shift and then for the distance to move them (Note a + negative value will move the gel readings + left and a positive value + right). It then chains rightwards (ie follows right + neighbours) and shifts each gel +reading, in turn, up to the end + of the contig. (This means that only those gel readings + from the first + to shift to the rightmost are moved). It updates the length of + the contig accordingly. + +.left margin2 +6. Move gel reading +.left margin2 + is a function to renumber a gel reading. It moves all the information + about a gel +reading on to another line. The user must specify the +number + of the gel reading +to move and the number of the line to place it. It + takes care of all the relationships. Of course gel +readings must not be + moved to lines occupied by other gel +readings! It can be used as part + of the process of removing a gel +reading from the database (see below). + +.left margin2 +7. Rename gel reading +.left margin2 + is a function that is used to rename the archive names of + gel +readings in the database; it only changes the name in the .ARN + file of the database. + +.sk1 +.LEFT MARGIN2 +8. Break contig +.LEFT MARGIN2 +.PARA +Occasionally it is necessary to break a contig into two parts and this can be +achieved using this option. The program needs only the number of a gel +reading. This is the gel reading that will become a left end after the +break. That +is, the break is made between this gel +reading and its left neighbour. A new contig +line is created so ensure that there is sufficient space in the database. +.left margin2 +Removing gel readings from contigs +.left margin2 +.PARA +Gel +readings can be removed from contigs if they are not essential for holding the +contig together (ie are not the only gel reading covering a particular region). +Suppose the gel reading to remove is gel number +b with left neighbour a and right +neighbour c. +Using "line change" change the right neighbour of a to c, and the left +neighbour of c to a. To tidy things up: suppose there are x gel +readings in the +database; then, using "move gel reading" move gel x to line b; then, using +"line change" +decrease the number of gel +readings in the database (stored in the last line) by 1. +.sk1 +.LEFT MARGIN2 +8. Alter raw data parameters +.LEFT MARGIN2 +.PARA +Allows the user to edit the individual raw data parameters, such as +the left and right cutoff lengths and the name of the machine readable trace +file. +The user must specify the gel line to modify, and provide new values for +the length of the raw sequence including cutoff lengths, the left cutoff position, the length of the original working sequence, the machine type, and the name +of the raw data file, where these values change. +.left margin1 +@27. TX 1 @ Set display parameters +.LEFT MARGIN2 +.para +Used to redefine the parameters that control the cutoff employed by the + +consensus calculation and quality examiner, the maximum length of each +reading to include in the quality calculation, the line length used by + +the display function, the text window length used by the graphics +options, and the graphics window length used by the graphics options. +.para +The default cutoff score is 75%. The default line length is 50 characters. +For protein sequences the cutoff is always 100%. +.para +The text window used by the graphics options controls the amount of +sequence listed at the crosshair position. The graphics window controls the +"zoom" function. Both these windows are defined as the number of bases that +should be shown, to both left and right of the crosshair. +.left margin1 +@30. TX 3 @ Auto edit a contig +.left margin2 +.para +This function automatically changes characters in gel readings to make + +them agree with the consensus sequence. If employed as is intended, use + +of this function is not a criminal activity but a method that saves a large + +amount of work. All characters changed by the auto editor will appear in + +the gel readings as lowercase letters. The current consensus calculation +cutoff score is used. +.para +Identify the contig and the section to edit. The program will display a + +summary of changes made. Note that it is important to understand both + +what the auto editor does and the order in which it does it. Before + +employing the auto editor users should note all the corrections that they +require, so that after it has been used the corrections can be checked. + +.para + The +general strategy employed when collecting shotgun sequence data is to let +the contigs get fairly deep, to get a printout of a contig, +check problems against the +films, note corrections on the printout, and +make the changes using an interactive editor. +In general the consensus is correct except for places where padding +characters have been used to accommodate a single gel with an extra +character, or where the consensus is dash. The important point for the +auto +editor is that +most edits simply make the +gel readings conform to the consensus, or remove columns of pads. +.para +The new editor does the following. +.para +1) calculates a consensus for the contig (or part of a contig) to be +edited, and then uses this consensus to direct the editing of the contig +in 3 stages +.para +2) stage 1: find and correct all places where, if the order of two adjacent +characters is swapped, they will both agree with the consensus (given +that +they did not match the consensus before). These corrections are termed +"transpositions" +.para +3) stage 2: find and correct all places where there is a definite consensus +but the gel reading has a different character. These corrections are +termed +"changes". +.para +4) stage 3: delete all positions in which padding is the consensus. These +corrections are termed "deletions". +.para +All changed characters are shown in lowercase letters so it will be +obvious which +characters have been assigned by the program (except for deletions). The +number of each type of correction will be displayed. + +.LEFT MARGIN1 +@10. TX 2 @Clear graphics +.LEFT MARGIN2 +.para + Clears graphics from the screen. +.left margin1 +@11. TX 2 @Clear text +.LEFT MARGIN1 +.para + Clears text from the screen. +.left margin1 +@12. TX 2 @Draw a ruler. +.LEFT MARGIN2 +.para +This option +allows the user to draw a ruler or scale along the x axis of the screen to +help identify the coordinates of points of interest. The user can define +the position of the first base to be marked (for example if the active +region is 1501 to 8000, the user might wish to mark every 1000th base +starting at either 1501 or 2000 - it depends if the user wishes to treat +the active region as an independent unit with its own numbering starting +at +its left edge, or as part of the whole sequence). The user can also define +the separation of the ticks on the scale and their height. If required the +labelling routine can be used to add numbers to the ticks. +.left margin1 +@14. TX 2 @Reposition plots +.LEFT MARGIN2 +.para +The positions of each of the plots is defined relative to a users drawing +board which has size 1-10,000 in x and 1-10,000 in y. +Plots for +each option are drawn in a window defined by x0,y0 and xlength,ylength. +Where x0,y0 is the position of the bottom left hand corner of the window, + and xlength is the width of the window and ylength the +height of the window. +.lit + --------------------------------------------------------- 10,000 + 1 1 + 1 -------------------------------------- ^ 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 1 1 ylength 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 -------------------------------------- v 1 + 1 x0,y0^ 1 + 1 <---------------xlength--------------> 1 + --------------------------------------------------------- 1 + 1 10,000 + +.end lit +All values are in drawing board units (i.e. 1-10,000, 1-10,000). +The default window positions are read from a file "ANALMARG" when the +program is started. Users can have their own file if required. +As all the plots start +at the same position in x and have the same width, x0 and xlength are the +same for all options. Generally users will only want to change the start +level of the window y0 and its height ylength. + This option +allows users to change window positions whilst running the program. +The routine prompts first for the number of the option that the users +wishes +to reposition; then for the y start and height; then for the x start and +length. Note that changes to the x values affect all options. If the user +types only carriage return for any value it will remain unchanged. +Note that, unlike all the other programs, the boxes used to contain +analytical results (eg plot quality) should not be made to overlap one +another, as the function of the crosshair routine depends on which box the +crosshair is in! +.LEFT MARGIN1 +@15. TX 2 @Label a diagram +.LEFT MARGIN2 +.para +This routine allows users to label any diagrams they have produced. They +are asked to type in a label. When the user types carriage return to finish +typing the label the cross-hair appears on the screen. The user can +position it anywhere on the screen. If the user types R (for right justify) +the label will be +written on the diagram with its right end at the cross-hair position. +If the user types L (for left justify) the label will be written on the +diagram with its left end at the cross hair position. +The +cross-hair will then immediately reappear. The user may put the same +label +on another part of the diagram as before or if he hits the space bar he +will be asked if he wishes to type in another label. +.para +Typical dialogue follows. +.lit +? Menu or option number=15 +Type label then drive cross hair to left or right end +of label position then hit "L" to write label left +justified or "R" to write label right justified or +the space bar to quit + + +? Label=delta gene + + missing graphics + +? Label= + +.end lit +.left margin1 +@16. TX 2 @Display a map +.LEFT MARGIN2 +.para +This draws a map +of any sequence features selected by the user. +These features may be protein coding regions (CDS), tRNA genes (TRNA), +promoter positions (PRM), etc. Users may define their own feature table +key +names. For example I find it convenient to split CDS lines into CDS1, +CDS2 +and CDS3 each of which contains only those sequences that code in the +reading frames 1, 2 or 3. Then I can plot them at different heights on +the screen ( suitable heights can be determined by using the cross-hair). +The coordinates must be stored in a file in the format of an EMBL feature +table. +.para +Typical dialogue follows. +.lit +? Menu or option number=16 + Display a map using an EMBL feature table file +? map file name=hsegl1.ft +? feature code(e.g. CDS) =CDS +X 1 + strand + 2 - strand + 3 both strands +? 0,1,2,3 = +? level (0-9480) (256) =4000 + + missing graphics + +? feature code(e.g. CDS) = + +.end lit +.left margin1 +@7. TX 1 @Redirect output +.LEFT MARGIN2 +.para +Used to direct output that would normally appear on the screen to a file. +.para +Select redirection of either text or graphics, and +supply the name of the file that the output should be written to. +.para + The results from the next options selected will not appear on the screen +but will be written to the file. When option 7 is selected again +the file will be +closed and output will again appear on the screen. +.left margin1 +@13. TX 2 @Use crosshair +.left margin2 +.para +This option puts a steerable cross on the screen which the user +drives around +by using the arrow keys (or mouse). When the crosshair is +visible a number of options are available if the user types one of a +set of special keyboard characters. Any other characters will cause +an exit from the crosshair option. The special keys are: +.lit + + I = Identify the nearest gel reading + Z = Zoom in + Q = plot Quality + S = display the aligned Sequences at the crosshair position + N = list the Names and Numbers of the sequences at the crosshair +.end lit +.para +In order for any of these special keys to operate, the crosshair +must be in an appropriate display box, and the precise function of +the keys will also depend on which box the crosshair is in. +.para + If the +crosshair is in the "plot all contigs" box, Z will cause a new box to +appear showing all the readings for the nearest contig; Q will give +the same as Z but will also produce an extra box showing the +"quality" plot. +.para + If Z is hit in the "plot single contig" box, the contig will be zoomed +to the current graphics window size. The zoom will be roughly +centred on the crosshair position. Because of this it is possible to +step along a contig by repeatedly zooming with the crosshair near +to one end of the single contig display box. If I is hit the crosshair +must be close to a gel reading line. If Q is hit, the quality plot will +be produced for the region shown in the plot single contig box. In +all cases when the "plot all contigs" box is shown, a vertical line will +bisect the line the represents the relevant contig, at the current +position. +.para +If the crosshair is in the plot quality box only the character "s" will operate +as a special symbol. +.para +The number of bases shown in the N and S options is controlled by +the current graphics text window size, and the size of the zoom +window by the current graphics window size. Both are set by the +parameter setting function of the general menu. +.left margin1 +@33. TX 2 @Plot single contig +.left margin2 +.para +This option produces a schematic of a selected region of a single +contig by drawing a horizontal line to represent each of its gel +readings. The lines show the relative positions of each reading and +also their sense. The plot is divided vertically into two sections by +a line that is identified by an asterisk drawn at each end. All lines +that lie above this line represent readings that are in their original +sense, all lines below show readings that are in the +complementary sense to their original. By use of the crosshair +function the plot can be stepped through and examined in more +detail. See help on crosshair. +.left margin1 +@34. TX 2 @Plot all contigs +.left margin2 +.para +This option produces a schematic of all the contigs in a database. It +does this by drawing a horizontal line to represent each of them. +In order to show the ends of each contig it draws the lines for +contigs at alternate heights: the first at height one, the +second at height two, the third at height one, etc. The order of the +contigs in the display is the same as their order in the database. +By use of the crosshair function the plot can be stepped +through and examined in more detail. See help on crosshair. +.left margin1 +@31. TX 3 @ Type in gel readings +.left margin2 +.para +THIS OPTION IS NO LONGER AVAILABLE IN XDAP. +.para +This option allows gel readings to be typed in at the keyboard. It creates +a separate file for each gel reading and a file of file names for the +batch. The sequences from each batch may be listed when they have all been +entered. Users may choose to employ special keys to identify the 4 bases +A,C,G and T. By default these special keys are N M , . but any other four +characters may be used. If special keys are used the characters are +automatically translated to A C G T before being stored on the disk. + +.left margin1 +@35. TX 1 3 @Find internal joins +.left margin2 +.para +The purpose of this function is to use data already in the database to +find possible joins between contigs. +Joins may have been missed due to poor data or may have not been made +due to repeated sequences. Where appropriate, it may be +possible to find potential +joins by using the data clipped off readings prior to their entry into the +database. +.left margin2 +The database is checked for logical consistency. Supply a minimum initial +match length, a minimum alignment block, the maximum pads per sequence, +the maximum percent mismatch after alignment, the probe length. Choose +if clipped data is to be used, if so define the window size for finding good +data and the number of dashes allowed in the window. Processing will commence. +Most of these values are used in an identical way in the autoassemble +function. The others are defined below. +.left margin2 +The program strategy +.left margin2 +Take the first contig and calculate its consensus. If clipped data is being +used examine all readings that +are in the complementary orientation, and sufficiently near to the contigs left +end, to see if they have good clipped sequence which if present, would +protrude +from the left end of the contig. If found add the longest such sequence to the +left end of the consensus. Do the same for the right end by examining +readings that are in their +original orientation. If any are found add the longest extension to the +right end of +the consensus. Repeat the consensus calculations and extensions +for all contigs hence producing an extended consensus. If clipped data is not +being used simply calculate the consensus for the whole database. Now +look for possible joins by processing the extended consensus in the following +way. Take the last, say 100, bases (termed the "probe length" by the program) +of the rightmost consensus, compare it both +orientations with the extended consensus of all the other contigs. Display +any sufficiently good alignments. Repeat with the left end of the rightmost +contig. Do the same for the ends of all the entended contigs, always only +comparing with the contigs to their left, so that the same matches do not +appear twice. +.left margin2 +Good cliped data is defined by sliding a window of "Window size for good data +scan" bases outwards +along the sequence and stopping when "Maximum number of dashes in scan window" + or more dashes appear in the window. +Note that +it is advisable to have some sort of cutoff because if we simply take all the +data it might be so full of rubbish that we wont find any good matches. For +the same reason it is worth trying the procedure with different cutoffs. An +initial run using no clipped data is also recommended. +Sufficiently good +alignments are defined by criteria equivalent to those used in autoassemble, +however here we only display alignments that pass all tests. +.left margin2 +Bugs +.left margin2 +If a small contig is wholly contained within a larger one, such that its +ends are further than ("Probe length" - "Minimum initial match length") +from the ends of the larger contig, and the consensus for the small +contig lies to the left +of the consensus for large contig, the overlap will not be discovered. (See +the search stratgey). +.left margin2 + All numbering is +relative to base number one in the contig: matches to the left (i.e. in +the clipped data) have negative +positions, matches off the right end of the contig (i.e. in the clipped +data) have positions +greater than that of the contig length. +The convention for reporting the positions of overlaps is as follows: if neither +contig needs to be complemented the positions are as shown. If the program says +"contig x in the - sense" then the positions shown assume contig x has been +complemented. For example in the results given below the positions for the +first overlap are as reported, but those for the second assume that the contig +in the minus sense (i.e. 443) has been complemented. +.lit + + + Possible join between contig 445 in the + sense and contig 405 + Percentage mismatch after alignment = 4.9 + 412 422 432 442 452 462 + 405 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAG,TT AGCTCACTCA + ********* * ******** ***** *** ********** ********** ********** + 445 -TTCCCGACT G,AAAGCGGG TAGTGA,CGC AACGCAATTA ATGTGAG-TT AGCTCACTCA + -127 -117 -107 -97 -87 -77 + 472 482 492 502 512 + 405 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT AT + ********** ********** ********** ********** ** + 445 TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT AT + -67 -57 -47 -37 -27 + Possible join between contig 443 in the - sense and contig 423 + Percentage mismatch after alignment = 10.4 + 64 74 84 94 104 114 + 423 ATCGAAGAAA GAAAAGGAGG AGAAGATGAT TTTAAAAATG AAACG-CGAT GTCAGATGGG + **** ***** ********** ********** ****** ** ***** **** ********* + 443 ATCG,AGAAA GAAAAGGAGG AGAAGATGAT TTTAAA,,TG AAACGACGAT GTCAGATGG, + 3610 3620 3630 3640 3650 3660 + 124 134 144 154 164 + 423 TTG-ATGAAG TAGAAGTAGG AG-AGGTGGA AGAGAAGAGA GTGGGA + *** ****** ********** ** ******* *** ***** ** ** + 443 TTGGATGAAG TAGAAGTAGG AGGAGGTGGA ,GAG,AGAGA GTTGG- + 3670 3680 3690 3700 3710 + + +.end lit +.left margin1 +@ end of help diff --git a/help/GIP.RNO b/help/GIP.RNO new file mode 100644 index 0000000..d72dd68 --- /dev/null +++ b/help/GIP.RNO @@ -0,0 +1,205 @@ +.NPA +.left margin1 +.CENTER +GIP +.LEFT MARGIN1 +.PARA +A digitizer is + a two dimensional surface +which is such that if a special pen is pressed onto it, the pens +coordinates can be recorded by a computer. +These coordinates + can be interpreted by a program. +.para +The digitizing device we use works by the pen emitting a high frequency +sound which is picked up by two microphones positioned at the rear of the +working area. The pen position is determined by triangulation and the +digitizing device sends the coordinates to the computer. As no special +surface is required the device can conveniently be positioned on a light +box giving the sequencer an unobscured view of the autoradiographs. +.LEFT MARGIN1 +The digitizer + is called a GRAPHBAR MODEL GP7 made by + Science Accessories Corp, + 970 Kings Highway West, + Southport, + Connecticut 06490, + USA. + +.para + The program uses a menu to allow the user to select commands or + to enter the uncertainty codes for areas of the gel that are + difficult to interpret. A menu is simply a series of boxes drawn on + the digitizing surface that each contain a command or + uncertainty code. When the user puts the pen down in these special + regions the program interprets the coordinates as commands and acts + appropriately. A copy of the menu should have been sent to you. +It should be stuck down on the surface of the +light box in the digitizing area. For convenience it is best to position it +to the right of the digitizing area, but in practice as long as +its top +edge is parallel to the digitizer box, it can be put anywhere in the active +region. +.sk1 +.left margin1 + Entering gel readings using a digitizer +.left margin1 +.para +The autoradiograph should be stuck down on the light box with the lanes +running, as near is as +possible, at right angles to the digitizer. To read +an autoradiograph placed on the light box +the user need only define the positions of +the four sequencing lanes and the bases + to which they correspond and then use the pen to point to each + successive band progressing up the gel. The program examines the + coordinates of each pen position to see in which of the four +lanes + it lies and assigns the corresponding base to be stored in the + computer. Each time the pen tip is depressed to point to a position + on the surface of the digitizer the program sounds the bell on the + terminal (a different sound for each of the four bases on the +microcomputer version of the program) + to indicate to the user that a point has been recorded. As + the sequence is read the program displays it on the screen. + + +.para + The program uses a menu +to allow the user to select commands or + to enter the uncertainty codes for areas of the gel that are + difficult to interpret. A menu is simply a series of boxes drawn on + the digitizing surface that each contain a command or + uncertainty code. When the user puts the pen down in these special + regions the program interprets the coordinates as commands and acts + appropriately. As well as the uncertainty codes + A,C,G,T,1,2,3,4,B,D,H,V,R,Y,X,-,5,6,7,8 the following commands are + included in the menu: DELETE removes the last character from +the sequence; + RESET allows the lane centres to be redefined; +START means begin the next + stage of the procedure; STOP means stop the current stage in the + procedure; CONFIRM means confirm that the last command or set of + coordinates are correct. +.para +The digitizing device also has a menu of its own. This lies in a two inch wide +strip immediately in front of the digitizing box. Pen positions within this +two inch strip are interpretted as commands to the digitizer and are not +sent to the GIP program. In general the only time users will need to use +the device menu is when they tell GIP where the program menu lies in the +digitizing area. This is done by first hitting ORIGIN in the device menu +and then hitting the bottom left hand corner of the program menu. The +program menu can hence be positioned anywhere in the active region but +should be arranged parallel to the digitizer. +.para +The user should try to hit the bands as near as possible to the centre of +the lanes because the program tracks the lanes up the film using the pen +positions. By using this tracking strategy the user only has to define the +centres of the bottom of the lanes before starting to read the film. The +program can correctly follow quite curved lanes and constantly checks that +its lane centre coordinates look sensible. If the lane centres appear to be +getting too close the program stops responding to the pen positions of +bands and hence does not ring the bell. If this occurs users must hit the +reset box in the menu and the program will request them to redefine the +lane centres at the current reading position. Then they can continue +reading. As a further safeguard the program will only respond to pen +positions either in the menu or very close to the current reading position. +.sk1 +.left margin1 + Running the gel reading program +.left margin1 +The autoradiograph should be firmly stuck down on the light box and the +program started by typing GIP. It will ask the first question. +.left margin2 +" ? FILE OF FILE NAMES=" +.left margin2 +Type the name for the file of file names and then follow the instructions. +.left margin2 +" HIT DIGITIZER MENU ORIGIN" +.left margin2 +" THEN PROGRAM MENU ORIGIN" +.left margin2 +" THEN HIT START IN PROGRAM MENU" +.left margin2 +If the bell does not sound after you hit start try hitting metric in the +device menu (the program uses metric units, and some digitizers are set to +default to use inches; hitting metric switches between the two). +.left margin2 +After the bell has sounded the program will give the default lane order. +.left margin2 +" LANE ORDER IS T C A G" +.left margin2 +" IF CORRECT HIT CONFIRM, ELSE HIT RESET" +.left margin2 +If the lane order, reading from left to right is correct hit confirm in the +program menu. If you are using a different order hit reset and you will be +asked to define the lane order from left to right using the program menu +(as follows). +.left margin2 +" DEFINE LANE ORDER (LEFT TO RIGHT) USING MENU" +.left margin2 +Hit the boxes in the menu that contain the symbols A,C,G,T in the +left-right order of the lanes. The program will respond with the lane order +as above and ask for confirmation. When this is received, the next task is +to define the start positions of the next four lanes. +.left margin2 +" HIT START, THEN HIT (LEFT TO RIGHT)" +.left margin2 +" THE START POSITIONS FOR THE NEXT FOUR LANES" +.left margin2 +Hit the centres of the four lanes at a height level with the first band +that is going to be read. The program will report the mean lane separations +and asks for confirmation that they are correct. +.left margin2 +" MEAN LANE SEPARATION IS XX" +.left margin2 +" HIT CONFIRM TO CONTINUE" +.left margin2 +Users will become familiar with the values from their films and will spot +any unusual numbers. +Asking for confirmation allows users to try again if they had made a +mistake, but generally the lane separation values can be ignored. +Hit confirm, and the program will give the message +.left margin2 +" HIT START WHEN READY TO BEGIN READING" +.left margin2 +Hit start and the program will give the message +.left margin2 +" HIT BANDS, UNCERTAINTY CODES, RESET OR STOP" +.left margin2 +Hit the bands, interpretting the sequence progressing +up the film. If necessary use the uncertainty codes. If the pen stops +responding hit reset and follow the instructions as above. When the +sequence becomes unreadable hit stop and the program will ask for a file +name for the gel reading just read. +.left margin2 +" ? FILE NAME FOR THIS GEL READING=" +.left margin2 +Type the file name observing the rules about legal gel readings names. +The program will ask if you wish +to read another sequence. +.left margin2 +" TO ENTER ANOTHER GEL READING TYPE 1" +.left margin2 +To enter another type 1 and you will be back to the step of defining the +lane order. Typing anything else will stop the program. +.left margin1 +.sk1 +Running the microcomputer version of the gel reading program +.left margin1 +The microcomputer version of GIP is slightly different and is called +GIPB. The BBC micro +does not have the capacity to process the gel readings beyond the reading +stage. +This means that users of this program +would need to transfer their gel readings from the micro to another machine +using a terminal emmulator. Transferring many files is tedious and so the +microcomputer version of the gel reading program stores all the gel +readings for each run of the program in a single file. This special +file contains both sequences and file names and can be moved in a single +transfer to another machine. Once on the other machine the single file must +be split into separate gel reading files and a file of file names. This is +done using the program BSPLIT. As far as using the microcomputer version +of GIP, the only difference is that the first file name the program +requests is not a file of file names, but a name for the single file to +contain all the gel readings and their names. diff --git a/help/MEP.RNO b/help/MEP.RNO new file mode 100644 index 0000000..b3d575e --- /dev/null +++ b/help/MEP.RNO @@ -0,0 +1,859 @@ +.NPA +.SP 1 +.left margin1 +@-1. TX 0 @General +.sp +@-2. T 0 @Screen control +.sp +@-2. X 0 @Screen +.sp +@-3. TX 0 @Dictionary analysis +.sp +@0. TX -1 @MEP +.left margin2 +.para +This is a program for analysing families of nucleotide sequences in order +to find common motifs and potential binding sites. +The ideas in this program were described in Staden, R. "Methods +for discovering novel motifs in nucleic acid sequences". +Computer Applications in the Biosciences, 5, 293-298, (1989). +.PARA +The program can read +sequences stored in either of two formats: 1) all sequences aligned in a +single file; 2) all sequences in separate files and accessed through a file +of file names. +.PARA +The program contains functions that can answer several questions +about a set of sequences: +.SK1 +.left margin2 + Which words are most common? +.left margin2 + Which words occur in the most sequences? +.left margin2 + Which words contain the most information? +.left margin2 + Which words occur in equivalent positions in the sequences? +.left margin2 + Which words are inverted repeats? +.left margin2 + Which words occur on both strands of the sequences? +.left margin2 + Where are the inverted repeats? +.left margin2 + Where are the fuzzy words? +.para + Most of the program is +concerned with analysing +what it terms "fuzzy +words" within the set of sequences. The analysis is explained +below. Note that the standard version of the programs is limited +to words of maximum length 8 letters, and a maximum fuzziness +of 2. +.para +The following analyses (preceded by their option numbers) are included: +.lit + ? = Help + ! = Quit + 3 = Read new sequences + 4 = Redefine active region + 5 = List the sequences + 6 = List text file + 7 = Direct output to disk + 10 = Clear graphics + 11 = Clear text + 12 = Draw ruler + 13 = Use cross hair + 14 = Reset margins + 15 = Label diagram + 16 = Draw map + 17 = Search for strings + 18 = Set strand + 19 = Set composition + 20 = Set word length + 21 = Set number of mismatches + 22 = Show settings + 23 = Make dictionary Dw + 24 = Make dictionary Ds + 25 = Make fuzzy dictionary Dm from Dw + 26 = Make fuzzy dictionary Dm from Ds + 27 = Make fuzzy dictionary Dh from Dm + 28 = Examine fuzzy dictionary Dm + 29 = Examine fuzzy dictionary Dh + 30 = Examine words in Dm + 31 = Examine words in Dh + 32 = Save or restore a dictionary + 33 = Find inverted repeats +.end lit +.para +Some of these methods produce graphical + results +and so the +program is generally used from a graphics terminal (a vdu on which lines +and points can be drawn as well as characters). +.para +.LEFT MARGIN2 +The positions of each of the plots is defined relative to a users drawing +board which has size 1-10,000 in x and 1-10,000 in y. +Plots for +each option are drawn in a window defined by x0,y0 and xlength,ylength. +Where x0,y0 is the position of the bottom left hand corner of the window, + and xlength is the width of the window and ylength the +height of the window. +.lit + --------------------------------------------------------- 10,000 + 1 1 + 1 -------------------------------------- ^ 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 1 1 ylength 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 -------------------------------------- v 1 + 1 x0,y0^ 1 + 1 <---------------xlength--------------> 1 + --------------------------------------------------------- 1 + 1 10,000 + +.end lit +All values are in drawing board units (i.e. 1-10,000, 1-10,000). +The default window positions are read from a file "MEPMARG" when the +program is started. Users can have their own file if required. +.para +The options for the program are accessed from 3 main menus: general, screen +control and dictionary analylsis. +Both menus and options are selected by number. +.para +The most important and novel part of the program is its use of "fuzzy +dictionaries" and an information theory measure, to help show the most +interesting motifs. + + Central to the method is the idea of a fuzzy dictionary of word +frequencies. A dictionary of word frequencies is an ordered list of +all the words in the sequences and a count of the number of times +that they occur. A fuzzy dictionary is an equivalent list but which +contains instead, for each word, a count of the number of times +similar words occur in the sequences. We term words that are +similar "relations". The fuzziness is defined by the number of +letters in a word that are allowed to be different. So if we had a +fuzziness of 1 we allow 1 letter to be different. For example, with +a fuzziness of 1, the entry in the fuzzy dictionary for the word +TTTTTT would contain a count of the numbers of times TTTTTT +occured plus the number of times all words differing by exactly +one letter from TTTTTT occured. +.para + Once the fuzzy dictionary has been created we can examine it in +several ways to find candidate control sequences. The simplest +question we can ask is which word in the dictionary is the most +common. Sometimes this simple criterion of "most common" may +be adequate to discover a new motif but in general we would not +expect it to be sufficient. For example some words will be common +simply because of a base composition bias in the sequences being +analysed. In addition a word can be the most frequent and yet not +be "well defined". This last point is best explained by an example. +.para + Suppose we were looking at two letter words and allowing one +mismatch, and that there were 10 occurences of TT and 5 of AC. +We could align the 10 words that were one letter different from TT +and the 5 that were related to AC. Then we could count the +number of times each base occured in each position for each of +these two sets of words. Suppose we got the two base frequency +tables shown below. +.lit + TT AC + T 6 4 T 1 0 + C 1 3 C 0 4 + A 1 2 A 4 1 + G 2 1 G 0 0 + +.end lit +These tables show that although TT occurs (with one letter +mismatch) more often than AC, the ratio of base frequencies for +AC at 4/5, 4/5 is higher than those for TT at 6/10, 4/10. Hence we +would say that AC was better defined than TT. +Expressing this another way we would say that the definition of AC +contained more information than that for TT. The program +calculates the information content in a way that takes into account +both the sequence composition and the level of definition of the +motif. +.para +Definitions + +.para +Here we deal only with the dictionary analysis. +Suppose we are dealing with a set of +sequences and are examining them for words that are six +characters in length. + +.para +Dictionary Dw contains a count of the number of times each word +occurs in the set of sequences. For example the entry for TTTTTT +contains a value equal to the number of times the word TTTTTT +occurs in the set of sequences. + +.para +Dictionary Ds contains a count of the number of different sequences in +which each word occurs. For example if the entry for word TTTTTT +contains the value 10, it denotes that the word TTTTTT occurs in ten +different sequences. Unlike Dw it only counts words once for each +sequence. For example if we had a set of 100 sequences, the maximum +possible value that Ds could take is 100, and this would only happen if +a word occurred in every sequence. However for the same set of +sequences, Dw could contain values greater than 100, and this would +show that a word had occurred more than once in at least one +sequence. + +.para +From either of the two dictionaries Dw or Ds we can calculate a fuzzy +dictionary Dm. For each word, the entry in the fuzzy dictionary Dm +contains the sum of the dictionary values (taken from either Dw or Ds) +for all words that differ from it by up to m letters. For example if m=2 +the entry for TTTTTT contains the number of times that TTTTTT +occurs in the dictionary, plus the counts for all words that differ from +TTTTTT by 1 or 2 letters. +Obviously the interpretation of the values in Dm depends on which of +the two dictionaries Dw or Ds they were derived from. When derived +from Dw the entry for any word in Dm gives the total number of +times it, and its relations, occur in the set of sequences. When derived +from Ds the entry for any word in Dm gives the total number of +different sequences that contain a word and each of its relations. + +.para +Finally, from fuzzy dictionary Dm we can derive fuzzy dictionary Dh. +All entries in Dh are zero except for the word(s), within each set of +relations, that are most frequent. For example if TTTTTT occurred 20 +times but had a relation that occurred more often, then the entry for +TTTTTT would be zero. However if TTTTTT did not have a more +frequently occurring relation, then the entry for TTTTTT would +contain the value 20. + +.LEFT MARGIN1 +@1. T 0 @Help +.LEFT MARGIN2 +.para +This option gives online help. The user should select option numbers and +the current documentation will be given. Note that option 0 gives an +introduction to the program, and that ? will get help from anywhere in +the +program. +The following analyses (preceded by their option numbers) are included: +.lit + ? = Help + ! = Quit + 3 = Read new sequences + 4 = Redefine active region + 5 = List the sequences + 6 = List text file + 7 = Direct output to disk + 10 = Clear graphics + 11 = Clear text + 12 = Draw ruler + 13 = Use cross hair + 14 = Reset margins + 15 = Label diagram + 16 = Draw map + 17 = Search for strings + 18 = Set strand + 19 = Set composition + 20 = Set word length + 21 = Set number of mismatches + 22 = Show settings + 23 = Make dictionary Dw + 24 = Make dictionary Ds + 25 = Make fuzzy dictionary Dm from Dw + 26 = Make fuzzy dictionary Dm from Ds + 27 = Make fuzzy dictionary Dh from Dm + 28 = Examine fuzzy dictionary Dm + 29 = Examine fuzzy dictionary Dh + 30 = Examine words in Dm + 31 = Examine words in Dh + 32 = Save or restore a dictionary + 33 = Find inverted repeats +.end lit +.left margin1 +@2. T 0 @Quit +.left margin2 +.para +This function stops the program. +.left margin1 +@3. TX 1 @Read a new sequence +.LEFT MARGIN2 +.para +It can read +sequences stored in either of two formats: 1) all sequences aligned in a +single file; 2) all sequences in separate files and accessed through a file +of file names. Typical dialogue follows: +.lit + +X 1 Read file of aligned sequences + 2 Use file of file names +? 0,1,2 = + +? File of aligned sequences=F1 +Number of files 88 + +.end lit +.left margin1 +@4. TX 1 @Define active region +.LEFT MARGIN2 +.para +For its analytic functions +the program always works on a region of the sequence called the active +region. When new sequences are read into the program the active region is +automatically set to start at the beginning of the sequences and go +up to the end of the longest one. +.left margin1 +@5. TX 1 @List a sequence +.LEFT MARGIN2 +.para +The sequence can be listed with line lengths of 50 bases with each sequence +numbered in the order in which they were read. +Output can be directed to a disk file by +first selecting disk output. Typical dialogue follows. +.lit + +? Menu or option number=5 + + 10 20 30 40 50 + 1 TAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCA + 2 CAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTAC + 3 TAATTTATTCCATGTCACACTTTTCGCATCTTTGTTATGCTATGGTTATT + 4 ACTAATTTATTCCATGTCACACTTTTCGCATCTTTGTTATGCTATGGTTA + 5 AGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGA + 6 TAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGC + 7 ACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCG + 8 GGGGCAAGGAGGATGGAAAGAGGTTGCCGTATAAAGAAACTAGAGTCCGT + 9 AGGGGGTGGAGGATTTAAGCCATCTCCTGATGACGCATAGTCAGCCCATC + 10 AAAACGTCATCGCTTGCATTAGAAAGGTTTCTGGCCGACCTTATAACCAT + + 60 + 1 TACCCGTTTTT + 2 GCGTTTTTGT + 3 TCATACCATAAG + 4 TTTCATACC + 5 ATTGTGAGC + 6 TTCCGGCTCG + 7 GAAGAGAGT + 8 TCAGGTGT + 9 ATGAATG + 10 TAATTACG +.end lit +.left margin1 +@6. TX 1 @List a text file +.LEFT MARGIN2 +.para +Allows the user to have a text file displayed on the screen. It will appear +one page at a time. +.left margin1 +@7. TX 1 @Direct output to disk +.LEFT MARGIN2 +.para +Used to direct output that would normally appear on the screen to a file. +.para +Select redirection of either text or graphics, and +supply the name of the file that the output should be written to. +.para + The results from the next options selected will not appear on the screen +but will be written to the file. When option 7 is selected again +the file will be +closed and output will again appear on the screen. +.left margin1 +@10. TX 2 @Clear graphics +.LEFT MARGIN2 +.para + Clears the screen of both text and graphics. +.left margin1 +@11. TX 2 @Clear text +.LEFT MARGIN2 +.para + Clears only text from the screen. +.left margin1 +@12. TX 2 @Draw a ruler +.LEFT MARGIN2 +.para +This option +allows the user to draw a ruler or scale along the x axis of the screen to +help identify the coordinates of points of interest. The user can define +the position of the first amino acid to be marked (for example if the +active +region is 1501 to 8000, the user might wish to mark every 1000th amino +acid +starting at either 1501 or 2000 - it depends if the user wishes to treat +the active region as an independent unit with its own numbering starting +at +its left edge, or as part of the whole sequence). The user can also define +the separation of the ticks on the scale and their height. If required the +labelling routine can be used to add numbers to the ticks. +.left margin1 +@13. TX 2 @Use crosshair +.LEFT MARGIN2 +.para +This function puts +a steerable cross on the screen that can be used to find the +coordinates of points in the sequence. The user can move the cross +around using the directional keys; when he hits the space bar the +program will print out the coordinates of the cross in sequence units and +the option will be exited. +.para +If instead, +you hit a , the position will be displayed but the cross will remain on +the screen. +.para +If a letter s is hit the sequence around the cross hair is displayed and +the cross remains on the screen. +.left margin1 +@14. TX 2 @Reposition plots +.LEFT MARGIN2 +.para +The positions of each of the plots is defined relative to a users drawing +board which has size 1-10,000 in x and 1-10,000 in y. +Plots for +each option are drawn in a window defined by x0,y0 and xlength,ylength. +Where x0,y0 is the position of the bottom left hand corner of the window, + and xlength is the width of the window and ylength the +height of the window. +.lit + --------------------------------------------------------- 10,000 + 1 1 + 1 -------------------------------------- ^ 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 1 1 ylength 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 -------------------------------------- v 1 + 1 x0,y0^ 1 + 1 <---------------xlength--------------> 1 + --------------------------------------------------------- 1 + 1 10,000 + +.end lit +All values are in drawing board units (i.e. 1-10,000, 1-10,000). +The default window positions are read from a file "MEPMARG" when the +program is started. Users can have their own file if required. +As all the plots start +at the same position in x and have the same width, x0 and xlength are the +same for all options. Generally users will only want to change the start +level of the window y0 and its height ylength. + This option +allows users to change window positions whilst running the program. +The routine prompts first for the number of the option that the users +wishes +to reposition; then for the y start and height; then for the x start and +length. Note that changes to the x values affect all options. If the user +types only carriage return for any value it will remain unchanged. +The cross-hair can be used to choose suitable heights. +.LEFT MARGIN1 +@15. TX 2 @Label a diagram +.LEFT MARGIN2 +.para +This routine allows users to label any diagrams they have produced. They +are asked to type in a label. When the user types carriage return to finish +typing the label the cross-hair appears on the screen. The user can +position it anywhere on the screen. If the user types R (for right justify) + the label will be +written on the diagram with its right end at the cross-hair position. +If the user types L (for left justify) the label will be written on the +diagram with its left end at the cross hair position. +The +cross-hair will then immediately reappear. The user may put the same +label +on another part of the diagram as before or if he hits the space bar he +will be asked if he wishes to type in another label. +.left margin1 +@16. TX 2 @Display a map +.LEFT MARGIN2 +.para +It is often convenient to plot a map alongside graphed analysis in order +to +indicate features within the sequence. This function allows users to +draw +maps using files arranged in the form of EMBL feature tables. Of course +the +EMBL table are usually only used for nucleic acid sequence annotation +but, +as long as the features are written in the correct format, they can be +employed by this routine. The map is composed of a line representing the +sequence and then further lines denoting the endpoints of each feature +the +user identifies. The user is asked to define height at which the line +representing the sequence should be drawn; then for the feature height; +then for the features to plot. +.left margin1 +@17. TX 1 @Search for strings +.left margin2 +.para +Search for strings +perfoms searches of all the sequences for selected words and +shows which sequences they are found in. The user types in a word and +defines the allowed number of mismatches. The results are listed or +plotted. If listed the display includes the sequence number, the position +in the sequence and the matching string. +The results are plotted in the +following way. The x axis of the plot represents the length of the aligned +sequences and the y direction is divided into sufficient strips to accommodate +each sequence. So if a match is found in the 3rd sequence at a position +equivalent to halfway along the longest of the sequences then a short +vertical line will be drawn at the midpoint of the 3rd strip. If the sequences +are aligned it can be useful if the motifs happen to appear in +related positions. For example see the original publication. Typical +dialogue follows. +.lit + +? Menu or option number=17 +X 1 Plot match positions + 2 Plot histogram of matches +? 0,1,2 = +? Word to search for=TTGACA +? Minimum match (0-6) (6) =5 +? (y/n) (y) Plot results N + 2 35 TAGACA + 5 14 TTTACA + 6 37 TTTACA + 11 14 TAGACA + 14 14 TTGACA + 17 14 GTGACA + 17 22 TTAACA + 20 1 TTGACA +.end lit +.left margin1 +@18. TX 3 @Set strand +.left margin2 +.para +Set strand allows the user to define which strand(s) of the sequences to +analyse: input stand, complement of input, or both. +.left margin1 +@19. TX 3 @Set composition +.left margin2 +.para +Set composition gives the user three choices for setting the composition +of the sequences for use in the calculation of the information content of +words. The user can select the overall composition of the sequences as read, +an even composition, or can type in any other 4 values. +.left margin1 +@20. TX 3 @Set word length +.left margin2 +.para +Set word length sets the length of word for which dictionaries will be made. +.left margin1 +@21. TX 3 @Set number of mismatches +.left margin2 +.para +Set number of mismatches sets the level of fuzziness for the creation of +dictionary Dm. +.left margin1 +@22. TX 3 @Show settings +.left margin2 +.para +Show settings show the current settings for all parameters associated with +dictionary analysis. A typical diaplsy follows: +.lit + ? Menu or option number=22 + Current word length = 6 + Number of mismatches = 1 + Start position = 1 + End position = 63 + Input strand only + Observed composition + Dictionary Dw unmade + Dictionary Ds unmade + Dictionary Dm unmade + Dictionary Dh unmade +.end lit +.left margin1 +@23. TX 3 @Make dictionary Dw +.left margin2 +.para +Make dictionary Dw creates a dictionary that contains a count of the +frequency of occurrence of each word in the collected sequences. +.left margin1 +@24. TX 3 @Make dictionary Ds +.left margin2 +.para +Make dictionary Ds creates a dictionary that contains a count of the +number of different sequences that contain each word. +.left margin1 +@25. TX 3 @Make dictionary Dm from Dw +.left margin2 +.para +Make dictionary Dm from Dw creates a dictionary from dictionary Dw that +contains the frequency of occurrence of each word (say X) in Dw plus the +frequency of occurrence of each word in Dw that differs from X by up to m +letters. Dm is called a fuzzy dictionary as it contains the frequencies of +occurrence of all words plus the frequencies of all the words that are +similar to them. +.left margin1 +@26. TX 3 @Make dictionary Dm from Ds +.left margin2 +.para +Make dictionary Dm from Ds creates a dictionary from dictionary Ds that +contains the frequency of occurrence of each word (say X) in Ds plus the +frequency of occurrence of each word in Ds that differs from X by up to m +letters. Dm is called a fuzzy dictionary as it contains the frequencies of +occurrence of all words plus the frequencies of all the words that are +similar to them. +.left margin1 +@27. TX 3 @Make dictionary Dh from Dm +.left margin2 +.para +Make dictionary Dh creates a dictionary from dictionary Dm and whose +entries are zero except for those words in any set of related words that +are most frequent. It finds the dominant words in each set of relations +and stores their counts. +.left margin1 +@28. TX 3 @Examine fuzzy dictionary Dm +.left margin2 +.para +Examine dictionary Dm allows users to analyse the contents of dictionary +Dm to find the most common words or those words that contain the most +information. The user supplies a frequency or information cutoff and chooses +to have the results sorted on either value. The program will find the top 100 +words that achieve the cutoff values and present them to the user sorted +as selected. The information content will be calcutated from either Dw or Ds +depending which was used to create Dm, and using the current composition +setting. Typical dialogue follows: +.lit + +? Menu or option number=28 +Looking for highest scoring words +The highest word score = 115 +? Minimum word score (0-115) (0) =60 +? Minimum information (0.00-1.00) (0.00) =.62 +X 1 Sort on information + 2 Sort on word score +? 0,1,2 = + +? Maximum number to list (0-100) (100) = + +The words are + Total words= 9 Maximum information= 0.7385326 +TTGACA 60 0.73850 +AAAAAC 64 0.66460 +AAAAAA 90 0.64880 +GTTTTT 66 0.64300 +TTTTTG 73 0.64070 +TTTTGT 63 0.63820 +TTTTTC 65 0.63810 +AAAATA 63 0.62670 +TATAAT 65 0.62510 +The highest word score = 115 +? Minimum word score (0-115) (0) =60 +? Minimum information (0.00-1.00) (0.00) =.62 +X 1 Sort on information + 2 Sort on word score +? 0,1,2 =2 +? Maximum number to list (0-100) (100) = + +The words are + Total words= 9 Maximum information= 0.7385326 +AAAAAA 90 0.64880 +TTTTTG 73 0.64070 +GTTTTT 66 0.64300 +TTTTTC 65 0.63810 +TATAAT 65 0.62510 +AAAAAC 64 0.66460 +TTTTGT 63 0.63820 +AAAATA 63 0.62670 +TTGACA 60 0.73850 +The highest word score = 115 +? Minimum word score (0-115) (0) =! + +.end lit +.left margin1 +@29. TX 3 @Examine fuzzy dictionary Dh +.left margin2 +.para +Examine dictionary Dh allows users to analyse the contents of dictionary Dh +to find the most common words or those words that contain the most +information. The user supplies a frequency or information cutoff and chooses +to have the results sorted on either value. The program will find the top 100 +words that achieve the cutoff values and present them to the user sorted as +selected. The information content will be calcutated from either Dw or Ds +depending which was used to create Dh and using the current composition +setting. Typical dialogue follows: +.lit + +? Menu or option number=29 +Looking for highest scoring words +The highest word score = 115 +? Minimum word score (0-115) (0) =60 +? Minimum information (0.00-1.00) (0.00) =.6 +X 1 Sort on information + 2 Sort on word score +? 0,1,2 = + +? Maximum number to list (0-100) (100) = + +The words are + Total words= 4 Maximum information= 0.7385326 +TTGACA 60 0.73850 +AAAAAA 90 0.64880 +TATAAT 65 0.62510 +TTTTTT 115 0.60630 +The highest word score = 115 +? Minimum word score (0-115) (0) =50 +? Minimum information (0.00-1.00) (0.00) =.5 +X 1 Sort on information + 2 Sort on word score +? 0,1,2 = + +? Maximum number to list (0-100) (100) = + +The words are + Total words= 8 Maximum information= 0.7385326 +TTGACA 60 0.73850 +TCTTGA 54 0.66080 +AAAAAA 90 0.64880 +TATAAT 65 0.62510 +ACTTTA 57 0.61960 +TTTTTT 115 0.60630 +AGTATA 51 0.60540 +TTATAA 55 0.59300 +The highest word score = 115 +? Minimum word score (0-115) (0) =50 +? Minimum information (0.00-1.00) (0.00) = + +X 1 Sort on information + 2 Sort on word score +? 0,1,2 = + +? Maximum number to list (0-100) (100) = + +The words are + Total words= 8 Maximum information= 0.7385326 +TTGACA 60 0.73850 +TCTTGA 54 0.66080 +AAAAAA 90 0.64880 +TATAAT 65 0.62510 +ACTTTA 57 0.61960 +TTTTTT 115 0.60630 +AGTATA 51 0.60540 +TTATAA 55 0.59300 +The highest word score = 115 +? Minimum word score (0-115) (0) =! + +.end lit +.left margin1 +@30. TX 3 @Examine words in Dm +.left margin2 +.para +Examine words in Dm allows users to analyse the contents of dictonary Dm at the +level of individual words to find their frequency, information content, and to +see their base frequency table. The user types in a word to examine and the +program displays the values and table. The information content will be +calcutated from either Dw or Ds depending which was used to create Dm, +and using the current composition setting. Typical dialogue follows: +.lit +? Menu or option number=30 +? Word to examine=TTGACA +TtgacA 60 0.7385326 + 56 56 6 7 5 11 + 4 3 2 1 52 1 + 1 4 2 53 3 48 + 3 1 54 3 4 4 +TTGACA +? Word to examine=TATAAT +taTAat 65 0.6251902 + 56 3 53 4 4 60 + 6 1 5 5 5 3 + 3 60 5 57 57 4 + 4 5 6 3 3 2 +TATAAT +? Word to examine= + +.end lit +.left margin1 +@31. TX 3 @Examine words in Dh +.left margin2 +.para +Examine words in Dh allows users to analyse the contents of dictonary Dh at the +level of individual words to find their frequency, information content, and to +see their base frequency table. The user types in a word to examine and the +program displays the values and table. The information content will be +calcutated from either Dw or Ds depending which was used to create Dm, +and using the current composition setting. Typical dialogue follows: +.lit + + ? Menu or option number=31 +? Word to examine=TTGACA +TtgacA 60 0.7385326 + 56 56 6 7 5 11 + 4 3 2 1 52 1 + 1 4 2 53 3 48 + 3 1 54 3 4 4 +TTGACA +? Word to examine=TATAAT +taTAat 65 0.6251902 + 56 3 53 4 4 60 + 6 1 5 5 5 3 + 3 60 5 57 57 4 + 4 5 6 3 3 2 +TATAAT +? Word to examine=GGGGGG +gggggg 0 0.6199890 + 3 1 1 2 3 4 + 1 3 1 2 2 1 + 2 1 1 1 1 1 + 11 12 14 12 11 11 +GGGGGG +? Word to examine= + +.end lit +.left margin1 +@32. TX 3 @Save or restore a dictionary +.left margin2 +.para +Save or restore dictionary allows users to write or read any dictionary to +and from disk files. The user is asked te define the dictionary and file. The +function is useful if the machine being used is very slow at calculating +because the files can be handled quickly. However note that the files +cannot be processed by any other program. +.left margin1 +@33. TX 1 @Find inverted repeats +.left margin2 +.para +Find inverted repeats performs searches for simple inverted repeat sequences +in each sequence. They are defined by a range of loop sizes and a minimum +number of potential basepairs. The results can be plotted or listed. The x +axis of the plot represents the length of the aligned sequences and the y +direction is divided into sufficient strips to accommodate each sequence. +So if an inverted repeat is found in the 3rd sequence at a position equivalent +to halfway along the longest of the sequences then a short vertical line will +be drawn at the midpoint of the 3rd strip. Alternatively, if the results are +listed, the potential hairpin loops are drawn out, with the sequence number +and the position of the loop. Typical dialogue follows. +.lit + +? Menu or option number=33 +Define the range of loop sizes +? Minimum loop size (0-10) (3) =0 +? Maximum loop size (1-20) (3) = +? Minimum number of basepairs (1-20) (6) = +? (y/n) (y) Plot results N + Searching + +Sequence 3 34 + C + G.T + T-A + A-T + T.G + T.G + G.T + ATCTTT TATTTCA + 33 + +Sequence 5 35 + T + G.T + T.G + A-T + T.G + G.T + C-G + T.G + TCCGGC AATTGTG + 34 +.end lit +.left margin1 +@ End of help diff --git a/help/NIP.RNO b/help/NIP.RNO new file mode 100644 index 0000000..6db18e6 --- /dev/null +++ b/help/NIP.RNO @@ -0,0 +1,5116 @@ +.NPA +.SP 1 +.left margin1 +@-1. TX 0 @General +.sp +@-2. T 0 @Screen control +.sp +@-2. X 0 @Screen +.sp +@-3. T 0 @Statistical analysis of content +.sp +@-3. X 0 @Statistics +.sp +@-4. T 0 @Structures and repeats +.sp +@-4. X 0 @Structures +.sp +@-5. TX 0 @Translation and codons +.sp +@-6. TX 0 @Gene search by content +.sp +@-7. TX 0 @General signals +.sp +@-8. TX 0 @Specific signals +.sp +@0. TX -1 @NIP +.PARA +.para +This is a program for analysing individual nucleotide sequences. It can +read sequences stored in many of the most commonly used formats, and +performs all of the usual simple analyses. However the main purpose of +the program is to provide methods for finding the function of each +section of a sequence. In general no single method can give an +unequivecal interpretation of a sequence so we need to use many +techniques together and to combine their results. For this reason the +program present many of its results graphically. +.para +General information is contained in the user interface. Online +documentation for any function follows a consistent pattern: summary, +list of inputs, list of outputs, details, example. +.LEFT MARGIN1 +@1. TX 0 @ Help +.LEFT MARGIN2 +.para +This option gives online help. The user should select option numbers and +the current documentation will be given. Note that option 0 gives an +introduction to the program, and that ? will get help from anywhere in +the +program. +The following functions are included: +.left margin1 +@2. TX 0 @ Quit +.left margin2 +.para +This function stops the program. +.left margin1 +@3. TX 1 @ Read a new sequence +.LEFT MARGIN2 +.para +This option allows users to read in new sequences, browse through annotations, + or search sequence +libraries for keywords. Sequences can be read from "personal" +sequence files or from sequence libraries. These are referred to as the +sequence "source". Personal files can be stored in several formats: +Staden, PIR, EMBL, GENBANK and GCG. +At LMB we use "Staden" format for sequencing and all +the +libraries are stored in their original formats. Note, however, that libraries +such as EMBL or GenBank that are divided into several files (eg GenBank has +13 separate files) are indexed as a whole. This means that users do not need +to know which file contains an entry, only which library. +When the user selects to read in a sequence the program first asks for the +sequence "source". +.para +If the user selects "personal" the program will ask for +the format (Staden, PIR, EMBL, GENBANK or GCG), and then for the name of +the file. For PIR format the user will also be required to know the entry +name of the sequence as the file can contain several. For the other formats +only a single entry is expected. The file will be read, its length and +composition will be displayed and the option left. +.para +If the user selects "library" as the sequence source the program will display a +list of available libraries. The programs are capable of handling all current +libraries but which ones are available will vary from site to site. At LMB we +have several libraries and also weekly updates of data gathered between releases. +The program will ask users to select a library and then give a list of options: +.lit + + X 1 Get a sequence + 2 Get annotations + 3 Get entrynames from accession numbers + 4 Search titles for keywords + 5 Search text index for keywords + +.end lit +If get a sequence or get annotations is selected users will be asked to +type the entry name. The option will be left when a sequence is selected or +! is typed. The composition and length will be displayed. +.para +The text index contains all words from feature tables, reference titles, +definition lines, keywords lists and comments, so the text index search +is most useful. It is also the fastest. Up to 5 words can be searched for +at once. The words should be typed separated by spaces, for example +.lit + ? Keywords=P53 mouse murine tumo + +.end lit +will search for all entries that contain words starting with p53, mouse, +murine and tumo. Only the unique entries that contain ALL words will be +listed. Before listing the matching entries +the program will show the number of 'hits' for each word and ring the bell. +Escape is possible at this point, or after each screenfull of entries. +In addition to the entry names the text search displays the primary accession +number, the sequence length and up to 80 characters of description. +(The search of 'titles' is now redundant because the full text index +contains all the title words and the search is much faster. It will probably +be removed from the program.) +All searches are independent of case. Where +possible the program will offer default entry names. +.para +Typical dialogue follows. +.lit +Select sequence source +X 1 Personal file + 2 Sequence library +? Selection (1-2) (1) = +Select sequence file format +X 1 Staden + 2 EMBL + 3 GenBank + 4 PIR + 5 GCG +? Selection (1-5) (1) = +? Sequence file name=M13MP7.SEQ + Contig title removed +Sequence length= 7238 + Sequence composition + T C A G - + 2405. 1539. 1765. 1527. 2. + 33.2% 21.3% 24.4% 21.1% 0.0% + . + . + . + + + Select sequence source + X 1 Personal file + 2 Sequence library + ? Selection (1-2) (1) =2 + Select a library + X 1 EMBL 29 nucleotide library Dec 91 + 2 SWISSPROT 20 protein library Nov 91 + 3 PIR 31 protein library Dec 91 + 4 NRL3D 58 From Brookhaven protein library Dec 91 + 5 GenBank + ? Selection (1-5) (1) = +Library is in EMBL format with indexes + Select a task + X 1 Get a sequence + 2 Get annotations + 3 Get entry names from accession numbers + 4 Search titles for keywords + 5 Search text index for keywords + ? Selection (1-5) (1) =5 + Search for keywords + ? Keywords=P53 mouse +P53 hits 68 +MOUSE hits 8180 + + MMANT01 X00875 536 Murine gene fragment for cellular tumour antigen + MMANT02 X00876 83 Murine gene fragment for cellular tumour antigen + MMANT03 X00877 21 Murine gene fragment for cellular tumour antigen + MMANT04 X00878 261 Murine gene fragment for cellular tumour antigen + MMANT05 X00879 184 Murine gene fragment for cellular tumour antigen + MMANT06 X00880 113 Murine gene fragment for cellular tumour antigen + MMANT07 X00881 110 Murine gene fragment for cellular tumour antigen + MMANT08 X00882 137 Murine gene fragment for cellular tumour antigen + MMANT09 X00883 74 Murine gene fragment for cellular tumour antigen + MMANT10 X00884 107 Murine gene for cellular tumour antigen p53 (exon + MMANT11 X00885 562 Murine p53 gene 3' region with exon 11 + MMANTP53 M26862 536 Mouse tumor antigen p53 gene, 5' end. + MMLYN M64608 2044 Mouse lyn protein mRNA, complete cds. + MMP53 X00741 1377 Mouse mRNA for transformation associated protein + MMP53A M13872 1285 Mouse p53 mRNA, complete cds, clone pcD53. + MMP53B M13873 1241 Mouse p53 mRNA, complete cds, clone p53-m11. + MMP53C M13874 1322 Mouse p53 mRNA, complete cds, clone p53-m8. + MMP53G1 X01235 554 Mouse genomic DNA for 5' region of cellular tumou + MMP53IN4 X60470 729 M.musculus p53 gene for p53 protein, intron 4 + MMP53P X01236 2132 Mouse pseudogene for cellular tumour antigen p53 + MMP53R X01237 1773 Mouse mRNA for cellular tumour antigen p53 + MMRSB2P5 M64597 196 Mouse B2 repeat in the 3' flank of protein 53 (p5 + 22 different entries found + + Select a task + X 1 Get a sequence + 2 Get annotations + 3 Get entry names from accession numbers + 4 Search titles for keywords + 5 Search text index for keywords + ? Selection (1-5) (1) =4 + Search for keywords + ? Keywords=alpha + Searching for alpha + AAGHA 623 a.anguilla mrna for glycoprotein hormone alpha subunit precu + AAMALI 3338 a.aegypti mali gene encoding alpha 1-4 glucosidase, complete + AAMALIA 1659 a.aegypti maltase-like i (mali) gene encoding alpha-1,4-gluc + AAMALIB 1832 a.aegypti maltase-like i (mali) mrna encoding alpha-1,4-gluc + ACA13GT 371 alouatta caraya alpha-1,3gt gene, 3' flank. + ADHBADA1 102 duck alpha-d-globin gene, exon 1. + ADHBADA2 1145 duck alpha-a-globin gene and 5' flank + ADHBADWP 513 duck (white pekin) alpha ii (minor) globin mrna, complete co + AEACOXABC 5279 a.eutrophus protein x (acox), acetoin:dcpip oxidoreductase-a + AGA13GT 371 ateles geoffroyi alpha-1,3gt gene, 3' flank. + AGAAAGFP 282 c.tetragonoloba alpha-amylase/alpha-galactosidase fusion pro + AGAABL 138 b.subtilis alpha-amylase signal peptide gene e.coli beta-lac + AGAFAMYA 57 synthetic b.stearothermophilus alpha amylase/s.cerevisiae ma + AGAFAMYB 57 synthetic b.stearothermophilus alpha amylase/s.cerevisiae ma + AGAFAMYC 57 synthetic b.stearothermophilus alpha amylase/s.cerevisiae ma + AGAFCOXA 98 synthetic alpha-factor/cox iv fusion gene signal peptide. + AGAGABA 7876 synthetic gossypium hirsutum (cotton) alpha globulin a and b + AGAMYLS 120 synthetic alpha-amylase gene, 5' end. + AGANPS 95 synthetic gene (jcnf-1) encoding alpha-factor pro-region/han +! + Select a task + X 1 Get a sequence + 2 Get annotations + 3 Get entry names from accession numbers + 4 Search titles for keywords + 5 Search text index for keywords + ? Selection (1-5) (1) =3 + ? Accession number=v00636 +Entry name LAMBDA + Select a task + X 1 Get a sequence + 2 Get annotations + 3 Get entry names from accession numbers + 4 Search titles for keywords + 5 Search text index for keywords + ? Selection (1-5) (1) =2 + Default Entry name=LAMBDA + ? Entry name= +ID LAMBDA standard; DNA; PHG; 48502 BP. +XX +AC V00636; J02459; M17233; X00906; +XX +DT 03-JUL-1991 (Rel. 28, Last updated, Version 3) +DT 09-JUN-1982 (Rel. 1, Created) +XX +DE Genome of the bacteriophage lambda (Styloviridae). +XX +KW circular; coat protein; DNA binding protein; genome; +KW origin of replication. +XX +OS Bacteriophage lambda +OC Viridae; ds-DNA nonenveloped viruses; Siphoviridae. +XX +RN [1] +RP 1-48502 +RA Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B.; +RT "Nucleotide sequence of bacteriophage lambda DNA"; +RL J. Mol. Biol. 162:729-773(1982). +XX +! + Select a task + X 1 Get a sequence + 2 Get annotations + 3 Get entry names from accession numbers + 4 Search titles for keywords + 5 Search text index for keywords + ? Selection (1-5) (1) = + Default Entry name=LAMBDA + ? Entry name= +DE Genome of the bacteriophage lambda (Styloviridae). + Sequence length 48502 + Sequence composition + T C A G - + 11988. 11360. 12336. 12818. 0. + 24.7% 23.4% 25.4% 26.4% 0.0% + +.end lit +.left margin1 +@4. TX 1 @ Define active region +.LEFT MARGIN2 +.para +For its analytic functions +the program always works on a region of the sequence called the "active +region". This function allows the start and end points of the active region +to be reset. +.para +Define the required start and end points. +.para +When a new sequence is read into the program the active region is +automatically set to start at the beginning of the sequence and extend to +the +maximum the program can +handle. On most machines this will be to the end of the sequence. The +positions are shown on the screen. + Note that for +convenience, in the +listing and translation functions, the user is given access to regions +outside the active region. +.left margin1 +@5. TX 1 @ List a sequence +.LEFT MARGIN2 +.para +The sequence can be listed single or double stranded with line lengths +from +10 to 120 in multiples of 10. +.para +Define the region to list, the line length required and choose between a +single or double stranded display. +The output looks like: +.lit + + GTTAATGTAG CTTAATAACA AAGCAAAGCA CTGAAAATGC TTAGATGGAT + CAATTACATC GAATTATTGT TTCGTTTCGT GACTTTTACG AATCTACCTA + 10 20 30 40 50 + + AATTGTATCC CATAAACACA AAGGTTTGGT CCTGGCCTTA TAATTAATTA + TTAACATAGG GTATTTGTGT TTCCAAACCA GGACCGGAAT ATTAATTAAT + 60 70 80 90 100 + + GAGGTAAAAT TACACATGCA AACCTCCATA GACCGGTGTA AAATCCCTTA + CTCCATTTTA ATGTGTACGT TTGGAGGTAT CTGGCCACAT TTTAGGGAAT + 110 120 130 140 150 + + AACATTTACT TAAAATTTAA GGAGAGGGTA TCAAGCACAT TAAAATAGCT + TTGTAAATGA ATTTTAAATT CCTCTCCCAT AGTTCGTGTA ATTTTATCGA + 160 170 180 190 200 + +.end lit +.left margin1 +@6. TX 1 @ List a text file. +.LEFT MARGIN2 +.para +Allows the user to have a text file displayed on the screen. It will appear +one page at a time. +.para +Supply the name of the file to be displayed. +.left margin1 +@7. TX 1 @ Direct output to disk +.LEFT MARGIN2 +.para +Used to direct output that would normally appear on the screen to a file. +.para +Select redirection of either text or graphics, and +supply the name of the file that the output should be written to. +.para + The results from the next options selected will not appear on the screen +but will be written to the file. When option 7 is selected again +the file will be +closed and output will again appear on the screen. +.left margin1 +@8. TX 1 @ Write active region to disk +.LEFT MARGIN2 +.para +Used to write the current active section of sequence to a disk file in +"Staden format". +.para +Supply a file name and an optional title. +.para +The program has the capability of reading sequences stored in several +formats and so, in conjunction with this option, can be used to reformat +them. +.left margin1 +@9. TX 1 @ Edit the sequence +.LEFT MARGIN2 +.para +Used to edit sequences or any other files by giving access to the +computers system editor. For editing sequences the input file should +have already been created using one of the listing functions such as "list +sequence", "list translation" or "list restriction sites above the +sequence". +.para +Supply the name of the file to edit. Wait while the system editor is made +ready (can take awhile on a vax). Use the editor. Exit from the editor. If a +sequence has been edited, and you want to process it, affirm that the +sequence should be "made active". The edited sequence will replace the +original sequence. +.para +This editing method is designed to give users access to an editor with +which they are familiar - i.e. the one on their machine, and yet to allow +them to edit a sequence which contains all the landmarks they need in +order to know where they are. Users can create files containing simple +listings (single stranded) with numbering, using "list the sequence", and +then edit them with their system editor, using the numbering to know +where they are within the sequence. When the edits are complete they +exit from the editor and the program "analyses" the edited file to extract +only the sequence characters. Similarly a file containing a three phase +tranlslation can be edited, or a file containing a sequence plus its three +phase translation, plus its restriction sites marked above the sequence. +In order to be able to "analyse" such complicated listings and correctly +extract the sequence the following simple rule is used: all lines in the +file that contain a character that is not A,C,T,G or U are deleted. It is +obviously important to be aware of this rule and its implications. +.left margin1 +@10. TX 2 @ Clear graphics +.LEFT MARGIN1 +.para + Clears graphics from the screen. +.left margin1 +@11. TX 2 @ Clear text +.LEFT MARGIN1 +.para + Clears text from the screen. +.left margin1 +@12. TX 2 @ Draw a ruler +.LEFT MARGIN2 +.para +This option +allows the user to draw a ruler or scale along the x axis of the screen to +help identify the coordinates of points of interest. The user can define +the position of the first base to be marked (for example if the active +region is 1501 to 8000, the user might wish to mark every 1000th base +starting at either 1501 or 2000 - it depends if the user wishes to treat +the active region as an independent unit with its own numbering starting +at +its left edge, or as part of the whole sequence). The user can also define +the separation of the ticks on the scale and their height. If required the +labelling routine can be used to add numbers to the ticks. +.left margin1 +@13. TX 2 @ Use crosshair +.LEFT MARGIN2 +.para +This function puts +a steerable cross on the screen that can be used to find the +coordinates of points in the sequence. The user can move the cross +around using the directional keys; when he hits the space bar the +program will print out the coordinates of the cross in sequence units and +the option will be exited. +.PARA +If instead, +you hit a , the position will be displayed but the cross will remain on +the screen. +.PARA +If a letter s is hit the program will display the sequence around the +crosshair +position, and leave the cross on the screen. +.left margin1 +@14. TX 2 @ Reposition plots +.LEFT MARGIN2 +.para +The positions of each of the plots is defined relative to a users drawing +board which has size 1-10,000 in x and 1-10,000 in y. +Plots for +each option are drawn in a window defined by x0,y0 and xlength,ylength. +Where x0,y0 is the position of the bottom left hand corner of the window, + and xlength is the width of the window and ylength the +height of the window. +.lit + --------------------------------------------------------- 10,000 + 1 1 + 1 -------------------------------------- ^ 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 1 1 ylength 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 -------------------------------------- v 1 + 1 x0,y0^ 1 + 1 <---------------xlength--------------> 1 + --------------------------------------------------------- 1 + 1 10,000 + +.end lit +All values are in drawing board units (i.e. 1-10,000, 1-10,000). +The default window positions are read from a file "NIPMARG" when the +program is started. Users can have their own file if required. +As all the plots start +at the same position in x and have the same width, x0 and xlength are the +same for all options. Generally users will only want to change the start +level of the window y0 and its height ylength. + This option +allows users to change window positions whilst running the program. +The routine prompts first for the number of the option that the users +wishes +to reposition; then for the y start and height; then for the x start and +length. Note that changes to the x values affect all options. If the user +types only carriage return for any value it will remain unchanged. +The cross-hair can be used to choose suitable heights. +.LEFT MARGIN1 +@15. TX 2 @ Label a diagram +.LEFT MARGIN2 +.para +This routine allows users to label any diagrams they have produced. They +are asked to type in a label. When the user types carriage return to finish +typing the label the cross-hair appears on the screen. The user can +position it anywhere on the screen. If the user types R (for right justify) +the label will be +written on the diagram with its right end at the cross-hair position. +If the user types L (for left justify) the label will be written on the +diagram with its left end at the cross hair position. +The +cross-hair will then immediately reappear. The user may put the same +label +on another part of the diagram as before or if he hits the space bar he +will be asked if he wishes to type in another label. +.para +Typical dialogue follows. +.lit +? Menu or option number=15 +Type label then drive cross hair to left or right end +of label position then hit "L" to write label left +justified or "R" to write label right justified or +the space bar to quit + + +? Label=delta gene + + missing graphics + +? Label= + +.end lit +.left margin1 +@16. TX 2 @Display a map +.LEFT MARGIN2 +.para +This draws a map +of any sequence features selected by the user. +These features may be protein coding regions (CDS), tRNA genes (TRNA), +promoter positions (PRM), etc. Users may define their own feature table +key +names. For example I find it convenient to split CDS lines into CDS1, +CDS2 +and CDS3 each of which contains only those sequences that code in the +reading frames 1, 2 or 3. Then I can plot them at different heights on +the screen ( suitable heights can be determined by using the cross-hair). +.para +The coordinates must be stored in a file in the format of an EMBL or GenBank +feature table. Note that this means that the file must include either EMBL +or GenBank headers, and a suitable "tail". The simplest header is the word +FEATURES starting in column 1 of the first line of the file. The simplest +tail is 2 empty lines at the end of the file. These lines are not included +when nip writes out results in feature table format. +.para +Typical dialogue follows. +.lit +? Menu or option number=16 + Display a map using an EMBL feature table file +? map file name=hsegl1.ft +? feature code(e.g. CDS) =CDS +X 1 + strand + 2 - strand + 3 both strands +? 0,1,2,3 = +? level (0-9480) (256) =4000 + + missing graphics + +? feature code(e.g. CDS) = + +.end lit +.left margin1 +@17. TX 1 @ Search for restriction enzymes +.LEFT MARGIN2 +.para +This routine is used to search for short sequences, like restriction +enzyme +recognition sequences, +and can either list the results or present them graphically. Listings can +take several forms and can include the sequence and its translation. +Examples are given below. The program will also display the names of +enzymes that cut the sequence infrequently. Users can select from sets +of enzymes stored in files or can enter them from the keyboard. +.para +The short +sequences (strings) and their names need to be arranged in a particular +way. See below. Select to search, list an enzyme file or clear the screen. +Choose either a file of enzymes or to enter their recognition sequences at the +keyboard. Choose to search for all the enzymes in the list or to select +from the list. Select a mode of output. Define the sequence as circular or +linear. Select to search for "definite" or "possible" matches. The search +starts, and after the results have been displayed, further searches can be +performed. +.para +When the enzymes and their recognition sequences are stored in a file +they must be defined in the following way. We +call the recognition sequences "strings". +The format is as follows: each string or set of strings must be +preceded by a name, each string must be preceded and +terminated with a slash (/), and +each set of strings by 2 slashes. +For example +AATII/GACGT'C// defines the name AATII, its recognition sequence +GACGTC +and its cut site with the ' symbol; ACCI/GT'MKAC// defines the name +ACCI +and its recognition sequence includes IUB symbols for incompletely +defined +symbols in nucleic acid sequences; +BBVI/GCAGCNNNNNNNN'/'NNNNNNNNNNNNGCTGC// +defines the name BBVI and this time two recognition sequences and cut +sites +are specified in order to correctly show the cutting position relative to +the recognition sequence. If no cut site is included the first base of the +recognition sequence is displayed as being on the 3' side of the +recognition sequence. +.para +These collections of strings and their +names can be read from disk or entered from the keyboard. +When names and strings are entered from the keyboard the program will ask +for the name and then the string(s). If more than one string is typed per +name they must be separated by slash (/) characters. See the "Typical +dialogue" below. + Three files +containing restriction enzyme recognition sequences are currently +available. The "all enzymes" file contains the Rich Roberts REBASE +restriction enzyme database, which is updated monthly. +.para +The user can select strings +by name from these collections. If so the program will prompt for the +names, one at a time. The user can continue to select names until a blank +name is entered (by the user typing only return). +.para + Listed output can be displayed in several ways: it +can be ordered enzyme by enzyme, or on cut positions, or with enzyme +names +written above a listing of the sequence. This last listing can also include +a three phase translation of the sequence. In addition the program will +display only infrequent cutters (the user defines the minimum number of +cuts), or can plot the positions of matches. +.para +Listings sorted "enzyme by enzyme" have the following form: +.lit + + Matches found= 1 + Name Sequence Position Fragment lengths + 1 AATII GACGT'C 112 111 111 + 912 912 + Matches found= 2 + Name Sequence Position Fragment lengths + 1 ACCI GT'CGAC 112 111 111 + 2 ACCI GT'AGAC 420 308 308 + 604 604 + Matches found= 2 + Name Sequence Position Fragment lengths + 1 AHAII GA'CGTC 109 108 90 + 2 AHAII GG'CGTC 199 90 108 + 825 825 + Matches found= 2 + Name Sequence Position Fragment lengths + 1 AVAII G'GACC 84 83 51 + 2 AVAII G'GTCC 973 889 83 + 51 889 + Matches found= 1 + Name Sequence Position Fragment lengths + 1 BALI TGG'CCA 258 257 257 + 766 766 + Matches found= 1 + Name Sequence Position Fragment lengths + 1 BAMHI G'GATCC 92 91 91 + + ...... etc + +Listings sorted on cut position have the following form: + + Searching + Name Sequence Position Fragment lengths + 1 ECORI G'AATTC 2 1 + 2 BANI G'GTGCC 26 24 + 3 BSP1286 GTGCC'C 31 5 + 4 BBVI 'TACTGCGCCGCAGCTGC 38 7 + 5 NSPBII CAG'CTG 51 13 + 6 PVUII CAG'CTG 51 0 + 7 BBVI GCAGCTGCTGGTG' 60 9 + 8 HINCII GTC'AAC 80 20 + 9 AVAII G'GACC 84 4 + 10 BINI 'CCAGGGATCC 87 3 + 11 BSTNI CC'AGG 89 2 + 12 BAMHI G'GATCC 92 3 + 13 XHOII G'GATCC 92 0 + 14 NSPBII CCG'CTG 98 6 + 15 BINI GGATCCGCT' 100 2 + 16 AHAII GA'CGTC 109 9 + 17 SALI G'TCGAC 111 2 + 18 AATII GACGT'C 112 1 + 19 ACCI GT'CGAC 112 0 + 20 HINCII GTC'GAC 113 1 + 21 BBVI GCAGCGACTGATT' 166 53 + 22 BINI 'ACTCAGATCC 178 12 + 23 XHOII A'GATCC 183 5 + 24 HGAI 'GGCGGCGGAGGCGTC 188 5 + + .....etc + +Lists of infrequent cutters have the following form: + + 0 AFLII + 0 AFLIII + 0 APAI + 0 APALI + 0 ASUII + 0 AVAI + 0 AVRII + 0 BCLI + 0 BGLI + 0 BGLII + 0 BSMI + 0 BSPMII + 0 BSTEII + ...... etc + + Listings showing names above the sequence, and a translation have the +following form: + + + ECORI BANI BSP1286 + . . . BBVI NSPBII + . . . . PVUII BBVI +GAATTCGGTTTGGGCTTGGTGTGAGGTGCCCAGAGATTACTGCGCCGCAGCTGCTG +GTGC + 10 20 30 40 50 60 + E F G L G L V * G A Q R L L R R S C W C + N S V W A W C E V P R D Y C A A A A G A + I R F G L G V R C P E I T A P Q L L V L + + HINCII + . AVAII + . . BINI + . . . BSTNI + . . . . BAMHI + . . . . XHOII NSPBII + . . . . . . BINI AHAII + . . . . . . . . SALI + . . . . . . . . .AATII + . . . . . . . . .ACCI + . . . . . . . . ..HINCII +TGGCGGTGCGGAGGTCGTCAACGGACCCAGGGATCCGCTGGACGAGGACGTCGACG +ACGA + 70 80 90 100 110 120 + W R C G G R Q R T Q G S A G R G R R R R + G G A E V V N G P R D P L D E D V D D E + A V R R S S T D P G I R W T R T S T T R + + BBVI BINI +GGAGGAGGTGGATAGCGCATTGCTGGTGGCTGGCAGCGACTGATTTGAGTTCTGAC +CACT + 130 140 150 160 170 180 + G G G G * R I A G G W Q R L I * V L T T + E E V D S A L L V A G S D * F E F * P L + R R W I A H C W W L A A T D L S S D H S + + XHOII + . HGAI AHAII PFIMI + . . . . BBVI +CAGATCCGGCGGCGGAGGCGTCGAGGCTCCCGAAACTCCCAGTGGCTGGCCTGCTA +GATT + 190 200 210 220 230 240 + Q I R R R R R R G S R N S Q W L A C * I + R S G G G G V E A P E T P S G W P A R F + D P A A E A S R L P K L P V A G L L D S + + .........etc + +.end lit +.para +The terms "possible" and "definite" matches are important only for back +translations of protein into DNA, and which include IUB redundancy codes. +Those matches that the program terms "definite matches" and are ones in +which the specification of the recognition sequence corresponds +exactly to that of the back translation, and consequently are definitely in +the DNA sequence. The program will also find what it +terms 'possible matches' which are ones that depend on the particular +codons +chosen for each amino acid. +These are sites at which recognition +sequences could be engineered to produce a cut in the DNA +without changing the amino +acid, but which are not +necessarily found in the original sequence. +.para +The routine will handle both linear and circular sequences, and +so finds cutsites spanning the "ends" of circular sequences. + The program will only find cutsites spanning the +ends of sequences if the sequence is declared as circular. +This includes sites for +recognition sequences containing leading or trailing N symbols, in which +the actual recognition sequence does not span the join. For example if the +recognition sequence was 'NNNNACGT and the first 4 characters in the +sequence were ACGT, then the match would only be found if the sequence +was +declared as circular. If the sequence is linear then the first fragment +starts at base number 1, and the last ends at the last base. If the +sequence is circular then the length of the first fragment is the +clockwise +distance from the last cut to the first. +.para +Graphical output marks the position of each string by a +short vertical line and gives the name of the enzyme at the left end of +the +line. If the top of the screen is reached the program gives the user the +oportunity to take a hard copy and then will clear the screen and restart +plotting results at the original start position. +.para +Below is an edited piece of dialogue from use of the search option: +.lit +? Menu or option number=17 + +Search for restriction enzyme sites +X 1 Search + 2 List enzyme file + 3 Clear text + 4 Clear graphics +? 0,1,2,3,4 = 2 + + 1 All enzymes +X 2 Six cutters + 3 Four cutters + 4 Personal file + 5 Keyboard +? 0,1,2,3,4,5 = + +AATII/GACGT'C// +ACCI/GT'MKAC// +AFLII/C'TTAAG// +AFLIII/A'CRYGT// +AHAII/GR'CGYC// +APAI/GGGCC'C// +APALI/G'TGCAC// +ASUII/TT'CGAA// +AVAI/C'YCGRG// +AVAII/G'GWCC// +AVRII/C'CTAGG// +BALI/TGG'CCA// +BAMHI/G'GATCC// +BANI/G'GYRCC// +BANII/GRGCY'C// +BBVI/GCAGCNNNNNNNN'/'NNNNNNNNNNNNGCTGC// +BCLI/T'GATCA// +BGLI/GCCNNNN'NGGC// +BGLII/A'GATCT// +BINI/GGATCNNNN'/'NNNNNGATCC// +BSMI/GAATGCN'/NG'CATTC// +BSP1286/GDGCH'C// + +X 1 Search + 2 List enzyme file + 3 Clear text + 4 Clear graphics +? 0,1,2,3,4 = + 1 All enzymes +X 2 Six cutters + 3 Four cutters + 4 Personal file + 5 Keyboard +? 0,1,2,3,4,5 = +? (y/n) (y) Search for all names +X 1 Order results enzyme by enzyme + 2 Order results by position + 3 Show only infrequent cutters + 4 Show names above the sequence +? 0,1,2,3,4 = +? (y/n) (y) List matches +? (y/n) (y) The sequence is linear +? (y/n) (y) Search for definite matches + + Searching + Matches found= 1 + Name Sequence Position Fragment lengths + 1 AATII GACGT'C 112 111 111 + 912 912 + Matches found= 2 + Name Sequence Position Fragment lengths + 1 ACCI GT'CGAC 112 111 111 + 2 ACCI GT'AGAC 420 308 308 + 604 604 + Matches found= 2 + Name Sequence Position Fragment lengths + 1 AHAII GA'CGTC 109 108 90 + 2 AHAII GG'CGTC 199 90 108 + 825 825 + Matches found= 2 + Name Sequence Position Fragment lengths + 1 AVAII G'GACC 84 83 51 + 2 AVAII G'GTCC 973 889 83 + 51 889 + Matches found= 1 + Name Sequence Position Fragment lengths + 1 BALI TGG'CCA 258 257 257 + 766 766 + Matches found= 1 + Name Sequence Position Fragment lengths + 1 BAMHI G'GATCC 92 91 91 + 932 932 + Matches found= 1 + Name Sequence Position Fragment lengths + 1 BANI G'GTGCC 26 25 25 + 998 998 + Matches found= 1 + Name Sequence Position Fragment lengths + 1 BANII GAGCC'C 490 489 489 + 534 534 + Matches found= 11 + Name Sequence Position Fragment lengths + 1 BBVI 'TACTGCGCCGCAGCTGC 38 37 3 + 2 BBVI GCAGCTGCTGGTG' 60 22 22 + 3 BBVI GCAGCGACTGATT' 166 106 28 + 4 BBVI 'CCTGCTAGATTCGCTGC 230 64 37 + 5 BBVI GCAGCGGTACGTA' 452 222 50 + 6 BBVI 'CTCGCCAACGTTGCTGC 502 50 55 + 7 BBVI GCAGCCTTCAACT' 606 104 64 + 8 BBVI 'GAGGTATTCCTGGCTGC 634 28 97 + 9 BBVI 'CTGGCCGCCGCCGCTGC 869 235 104 + 10 BBVI 'GCCGCCGCCGCTGCTGC 872 3 106 + 11 BBVI GCAGCGATGAGGA' 927 55 222 + + ....etc + + X 1 Search + 2 List enzyme file + 3 Clear text + 4 Clear graphics +? 0,1,2,3,4 = + + 1 All enzymes +X 2 Six cutters + 3 Four cutters + 4 Personal file + 5 Keyboard +? 0,1,2,3,4,5 = + +? (y/n) (y) Search for all names + +X 1 Order results enzyme by enzyme + 2 Order results by position + 3 Show only infrequent cutters + 4 Show names above the sequence +? 0,1,2,3,4 = 2 + +? (y/n) (y) List matches +? (y/n) (y) The sequence is linear +? (y/n) (y) Search for definite matches + + Searching + Name Sequence Position Fragment lengths + 1 ECORI G'AATTC 2 1 + 2 BANI G'GTGCC 26 24 + 3 BSP1286 GTGCC'C 31 5 + 4 BBVI 'TACTGCGCCGCAGCTGC 38 7 + 5 NSPBII CAG'CTG 51 13 + 6 PVUII CAG'CTG 51 0 + 7 BBVI GCAGCTGCTGGTG' 60 9 + 8 HINCII GTC'AAC 80 20 + 9 AVAII G'GACC 84 4 + 10 BINI 'CCAGGGATCC 87 3 + 11 BSTNI CC'AGG 89 2 + 12 BAMHI G'GATCC 92 3 + 13 XHOII G'GATCC 92 0 + 14 NSPBII CCG'CTG 98 6 + 15 BINI GGATCCGCT' 100 2 + 16 AHAII GA'CGTC 109 9 + 17 SALI G'TCGAC 111 2 + 18 AATII GACGT'C 112 1 + 19 ACCI GT'CGAC 112 0 + 20 HINCII GTC'GAC 113 1 + + .....etc + +X 1 Search + 2 List enzyme file + 3 Clear text + 4 Clear graphics +? 0,1,2,3,4 = + + 1 All enzymes +X 2 Six cutters + 3 Four cutters + 4 Personal file + 5 Keyboard +? 0,1,2,3,4,5 = + +? (y/n) (y) Search for all names + + 1 Order results enzyme by enzyme +X 2 Order results by position + 3 Show only infrequent cutters + 4 Show names above the sequence +? 0,1,2,3,4 =3 +? Maximum number of cuts (0-100) (0) = +? (y/n) (y) The sequence is linear +? (y/n) (y) Search for definite matches + + Searching + 0 AFLII + 0 AFLIII + 0 APAI + 0 APALI + 0 ASUII + 0 AVAI + 0 AVRII + 0 BCLI + 0 BGLI + 0 BGLII + 0 BSMI + 0 BSPMII + 0 BSTEII + 0 CLAI + 0 DRAI + 0 DRAII + 0 ECOB + 0 ECOK + 0 ECORV + 0 ESPI + + ......etc + +X 1 Search + 2 List enzyme file + 3 Clear text + 4 Clear graphics +? 0,1,2,3,4 = + + 1 All enzymes +X 2 Six cutters + 3 Four cutters + 4 Personal file + 5 Keyboard +? 0,1,2,3,4,5 = + +? (y/n) (y) Search for all names + + 1 Order results enzyme by enzyme + 2 Order results by position +X 3 Show only infrequent cutters + 4 Show names above the sequence +? 0,1,2,3,4 =4 +? (y/n) (y) Hide translation n +? (y/n) (y) Use 1 letter codes +? Line length (30-90) (60) = +? (y/n) (y) The sequence is linear +? (y/n) (y) Search for definite matches + + Searching + ECORI BANI BSP1286 + . . . BBVI NSPBII + . . . . PVUII BBVI +GAATTCGGTTTGGGCTTGGTGTGAGGTGCCCAGAGATTACTGCGCCGCAGCTGCTG +GTGC + 10 20 30 40 50 60 + E F G L G L V * G A Q R L L R R S C W C + N S V W A W C E V P R D Y C A A A A G A + I R F G L G V R C P E I T A P Q L L V L + + HINCII + . AVAII + . . BINI + . . . BSTNI + . . . . BAMHI + . . . . XHOII NSPBII + . . . . . . BINI AHAII + . . . . . . . . SALI + . . . . . . . . .AATII + . . . . . . . . .ACCI + . . . . . . . . ..HINCII +TGGCGGTGCGGAGGTCGTCAACGGACCCAGGGATCCGCTGGACGAGGACGTCGACG +ACGA + 70 80 90 100 110 120 + W R C G G R Q R T Q G S A G R G R R R R + G G A E V V N G P R D P L D E D V D D E + A V R R S S T D P G I R W T R T S T T R + + BBVI BINI +GGAGGAGGTGGATAGCGCATTGCTGGTGGCTGGCAGCGACTGATTTGAGTTCTGAC +CACT + 130 140 150 160 170 180 + G G G G * R I A G G W Q R L I * V L T T + E E V D S A L L V A G S D * F E F * P L + R R W I A H C W W L A A T D L S S D H S + + .......etc + +X 1 Search + 2 List enzyme file + 3 Clear text + 4 Clear graphics +? 0,1,2,3,4 = + + 1 All enzymes +X 2 Six cutters + 3 Four cutters + 4 Personal file + 5 Keyboard +? 0,1,2,3,4,5 =5 +Define search strings by typing a string name +followed by the string(s) +? Name=FRED +? String(s)=AAAAAA/TTTTTT +? Name=MARY +? String(s)=CCCC/GGGG/GCGCT +? Name= +? (y/n) (y) Search for all names +X 1 Order results enzyme by enzyme + 2 Order results by position + 3 Show only infrequent cutters + 4 Show names above the sequence +? 0,1,2,3,4 = +? (y/n) (y) List matches +? (y/n) (y) The sequence is linear +? (y/n) (y) Search for definite matches + Searching + Matches found= 9 + Name Sequence Position Fragment lengths + 1 FRED 'TTTTTT 1557 1556 1 + 2 FRED 'TTTTTT 1558 1 1 + 3 FRED 'TTTTTT 1559 1 1 + 4 FRED 'TTTTTT 1560 1 22 + 5 FRED 'AAAAAA 1582 22 529 + 6 FRED 'AAAAAA 3160 1578 1019 + 7 FRED 'AAAAAA 4204 1044 1044 + 8 FRED 'AAAAAA 5691 1487 1487 + 9 FRED 'AAAAAA 6710 1019 1556 + 529 1578 + Matches found= 36 + Name Sequence Position Fragment lengths + 1 MARY 'CCCC 47 46 1 + 2 MARY 'GGGG 486 439 1 + 3 MARY 'GGGG 487 1 1 + 4 MARY 'CCCC 557 70 1 + 5 MARY 'CCCC 558 1 1 + 6 MARY 'GCGCT 1177 619 1 + + ... etc + +X 1 Search + 2 List enzyme file + 3 Clear text + 4 Clear graphics +? 0,1,2,3,4 = + 1 All enzymes +X 2 Six cutters + 3 Four cutters + 4 Personal file + 5 Keyboard +? 0,1,2,3,4,5 =5 +Define search strings by typing a string name +followed by the string(s) +? Name=JANE +? String(s)=A'TTTT/CC'GGG +? Name= +? (y/n) (y) Search for all names +X 1 Order results enzyme by enzyme + 2 Order results by position + 3 Show only infrequent cutters + 4 Show names above the sequence +? 0,1,2,3,4 = +? (y/n) (y) List matches +? (y/n) (y) The sequence is linear +? (y/n) (y) Search for definite matches + Searching + Matches found= 30 + Name Sequence Position Fragment lengths + 1 JANE A'TTTT 437 436 6 + 2 JANE A'TTTT 546 109 33 + 3 JANE A'TTTT 597 51 43 + 4 JANE A'TTTT 777 180 51 + 5 JANE A'TTTT 1274 497 60 + 6 JANE A'TTTT 1571 297 62 + 7 JANE CC'GGG 1926 355 75 + 8 JANE A'TTTT 2403 477 81 + 9 JANE A'TTTT 2586 183 82 + 10 JANE A'TTTT 2731 145 101 + 11 JANE A'TTTT 2812 81 103 + + ... etc + + +X 1 Search + 2 List enzyme file + 3 Clear text + 4 Clear graphics +? 0,1,2,3,4 =! +.end lit + +.left margin1 +@18. TX 1 7 @ Compare a short sequence +.LEFT MARGIN2 +.para +This routine slides a short sequence along the current sequence and finds +all positions at which a given percentage of the bases match. +Output is in both graphical and listed forms. +.para +If users call for dialogue when the routine is selected they will be given +the choice of keyboard or file input. Define the string, select the "sense" +to use and the percentage match. Matches will be plotted out and then the +user can select to have them listed. Then the routine cycles around. +.para + The routine slides the search string +along the sequence and marks the positions at which a minimum +percentage score is reached. The graphical output draws a vertical line at +the match position; the height of the line represents the percentage +score, +so that if the line reaches the top of the box the score is 100%. +The NC-IUB symbols may be used in the search sequence to encode +uncertain +characters. Any other symbols will not match. +.LIT + + + NC-IUB SYMBOLS + + A,C,G,T + R (A,G) 'puRine' + Y (T,C) 'pYrimidine' + W (A,T) 'Weak' + S (C,G) 'Strong' + M (A,C) 'aMino' + K (G,T) 'Keto' + H (A,T,C) 'not G' + B (G,C,T) 'not A' + V (G,A,C) 'not T' + D (G,A,T) 'not C' + N (G,A,C,T) 'aNy' + + Typical dialogue is shown below. + + +? Menu or option number=18 + Find percentage matches +? (y/n) (y) Keep picture +? String=AAATTTCCC +STRING=AAATTTCCC +? (y/n) (y) This sense +? Percent match (1.00-100.00) (70.00) = + + Missing graphics display here + +Total scoring positions above 70.000 percent = 7 +Scores 7 6 6 6 6 6 6 +Positions 365 212 213 292 311 358 627 +? Display (0-7) (0) =3 + + 365 + ACATTTCGC + * ***** * + AAATTTCCC + 1 + + 212 + GAAACTCCC + ** **** + AAATTTCCC + 1 + + 213 + AAACTCCCA + *** * ** + AAATTTCCC + 1 +? (y/n) (y) Keep picture +Default String=AAATTTCCC +? String= +STRING=AAATTTCCC +? (y/n) (y) This sense n +STRING=GGGAAATTT +? Percent match (1.00-100.00) (70.00) = + + Missing graphics display here + +Total scoring positions above 70.000 percent = 7 +Scores 6 6 6 6 6 6 6 +Positions 269 270 271 288 354 624 853 +? Display (0-7) (0) =3 + + 269 + GAGGGATTT + * * **** + GGGAAATTT + 1 + + 270 + AGGGATTTT + ** * *** + GGGAAATTT + 1 + + 271 + GGGATTTTC + **** ** + GGGAAATTT + 1 +? (y/n) (y) Keep picture ! + +.end lit +.left margin1 +@19. TX 7 @ Compare a short sequence using a score matrix +.LEFT MARGIN2 +.para +This routine slides a short sequence along the current sequence and finds +all positions at which a given level of similarity (a cutoff score) is +reached. The score is defined by use of a score matrix. Output is in both +graphical and listed forms. +.para +If users call for dialogue when the routine is selected they will be given +the choice of keyboard or file input. Define the string, select the "sense" +to use and the cutoff score. Matches will be plotted out and then the user +can select to have them listed. Then the routine cycles around. +.para + The routine slides the search string +along the sequence and marks the positions at which a the cutoff score +is achieved. The graphical output draws a vertical line at +the match position; the height of the line represents the score, +so that if the line reaches the top of the box the score is the maximum +possible. +The NC-IUB symbols may be used in the search sequence to encode +uncertain +characters. +.para + The score matrix reflects the level of +redundancy in the probe sequence and hence will put more emphasis on +those +characters that are better defined. The score matrix is: +.lit + DNA SCORE MATRIX USING IUB SYMBOLS + + T C A G - R Y W S M K H B V D N ? + + T 36 0 0 0 9 0 18 18 0 0 18 12 12 0 12 9 0 + C 0 36 0 0 9 0 18 0 18 18 0 12 12 12 0 9 0 + A 0 0 36 0 9 18 0 18 0 18 0 12 0 12 12 9 0 + G 0 0 0 36 9 18 0 0 18 0 18 0 12 12 12 9 0 + - 9 9 9 9 36 18 18 18 18 18 18 27 27 27 27 36 0 + R 0 0 18 18 18 36 0 9 9 9 9 6 6 12 12 18 0 + Y 18 18 0 0 18 0 36 9 9 9 9 12 12 6 6 18 0 + W 18 0 18 0 18 9 9 36 0 9 9 12 6 6 12 18 0 + S 0 18 0 18 18 9 9 0 36 9 9 6 12 12 6 18 0 + M 0 18 18 0 18 9 9 9 9 36 0 12 6 12 6 18 0 + K 18 0 0 18 18 9 9 9 9 0 36 6 12 6 12 18 0 + H 12 12 12 0 27 6 12 12 6 12 6 36 8 8 8 27 0 + B 12 12 0 12 27 6 12 6 12 6 12 8 36 8 8 27 0 + V 0 12 12 12 27 12 6 6 12 12 6 8 8 36 8 27 0 + D 12 0 12 12 27 12 6 12 6 6 12 8 8 8 36 27 0 + N 9 9 9 9 36 18 18 18 18 18 18 27 27 27 27 36 0 + ? 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + + ? is any unrecognised character. + + Typical dialogue is shown below. + +? Menu or option number=19 + Find matches using a score matrix +? (y/n) (y) Keep picture +? String=AAATTTCCC +STRING=AAATTTCCC +? (y/n) (y) This sense +Minimum score= 0 Maximum score= 324 +? Score (0-324) (280) =250 + + Missing graphics display here + +For score 250 the number of matches= 1 +Scores 252 +Positions 365 +? Display (0-1) (0) =1 + + 365 + ACATTTCGC + * ***** * + AAATTTCCC + 1 +? (y/n) (y) Keep picture +Default String=AAATTTCCC +? String= +STRING=AAATTTCCC +? (y/n) (y) This sense n +STRING=GGGAAATTT +Minimum score= 0 Maximum score= 324 +? Score (0-324) (222) = 200 + + Missing graphics display here + +For score 200 the number of matches= 7 +Scores 216 216 216 216 216 216 216 +Positions 269 270 271 288 354 624 853 +? Display (0-7) (0) =3 + + 269 + GAGGGATTT + * * **** + GGGAAATTT + 1 + + 270 + AGGGATTTT + ** * *** + GGGAAATTT + 1 + + 271 + GGGATTTTC + **** ** + GGGAAATTT + 1 +? (y/n) (y) Keep picture ! + +.end lit +.left margin1 +@20. TX 7 @ Search for a motif using a weight matrix +.LEFT MARGIN2 +.para +This function performs searches for short sequence +motifs using an appropriate weight matrix. In addition it can be used to +create or modify weight matrices. In order to perform a search the only +input +required is the name of the file containing the weight matrix. +The results can be presented graphically or listed. The graphical +presentation will draw line at the position of any matches found; the +height of the line is proportional to the score. +.para +For a search, select "use weight matrix", supply the name of the file +containing the weight matrix, and choose between having results plotted +or listed. If dialogue is requested when the function is selected users can +alter the cutoff score employed. +.para +To create a weight matrix several steps are involved. A file containing an +alignment of known motifs is required. (This file must be created before +the current option is selected. The format is a follows: each sequence is +written on a separate line with at least one space at the beginning; each +sequence is terminated by a space character, and can be followed by a +name. The sequences must be aligned.) Supply the name of the file of +aligned sequences. The program reads and displays the sequences. Choose +between "summing logs of weights" or summing weights (i.e. whether to +multiply or add weights). If logs are used all scores will be negative. +Choose if all positions in the set of aligned sequences should be used or +if a mask should be applied. If so selected, define a mask as a string of +symbols, in which symbol - means ignore and any other symbol means +use. E.g. xx-x--abc means use all positions except 3,5 and 6. +.para +The program will calculate weights as the frequencies of each base at +each unmasked position in the set of aligned sequences. These weights +are then applied to the set of aligned sequences to give a range of +"observed" scores. The mean and standard deviation of these scores is +displayed. The user is asked to supply several values to be used when the +weight matrix is applied to other sequences: a cutoff score (by default, +the mean minus 3 standard deviations), a top score for scaling graphical +results (by default, the mean plus 3 standard deviations), and a position +to identify (this means that if a particular base within the motif is used +as a "landmark", such as the A of the AG in splice acceptor sites, then its +position will be marked in plots). All these values are stored along with +the weight matrix. Finally supply the name of a file to contain the weight +matrix. +.para +Weight matrices can be "rescaled" using a set of aligned sequences in +much the same ways as a matrix is created. The purpose is to redefine +the cutoff scores, and rescaling does not alter any other values in the +weight matrix file. +.para +The methods have changed considerably but were first outlined in +Staden, R. Nucl. Acid Res. 12 505-519 1984, and +Staden, R. Genetic +engineering: principles and methods vol 7, Edited by J.K. Setlow and A. +Hollaender, Plenum publishing corp., 1985. +.para + The methods have always had to deal with the problem of zeroes in the +matrices. The current versions +employ "Laplaces Law of Succession" in which 1 is +added to each term. +.para +It is now possible to apply a mask to a set of aligned sequences in +order to give weight to selected positions only. +Sequences have superimposed functions: some parts may be of general +structural +importance and give rise to an overall framework, and other parts give +specificity and hence are not common; we may want to use a set of +aligned +sequences to define a motif, but want to use only the framework +positions. + Alternatively we may want to pick out +only those parts of a set of aligned sequences that give a particular +property, and to ignore other similarities that are due to some other +property +and which could obscure the pattern +we are interested in. The ability to define a mask allows certain +positions +to be used in the motif and others to be ignored, and yet still permits the +use of a set of aligned sequences to calculate weights. +.para +Typical dialogue is shown below. +.lit + +? Menu or option number=20 +X 1 Use weight matrix + 2 Make weight matrix + 3 Rescale weight matrix +? 0,1,2,3 =2 +? Name of aligned sequences file=[RS.MOTIFS]GCN4.SEQ + + 1 AGCGTGACTCTTCCCGGAA HIS1 + 2 GAGGTGACTCACTTGGAAG HIS1 + 3 CGGATGACTCTTTTTTTTT HIS3 + 4 ACAGTGACTCACGTTTTTT HIS4 + 5 GTCGTGACTCATATGCTTT ARG3 + 6 TGAATGACTCACTTTTTGG ARG4 + 7 TTCTTGACTCGTCTTTTCT CPA1 + 8 CGAATGACTCTTATTGATG CPA2 + 9 AGAATGACTAATTTTACTA TRP5 + 10 TCGTTGACTCATTCTAATC TRP3 + 11 TTGCTGACTCATTACGATT TRP2 + 12 GAGATGACTCTTTTTCTTT IV1 + 13 GCGATGATTCATTTCTCTG IV2 + 14 TAGATGACTCAGTTTAGTC LEU1 + 15 TAAGTGACTCAGTTCTTTC LEU4 + 16 ATGATGACTCTTAAGCATG ILS1 +Length of motif 19 +? (y/n) (y) Sum logs of weights + +? (y/n) (y) Use all motif positions n +x means use, - means ignore +e.g. xx-x---x-x means use positions 1,2,4,8,10 +? Mask=----XXXXXXXX + Applying weights to input sequences + 1 -27.979 AGCGTGACTCTTCCCGGAA + 2 -24.543 GAGGTGACTCACTTGGAAG + 3 -20.890 CGGATGACTCTTTTTTTTT + 4 -23.087 ACAGTGACTCACGTTTTTT + 5 -22.771 GTCGTGACTCATATGCTTT + 6 -23.408 TGAATGACTCACTTTTTGG + 7 -25.159 TTCTTGACTCGTCTTTTCT + 8 -22.679 CGAATGACTCTTATTGATG + 9 -24.751 AGAATGACTAATTTTACTA + 10 -23.157 TCGTTGACTCATTCTAATC + 11 -23.067 TTGCTGACTCATTACGATT + 12 -21.449 GAGATGACTCTTTTTCTTT + 13 -24.191 GCGATGATTCATTTCTCTG + 14 -23.770 TAGATGACTCAGTTTAGTC + 15 -22.923 TAAGTGACTCAGTTCTTTC + 16 -25.285 ATGATGACTCTTAAGCATG +Top score -20.890 Bottom score -27.979 +Mean -23.694 Standard deviation 1.613 +Mean minus 3.sd -28.534 Mean plus 3.sd -18.854 +? Cutoff score (-999.00-9999.00) (-28.53) = +? Top score for scaling plots (-28.53-999.00) (-18.85) = +? Position to identify (0-19) (1) = +? Title=GCN4 SEQUENCES +? Name for new weight matrix file=1.WTS + + +? Menu or option number=20 +X 1 Use weight matrix + 2 Make weight matrix + 3 Rescale weight matrix +? 0,1,2,3 =3 +? Name of existing weight matrix file=1.WTS + GCN4 SEQUENCES +? Name of aligned sequences file=[RS.MOTIFS]GCN4.SEQ +Length of motif 19 +? (y/n) (y) Sum logs of weights n +? (y/n) (y) Use all motif positions + + Applying weights to input sequences + 1 128.000 AGCGTGACTCTTCCCGGAA + 2 148.000 GAGGTGACTCACTTGGAAG + 3 172.000 CGGATGACTCTTTTTTTTT + 4 160.000 ACAGTGACTCACGTTTTTT + 5 161.000 GTCGTGACTCATATGCTTT + 6 157.000 TGAATGACTCACTTTTTGG + 7 149.000 TTCTTGACTCGTCTTTTCT + 8 160.000 CGAATGACTCTTATTGATG + 9 151.000 AGAATGACTAATTTTACTA + 10 159.000 TCGTTGACTCATTCTAATC + 11 158.000 TTGCTGACTCATTACGATT + 12 169.000 GAGATGACTCTTTTTCTTT + 13 152.000 GCGATGATTCATTTCTCTG + 14 157.000 TAGATGACTCAGTTTAGTC + 15 160.000 TAAGTGACTCAGTTCTTTC + 16 143.000 ATGATGACTCTTAAGCATG +Top score 172.000 Bottom score 128.000 +Mean 155.250 Standard deviation 10.034 +Mean minus 3.sd 125.147 Mean plus 3.sd 185.353 +? Cutoff score (-999.00-9999.00) (125.15) = +? Top score for scaling plots (125.15-999.00) (185.35) = +? Position to identify (0-19) (1) = +? Title=GCN4 SEQUENCES +? Name for new weight matrix file=2.WTS + + +? Menu or option number=20 +X 1 Use weight matrix + 2 Make weight matrix + 3 Rescale weight matrix +? 0,1,2,3 = +? Motif weight matrix file=1.WTS + GCN4 SEQUENCES +? (y/n) (y) Plot results n + + 153 -22.61 GCAGCGACTGATTTGAGTT + 169 -28.53 GTTCTGACCACTCAGATCC + 172 -27.27 CTGACCACTCAGATCCGGC + 219 -27.35 CCAGTGGCTGGCCTGCTAG + 268 -27.82 CGAGGGATTTTCGATCTTG + 274 -26.99 ATTTTCGATCTTGTGGATG + 283 -25.79 CTTGTGGATGATTTTCACG + 287 -27.50 TGGATGATTTTCACGTGCG + 298 -28.17 CACGTGCGCCGTCATATTG + 332 -28.27 TCTTTGAAGCAGAAGGGAC + 351 -28.27 AGGGGTACACTTTCACATT + 357 -25.05 ACACTTTCACATTTCGCTT + 364 -28.51 CACATTTCGCTTATGGGAG + 400 -23.77 GAAGTTACTAATGTGCGTG + 451 -26.22 ATGCTCGCCCTCTTTGGTG + 476 -28.00 TCCCTCACTGAGCCCTCCG + 480 -28.33 TCACTGAGCCCTCCGCCTC + 517 -23.46 GCTAAGATTCAGCTTGGTT + 556 -27.27 TCCAGCACTCAGGTTCGGC + 602 -27.01 AACTTGAATCCATCGTTGC + 648 -28.45 TGCTAAACACAGCCGGTTT + 679 -28.18 CTGTTTGCCCAGTTTGGGC + 691 -28.51 TTTGGGCCGCTTCTGGACG + 713 -27.67 GGCTTGACCGTGGCTGTGG + 803 -25.47 ATGCTGACCATGCTTTTCA + 848 -28.11 ATAATGTTAAGTTTGATTC + 857 -25.97 AGTTTGATTCCGCTGGCCG + 879 -27.85 CCGCTGCTGCTGTTTCCAC + 917 -27.77 GCGATGAGGAAGGCTTGTT + 931 -27.81 TTGTTGGCGCGCCTGCTCG + 952 -23.52 GAGGTGACTACCATCCGTG + 977 -28.40 TGCGTGGGTGAGCTGTTGT + + + + +? Menu or option number=6 +Page through text files +? Name of file to read=1.WTS + GCN4 SEQUENCES + 19 1 -28.534 -18.854 + P 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 + N 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 +16 + T 0 0 0 0 16 0 0 1 16 0 5 11 10 12 9 6 7 12 6 + C 0 0 0 0 0 0 0 15 0 15 0 3 2 2 4 3 2 1 3 + A 0 0 0 0 0 0 16 0 0 1 10 0 3 2 0 3 5 2 2 + G 0 0 0 0 0 16 0 0 0 0 1 2 1 0 3 4 2 1 5 +End of file + +.end lit + + +.left margin1 +@21. TX 3 @ Count base composition +.LEFT MARGIN2 +.para +This routine +calculates the base composition of the +active region of the sequence as both totals and percentages. +.left margin1 +@22. TX 3 @ Count dinucleotide frequencies +.LEFT MARGIN2 +.para +This routine simply counts dinucleotide frequencies for the currently +active region of the sequence. It also calculates an expected distribution +based on the base composition. +The output looks like: +.LIT + T C A G + obs expected obs expected obs expected obs expected + + T 8.44 8.25 6.67 7.01 10.35 9.92 3.27 3.54 + C 7.49 7.01 6.76 5.95 8.39 8.43 1.76 3.01 + A 10.13 9.92 7.78 8.43 11.74 11.93 4.89 4.26 + G 2.67 3.54 3.19 3.01 4.06 4.26 2.42 1.52 + +.END LIT +.left margin1 +@23. TX 3 5 @ Count codons and amino acids +.LEFT MARGIN2 +.para +This function +counts codons, amino acid composition, protein molecular weights, and +base +composition. Users select the segments of the sequence that the program +should analyse. +.para +Choose between being shown observed counts or counts normalised so +that the totals for each amino acid sum to 100. Select to define +segments using either the keyboard or an EMBL feature table. +Define the segments to count over. Select strand for each segment. Stop +selecting segments by typing a zero for "Count from ()". The results are +displayed a screenful at a time, and the bell is sounded to show there is +more to come. A zero start position, or the end of an EMBL feature table, +signals +the routine to print out totals for all values. + +.para +The counts are broken down into several figures. + Base +composition by position in codon expressed as a percentage of each bases +own frequency; base composition by position in codon expressed as a +percentage of the overall base composition of the section; base +composition +expected for this amino acid composition if there was no codon +preference; +percentage deviations of the observed amino acid composition from an +average amino acid composition. +.para +The output looks like: +.LIT + + =========================================== + F TTT 1. S TCT 2. Y TAT 2. C TGT 1. + F TTC 1. S TCC 1. Y TAC 3. C TGC 2. + L TTA 7. S TCA 4. * TAA 9. * TGA 1. + L TTG 2. S TCG 1. * TAG 2. W TGG 2. + =========================================== + L CTT 3. P CCT 2. H CAT 4. R CGT 1. + L CTC 2. P CCC 3. H CAC 1. R CGC 0. + L CTA 3. P CCA 2. Q CAA 4. R CGA 0. + L CTG 2. P CCG 2. Q CAG 1. R CGG 2. + =========================================== + I ATT 9. T ACT 1. N AAT 7. S AGT 3. + I ATC 2. T ACC 2. N AAC 4. S AGC 2. + I ATA 4. T ACA 5. K AAA 13. R AGA 5. + M ATG 1. T ACG 2. K AAG 4. R AGG 1. + =========================================== + V GTT 2. A GCT 2. D GAT 1. G GGT 3. + V GTC 2. A GCC 2. D GAC 1. G GGC 1. + V GTA 4. A GCA 3. E GAA 2. G GGA 1. + V GTG 2. A GCG 0. E GAG 1. G GGG 1. + =========================================== + total codons= 166. + T C A G + + 1 31.06 33.68 34.03 35.00 + 2 35.61 35.79 30.89 32.50 + 3 33.33 30.53 35.08 32.50 + + 1 24.70 19.28 39.16 16.87 + 2 28.31 20.48 35.54 15.66 + 3 26.51 17.47 40.36 15.66 + % 26.51 19.08 38.35 16.06 observed, overall totals + % 25.00 22.26 33.10 19.65 expected, even codons per acid + + A C D E F G H I K L + 7. 3. 2. 3. 2. 6. 5. 15. 17. 19. + o-e % -47. -33. -76. -68. -64. -54. 62. 116. 67. 67. + + M N P Q R S T V W Y + 1. 11. 9. 5. 9. 13. 10. 10. 2. 5. + o-e % -62. 66. 12. -17. 19. 21. 6. -2. 0. -5. + total acids= 154. molecular weight= 17421. + + Typical dialogue follows. + +? Menu or option number=23 + Calculate codon usage, base composition + and amino acid composition +? (y/n) (y) Show observed counts +? (y/n) (y) Define segments using keyboard +? Count from (0-1023) (0) =1 +? Count to (1-1023) (1023) =1000 +? (y/n) (y) + strand + + =========================================== + F TTT 13. S TCT 1. Y TAT 1. C TGT 3. + F TTC 4. S TCC 10. Y TAC 1. C TGC 7. + L TTA 1. S TCA 0. * TAA 1. * TGA 4. + L TTG 4. S TCG 1. * TAG 3. W TGG 5. + =========================================== + L CTT 9. P CCT 1. H CAT 3. R CGT 14. + L CTC 7. P CCC 0. H CAC 7. R CGC 14. + L CTA 0. P CCA 0. Q CAA 4. R CGA 9. + L CTG 12. P CCG 1. Q CAG 9. R CGG 8. + =========================================== + I ATT 7. T ACT 4. N AAT 4. S AGT 1. + I ATC 4. T ACC 5. N AAC 3. S AGC 7. + I ATA 1. T ACA 1. K AAA 3. R AGA 2. + M ATG 2. T ACG 1. K AAG 2. R AGG 2. + =========================================== + V GTT 11. A GCT 13. D GAT 6. G GGT 9. + V GTC 5. A GCC 10. D GAC 9. G GGC 11. + V GTA 6. A GCA 5. E GAA 6. G GGA 12. + V GTG 8. A GCG 5. E GAG 3. G GGG 8. + =========================================== + + + Total codons= 333. + T C A G + + 1 23.32 37.69 28.99 40.06 + 2 37.15 22.31 38.46 36.59 + 3 39.53 40.00 32.54 23.34 + ----- ----- ----- ----- + = 100% 100% 100% 100% + + 1 17.72 29.43 14.71 38.14 = 100% + 2 28.23 17.42 19.52 34.83 = 100% + 3 30.03 31.23 16.52 22.22 = 100% + % 25.33 26.03 16.92 31.73 Observed, overall totals + % 24.44 22.31 20.90 32.35 Expected, even codons per acid + + A C D E F G H I K L + 33. 10. 15. 9. 17. 40. 10. 12. 5. 33. +O-E % 22. 81. -13. -55. 34. 71. 40. -29. -73. 13. + + M N P Q R S T V W Y + 2. 7. 2. 13. 49. 20. 11. 30. 5. 2. +O-E % -74. -51. -88. 0. 165. -11. -42. 40. 18. -81. +Total acids= 325. Molecular weight= 35831. Hydrophobicity= -17.8 + + +? Count from (0-1023) (0) = + + Codon totals over all genes + =========================================== + F TTT 13. S TCT 1. Y TAT 1. C TGT 3. + F TTC 4. S TCC 10. Y TAC 1. C TGC 7. + L TTA 1. S TCA 0. * TAA 1. * TGA 4. + L TTG 4. S TCG 1. * TAG 3. W TGG 5. + =========================================== + L CTT 9. P CCT 1. H CAT 3. R CGT 14. + L CTC 7. P CCC 0. H CAC 7. R CGC 14. + L CTA 0. P CCA 0. Q CAA 4. R CGA 9. + L CTG 12. P CCG 1. Q CAG 9. R CGG 8. + =========================================== + I ATT 7. T ACT 4. N AAT 4. S AGT 1. + I ATC 4. T ACC 5. N AAC 3. S AGC 7. + I ATA 1. T ACA 1. K AAA 3. R AGA 2. + M ATG 2. T ACG 1. K AAG 2. R AGG 2. + =========================================== + V GTT 11. A GCT 13. D GAT 6. G GGT 9. + V GTC 5. A GCC 10. D GAC 9. G GGC 11. + V GTA 6. A GCA 5. E GAA 6. G GGA 12. + V GTG 8. A GCG 5. E GAG 3. G GGG 8. + =========================================== + + + Total codons= 333. + T C A G + + 1 23.32 37.69 28.99 40.06 + 2 37.15 22.31 38.46 36.59 + 3 39.53 40.00 32.54 23.34 + ----- ----- ----- ----- + = 100% 100% 100% 100% + + 1 17.72 29.43 14.71 38.14 = 100% + 2 28.23 17.42 19.52 34.83 = 100% + 3 30.03 31.23 16.52 22.22 = 100% + % 25.33 26.03 16.92 31.73 Observed, overall totals + % 24.44 22.31 20.90 32.35 Expected, even codons per acid + + A C D E F G H I K L + 33. 10. 15. 9. 17. 40. 10. 12. 5. 33. +O-E % 22. 81. -13. -55. 34. 71. 40. -29. -73. 13. + + M N P Q R S T V W Y + 2. 7. 2. 13. 49. 20. 11. 30. 5. 2. +O-E % -74. -51. -88. 0. 165. -11. -42. 40. 18. -81. +Total acids= 325. Molecular weight= 35831. Hydrophobicity= -17.8 + +.END LIT +.LEFT MARGIN1 +@24. TX 3 @ Plot base composition +.LEFT MARGIN2 +.para +This option plots the base composition of the sequence. The counts for +any combination of bases can be plotted. +.para +If dialogue is requested the user is presented with a check box for +selecting which bases should be counted, and then allowed to define a +window length, and a "plot interval". Otherwise, the AT composition is +plotted with a window of 101 and a plot interval of 5. +.para +Typical dialogue follows. +.lit +? Menu or option number=d24 + Plot base composition + +checkbox: those set are marked X +X 1 T + 2 C +X 3 A + 4 G +? 0,1,2,3,4 =1 + +checkbox: those set are marked X + 1 T + 2 C +X 3 A + 4 G +? 0,1,2,3,4 =3 + +checkbox: those set are marked X + 1 T + 2 C + 3 A + 4 G +? 0,1,2,3,4 =2 + +checkbox: those set are marked X + 1 T +X 2 C + 3 A + 4 G +? 0,1,2,3,4 =4 + +checkbox: those set are marked X + 1 T +X 2 C + 3 A +X 4 G +? 0,1,2,3,4 = + +? odd span length (1-201) (31) = +? plot interval (1-11) (5) = + + missing graphics + + + +.end lit +.left margIN1 +@25. TX 3 @ Plot local deviations in base composition +.LEFT MARGIN2 +.para +The "local deviation" routines are designed to indicate the similarity of +the compositions of different parts of the sequence. The composition of +every segment of the sequence is compared with a standard composition. +The levels of similarity are plotted as a chi squared values. The standard +can be the composition of the whole sequence, or alternatively that of a +small segment defined by the user. +.para +If dialogue is forced define the standard region, the window length and +the plot interval. Otherwise the composition of the whole sequence is +taken as a standard. The maximum and minimum observed value of the chi +squared calculation is displayed, and plots will always exactly fill the +available box. Any unusual regions will show as peaks. +.para +The following measure is used: for each window position +calculate (sum((obs-exp)*(obs-exp))/(exp*exp)) +where obs is the observed composition +and exp is the expected composition (the composition of the standard). + The calculation is performed once to find out the range of values and is +then repeated and +plotted so that the plot exactly fills the allocated screen space. +.left margIN1 +@26. TX 3 @ Plot local deviations from dinucleotide composition +.LEFT MARGIN2 +.para +The "local deviation" routines are designed to indicate the similarity of +the compositions of different parts of the sequence. The dinucleotide +composition of every segment of the sequence is compared with a +standard composition. The levels of similarity are plotted as a chi +squared values. The standard can be the composition of the whole +sequence, or alternatively that of a small segment defined by the user. +.para +If dialogue is forced define the standard region, the window length and +the plot interval. Otherwise the composition of the whole sequence is +taken as a standard. The maximum and minimum observed value of the chi +squared calculation is displayed, and plots will always exactly fill the +available box. Any unusual regions will show as peaks. +.para +The following measure is used: for each window position +calculate (sum((obs-exp)*(obs-exp))/(exp*exp)) +where obs is the observed composition +and exp is the expected composition (the composition of the standard). + The calculation is performed once to find out the range of values and is +then repeated and +plotted so that the plot exactly fills the allocated screen space. +.left margin1 +@27. TX 3 @ Plot local deviations from trinucleotide composition +.LEFT MARGIN2 +.para +The "local deviation" routines are designed to indicate the similarity of +the compositions of different parts of the sequence. The trinucleotide +composition of every segment of the sequence is compared with a +standard composition. The levels of similarity are plotted as a chi +squared values. The standard can be the composition of the whole +sequence, or alternatively that of a small segment defined by the user. +.para +If dialogue is forced define the standard region, the window length and +the plot interval. Otherwise the composition of the whole sequence is +taken as a standard. The maximum and minimum observed value of the chi +squared calculation is displayed, and plots will always exactly fill the +available box. Any unusual regions will show as peaks. +.para +The following measure is used: for each window position +calculate (sum((obs-exp)*(obs-exp))/(exp*exp)) +where obs is the observed composition +and exp is the expected composition (the composition of the standard). + The calculation is performed once to find out the range of values and is +then repeated and +plotted so that the plot exactly fills the allocated screen space. +.left margin1 +@28. TX 5 @ Calculate codon constraint +.left margin2 +.para +The purpose of this option (which is somewhat specialised) is to measure +the level of constraint imposed on the sequence by coding for a protein of +the observed composition. It measures the strength of the codon bias +averaged over windows of 99 codons and displays the values observed. +.para +Select between defining segments at the keyboard or using an EMBL +feature table. Finish selecting segments by typing a zero start. The value +for each segment is displayed: +.para + Mean (W-EW) / EWD, window 99 10.5 +.para +The codon constraint is the +difference between the observed codon improbability and the mean +improbabilty for +a sequence of the same composition. See McLachlan, Staden and Boswell +Nucl. Acid Res. 1984 + +.left margin1 +@59. TX 3 @ Plot negentropy +.LEFT MARGIN2 +.para +This routine is designed to show regions of the sequence that differ in +composition from others, and hence is like the "plot deviation.." routines. +.para +Negentropy or information is defined in the following way: let Pi be the +probability of observing base i, where i = A,C,G or T, then the average +information per base is +I=-sum(Pi.Log(Pi)) (sum over all i). This routine calculates Pi by +calculating the overall composition for the sequence and then plots I for +windows of length defined by the user. +.left margin1 +@30. TX 4 @ Search for hairpin loops +.LEFT MARGIN2 +.para +Used to find simple inverted repeats or potential hairpin loops + The loops are defined by a range of sizes for +the loop and a minimum number of consecutive base pairs in the stem. +The results can be presented graphically or listed. +A-T, G-C and G-T basepairs are counted. +.para +Define the range of loop sizes and the minimum number of consecutive +basepairs required. Choose between plotted or listed results. +.para +The loops found are plotted as blips on a +horizontal line that represents the sequence, the heights of the lines are +proportional to the number of basepairs in the stems. Note that only +uninterrupted stems are found - i.e. all basepairs must be made. To look +for stems with some unpaired bases (or for palindromes) use the inverted +repeat motif class in the pattern searching option. +.para +Typical dialogue follows. +.lit +? Menu or option number=30 + Search for hairpin loops +Define the range of loop sizes +? Minimum loop size (1-30) (1) = +? Maximum loop size (3-60) (3) = +? Minimum number of basepairs (2-20) (6) = +? (y/n) (y) Plot results n + Searching + + T.G + G-C + G.T + T.G + C-G + G-C + T.G + C-G + G.T + GCCGCA GCGGAGG + 49 + + G + G-C + T.G + C-G + G.T + T.G + G-C + CTGCTG GGAGGTC + 56 + + + G + T.G + G-C + G.T + T.G + C-G + G-C + T-A + T.G + AGCGCA CGACTGA + 139 + + A C + G.T + C-G + G.T + C-G + C-G + G-C + TTCGCT CAACGCC + 244 + +.end lit +.LEFT MARGIN1 +@31. TX 4 @ Search for long range inverted repeats +.LEFT MARGIN2 +.para +Searches for inverted repeats. The repeats found are exact matches of at +least 6 consecutive bases. Results can be presented graphically or listed. +Plotted results show the end points of repeats joined by rectangular +lines. +.para +If dialogue is not requested the defaults will be taken. Otherwise choose +between plotted or listed results. If required select to analyse a +restricted segment of the currently active region. Choose a repeat length. +.para +Typical dialogue follows. +.lit +? Menu or option number=D31 + Plot long-range inverted repeats +? (y/n) (y) Plot results n +Define restricted region +? start (1-1023) (1) = +? end (2-1023) (1023) = +? Minimum inverted repeat (6-30) (12) =10 + Searching + 27 909 10 TGCCCAGAGA + +.end lit +.LEFT MARGIN1 +@32. TX 4 @ Search for repeats +.LEFT MARGIN2 +.para +Searches for direct repeats. The repeats found are exact matches of at +least 6 consecutive bases. Results can be presented graphically or listed. +Plotted results show the end points of repeats joined by rectangular +lines. +.para +If dialogue is not requested the defaults will be taken. Otherwise choose +between plotted or listed results. If required select to analyse a +restricted segment of the currently active region. Choose a repeat length. +.para +Typical dialogue follows. + +.lit + ? Menu or option number=D32 + Plot repeats +? (y/n) (y) Plot results n +Define restricted region +? start (1-1023) (1) = +? end (2-1023) (1023) = +? Minimum repeat (6-30) (12) =8 + Searching + 619 988 8 GCTGTTGT + 514 646 8 GCTGCTAA + 94 865 8 TCCGCTGG + 146 222 9 GTGGCTGGC + 455 497 8 TCGCCCTC + 454 496 9 CTCGCCCTC + 872 875 8 GCCGCCGC + 510 615 8 CGTTGCTG + 152 913 8 GGCAGCGA + 199 265 8 CGTCGAGG + 689 794 8 AGTTTGGG + 147 223 8 TGGCTGGC + 101 116 8 GACGAGGA + 8 690 8 GTTTGGGC + 52 141 8 TGCTGGTG + +.end lit +.left margin1 +@33. TX 4 @ Search for z dna (total ry, yr) +.LEFT MARGIN2 +.para +Searches for segments of the sequence that might form Z DNA. A window +length is chosen and the number of RY and YR dinucleotides within each +window is plotted. The top of the box corresponds to all RY or YR, the +bottom to zero RY or YR. +.para +If dialogue is requested, select a window length and plot interval. +Otherwise the defaults will be used. +.para +The program contains three +separate ways of doing this (options 33,34,35). +.left margin1 +@34. TX 4 @ Search for z dna (runs of ry, yr) +.LEFT MARGIN2 +.para +Searches for segments of the sequence that might form Z DNA. Results +are plotted. +.para +If dialogue is requested define a window length and plot interval. +Otherwise the defaults will be used. + The routine +counts the number of R in positions 1,3,5 etc =R1, the number of Y in +positions 2,4,6 etc =Y1, the number of Y in positions 1,3,5 etc =Y2 and +the +number of R in positions 2,4,6 etc =R2 for a window length. It plots the +maximum of R1+Y1 and R2+Y2 relative to a minimum of (window +length)/2 and a +maximum of (window length). (see 33,35). +.LEFT MARGIN1 +@35. TX 4 @ Search for z dna (best phased value) +.LEFT MARGIN2 +.para +Searches for segments of the sequence that might form Z DNA. Results +are plotted. +.para +If dialogue is requested define a window length and a plot interval. +Ohterwise the defaults values will be used. +.para + The routine +counts the number of consecutive RY or YR dinucleotides in phase. It +moves +through the sequence counting the number of RY or YR dinucleotides; when +the next dinucleotide is not of the correct type the score is set back to +zero and the search restarted using the current base to set the phase. The +plots are done relative to a minimum of zero and a maximum defined by +the +user. (See 33,34). +.LEFT MARGIN1 +@36. TX 4 @ Local similarity or complementarity search +.LEFT MARGIN2 +.PARA +This function is designed to find segments of +local similarity or complementarity. It is therefore like performing +a DIAGON +plot that is +restricted to regions near the main diagonal. Results can be presented +graphically or listed. +.para +Users define +a region to search through, +a span length, a range for searching through and a cut-off score. The +program takes all sections of sequence +of length span within the defined region + and compares them to +all other sequences within the region and +range specified. +If a match above the cutoff is found we +need to show the position +of the two sections of sequence and the score, and we do it in the +following way. +If we have a 70% +match between +a sequence that starts at p1 and a sequence that starts at p2 +the program draws a +diagonal line that starts at p1 with height 70% of the box and which +finishes at p2 with +height 0. +The matches can also be listed. +.para +Here I define the terms range, region, and span and what is compared. +Suppose we have a defined region j1 to j2, a range of i1 to i2 and a span +of +s; the program will take, in turn, all sections of sequence of length s +within j1 and j2 and compare them to all sequences that start a distance +i1+s-1 +to i2+s-1 away from them. First it will take the sequence of length s +starting +at j1 and compare it +with the sequence of length s starting at +j1+s-1+i1, then j1+s-1+i1+1, etc up to j1+s-1+i2; then it will take the +sequence of length s starting at j1+1 and compare it with the sequence +starting at j1+s-1+1+i1 etc. This continues until we hit + the right hand end of the +sequence as defined by j2. Note 1)that sequences are not compared with +themselves: the nearest sequence compared to a span s starting at j +starts +at j+s; 2) ranges i1 and i2 are ranges of start positions; 3) by choosing a +range greater than the length of the sequence this routine will do a full +DIAGON analysis except for those points within a distance span of + the main diagonal (see note 1). +.para +Typical dialog follows. +.lit + +? Menu or option number=36 + Search for local similarity or complementarity +? (y/n) (y) Find direct repeats +? (y/n) (y) Keep picture n +? Span (5-200) (15) = +Define restricted region +? start (0-1023) (1) = +? end (2-1023) (1023) = +? Percent match (1.00-100.00) (70.00) = +? Range start (1-50) (1) = +? Range end (1-50) (1) =5 +? (y/n) (y) Plot results n + Working + + + 118 128 + CGAGGAGGAG GTGGA + ** ***** ** ** + GGACGAGGAC GTCGA + 100 110 + + + 119 129 + GAGGAGGAGG TGGAT + ** ***** * * ** + GACGAGGACG TCGAC + 101 111 +? (y/n) (y) Find direct repeats n +? (y/n) (y) Keep picture +? Span (5-200) (15) = +Define restricted region +? start (0-1023) (1) = +? end (2-1023) (1023) = +? Percent match (1.00-100.00) (70.00) = +? Range start (1-50) (1) = +? Range end (1-50) (5) =8 +? (y/n) (y) List results + + Working + + + 178 188 + ACTCAGATCC GGCGG + ***** *** * ** + ACTCAAATCA GTCGC + 156 166 + + + 177 187 + CACTCAGATC CGGCG + ***** *** * ** + AACTCAAATC AGTCG + 157 167 +? (y/n) (y) Find inverted repeats ! +.end lit + +.left margin1 +@37. TX 5 @ Set genetic code +.LEFT MARGIN2 +.para +This function allows the user to change the current active genetic code +for +all the options. The user may select: the standard code, the mammalian +mitochondrial code, the yeast mitochondrial code or a personal code +(define +your own). +.para +Select code. If personal, define a codon and select an amino acid. When all +codons have been reset define a blank codon. +.para +The code differences are: +.lit + Mammalian Yeast + Codon Mitochondrial Mitochondrial Standard + UGA W W STOP + AUA M M I + CUA L T L + AGA STOP R R + AGG STOP R R +.END LIT +.para +Typical dialogue follows. + +.lit +? Menu or option number=37 +X 1 Standard code + 2 Mammalian mitochondrial code + 3 Yeast mitochondrial code + 4 Personal code +? 0,1,2,3,4 =2 + +? Menu or option number=37 +X 1 Standard code + 2 Mammalian mitochondrial code + 3 Yeast mitochondrial code + 4 Personal code +? 0,1,2,3,4 =4 +Define genetic code by typing a codon +followed by a 1 letter amino acid symbol +? Codon=TTT +Default Amino acid symbol=F +? Amino acid symbol=W +? Codon= +.end lit + +.left margin1 +@38. T 3 4 @ Examine repeats +.left margin2 +.para +This function can be used to examine the frequencies of repeated words +within a sequence. It finds all words that occur more than once. The +user selects a minimum word length and the program finds all words of that +length that occur more than once; then it "follows" each repeated word until it +becomes unique. For each word length it can report the number of different +repeated words, the number of occurrences of each word, and their actual +positions and sequences. +.para +It is possible that the algorithm may run out of memory, paticularly if a short +mimimum word length is chosen, or if the sequence is very long or very +repetitive. If this occurs the longest reported word length will not +necessarily be the longest in the sequence: the memory will have been consumed +before the longest word is found. +.lit +Typical dialogue and output is shown below. + + Expected length of longest repeat 14 + ? Minumim word length (1-6) (6) =6 + Working + ? Show repeat frequencies for words of at least length (6-15) (15) =10 + For length 10 the number of different repeated words is 2035 + For length 11 the number of different repeated words is 613 + For length 12 the number of different repeated words is 161 + For length 13 the number of different repeated words is 37 + For length 14 the number of different repeated words is 10 + For length 15 the number of different repeated words is 1 + ? Show repeats for words of length (6-15) (15) =14 + ? Show repeats for words occuring with frequency (2-9999) (2) =2 + + ggtgctcatgccca + occurs at 21611 + occurs at 21851 + ttatccggtgatga + occurs at 4604 + occurs at 8806 + agcaccacgctgac + occurs at 5954 + occurs at 9486 + catgacggaggatg + occurs at 10480 + occurs at 19925 + aaagacgggaaaat + occurs at 11820 + occurs at 43157 + tacaaaaccaattt + occurs at 26797 + occurs at 31369 + cgagaaagagtgcg + occurs at 4260 + occurs at 44305 + gccggatgatggcg + occurs at 7893 + occurs at 16638 + atgacggaggatga + occurs at 10481 + occurs at 19926 + gcggcgaacgaggc + occurs at 11352 + occurs at 18718 + ? Show repeats for words of length (6-15) (15) =! + +Example of not enough memory +---------------------------- + + Expected length of longest repeat 14 + ? Minumim word length (1-6) (6) =1 + Working + Not enough memory + Memory used in bytes 1125996. Length of longest repeat 5 + ? Show repeat frequencies for words of at least length (1-5) (5) =! + +.end lit +.left margin1 +@39. TX 5 @ Translate and list in upto six phases +.LEFT MARGIN2 +.para +This is a general listing function that will perform translations and +produce several forms of output. The possibilities are: +.lit +1) no translation, list one or two strands, two ways of numbering the +sequence. +2) translation, one or two strands, one or three letter codes. + Positions defined by: + a) open reading frames of some minimum length l, l can be 0, hence giving +a complete six phase translation. + b) positions typed on keyboard, again 1 to 6 phases, translations appearing +above and below the dna. + c) positions read from a feature table. + +It should be used in preference to option 5. For publication +without a translation, the option to number ends of lines is more compact +than option 5. Some examples and typical dialogue are given below. Note the +requirement for d39. + +? Menu or option number=D39 +Find open reading frames, translate and list +? (y/n) (y) Show translation + +The segments to translate can be + 1 Typed on the keyboard + 2 Read from a feature table +X 3 Open reading frames +? 1,2,3 = +? Minimum open frame in amino acids (0-7238) (30) = +? (y/n) (y) Use 1 letter codes +Define section of DNA to display +? start (1-7238) (1) = +? end (2-7238) (7238) =300 +? Line length (30-120) (60) = +Which strands should be shown +X 1 + strand only + 2 - strand only + 3 Both strands +? 1,2,3 =3 +? (y/n) (y) Number ends of lines + + + N A T T I S R I D A T F S A R A P N E N + AACGCTACTACTATTAGTAGAATTGATGCCACCTTTTCAGCTCGCGCCCCAAATGAAAAT 60 + . : . : . : . : . : . : + TTGCGATGATGATAATCATCTTAACTACGGTGGAAAAGTCGAGCGCGGGGTTTACTTTTA + * S A G W I F I + A V V I L L I S A V K E A R A G F S F + + I A K Q V I D H L R N V S N G Q T K S T + L N R L L T I C E M Y L M V K L N L L + ATAGCTAAACAGGTTATTGACCATTTGCGAAATGTATCTAATGGTCAAACTAAATCTACT 120 + . : . : . : . : . : . : + TATCGATTTGTCCAATAACTGGTAAACGCTTTACATAGATTACCAGTTTGATTTAGATGA + Y S F L N N V M Q S I Y R I T L S F R S + I A L C T I S W K R F T D L P * V L D V + + R S Q N W E S T V T W N E T S R H R T L + V R R I G N Q L L H G M K L P D T V L * + CGTTCGCAGAATTGGGAATCAACTGTTACATGGAATGAAACTTCCAGACACCGTACTTTA 180 + . : . : . : . : . : . : + GCAAGCGTCTTAACCCTTAGTTGACAATGTACCTTACTTTGAAGGTCTGTGGCATGAAAT + T R L I P F + R E C F Q S D V T V H F S V E L C R V K + + V A Y L K H V E L Q H Q I Q Q L S S K P + GTTGCATATTTAAAACATGTTGAGCTACAGCACCAGATTCAGCAATTAAGCTCTAAGCCA 240 + . : . : . : . : . : . : + CAACGTATAAATTTTGTACAACTCGATGTCGTGGTCTAAGTCGTTAATTCGAGATTCGGT + T A Y K F C T S S C C W I + + S A K M T S Y Q K E Q L K V L S N P D L + TCCGCAAAAATGACCTCTTATCAAAAGGAGCAATTAAAGGTACTCTCTAATCCTGACCTG 300 + . : . : . : . : . : . : + AGGCGTTTTTACTGGAGAATAGTTTTCCTCGTTAATTTCCATGAGAGATTAGGACTGGAC + + +? Menu or option number=D39 +Find open reading frames, translate and list +? (y/n) (y) Show translation N +Define section of DNA to display +? start (1-7238) (1) = +? end (2-7238) (7238) =300 +? Line length (30-120) (60) = +Which strands should be shown +X 1 + strand only + 2 - strand only + 3 Both strands +? 1,2,3 = +? (y/n) (y) Number ends of lines + + + AACGCTACTACTATTAGTAGAATTGATGCCACCTTTTCAGCTCGCGCCCCAAATGAAAAT 60 + + ATAGCTAAACAGGTTATTGACCATTTGCGAAATGTATCTAATGGTCAAACTAAATCTACT 120 + + CGTTCGCAGAATTGGGAATCAACTGTTACATGGAATGAAACTTCCAGACACCGTACTTTA 180 + + GTTGCATATTTAAAACATGTTGAGCTACAGCACCAGATTCAGCAATTAAGCTCTAAGCCA 240 + + TCCGCAAAAATGACCTCTTATCAAAAGGAGCAATTAAAGGTACTCTCTAATCCTGACCTG 300 + + +? Menu or option number=D39 +Find open reading frames, translate and list +? (y/n) (y) Show translation +The segments to translate can be + 1 Typed on the keyboard + 2 Read from a feature table +X 3 Open reading frames +? 1,2,3 = +? Minimum open frame in amino acids (0-7238) (30) =0 +? (y/n) (y) Use 1 letter codes N +Define section of DNA to display +? start (1-7238) (1) = +? end (2-7238) (7238) =300 +? Line length (30-120) (60) = +Which strands should be shown +X 1 + strand only + 2 - strand only + 3 Both strands +? 1,2,3 =3 +? (y/n) (y) Number ends of lines + + + AsnAlaThrThrIleSerArgIleAspAlaThrPheSerAlaArgAlaProAsnGluAsn + ThrLeuLeuLeuLeuValGluLeuMetProProPheGlnLeuAlaProGlnMetLysIle + ArgTyrTyrTyr******Asn***CysHisLeuPheSerSerArgProLys***Lys + AACGCTACTACTATTAGTAGAATTGATGCCACCTTTTCAGCTCGCGCCCCAAATGAAAAT 60 + . : . : . : . : . : . : + TTGCGATGATGATAATCATCTTAACTACGGTGGAAAAGTCGAGCGCGGGGTTTACTTTTA + ValSerSerSerAsnThrSerAsnIleGlyGlyLys***SerAlaGlyTrpIlePheIle + Arg************TyrPheGlnHisTrpArgLysLeuGluArgGlyLeuHisPheTyr + AlaValValIleLeuLeuIleSerAlaValLysGluAlaArgAlaGlyPheSerPhe + + IleAlaLysGlnValIleAspHisLeuArgAsnValSerAsnGlyGlnThrLysSerThr + ***LeuAsnArgLeuLeuThrIleCysGluMetTyrLeuMetValLysLeuAsnLeuLeu + TyrSer***ThrGlyTyr***ProPheAlaLysCysIle***TrpSerAsn***IleTyr + ATAGCTAAACAGGTTATTGACCATTTGCGAAATGTATCTAATGGTCAAACTAAATCTACT 120 + . : . : . : . : . : . : + TATCGATTTGTCCAATAACTGGTAAACGCTTTACATAGATTACCAGTTTGATTTAGATGA + TyrSerPheLeuAsnAsnValMetGlnSerIleTyrArgIleThrLeuSerPheArgSer + Leu***ValPro***GlnGlyAsnAlaPheHisIle***HisAspPhe***Ile***Glu + IleAlaLeuCysThrIleSerTrpLysArgPheThrAspLeuPro***ValLeuAspVal + + ArgSerGlnAsnTrpGluSerThrValThrTrpAsnGluThrSerArgHisArgThrLeu + ValArgArgIleGlyAsnGlnLeuLeuHisGlyMetLysLeuProAspThrValLeu*** + SerPheAlaGluLeuGlyIleAsnCysTyrMetGlu***AsnPheGlnThrProTyrPhe + CGTTCGCAGAATTGGGAATCAACTGTTACATGGAATGAAACTTCCAGACACCGTACTTTA 180 + . : . : . : . : . : . : + GCAAGCGTCTTAACCCTTAGTTGACAATGTACCTTACTTTGAAGGTCTGTGGCATGAAAT + ThrArgLeuIleProPhe***SerAsnCysProIlePheSerGlySerValThrSer*** + AsnAlaSerAsnProIleLeuGln***MetSerHisPheLysTrpValGlyTyrLysLeu + ArgGluCysPheGlnSerAspValThrValHisPheSerValGluLeuCysArgValLys + + ValAlaTyrLeuLysHisValGluLeuGlnHisGlnIleGlnGlnLeuSerSerLysPro + LeuHisIle***AsnMetLeuSerTyrSerThrArgPheSerAsn***AlaLeuSerHis + SerCysIlePheLysThrCys***AlaThrAlaProAspSerAlaIleLysLeu***Ala + GTTGCATATTTAAAACATGTTGAGCTACAGCACCAGATTCAGCAATTAAGCTCTAAGCCA 240 + . : . : . : . : . : . : + CAACGTATAAATTTTGTACAACTCGATGTCGTGGTCTAAGTCGTTAATTCGAGATTCGGT + AsnCysIle***PheMetAsnLeu***LeuValLeuAsnLeuLeu***AlaArgLeuTrp + GlnMetAsnLeuValHisGlnAlaValAlaGlySerGluAlaIleLeuSer***AlaMet + ThrAlaTyrLysPheCysThrSerSerCysCysTrpIle***CysAsnLeuGluLeuGly + + SerAlaLysMetThrSerTyrGlnLysGluGlnLeuLysValLeuSerAsnProAspLeu + ProGlnLys***ProLeuIleLysArgSerAsn***ArgTyrSerLeuIleLeuThrCys + IleArgLysAsnAspLeuLeuSerLysGlyAlaIleLysGlyThrLeu***Ser***Pro + TCCGCAAAAATGACCTCTTATCAAAAGGAGCAATTAAAGGTACTCTCTAATCCTGACCTG 300 + . : . : . : . : . : . : + AGGCGTTTTTACTGGAGAATAGTTTTCCTCGTTAATTTCCATGAGAGATTAGGACTGGAC + GlyCysPheHisGlyArgIleLeuLeuLeuLeu***LeuTyrGluArgIleArgValGln + ArgLeuPheSerArgLysAspPheProAlaIleLeuProValArg***AspGlnGlyThr + AspAlaPheIleValGlu******PheSerCysAsnPheThrSerGluLeuGlySerArg + + +? Menu or option number=D39 +Find open reading frames, translate and list +? (y/n) (y) Show translation +The segments to translate can be + 1 Typed on the keyboard + 2 Read from a feature table +X 3 Open reading frames +? 1,2,3 =1 +? (y/n) (y) Use 1 letter codes +Define section of DNA to display +? start (1-7238) (1) = +? end (2-7238) (7238) =300 +? Line length (30-120) (60) = +Which strands should be shown +X 1 + strand only + 2 - strand only + 3 Both strands +? 1,2,3 = +? (y/n) (y) Number ends of lines N +Translate +? From (0-300) (0) =101 +? To (1-300) (300) =300 +Translate +? From (0-300) (0) =102 +? To (1-300) (300) =200 +Translate +? From (0-300) (0) = + + + AACGCTACTACTATTAGTAGAATTGATGCCACCTTTTCAGCTCGCGCCCCAAATGAAAAT + 10 20 30 40 50 60 + + M V K L N L L + W S N * I Y + ATAGCTAAACAGGTTATTGACCATTTGCGAAATGTATCTAATGGTCAAACTAAATCTACT + 70 80 90 100 110 120 + + V R R I G N Q L L H G M K L P D T V L * + S F A E L G I N C Y M E * N F Q T P Y F + CGTTCGCAGAATTGGGAATCAACTGTTACATGGAATGAAACTTCCAGACACCGTACTTTA + 130 140 150 160 170 180 + + L H I * N M L S Y S T R F S N * A L S H + S C I F K T C + GTTGCATATTTAAAACATGTTGAGCTACAGCACCAGATTCAGCAATTAAGCTCTAAGCCA + 190 200 210 220 230 240 + + P Q K * P L I K R S N * R Y S L I L T C + TCCGCAAAAATGACCTCTTATCAAAAGGAGCAATTAAAGGTACTCTCTAATCCTGACCTG + 250 260 270 280 290 300 + + +? Menu or option number=D39 +Find open reading frames, translate and list +? (y/n) (y) Show translation +The segments to translate can be + 1 Typed on the keyboard + 2 Read from a feature table +X 3 Open reading frames +? 1,2,3 =2 +? Embl feature table file=1.FT +? (y/n) (y) Use 1 letter codes +Define section of DNA to display +? start (1-7238) (1) = +? end (2-7238) (7238) =300 +? Line length (30-120) (60) = +Which strands should be shown +X 1 + strand only + 2 - strand only + 3 Both strands +? 1,2,3 =3 +? (y/n) (y) Number ends of lines + + + N A T T I S R I D A T F S A R A P N E N + AACGCTACTACTATTAGTAGAATTGATGCCACCTTTTCAGCTCGCGCCCCAAATGAAAAT 60 + . : . : . : . : . : . : + TTGCGATGATGATAATCATCTTAACTACGGTGGAAAAGTCGAGCGCGGGGTTTACTTTTA + * S A G W I F I + A V V I L L I S A V K E A R A G F S F + + I A K Q V I D H L R N V S N G Q T K S T + L N R L L T I C E M Y L M V K L N L L + ATAGCTAAACAGGTTATTGACCATTTGCGAAATGTATCTAATGGTCAAACTAAATCTACT 120 + . : . : . : . : . : . : + TATCGATTTGTCCAATAACTGGTAAACGCTTTACATAGATTACCAGTTTGATTTAGATGA + Y S F L N N V M Q S I Y R I T L S F R S + I A L C T I S W K R F T D L P * V L D V + + R S Q N W E S T V T W N E T S R H R T L + V R R I G N Q L L H G M K L P D T V L * + CGTTCGCAGAATTGGGAATCAACTGTTACATGGAATGAAACTTCCAGACACCGTACTTTA 180 + . : . : . : . : . : . : + GCAAGCGTCTTAACCCTTAGTTGACAATGTACCTTACTTTGAAGGTCTGTGGCATGAAAT + T R L I P F + R E C F Q S D V T V H F S V E L C R V K + + V A Y L K H V E L Q H Q I Q Q L S S K P + GTTGCATATTTAAAACATGTTGAGCTACAGCACCAGATTCAGCAATTAAGCTCTAAGCCA 240 + . : . : . : . : . : . : + CAACGTATAAATTTTGTACAACTCGATGTCGTGGTCTAAGTCGTTAATTCGAGATTCGGT + T A Y K F C T S S C C W I + + S A K M T S Y Q K E Q L K V L S N P D L + TCCGCAAAAATGACCTCTTATCAAAAGGAGCAATTAAAGGTACTCTCTAATCCTGACCTG 300 + . : . : . : . : . : . : + AGGCGTTTTTACTGGAGAATAGTTTTCCTCGTTAATTTCCATGAGAGATTAGGACTGGAC + * L Y E R I R V Q + * F S C N F T S E L G S R +.end lit +.left margin1 +@40. TX 5 @ Translate and write the protein sequence to disk +.LEFT MARGIN2 +.para +This routine allows the user to translate sections of the sequence into +the +1 letter amino acid codes and store the resulting amino acid sequences in +a disk file. +Two modes of use are possible. Either all open reading frames of at least +some minimum length will +automatically be found and translated, or the user can specify that +particular segments be translated. +.para +Mode 1: the user selects to to translate all open reading frames. +.para +Either, or both, strands can be +translated. + The output file is in the same format as a PIR .seq file. +Each protein segment is given an entry name that is its start base in +the DNA, and a title that includes its end position, +reading frame and strand (+ for plus, - for minus). +Each segment is terminated by * whether or not +there is a stop codon in the DNA. The file is therefore suitable for input +to FASTA, ALIGNL and ANALYSEPL. +.para +Mode 2: the user selects to identify the segments to translate. +.para +Either, or both, strands can be +translated. +If multiple coding regions +are translated each will be separated from the previous one by a gap of 5 +dashes (-----). +The sections to translate can be +defined from the keyboard or by supplying the name of the appropriate +EMBL +library feature table. +.para +Typical dialogue follows. +.lit +? Menu or option number=40 + Translate and write protein sequence to disk +? (y/n) (y) Translate selected regions +? (y/n) (y) Define segments using keyboard +Translate +? From (0-1023) (0) =1 +? To (1-1023) (1023) =111 +? (y/n) (y) + strand +Translate +? From (0-1023) (0) = +? Output file name=1.OUT + + ? Menu or option number=40 + Translate and write protein sequence to disk +? (y/n) (y) Translate selected regions n +? Minimum open frame in amino acids (5-1000) (30) = + +X 1 + strand only + 2 - strand only + 3 Both strands +? 0,1,2,3 =3 +? File name for translation=1.OUT + +? Menu or option number=6 +Page through text files +? Name of file to read=1.OUT +>P1; 25 + 135 1 + + GAQRLLRRSCWCWRCGGRQRTQGSAGRGRRRRGGGG* +>P1; 238 + 486 1 + + IRCRDCGQRRRGIFDLVDDFHVRRHIVLARKLFEAEGTGVHFHISLMGGNIVTAEVTNVR + VDAGADFAAVRMLALFGAVVPH* +>P1; 556 + 795 1 + + + SSTQVRRASAQTSSLQLESIVAVVNVEVFLAAKHSRFYIAVLFAQFGPLLDARLDRGCGK + GAGRRDQWRGGGVDLANGR* +>P1; 796 + 987 1 + + + FGYADHAFHLRSTSRHSDNVKFDSAGRRRCCCFHLVFSLGSDEEGLLARLLVEVTTIRVV + LRG* +>P1; 2 + 163 2 + + NSVWAWCEVPRDYCAAAAGAGGAEVVNGPRDPLDEDVDDEEEVDSALLVAGSD* +>P1; 176 + 391 2 + + PLRSGGGGVEAPETPSGWPARFAAATVANAVEGFSILWMIFTCAVILSLRVNSLKQKGQG + YTFTFRLWEVT* +>P1; 476 + 628 2 + + SLTEPSASPSPTLLLRFSLVLTEGVPNPALRFGVLPLRPAAFNLNPSLLL* +>P1; 629 + 958 2 + + MSRYSWLLNTAGFTSPFCLPSLGRFWTRGLTVAVEKEPAGETNGVEAALTLPMGVSLGML + TMLFTCAPPAAIPIMLSLIPLAAAAAAVSTWCFLWAAMRKACWRACSLR* +>P1; 3 + 293 3 + + IRFGLGVRCPEITAPQLLVLAVRRSSTDPGIRWTRTSTTRRRWIAHCWWLAATDLSSDHS + DPAAEASRLPKLPVAGLLDSLPRLWPTPSRDFRSCG* +>P1; 411 + 521 3 + + CACRRGSRLCSGTYARPLWCSSPSLSPPPRPRQRCC* +>P1; 1020 + 37 1 - + EFGKYNPLTDNSSPTQDHTDGSHLNEQARQQAFLIAAQRKHQVETAAAAAASGIKLNIIG + MAAGGAQVKSMVSIPKLTPIGKVNAASTPLVSPAGSFSTATVKPRVQKRPKLGKQNGDVK + PAVFSSQEYLDIYNSNDGFKLKAAGLSGSTPNLSAGLGTPSVKTKLNLSSNVGEGEAEGS + VRDYCTKEGEHTYRCKVCSRVYTHISNFCRHYVTSHKRNVKVYPCPFCFKEFTRKDNMTA + HVKIIHKIENPSTALATVAAANLAGQPLGVSGASTPPPPDLSGQNSNQSLPATSNALSTS + SSSSTSSSSGSLGPLTTSAPPAPAAAAQ* +>P1; 373 + -1 2 - + AKCESVPLSLLLQRVYAQGQYDGARENHPQDRKSLDGVGHSRGSESSRPATGSFGSLDAS + AAGSEWSELKSVAASHQQCAIHLLLVVDVLVQRIPGSVDDLRTASTSSCGAVISGHLTPS + PNRI* +>P1; 517 + 407 2 - + QQRWRGRGGGLSEGLLHQRGRAYVPLQSLLPRLHAH* +>P1; 649 + 518 2 - + QPGIPRHLQQQRWIQVEGCWSERKHAEPECWIRNSLCQNQAES* +>P1; 853 + 650 2 - + HYRNGGWWSAGEKHGQHTQTNAHWQGQRRLHAIGLACRLLFHSHGQAARPEAAQTQTER + RCKTGCV* +>P1; 958 + 854 2 - + SPQRAGAPTSLPHRCPEKTPGGNSSSGGGQRNQT* +>P1; 179 + 78 3 - + VVRTQISRCQPPAMRYPPPPRRRRPRPADPWVR* +>P1; 479 + 363 3 - + GTTAPKRASIRTAAKSAPASTRTLVTSAVTMLPPISEM* +>P1; 791 + 666 3 - + RPLARSTPPPRHWSRLPAPFPQPRSSRASRSGPNWANRTAM* +>P1; 1022 + 819 3 - + SNSASTTRSPTTAHPRRTTRMVVTSTSRRANKPSSSLPRENTRWKQQQRRRPAESNLTLS + EWRLVERR* +End of file +.end lit + +.LEFT MARGIN1 +@41. TX 5 @ Calculate and write codon table to disk +.LEFT MARGIN2 +.para +This routine calculates codon usage tables +for sections of the sequence +and stores the resulting tables on disk. +The sections to translate can be +defined from the keyboard or by supplying the name of the appropriate +EMBL +library feature table. +.para +If required users can add to an existing codon table stored as a disk file. +Choose between storing observed counts or having them normalised so +that the totals for each amino acid sum to 100. Select between defining +segments at the keyboard or using an EMBL feature table. Define +segments. Signal completion with a zero start. Supply a file name. For +each segment the program will display the counts, at the end it will +display the accumulated totals. +.lit + + Typical dialogue follows. +? Menu or option number=41 + Calculate and write codon table to disk +? (y/n) (y) Start with empty table +? (y/n) (y) Show observed counts +? (y/n) (y) Define segments using keyboard +? Count from (0-1023) (0) =1 +? Count to (1-1023) (1023) =111 +? (y/n) (y) + strand + + =========================================== + F TTT 0. S TCT 0. Y TAT 0. C TGT 0. + F TTC 1. S TCC 1. Y TAC 0. C TGC 3. + L TTA 1. S TCA 0. * TAA 0. * TGA 1. + L TTG 2. S TCG 0. * TAG 0. W TGG 2. + =========================================== + L CTT 0. P CCT 0. H CAT 0. R CGT 2. + L CTC 0. P CCC 0. H CAC 0. R CGC 2. + L CTA 0. P CCA 0. Q CAA 1. R CGA 1. + L CTG 1. P CCG 0. Q CAG 2. R CGG 2. + =========================================== + I ATT 0. T ACT 0. N AAT 0. S AGT 0. + I ATC 0. T ACC 1. N AAC 0. S AGC 1. + I ATA 0. T ACA 0. K AAA 0. R AGA 1. + M ATG 0. T ACG 0. K AAG 0. R AGG 0. + =========================================== + V GTT 0. A GCT 1. D GAT 0. G GGT 3. + V GTC 0. A GCC 1. D GAC 0. G GGC 1. + V GTA 0. A GCA 0. E GAA 1. G GGA 4. + V GTG 1. A GCG 0. E GAG 0. G GGG 0. + =========================================== +? Count from (0-1023) (0) = + + Codon totals over all genes + =========================================== + F TTT 0. S TCT 0. Y TAT 0. C TGT 0. + F TTC 1. S TCC 1. Y TAC 0. C TGC 3. + L TTA 1. S TCA 0. * TAA 0. * TGA 1. + L TTG 2. S TCG 0. * TAG 0. W TGG 2. + =========================================== + L CTT 0. P CCT 0. H CAT 0. R CGT 2. + L CTC 0. P CCC 0. H CAC 0. R CGC 2. + L CTA 0. P CCA 0. Q CAA 1. R CGA 1. + L CTG 1. P CCG 0. Q CAG 2. R CGG 2. + =========================================== + I ATT 0. T ACT 0. N AAT 0. S AGT 0. + I ATC 0. T ACC 1. N AAC 0. S AGC 1. + I ATA 0. T ACA 0. K AAA 0. R AGA 1. + M ATG 0. T ACG 0. K AAG 0. R AGG 0. + =========================================== + V GTT 0. A GCT 1. D GAT 0. G GGT 3. + V GTC 0. A GCC 1. D GAC 0. G GGC 1. + V GTA 0. A GCA 0. E GAA 1. G GGA 4. + V GTG 1. A GCG 0. E GAG 0. G GGG 0. + =========================================== +? (y/n) (y) Save table in a file n +.end lit + +.left margin1 +@42. TX 6 @ Codon usage method +.LEFT MARGIN2 +.para +Used to find protein coding regions. For each window length of the +sequence the routine measures the closeness to an expected codon usage. +Results are plotted for each of the three reading frames. Stop and start +codons are also marked on the plots. Has the highest resolution of all +such methods, but makes the strongest assumption, i.e. that the codon +usage is known. The latest version is described in Methods in Enzymology +183, 193-211. +.para +Choose whether to use an internal standard (i.e. part of the current +sequence known to code for a protein). If so define its end points, and +those of any others. Otherwise supply the name of a disk file containing a +table of codon usage. Tables are listed. Choose between using the +observed counts, or two types of normalisation: normalised to give an +average amino acid composition; normalised to no amino acid bias. The +first normalisation is clearly often sensible, but the second removes +valuable information and is only made availabe for special +circumstances. The final table will be displayed, followed by the +expected scores for window lengths 21, 31 and 41 codons. The scores for +each of the three reading frames are shown (they are logarithmic values) +to help users choose a window length for the analysis. Define a window +length and plot interval. Plotting will start. +.para +The method was first described in +Staden and McLachlan Nucl. Acid Res. 10 141-156 (1982) and the +following is a summary of the initial ideas. +The method makes the following main assumptions: the codon +preferences +of all the +genes in the sequence we are examining are similar to those of the +standard; +the sequence is coding +throughout its whole length in only one reading frame; in the coding +frame +the frequency of codon abc has a definite value Fabc +.LEFT MARGIN2 +If we select a sequence a1b1c1a2b2c2a3b3c3,...,anbncnan+1bn+1cn+1 +then the +probability of selecting it in each of the three frames is: +.left margin15 +frame 1: p1=Fa1b1c1.Fa2b2c2....Fanbncn +.left margin15 +frame 2: p2=Fb1c1a2.Fb2c2a3...Fbncnan+1 +.left margin15 +frame 3: p3=Fc1a2b2.Fc2a3b3...Fcnan+1bn+1 +.LEFT MARGIN2 +The probability that selection of a particular sequence was "caused" by it +being a coding sequence is: +.LEFT MARGIN2 +P1=p1/(p1+p2+p3), P2=p2/(p1+p2+p3), P3=p3/(p1+p2+p3). +.LEFT MARGIN2 +The program calculates these values for the given window length but +plots +Log(P/(1-P)) for each of the three frames. At each point along the +sequence +that the program has a +point to plot it finds which of the three values is highest and places a +single point at the 50% level for the corresponding frame. These single +points will join to form a solid line if one frame is consistently the +highest scoring. In addition stop codons are shown as short vertical lines +that bisect the 50% +level of probability. When looking for coding regions +the user should look for solid horizontal lines at the +50% level that are not interrupted by these short vertical lines. +.para +Changes. + Two normalisations are offered: 1) to remove all amino acid +compositional components from the tables, hence leaving only the codon +preference components. In general this is not recommended as the amino +acid +component alone is often sufficient to choose correctly between frames, +but +may be useful in special circumstances. 2) to change the amino acid +composition components to give an average amino acid composition +rather the +the one contained in the standard (this leaves the codon preference +components unchanged). In general this should be useful as the average +amino acid composition is likely to be closer to the composition of the +genes being hunted, than is that of the standard table of codon +preferences. +The average composition +is that recently published by Argos, not the Dayhoff one that we have +used +before. +.para +Typical dialogue follows. +.lit + +? Menu or option number=42 +Staden and McLachlan codon usage method +Codon tables for standards may be read from disk +or calculated from parts of the current sequence +? (y/n) (y) Define internal standard +Define standard +? start (0-1023) (0) =1 +? end (2-1023) (1023) =1000 + =========================================== + F TTT 13. S TCT 1. Y TAT 1. C TGT 3. + F TTC 4. S TCC 10. Y TAC 1. C TGC 7. + L TTA 1. S TCA 0. * TAA 1. * TGA 4. + L TTG 4. S TCG 1. * TAG 3. W TGG 5. + =========================================== + L CTT 9. P CCT 1. H CAT 3. R CGT 14. + L CTC 7. P CCC 0. H CAC 7. R CGC 14. + L CTA 0. P CCA 0. Q CAA 4. R CGA 9. + L CTG 12. P CCG 1. Q CAG 9. R CGG 8. + =========================================== + I ATT 7. T ACT 4. N AAT 4. S AGT 1. + I ATC 4. T ACC 5. N AAC 3. S AGC 7. + I ATA 1. T ACA 1. K AAA 3. R AGA 2. + M ATG 2. T ACG 1. K AAG 2. R AGG 2. + =========================================== + V GTT 11. A GCT 13. D GAT 6. G GGT 9. + V GTC 5. A GCC 10. D GAC 9. G GGC 11. + V GTA 6. A GCA 5. E GAA 6. G GGA 12. + V GTG 8. A GCG 5. E GAG 3. G GGG 8. + =========================================== +Define standard +? start (0-1023) (0) = +Total codons in standard= 333. +X 1 Use observed frequencies + 2 Normalize to average amino acid composition + 3 Normalize to no amino acid bias +? 0,1,2,3 =2 + =========================================== + F TTT 19. S TCT 2. Y TAT 10. C TGT 3. + F TTC 6. S TCC 22. Y TAC 10. C TGC 8. + L TTA 2. S TCA 0. * TAA 0. * TGA 0. + L TTG 7. S TCG 2. * TAG 0. W TGG 8. + =========================================== + L CTT 16. P CCT 16. H CAT 4. R CGT 10. + L CTC 12. P CCC 0. H CAC 10. R CGC 10. + L CTA 0. P CCA 0. Q CAA 8. R CGA 7. + L CTG 21. P CCG 16. Q CAG 18. R CGG 6. + =========================================== + I ATT 19. T ACT 13. N AAT 16. S AGT 2. + I ATC 11. T ACC 17. N AAC 12. S AGC 15. + I ATA 3. T ACA 3. K AAA 22. R AGA 1. + M ATG 15. T ACG 3. K AAG 15. R AGG 1. + =========================================== + V GTT 15. A GCT 21. D GAT 14. G GGT 10. + V GTC 7. A GCC 16. D GAC 20. G GGC 13. + V GTA 8. A GCA 8. E GAA 26. G GGA 14. + V GTG 11. A GCG 8. E GAG 13. G GGG 9. + =========================================== +Span length 21 expected mean values: 4.8 -5.7 -4.8 +Span length 31 expected mean values: 7.1 -8.4 -7.2 +Span length 41 expected mean values: 9.5 -11.1 -9.5 +? odd span length (11-101) (25) =41 +? plot interval (1-11) (5) = + + Missing graphics display here + +.end lit + +.left margin1 +@43. TX 6 @ Positional base preference method. +.LEFT MARGIN2 +.para +Used to find protein coding regions. For each window length of the +sequence the routine measures the closeness to an expected pattern of +base frequencies . Results are plotted for each of the three reading +frames. Stop and start codons are also marked on the plots. The method +is particularly useful for showing which reading frame is the most likely +to be coding. The latest version is described in a forthcoming issue of +Methods in Enzymology, but the original ideas were given in +Staden, R. Nucl. Acid Res. 12 551-567 (1984). +.para +If dialogue is requested the following inputs are needed, otherwise the +standard analysis is performed. Choose between a "global" standard, or a +selected one. If the global standard is selected the +expected scores are displayed and the user asked to define a span length +and a plot interval. Then users choose between plotting relative or +absolute scores, and can reset the scaling values employed for plotting. +If the global standard is not selected users must define a region of the +sequence to use as a standard, or they can read in a codon table from which +the +program will calculate one. Then they can either, use the values +observed in this standard, or they can combine its values for the third +positions in codons, with those from the global standard. Next they can +give different weightings to each of the three positions in codons. +.para +In its original form the method + took advantage of the +uneven +use of amino acids by proteins and the structure of the genetic code table +and assumed that there is a typical ("global") +amino acid composition +and no codon preference. The typical amino acid composition is the +average +composition found by Argos (see below). + This composition and no codon preference +determines the frequency of each of the four bases in each of the three +codon positions. This 3x4 frequency table shows unequal use of the bases +and in particular a marked use of G in position 1 and of A in position 2 +(at the expence of G). The routine slides a window along the sequence and +calculates a score for each of the three reading +frames at each window position. It assumes the sequence is coding +throughout its whole length and calcualtes the probability that it is +coding in each of the three frames. +When tested against all the E. coli sequences in the EMBL sequence +library +it correctly identified the coding frame for 91% of window positions. +(The E. coli +sequences were chosen only for technical reasons: I have no reason to +think +the method would work less well on other organisms with roughly even +base composition.) +The routine can plot either absolute or relative values: ie absolute values +are the values found by summing the scores for each frame (say p1, p2 +and +p3), and the relative values are then p1/(p1+p2+p3), p2/(p1+p2+p3) and +p3/(p1+p2+p3). +.para +At each point along the sequence +that the program has a +point to plot it finds which of the three values is highest and places a +single point at the 50% level for the corresponding frame. These single +points will join to form a solid line if one frame is consistently the +highest scoring. In addition stop codons are shown as short vertical lines +that bisect the 50% +level of probability. When looking for coding regions +the user should look for solid horizontal lines at the +50% level that are not interrupted by these short vertical lines. + +The absolute mean +values expected on the complement of +the coding strand (and in the same frame) +are 5% lower than those on the coding strand but the relative values +are the same on both strands. Although the +relative values give smoother plots and tend to emphasize the coding +frame +they therefore, cannot be used to decide which strand is coding. The +absolute values plot should be used for this purpose but bearing in mind +the fact the the differences between strands are quite small. +.para +The method has been improved in two overall ways: first it now allows +users to +define their own typical amino acid composition by selecting a standard +sequence from within the sequence they are analysing or from a codon table; +secondly it allows the inclusion of third position preferences. +Again these third position preferences are defined by the use of an +internal standard sequence. Not only can users define their own standards +but they can also give weights to each of the three positions in codons. +This allows different emphasis to be used for each of the three positions. +As an example of its use, by giving, in turn, weights of 1.0, 0.0, 0.0, and +0.0, 1.0, 0.0, and finally 0.0, 0.0, 1.0, you could see the separate +contribution made by each of the three positions. It is also possible to +use the third position preferences with the values for the first two +positions taken from the "global" amino acid composition. + In all cases users may choose to plot +absolute or relative values. The expected scores are displayed before +each +analysis and scales are drawn on the plots. +At present this method does not give probabilities of coding; it has only +been tested for its ability to choose the correct reading frame (see +above). It could be used to give probabilities of coding if was applied to +all known coding and non-coding sequences in the way that the uneven +positional base frequencies method was. It is designed to be used in +conjunction with this method. Note that the average amino composition +used +to derive the base frequencies was changed on 17-11-1988, to be + the new average given by McCaldon and Argos in Proteins 4 99-122 +(1988). +A further change is to allow users to select their own scales for +producing the plots. It can be helpful if they want to emphasise or +diminish +certain features. +.para +Typical dialogue follows. +.lit +? Menu or option number=D43 +Positional base preferences method to find protein genes +Select standard source +X 1 Use global standard + 2 Use internal standard + 3 Use codon usage table +? Selection (1-3) (1) =2 +Define region for standard +? start (0-8134) (0) =3171 +? end (3172-8134) (8134) =4700 +Select normalisation +X 1 Use observed frequencies + 2 Combine with global standard +? Selection (1-2) (1) =1 + T C A G Range + 1 0.125 0.249 0.230 0.397 0.272 + 2 0.298 0.245 0.292 0.165 0.132 + 3 0.288 0.313 0.169 0.230 0.144 +? (y/n) (y) Use 1.0 for positional weights +Give weights between 0.0 and 1.0 +to each of the 3 codon positions +? Position 1 (0.00-1.00) (1.00) = +? Position 2 (0.00-1.00) (1.00) = +? Position 3 (0.00-1.00) (1.00) = +Expected scores per codon in each frame + 0.136 0.122 0.123 +? odd span length (31-101) (67) = +? plot interval (1-11) (5) = +? (y/n) (y) Plot relative scores +Scaling values: + Minimum maximum range + 0.3121 0.3656 0.0382 +? (y/n) (y) Leave scaling values unchanged + + Graphics not shown + +? Menu or option number=D43 +Positional base preferences method to find protein genes +Select standard source +X 1 Use global standard + 2 Use internal standard + 3 Use codon usage table +? Selection (1-3) (1) =3 +? File name of standard=atpase.cods + =========================================== + F TTT 21. S TCT 33. Y TAT 15. C TGT 5. + F TTC 55. S TCC 40. Y TAC 40. C TGC 4. + L TTA 8. S TCA 7. * TAA 8. * TGA 0. + L TTG 19. S TCG 12. * TAG 1. W TGG 17. + =========================================== + L CTT 22. P CCT 17. H CAT 6. R CGT 73. + L CTC 21. P CCC 4. H CAC 30. R CGC 23. + L CTA 1. P CCA 10. Q CAA 19. R CGA 5. + L CTG 168. P CCG 48. Q CAG 80. R CGG 3. + =========================================== + I ATT 47. T ACT 14. N AAT 17. S AGT 8. + I ATC 98. T ACC 54. N AAC 52. S AGC 26. + I ATA 6. T ACA 7. K AAA 85. R AGA 0. + M ATG 75. T ACG 13. K AAG 28. R AGG 0. + =========================================== + V GTT 67. A GCT 56. D GAT 41. G GGT 90. + V GTC 29. A GCC 53. D GAC 66. G GGC 66. + V GTA 49. A GCA 59. E GAA 101. G GGA 5. + V GTG 57. A GCG 64. E GAG 41. G GGG 8. + =========================================== +Select normalisation +X 1 Use observed frequencies + 2 Combine with global standard +? Selection (1-2) (1) =2 + T C A G Range + 1 0.177 0.211 0.277 0.336 0.159 + 2 0.271 0.238 0.310 0.182 0.128 + 3 0.242 0.301 0.168 0.289 0.132 +? (y/n) (y) Use 1.0 for positional weights +Expected scores per codon in each frame + 0.785 0.736 0.736 +? odd span length (31-101) (67) = +? plot interval (1-11) (5) = +? (y/n) (y) Plot relative scores +Scaling values: + Minimum maximum range + 0.3219 0.3519 0.0214 +? (y/n) (y) Leave scaling values unchanged + + Graphics not shown +.end lit +.left margIN1 +@44. TX 6 @ Uneven positional base frequencies. +.LEFT MARGIN2 +.para +Used to find regions of a sequence that might be coding for a protein. The +method looks for sections of the sequence in which the frequency at +which each of the four bases occupies the three positions in codons is +nonrandom. The level of nonrandomness is plotted on a scale that shows +the probability that the sequence is coding. At each position along a +sequence the calculation gives the same value for all six possible reading +frames, so only one value is plotted. +.para +Define the window length and plot interval. +.para +The results are plotted in a box divided by a horizontal line marked "76%". +76% of coding regions achieve values above this line and 76% of +noncoding regions achieve scores below the line. +.para +This method, first described in Staden R. Nucl. Acid Res. 12 551-567 +1984, +looks for uneven positional +usage of bases in codons. +It looks through the sequence in one fixed +phase and counts the number of times each base apears in each of the +three +codon positions: for each window position it counts A1,A2,A3 and +C1,C2,C3 +and G1,G2,G3 and T1,T2,T3 and calculates AMEAN=(A1+A2+A3)/3, and +similarly +CMEAN, GMEAN +and TMEAN; it then calculates +ADIF=abs(A1-AMEAN)+abs(A2-AMEAN)+abs(A3-AMEAN) and similarly +CDIF, GDIF and +TDIF to measure the differences between an even base usage for all +positions in the codons and the observed usage. The routine then +calculates +the sum ADIF+CDIF+GDIF+TDIF and plots this value on the following scale: +the base level is such that no known window in a coding region has a +lower +value, whereas 14% of windows in noncoding sequences score below it. +The +top of the scale is not achieved by any known noncoding +region, but is reached by 16% of known coding regions. +The bar drawn across the +plot corresponds to a level that is exceeded by 76% of windows in known +coding regions +but is reached by only 24% of windows in known noncoding regions. ie +76% of +coding windows score above and 76% of noncoding windows score below. +This is similar to Ficketts method but without +the probabilities and weightings from the Los Alamos sequence library: it +is therefore unbiased but may well give very similar results. +.left margin1 +@45. TX 6 @ Codon improbability on base composition +.LEFT MARGIN2 +.para +Used to find regions of a sequence that might code for a protein. +.para +If dialogue is requested define a window length and plot interval. +.para + The idea of the method is, that of all sequence features +that we know, it is only +coding regions that will give rise to codon biases well above those +expected +from the base composition. +If a region of sequence shows sufficiently strong +codon bias then we conclude that it is coding for a protein. + Using the multinomial distribution we +have derived a function to measure the improbability of observing a +set of codons from a sequence of the given composition. Using the +Poisson +distribution we have worked out the distribution +of the improbability. The program plots the observed improbability minus +the expected improbability (the mean as calculated from the Poisson +distribution). The plots are presented against a scale of units of standard +deviation as measured from the Poisson distribution. As with the other +Staden and McLachlan method the program puts an extra point at a fixed +level for the highest of the three probabilities; for this function this +point is placed at six standard deviations above the mean expected level. +The top of each plot corresponds to 12 standard deviations above the +expected level and the bottom corresponds to the expected value. +.para +Analysis of the application +of the method to the EMBL sequence library indicates that the method +does +work for most sequences and that the levels of improbability roughly +correlate with levels of expression. +Coding regions will show high peaks in all three frames making +interpretation more difficult than for some of the other methods. +.left margin1 +@46. TX 6 @ Codon improbability on amino acid composition +.LEFT MARGIN2 +.para +Used to finds regions of a sequence that might code for a protein. +.para +If dialogue is requested define a window length and a plot interval. +.para +The idea of the method is, that of all sequence features +that we know, it is only +coding regions that will give rise to codon biases such that, for each +amino acid, some codons are used far more frequently than others. The +method is independent of what the bias actually is, requiring only that it +is present. +If a region of sequence shows sufficiently strong +codon bias then we conclude that it is coding for a protein. + Using the multinomial distribution we +have derived a function to measure the improbability of observing a +set of codons from a sequence of the given composition. Using the +Poisson +distribution we have worked out the distribution +of the improbability. The program plots the observed improbability minus +the expected improbability (the mean as calculated from the Poisson +distribution). The plots are presented against a scale of units of standard +deviation as measured from the Poisson distribution. As with the other +Staden and McLachlan method the program puts an extra point at a fixed +level for the highest of the three probabilities; for this function this +point is placed at six standard deviations above the mean expected level. +The top of each plot corresponds to 12 standard deviations above the +expected level and the bottom corresponds to the expected value. +.left margin1 +@47. TX 6 @ Shepherd RNY preference method +.LEFT MARGIN2 +.para +Used to find regions of a sequence that might code for a protein. Based on +the method of Shepherd +(PNAS 78 1596-1600, 1981). +.para +If dialogue is requested define a window length and plot interval. +.para +Shepherd has found that +many genes have a preference for the use of codons of the form RNY +where +R=purine, Y=pyrimidine and N=any base. He has attributed this to being +due +to remants of a primitive genetic code. The calculation is similar to that +for the Staden and McLachlan method, the p1's being simply the number of +RNY codons found in frame 1 etc and the P's being p/(p1+p2+p3). +.left margIN1 +@48. TX 6 @ Ficketts method +.LEFT MARGIN2 +.para +Used to find regions of a sequence that might code for a protein. Based on +the method of Fickett +(Nucl. Acid Res.10 +1982), but plots values for fixed window lengths rather than over the +whole of open reading frames. +.para +If dialogue is requested define a window length and plot interval. The +results are plotted in a box divided into three horizontal strips. +.para +Sections of the sequence with values plotted in the top strip of the box +are adjudged to be coding, those in the middle strip "no decision", and +those in the bottom "not coding". +.para +The program performs the following calculations: let A1 = the number of +occurences of base A in position 1 of codons, A2 for position 2 etc. +Similarly for bases C,G and T. For each window position calculate +Apos=max(A1,A2,A3)/min(A1,A2,A3)+1. Similarly for C,G and T to give 4 +positional values. Also count the base composition for the window to +give +Acomp, Ccomp etc. Fickett tested each of these 8 parameters singly as +to +their ability to distinguish coding from noncoding regions and arived at +probabilities of coding for the range of values each can take = Pcod. He +also measured their relative abilities and given weightings to each of +the 8 parameters = Pw. To calculate the "TESTCODE" for a window we +first lookup the Pcod for each of the calculated compositional and +positional values and then calculate TESTCODE=sum(Pcod*Pw). TESTCODE +is +plotted relative to three levels of decision: the top division="coding", +the middle="no opinion" and the bottom division="non coding". +.left margin1 +@49. TX 6 @ tRNA gene search. +.LEFT MARGIN2 +.para +Used to find segments of a sequence that might code for tRNAs. Looks for +potential cloverleaf forming structures and then for the presence of +expected conserved bases. Presents results graphically or draws out the +cloverleafs. +.para +If dialogue is requested a large number of parameters need to be given +values, including some loop lengths, scores for each of the four stems, +and scores for the conserved bases. +.para +The program was first described in +Staden Nucl. Acid Res 817-825 (1980). + The tRNA's that have + been sequenced so far have two characteristics that can be used +to + locate their genes within long DNA sequences. Firstly they have a + common secondary structure - the cloverleaf - and secondly, + particular bases almost always appear at certain positions in +the + cloverleaf. The cloverleaf is composed of four base-paired +stems + and four loops. Three of the stems are of fixed length but the + fourth, the dhu stem which usually has four base pairs, +sometimes + has only three. All of the loops can vary in size. The following + relationships between the stems in the cloverleaf are assumed in +the + program: (a) there are no bases between one end of the +aminoacyl + stem and the adjoining tuc stem; (b) there are two bases +between + the aminoacyl stem and the dhu stem; (c) there is one base +between + the dhu stem and the anticodon stem; (d) there are at least three + bases between the anticodon stem and the tuc stem. + The program looks first for cloverleaf structure and then, if + required, for conserved bases. The sizes of the loops, the number + of basepairs in the stems and the required conserved bases may +all + be specified by the user. The process of looking for the presence + of conserved bases can reduce the number of potential +structures + found considerably. + The + user may also specify that an intron may be present in the +anticodon + loop. +.para +The user may define a minimum number of +base pairs for each stem using the scoring system G-C, A-T=2 and G-T=1 +and +scores for each of the conserved bases. Recommended values for the stem +scores are given by the prompts and the percentage conservation of the +conserved bases as found in the Nucl. Acid Res 1979 paper Gauss, Gruter + and Sprinzl are also given, +but the user must decide which bases are most +likely to be conserved for the sequence being examined. +The output shows the position of the possible gene in the sequence by a +vertical line the height of which shows the number of basepairs made in +the +stems. The cloverleaf structure is also drawn but will scroll up off the +screen. Output of the cloverleafs will look like: +.lit + + 6942 + A + A-U + A-U + G-C + A-U + U-A + A-U + U-A AAU + U UAUCU + AA A !!!!! + AAUG AUAGA A + U !!!! U UCA + C UUAC U + AA A + U-AA A + A-U + A-U + C-G + U-A + U A + U A + GUC + + Typical dialogue follows. + +? Menu or option number=D49 + tRNA search +? Maximum trna length (70-130) (92) = +? Aminoacyl stem score (0-14) (11) = +? Tu stem score (0-10) (8) = +? Anticodon stem score (0-10) (8) = +? D stem score (0-8) (3) = +? Minimum base pairing total (30-32) (32) = +? Minimum intron length (0-30) (0) = +? Minimum length for TU loop (4-12) (6) = +? Maximum length for TU loop (6-12) (9) = +? (y/n) (y) Skip search for conserved bases n +Give a score for each base, then a minimum total at the end +? Base 8, T is 100% conserved. Score (0-100) (0) = +? Base 10, G is 95% conserved. Score (0-100) (0) = +? Base 11, Y is 96% conserved. Score (0-100) (0) = +? Base 14, A is 100% conserved. Score (0-100) (0) = +? Base 15, R is 100% conserved. Score (0-100) (0) = +? Base 21, A is 97% conserved. Score (0-100) (0) = +? Base 32, Y is 100% conserved. Score (0-100) (0) = +? Base 33, T is 98% conserved. Score (0-100) (0) = +? Base 37, A is 91% conserved. Score (0-100) (0) = +? Base 48, Y is 100% conserved. Score (0-100) (0) = +? Base 53, G is 100% conserved. Score (0-100) (0) = +? Base 54, T is 95% conserved. Score (0-100) (0) = +? Base 55, T is 97% conserved. Score (0-100) (0) = +? Base 56, C is 100% conserved. Score (0-100) (0) = +? Base 57, R is 100% conserved. Score (0-100) (0) = +? Base 58, A is 100% conserved. Score (0-100) (0) = +? Base 60, Y is 92% conserved. Score (0-100) (0) = +? Base 61, C is 100% conserved. Score (0-100) (0) = +? Minimum total conserved base score (0-0) (0) = +? (y/n) (y) Plot results n + + Searching + + 306 + C + C-G + C-G + G-C + T-A + C-G + A-T + T+G AT + A ATACA + TTC T !!!! G + CTGT TATGG G + G ! ! T GA + C TAAA C + GCG C G + T+GA C + C-G C T + T+G A T + T-A G T + T-A G A + G G G C + A A G A + AGC T C + A T + C T + A + C T + + +.end lit +.left margIN1 +.left margIN1 +@50. TX 7 @ Plot start codons +.left margin2 +.para +This function plots the positions of all start codons for each of the three +reading frames. +.left margin1 +@51. TX 7 @ Plot stop codons +.left margin2 +.para +This function plots the positions of all stop codons for each of the three +reading frames. +.left margIN1 +@52. TX 7 @ Plot stop codons on the complementary strand +.left margin2 +.para +This function plots the positions of all stop codons for each of the three +reading frames on the complementary strand. +.left margin1 +@53. TX 7 @ Plot stop codons on both strands +.left margin2 +.para +This function plots the positions of all stop codons for each of the three +reading frames on both strands. +.left margin1 +@54. TX 5 @ Search for longest open reading frames +.left margin2 +.para +This function will report the positons of the ends of +all sections of sequence that contain no stop codons. All six reading +frames are examined. Results are presented in the form of an EMBL feature +table. Hence if the results are stored in a file by use of "direct output +to disk", the file + can be used to translate the +open reading frames in a sequence. +Note that in order for the file to be used as a feature table it +must include either EMBL +or GenBank headers, and a suitable "tail". The simplest header is the word +FEATURES starting in column 1 of the first line of the file. The simplest +tail is 2 empty lines at the end of the file. These lines are not included +when nip writes out results in feature table format. +.para +Define the minimum length of open reading frame to report (in amino +acids). +Choose to search either or both strands. The program displays the end +points, the reading frame and strand. +.para +Typical dialogue follows. +.lit + +? Menu or option number=D54 + Find open reading frames +? Minimum open frame in amino acids (5-1000) (30) =100 + +X 1 + strand only + 2 - strand only + 3 Both strands +? 0,1,2,3 =3 + +FT CDS 1 831 1 831 +FT CDS 1540 2853 1 1314 +FT CDS 3130 4242 1 1113 +FT CDS 5761 6114 1 354 +FT CDS 6187 6711 1 525 +FT CDS 1766 2077 2 312 +FT CDS 2078 2446 2 369 +FT CDS 4136 5500 2 1365 +FT CDS 1335 1637 3 303 +FT CDS 2844 3194 3 351 +FT CDS 6819 7238 3 420 +FT CDS 2073 1711 C 1 363 +FT CDS 2469 2149 C 1 321 +FT CDS 6542 6144 C 3 399 + +.end lit +.left margin1 +@55. TX 8 @ Search for E. coli promoter (general) +.LEFT MARGIN2 +.para +Searches for E coli promoter like sequences using a standard weight +matrix. The positions of the matches are plotted. No dialogue is required. +.para +The method was first described in + Staden R. Nucl. Acid Res. 12 505-519 1984. +This search uses a weight matrix taken from the frequency tables +contained +in Hawley, D. K. and McClure, R., nar 11 2237-2255 (1983). + The weight matrix is +divided into 3 sections that are separated by varying sizes of gap: the - +35 +region, the -10 and the +1 region. +The algorithm first looks for a sufficiently good -35 region, then for the +best -10 region within range and then for the best +1 region within range +of the -10; each separate region must score above the lowest known +score +for the corresponding section. The gap penalty is then applied and two +plots +produced: one with gap penalties, one without. + Scaling is such that no +known promoter scores below the bottom level and no known promoter +scores +above the top level when the weight matrix is applied. +.para +Two other functions also look for E. coli promoters: 92 looks for sites on +the complementary strand and 93 looks for individual -35 and -10 +regions +and plots them on a scale such the top is the highest known value +10% +and +the bottom is the lowest known -10% +.LEFT MARGIN1 +.lit +weights for E. coli promoters +-35 region: +P -50-49-48-47-46-45-44-43-42-41-40-39-38-37-36-35-34-33-32-31-30-29-28-27-26 + +107109109110110110110110110111111110111112112112112112112112112112112112112 +T 41 33 32 25 34 22 35 35 42 27 32 42 47 14 92 94 11 19 15 37 46 34 38 48 34 +C 22 27 18 29 20 14 20 12 22 23 16 25 10 43 7 6 11 18 60 8 25 23 23 17 20 +A 28 38 30 37 35 56 42 42 37 42 39 18 25 26 2 6 2 72 26 50 26 34 25 26 31 +G 16 11 29 19 21 18 13 21 9 19 24 26 29 29 11 6 88 3 11 17 15 21 26 21 27 +-10 region: +P -23-22-21-20-19-18-17-16-15-14-13-12-11-10 -9 -8 -7 -6 -5 + 112112112112112112112112112112112112112112112112112112112 +T 35 28 28 27 39 51 34 43 26 31 89 3 49 15 19108 31 29 21 +C 34 21 24 27 12 25 20 25 20 27 10 2 16 14 22 3 13 16 30 +A 20 39 33 33 39 23 29 16 23 19 2106 29 66 57 1 35 23 31 +G 23 24 27 25 22 13 29 28 43 35 11 1 18 17 14 0 33 24 30 ++ region: +P -2 -1 1 2 3 4 5 6 7 8 9 10 + 86 88 85 88 88 88 88 88 88 88 88 88 +T 16 22 2 42 27 23 20 25 27 15 16 29 +C 29 49 4 25 25 13 18 22 17 17 16 17 +A 20 9 45 16 24 25 28 24 24 32 35 26 +G 21 8 37 5 12 27 22 17 20 24 21 16 +.end lit +Notes: +E. coli promoters have been shown to contain 2 regions of conserved +sequence +located about 10 and 35 bases upstream of the transcription startsite. +These +are TATAAT and TTGACA with an allowed spacing of 15 to 21 bases +between. The +spacing with maximum efficiency was 17 bases and all but 12 of the 112 +sequences could be aligned with a separation of 17 +or-1 bases. The +standard +promoter has spacing 7 and 17 bases between the startsite and the -10 +region, +and the -10 and -35 regions, respectively. The spacing between the -10 +region +and the startsite is usually 6 or 7 bases but varies between 4 and 8 +bases. +There is an AT rich region of 8 to 10 bases upstream of the -35 region. +Iniation with a purine is highly prefered with G being used if A is not +present. +.lit +Gap penalties: + 15 0.02 (only exists as mutant) + 16 0.2 + 17 1.0 + 18 0.2 + 19 0.05 (guess) + 20 0.02 (guess) + 21 0.01 (guess) +.end lit +.left margin1 +@56. TX 8 @ Search for E. coli promoter (general) +strand +.LEFT MARGIN2 +.para +This function searches for E. Coli promoters on the complementary strand +of +the sequence. See the notes on option 55. +.left margin1 +@57. TX 8 @ Search for E. coli promoter sequences. (-35 and -10) +.LEFT MARGIN +.para +This function searches separately for the -35 and -10 sequences of an E. +coli promoter. See the notes on option 55. +.left margIN1 +@58. TX 8 @ Search for procaryotic ribosome binding sites +.LEFT MARGIN2 +.para +This function searches for the 5' ends of prokaryotic genes using an +unusual weight matrix. The search is relatively slow because the matrix +is 101 bases in length. No dialogue is required. +.para +The method was first described in + Staden Nucl. Acid Res. 12 505-519 1984. This actually looks for more +than +a ribosome binding site as is explained below. This uses their weight +matrix w101 of Stormo and +Schneider (NAR 10 2971-3024, 1982) +which with a value of 2 finds all gene starts in their library. +.LEFT MARGIN1 +.lit + P-60-59-58-57-56-55-54-53-52-51-50-49-48-47-46-45-44-43-42-41-40-39-38-37-36 + T 5 1 -3 9-14 7 15 -5 3-16-17 4 18 5 -3 -1 2 4 5 -5 7 8 -5-15 6 + C-21 -6-11-21 0 8 -7-12 -1 1 0-19 12 -3 -1 10 2 -8 -5-11 8 1 23 6 -5 + A 7 -2 13 -2 -8-13-18 5 0 -5 13 8-15 9 -4 -7 9 0 -8-11-10 -6 -7 -5 -6 + G -6 -9 -7 0 8-16 -4 -2-16 1 -4 8-14 5 11-13-24 3 7 22-11 -9-15 10 -4 + + P-35-34-33-32-31-30-29-28-27-26-25-24-23-22-21-20-19-18-17-16-15-14-13-12-11 + T 3 4 16 -4 7 11 -4 -1 12 8 10 -1 1 8 2-10-16 11 1 -3 16 -3-36 -8-27 + C 2-14 -3 -8-10-21 2 0 -2 -1-11 -3 -1 5-11 -4 7 0-14 6 -8-20 -7-36-44 + A-12 -1-27 -3 -6 0-12 -3 -4 -7 14 -2 -4 -6 0 12 5 -9 0-11-11 10 8 2 8 + G 4 -5 -6 -3 -1 -4 -1 -4-15 0-14 3 10-19 -3-10 -7 -7 7 1 -8 -6 15 21 42 + + P-10 -9 -8 -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 + T-53-27-26-23 2 -7-14-40-28 0-53 75-62-20-40-10-35 -5-12 -1 4 14-23 7 -2 + C-15-50-43-35-38-29-29 1 -9 1-87-55-64-45 11-22-14-20-15-15-10-22 -5 2 6 + A 0 -3 -5 4-20-11 5 6 -2-15 66-69-52 -5 -4 6 8-24 -7-10 -7 13 14 -9-18 + G 35 22 16 -6 -5-15-25-33-28-53-36-50107 -5-37-44-27-15-23-16-29-47-17-29-15 + + P 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 + T-26 1 4 -7 3 -4 0-10 8-18 7-22-21 8 4 -3 -6 7 -8 1 -5-16-16 7 -6 + C 6 -8 19 -7 9 -3 17 -2 3 -9 5 22 22 8 -1 1 18 6 11-10 -8 7 10 0 7 + A 14-12-42 1 -5 -4-32 12-10 20 -6 -1 3 -4 4-10 -1 -2-14 11 14 -3 2-13 5 + G-23 -7 -1 -6-17 -4 0-15-14 -4-17-10 -5-13 -8 10-13-13 9 -4 -3 10 2 4 -8 + + P 40 + T 0 + C 14 + A 5 + G-21 +.END LIT +These come from w101 of Stormo, Schneider, Gold and Ehrenfeucht Nucl. +Acid Res. 10 2997- +3011, 1982. They report that this matrix gives a score of at least 2 for +all +gene starts in their library whereas all other sequences score 1 or less. +.left margin1 +@29. TX 1 @ Reverse and complement the sequence +.LEFT MARGIN2 +.para +Reverses and complements the current active region of the sequence. +.left margin1 +@60. TX 7 @ Search using a dinucleotide weight matrix +.LEFT MARGIN2 +.para +This function performs searches for short sequence +motifs using an appropriate dinucleotide weight matrix. In addition it +can be used to create or modify weight matrices. In order to perform a +search the only input +required is the name of the file containing the weight matrix. +The results can be presented graphically or listed. The graphical +presentation will draw line at the position of any matches found; the +height of the line is proportional to the score. The method is identical to +that using weight matrices derived from nucleotide frequencies, except +that here we use the frequencies of dinucleotides. +.para +For a search, select "use weight matrix", supply the name of the file +containing the weight matrix, and choose between having results plotted +or listed. If dialogue is requested when the function is selected users can +alter the cutoff score employed. +.para +To create a weight matrix several steps are involved. A file containing an +alignment of known motifs is required. (This file must be created before +the current option is selected. The format is a follows: each sequence is +written on a separate line with at least one space at the beginning; each +sequence is terminated by a space character, and can be followed by a +name. The sequences must be aligned.) Supply the name of the file of +aligned sequences. The program reads and displays the sequences. Choose +between "summing logs of weights" or summing weights (i.e. whether to +multiply or add weights). If logs are used all scores will be negative. +Choose if all positions in the set of aligned sequences should be used or +if a mask should be applied. If so selected, define a mask as a string of +symbols, in which symbol - means ignore and any other symbol means +use. E.g. xx-x--abc means use all positions except 3,5 and 6. +.para +The program will calculate weights as the frequencies of the +dinucleotides at each unmasked position in the set of aligned sequences. +These weights are then applied to the set of aligned sequences to give a +range of "observed" scores. The mean and standard deviation of these +scores is displayed. The user is asked to supply several values to be used +when the weight matrix is applied to other sequences: a cutoff score (by +default, the mean minus 3 standard deviations), a top score for scaling +graphical results (by default, the mean plus 3 standard deviations), and a +position to identify (this means that if a particular base within the +motif is used as a "landmark", such as the A of the AG in splice acceptor +sites, then its position will be marked in plots). All these values are +stored along with the weight matrix. Finally supply the name of a file to +contain the weight matrix. +.para +Weight matrices can be "rescaled" using a set of aligned sequences in +much the same ways as a matrix is created. The purpose is to redefine +the cutoff scores, and rescaling does not alter any other values in the +weight matrix file. +.para + The methods have always had to deal with the problem of zeroes in the +matrices. The current versions +employ "Laplaces Law of Succession" in which 1 is +added to each term. + +.lit +Typical dialogue follows. + +? Menu or option number=D60 + + Motif search using dinucleotide weight matrix +X 1 Use weight matrix + 2 Make weight matrix + 3 Rescale weight matrix +? 0,1,2,3 = 2 +? Name of aligned sequences file=[RS.MOTIFS]GCN4.SEQ + + + 1 AGCGTGACTCTTCCCGGAA HIS1 + 2 GAGGTGACTCACTTGGAAG HIS1 + 3 CGGATGACTCTTTTTTTTT HIS3 + 4 ACAGTGACTCACGTTTTTT HIS4 + 5 GTCGTGACTCATATGCTTT ARG3 + 6 TGAATGACTCACTTTTTGG ARG4 + 7 TTCTTGACTCGTCTTTTCT CPA1 + 8 CGAATGACTCTTATTGATG CPA2 + 9 AGAATGACTAATTTTACTA TRP5 + 10 TCGTTGACTCATTCTAATC TRP3 + 11 TTGCTGACTCATTACGATT TRP2 + 12 GAGATGACTCTTTTTCTTT IV1 + 13 GCGATGATTCATTTCTCTG IV2 + 14 TAGATGACTCAGTTTAGTC LEU1 + 15 TAAGTGACTCAGTTCTTTC LEU4 + 16 ATGATGACTCTTAAGCATG ILS1 +Length of motif 18 +? (y/n) (y) Sum logs of weights n +? (y/n) (y) Use all motif positions n +x means use, - means ignore +e.g. xx-x---x-x means use positions 1,2,4,8,10 +? Mask=----XXXXXXXX-------- + Applying weights to input sequences + 1 89.000 AGCGTGACTCTTCCCGGA + 2 91.000 GAGGTGACTCACTTGGAA + 3 93.000 CGGATGACTCTTTTTTTT + 4 90.000 ACAGTGACTCACGTTTTT + 5 94.000 GTCGTGACTCATATGCTT + 6 91.000 TGAATGACTCACTTTTTG + 7 81.000 TTCTTGACTCGTCTTTTC + 8 90.000 CGAATGACTCTTATTGAT + 9 75.000 AGAATGACTAATTTTACT + 10 97.000 TCGTTGACTCATTCTAAT + 11 97.000 TTGCTGACTCATTACGAT + 12 93.000 GAGATGACTCTTTTTCTT + 13 69.000 GCGATGATTCATTTCTCT + 14 90.000 TAGATGACTCAGTTTAGT + 15 90.000 TAAGTGACTCAGTTCTTT + 16 90.000 ATGATGACTCTTAAGCAT +Top score 97.000 Bottom score 69.000 +Mean 88.750 Standard deviation 7.319 +Mean minus 3.sd 66.794 Mean plus 3.sd 110.706 +? Cutoff score (-999.00-9999.00) (66.79) = +? Top score for scaling plots (66.79-999.00) (110.71) = +? Position to identify (0-18) (1) = +? Title=GCN4 DI WTS +? Name for new weight matrix file=3.WTS + +? Menu or option number=D60 + Motif search using dinucleotide weight matrix +X 1 Use weight matrix + 2 Make weight matrix + 3 Rescale weight matrix +? 0,1,2,3 = +? Motif weight matrix file=3.WTS + GCN4 DI WTS +? Cutoff score (-9999.00-9999.00) (66.79) =40 +? (y/n) (y) Plot results n + 15 42.00 CAACCCGCTCACCGACAA + 29 42.00 ACAACAGCTCACCCACGC + 93 46.00 AGCCTTCCTCATCGCTGC + 153 40.00 CAGCGGAATCAAACTTAA + 408 42.00 CGATGGATTCAAGTTGAA + 469 47.00 TTAGGAACTCCCTCTGTC + 493 60.00 AAGCTGAATCTTAGCAGC + 530 43.00 CGGAGGGCTCAGTGAGGG + 542 47.00 TGAGGGACTACTGCACCA + 678 41.00 CTTCTGCTTCAAAGAGTT + 709 47.00 AATATGACGGCGCACGTG + 848 54.00 GTCAGAACTCAAATCAGT + 940 49.00 CCGTTGACGACCTCCGCA + 992 42.00 TGGGCACCTCACACCAAG + + +.end lit +.left margIN1 +@61. TX 8 @ Search for eukaryotic ribosome binding sites +.LEFT MARGIN2 +.para +Searches for eukaryotic ribosome binding sites using weightings derived +from + Sargan,Gregory,Butterworth febs let 147 133-136 1982. No dialogue is +required. First described in Staden Nucl. Acid Res. 12 505-519 1984. + +.LEFT MARGIN1 +.lit +mRNA WTS FOR EUKARYOTES SARGAN,GREGORY,BUTTERWORTH FEBS LET +147 133-136 1982 +P -7 -6 -5 -4 -3 -2 -1 1 2 3 + 102102102102102102102102102102 +T 19 24 31 12 0 18 5 0102 0 +C 20 15 32 65 5 42 52 0 0 0 +A 50 27 27 19 86 36 34102 0 0 +G 6 29 12 6 11 6 11 0 0102 +VIRAL ONLY +P -7 -6 -5 -4 -3 -2 -1 1 2 3 + 41 41 41 41 41 41 41 41 41 41 +T 14 12 16 4 2 13 9 0 41 0 +C 7 3 13 17 7 9 14 0 0 0 +A 15 10 6 10 27 15 9 41 0 0 +G 5 16 6 10 5 4 9 0 0 41 +.END LIT +The Sargan et al paper puts forward the hypothesis that there is an +interaction between +some mRNA leader sequences and a highly conserved structure in the 18S +rRNA +of eukaryotic ribosomes. The attempt to substantiate the hypothesis +includes +a table of base frequencies for sequences immediately 5' to start codons. +They examined 102 sequences and I have used the base frequencies they +found +as a weight matrix for searching for eukaryotic gene starts. I don't yet +know how good this method is. The viral sequences were found to be +slightly +different but the separate table shown here is not used in the program. +.left margin1 +@62. TX 8 @ Search for splice junctions +.LEFT MARGIN2 +.para +Used to search for mRNA splice junctions using a weight matrix. The +default weight matrix is still that derived from the paper of Mount (Nucl. +Acids Res. 10, 459-472). However users may employ their own tables. +By default the positions of possible junctions will +be plotted rather than listed. + The diagram splits the donor plot into 3 horizontal boxes + so that all the +sites marked in any box are from the same reading frame. The acceptor +plot appears above the donor plot and is split in an equivalent way. So +sites marked as donors and acceptors in equivalent boxes are compatible. +i.e. donors from donor box 1 are compatible with acceptors from acceptor box +1, etc. Of course it is the combination of reading frame and splice sites +that really matters, and donors from box 1 can be compatible with acceptors +in box 3 if the reading frame switches. +.para +If dialogue is selected users can employ their own file of weights (see +below for the format), can change the cutoff scores, and can elect to have +the results listed rather than plotted. Listed results show the position +(of the last or first base in the exon), the frame and the matching sequence. +The frequency table shown below is used as a default +weight matrix and AG and GT are obligatory at the appropriate positions. +The plots are scaled so that the top of scale is the highest value achieved +by +a junction sequence in the set used to compile the frequency table, and +the +bottom of the scale is the lowest value achieved by a junction sequence +in +the set used to compile the frequency table. +.para +In the light of current knowledge it would be sensible for users to use +the weight matrix search option (20) +to create matrices that define more specific splice junctions. If so it is +important that the positions "marked" are the last base in the donor exon and +the first base in the acceptor exon. To make a weight matrix suitable for +use with this function follow the instructions for option 20 and create +files for both donor and acceptor sites. Then concatenate the two matrix files +with the donor file first. +Note that any positions in the weight matrix that are +100% conserved will be made obligatory (normally the AG and GT). +.LEFT MARGIN1 +.lit + + Mount donors redone 16-4-91 + 12 3 -16.085 -7.500 + P -2 -1 0 1 2 3 4 5 6 7 8 9 + N 136 136 136 136 136 136 136 136 136 136 136 136 + T 28 8 15 17 0 136 9 16 7 84 30 36 + C 41 60 16 7 0 0 3 13 3 17 28 39 + A 40 56 89 12 0 0 83 91 12 23 53 33 + G 27 12 16 100 136 0 41 16 114 12 25 28 + Mount acceptors redone 16-4-91 + 18 15 -26.142 -14.400 + P -14 -13 -12 -11 -10 -9 -8 -7 -6 -5 -4 -3 -2 -1 0 1 2 3 + N 113 113 113 113 113 113 113 113 113 113 113 113 113 113 113 113 113 113 + T 58 50 57 59 67 56 58 49 47 66 64 31 34 0 0 11 41 31 + C 21 28 34 25 29 33 35 32 42 40 33 25 74 0 0 23 28 41 + A 17 11 11 18 7 17 12 23 15 3 10 29 5 113 0 24 21 21 + G 17 24 11 11 10 7 8 9 9 4 6 28 0 0 113 55 23 20 +.END LIT + +.left margIN1 +@63. TX 7 @ Search using a weight matrix (complementary) +.LEFT MARGIN2 +.para +This function searches the complementary strand of the sequence using +a weight matrix. Many +motifs can bind to either strand of the DNA and this function allows +users to +search the complementary strand without having to change the +orientation of the sequence. See option 20 for more details. +.left margin1 +@64. TX 3 @ Plot observed-expected word frequencies +.LEFT MARGIN2 +.PARA +This option is designed to examine the abundances of short +words in a sequence to see if particular ones are either under or over +represented. It compares the observed and expected frequencies and +plots them along the sequence. There has been some work on the relative +amounts of CG dinucleotides in eukaryotic sequences (eg Bird, Nature +321, +209-213 (1986)) and this new routine can be used to examine such +biases, or +any others that might be interesting. +.para +The user selects a word - say CG -, a window length, and a maximum and +mininum scale for plotting the results. The +program examines each sucessive window length along the sequence, +with each +window overlapping the previous one by windowlength-1. +The program counts the base frequencies in each window, and the number +of +occurrences of the chosen word within the window. Using the base +frequencies it calculates an expected number of occurrences for the +chosen +word (simply by multiplying the relevant frequencies). It plots +observed-expected, and hence will show regions that are rich or depleted +in +the chosen word. The longest allowed word is 9 characters, but the +calculation of the expected frequencies becomes less appropriate as the +word +length increases above 2. +.para +Typical dialogue follows. +.lit + +? Menu or option number=D64 +Plot composition differences (obs-exp)) +Default String=CG +? String= +? odd span length (3-401) (101) = +? plot interval (1-20) (5) = +? Maximum plot value (-6.31-25.25) (6.31) = +? Minimum plot value (-25.25-6.31) (-6.31) = + + Missing graphics display here + +.end lit +.left margIN1 +@65. TX 9 @ Search for polya sites +.LEFT MARGIN2 +.para +Simply searches for the sequence AATAAA + (Proudfoot and Brownlee Nature 263, 211-214, + 1982) and marks it with a short vertical line. +.left margin1 +@66. TX 1 @ Interconvert t and u +.LEFT MARGIN2 +.para +This function interconverts T and U characters in the active sequence i.e +between DNA and RNA. +.LEFT MARGIN1 +@67. TX 7 @ Search for patterns of motifs +.left margin2 +.para +This option searches for patterns of motifs. Patterns can be defined +interactively or read from files. Results can be displayed in several ways +in both graphical and textual form. Used to create pattern files for +searching libraries. The option is extremely flexible and consequently the +following documentation is quite lengthy. However the routine is capable +of searching for almost any known pattern. In addition the flexibility +does not necessitate difficulty of use, and the userinterface has been +simplified considerably since the methods were first published. +.para +Users should refer to the "typical dialogue" shown below for the most +helpful information on using the program. +.para +There are currently +four ways to display the matching patterns: 1=each individual +motif and its position is listed; 2=all the sequence between, and +including the two +outermost motifs is listed; 3=graphical, with a vertical line marking the +position +of the leftmost motif; 4 = EMBL feature table format, where the KEYNAM +field if the motif name, the FROM and TO fields denote the ends of the +match, and the DESCRIPTION field is "Program". +.para +When it is defined for the first time a pattern must be entered +interactively at the keyboard, but the pattern description +can be saved to a file. +This file can be used for all subsequent searches. +.para +When defining a pattern interactively +select a motif class and the program will request the required inputs. +.para +The program gives each motif an identifying name and number. +For motifs other than the first, a range of allowed positions must be +defined (Note that sets of motifs included using the OR operator will all +be given the same range, and so the program will only request range +values +for the first motif in any such set). +To specify the allowed range for a motif the user must supply the +following: the +identifying number of the motif, relative to which the current motifs +positions are to be defined (termed the "reference motif"); a "relative start +position" and a range. The relative start position can be negative or positive. +A negative start position means that although the reference motif +is searched for first, the current motif can be found to its left. +A zero relative start position means their left ends are superimposed. The +default start position is to butt-joint the motif to righthand end of the +"reference motif". The range is "the number of extra positions" that the +motif can take. +.para +The program will display the probability of finding each motif. These +values are presented in the following form: .1234E-5 means 0.1234 times +10 +to the power -5. +.para +After the pattern has been defined, the program will type a description +of +it on the screen. It will then allow the user to give an overall cutoff +score and overall probability cutoff. +.para +Typical dialogue for all the different motif classes is displayed below. +.lit + +? Menu or option number=67 + Pattern searcher +? (y/n) (y) Read pattern from keyboard +X 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Complement of weight matrix + 6 Inverted repeat or stem-loop + 7 Exact match, defined step + 8 Direct repeat + 9 Pattern complete +? 0,1,2,3,4,5,6,7,8,9 = +? Motif name=Ematch +? String=AA +Probability of score 2.0000 = 0.595E-01 +X 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Complement of weight matrix + 6 Inverted repeat or stem-loop + 7 Exact match, defined step + 8 Direct repeat + 9 Pattern complete +? 0,1,2,3,4,5,6,7,8,9 =2 +? Motif name=AAA +X 1 And + 2 Or + 3 Not +? 0,1,2,3 = +? Number of reference motif (1-1) (1) = +? Relative start position (-1000-1000) (3) = +? Number of extra positions (0-1000) (0) = +? string=AAA +? Minimum matches (1.00-3.00) (3.00) =2 +Probability of score 2.0000 = 0.149E+00 + 1 Exact match +X 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Complement of weight matrix + 6 Inverted repeat or stem-loop + 7 Exact match, defined step + 8 Direct repeat + 9 Pattern complete +? 0,1,2,3,4,5,6,7,8,9 =3 +? Motif name=T'S +X 1 And + 2 Or + 3 Not +? 0,1,2,3 = +? Number of reference motif (1-2) (2) = +? Relative start position (-1000-1000) (4) = +? Number of extra positions (0-1000) (0) = +? String=TTT +? Minimum score (0.00-108.00) (108.00) =72 +Probability of score 72.0000 = 0.258E+00 + 1 Exact match + 2 Percentage match +X 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Complement of weight matrix + 6 Inverted repeat or stem-loop + 7 Exact match, defined step + 8 Direct repeat + 9 Pattern complete +? 0,1,2,3,4,5,6,7,8,9 =4 +? Motif name=GCN4 +X 1 And + 2 Or + 3 Not +? 0,1,2,3 = +? Number of reference motif (1-3) (3) = +? Relative start position (-1000-1000) (4) = +? Number of extra positions (0-1000) (0) = +? Weight matrix file name=GCN4 + GCN4 FROM WEIGHTS 17-11-87 +Probability of score -22.0020 = 0.139E-02 + 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix +X 4 Cut-off score and weight matrix + 5 Complement of weight matrix + 6 Inverted repeat or stem-loop + 7 Exact match, defined step + 8 Direct repeat + 9 Pattern complete +? 0,1,2,3,4,5,6,7,8,9 =5 +? Motif name=GCN4 +X 1 And + 2 Or + 3 Not +? 0,1,2,3 = +? Number of reference motif (1-4) (4) = +? Relative start position (-1000-1000) (20) = +? Number of extra positions (0-1000) (0) = +? Weight matrix file name=GCN4 + GCN4 FROM WEIGHTS 17-11-87 +Probability of score -22.0020 = 0.606E-03 + 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix +X 5 Complement of weight matrix + 6 Inverted repeat or stem-loop + 7 Exact match, defined step + 8 Direct repeat + 9 Pattern complete +? 0,1,2,3,4,5,6,7,8,9 =6 +? Motif name=LOOP +X 1 And + 2 Or + 3 Not +? 0,1,2,3 = +? Number of reference motif (1-5) (5) = +? Relative start position (-1000-1000) (20) = +? Number of extra positions (0-1000) (0) = +? Stem length (1-60) (6) = +? Minimum loop length (-6-60) (0) = +? Maximum loop length (0-60) (0) =5 +? Minimum score (1.00-12.00) (12.00) =10 +Probability of score 10.0000 = 0.598E-02 + 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Complement of weight matrix +X 6 Inverted repeat or stem-loop + 7 Exact match, defined step + 8 Direct repeat + 9 Pattern complete +? 0,1,2,3,4,5,6,7,8,9 =7 +? Motif name=Tstep +X 1 And + 2 Or + 3 Not +? 0,1,2,3 = +? Number of reference motif (1-6) (6) = +? (y/n) (y) Relative to 5 prime end +? Relative start position (-1000-1000) (1) = +? Number of extra positions (0-1000) (0) = +? String=TTT +? Step (1-20) (3) = +Probability of score 3.0000 = 0.367E-01 + 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Complement of weight matrix + 6 Inverted repeat or stem-loop +X 7 Exact match, defined step + 8 Direct repeat + 9 Pattern complete +? 0,1,2,3,4,5,6,7,8,9 =8 +? Motif name=REPEAT +X 1 And + 2 Or + 3 Not +? 0,1,2,3 = +? Number of reference motif (1-7) (7) = +? Relative start position (-1000-1000) (4) = +? Number of extra positions (0-1000) (0) =2 +? Repeat length (1-60) (6) = +? Minimum gap (0-60) (0) = +? Maximum gap (0-60) (0) =4 +? Minimum score (1.00-6.00) (6.00) =5 +Probability of score 5.0000 = 0.554E-02 + 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Complement of weight matrix + 6 Inverted repeat or stem-loop + 7 Exact match, defined step +X 8 Direct repeat + 9 Pattern complete +? 0,1,2,3,4,5,6,7,8,9 =9 +? (y/n) (y) Save pattern in a file N + +Pattern description + +Motif 1 named Ematch is of class 1 +Which is an exact match to the string +AA +Motif 2 named AAA is of class 2 +which is a match of score 2. to the string +AAA +and the 5 prime base can take positions 3 to 3 +relative to the 5 prime end of motif 1 +It is anded with the previous motif. +Motif 3 named T'S is of class 3 +which is a match of score 72. to the string +TTT +and the 5 prime base can take positions 4 to 4 +relative to the 5 prime end of motif 2 +It is anded with the previous motif. +Motif 4 named GCN4 is of class 4 +Which is a match to a weight matrix with score -22.002 +and the 5 prime base can take positions 4 to 4 +relative to the 5 prime end of motif 3 +It is anded with the previous motif. +Motif 5 named GCN4 is of class 5 +Which is a match to the complement of a weight matrix with score -22.002 +and the 5 prime base can take positions 20 to 20 +relative to the 5 prime end of motif 4 +It is anded with the previous motif. +Motif 6 named LOOP is of class 6 +Which is a stem-loop structure with stem length 6 and score 10. +The loop can have sizes 0 to 5 +and the 5 prime base can take positions 20 to 20 +relative to the 5 prime end of motif 5 +It is anded with the previous motif. +Motif 7 named Tstep is of class 7 +Which is an exact match to the string +TTT +with a step size of 3 +and the 5 prime base can take positions 1 to 1 +relative to the 5 prime end of motif 6 +It is anded with the previous motif. +Motif 8 named REPEAT is of class 8 +Which is a repeat with repeat length 6 and score 5. +The loop-out can have sizes 0 to 4 +and the 5 prime base can take positions 4 to 6 +relative to the 5 prime end of motif 7 +It is anded with the previous motif. +Probability of finding pattern = 0.2348E-14 +Expected number of matches = 0.5100E-09 +? Maximum pattern probability (0.00-1.00) (1.00) = +? Minimum pattern score (-9999.00-9999.00) (-9999.00) = + Select display mode +X 1 Motif by motif + 2 Inclusive + 3 Graphical + 4 EMBL feature table +? 0,1,2,3,4 =4 + Searching + + +Total matches found 0 + +Menus and their numbers are +m0 = This menu +m1 = General +m2 = Screen control +m3 = Statistical analysis of content +m4 = Structures and repeats +m5 = Translation and codons +m6 = Gene search by content +m7 = Prokaryotic signal search +m8 = Eukaryotic signal search + ? = Help + ! = Quit +? Menu or option number=67 + Pattern searcher +? (y/n) (y) Read pattern from keyboard +X 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Complement of weight matrix + 6 Inverted repeat or stem-loop + 7 Exact match, defined step + 8 Direct repeat + 9 Pattern complete +? 0,1,2,3,4,5,6,7,8,9 = +? Motif name=Arun +? String=AAAAAA +Probability of score 6.0000 = 0.210E-03 +X 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Complement of weight matrix + 6 Inverted repeat or stem-loop + 7 Exact match, defined step + 8 Direct repeat + 9 Pattern complete +? 0,1,2,3,4,5,6,7,8,9 =9 +? (y/n) (y) Save pattern in a file N + +Pattern description + +Motif 1 named Arun is of class 1 +Which is an exact match to the string +AAAAAA +Probability of finding pattern = 0.2103E-03 +Expected number of matches = 0.1522E+01 +? Maximum pattern probability (0.00-1.00) (1.00) = +? Minimum pattern score (-9999.00-9999.00) (-9999.00) = + Select display mode +X 1 Motif by motif + 2 Inclusive + 3 Graphical + 4 EMBL feature table +? 0,1,2,3,4 =4 + Searching + + +FT Arun 1582 1587 Program +FT Arun 3160 3165 Program +FT Arun 4204 4209 Program +FT Arun 5691 5696 Program +FT Arun 6710 6715 Program +Total matches found 5 +Minimum and maximum observed scores 6.00 6.00 + +.end lit +.para +These methods allow users to define and search for +complex patterns of motifs defined as single objects. +The programs allow individual DNA motifs to be defined in eight +different +ways, and protein motifs in six. Motifs are combined, using the logical +operators AND, OR and NOT, to describe a pattern. The pattern also +specifies the ranges of allowed relative separations of the individual +motifs. +.para +First some definitions. +.para +A MOTIF is a contiguous subsequence of fixed length. +At its simplest +it could be a single definite base or amino acid; a more complex motif +might be better represented as a consensus or a weight matrix; +two more-abstract types of +motif are direct and inverted repeats. +.para +A PATTERN is a higher order of structure defined by a list of motifs. The +motifs in a pattern are combined using the logical operators AND, OR and +NOT. The list also defines the allowed relative separations of the +motifs. In the current versions of the programs up + to 50 motifs can be combined into a single pattern. So using these +definitions there are two +differences between motifs and patterns: 1) the distances between all +elements of a motif are fixed, but +the separations of parts of patterns can vary; + 2) all characters in a motif are defined +using the same method (class), but different parts of a pattern can be +defined in completely different ways. +.para +Each motif +can be represented in 9 ways (known as the motif class): +.sk1 +.lit + MOTIF CLASSES +CLASS DESCRIPTION + 1 Exact match to a short defined sequence. The IUB symbols + can be used for DNA sequences. + 2 Percentage match to a defined short sequence. In nucleic acids, + the IUB symbols can be used. + 3 Match to a defined sequence, using a score matrix and cutoff + score. The DNA matrix (see option 18) gives scores to IUB symbols + depending on their level of redundancy. MDM78 is used for proteins. + 4 Match to a weight matrix with cutoff score. + 5 As class 4 but on the complementary strand. + 6 Inverted repeat or stem-loop. Fixed stem length, range of + loop sizes, and cutoff score using A-T, G-C=2; G-T=1. + 7 Exact match to short sequence but with a defined step size. + 8 Direct repeat. Fixed repeat length, range of loop-out sizes, + cutoff score, and score matrix (for protein sequences MDM78 and + for nucleic acids an identity matrix). + 9 Membership of a set. A list of sets of allowed amino acids for + each position in the motif. The sets are separated by commas(,). + For example IVL,,,DEKR,FYWILVM defines a motif of length 5 amino + acids in which one of I,V or L must be found in the first position, + then anything in the next two positions, D,E,K or R in the fourth + position and F,Y,W,I,L,V or M in the fifth. This class only applies + to protein sequences because for nucleic acids "membership of a +set" + can be achieved using IUB symbols. + + Classes 1 - 4, 8 and 9 apply to protein sequences, and classes 1-8 to + nucleic acids. + +.end lit +.para +Class 1: exact match. +.para +The motif is defined by a short sequence, which for nucleic acids, + may include IUB symbols. All symbols must match. +.para +Class 2: percentage match +.para +The motif is defined by a short sequence, which for nucleic acids, +may include IUB symbols. The minimum number of matching characters +must +also be specified. +.para +Class 3: match using a score matrix +.para +The motif is defined by a short sequence, which for nucleic acids, +may include IUB symbols. The motif is not compared directly with the +sequence to count the number of matching characters. Instead a matrix is +used to provide a score for all possible pairs of characters. The motif +score for +any position along the sequence is the sum of the scores found by +looking-up the scores for each pair of aligned characters. A match is +declared if some minimum score is achieved. +.para +Class 4: weight matrix +.para +The motif is defined by a table of values (called weights or scores). The +table gives a score for finding each possible character at each position +along the length of the motif. It therefore +has dimension motif-length x character-set-size, and allows us to give +different scores for each character at each position. It is equivalent to +having a different score matrix for each position along the motif, and +provides the most flexible and specific method of defining motifs. The +weight matrices are created by program NIP option 20 and +stored as files. The file contains the values +for each position, as well as an overall minimum score. +There are two ways in which these values can be used to calculate an +overall +score for any section of the sequence. The simplest way is to add the +values in the file. (This means that the highest possible score +can be calculated by adding the top value at each column +position, and the lowest +by adding the bottom value.) + The normal way of using the values in the file is as +follows. +First the programs divide the values in each column by the column total +so +that they sum to 1.0 +Then the natural +logs of these values are used as scores. When the matrix is applied to a +sequence these logarithmic values are summed (which is of course +equivalent +to multiplying the frequencies). +Note that using the natural logs of the frequencies as +weights and +adding them means that the overall cutoff score must be less than zero, +whereas if the original +values in the weight matrix file are added, the cutoff score will be +greater than zero. The search routines therefore decide whether the user +wants to add values or multiply frequencies +by examining the value of the cutoff score: it will add if the cutoff +is +greater than zero and add logs of frequencies if it is less than zero. + Hence we effectively get two +motif classes in one. The program NIP, when creating weight matrix +files, will ask the user whether the scores should be added or multiplied. + If the values in the table have been defined +without using a set of aligned sequences +it is easier for the user to +choose a cutoff score if the values are added. +.para +Class 5: complement of weight matrix +.para +The motif is defined by a weight matrix, but the program searches for its +complement. +.para +Class 6: inverted repeat, or stem-loop +.para +The motif is defined by a repeat length, a minimum score + and a range of loop sizes. The scores are A-T=2, G-C=2, G-T=1, else=0. +The loop sizes are defined by a minimum +and maximum distance from the 3' end of the stem. +For a stem-loop these will be positive numbers. For example to +define a stem of length 8 and loop sizes varying from 3 to 5, the stem +would be set to 8, the minimum start distance to 3 and the maximum +to 5. To define an +inverted repeat the minimum distance will be negative. For example stem +length=9, +minimum distance=-9, and maximum distance=-8 will find +inverted repeats of lengths 9 and 10. +E.g. AAAAATTTT and AAAAATTTTT would be found, the first having a base +at +its centre, the second having none. +.para +Class 7: exact match, defined step size. +.para +The motif is defined by a short sequence, which for nucleic acids, + may include IUB symbols. All symbols must match. The class differs +from +class 1 in that searches will move in steps of some given size. For +example +we could search for a certain codon and use a step size of 3 and hence + keep in a +single reading frame. +.para +Class 8: direct repeat +.para +The motif is defined by a repeat length, a minimum score + and a range of loop sizes. The scores are defined using MDM78 for protein +sequences and an identity matrix for nucleic acids. +The loop sizes are defined by a minimum +and maximum distance from the 3' end of the stem. +.para +Class 9: membership of a set +.para +This motif class is for protein sequences. It is defined by lists of +allowed amino acids for each position in the motif, and a cut-off score. +Positions at which any amino acid can occur are left blank. +All allowed amino acids for each position give a score of 1. +The motifs can be defined in two ways: either typed at the keyboard or +read +in as a weight-matrix-like file. +When the motif is defined at the keyboard the sets of allowed amino +acids +are separated by commas(,). + For example IVL,,,DEKR,FYWILVM defines a motif of length 5 amino + acids in which one of I,V or L must be found in the first position, + then anything in the next two positions, D,E,K or R in the fourth + position and F,Y,W,I,L,V or M in the fifth. To specify that the +whole motif must match a score of 3 would be required (i.e. one of the +allowed amino acids must be found for each of the three defined +positions). +If the motif is read from a file the file must have been written by +program +NIP, or have been saved by the pattern searching routines. If the +user +elects to save a pattern, and it includes class 9 motifs typed at the +keyboard, then the program will save the class 9 motifs as weight matrix +files. Therefore it will request file names for each motif of this class. +If the motif given above as an example were saved the weight matrix file +would have 5 columns. +The first column +would contain zeroes except for the I, V and L rows +which would be set to 1; the next two columns would all be zero; the next +would be zero except for the D,E,K and R rows which would be 1; the final +column would contain 1's in rows F,Y,W,I,L,V and M, with +the rest zero. +.para + +The logical operator (AND, OR or NOT) used to add each motif to the +pattern +is specified by preceding +the class number by the letters A, O or N. A = AND, O = OR, N = NOT. +The default is A, so N2 means include, using the NOT operator, a class 2 +motif; O2 means include, using the OR operator, a class 2 motif; both A2 +and +2 mean include, using the AND operator, a class 2 motif. + +.para +Range setting. +.para +The motifs in a pattern are numbered according to their order in the list. +Apart from the first motif in a pattern all motifs are given a range +of allowed positions relative to a motif further up the list. +For example +suppose we have a pattern defined by A AND B AND C AND D. +Motif A can occur anywhere, but B must have its range of allowed +positions defined relative to the position of motif A, and C's positions +can be defined relative to either A or B, depending on which is most +convenient, and likewise D's positions can be relative to A or B or C. +.para +Notice that the positions of motifs can be defined relative to more than +one motif. Suppose we have a pattern consisting of +motifs A, B and C, and that B occurs 5-10 residues right of A, C occurs 5- +10 +residues right of B, and also C is never more than 15 residues from A. +Then +it is quite consistent with the methods to include motif C into the +pattern +twice using the AND operator: once relative to A and once relative to B. +This will define the relative spacing and the ORDER of the motifs in the +pattern. (If we simply defined the position of C relative to A it could be +found to the left of B). +.para +Motifs combined together using the OR operator are all given the same +range. For example suppose we had a pattern A AND (B OR C) AND (D OR E), + then B and C each have the same range, and D and E also have +the same range as one another. The range for D and E can be relative to +A or to B. +.para +Motifs cannot have their ranges defined relative to motifs that are +included using the NOT operator. For example if we had the pattern A NOT +B +AND C, then the range for C can only be defined relative to motif A. +.para +Speed can be gained by arranging the order +of the motifs so that those higher up the list are of types that can be +searched for rapidly and that are also unlikely to be found. +.para +Motifs combined by the OR operator are alternatives: if any one of a set +of motifs +combined by the OR operator is found, then a match is declared. All +alternatives will be reported. For example if we had a pattern defined by +A +AND (B OR C), then all places where A occurs and B is found within range, +and all places where A is found and C is found within range will be +reported. A typical use would be where we might allow a motif to appear +on +either strand of the DNA sequence. For example a weight matrix +representing +the heatshock element could be used in a pattern which included +heatshock +as a motif class 4 combined using the OR operator +with heatshock as a motif class 5. +.para +The probability calculations are performed for each motif as it is +defined. +If an overall probability cut-off is given the calculation is repeated for +each match found. To achieve maximum searching speed do not give an +overall +probability cut-off. Overall cut-off scores should only be used if the +motif +classes used are compatible. +.para +There are currently +several ways to display the matches: 1 = each +motif and its position is listed; 2 = all the sequence between the two +outermost motifs is listed; 3 = graphical, with a spike marking the +position +of the leftmost motif. The library versions also give entry names, and a +one +line title; in addition they can be used to produce aligned families of +sequences. When this mode of output is selected the program will write a +separate file for each match. The files will be called ENTRYNAME.DAT +where +ENTRYNAME is the name of the entry in the library. The matching +sequence +will be written out so that the spacing between motifs is constant, and +set to the maximum allowed by the pattern definition. Any gaps will be +filled with dashes (-). If the individual sequences were subsequently +written one above the other +they should line up so that all motifs are in register. There two types of +output of this sort: one, option 4, writes out whole sequences, the other, +option 5, writes out only the sequences between the two outermost +motifs. +If the individual sequences were subsequently +written one above the other +they should line up so that all motifs are in register. There two types of +output of this sort: one, option 4, writes out whole sequences, the other, +option 5, writes out only the sequences between the two outermost +motifs. +Note that for option 4 users are asked to type the position of the +first motif, and the reason for +this is explained below. +Consider a pattern found in several sequences. Consider only +the first motif in +the pattern and suppose that it was found in different positions in these +sequences. +Say that of these positions the one furthest from the left end was +position 100. Then, in order to ensure that all the sequences would align, +we must specify that motif 1 must start at position 100. +Any sequences in which motif 1 started +nearer to the left end than position 100 would be padded accordingly. +These modes of output +should only be used when the position of each motif is defined relative to +its +immediate neighbour. +.para +The pattern descriptions can be saved to files. These files +can be used instead of typing definitions again at the keyboard. As the +files are annotated, +they can easily +be changed using system editors, and the modified versions used to +define the variant patterns for the programs. +.para +Use of lists of entry names +.para +The two programs that operate on libraries have the ability to +restrict their searches to subsets of the libraries. This does not require +sublibraries to be created but instead is achieved by using files +containing a list of the entry names of sequences. The user may choose to +search only those entries on the list or, alternatively to search all but +those on the list (i.e. in the latter case +the list contains the names of those to be excluded). + The programs can search libraries that have indexes and those that +do not. + If a list of names for inclusion is used, +then the search will be faster if the index is present. In all other +circumstances the whole library will be read. +The list must be in library order except when it is used +to include entries, and an index is available. +The list must contain each entry name on a separate line, with the name +starting in column 1 of the line. ie there must be no spaces at the start +of the line. +The list of entry names +can be produced by the keyword searches of nip, pip, etc as long +as the listings produced have a space character separating the entry name +from the entry description. This will depend on how well the library +reformatting programs work. For example swissprot entry names tend to run +into the beginning of the descriptions, but other libraries are generally +OK. +.para +One use of the programs is to look for patterns that we already know +about, but in new sequences. However it is hoped that they will also be +useful for finding new motifs. For example +several known control regions in +nucleic acid +sequences consist of particular direct or inverted repeats; +the inclusion of +direct and inverted repeats as motif classes +makes it possible to +find previously unknown +motifs of these types. +Using these new programs we can +ask questions like: "are there any inverted or direct repeats near to +sections of sequence that contain both a +CCAAT box and a TATA box?"; and to search for such things throughout +the +libraries. In addition, the mode of output in which all the sequence +between +the two outermost motifs found is printed out, allows us to extract +sequences and examine them in more detail for further common +subsequences. +For example we might want to collect together all the sequences +between +putative CCAAT and TATA boxes. +.para +A further use of the inverted repeat motif class is the following. If a +regulatory sequence in DNA is poorly defined but also an inverted repeat, +then it might be an advantage to specify it both as a consensus sequence +and +a superimposed inverted repeat. In this way two weak definitions can be +combined to produce a stronger pattern. +.para +Given only a few examples of a motif it +should be possible to perform initial searches using a +class 3 motif, and then, using plausible matching sequences, create a +more +specific weight matrix for the same motif. +.para +If motifs are combined with the first motif using the OR operator +they will be ignored until all +permutations that include the first motif have been looked for. +The whole search will then be repeated, in +turn, for each of +those motifs that are combined with the first motif using the OR +operator. +An interesting consequence of this is that the program can be used, +without +change, to compare any newly determined sequence with all known +individual +motifs. We achieve this by having a pattern in which all known relevant +motifs are combined using the OR operator. +If we ask to use this pattern with +a sequence, the program will automatically compare each individual +motif in +the pattern with the whole length of the +sequence. As the number of known +motifs grows this should become an increasingly useful standard +procedure. +.para +The NOT operator is obviously +useful for making sure particular motifs are not present, but it can also +be used to bracket the levels of matches found. We may want a degree of +match that lies between two limits - binding should occur, but not too +strongly; or base-pairs should form, but not too many. We can specify +this +by asking for a match with a low score, in combination with a match and +a +high score, both for the same motif, but with the high score included +using +the NOT operator. +.para +The algorithm is designed to find all sections of a sequence that satisfy +the pattern rather than only the best match. +Particularly if some of the motifs in a pattern are less well defined than +others, this can often result in the same region of a sequence being +reported as having several matches, but which only vary in the +positions of the weakest motifs. +.para +General remarks on motif searching +.para +Generally motifs are short subsequences that are thought to be +associated with +particular functions in some known sequences. Often +we search for them to try to +understand or interpret other sequences. Sometimes we search for +motifs and +patterns to +test a hypothesis about their role: are they found in the expected +positions in the expected sequences. In doing so we should remember +that, in both proteins and nucleic acids, + what we are really looking for is a particular +three dimensional structure with certain affinities for other structures, +and that we are assuming that the sequence of the motif alone +defines the 3D structure we searching for. + The overall structure +may be completely different to those in which the motif is functional, +and +hence the motif may have a different shape or be inaccessible. +We should be aware of the +importance of the context in which a motif is found. Where does it lie +relative to the overall structure, is it accessible, is the three +dimensional spacing between +it and other motifs correct? For example, is it on the same side of the +double helix, and the correct distance from some other motif? How does +context affect our assessment of the significance of finding a motif? +Finding false mammalian mRNA splice junctions in non-coding sequences +is +far less important than finding false sites in pre-mRNA sequences, but +finding them in the correct places is most important! In other words, it +is +often the case that when we are searching for a motif that is known to +be +necessary for some function, then a positive result in the form of a +match +in the required position, is more important than a high background of +matches in the wrong positions. Being + able to write +down the probability of finding a motif in a random sequence tells us how +well it is defined. +In nucleic +acids the DNA may contain many superimposed types of information such +as +those concerned with histone phasing, protein coding or mRNA secondary +structure. These overlapping "codes" may interfere with one another +causing +matches to motifs to be poorer than expected. +In general we will only have a limited number of examples of the +motif and we do not know how representative they are. +.para +Sequences have superimposed functions: some parts may be of general +structural +importance and give rise to an overall framework, and other parts give +specificity and hence are not common; we may want to use a set of +aligned +sequences to define a motif, but want to use only the framework +positions. + Alternatively we may want to pick out +only those parts of a set of aligned sequences that give a particular +property, and to ignore other similarities that are due to some other +property +and which could obscure the pattern +we are interested in. +It is possible to apply a mask to a set of aligned sequences in +order to give weight to selected positions only. + The ability to define a mask allows certain positions +to be used in the motif and others to be ignored, and yet still permits the +use of a set of aligned sequences to calculate weights. The mask is +requested and applied +by the program and results in the masked positions being zero +in +the weight matrix. The mask is defined in the following way. +Suppose we had a motif of length 15, then the mask +x--x--xx-x will give zero weights to positions 2,3,5,6 and 9 (note it is +the dashes (-) that are significant and that positions +1,4,7,8,10,11,12,13,14 and 15 +will be non-zero). Of course +the same set of sequences could be used with several alternative masks +in +order to extract different features and create corresponding weight +matrices. +.para +The programs are described in Staden,R. +CABIOS 4, 53-60, 1988; Staden,R. + CABIOS 5, 89-96, 1989, and Methods in Enzymology 183, 193-211 (1990). +.left margin1 +@ end of help diff --git a/help/NIPF.RNO b/help/NIPF.RNO new file mode 100644 index 0000000..ccc4bad --- /dev/null +++ b/help/NIPF.RNO @@ -0,0 +1,88 @@ +.NPA +.SP 1 +.left margin1 +@-1. TX 0 @General +.sp +@-2. TX 0 @Screen control +.sp +@-3. TX 0 @Statistical analysis +.sp +@-1. TX 0 @General +.sp +@-2. TX 0 @Screen control +.sp +@-3. TX 0 @Statistical analysis +.sp +@0. TX -1 @NIPF +.sp +@1. TX 1 @ Help +.sp +@2. TX 1 @ Quit +.sp +@3. TX 1 @ Read new sequence +.sp +@4. TX 1 @ Redefine active region +.sp +@5. TX 1 @ List the sequence +.sp +@6. TX 1 @ List a text file +.sp +@7. TX 1 @ Direct output to disk +.sp +@8. TX 1 @ Write active sequence to disk +.sp +@9. TX 1 @ List a translation +.sp +@32. TX 1 @ List showing base differences +.sp +@37. TX 1 @ List showing translation +.sp +@33. TX 1 @ List showing amino acid differences +.sp +@10. TX 2 @ Clear graphics +.sp +@11. TX 2 @ Clear text +.sp +@12. TX 2 @ Draw a ruler +.sp +@13. TX 2 @ Use cross hair +.sp +@14. TX 2 @ Reset margins +.sp +@15. TX 2 @ Label diagram +.sp +@16. TX 2 @ Display a map +.sp +@17. TX 3 @ Set comparison mode +.sp +@18. TX 3 @ Set sort mode +.sp +@21. TX 3 @ Count base changes +.sp +@22. TX 3 @ Count codon changes +.sp +@23. TX 3 @ Count genetic events +.sp +@24. TX 3 @ Show table of base changes +.sp +@36. TX 3 @ Show table of expressed base changes +.sp +@39. TX 3 @ Show table of silent base changes +.sp +@38. TX 3 @ Estimate mutation rate +.sp +@25. TX 3 @ Plot base changes +.sp +@26. TX 3 @ Plot expressed changes per base +.sp +@27. TX 3 @ Plot silent changes per base +.sp +@28. TX 3 @ Count expressed changes per base +.sp +@29. TX 3 @ Count silent changes per base +.sp +@30. TX 3 @ Count changed amino acids +.sp +@31. TX 3 @ Plot amino acid variability +.sp +@ end of help diff --git a/help/PIP.RNO b/help/PIP.RNO new file mode 100644 index 0000000..24e42a2 --- /dev/null +++ b/help/PIP.RNO @@ -0,0 +1,2469 @@ +.NPA +.SP 1 +.left margin1 +@-1. TX 0 @General +.sp +@-2. T 0 @Screen control +.sp +@-2. X 0 @Screen +.sp +@-3. T 0 @Statistical analysis of content +.sp +@-3. X 0 @Statistics +.sp +@-4. T 0 @Structures and repeats +.sp +@-4. X 0 @Structures +.sp +@-5. TX 0 @Search +.sp +@0. TX -1 @PIP +.para +This is a program for analysing individual protein sequences. It can read +sequences stored in many of the most commonly used formats, and +performs all of the usual simple analyses. In addition it has very flexible +search procedures and presents many of its results graphically. +.PARA +The following analyses (preceded by their option numbers) are included: +.lit + ? = Help + ! = Quit + 3 = read a new sequence + 4 = define active region + 5 = list the sequence + 6 = list a text file + 7 = direct output to disk + 8 = write active sequence to disk + 9 = edit the sequence +10 = clear graphics screen +11 = clear text screen +12 = draw a ruler +13 = use cross hair +14 = reposition plots +15 = label diagram +16 = display a map +17 = search for short sequences +18 = compare a sequence +19 = compare a sequence using a score matrix +20 = search for a sequence using a weight matrix +21 = calculate amino acid composition +22 = plot hydrophobicity +23 = plot charge +24 = plot Robson prediction +25 = plot hydrophobic moment +26 = draw helix wheel +27 = back translate +28 = search for patterns of motifs +.end lit +.para +Some of these methods produce graphical + results +and so the +program is generally used from a graphics terminal (a vdu on which lines +and points can be drawn as well as characters). +.para +For users of VT640's or their equivalents the +terminal must be set nowrap (type NOWRAP) prior to running the program. +.LEFT MARGIN2 +The positions of each of the plots is defined relative to a users drawing +board which has size 1-10,000 in x and 1-10,000 in y. +Plots for +each option are drawn in a window defined by x0,y0 and xlength,ylength. +Where x0,y0 is the position of the bottom left hand corner of the window, + and xlength is the width of the window and ylength the +height of the window. +.lit + --------------------------------------------------------- 10,000 + 1 1 + 1 -------------------------------------- ^ 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 1 1 ylength 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 -------------------------------------- v 1 + 1 x0,y0^ 1 + 1 <---------------xlength--------------> 1 + --------------------------------------------------------- 1 + 1 10,000 + +.end lit +All values are in drawing board units (i.e. 1-10,000, 1-10,000). +The default window positions are read from a file "ANALPMRG" when the +program is started. Users can have their own file if required. +.para +The program can handle sequences stored in several formats: +Staden, EMBL, GENBANK, PIR (also known as NBRF) and GCG and they are described +in +the help for 'READ NEW SEQUENCE'. +.para +The options for the program are accessed from 5 main menus: general, +screen control, statistical analysis of content, structure, search. +Both menus and options are selected by number. +.LEFT MARGIN1 +@1. TX 0 @Help +.LEFT MARGIN2 +.para +This option gives online help. The user should select option numbers and +the current documentation will be given. Note that option 0 gives an +introduction to the program, and that ? will get help from anywhere in +the +program. +The following analyses (preceded by their option numbers) are included: +.sp +.left margin1 +@2. TX 0 @Quit +.left margin2 +.para +This function stops the program. +.left margin1 +@3. TX 1 @Read a new sequence +.LEFT MARGIN2 +.para +This option allows users to read in new sequences, browse through annotations, + or search sequence +libraries for keywords. Sequences can be read from "personal" +sequence files or from sequence libraries. These are referred to as the +sequence "source". Personal files can be stored in several formats: +Staden, PIR, EMBL, GENBANK and GCG. +At LMB we use "Staden" format for sequencing and all +the +libraries are stored in their original formats. Note, however, that libraries +such as EMBL or GenBank that are divided into several files (eg GenBank has +13 separate files) are indexed as a whole. This means that users do not need +to know which file contains an entry, only which library. +When the user selects to read in a sequence the program first asks for the +sequence "source". +.para +If the user selects "personal" the program will ask for +the format (Staden, PIR, EMBL, GENBANK or GCG), and then for the name of +the file. For PIR format the user will also be required to know the entry +name of the sequence as the file can contain several. For the other formats +only a single entry is expected. The file will be read, its length and +composition will be displayed and the option left. +.para +If the user selects "library" as the sequence source the program will display a +list of available libraries. The programs are capable of handling all current +libraries but which ones are available will vary from site to site. At LMB we +have several libraries and also weekly updates of data gathered between releases. +The program will ask users to select a library and then give a list of options: +.lit + + X 1 Get a sequence + 2 Get annotations + 3 Get entrynames from accession numbers + 4 Search titles for keywords + 5 Search text index for keywords + +.end lit +If get a sequence or get annotations is selected users will be asked to +type the entry name. The option will be left when a sequence is selected or +! is typed. The composition and length will be displayed. +.para +The text index contains all words from feature tables, reference titles, +definition lines, keywords lists and comments, so the text index search +is most useful. It is also the fastest. Up to 5 words can be searched for +at once. The words should be typed separated by spaces, for example +.lit + ? Keywords=P53 mouse murine tumo + +.end lit +will search for all entries that contain words starting with p53, mouse, +murine and tumo. Only the unique entries that contain ALL words will be +listed. Before listing the matching entries +the program will show the number of 'hits' for each word and ring the bell. +Escape is possible at this point, or after each screenfull of entries. +In addition to the entry names the text search displays the primary accession +number, the sequence length and up to 80 characters of description. +(The search of 'titles' is now redundant because the full text index +contains all the title words and the search is much faster. It will probably +be removed from the program.) +All searches are independent of case. Where +possible the program will offer default entry names. +.para +Typical dialogue follows. +.lit +Select sequence source +X 1 Personal file + 2 Sequence library +? Selection (1-2) (1) = +Select sequence file format +X 1 Staden + 2 EMBL + 3 GenBank + 4 PIR + 5 GCG +? Selection (1-5) (1) = +? Sequence file name=M13MP7.SEQ + Contig title removed +Sequence length= 7238 + Sequence composition + T C A G - + 2405. 1539. 1765. 1527. 2. + 33.2% 21.3% 24.4% 21.1% 0.0% + . + . + . + + + Select sequence source + X 1 Personal file + 2 Sequence library + ? Selection (1-2) (1) =2 + Select a library + X 1 EMBL 29 nucleotide library Dec 91 + 2 SWISSPROT 20 protein library Nov 91 + 3 PIR 31 protein library Dec 91 + 4 NRL3D 58 From Brookhaven protein library Dec 91 + 5 GenBank + ? Selection (1-5) (1) = +Library is in EMBL format with indexes + Select a task + X 1 Get a sequence + 2 Get annotations + 3 Get entry names from accession numbers + 4 Search titles for keywords + 5 Search text index for keywords + ? Selection (1-5) (1) =5 + Search for keywords + ? Keywords=P53 mouse +P53 hits 68 +MOUSE hits 8180 + + MMANT01 X00875 536 Murine gene fragment for cellular tumour antigen + MMANT02 X00876 83 Murine gene fragment for cellular tumour antigen + MMANT03 X00877 21 Murine gene fragment for cellular tumour antigen + MMANT04 X00878 261 Murine gene fragment for cellular tumour antigen + MMANT05 X00879 184 Murine gene fragment for cellular tumour antigen + MMANT06 X00880 113 Murine gene fragment for cellular tumour antigen + MMANT07 X00881 110 Murine gene fragment for cellular tumour antigen + MMANT08 X00882 137 Murine gene fragment for cellular tumour antigen + MMANT09 X00883 74 Murine gene fragment for cellular tumour antigen + MMANT10 X00884 107 Murine gene for cellular tumour antigen p53 (exon + MMANT11 X00885 562 Murine p53 gene 3' region with exon 11 + MMANTP53 M26862 536 Mouse tumor antigen p53 gene, 5' end. + MMLYN M64608 2044 Mouse lyn protein mRNA, complete cds. + MMP53 X00741 1377 Mouse mRNA for transformation associated protein + MMP53A M13872 1285 Mouse p53 mRNA, complete cds, clone pcD53. + MMP53B M13873 1241 Mouse p53 mRNA, complete cds, clone p53-m11. + MMP53C M13874 1322 Mouse p53 mRNA, complete cds, clone p53-m8. + MMP53G1 X01235 554 Mouse genomic DNA for 5' region of cellular tumou + MMP53IN4 X60470 729 M.musculus p53 gene for p53 protein, intron 4 + MMP53P X01236 2132 Mouse pseudogene for cellular tumour antigen p53 + MMP53R X01237 1773 Mouse mRNA for cellular tumour antigen p53 + MMRSB2P5 M64597 196 Mouse B2 repeat in the 3' flank of protein 53 (p5 + 22 different entries found + + Select a task + X 1 Get a sequence + 2 Get annotations + 3 Get entry names from accession numbers + 4 Search titles for keywords + 5 Search text index for keywords + ? Selection (1-5) (1) =4 + Search for keywords + ? Keywords=alpha + Searching for alpha + AAGHA 623 a.anguilla mrna for glycoprotein hormone alpha subunit precu + AAMALI 3338 a.aegypti mali gene encoding alpha 1-4 glucosidase, complete + AAMALIA 1659 a.aegypti maltase-like i (mali) gene encoding alpha-1,4-gluc + AAMALIB 1832 a.aegypti maltase-like i (mali) mrna encoding alpha-1,4-gluc + ACA13GT 371 alouatta caraya alpha-1,3gt gene, 3' flank. + ADHBADA1 102 duck alpha-d-globin gene, exon 1. + ADHBADA2 1145 duck alpha-a-globin gene and 5' flank + ADHBADWP 513 duck (white pekin) alpha ii (minor) globin mrna, complete co + AEACOXABC 5279 a.eutrophus protein x (acox), acetoin:dcpip oxidoreductase-a + AGA13GT 371 ateles geoffroyi alpha-1,3gt gene, 3' flank. + AGAAAGFP 282 c.tetragonoloba alpha-amylase/alpha-galactosidase fusion pro + AGAABL 138 b.subtilis alpha-amylase signal peptide gene e.coli beta-lac + AGAFAMYA 57 synthetic b.stearothermophilus alpha amylase/s.cerevisiae ma + AGAFAMYB 57 synthetic b.stearothermophilus alpha amylase/s.cerevisiae ma + AGAFAMYC 57 synthetic b.stearothermophilus alpha amylase/s.cerevisiae ma + AGAFCOXA 98 synthetic alpha-factor/cox iv fusion gene signal peptide. + AGAGABA 7876 synthetic gossypium hirsutum (cotton) alpha globulin a and b + AGAMYLS 120 synthetic alpha-amylase gene, 5' end. + AGANPS 95 synthetic gene (jcnf-1) encoding alpha-factor pro-region/han +! + Select a task + X 1 Get a sequence + 2 Get annotations + 3 Get entry names from accession numbers + 4 Search titles for keywords + 5 Search text index for keywords + ? Selection (1-5) (1) =3 + ? Accession number=v00636 +Entry name LAMBDA + Select a task + X 1 Get a sequence + 2 Get annotations + 3 Get entry names from accession numbers + 4 Search titles for keywords + 5 Search text index for keywords + ? Selection (1-5) (1) =2 + Default Entry name=LAMBDA + ? Entry name= +ID LAMBDA standard; DNA; PHG; 48502 BP. +XX +AC V00636; J02459; M17233; X00906; +XX +DT 03-JUL-1991 (Rel. 28, Last updated, Version 3) +DT 09-JUN-1982 (Rel. 1, Created) +XX +DE Genome of the bacteriophage lambda (Styloviridae). +XX +KW circular; coat protein; DNA binding protein; genome; +KW origin of replication. +XX +OS Bacteriophage lambda +OC Viridae; ds-DNA nonenveloped viruses; Siphoviridae. +XX +RN [1] +RP 1-48502 +RA Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B.; +RT "Nucleotide sequence of bacteriophage lambda DNA"; +RL J. Mol. Biol. 162:729-773(1982). +XX +! + Select a task + X 1 Get a sequence + 2 Get annotations + 3 Get entry names from accession numbers + 4 Search titles for keywords + 5 Search text index for keywords + ? Selection (1-5) (1) = + Default Entry name=LAMBDA + ? Entry name= +DE Genome of the bacteriophage lambda (Styloviridae). + Sequence length 48502 + Sequence composition + T C A G - + 11988. 11360. 12336. 12818. 0. + 24.7% 23.4% 25.4% 26.4% 0.0% + +.end lit +.left margin1 +@4. TX 1 @Redefine active region +.LEFT MARGIN2 +.para +For its analytic functions +the program always works on a region of the sequence called the active +region. When a new sequence is read into the program the active region is +automatically set to start at the beginning of the sequence and go +up to the +maximum allowed size of active region the version of the program can +handle. The positions are shown on the screen. +On most machines this will be to the end of the sequence. +This option allows the user define a different region. Note that for +convenience in the +listing and translation functions the user is given access to regions +outside the active region. +.left margin1 +@5. TX 1 @List a sequence +.LEFT MARGIN2 +.para +The sequence can be listed with line lengths from +10 to 120 in multiples of 10. Output can be directed to a disk file by +first selecting disk output. The output looks like: +.lit + + 10 20 30 40 50 60 + MQLNSTEISE LIKQRIAQFN VVSEAHNEGT IVSVSDGVIR IHGLADCMQG EMISLPGNRY + + 70 80 90 100 110 120 + AIALNLERDS VGAVVMGPYA DLAEGMKVKC TGRILEVPVG RGLLGRVVNT LGAPIDGKGP + + 130 140 150 160 170 180 + LDHDGFSAVE AIAPGVIERQ SVDQPVQTGY KAVDSMIPIG RGQRELIIGD RQTGKTALAI + + 190 200 210 220 230 240 + DAIINQRDSG IKCIYVAIGQ KASTISNVVR KLEEHGALAN TIVVVATASE SAALQYLARM + + 250 260 270 280 290 300 + PVALMGEYFR DRGEDALIIY DDLSKQAVAY RQISLLLRRP PGREAFPGDV FYLHSRLLER + + 310 320 330 340 350 360 + AARVNAEYVE AFTKGEVKGK TGSLTALPII ETQAGDVSAF VPTNVISITD GQIFLETNLF + + 370 380 390 400 410 420 + NAGIRPAVNP GISVSRVGGA AQTKIMKKLS GGIRTALAQY RELAAFSQFA SDLDDATRKQ + + 430 440 450 460 470 480 + LDHGQKVTEL LKQKQYAPMS VAQQSLVLFA AERGYLADVE LSKIGSFEAA LLAYVDRDHA + + 490 500 510 520 530 540 + PLMQEINQTG GYNDEIEGKL KGILDSFKAT QSW* + +.end lit +.left margin1 +@6. TX 1 @List a text file +.LEFT MARGIN2 +.para +Allows the user to have a text file displayed on the screen. It will appear +one page at a time. +.left margin1 +@7. TX 1 @Direct output to disk +.LEFT MARGIN2 +.para +Used to direct output that would normally appear on the screen to a file. +.para +Select redirection of either text or graphics, and +supply the name of the file that the output should be written to. +.para + The results from the next options selected will not appear on the screen +but will be written to the file. When option 7 is selected again +the file will be +closed and output will again appear on the screen. +.left margin1 +@8. TX 1 @Write active region to disk +.LEFT MARGIN2 +.para +The program has the capability of reading in EMBL, GENBANK, NBRF, GCG +and Staden formats +and of reversing and complementing sequences. This option allows users +to +write the current active sequence to a disk file in Staden format. Hence +it +allows format conversion and crude sequence cutting. +.left margin1 +@9. TX 1 @Edit the sequence +.LEFT MARGIN2 +.para +Used to edit sequences or any other files by giving access to the +computers system editor. For editing sequences the input file should +have already been created using the listing function "list +sequence". +.para +Supply the name of the file to edit. Wait while the system editor is made +ready (can take awhile on a vax). Use the editor. Exit from the editor. If a +sequence has been edited, and you want to process it, affirm that the +sequence should be "made active". The edited sequence will replace the +original sequence. +.para +This editing method is designed to give users access to an editor with +which they are familiar - i.e. the one on their machine, and yet to allow +them to edit a sequence which contains the landmarks they need in +order to know where they are. Users can create files containing simple +listings with numbering, using "list the sequence", and +then edit them with their system editor, using the numbering to know +where they are within the sequence. When the edits are complete they +exit from the editor and the program "analyses" the edited file to extract +only the sequence characters. Define the permitted set of characters to be: +ACDEFGHIKLMNPQRSTVWXYZ-acdefghiklmnpqrstvwxyz. All permitted characters +found in the file will become part of the sequence, all others removed. +.left margin1 +@10. TX 2 @Clear graphics +.LEFT MARGIN2 +.para + Clears the screen of both text and graphics. +.left margin1 +@11. TX 2 @Clear text +.LEFT MARGIN2 +.para + Clears only text from the screen. +.left margin1 +@12. TX 2 @Draw a ruler +.LEFT MARGIN2 +.para +This option +allows the user to draw a ruler or scale along the x axis of the screen to +help identify the coordinates of points of interest. The user can define +the position of the first amino acid to be marked (for example if the +active +region is 1501 to 8000, the user might wish to mark every 1000th amino +acid +starting at either 1501 or 2000 - it depends if the user wishes to treat +the active region as an independent unit with its own numbering starting +at +its left edge, or as part of the whole sequence). The user can also define +the separation of the ticks on the scale and their height. If required the +labelling routine can be used to add numbers to the ticks. +.left margin1 +@13. TX 2 @Use cross hair +.LEFT MARGIN2 +.para +This function puts +a steerable cross on the screen that can be used to find the +coordinates of points in the sequence. The user can move the cross +around using the directional keys; when he hits the space bar the +program will print out the coordinates of the cross in sequence units and +the option will be exited. +.para +If instead, +you hit a , the position will be displayed but the cross will remain on +the screen. +.para +If a letter s is hit the sequence around the cross hair is displayed and +the cross remains on the screen. +.left margin1 +@14. TX 2 @Reset margins +.LEFT MARGIN2 +.para +The positions of each of the plots is defined relative to a users drawing +board which has size 1-10,000 in x and 1-10,000 in y. +Plots for +each option are drawn in a window defined by x0,y0 and xlength,ylength. +Where x0,y0 is the position of the bottom left hand corner of the window, + and xlength is the width of the window and ylength the +height of the window. +.lit + --------------------------------------------------------- 10,000 + 1 1 + 1 -------------------------------------- ^ 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 1 1 ylength 1 + 1 1 1 1 1 + 1 1 1 1 1 + 1 -------------------------------------- v 1 + 1 x0,y0^ 1 + 1 <---------------xlength--------------> 1 + --------------------------------------------------------- 1 + 1 10,000 + +.end lit +All values are in drawing board units (i.e. 1-10,000, 1-10,000). +The default window positions are read from a file "ANALMARG" when the +program is started. Users can have their own file if required. +As all the plots start +at the same position in x and have the same width, x0 and xlength are the +same for all options. Generally users will only want to change the start +level of the window y0 and its height ylength. + This option +allows users to change window positions whilst running the program. +The routine prompts first for the number of the option that the users +wishes +to reposition; then for the y start and height; then for the x start and +length. Note that changes to the x values affect all options. If the user +types only carriage return for any value it will remain unchanged. +The cross-hair can be used to choose suitable heights. +.LEFT MARGIN1 +@15. TX 2 @Label a diagram +.LEFT MARGIN2 +.para +This routine allows users to label any diagrams they have produced. They +are asked to type in a label. When the user types carriage return to finish +typing the label the cross-hair appears on the screen. The user can +position it anywhere on the screen. If the user types R (for right justify) + the label will be +written on the diagram with its right end at the cross-hair position. +If the user types L (for left justify) the label will be written on the +diagram with its left end at the cross hair position. +The +cross-hair will then immediately reappear. The user may put the same +label +on another part of the diagram as before or if he hits the space bar he +will be asked if he wishes to type in another label. +.left margin1 +@16. TX 2 @Display a map +.LEFT MARGIN2 +.para +It is often convenient to plot a map alongside graphed analysis in order +to +indicate features within the sequence. This function allows users to +draw +maps using files arranged in the form of EMBL feature tables. Of course +the +EMBL table are usually only used for nucleic acid sequence annotation +but, +as long as the features are written in the correct format, they can be +employed by this routine. The map is composed of a line representing the +sequence and then further lines denoting the endpoints of each feature +the +user identifies. The user is asked to define height at which the line +representing the sequence should be drawn; then for the feature height; +then for the features to plot. +.left margin1 +@17. TX 1 5 @Short sequence search +.LEFT MARGIN2 +.para +This routine is used to search for exact matches to short sequences. It is +equivalent to the restriction enzyme search in program NIP. It and can +either list matches +or present the results graphically. +.PARA +Select from searching, screen clearing or file listing. Choose a file of +strings and the mode of output required. +.para +The files of short +sequences (strings) and their names +need to be arranged in a particular way. For example +.lit +ACID/D/E// +BASIC/R/K/H// +HYDRO/F/L/I/V/Y// +GLYCO/N-S/N-T// ++/R/K/H// +-/D/E// +.end lit +defines various groups of amino acids. +Each string or set of strings must be +preceded by a name, each string must be preceded and +terminated with a slash (/), and +each set of strings by 2 slashes. These collections of strings and their +names can be read from disk or entered from the keyboard. Two files +containing sequences are currently +available. One contains named groups of amino acids. The other simply +contains the names of all amino acids and gives a convenient way of +producing a plot of the positions of all the different +amino acids in the sequence. +The user can select strings +by name from these collections. Results can be displayed name by name +or all +together. +Strings entered from the keyboard need to be separated by slash +characters(/). +For the name by name search the output looks like: +.lit + MATCHES= 12 + NAME SEQUENCE POSITION FRAGMENT LENGTHS + ACID E 7 7 1 + ACID E 10 3 1 + ACID E 24 14 1 + ACID E 28 4 1 + ACID D 36 8 1 + ACID D 46 10 2 + ACID E 51 5 2 + ACID E 67 16 2 + ACID D 69 2 2 + ACID D 81 12 2 + ACID E 84 3 2 + ACID E 96 12 3 + MATCHES= 10 + NAME SEQUENCE POSITION FRAGMENT LENGTHS + BASIC K 13 13 1 + BASIC R 15 2 1 + BASIC H 26 11 1 + BASIC R 40 14 1 + BASIC H 42 2 2 + BASIC R 59 17 2 + BASIC R 68 9 2 + BASIC K 87 19 2 + BASIC K 89 2 2 + BASIC R 93 4 2 + MATCHES= 1 + NAME SEQUENCE POSITION FRAGMENT LENGTHS + GLYCO NST 4 4 3 + + or when the results are ordered only on position the output looks like: + + NAME SEQUENCE POSITION FRAGMENT LENGTHS + GLYCO NST 4 3 + ACID E 7 3 + ACID E 10 3 + BASIC K 13 3 + BASIC R 15 2 + ACID E 24 9 + BASIC H 26 2 + ACID E 28 2 + ACID D 36 8 + BASIC R 40 4 + BASIC H 42 2 + ACID D 46 4 + ACID E 51 5 + BASIC R 59 8 +.end lit +.LEFT MARGIN2 +Graphical output marks the position of each string by a +short vertical line and gives its name at the left end of the +line. If the top of the screen is reached the program gives the user the +oportunity to take a hard copy and then will clear the screen and restart +plotting results at the original start position. +Note that any character in the string +that is not a recognisable protein symbol will be treated as a +wild card character will match with all +characters in the searched sequence. +.para +.lit +Typical dialogue follows. + +Menus and their numbers are +m0 = This menu +m1 = General +m2 = Screen control +m3 = Statistical analysis of content +m4 = Structure +m5 = Search + ? = Help + ! = Quit +? Menu or option number=17 + Search for short sequences +X 1 Search + 2 List enzyme file + 3 Clear text + 4 Clear graphics +? 0,1,2,3,4 =2 + 1 All acids +X 2 Named groups + 3 Personal file + 4 Keyboard +? 0,1,2,3,4 = + +ACID/D/E// +BASIC/R/K/H// +HYDRO/F/L/I/V/Y// +GLYCO/N-S/N-T// ++/R/K/H// +-/D/E// +DIBASIC/RR/KK/RK/KR// +TURN/N/D/G/P/S// +BLOCK/A/Q/E/I/L/M/F/W/V// +INDIF/R/C/H/K/T/Y// +End of file + + +X 1 Search + 2 List enzyme file + 3 Clear text + 4 Clear graphics +? 0,1,2,3,4 = + + 1 All acids +X 2 Named groups + 3 Personal file + 4 Keyboard +? 0,1,2,3,4 = + +? (y/n) (y) All names n +? Name=acid +? Name=basic +? Name=glyco +? Name= + +? (y/n) (y) Show results name by name +? (y/n) (y) List matches + + searching + matches= 59 +NAME SEQUENCE POSITION FRAGMENT LENGTHS +ACID E 7 7 1 +ACID E 10 3 1 +ACID E 24 14 1 +ACID E 28 4 1 +ACID D 36 8 1 +ACID D 46 10 2 +ACID E 51 5 2 +ACID E 67 16 2 +ACID D 69 2 2 +ACID D 81 12 2 +ACID E 84 3 2 +ACID E 96 12 3 +ACID D 116 20 3 +... etc + matches= 61 +NAME SEQUENCE POSITION FRAGMENT LENGTHS +BASIC K 13 13 1 +BASIC R 15 2 1 +BASIC H 26 11 1 +BASIC R 40 14 1 +BASIC H 42 2 2 +BASIC R 59 17 2 + ...etc + matches= 2 +NAME SEQUENCE POSITION FRAGMENT LENGTHS +GLYCO NST 4 4 3 +GLYCO NQT 487 483 28 + 28 483 + + +X 1 Search + 2 List enzyme file + 3 Clear text + 4 Clear graphics +? 0,1,2,3,4 = + + 1 All acids +X 2 Named groups + 3 Personal file + 4 Keyboard +? 0,1,2,3,4 = + +? (y/n) (y) Selected names + +? Name=basic +? Name=glyco +? Name= + +? (y/n) (y) Show results name by name n +? (y/n) (y) List matches + + searching +NAME SEQUENCE POSITION FRAGMENT LENGTHS +GLYCO NST 4 3 +BASIC K 13 9 +BASIC R 15 2 +BASIC H 26 11 +BASIC R 40 14 +BASIC H 42 2 +BASIC R 59 17 +BASIC R 68 9 +BASIC K 87 19 + ...etc +BASIC R 477 14 +BASIC H 479 2 +GLYCO NQT 487 8 +BASIC K 499 12 +BASIC K 501 2 +BASIC K 508 7 + 7 + +X 1 Search + 2 List enzyme file + 3 Clear text + 4 Clear graphics +? 0,1,2,3,4 = + 1 All acids +X 2 Named groups + 3 Personal file + 4 Keyboard +? 0,1,2,3,4 =4 +Define search strings by typing a string name +followed by the string(s) +? Name=MARY +? String(s)=AL/VI +? Name= +? (y/n) (y) All names +? (y/n) (y) Show results name by name +? (y/n) (y) List matches + + searching + matches= 12 +NAME SEQUENCE POSITION FRAGMENT LENGTHS +MARY VI 38 38 10 +MARY AL 63 25 13 +MARY VI 136 73 16 +MARY AL 177 41 19 +MARY AL 217 40 25 +MARY AL 233 16 37 +MARY AL 243 10 40 +MARY AL 256 13 41 +MARY AL 326 70 45 +MARY VI 345 19 51 +MARY AL 396 51 70 +MARY AL 470 74 73 + + +.END LIT + +.left margin1 +@18. TX 1 5 @Compare a sequence +.LEFT MARGIN2 +.para +This routine slides a short sequence along the current sequence and finds +all positions at which a given percentage of the amino acids match. +Output is in both graphical and listed forms. +.para +If users call for dialogue when the routine is selected they will be given +the choice of keyboard or file input. Define the string, and the percentage +match. Matches will be plotted out and then the user can select to have +them listed. Then the routine cycles around. +.para + The routine slides the search string +along the sequence and marks the positions at which a minimum +percentage score is reached. The graphical output draws a vertical line at +the match position; the height of the line represents the percentage +score, +so that if the line reaches the top of the box the score is 100%. +.para +Typical dialogue follows. +.lit + +? Menu or option number=18 + Find percentage matches +? (y/n) (y) Keep picture + +? String=aaa +? Percent match (1.00-100.00) (70.00) = + + missing graphics + +Total scoring positions above 70.000 percent = 19 +Scores 2 2 2 2 2 2 2 2 2 2 +Positions 61 131 177 217 226 231 232 267 300 301 + +? Number to list (0-19) (0) =3 + + 61 + AIA + * * + aaa + 1 + + 131 + AIA + * * + aaa + 1 + + 177 + ALA + * * + aaa + 1 +? (y/n) (y) Keep picture n + +Default String=aaa +? String=! + +.end lit + +.left margin1 +@19. TX 1 5 @Compare a sequence using a score matrix +.LEFT MARGIN2 +.para +This routine slides a short sequence along the current sequence and finds +all positions at which a given level of similarity (a cutoff score) is +reached. The score is defined by use of a score matrix (MDM78). Output is +in both graphical and listed forms. +.para +If users call for dialogue when the routine is selected they will be given +the choice of keyboard or file input. Define the string and the cutoff +score. Matches will be plotted out and then the user can select to have +them listed. Then the routine cycles around. +.para + The routine slides the search string +along the sequence and marks the positions at which a the cutoff score +is achieved. The graphical output draws a vertical line at +the match position; the height of the line represents the score, +so that if the line reaches the top of the box the score is the maximum +possible. +.para +Typical dialogue follows. +.lit + +Menus and their numbers are +m0 = This menu +m1 = General +m2 = Screen control +m3 = Statistical analysis of content +m4 = Structure +m5 = Search + ? = Help + ! = Quit +? Menu or option number=19 + Find matches using a score matrix +? (y/n) (y) Keep picture + +? String=aaa +Minimum score= 12 Maximum score= 36 +? Score (12-36) (36) = + + missing graphics + +For score 24 the number of matches= 507 +scores 35 35 35 34 34 34 34 34 34 34 +positions 226 231 379 112 133 202 227 267 378 +380 + +? Number to list (0-507) (0) =3 + + 226 + ATA + * * + aaa + 1 + + 231 + SAA + ** + aaa + 1 + + 379 + GAA + ** + aaa + 1 +? (y/n) (y) Keep picture n + +Default String=aaa +? String=! +.end lit +.left margin1 +@20. TX 5 @Search for a motif using a weight matrix +.LEFT MARGIN2 +.para +This function performs searches for short sequence +motifs using an appropriate weight matrix. In addition it can be used to +create or modify weight matrices. In order to perform a search the only +input +required is the name of the file containing the weight matrix. +The results can be presented graphically or listed. The graphical +presentation will draw line at the position of any matches found; the +height of the line is proportional to the score. +.para +For a search, select "use weight matrix", supply the name of the file +containing the weight matrix, and choose between having results plotted +or listed. If dialogue is requested when the function is selected users can +alter the cutoff score employed. +.para +To create a weight matrix several steps are involved. A file containing an +alignment of known motifs is required. (This file must be created before +the current option is selected. The format is a follows: each sequence is +written on a separate line with at least one space at the beginning; each +sequence is terminated by a space character, and can be followed by a +name. The sequences must be aligned.) Supply the name of the file of +aligned sequences. The program reads and displays the sequences. Choose +between "summing logs of weights" or summing weights (i.e. whether to +multiply or add weights). If logs are used all scores will be negative. +Choose if all positions in the set of aligned sequences should be used or +if a mask should be applied. If so selected, define a mask as a string of +symbols, in which symbol - means ignore and any other symbol means +use. E.g. xx-x--abc means use all positions except 3,5 and 6. +.para +The program will calculate weights as the frequencies of each amino +acid at each unmasked position in the set of aligned sequences. These +weights are then applied to the set of aligned sequences to give a range +of "observed" scores. The mean and standard deviation of these scores is +displayed. The user is asked to supply several values to be used when the +weight matrix is applied to other sequences: a cutoff score (by default, +the mean minus 3 standard deviations), a top score for scaling graphical +results (by default, the mean plus 3 standard deviations), and a position +to identify (this means that if a particular amino acid within the motif +is used as a "landmark", such as the G of the helix-turn-helix motif, then +its position will be marked in plots). All these values are stored along +with the weight matrix. Finally supply the name of a file to contain the +weight matrix. +.para +Weight matrices can be "rescaled" using a set of aligned sequences in +much the same ways as a matrix is created. The purpose is to redefine +the cutoff scores, and rescaling does not alter any other values in the +weight matrix file. +.para +The methods have changed considerably but were first outlined in +Staden, R. Nucl. Acid Res. 12 505-519 1984, and +Staden, R. Genetic +engineering: principles and methods vol 7, Edited by J.K. Setlow and A. +Hollaender, Plenum publishing corp., 1985. +.para + The methods have always had to deal with the problem of zeroes in the +matrices. The current versions +employ "Laplaces Law of Succession" in which 1 is +added to each term. +.para +It is now possible to apply a mask to a set of aligned sequences in +order to give weight to selected positions only. +Sequences have superimposed functions: some parts may be of general +structural +importance and give rise to an overall framework, and other parts give +specificity and hence are not common; we may want to use a set of +aligned +sequences to define a motif, but want to use only the framework +positions. + Alternatively we may want to pick out +only those parts of a set of aligned sequences that give a particular +property, and to ignore other similarities that are due to some other +property +and which could obscure the pattern +we are interested in. The ability to define a mask allows certain +positions +to be used in the motif and others to be ignored, and yet still permits the +use of a set of aligned sequences to calculate weights. +.para +Typical dialogue is shown below. +.lit +? Menu or option number=20 +X 1 Use weight matrix + 2 Make weight matrix + 3 Rescale weight matrix +? 0,1,2,3 =2 +? Name of aligned sequences file=[rs.motifs]hth.seq + 1 QESVADKMGMGQSGVGALFN LAMBDA.REP + 2 QTKTAKDLGVYQSAINKAIH LAMBDA.CRO + 3 QAALGKMVGVSNVAISQWQR P22.REP + 4 QRAVAKALGISDAAVSQWKE P22.CRO + 5 QAELAQKVGTTQQSIEQLEN 434.REP + 6 QTELATKAGVKQQSIQLIEA 434.CRO + 7 RQEIGQIVGCSRETVGRILK CAP + 8 RGDIGNYLGLTVETISRLLG Fnr + 9 LYDVAEYAGVSYQTVSRVVN LAC.R + 10 IKDVARLAGVSVATVSRVIN GAL.R + 11 TEKTAEAVGVDKSQISRWKR LAMBDA.CII + 12 QRKVADALGINESQISRWKG P22.CI + 13 KEEVAKKCGITPLQVRVWCN MAT.ALPHA + 14 TRKLAQKLGVEQPTLYWHVK TETR.TN10 + 15 TRRLAERLGVQQPALYWHFK TETR.pSC1 + 16 QRELKNELGAGIATITRGSN TRP.REP + 17 RQQLAIIFGIGVSTLYRYFP H-INVERSN + 18 ATEIAHQLSIARSTVYKILE TN3.RESOL + 19 ASHISKTMNIARSTVYKVIN GD.RESOLV + 20 IASVAQHVCLSPSRLSHLFR ARA.C + 21 RAEIAQRLGFRSPNAAEEHL LEX.R +Length of motif 20 +? (y/n) (y) Sum logs of weights +? (y/n) (y) Use all motif positions n +x means use, - means ignore +e.g. xx-x---x-x means use positions 1,2,4,8,10 +? Mask=--xxxxxxxxxxxx------ + Applying weights to input sequences + 1 -57.143 QESVADKMGMGQSGVGALFN + 2 -55.087 QTKTAKDLGVYQSAINKAIH + 3 -58.079 QAALGKMVGVSNVAISQWQR + 4 -54.986 QRAVAKALGISDAAVSQWKE + 5 -55.181 QAELAQKVGTTQQSIEQLEN + 6 -55.874 QTELATKAGVKQQSIQLIEA + 7 -56.692 RQEIGQIVGCSRETVGRILK + 8 -57.722 RGDIGNYLGLTVETISRLLG + 9 -55.363 LYDVAEYAGVSYQTVSRVVN + 10 -55.769 IKDVARLAGVSVATVSRVIN + 11 -56.786 TEKTAEAVGVDKSQISRWKR + 12 -55.833 QRKVADALGINESQISRWKG + 13 -56.279 KEEVAKKCGITPLQVRVWCN + 14 -53.125 TRKLAQKLGVEQPTLYWHVK + 15 -55.833 TRRLAERLGVQQPALYWHFK + 16 -58.651 QRELKNELGAGIATITRGSN + 17 -56.749 RQQLAIIFGIGVSTLYRYFP + 18 -56.986 ATEIAHQLSIARSTVYKILE + 19 -60.618 ASHISKTMNIARSTVYKVIN + 20 -58.988 IASVAQHVCLSPSRLSHLFR + 21 -58.002 RAEIAQRLGFRSPNAAEEHL +Top score -53.125 Bottom score -60.618 +Mean -56.655 Standard deviation 1.617 +Mean minus 3.sd -61.505 Mean plus 3.sd -51.804 +? Cutoff score (-999.00-9999.00) (-61.51) = +? Top score for scaling plots (-61.51-999.00) (-51.80) = +? Position to identify (0-20) (1) =9 +? Title=hth +? Name for new weight matrix file=1.wts + +Menus and their numbers are +m0 = This menu +m1 = General +m2 = Screen control +m3 = Statistical analysis of content +m4 = Structure +m5 = Search + ? = Help + ! = Quit +? Menu or option number=20 +X 1 Use weight matrix + 2 Make weight matrix + 3 Rescale weight matrix +? 0,1,2,3 = + +? Motif weight matrix file=1.wts + hth +? (y/n) (y) Use frequencies as weights +? (y/n) (y) Plot results n + 5 -61.46 STEISELIKQRIAQFNVVSE + 13 -58.93 KQRIAQFNVVSEAHNEGTIV + 21 -60.42 VVSEAHNEGTIVSVSDGVIR + 57 -59.39 GNRYAIALNLERDSVGAVVM + 59 -61.47 RYAIALNLERDSVGAVVMGP + 79 -59.90 YADLAEGMKVKCTGRILEVP + 88 -61.41 VKCTGRILEVPVGRGLLGRV + 104 -60.38 LGRVVNTLGAPIDGKGPLDH + 127 -60.13 SAVEAIAPGVIERQSVDQPV + 129 -59.91 VEAIAPGVIERQSVDQPVQT + 133 -60.79 APGVIERQSVDQPVQTGYKA + 139 -61.12 RQSVDQPVQTGYKAVDSMIP + 175 -58.90 KTALAIDAIINQRDSGIKCI + 191 -60.95 IKCIYVAIGQKASTISNVVR + 195 -60.94 YVAIGQKASTISNVVRKLEE + 215 -60.66 HGALANTIVVVATASESAAL + 254 -60.56 EDALIIYDDLSKQAVAYRQI + 260 -60.08 YDDLSKQAVAYRQISLLLRR + 297 -61.00 LLERAARVNAEYVEAFTKGE + 314 -61.29 KGEVKGKTGSLTALPIIETQ + 330 -60.49 IETQAGDVSAFVPTNVISIT + 363 -57.63 GIRPAVNPGISVSRVGGAAQ + 365 -61.48 RPAVNPGISVSRVGGAAQTK + 371 -61.02 GISVSRVGGAAQTKIMKKLS + 382 -57.90 QTKIMKKLSGGIRTALAQYR + 394 -60.07 RTALAQYRELAAFSQFASDL + 424 -59.95 GQKVTELLKQKQYAPMSVAQ + 430 -58.89 LLKQKQYAPMSVAQQSLVLF + 432 -61.14 KQKQYAPMSVAQQSLVLFAA + 438 -58.58 PMSVAQQSLVLFAAERGYLA + 458 -61.06 DVELSKIGSFEAALLAYVDR + 466 -61.00 SFEAALLAYVDRDHAPLMQE + 483 -60.48 MQEINQTGGYNDEIEGKLKG + 494 -60.61 DEIEGKLKGILDSFKATQSW + +Menus and their numbers are +m0 = This menu +m1 = General +m2 = Screen control +m3 = Statistical analysis of content +m4 = Structure +m5 = Search + ? = Help + ! = Quit +? Menu or option number=d20 +X 1 Use weight matrix + 2 Make weight matrix + 3 Rescale weight matrix +? 0,1,2,3 = + +? Motif weight matrix file=1.wts + hth +? (y/n) (y) Use frequencies as weights +? Cutoff score (-9999.00-9999.00) (-61.51) =-56. +? (y/n) (y) Plot results n + + +.end lit +.left margin1 +@21. TX 3 @Calculate amino acid composition +.LEFT MARGIN2 +.para +This function calculates the amino acid composition and molecular +weight +for the active region. +.lit +? Menu or option number=21 + Sequence composition + +A C S T P A G N D E Q B Z H +N 3. 32. 23. 18. 57. 47. 16. 28. 31. 28. 0. 0. 7. +% 0.6 6.2 4.5 3.5 11.1 9.1 3.1 5.4 6.0 5.4 0.0 0.0 1.4 +W 309. 2786. 2325. 1748. 4051. 2682. 1826. 3222. 4003. 3588. 0. 0. +960. + +A R K M I L V F Y W - X ? +N 30. 24. 11. 40. 47. 41. 14. 15. 1. 0. 0. 0. 1. +% 5.8 4.7 2.1 7.8 9.1 8.0 2.7 2.9 0.2 0.0 0.0 0.0 0.2 +W 4686. 3076. 1443. 4527. 5319. 4065. 2060. 2448. 186. 0. 0. 0. +0. +Total molecular weight= 55328. + +.end lit +.left margin1 +@22. TX 3 4 @Plot hydrophobicity +.LEFT MARGIN2 +.para +This routine plots the hydrophobicity of each section of the sequence +using +the hydrophobicity +values of Kyte and Doolittle (J. Mol. Biol. 157, 105-132 (1982)). +A window of size span is slid along the sequence and a sum calculated +for +each position. +.para +If dialogue is requested select a span length and a plot interval. +.para +The diagrams are on the same scale as Fig. 6 of the Kyte and Doolittle +paper and values of + and - 50 could be assigned to the top and bottom of +the diagram with corresponding values in between (-40,-20,0,20,40 are +shown +in the paper). +.lit +? Menu or option number=d22 + Plot hydrophobicity +? odd span length (1-101) (11) = +? plot interval (1-101) (3) = + + missing graphics +.end lit +.LEFT MARGIN1 +@23. TX 3 4 @Plot charge +.LEFT MARGIN2 +.para +This routine plots the charge of each section of the sequence. +A window of size span is slid along the sequence and a sum calculated +for +each position. Amino acids are assigned charges of 1, -1 or 0. +.para +If dialogue is requested select a span length and a plot interval. +.para +Typical dialogue follows. +.lit + +? Menu or option number=d23 + Plot charge +? odd span length (1-101) (11) = +? plot interval (1-101) (3) = + + missing graphics + +.end lit +.LEFT MARGIN1 +@24. TX 4 @Plot robson prediction +.LEFT MARGIN2 +.para +This routine uses the method of Garnier J, Osguthorpe D J, and Robson B. +(1978) J. Mol. Biol. 120, 97-120 to predict secondary structures. The +method divides protein secondary structures into 4 classes: helix, +extended +(usually referred to as sheet), turn and coil. The routine calculates the +likelihood that each segment of the sequence lies in each of these +classes. Results are presented graphically or listed. +.para +If dialogue is requested choose between plotted or listed output. +.para + Each residue +has a +certain probability of being found in each of the 4 classes. This +probability +depends both on its own amino acid type and also the 8 +amino acids found to either side along the protein chain. Four tables of +weights, each 20 by 17 elements are used to calculate the likelihood that +each residue along the chain falls into one of the four classes of +structure. The most likely structure at each point +is the one with the highest score. +The four values are plotted in strips labelled H, E, T and C. +Below, a strip labelled D for decision is divided into four levels, each +corresponding to one of the four structure types. Their top to bottom +order +is the same as that for the strips above, i.e C, T, E, and H. For each +residue the program measures which of the four likelhoods is highest. It +places a single dot at the + mid-point of the corresponding strip, and +also at the +appropriate level in the strip labelled D. +.PARA +It should be noted that the method, when tested by Kabsch W and Sander +C, +(1983) Febs. Lett. 155 (179-182), although one of the better ones, was +correct for only about 56% of residues. +.para +Typical dialogue follows. +.lit +? Menu or option number=d24 + Plot Robson secondary structure predictions +? (y/n) (y) Plot results n + + 9 S 217 -7 -39 15 + 10 E 226 5 -27 -39 + 11 L 233 -7 -26 -15 + 12 I 229 -23 9 4 + 13 K 214 -8 10 -8 + 14 Q 178 42 19 5 + 15 R 131 54 16 3 + 16 I 86 42 -31 -23 + 17 A 55 52 -30 -15 + 18 Q 15 67 4 25 + 19 F -34 86 47 74 + 20 N -41 74 17 106 + 21 V -16 118 -5 100 + 22 V 64 88 5 115 + 23 S 96 38 26 155 + 24 E 133 -25 13 96 + 25 A 118 -98 25 100 + 26 H 110 -150 37 86 + 27 N 57 -201 37 66 + 28 E 51 -140 11 -4 + 29 G 2 -77 37 9 + 30 T 2 28 28 7 + 31 I -11 117 -21 22 + 32 V -23 178 -55 5 + 33 S -54 193 -14 35 + 34 V -46 123 5 30 + 35 S -54 53 51 80 + 36 D -60 1 86 55 + 37 G -66 8 57 49 + 38 V -1 128 -30 -5 + 39 I 11 212 -56 -33 + 40 R 16 204 -44 -57 + ...etc + +.end lit +.LEFT MARGIN1 +@26. TX 4 @Draw a helix wheel +.LEFT MARGIN2 +.para +A helical representation of segments of the sequence is shown. The +display +includes a schematic of the helix showing the links between residues, +with +each vertex numbered according to position; the sequence element at +each +vertex; a symbol denoting a classification as hydrophobic(.), positively +charged(+), negatively charged(-), or otherwise( ). The +residue number of the first sequence element in +the current window is displayed at the top-left-hand +corner of the diagram. Also at the top-left corner the sequence in the +current window is listed. Below this is the total hydrophobicity and +hydrophobic moment for the window calculated according to Eisenberg et +al +J. Mol. Biol. 179, 125-142 (1984). +.para +If dialogue is requested the user is asked for the angle to define the turn +between residues as seen +looking along the helix, and a window length. The window length can be up +to 60, with default 18, and the angle has a default of 100 degrees. Note +that 18 x 100 is 5 turns. When the option is selected the first segment in +the current active region is displayed then the bell rings. If the user +types only return, the display will click on by one residue; if another +number is typed, say N, then the display will click forwards (or +backwards +if N is negative) by N residues. If the wheel runs off either end of the +sequence the option will be exited. +.para +Typical dialogue follows. +.lit +? Menu or option number=d26 +? Angle (1-130) (100) = +? Window (1-60) (18) = + + missing graphics + +.end lit +.left margin1 +@25. TX 3 4 @Plot hydrophobic moment +.LEFT MARGIN2 +.para +This routine plots hydrophobic moment and hydrophobicity according to +Eisenberg et al +J. Mol. Biol. 179, 125-142 (1984). The mean hydrophobicity per residue in +the window is plotted on a scale -1.0 to 1.5, and the mean hydrophobic +moment per residue on a scale 0.0 to 1.5. +The hydrophobicity is shown in the top frame with the +hydrophobic moment below. +The plot is arranged so that the +value shown at position x represents the mean value for residues x- +window+1 +to x, where window is the window length. +.para +If dialogue is requested the user can select a window +length, and the angle used for the hydrophobic moment +calculation. +.para +Note that according to Eisenberg et al, in transmembrane proteins an +"initiator" is required. This is either a very hydrophobic single helix +with >=0.68, or a moderately hydrophobic pair of helices whose +sum +to >= 1.1. Other helices are then accepted as transmembrane if their +>= +0.42 +.para +The following rules are claimed: if < 0.51 and points lie below the +line = -0.392 + 0.603x they are "globular", if they lie above this +line they are "surface". If > 0.51 and they lie above the line = +0.6 - 0.342x they are "monomeric", if above "multimeric". +.para +Typical dialogue follows. +.lit + +? Menu or option number=d25 +? Angle (1-130) (100) = +? Window (1-60) (18) = +? Plot interval (1-101) (3) = + + missing graphics + + +.end lit +.left margin1 +@27. TX 1 @Back translate to dna +.LEFT MARGIN2 +.para +This routine back translates protein sequences into DNA using the +standard +genetic code. The level of redundancy can be plotted and the +backtranslation saved to a file. +.para +The translation can use either the IUB symbols shown below, or a set of +codon +preferences. If a set of codon preferences are used they must conform to +the format of codon tables produced by the nucleotide analysis +program, and the back +translation +will contain the favoured codons. If there is no favoured codon +the IUB symbols will be employed. The window length for +plotting the redundancy is in codons. +.para +The program will plot the redundancy along the sequence and hence can +be +used to find the best sequences to use as primers. Note that the program +plots the inverse, and so the higher the +plot the LESS redundant the sequence. For primers look for peaks rather +than +troughs. +.para +The DNA sequence can be saved to a file and analysed using the nucleotide +analysis program. +Depending on the application it is often useful to produce a back +translation using both a table of codon preferences and one using the IUB +symbols. This is because the restriction enzyme search program can +distinguish between definite and possible cuts in the sequence. +These matches are what the program terms "definite matches" and are +ones in +which the specification of the recognition sequence corresponds +exactly to that of the back translation. The program will also find what +it +terms "possible matches" which are ones that depend on the particular +codons +chosen for each amino acid. +These are sites at which recognition +sequences could be engineered to produce a cut in the DNA +without changing the amino +acid, but which are not +necessarily found in the original sequence. +.LIT + + + NC-IUB SYMBOLS + + A,C,G,T + R (A,R) 'puRine' + Y (T,C) 'pYrimidine' + W (A,T) 'Weak' + S (C,G) 'Strong' + M (A,C) 'aMino' + K (G,T) 'Keto' + H (A,T,C) 'not G' + B (G,C,T) 'not A' + V (G,A,C) 'not T' + D (G,A,T) 'not C' + N (G,A,C,T) 'aNy' + + Typical dialogue follows. + +? Menu or option number=d27 + Back translate +? (y/n) (y) No codon preference +? (y/n) (y) Plot redundancy n +? (y/n) (y) Save DNA to disk +? File name for DNA sequence=tt: +ATGCARYTNAAYWSNACNGARATHWSNGARYTNATHAARCARMGNATHGCNCARTTYAAY +GTNGTNWSNGARGCNCAYAAYGARGGNACNATHGTNWSNGTNWSNGAYGGNGTNATHMGN +ATHCAYGGNYTNGCNGAYTGYATGCARGGNGARATGATHWSNYTNCCNGGNAAYMGNTAY +GCNATHGCNYTNAAYYTNGARMGNGAYWSNGTNGGNGCNGTNGTNATGGGNCCNTAYGCN +GAYYTNGCNGARGGNATGAARGTNAARTGYACNGGNMGNATHYTNGARGTNCCNGTNGGN +MGNGGNYTNYTNGGNMGNGTNGTNAAYACNYTNGGNGCNCCNATHGAYGGNAARGGNCCN +YTNGAYCAYGAYGGNTTYWSNGCNGTNGARGCNATHGCNCCNGGNGTNATHGARMGNCAR +WSNGTNGAYCARCCNGTNCARACNGGNTAYAARGCNGTNGAYWSNATGATHCCNATHGGN +MGNGGNCARMGNGARYTNATHATHGGNGAYMGNCARACNGGNAARACNGCNYTNGCNATH +GAYGCNATHATHAAYCARMGNGAYWSNGGNATHAARTGYATHTAYGTNGCNATHGGNCAR +AARGCNWSNACNATHWSNAAYGTNGTNMGNAARYTNGARGARCAYGGNGCNYTNGCNAAY +ACNATHGTNGTNGTNGCNACNGCNWSNGARWSNGCNGCNYTNCARTAYYTNGCNMGNATG +CCNGTNGCNYTNATGGGNGARTAYTTYMGNGAYMGNGGNGARGAYGCNYTNATHATHTAY +GAYGAYYTNWSNAARCARGCNGTNGCNTAYMGNCARATHWSNYTNYTNYTNMGNMGNCCN +CCNGGNMGNGARGCNTTYCCNGGNGAYGTNTTYTAYYTNCAYWSNMGNYTNYTNGARMGN +GCNGCNMGNGTNAAYGCNGARTAYGTNGARGCNTTYACNAARGGNGARGTNAARGGNAAR +ACNGGNWSNYTNACNGCNYTNCCNATHATHGARACNCARGCNGGNGAYGTNWSNGCNTTY +GTNCCNACNAAYGTNATHWSNATHACNGAYGGNCARATHTTYYTNGARACNAAYYTNTTY +AAYGCNGGNATHMGNCCNGCNGTNAAYCCNGGNATHWSNGTNWSNMGNGTNGGNGGNGCN +GCNCARACNAARATHATGAARAARYTNWSNGGNGGNATHMGNACNGCNYTNGCNCARTAY +MGNGARYTNGCNGCNTTYWSNCARTTYGCNWSNGAYYTNGAYGAYGCNACNMGNAARCAR +YTNGAYCAYGGNCARAARGTNACNGARYTNYTNAARCARAARCARTAYGCNCCNATGWSN +GTNGCNCARCARWSNYTNGTNYTNTTYGCNGCNGARMGNGGNTAYYTNGCNGAYGTNGAR +YTNWSNAARATHGGNWSNTTYGARGCNGCNYTNYTNGCNTAYGTNGAYMGNGAYCAYGCN +CCNYTNATGCARGARATHAAYCARACNGGNGGNTAYAAYGAYGARATHGARGGNAARYTN +AARGGNATHYTNGAYWSNTTYAARGCNACNCARWSNTGG--- + + +.end lit + +.LEFT MARGIN1 +@28. TX 5 @Search for patterns of motifs +.left margin2 +.para +This option searches for patterns of motifs. Patterns can be defined +interactively or read from files. Results can be displayed in several ways +in both graphical and textual form. Used to create pattern files for +searching libraries. The option is extremely flexible and consequently the +following documentation is quite lengthy. However the routine is capable +of searching for almost any known pattern. In addition the flexibility +does not necessitate difficulty of use, and the userinterface has been +simplified considerably since the methods were first published. +.para +Users should refer to the "typical dialogue" shown below for the most +helpful information on using the program. +.para +There are currently +four ways to display the matching patterns: 1=each individual +motif and its position is listed; 2=all the sequence between, and +including the two +outermost motifs is listed; 3=graphical, with a vertical line marking the +position +of the leftmost motif; 4 = EMBL feature table format, where the KEYNAM +field is the motif name, the FROM and TO fields denote the ends of the +match, and the DESCRIPTION field is "Program". +.para +When it is defined for the first time a pattern must be entered +interactively at the keyboard, but the pattern description +can be saved to a file. +This file can be used for all subsequent searches. +.para +When defining a pattern interactively +select a motif class and the program will request the required inputs. +.para +The program gives each motif an identifying name and number. +For motifs other than the first, a range of allowed positions must be +defined (Note that sets of motifs included using the OR operator will all +be given the same range, and so the program will only request range +values +for the first motif in any such set). +To specify the allowed range for a motif the user must supply the +following: the +identifying number of the motif, relative to which the current motifs +positions are to be defined (termed the "reference motif"); a "relative start +position" and a range. The relative start position can be negative or positive. +A negative start position means that although the reference motif +is searched for first, the current motif can be found to its left. +A zero relative start position means their left ends are superimposed. The +default start position is to butt-joint the motif to righthand end of the +"reference motif". The range is "the number of extra positions" that the +motif can take. +.para +The program will display the probability of finding each motif. These +values are presented in the following form: .1234E-5 means 0.1234 times +10 +to the power -5. +.para +After the pattern has been defined, the program will type a description +of +it on the screen. It will then allow the user to give an overall cutoff +score and overall probability cutoff. +.para +Typical dialogue for all the different motif classes is displayed below. +.lit + +? Menu or option number=28 + Pattern searcher +? (y/n) (y) Read pattern from keyboard +X 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Direct repeat + 6 Membership of set + 7 Pattern complete +? 0,1,2,3,4,5,6,7 = +? Motif name=aa +? String=aa +Probability of score 2.0000 = 0.123E-01 +X 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Direct repeat + 6 Membership of set + 7 Pattern complete +? 0,1,2,3,4,5,6,7 =2 +? Motif name=pmatch +X 1 And + 2 Or + 3 Not +? 0,1,2,3 = +? Number of reference motif (1-1) (1) = +? Relative start position (-1000-1000) (3) = +? Number of extra positions (0-1000) (0) = +? String=qqq +? Minimum matches (1.00-3.00) (3.00) =2 +Probability of score 2.0000 = 0.858E-02 + 1 Exact match +X 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Direct repeat + 6 Membership of set + 7 Pattern complete +? 0,1,2,3,4,5,6,7 =3 +? Motif name=sm +X 1 And + 2 Or + 3 Not +? 0,1,2,3 = +? Number of reference motif (1-2) (2) = +? Relative start position (-1000-1000) (4) = +? Number of extra positions (0-1000) (0) = +? String=wqa +? Minimum score (11.00-53.00) (53.00) =36 +Probability of score 36.0000 = 0.531E-02 + 1 Exact match + 2 Percentage match +X 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Direct repeat + 6 Membership of set + 7 Pattern complete +? 0,1,2,3,4,5,6,7 =4 +? Motif name=hth +X 1 And + 2 Or + 3 Not +? 0,1,2,3 = +? Number of reference motif (1-3) (3) = +? Relative start position (-1000-1000) (4) = +? Number of extra positions (0-1000) (0) = +? Weight matrix file name=hth + HELIX TURN HELIX PABO SAUER WEIGHTS 17-11-87 +Probability of score -51.5860 = 0.230E-04 + 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix +X 4 Cut-off score and weight matrix + 5 Direct repeat + 6 Membership of set + 7 Pattern complete +? 0,1,2,3,4,5,6,7 =5 +? Motif name=repeat +X 1 And + 2 Or + 3 Not +? 0,1,2,3 = +? Number of reference motif (1-4) (4) = +? Relative start position (-1000-1000) (21) = +? Number of extra positions (0-1000) (0) =3 +? Repeat length (1-60) (6) =3 +? Minimum gap (0-60) (0) = +? Maximum gap (0-60) (0) =2 +? Minimum score (11.00-60.00) (36.00) = +Probability of score 36.0000 = 0.445E-01 + 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix +X 5 Direct repeat + 6 Membership of set + 7 Pattern complete +? 0,1,2,3,4,5,6,7 =6 +? Motif name=mset +X 1 And + 2 Or + 3 Not +? 0,1,2,3 = +? Number of reference motif (1-5) (5) = +? Relative start position (-1000-1000) (1) = +? Number of extra positions (0-1000) (0) = +X 1 Keyboard input + 2 File input +? 0,1,2 = +Separate sets with commas +? String=AVL,AST,,WYRF +? Minimum matches (1.00-4.00) (4.00) =3 +Probability of score 3.0000 = 0.718E-02 + 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Direct repeat +X 6 Membership of set + 7 Pattern complete +? 0,1,2,3,4,5,6,7 =7 +? (y/n) (y) Save pattern in a file +? Pattern definition file=EXAM.PAT +Motif 6 needs a file name to store set as a weight matrix +? Weight matrix file name=DEMO.WTS +Weight matrix needs a title +? Title=Demonstration class 6 weight matrix + +Pattern description + +Motif 1 named aa is of class 1 +Which is an exact match to the string +aa +Motif 2 named pmatch is of class 2 +which is a match of score 2. to the string +qqq +and the N-terminal residue can take positions 3 to 3 +relative to the N-terminal end of motif 1 +It is anded with the previous motif. +Motif 3 named sm is of class 3 +which is a match of score 36. to the string +wqa +and the N-terminal residue can take positions 4 to 4 +relative to the N-terminal end of motif 2 +It is anded with the previous motif. +Motif 4 named hth is of class 4 +Which is a match to a weight matrix with score -51.586 +and the N-terminal residue can take positions 4 to 4 +relative to the N-terminal end of motif 3 +It is anded with the previous motif. +Motif 5 named repeat is of class 5 +Which is a repeat with repeat length 3 and score 36. +The loop-out can have sizes 0 to 2 +and the N-terminal residue can take positions 21 to 24 +relative to the N-terminal end of motif 4 +It is anded with the previous motif. +Motif 6 named mset is of class 6 +Which is membership of a set with score 3.000 +It is anded with the previous motif. +Probability of finding pattern = 0.4109E-14 +Expected number of matches = 0.2539E-10 +? Maximum pattern probability (0.00-1.00) (1.00) = +? Minimum pattern score (-9999.00-9999.00) (-9999.00) = + Select display mode +X 1 Motif by motif + 2 Inclusive + 3 Graphical + 4 EMBL feature table +? 0,1,2,3,4 = + Searching + +Total matches found 0 +Menus and their numbers are +m0 = This menu +m1 = General +m2 = Screen control +m3 = Statistical analysis of content +m4 = Structure +m5 = Search + ? = Help + ! = Quit +? Menu or option number=6 +Page through text files +? Name of file to read=exam.pat + A1 aa Class + aa + @ End of string + A2 pmatch Class + 1 Relative motif + 3 Relative start position + 0 Number of extra positions + qqq + @ End of string + 2.00000 Cutoff + A3 sm Class + 2 Relative motif + 4 Relative start position + 0 Number of extra positions + wqa + @ End of string + 36.00000 Cutoff + A4 hth Class + 3 Relative motif + 4 Relative start position + 0 Number of extra positions +hth File name + A5 repeat Class + 4 Relative motif + 21 Relative start position + 3 Number of extra positions + 3 Length + 0 Minimum loop + 2 Maximum loop + 36.00000 Cutoff + A6 mset Class + 5 Relative motif + 1 Relative start position + 0 Number of extra positions +DEMO.WTS File name +End of file +Menus and their numbers are +m0 = This menu +m1 = General +m2 = Screen control +m3 = Statistical analysis of content +m4 = Structure +m5 = Search + ? = Help + ! = Quit +? Menu or option number=6 +Page through text files +? Name of file to read=demo.wts + Demonstration class 6 weight matrix + 4 0 3.000 4.000 + P 1 2 3 4 + N 0 0 0 0 + C 0 0 0 0 + S 0 1 0 0 + T 0 1 0 0 + P 0 0 0 0 + A 1 1 0 0 + G 0 0 0 0 + N 0 0 0 0 + D 0 0 0 0 + E 0 0 0 0 + Q 0 0 0 0 + B 0 0 0 0 + Z 0 0 0 0 + H 0 0 0 0 + R 0 0 0 1 + K 0 0 0 0 + M 0 0 0 0 + I 0 0 0 0 + L 1 0 0 0 + V 1 0 0 0 + F 0 0 0 1 + Y 0 0 0 1 + W 0 0 0 1 +End of file +Menus and their numbers are +m0 = This menu +m1 = General +m2 = Screen control +m3 = Statistical analysis of content +m4 = Structure +m5 = Search + ? = Help + ! = Quit +? Menu or option number=28 + Pattern searcher +? (y/n) (y) Read pattern from keyboard +X 1 Exact match + 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Direct repeat + 6 Membership of set + 7 Pattern complete +? 0,1,2,3,4,5,6,7 =2 +? Motif name=avlst +? String=avlst +? Minimum matches (1.00-5.00) (5.00) =3 +Probability of score 3.0000 = 0.394E-02 + 1 Exact match +X 2 Percentage match + 3 Cut-off score and score matrix + 4 Cut-off score and weight matrix + 5 Direct repeat + 6 Membership of set + 7 Pattern complete +? 0,1,2,3,4,5,6,7 =7 +? (y/n) (y) Save pattern in a file n + +Pattern description + +Motif 1 named avlst is of class 2 +which is a match of score 3. to the string +avlst +Probability of finding pattern = 0.3941E-02 +Expected number of matches = 0.2030E+01 +? Maximum pattern probability (0.00-1.00) (1.00) = +? Minimum pattern score (-9999.00-9999.00) (-9999.00) = + Select display mode +X 1 Motif by motif + 2 Inclusive + 3 Graphical + 4 EMBL feature table +? 0,1,2,3,4 =4 + Searching + +FT avlst 152 156 Program +Total matches found 1 +Minimum and maximum observed scores 3.00 3.00 + +.end lit +.para +General notes +.para +These methods allow users to define and search for +complex patterns of motifs defined as single objects. +The programs allow individual DNA motifs to be defined in eight +different +ways, and protein motifs in six. Motifs are combined, using the logical +operators AND, OR and NOT, to describe a pattern. The pattern also +specifies the ranges of allowed relative separations of the individual +motifs. +.para +First some definitions. +.para +A MOTIF is a contiguous subsequence of fixed length. +At its simplest +it could be a single definite base or amino acid; a more complex motif +might be better represented as a consensus or a weight matrix; +two more-abstract types of +motif are direct and inverted repeats. +.para +A PATTERN is a higher order of structure defined by a list of motifs. The +motifs in a pattern are combined using the logical operators AND, OR and +NOT. The list also defines the allowed relative separations of the +motifs. In the current versions of the programs up + to 50 motifs can be combined into a single pattern. So using these +definitions there are two +differences between motifs and patterns: 1) the distances between all +elements of a motif are fixed, but +the separations of parts of patterns can vary; + 2) all characters in a motif are defined +using the same method (class), but different parts of a pattern can be +defined in completely different ways. +.para +Each motif +can be represented in 9 ways (known as the motif class): +.sk1 +.lit + MOTIF CLASSES +CLASS DESCRIPTION + 1 Exact match to a short defined sequence. The IUB symbols + can be used for DNA sequences. + 2 Percentage match to a defined short sequence. In nucleic acids, + the IUB symbols can be used. + 3 Match to a defined sequence, using a score matrix and cutoff + score. The DNA matrix (see option 18) gives scores to IUB symbols + depending on their level of redundancy. MDM78 is used for proteins. + 4 Match to a weight matrix with cutoff score. + 5 As class 4 but on the complementary strand. + 6 Inverted repeat or stem-loop. Fixed stem length, range of + loop sizes, and cutoff score using A-T, G-C=2; G-T=1. + 7 Exact match to short sequence but with a defined step size. + 8 Direct repeat. Fixed repeat length, range of loop-out sizes, + cutoff score, and score matrix (for protein sequences MDM78 and + for nucleic acids an identity matrix). + 9 Membership of a set. A list of sets of allowed amino acids for + each position in the motif. The sets are separated by commas(,). + For example IVL,,,DEKR,FYWILVM defines a motif of length 5 amino + acids in which one of I,V or L must be found in the first position, + then anything in the next two positions, D,E,K or R in the fourth + position and F,Y,W,I,L,V or M in the fifth. This class only applies + to protein sequences because for nucleic acids "membership of a +set" + can be achieved using IUB symbols. + + Classes 1 - 4, 8 and 9 apply to protein sequences, and classes 1-8 to + nucleic acids. + +.end lit +.para +Class 1: exact match. +.para +The motif is defined by a short sequence, which for nucleic acids, + may include IUB symbols. All symbols must match. +.para +Class 2: percentage match +.para +The motif is defined by a short sequence, which for nucleic acids, +may include IUB symbols. The minimum number of matching characters +must +also be specified. +.para +Class 3: match using a score matrix +.para +The motif is defined by a short sequence, which for nucleic acids, +may include IUB symbols. The motif is not compared directly with the +sequence to count the number of matching characters. Instead a matrix is +used to provide a score for all possible pairs of characters. The motif +score for +any position along the sequence is the sum of the scores found by +looking-up the scores for each pair of aligned characters. A match is +declared if some minimum score is achieved. +.para +Class 4: weight matrix +.para +The motif is defined by a table of values (called weights or scores). The +table gives a score for finding each possible character at each position +along the length of the motif. It therefore +has dimension motif-length x character-set-size, and allows us to give +different scores for each character at each position. It is equivalent to +having a different score matrix for each position along the motif, and +provides the most flexible and specific method of defining motifs. The +weight matrices are created by program PIP option 20 and +stored as files. The file contains the values +for each position, as well as an overall minimum score. +There are two ways in which these values can be used to calculate an +overall +score for any section of the sequence. The simplest way is to add the +values in the file. (This means that the highest possible score +can be calculated by adding the top value at each column +position, and the lowest +by adding the bottom value.) + The normal way of using the values in the file is as +follows. +First the programs divide the values in each column by the column total +so +that they sum to 1.0 +Then the natural +logs of these values are used as scores. When the matrix is applied to a +sequence these logarithmic values are summed (which is of course +equivalent +to multiplying the frequencies). +Note that using the natural logs of the frequencies as +weights and +adding them means that the overall cutoff score must be less than zero, +whereas if the original +values in the weight matrix file are added, the cutoff score will be +greater than zero. The search routines therefore decide whether the user +wants to add values or multiply frequencies +by examining the value of the cutoff score: it will add if the cutoff +is +greater than zero and add logs of frequencies if it is less than zero. + Hence we effectively get two +motif classes in one. The program PIP, when creating weight matrix +files, will ask the user whether the scores should be added or multiplied. + If the values in the table have been defined +without using a set of aligned sequences +it is easier for the user to +choose a cutoff score if the values are added. +.para +Class 5: complement of weight matrix +.para +The motif is defined by a weight matrix, but the program searches for its +complement. +.para +Class 6: inverted repeat, or stem-loop +.para +The motif is defined by a repeat length, a minimum score + and a range of loop sizes. The scores are A-T=2, G-C=2, G-T=1, else=0. +The loop sizes are defined by a minimum +and maximum distance from the 3' end of the stem. +For a stem-loop these will be positive numbers. For example to +define a stem of length 8 and loop sizes varying from 3 to 5, the stem +would be set to 8, the minimum start distance to 3 and the maximum +to 5. To define an +inverted repeat the minimum distance will be negative. For example stem +length=9, +minimum distance=-9, and maximum distance=-8 will find +inverted repeats of lengths 9 and 10. +E.g. AAAAATTTT and AAAAATTTTT would be found, the first having a base +at +its centre, the second having none. +.para +Class 7: exact match, defined step size. +.para +The motif is defined by a short sequence, which for nucleic acids, + may include IUB symbols. All symbols must match. The class differs +from +class 1 in that searches will move in steps of some given size. For +example +we could search for a certain codon and use a step size of 3 and hence + keep in a +single reading frame. +.para +Class 8: direct repeat +.para +The motif is defined by a repeat length, a minimum score + and a range of loop sizes. The scores are defined using MDM78 for protein +sequences and an identity matrix for nucleic acids. +The loop sizes are defined by a minimum +and maximum distance from the 3' end of the stem. +.para +Class 9: membership of a set +.para +This motif class is for protein sequences. It is defined by lists of +allowed amino acids for each position in the motif, and a cut-off score. +Positions at which any amino acid can occur are left blank. +All allowed amino acids for each position give a score of 1. +The motifs can be defined in two ways: either typed at the keyboard or +read +in as a weight-matrix-like file. +When the motif is defined at the keyboard the sets of allowed amino +acids +are separated by commas(,). + For example IVL,,,DEKR,FYWILVM defines a motif of length 5 amino + acids in which one of I,V or L must be found in the first position, + then anything in the next two positions, D,E,K or R in the fourth + position and F,Y,W,I,L,V or M in the fifth. To specify that the +whole motif must match a score of 3 would be required (i.e. one of the +allowed amino acids must be found for each of the three defined +positions). +If the motif is read from a file the file must have been written by +program +PIP, or have been saved by the pattern searching routines. If the +user +elects to save a pattern, and it includes class 9 motifs typed at the +keyboard, then the program will save the class 9 motifs as weight matrix +files. Therefore it will request file names for each motif of this class. +If the motif given above as an example were saved the weight matrix file +would have 5 columns. +The first column +would contain zeroes except for the I, V and L rows +which would be set to 1; the next two columns would all be zero; the next +would be zero except for the D,E,K and R rows which would be 1; the final +column would contain 1's in rows F,Y,W,I,L,V and M, with +the rest zero. +.para + +The logical operator (AND, OR or NOT) used to add each motif to the +pattern +is specified by preceding +the class number by the letters A, O or N. A = AND, O = OR, N = NOT. +The default is A, so N2 means include, using the NOT operator, a class 2 +motif; O2 means include, using the OR operator, a class 2 motif; both A2 +and +2 mean include, using the AND operator, a class 2 motif. + +.para +Range setting. +.para +The motifs in a pattern are numbered according to their order in the list. +Apart from the first motif in a pattern all motifs are given a range +of allowed positions relative to a motif further up the list. +For example +suppose we have a pattern defined by A AND B AND C AND D. +Motif A can occur anywhere, but B must have its range of allowed +positions defined relative to the position of motif A, and C's positions +can be defined relative to either A or B, depending on which is most +convenient, and likewise D's positions can be relative to A or B or C. +.para +Notice that the positions of motifs can be defined relative to more than +one motif. Suppose we have a pattern consisting of +motifs A, B and C, and that B occurs 5-10 residues right of A, C occurs 5- +10 +residues right of B, and also C is never more than 15 residues from A. +Then +it is quite consistent with the methods to include motif C into the +pattern +twice using the AND operator: once relative to A and once relative to B. +This will define the relative spacing and the ORDER of the motifs in the +pattern. (If we simply defined the position of C relative to A it could be +found to the left of B). +.para +Motifs combined together using the OR operator are all given the same +range. For example suppose we had a pattern A AND (B OR C) AND (D OR E), + then B and C each have the same range, and D and E also have +the same range as one another. The range for D and E can be relative to +A or to B. +.para +Motifs cannot have their ranges defined relative to motifs that are +included using the NOT operator. For example if we had the pattern A NOT +B +AND C, then the range for C can only be defined relative to motif A. +.para +Speed can be gained by arranging the order +of the motifs so that those higher up the list are of types that can be +searched for rapidly and that are also unlikely to be found. +.para +Motifs combined by the OR operator are alternatives: if any one of a set +of motifs +combined by the OR operator is found, then a match is declared. All +alternatives will be reported. For example if we had a pattern defined by +A +AND (B OR C), then all places where A occurs and B is found within range, +and all places where A is found and C is found within range will be +reported. A typical use would be where we might allow a motif to appear +on +either strand of the DNA sequence. For example a weight matrix +representing +the heatshock element could be used in a pattern which included +heatshock +as a motif class 4 combined using the OR operator +with heatshock as a motif class 5. +.para +The probability calculations are performed for each motif as it is +defined. +If an overall probability cut-off is given the calculation is repeated for +each match found. To achieve maximum searching speed do not give an +overall +probability cut-off. Overall cut-off scores should only be used if the +motif +classes used are compatible. +.para +There are currently +several ways to display the matches: 1 = each +motif and its position is listed; 2 = all the sequence between the two +outermost motifs is listed; 3 = graphical, with a spike marking the +position +of the leftmost motif. The library versions also give entry names, and a +one +line title; in addition they can be used to produce aligned families of +sequences. When this mode of output is selected the program will write a +separate file for each match. The files will be called ENTRYNAME.DAT +where +ENTRYNAME is the name of the entry in the library. The matching +sequence +will be written out so that the spacing between motifs is constant, and +set to the maximum allowed by the pattern definition. Any gaps will be +filled with dashes (-). If the individual sequences were subsequently +written one above the other +they should line up so that all motifs are in register. There two types of +output of this sort: one, option 4, writes out whole sequences, the other, +option 5, writes out only the sequences between the two outermost +motifs. +If the individual sequences were subsequently +written one above the other +they should line up so that all motifs are in register. There two types of +output of this sort: one, option 4, writes out whole sequences, the other, +option 5, writes out only the sequences between the two outermost +motifs. +Note that for option 4 users are asked to type the position of the +first motif, and the reason for +this is explained below. +Consider a pattern found in several sequences. Consider only +the first motif in +the pattern and suppose that it was found in different positions in these +sequences. +Say that of these positions the one furthest from the left end was +position 100. Then, in order to ensure that all the sequences would align, +we must specify that motif 1 must start at position 100. +Any sequences in which motif 1 started +nearer to the left end than position 100 would be padded accordingly. +These modes of output +should only be used when the position of each motif is defined relative to +its +immediate neighbour. +.para +The pattern descriptions can be saved to files. These files +can be used instead of typing definitions again at the keyboard. As the +files are annotated, +they can easily +be changed using system editors, and the modified versions used to +define the variant patterns for the programs. +.para +.para +Use of lists of entry names +.para +The two programs that operate on libraries have the ability to +restrict their searches to subsets of the libraries. This does not require +sublibraries to be created but instead is achieved by using files +containing a list of the entry names of sequences. The user may choose to +search only those entries on the list or, alternatively to search all but +those on the list (i.e. in the latter case +the list contains the names of those to be excluded). + The programs can search libraries that have indexes and those that +do not. + If a list of names for inclusion is used, +then the search will be faster if the index is present. In all other +circumstances the whole library will be read. +The list must be in library order except when it is used +to include entries, and an index is available. +The list must contain each entry name on a separate line, with the name +starting in column 1 of the line. ie there must be no spaces at the start +of the line. +The list of entry names +can be produced by the keyword searches of nip, pip, etc as long +as the listings produced have a space character separating the entry name +from the entry description. This will depend on how well the library +reformatting programs work. For example swissprot entry names tend to run +into the beginning of the descriptions, but other libraries are generally +OK. + +.para +One use of the programs is to look for patterns that we already know +about, but in new sequences. However it is hoped that they will also be +useful for finding new motifs. For example +several known control regions in +nucleic acid +sequences consist of particular direct or inverted repeats; +the inclusion of +direct and inverted repeats as motif classes +makes it possible to +find previously unknown +motifs of these types. +Using these new programs we can +ask questions like: "are there any inverted or direct repeats near to +sections of sequence that contain both a +CCAAT box and a TATA box?"; and to search for such things throughout +the +libraries. In addition, the mode of output in which all the sequence +between +the two outermost motifs found is printed out, allows us to extract +sequences and examine them in more detail for further common +subsequences. +For example we might want to collect together all the sequences +between +putative CCAAT and TATA boxes. +.para +A further use of the inverted repeat motif class is the following. If a +regulatory sequence in DNA is poorly defined but also an inverted repeat, +then it might be an advantage to specify it both as a consensus sequence +and +a superimposed inverted repeat. In this way two weak definitions can be +combined to produce a stronger pattern. +.para +Given only a few examples of a motif it +should be possible to perform initial searches using a +class 3 motif, and then, using plausible matching sequences, create a +more +specific weight matrix for the same motif. +.para +If motifs are combined with the first motif using the OR operator +they will be ignored until all +permutations that include the first motif have been looked for. +The whole search will then be repeated, in +turn, for each of +those motifs that are combined with the first motif using the OR +operator. +An interesting consequence of this is that the program can be used, +without +change, to compare any newly determined sequence with all known +individual +motifs. We achieve this by having a pattern in which all known relevant +motifs are combined using the OR operator. +If we ask to use this pattern with +a sequence, the program will automatically compare each individual +motif in +the pattern with the whole length of the +sequence. As the number of known +motifs grows this should become an increasingly useful standard +procedure. +.para +The NOT operator is obviously +useful for making sure particular motifs are not present, but it can also +be used to bracket the levels of matches found. We may want a degree of +match that lies between two limits - binding should occur, but not too +strongly; or base-pairs should form, but not too many. We can specify +this +by asking for a match with a low score, in combination with a match and +a +high score, both for the same motif, but with the high score included +using +the NOT operator. +.para +The algorithm is designed to find all sections of a sequence that satisfy +the pattern rather than only the best match. +Particularly if some of the motifs in a pattern are less well defined than +others, this can often result in the same region of a sequence being +reported as having several matches, but which only vary in the +positions of the weakest motifs. +.para +General remarks on motif searching +.para +Generally motifs are short subsequences that are thought to be +associated with +particular functions in some known sequences. Often +we search for them to try to +understand or interpret other sequences. Sometimes we search for +motifs and +patterns to +test a hypothesis about their role: are they found in the expected +positions in the expected sequences. In doing so we should remember +that, in both proteins and nucleic acids, + what we are really looking for is a particular +three dimensional structure with certain affinities for other structures, +and that we are assuming that the sequence of the motif alone +defines the 3D structure we searching for. + The overall structure +may be completely different to those in which the motif is functional, +and +hence the motif may have a different shape or be inaccessible. +We should be aware of the +importance of the context in which a motif is found. Where does it lie +relative to the overall structure, is it accessible, is the three +dimensional spacing between +it and other motifs correct? For example, is it on the same side of the +double helix, and the correct distance from some other motif? How does +context affect our assessment of the significance of finding a motif? +Finding false mammalian mRNA splice junctions in non-coding sequences +is +far less important than finding false sites in pre-mRNA sequences, but +finding them in the correct places is most important! In other words, it +is +often the case that when we are searching for a motif that is known to +be +necessary for some function, then a positive result in the form of a +match +in the required position, is more important than a high background of +matches in the wrong positions. Being + able to write +down the probability of finding a motif in a random sequence tells us how +well it is defined. +In nucleic +acids the DNA may contain many superimposed types of information such +as +those concerned with histone phasing, protein coding or mRNA secondary +structure. These overlapping "codes" may interfere with one another +causing +matches to motifs to be poorer than expected. +In general we will only have a limited number of examples of the +motif and we do not know how representative they are. +.para +Sequences have superimposed functions: some parts may be of general +structural +importance and give rise to an overall framework, and other parts give +specificity and hence are not common; we may want to use a set of +aligned +sequences to define a motif, but want to use only the framework +positions. + Alternatively we may want to pick out +only those parts of a set of aligned sequences that give a particular +property, and to ignore other similarities that are due to some other +property +and which could obscure the pattern +we are interested in. +It is possible to apply a mask to a set of aligned sequences in +order to give weight to selected positions only. + The ability to define a mask allows certain positions +to be used in the motif and others to be ignored, and yet still permits the +use of a set of aligned sequences to calculate weights. The mask is +requested and applied +by the program and results in the masked positions being zero +in +the weight matrix. The mask is defined in the following way. +Suppose we had a motif of length 15, then the mask +x--x--xx-x will give zero weights to positions 2,3,5,6 and 9 (note it is +the dashes (-) that are significant and that positions +1,4,7,8,10,11,12,13,14 and 15 +will be non-zero). Of course +the same set of sequences could be used with several alternative masks +in +order to extract different features and create corresponding weight +matrices. +.para +The programs are described in Staden,R. +CABIOS 4, 53-60, 1988; Staden,R. + CABIOS 5, 89-96, 1989, anf a forthcoming Methods in +Enzymology. +.left margin1 +@ end of help diff --git a/help/README b/help/README new file mode 100644 index 0000000..b662de2 --- /dev/null +++ b/help/README @@ -0,0 +1,38 @@ + README file for help directory of staden package + ----------------------------------------------- + +Should contain (at least) ProgramName_help where ProgramName is each of +bap, dap, gip, mem, mep, nip, nipf, pip, sap, sip and also staden_help +and stadenp_help. + +There are 3 main formats of file in this directory: + +PROGRAM.RNO: + This is the unformatted (runoff/nroff style) help for PROGRAM. + Any changes to the help should be performed on this file. + +program_help: + This is the online formatted help used by PROGRAM. It can also + be printed to produce hardcopy documentation. + +program_menu: + This is a file that describes the menus used in PROGRAM, + together with an index into the program_help file for the + online help. The format for each line is: + +

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on a graph widget + Last update: Friday 15 May 1992 +*/ + + +/* + `plotEdgeOffset' indicates the pixel position at which point 0 + occurs. The characters for bases are printed with their centres + corresponding to their locations on the plot. + + The cutoffs are actually drawn between the positions of the + bases on either side. + + SD: + Added incScaleFactor, decScaleFactor + Changed scaleTrace to use scale factor + SD: + Support monochrome displays when marking cutoffs + SD: 15-May-1992 + Calculation of next value of pSegN in drawTrace() gives a value one too many + +*/ + + + + +/* ---- Includes ---- */ + +#include "traceDisplay.h" +#include "display.h" /* IMPORT: userEvent */ +#include "Graph.h" +#include "seq.h" /* IMPORT: Seq, NULLSeq, getNPoints */ + + +#include +#include +#include + + + +/* ---- Statics ---- */ + +static Seq currSeq = NULLSeq; +static int NPoints; +static TRACE maxTraceVal; + +static Widget traceWid; + +static Dimension traceWidth; /* Current width of the trace graph */ +static Dimension traceHeight; /* Current height of the trace graph */ +static Dimension plotEdgeOffset; +static Dimension plotWidth; /* Current width of the plot + = graphWidth - 2*plotEdgeOffset */ + +static int leftCutoff, rightCutoff; + +static GC Agc, Cgc, Ggc, Tgc; +static Pixel normBackground, dimBackground; + +/* monochrome stippling */ +static GC greygc; +static int planes; + +static float scaleFactor; + + + + +/* ---- Position translation ---- */ + +/* + Convert a value from the `point-in-the-sequence' to the + `x-pixel-on-the-graph' scale, and vice-versa, given that + `plotWidth' holds the current pixel width of the graph which + starts `plotEdgeOffset' from either edge of the graph and + `NPoints' gives the current point width. + Fractional results are truncated - for pixelToPoint this may + mean a point one too low is indicated, but a point too high is + never indicated. +*/ + + +static int pointToPixel(int point) +{ return(((point * plotWidth) / (NPoints-1)) + plotEdgeOffset); +} + + +static int pixelToPoint(int pixel) +{ return(((pixel-plotEdgeOffset) * (NPoints-1)) / plotWidth); +} + + +static int scaleTrace(int y) +{ return(traceHeight - + (int)(scaleFactor * (float)y * (float)traceHeight / (float)maxTraceVal) + ); +} + + +static int leftCutoffPoint(int baseNum) +/* + Return the point corresponding to a left cutoff at `baseNum'. +*/ +{ int pL, pR; + + pL = (baseNum == 0) ? 0 : baseNumToPoint(currSeq, EdBases, baseNum-1); + pR = (baseNum == 0) ? 0 : baseNumToPoint(currSeq, EdBases, baseNum); + + return((pR==NULLPoint)?pL:(pL+pR)/2); +} + + +static int rightCutoffPoint(int baseNum) +/* + Return the point corresponding to a right cutoff at `baseNum'. +*/ +{ int NedBases = getNBases(currSeq, EdBases); + int pL, pR; + + pL = (baseNum == 0) + ? NPoints-1 + : baseNumToPoint(currSeq, EdBases, NedBases-baseNum); + pR = (baseNum == 0) + ? NPoints-1 + : baseNumToPoint(currSeq, EdBases, NedBases-baseNum-1); + + return((pL+pR)/2); +} + + + + + +/* ---- Internal routines ---- */ + + + + +static void drawTrace(Widget wid, char base, int p0, int pN) +/* + Draw the `base' trace between points p0..pN (inclusive) +*/ +{ GC gc; + int pSeg, pSeg0, pSegN; +#define NSegs 500 + int traces[NSegs]; + XPoint xPoint[NSegs]; + + switch (base) + { case 'A': gc=Agc; break; + case 'C': gc=Cgc; break; + case 'G': gc=Ggc; break; + case 'T': gc=Tgc; break; + } + + + /* + The range (p0..pN) is drawn as a series of overlapping + segments (pSeg0..pSegN). + */ + pSegN = p0; + do + { int i; + + /* + This segment carries on from and including the last point + of the last segment. + */ + pSeg0 = pSegN; + pSegN = ((pSeg0+NSegs-1)<=pN) ? pSeg0+NSegs-1 : pN; + /* Get the traces for this segment */ + getTraces(currSeq, base, pSeg0, pSegN, traces); + + /* Translate to pixel coords and place in an XPoint array */ + pSeg = pSeg0; + i = 0; + while (pSeg<=pSegN) + { xPoint[i].x = pointToPixel(pSeg); + xPoint[i].y = scaleTrace(traces[i]); + pSeg++; + i++; + } + + /* Draw the segments in the XPoint array */ + XDrawLines(XtDisplay(wid), + XtWindow(wid), + gc, + xPoint, + pSegN-pSeg0+1, + CoordModeOrigin); + } while (pSegN < pN); +} + + + + + +/* ---- Callbacks ---- */ + + + + +static void exposeCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ Region region = (Region) call_data; + XRectangle rect; + int x0, xN; /* Affected region: pixels */ + int p0, pN; /* Affected region: points */ + int leftCutoffP, rightCutoffP; + + + if ((currSeq == NULLSeq) || isBasesOnly(currSeq)) return; + + + /* + `region' gives us the exposed graph region. + XClipBox will return a bounding rectangle, in pixels. + */ + XClipBox(region, &rect); + x0 = rect.x; + xN = rect.x+rect.width; + + /* Trim it so we only consider the plot proper */ + if ((x0>plotWidth+plotEdgeOffset) || (xNplotWidth+plotEdgeOffset) ? plotWidth+plotEdgeOffset : xN; + + /* Convert to affected points */ + p0 = pixelToPoint(x0); + pN = pixelToPoint(xN); + if (pNrightCutoffP) + /* + Some of the exposed region is in the right cutoff area. + Repaint it with dimmed background. + */ + { int firstP = (p0>rightCutoffP) ? p0 : rightCutoffP; + int lastP = pN; + + int pix0 = pointToPixel(firstP); + int pixN = pointToPixel(lastP); + + if (planes == 1) { + XFillRectangle(XtDisplay(traceWid), XtWindow(traceWid), + greygc, + pix0, 0, + pixN-pix0, traceHeight); + } else { + XSetWindowBackground(XtDisplay(traceWid), XtWindow(traceWid), + dimBackground); + XClearArea(XtDisplay(traceWid), XtWindow(traceWid), + pix0, 0, + pixN-pix0, traceHeight, + False); + XSetWindowBackground(XtDisplay(traceWid), XtWindow(traceWid), + normBackground); + } + } + + /* + Now draw the traces. + The GC background is unused when drawing lines, so we do + not need to change it depending on whether we are in the + cutoff region or not. + */ + drawTrace(wid, 'A', p0, pN); + drawTrace(wid, 'C', p0, pN); + drawTrace(wid, 'G', p0, pN); + drawTrace(wid, 'T', p0, pN); +} + + + + +static void userCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +/* + Handles all XtNcallbacks, i.e., buttons and keys. +*/ +{ XEvent *eventP = (XEvent *) call_data; + + if (currSeq == NULLSeq) return; + + /* + Callback to the `display' module. + */ + userEvent(eventP); +} + + + + +static void resizeCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ Arg args[10]; + int nargs; + + /* + This routine can be called in two ways. + (a) as a callback when the trace is resized by the user + (b) indirectly when setGraphWidth alters the graph width + */ + nargs = 0; + XtSetArg(args[nargs], XtNheight, &traceHeight); nargs++; + XtSetArg(args[nargs], XtNwidth, &traceWidth); nargs++; + XtGetValues(wid, args, nargs); + + if (XtIsRealized(wid)) XClearWindow(XtDisplay(wid), XtWindow(wid)); +} + + + + +/* ---- Exports ---- */ + + +void redisplayTraces() +{ + /* + ** Clear the area and generate an expose. + */ + XClearArea(XtDisplay(traceWid), XtWindow(traceWid), + 0, 0, + 0, 0, + True); +} + +void incScaleFactor() +{ + scaleFactor *= 1.5; + redisplayTraces(); +} + +void decScaleFactor() +{ + scaleFactor /= 1.5; + redisplayTraces(); +} + +void setScaleFactor(float sf) +{ + scaleFactor = sf; + redisplayTraces(); +} + + + +void createTraceDisplay(Widget parentWid, Widget fromVertWid, + Dimension width, Dimension offset) +/* + Create the trace display within the Form widget `parent', + with the XtNfromVert constraint `fromVertWid'. The initial + width is `width' and the plot is drawn `offset' from + the left and right hand edges. + No trace is initially displayed. +*/ +{ Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNwidth, width); nargs++; + traceWid = XtCreateManagedWidget("trace", graphWidgetClass, + parentWid, args, nargs); + XtAddCallback(traceWid, XtNcallback, userCallback, NULL); + XtAddCallback(traceWid, XtNexposeCallback, exposeCallback, NULL); + XtAddCallback(traceWid, XtNresizeCallback, resizeCallback, NULL); + + nargs = 0; + XtSetArg(args[nargs], XtNheight, &traceHeight); nargs++; + XtGetValues(traceWid, args, nargs); + traceWidth = width; + plotWidth = traceWidth-(2*plotEdgeOffset); + plotEdgeOffset = offset; + scaleFactor = 1.0; + + currSeq = NULLSeq; + + /* + Get the GCs + */ + Agc = GraphGC1(traceWid); + Cgc = GraphGC2(traceWid); + Ggc = GraphGC3(traceWid); + Tgc = GraphGC4(traceWid); + + /* + Get the backgrounds + */ + nargs = 0; + XtSetArg(args[nargs], XtNbackground, &normBackground); nargs++; + XtSetArg(args[nargs], XtNdimBackground, &dimBackground); nargs++; + XtGetValues(traceWid, args, nargs); + + planes = DisplayPlanes(XtDisplay(traceWid),DefaultScreen(XtDisplay(traceWid))); + if (planes == 1) { + /* prepare for monochrome display */ + XtGCMask valuemask = (GCStipple | GCFillStyle); + XGCValues values; + Pixmap grey_stipple; + +#define grey_width 4 +#define grey_height 4 + static char grey_bits[] = { + 0x0e, 0x0b, 0x0e, 0x0b}; + + grey_stipple = + XCreateBitmapFromData(XtDisplay(traceWid), + RootWindowOfScreen(XtScreenOfObject(traceWid)), + grey_bits, + grey_width, + grey_height); + + values.stipple = grey_stipple; + values.fill_style = FillOpaqueStippled; + greygc = XCreateGC(XtDisplayOfObject(traceWid), + RootWindowOfScreen(XtScreenOfObject(traceWid)), + valuemask, &values); + + } + +} + + + + +void displayTrace(Seq seq) +/* + Display the trace of sequence `seq'. +*/ +{ + if (seq == NULLSeq) return; + currSeq = seq; + + + /* + Get static information about the current sequence. + */ + NPoints = getNPoints(currSeq); + maxTraceVal = getMaxTraceVal(currSeq); + getCutoffs(currSeq, &leftCutoff, &rightCutoff); +} + + + + +void unDisplayTrace() +/* + Cease displaying the current sequence (if any). +*/ +{ currSeq = NULLSeq; +} + + + + +void setTraceWidth(Dimension width) +/* + Set the width of the current sequence trace display. +*/ +{ Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNwidth, width); nargs++; + XtSetValues(traceWid, args, nargs); + + traceWidth = width; + plotWidth = traceWidth-(2*plotEdgeOffset); + + /* + Clear the graph. + */ + if (XtIsRealized(traceWid)) + XClearWindow(XtDisplay(traceWid), XtWindow(traceWid)); +} + + + + +void traceCutoffChange() +/* + One or both of the cutoffs have changed. + Update our display. +*/ +{ int newLeftCutoff, newRightCutoff; + + getCutoffs(currSeq, &newLeftCutoff, &newRightCutoff); + + + if (newLeftCutoff != leftCutoff) + /* + The region between newLeftCutoff and leftCutoff has changed. + */ + { int b0, bN; + int p0, pN; + int pix0, pixN; + + /* + b0 = leftmost base number. bN = rightmost base number. + */ + if (newLeftCutoff /* IMPORT: Widget, Dimension */ + + + + +/* ---- Exports ---- */ + + +extern void createTraceDisplay(Widget parentWid, Widget fromVertWid, + Dimension width, Dimension offset); +/* + Create the trace display within the Form widget `parent', + with the XtNfromVert constraint `fromVertWid'. The initial + width is `width' and the plot is drawn `plotEdgeOffset' from + the left and right hand edges. + No trace is initially displayed. +*/ + + +extern void displayTrace(Seq seq); +/* + Display the trace of sequence `seq'. +*/ + + +extern void unDisplayTrace(); +/* + Cease displaying the trace of the current sequence (if any). +*/ + + +extern void setTraceWidth(Dimension width); +/* + Set the width of the current sequence trace display. +*/ + + +extern void traceCutoffChange(); +/* + One or both of the cutoffs have changed. + Update our display. +*/ + + +extern void incScaleFactor(); +/* + Increment vertical scaling of trace +*/ + + +extern void decScaleFactor(); +/* + Decrement vertical scaling of trace +*/ + + + +extern void setScaleFactor(float sf); +/* + Set vertical scaling of trace +*/ diff --git a/src/ted/values.h b/src/ted/values.h new file mode 100644 index 0000000..792e966 --- /dev/null +++ b/src/ted/values.h @@ -0,0 +1,118 @@ +/* @(#)values.h 1.7 89/10/02 SMI; from S5R2 1.16 */ + +#ifndef _values_h +#define _values_h + +/* These values work with any binary representation of integers + * where the high-order bit contains the sign. */ + +/* a number used normally for size of a shift */ +#if gcos +#define BITSPERBYTE 9 +#else +#define BITSPERBYTE 8 +#endif +#define BITS(type) (BITSPERBYTE * (int)sizeof(type)) + +/* short, regular and long ints with only the high-order bit turned on */ +#define HIBITS ((short)(1 << BITS(short) - 1)) +#define HIBITI (1 << BITS(int) - 1) +#define HIBITL (1L << BITS(long) - 1) + +/* largest short, regular and long int */ +#define MAXSHORT ((short)~HIBITS) +#define MAXINT (~HIBITI) +#define MAXLONG (~HIBITL) + +/* various values that describe the binary floating-point representation + * _EXPBASE - the exponent base + * DMAXEXP - the maximum exponent of a double (as returned by frexp()) + * FMAXEXP - the maximum exponent of a float (as returned by frexp()) + * DMINEXP - the minimum exponent of a double (as returned by frexp()) + * FMINEXP - the minimum exponent of a float (as returned by frexp()) + * MAXDOUBLE - the largest double + ((_EXPBASE ** DMAXEXP) * (1 - (_EXPBASE ** -DSIGNIF))) + * MAXFLOAT - the largest float + ((_EXPBASE ** FMAXEXP) * (1 - (_EXPBASE ** -FSIGNIF))) + * MINDOUBLE - the smallest double (_EXPBASE ** (DMINEXP - 1)) + * MINFLOAT - the smallest float (_EXPBASE ** (FMINEXP - 1)) + * DSIGNIF - the number of significant bits in a double + * FSIGNIF - the number of significant bits in a float + * DMAXPOWTWO - the largest power of two exactly representable as a double + * FMAXPOWTWO - the largest power of two exactly representable as a float + * _IEEE - 1 if IEEE standard representation is used + * _DEXPLEN - the number of bits for the exponent of a double + * _FEXPLEN - the number of bits for the exponent of a float + * _HIDDENBIT - 1 if high-significance bit of mantissa is implicit + * LN_MAXDOUBLE - the natural log of the largest double -- log(MAXDOUBLE) + * LN_MINDOUBLE - the natural log of the smallest double -- log(MINDOUBLE) + * LN_MAXFLOAT - the natural log of the largest float + * LN_MINFLOAT - the natural log of the smallest float + */ +#if u3b || u3b5 || sun +#define MAXDOUBLE 1.797693134862315708e+308 +#define MAXFLOAT ((float)3.40282346638528860e+38) +#define MINDOUBLE 4.94065645841246544e-324 +#define MINFLOAT ((float)1.40129846432481707e-45) +#define _IEEE 1 +#define _DEXPLEN 11 +#define _HIDDENBIT 1 +#define DMINEXP (-(DMAXEXP + DSIGNIF - _HIDDENBIT - 3)) +#define FMINEXP (-(FMAXEXP + FSIGNIF - _HIDDENBIT - 3)) +#define LN_MAXFLOAT 8.8722839052068e+01 +#define LN_MINFLOAT -1.03278929903432e+02 +#endif +#if pdp11 || vax +#define MAXDOUBLE 1.701411834604692293e+38 +#define MAXFLOAT ((float)1.701411733192644299e+38) +/* The following is kludged because the PDP-11 compilers botch the simple form. + The kludge causes the constant to be computed at run-time on the PDP-11, + even though it is still "folded" at compile-time on the VAX. */ +#define MINDOUBLE (0.01 * 2.938735877055718770e-37) +#define MINFLOAT ((float)MINDOUBLE) +#define _IEEE 0 +#define _DEXPLEN 8 +#define _HIDDENBIT 1 +#define DMINEXP (-DMAXEXP) +#define FMINEXP (-FMAXEXP) +#endif +#if gcos +#define MAXDOUBLE 1.7014118346046923171e+38 +#define MAXFLOAT ((float)1.7014118219281863150e+38) +#define MINDOUBLE 2.9387358770557187699e-39 +#define MINFLOAT ((float)MINDOUBLE) +#define _IEEE 0 +#define _DEXPLEN 8 +#define _HIDDENBIT 0 +#define DMINEXP (-(DMAXEXP + 1)) +#define FMINEXP (-(FMAXEXP + 1)) +#endif +#if u370 +#define _LENBASE 4 +#else +#define _LENBASE 1 +#endif +#define _EXPBASE (1 << _LENBASE) +#define _FEXPLEN 8 +#define DSIGNIF (BITS(double) - _DEXPLEN + _HIDDENBIT - 1) +#define FSIGNIF (BITS(float) - _FEXPLEN + _HIDDENBIT - 1) +#define DMAXPOWTWO ((double)(1L << BITS(long) - 2) * \ + (1L << DSIGNIF - BITS(long) + 1)) +#define FMAXPOWTWO ((float)(1L << FSIGNIF - 1)) +#define DMAXEXP ((1 << _DEXPLEN - 1) - 1 + _IEEE) +#define FMAXEXP ((1 << _FEXPLEN - 1) - 1 + _IEEE) +#define LN_MAXDOUBLE (M_LN2 * DMAXEXP) +#define LN_MINDOUBLE (M_LN2 * (DMINEXP - 1)) + +#define H_PREC (DSIGNIF % 2 ? (1L << DSIGNIF/2) * M_SQRT2 : 1L << DSIGNIF/2) +#define X_EPS (1.0/H_PREC) +#define X_PLOSS ((double)(long)(M_PI * H_PREC)) +#define X_TLOSS (M_PI * DMAXPOWTWO) +#define M_LN2 0.69314718055994530942 +#define M_PI 3.14159265358979323846 +#define M_SQRT2 1.41421356237309504880 +#define MAXBEXP DMAXEXP /* for backward compatibility */ +#define MINBEXP DMINEXP /* for backward compatibility */ +#define MAXPOWTWO DMAXPOWTWO /* for backward compatibility */ + +#endif /*!_values_h*/ diff --git a/src/update_subclones/README b/src/update_subclones/README new file mode 100644 index 0000000..e59522b --- /dev/null +++ b/src/update_subclones/README @@ -0,0 +1,25 @@ +Software to Maintain Subclone Library Database +---------------------------------------------- + +The subclone library database stores information on sublcone libraries. +For the syntax of this file (subclones) refer to the file +subclones.syntax. + +update_subclones + Programs that prompts the user for information to be added to + the subclone library database. Ensure that the owner of + update_subclones is the same as that of subclones, and that the + setuid bit is set. + +update_subclones.c + The source code for subclonedb and update_subclones + +format.awk + An attempt to write an awk script to produce output for humans + to read. It produces a tbl format file. To use type: + + awk -f format.awk subclones | tbl | ptroff -t >! out.ps + +subclones + The subclone library database + diff --git a/src/update_subclones/format.awk b/src/update_subclones/format.awk new file mode 100644 index 0000000..82f81e5 --- /dev/null +++ b/src/update_subclones/format.awk @@ -0,0 +1,57 @@ +BEGIN { + entries_per_page = 50; + PP = 0; +} +{ + if ( (NR % entries_per_page) == 1 ) { + if (PP) print ".bp"; + PP++; + print "Page - " PP; + # + print ".sp 3"; + print ".TS"; + print "box, tab (%);"; + print "c s s s s"; + print " c | c | c | c | c | c"; + print " c | c | c | c | c | c"; + print " l | l | l | l | l | l."; + print "Cosmid Subclone Libraries"; + print "="; + print "Microtitre%Cosmid%Size Range%Fragmentation%Vector%Comments" ; + print "Dish Number%%(bases)%Method%"; + print "="; + } + + if (NF > 5) { + line = $0; + s = index(line,"("); + t = index(line,")"); + count = 0; + while (s!=0 && t!=0) { + comment = substr(line,s+1,t-s-1); + line = substr(line,t+1); + if (count==0) { + print $1 "%" $2 "%" $3 "%" $4 "%" $5 "%" comment; + } else { + print "%%%%%" comment; + } + count++; + s = index(line,"("); + t = index(line,")"); + } + if (count>1) print "%%%%%_"; + } else + print $1 "%" $2 "%" $3 "%" $4 "%" $5 "%"; + + if ( (NR % entries_per_page) == 0 ) { + print ".TE"; + } +} + +END { + if ( (NR % entries_per_page) != 0 ) { + # fill up page + for(i=NR; i % entries_per_page; i++) print "%%%%%"; + print ".TE"; + } +} diff --git a/src/update_subclones/makefile-alpha b/src/update_subclones/makefile-alpha new file mode 100644 index 0000000..7f1782d --- /dev/null +++ b/src/update_subclones/makefile-alpha @@ -0,0 +1,42 @@ +# +# Makefile for subclonedb +# + +# +# Programs made by this makefile +# +PROGS = update_subclones + +# +# Where the wild things are +# +SRC = . +MISCSRC = $(SRC)/../Misc +MISCBIN = $(MISCSRC)/alpha-binaries +INCLUDES = -I$(SRC) -I$(MISCSRC) + +# +# Alpha +# +CC = cc +CFLAGS = -g -std1 -ieee_with_inexact $(INCLUDES) +LD = cc +LDFLAGS = -g -non_shared + +# +# Here they are +# + +update_subclones : update_subclones.o $(MISCBIN)/misc.a + $(LD) $(LDFLAGS) -o update_subclones update_subclones.o $(MISCBIN)/misc.a + +# +# Special dependencies +# +all: $(PROGS) + +clean: + /bin/rm -f $(PROGS) + +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< diff --git a/src/update_subclones/makefile-dec b/src/update_subclones/makefile-dec new file mode 100644 index 0000000..280e437 --- /dev/null +++ b/src/update_subclones/makefile-dec @@ -0,0 +1,42 @@ +# +# Makefile for subclonedb +# + +# +# Programs made by this makefile +# +PROGS = subclonedb update_subclones + +# +# Where the wild things are +# +SRC = . +MISCSRC = $(SRC)/../Misc +MISCBIN = $(MISCSRC) +INCLUDES = -I$(SRC) -I$(MISCSRC) + +# +# Sun +# +CC = c89 +CFLAGS = -common -g $(INCLUDES) + +# +# Here they are +# +subclonedb : $(SRC)/subclonedb.c $(MISCBIN)/misc.a + $(CC) $(CFLAGS) -o subclonedb $(SRC)/subclonedb.c $(MISCBIN)/misc.a + +update_subclones : $(SRC)/update_subclones.c $(MISCBIN)/misc.a + $(CC) $(CFLAGS) -o update_subclones $(SRC)/update_subclones.c $(MISCBIN)/misc.a + +# +# Special dependencies +# +all: $(PROGS) + +clean: + /bin/rm -f $(PROGS) + +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< diff --git a/src/update_subclones/makefile-sgi b/src/update_subclones/makefile-sgi new file mode 100644 index 0000000..1b00648 --- /dev/null +++ b/src/update_subclones/makefile-sgi @@ -0,0 +1,44 @@ +# +# Makefile for subclonedb +# + +# +# Programs made by this makefile +# +PROGS = subclonedb update_subclones + +SHELL=/bin/sh + +# +# Where the wild things are +# +SRC = . +MISCSRC = $(SRC)/../Misc +MISCBIN = $(MISCSRC) +INCLUDES = -I$(SRC) -I$(MISCSRC) + +# +# SGI +# +CC = cc +CFLAGS = -g $(INCLUDES) + +# +# Here they are +# +subclonedb : $(SRC)/subclonedb.c $(MISCBIN)/misc.a + $(CC) $(CFLAGS) -o subclonedb $(SRC)/subclonedb.c $(MISCBIN)/misc.a + +update_subclones : $(SRC)/update_subclones.c $(MISCBIN)/misc.a + $(CC) $(CFLAGS) -o update_subclones $(SRC)/update_subclones.c $(MISCBIN)/misc.a + +# +# Special dependencies +# +all: $(PROGS) + +clean: + /bin/rm -f $(PROGS) + +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< diff --git a/src/update_subclones/makefile-solaris b/src/update_subclones/makefile-solaris new file mode 100644 index 0000000..b9d66dc --- /dev/null +++ b/src/update_subclones/makefile-solaris @@ -0,0 +1,42 @@ +# +# Makefile for subclonedb +# + +# +# Programs made by this makefile +# +PROGS = subclonedb update_subclones + +# +# Where the wild things are +# +SRC = . +MISCSRC = $(SRC)/../Misc +MISCBIN = $(MISCSRC) +INCLUDES = -I$(SRC) -I$(MISCSRC) + +# +# Sun +# +CC = gcc +CFLAGS = -g $(INCLUDES) + +# +# Here they are +# +subclonedb : $(SRC)/subclonedb.c $(MISCBIN)/misc.a + $(CC) $(CFLAGS) -o subclonedb $(SRC)/subclonedb.c $(MISCBIN)/misc.a + +update_subclones : $(SRC)/update_subclones.c $(MISCBIN)/misc.a + $(CC) $(CFLAGS) -o update_subclones $(SRC)/update_subclones.c $(MISCBIN)/misc.a + +# +# Special dependencies +# +all: $(PROGS) + +clean: + /bin/rm -f $(PROGS) + +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< diff --git a/src/update_subclones/makefile-sun b/src/update_subclones/makefile-sun new file mode 100644 index 0000000..b9d66dc --- /dev/null +++ b/src/update_subclones/makefile-sun @@ -0,0 +1,42 @@ +# +# Makefile for subclonedb +# + +# +# Programs made by this makefile +# +PROGS = subclonedb update_subclones + +# +# Where the wild things are +# +SRC = . +MISCSRC = $(SRC)/../Misc +MISCBIN = $(MISCSRC) +INCLUDES = -I$(SRC) -I$(MISCSRC) + +# +# Sun +# +CC = gcc +CFLAGS = -g $(INCLUDES) + +# +# Here they are +# +subclonedb : $(SRC)/subclonedb.c $(MISCBIN)/misc.a + $(CC) $(CFLAGS) -o subclonedb $(SRC)/subclonedb.c $(MISCBIN)/misc.a + +update_subclones : $(SRC)/update_subclones.c $(MISCBIN)/misc.a + $(CC) $(CFLAGS) -o update_subclones $(SRC)/update_subclones.c $(MISCBIN)/misc.a + +# +# Special dependencies +# +all: $(PROGS) + +clean: + /bin/rm -f $(PROGS) + +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< diff --git a/src/update_subclones/subclonedb.c b/src/update_subclones/subclonedb.c new file mode 100644 index 0000000..ae9175f --- /dev/null +++ b/src/update_subclones/subclonedb.c @@ -0,0 +1,415 @@ +#include +#define USER_DATA +#ifdef USER_DATA +#include +#endif +#include "misc.h" /* IMPORT: is_file */ + +/* +** This is an incredible hack. The original is based on a csh script +** Beware of horrid goto's etc +*/ +typedef char String[200]; +String SUBCLONES; /* initialised in initialise() */ +String methods[] = { + "shear", + "Sau3A", + "Alu-1", +}; +String vectors[] = { + "M13", + "pBS", + "pEMBL9", + "pUC118", +}; +String sizes[] = { + "1-2", + "6-9", + "6-9", + "9-14", +}; +int MATCH = 3; + +/* +** Defaults +*/ +String def_mtd; +String def_cosmid; +String def_method; +String def_vector; +String def_size; +String def_verify; + +/* +** Answers +*/ +String ans_mtd; +String ans_cosmid; +String ans_method; +String ans_vector; +String ans_size; +#define MAX_COMMENTS 10 +String ans_comments[MAX_COMMENTS]; +int comments; +String ans_verify; + +static void initialise() +/* +** Initialise miscellaneous variables +*/ +{ + char *subclones; + + /* clonelib = subclones:$SUBCLONES */ + if (is_file("subclones")) + strcpy(SUBCLONES,"subclones"); + else { + subclones = (char *) getenv("SUBCLONES"); + if (subclones == NULL) + SUBCLONES[0] = '\0'; + else if (is_file(subclones)) + strcpy(SUBCLONES,subclones); + else + SUBCLONES[0] = '\0'; + } +} + +int set_defaults() +{ + FILE *f; + String line; + String last_cosmid; + String max_mtd; + String mtd; + String alpha; + int number; + int i; + + /* Determine SUBCLONES */ + initialise(); + + if ( (f = fopen(SUBCLONES,"r")) == NULL ) return 1; + + max_mtd[0] = '\0'; + while ( fgets(line,sizeof(line),f) ) { + sscanf(line, "%s %s", mtd,last_cosmid); + if (strcmp(max_mtd,mtd) < 0) strcpy(max_mtd,mtd); + } + + if ( ! max_mtd[0] ) return 1; + + /* + ** generate next number + */ + for (i = 0;isalpha(max_mtd[i]);i++) alpha[i] = max_mtd[i]; + alpha[i] = '\0'; + number = atoi(&max_mtd[i]) + 1; + + sprintf(def_mtd,"%s%02d",alpha,number); + strcpy(def_cosmid, last_cosmid); + strcpy(def_method, methods[0]); + strcpy(def_vector, vectors[0]); + strcpy(def_size, sizes[0]); + strcpy(def_verify, "Yes"); + + return 0; +} + + + +ask_mtd() +{ + ask: + printf("Microtitre dish [%s] ? ",def_mtd); + gets(ans_mtd); + + if (strcmp(ans_mtd,"?")==0) { + printf("* Enter the name of the new microtitre dish to be added to the library.\n"); + printf(" A microtitre dish must be a letter followed by two digits. Eg a87\n"); + goto ask; + } else if (ans_mtd[0]) { + if (! islower(ans_mtd[0]) || + (strlen(ans_mtd)==3 ? + ! isdigit(ans_mtd[1]) : + ! islower(ans_mtd[1])) || + ! isdigit(ans_mtd[2]) || + (strlen(ans_mtd)==3 ? + ans_mtd[3] : + ! isdigit(ans_mtd[3]) || + ans_mtd[4])) { + printf("! Invalid microtitre dish name.\n"); + printf(" A microtitre dish must be a letter followed by two digits. Eg a87\n"); + goto ask; + } + } else { + strcpy(ans_mtd,def_mtd); + } + +} + + + + +ask_cosmid() +{ + ask: + + printf ("Cosmid [%s] ? ",def_cosmid); + gets(ans_cosmid); + + if ( strcmp(ans_cosmid,"?") == 0 ) { + printf("* Enter the name of the cosmid from which the subclones was made.\n"); + printf(" NO check is made on the correctness of the value entered.\n"); + goto ask; + } else if ( ans_cosmid[0] ){ + int i; + for (i=0; ipw_name); + shell_call("hostname",output, sizeof(output)); + fprintf(f," %s",output); + shell_call("date",output, sizeof(output)); + fprintf(f," %s",output); +#endif + + fprintf(f,"\n"); + fclose(f); + return 0; +} + + + + +main () +{ + + + printf("Maintain subclone database\n\n"); + + if ( set_defaults() ) { + fprintf (stderr,"Cannot open file %s\n",SUBCLONES); + exit(1); + } + + printf("Database=%s\n\n",SUBCLONES); + + ask_mtd(); + ask_cosmid(); + ask_method(); + ask_vector(); + ask_size(); + ask_comments(); + + ask_verify(); + + if ( strchr("Yy",ans_verify[0]) != NULL) { + printf("Updating file %s with new values...",SUBCLONES); + update(); + printf("done\n"); + } else { + printf("No update made\n"); + } + + +} diff --git a/src/update_subclones/subclones.syntax b/src/update_subclones/subclones.syntax new file mode 100644 index 0000000..5eba956 --- /dev/null +++ b/src/update_subclones/subclones.syntax @@ -0,0 +1,37 @@ +Cosmid Subclone Libraries + +The cosmid subclone library is a file of the following format: + + ::= | + ::= + + + + + + + ::= + ::= + ::= .. + ::= + ::= + ::= | + ::= ( ) + ::= | + ::= # + +where: + + ::= any series of characters terminated by white space + ::= integer number + ::= user id of person who created entry + ::= from where entry was created + ::= date the entry was created + + +Here (at MRC): + + ::= + + + diff --git a/src/update_subclones/update_subclones.c b/src/update_subclones/update_subclones.c new file mode 100644 index 0000000..865ffc3 --- /dev/null +++ b/src/update_subclones/update_subclones.c @@ -0,0 +1,427 @@ +#include +/*#define USER_DATA*/ +#ifdef USER_DATA +#include +#endif +#include "misc.h" /* IMPORT: is_file */ + +/* +** This is an incredible hack. The original is based on a csh script +** Beware of horrid goto's etc +*/ +typedef char String[200]; +String SUBCLONES; /* initialised in initialise() */ +String methods[] = { + "shear", + "Sau3A", + "Alu-1", +}; +String vectors[] = { + "M13mp18", + "pBS", + "pEMBL9", + "pUC118", +}; +String sizes[] = { + "1000..2000", + "6000..9000", + "6000..9000", + "9000..14000", +}; +int MATCH = 3; + +/* +** Defaults +*/ +String def_mtd; +String def_cosmid; +String def_method; +String def_vector; +String def_size; +String def_verify; + +/* +** Answers +*/ +String ans_mtd; +String ans_cosmid; +String ans_method; +String ans_vector; +String ans_size; +#define MAX_COMMENTS 10 +String ans_comments[MAX_COMMENTS]; +int comments; +String ans_verify; + +static void initialise() +/* +** Initialise miscellaneous variables +*/ +{ + char *subclones; + + /* clonelib = subclones:$SUBCLONES */ + if (is_file("subclones")) + strcpy(SUBCLONES,"subclones"); + else { + subclones = (char *) getenv("SUBCLONES"); + if (subclones == NULL) + SUBCLONES[0] = '\0'; + else if (is_file(subclones)) + strcpy(SUBCLONES,subclones); + else + SUBCLONES[0] = '\0'; + } +} + +int set_defaults() +{ + FILE *f; + String line; + String last_cosmid; + String max_mtd; + String mtd; + String alpha; + int number; + int i; + + /* Determine SUBCLONES */ + initialise(); + + if ( (f = fopen(SUBCLONES,"r")) == NULL ) return 1; + + max_mtd[0] = '\0'; + while ( fgets(line,sizeof(line),f) ) { + sscanf(line, "%s %s", mtd,last_cosmid); + if (strcmp(max_mtd,mtd) < 0) strcpy(max_mtd,mtd); + } + + if ( ! max_mtd[0] ) return 1; + + /* + * generate next number: + * + * ::= + * ::= | | + * ::= [a-z] + * ::= [0-9] + * ::= + */ + { + int j = -1; + for (i = 0;isalnum(max_mtd[i]);i++) { + if (isalpha(max_mtd[i])) j = i; + alpha[i] = max_mtd[i]; + } + alpha[++j] = '\0'; + number = atoi(&max_mtd[j]) + 1; + } + + sprintf(def_mtd,"%s%02d",alpha,number); + strcpy(def_cosmid, last_cosmid); + strcpy(def_method, methods[0]); + strcpy(def_vector, vectors[0]); + strcpy(def_size, sizes[0]); + strcpy(def_verify, "Yes"); + + return 0; +} + + + +ask_mtd() +{ + ask: + printf("Microtitre dish [%s] ? ",def_mtd); + gets(ans_mtd); + + if (strcmp(ans_mtd,"?")==0) { + printf("* Enter the name of the new microtitre dish to be added to the library.\n"); + printf(" A microtitre dish must be a letter followed by two digits,\n with a possible alphanumeric prefix. Eg a87\n"); + goto ask; + } else if (ans_mtd[0]) { + int i,d,err; + for(err=0,i=0;!err && ans_mtd[i];i++) { + if (isdigit(ans_mtd[i])) + d++; /* count trailing digits */ + else if (isalpha(ans_mtd[i])) + d = 0; /* set trailing digits to 0 */ + else + err = 1; /* not alpha-numeric */ + } + if (err || i<3 || d!=2) { + printf("! Invalid microtitre dish name.\n"); + printf(" A microtitre dish must be a letter followed by two digits,\n with a possible alphanumeric prefix. Eg a87\n"); + goto ask; + } + } else { + strcpy(ans_mtd,def_mtd); + } + +} + + + + +ask_cosmid() +{ + ask: + + printf ("Cosmid [%s] ? ",def_cosmid); + gets(ans_cosmid); + + if ( strcmp(ans_cosmid,"?") == 0 ) { + printf("* Enter the name of the cosmid from which the subclones was made.\n"); + printf(" NO check is made on the correctness of the value entered.\n"); + goto ask; + } else if ( ans_cosmid[0] ){ + int i; + for (i=0; ipw_name); + shell_call("hostname",output, sizeof(output)); + fprintf(f," %s",output); + shell_call("date",output, sizeof(output)); + fprintf(f," %s",output); +#endif + + fprintf(f,"\n"); + fclose(f); + return 0; +} + + + + +main () +{ + + + printf("Maintain subclone database\n\n"); + + if ( set_defaults() ) { + fprintf (stderr,"Cannot open file '%s' ($SUBCLONES)\n",SUBCLONES); + exit(1); + } + + printf("Database=%s\n\n",SUBCLONES); + + ask_mtd(); + ask_cosmid(); + ask_method(); + ask_vector(); + ask_size(); + ask_comments(); + + ask_verify(); + + if ( strchr("Yy",ans_verify[0]) != NULL) { + printf("Updating file %s with new values...",SUBCLONES); + update(); + printf("done\n"); + } else { + printf("No update made\n"); + } + + +} diff --git a/src/vepe/eflt.h b/src/vepe/eflt.h new file mode 100644 index 0000000..40234ec --- /dev/null +++ b/src/vepe/eflt.h @@ -0,0 +1,36 @@ +/* +** Line types for experiment file +*/ +static char feature_ids[MAXIMUM_EFLTS][MAXIMUM_EFLT_LENGTH+1] = { + "CF", /* 0 cloning vector sequence file */ + "CN", /* 1 clone name */ + "CS", /* 2 cloning vector sequence present in sequence */ + "CV", /* 3 cloning vector type */ + "DR", /* 4 direction of read */ + "DT", /* 5 date of experiment */ + "EN", /* 6 experiment name */ + "EX", /* 7 experimental notes */ + "FM", /* 8 sequencing vector fragmentation method */ + "LN", /* 9 local format trace file name */ + "LT", /* 10 local format trace file type */ + "MC", /* 11 machine on which experiment ran */ + "MN", /* 12 machine generated trace file name */ + "MT", /* 13 machine generated trace file type */ + "OP", /* 14 operator */ + "PN", /* 15 primer name */ + "QR", /* 16 poor quality sequence present at right (3') end */ + "SC", /* 17 sequencing vector cloning site */ + "SF", /* 18 sequencing vector sequence file */ + "SI", /* 19 sequencing vector insertion length */ + "SL", /* 20 sequencing vector present at left (5') end */ + "SP", /* 21 sequencing vector primer site (relative to cloning site) */ + "SQ", /* 22 sequence */ + "SR", /* 23 sequencing vector present at right (3') end */ + "ST", /* 24 strands */ + "SV", /* 25 sequencing vector type */ + "TN", /* 26 template name */ + "QL", /* 27 poor quality sequence present at left (5') end */ + "PS", /* 28 processing status */ + "CC", /* 29 comments */ + "SS", /* 30 sequence to screen against */ +}; diff --git a/src/vepe/expFileIO.c b/src/vepe/expFileIO.c new file mode 100644 index 0000000..b4e59a3 --- /dev/null +++ b/src/vepe/expFileIO.c @@ -0,0 +1,552 @@ +/* + * expFileIO.c + * + * Routines for reading and writing to experiment files. + * + * 1. Opening experiment files + * 2. Reading information from an experiment file + * 3. Appending to experiment files + * 4. Closing an opened experiment file + * + */ + +#include +#include "expFileIO.h" +#include "eflt.h" + + +/* The only feature id that matters */ +#define SEQUENCE 22 + + + + + +static int exp_get_feature_index(char *e) +{ + int i; + + for (i = 0; i < MAXIMUM_EFLTS; i++) + if(strcmp(feature_ids[i],e)==0) return i; + + return -1; +} + + +static Exp_info *exp_create_info() +/* + * Allocate space for new experiment file information + */ +{ + Exp_info *new; + int i; + + new = (Exp_info *) malloc(sizeof(Exp_info)); + if (new != NULL) { + for(i=0; i< MAXIMUM_EFLTS ; i++) new->entry[i] = NULL; + } + new->fp = NULL; + + return new; +} + + +void exp_destroy_info(Exp_info *e) +/* + * Destroy experiment file information + */ +{ + int i; + if (e != NULL_Exp_info) { + for (i = 0; i < MAXIMUM_EFLTS; i++) + if (e->entry[i] != NULL) free(e->entry[i]); + if (e->fp != NULL) fclose(e->fp); + free(e); + } +} + + + + + + +static char *exp_read_sequence(FILE *fp) +/* + * Read from file a sequence, discarding all white space til a // is encountered + */ +{ + char *seq; + int seql; + char line[EXP_FILE_LINE_LENGTH+1]; + char *l; + + seql = 0; + seq = (char *)malloc(seql+1); + seq[0] = '\0'; + + l = fgets(line,EXP_FILE_LINE_LENGTH,fp); + while (l!= NULL && strncmp(l,"//",2)) { + char *a, *b; + for(a=b=line;*a;a++) + if (! isspace(*a)) *b++=*a; + *b = '\0'; + seql = seql + b-line; + seq = (char *)realloc(seq,seql+1); + strcat(seq,line); + l = fgets(line,EXP_FILE_LINE_LENGTH,fp); + } + + return seq; +} + + + + + + +Exp_info *exp_read_info(char *file) +/* + * Read in an experiment file and return handle + */ +{ + Exp_info *e; + char line[EXP_FILE_LINE_LENGTH+1]; + + e = exp_create_info(); + + /* + * open for read + */ + if ((e->fp = fopen(file,"r"))==NULL) { + exp_destroy_info(e); + return NULL_Exp_info; + } + + + if (e != NULL_Exp_info) { + while (fgets(line,EXP_FILE_LINE_LENGTH,e->fp) != NULL) { + char *c; + int entry; + /* + * zero terminate first argument + * set c to point to second argument + */ + for (c=line;*c && !isspace(*c); c++) ; + if (*c) { + *c++ = '\0'; + for (;*c && isspace(*c); c++) ; + } + + + entry = exp_get_feature_index(line); + if (entry >= 0) { + /* junk previous entry for same line type is there was one*/ + if (e->entry[entry]) free(e->entry[entry]); + + if (entry == SEQUENCE) { + e->entry[entry] = exp_read_sequence(e->fp); + } else { + int l; + l = strlen(c); + if (!l) l = 1; /* handle zero length lines elegantly */ + e->entry[entry] = (char *)malloc(l); + strncpy(e->entry[entry],c,l-1); + e->entry[entry][l-1] = '\0'; + } + } + } + } + + fclose(e->fp); + /* + * reopen for appending + */ + e->fp = fopen(file,"a"); + + return e; + +} + + +static int exp_check_eid_read(Exp_info *e,int id) +/* + * Check these are a valid combination and that + * an entry exists for read + */ +{ + return (e == NULL || id < 0 || id >= MAXIMUM_EFLTS || e->entry[id] == NULL || feature_ids[id][0]=='\0'); +} + +static int exp_check_eid_write(Exp_info *e,int id) +/* + * Check these are a valid combination and that + * an entry exists for write + */ +{ + return (e == NULL || id < 0 || id >= MAXIMUM_EFLTS || e->fp == NULL || feature_ids[id][0]=='\0'); +} + + + + + + +int exp_get_int(Exp_info *e, int id, int *val) +/* + * Get the integer for entry id + * returns: + * 0 - success + * 1 - no entry + */ +{ + if ( exp_check_eid_read(e,id) ) return 1; + *val = atoi(e->entry[id]); + return 0; +} + + +int exp_get_rng(Exp_info *e, int id, int *from, int *to) +/* + * Get the integer pair for entry id + * returns: + * 0 - success + * 1 - no entry + */ +{ + if ( exp_check_eid_read(e,id) ) return 1; + sscanf(e->entry[id],"%d..%d",from,to); + return 0; +} + + + +int exp_get_str(Exp_info *e, int id, char *s, int_fl s_l) +/* + * Get the string for entry id + * returns: + * 0 - success + * 1 - no entry + */ +{ + if ( exp_check_eid_read(e,id) ) return 1; + strncpy(s,e->entry[id],s_l); + + return 0; +} + + +static int exp_append_str(Exp_info *e, int id, char *s) +/* + * Append the string to experiment file for entry id + * returns: + * 0 - success + * 1 - no update + */ +{ + char *copy; + if ( (copy = (char *)malloc(strlen(s)+1))==NULL ) return 1; + if (fprintf(e->fp,"%s %s\n",feature_ids[id],s)<0) return 1; + /* copied and written successfully... now switch */ + strcpy(copy,s); + free(e->entry[id]); + e->entry[id] = copy; + return 0; +} + + +int exp_put_int(Exp_info *e, int id, int *val) +/* + * Append the integer for entry id to the experiment file + * returns: + * 0 - success + * 1 - no update + */ +{ + char buf[EXP_FILE_LINE_LENGTH]; + if ( exp_check_eid_write(e,id) ) return 1; + sprintf(buf,"%d",*val); + return exp_append_str(e,id,buf); +} + + +int exp_put_rng(Exp_info *e, int id, int *from, int *to) +/* + * Append the integer pair for entry id to the experiment file + * returns: + * 0 - success + * 1 - no update + */ +{ + char buf[EXP_FILE_LINE_LENGTH]; + if ( exp_check_eid_write(e,id) ) return 1; + sprintf(buf,"%d..%d",*from,*to); + return exp_append_str(e,id,buf); +} + + + +int exp_put_str(Exp_info *e, int id, char *s, int_fl s_l) +/* + * Append the string for entry id to the experiment file + * returns: + * 0 - success + * 1 - no update + */ +{ + if ( exp_check_eid_write(e,id) ) return 1; + /* don't allow multi-line entries to be written */ + if ( id == SEQUENCE ) return 1; + return exp_append_str(e,id,s); +} + + +/* + * FORTRAN INTERFACE + */ + +static void c2fstr(char *c, int max_c, char *f, int max_f) +{ +#define min(A,B) ( (A) < (B) ? (A) : (B) ) + int i; + i = strlen(c); + i = min(i,max_f); + i = min(i,max_c); + strncpy(f,c,i); + for( ; i 0 && (isspace(f[max_f-1]) || f[max_f-1]=='\0'); max_f--); + return max_f; +} + +static void f2cstr(char *f, int max_f, char *c, int max_c) +{ + int i; + + i = min(fstrlen(f,max_f),max_c); + strncpy(c,f,i); + c[i]='\0'; +} + + + + + +/************************************************************* + * FORTRAN INTERFACE + *************************************************************/ + + + +static int init_done = 0; +static int NHandles = 0; +static Exp_info **Handles = NULL; + +static int initialise() +{ + int i; + + if (init_done) return 0; + init_done++; + + NHandles = getdtablesize(); + if (!NHandles) return 1; + + if ( (Handles = (Exp_info **)malloc(sizeof(Exp_info *) * NHandles)) == NULL) { + NHandles = 0; + return 1; + } + + for (i=0; i NHandles); +} + + + +int_f expopn_(char *fn, int_fl fn_l) +/* + * FORTRAN interface to exp_open_file() + */ +{ + char cfn[1025]; + int handle; + + if ( (handle = get_free_handle()) >= 0 ) { + f2cstr(fn,fn_l,cfn,1024); + Handles[handle] = exp_read_info(cfn); + } + + return (int_f) (handle+1); +} + + + +int_f expkil_(int_f *handle) +/* + * FORTRAN interface to exp_destroy_info + */ +{ + Exp_info *e; + if ( check_handle(handle) ) return 0; + e = (Exp_info *) Handles[(int)(*handle)-1]; + + exp_destroy_info(e); + + Handles[(int)(*handle)-1] = NULL; + *handle = 0; + + return 0; +} + +int_f expri_(int_f *handle, int_f *id, int_f *val) +/* + * FORTRAN interface to exp_get_int + */ +{ + Exp_info *e; + if ( check_handle(handle) ) return 1; + e = (Exp_info *) Handles[(int)(*handle)-1]; + + return exp_get_int(e, (int)*id, (int *)val); +} + + +int_f exprr_(int_f *handle, int_f *id, int_f *from, int_f *to) +/* + * FORTRAN interface to exp_get_rng + */ +{ + Exp_info *e; + if ( check_handle(handle) ) return 1; + e = (Exp_info *) Handles[(int)(*handle)-1]; + + return exp_get_rng(e,(int)*id,(int *)from,(int *)to); + +} + +int_f exprsa_(int_f *handle, int_f *id, char *s, int_f *max_len, int_fl s_l) +/* + * FORTRAN interface to exp_get_str workalike + * NOTE: for use with FORTRAN CHARACTER arrays instead CHARACTER strings + */ +{ + Exp_info *e; + if ( check_handle(handle) ) return 1; + e = (Exp_info *) Handles[(int)(*handle)-1]; + + if ( exp_check_eid_read(e,*id) ) return 1; + c2fstr(e->entry[*id],(int)*max_len,s,(int)*max_len); + return 0; +} + + +int_f exprs_(int_f *handle, int_f *id, char *s, int_fl s_l) +/* + * FORTRAN interface to exp_get_str workalike + * NOTE: for use with FORTRAN CHARACTER strings instead CHARACTER arrays + */ +{ + Exp_info *e; + if ( check_handle(handle) ) return 1; + e = (Exp_info *) Handles[(int)(*handle)-1]; + + if ( exp_check_eid_read(e,*id) ) return 1; + c2fstr(e->entry[*id],s_l,s,s_l); + return 0; +} + + +int_f expwi_(int_f *handle, int_f *id, int_f *val) +/* + * FORTRAN interface to exp_put_int + */ +{ + Exp_info *e; + if ( check_handle(handle) ) return 1; + e = (Exp_info *) Handles[(int)(*handle)-1]; + + return exp_put_int(e, (int)*id, (int *)val); +} + + +int_f expwr_(int_f *handle, int_f *id, int_f *from, int_f *to) +/* + * FORTRAN interface to exp_put_rng + */ +{ + Exp_info *e; + if ( check_handle(handle) ) return 1; + e = (Exp_info *) Handles[(int)(*handle)-1]; + + return exp_put_rng(e, (int)*id, (int *)from, (int *)to); +} + + +int_f expwsa_(int_f *handle, int_f *id, char *s, int_f *max_len, int_fl s_l) +/* + * FORTRAN interface to exp_put_str workalike + * NOTE: for use with FORTRAN CHARACTER arrays instead CHARACTER strings + */ +{ + Exp_info *e; + char buf[EXP_FILE_LINE_LENGTH]; + if ( check_handle(handle) ) return 1; + e = (Exp_info *) Handles[(int)(*handle)-1]; + + + if ( exp_check_eid_write(e,*id) ) return 1; + /* don't allow multi-line entries to be written */ + if (*id == SEQUENCE ) return 1; + f2cstr(s,(int)*max_len,buf,sizeof(buf)); + return exp_append_str(e,*id,buf); + +} + +int_f expws_(int_f *handle, int_f *id, char *s, int_fl s_l) +/* + * FORTRAN interface to exp_put_str workalike + * NOTE: for use with FORTRAN CHARACTER strings instead CHARACTER arrays + */ +{ + char buf[EXP_FILE_LINE_LENGTH]; + Exp_info *e; + if ( check_handle(handle) ) return 1; + e = (Exp_info *) Handles[(int)(*handle)-1]; + + + if ( exp_check_eid_write(e,*id) ) return 1; + /* don't allow multi-line entries to be written */ + if (*id == SEQUENCE ) return 1; + f2cstr(s,s_l,buf,sizeof(buf)); + return exp_append_str(e,*id,buf); +} + + diff --git a/src/vepe/expFileIO.h b/src/vepe/expFileIO.h new file mode 100644 index 0000000..a438968 --- /dev/null +++ b/src/vepe/expFileIO.h @@ -0,0 +1,173 @@ +#ifndef _read_exp_h +#define _read_exp_h + +#include "fort.h" + +/* +** Definitions +*/ +#define MAXIMUM_EFLT_LENGTH 4 +#define MAXIMUM_EFLTS 32 +#define EFLT_FILE_LINE_LENGTH 128 +#define EXP_FILE_LINE_LENGTH 128 + +typedef struct { + char *entry[MAXIMUM_EFLTS]; + FILE *fp; +} Exp_info; + +#define NULL_Exp_info ( (Exp_info *) NULL ) + + + + + + /*************************************************************************************/ + + +extern void exp_destroy_info(Exp_info *e); +/* +** Destroy experiment file information +*/ + + + + + + + + + + +extern Exp_info *exp_read_info(char *file); +/* +** Read in an experiment file and return handle +*/ + + + + +extern int exp_get_int(Exp_info *e, int id, int *val); +/* +** Get the integer for entry id +** returns: +** 0 - success +** 1 - no entry +*/ + + +extern int exp_get_rng(Exp_info *e, int id, int *from, int *to); +/* +** Get the integer pair for entry id +** returns: +** 0 - success +** 1 - no entry +*/ + + +extern int exp_get_str(Exp_info *e, int id, char *s, int_fl s_l); +/* +** Get the string for entry id +** returns: +** 0 - success +** 1 - no entry +*/ + + +extern int exp_put_int(Exp_info *e, int id, int *val); +/* +** Append the integer for entry id to the experiment file +** returns: +** 0 - success +** 1 - no update +*/ + + +extern int exp_put_rng(Exp_info *e, int id, int *from, int *to); +/* +** Append the integer pair for entry id to the experiment file +** returns: +** 0 - success +** 1 - no update +*/ + + + +extern int exp_put_str(Exp_info *e, int id, char *s, int_fl s_l); +/* +** Append the string for entry id to the experiment file +** returns: +** 0 - success +** 1 - no update +*/ + + +/* +** FORTRAN INTERFACE +*/ + + + +extern int_f expopn_(char *fn, int_fl fn_l); +/* +** FORTRAN interface to exp_open_file() +*/ + +extern int_f expkil_(int_f *le); +/* +** FORTRAN interface to exp_destroy_info +*/ + +extern int_f expri_(int_f *le, int_f *id, int_f *val); +/* +** FORTRAN interface to exp_get_int +*/ + + +extern int_f exprr_(int_f *le, int_f *id, int_f *from, int_f *to); +/* +** FORTRAN interface to exp_get_rng +*/ + + +extern int_f exprsa_(int_f *le, int_f *id, char *s, int_f *max_len, int_fl s_l); +/* +** FORTRAN interface to exp_get_str workalike +** NOTE: for use with FORTRAN CHARACTER arrays instead CHARACTER strings +*/ + +extern int_f exprs_(int_f *le, int_f *id, char *s, int_fl s_l); +/* +** FORTRAN interface to exp_get_str workalike +** NOTE: for use with FORTRAN CHARACTER strings instead CHARACTER arrays +*/ + +extern int_f expwi_(int_f *le, int_f *id, int_f *val); +/* +** FORTRAN interface to exp_put_int +*/ + + +extern int_f expwr_(int_f *le, int_f *id, int_f *from, int_f *to); +/* +** FORTRAN interface to exp_put_rng +*/ + + + +extern int_f expwsa_(int_f *le, int_f *id, char *s, int_f *max_len, int_fl s_l); +/* +** FORTRAN interface to exp_put_str workalike +** NOTE: for use with FORTRAN CHARACTER arrays instead CHARACTER strings +*/ + + + +extern int_f expws_(int_f *le, int_f *id, char *s, int_fl s_l); +/* +** FORTRAN interface to exp_put_str workalike +** NOTE: for use with FORTRAN CHARACTER strings instead CHARACTER arrays +*/ + + +#endif /* _read_exp_h */ diff --git a/src/vepe/expio.f b/src/vepe/expio.f new file mode 100644 index 0000000..409b6bf --- /dev/null +++ b/src/vepe/expio.f @@ -0,0 +1,135 @@ +C routines to read and write data to the experiment files +C rexgel read gel +C rexcvn read cloning vector name +C rexsvn read sequencing vector name +C rexsvc read cloning site +C rexsvp read primer site +C rexpdp read poor data positions +C rexsvp read sequencing vector positions in reading +C wexsvp write sequencing vector positions +C wexcvp write cloning vector positions +C +C +C + INTEGER FUNCTION REXGEL(IDEV,GEL,LENGTH) + CHARACTER GEL(LENGTH) + INTEGER EXPRSA + EXTERNAL EXPRSA +C rexgel read gel + JOB = 22 + REXGEL = EXPRSA(IDEV,JOB,GEL,LENGTH) + JOB = LENGTH + DO 10 I=1,JOB + IF(GEL(I).EQ.' ') THEN + LENGTH = I-1 + RETURN + END IF + 10 CONTINUE + END + INTEGER FUNCTION REXCVN(IDEV,NAME) + CHARACTER NAME*(*) + INTEGER EXPRS + EXTERNAL EXPRS +C rexcvn read cloning vector name + JOB = 3 + REXCVN = EXPRS(IDEV,JOB,NAME) + END + INTEGER FUNCTION REXCVF(IDEV,NAME) + CHARACTER NAME*(*) + INTEGER EXPRS + EXTERNAL EXPRS +C rexcvf read cloning vector file name + JOB = 0 + REXCVF = EXPRS(IDEV,JOB,NAME) + END + INTEGER FUNCTION REXSVF(IDEV,NAME) + CHARACTER NAME*(*) + INTEGER EXPRS + EXTERNAL EXPRS +C rexsvf read sequencing vector file name + JOB = 18 + REXSVF = EXPRS(IDEV,JOB,NAME) + END + INTEGER FUNCTION REXSVN(IDEV,NAME) + CHARACTER NAME*(*) + INTEGER EXPRS + EXTERNAL EXPRS +C rexsvn read sequencing vector name + JOB = 25 + REXSVN = EXPRS(IDEV,JOB,NAME) + END + INTEGER FUNCTION REXSVC(IDEV,ICSITE) + INTEGER EXPRI + EXTERNAL EXPRI +C rexsvc read cloning site + JOB = 17 + REXSVC = EXPRI(IDEV,JOB,ICSITE) + IF(REXSVC.NE.0) ICSITE = 0 + END + INTEGER FUNCTION REXSVQ(IDEV,IPSITE) + INTEGER EXPRI + EXTERNAL EXPRI +C rexsvq read primer site + JOB = 21 + REXSVQ = EXPRI(IDEV,JOB,IPSITE) + IF(REXSVQ.NE.0) IPSITE = 0 + END + INTEGER FUNCTION REXPDP(IDEV,LEFT,IRIGHT) + INTEGER EXPRI + EXTERNAL EXPRI +C rexpdp read poor data positions + LEFT = 0 + JOB = 16 + REXPDP = EXPRI(IDEV,JOB,IRIGHT) + IF(REXPDP.NE.0) THEN + IRIGHT = 0 + END IF + JOB = 27 + REXPDP = EXPRI(IDEV,JOB,LEFT) + IF(REXPDP.NE.0) THEN + LEFT = 0 + END IF + END + INTEGER FUNCTION REXSVP(IDEV,LEFT,IRIGHT) + INTEGER EXPRI + EXTERNAL EXPRI +C rexsvp read sequencing vector positions + JOB = 20 + REXSVP = EXPRI(IDEV,JOB,LEFT) + IF(REXSVP.NE.0) THEN + LEFT = 0 + END IF + JOB = 23 + REXSVP = EXPRI(IDEV,JOB,IRIGHT) + IF(REXSVP.NE.0) THEN + IRIGHT = 0 + END IF + END + INTEGER FUNCTION REXCVP(IDEV,LEFT,IRIGHT) + INTEGER EXPRR + EXTERNAL EXPRR +C rexcvp read cloning vector positions + JOB = 2 + REXCVP = EXPRR(IDEV,JOB,LEFT,IRIGHT) + IF(REXCVP.NE.0) THEN + LEFT = 0 + IRIGHT = 0 + END IF + END + INTEGER FUNCTION WEXCVP(IDEV,LEFT,IRIGHT) + INTEGER EXPWR + EXTERNAL EXPWR +C rexpst write cloning vector positions + JOB = 2 + WEXCVP = EXPWR(IDEV,JOB,LEFT,IRIGHT) + END + INTEGER FUNCTION WEXSVP(IDEV,LEFT,IRIGHT) + INTEGER EXPWI + EXTERNAL EXPWI +C rexpst write sequencing vector positions + JOB = 20 + WEXSVP = EXPWI(IDEV,JOB,LEFT) + IF(WEXSVP.NE.0) RETURN + JOB = 23 + WEXSVP = EXPWI(IDEV,JOB,IRIGHT) + END diff --git a/src/vepe/makefile-alpha b/src/vepe/makefile-alpha new file mode 100644 index 0000000..ff9fb35 --- /dev/null +++ b/src/vepe/makefile-alpha @@ -0,0 +1,136 @@ +# Makefile for vepe - Alpha version +# ------------------------------- + +PROGS= vepe + +all: $(PROGS) + + + + + +# +# ``Interesting'' suffices that make needs to know about +# +.SUFFIXES: .a $(SUFFIXES) + + +# +# Directories +# +SRC = . +#STADENSRC=/nfs/rutland/home3/pubseq/inhouse/share/src/staden +STADENSRC=$(SRC)/../staden +STADENBIN=$(STADENSRC)/alpha-binaries +# Use the ted sources compiled for staden executables +INCLUDES= -I$(STADENSRC) + + +# +# Compilers and flags +# +# debug on +CC= cc +CFLAGS= -g -std1 -ieee_with_inexact $(INCLUDES) +F77= f77 +FFLAGS= -g +LD= f77 +LDFLAGS= -g -non_shared + +FLIBS= -lfor -lutil -lUfor -lots -lFutil -lm -lfor -lutil -lUfor -lots -lFutil +XLIBS= -lXaw -lXt -lXmu -lXext -lX11 +#LFLAGS= $(FLIBS) +XLFLAGS= $(FLIBS) $(XLIBS) + +# +# Avoid compilation of objects in remote directories +# +$(STADENBIN)/%.o : $(STADENSRC)/%.f +$(STADENBIN)/%.o : $(STADENSRC)/%.c + +# +# Common objects, needed by every program +# +COMMONOBJS=\ + $(STADENBIN)/seeme-dec.o\ + $(STADENBIN)/nxspec.o\ + $(STADENBIN)/userface.o\ + $(STADENBIN)/userfacecom.o\ + $(STADENBIN)/helpnmenu.o\ + $(STADENBIN)/nxhelpmenu.o\ + $(STADENBIN)/FtoC.o\ + $(STADENBIN)/subs89.a\ + + +# +# Building the programs +# This should be just a linking phase because all of the object +# files and library files are generated using implicit rules. +# We use the fortran compiler to do linking. +# + +# +# Vector excision program +# + +VEP=\ + vep.o\ + $(STADENBIN)/dias89.a\ + $(STADENBIN)/subs89.a + + +VEPOBJS=\ + $(VEP)\ + $(COMMONOBJS) + + +vep: $(VEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPOBJS) $(LFLAGS) + + + +VEPE=\ + vepe.o\ + $(STADENBIN)/dias89.a\ + expio.o\ + expFileIO.o + + +VEPEOBJS=\ + $(VEPE)\ + $(COMMONOBJS) + + +vepe: $(VEPEOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPEOBJS) $(LFLAGS) + +COBJS = expFileIO.o testc.o +FOBJS = expFileIO.o testf.o + + +testc: $(COBJS) + $(CC) $(CFLAGS) -o $@ $(COBJS) + +testf: $(FOBJS) + $(F77) $(FFLAGS) -o $@ $(FOBJS) + + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o: %.c + $(CC) $(CFLAGS) -c $< +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + +# +# Files requiring simple FORTRAN compilation (thing.f -> thing.o) +# +%.o: $(SRC)/%.f + $(F77) $(FFLAGS) -c $< + +clean: + rm -f $(PROGS) *.o *.a *~ + + +install: clean all diff --git a/src/vepe/makefile-dec b/src/vepe/makefile-dec new file mode 100644 index 0000000..a55a27f --- /dev/null +++ b/src/vepe/makefile-dec @@ -0,0 +1,139 @@ +# Makefile for vepe - dec ultrix version +# -------------------------------------- + +PROGS= vepe + +all: $(PROGS) + + + + + +# +# ``Interesting'' suffices that make needs to know about +# +.SUFFIXES: .a $(SUFFIXES) + + +# +# Directories +# +SRC=. +#STADENSRC=/nfs/rutland/home3/pubseq/inhouse/share/src/staden +STADENSRC=$(SRC)/../staden +STADENBIN=$(STADENSRC) +MISCSRC=$(SRC)/../Misc +MISCBIN=$(MISCSRC) +# Use the ted sources compiled for staden executables +INCLUDES= -I$(STADENSRC) -I$(MISCSRC) + + +# +# Compilers and flags +# +# debug on +CC= c89 +CFLAGS= -g -common $(INCLUDES) +F77= f77 +FFLAGS= -g +LD= f77 +LDFLAGS= -g + +FLIBS= +XLIBS= -lXaw -lXt -lXmu -lXext -lX11 +#LFLAGS= $(FLIBS) +XLFLAGS= $(FLIBS) $(XLIBS) + +# +# Avoid compilation of objects in remote directories +# +$(STADENBIN)/%.o : $(STADENSRC)/%.f +$(STADENBIN)/%.o : $(STADENSRC)/%.c + +# +# Common objects, needed by every program +# +COMMONOBJS=\ + $(STADENBIN)/seeme-dec.o\ + $(STADENBIN)/nxspec.o\ + $(STADENBIN)/userface.o\ + $(STADENBIN)/userfacecom.o\ + $(STADENBIN)/helpnmenu.o\ + $(STADENBIN)/nxhelpmenu.o\ + $(STADENBIN)/FtoC.o\ + $(STADENBIN)/subs89.a\ + $(MISCBIN)/misc.a + + +# +# Building the programs +# This should be just a linking phase because all of the object +# files and library files are generated using implicit rules. +# We use the fortran compiler to do linking. +# + +# +# Vector excision program +# + +VEP=\ + vep.o\ + $(STADENBIN)/dias89.a\ + $(STADENBIN)/subs89.a + + +VEPOBJS=\ + $(VEP)\ + $(COMMONOBJS) + + +vep: $(VEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPOBJS) $(LFLAGS) + + + +VEPE=\ + vepe.o\ + $(STADENBIN)/dias89.a\ + expio.o\ + expFileIO.o + + +VEPEOBJS=\ + $(VEPE)\ + $(COMMONOBJS) + + +vepe: $(VEPEOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPEOBJS) $(LFLAGS) + +COBJS = expFileIO.o testc.o +FOBJS = expFileIO.o testf.o + + +testc: $(COBJS) + $(CC) $(CFLAGS) -o $@ $(COBJS) + +testf: $(FOBJS) + $(F77) $(FFLAGS) -o $@ $(FOBJS) + + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o: %.c + $(CC) $(CFLAGS) -c $< +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + +# +# Files requiring simple FORTRAN compilation (thing.f -> thing.o) +# +%.o: $(SRC)/%.f + $(F77) $(FFLAGS) -c $< + +clean: + rm -f $(PROGS) *.o *.a *~ + + +install: clean all diff --git a/src/vepe/makefile-sgi b/src/vepe/makefile-sgi new file mode 100644 index 0000000..63ae8b6 --- /dev/null +++ b/src/vepe/makefile-sgi @@ -0,0 +1,146 @@ +# Makefile for vepe - SGI version +# ------------------------------- + +PROGS= vepe + +SHELL=/bin/sh + + + + +all: $(PROGS) + + + + + +# +# ``Interesting'' suffices that make needs to know about +# +.SUFFIXES: .a $(SUFFIXES) + + +# +# Directories +# +SRC=. +STADENSRC=$(SRC)/../staden +STADENBIN=$(STADENSRC) +# Use the ted sources compiled for staden executables +INCLUDES= -I$(STADENSRC) + + +# +# Compilers and flags +# +# debug on +CC= cc +CFLAGS= -g -ansi -prototypes $(INCLUDES) +F77= f77 +FFLAGS= -g +LD= cc +LDFLAGS= -g + +FLIBS= -lF77 -lU77 -lm -lI77 -lisam +XLIBS= -lXaw -lXt -lXmu -lXext -lX11 +LFLAGS= $(FLIBS) +XLFLAGS= $(FLIBS) $(XLIBS) + +# +# Avoid compilation of objects in remote directories +# +$(STADENBIN)/%.o : $(STADENSRC)/%.f +$(STADENBIN)/%.o : $(STADENSRC)/%.c + +# +# Common objects, needed by every program +# +COMMONOBJS=\ + $(STADENBIN)/seeme-sg.o\ + $(STADENBIN)/nxspec.o\ + $(STADENBIN)/userface.o\ + $(STADENBIN)/userfacecom.o\ + $(STADENBIN)/helpnmenu.o\ + $(STADENBIN)/nxhelpmenu.o\ + $(STADENBIN)/FtoC.o\ + $(STADENBIN)/subs89.a + + +# +# Building the programs +# This should be just a linking phase because all of the object +# files and library files are generated using implicit rules. +# We use the fortran compiler to do linking. +# + +# +# Vector excision program +# + +VEP=\ + vep.o\ + $(STADENBIN)/dias89.a\ + $(STADENBIN)/subs89.a + + +VEPOBJS=\ + $(VEP)\ + $(COMMONOBJS) + + +vep: $(VEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPOBJS) $(LFLAGS) + + + +VEPE=\ + vepe.o\ + $(STADENBIN)/dias89.a\ + expio.o\ + expFileIO.o + + +VEPEOBJS=\ + $(VEPE)\ + $(COMMONOBJS) + + +vepe: $(VEPEOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPEOBJS) $(LFLAGS) + +COBJS = expFileIO.o testc.o +FOBJS = expFileIO.o testf.o + + +testc: $(COBJS) + $(CC) $(CFLAGS) -o $@ $(COBJS) + +testf: $(FOBJS) + $(F77) $(FFLAGS) -o $@ $(FOBJS) + +# +# Files requiring simple FORTRAN compilation (thing.f -> thing.o) +# +%.o: $(SRC)/%.f + $(F77) $(FFLAGS) -c $< + + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o: %.c + $(CC) $(CFLAGS) -c $< +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + + + + + + + +clean: + rm -f $(PROGS) *.o *.a *~ + + +install: clean all diff --git a/src/vepe/makefile-solaris b/src/vepe/makefile-solaris new file mode 100644 index 0000000..65fe955 --- /dev/null +++ b/src/vepe/makefile-solaris @@ -0,0 +1,154 @@ +# Makefile for vepe - Solaris (sparc) version +# ------------------------------------------- + +PROGS= vepe + + + + +all: $(PROGS) + + + + + +# +# ``Interesting'' suffices that make needs to know about +# +.SUFFIXES: .a $(SUFFIXES) + + +# +# Directories +# +SRC=. +#STADENSRC=/nfs/rutland/home3/pubseq/inhouse/share/src/staden +STADENSRC=$(SRC)/../staden +STADENBIN=$(STADENSRC) +# Use the ted sources compiled for staden executables +INCLUDES= -I$(STADENSRC) + + +# +# Compilers and flags +# +# debug on +CC= gcc +GCCWARNINGS = -Wreturn-type -Wunused -Wswitch -Wcomment -W -Wformat \ + -Wuninitialized +CFLAGS= -g -ansi -pedantic $(GCCWARNINGS) $(INCLUDES) +# KFLAGS - for compiling non ANSI C modules +KFLAGS= -g $(INCLUDES) +F77= f77 +FFLAGS= -ansi -g -C -Nx500 + +LD = f77 +SPRO = /opt/SUNWspro/SC2.0.1 +LDFLAGS = -Bstatic -g -L$(SPRO) -L/usr/ccs/lib -L/usr/ucblib -L/usr/openwin/lib +LIBS = -lm -lucb -lelf -Bdynamic -ldl -Bstatic -lsocket -lnsl -lintl +FLIBS = -lV77 -lF77 -lM77 +XLIBS = -lXaw -lXt -lXmu -lXext -lX11 +LFLAGS = $(FLIBS) $(LIBS) +XLFLAGS = $(FLIBS) $(XLIBS) $(LIBS) + +# For stdargs on the Sun +#GNULIB = /usr/local/lib/gcc-gnulib + +# +# Avoid compilation of objects in remote directories +# +$(STADENBIN)/%.o : $(STADENSRC)/%.f +$(STADENBIN)/%.o : $(STADENSRC)/%.c + +# +# Common objects, needed by every program +# +COMMONOBJS=\ + $(STADENBIN)/seeme.o\ + $(STADENBIN)/nxspec.o\ + $(STADENBIN)/userface.o\ + $(STADENBIN)/userfacecom.o\ + $(STADENBIN)/helpnmenu.o\ + $(STADENBIN)/nxhelpmenu.o\ + $(STADENBIN)/FtoC.o\ + $(STADENBIN)/subs89.a + + +# +# Building the programs +# This should be just a linking phase because all of the object +# files and library files are generated using implicit rules. +# We use the fortran compiler to do linking. +# + +# +# Vector excision program +# + +VEP=\ + vep.o\ + $(STADENBIN)/dias89.a\ + $(STADENBIN)/subs89.a + + +VEPOBJS=\ + $(VEP)\ + $(COMMONOBJS) + + +vep: $(VEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPOBJS) $(LFLAGS) + + + +VEPE=\ + vepe.o\ + $(STADENBIN)/dias89.a\ + expio.o\ + expFileIO.o + + +VEPEOBJS=\ + $(VEPE)\ + $(COMMONOBJS) + + +vepe: $(VEPEOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPEOBJS) $(LFLAGS) + +COBJS = expFileIO.o testc.o +FOBJS = expFileIO.o testf.o + + +testc: $(COBJS) + $(CC) $(CFLAGS) -o $@ $(COBJS) + +testf: $(FOBJS) + $(F77) $(FFLAGS) -o $@ $(FOBJS) + +# +# Files requiring simple FORTRAN compilation (thing.f -> thing.o) +# +%.o: $(SRC)/%.f + $(F77) $(FFLAGS) -c $< + + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o: %.c + $(CC) $(CFLAGS) -c $< +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + + + + + + + +clean: + rm -f $(PROGS) *.o *.a *~ + + +install: clean all diff --git a/src/vepe/makefile-sun b/src/vepe/makefile-sun new file mode 100644 index 0000000..4ea0a53 --- /dev/null +++ b/src/vepe/makefile-sun @@ -0,0 +1,153 @@ +# Makefile for vepe - SunOS (sparc) version +# ---------------------------------------- + +PROGS= vepe + + + + +all: $(PROGS) + + + + + +# +# ``Interesting'' suffices that make needs to know about +# +.SUFFIXES: .a $(SUFFIXES) + + +# +# Directories +# +SRC=. +#STADENSRC=/nfs/rutland/home3/pubseq/inhouse/share/src/staden +STADENSRC=$(SRC)/../staden +STADENBIN=$(STADENSRC) +# Use the ted sources compiled for staden executables +INCLUDES= -I$(STADENSRC) + + +# +# Compilers and flags +# +# debug on +CC= gcc +GCCWARNINGS = -Wreturn-type -Wunused -Wswitch -Wcomment -W -Wformat \ + -Wuninitialized +CFLAGS= -g -ansi -pedantic $(GCCWARNINGS) $(INCLUDES) +# KFLAGS - for compiling non ANSI C modules +KFLAGS= -g $(INCLUDES) +F77= f77 +#FFLAGS= -ansi -g -C -Nx500 +FFLAGS= -ansi -O -Nx500 +LD= gcc +LDFLAGS= -g + +FLIBS= -lF77 -lI77 -lU77 -lm +XLIBS= -lXaw -lXt -lXmu -lXext -lX11 +LFLAGS= $(FLIBS) +XLFLAGS= $(FLIBS) $(XLIBS) + +# For stdargs on the Sun +#GNULIB = /usr/local/lib/gcc-gnulib + +# +# Avoid compilation of objects in remote directories +# +$(STADENBIN)/%.o : $(STADENSRC)/%.f +$(STADENBIN)/%.o : $(STADENSRC)/%.c + +# +# Common objects, needed by every program +# +COMMONOBJS=\ + $(STADENBIN)/seeme.o\ + $(STADENBIN)/nxspec.o\ + $(STADENBIN)/userface.o\ + $(STADENBIN)/userfacecom.o\ + $(STADENBIN)/helpnmenu.o\ + $(STADENBIN)/nxhelpmenu.o\ + $(STADENBIN)/FtoC.o\ + $(STADENBIN)/subs89.a + + +# +# Building the programs +# This should be just a linking phase because all of the object +# files and library files are generated using implicit rules. +# We use the fortran compiler to do linking. +# + +# +# Vector excision program +# + +VEP=\ + vep.o\ + $(STADENBIN)/dias89.a\ + $(STADENBIN)/subs89.a + + +VEPOBJS=\ + $(VEP)\ + $(COMMONOBJS) + + +vep: $(VEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPOBJS) $(LFLAGS) + + + +VEPE=\ + vepe.o\ + $(STADENBIN)/dias89.a\ + expio.o\ + expFileIO.o + + +VEPEOBJS=\ + $(VEPE)\ + $(COMMONOBJS) + + +vepe: $(VEPEOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPEOBJS) $(LFLAGS) + +COBJS = expFileIO.o testc.o +FOBJS = expFileIO.o testf.o + + +testc: $(COBJS) + $(CC) $(CFLAGS) -o $@ $(COBJS) + +testf: $(FOBJS) + $(F77) $(FFLAGS) -o $@ $(FOBJS) + +# +# Files requiring simple FORTRAN compilation (thing.f -> thing.o) +# +%.o: $(SRC)/%.f + $(F77) $(FFLAGS) -c $< + + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o: %.c + $(CC) $(CFLAGS) -c $< +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + + + + + + + +clean: + rm -f $(PROGS) *.o *.a *~ + + +install: clean all diff --git a/src/vepe/vepe.f b/src/vepe/vepe.f new file mode 100644 index 0000000..3524365 --- /dev/null +++ b/src/vepe/vepe.f @@ -0,0 +1,761 @@ +C 18 Feb 1993 forced cloning vector clipped regions to be >MINVEC long +C 20 October 1992 +C IDEVE was being set to 0 before EXPKIL() and so the C files +C were never being closed. +C + PARAMETER (MAXSEQ = 50000, + + MAXWLN = 6, + + LCONST = 4*MAXWLN, + + MAXWRD = 4**MAXWLN, + + MAXDEV = 5, + + NAMLEN = 80) + CHARACTER SEQV(MAXSEQ*2),SEQG(MAXSEQ),SEQC(MAXSEQ) + INTEGER WORDP(MAXWRD),POSN(MAXSEQ) + REAL HIST(-MAXSEQ:MAXSEQ) + INTEGER CONSTS(0:LCONST),DEVNOS(MAXDEV) + INTEGER SEQVI(MAXSEQ),SEQGI(MAXSEQ),RC,POORR,POORL + CHARACTER*(NAMLEN) FILNAM,HELPF,NAME,FILNMV + PARAMETER (MAXPRM = 22) + CHARACTER PROMPT(2)*(MAXPRM) + INTEGER GNFFOF,REXGEL,REXSVF,REXSVC,REXSVP,REXPDP,REXCVF + INTEGER WEXSVP,WEXCVP,SVLCLP,SVRCLP,EXPOPN,REXSVQ + EXTERNAL GNFFOF,REXGEL,REXSVF,REXSVC,REXSVP,REXPDP,REXCVF + EXTERNAL WEXSVP,WEXCVP,EXPOPN,REXSVQ +C +C new experiment file version of vep (vepe) +C +C +C +C This routine prepares a reading for the assembly program. +C It compares the sequence against vectors and marks any found +C Vector clipping is of 4 types: +C 1) find 5' cloning site +C 2) look for 3' cloning site +C 3) test for insert being all vector +C 4) look for cosmid "vector" +C The first 3 can all be performed using one sequence, and are made easier +C by telling the program exactly where the cloning site is, and which of +C the 3 it is performing. Types 1,2 and 3 look only in one orientation, +C whereas 4 should check both strands. Additionally they +C differ in their outcomes: 1 and 2 write a new file with the clippoints +C marked, 3 scrubs the reading (does not add it to a file of file names), +C 4 writes out a new file if the reading contains some non vector sequence. +C The clip should be marked differently for cosmid vector, just so we know where +C it is (it can be tagged). +C Clipping off crap should probably be done first by looking at the traces. +C What do we need to tell the program? +C 1) name of vector file +C 2) position of cloning site +C 3) position of primer +C It would be helpful to have a standard orientation for vector sequences +C for example Cloning site------ ... ------etis gninolC for ? strand +C NOTE that vector file should not have <---name.0001----> at the start +C +C error values written to file of failed names +C +C 1 couldnt open expt file +C 2 couldnt get reading from expt file +C 3 reading too short +C 4 couldnt find vector filename in expt file +C 5 couldnt find cloning site in expt file +C 6 couldnt find primer site in expt file +C 7 couldnt open vector file +C 8 failed to write to expt file +C 9 completely vector +C + ICG = 0 + ICB = 0 + IPG = 0 + IDM = 5 + CALL INITLU(IDM) + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + IDEVNI = DEVNOS(1) + IDEVV = DEVNOS(2) + IDEVNO = DEVNOS(3) + IDEVNF = DEVNOS(5) + WRITE(KBOUT,*)'vepe v2.0: vector excising program. June 92' + PROMPT(1) = 'Mark sequencing vector' + PROMPT(2) = 'Mark cloning vector' + JOB = 1 + CALL RADION('Select task',PROMPT,2,JOB, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(JOB.LT.1) STOP + FILNAM = ' ' + CALL OPENF1(IDEVNI,FILNAM,0,IOK,KBIN,KBOUT, + +'Input file of file names', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) STOP + FILNAM = ' ' + CALL OPENF1(IDEVNO,FILNAM,1,IOK,KBIN,KBOUT, + +'Output file of passed file names', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) STOP + FILNAM = ' ' + CALL OPENF1(IDEVNF,FILNAM,1,IOK,KBIN,KBOUT, + +'Output file of failed file names', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) STOP + MN = 2 + MX = MAXWLN + LENGTH = 4 + CALL GETINT(MN,MX,LENGTH, + +'Word length', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) STOP + LENGTH = IVAL + MN = 1 + MX = 11 + LW = 7 + CALL GETINT(MN,MX,LW, + +'Number of diagonals to combine', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) STOP + LW = IVAL + XMN = 0.1 + XMX = 1.0 + CUT = 0.35 + CALL GETRL(XMN,XMX,CUT, + +'Cutoff score', + +VAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) STOP + CUT = VAL +C +C set initial values so we hash the first vector +C + FILNMV = ' ' + ICSITT = 0 + IPSITT = 0 + FILNAM = 'UNLIKELY FILE NAME' + ICSITE = ICSITT + IPSITE = IPSITT + IDE = (IDM-1)**LENGTH + CALL SETCN(CONSTS,LENGTH,IDM,LCONST) +C + IDEVE = 0 + 10 CONTINUE +C +C main loop: get the info we need from the experiment file +C for sequencing vector (job=1) we need: +C the reading +C its poor data pointers +C the vector +C the cloning site +C the primer position +C if poor data pointers are zero assume all ok +C if vector, cloning site, primer site not found skip +C +C for cloning vector (job=2) we need: +C the reading +C its sequencing vector pointers +C its poor data pointers +C the vector +C +C if sequencing vector pointers are zero use poor data pointers +C if poor data pointers are zero assume all ok +C if vector not found skip +C +C For both jobs shuffle sequence so we only process the unmarked segment +C then add on marker values at end +C Only hash vector if its different from the last one. +C +C Output: markers relative to the left end of the reading and name to file of +C passed file names. Plus a summary at the end. +C +C Make sure the experiment file is closed + CALL EXPKIL(IDEVE) +C Get next experiment file name + IOK = GNFFOF(IDEVNI,NAME) + IF(IOK.EQ.1) THEN + WRITE(KBOUT,*) + + 'Finished after processing',JGEL,' files and finding' + WRITE(KBOUT,*)ICB,' completely vector' + WRITE(KBOUT,*)IPG,' partly vector' + WRITE(KBOUT,*)ICG,' free of vector' + STOP + ELSE IF(IOK.EQ.2) THEN + CALL ERROM(KBOUT,'Empty line in file of file names') + GO TO 10 + ELSE IF(IOK.EQ.3) THEN + CALL ERROM(KBOUT,'Error reading file of file names') + GO TO 10 + END IF + IDEVE = EXPOPN(NAME) + IF(IDEVE.EQ.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,1) + CALL ERROM(KBOUT,'Error opening experiment file') + GO TO 10 + END IF + IDIMGI = MAXSEQ + IOK = REXGEL(IDEVE,SEQG,IDIMGI) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,2) + CALL ERROM(KBOUT,'Error getting gel reading') + GO TO 10 + END IF + JGEL = JGEL + 1 + WRITE(KBOUT,*)'>>>> Read number',JGEL,' length',IDIMGI,' ',NAME +C LONG ENOUGH ? + IF(IDIMGI.LT.LENGTH)THEN + CALL AERROR(KBOUT,IDEVNF,NAME,3) + CALL ERROM(KBOUT,'Gel reading too short to compare') + GO TO 10 + END IF + IDIMG = IDIMGI +C +C + IF(JOB.EQ.1) THEN +C +C Sequencing vector clipping +C +C get vector name, csite (cloning site), ipsite (primer site), +C poorl (poor data left mark), poorr +C + IOK = REXSVF(IDEVE,FILNMV) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,4) + CALL ERROM(KBOUT,'Error reading vector file name') + CALL ERROM(KBOUT,FILNMV) + GO TO 10 + END IF + IOK = REXSVC(IDEVE,ICSITT) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,5) + CALL ERROM(KBOUT,'Error reading cloning site') + GO TO 10 + END IF + IOK = REXSVQ(IDEVE,IPSITT) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,6) + CALL ERROM(KBOUT,'Error reading primer site') + GO TO 10 + END IF + IOK = REXPDP(IDEVE,POORL,POORR) + IF(IOK.NE.0) THEN +C CALL ERROM(KBOUT, +C + 'Error reading poor data positions, zero assumed') + END IF +C +C Decided to screen for primer in the poor data at the left end even +C though the program expects to ignore it: set poorl=0 + POORL = 0 +C +C IF ANY OF THESE ARE MISSING DO SOMETHING SENSIBLE !!!!!!!!!!!!! +C +C if filnam != current or icsitt != icsite or ipsitt != icsite +C then reorganise vector +C + IF((FILNMV.NE.FILNAM).OR. + + (ICSITT.NE.ICSITE).OR. + + (IPSITT.NE.IPSITE)) THEN + CALL OPENRS(IDEVV,FILNMV,IOK,LRECL,2) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,7) + CALL ERROM(KBOUT,'Error opening vector file') + CALL ERROM(KBOUT,FILNMV) + GO TO 10 + END IF + FILNAM = FILNMV + ICSITE = ICSITT + IPSITE = IPSITT + IDIMV = MAXSEQ + CALL ARRFIL(IDEVV,SEQV,IDIMV,KBOUT) + CLOSE(UNIT=IDEVV) +C check for contig header (should not be there) + IF(SEQV(20).EQ.'>') THEN + CALL SHFLCA(SEQV,MAXSEQ,21,1,IDIMV) + IDIMV = IDIMV - 20 + END IF + WRITE(KBOUT,*)'Vector length =',IDIMV +C make cloning site end of seq, then start of seq is icsite + 1 + CALL SQCOPY(SEQV(1),SEQV(IDIMV+1),ICSITE) +C if forward primer then need to complement vector + IF(IPSITE.GT.0) THEN + CALL SQREV(SEQV(ICSITE+1),IDIMV) + CALL SQCOM(SEQV(ICSITE+1),IDIMV) + END IF + CALL CONNUM(SEQV(ICSITE+1),SEQVI,IDIMV) + CALL ENCONC(SEQVI,IDIMV,POSN,WORDP,IDE,IDM,CONSTS,LENGTH, + + LCONST) + END IF +C +C now hash the reading +C +C clip reading so only the good data is processed +C +C WRITE(*,*)'POORL,POORR',POORL,POORR +C WRITE(*,*)(SEQG(K),K=1,IDIMG) +C WRITE(*,*)'IDIMG',IDIMG + IF(POORR.EQ.0) POORR = IDIMG + 1 + IF(POORL.GT.0) THEN + CALL SHFLCA(SEQG,MAXSEQ,POORL+1,1,POORR-1) + END IF + IDIMG = POORR - POORL - 1 +C WRITE(*,*)'IDIMG',IDIMG +C WRITE(*,*)(SEQG(K),K=1,IDIMG) + CALL CONNUM(SEQG,SEQGI,IDIMG) + CALL VCUT(SEQVI,IDIMV,POSN,WORDP,IDE,SEQGI,IDIMG,CONSTS, + + LENGTH,IDM,LCONST,HIST,MAXSEQ,KBOUT,CUT,LW,LC,RC,ICSITE, + + IPSITE) +C +C 1 nothing found either end lc = 0, rc = idimg+1 +C 2 primer only found lc = right end of primer, rc = idimg+1 +C 3 run into vector only lc = 0 rc = start of vector +C 4 primer found and vector run into lc = right end of primer, +C rc = start of vector +CC +C if rc = idimg+1 theres no vector at the right end of the reading +C +C WRITE(*,*)'LC,RC',LC,RC + IF(RC-LC+1.LT.20) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,9) + ICB = ICB + 1 + GO TO 10 + END IF +C +C note below we need to add on what weve cutoff +C + IF((LC.EQ.0).AND.(RC.EQ.IDIMG+1)) THEN +C +C No sequencing vector found +C + LCO = 0 + IRCO = IDIMGI + 1 + IOK = WEXSVP(IDEVE,LCO,IRCO) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,8) + GO TO 10 + END IF + ICG = ICG + 1 + ELSE IF((LC.NE.0).AND.(RC.NE.IDIMG+1)) THEN +C +C Primer found and run into sequencing vector +C + LCO = LC + POORL + IRCO = RC + POORL + IOK = WEXSVP(IDEVE,LCO,IRCO) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,8) + GO TO 10 + END IF + IPG = IPG + 1 + ELSE IF(LC.EQ.0) THEN +C +C Run into vector only +C + LCO = 0 + IRCO = RC + POORL + IOK = WEXSVP(IDEVE,LCO,IRCO) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,8) + GO TO 10 + END IF + IPG = IPG + 1 + ELSE IF(RC.EQ.IDIMG+1) THEN +C +C Primer only found +C + LCO = LC + POORL + IRCO = IDIMGI + 1 + IOK = WEXSVP(IDEVE,LCO,IRCO) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,8) + GO TO 10 + END IF + IPG = IPG + 1 + END IF + WRITE(IDEVNO,1005)NAME + 1005 FORMAT(A) +C +C + ELSE IF (JOB.EQ.2) THEN +C +C Cosmid clipping +C +C get vector file name, poorl (poor data left mark), poorr, +C svlclp (sequencing vector left clip position), svrclp +C + IOK = REXCVF(IDEVE,FILNMV) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,4) + CALL ERROM(KBOUT,'Error reading vector file name') + CALL ERROM(KBOUT,FILNMV) + GO TO 10 + END IF + IOK = REXPDP(IDEVE,POORL,POORR) + IF(IOK.NE.0) THEN +C CALL ERROM(KBOUT, +C + 'Error reading poor data positions, zero assumed') + END IF + IOK = REXSVP(IDEVE,SVLCLP,SVRCLP) + IF(IOK.NE.0) THEN +C CALL ERROM(KBOUT, +C + 'Error reading sequencing vector clip points, zero assumed') + END IF +C +C if filnam != current +C then reorganise vector +C + IF(FILNMV.NE.FILNAM) THEN + CALL OPENRS(IDEVV,FILNMV,IOK,LRECL,2) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,7) + CALL ERROM(KBOUT,'Error opening vector file') + CALL ERROM(KBOUT,FILNMV) + GO TO 10 + END IF + FILNAM = FILNMV + IDIMV = MAXSEQ + CALL ARRFIL(IDEVV,SEQV,IDIMV,KBOUT) + CLOSE(UNIT=IDEVV) +C check for contig header (should not be there) + IF(SEQV(20).EQ.'>') THEN + CALL SHFLCA(SEQV,MAXSEQ,21,1,IDIMV) + IDIMV = IDIMV - 20 + END IF + WRITE(KBOUT,*)'Vector length =',IDIMV + CALL CONNUM(SEQV,SEQVI,IDIMV) + CALL ENCONC(SEQVI,IDIMV,POSN,WORDP,IDE,IDM,CONSTS,LENGTH, + + LCONST) + END IF +C +C now hash the reading +C +C clip reading so only the good data is processed +C and set poorl and poorr to endpoints + POORL = MAX(POORL,SVLCLP) + I = IDIMG + 1 + IF(SVRCLP.GT.0) I = SVRCLP + IF(POORR.GT.0) I = MIN(I,POORR) + POORR = I +C +C WRITE(*,*)'POORL,POORR',POORL,POORR +C WRITE(*,*)(SEQG(K),K=1,IDIMG) +C WRITE(*,*)'IDIMG',IDIMG + IF(POORL.GT.0) THEN + CALL SHFLCA(SEQG,MAXSEQ,POORL+1,1,POORR-1) + END IF + IDIMG = POORR - POORL - 1 +C WRITE(*,*)'IDIMG',IDIMG +C WRITE(*,*)(SEQG(K),K=1,IDIMG) + CALL CONNUM(SEQG,SEQGI,IDIMG) + CALL VCUT(SEQVI,IDIMV,POSN,WORDP,IDE,SEQGI,IDIMG,CONSTS, + + LENGTH,IDM,LCONST,HIST,MAXSEQ,KBOUT,CUT,LW,LC,RC,0,0) + IF(LC.NE.0) THEN + IF(RC-LC+1.GE.IDIMG) THEN + ICB = ICB + 1 + CALL AERROR(KBOUT,IDEVNF,NAME,9) + ELSE + IPG = IPG + 1 + WRITE(IDEVNO,1005)NAME + END IF + LCO = LC + POORL + IRCO = RC + POORL + IOK = WEXCVP(IDEVE,LCO,IRCO) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,8) +C CALL ERROM(KBOUT, +C + 'Error writing cosmid vector positions') + GO TO 10 + END IF + GO TO 10 + END IF +C +C Try other strand +C + CALL SQCOPY(SEQG,SEQC,IDIMG) + CALL SQREV(SEQC,IDIMG) + CALL SQCOM(SEQC,IDIMG) + CALL CONNUM(SEQC,SEQGI,IDIMG) + CALL VCUT(SEQVI,IDIMV,POSN,WORDP,IDE,SEQGI,IDIMG,CONSTS, + + LENGTH,IDM,LCONST,HIST,MAXSEQ,KBOUT,CUT,LW,LC,RC,0,0) + IF(LC.NE.0) THEN + LC1 = IDIMG - RC + 1 + RC = IDIMG - LC + 1 + LC = LC1 + IF(RC-LC+1.GE.IDIMG) THEN + ICB = ICB + 1 + CALL AERROR(KBOUT,IDEVNF,NAME,9) + ELSE + WRITE(IDEVNO,1005)NAME + IPG = IPG + 1 + END IF + LCO = LC + POORL + IRCO = RC + POORL + IOK = WEXCVP(IDEVE,LCO,IRCO) + IF(IOK.NE.0) THEN + CALL AERROR(KBOUT,IDEVNF,NAME,8) +C CALL ERROM(KBOUT, +C + 'Error writing cosmid vector positions') + GO TO 10 + END IF + GO TO 10 + END IF + ICG = ICG + 1 + WRITE(IDEVNO,1005)NAME + GO TO 10 + ELSE +C +C unknown job !!!!!!!! +C + WRITE(*,*)'COCKUP' + END IF + GO TO 10 + END + SUBROUTINE VCUT(SEQV,IDIMV,POSN,WORDP,IDE,SEQH,IDIMH,CONSTS, + +LENGTH,IDM,LCONST,HIST,MAXSEQ,KBOUT,CUT,LW,LC,RC,ICSITE, + +IPSITE) + INTEGER SEQV(IDIMV),SEQH(IDIMH) + INTEGER POSN(IDIMV),WORDP(IDE),CONSTS(0:LCONST) + INTEGER RC + REAL HIST(-MAXSEQ:MAXSEQ) + EXTERNAL NCODEA +C CALL BUSY(KBOUT) + CALL FILLR(HIST(LENGTH-IDIMV),IDIMH+IDIMV+1,0.) + DO 20 I = 1,IDIMH-LENGTH+1 + J = NCODEA(SEQH(I),LENGTH,CONSTS,IDM,LCONST) + IF(J.NE.0)THEN + J1 = WORDP(J) + IF(J1.NE.0)THEN + K = I - J1 + HIST(K) = HIST(K) + 1. +10 CONTINUE + J2 = J1 + J1 = POSN(J2) + IF(J1.NE.0)THEN + K = I - J1 + HIST(K) = HIST(K) + 1. + GO TO 10 + END IF + END IF + END IF +20 CONTINUE + CALL PHIST(HIST,IDIMV,IDIMH,LENGTH,MAXSEQ) + IF(ICSITE.NE.0) THEN +C +C look for primer region +C + CALL FCUT(HIST,IDIMV,IDIMH,LENGTH, + + MAXSEQ,CUT,LW,LC,RC,IPSITE,1) + IF (LC.EQ.0) THEN +C WRITE(KBOUT,*)' ***** No primer site found ********' + ELSE +C +C if primer found we want to know where it ends so set lc=rc +C + LC = RC + END IF +C +C look for running into vector at cloning site +C + CALL FCUT(HIST,IDIMV,IDIMH,LENGTH, + + MAXSEQ,CUT,LW,LCR,IRCR,IPSITE,2) +C +C set right cut to 1 past the end of the sequence +C if some vector found we set rc to where it starts +C + RC = IDIMH + 1 + IF(LCR.GT.0) THEN + RC = LCR + END IF +C +C so the outcomes are: +C 1 nothing found either end lc = 0, rc = idimg+1 +C 2 primer only found lc = right end of primer, rc = idimg+1 +C 3 run into vector only lc = 0 rc = start of vector +C 4 primer found and vector run into lc = right end of primer, +C rc = start of vector +C +C + ELSE +C +C look for cosmid vector +C + CALL FCUT(HIST,IDIMV,IDIMH,LENGTH, + + MAXSEQ,CUT,LW,LC,RC,IPSITE,3) + IF(LC.GT.0) THEN +C WRITE(KBOUT,*) +C +'>>>>>>>>>>>>>>>>>>>>>>>>diagonal found' + RETURN + END IF + END IF + END + SUBROUTINE PHIST(HIST,IDIMV,IDIMH,LENGTH,MAXSEQ) + REAL HIST(-MAXSEQ:MAXSEQ) + IF(IDIMV.GE.IDIMH) THEN + D = LENGTH + DO 10 I=LENGTH-IDIMV,IDIMH-IDIMV-1 + HIST(I) = HIST(I)/D + D = D + 1 + 10 CONTINUE + D = IDIMH + DO 20 I=IDIMH-IDIMV,0 + HIST(I) = HIST(I)/D + 20 CONTINUE + D = IDIMH - 1 + DO 30 I=1,IDIMH-LENGTH + HIST(I) = HIST(I)/D + D = D - 1 + 30 CONTINUE + ELSE + D = LENGTH + DO 40 I=LENGTH-IDIMV,-1 + HIST(I) = HIST(I)/D + D = D + 1 + 40 CONTINUE + D = IDIMV + DO 50 I=0,IDIMH-IDIMV + HIST(I) = HIST(I)/D + 50 CONTINUE + D = IDIMV - 1 + DO 60 I=IDIMH-IDIMV+1,IDIMH-LENGTH + HIST(I) = HIST(I)/D + D = D - 1 + 60 CONTINUE + END IF + END + SUBROUTINE FCUT(HIST,IDIMV,IDIMH,LENGTH, + + MAXSEQ,CUT,LW,LC,RC,PSITE,JOB) + REAL HIST(-MAXSEQ:MAXSEQ) + INTEGER RC,PSITE + PARAMETER (MINVEC = 6) +C MINVEC is minimum length of cloning vector to be reported +C PSITE is primer site + LC = 0 + RC = 0 + DMAX = 0. +C If job = 1 look for cloning site from psite to the end of the vector +C if job = 2 look for vector in the rest of the sequence +C If job = 3 look for cosmid vector in whole of sequence +C We discard lc to rc inclusive, lc=0 means discard nothing + IF(JOB.EQ.1) THEN + I1 = LENGTH - IDIMV + I2 = -ABS(PSITE) + ELSE IF(JOB.EQ.2) THEN + I1 = -ABS(PSITE) + I2 = IDIMH - LENGTH + ELSE IF(JOB.EQ.3) THEN + I1 = LENGTH - IDIMV + MINVEC + I2 = IDIMH - LENGTH - MINVEC + ELSE + WRITE(*,*)'Error in FCUT' + RETURN + END IF +C WRITE(*,*)'LOOKING AT ',I1,I2 + DO 10 I=I1,I2 + DT = HIST(I) + IF(DT.GT.DMAX) THEN + DMAX = DT + ID = I + END IF + 10 CONTINUE + D = 0. + DO 35 I=MAX(ID-LW/2,LENGTH-IDIMV), + + MIN(ID+LW/2,IDIMH-LENGTH) + D = D + HIST(I) + 35 CONTINUE +C WRITE(*,*)'Best diagonal, score and local sum',ID,DMAX,D +C WRITE(*,1000)ID,DMAX,D + 1000 FORMAT(I6,2F10.3) + IF(D.LT.CUT) RETURN + IF(IDIMV.GE.IDIMH) THEN + IF(ID.GE.0) THEN + LC = ID + 1 + RC = IDIMH + ELSE + LC = 1 + RC = MIN(IDIMH,IDIMV + ID) + END IF + ELSE + IF(ID.GE.0) THEN + LC = ID + 1 + RC = MIN(ID+IDIMV,IDIMH) + ELSE + LC = 1 + RC = ID + IDIMV + END IF + END IF + WRITE(*,*)' Discard ',LC, ' to ',RC + END + INTEGER FUNCTION GNFFOF(IDEV,NAME) + CHARACTER NAME*(*) + EXTERNAL NOTLR +C +C routine to read a file of file names and return a name +C deals with leading spaces and trims names at first space +C after name: eg ' fred is a bum' is returned as 'fred' +C needed because file names can contain spaces (not our file names!) +C and the open statement expects the names to match precisely +C +C return 0 = ok, 2 = empty line in file, 3 = error in read, 1 = end of file +C + READ(IDEV,1000,ERR=100,END=200)NAME + 1000 FORMAT(A) +C +C get first non space position +C + LENGTH = LEN(NAME) + I = NOTLR(NAME,LENGTH,' ') +C empty line ? + IF(I.EQ.0) THEN + GNFFOF = 2 + RETURN + END IF +C now want first space after I + J = INDEX(NAME(I+1:),' ') + IF(J.EQ.0) THEN + J = LENGTH + ELSE + J = J + I - 1 + END IF + CALL SHFTLS(NAME,I,1,J) + NAME(J-I+2:) = ' ' + GNFFOF = 0 + RETURN + 100 CONTINUE + GNFFOF = 3 + RETURN + 200 CONTINUE + GNFFOF = 1 + END + SUBROUTINE SHFTLS(STRING,FROMS,TO,FROME) + CHARACTER STRING*(*) + INTEGER FROMS,TO,FROME +C +C shift a string left from froms to to +C + J = TO + DO 10 I=FROMS,FROME + STRING(J:J) = STRING(I:I) + J = J + 1 + 10 CONTINUE + END + SUBROUTINE SHFLCA(STRING,MAXAR,FROMS,TO,FROME) + CHARACTER STRING(MAXAR) + INTEGER FROMS,FROME,TO +C +C shift left from from to to +C + J = TO + DO 10 I=FROMS,FROME + STRING(J) = STRING(I) + J = J + 1 + 10 CONTINUE + END + SUBROUTINE AERROR(IDEVS,IDEVF,NAME,IERR) + CHARACTER NAME*(*) +C +C handle errors for assembly +C +C errors are: +C 0 file not found +C 1 read too short +C 2 failed to align and not entered +C 3 failed on entry +C 4 failed to align but entered + WRITE(IDEVF,1000)NAME(1:INDEX(NAME,' ')),IERR + 1000 FORMAT(A,I2) + CALL ERROM(IDEVS,'Failed reading written to error file') + END diff --git a/staden.login b/staden.login new file mode 100644 index 0000000..2e43183 --- /dev/null +++ b/staden.login @@ -0,0 +1,158 @@ +# +# Setup file for Staden software running on a Sun +# This setup for c-shell (csh) users +# +# This file should be source'd from your .login +# assuming the environmental variable STADENROOT has been set up +# to point to the root directory for the staden software +# +# e.g. +# setenv STADENROOT /home/BioSW/staden +# source $STADENROOT/staden.login +# +# +#echo 'Setting up the Staden software environment...' + +set path = ($path $STADENROOT/bin) + +setenv STADTABL $STADENROOT/tables +setenv STADUSER $STADENROOT/userdata +setenv STADHELP $STADENROOT/help + +# The default is 'vi' but this causes the X versions to hang +setenv SEQEDT 'xterm -e vi' + +# +# files for nip +# +setenv ECRIBS $STADTABL/PERCPTRON.WTS +setenv EUKRIBS $STADTABL/RIBSEUKAR.WTS +setenv INTRONS $STADTABL/INTRON.WTS +setenv ECPROMS $STADTABL/PROKPROMS.WTS +setenv RENZYM4 $STADTABL/RENZYM.4 +setenv RENZYM6 $STADTABL/RENZYM.6 +setenv RENZYMAL $STADTABL/RENZYM.ALL +setenv HELPNIP $STADHELP/nip_help +setenv NIPHELP $STADHELP/NIP.HLP +setenv NIPHPNT $STADHELP/NIP.PNT +setenv NIPMARG $STADTABL/NIP.MRG + + +# files for nipf + +setenv NIPFMARG $STADTABL/NIPF.MRG + + +# +# files for sip +# +setenv HELPSIP $STADHELP/sip_help +setenv SIPHELP $STADHELP/SIP.HLP +setenv SIPHPNT $STADHELP/SIP.PNT +setenv SIPMARG $STADTABL/SIP.MRG +setenv PROTMAT $STADTABL/PROTMAT.DAT + +# +# files for sap +# +setenv HELPSAP $STADHELP/sap_help +setenv SAPHELP $STADHELP/SAP.HLP +setenv SAPHPNT $STADHELP/SAP.PNT +setenv SAPMARG $STADTABL/SAP.MRG + +# +# files for bap +# +setenv HELPBAP $STADHELP/bap_help +setenv BAPHELP $STADHELP/BAP.HLP +setenv BAPHPNT $STADHELP/BAP.PNT +setenv BAPMARG $STADTABL/BAP.MRG + +# +# files for dap +# +setenv HELPDAP $STADHELP/dap_help +setenv DAPHELP $STADHELP/DAP.HLP +setenv DAPHPNT $STADHELP/DAP.PNT +setenv DAPMARG $STADTABL/DAP.MRG +setenv TAGDB $STADTABL/TAGDB + +# +# files for pip +# +setenv PIPMARG $STADTABL/PIP.MRG +setenv PROTGRP $STADTABL/PROTEIN.GRP +setenv PROTALL $STADTABL/PROTEIN.ALL +setenv ROBSON $STADTABL/ROBSON.WTS +setenv HELPPIP $STADHELP/pip_help +setenv PIPHELP $STADHELP/PIP.HLP +setenv PIPHPNT $STADHELP/PIP.PNT + +# +# files for mep +# +setenv MEPMARG $STADTABL/MEP.MRG +setenv HELPMEP $STADHELP/mep_help +setenv MEPHELP $STADHELP/MEP.HLP +setenv MEPHPNT $STADHELP/MEP.PNT + +# +# files for rep +# +setenv ALUNAMES $STADTABL/alus/files + + + +# +# miscellaneous files +# +setenv GIPMEM $STADHELP/gip_help +setenv HELPSTADEN $STADHELP/staden_help +# +# Typing "staden" will list all the program names +# +alias staden 'cat $STADHELP/stadenp_help' +# +# vector sequences +# +setenv M13MP18_VECTOR $STADUSER/m13mp18.vec +setenv PUC18_VECTOR $STADUSER/puc18.vec +setenv BLUE_VECTOR $STADUSER/blue.vec + +setenv PROSITE /pubseq/pubseq/seqlibs/prosite +setenv PROSITENAMES /pubseq/pubseq/seqlibs/prosite/prosite.nam + + + + +# +# Help X11 find where things are +# +if ( $?XFILESEARCHPATH ) then + setenv XFILESEARCHPATH ${STADHELP}/%N%S:${STADTABL}/%N%S:${XFILESEARCHPATH} +else + setenv XFILESEARCHPATH ${STADHELP}/%N%S:${STADTABL}/%N%S:/usr/lib/X11/%T/%N%S +endif + +# +# Find manual pages +# +if ( $?MANPATH ) then + setenv MANPATH ${MANPATH}:${STADENROOT} +else + setenv MANPATH /usr/man:${STADENROOT} +endif + +# +# For gip, specify default digitizer port +# +setenv DIGITIZER /dev/ttyb + + +# +# Sequence databases +# + +source $STADTABL/libraries.config.csh + + diff --git a/staden.profile b/staden.profile new file mode 100644 index 0000000..5800e35 --- /dev/null +++ b/staden.profile @@ -0,0 +1,144 @@ +# +# Setup file for Staden software running on a Sun +# This setup for Bourne shell (sh) users +# +# This file should be source'd from your .profile +# assuming the environmental variable STADENROOT has been set up +# to point to the root directory for the staden software +# +# e.g. +# STADENROOT=/home/BioSW/staden; export STADENROOT +# . $STADENROOT/staden.setup +# +# +#echo 'Setting up the Staden software environment...' + +PATH=$PATH:$STADENROOT/bin; export PATH + +STADTABL=$STADENROOT/tables; export STADTABL +STADUSER=$STADENROOT/userdata; export STADUSER +STADHELP=$STADENROOT/help; export STADHELP + + +# +# files for nip +# +ECRIBS=$STADTABL/PERCPTRON.WTS; export ECRIBS +EUKRIBS=$STADTABL/RIBSEUKAR.WTS;export EUKRIBS +INTRONS=$STADTABL/INTRON.WTS; export INTRONS +ECPROMS=$STADTABL/PROKPROMS.WTS;export ECPROMS +RENZYM4=$STADTABL/RENZYM.4; export RENZYM4 +RENZYM6=$STADTABL/RENZYM.6; export RENZYM6 +RENZYMAL=$STADTABL/RENZYM.ALL; export RENZYMAL +HELPNIP=$STADHELP/nip_help; export HELPNIP +NIPHELP=$STADHELP/NIP.HLP; export NIPHELP +NIPHPNT=$STADHELP/NIP.PNT; export NIPHPNT +NIPMARG=$STADTABL/NIP.MRG; export NIPMARG + +# +# files for sip +# +HELPSIP=$STADHELP/sip_help; export HELPSIP +SIPHELP=$STADHELP/SIP.HLP; export SIPHELP +SIPHPNT=$STADHELP/SIP.PNT; export SIPHPNT +SIPMARG=$STADTABL/SIP.MRG; export SIPMARG +PROTMAT=$STADTABL/PROTMAT.DAT; export PROTMAT + +# +# files for sap +# +HELPSAP=$STADHELP/sap_help; export HELPSAP +SAPHELP=$STADHELP/SAP.HLP; export SAPHELP +SAPHPNT=$STADHELP/SAP.PNT; export SAPHPNT +SAPMARG=$STADTABL/SAP.MRG; export SAPMARG + +# +# files for bap +# +HELPBAP=$STADHELP/bap_help; export HELPBAP +BAPHELP=$STADHELP/BAP.HLP; export BAPHELP +BAPHPNT=$STADHELP/BAP.PNT; export BAPHPNT +BAPMARG=$STADTABL/BAP.MRG; export BAPMARG + +# +# files for dap +# +HELPDAP=$STADHELP/dap_help; export HELPDAP +DAPHELP=$STADHELP/DAP.HLP; export DAPHELP +DAPHPNT=$STADHELP/DAP.PNT; export DAPHPNT +DAPMARG=$STADTABL/DAP.MRG; export DAPMARG +TAGDB=$STADTABL/TAGDB; export TAGDB + +# +# files for pip +# +PIPMARG=$STADTABL/PIP.MRG; export PIPMARG +PROTGRP=$STADTABL/PROTEIN.GRP; export PROTGRP +PROTALL=$STADTABL/PROTEIN.ALL; export PROTALL +ROBSON=$STADTABL/ROBSON.WTS; export ROBSON +HELPPIP=$STADHELP/pip_help; export HELPPIP +PIPHELP=$STADHELP/PIP.HLP; export PIPHELP +PIPHPNT=$STADHELP/PIP.PNT; export PIPHPNT + +# +# files for mep +# +MEPMARG=$STADTABL/MEP.MRG; export MEPMARG +HELPMEP=$STADHELP/mep_help; export HELPMEP +MEPHELP=$STADHELP/MEP.HLP; export MEPHELP +MEPHPNT=$STADHELP/MEP.PNT; export MEPHPNT + +# +# files for rep +# +ALUNAMES=$STADTABL/alus/files; export ALUNAMES + + +# +# miscellaneous files +# +GIPMEM=$STADHELP/gip_help; export GIPMEM +HELPSTADEN=$STADHELP/staden_help;export HELPSTADEN + +# +# vector sequences +# +M13MP18_VECTOR=$STADUSER/m13mp18.vec; export M13MP18_VECTOR +PUC18_VECTOR=$STADUSER/puc18.vec; export PUC18_VECTOR +BLUE_VECTOR=$STADUSER/blue.vec; export BLUE_VECTOR + + + +# +# Sequence databases (currently embl cdrom format only) +# + +# The file SEQUENCELIBRARIES lists the available libraries +# and the *DESCRP files contain the names of each libraries files +# +SEQUENCELIBRARIES=$STADTABL/SEQUENCELIBRARIES; export SEQUENCELIBRARIES +EMBLLIBDESCRP=$STADTABL/EMBLLIBDESCRP; export EMBLLIBDESCRP +SWISSLIBDESCRP=$STADTABL/SWISSLIBDESCRP; export SWISSLIBDESCRP + + +# +# Help X11 find where things are +# +XFILESEARCHPATH=$STADHELP/%N%S:$STADTABL/%N%S:${XFILESEARCHPATH-/usr/lib/X11/%T/%N%S} +export XFILESEARCHPATH + +# +# Find manual pages +# +MANPATH=${MANPATH-/usr/man}:$STADENROOT; export MANPATH + +# +# For gip, specify default digitizer port +# +DIGITIZER=/dev/ttyb; export DIGITIZER + +# +# Sequence databases +# + +. $STADTABL/libraries.config.sh diff --git a/tables/ATPASE.DAT b/tables/ATPASE.DAT new file mode 100644 index 0000000..f3bc0c3 --- /dev/null +++ b/tables/ATPASE.DAT @@ -0,0 +1,176 @@ +ID ATPase +XX +DT 10-NOV-1982 FIRST ENTRY +XX +DE +XX +KW +XX +FH KEY FROM TO DESCRIPTION +FH +FT CDS 576 968 1 0 391 +FT CDS 977 1792 a 1 814 +FT CDS 1839 2081 c 6-12 242 +FT CDS 2140 2610 b 2 439 +FT CDS 2625 3158 DELTA 1 532 +FT CDS 3171 4712 ALPHA 3 1540 +FT CDS 4763 5626 GAMMA 1 864 +FT CDS 5653 7035 BETA 3 1381 +FT CDS 7056 7400 EPSILON 1 343 +FT CDS3 576 968 1 0 391 +FT CDS2 977 1792 a 1 814 +FT CDS3 1839 2081 c 6-12 242 +FT CDS1 2140 2610 b 2 439 +FT CDS3 2625 3158 DELTA 1 532 +FT CDS3 3171 4712 ALPHA 3 1540 +FT CDS2 4763 5626 GAMMA 1 864 +FT CDS1 5653 7035 BETA 3 1381 +FT CDS3 7056 7400 EPSILON 1 343 +FT STOK 576 968 1 0 391 +FT STOK 977 1792 1 1 814 +FT STOK 1839 2081 6 6-12 242 +FT STOK 2140 2610 2 2 439 +FT STOK 2625 3158 1ELTA 1 532 +FT STOK 3171 4712 3LPHA 3 1540 +FT STOK 4763 5626 1AMMA 1 864 +FT STOK 5653 7035 3ETA 3 1381 +FT STOK 7056 7400 1PSILON 1 343 +XX +SQ + CCGGTTAGAC TGTTAACAAC AACCAGGTTT TCTACTGATA TAACTGGTTA CATTTAACGC + CACGTTCACT CTTTTGCATC AACAAGATAA CGTGGCTTTT TTTGGTAAGC AGAAAATAAG + TCATTAGTGA AAATATCAGT CTGCTAAAAA TCGGCGCTAA GAACCATCAT TGGCTGTTAA + AACAGTTATT AAAAATGTCA ATGGGTGGTT TTTGTTGTGT AAATGTCATT TATTAAACAG + GTATCTGTTT TTAGACTGAA ATATCATAAA CTTGCAAGGC ATCATTTGCC AAGTAAATAA + ATATGCTGTG CGCGAACATG CGCAATATGT GATCTGAAGC ACGCTTTATC ACCAGTGTTT + ACGCGTTATT TACAGTTTTT CATGATCGAA CAGGGTTAGC AGAAAAGTCG CAATTGTATG + CACTGGAAAA ATATTTAAAC ATTTATTCAC CTTTTGGCTA CTTATTGTTT GAAATCACGG + GGGCGCACCG TATAATTTGA CCGCTTTTTG ATGCTTGACT CTAAGCCTTA AAGAAAGTTT + TATACGACAC GCGGCATACC TCGAAGGGAG CAGGAGTGAA AAACGTGATG TCTGTGTCGC + TCGTGAGTCG AAACGTTGCT CGGAAGCTTC TGCTCGTTCA GTTACTGGTG GTGATAGCAA + GTGGATTGCT GTTCAGCCTC AAAGACCCCT TCTGGGGCGT CTCTGCAATA AGCGGGGGCC + TGGCAGTCTT TCTGCCTAAC GTTTTGTTTA TGATATTTGC CTGGCGTCAC CAGGCGCATA + CACCAGCGAA AGGCCGGGTG GCCTGGACAT TCGCATTTGG CGAAGCTTTC AAAGTTCTGG + CGATGTTGGT GTTACTGGTG GTGGCGTTGG CGGTTTTAAA GGCGGTATTC TTGCCGCTGA + TCGTTACGTG GGTTTTGGTG CTGGTGGTTC AGATACTGGC ACCGGCTGTA ATTAACAACA + AAGGGTAAAA GGCATCATGG CTTCAGAAAA TATGACGCCG CAGGATTACA TAGGACACCA + CCTGAATAAC CTTCAGCTGG ACCTGCGTAC ATTCTCGCTG GTGGATCCAC AAAACCCCCC + AGCCACCTTC TGGACAATCA ATATTGACTC CATGTTCTTC TCGGTGGTGC TGGGTCTGTT + GTTCCTGGTT TTATTCCGTA GCGTAGCCAA AAAGGCGACC AGCGGTGTGC CAGGTAAGTT + TCAGACCGCG ATTGAGCTGG TGATCGGCTT TGTTAATGGT AGCGTGAAAG ACATGTACCA + TGGCAAAAGC AAGCTGATTG CTCCGCTGGC CCTGACGATC TTCGTCTGGG TATTCCTGAT + GAACCTGATG GATTTACTGC CTATCGACCT GCTGCCGTAC ATTGCTGAAC ATGTACTGGG + TCTGCCTGCA CTGCGTGTGG TTCCGTCTGC GGACGTGAAC GTAACGCTGT CTATGGCACT + GGGCGTATTT ATCCTGATTC TGTTCTACAG CATCAAAATG AAAGGCATCG GCGGCTTCAC + GAAAGAGTTG ACGCTGCAGC CGTTCAATCA CTGGGCGTTC ATTCCTGTCA ACTTAATCCT + TGAAGGGGTA AGCCTGCTGT CCAAACCAGT TTCACTCGGT TTGCGACTGT TCGGTAACAT + GTATGCCGGT GAGCTGATTT TCATTCTGAT TGCTGGTCTG TTGCCGTGGT GGTCACAGTG + GATCCTGAAT GTGCCGTGGG CCATTTTCCA CATCCTGATC ATTACGCTGC AAGCCTTCAT + CTTCATGGTT CTGACGATCG TCTATCTGTC GATGGCGTCT GAAGAACATT AATTTACCAA + CACTACTACG TTTTAACTGA AACAAACTGG AGACTGTCAT GGAAAACCTG AATATGGATC + TGCTGTACAT GGCTGCCGCT GTGATGATGG GTCTGGCGGC AATCGGTGCT GCGATCGGTA + TCGGCATCCT CGGGGGTAAA TTCCTGGAAG GCGCAGCGCG TCAACCTGAT CTGATTCCTC + TGCTGCGTAC TCAGTTCTTT ATCGTTATGG GTCTGGTGGA TGCTATCCCG ATGATCGCTG + TAGGTCTGGG TCTGTACGTG ATGTTCGCTG TCGCGTAGTA AGCGTTGCTT TTATTTAAAG + AGCAATATCA GAACGTTAAC TAAATAGAGG CATTGTGCTG TGAATCTTAA CGCAACAATC + CTCGGCCAGG CCATCGCGTT TGTCCTGTTC GTTCTGTTCT GCATGAAGTA CGTATGGCCG + CCATTAATGG CAGCCATCGA AAAACGTCAA AAAGAAATTG CTGACGGCCT TGCTTCCGCA + GAACGAGCAC ATAAGGACCT TGACCTTGCA AAGGCCAGCG CGACCGACCA GCTGAAAAAA + GCGAAAGCGG AAGCCCAGGT AATCATCGAG CAGGCGAACA AACGCCGCTC GCAGATTCTG + GACGAAGCGA AAGCTGAGGC AGAACAGGAA CGTACTAAAA TCGTGGCCCA GGCGCAGGCG + GAAATTGAAG CCGAGCGTAA ACGTGCCCGT GAAGAGCTGC GTAAGCAAGT TGCTATCCTG + GCTGTTGCTG GCGCCGAGAA GATCATCGAA CGTTCCGTGG ATGAAGCTGC TAACAGCGAC + ATCGTGGATA AACTTGTCGC TGAACTGTAA GGAGGGAGGG GCTGATGTCT GAATTTATTA + CGGTAGCTCG CCCCTACGCC AAAGCAGCTT TTGACTTTGC CGTCGAACAC CAAAGTGTAG + AACGCTGGCA GGACATGCTG GCGTTTGCCG CCGAGGTAAC CAAAAACGAA CAAATGGCAG + AGCTTCTCTC TGGCGCGCTT GCGCCAGAAA CGCTCGCCGA GTCGTTTATC GCAGTTTGTG + GTGAGCAACT GGACGAAAAC GGTCAGAACC TGATTCGGGT TATGGCTGAA AATGGTCGTC + TTAACGCGCT CCCGGATGTT CTGGAGCAGT TTATTCACCT GCGTGCCGTG AGTGAGGCTA + CCGCTGAGGT AGACGTCATT TCCGCTGCCG CACTGAGTGA ACAACAGCTC GCGAAAATTT + CTGCTGCGAT GGAAAAACGT CTGTCACGCA AAGTTAAGCT GAATTGCAAA ATCGATAAGT + CTGTAATGGC AGGCGTTATC ATCCGAGCGG GTGATATGGT CATTGATGGC AGCGTACGCG + GTCGTCTTGA GCGCCTTGCA GACGTCTTGC AGTCTTAAGG GGACTGGAGC ATGCAACTGA + ATTCCACCGA AATCAGCGAA CTGATCAAGC AGCGCATTGC TCAGTTCAAT GTTGTGAGTG + AAGCTCACAA CGAAGGTACT ATTGTTTCTG TAAGTGACGG TGTTATCCGC ATTCACGGCC + TGGCCGATTG TATGCAGGGT GAAATGATCT CCCTGCCGGG TAACCGTTAC GCTATCGCAC + TGAACCTCGA GCGCGACTCT GTAGGTGCGG TTGTTATGGG TCCGTACGCT GACCTTGCCG + AAGGCATGAA AGTTAAGTGT ACTGGCCGTA TCCTGGAAGT TCCGGTTGGC CGTGGCCTGC + TGGGCCGTGT GGTTAACACT CTGGGTGCAC CAATCGACGG TAAAGGTCCG CTGGATCACG + ACGGCTTCTC TGCTGTAGAA GCAATCGCTC CGGGCGTTAT CGAACGTCAG TCCGTAGATC + AGCCGGTACA GACCGGTTAT AAAGCCGTTG ACTCCATGAT CCCAATCGGT CGTGGTCAGC + GTGAATTGAT CATCGGTGAC CGTCAGACAG GTAAAACCGC ACTGGCTATC GATGCCATCA + TCAACCAGCG CGATTCCGGT ATCAAATGTA TCTATGTCGC TATCGGCCAG AAAGCGTCCA + CCATTTCTAA CGTGGTACGT AAACTGGAAG AGCACGGCGC ACTGGCTAAC ACCATCGTTG + TGGTAGCAAC CGCGTCTGAA TCCGCTGCAC TGCAATACCT GGCACGTATG CCGGTTGCGC + TAATGGGCGA ATACTTCCGT GACCGCGGTG AAGATGCGCT GATCATTTAC GATGACCTGT + CTAAACAGGC TGTTGCTTAC CGTCAGATCT CCCTGCTGCT CCGTCGTCCG CCAGGACGTG + AAGCATTCCC GGGCGACGTT TTCTACCTCC ACTCTCGTCT GCTGGAGCGT GCTGCACGTG + TTAACGCCGA ATACGTTGAA GCCTTCACCA AAGGTGAAGT GAAAGGGAAA ACCGGTTCTC + TGACCGCACT GCCGATTATC GAAACTCAGG CGGGTGACGT TTCTGCGTTC GTTCCGACCA + ACGTAATCTC CATTACCGAT GGTCAGATCT TCCTGGAAAC CAACCTGTTC AACGCCGGTA + TTCGTCCTGC GGTTAACCCG GGTATTTCCG TATCCCGTGT TGGTGGTGCA GCACAGACCA + AGATCATGAA AAAACTGTCC GGTGGTATCC GTACCGCTCT GGCACAGTAT CGTGAACTGG + CAGCGTTCTC TCAGTTTGCA TCCGACCTTG ACGATGCAAC ACGTAAGCAG CTTGACCACG + GTCAGAAAGT GACCGAACTG CTGAAACAGA AACAGTATGC GCCGATGTCC GTTGCGCAGC + AGTCTCTGGT TCTGTTCGCA GCAGAACGTG GTTACCTGGC GGATGTTGAA CTGTCGAAAA + TTGGCAGCTT CGAAGCCGCT CTGCTGGCTT ACGTCGACCG TGATCACGCT CCGTTGATGC + AAGAGATCAA CCAGACCGGT GGCTACAACG ACGAAATCGA AGGCAAGCTG AAAGGCATCC + TCGATTCCTT CAAAGCAACC CAATCCTGGT AACGTCTGGC GGCTTGCCTT AGGGCAGGCC + GCAAGGCATT GAGGAGAAGC TCATGGCCGG CGCAAAAGAG ATACGTAGTA AGATCGCAAG + CGTCCAGAAC ACGCAAAAGA TCACTAAAGC GATGGAGATG GTCGCCGCTT CCAAAATGCG + TAAATCGCAG GATCGCATGG CGGCCAGCCG TCCTTATGCA GAAACCATGC GCAAAGTGAT + TGGTCACCTT GCACACGGTA ATCTGGAATA TAAGCACCCT TACCTGGAAG ACCGCGACGT + TAAACGCGTG GGCTACCTGG TGGTGTCGAC CGACCGTGGT TTGTGCGGTG GTTTGAACAT + TAACCTGTTC AAAAAACTGC TGGCGGAAAT GAAGACCTGG ACCGACAAAG GCGTTCAATG + CGACCTCGCA ATGATCGGCT CGAAAGGCGT GTCGTTCTTC AACTCCGTGG GCGGCAATGT + TGTTGCCCAG GTCACCGGCA TGGGGGATAA CCCTTCCCTG TCCGAACTGA TCGGTCCGGT + AAAAGTGATG TTGCAGGCCT ACGACGAAGG CCGTCTGGAC AAGCTTTACA TTGTCAGCAA + CAAATTTATT AACACCATGT CTCAGGTTCC GACCATCAGC CAGCTGCTGC CGTTACCGGC + ATCAGATGAT GATGATCTGA AACATAAATC CTGGGATTAC CTGTACGAAC CCGATCCGAA + GGCGTTGCTG GATACCCTGC TGCGTCGTTA TGTCGAATCT CAGGTTTATC AGGGCGTGGT + TGAAAACCTG GCCAGCGAGC AGGCCGCCCG TATGGTGGCG ATGAAAGCCG CGACCGACAA + TGGCGGCAGC CTGATTAAAG AGCTGCAGTT GGTATACAAC AAAGCTCGTC AGGCCAGCAT + TACTCAGGAA CTCACCGAGA TCGTCTCGGG GGCCGCCGCG GTTTAAACAG GTTATTTCGT + AGAGGATTTA AGATGGCTAC TGGAAAGATT GTCCAGGTAA TCGGCGCCGT AGTTGACGTC + GAATTCCCTC AGGATGCCGT ACCGCGCGTG TACGATGCTC TTGAGGTGCA AAATGGTAAT + GAGCGTCTGG TGCTGGAAGT TCAGCAGCAG CTCGGCGGCG GTATCGTACG TACCATCGCA + ATGGGTTCCT CCGACGGTCT GCGTCGCGGT CTGGATGTAA AAGACCTCGA ACACCCGATT + GAAGTCCCGG TAGGTAAAGC GACTCTGGGC CGTATCATGA ACGTACTGGG TGAACCGGTC + GACATGAAAG GCGAGATCGG TGAAGAAGAG CGTTGGGCGA TTCACCGCGC AGCACCTTCC + TACGAAGAGC TGTCAAACTC TCAGGAACTG CTGGAAACCG GTATCAAAGT TATCGACCTG + ATGTGTCCGT TCGCTAAGGG CGGTAAAGTT GGTCTGTTCG GTGGTGCGGG TGTAGGTAAA + ACCGTAAACA TGATGGAGCT CATTCGTAAC ATCGCGATCG AGCACTCCGG TTACTCTGTG + TTTGCGGGCG TAGGTGAACG TACTCGTGAG GGTAACGACT TCTACCACGA AATGACCGAC + TCCAACGTTA TCGACAAAGT ATCCCTGGTG TATGGCCAGA TGAACGAGCC GCCGGGAAAC + CGTCTGCGCG TTGCTCTGAC CGGTCTGACC ATGGCTGAGA AATTCCGTGA CGAAGGTCGT + GACGTTCTGC TGTTCGTTGA CAACATCTAT CGTTACACCC TGGCCGGTAC GGAAGTATCC + GCACTGCTGG GCCGTATGCC TTCAGCGGTA GGTTATCAGC CGACCCTGGC GGAAGAGATG + GGCGTTCTGC AGGAACGTAT CACCTCCACC AAAACTGGTT CTATCACCTC CGTACAGGCA + GTATACGTAC CTGCGGATGA CTTGACTGAC CCGTCTCCGG CAACCACCTT TGCGCACCTT + GACGCAACCG TGGTACTGAG CCGTCAGATC GCGTCTCTGG GTATCTACCC GGCCGTTGAC + CCGCTGGACT CCACCAGCCG TCAGCTGGAC CCGCTGGTGG TTGGTCAGGA ACACTACGAC + ACCGCGCGTG GCGTTCAGTC CATCCTGCAA CGTTATCAGG AACTGAAAGA CATCATCGCC + ATCCTGGGTA TGGATGAACT GTCTGAAGAA GACAAACTGG TGGTAGCGCG TGCTCGTAAG + ATCCAGCGCT TCCTGTCCCA GCCGTTCTTC GTGGCAGAAG TATTCACCGG TTCTCCGGGT + AAATACGTCT CCCTGAAAGA CACCATCCGT GGCTTTAAAG GCATCATGGA AGGCGAATAC + GATCACCTGC CGGAGCAGGC GTTCTACATG GTCGGTTCCA TCGAAGAAGC TGTGGAAAAA + GCCAAAAAAC TTTAACGCCT TAATCGGAGG GTGATATGGC AATGACTTAC CACCTGGACG + TCGTCAGCGC AGAGCAACAA ATGTTCTCTG GTCTGGTCGA GAAAATCCAG GTAACGGGTA + GCGAAGGTGA ACTGGGGATC TACCCTGGCC ACGCACCGCT GCTCACCGCC ATTAAGCCTG + GTATGATTCG CATCGTGAAA CAGCACGGTC ACGAAGAGTT TATCTATCTG TCTGGCGGCA + TTCTTGAAGT GCAGCCTGGC AACGTGACCG TTCTGGCCGA CACCGCAATT CGCGGCCAGG + ATCTCGACGA AGCGCGAGCC ATGGAAGCGA AACGTAAGGC TGAAGAGCAC ATTAGCAGCT + CTCACGGCGA CGTAGATTAC GCTCAGGCGT CTGCGGAACT GGCCAAAGCG ATCGCGCAGC + TGCGGTTATC GAGTTGACCA AAAAAGCGAT GTAACACCGG CTTGAAAAGC ACAAAAGCCA + GTCTGGAAAC AGGCTGGCTT TTTTTTGCGC GTGTGACCCG TCCTGAATAG CGTTCACATA + GATCCTGCTG ATATAAAACC CCCCTGTTTT CCTGTTTATT CATTGATCGA AATAAGAGCA + AAAACATCCA CCTGACGCTT AAATTAAGGT ACTGCCTTAA TTTTCTGCAG ACAAAAGGCG + TGACGATGGT CGAAAATGGC GCTTTCGTCA GCGGGGATAA TCCGTTATTG AACAATTTAT + CCTCTGTCCA TTTCACGATG AAAAAAATGT AGTTTTTTCA AGGTGAAGCG GTTTAAATTC + GTTCTCAAAT TACAGTCAGG ACGCGTATGT TGAATAATGC TATGAGCGTA GTGATCCTTG + CCGCACGAAG GCACGCGCAT GTATTCCGAT CTTCCGAAAG TGCTGCATAC CCTGCCGGGA + AAGCGATGGT TCAGCATGTC ATTGATGCTG CGAATGAATT AGGCGCACGG CACGTTCACC + TGGTGTACGG TCACGGCGGC GATCTGCTAA AACAGGCGCT GAAAGACGAC AACCTTAACT + GGGTGCTTCA GGCAGAGCAG CTGGGTACGG GTCATGCAAT GCAGCAGGCC GCACCTTTCT + TTGCCGATGA TGAAGACATT TTAATGCTCT ACGC +// diff --git a/tables/BAP.MRG b/tables/BAP.MRG new file mode 100644 index 0000000..aa2ec1a --- /dev/null +++ b/tables/BAP.MRG @@ -0,0 +1,11 @@ +PLOT POSITIONS FOR SAP. DRAWING BOARD SIZE, X0 XLENGTH, OPT Y0 YLEN + 10000 10000 + 1100 8500 + 12 5000 5000 + 13 1 100 + 15 1 10000 + 16 20 20 + 29 4000 1000 + 33 5000 4000 + 34 9000 1000 + 39 2000 2000 diff --git a/tables/BAP.MRG~ b/tables/BAP.MRG~ new file mode 100644 index 0000000..b9e90fd --- /dev/null +++ b/tables/BAP.MRG~ @@ -0,0 +1,10 @@ +PLOT POSITIONS FOR SAP. DRAWING BOARD SIZE, X0 XLENGTH, OPT Y0 YLEN + 10000 10000 + 1100 8500 + 12 5000 5000 + 13 1 100 + 15 1 10000 + 16 20 20 + 29 4000 1000 + 33 5000 4000 + 34 9000 1000 diff --git a/tables/DAP.MRG b/tables/DAP.MRG new file mode 100644 index 0000000..b9e90fd --- /dev/null +++ b/tables/DAP.MRG @@ -0,0 +1,10 @@ +PLOT POSITIONS FOR SAP. DRAWING BOARD SIZE, X0 XLENGTH, OPT Y0 YLEN + 10000 10000 + 1100 8500 + 12 5000 5000 + 13 1 100 + 15 1 10000 + 16 20 20 + 29 4000 1000 + 33 5000 4000 + 34 9000 1000 diff --git a/tables/INTRON.WTS b/tables/INTRON.WTS new file mode 100644 index 0000000..0c72d66 --- /dev/null +++ b/tables/INTRON.WTS @@ -0,0 +1,16 @@ + Mount donors redone 16-4-91 + 12 3 -16.085 -7.500 + P -2 -1 0 1 2 3 4 5 6 7 8 9 + N 136 136 136 136 136 136 136 136 136 136 136 136 + T 28 8 15 17 0 136 9 16 7 84 30 36 + C 41 60 16 7 0 0 3 13 3 17 28 39 + A 40 56 89 12 0 0 83 91 12 23 53 33 + G 27 12 16 100 136 0 41 16 114 12 25 28 + Mount acceptors redone 16-4-91 + 18 15 -26.142 -14.400 + P -14 -13 -12 -11 -10 -9 -8 -7 -6 -5 -4 -3 -2 -1 0 1 2 3 + N 113 113 113 113 113 113 113 113 113 113 113 113 113 113 113 113 113 113 + T 58 50 57 59 67 56 58 49 47 66 64 31 34 0 0 11 41 31 + C 21 28 34 25 29 33 35 32 42 40 33 25 74 0 0 23 28 41 + A 17 11 11 18 7 17 12 23 15 3 10 29 5 113 0 24 21 21 + G 17 24 11 11 10 7 8 9 9 4 6 28 0 0 113 55 23 20 diff --git a/tables/MEP.MRG b/tables/MEP.MRG new file mode 100644 index 0000000..98956a7 --- /dev/null +++ b/tables/MEP.MRG @@ -0,0 +1,9 @@ +PLOT POSITIONS FOR MEP. DRAWING BOARD SIZE, X0 XLENGTH, OPT Y0 YLEN + 10000 10000 + 1100 8500 + 12 20 9480 + 13 0 10000 + 15 20 9480 + 16 20 9480 + 17 500 9400 + 33 500 9400 diff --git a/tables/NIP.MRG b/tables/NIP.MRG new file mode 100644 index 0000000..1ae0315 --- /dev/null +++ b/tables/NIP.MRG @@ -0,0 +1,48 @@ +PLOT POSITIONS FOR ANALYSEQ. DRAWING BOARD SIZE, X0 XLENGTH, OPT Y0 YLEN + 10000 10000 + 1100 8500 + 12 20 9480 + 13 0 10000 + 15 20 9480 + 16 20 9480 + 17 500 9400 + 18 500 500 + 19 1000 500 + 20 1500 500 + 24 1500 500 + 25 2000 500 + 26 2500 500 + 27 3000 500 + 28 3500 500 + 29 4000 500 + 30 500 500 + 31 1000 2000 + 32 2000 2000 + 33 2000 500 + 34 2500 500 + 35 3000 500 + 36 3500 500 + 42 4000 5500 + 43 4000 5500 + 44 3000 1000 + 45 4000 5500 + 46 4000 5500 + 47 4000 5500 + 48 2000 1000 + 49 1000 500 + 50 4000 5500 + 51 4000 5500 + 52 4000 5500 + 53 4000 5500 + 55 1000 1500 + 56 6000 750 + 57 4500 1500 + 58 4000 5500 + 59 1000 500 + 60 1000 500 + 61 4000 5500 + 62 2000 1000 + 63 2000 1000 + 64 2000 1000 + 65 500 500 + 67 1000 1000 diff --git a/tables/NIPF.MRG b/tables/NIPF.MRG new file mode 100644 index 0000000..c968441 --- /dev/null +++ b/tables/NIPF.MRG @@ -0,0 +1,22 @@ +PLOT POSITIONS FOR nipf. DRAWING BOARD SIZE, X0 XLENGTH, OPT Y0 YLEN + 10000 10000 + 1100 8500 + 12 500 9500 + 13 0 10000 + 15 20 9480 + 16 20 9480 + 17 500 9400 + 18 500 500 + 19 1000 500 + 20 1500 500 + 24 1500 500 + 25 2000 1000 + 26 3000 1000 + 27 4000 1000 + 28 3500 500 + 29 4000 500 + 30 500 500 + 31 1000 1000 + 32 2000 2000 + 33 2000 500 + 34 2000 500 diff --git a/tables/NIPMARG b/tables/NIPMARG new file mode 100644 index 0000000..1ae0315 --- /dev/null +++ b/tables/NIPMARG @@ -0,0 +1,48 @@ +PLOT POSITIONS FOR ANALYSEQ. DRAWING BOARD SIZE, X0 XLENGTH, OPT Y0 YLEN + 10000 10000 + 1100 8500 + 12 20 9480 + 13 0 10000 + 15 20 9480 + 16 20 9480 + 17 500 9400 + 18 500 500 + 19 1000 500 + 20 1500 500 + 24 1500 500 + 25 2000 500 + 26 2500 500 + 27 3000 500 + 28 3500 500 + 29 4000 500 + 30 500 500 + 31 1000 2000 + 32 2000 2000 + 33 2000 500 + 34 2500 500 + 35 3000 500 + 36 3500 500 + 42 4000 5500 + 43 4000 5500 + 44 3000 1000 + 45 4000 5500 + 46 4000 5500 + 47 4000 5500 + 48 2000 1000 + 49 1000 500 + 50 4000 5500 + 51 4000 5500 + 52 4000 5500 + 53 4000 5500 + 55 1000 1500 + 56 6000 750 + 57 4500 1500 + 58 4000 5500 + 59 1000 500 + 60 1000 500 + 61 4000 5500 + 62 2000 1000 + 63 2000 1000 + 64 2000 1000 + 65 500 500 + 67 1000 1000 diff --git a/tables/PERCPTRON.WTS b/tables/PERCPTRON.WTS new file mode 100644 index 0000000..fd0d556 --- /dev/null +++ b/tables/PERCPTRON.WTS @@ -0,0 +1,56 @@ + + P-60-59-58-57-56-55-54-53-52-51-50-49-48-47-46-45-44-43-42-41-40-39-38-37-36 + T 5 1 -3 9-14 7 15 -5 3-16-17 4 18 5 -3 -1 2 4 5 -5 7 8 -5-15 6 + C-21 -6-11-21 0 8 -7-12 -1 1 0-19 12 -3 -1 10 2 -8 -5-11 8 1 23 6 -5 + A 7 -2 13 -2 -8-13-18 5 0 -5 13 8-15 9 -4 -7 9 0 -8-11-10 -6 -7 -5 -6 + G -6 -9 -7 0 8-16 -4 -2-16 1 -4 8-14 5 11-13-24 3 7 22-11 -9-15 10 -4 + + P-35-34-33-32-31-30-29-28-27-26-25-24-23-22-21-20-19-18-17-16-15-14-13-12-11 + T 3 4 16 -4 7 11 -4 -1 12 8 10 -1 1 8 2-10-16 11 1 -3 16 -3-36 -8-27 + C 2-14 -3 -8-10-21 2 0 -2 -1-11 -3 -1 5-11 -4 7 0-14 6 -8-20 -7-36-44 + A-12 -1-27 -3 -6 0-12 -3 -4 -7 14 -2 -4 -6 0 12 5 -9 0-11-11 10 8 2 8 + G 4 -5 -6 -3 -1 -4 -1 -4-15 0-14 3 10-19 -3-10 -7 -7 7 1 -8 -6 15 21 42 + + P-10 -9 -8 -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 + T-53-27-26-23 2 -7-14-40-28 0-53 75-62-20-40-10-35 -5-12 -1 4 14-23 7 -2 + C-15-50-43-35-38-29-29 1 -9 1-87-55-64-45 11-22-14-20-15-15-10-22 -5 2 6 + A 0 -3 -5 4-20-11 5 6 -2-15 66-69-52 -5 -4 6 8-24 -7-10 -7 13 14 -9-18 + G 35 22 16 -6 -5-15-25-33-28-53-36-50107 -5-37-44-27-15-23-16-29-47-17-29-15 + + P 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 + T-26 1 4 -7 3 -4 0-10 8-18 7-22-21 8 4 -3 -6 7 -8 1 -5-16-16 7 -6 + C 6 -8 19 -7 9 -3 17 -2 3 -9 5 22 22 8 -1 1 18 6 11-10 -8 7 10 0 7 + A 14-12-42 1 -5 -4-32 12-10 20 -6 -1 3 -4 4-10 -1 -2-14 11 14 -3 2-13 5 + G-23 -7 -1 -6-17 -4 0-15-14 -4-17-10 -5-13 -8 10-13-13 9 -4 -3 10 2 4 -8 + + P 40 + T 0 + C 14 + A 5 + G-21 + +These come from w101 of Stormo, Schneider, Gold and Ehrenfeucht nar 10 2997- +3011, 1982. They report that this matrix gives a score of at least 2 for all +gene starts in their library whereas all other sequences score 1 or less. The +weightings were derived using the perceptron algorithm in the following way: +want to find a weighting function w such that w.s > t if and only if s is a +ribosome binding site; divide the sequences into s+ (gene starts) and s- (other +sequences in the libarary); perform the following procedure: + + test: choose a sequence s from s+ or s- + if s is in s+ and w.s >= t go to test + if s is in s+ and w.s <= t go to add + if s is in s- and w.s < t go to test + if s is in s- and w.s >= t go to subtract + add: replace w by w + s + go to test +subtract: replace w by w - s + go to test + +this is repeated until differences between w in successive rounds are very +small. +Shorter lengths for w were found to be less good although protection +experiments show that the ribosome interacts with at most 35-40 bases. +Note AUG, GUG and UUG can act as initiation codons; AUA will initiate at +low rates in vivo and essentially any RNA polymer can be made to initiate +translation under appropriate ionic conditions in vitro. diff --git a/tables/PIP.MRG b/tables/PIP.MRG new file mode 100644 index 0000000..681c3b4 --- /dev/null +++ b/tables/PIP.MRG @@ -0,0 +1,18 @@ +PLOT POSITIONS FOR ANALYSEP. DRAWING BOARD SIZE, X0 XLENGTH, OPT Y0 YLEN + 10000 10000 + 1100 8500 + 12 20 9480 + 13 0 10000 + 15 20 9480 + 16 20 9480 + 17 20 9480 + 18 500 500 + 19 1000 500 + 20 1500 500 + 22 1000 1000 + 23 2000 1000 + 24 3000 6500 + 25 1000 4000 + 26 100 9800 + 27 1000 1000 + 28 1000 1000 diff --git a/tables/PROKPROMS.WTS b/tables/PROKPROMS.WTS new file mode 100644 index 0000000..5f939f1 --- /dev/null +++ b/tables/PROKPROMS.WTS @@ -0,0 +1,52 @@ +prokaryotic wts for promoters +-35 region: +P -50-49-48-47-46-45-44-43-42-41-40-39-38-37-36-35-34-33-32-31-30-29-28-27-26 + 107109109110110110110110110111111110111112112112112112112112112112112112112 +T 41 33 32 25 34 22 35 35 42 27 32 42 47 14 92 94 11 19 15 37 46 34 38 48 34 +C 22 27 18 29 20 14 20 12 22 23 16 25 10 43 7 6 11 18 60 8 25 23 23 17 20 +A 28 38 30 37 35 56 42 42 37 42 39 18 25 26 2 6 2 72 26 50 26 34 25 26 31 +G 16 11 29 19 21 18 13 21 9 19 24 26 29 29 11 6 88 3 11 17 15 21 26 21 27 +-10 region: +P -23-22-21-20-19-18-17-16-15-14-13-12-11-10 -9 -8 -7 -6 -5 + 112112112112112112112112112112112112112112112112112112112 +T 35 28 28 27 39 51 34 43 26 31 89 3 49 15 19108 31 29 21 +C 34 21 24 27 12 25 20 25 20 27 10 2 16 14 22 3 13 16 30 +A 20 39 33 33 39 23 29 16 23 19 2106 29 66 57 1 35 23 31 +G 23 24 27 25 22 13 29 28 43 35 11 1 18 17 14 0 33 24 30 ++ region: +P -2 -1 1 2 3 4 5 6 7 8 9 10 + 86 88 88 88 88 88 88 88 88 88 88 88 +T 16 22 2 42 27 23 20 25 27 15 16 29 +C 29 49 4 25 25 13 18 22 17 17 16 17 +A 20 9 45 16 24 25 28 24 24 32 35 26 +G 21 8 37 5 12 27 22 17 20 24 21 16 +Notes: +D. K. and McClure, R., nar 11 2237-2255 (1983) + +E. coli promoters have been shown to contain 2 regions of conserved sequence +located about 10 and 35 bases upstream of the transcription startsite. These +are TATAAT and TTGACA with an allowed spacing of 15 to 21 bases between. The +spacing with maximum efficiency was 17 bases and all but 12 of the 112 +sequences could be aligned with a separation of 17 +or-1 bases. The standard +promoter has spacing 7 and 17 bases between the startsite and the -10 region, +and the -10 and -35 regions, respectively. The spacing between the -10 region +and the startsite is usually 6 or 7 bases but varies between 4 and 8 bases. +There is an AT rich region of 8 to 10 bases upstream of the -35 region. +Iniation with a purine is highly prefered with G being used if A is not +present. +Gap penalties: + 15 0.02 (only exists as mutant) + 16 0.2 + 17 1.0 + 18 0.2 + 19 0.05 (guess) + 20 0.02 (guess) + 21 0.01 (guess) +Two processes in volved: 1) recognition + 2) melting (needs recognition for initiation + and then AT richness) + +factor of about 1 order of magnitude difference for all AT against all GC +in terms of activity. +Andrew Travers says there are upstream regions that resemble -35 and -10 +regions (on both strands). diff --git a/tables/PROTEIN.ALL b/tables/PROTEIN.ALL new file mode 100644 index 0000000..7bd2cf3 --- /dev/null +++ b/tables/PROTEIN.ALL @@ -0,0 +1,21 @@ +A/A// +C/C// +D/D// +E/E// +F/F// +G/G// +H/H// +I/I// +K/K// +L/L// +M/M// +N/N// +P/P// +Q/Q// +R/R// +S/S// +T/T// +V/V// +W/W// +Y/Y// + diff --git a/tables/PROTEIN.GRP b/tables/PROTEIN.GRP new file mode 100644 index 0000000..ef2b834 --- /dev/null +++ b/tables/PROTEIN.GRP @@ -0,0 +1,10 @@ +ACID/D/E// +BASIC/R/K/H// +HYDRO/F/L/I/V/Y// +GLYCO/N-S/N-T// ++/R/K/H// +-/D/E// +DIBASIC/RR/KK/RK/KR// +TURN/N/D/G/P/S// +BLOCK/A/Q/E/I/L/M/F/W/V// +INDIF/R/C/H/K/T/Y// diff --git a/tables/PROTMAT.DAT b/tables/PROTMAT.DAT new file mode 100644 index 0000000..138f617 --- /dev/null +++ b/tables/PROTMAT.DAT @@ -0,0 +1,29 @@ + C S T P A G N D E Q B Z H R K M I L V F Y W - X ? +C 22 10 8 7 8 7 6 5 5 5 5 5 7 6 5 5 8 4 8 6 10 2 10 10 10 10 +S 10 12 11 11 11 11 11 10 10 9 10 10 9 10 10 8 9 7 9 7 7 8 10 10 10 10 +T 8 11 13 10 11 10 10 10 10 9 10 10 9 9 10 9 10 8 10 7 7 5 10 10 10 10 +P 7 11 10 16 11 9 9 9 9 10 9 10 10 10 9 8 8 7 9 5 5 4 10 10 10 10 +A 8 11 11 11 12 11 10 10 10 10 10 10 9 8 9 9 9 8 10 6 7 4 10 10 10 10 +G 7 11 10 9 11 15 10 11 10 9 10 10 8 7 8 7 7 6 9 5 5 3 10 10 10 10 +N 6 11 10 9 10 10 12 12 11 11 12 11 12 10 11 8 8 7 8 6 8 6 10 10 10 10 +D 5 10 10 9 10 11 12 14 13 12 13 12 11 9 10 7 8 6 8 4 6 3 10 10 10 10 +E 5 10 10 9 10 10 11 13 14 12 12 13 11 9 10 8 8 7 8 5 6 3 10 10 10 10 +Q 5 9 9 10 10 9 11 12 12 14 11 13 13 11 11 9 8 8 8 5 6 5 10 10 10 10 +B 5 10 10 9 10 10 12 13 12 11 13 11 11 10 10 8 8 6 8 5 7 4 10 10 10 10 +Z 5 10 10 10 10 10 11 12 13 13 11 14 12 10 10 8 8 8 8 5 6 4 10 10 10 10 +H 7 9 9 10 9 8 12 11 11 13 11 12 16 12 10 8 8 8 8 8 10 7 10 10 10 10 +R 6 10 9 10 8 7 10 9 9 11 10 10 12 16 13 10 8 7 8 6 6 12 10 10 10 10 +K 5 10 10 9 9 8 11 10 10 11 10 10 10 13 15 10 8 7 8 5 6 7 10 10 10 10 +M 5 8 9 8 9 7 8 7 8 9 8 8 8 10 10 16 12 14 12 10 8 6 10 10 10 10 +I 8 9 10 8 9 7 8 8 8 8 8 8 8 8 8 12 15 12 14 11 9 5 10 10 10 10 +L 4 7 8 7 8 6 7 6 7 8 6 8 8 7 7 14 12 16 12 12 9 8 10 10 10 10 +V 8 9 10 9 10 9 8 8 8 8 8 8 8 8 8 12 14 12 14 9 8 4 10 10 10 10 +F 6 7 7 5 6 5 6 4 5 5 5 5 8 6 5 10 11 12 9 19 17 10 10 10 10 10 +Y 10 7 7 5 7 5 8 6 6 6 7 6 10 6 6 8 9 9 8 17 20 10 10 10 10 10 +W 2 8 5 4 4 3 6 3 3 5 4 4 7 12 7 6 5 8 4 10 10 27 10 10 10 10 +- 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 +X 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 +? 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 + 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 + + diff --git a/tables/README b/tables/README new file mode 100644 index 0000000..0da80c0 --- /dev/null +++ b/tables/README @@ -0,0 +1,50 @@ +Sequence Library setup files + + +Default files (What we currently use) +------------------------------------- +EMBLLIBDESCRP +EMBLdiv.lkp +GENBLIBDESCRP +GENBdiv.lkp +NRL3DLIBDESCRP +NRL3Ddiv.lkp +PIRLIBDESCRP +PIRdiv.lkp +SEQUENCELIBRARIES +SWISSLIBDESCRP +SWISSdiv.lkp + + +Sun mounted EMBL CDROM +---------------------- +EMBLLIBDESCRP-SUN +EMBLdiv.lkp-sun +SEQUENCELIBRARIES-SUN +SWISSLIBDESCRP-SUN +SWISSdiv.lkp-sun + + +IBM AIX, DEC Ultrix mounted EMBL CDROM +-------------------------------------- +EMBLLIBDESCRP-DEC +EMBLdiv.lkp-dec +SEQUENCELIBRARIES-DEC +SWISSLIBDESCRP-DEC +SWISSdiv.lkp-dec + +Generic files (suitable for templates) +-------------------------------------- +EMBLLIBDESCRP-GENERIC +EMBLdiv.lkp-generic +GENBLIBDESCRP-GENERIC +GENBdiv.lkp-generic +NRL3DLIBDESCRP-GENERIC +NRL3Ddiv.lkp-generic +PIRLIBDESCRP-GENERIC +PIRdiv.lkp-generic +SEQUENCELIBRARIES-GENERIC +SWISSLIBDESCRP-GENERIC +SWISSdiv.lkp-generic + + diff --git a/tables/RENZYM.4 b/tables/RENZYM.4 new file mode 100644 index 0000000..27570cc --- /dev/null +++ b/tables/RENZYM.4 @@ -0,0 +1,22 @@ +ALUI/AG'CT// +CFOI/GCG'C// +DDEI/C'TNAG// +FNU4HI/GC'NGC// +FNUDII/CG'CG// +HAEIII/GG'CC// +HINFI/G'ANTC// +HINPI/G'CGC// +HPAII/C'CGG// +MAEI/C'TAG// +MAEII/A'CGT// +MAEIII/N'GTNAC// +MBOI/'GATC// +MNLI/CCTCNNNNNNN'/'NNNNNNNGAGG// +NLAIII/CATG'// +NLAIV/GGN'NCC// +RSAI/GT'AC// +SAU96I/G'GNCC// +SCRFI/CC'NGG// +SECI/C'CNNGG// +SFII/GGCCNNNN'NGGCC// +TAQI/T'CGA// diff --git a/tables/RENZYM.6 b/tables/RENZYM.6 new file mode 100644 index 0000000..a54f191 --- /dev/null +++ b/tables/RENZYM.6 @@ -0,0 +1,85 @@ +AATII/GACGT'C// +ACCI/GT'MKAC// +AFLII/C'TTAAG// +AFLIII/A'CRYGT// +AHAII/GR'CGYC// +APAI/GGGCC'C// +APALI/G'TGCAC// +ASUII/TT'CGAA// +AVAI/C'YCGRG// +AVAII/G'GWCC// +AVRII/C'CTAGG// +BALI/TGG'CCA// +BAMHI/G'GATCC// +BANI/G'GYRCC// +BANII/GRGCY'C// +BBVI/GCAGCNNNNNNNN'/'NNNNNNNNNNNNGCTGC// +BCLI/T'GATCA// +BGLI/GCCNNNN'NGGC// +BGLII/A'GATCT// +BINI/GGATCNNNN'/'NNNNNGATCC// +BSMI/GAATGCN'/NG'CATTC// +BSP1286/GDGCH'C// +BSPMI/ACCTGCNNNN'/'NNNNNNNNGCAGGT// +BSPMII/TCCGG'A// +BSSHII/G'CGCGC// +BSTEII/G'GTNACC// +BSTXI/CCANNNNN'NTGG// +BSTNI/CC'WGG// +CLAI/AT'CGAT// +DRAI/TTT'AAA// +DRAII/RG'GNCCY// +DRAIII/CACNNN'GTG// +EAEI/Y'GGCCR// +ECOB/TGANNNNNNNNTGCT/AGCANNNNNNNNTCA// +ECOK/AACNNNNNNGTGC/GCACNNNNNNGTT// +ECORI/G'AATTC// +ECORV/GAT'ATC// +ESPI/GC'TNAGC// +FOKI/GGATGNNNNNNNNN'/'NNNNNNNNNNNNNCATCC// +FSPI/TGC'GCA// +HAEII/RGCGC'Y// +HGAI/GACGCNNNNN'/'NNNNNNNNNNGCGTC// +HGIAI/GWGCW'C// +HINCII/GTY'RAC// +HINDIII/A'AGCTT// +HPAI/GTT'AAC// +HPHI/GGTGANNNNNNNN'/'NNNNNNNTCACC// +KPNI/GGTAC'C// +MBOII/GAAGANNNNNNNN'/'NNNNNNNTCTTC// +MLUI/A'CGCGT// +MSTII/CC'TNAGG// +NAEI/GCC'GGC// +NARI/GG'CGCC// +NCII/CC'SGG// +NCOI/C'CATGG// +NDEI/CA'TATG// +NHEI/G'CTAGC// +NOTI/GC'GGCCGC// +NRUI/TCG'CGA// +NSII/ATGCA'T// +NSPBII/CMG'CKG// +PFIMI/CCANNNN'NTGG// +PPUMI/RG'GWCCY// +PSTI/CTGCA'G// +PVUI/CGAT'CG// +PVUII/CAG'CTG// +RSRII/CG'GWCCG// +SACI/GAGCT'C// +SACII/CCGC'GG// +SALI/G'TCGAC// +SCAI/AGT'ACT// +SFANI/GATGCNNNNN'/'NNNNNNNNNGCATC// +SMAI/CCC'GGG// +SNABI/TAC'GTA// +SPEI/A'CTAGT// +SPHI/GCATG'C// +SSPI/AAT'ATT// +STUI/AGG'CCT// +STYI/C'CWWGG// +TTHIIII/GACN'NNGTC// +XBAI/T'CTAGA// +XHOI/C'TCGAG// +XHOII/R'GATCY// +XMAIII/C'GGCCG// +XMNI/GAANN'NNTTC// diff --git a/tables/RENZYM.ALL b/tables/RENZYM.ALL new file mode 100644 index 0000000..bf78e7d --- /dev/null +++ b/tables/RENZYM.ALL @@ -0,0 +1,316 @@ +; REBASE 9210 from Rich Roberts +AatI/AGG'CCT// +AatII/GACGT'C// +Acc65I/G'GTACC// +AccI/GT'MKAC// +AccII/CG'CG// +AccIII/T'CCGGA// +AciI/C'CGC/G'CGG// +AcyI/GR'CGYC// +AfaI/GT'AC// +AflII/C'TTAAG// +AflIII/A'CRYGT// +AgeI/A'CCGGT// +AluI/AG'CT// +Alw21I/GWGCW'C// +Alw26I/GTCTCN'NNNN/'NNNNNGAGAC// +Alw44I/G'TGCAC// +AlwI/GGATCNNNN'N/'NNNNNGATCC// +AlwNI/CAGNNN'CTG// +Aor51HI/AGC'GCT// +ApaI/GGGCC'C// +ApaLI/G'TGCAC// +ApoI/R'AATTY// +ApyI/CC'WGG// +AscI/GG'CGCGCC// +AseI/AT'TAAT// +AsnI/AT'TAAT// +Asp700I/GAANN'NNTTC// +Asp718I/G'GTACC// +AspEI/GACNNN'NNGTC/GACNN'NNNGTC// +AspHI/GWGCW'C// +AspI/GACN'NNGTC// +AsuI/G'GNCC// +AvaI/C'YCGRG// +AvaII/G'GWCC// +AviII/TGC'GCA// +AvrII/C'CTAGG// +AxyI/CC'TNAGG// +BalI/TGG'CCA// +BamHI/G'GATCC// +BanI/G'GYRCC// +BanII/GRGCY'C// +BanIII/AT'CGAT// +BbeI/GGCGC'C// +BbiII/GR'CGYC// +BbrPI/CAC'GTG// +BbsI/GAAGACNN'NNNN/'NNNNNNGTCTTC// +BbuI/GCATG'C// +BbvI/GCAGCNNNNNNNN'NNNN/'NNNNNNNNNNNNGCTGC// +BcgI/GCANNNNNNTCGNNNNNNNNNNNN'/NN'NNNNNNNNNNCGANNNNNNTGC// +BclI/T'GATCA// +BcnI/CC'SGG// +BcoI/C'YCGRG// +BfaI/C'TAG// +BfrI/C'TTAAG// +BglI/GCCNNNN'NGGC// +BglII/A'GATCT// +BlnI/C'CTAGG// +BmyI/GDGCH'C// +BpmI/CTGGAGNNNNNNNNNNNNNNNN'/NN'NNNNNNNNNNNNNNCTCCAG// +Bpu1102I/GC'TNAGC// +BsaAI/YAC'GTR// +BsaBI/GATNN'NNATC// +BsaHI/GR'CGYC// +BsaI/GGTCTCN'NNNN/'NNNNNGAGACC// +BsaJI/C'CNNGG// +BsaWI/W'CCGGW// +BscBI/GGN'NCC// +BscCI/GAATGCN'/NG'CATTC// +BscI/AT'CGAT// +BseAI/T'CCGGA// +BseNI/ACTGGN'/NC'CAGT// +BsgI/GTGCAGNNNNNNNNNNNNNNNN'/NN'NNNNNNNNNNNNNNCTGCAC// +Bsh1236I/CG'CG// +Bsh1285I/CGRY'CG// +BshI/GG'CC// +BsiBI/GATNN'NNATC// +BsiCI/TT'CGAA// +BsiEI/CGRY'CG// +BsiHKAI/GWGCW'C// +BsiLI/CC'WGG// +BsiMI/T'CCGGA// +BsiQI/T'GATCA// +BsiWI/C'GTACG// +BsiXI/AT'CGAT// +BsiYI/CCNNNNN'NNGG// +BsiZI/G'GNCC// +BslI/CCNNNNN'NNGG// +BsmAI/GTCTCN'NNNN/'NNNNNGAGAC// +BsmI/GAATGCN'/NG'CATTC// +Bsp106I/AT'CGAT// +Bsp119I/TT'CGAA// +Bsp120I/G'GGCCC// +Bsp1286I/GDGCH'C// +Bsp1407I/T'GTACA// +Bsp143I/'GATC// +Bsp143II/RGCGC'Y// +Bsp50I/CG'CG// +Bsp68I/TCG'CGA// +BspCI/CGAT'CG// +BspDI/AT'CGAT// +BspEI/T'CCGGA// +BspHI/T'CATGA// +BspMI/ACCTGCNNNN'NNNN/'NNNNNNNNGCAGGT// +BspWI/GCNNNNN'NNGC// +BspXI/AT'CGAT// +BsrBI/GAG'CGG/CCG'CTC// +BsrFI/R'CCGGY// +BsrI/ACTGGN'/NC'CAGT// +BssHII/G'CGCGC// +Bst1107I/GTA'TAC// +Bst71I/GCAGCNNNNNNNN'NNNN/'NNNNNNNNNNNNGCTGC// +BstBI/TT'CGAA// +BstEII/G'GTNACC// +BstI/G'GATCC// +BstNI/CC'WGG// +BstOI/CC'WGG// +BstPI/G'GTNACC// +BstUI/CG'CG// +BstXI/CCANNNNN'NTGG// +BstYI/R'GATCY// +BstZI/C'GGCCG// +Bsu15I/AT'CGAT// +Bsu36I/CC'TNAGG// +BsuRI/GG'CC// +CcrI/C'TCGAG// +CelII/GC'TNAGC// +CfoI/GCG'C// +Cfr10I/R'CCGGY// +Cfr13I/G'GNCC// +Cfr42I/CCGC'GG// +Cfr9I/C'CCGGG// +ClaI/AT'CGAT// +CpoI/CG'GWCCG// +Csp45I/TT'CGAA// +Csp6I/G'TAC// +CspI/CG'GWCCG// +CvnI/CC'TNAGG// +DdeI/C'TNAG// +DpnI/GA'TC// +DpnII/'GATC// +DraI/TTT'AAA// +DraII/RG'GNCCY// +DraIII/CACNNN'GTG// +DrdI/GACNNNN'NNGTC// +DsaI/C'CRYGG// +DsaV/'CCNGG// +EaeI/Y'GGCCR// +EagI/C'GGCCG// +Eam1104I/CTCTTCN'NNN/'NNNNGAAGAG// +Eam1105I/GACNNN'NNGTC// +EarI/CTCTTCN'NNN/'NNNNGAAGAG// +Ecl136II/GAG'CTC// +EclXI/C'GGCCG// +Eco105I/TAC'GTA// +Eco130I/C'CWWGG// +Eco147I/AGG'CCT// +Eco24I/GRGCY'C// +Eco31I/GGTCTCN'NNNN/'NNNNNGAGACC// +Eco32I/GAT'ATC// +Eco47I/G'GWCC// +Eco47III/AGC'GCT// +Eco52I/C'GGCCG// +Eco57I/CTGAAGNNNNNNNNNNNNNNNN'/NN'NNNNNNNNNNNNNNCTTCAG// +Eco64I/G'GYRCC// +Eco72I/CAC'GTG// +Eco81I/CC'TNAGG// +Eco88I/C'YCGRG// +Eco91I/G'GTNACC// +EcoNI/CCTNN'NNNAGG// +EcoO109I/RG'GNCCY// +EcoO65I/G'GTNACC// +EcoRI/G'AATTC// +EcoRII/'CCWGG// +EcoRV/GAT'ATC// +EcoT14I/C'CWWGG// +EcoT22I/ATGCA'T// +EheI/GGC'GCC// +Esp3I/CGTCTCN'NNNN/'NNNNNGAGACG// +EspI/GC'TNAGC// +FbaI/T'GATCA// +FdiII/TGC'GCA// +Fnu4HI/GC'NGC// +FokI/GGATGNNNNNNNNN'NNNN/'NNNNNNNNNNNNNCATCC// +FspI/TGC'GCA// +GsuI/CTGGAGNNNNNNNNNNNNNNNN'/NN'NNNNNNNNNNNNNNCTCCAG// +HaeII/RGCGC'Y// +HaeIII/GG'CC// +HapII/C'CGG// +HgaI/GACGCNNNNN'NNNNN/'NNNNNNNNNNGCGTC// +HgiAI/GWGCW'C// +HhaI/GCG'C// +Hin1I/GR'CGYC// +Hin6I/G'CGC// +HincII/GTY'RAC// +HindII/GTY'RAC// +HindIII/A'AGCTT// +HinfI/G'ANTC// +HinP1I/G'CGC// +HpaI/GTT'AAC// +HpaII/C'CGG// +HphI/GGTGANNNNNNNN'/N'NNNNNNNTCACC// +KasI/G'GCGCC// +Kpn2I/T'CCGGA// +KpnI/GGTAC'C// +Ksp632I/CTCTTCN'NNN/'NNNNGAAGAG// +KspI/CCGC'GG// +LspI/TT'CGAA// +MaeI/C'TAG// +MaeII/A'CGT// +MaeIII/'GTNAC// +MamI/GATNN'NNATC// +MboI/'GATC// +MboII/GAAGANNNNNNNN'/N'NNNNNNNTCTTC// +McrI/CGRY'CG// +MflI/R'GATCY// +MluI/A'CGCGT// +MnlI/CCTCNNNNNNN'/N'NNNNNNGAGG// +Mph1103I/ATGCA'T// +MroI/T'CCGGA// +MscI/TGG'CCA// +MseI/T'TAA// +MspI/C'CGG// +MstI/TGC'GCA// +MstII/CC'TNAGG// +MunI/C'AATTG// +MvaI/CC'WGG// +MvnI/CG'CG// +NaeI/GCC'GGC// +NarI/GG'CGCC// +NciI/CC'SGG// +NcoI/C'CATGG// +NdeI/CA'TATG// +NdeII/'GATC// +NgoMI/G'CCGGC// +NheI/G'CTAGC// +NlaIII/CATG'// +NlaIV/GGN'NCC// +NotI/GC'GGCCGC// +NruI/TCG'CGA// +NsiI/ATGCA'T// +NspBII/CMG'CKG// +NspI/RCATG'Y// +NspIII/C'YCGRG// +NspV/TT'CGAA// +PacI/TTAAT'TAA// +PaeI/GCATG'C// +PaeR7I/C'TCGAG// +PalI/GG'CC// +Pfl23II/C'GTACG// +PflMI/CCANNNN'NTGG// +PleI/GAGTCNNNN'N/'NNNNNGACTC// +PmaCI/CAC'GTG// +PmeI/GTTT'AAAC// +PmlI/CAC'GTG// +Ppu10I/A'TGCAT// +PpuMI/RG'GWCCY// +PshAI/GACNN'NNGTC// +Psp1406I/AA'CGTT// +Psp5II/RG'GWCCY// +PspAI/C'CCGGG// +PssI/RGGNC'CY// +PstI/CTGCA'G// +PvuI/CGAT'CG// +PvuII/CAG'CTG// +RmaI/C'TAG// +RsaI/GT'AC// +RsrII/CG'GWCCG// +SacI/GAGCT'C// +SacII/CCGC'GG// +SalI/G'TCGAC// +SapI/GCTCTTCN'NNN/'NNNNGAAGAGC// +Sau3AI/'GATC// +Sau96I/G'GNCC// +SauI/CC'TNAGG// +ScaI/AGT'ACT// +ScrFI/CC'NGG// +SduI/GDGCH'C// +SfaNI/GCATCNNNNN'NNNN/'NNNNNNNNNGATGC// +SfcI/C'TRYAG// +SfiI/GGCCNNNN'NGGCC// +SfuI/TT'CGAA// +SgrAI/CR'CCGGYG// +SinI/G'GWCC// +SmaI/CCC'GGG// +SnaBI/TAC'GTA// +SnoI/G'TGCAC// +SpeI/A'CTAGT// +SphI/GCATG'C// +SplI/C'GTACG// +SpoI/TCG'CGA// +SrfI/GCCC'GGGC// +Sse8387I/CCTGCA'GG// +SspI/AAT'ATT// +SstI/GAGCT'C// +SstII/CCGC'GG// +StuI/AGG'CCT// +StyI/C'CWWGG// +SunI/C'GTACG// +SwaI/ATTT'AAAT// +TaqI/T'CGA// +TfiI/G'AWTC// +ThaI/CG'CG// +Tth111I/GACN'NNGTC// +TthHB8I/T'CGA// +Van91I/CCANNNN'NTGG// +VspI/AT'TAAT// +XbaI/T'CTAGA// +XcmI/CCANNNNN'NNNNTGG// +XhoI/C'TCGAG// +XhoII/R'GATCY// +XmaI/C'CCGGG// +XmaIII/C'GGCCG// +XmnI/GAANN'NNTTC// +XorII/CGAT'CG// + diff --git a/tables/RIBSEUKAR.WTS b/tables/RIBSEUKAR.WTS new file mode 100644 index 0000000..71ee71f --- /dev/null +++ b/tables/RIBSEUKAR.WTS @@ -0,0 +1,22 @@ +mRNA WTS FOR EUKARYOTES SARGAN,GREGORY,BUTTERWORTH FEBS LET 147 133-136 1982 +P -7 -6 -5 -4 -3 -2 -1 1 2 3 + 102102102102102102102102102102 +T 19 24 31 12 0 18 5 0102 0 +C 20 15 32 65 5 42 52 0 0 0 +A 50 27 27 19 86 36 34102 0 0 +G 6 29 12 6 11 6 11 0 0102 +VIRAL ONLY +P -7 -6 -5 -4 -3 -2 -1 1 2 3 + 41 41 41 41 41 41 41 41 41 41 +T 14 12 16 4 2 13 9 0 41 0 +C 7 3 13 17 7 9 14 0 0 0 +A 15 10 6 10 27 15 9 41 0 0 +G 5 16 6 10 5 4 9 0 0 41 +This paper puts forward the hypothesis that there is an interaction between +some mRNA leader sequences and a highly conserved structure in the 18S rRNA +of eukaryotic ribosomes. The attempt to substantiate the hypothesis includes +a table of base frequencies for sequences immediately 5' to start codons. +They examined 102 sequences and I have used the base frequencies they found +as a weight matrix for searching for eukaryotic gene starts. I don't yet +know how good this method is. The viral sequences were found to be slightly +different but the separate table shown here is not used in the program. diff --git a/tables/ROBSON.WTS b/tables/ROBSON.WTS new file mode 100644 index 0000000..ed14311 --- /dev/null +++ b/tables/ROBSON.WTS @@ -0,0 +1,112 @@ + DIRECTIONAL INFORMATION MEASURE FOR THE ALPHA-HELICAL CONFORMATION + J-8 J-7 J-6 J-5 J-4 J-3 J-2 J-1 J J+1 J+2 J+3 J+4 J+5 J+6 J+7 J+8 + C 0 0 0 0 0 0 -5 -10 -13 -10 -5 0 0 0 0 0 0 + S 0 -5 -10 -15 -20 -25 -30 -35 -39 -35 -30 -25 -20 -15 -10 -5 0 + T 0 0 0 -5 -10 -15 -20 -25 -26 -25 -20 -15 -10 -5 0 0 0 + P -10 -20 -40 -60 -80-100-120-140 -77 -60 -30 -20 -10 0 0 0 0 + A 5 10 15 20 30 40 50 60 65 60 50 40 30 20 15 10 5 + G -5 -10 -15 -20 -30 -40 -50 -60 -86 -60 -50 -40 -30 -20 -15 -10 -5 + N 0 0 0 0 -10 -20 -30 -40 -51 -40 -30 -20 -10 0 0 0 0 + D 0 -5 -10 -15 -20 -15 -10 0 5 10 15 20 20 20 15 10 5 + E 0 0 0 0 10 20 60 70 78 78 78 78 78 70 60 40 20 + Q 0 0 0 0 5 10 20 20 10 -10 -20 -20 -10 -5 0 0 0 + B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + Z 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + H 10 20 30 40 50 50 50 30 12 -20 -10 0 0 0 0 0 0 + R 0 0 0 0 0 0 0 0 -9 -15 -20 -30 -40 -50 -50 -30 -10 + K 20 40 50 55 60 60 50 30 23 10 5 0 0 0 0 0 0 + M 10 20 25 30 35 40 45 50 53 50 45 40 35 30 25 20 10 + I 5 10 15 20 25 20 15 10 6 0 -10 -15 -20 -25 -20 -10 -5 + L 0 5 10 15 20 25 28 30 32 30 28 25 20 15 10 5 0 + V 0 0 0 0 0 0 5 10 14 10 5 0 0 0 0 0 0 + F 0 0 0 0 0 5 10 15 16 15 10 5 0 0 0 0 0 + Y -5 -10 -15 -20 -25 -30 -35 -40 -45 -40 -35 -30 -25 -20 -15 -10 -5 + W -10 -20 -40 -50 -50 -10 0 10 12 10 0 -10 -50 -50 -40 -20 -10 + - 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + X 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + ? 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + DIRECTIONAL INFORMATION MEASURE FOR THE EXTENDED CONFORMATION + J-8 J-7 J-6 J-5 J-4 J-3 J-2 J-1 J J+1 J+2 J+3 J+4 J+5 J+6 J+7 J+8 + C 0 0 0 0 0 10 20 30 44 30 20 10 0 0 0 0 0 + S 0 10 20 10 0 -5 -10 -15 -17 -15 -10 -5 0 10 20 10 0 + T 5 10 15 20 15 15 10 10 13 10 10 15 15 20 15 10 5 + P 10 20 30 30 20 10 0 -10 -18 -20 -10 10 30 40 30 20 10 + A 0 0 0 0 -5 -10 -15 -20 -23 -20 -15 -10 -5 0 0 0 0 + G 10 20 30 40 40 20 0 -20 -42 -20 0 20 40 40 30 20 -10 + N 10 30 50 30 20 0 -15 -30 -41 -30 -15 0 20 30 50 30 10 + D 0 5 10 15 20 0 -20 -30 -44 -30 -20 0 0 0 0 0 0 + E -10 -15 -20 -25 -30 -35 -40 -45 -50 -55 -60 -60 -50 -40 -30 -20 -10 + Q 0 0 0 0 0 -5 -10 0 12 20 30 40 50 50 40 30 15 + B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + Z 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + H -10 -20 -40 -20 -10 0 -10 -20 -25 -35 -30 -25 -20 -15 -10 -5 0 + R 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 + K -5 -10 -15 -20 -30 -40 -50 -40 -33 -20 -10 0 10 10 0 0 0 + M -10 -20 -30 -40 -40 -30 0 10 23 10 0 -30 -40 -40 -30 -20 -10 + I 0 -10 -20 -10 0 20 40 60 67 60 40 20 0 -10 -20 -10 0 + L 0 0 0 0 0 5 10 20 23 20 10 5 0 0 0 0 0 + V 0 0 -10 -20 0 20 40 60 68 60 40 20 0 -20 -10 0 0 + F 0 0 0 0 0 5 10 20 26 10 -10 -30 -60 -65 -60 -40 -20 + Y 0 5 10 15 20 25 30 35 40 35 30 25 20 15 10 5 0 + W 0 0 0 0 0 -10 -10 -10 -10 -10 -10 -15 -20 -25 -30 -20 -10 + - 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + X 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + ? 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + DIRECTIONAL INFORMATION MEASURE FOR TURNS + J-8 J-7 J-6 J-5 J-4 J-3 J-2 J-1 J J+1 J+2 J+3 J+4 J+5 J+6 J+7 J+8 + C 20 40 50 60 60 55 50 45 44 40 35 30 25 20 15 10 5 + S 0 -10 -20 -20 10 15 20 25 26 25 20 15 10 0 0 0 0 + T 0 10 20 20 20 15 18 5 3 5 10 15 20 20 20 10 0 + P 10 20 30 40 50 70 10 -90 36 90 10 0 0 0 0 0 0 + A 0 0 0 -10 -20 -30 -40 -50 -50 -40 -30 -20 -10 0 0 0 0 + G 0 0 0 0 10 30 55 55 57 40 0 0 0 0 0 0 0 + N 0 0 0 10 20 30 35 40 42 40 35 30 20 10 5 0 0 + D 0 0 0 0 0 0 5 10 31 10 5 0 0 0 0 0 0 + E 0 -5 -10 -15 -20 -30 -40 -45 -47 -20 0 10 5 0 0 0 0 + Q 10 20 30 25 20 15 10 5 4 20 30 40 50 60 50 40 20 + B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + Z 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + H 0 0 0 0 0 0 0 0 -3 0 10 20 30 20 10 0 0 + R 0 0 0 0 0 0 0 10 21 30 40 30 20 10 0 0 0 + K -10 -20 -30 -40 -25 -10 0 10 10 10 0 -20 -30 -20 -10 -5 0 + M -5 -15 -20 -25 -30 -35 -40 -45 -48 -45 -40 -35 -30 -25 -20 -15 -5 + I 0 0 0 0 0 -10 -20 -30 -46 -40 -10 0 0 20 30 20 10 + L 0 0 0 -10 -20 -30 -40 -50 -56 -20 -10 0 0 0 0 0 0 + V 0 0 0 0 -10 -20 -30 -40 -60 -40 -30 -20 -10 0 0 0 0 + F 0 0 0 0 0 -5 -10 -15 -18 -15 0 15 30 25 20 10 0 + Y 0 0 0 5 15 15 20 25 29 25 20 15 15 5 0 0 0 + W 0 0 0 10 20 30 40 80 36 -30 30 40 50 60 70 40 20 + - 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + X 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + ? 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + DIRECTIONAL INFORMATION MEASURE FOR COIL + J-8 J-7 J-6 J-5 J-4 J-3 J-2 J-1 J J+1 J+2 J+3 J+4 J+5 J+6 J+7 J+8 + C 0 0 0 0 0 0 -10 -30 -47 -30 -10 0 0 0 0 0 0 + S 0 -10 -20 -20 10 15 20 25 50 25 20 15 10 0 0 0 0 + T 0 10 20 30 20 15 10 15 17 15 10 15 20 30 20 10 0 + P 0 0 10 20 30 40 50 55 58 50 10 0 0 0 0 0 0 + A 0 0 0 0 -5 -10 -20 -25 -25 -25 -20 -15 -10 -5 0 0 0 + G 0 0 0 0 10 30 40 45 49 45 40 30 10 0 0 0 0 + N 0 0 0 10 20 30 35 40 46 40 35 30 20 10 0 0 0 + D 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + E 0 0 10 20 40 20 0 -10 -44 -40 -20 -10 0 0 0 0 0 + Q 10 20 30 25 20 15 10 0 -5 20 30 40 50 60 50 40 20 + B 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + Z 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + H 0 0 0 0 0 0 0 10 16 15 10 10 10 10 5 0 0 + R 0 0 0 0 0 0 0 0 -12 0 20 30 20 10 0 0 0 + K -10 -20 -30 -40 -25 -20 -10 -8 -8 0 0 -20 -30 -20 -10 -5 0 + M 0 -5 -10 -15 -20 -25 -30 -40 -41 -40 -30 -25 -20 -15 -10 -5 0 + I 0 0 0 0 0 -10 -20 -30 -33 -30 -10 0 10 20 30 20 0 + L 0 0 0 -10 -20 -30 -40 -30 -20 -20 -10 0 0 0 0 0 0 + V 0 0 0 0 -10 -20 -25 -30 -35 -30 -25 -20 -10 0 0 0 0 + F 0 0 0 0 0 -5 -10 -20 -41 -20 0 15 30 25 20 10 0 + Y 0 0 0 0 0 0 0 0 -6 0 0 0 0 0 0 0 0 + W 0 0 0 10 20 30 40 20 12 20 30 40 50 60 70 40 20 + - 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + X 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + ? 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 + 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 diff --git a/tables/SAP.MRG b/tables/SAP.MRG new file mode 100644 index 0000000..b9e90fd --- /dev/null +++ b/tables/SAP.MRG @@ -0,0 +1,10 @@ +PLOT POSITIONS FOR SAP. DRAWING BOARD SIZE, X0 XLENGTH, OPT Y0 YLEN + 10000 10000 + 1100 8500 + 12 5000 5000 + 13 1 100 + 15 1 10000 + 16 20 20 + 29 4000 1000 + 33 5000 4000 + 34 9000 1000 diff --git a/tables/SIP.MRG b/tables/SIP.MRG new file mode 100644 index 0000000..6b30f89 --- /dev/null +++ b/tables/SIP.MRG @@ -0,0 +1,4 @@ +PLOT POSITIONS FOR DIAGON. DRAWING BOARD SIZE, X0 XLENGTH, OPT Y0 YLEN + 10000 10000 + 2400 7000 7000 + 1 10 9980 diff --git a/tables/TAGDB b/tables/TAGDB new file mode 100644 index 0000000..f96b0a6 --- /dev/null +++ b/tables/TAGDB @@ -0,0 +1,60 @@ +# +# This is a tag database for xdap +# +# The syntax in BNF is: +# +# ::= | +# ::= : \n | \n +# ::=

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sequence on a graph widget + Last update: Friday 15 May 1992 +*/ + + +/* + `plotEdgeOffset' indicates the pixel position at which point 0 + occurs. The characters for bases are printed with their centres + corresponding to their locations on the plot. + + The cutoffs are actually drawn between the positions of the + bases on either side. + + SD: + Added incScaleFactor, decScaleFactor + Changed scaleTrace to use scale factor + SD: + Support monochrome displays when marking cutoffs + SD: 15-May-1992 + Calculation of next value of pSegN in drawTrace() gives a value one too many + +*/ + + + + +/* ---- Includes ---- */ + +#include "traceDisplay.h" +#include "display.h" /* IMPORT: userEvent */ +#include "Graph.h" +#include "seq.h" /* IMPORT: Seq, NULLSeq, getNPoints */ + + +#include +#include +#include + + + +/* ---- Statics ---- */ + +static Seq currSeq = NULLSeq; +static int NPoints; +static TRACE maxTraceVal; + +static Widget traceWid; + +static Dimension traceWidth; /* Current width of the trace graph */ +static Dimension traceHeight; /* Current height of the trace graph */ +static Dimension plotEdgeOffset; +static Dimension plotWidth; /* Current width of the plot + = graphWidth - 2*plotEdgeOffset */ + +static int leftCutoff, rightCutoff; + +static GC Agc, Cgc, Ggc, Tgc; +static Pixel normBackground, dimBackground; + +/* monochrome stippling */ +static GC greygc; +static int planes; + +static float scaleFactor; + + + + +/* ---- Position translation ---- */ + +/* + Convert a value from the `point-in-the-sequence' to the + `x-pixel-on-the-graph' scale, and vice-versa, given that + `plotWidth' holds the current pixel width of the graph which + starts `plotEdgeOffset' from either edge of the graph and + `NPoints' gives the current point width. + Fractional results are truncated - for pixelToPoint this may + mean a point one too low is indicated, but a point too high is + never indicated. +*/ + + +static int pointToPixel(int point) +{ return(((point * plotWidth) / (NPoints-1)) + plotEdgeOffset); +} + + +static int pixelToPoint(int pixel) +{ return(((pixel-plotEdgeOffset) * (NPoints-1)) / plotWidth); +} + + +static int scaleTrace(int y) +{ return(traceHeight - + (int)(scaleFactor * (float)y * (float)traceHeight / (float)maxTraceVal) + ); +} + + +static int leftCutoffPoint(int baseNum) +/* + Return the point corresponding to a left cutoff at `baseNum'. +*/ +{ int pL, pR; + + pL = (baseNum == 0) ? 0 : baseNumToPoint(currSeq, EdBases, baseNum-1); + pR = (baseNum == 0) ? 0 : baseNumToPoint(currSeq, EdBases, baseNum); + + return((pR==NULLPoint)?pL:(pL+pR)/2); +} + + +static int rightCutoffPoint(int baseNum) +/* + Return the point corresponding to a right cutoff at `baseNum'. +*/ +{ int NedBases = getNBases(currSeq, EdBases); + int pL, pR; + + pL = (baseNum == 0) + ? NPoints-1 + : baseNumToPoint(currSeq, EdBases, NedBases-baseNum); + pR = (baseNum == 0) + ? NPoints-1 + : baseNumToPoint(currSeq, EdBases, NedBases-baseNum-1); + + return((pL+pR)/2); +} + + + + + +/* ---- Internal routines ---- */ + + + + +static void drawTrace(Widget wid, char base, int p0, int pN) +/* + Draw the `base' trace between points p0..pN (inclusive) +*/ +{ GC gc; + int pSeg, pSeg0, pSegN; +#define NSegs 500 + int traces[NSegs]; + XPoint xPoint[NSegs]; + + switch (base) + { case 'A': gc=Agc; break; + case 'C': gc=Cgc; break; + case 'G': gc=Ggc; break; + case 'T': gc=Tgc; break; + } + + + /* + The range (p0..pN) is drawn as a series of overlapping + segments (pSeg0..pSegN). + */ + pSegN = p0; + do + { int i; + + /* + This segment carries on from and including the last point + of the last segment. + */ + pSeg0 = pSegN; + pSegN = ((pSeg0+NSegs-1)<=pN) ? pSeg0+NSegs-1 : pN; + /* Get the traces for this segment */ + getTraces(currSeq, base, pSeg0, pSegN, traces); + + /* Translate to pixel coords and place in an XPoint array */ + pSeg = pSeg0; + i = 0; + while (pSeg<=pSegN) + { xPoint[i].x = pointToPixel(pSeg); + xPoint[i].y = scaleTrace(traces[i]); + pSeg++; + i++; + } + + /* Draw the segments in the XPoint array */ + XDrawLines(XtDisplay(wid), + XtWindow(wid), + gc, + xPoint, + pSegN-pSeg0+1, + CoordModeOrigin); + } while (pSegN < pN); +} + + + + + +/* ---- Callbacks ---- */ + + + + +static void exposeCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ Region region = (Region) call_data; + XRectangle rect; + int x0, xN; /* Affected region: pixels */ + int p0, pN; /* Affected region: points */ + int leftCutoffP, rightCutoffP; + + + if ((currSeq == NULLSeq) || isBasesOnly(currSeq)) return; + + + /* + `region' gives us the exposed graph region. + XClipBox will return a bounding rectangle, in pixels. + */ + XClipBox(region, &rect); + x0 = rect.x; + xN = rect.x+rect.width; + + /* Trim it so we only consider the plot proper */ + if ((x0>plotWidth+plotEdgeOffset) || (xNplotWidth+plotEdgeOffset) ? plotWidth+plotEdgeOffset : xN; + + /* Convert to affected points */ + p0 = pixelToPoint(x0); + pN = pixelToPoint(xN); + if (pNrightCutoffP) + /* + Some of the exposed region is in the right cutoff area. + Repaint it with dimmed background. + */ + { int firstP = (p0>rightCutoffP) ? p0 : rightCutoffP; + int lastP = pN; + + int pix0 = pointToPixel(firstP); + int pixN = pointToPixel(lastP); + + if (planes == 1) { + XFillRectangle(XtDisplay(traceWid), XtWindow(traceWid), + greygc, + pix0, 0, + pixN-pix0, traceHeight); + } else { + XSetWindowBackground(XtDisplay(traceWid), XtWindow(traceWid), + dimBackground); + XClearArea(XtDisplay(traceWid), XtWindow(traceWid), + pix0, 0, + pixN-pix0, traceHeight, + False); + XSetWindowBackground(XtDisplay(traceWid), XtWindow(traceWid), + normBackground); + } + } + + /* + Now draw the traces. + The GC background is unused when drawing lines, so we do + not need to change it depending on whether we are in the + cutoff region or not. + */ + drawTrace(wid, 'A', p0, pN); + drawTrace(wid, 'C', p0, pN); + drawTrace(wid, 'G', p0, pN); + drawTrace(wid, 'T', p0, pN); +} + + + + +static void userCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +/* + Handles all XtNcallbacks, i.e., buttons and keys. +*/ +{ XEvent *eventP = (XEvent *) call_data; + + if (currSeq == NULLSeq) return; + + /* + Callback to the `display' module. + */ + userEvent(eventP); +} + + + + +static void resizeCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ Arg args[10]; + int nargs; + + /* + This routine can be called in two ways. + (a) as a callback when the trace is resized by the user + (b) indirectly when setGraphWidth alters the graph width + */ + nargs = 0; + XtSetArg(args[nargs], XtNheight, &traceHeight); nargs++; + XtSetArg(args[nargs], XtNwidth, &traceWidth); nargs++; + XtGetValues(wid, args, nargs); + + if (XtIsRealized(wid)) XClearWindow(XtDisplay(wid), XtWindow(wid)); +} + + + + +/* ---- Exports ---- */ + + +void redisplayTraces() +{ + /* + ** Clear the area and generate an expose. + */ + XClearArea(XtDisplay(traceWid), XtWindow(traceWid), + 0, 0, + 0, 0, + True); +} + +void incScaleFactor() +{ + scaleFactor *= 1.5; + redisplayTraces(); +} + +void decScaleFactor() +{ + scaleFactor /= 1.5; + redisplayTraces(); +} + +void setScaleFactor(float sf) +{ + scaleFactor = sf; + redisplayTraces(); +} + + + +void createTraceDisplay(Widget parentWid, Widget fromVertWid, + Dimension width, Dimension offset) +/* + Create the trace display within the Form widget `parent', + with the XtNfromVert constraint `fromVertWid'. The initial + width is `width' and the plot is drawn `offset' from + the left and right hand edges. + No trace is initially displayed. +*/ +{ Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNwidth, width); nargs++; + traceWid = XtCreateManagedWidget("trace", graphWidgetClass, + parentWid, args, nargs); + XtAddCallback(traceWid, XtNcallback, userCallback, NULL); + XtAddCallback(traceWid, XtNexposeCallback, exposeCallback, NULL); + XtAddCallback(traceWid, XtNresizeCallback, resizeCallback, NULL); + + nargs = 0; + XtSetArg(args[nargs], XtNheight, &traceHeight); nargs++; + XtGetValues(traceWid, args, nargs); + traceWidth = width; + plotWidth = traceWidth-(2*plotEdgeOffset); + plotEdgeOffset = offset; + scaleFactor = 1.0; + + currSeq = NULLSeq; + + /* + Get the GCs + */ + Agc = GraphGC1(traceWid); + Cgc = GraphGC2(traceWid); + Ggc = GraphGC3(traceWid); + Tgc = GraphGC4(traceWid); + + /* + Get the backgrounds + */ + nargs = 0; + XtSetArg(args[nargs], XtNbackground, &normBackground); nargs++; + XtSetArg(args[nargs], XtNdimBackground, &dimBackground); nargs++; + XtGetValues(traceWid, args, nargs); + + planes = DisplayPlanes(XtDisplay(traceWid),DefaultScreen(XtDisplay(traceWid))); + if (planes == 1) { + /* prepare for monochrome display */ + XtGCMask valuemask = (GCStipple | GCFillStyle); + XGCValues values; + Pixmap grey_stipple; + +#define grey_width 4 +#define grey_height 4 + static char grey_bits[] = { + 0x0e, 0x0b, 0x0e, 0x0b}; + + grey_stipple = + XCreateBitmapFromData(XtDisplay(traceWid), + RootWindowOfScreen(XtScreenOfObject(traceWid)), + grey_bits, + grey_width, + grey_height); + + values.stipple = grey_stipple; + values.fill_style = FillOpaqueStippled; + greygc = XCreateGC(XtDisplayOfObject(traceWid), + RootWindowOfScreen(XtScreenOfObject(traceWid)), + valuemask, &values); + + } + +} + + + + +void displayTrace(Seq seq) +/* + Display the trace of sequence `seq'. +*/ +{ + if (seq == NULLSeq) return; + currSeq = seq; + + + /* + Get static information about the current sequence. + */ + NPoints = getNPoints(currSeq); + maxTraceVal = getMaxTraceVal(currSeq); + getCutoffs(currSeq, &leftCutoff, &rightCutoff); +} + + + + +void unDisplayTrace() +/* + Cease displaying the current sequence (if any). +*/ +{ currSeq = NULLSeq; +} + + + + +void setTraceWidth(Dimension width) +/* + Set the width of the current sequence trace display. +*/ +{ Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNwidth, width); nargs++; + XtSetValues(traceWid, args, nargs); + + traceWidth = width; + plotWidth = traceWidth-(2*plotEdgeOffset); + + /* + Clear the graph. + */ + if (XtIsRealized(traceWid)) + XClearWindow(XtDisplay(traceWid), XtWindow(traceWid)); +} + + + + +void traceCutoffChange() +/* + One or both of the cutoffs have changed. + Update our display. +*/ +{ int newLeftCutoff, newRightCutoff; + + getCutoffs(currSeq, &newLeftCutoff, &newRightCutoff); + + + if (newLeftCutoff != leftCutoff) + /* + The region between newLeftCutoff and leftCutoff has changed. + */ + { int b0, bN; + int p0, pN; + int pix0, pixN; + + /* + b0 = leftmost base number. bN = rightmost base number. + */ + if (newLeftCutoff /* IMPORT: Widget, Dimension */ + + + + +/* ---- Exports ---- */ + + +extern void createTraceDisplay(Widget parentWid, Widget fromVertWid, + Dimension width, Dimension offset); +/* + Create the trace display within the Form widget `parent', + with the XtNfromVert constraint `fromVertWid'. The initial + width is `width' and the plot is drawn `plotEdgeOffset' from + the left and right hand edges. + No trace is initially displayed. +*/ + + +extern void displayTrace(Seq seq); +/* + Display the trace of sequence `seq'. +*/ + + +extern void unDisplayTrace(); +/* + Cease displaying the trace of the current sequence (if any). +*/ + + +extern void setTraceWidth(Dimension width); +/* + Set the width of the current sequence trace display. +*/ + + +extern void traceCutoffChange(); +/* + One or both of the cutoffs have changed. + Update our display. +*/ + + +extern void incScaleFactor(); +/* + Increment vertical scaling of trace +*/ + + +extern void decScaleFactor(); +/* + Decrement vertical scaling of trace +*/ + + + +extern void setScaleFactor(float sf); +/* + Set vertical scaling of trace +*/ diff --git a/src/newted/traceType.c b/src/newted/traceType.c new file mode 100644 index 0000000..2e55d2c --- /dev/null +++ b/src/newted/traceType.c @@ -0,0 +1,119 @@ +/* + Title: traceType + + File: traceType.c + Purpose: determining trace format + + Last update: Wed Nov 11 1992 + + Change log :- +*/ + +/* ---- Imports ---- */ + +#include "traceType.h" + + +/* ---- Privates ---- */ +struct { + int type; + int offset; + char *string; +} magics[] = { + { TT_SCF , 0, ".scf" } , + { TT_ABI , 0, "ABIF" } , + { TT_ALF , 518, "ALF " } , + { TT_SCF , 0, "\234\330\300\000" } /* Amersham variant */ +}; + + + +char *trace_types[5] = { + "unknownFmt", + "scfFmt", + "abiFmt", + "alfFmt", + "plainFmt" + }; + + +#define Number(A) ( sizeof(A) / sizeof((A)[0]) ) + + + +int determine_trace_type(char *fn) +{ + FILE *fp; + int i; + int len; + char buf[512]; + int ps; + int acgt; + int c; + + + if ( (fp = fopen(fn,"rb")) == NULL ) return TT_EEK; + + /* determine if this is a text file */ + len = 0; ps = 0; acgt = 0; + for (i = 0; i < 512; i++) { + if ( ( c = fgetc(fp) ) == EOF ) break; + switch(c) { + case 'a': case 'c': case 'g': case 't': + case 'A': case 'C': case 'G': case 'T': + /*YUK! need the next line?*/ + case 'n': case 'N': case '-': + acgt++; + default: + len++; + if ( isprint(c) || isspace(c) ) ps++; + } + } + /*YUK! 75% of characters printable means text*/ + if ( 100 * ps > 75 * len ) { + fclose(fp); + /*YUK! 75% of printables ACGTN means plain*/ + return ( 100 * acgt > 75 * ps ) ? TT_PLN : TT_UNK; + } + + /* YUK! short files are not traces? */ + if (len<512) { + fclose(fp); + return TT_UNK; + } + + /* check magics */ + for (i = 0 ; i < Number(magics) ; i++) { + if (fseek(fp,magics[i].offset,0) == 0) { + len = strlen(magics[i].string); + if (fread(buf,len,1,fp)==1) { + if (strncmp(buf,magics[i].string,len)==0) { + fclose(fp); + return magics[i].type; + } + } + } + } + + fclose(fp); + return TT_UNK; + +} + +char *traceType(char *traceName) +{ + char *t; + switch(determine_trace_type(traceName)) { + case TT_UNK: t = "UNK"; break; + case TT_SCF: t = "SCF"; break; + case TT_ABI: t = "ABI"; break; + case TT_ALF: t = "ALF"; break; + case TT_PLN: t = "PLN"; break; + case TT_EEK: + default: + t = "EEK"; break; + } + return t; + +} + diff --git a/src/newted/traceType.h b/src/newted/traceType.h new file mode 100644 index 0000000..6449c5b --- /dev/null +++ b/src/newted/traceType.h @@ -0,0 +1,39 @@ +#ifndef _traceType_h +#define _traceType_h + +/* + Title: traceType.h + + File: traceType.h + Purpose: determining traceType of traces + Last update: Tue Jan 15 1991 + + Change log :- +*/ + +/* ---- Imports ---- */ + +#include /* IMPORT: fopen, fclose, fseek, ftell, fgetc */ +#include +#include /* IMPORT: isprint*/ + +/* ---- Exports ---- */ +#define TT_EEK -1 +#define TT_UNK 0 +#define TT_SCF 1 +#define TT_ABI 2 +#define TT_ALF 3 +#define TT_PLN 4 + +extern char *trace_types[5]; + +extern int determine_trace_type(char *fn); + +extern char *traceType(char *traceName); +/* returns the traceType, e.g. ALF, PLN, ABI..., when sent +a tracename */ + +#endif /*_traceType_h*/ + + + diff --git a/src/newted/values.h b/src/newted/values.h new file mode 100644 index 0000000..792e966 --- /dev/null +++ b/src/newted/values.h @@ -0,0 +1,118 @@ +/* @(#)values.h 1.7 89/10/02 SMI; from S5R2 1.16 */ + +#ifndef _values_h +#define _values_h + +/* These values work with any binary representation of integers + * where the high-order bit contains the sign. */ + +/* a number used normally for size of a shift */ +#if gcos +#define BITSPERBYTE 9 +#else +#define BITSPERBYTE 8 +#endif +#define BITS(type) (BITSPERBYTE * (int)sizeof(type)) + +/* short, regular and long ints with only the high-order bit turned on */ +#define HIBITS ((short)(1 << BITS(short) - 1)) +#define HIBITI (1 << BITS(int) - 1) +#define HIBITL (1L << BITS(long) - 1) + +/* largest short, regular and long int */ +#define MAXSHORT ((short)~HIBITS) +#define MAXINT (~HIBITI) +#define MAXLONG (~HIBITL) + +/* various values that describe the binary floating-point representation + * _EXPBASE - the exponent base + * DMAXEXP - the maximum exponent of a double (as returned by frexp()) + * FMAXEXP - the maximum exponent of a float (as returned by frexp()) + * DMINEXP - the minimum exponent of a double (as returned by frexp()) + * FMINEXP - the minimum exponent of a float (as returned by frexp()) + * MAXDOUBLE - the largest double + ((_EXPBASE ** DMAXEXP) * (1 - (_EXPBASE ** -DSIGNIF))) + * MAXFLOAT - the largest float + ((_EXPBASE ** FMAXEXP) * (1 - (_EXPBASE ** -FSIGNIF))) + * MINDOUBLE - the smallest double (_EXPBASE ** (DMINEXP - 1)) + * MINFLOAT - the smallest float (_EXPBASE ** (FMINEXP - 1)) + * DSIGNIF - the number of significant bits in a double + * FSIGNIF - the number of significant bits in a float + * DMAXPOWTWO - the largest power of two exactly representable as a double + * FMAXPOWTWO - the largest power of two exactly representable as a float + * _IEEE - 1 if IEEE standard representation is used + * _DEXPLEN - the number of bits for the exponent of a double + * _FEXPLEN - the number of bits for the exponent of a float + * _HIDDENBIT - 1 if high-significance bit of mantissa is implicit + * LN_MAXDOUBLE - the natural log of the largest double -- log(MAXDOUBLE) + * LN_MINDOUBLE - the natural log of the smallest double -- log(MINDOUBLE) + * LN_MAXFLOAT - the natural log of the largest float + * LN_MINFLOAT - the natural log of the smallest float + */ +#if u3b || u3b5 || sun +#define MAXDOUBLE 1.797693134862315708e+308 +#define MAXFLOAT ((float)3.40282346638528860e+38) +#define MINDOUBLE 4.94065645841246544e-324 +#define MINFLOAT ((float)1.40129846432481707e-45) +#define _IEEE 1 +#define _DEXPLEN 11 +#define _HIDDENBIT 1 +#define DMINEXP (-(DMAXEXP + DSIGNIF - _HIDDENBIT - 3)) +#define FMINEXP (-(FMAXEXP + FSIGNIF - _HIDDENBIT - 3)) +#define LN_MAXFLOAT 8.8722839052068e+01 +#define LN_MINFLOAT -1.03278929903432e+02 +#endif +#if pdp11 || vax +#define MAXDOUBLE 1.701411834604692293e+38 +#define MAXFLOAT ((float)1.701411733192644299e+38) +/* The following is kludged because the PDP-11 compilers botch the simple form. + The kludge causes the constant to be computed at run-time on the PDP-11, + even though it is still "folded" at compile-time on the VAX. */ +#define MINDOUBLE (0.01 * 2.938735877055718770e-37) +#define MINFLOAT ((float)MINDOUBLE) +#define _IEEE 0 +#define _DEXPLEN 8 +#define _HIDDENBIT 1 +#define DMINEXP (-DMAXEXP) +#define FMINEXP (-FMAXEXP) +#endif +#if gcos +#define MAXDOUBLE 1.7014118346046923171e+38 +#define MAXFLOAT ((float)1.7014118219281863150e+38) +#define MINDOUBLE 2.9387358770557187699e-39 +#define MINFLOAT ((float)MINDOUBLE) +#define _IEEE 0 +#define _DEXPLEN 8 +#define _HIDDENBIT 0 +#define DMINEXP (-(DMAXEXP + 1)) +#define FMINEXP (-(FMAXEXP + 1)) +#endif +#if u370 +#define _LENBASE 4 +#else +#define _LENBASE 1 +#endif +#define _EXPBASE (1 << _LENBASE) +#define _FEXPLEN 8 +#define DSIGNIF (BITS(double) - _DEXPLEN + _HIDDENBIT - 1) +#define FSIGNIF (BITS(float) - _FEXPLEN + _HIDDENBIT - 1) +#define DMAXPOWTWO ((double)(1L << BITS(long) - 2) * \ + (1L << DSIGNIF - BITS(long) + 1)) +#define FMAXPOWTWO ((float)(1L << FSIGNIF - 1)) +#define DMAXEXP ((1 << _DEXPLEN - 1) - 1 + _IEEE) +#define FMAXEXP ((1 << _FEXPLEN - 1) - 1 + _IEEE) +#define LN_MAXDOUBLE (M_LN2 * DMAXEXP) +#define LN_MINDOUBLE (M_LN2 * (DMINEXP - 1)) + +#define H_PREC (DSIGNIF % 2 ? (1L << DSIGNIF/2) * M_SQRT2 : 1L << DSIGNIF/2) +#define X_EPS (1.0/H_PREC) +#define X_PLOSS ((double)(long)(M_PI * H_PREC)) +#define X_TLOSS (M_PI * DMAXPOWTWO) +#define M_LN2 0.69314718055994530942 +#define M_PI 3.14159265358979323846 +#define M_SQRT2 1.41421356237309504880 +#define MAXBEXP DMAXEXP /* for backward compatibility */ +#define MINBEXP DMINEXP /* for backward compatibility */ +#define MAXPOWTWO DMAXPOWTWO /* for backward compatibility */ + +#endif /*!_values_h*/ diff --git a/src/scf/README b/src/scf/README new file mode 100644 index 0000000..92c369c --- /dev/null +++ b/src/scf/README @@ -0,0 +1,29 @@ +Creating SCF files from ABI 373A and Pharmacia A.L.F. trace files +----------------------------------------------------------------- + +SCF files can be created from existing trace files using the program +makeSCF. Typically, SCF files take up a mere 30% of the space of ABI +373A traces. + +Usage: + + makeSCF {-ABI | -ALF} tracefile -output scffile + +Where: + tracefile is the file you wish to convert +and + scffile is the name of the SCF file that will be created. + + +Example: + +To convert an ABI 373A trace: + makeSCF -ABI trace.abi -output trace.scf + +To convert a Pharmacia A.L.F. trace: + makeSCF -ALF trace.alf -output trace.scf + + +NOTE: +The Pharmacia A.L.F. traces referred to here are those created by +the program alfsplit. diff --git a/src/scf/SCF-SPEC.V2.0 b/src/scf/SCF-SPEC.V2.0 new file mode 100644 index 0000000..64f2efb --- /dev/null +++ b/src/scf/SCF-SPEC.V2.0 @@ -0,0 +1,218 @@ + A Proposed Standard File Format for Chromatograms + (Version 2, Revision 0) + + Simon Dear + (sd@mrc-lmb.cam.ac.uk) + Medical Research Council + Laboratory of Molecular Biology + 18 November 1992 + + + + +0. Introduction + +We seek to establish a standard chromatogram file format that will be +supported by all sequencing machine manufacturers and software +developers. Our software supports data in this format. We will +encourage other software developers to do the same, and equipment +manufacturers to provide either files in the format, or conversion +software to produce them. + + +1. The Header Record + +The file begins with a 128 byte header record that describes the +location and size of the chromatogram data in the file. Nothing is +implied about the order in which the components (samples, sequence and +comments) appear. The version field is a character string +respresenting the version and revision of the SCF format. The current +value of this field is "2.00" + +/* +** Type definition for the Header structure +*/ +#define SCF_MAGIC (((((long)'.'<<8)+(long)'s'<<8)+(long)'c'<<8)+(long)'f') +typedef struct { + long magic_number; + long sample; /* Number of elements in Samples matrix */ + long samples_offset; /* Byte offset from start of file */ + long bases; /* Number of bases in Bases matrix */ + long bases_left_clip; /* Number of bases in left clip (vector)*/ + long bases_right_clip; /* Number of bases in right clip (unreliable) */ + long bases_offset; /* Byte offset from start of file */ + long comments_size; /* Number of bytes in Comment section */ + long comments_offset; /* Byte offset from start of file */ + char version[4]; /* "version.revision" */ + long sample_size; /* Size of samples in bytes 1=8bits, 2=16bits*/ + long code_set; /* code set used */ + long spare[20]; /* Unused */ +} Header; + +NOTE: Features new to version 2 + +For versions of SCF files 2.0 or greater (Header.version >= "2.00"), +the version number, precision of data, the uncertain code set are +specified in the header. Otherwise, the precision is assumed to be 1 +byte, and the code set to be the default code set. + +The following uncertainty code sets are recognised. People wishing to +use others with the SCF format should register them with the author of +this document. + + 0 {A,C,G,T,-} (default) + 1 Staden + 2 IUPAC (NC-IUB) + 3 Pharmacia A.L.F. (NC-IUB) + 4 {A,C,G,T,N} (ABI 373A) + 5 IBI/Pustell + 6 DNA* + 7 DNASIS + 8 IG/PC-Gene + 9 MicroGenie + + +2. The Sample Points. + +The trace information is stored at byte offset Header.samples_offset +from the start of the file. For each sample point there are values for +each of the four bases. Header.sample_size holds the precision of the +sample values. The precision must be one of "1" (unsigned byte) and +"2" (unsigned short). The sample points need not be normalised to any +particular value, though it is assumed that they represent positive +values. This is, they are of unsigned type. + +/* +** Type definition for the Sample data +*/ + +typedef struct { + unsigned char sample_A; /* Sample for A trace */ + unsigned char sample_C; /* Sample for C trace */ + unsigned char sample_G; /* Sample for G trace */ + unsigned char sample_T; /* Sample for T trace */ +} Samples1; +typedef struct { + unsigned short sample_A; /* Sample for A trace */ + unsigned short sample_C; /* Sample for C trace */ + unsigned short sample_G; /* Sample for G trace */ + unsigned short sample_T; /* Sample for T trace */ +} Samples2; + +NOTE: Features new to version 2 +The samples are no longer restricted to 8 bit values. + + +3. Sequence Information. + +Information relating to the base interpretation of the trace is stored +at byte offset Header.bases_offset from the start of the file. Stored +for each base are: its character representation and a number (an index +into the Samples data structure) indicating its position within the +trace. The relative probabilities of each of the 4 bases occuring at +the point where the base is called can be stored in prob_A, prob_C, +prob_G and prob_T. + +The Amersham FilmReader uses Bases.spare[0] to store the confidence +level of the base. The value ranges from 0 (low confidence) to 8 +(high), and 9 indicating the base has been manually edited. + +/* +** Type definition for the sequence data +*/ +typedef unsigned char byte; +typedef struct { + long peak_index; /* Index into Samples matrix for base position */ + byte prob_A; /* Probability of it being an A */ + byte prob_C; /* Probability of it being an C */ + byte prob_G; /* Probability of it being an G */ + byte prob_T; /* Probability of it being an T */ + char base; /* Called base character */ + byte spare[3]; /* Spare */ +} Base; + + + +4. Comments. + +Comments are stored at offset Header.comments_offset from the start of +the file. Lines in this section are of the format: + + = + + can be any string, though several have special meaning and +their use is encouraged. + +ID Field Example +---- ------------------------ ---------------- +MACH Sequencing machine model MACH=Pharmacia A.L.F. +TPSW Trace processing software version TPSW=A.L.F. Analysis Program, Version=1.67 +BCSW Base calling software version BCSW=A.L.F. Analysis Program, Version=1.67 +DATF Data source format DATF=AM_Version=2.0 +DATN Data source name DATN=a10c.alf +CONV Format conversion software CONV=makeSCF v2.0 + +Other fields might include: + +ID Field Example +---- ------------------------ ---------------- +OPER Operator OPER=sd +STRT Time run started STRT=Aug 05 1991 12:25:01 +STOP Time run stopped STOP=Aug 05 1991 16:26:25 +PROC Time processed PROC=Aug 05 1991 18:50:13 +EDIT Time edited EDIT=Aug 05 1991 19:06:18 +NAME Sample name NAME=a21b1.s1 +SIGN Average signal strength SIGN=A=56,C=66,G=13,T=18 +SPAC Average base spacing SPAC=12.04 +SCAL Factor used in scaling traces SCAL=0.5 + + +/* +** Type definition for the comments +*/ +typedef char Comments; /* Zero terminated list of \n separated entries */ + +Appendix: Byte ordering and integer representation + +"Forward byte and reverse bit" ordering will be used for all integer +values. This is the same as used in the MC680x0 and SPARC processors, +but the reverse of the byte ordering used on the VAX and Intel 80x86 +processors. + + + Off+0 Off+1 + +-------+-------+ +Short | MSB | LSB | + +-------+-------+ + + Off+0 Off+1 Off+2 Off+3 + +-------+-------+-------+-------+ +Long | MSB | ... | ... | LSB | + +-------+-------+-------+-------+ + + + +To read integers on systems with any byte order use something like this: + +short read_short(FILE *fp) +{ + unsigned char buf[sizeof(short)]; + + fread(buf, sizeof(buf), 1, fp); + return (short) + (((unsigned short)buf[1]) + + ((unsigned short)buf[0]<<8)); +} + +long read_long(FILE *fp) +{ + unsigned char buf[sizeof(long)]; + + fread(buf, sizeof(buf), 1, fp); + return (long) + (((unsigned long)buf[3]) + + ((unsigned long)buf[2]<<8) + + ((unsigned long)buf[1]<<16) + + ((unsigned long)buf[0]<<24)); +} + diff --git a/src/scf/makeSCF.c b/src/scf/makeSCF.c new file mode 100644 index 0000000..b9441f6 --- /dev/null +++ b/src/scf/makeSCF.c @@ -0,0 +1,157 @@ +/* + Title: makeSCF + + File: makeSCF.c + Purpose: Main module + Last update: Thursday 11 July 1991 + + Change log: + +*/ + + +/* +*/ + + + +/* ---- Includes ---- */ + +#include "seq.h" /* IMPORT: Seq, NULLBaseNum */ + +#include /* IMPORT: stderr, fprintf */ +#include "seqIOABI.h" +#include "seqIOALF.h" +#include "seqIOSCF.h" +#include "seqIOPlain.h" +#include "mystdlib.h" /* IMPORT: exit */ +#include "writeSCF.h" + + +/* ---- Static variables ---- */ + +/* + Where to put the command line arguments when we dig them + out of the resources. +*/ +#define trace_unknown 0 +#define trace_SCF 1 +#define trace_ALF 2 +#define trace_ABI 3 +#define trace_Plain 4 + +typedef struct +{ + char * fn; + int type; + char * output; +} Arguments; + + +/* ---- Callbacks ---- */ + + + + + +/* ---- Internal functions ---- */ + + +static void usage() +{ + + fprintf(stderr,"usage: makeSCF [(-ABI | -ALF) {tracefilename} -output {outputfilename}\n"); + +} + + + + + +/* ---- Exported functions ---- */ + + +void main(unsigned int argc, char **argv) +{ + Arguments args; + Seq currSeq; + int in, out; + + /* parse command line arguments here */ + + args.fn = NULL; + args.type = trace_unknown; + args.output = NULL; + in = out = 0; + + for(argc--,argv++; argc>0 ; argc--,argv++) { + + if (strcmp(*argv,"-ABI") == 0) { + args.type = trace_ABI; + args.fn = *++argv; argc--; + in++; + } else if (strcmp(*argv,"-ALF") == 0) { + args.type = trace_ALF; + args.fn = *++argv;argc--; + in++; + } else if (strcmp(*argv,"-SCF") == 0) { + args.type = trace_SCF; + args.fn = *++argv;argc--; + in++; + } else if (strcmp(*argv,"-output") == 0) { + args.output = *++argv; argc--; + out++; + } else { + usage(); + fprintf(stderr,"switch not recognised\n"); + exit(1); + } + + } + + + + + /* + Die if bad options given. + */ + if (in!=1 || out!=1 || args.fn==NULL ) { + usage(); + fprintf(stderr,"illegal argument combination\n"); + exit(1); + } + + if ( args.output==NULL ) { + usage(); + fprintf(stderr,"no output specified\n"); + exit(1); + } + + + switch(args.type) { + case trace_ABI: + currSeq = readSeqABI(args.fn); break; + case trace_ALF: + currSeq = readSeqALF(args.fn); break; + case trace_SCF: + currSeq = readSeqSCF(args.fn); break; + default: + usage(); + fprintf(stderr,"Unknown trace file format\n"); + exit(1); + } + + if (currSeq == NULLSeq) { + fprintf(stderr,"Error reading file %s\n",args.fn); + exit(1); + } + + if ( ! writeSeqSCF(currSeq, args.output) ) { + fprintf(stderr,"Error writing file %s\n",args.output); + exit(1); + } + + exit (0); + +} + diff --git a/src/scf/makefile-alpha b/src/scf/makefile-alpha new file mode 100644 index 0000000..331c209 --- /dev/null +++ b/src/scf/makefile-alpha @@ -0,0 +1,67 @@ +# +# getMCH - Alpha version +# + +# +# Programs made by this makefile +# +PROGS = makeSCF + +# +# SWITCHES - see ted makefile +# +#SWITCHES = -DAUTO_CLIP -DDEF_OUT -DSAVE_EDITS + +# +# Where the wild things are +# +SRC = . +TEDSRC = $(SRC)/../ted + +# +# alpha +# +CC= cc +CFLAGS= -g -std1 -ieee_with_inexact $(SWITCHES) -I$(TEDSRC) +LD= cc +LDFLAGS= -g -non_shared +LIBFLAGS = + +%.o : $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + +%.o : $(TEDSRC)/%.c + $(CC) $(CFLAGS) -c $< + +# +# Object files +# +OBJS=\ + makeSCF.o\ + writeSCF.o\ + seq.o\ + seqIOPlain.o\ + seqIOABI.o\ + seqIOEdit.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqOutput.o\ + opp.o\ + fpoint.o\ + match.o\ + mach-io.o + + +# +# Linking +# +makeSCF: $(OBJS) + $(LD) $(LDFLAGS) -o $@ $(OBJS) $(LIBS) $(LIBFLAGS) + +# +# Special dependencies +# +all: $(PROGS) + +clean: + /bin/rm -f $(OBJS) $(PROGS) *~ diff --git a/src/scf/makefile-dec b/src/scf/makefile-dec new file mode 100644 index 0000000..86d61a4 --- /dev/null +++ b/src/scf/makefile-dec @@ -0,0 +1,64 @@ +# +# getMCH - DEC Ultrix (mips) fork +# + +# +# Programs made by this makefile +# +PROGS = makeSCF + +# +# SWITCHES - see ted makefile +# +#SWITCHES = -DAUTO_CLIP -DDEF_OUT -DSAVE_EDITS + +# +# Where the wild things are +# +SRC = . +TEDSRC = $(SRC)/../ted +INCLUDES = -I/usr/include/mit -I$(TEDSRC) +# +# DEC +# +CC= c89 +CFLAGS= -g -std -w0 $(SWITCHES) $(INCLUDES) + +%.o : $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + +%.o : $(TEDSRC)/%.c + $(CC) $(CFLAGS) -c $< + +# +# Object files +# +OBJS=\ + makeSCF.o\ + writeSCF.o\ + seq.o\ + seqIOPlain.o\ + seqIOABI.o\ + seqIOEdit.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqOutput.o\ + opp.o\ + fpoint.o\ + match.o\ + mach-io.o + + +# +# Linking +# +makeSCF: $(OBJS) + $(CC) $(CFLAGS) -o $@ $(OBJS) $(LIBS) + +# +# Special dependencies +# +all: $(PROGS) + +clean: + /bin/rm -f $(OBJS) $(PROGS) *~ diff --git a/src/scf/makefile-sgi b/src/scf/makefile-sgi new file mode 100644 index 0000000..539c9ab --- /dev/null +++ b/src/scf/makefile-sgi @@ -0,0 +1,67 @@ +# +# getMCH - SGI version +# + +SHELL=/bin/sh + +# +# Programs made by this makefile +# +PROGS = makeSCF + +# +# SWITCHES - see ted makefile +# +#SWITCHES = -DAUTO_CLIP -DDEF_OUT -DSAVE_EDITS + +# +# Where the wild things are +# +SRC = . +TEDSRC = $(SRC)/../ted + +# +# Sun +# +CC= cc +CFLAGS= -g -ansi -prototypes $(SWITCHES) -I$(TEDSRC) +LIBFLAGS = + +%.o : $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + +%.o : $(TEDSRC)/%.c + $(CC) $(CFLAGS) -c $< + +# +# Object files +# +OBJS=\ + makeSCF.o\ + writeSCF.o\ + seq.o\ + seqIOPlain.o\ + seqIOABI.o\ + seqIOEdit.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqOutput.o\ + opp.o\ + fpoint.o\ + match.o\ + mach-io.o + + +# +# Linking +# +makeSCF: $(OBJS) + $(CC) $(CFLAGS) -o $@ $(OBJS) $(LIBS) $(LIBFLAGS) + +# +# Special dependencies +# +all: $(PROGS) + +clean: + /bin/rm -f $(OBJS) $(PROGS) *~ diff --git a/src/scf/makefile-solaris b/src/scf/makefile-solaris new file mode 100644 index 0000000..a276972 --- /dev/null +++ b/src/scf/makefile-solaris @@ -0,0 +1,68 @@ +# +# getMCH - SunOS fork +# + +# +# Programs made by this makefile +# +PROGS = makeSCF + +# +# SWITCHES - see ted makefile +# +#SWITCHES = -DAUTO_CLIP -DDEF_OUT -DSAVE_EDITS + +# +# Where the wild things are +# +SRC = . +TEDSRC = $(SRC)/../ted + +# +# Sun +# +CC= gcc +GCCWARNINGS = -Wreturn-type -Wunused -Wswitch -Wcomment -W -Wformat \ + -Wuninitialized +CFLAGS= -g -ansi -pedantic $(GCCWARNINGS) $(SWITCHES) -I$(TEDSRC) -I/usr/openwin/include +# gcc2.1 -static, cc -Bstatic, else nothing +LIBFLAGS = -static + +%.o : $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + +%.o : $(TEDSRC)/%.c + $(CC) $(CFLAGS) -c $< + +# +# Object files +# +OBJS=\ + makeSCF.o\ + writeSCF.o\ + seq.o\ + seqIOPlain.o\ + seqIOABI.o\ + seqIOEdit.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqOutput.o\ + opp.o\ + fpoint.o\ + match.o\ + mach-io.o + + +# +# Linking +# +makeSCF: $(OBJS) + $(CC) $(CFLAGS) -o $@ $(OBJS) $(LIBS) $(LIBFLAGS) + +# +# Special dependencies +# +all: $(PROGS) + +clean: + /bin/rm -f $(OBJS) $(PROGS) *~ diff --git a/src/scf/makefile-sun b/src/scf/makefile-sun new file mode 100644 index 0000000..7d336de --- /dev/null +++ b/src/scf/makefile-sun @@ -0,0 +1,68 @@ +# +# getMCH - SunOS fork +# + +# +# Programs made by this makefile +# +PROGS = makeSCF + +# +# SWITCHES - see ted makefile +# +#SWITCHES = -DAUTO_CLIP -DDEF_OUT -DSAVE_EDITS + +# +# Where the wild things are +# +SRC = . +TEDSRC = $(SRC)/../ted + +# +# Sun +# +CC= gcc +GCCWARNINGS = -Wreturn-type -Wunused -Wswitch -Wcomment -W -Wformat \ + -Wuninitialized +CFLAGS= -g -ansi -pedantic $(GCCWARNINGS) $(SWITCHES) -I$(TEDSRC) +# gcc2.1 -static, cc -Bstatic, else nothing +LIBFLAGS = -static + +%.o : $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + +%.o : $(TEDSRC)/%.c + $(CC) $(CFLAGS) -c $< + +# +# Object files +# +OBJS=\ + makeSCF.o\ + writeSCF.o\ + seq.o\ + seqIOPlain.o\ + seqIOABI.o\ + seqIOEdit.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqOutput.o\ + opp.o\ + fpoint.o\ + match.o\ + mach-io.o + + +# +# Linking +# +makeSCF: $(OBJS) + $(CC) $(CFLAGS) -o $@ $(OBJS) $(LIBS) $(LIBFLAGS) + +# +# Special dependencies +# +all: $(PROGS) + +clean: + /bin/rm -f $(OBJS) $(PROGS) *~ diff --git a/src/scf/match.c b/src/scf/match.c new file mode 100644 index 0000000..6a2534b --- /dev/null +++ b/src/scf/match.c @@ -0,0 +1,62 @@ +int string_match(seq1, n1, seq2, n2, nmiss, indices) + +/* + This function may be called once from initialDisplayedSeq if + the user has input a string they wish to search for in the + input sequence -- this subroutine returns *indices, the first + position at which the query sequence (app_resources.astring) + matched the input file sequence (currSeq). */ + +/* a modified version of match.c + finds alignments between a search sequence, seq1, and a target sequence, + seq2, with no gaps (except possibly at ends) + and at most nmiss mismatches (relative to seq1), + n1 is the length of seq1 where seq1 is assumed to start at indices 0, + n2 is the length of seq2 where seq2 is assumed to start at indices 0 */ + char *seq1, *seq2; + int n1, n2, nmiss; + int *indices; +{ + int i, j, d, istart, iend, i_miss, n_match; + int mtable[100][5]; /* 100 would be, now the total number + of matches the program may find between + a given input string and the sequence */ + + +/* d = j - i is the "offset" between the two sequences */ + if (n1 - n2 > nmiss) return (0); + n_match = 0; + for (d = -nmiss; d <= n2 + nmiss - n1; d++){ + if (d < 0) istart = i_miss = -d; + else istart = i_miss = 0; + if (d > n2 - n1) { + iend = n2 - d; + i_miss += n1 + d - n2; + } + else iend = n1; + for (i = istart, j = d + i; i < iend; i++, j++) + if (seq1[i] != seq2[j] && ++i_miss > nmiss) goto nextd; + mtable[n_match][0] = d + istart; /* indices (assuming they start at + 0) of starting nucleotide in the + searched sequence */ + mtable[n_match][1] = istart; /* nucleotide position in the query + sequence where match starts (assuming + query index starts with 0) */ + mtable[n_match][2] = iend - istart; /* number of nucleotides in the + match */ + if (mtable[n_match][2]==n1) { + indices[n_match] = mtable[n_match][0]; + } + + mtable[n_match][3] = i_miss; /* number of mismatches */ + n_match++; /* number of matches */ + if (n_match >= 100) return(n_match); + + nextd:; + } + + return (n_match); + return(0); /*return 0 if it got to this point; that means + it must have not found an exact match, so + you want the baseNum to be 0 in that case*/ +} diff --git a/src/scf/match.h b/src/scf/match.h new file mode 100644 index 0000000..fc6b802 --- /dev/null +++ b/src/scf/match.h @@ -0,0 +1,19 @@ +#ifndef _match_h +#define _match_h + +/* + Title: match + + File: match.h + Purpose: match + Last update: Thu Jan 03 1991 +*/ + + + + +/* ---- Includes ---- */ + +extern int string_match(); + +#endif _match_h diff --git a/src/scf/writeSCF.c b/src/scf/writeSCF.c new file mode 100644 index 0000000..81d807a --- /dev/null +++ b/src/scf/writeSCF.c @@ -0,0 +1,255 @@ +/* + Title: writeSCF + + File: writeSCF.c + Purpose: Output of Standard Chromatogram Format sequences + Last update: 23 November 1992 + + Change log: + 4 Feb 1992, Now draft proposal version 2 + 23 Nov 92, SCF 2.0 + LaDeana's changes + +*/ + + +/* +** makeSCF version number +*/ +#define VERSION_NO "2.00" + +/* ---- Imports ---- */ + + +#include "scf.h" /* IMPORT: scf structures */ +#include "seq.h" /* IMPORT: Seq, BasesAndTraces, NULLSeq, + newSeq, freeSeq */ + + +#include "mach-io.h" /* IMPORT: be_write_int_1, be_write_int_2, be_write_int_4 */ + +#include +#include /* IMPORT: fopen, fclose, fseek, ftell, fgetc, + EOF */ + + + + + + + +/* ---- Internal functions ---- */ + + + + +static int write_scf_header(FILE *fp, Header *h) +{ + int i; + + if (be_write_int_4(fp,&h->magic_number)==False) return (False); + if (be_write_int_4(fp,&h->samples)==False) return (False); + if (be_write_int_4(fp,&h->samples_offset)==False) return (False); + if (be_write_int_4(fp,&h->bases)==False) return (False); + if (be_write_int_4(fp,&h->bases_left_clip)==False) return (False); + if (be_write_int_4(fp,&h->bases_right_clip)==False) return (False); + if (be_write_int_4(fp,&h->bases_offset)==False) return (False); + if (be_write_int_4(fp,&h->comments_size)==False) return (False); + if (be_write_int_4(fp,&h->comments_offset)==False) return (False); + if (fwrite(h->version,sizeof(h->version),1,fp)!=1) return (False); + if (be_write_int_4(fp,&h->sample_size)==False) return (False); + if (be_write_int_4(fp,&h->code_set)==False) return (False); + for (i=0;i<20;i++) + if (be_write_int_4(fp,&h->spare[i])==False) return (False); + + return (True); +} + + +static int write_scf_sample1(FILE *fp, Samples1 *s) +{ + if (be_write_int_1(fp,&s->sample_A)==False) return (False); + if (be_write_int_1(fp,&s->sample_C)==False) return (False); + if (be_write_int_1(fp,&s->sample_G)==False) return (False); + if (be_write_int_1(fp,&s->sample_T)==False) return (False); + + return (True); +} + + +static int write_scf_sample2(FILE *fp, Samples2 *s) +{ + if (be_write_int_2(fp,&s->sample_A)==False) return (False); + if (be_write_int_2(fp,&s->sample_C)==False) return (False); + if (be_write_int_2(fp,&s->sample_G)==False) return (False); + if (be_write_int_2(fp,&s->sample_T)==False) return (False); + + return (True); +} + + + + + +static int write_scf_base(FILE *fp, Bases *b) +{ + if (be_write_int_4(fp,&b->peak_index)==False) return (False); + if (be_write_int_1(fp,&b->prob_A)==False) return (False); + if (be_write_int_1(fp,&b->prob_C)==False) return (False); + if (be_write_int_1(fp,&b->prob_G)==False) return (False); + if (be_write_int_1(fp,&b->prob_T)==False) return (False); + if (be_write_int_1(fp,(uint_1 *)&b->base)==False) return (False); + if (be_write_int_1(fp,&b->spare[0])==False) return (False); + if (be_write_int_1(fp,&b->spare[1])==False) return (False); + if (be_write_int_1(fp,&b->spare[2])==False) return (False); + + return (True); +} + + + +static int write_scf_comment(FILE *fp, Comments *c, size_t l) +{ + if (fwrite(c, l, 1, fp) !=1) return (False); + return (True); + +} + + + + +/* ---- Exports ---- */ + + + + + + + + +Boolean writeSeqSCF(Seq seq, char *fn) +/* +** Write Seq out as a .scf file +*/ +{ + + FILE *fp; + Header header; + Bases base; + Comments comments[1024]; + Comments default_comments[] = "conversion_program = makeSCF " VERSION_NO "\n"; + char *src; + + + if ((fp = fopen(fn,"wb"))==NULL) + return (False); + else { + int i; + int prec; /* precision to use */ + + /* source dependant switches */ + prec = 1; + switch (seq->format) { + case ABIFormat: + src = "ABI 373A"; + break; + case ALFFormat: + src = "Pharmacia A.L.F."; + prec = 2; + break; + case SCFFormat: + src = "SCF"; + if (seq->maxTraceVal > 255) prec=2; /* retain precision */ + break; + default: + src = "Unknown"; + break; + } + + if (seq->info==NULL) { + sprintf(comments,"%ssource = %s\n", + default_comments, + src); + } else { + sprintf(comments,"%s\n%ssource = %s\n", + seq->info, + default_comments, + src); + } + + header.magic_number = SCF_MAGIC; + header.samples = seq->NPoints; + header.samples_offset = (uint_4)sizeof(Header); + header.bases = seq->NorigBases; + header.bases_left_clip = seq->leftCutoff; + header.bases_right_clip = seq->rightCutoff; + header.bases_offset = (uint_4)(header.samples_offset + header.samples * ((prec==2)?sizeof(Samples2):sizeof(Samples1))); + header.comments_size = (uint_4)strlen(comments)+1; + header.comments_offset = (uint_4)(header.bases_offset + header.bases * sizeof(Bases)); + strncpy(header.version, VERSION_NO,4); + header.sample_size = prec; + header.code_set = CSET_DEFAULT; + + for(i=0;i<20;i++) header.spare[i]=0; + if (write_scf_header(fp, &header)==False) return (False); + + + switch(prec) { + case 2: + { + Samples2 sample; + + for(i=0; itraceA[i]; + sample.sample_C = seq->traceC[i]; + sample.sample_G = seq->traceG[i]; + sample.sample_T = seq->traceT[i]; + if (write_scf_sample2(fp, &sample)==False) return(False); + } + break; + } + default: + { + Samples1 sample; + + for(i=0; itraceA[i],seq->maxTraceVal); + sample.sample_C = scale(seq->traceC[i],seq->maxTraceVal); + sample.sample_G = scale(seq->traceG[i],seq->maxTraceVal); + sample.sample_T = scale(seq->traceT[i],seq->maxTraceVal); + if (write_scf_sample1(fp, &sample)==False) return(False); + } + break; + } + } + + + for(i=0; ibasePos[i]; + base.base = seq->base[i]; + base.spare[0] = base.spare[1] = base.spare[2] = 0; + base.prob_A = base.prob_C = base.prob_G = base.prob_T = 0; + switch(base.base) { + case 'A' : case 'a': + base.prob_A = 1; break; + case 'C' : case 'c': + base.prob_C = 1; break; + case 'G' : case 'g': + base.prob_G = 1; break; + case 'T' : case 't': + base.prob_A = 1; break; + default: + base.prob_A = base.prob_C = base.prob_G = base.prob_T = 1; + } + if (write_scf_base(fp,&base)==False) return (False); + + } + + if (write_scf_comment(fp,comments,(size_t)header.comments_size)==False) return (False); + + + fclose(fp); + } + + return (True); +} diff --git a/src/scf/writeSCF.h b/src/scf/writeSCF.h new file mode 100644 index 0000000..5c09613 --- /dev/null +++ b/src/scf/writeSCF.h @@ -0,0 +1,10 @@ +#ifndef _writeSCF_h +#define _writeSCF_h + +#include "scf.h" /* IMPORT: scf structures */ +#include "seq.h" /* IMPORT: Seq, BasesAndTraces, NULLSeq, + newSeq, freeSeq */ + +extern Boolean writeSeqSCF(Seq seq, char *fn); + +#endif /* _writeSCF_h */ diff --git a/src/scripts/FE-stealdata b/src/scripts/FE-stealdata new file mode 100644 index 0000000..08397bc --- /dev/null +++ b/src/scripts/FE-stealdata @@ -0,0 +1,51 @@ +#! /bin/csh -f +# +# stealdata (front end) +# +# Borrow data and files from a neighbouring cosmid database +# +# +# Change log: +# +# v1.8 Front and back end version +# + +onintr abort + +# +# set defaults here +# +# NOTE: "other" refers to the project you want to pinch data off +# NOTE: db_type and other_db_type must be either "dap" or "bap" +# +set project = F54G8 +set version = 0 +set db_type = dap +set other_dir = ~kt/ZC84 +set other_project = ZC84 +set other_version = 0 +set other_db_type = bap + +# +# available operations are: +# r = report useful readings (response mailed) +# s = "r" + copy useful sequences only +# a = "r" + copy useful sequences AND traces +# l = "r" + copy useful sequences and LINK traces +# default = "r" +# +set operation = "s" + + + +#**************************************************** +# MAKE NO CHANGES BELOW THIS LINE +#**************************************************** +FE-stealdata.csh $project $version $db_type $other_project $other_dir $other_version $other_db_type $operation +if ( $status ) then +abort: + echo stealdata abnormally terminated + exit 1 +endif + +exit 0 diff --git a/src/scripts/FE-stealdata.csh b/src/scripts/FE-stealdata.csh new file mode 100644 index 0000000..5115d06 --- /dev/null +++ b/src/scripts/FE-stealdata.csh @@ -0,0 +1,380 @@ +#! /bin/csh -f +# +# stealdata.csh +# +# Borrow data and files from a neighbouring cosmid database +# +# +# Change log: +# +# v1.2 Handle dap and bap databases +# v1.3 Provide linking option +# v1.4 Under no circumstances link sequence files +# v1.5 Bug in option "l". Now works when databases have same name +# v1.6 To work with new version of bap +# v1.7 Variable report now set correctly +# v1.8 Front and back end version +# + +onintr abort + +if ( $#argv != 8 ) then + echo Usage: stealdata.csh ... (8 args) + exit 1 +endif + +set project = $1 +set version = $2 +set db_type = $3 +set other_dir = $4 +set other_project = $5 +set other_version = $6 +set other_db_type = $7 +set operation = $8 + + +# +# other - DON'T TOUCH +# +set trace_suffixes = ALF,RES,SCF + +# +# check defaults +# +if (! -e ${project}.SQ${version}) then + echo Project ${project} version ${version} does not exist + exit 1 +endif + +if (! -d ${other_dir}) then + echo Directory ${other_dir} does not exist + exit 1 +endif + +if (! -e ${other_dir}/${other_project}.SQ${other_version}) then + echo Project ${other_dir}/${other_project} version ${other_version} does not exist + exit 1 +endif + +# +# Report file +# +set report = $cwd/_steal.rep.${project} +set count = 0 +while ( -e $report ) + # echo Report file ${report} already exists... + @ count ++ + set report = $cwd/_steal.rep.${project}.${count} +end + +touch ${report} + +# +# Output details of stealdata run +# +cat << EOF | tee -a ${report} +Stealdata v1.6 +Borrow data from neighbouring cosmid database + +project = ${project} +version = ${version} +db_type = ${db_type} +other_dir = ${other_dir} +other_project = ${other_project} +other_version = ${other_version} +other_db_type = ${other_db_type} +operation = ${operation} +EOF + +switch ( ${operation} ) +case "r": + echo " ( report only )" | tee -a ${report} + breaksw +case "s": + echo " ( copy sequences only, report )" | tee -a ${report} + breaksw +case "a": + echo " ( copy sequences and traces, report )" | tee -a ${report} + breaksw +case "l": + echo " ( copy sequences and link traces, report )" | tee -a ${report} + breaksw +default: + echo " ( unknown - report only )" | tee -a ${report} + set operation = "r" + breaksw +endsw +echo "" | tee -a ${report} + +# +# create a temporary directory +# +echo Creating a temporary directory | tee -a ${report} +set temp_dir = _steal.dir.${other_project} +set count = 0 +while (-e ${temp_dir}) + echo Temporary directory ${temp_dir} already exists | tee -a ${report} + @ count ++ + set temp_dir = _steal.dir.${other_project}.${count} +end + +set home_dir = `pwd` +mkdir ${temp_dir} + +# +#consensus for database +# +set consensus = _steal.seq.${project} +set count = 0 +while ( -e $consensus ) + echo Consensus file ${consensus} already exists | tee -a ${report} + @ count ++ + set consensus = _steal.seq.${project}.${count} +end + +# +# determine consensus +# +echo Determining the consensus for project ${project} version ${version} | tee -a ${report} +# +# FUDGE!!! +# The third question in option 8 (Calculate consensus) +# has changed in the latest version of bap. +# Was: +# ? Make another consensus (y/n) (y) +# Now is: +# ? Staden format (y/n) (y) = +# +# Set fudge to reflect the appropriate choice +# +if ( ${db_type} == "bap" ) then + set fudge = y +else + set fudge = n +endif +${db_type} << EOF > /dev/null + +y +${project} +${version} + +8 +${consensus} +y +${fudge} + +! +EOF +if ( $status ) then + echo stealdata: failed to make consensus | tee -a ${report} + goto abort +endif + + +# +# move consensus to temp_dir +# +mv ${consensus} ${temp_dir} +if ( $status ) then + echo stealdata: failed to move consensus to temp_dir | tee -a ${report} + goto abort +endif + + +# +# copy other database to this directory +# +echo Copying project ${other_project} version ${other_version} | tee -a ${report} +cd ${temp_dir} +cp -i \ + ${other_dir}/${other_project}.SQ${other_version} \ + ${other_dir}/${other_project}.AR${other_version} \ + ${other_dir}/${other_project}.RL${other_version} \ + ${other_dir}/${other_project}.TG${other_version} \ + ${other_dir}/${other_project}.CC${other_version} \ + . +if ( $status ) then + echo stealdata: failed to copy other database | tee -a ${report} + goto abort +endif + + +# +# file of file names in other database +# +set other_files = ${other_project}.all +set other_useless = ${other_project}.useless +set other_useful = ${other_project}.useful +set other_new = ${other_project}.fn.$$ + +# +# extract gel readings +# +set option_extract = 32 +# +# screen against vector +# +set option_screen = 18 +# +echo Extracting gel readings from ${other_project} and | tee -a ${report} +echo " screening against consensus from ${project}" | tee -a ${report} +${other_db_type} << EOF > /dev/null + +y +${other_project} +${other_version} + +${option_extract} +${other_files} +n + +${option_screen} +y +${other_files} +${other_useless} +${consensus} +30 + +! +EOF +if ( $status ) then + echo stealdata: failed to extact or screen readings | tee -a ${report} + goto abort +endif + +# +# determine useful files in other project +# +echo Determining useful gel readings | tee -a ${report} +sort ${other_files} | tr -d ' ' > ${other_files}.nice +sort ${other_useless} | tr -d ' ' > ${other_useless}.nice +comm -23 ${other_files}.nice ${other_useless}.nice > ${other_useful} + +# +# create report header +# +echo "" | tee -a ${report} +echo Useful gel readings: | tee -a ${report} + +# +# determine new files +# +touch ${other_new} +set useful = 0 +foreach x ( `cat ${other_useful}` ) + if ( -e ../$x ) then + echo $x | tee -a ${report} + else + echo $x "- NEW" | tee -a ${report} + echo $x >> ${other_new} + @ useful ++ + endif +end + +if ( $useful == 0 ) then + echo No new gel readings | tee -a ${report} + goto done +endif + +# +# transfer sequences +# +if (${operation} == "s") then + echo "" | tee -a ${report} + echo "Transfering sequence files: " | tee -a ${report} + foreach x ( `cat ${other_new}` ) + cp -i ${other_dir}/${x} .. + echo ${other_dir}/${x} | tee -a ${report} + end + # file of file names + mv ${other_new} .. + echo "" | tee -a ${report} + echo File of file names is ${other_new} | tee -a ${report} +endif + +# +# transfer sequences and traces +# +if (${operation} == "a") then + echo "" | tee -a ${report} + echo "Transfering sequence and trace files: " | tee -a ${report} + foreach x ( `cat ${other_new}` ) + # copy sequence file + cp -i ${other_dir}/${x} .. + echo ${other_dir}/${x} | tee -a ${report} + foreach file ( ${other_dir}/${x}{${trace_suffixes}} ) + if (-e $file) then + cp -i $file .. + echo $file | tee -a ${report} + endif + end + end + # file of file names + mv ${other_new} .. + echo "" | tee -a ${report} + echo File of file names is ${other_new} | tee -a ${report} +endif + +# +# transfer sequences and link traces +# +if (${operation} == "l") then + echo "" | tee -a ${report} + echo "Transfering sequence and trace files: " | tee -a ${report} + set files = `cat ${other_new}` + pushd .. >& /dev/null + foreach x ( $files ) + # copy sequence file + cp -i ${other_dir}/${x} . + echo ${other_dir}/${x} | tee -a ${report} + foreach file ( ${other_dir}/${x}{${trace_suffixes}} ) + # link to traces + if (-e $file) then + ln -s $file + echo $file | tee -a ${report} + endif + end + end + popd >& /dev/null + # file of file names + mv ${other_new} .. + echo "" | tee -a ${report} + echo File of file names is ${other_new} | tee -a ${report} +endif + +done: +# +# cleanup +# +echo Cleaning up | tee -a ${report} +cd .. +/bin/rm -r ${temp_dir} + +# +# Finish up +# +echo "" | tee -a ${report} +echo "Completed" | tee -a ${report} + +# +# mail report +# +echo Mailing you a report +mail `whoami` < ${report} + + +exit 0 + + +# +# +# +abort: + echo stealdata aborted `date` | tee -a ${report} + if ( $?home_dir ) then + cd $home_dir + echo Cleaning up | tee -a ${report} + /bin/rm -r ${temp_dir} + endif + + exit 1 diff --git a/src/scripts/assemble b/src/scripts/assemble new file mode 100644 index 0000000..770c286 --- /dev/null +++ b/src/scripts/assemble @@ -0,0 +1,168 @@ +# +# IMPORTANT NOTICE: +# Assemble will only work with bap version 12.1 or greater +# +# Script to assemble data into a database using a file of file names. +# The batch of readings is screened against two vectors. Any matching +# the vectors have their names added to growing files of vector failures. +# Those that pass are assembled. Those that fail assembly have their names +# added to a growing list of failures. Output from +# the initial assembly is directed to a file that is deleted, hence +# nothing appears on the screen. The failed files are then recompared +# with the output directed to another file. This file is printed then +# both output files are deleted. The procedure starts by making a copy +# of the database to copy X (preceded by its deletion!!!!). +# +# use: assemble fileoffilenames +# +unset noclobber +onintr bailout +set PROJECT = F02A9 +set VECTOR_SCORE = 20 +set ASSEMBLY_SCORE = 20 +set MAX_READ_PADS = 25 +set MAX_CONTIG_PADS = 25 +set MAX_MISMATCH_1 = 8 +set MAX_MISMATCH_2 = 15 +if ($#argv != 1) then +echo "Usage: assemble fileoffilenames" + exit 1 +endif +if (! -f $1) then +echo "File of file names not found" + exit 2 +endif +if (! -f `head -1 $1`) then +echo "First file in file of file names not found" + exit 3 +endif +echo -n "Assembling data from file of filenames "; echo -n $1 +echo -n " into data base "; echo $PROJECT +echo -n "Deleting database backup: "; echo -n $PROJECT +echo " version X" +\rm -f $PROJECT.RLX +\rm -f $PROJECT.SQX +\rm -f $PROJECT.ARX +\rm -f $PROJECT.CCX +\rm -f $PROJECT.TGX +bap <> $PROJECT.FAILS +echo -n "Assembly for project ">>show1$$ +echo -n $PROJECT >>show1$$ +echo -n " on " >>show1$$ +date >> show1$$ +echo -n "In directory " >> show1$$ +pwd >> show1$$ +echo -n "Input file of file names ">>show1$$ +echo $1 >> show1$$ +echo "Parameters used" >>show1$$ +echo -n "Minimum initial match " >>show1$$ +echo $ASSEMBLY_SCORE >> show1$$ +echo -n "Maximum pads in each reading " >>show1$$ +echo $MAX_READ_PADS >> show1$$ +echo -n "Maximum pads in contig " >>show1$$ +echo $MAX_CONTIG_PADS >> show1$$ +echo -n "Maximum percent mismatch (for assembly) " >>show1$$ +echo $MAX_MISMATCH_1 >> show1$$ +echo -n "Maximum percent mismatch (for report) " >>show1$$ +echo $MAX_MISMATCH_2 >> show1$$ +echo "List of m13mp18 failures">>show1$$ +cat $1 | tr -d " " | sort >! sortin$$ +cat passm13$$ | tr -d " " | sort >! sortm13$$ +comm -23 sortin$$ sortm13$$ >> show1$$ +echo "List of bluescribe failures">>show1$$ +#assuming already have sortm13$$ +cat passblue$$ | tr -d " " | sort >! sortblue$$ +comm -23 sortm13$$ sortblue$$ >> show1$$ +comm -23 sortin$$ sortm13$$ >> $PROJECT.M13 +comm -23 sortm13$$ sortblue$$ >> $PROJECT.BLUE +echo "List of entry failures ">>show1$$ +cat fail2$$ >> show1$$ +#cp fail$$ show1$$ +echo "Alignments for entry failures ">>show1$$ +cat show$$ >> show1$$ +lpr show1$$ +bailout: +\rm fail$$ +\rm fail2$$ +\rm -f out$$ +\rm -f show$$ +\rm -f show1$$ +\rm -f passm13$$ +\rm -f passblue$$ +\rm -f sortin$$ +\rm -f sortm13$$ +\rm -f sortblue$$ +#cat $PROJECT.FAILS +; + diff --git a/src/scripts/clip-seq.awk b/src/scripts/clip-seq.awk new file mode 100644 index 0000000..c938a58 --- /dev/null +++ b/src/scripts/clip-seq.awk @@ -0,0 +1,173 @@ +# +# File: clip-seq.awk +# Version: 1.0 +# Created: 17 November 1992 +# +# Author: Simon Dear +# MRC Laboratory of Molecular Biology +# Hills Road +# Cambridge CB2 2QH +# United Kingdom +# +# Description: +# +# Clip poor quality sequence at the left (5') and right (3') ends +# on basis of sequence content. Program acts as a filter for xdap +# sequence files. +# +# Rule: +# +# At the 3' end: A window of (MM) bases is slid down the sequence, +# starting from base position (SR) until there are (NN) Ns within the +# window, or until the window reaches position (RR), whichever happens +# first. The 3' extent of the good data is the set to be (KR) bases +# upsteam of this position. +# +# At the 5' end: This essentially the same as for the 3' end. The window +# is slid back along the sequence starting at base position (SL), until +# there are sufficient Ns or until the window reaches position (LL), +# whichever happens first. There is no further adjustment of the 5' +# extent of good data. +# +# St.Louis use: NN=2, MM=5, SL=1, LL=1, SR=200, RR=sequence_length, KR=0 +# Cambridge use: NN=2, MM=5, SL=100, LL=1, SR=200, RR=450, KR=50 +# + +BEGIN { + # Up to where NN uncertainties in window of MM bases + NN = 2; + MM = 5; + SL = 100 + SR = 200 + LL = 1; + RR = 450; + KR = 50; + # don't change + QL = -1; + QR = -1; +} + + +/;/ && NR==1 { header = $0; next; } #first line is a header +/;/ { rseq = rseq substr($0,3); next; } +/;/ { if (extras=="") extras = $0; else extras = extras "\n" $0; next; } # gather extras + { mseq = mseq $0; next; } + +END { + seq = lseq mseq rseq; + # fudge QL and QR + QL = length(lseq); + QR = length(lseq) + length(mseq) + 1; + + + seqlen = length(seq); + if (RR > seqlen) RR = seqlen; + if (LL < 1) LL = 1 + lcut = LL - 1; + rcut = RR+1; + if ( NN>0 ) { + # + # clip to the right + # + score = 0; + # calculate default score + for(j=SR-1;j0 && index("ACGTacgt",substr(seq,j,1)) == 0) + score++; + # loop through + for(j=SR; j<=RR; j++) { + if (j>1 && index("ACGTacgt",substr(seq,j-1,1)) == 0) + score--; + if (j+MM-1<=seqlen && index("ACGTacgt",substr(seq,j+MM-1,1)) == 0) + score++; + if (score >= NN) { + rcut = j; + break; + } + } + + # + # clip to the left + # + score = 0; + # calculate default score + for(j=SL+1;j>SL-MM+1 && j>0 ; j--) + if(j<=seqlen && index("ACGTacgt",substr(seq,j,1)) == 0) + score++; + # loop through + for(j=SL; j>=LL; j--) { + if (j0 && index("ACGTacgt",substr(seq,j-MM+1,1)) == 0) + score++; + if (score >= NN) { + lcut = j; + break; + } + } + } + + # knock back the 3' end + rcut = rcut - KR; + if (rcut <= lcut) rcut = lcut + 1; + + # Determine old values + if (QR<0) QR = seqlen+1; + if (QL<0) QL = 0; + # Constrain new values to old values + if (lcut >= QR) lcut = QR - 1; + if (rcut <= QL) rcut = QL + 1; + # Adjust if there are changes + if (lcut < QL) lcut = QL; + if (rcut > QR) rcut = QR; + + # + # Print out the filtered file + # + + # Write dap file header + if (length(header)==0) { + printf(";%6d%6d%6d%-4s%-18s\n",seqlen,lcut,rcut-lcut-1,"PLN",""); + } else { + printf(";%6d%6d%6d%s\n",seqlen,lcut,rcut-lcut-1,substr(header,20)); + } + + + + # Write left cutoffs (if any) + if(lcut>0) { + str = substr(seq,1,lcut); + strlen = lcut; + bits = 48; + for (i = 1; i <= strlen; i+=bits) { + printf (";<%s\n",substr(str,i,bits)); + } + } + + # Write right cutoffs (if any) + if(rcut<=seqlen) { + str = substr(seq,rcut); + strlen = length(str); + bits = 48; + for (i = 1; i <= strlen; i+=bits) { + printf (";>%s\n",substr(str,i,bits)); + } + } + + # Write extras here + if (extras!="") print extras; + + + # Write remaining sequence (if any!) + if (lcut+1 != rcut) { + str = substr(seq,lcut+1,rcut-lcut-1); + strlen = length(str); + bits = 50; + for (i = 1; i <= strlen; i+=bits) { + printf ("%s\n",substr(str,i,bits)); + } + } + + +} diff --git a/src/scripts/clip-seqs b/src/scripts/clip-seqs new file mode 100644 index 0000000..329e10d --- /dev/null +++ b/src/scripts/clip-seqs @@ -0,0 +1,42 @@ +#!/bin/csh -f +# +# clip-seqs +# +# clip all sequences in a file of file names on quality +# +# Usage: +# clip-seqs fof +# +onintr abort + +if ( $#argv != 1 ) then + echo Usage: clip-seqs fof + exit 2 +endif + +if (! -f "$1") then + echo clip-seqs: file "$1" does not exist + exit 1 +endif + + +foreach file ( `cat "$1"`) + /bin/rm -f tmp.$$ + awk -f clip-seq.awk $file > tmp.$$ + if ( $status ) then + echo clip-seqs: error while clipping $file + goto abort + endif + /bin/mv $file ${file}~ + /bin/mv tmp.$$ $file +end + + + +exit 0 + +abort: + +echo aborted `date` +/bin/rm -f tmp.$$ +exit 1 \ No newline at end of file diff --git a/src/scripts/clipping.doc b/src/scripts/clipping.doc new file mode 100644 index 0000000..e289b90 --- /dev/null +++ b/src/scripts/clipping.doc @@ -0,0 +1,37 @@ +Marking regions of poor quality Simon Dear Nov 17 1992 + +Regions of poor quality can be excised using the "clip-seqs" script. +This script takes a file of sequence file names as input, and filters +each to the awk program clip-seq.awk. The sequence files are assumed +to be in staden xdap format. For details on this format, see the +README file in $STADENROOT. The files are modified to reflect the +removal of poor data, while the original sequence is retained in a "~" +suffixed file. + +Usage: + clip-seqs file-of-file-names + + +Quality clipping is based on a simple analysis of the base content. By +default, it works as follows. The original pre-clipped sequence is +determined from the sequence file. The extents of good quality +sequence is determined for both 5' and 3' ends of the sequence. The +numbers in brackets are set in clip-seq.awk and can be set to suit +local preferences. + +At the 3' end: A window of (5) bases is slid down the sequence, +starting from base position (200) until there are (2) Ns within the +window, or until the window reaches position (450), whichever happens +first. The 3' extent of the good data is the set to be (50) bases +upsteam of this position. + +At the 5' end: This essentially the same as for the 3' end. The window +is slid back along the sequence starting at base position (100), until +there are sufficient Ns or until the window reaches position (1), +whichever happens first. There is no further adjustment of the 5' +extent of good data. + +If there are existing extents in the sequence file, and they are more +conservative than the ones calculated from the sequence, then they +will be the extents used. + \ No newline at end of file diff --git a/src/scripts/dub b/src/scripts/dub new file mode 100644 index 0000000..3b8cfab --- /dev/null +++ b/src/scripts/dub @@ -0,0 +1,93 @@ +#!/bin/sh + +# looks for any block of 10 which has more than 6 bases which have +# yet to be double stranded + +case $# in + 1) ;; + *)echo dub reads an output file created by the Examine quality; +echo option inside of xdap and outputs a list of which portions of; +echo the sequence have yet to be double stranded and on which strand; +echo 'USAGE: dub examine_quality_output_file ' 1>&2; exit 2;; +esac + + +awk 'BEGIN{start_file=0; start_num=0; prev1=0; prev2=0; prev3=0; prev4=0; + totnum12=0; totnum34=0; totnum1=0; totnum2=0; prevprev1=0; prevprev2=0; printf("REGIONS YET TO BE DOUBLE STRANDED FOR: %s\n\n","'$1'");} +{ + +# right now Bob does not care about information about type 3 and 4 + + +# look for a number 10 to indicate the beginning of quality information + if (NF>0) { +# if column 1 has 10 characters in it then this is +# most likely a line with quality information in it + if (length($1)>8) { +# go through each block of 10 counting the number of problems for +# each row of quality counts + for (j=1; j<=NF; j++) { + prevprev1=prev1; prevprev2=prev2; + prev1=num1; prev2=num2; prev3=num3; prev4=num4; +# num12 and num34 are counts for each block of 10 so reset them +# each time + num1=0;num2=0;num3=0;num4=0;num0=0; + for (i=0; i<=length($j); i++) { +# for codes 1 and 2 you need the other strand + if (substr($j,i,1)=="1") num1++; + if (substr($j,i,1)=="2") num2++; +# for codes 3 and 4 you need both strands to resolve the disagreement +# if (substr($j,i,1)=="3" || substr($j,i,1)=="4") num34++; + } +#if any block of 10 has more than 6 total non-zeros then it is +#a problem area + if (num1+num2>=6) { +# only reset start_num if you have not already started a region of +# problem areas + end_num=count+10*(j-1); + if (start_num==0) { + start_num=count+10*(j-1)-9; +# if that previous block before a problem area had more then 4 problems then go +# ahead and move the start point to the beginning of that previous block + if (prev1+prev2>=4) start_num-=10; + if (prevprev1+prevprev2>=4) start_num-=10; + } + else { + totnum12+=num1+num2; +# totnum34+=num34; + totnum1+=num1; + totnum2+=num2; + } + + } + else { +# if the 3s and 4s make up more than half of the problems then tell the +# user they need to pick up both strands + + if (end_num!=0 && start_num!=0) { +#use totnum1+totnum0 here becuase if it is mostly 0s and 1s then +#you want to just see the plus strand you do not need it to tell +#you both strands +# if (totnum1_totnum0>=(end_num-start_num)/2) printf("Needs plus strand from: %10d to %10d\n",start_num,end_num); +# else if (totnum2+totnum0>(end_num-start_num)/2) printf("Needs minus strand from: %10d to %10d\n",start_num,end_num); + if (totnum1>totnum2) printf("Needs plus strand from: %10d to %10d\n",start_num,end_num); + else if (totnum2>totnum1) printf("Needs minus strand from: %10d to %10d\n",start_num,end_num); + else if (totnum12>6) + printf("Needs one strand from: %10d to %10d\n",start_num,end_num); + } + start_num=0; + end_num=0; + totnum0=0; + totnum1=0; + totnum2=0; + totnum12=0; + totnum34=0; + } + } + } + else + count=$1; +# count is the sequence indices indicator + } +} +END{}' <$1 | sort +1 diff --git a/src/scripts/stealdata b/src/scripts/stealdata new file mode 100644 index 0000000..ce9897a --- /dev/null +++ b/src/scripts/stealdata @@ -0,0 +1,415 @@ +#! /bin/csh -f +# +# stealdata +# +# Borrow data and files from a neighbouring cosmid database +# +# +# Change log: +# +# v1.2 Handle dap and bap databases +# v1.3 Provide linking option +# v1.4 Under no circumstances link sequence files +# v1.5 Bug in option "l". Now works when databases have same name +# v1.6 To work with new version of bap +# v1.7 Variable report now set correctly +# v1.8 Check for BUSY file +# v1.9 There was a bug in the consensus output format fudge +# v1.10 There is a new style dialogue for choosing between +# Staden and Fasta format of outout (26-Jan-92) +# + +onintr abort + +# +# set defaults here +# +# NOTE: "other" refers to the project you want to pinch data off +# NOTE: db_type and other_db_type must be either "dap" or "bap" +# +set project = F54G8 +set version = 0 +set db_type = dap +set other_dir = ~kt/ZC84 +set other_project = ZC84 +set other_version = 0 +set other_db_type = bap + +# +# available operations are: +# r = report useful readings (response mailed) +# s = "r" + copy useful sequences only +# a = "r" + copy useful sequences AND traces +# l = "r" + copy useful sequences and LINK traces +# default = "r" +# +set operation = "a" + +# +# other - DON'T TOUCH +# +set trace_suffixes = ALF,RES,SCF + +# +# check defaults +# +if (! -e ${project}.SQ${version}) then + echo Project ${project} version ${version} does not exist + exit 1 +endif + +if (! -d ${other_dir}) then + echo Directory ${other_dir} does not exist + exit 1 +endif + +if (! -e ${other_dir}/${other_project}.SQ${other_version}) then + echo Project ${other_dir}/${other_project} version ${other_version} does not exist + exit 1 +endif + +# +# Check for BUSY files +# +if ( -e ${project}_BUSY${version} ) then + echo Project ${project} version ${version} is busy - try again later + exit 1 +endif + +# +# Report file +# +set report = $cwd/_steal.rep.${project} +set count = 0 +while ( -e $report ) + # echo Report file ${report} already exists... + @ count ++ + set report = $cwd/_steal.rep.${project}.${count} +end + +touch ${report} + +# +# Output details of stealdata run +# +cat << EOF | tee -a ${report} +Stealdata v1.10 +Borrow data from neighbouring cosmid database + +project = ${project} +version = ${version} +db_type = ${db_type} +other_dir = ${other_dir} +other_project = ${other_project} +other_version = ${other_version} +other_db_type = ${other_db_type} +operation = ${operation} +EOF + +switch ( ${operation} ) +case "r": + echo " ( report only )" | tee -a ${report} + breaksw +case "s": + echo " ( copy sequences only, report )" | tee -a ${report} + breaksw +case "a": + echo " ( copy sequences and traces, report )" | tee -a ${report} + breaksw +case "l": + echo " ( copy sequences and link traces, report )" | tee -a ${report} + breaksw +default: + echo " ( unknown - report only )" | tee -a ${report} + set operation = "r" + breaksw +endsw +echo "" | tee -a ${report} + +# +# create a temporary directory +# +echo Creating a temporary directory | tee -a ${report} +set temp_dir = _steal.dir.${other_project} +set count = 0 +while (-e ${temp_dir}) + echo Temporary directory ${temp_dir} already exists | tee -a ${report} + @ count ++ + set temp_dir = _steal.dir.${other_project}.${count} +end + +set home_dir = `pwd` +mkdir ${temp_dir} + +# +#consensus for database +# +set consensus = _steal.seq.${project} +set count = 0 +while ( -e $consensus ) + echo Consensus file ${consensus} already exists | tee -a ${report} + @ count ++ + set consensus = _steal.seq.${project}.${count} +end + +# +# determine consensus +# +echo Determining the consensus for project ${project} version ${version} | tee -a ${report} +# +# FUDGE!!! +# The third question in option 8 (Calculate consensus) +# has changed in the latest version of bap. +# Was: +# ? Make another consensus (y/n) (y) +# ## Now is: +# ## ? Staden format (y/n) (y) = +# Now is: +# Select output format +# X 1 Staden +# 2 Fasta +# ? Selection (1-2) (1) = +# +# Set fudge to reflect the appropriate choice +# +if ( ${project} == "bap" ) then + set fudge = "" + # set fudge = y +else + set fudge = n +endif + +# +# One last minute check for BUSY files +# +if ( -e ${project}_BUSY${version} ) then + echo Project ${project} version ${version} is busy - try again later + goto abort +endif + +${db_type} << EOF > /dev/null + +y +${project} +${version} + +8 +${consensus} +y +${fudge} + +! +EOF +if ( $status ) then + echo stealdata: failed to make consensus | tee -a ${report} + goto abort +endif + + +# +# move consensus to temp_dir +# +mv ${consensus} ${temp_dir} +if ( $status ) then + echo stealdata: failed to move consensus to temp_dir | tee -a ${report} + goto abort +endif + + +# +# copy other database to this directory +# +echo Copying project ${other_project} version ${other_version} | tee -a ${report} +cd ${temp_dir} +cp -i \ + ${other_dir}/${other_project}.SQ${other_version} \ + ${other_dir}/${other_project}.AR${other_version} \ + ${other_dir}/${other_project}.RL${other_version} \ + ${other_dir}/${other_project}.TG${other_version} \ + ${other_dir}/${other_project}.CC${other_version} \ + . +if ( $status ) then + echo stealdata: failed to copy other database | tee -a ${report} + goto abort +endif + + +# +# file of file names in other database +# +set other_files = ${other_project}.all +set other_useless = ${other_project}.useless +set other_useful = ${other_project}.useful +set other_new = ${other_project}.fn.$$ + +# +# extract gel readings +# +set option_extract = 32 +# +# screen against vector +# +set option_screen = 18 +# +echo Extracting gel readings from ${other_project} and | tee -a ${report} +echo " screening against consensus from ${project}" | tee -a ${report} +${other_db_type} << EOF > /dev/null + +y +${other_project} +${other_version} + +${option_extract} +${other_files} +n + +${option_screen} +y +${other_files} +${other_useless} +${consensus} +30 + +! +EOF +if ( $status ) then + echo stealdata: failed to extact or screen readings | tee -a ${report} + goto abort +endif + +# +# determine useful files in other project +# +echo Determining useful gel readings | tee -a ${report} +sort ${other_files} | tr -d ' ' > ${other_files}.nice +sort ${other_useless} | tr -d ' ' > ${other_useless}.nice +comm -23 ${other_files}.nice ${other_useless}.nice > ${other_useful} + +# +# create report header +# +echo "" | tee -a ${report} +echo Useful gel readings: | tee -a ${report} + +# +# determine new files +# +touch ${other_new} +set useful = 0 +foreach x ( `cat ${other_useful}` ) + if ( -e ../$x ) then + echo $x | tee -a ${report} + else + echo $x "- NEW" | tee -a ${report} + echo $x >> ${other_new} + @ useful ++ + endif +end + +if ( $useful == 0 ) then + echo No new gel readings | tee -a ${report} + goto done +endif + +# +# transfer sequences +# +if (${operation} == "s") then + echo "" | tee -a ${report} + echo "Transfering sequence files: " | tee -a ${report} + foreach x ( `cat ${other_new}` ) + cp -i ${other_dir}/${x} .. + echo ${other_dir}/${x} | tee -a ${report} + end + # file of file names + mv ${other_new} .. + echo "" | tee -a ${report} + echo File of file names is ${other_new} | tee -a ${report} +endif + +# +# transfer sequences and traces +# +if (${operation} == "a") then + echo "" | tee -a ${report} + echo "Transfering sequence and trace files: " | tee -a ${report} + foreach x ( `cat ${other_new}` ) + # copy sequence file + cp -i ${other_dir}/${x} .. + echo ${other_dir}/${x} | tee -a ${report} + foreach file ( ${other_dir}/${x}{${trace_suffixes}} ) + if (-e $file) then + cp -i $file .. + echo $file | tee -a ${report} + endif + end + end + # file of file names + mv ${other_new} .. + echo "" | tee -a ${report} + echo File of file names is ${other_new} | tee -a ${report} +endif + +# +# transfer sequences and link traces +# +if (${operation} == "l") then + echo "" | tee -a ${report} + echo "Transfering sequence and trace files: " | tee -a ${report} + set files = `cat ${other_new}` + pushd .. >& /dev/null + foreach x ( $files ) + # copy sequence file + cp -i ${other_dir}/${x} . + echo ${other_dir}/${x} | tee -a ${report} + foreach file ( ${other_dir}/${x}{${trace_suffixes}} ) + # link to traces + if (-e $file) then + ln -s $file + echo $file | tee -a ${report} + endif + end + end + popd >& /dev/null + # file of file names + mv ${other_new} .. + echo "" | tee -a ${report} + echo File of file names is ${other_new} | tee -a ${report} +endif + +done: +# +# cleanup +# +echo Cleaning up | tee -a ${report} +cd .. +/bin/rm -r ${temp_dir} + +# +# Finish up +# +echo "" | tee -a ${report} +echo "Completed" | tee -a ${report} + +# +# mail report +# +echo Mailing you a report +mail `whoami` < ${report} + + +exit 0 + + +# +# +# +abort: + echo stealdata aborted `date` | tee -a ${report} + if ( $?home_dir ) then + cd $home_dir + echo Cleaning up | tee -a ${report} + /bin/rm -r ${temp_dir} + endif + + exit 1 diff --git a/src/scripts/stealdata.stl b/src/scripts/stealdata.stl new file mode 100644 index 0000000..399d1ae --- /dev/null +++ b/src/scripts/stealdata.stl @@ -0,0 +1,441 @@ +#! /bin/csh -f +# +# stealdata (St.Louis Version) +# +# Borrow data and files from a neighbouring cosmid database +# +# St.Louis version - +# 1. prompts users for projects, versions, etc +# 2. has to handle different reading/trace name conventions: +# +# Site Reading Trace +# ------------ ---------- -------------------- +# Cambridge,UK .{SCF,ABI,ALF> +# St.Louis .seq +# +# +# +# Change log: +# +# v1.2 Handle dap and bap databases +# v1.3 Provide linking option +# v1.4 Under no circumstances link sequence files +# v1.5 Bug in option "l". Now works when databases have same name +# v1.6 To work with new version of bap +# v1.7 Variable report now set correctly +# v1.7.s St.Louis Version +# v1.8 bug in fudge +# + +onintr abort + +# +# set defaults here +# +# NOTE: "other" refers to the project you want to pinch data off +# NOTE: db_type and other_db_type must be either "dap" or "bap" +# + +echo -n "project name? (e.g. ZK1236)" +set project = $< +echo -n "project version (e.g. 0) " +set version = $< +echo -n "database type? (dap or bap) " +set db_type = $< +echo -n "other project directory? (e.g. ~rick/ZK353 or ~rick/ZK112/assembly) " +set other_dir = $< +echo -n "other project name? (e.g. ZK353) " +set other_project = $< +echo -n "other project version? (e.g. 0) " +set other_version = $< +echo -n "other database type? (dap or bap) " +set other_db_type = $< + +#set project = F54G8 +#set version = 0 +#set db_type = dap +#set other_dir = ~kt/ZC84 +#set other_project = ZC84 +#set other_version = 0 +#set other_db_type = bap + +# +# available operations are: +# r = report useful readings (response mailed) +# s = "r" + copy useful sequences only +# a = "r" + copy useful sequences AND traces +# l = "r" + copy useful sequences and LINK traces +# default = "s" +# +# set operation = "s" + +echo "Choose desired operation: " +echo " Available operations are:" +echo " r = report useful readings (response mailed)" +echo " s = r + copy useful sequences only" +echo " a = r + copy useful sequences AND traces" +echo " l = r + copy useful sequences and LINK traces" +set operation = $< + + + + +# +# other - DON'T TOUCH ... only used for England +# +set trace_suffixes = ALF,RES,SCF + +# +# check defaults +# +if (! -e ${project}.SQ${version}) then + echo Project ${project} version ${version} does not exist + exit 1 +endif + +if (! -d ${other_dir}) then + echo Directory ${other_dir} does not exist + exit 1 +endif + +if (! -e ${other_dir}/${other_project}.SQ${other_version}) then + echo Project ${other_dir}/${other_project} version ${other_version} does not exist + exit 1 +endif + +# +# Report file +# + +set report = $cwd/_steal.rep.${project} +set count = 0 +while ( -e $report ) + # echo Report file ${report} already exists... + @ count ++ + set report = $cwd/_steal.rep.${project}.${count} +end + +touch ${report} + +# +# Output details of stealdata run +# +cat << EOF | tee -a ${report} +Stealdata v1.8 +Borrow data from neighbouring cosmid database + +project = ${project} +version = ${version} +db_type = ${db_type} +other_dir = ${other_dir} +other_project = ${other_project} +other_version = ${other_version} +other_db_type = ${other_db_type} +operation = ${operation} +EOF + +switch ( ${operation} ) +case "r": + echo " ( report only )" | tee -a ${report} + breaksw +case "s": + echo " ( copy sequences only, report )" | tee -a ${report} + breaksw +case "a": + echo " ( copy sequences and traces, report )" | tee -a ${report} + breaksw +case "l": + echo " ( copy sequences and link traces, report )" | tee -a ${report} + breaksw +default: + echo " ( unknown - report only )" | tee -a ${report} + set operation = "r" + breaksw +endsw +echo "" | tee -a ${report} + +# +# create a temporary directory +# +echo Creating a temporary directory | tee -a ${report} +set temp_dir = _steal.dir.${other_project} +set count = 0 +while (-e ${temp_dir}) + echo Temporary directory ${temp_dir} already exists | tee -a ${report} + @ count ++ + set temp_dir = _steal.dir.${other_project}.${count} +end + +set home_dir = `pwd` +mkdir ${temp_dir} + +# +#consensus for database +# +set consensus = _steal.seq.${project} +set count = 0 +while ( -e $consensus ) + echo Consensus file ${consensus} already exists | tee -a ${report} + @ count ++ + set consensus = _steal.seq.${project}.${count} +end + +# +# determine consensus +# +echo Determining the consensus for project ${project} version ${version} | tee -a ${report} +# +# FUDGE!!! +# The third question in option 8 (Calculate consensus) +# has changed in the latest version of bap. +# Was: +# ? Make another consensus (y/n) (y) +# Now is: +# ? Staden format (y/n) (y) = +# +# Set fudge to reflect the appropriate choice +# +if ( ${db_type} == "bap" ) then + set fudge = y +else + set fudge = n +endif +${db_type} << EOF > /dev/null + +y +${project} +${version} + +8 +${consensus} +y +${fudge} + +! +EOF +if ( $status ) then + echo stealdata: failed to make consensus | tee -a ${report} + goto abort +endif + + +# +# move consensus to temp_dir +# +mv ${consensus} ${temp_dir} +if ( $status ) then + echo stealdata: failed to move consensus to temp_dir | tee -a ${report} + goto abort +endif + + +# +# copy other database to this directory +# +echo Copying project ${other_project} version ${other_version} | tee -a ${report} +cd ${temp_dir} +cp -i \ + ${other_dir}/${other_project}.SQ${other_version} \ + ${other_dir}/${other_project}.AR${other_version} \ + ${other_dir}/${other_project}.RL${other_version} \ + ${other_dir}/${other_project}.TG${other_version} \ + ${other_dir}/${other_project}.CC${other_version} \ + . +if ( $status ) then + echo stealdata: failed to copy other database | tee -a ${report} + goto abort +endif + + +# +# file of file names in other database +# +set other_files = ${other_project}.all +set other_useless = ${other_project}.useless +set other_useful = ${other_project}.useful +set other_new = ${other_project}.fn.$$ + +# +# extract gel readings +# +set option_extract = 32 +# +# screen against vector +# +set option_screen = 18 +# +echo Extracting gel readings from ${other_project} and | tee -a ${report} +echo " screening against consensus from ${project}" | tee -a ${report} +${other_db_type} << EOF > /dev/null + +y +${other_project} +${other_version} + +${option_extract} +${other_files} +n + +${option_screen} +y +${other_files} +${other_useless} +${consensus} +30 + +! +EOF +if ( $status ) then + echo stealdata: failed to extact or screen readings | tee -a ${report} + goto abort +endif + +# +# determine useful files in other project +# +echo Determining useful gel readings | tee -a ${report} +# lfw added awk intermediary +# need to get a .seq on the end of all of the filenames...the output +# from dap/bap would have truncated names +sort ${other_files} | tr -d ' ' | awk 'BEGIN{FS=".";}{ print $1"."$2".seq";}' > ${other_files}.nice +sort ${other_useless} | tr -d ' ' | awk 'BEGIN{FS=".";}{ print $1"."$2".seq";}' > ${other_useless}.nice +comm -23 ${other_files}.nice ${other_useless}.nice > ${other_useful} + +# +# create report header +# +echo "" | tee -a ${report} +echo Useful gel readings: | tee -a ${report} + +# +# determine new files +# +touch ${other_new} +set useful = 0 +foreach x ( `cat ${other_useful}` ) + if ( -e ../$x ) then + echo $x | tee -a ${report} + else + echo $x "- NEW" | tee -a ${report} + echo $x >> ${other_new} + @ useful ++ + endif +end + +if ( $useful == 0 ) then + echo No new gel readings | tee -a ${report} + goto done +endif + +# +# transfer sequences +# +if (${operation} == "s") then + echo "" | tee -a ${report} + echo "Transfering sequence files: " | tee -a ${report} + foreach x ( `cat ${other_new}` ) + cp -i ${other_dir}/${x} .. + echo ${other_dir}/${x} | tee -a ${report} + end + # file of file names + mv ${other_new} .. + echo "" | tee -a ${report} + echo File of file names is ${other_new} | tee -a ${report} +endif + +# +# transfer sequences and traces +# +if (${operation} == "a") then + echo "" | tee -a ${report} + echo "Transfering sequence and trace files: " | tee -a ${report} + +# foreach x ( `cat ${other_new} | sed 's/.seq//g' ` ) +# change by lfw for our sequence file naming convention + foreach x ( `cat ${other_new} | sed 's/.seq//g' ` ) + # copy sequence file + cp -i ${other_dir}/${x} .. + echo ${other_dir}/${x} | tee -a ${report} +# foreach file ( ${other_dir}/${x}{${trace_suffixes}} ) +# change by lfw for our sequence file naming convention + foreach file ( ${other_dir}/${x} ) + if (-e $file) then + cp -i $file .. + echo $file | tee -a ${report} + endif + end + end + # file of file names + mv ${other_new} .. + echo "" | tee -a ${report} + echo File of file names is ${other_new} | tee -a ${report} +endif + +# +# transfer sequences and link traces +# +if (${operation} == "l") then + echo "" | tee -a ${report} + echo "Transfering sequence and trace files: " | tee -a ${report} +#change by lfw so that it accomodates our sequence file names + set files = `cat ${other_new} | sed 's/.seq//g' ` + pushd .. >& /dev/null + foreach x ( $files ) + # copy sequence file + cp -i ${other_dir}/${x} . + echo ${other_dir}/${x} | tee -a ${report} +# foreach file ( ${other_dir}/${x}{${trace_suffixes}} ) +# change by lfw so that it accomodates our sequence and trace file names + foreach file ( ${other_dir}/${x} ) + # link to traces + if (-e $file) then + ln -s $file + echo $file | tee -a ${report} + endif + end + end + popd >& /dev/null + # file of file names + mv ${other_new} .. + echo "" | tee -a ${report} + echo File of file names is ${other_new} | tee -a ${report} +endif + +done: +# +# cleanup +# +echo Cleaning up | tee -a ${report} +cd .. +/bin/rm -r ${temp_dir} + +# +# Finish up +# +echo "" | tee -a ${report} +echo "Completed" | tee -a ${report} + +# +# mail report +# +echo Mailing you a report +mail `whoami` < ${report} + + +exit 0 + + +# +# +# +abort: + echo stealdata aborted `date` | tee -a ${report} + if ( $?home_dir ) then + cd $home_dir + echo Cleaning up | tee -a ${report} + /bin/rm -r ${temp_dir} + endif + + exit 1 + + diff --git a/src/squirrel-1.4/DISCLAIMER b/src/squirrel-1.4/DISCLAIMER new file mode 100644 index 0000000..5f4b1e8 --- /dev/null +++ b/src/squirrel-1.4/DISCLAIMER @@ -0,0 +1,17 @@ +DISCLAIMER + +Squirrel was written by Simon Dear (MRC Laboratory of Molecular +Biology) as a tool to automate the processing of data on the C.elegans +sequencing project. It has been used extensively since May 1992. + +The software is not generally applicable to other sequencing projects +without extensive modification. We would like to encourage other +groups to build similar systems based on our version of squirrel. You +are free to use and modify the squirrel software as you see fit. We do +not accept any responsibility for the software as it is distributed or +after it has been modified by you. + + + + + sd@mrc-lmb.cam.ac.uk diff --git a/src/squirrel-1.4/bin/README b/src/squirrel-1.4/bin/README new file mode 100644 index 0000000..13dc411 --- /dev/null +++ b/src/squirrel-1.4/bin/README @@ -0,0 +1,29 @@ +dir: $SQUIRREL/bin/ +date: Thu May 06 14:18:51 BST 1993 + + +The following programs must be installed in this directory: + +expGetSeq + For extracting the sequence and trace clipping information from + trace files. + Sources in $STADENROOT/src/expGetSeq/ + + +vepe + For excising vector from a sequence in experiment file format. + Sources in $STADENROOT/src/vepe/ + + +getABISampleName + For retrieving the ABI Sample Name from an ABI trace file. + Sources in $STADENROOT/src/abi/ + + +makeSCF + For converting a trace file to SCF format. + Sources in $STADENROOT/src/scf/ + +update_subclones + For updating the $SQUIRREL/tables/subclones database + Sources in $STADENROOT/src/update_subclones/ diff --git a/src/squirrel-1.4/doc/DISCLAIMER b/src/squirrel-1.4/doc/DISCLAIMER new file mode 100644 index 0000000..5f4b1e8 --- /dev/null +++ b/src/squirrel-1.4/doc/DISCLAIMER @@ -0,0 +1,17 @@ +DISCLAIMER + +Squirrel was written by Simon Dear (MRC Laboratory of Molecular +Biology) as a tool to automate the processing of data on the C.elegans +sequencing project. It has been used extensively since May 1992. + +The software is not generally applicable to other sequencing projects +without extensive modification. We would like to encourage other +groups to build similar systems based on our version of squirrel. You +are free to use and modify the squirrel software as you see fit. We do +not accept any responsibility for the software as it is distributed or +after it has been modified by you. + + + + + sd@mrc-lmb.cam.ac.uk diff --git a/src/squirrel-1.4/doc/ExperimentFile.format b/src/squirrel-1.4/doc/ExperimentFile.format new file mode 100644 index 0000000..183a7e7 --- /dev/null +++ b/src/squirrel-1.4/doc/ExperimentFile.format @@ -0,0 +1,303 @@ +Experiment file format Simon Dear, 15 February 1993 +--------------------------------------------------------------------- + +The experiment file will contain every piece of information that will +ever needed to be known for the initial sequence processing, assembly, +oligo primer selection and automated walking. This does not mean that +all records in the file will be stored in the new style database, as +many are clearly not relevant after the preprocessing stages. + + +Records in file +--------------- + +The order of records in the file is not important. They are listed +here in alphabetical order: + +CC (comment line) +CF (Cloning vector sequence File) +CN (Clone Name) +CS (Cloning vector Sequence present in sequence) +CV (Cloning Vector type) +DR (Direction of Read) +DT (DaTe of experiment) +EN (Experiment Name) +EX (EXperimental notes) +FM (sequencing vector Fragmentation Method) +ID (IDentifier) +LN (Local format trace file Name) +LT (Local format trace file Type) +MC (MaChine on which experiment ran) +MN (Machine generated trace file Name) +MT (Machine generated trace file Type) +OP (OPerator) +PN (Primer Name) +PS (Processing Status) +QL (poor Quality sequence present at Left (5') end) +QR (poor Quality sequence present at Right (3') end) +SC (Sequencing vector Cloning site) +SF (Sequencing vector sequence File) +SI (Sequencing vector Insertion length) +SL (Sequencing vector sequence present at Left (5') end) +SP (Sequencing vector Primer site (relative to cloning site)) +SQ (SeQuence) +SR (Sequencing vector sequence present at Right (3') end) +SS (Screening Sequencing) +ST (STrands) +SV (Sequencing Vector type) +TN (Template Name) + + +Explanation of records +---------------------- + +Record: CC (comment line) +Format: CC string +Explanation: +A general comment line +be used by vep. + +Record: CF (Cloning vector sequence File) +Format: CF string +Explanation: +The name of the file containing the sequence of the cloning vector, to +be used by vep. + +Record: CN (Clone Name) +Format: CN string +Explanation: +The name of the physical map clone. The new database will allow +assembly of sequences from overlapping clones. Knowing the clone name +provides gap with mapping information about the sequence. + +Record: CS (Cloning vector Sequence present in sequence) +Format: CS range +Explanation: +Regions of sequence considered to be cloning vector. Used in assembly +to exclude unwanted sequence. + +Record: CV (Cloning Vector type) +Format: CV string +Explanation: +The type of the cloning vector used. Required for vep + +Record: DR (Direction of Read) +Format: DR direction +Explanation: +Which of forward or reverse primers were used. Useful for oligo primer +selection, and automated walking. Allows mapping of forward and +reverse reads off same template. + +Record: DT (DaTe of experiment) +Format: DT dd-mon-yyyy +Explanation: +The date on which the experiment file was created. Not used anywhere. + +Record: EN (Experiment Name) +Format: EN string +Explanation: +The name given to the experiment. Allows disassociation of the name of +the experiment file and the name that gets used by gap. + +Record: EX (EXperimental notes) +Format: EX string +Explanation: +Free format comment for additional information. Not used anywhere. + +Record: FM (sequencing vector Fragmentation Method) +Format: FM string +Explanation: +Fragmentation method used to create subclone (sequencing vector) +library. Non used anywhere. + +Record: ID (IDentifier) +Format: ID string +Explanation: +Same as EN (Experiment Name). This field is to make the experiment +file look like it is in EMBL format. + +Record: LN (Local format trace file Name) +Format: LN string +Explanation: +The name of the local format trace file. This information is passed +onto gap, and allows for local formats to be used. + +Record: LT (Local format trace file Type) +Format: LT string +Explanation: +The type of the local trace file type. + +Record: MC (MaChine on which experiment ran) +Format: MC string +Explanation: +The lab's name for the sequencing machine used to create the data. Is +this useful? + +Record: MN (Machine generated trace file Name) +Format: MN string +Explanation: +The name of the trace file generated by the sequencing machine MC. + +Record: MT (Machine generated trace file Type) +Format: MT string +Explanation: +The type of MN. + +Record: OP (OPerator) +Format: OP string +Explanation: +Someone's name, possibly the person who ran the script. Not used anywhere. + +Record: PN (Primer Name) +Format: PN string +Explanation: +Name of primer used, using local naming convention. Could be a +universal primer. May be useful one day. Not used currently. + +Record: PS (Processing Status) +Format: PS explanation +Explanation: +Indication of processing status. The presence of this entry indicates +failure of some kind, and the experiment file should not be further +processed. + +Record: QL (poor Quality sequence present at Left (5') end) +Format: QL position +Explanation: +The sequence up to and including the base at the marked position are +considered too poor quality to be considered. May overlap with other +marked sequences - CS, SL or SR. Used in assembly to exclude unwanted +sequence. + +Record: QR (poor Quality sequence present at Right (3') end) +Format: QR position +Explanation: +The sequence from and including the base at the marked position to the +end are considered too poor quality to be considered. May overlap with +other marked sequences - CS, SL or SR. Used in assembly to exclude +unwanted sequence. + +Record: SC (Sequencing vector Cloning site) +Format: SC position +Explanation: +The cloning site of the sequence vector, based on local knowledge of +the sequence vector. Used by vep. + +Record: SF (Sequencing vector sequence File) +Format: SF string +Explanation: +The name of the file containing the sequence of the sequencing vector, +to be used by vep. + +Record: SI (Sequencing vector Insertion length) +Format: SI range +Explanation: +Suspected insertion length of subclone. Useful for selecting templates +in oligo primer selection. + +Record: SL (Sequencing vector sequence present at Left (5') end) +Format: SL position +Explanation: +The sequence up to and including the base at the marked position are +considered to be sequencing vector. Used in assembly to exclude +unwanted sequence. + +Record: SP (Sequencing vector Primer site (relative to cloning site)) +Format: SP position +Explanation: +Location of the primer using to sequence relative to cloning site. +Used by vep. + +Record: SQ (SeQuence) +Format: SQ \nsequence...\n\\\n +Explanation: +Complete sequence, as determined by the sequencing machine. + +Record: SR (Sequencing vector sequence present at Right (3') end) +Format: SR position +Explanation: +The sequence from and including the base at the marked position to the +end are considered to be sequencing vector. Used in assembly to +exclude unwanted sequence. + +Record: SS (Screening Sequencing) +Format: SS string +Explanation: +Sequences to screen sequence against. To screen against more that one +sequence, it is permissible to include more that one SS line. The +field is used by the script screen-against-vector from within squirrel. +Experiments partially matching a screen file will have a PS line added +to the end. + +Record: ST (STrands) +Format: ST number +Explanation: +Indicating whether this is a single or double stranded template. This +is useful in oligo primer selection, for deducing suitable templates. + +Record: SV (Sequencing Vector type) +Format: SV string +Explanation: +Name of sequencing vector used in experiment. Allows preference lists +to be set up for choosing templates in selecting oligos. + +Record: TN (Template Name) +Format: TN string +Explanation: +The name of the template used in the experiment. + + + +Example File +------------ + +The information so far included here is easily derivable from the +trace file, using various scripts and a few look-up tables. This +example is based on one created automatically: + + +ID h4a01h6.s1 +EN h4a01h6.s1 +TN h4a01h6 +EX lane 18, run time 10 hrs +MN Sample 18 +MC A +MT ABI +LN h4a01h6.s1SCF +LT SCF +DT 08-Jan-1993 +CC Dir: /home/sd/Mac-files-sd/Results F 16_12_92.2 +OP sd +TN h4a01h6 +SV M13mp18 +SF /home/sd/squirrel/seqs/m13mp18.vec +SI 1000..2000 +SC 6249 +FM shear +PN -21 +DR + +SP 41 +ST 1 +CN 3G9 +CV sCos-1 +CF /home/sd/squirrel/seqs/sCos-1.seq +SS /home/sd/squirrel/seqs/Alu.seqs +SS /home/sd/squirrel/seqs/schrrIII.seq +SQ + GCTTGCATGC CTGCAGGTCG ACTCTAGAGG ATCCCCAACC AGTAAGGCAA CCCCGCCAGC + CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTCAGATC CAGACATGAT + AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGT-AAAAA AATGCTTTAT + TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT + TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT + TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTCT AGTCAAGGCA + CTATACATCA AATATT-CCT TATTAACCCC CTTTACAAAT TTAAAAGGCT -AAAGGGTCC + ACAATTTTTG -GCCTAGGTA TTAATAGCCG GCACTTCTT- TGCCTGTTTT GG-GTAGGG- + AAAACCGGTA TGTTT-TGGT T-TTC +// +CC Quality clipping by newted May-1992 +QL 0 +QR 281 +SL 36 +SR 506 +CS 37..280 +PS Completely cloning vector diff --git a/src/squirrel-1.4/doc/NOTES b/src/squirrel-1.4/doc/NOTES new file mode 100644 index 0000000..e88b20c --- /dev/null +++ b/src/squirrel-1.4/doc/NOTES @@ -0,0 +1,14 @@ +NOTES (C.elegans project) + +1. Sequence files, cloning sites and vepe + +When vepe excises cloning vector, it does not make allowances for the +vector being circular. It also makes no assumptions about the locations +of the splice sites. It is important when setting up the sequence files +($SQUIRREL/seqs) to make allowances for these factors - DO NOT LET THE +SPICE SITE BE TOO NEAR EITHER END OF THE FILE. You may need to edit the +sequence file so that the site lies near the centre of the sequence. + +2. Cosmids and cloning sites +Lorist2 BamHI/G'GATCC// + diff --git a/src/squirrel-1.4/doc/Porting.Overview b/src/squirrel-1.4/doc/Porting.Overview new file mode 100644 index 0000000..cc73874 --- /dev/null +++ b/src/squirrel-1.4/doc/Porting.Overview @@ -0,0 +1,154 @@ +SQUIRREL Overview (Version 1.4) 10 July 1992 Simon Dear +================================================================ + +0. Introduction + +This is intended as a brief overview for those wanting to set up +squirrel on their system. It gives an overview of what is supplied +in the package, what changes must be made, and places where it is +foreseen problems may occur. + + +1. Overview of the squirrel directory structure + +./squirrel + The root of the squirrel tree. To use squirrel V1.4 ensure +that the environment variable SQUIRREL is set to be the path name of +the top of the tree. eg + setenv SQUIRREL /home/BioSW/squirrel + +./squirrel/bin + Contains all of the programs required by squirrel -except- the +assembly program(s) dap(,bap,gap). + +./squirrel/doc + All the existing documentation on squirrel. Of interest are: + SQUIRREL - overview of processing + SysManagement - how to manage squirrel's tables + Read them!!! + +./squirrel/scripts + All the scripts used by squirrel are here. The important one +is `squirrel'. A copy of this should be placed in the user's project +directory and edited so that the environment variables PROJECT and +EDITOR reflect the project name and the user's choice of editor. + +./squirrel/seqs + All the vector sequence files that squirrel V1.4 knows about. + +./squirrel/tables + Information about the sequencing project. See the document +SysManagement and SQUIRREL for details. + + + + + +2. Changes required + +The following changes should be made immediately. They are the obvious +things. There are undoubtedly more subtle changes to be made in the +scripts. + +./squirrel/tables/vector-seqfiles + You may need to add entries to this file. V1.4 assumes that +the files exist in the directory $SQUIRREL/seqs + +./squirrel/tables/databases + Register your users now! + +./squirrel/tables/seqclone-sites + You may need to add entries to this file. WARNING - it is +exceedingly difficult to get the values of the cloning site and +primer positions correct. Ensure that the associated vector file +is in the appropriate sense. + Support for multiple cutters: You will need to do provide +entries for each vector-cutter pair. Eg M13mp18-SmaI. Default values +(eg M13mp18) should be included AFTER more specific ones. Subclones +should include the vector-cutter pair as the sequencing vector. + Multiple forward or reverse primers aren't yet supported. + +./squirrel/tables/subclones + The painful bit. This information will need to be updated on a +regular basis. + +./squirrel/tables/clone_types.awk + Information mapping clones to their cloning vector. + + + + + +3. Assumptions made + +To foresee where squirrel may go wrong, it will pay be be aware of the +assumptions made by the script. + +a) Machines supported + Currently squirrel assumes that you have an ABI machine. The +script has been written so that all ABI specific parts are separated. +If other machines are to be supported, then similarly functioning +scripts to ABI-sess-files.csh (for determining samples and experiments to +be processed) and ABI-exp-files.csh (creation of experiment files - +machine specific bits) will need to be written. + +b) Location of Results folders + Results folders are assumed to be in the directory called: + $HOME/Mac-files-$USER/"Results $MACHINE $DATE" +where $HOME is the user's home directory, $USER is the user's is, +$MACHINE and $DATE are the command line arguments passed to squirrel. +This is evaluated once, in the script squirrel. + +c) Samples to be processed + V1.4 will assume that all files in the results folder matched by the +glob "Sample [0-9][0-9]{,.[0-9]}" are to be processed. + +d) Experiment naming conventions + squirrel makes a few assumptions about the naming convention +of experiments (samples): + ::= . +where: + ::= [a-z]{1,2}[0-9][0-9] + ::= + ::= [fF] {forward read, double stranded} + | [rR] {reverse read, double stranded} + | [^rR] {forward read, single stranded} + ::= {any else} +Because of the way squirrel uses lookup tables to gather its +information, it is essential that the prefix determines +a unique microtitre dish. (ie can't have mtds with names "a10" and +"a1"). I think our naming convention guarentees this. Also, being able +to determine the direction of the read is essential for vepe to work. +The strandedness is determined but isn't currently used. All this is +encoded in the script `embellish-exp-files.csh'. + +e) Only used for shotgun stage + squirrel has only been tested for the shotgun stage. V1.4 is +not guarenteed to work for the walking phase. + +f) Owner of directory + squirrel doesn't check that the user is the ower of the +results folder or the project directory. + +g) Unwanted files + Squirrel assumes that you don't want to keep any of the files +(ABI traces or .Seq files) in the Results folder. Traces are converted +to SCF format by ABI-exp-files.csh. + +h) Software assumptions + You mush have awk and assembly program (dap,bap) to run +squirrel. All other programs are provided. squirrel assumes that you +have a version of the assembly program that writes out reason codes +with the assembly failures, but it doesn't complain if you don't. + +i) That you want to run the entirity of squirrel and not just bits of it + Most of the scripts in squirrel could be run as stand-alone +programs but they weren't written with that intention. With squirrel +you should assume you get all or nothing. + + + + + + + diff --git a/src/squirrel-1.4/doc/README b/src/squirrel-1.4/doc/README new file mode 100644 index 0000000..7a8c300 --- /dev/null +++ b/src/squirrel-1.4/doc/README @@ -0,0 +1,44 @@ + Dir: $SQUIRREL/doc/ +Date: Fri May 07 11:16:27 BST 1993 + +Here is the documentation on squirrel. + + +DISCLAIMER - + Please read first! + +SQUIRREL - An overview of squirrel + A quick guide to the processing phases involved in squirrel. + Example session files, experiment files, reports and squirrel + databases (tables) are given. + +SysManagement - Maintaining the squirrel databases + An in depth guide into how to maintain the information squirrel + needs to process automatically. + +Porting.Overview - Getting squirrel working on your system + A brief overview for those wanting to set up squirrel on their + system. It gives an overview of what is supplied in the + package, what changes must be made, and places where it is + foreseen problems may occur. + +ExperimentFile.format - A guide to experiment files + Experiment files are the mechanism information is transferred + between programs in squirrel. Future versions of the Staden + assembly program will read in experiment files directly. This + document is useful for those wanting to produce compatible + files but who do not wish to use squirrel. + +NOTES - General comments + Not much here, though an important note for those adding new + cloning vector files to their squirrel databases. + + +----- + +Further information should be sought from the author. + + + +Simon Dear +sd@mrc-lmb.cam.ac.uk diff --git a/src/squirrel-1.4/doc/SQUIRREL b/src/squirrel-1.4/doc/SQUIRREL new file mode 100644 index 0000000..2e9f37c --- /dev/null +++ b/src/squirrel-1.4/doc/SQUIRREL @@ -0,0 +1,281 @@ +SQUIRREL +---------------------------------------------------------------------- + + squirrel n. small bushy-tailed tree-living rodent; (fig) one + who hoards objects of small value + + + +Squirrel performs all operations on samples in a results folder: +quality clipping, vector removal and assembly. + +Usage: + squirrel machine_letter date + + + +Phase 0: Start up + +Determines the pass number. Squirrel allows several passes on the same +Results folder. + + +Phase 1: Determining samples to be processed (ABI) + +Creates a session file which maps each available sample to its +experiment name (sample name). An example of the session file appears +at the end. + + +Phase 2: Edit the session file + +The user can edit the session file, to correct experiment names, and +to selectively omit entries from further processing + +FROM HERE ON, the process is automatic and requires no user +intervention. + +Phase 2a: Check the edited experiment file for consistency + + +Phase 3: Create experiment files for each entry in the session file. (ABI) + +Only sequencing machine (ABI) specific fields are added to the new file. +In addition, the trace file is converted to our local (SCF) format. + + +Phase 4: Embellish the experiment files. + +Non machine specific attributions. Starting from the experiment name, +it is possible to determine all information about a specific +experiment file. A series of lookup tables is used (see end). + + experiment-name -> (cosmid,insert-size,frag-method,seq-vector) + eg c72a11.s1 -> (C40H1,1000..2000,shear,M13mp18) + + experiment-name -> direction-of-read + eg c72a11.s1 -> forwards + + cosmid -> cloning-vector + eg C40H1 -> pJB8 + + seq-vector -> (clone-site,forward-primer-site,reverse-primer-site) + eg M13mp18 -> (6249,41,-24) + + vector -> vector-file + eg pJB8 -> /nfs/westmorland/home/BioSW/staden/userdata/pjb8.vep + eg M13mp18 -> /nfs/westmorland/home/BioSW/staden/userdata/m13mp18.vec + +The sequence is extracted from the trace (SCF) file, and clipped. +Clipping is now done by examining the traces, using LaDeana's quality +measures. As a fallback mechanism, the old method based on base content +is still used, but only when it gives more conservative values. + +[Old method is: + +To the right of base number 200 and to the left of base 100 everything +is considered good quality upto where there are 2 uncertainties in a +5 base window. The right hand end is ALWAYS knocked back 50 bases, and +never is set greater than base number 450. +] + + +Phase 5: Quality checking. + +Each experiment file is checked for quality. Sequences that, after +quality clipping, are shorter that 100 bases or have greater than 4% +uncertainties are failed. Traces that fail LaDeana's overall quality +check will fail here as the sequence will have zero length. + + +Phase 6: Vector removal + +There are two passes. First to remove sequencing vector, and second to +remove cloning vector. The program "vepe" uses information about vector +and vector sequence files embedded in the experiment file. + + +Phase 7: Transfer files to project directory + +Trace files are directly copied. Experiment files are converted to the +current sequence file format used by the assembly program. If they +have been failed for any reason (completely vector, poor quality) they +are placed in the subdirectoy "Failures". Also, the experiment files are +retained and stored in the subdirectory "Expfiles". Experiments that +already exist are abandoned. + + +Phase 8: Assemble the sequences + +THe current database is temporarily copied to a working version ('X'), into +which the sequences are assembled. If the assembly completes without +error, the current version is updated. + + +Phase 10: Reporting + +A report is produced detailing the history of each experiment file. +Copies the report are left in subdirectory "Logs" of the project +directory. +Each experiment is given a processing status, which is one of: + + OK - the experiment ended up in the database + Rejected - tried to assembly the experiment but failed + Failed - the experiment was not considered suitable for assembly + (too short, too poor quality, completely vector, etc) +Abandoned - not processed this time because something went wrong + (missing entries in tables, experiment already exists, etc) +Discarded - no information was obtainably for this experiment + (no sequence or trace data) + + + +Phase 9: Tidying up + +All samples that were not abandoned are removed from the transfer +directory. + + + +Example session file +-------------------- + +# SQUIRREL Version 1.1 +# +# Processing /home/sd/Mac-files-sd/Results A 4_10_92.2 +# Project TESTDB +# Started at Wed Apr 15 13:04:58 BST 1992 +# Pass 1 +# +# You may... +# + delete lines you don't wish to be processed +# + change the experiment name +# But DON'T... +# - create additional lines +# - introduce white space +# +Sample 01:c68a1.s1 +Sample 02:c68a2.s1 +Sample 03:c68a3.s1 +Sample 04:c68a4.s1 +Sample 05:c68a5.s1 +Sample 06:c68a6.s1 +Sample 07:c68a7.s1 +Sample 08:c68a8.s1 +Sample 09:c68a9.s1 +Sample 10:c68a10.s1 +Sample 11:c68a11.s1 +Sample 12:c68a12.s1 +Sample 13:c68b1.s1 +Sample 14:c68b2.s1 +Sample 15:c68b3.s1 +Sample 16:c68b4.s1 +Sample 17:c68b5.s1 +Sample 18:c68b6.s1 +Sample 19:c68b7.s1 +Sample 20:c68b8.s1 +Sample 21:c68b9.s1 +Sample 22:c68b10.s1 +Sample 23:c68b11.s1 +Sample 24:c68b12.s1 +Sample 25:c68c1.s1 +Sample 26:c68c2.s1 +Sample 27:c68c3.s1 +Sample 28:c68c4.s1 +Sample 29:c68c5.s1 +Sample 30:c68c6.s1 +Sample 31:c68c7.s1 +Sample 32:c68c8.s1 +Sample 33:c68c9.s1 +Sample 34:c68c10.s1 +Sample 35:c68c11.s1 +Sample 36:c68c12.s1 + + +Example Lookup tables +--------------------- + +experiment-name -> (cosmid,insert-size,frag-method,seq-vector) + +c40 K01F9 1000..2000 shear M13mp18 +c41 K01F9 1000..2000 shear M13mp18 +c42 F31H8 1000..2000 shear M13mp18 (Cosmid+=ZC84) +c43 C18H2 1000..2000 shear M13mp18 +c44 K01B6 1000..2000 shear M13mp18 +c45 ZK512 9000..14000 shear pUC118 +c46 K01B6 1000..2000 shear M13mp18 +c47 M01A8 1000..2000 shear M13mp18 +c48 ZK512 9000..14000 shear pUC118 +c49 ZK507 6000..9000 shear pUC118 +c50 M01A8 1000..2000 shear M13mp18 + + +seq-vector -> (clone-site,forward-primer-site,reverse-primer-site) + +M13mp18 6249 41 -24 +pBS 2305 46 -79 + + +vector -> vector-file + +Lambda /nfs/westmorland/home/BioSW/staden/userdata/lambda.seq +Lorist2 /nfs/westmorland/home/BioSW/staden/userdata/lorist2.vep +Lorist6 /nfs/westmorland/home/BioSW/staden/userdata/lorist6.vep +M13mp18 /nfs/westmorland/home/BioSW/staden/userdata/m13mp18.vec +M13mp7 /nfs/westmorland/home/BioSW/staden/userdata/m13mp7.seq +pBS /nfs/westmorland/home/BioSW/staden/userdata/blue.vep +pJB8 /nfs/westmorland/home/BioSW/staden/userdata/pjb8.vep +pUC18 /nfs/westmorland/home/BioSW/staden/userdata/puc18.vec + + +For simplicity of use, these are converted to awk programs. The +mapping of (cosmid -> cloning-vector) only exists in its awk form, and +is not included here. + + + +Example Report +-------------- + +# SQUIRREL Version 1.1 +# Processing /home/sd/Mac-files-sd/Results A 4_10_92.2 +# Project TESTDB +# Started at Wed Apr 15 18:35:01 BST 1992 +# Pass 4 +# +Sample 01: c68a1.s1 Failed Trace quality +Sample 02: c68a2.s1 OK Assembled +Sample 03: c68a3.s1 Failed Trace quality +Sample 04: c68a4.s1 Failed Trace quality +Sample 05: c68a5.s1 Failed Trace quality +Sample 06: c68a6.s1 Failed Trace quality +Sample 07: c68a7.s1 Failed Completely cloning vector +Sample 08: c68a8.s1 OK Assembled +Sample 09: c68a9.s1 OK Assembled +Sample 10: c68a10.s1 Failed Trace quality +Sample 11: c68a11.s1 OK Assembled +Sample 12: c68a12.s1 OK Assembled +Sample 13: c68b1.s1 OK Assembled +Sample 14: c68b2.s1 OK Assembled +Sample 15: c68b3.s1 Failed Completely cloning vector +Sample 16: c68b4.s1 OK Assembled +Sample 17: c68b5.s1 Failed Trace quality +Sample 18: c68b6.s1 Rejected Did not assemble : failed to assemble and not entered +Sample 19: c68b7.s1 Failed Completely sequencing vector +Sample 20: c68b8.s1 OK Assembled +Sample 21: c68b9.s1 OK Assembled +Sample 22: c68b10.s1 Failed Trace quality +Sample 23: c68b11.s1 OK Assembled +Sample 24: c68b12.s1 OK Assembled +Sample 25: c68c1.s1 Failed Trace quality +Sample 26: c68c2.s1 OK Assembled +Sample 27: c68c3.s1 Failed Completely cloning vector +Sample 28: c68c4.s1 Failed Trace quality +Sample 29: c68c5.s1 OK Assembled +Sample 30: c68c6.s1 Failed Completely cloning vector +Sample 31: c68c7.s1 OK Assembled +Sample 32: c68c8.s1 Failed Completely cloning vector +Sample 33: c68c9.s1 OK Assembled +Sample 34: c68c10.s1 Failed Trace quality +Sample 35: c68c11.s1 Failed Trace quality +Sample 36: c68c12.s1 Failed Completely sequencing vector diff --git a/src/squirrel-1.4/doc/SysManagement b/src/squirrel-1.4/doc/SysManagement new file mode 100644 index 0000000..60ed20f --- /dev/null +++ b/src/squirrel-1.4/doc/SysManagement @@ -0,0 +1,260 @@ + SQUIRREL + System Management + + Simon Dear + 29 June 1992 + + +0. Introduction +--------------- + +Squirrel is a complicated script that gathers together information +about an experiment and then assembles the processed sequences into a +user's database. The philosophy behind squirrel is that there should +be minimal user interaction. In actuality, all that need be entered is +little more than the name of directory containing data to be +processed. + +Ease of use for the user often means extra work for someone else. With +squirrel this is no exception. Squirrel requires the maintenance of +several small files. This document describes these files and the +procedure for keeping them updated. + +Squirrel needs to gather additional information about: + + a) subclone libraries + b) vectors used to clone each cosmid + c) sequencing vector clone sites + d) files containing vector sequence + e) user's databases + +For a sequencing project the data on (b)-(d) will be fairly static and +quite small. Data on (a) will be frequently updated as new libraries +are going to be continually created. Data on (e) will be updated +occasionally; every time new databases are started. + +For C-Shell programming simplicity and uniformity the data is +encapsulated in simple awk programs. + +We sometimes need to look up information which is a generalisation +about a specific example. For example, we may be want data on a +subclone e06b11 where data is held for all subclones starting e06. The +pattern matching abilities of awk are ideal for this. + +We also sometime need to determine information about a specific +example, where it is not possible to make general inferences using +simple rules. An example is data on (b), which reflects the +changing strategies during genome mapping. Building this data into an +awk program is a very simple task. See Appendix 1. + +However, is it not desirable to maintain awk programs. Small typing +mistakes can result in a program with severe errors. For (a), and +(c)-(e) it is more convenient to enter the data into a text file, and +have the awk program automatically created from it using the UNIX make +facility. In the case of (b), there is no escaping having an awk +program. It is therefore fortunate that this data will not +substantially change during the lifetime of the sequencing project. + +The text files for (a)-(e) can be found in the directory + + $SQUIRREL/tables + (/nfs/tode/home2/users/wormpub/BioSW/squirrel/tables) + +During the rest of this document it is assumed that the person +performing the updates is logged in as user wormpub and that they have +changed to this directory. + + + + +1. Updating information on subclone libraries +--------------------------------------------- + +The information on subclone libraries can be updated in two ways. + +First, the file ``subclones'' can be modified using an editor. It is +important that information is entered in exactly the right format. The +fields of this file are: + + ... + + +An example: + e50 F54C8 1000..2000 shear M13mp18 + +Alternatively the program update_subclones can be used. It provides +sensible default values for all the fields and checks that the +data specified is valid. The program can be run by any user from any +directory. It assumes that the environment variable SUBCLONES holds +the full path name of the file ``subclones''. + +Here is an example of an update of the subclone file using +update_subclones. Default values are given in square brackets. +Responding with a question mark ('?') at any time provides information +of what is expected. + + + Maintain subclone database + + Database=/nfs/al/hgmp0/wormpub/BioSW/staden/tables/subclones + + Microtitre dish [e50] ? + Cosmid [F54C8] ? + Method of fragmentation [shear] ? + Vector [M13mp18] ? + Size range [1000..2000] ? + Comment #1 [] ? + + Here are the values you entered: + + Microtitre dish number: e50 + Cosmid: F54C8 + Fragmentation method: shear + Vector: M13mp18 + Size range: 1000..2000 + + Are these values correct [Yes] ? + + +Once the file has been updated it is important to remake the +awk program required by squirrel. To do this, type: + + make all + + + + +2. Updating information on vectors used to clone each cosmid +------------------------------------------------------------ + +Information on which vectors are used for each cosmid can be found in +the file ``clone_types.awk''. This information is be updated using an +editor, though it is unlikely that it will change much through the +course of the sequencing project. The data is only encapsulated in an +awk program; there is no text file. It is important to update this file +correctly and to ensure that no errors are introduced into the awk +program. The program can be tested for errors by typing something +like: + + echo TEST | awk -f clone_types.awk + + + + +3. Updating information on sequencing vector clone sites +-------------------------------------------------------- + +Information on sequencing vector clone sites is held in the file +``seqclone-sites'', and is updated by manually editing the text file. +The format of the data is: + + + +An example: + pBS 2305 46 -79 + +The positions relate to the sequence held in the file specified in the +``vector-seqfiles'' files. The clone-site is defined as the base +position of the 3' end of the cloning site The primer sites are positions +relative to this. + +To make the awk program required by squirrel, type: + make all + +It is important that this information is correct and that the sequence in +the sequence file is in the correct orientation. Otherwise, the vector +will not be successfully removed from sequence processed by squirrel. + + + + + +4. Updating information on files containing vector sequence +----------------------------------------------------------- + +Information of the files containing vector sequence is help in the file +``vector-seqfiles'', and is updated by manually editing the text file. +The format is: + + + +An example: + pBS /nfs/al/pubseq/pubseq/inhouse/sun-nematode/squirrel/seqs/blue.vep + +To make the awk program required by squirrel, type: + make all + +Note that the file name includes the full path name. All working versions +of sequence files required by squirrel are kept in the $SQUIRREL/seqs +directory. + + + + +5. Updating information on user's databases +------------------------------------------- + +User's databases must be registered with squirrel by including an +entry for the project in the file ``databases''. The format is: + + + +An example: + C38C10 C38C10 C38C10 0 bap kt + +The lookup file is remade by typing: + make all + +The directory is relative to the owner's home directory. The database type +is currently one of "dap" or "bap". So the database for kt's cosmid C38C10 +is ~kt/C38C10/C38C10.??0. + + +A1. Vectors used to clone each cosmid +------------------------------------- + +#clone_types.awk +# +# Determine Clone types from Clone names +# +# 3 February 1992, Simon Dear +# +# Assumes that there is one clone name per line. +# Outputs to stdout clone vector. +# +# Data in this program has been taken from "Clone types in the genome map +# of Caenorhabditis elegans" courtesy by John Sulston. This needs +# revision. +# +# Possible usage: +# set clone-type = `echo $clone | awk -f clone-types.awk` +# +/^A/ { print "Lambda"; next; } +/^ZL/ { print "Lambda"; next; } +/^YSL/ { print "Lambda"; next; } +/^[BCDERM]/ { print "pJB8"; next; } +/^ZC/ { print "pJB8"; next; } +/^K/ { print "LoristB"; next; } +/^T/ { print "Lorist2"; next; } +/^W/ { print "Lorist4"; next; } +/^F/ { print "Lorist6"; next; } +/^ZK/ { + num = substr($1,3) + 0.0; + if (num < 15) { print "unknown"; next; } + if (num <= 56) { print "Lorist6"; next; } + if (num <= 130) { print "Lorist2"; next; } + if (num <= 164) { print "Lorist6"; next; } + if (num <= 177) { print "Lorist2"; next; } + if (num <= 192) { print "Lorist6"; next; } + if (num <= 344) { print "Lorist2"; next; } + if (num <= 354) { print "Lorist6"; next; } + if (num <= 514) { print "Lorist2"; next; } + if (num <= 552) { print "Lorist6"; next; } + if (num <= 596) { print "Lorist2"; next; } + if (num <= 616) { print "Lorist?"; next; } + if (num <= 626) { print "Lorist2"; next; } + if (num <= 692) { print "Lorist6"; next; } + if (num <= 756) { print "Lorist2"; next; } + if (num >= 757) { print "Lorist6"; next; } + } +/^Y/ { print "pYAC4"; next; } diff --git a/src/squirrel-1.4/report/README b/src/squirrel-1.4/report/README new file mode 100644 index 0000000..66977c7 --- /dev/null +++ b/src/squirrel-1.4/report/README @@ -0,0 +1,25 @@ + SQUIZZ + A Squirrel Reporting Program + + Simon Dear + 7 May 1993 + + + +0. Introduction + +Squizz analyses squirrel reports and produces a breakdown summary +detailing how many readings were assembled and failed, and for what +reasons. The average reading length of processed data is also +calculated. + + +1. Usage + +Run squizz in your project directory. Just type: + + $SQUIRREL/report/squizz + +The breakdown summary will be produces quite quickly. The report on +average reading lengths will take a little longer. + diff --git a/src/squirrel-1.4/report/a.awk b/src/squirrel-1.4/report/a.awk new file mode 100644 index 0000000..139f0a5 --- /dev/null +++ b/src/squirrel-1.4/report/a.awk @@ -0,0 +1,16 @@ +BEGIN { dir = "unknown"; pass = "unknown" } +/^# Processing/ { + dir = substr($0,14); +} + +/^# Pass/ { + pass = $3; +} + +/^#/ {next;} + +{ + colon = index($0,":"); + sample = substr($0,1,colon - 1); + rest = substr($0,colon+1); + print dir, sample, ":", pass, ":", rest; } diff --git a/src/squirrel-1.4/report/b.awk b/src/squirrel-1.4/report/b.awk new file mode 100644 index 0000000..a9ba749 --- /dev/null +++ b/src/squirrel-1.4/report/b.awk @@ -0,0 +1,7 @@ +BEGIN { FS = ":"; } +{ + if (lastkey != $1 && lastkey != "") print last; + lastkey = $1; + last = $0; +} +END { print last; } diff --git a/src/squirrel-1.4/report/c.awk b/src/squirrel-1.4/report/c.awk new file mode 100644 index 0000000..653ff4e --- /dev/null +++ b/src/squirrel-1.4/report/c.awk @@ -0,0 +1,29 @@ +{ + class = $2; + $1 = "" ; $2 = "" ; + reason = substr($0,3); + count++; + ccount[class]++; + rcount[class reason]++; + reazon[class reason] = reason; + klass[class reason] = class; +} + +END { + for (c in ccount) { + if (c=="") { + print "unknown:" + } else { + print c ":" + } + for ( cr in rcount ) { + if (klass[cr] == c) { + if (reazon[cr] == "") reazon[cr]="unknown"; + printf " %-64s%4d\n",reazon[cr],rcount[cr]; + } + } + printf " %-64s%4d\n","TOTAL", ccount[c]; + print ""; + } + printf "%-64s %4d\n", "TOTAL PROCESSED",count; +} diff --git a/src/squirrel-1.4/report/squizz b/src/squirrel-1.4/report/squizz new file mode 100644 index 0000000..a7de940 --- /dev/null +++ b/src/squirrel-1.4/report/squizz @@ -0,0 +1,47 @@ +#! /bin/csh -f + +onintr abort + +set A = $SQUIRREL/report/a.awk +set B = $SQUIRREL/report/b.awk +set C = $SQUIRREL/report/c.awk + +if ( ! -d Logs ) then + echo "No nut hoards for squirrel report" + exit 1 +endif + +# +# Temp files +# +set S_all = /tmp/S_all.$$ +set S_oks = /tmp/S_oks.$$ +set S_lens = /tmp/S_lens.$$ + +# +# Remove temporary files +# +/bin/rm -f $S_all $S_oks $S_lens + +# Distill information from several passes +awk -f $A Logs/log* | sort | awk -f $B | sed 's/[^:]*:[^:]*: *//' > $S_all + +# Main report +awk -f $C $S_all + +# Determine average lengths +awk '$2=="OK" {print $1;}' $S_all > $S_oks +touch $S_lens +foreach files ( `cat $S_oks` ) + if (-e $files) head -1 $files | awk '{print substr($0,14,6);}' >> $S_lens +end +echo "" +echo "AVERAGE READING LENGTH OF ASSEMBLED READS ---->" `awk '{s += $1;} END {print s/NR,"(" NR ")"; }' $S_lens` + +abort: +# +# Remove temporary files +# +/bin/rm -f $S_all $S_oks $S_lens + +exit 0 diff --git a/src/squirrel-1.4/scripts/ABI-exp-files.csh b/src/squirrel-1.4/scripts/ABI-exp-files.csh new file mode 100644 index 0000000..a6f3726 --- /dev/null +++ b/src/squirrel-1.4/scripts/ABI-exp-files.csh @@ -0,0 +1,107 @@ +#! /bin/csh -f +# +# ABI-exp-files +# Creates an experiment file for each trace file in the current +# directory, and a file of filenames for them. +# +# Usage: +# ABI-exp-files session-file fofn_in fofn_out +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +# Usage +if ( $#argv != 3 ) then + echo "Usage: ABI-exp-files session-file fofn_in fofn_out" | tee -a "$SQUIRREL_LOG" + exit 2 +endif + +set sessfile = $1 +if (! -e $sessfile) then + echo "ABI-exp-files: Session file does not exist" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +if ( ! -e $2 ) then + echo "ABI-exp-files: File of file names does not exist" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +set filenames = $3 +if ( -e $filenames) /bin/rm -f $filenames +touch $filenames + +# Directories +if ( $?SQUIRREL_SUB ) then + set TABLES_DIR = $SQUIRREL/tables-$SQUIRREL_SUB +else + set TABLES_DIR = $SQUIRREL/tables +endif + +# Programs and scripts +set GET_ABI_DATE = $SQUIRREL/scripts/ABI-gel-date.awk +set MAKE_SCF_FILE = $SQUIRREL/bin/makeSCF + +# Process each file +echo "" | tee -a "$SQUIRREL_LOG" +echo "Creating experiment file for:" | tee -a "$SQUIRREL_LOG" +foreach expname ( `cat $2` ) + + # specify file names + set file = `awk 'BEGIN {FS=":";} $2 == "'$expname'" { s = $1; } END { print s;}' ${sessfile}` + set expfile = $expname + set tracefile = ${expfile}SCF + + # log + echo $expname | tee -a "$SQUIRREL_LOG" + + # ensure the experiment doesn't already exist in this directory + if ( -e $expfile ) /bin/rm -f $expfile + + # create experiment file + touch $expfile + + # include obvious information + echo "ID $expname" >> $expfile + echo "EN $expname" >> $expfile + echo "MN $file" >> $expfile + echo "MT ABI" >> $expfile + + # Make standard chromatogram file + $MAKE_SCF_FILE -ABI "$file" -output $tracefile + if ( $status ) then + echo Discarded: ${expfile}: Failed to create SCF trace file | tee -a "$SQUIRREL_LOG" + /bin/rm -f $tracefile + goto skip + endif + echo "LN $tracefile" >> $expfile + echo "LT SCF" >> $expfile + + # get date information + # YUK! next to useless + #set DT = `echo $date | awk -f $GET_ABI_DATE` + #if ( $DT == "" ) set DT = `date +%d-%h-19%y` + set DT = `date +%d-%h-19%y` + echo "DT $DT" >> $expfile + # YUK! next to useless + # echo MC "ABI-$machine" >> $expfile + echo "CC Dir: $cwd" >> $expfile + + # update file of file names + echo $expfile >> $filenames + +# skip to here on error +skip: + +end + +exit 0 + + +abort: +# +# interruption - not successful completion +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/ABI-gel-date.awk b/src/squirrel-1.4/scripts/ABI-gel-date.awk new file mode 100644 index 0000000..d6756f6 --- /dev/null +++ b/src/squirrel-1.4/scripts/ABI-gel-date.awk @@ -0,0 +1,24 @@ +# +# A little program to convert dates of the format MM_DD_YY +# into the format DD-Mon-YEAR +# +# EG 7_9_92 -> 9-Jul-1992 +# +BEGIN {FS="_"; month = "Unknown"; } +$1==1 {month = "January";} +$1==2 {month = "February";} +$1==3 {month = "March";} +$1==4 {month = "April";} +$1==5 {month = "May";} +$1==6 {month = "June";} +$1==7 {month = "July";} +$1==8 {month = "August";} +$1==9 {month = "September";} +$1==10 {month = "October";} +$1==11 {month = "November";} +$1==12 {month = "December";} +{ + if (month != "Unknown" && NF == 3) { + print $2 "-" substr(month,1,3) "-19" substr($3,1,2); + } +} diff --git a/src/squirrel-1.4/scripts/ABI-sess-file.csh b/src/squirrel-1.4/scripts/ABI-sess-file.csh new file mode 100644 index 0000000..b23ac52 --- /dev/null +++ b/src/squirrel-1.4/scripts/ABI-sess-file.csh @@ -0,0 +1,86 @@ +#! /bin/csh -f +# +# ABI-sess-file +# Creates a session file for the files in the current working directory +# +# Usage: +# ABI-sess-file session-file +# +# 22-Jan-93 +# Also picks out files of format "Sample [0-9][0-9]{,.[0-9]}" + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +# Usage +if ( $#argv != 1 ) then + echo "Usage: ABI-sess-file session-file" | tee -a "$SQUIRREL_LOG" + exit 2 +endif + +set filenames = $1 +if (-e $filenames) /bin/rm -f $filenames +touch $filenames + +# Programs and scripts +set GET_ABI_NAME = $SQUIRREL/bin/getABISampleName + +# +# Temporary files +# +set file_list = $1 + +# +# Create session file header +# +if ( -e ${file_list} ) /bin/rm -f ${file_list} +touch ${file_list} +head -7 "$SQUIRREL_LOG" | sed 's/^/# /' >> ${file_list} +cat << EOF >> ${file_list} +# +# You may... +# + delete lines you don't wish to be processed +# + change the experiment name +# But DON'T... +# - create additional lines +# - introduce white space +# +EOF + +# +# Build a list of files in this directory and experiment names +# +echo "" | tee -a "$SQUIRREL_LOG" +echo Determining samples to be processed | tee -a "$SQUIRREL_LOG" +foreach file ( Sample*[0-9][0-9]{,.[0-9]} ) + echo -n $file "- " | tee -a "$SQUIRREL_LOG" + + # tidy up as we go - delete Seq files + /bin/rm -f "$file.Seq" + + # get sample name + set abiname = `$GET_ABI_NAME "$file"` + if ( $status ) then + echo "" | tee -a "$SQUIRREL_LOG" + echo Discarded: : Cannot determine sample name for $file | tee -a "$SQUIRREL_LOG" + goto skip + endif + + # beautify it + set expname = `echo $abiname | sed 's/ /_/g'` + echo $expname | tee -a "$SQUIRREL_LOG" + + echo $file":"$expname >> ${file_list} + +skip: +end + +exit 0 + + +abort: +# +# interruption - not successful completion +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/README b/src/squirrel-1.4/scripts/README new file mode 100644 index 0000000..4c24499 --- /dev/null +++ b/src/squirrel-1.4/scripts/README @@ -0,0 +1,18 @@ + Dir: $SQUIRREL/scripts/ +Date: Fri May 07 12:13:12 BST 1993 + +This directory contains all the scripts required by squirrel, plus a +few others. The following concern the users of squirrel directly: + +squirrel + This is the main script that drives squirrel. It should be + placed in the user's project directory and edited + appropriately. + +packhorse + This is for merging multiple ABI results folders into one, so + that squirrel can process them all in one pass. + +clean + This removes results folders, but only if there appears to be + no files left unprocessed. diff --git a/src/squirrel-1.4/scripts/assemble-error-codes b/src/squirrel-1.4/scripts/assemble-error-codes new file mode 100644 index 0000000..e053328 --- /dev/null +++ b/src/squirrel-1.4/scripts/assemble-error-codes @@ -0,0 +1,17 @@ +0 file not found + The file in the file of file names was not found. + +1 read too short + The read was shorter than the minimum match length. + +2 failed to align and not entered + The read had minimum match length hits with more than one + reading and didn't aligned with any. + +3 failed on entry + The read already exists in the database. + +4 failed to align but entered + The read was to make a join, but after being incorporated into + one contig, it could not be aligned successfully with the other + within the assembly constraints. diff --git a/src/squirrel-1.4/scripts/assemble-files.csh b/src/squirrel-1.4/scripts/assemble-files.csh new file mode 100644 index 0000000..9730d52 --- /dev/null +++ b/src/squirrel-1.4/scripts/assemble-files.csh @@ -0,0 +1,373 @@ +#! /bin/csh -f +# +# assemble-files +# +# Script to assemble data into a database using a file of file names. +# Output from the initial assembly is directed to a file that is +# deleted, hence nothing appears on the screen. The failed files are +# then recompared with the output directed to another file. This +# file is printed then both output files are deleted. +# +# Usage: +# assemble-files project file_of_filenames +# +# 27-May-1992 Set consensus cutoff percentage to 51 for assembly +# 15-Oct-1992 PROJECT was not set to information in databases table! +# Rather - it was set to $1 ("project" name) +# 22-Oct-1992 Should delete busy file for working version +# 05-Jan-1993 Check copying to working version is successful +# 13-Jan-1993 FAILURES_PASS1 is the important one when reporting +# errors, not FAILURES_PASS2 +# 18-May-1993 **** this script will only work with bap version 12.1 or greater **** +# Bap auto assembly now prompts for "Hide alignments?" (after "Permit entry?") +# We should respond "Y" first pass, "N" the second +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +if ( $#argv != 2 ) then + echo "Usage: assemble-files project file_of_filenames" | tee -a "$SQUIRREL_LOG" + exit 2 +endif + +if ( ! -f $2 ) then + echo "assemble-files: File of file names not found" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +# +# Commands used in this script +# +set RM = "/bin/rm -f" +set CP = "/bin/cp" +set MV = "/bin/mv" + + + + +# Directories +if ( $?SQUIRREL_SUB ) then + set TABLES_DIR = $SQUIRREL/tables-$SQUIRREL_SUB +else + set TABLES_DIR = $SQUIRREL/tables +endif + + + + +# +# Gather project infomation from lookup +# +set GET_LOOK_UP = $TABLES_DIR/lookup.csh +set db_info = `$GET_LOOK_UP "$1" $TABLES_DIR/databases.lookup` +if ( $#db_info == 0 ) then + echo assemble-files: No database information for $1 | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +echo "" | tee -a "$SQUIRREL_LOG" +echo Assembling data from file of filenames $2 into database $1 | tee -a "$SQUIRREL_LOG" +echo "" | tee -a "$SQUIRREL_LOG" + +# +# database versions +# +set PROJECT = $db_info[3] +set V_CURRENT = $db_info[4] +set V_PREVIOUS = "1" +set V_WORKING = "X" + +# +# Check for database's existence +# +if (! -e ${PROJECT}.SQ${V_CURRENT}) then + echo assemble-files: Cannot find database ${PROJECT} version ${V_CURRENT} | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +# +# Check database isn't already busy +# +if (-e ${PROJECT}_BUSY${V_CURRENT}) then + echo "assemble-files: Version ${V_CURRENT} of the database ${PROJECT} is currently being used" | tee -a "$SQUIRREL_LOG" + echo " Processing has been suspended - Please try again later" | tee -a "$SQUIRREL_LOG" + echo " (running squirrel again will allow you to resume from this point)" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + + +# +# Delete old working version +# +$RM ${PROJECT}.RL${V_WORKING} +$RM ${PROJECT}.SQ${V_WORKING} +$RM ${PROJECT}.AR${V_WORKING} +$RM ${PROJECT}.CC${V_WORKING} +$RM ${PROJECT}.TG${V_WORKING} +$RM ${PROJECT}_BUSY${V_WORKING} + +# +# Make a copy of the current version +# +# YUK! should check that each copy succeeds +$CP ${PROJECT}.RL${V_CURRENT} ${PROJECT}.RL${V_WORKING} +if ( $status ) then + echo assemble-files: Error copying database file ${PROJECT}.RL${V_CURRENT} | tee -a "$SQUIRREL_LOG" + exit 1 +endif +$CP ${PROJECT}.SQ${V_CURRENT} ${PROJECT}.SQ${V_WORKING} +if ( $status ) then + echo assemble-files: Error copying database file ${PROJECT}.SQ${V_CURRENT} | tee -a "$SQUIRREL_LOG" + exit 1 +endif +$CP ${PROJECT}.AR${V_CURRENT} ${PROJECT}.AR${V_WORKING} +if ( $status ) then + echo assemble-files: Error copying database file ${PROJECT}.AR${V_CURRENT} | tee -a "$SQUIRREL_LOG" + exit 1 +endif +$CP ${PROJECT}.CC${V_CURRENT} ${PROJECT}.CC${V_WORKING} +if ( $status ) then + echo assemble-files: Error copying database file ${PROJECT}.CC${V_CURRENT} | tee -a "$SQUIRREL_LOG" + exit 1 +endif +$CP ${PROJECT}.TG${V_CURRENT} ${PROJECT}.TG${V_WORKING} +if ( $status ) then + echo assemble-files: Error copying database file ${PROJECT}.TG${V_CURRENT} | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +# +# Temporary files for assembly +# +set FAILURES_PASS1 = _fail.$$ +set FAILURES_PASS2 = _fail2.$$ +set ASSEMBLE_LOG = _show.$$ +set REPORT = _show2.$$ + +# +# assembly parameters +# +set DEFAULT = "" +set CUT_OFF = 51 +set VECTOR_SCORE = 20 +set ASSEMBLY_SCORE = 20 +set MAX_READ_PADS = 25 +set MAX_CONTIG_PADS = 25 +set MAX_MISMATCH = 8 + +# +# Determine assembly program +# ?? Why do this? Why not assume $db_info[5] +# +switch ( $db_info[5] ) +case "bap": + #set ASSEMBLE = /nfs/al/pubseq/pubseq/inhouse/sun-nematode/bin/bap + set ASSEMBLE = bap + breaksw +case "dap": + echo assemble-files: Squirrel does not supoport database type $db_info[5] | tee -a "$SQUIRREL_LOG" + exit 1 + ##set ASSEMBLE = /nfs/al/pubseq/pubseq/inhouse/sun-nematode/bin/dap + #set ASSEMBLE = dap + #breaksw +deault: + echo assemble-files: Unknown database type $db_info[5] | tee -a "$SQUIRREL_LOG" + exit 1 +endsw + +# +# ASSEMBLE +# +# ---------------------------------ASSEMBLE--------------------------- +$ASSEMBLE << EOF +3 +y +${PROJECT} +${V_WORKING} + +27 +${DEFAULT} +${CUT_OFF} +${DEFAULT} +${DEFAULT} +${DEFAULT} + +20 +Y +y +Y +$2 +${FAILURES_PASS1} +1 +Y +${ASSEMBLY_SCORE} +${MAX_READ_PADS} +${MAX_CONTIG_PADS} +${MAX_MISMATCH} + +7 +1 +${ASSEMBLE_LOG} + +20 +y +n +y +${FAILURES_PASS1} +${FAILURES_PASS2} +1 +y +${ASSEMBLY_SCORE} +${MAX_READ_PADS} +${MAX_CONTIG_PADS} +${MAX_MISMATCH} + +25 +! + +7 +y + +! +EOF +# =================================ASSEMBLE=========================== + +# +# Check for successful completion +# +if ( $status ) then + echo assemble-files: Error during assembly | tee -a "$SQUIRREL_LOG" + $RM ${PROJECT}.RL${V_WORKING} + $RM ${PROJECT}.SQ${V_WORKING} + $RM ${PROJECT}.AR${V_WORKING} + $RM ${PROJECT}.CC${V_WORKING} + $RM ${PROJECT}.TG${V_WORKING} + exit 1 +endif + +# +# Switch databases +# +# old copy to save copy +$RM ${PROJECT}.RL${V_PREVIOUS} +$RM ${PROJECT}.SQ${V_PREVIOUS} +$RM ${PROJECT}.AR${V_PREVIOUS} +$RM ${PROJECT}.CC${V_PREVIOUS} +$RM ${PROJECT}.TG${V_PREVIOUS} +# keep an old copy of the current version +$MV ${PROJECT}.RL${V_CURRENT} ${PROJECT}.RL${V_PREVIOUS} +$MV ${PROJECT}.SQ${V_CURRENT} ${PROJECT}.SQ${V_PREVIOUS} +$MV ${PROJECT}.AR${V_CURRENT} ${PROJECT}.AR${V_PREVIOUS} +$MV ${PROJECT}.CC${V_CURRENT} ${PROJECT}.CC${V_PREVIOUS} +$MV ${PROJECT}.TG${V_CURRENT} ${PROJECT}.TG${V_PREVIOUS} +# make the new version current +$MV ${PROJECT}.RL${V_WORKING} ${PROJECT}.RL${V_CURRENT} +$MV ${PROJECT}.SQ${V_WORKING} ${PROJECT}.SQ${V_CURRENT} +$MV ${PROJECT}.AR${V_WORKING} ${PROJECT}.AR${V_CURRENT} +$MV ${PROJECT}.CC${V_WORKING} ${PROJECT}.CC${V_CURRENT} +$MV ${PROJECT}.TG${V_WORKING} ${PROJECT}.TG${V_CURRENT} + +# +# Determine assembly failures and successes +# +set temp_in = _temp_in.$$ +set temp_out = _temp_out.$$ +set temp_out2 = _temp_out2.$$ +tr -d ' ' < $2 | sort > ${temp_in} +# no stripping of space needes as done by awk +sort ${FAILURES_PASS1} > ${temp_out} +# Strip out error codes +awk '{print $1;}' ${temp_out} > ${temp_out2} +set expfiles = `cat ${temp_out2}` +set errcodes = `awk '{if (NF==2)print $2;else print -1;}' ${temp_out}` + +while ( $#expfiles ) + + set expname = $expfiles[1] + set expfile = Expfiles/$expfiles[1] + set errcode = $errcodes[1] + + switch(${errcode}) + case 0: + set reason = " : file not found" + breaksw; + case 1: + set reason = " : read too short" + breaksw; + case 2: + set reason = " : failed to align and not entered" + breaksw; + case 3: + set reason = " : failed on entry" + breaksw; + case 4: + set reason = " : failed to align but entered" + breaksw; + default: + set reason = "" #Unknown + breaksw; + endsw + + if ( ${errcode} == 4 ) then + # This actually assembled + echo OK: ${expname}: Assembled $reason | tee -a "$SQUIRREL_LOG" + else + echo Rejected: ${expname}: Did not assemble $reason | tee -a "$SQUIRREL_LOG" + if (-e ${expfile}) echo "PS Did not assemble $reason" >> ${expfile} + endif + + shift expfiles + shift errcodes +end + +# +# Determine passes +# +foreach expfile (`comm -23 ${temp_in} ${temp_out2}`) + echo OK: ${expfile}: Assembled | tee -a "$SQUIRREL_LOG" +end + +# +# Remove temporary files +# +$RM ${temp_in} ${temp_out} ${temp_out2} + +# +# Produce a report +# +cat ${FAILURES_PASS1} >> ${PROJECT}.FAILS +echo Assembly for project ${PROJECT} on `date` >> ${REPORT} +echo In directory `pwd` >> ${REPORT} +echo Input file of file names $2 >> ${REPORT} +echo Parameters used >> ${REPORT} +echo Minimum initial match = ${ASSEMBLY_SCORE} >> ${REPORT} +echo Maximum pads in each reading = ${MAX_READ_PADS} >> ${REPORT} +echo Maximum pads in contig = ${MAX_CONTIG_PADS} >> ${REPORT} +echo Maximum percent mismatch = ${MAX_MISMATCH} >> ${REPORT} + +echo "List of entry failures ">> ${REPORT} +cat ${FAILURES_PASS1} >> ${REPORT} + +echo "Alignments for entry failures " >> ${REPORT} +cat ${ASSEMBLE_LOG} >> ${REPORT} + +lpr ${REPORT} + + +# +# Remove temporary files +# +$RM ${FAILURES_PASS1} ${FAILURES_PASS2} ${ASSEMBLE_LOG} ${REPORT} + +exit 0 + + + +abort: + +# +# interruption - not successful conclusion +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/check-exp-exists.csh b/src/squirrel-1.4/scripts/check-exp-exists.csh new file mode 100644 index 0000000..582c179 --- /dev/null +++ b/src/squirrel-1.4/scripts/check-exp-exists.csh @@ -0,0 +1,85 @@ +#! /bin/csh -f +# +# check-exp-exists +# Checks that experiments don't already exist in project +# directory +# +# Usage: +# check-exp-exists project-directory session_file out_fofn +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +# +# Check usage +# +if ( $#argv != 3 ) then + echo "Usage: check-exp-exists project-directory session_file out_fofn" | tee -a "$SQUIRREL_LOG" + exit 2 +endif + + +if ( ! -d $1 ) then + echo "check-exp-exists: Project directory $1 does not exist" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +if ( ! -e $2 ) then + echo "check-exp-exists: Session file $2 does not exist" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +if ( -e $3 ) then + echo "check-exp-exists: File of file names $3 already exists" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +# +# Save typing if need to change +# +set FAILURES = $1/Failures +set EXPFILES = $1/Expfiles + +# +# Determine experiment file list to process this time +# +set temp_fofn = _temp_fofn +if ( -e ${temp_fofn} ) /bin/rm -f ${temp_fofn} +awk 'BEGIN {FS = ":";} /^#/ {next;} {print $2;}' $2 > ${temp_fofn} + + +echo "" | tee -a "$SQUIRREL_LOG" +echo "Checking files don't alreay exist in $1" | tee -a "$SQUIRREL_LOG" +touch $3 +foreach expname ( `cat ${temp_fofn}` ) + + set file = `awk 'BEGIN {FS=":";} $2 == "'$expname'" { s = $1; } END { print s;}' $2` + + # details for log + echo "**" $expname from $file | tee -a "$SQUIRREL_LOG" + + # Check expname doesn't already exist + if ( -e $1/$expname || -e ${EXPFILES}/$expname || -e ${FAILURES}/$expname ) then + echo Abandoned: ${expname}: Experiment already processed | tee -a "$SQUIRREL_LOG" + goto skip + endif + + echo $expname >> $3 + +skip: +end + +# +# Remove temporary files +# +/bin/rm -f ${temp_fofn} + +exit 0 + +abort: +# +# interruption not normal exit +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/check-quality.csh b/src/squirrel-1.4/scripts/check-quality.csh new file mode 100644 index 0000000..6cb59e0 --- /dev/null +++ b/src/squirrel-1.4/scripts/check-quality.csh @@ -0,0 +1,63 @@ +#! /bin/csh -f +# +# check-quality +# Check a file of file name for quality +# +# Usage: +# create-exp-files fofn_in fofn_out +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +# Usage +if ( $#argv != 2 ) then + echo "Usage: check-quality fofn_in fofn_out" | tee -a "$SQUIRREL_LOG" + exit 2 +endif +if ( ! -e $1 ) then + echo check-quality: file of file names $1 does not exist | tee -a "$SQUIRREL_LOG" + exit 1 +endif +if ( -e $2 ) then + echo check-quality: output file of file names $1 already exists | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +# Programs and scripts +set CHECK_QUALITY = $SQUIRREL/scripts/quality-check.awk + +# +touch $2 + +# Process each file +echo "" | tee -a "$SQUIRREL_LOG" +echo "Checking for quality:" | tee -a "$SQUIRREL_LOG" +foreach file ( `cat $1` ) + + # Do a quality check + set qc = `awk -f $CHECK_QUALITY $file` + if ($qc != "0") then + echo Failed: ${file}: Trace quality | tee -a "$SQUIRREL_LOG" + echo "PS Trace quality" >> ${file} + goto skip + endif + + # update file of file names + echo $file >> $2 + +# skip to here on error +skip: + +end + + +exit 0 + + +abort: +# +# interruption - not successful completion +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/clean b/src/squirrel-1.4/scripts/clean new file mode 100644 index 0000000..0a68759 --- /dev/null +++ b/src/squirrel-1.4/scripts/clean @@ -0,0 +1,48 @@ +#! /bin/csh -f +# +# File: clean +# Version: 1.1 +# +# Author: Simon Dear +# MRC Laboratory of Molecular Biology +# Hills Road +# Cambridge CB2 2QH +# United Kingdom +# +# Created: 19 January 1993 +# Updated: +# +# Description: +# C shell script to remove empty results folders +# +# Usage: +# clean dir [dir...] +# + +set diri = 1 +set dirn = $#argv +while ( $diri <= $dirn ) + set dir = "$argv[$diri]" + + if (! -e "$dir") then + echo $dir does not exist + goto skip + else if (! -d "$dir") then + echo "$dir" is not a directory + goto skip + endif + + # remove directory, but only if no files left + # >150 0.5k disk blocks is arbitrary, but should trap any valid ABI files + set big_files = `find "$dir" -size +150 -print` + if ( $#big_files == 0 ) then + echo Removing directory "$dir" + /bin/rm -r "$dir" + else + echo Not removing directory "$dir" - large files present + endif + +skip: + @ diri ++ +end + diff --git a/src/squirrel-1.4/scripts/clip-exp.awk b/src/squirrel-1.4/scripts/clip-exp.awk new file mode 100644 index 0000000..7e4eb6e --- /dev/null +++ b/src/squirrel-1.4/scripts/clip-exp.awk @@ -0,0 +1,101 @@ +# +# Clip poor quality sequence at the left (5') and right (3') ends +# on basis of sequence content - filter version +# +# Rule: +# To the right of base number SR and to the left of base SL +# everything is good quality before where there are NN +# uncertainties in MM window, in the base range LL..RR +# At the right hand end only, ALWAYS knock back KR bases +# +# St.Louis use NN=2, MM=5, SL=1, LL=1, SR=200, RR=sequence_length, KR=0 +# +# Outputs just the new clip line in experiment file format +# + +BEGIN { + # Up to where NN uncertainties in window of MM bases + NN = 2; + MM = 5; + SL = 100 + SR = 200 + LL = 1; + RR = 450; + KR = 50; +} + +/^\/\// { + slines = 0; +} + +slines==1 { + # sequence may be broken up by white space + for (frag=1;frag<=NF;frag++) seq = seq $frag; +} + + +slines==0 && $1 == "SQ" { + slines = 1; + seq = ""; +} + +{ + print; +} + +END { + lseq = length(seq); + if (RR > lseq) RR = lseq; + if (LL < 1) LL = 1 + lcut = LL - 1; + rcut = RR+1; + if ( NN>0 ) { + # + # clip to the right + # + score = 0; + # calculate default score + for(j=SR-1;j0 && index("ACGTacgt",substr(seq,j,1)) == 0) + score++; + # loop through + for(j=SR; j<=RR; j++) { + if (j>1 && index("ACGTacgt",substr(seq,j-1,1)) == 0) + score--; + if (j+MM-1<=lseq && index("ACGTacgt",substr(seq,j+MM-1,1)) == 0) + score++; + if (score >= NN) { + rcut = j; + break; + } + } + + # + # clip to the left + # + score = 0; + # calculate default score + for(j=SL+1;j>SL-MM+1 && j>0 ; j--) + if(j<=lseq && index("ACGTacgt",substr(seq,j,1)) == 0) + score++; + # loop through + for(j=SL; j>=LL; j--) { + if (j0 && index("ACGTacgt",substr(seq,j-MM+1,1)) == 0) + score++; + if (score >= NN) { + lcut = j; + break; + } + } + } + + # knock back the 3' end + rcut = rcut - KR; + if (rcut <= lcut) rcut = lcut + 1; + + # output + if (lcut >= 1) print "QL " lcut; + if (rcut <= lseq) print "QR " rcut; +} diff --git a/src/squirrel-1.4/scripts/clip-seq-loose.awk b/src/squirrel-1.4/scripts/clip-seq-loose.awk new file mode 100644 index 0000000..ab0782a --- /dev/null +++ b/src/squirrel-1.4/scripts/clip-seq-loose.awk @@ -0,0 +1,120 @@ +# +# Clip poor quality sequence at the left (5') and right (3') ends +# on basis of sequence content +# +# Rule: +# To the right of base number SR and to the left of base SL +# everything is good quality before where there are NN +# uncertainties in MM window, in the base range LL..RR +# At the right hand end only, ALWAYS knock back KR bases +# +# St.Louis use NN=2, MM=5, SL=1, LL=1, SR=200, RR=sequence_length, KR=0 +# +# Outputs just the new clip line in experiment file format +# +# 2-Jun-1992 +# If cutoffs already exist only set if they restrict quality further +# + +BEGIN { + # Up to where NN uncertainties in window of MM bases + NN = 4; + MM = 10; + SL = 50; + SR = 150; + LL = 1; + RR = 450; + KR = 0; + # don't change + QL = -1; + QR = -1; +} + +/^\/\// { + slines = 0; +} + +slines==1 { + # sequence may be broken up by white space + for (frag=1;frag<=NF;frag++) seq = seq $frag; +} + + +slines==0 && $1 == "SQ" { + slines = 1; + seq = ""; +} + +slines==0 && $1 == "QL" { + QL = $2; +} + +slines==0 && $1 == "QR" { + QR = $2; +} + +END { + lseq = length(seq); + if (RR > lseq) RR = lseq; + if (LL < 1) LL = 1 + lcut = LL - 1; + rcut = RR+1; + if ( NN>0 ) { + # + # clip to the right + # + score = 0; + # calculate default score + for(j=SR-1;j0 && index("ACGTacgt",substr(seq,j,1)) == 0) + score++; + # loop through + for(j=SR; j<=RR; j++) { + if (j>1 && index("ACGTacgt",substr(seq,j-1,1)) == 0) + score--; + if (j+MM-1<=lseq && index("ACGTacgt",substr(seq,j+MM-1,1)) == 0) + score++; + if (score >= NN) { + rcut = j; + break; + } + } + + # + # clip to the left + # + score = 0; + # calculate default score + for(j=SL+1;j>SL-MM+1 && j>0 ; j--) + if(j<=lseq && index("ACGTacgt",substr(seq,j,1)) == 0) + score++; + # loop through + for(j=SL; j>=LL; j--) { + if (j0 && index("ACGTacgt",substr(seq,j-MM+1,1)) == 0) + score++; + if (score >= NN) { + lcut = j; + break; + } + } + } + + # knock back the 3' end + rcut = rcut - KR; + if (rcut <= lcut) rcut = lcut + 1; + + # Determine old values + if (QR<0) QR = lseq+1; + if (QL<0) QL = 0; + # Constrain new values to old values + if (lcut >= QR) lcut = QR - 1; + if (rcut <= QL) rcut = QL + 1; + # Print out new values (if they differ) + if ( (lcut > QL) || (rcut < QR) ) { + print "CC Quality clipping by clip-seq-loose.awk"; + if (lcut > QL) print "QL " lcut; + if (rcut < QR) print "QR " rcut; + } +} diff --git a/src/squirrel-1.4/scripts/clip-seq.awk b/src/squirrel-1.4/scripts/clip-seq.awk new file mode 100644 index 0000000..971684c --- /dev/null +++ b/src/squirrel-1.4/scripts/clip-seq.awk @@ -0,0 +1,120 @@ +# +# Clip poor quality sequence at the left (5') and right (3') ends +# on basis of sequence content +# +# Rule: +# To the right of base number SR and to the left of base SL +# everything is good quality before where there are NN +# uncertainties in MM window, in the base range LL..RR +# At the right hand end only, ALWAYS knock back KR bases +# +# St.Louis use NN=2, MM=5, SL=1, LL=1, SR=200, RR=sequence_length, KR=0 +# +# Outputs just the new clip line in experiment file format +# +# 2-Jun-1992 +# If cutoffs already exist only set if they restrict quality further +# + +BEGIN { + # Up to where NN uncertainties in window of MM bases + NN = 2; + MM = 5; + SL = 100 + SR = 200 + LL = 1; + RR = 450; + KR = 50; + # don't change + QL = -1; + QR = -1; +} + +/^\/\// { + slines = 0; +} + +slines==1 { + # sequence may be broken up by white space + for (frag=1;frag<=NF;frag++) seq = seq $frag; +} + + +slines==0 && $1 == "SQ" { + slines = 1; + seq = ""; +} + +slines==0 && $1 == "QL" { + QL = $2; +} + +slines==0 && $1 == "QR" { + QR = $2; +} + +END { + lseq = length(seq); + if (RR > lseq) RR = lseq; + if (LL < 1) LL = 1 + lcut = LL - 1; + rcut = RR+1; + if ( NN>0 ) { + # + # clip to the right + # + score = 0; + # calculate default score + for(j=SR-1;j0 && index("ACGTacgt",substr(seq,j,1)) == 0) + score++; + # loop through + for(j=SR; j<=RR; j++) { + if (j>1 && index("ACGTacgt",substr(seq,j-1,1)) == 0) + score--; + if (j+MM-1<=lseq && index("ACGTacgt",substr(seq,j+MM-1,1)) == 0) + score++; + if (score >= NN) { + rcut = j; + break; + } + } + + # + # clip to the left + # + score = 0; + # calculate default score + for(j=SL+1;j>SL-MM+1 && j>0 ; j--) + if(j<=lseq && index("ACGTacgt",substr(seq,j,1)) == 0) + score++; + # loop through + for(j=SL; j>=LL; j--) { + if (j0 && index("ACGTacgt",substr(seq,j-MM+1,1)) == 0) + score++; + if (score >= NN) { + lcut = j; + break; + } + } + } + + # knock back the 3' end + rcut = rcut - KR; + if (rcut <= lcut) rcut = lcut + 1; + + # Determine old values + if (QR<0) QR = lseq+1; + if (QL<0) QL = 0; + # Constrain new values to old values + if (lcut >= QR) lcut = QR - 1; + if (rcut <= QL) rcut = QL + 1; + # Print out new values (if they differ) + if ( (lcut > QL) || (rcut < QR) ) { + print "CC Quality clipping by squirrel 8-Jun-1992"; + if (lcut > QL) print "QL " lcut; + if (rcut < QR) print "QR " rcut; + } +} diff --git a/src/squirrel-1.4/scripts/do-it-no-assembly.csh b/src/squirrel-1.4/scripts/do-it-no-assembly.csh new file mode 100644 index 0000000..f59cfff --- /dev/null +++ b/src/squirrel-1.4/scripts/do-it-no-assembly.csh @@ -0,0 +1,479 @@ +#! /bin/csh -f +# +# do_it +# Process a batch work of readings +# +# Usage: +# do_it transfer_directory project +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + + +# Usage +if ( $#argv != 2 ) then + echo "Usage: do-it transfer_directory project" | tee -a "$SQUIRREL_LOG" + exit 2 +endif + + +set PROJECT = $2 +set transfer_directory = "$1" + +if ( ! -d "$transfer_directory" ) then + echo "do-it: Bad folder machine or date" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + + + +# Directories +if ( $?SQUIRREL_SUB ) then + set TABLES_DIR = $SQUIRREL/tables-$SQUIRREL_SUB +else + set TABLES_DIR = $SQUIRREL/tables +endif + + +# Programs and scripts +set ABI_SESS_FILE = $SQUIRREL/scripts/ABI-sess-file.csh +set EDIT_FILES = $SQUIRREL/scripts/edit-session-file.csh +set CHECK_FILES = $SQUIRREL/scripts/check-exp-exists.csh +set ABI_EXP_FILES = $SQUIRREL/scripts/ABI-exp-files.csh +set BUILD_EXP_FILES = $SQUIRREL/scripts/embellish-exp-files.csh +set QUALITY_CHECK = $SQUIRREL/scripts/check-quality.csh +set VECTOR_EXCISE = $SQUIRREL/scripts/excise-vector.csh +set TRANSFER_FILES = $SQUIRREL/scripts/transfer-files.csh +set ASSEMBLE_FILES = $SQUIRREL/scripts/assemble-files.csh +set SCREEN_FILES = $SQUIRREL/scripts/screen-against-vector.csh +set BACKUP_DIR = $SQUIRREL/scripts/tidy-files.csh +set LOG_TO_SUMMARY = $SQUIRREL/scripts/log-to-summary.awk + +# +# Gather information about this project +# +set GET_LOOK_UP = $TABLES_DIR/lookup.csh +set db_info = `$GET_LOOK_UP $PROJECT $TABLES_DIR/databases.lookup` +if ( $#db_info == 0 ) then + echo do-it: no database information for $PROJECT + exit 2 +endif +# Check things look ok +if ( ! -e ~$db_info[6]/$db_info[2]/$db_info[3].SQ$db_info[4] ) then + echo do-it: Cannot find database ~$db_info[6]/$db_info[2]/$db_info[3] version $db_info[4] + exit 2 +endif +# Check owner ok +if ( $db_info[6] != $USER ) then + echo do-it: User $USER not registered to use database ~$db_info[6]/$db_info[2]/$db_info[3] version $db_info[4] + exit 2 +endif + + +# +# Go to where all the action is +# +cd "$transfer_directory" + + +# +# Determine which pass this is +# +set pass_file = .squirrel +if (! -e ${pass_file}) then + set last_pass = 0 + set last_phase = completed + touch ${pass_file} +else + set last_pass = `tail -1 ${pass_file} | awk '/^Pass/ {pass = $2;} END {print pass;}'` + set last_phase = `tail -1 ${pass_file} | awk '/^Pass/ {phase = $4;} END {print phase;}'` +endif +@ pass_count = $last_pass + 1 + +set resume = 0 + +if ( "$last_phase" != "completed" ) then + + switch ( "$last_phase" ) + case started: + set phase = "log file creation"; breaksw + case session: + set phase = "session file creation"; breaksw + case edited: + set phase = "session file editing"; breaksw + case checked: + set phase = "checking experiment files don't already exist"; breaksw + case created: + set phase = "experiment files creation"; breaksw + case embellished: + set phase = "experiment files embellishment"; breaksw + case quality: + set phase = "quality checking"; breaksw + case excised: + set phase = "vector removal"; breaksw + case screened: + set phase = "screen against vector"; breaksw + case transferred: + set phase = "file transfer"; breaksw + case assembled: + set phase = "sequence assembly"; breaksw + case reported: + set phase = "reporting"; breaksw + case archived: + set phase = "backing up files"; breaksw + default: + set phase = "***problem***" ; breaksw + endsw + + echo "***" + echo "*** WARNING\!\!\!" + echo "***" + echo "*** SQUIRREL did not successfully complete the last time it was" + echo "*** run on this directory" + echo "***" + echo "" + echo "Last phase successfully completed: $phase" +repeat: + echo -n "Do you want to resume the previous pass? " + set reply = $< + switch ( $reply ) + case [yY]*: + set pass_count = $last_pass + set resume = 1; + breaksw + case [nN]*: + set last_phase = "completed" + breaksw + default: + echo "please reply yes or no" + goto repeat + endsw + echo "" + echo "" + +endif + + +# +# START +# +setenv SQUIRREL_LOG "$transfer_directory"/log.Pass.${pass_count} + + +# +# Files and directories +# +# WARNING : the following line makes assumptions about directory names +# +# YUK! YUK! YUK! the following line is aweful +set temp = ( $1 ) +set session = $temp[2]$temp[3].${pass_count} +set sessfile = sess.$session +set sessfile2 = sess.$session.2 +set filenames = fn.$session +set filenames2 = fn.$session.2 +set filenames3 = fn.$session.3 +set filenames4 = fn.$session.4 + +# +# PHASE 0: Start log file +# +if ( $last_phase == "completed" ) then + if ( -e "$SQUIRREL_LOG" ) /bin/rm -f "$SQUIRREL_LOG" + touch "$SQUIRREL_LOG" + + # log this phase + set last_phase = started + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + +# +# Add messages to log +# +if ( $resume == 0) then + echo SQUIRREL Version 1.4 | tee -a "$SQUIRREL_LOG" + echo "" | tee -a "$SQUIRREL_LOG" + echo Processing "$transfer_directory" | tee -a "$SQUIRREL_LOG" + echo Project $PROJECT | tee -a "$SQUIRREL_LOG" + echo Started at `date` | tee -a "$SQUIRREL_LOG" + echo Pass ${pass_count} | tee -a "$SQUIRREL_LOG" +else + echo Resumed at `date` | tee -a "$SQUIRREL_LOG" + echo Pass ${pass_count} - Phase $phase | tee -a "$SQUIRREL_LOG" +endif + +# +# Create session file files +# +if ( $last_phase == "started" ) then + /bin/rm -f $sessfile + $ABI_SESS_FILE $sessfile + if ($status != 0) then + echo "do-it: Error while creating session file" | tee -a "$SQUIRREL_LOG" + exit 1 + endif + + # log this phase + set last_phase = session + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + +# +# Allow the user to edit the session file to suit themselves +# +if ( $last_phase == "session" ) then + /bin/rm -f $sessfile2 + $EDIT_FILES $sessfile $sessfile2 + if ( $status ) then + echo "do-it: Error while editing session file" | tee -a "$SQUIRREL_LOG" + exit 1 + endif + /bin/mv $sessfile2 $sessfile + + # log this phase + set last_phase = edited + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + +# +# Filter out experiments already processed +# +if ( $last_phase == "edited" ) then + /bin/rm -f $filenames + $CHECK_FILES ~$db_info[6]/$db_info[2] $sessfile $filenames + if ( $status ) then + echo "do-it: Error while checking for previous processing" | tee -a "$SQUIRREL_LOG" + exit 1 + endif + + # log this phase + set last_phase = checked + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + +# +# Create experiment files from session file +# +if ( $last_phase == "checked" ) then + if ( `wc $filenames | awk '{print $2;}'` > 0 ) then + /bin/rm -f $filenames2 + $ABI_EXP_FILES $sessfile $filenames $filenames2 + if ($status != 0) then + echo "do-it: Error while creating experiment files" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + /bin/mv -f $filenames2 $filenames + else + echo do-it: Skipping creation of experiment files | tee -a "$SQUIRREL_LOG" + endif + + # log this phase + set last_phase = created + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + +# +# Fill out the details on the experiment files +# +if ( $last_phase == "created" ) then + if ( `wc $filenames | awk '{print $2;}'` > 0 ) then + /bin/rm -f $filenames2 + $BUILD_EXP_FILES $filenames $filenames2 + if ($status != 0) then + echo "do-it: Error while building experiment files" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + /bin/mv $filenames2 $filenames + else + echo do-it: Skipping building of experiment files | tee -a "$SQUIRREL_LOG" + endif + + # log this phase + set last_phase = embellished + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + +# +# Remove poor quality reads +# +if ( $last_phase == "embellished") then + if ( `wc $filenames | awk '{print $2;}'` > 0 ) then + /bin/rm -f $filenames2 + $QUALITY_CHECK $filenames $filenames2 + if ($status != 0) then + echo "do-it: Error while checking quality of reads" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + else + echo do-it: Skipping quality check of files | tee -a "$SQUIRREL_LOG" + /bin/cp $filenames $filenames2 + endif + + # log this phase + set last_phase = quality + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + +# +# Remove vector sequence +# +if ( $last_phase == "quality" ) then + if ( `wc $filenames2 | awk '{print $2;}'` > 0 ) then + /bin/rm -f $filenames3 + $VECTOR_EXCISE $filenames2 $filenames3 + if ($status != 0) then + echo "do-it: Error while excising vectors from files" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + else + echo do-it: Skipping excising of vector from files | tee -a "$SQUIRREL_LOG" + /bin/cp $filenames2 $filenames3 + endif + + # log this phase + set last_phase = excised + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + + + + + +# +# Screen against vector +# +if ( $last_phase == "excised" ) then + if ( `wc $filenames3 | awk '{print $2;}'` > 0 ) then + /bin/rm -f $filenames4 + $SCREEN_FILES $filenames3 $filenames4 + if ($status != 0) then + echo "do-it: Error while screening files" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + else + echo do-it: Skipping screening of vector from files | tee -a "$SQUIRREL_LOG" + /bin/cp $filenames3 $filenames4 + endif + + # log this phase + set last_phase = screened + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + + + + + +# +# Transfer files - some reformating and converting may occur +# NOTE: The second $filenames is in ~$db_info[6]/$db_info[2] directory +# +if ( $last_phase == screened ) then + if ( `wc $filenames | awk '{print $2;}'` > 0 ) then + /bin/rm -f ~$db_info[6]/$db_info[2]/$filenames + $TRANSFER_FILES ~$db_info[6]/$db_info[2] $filenames $filenames + if ($status != 0) then + echo "do-it: Error while transferring files" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + else + echo do-it: Skipping transfer of files | tee -a "$SQUIRREL_LOG" + endif + + # log this phase + set last_phase = transferred + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + + +# +# Do the assembly +# +if ( $last_phase == transferred ) then + pushd ~$db_info[6]/$db_info[2] + if ( `wc $filenames | awk '{print $2;}'` > 0 ) then + echo do-it: Skipping assembly of files | tee -a "$SQUIRREL_LOG" + foreach expfile (`cat $filenames`) + echo OK: ${expfile}: Not yet assembled | tee -a "$SQUIRREL_LOG" + end + else + echo do-it: Skipping assembly of files | tee -a "$SQUIRREL_LOG" + endif + popd + + # log this phase + set last_phase = assembled + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + +# +# Generate log to tell us what has happened with all the files this pass +# +if ( $last_phase == assembled ) then + pushd ~$db_info[6]/$db_info[2] + if (! -e Logs) mkdir Logs + set session_log = log.$session + awk -f $LOG_TO_SUMMARY "$SQUIRREL_LOG" > Logs/$session_log + /bin/cp Logs/$session_log "$transfer_directory" + lpr Logs/$session_log + popd + + # log this phase + set last_phase = reported + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + +# +# Back up +# +if ( $last_phase == reported ) then + $BACKUP_DIR $session_log + if ($status != 0) then + echo "do-it: Error while tidying up files in transfer directory" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + + # log this phase + set last_phase = archived + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + +# +# Remove temporary files +# +/bin/rm -f $sessfile $sessfile2 $filenames $filenames2 $filenames3 $filenames4 + + +# +# Completed successfully +# + +# log this phase +set last_phase = completed +echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} + +echo Completed at `date` | tee -a "$SQUIRREL_LOG" + +exit 0 + + +abort: +# +# Interruption not normal termination +# + +if ( $?SQUIRREL_LOG ) then + echo Aborted at `date` | tee -a "$SQUIRREL_LOG" +else + echo Aborted at `date` +endif + +exit 1 diff --git a/src/squirrel-1.4/scripts/do-it.csh b/src/squirrel-1.4/scripts/do-it.csh new file mode 100644 index 0000000..6adca75 --- /dev/null +++ b/src/squirrel-1.4/scripts/do-it.csh @@ -0,0 +1,480 @@ +#! /bin/csh -f +# +# do_it +# Process a batch work of readings +# +# Usage: +# do_it transfer_directory project +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + + +# Usage +if ( $#argv != 2 ) then + echo "Usage: do-it transfer_directory project" | tee -a "$SQUIRREL_LOG" + exit 2 +endif + + +set PROJECT = $2 +set transfer_directory = "$1" + +if ( ! -d "$transfer_directory" ) then + echo "do-it: Bad folder machine or date" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + + + +# Directories +if ( $?SQUIRREL_SUB ) then + set TABLES_DIR = $SQUIRREL/tables-$SQUIRREL_SUB +else + set TABLES_DIR = $SQUIRREL/tables +endif + + +# Programs and scripts +set ABI_SESS_FILE = $SQUIRREL/scripts/ABI-sess-file.csh +set EDIT_FILES = $SQUIRREL/scripts/edit-session-file.csh +set CHECK_FILES = $SQUIRREL/scripts/check-exp-exists.csh +set ABI_EXP_FILES = $SQUIRREL/scripts/ABI-exp-files.csh +set BUILD_EXP_FILES = $SQUIRREL/scripts/embellish-exp-files.csh +set QUALITY_CHECK = $SQUIRREL/scripts/check-quality.csh +set VECTOR_EXCISE = $SQUIRREL/scripts/excise-vector.csh +set TRANSFER_FILES = $SQUIRREL/scripts/transfer-files.csh +set ASSEMBLE_FILES = $SQUIRREL/scripts/assemble-files.csh +set SCREEN_FILES = $SQUIRREL/scripts/screen-against-vector.csh +set BACKUP_DIR = $SQUIRREL/scripts/tidy-files.csh +set LOG_TO_SUMMARY = $SQUIRREL/scripts/log-to-summary.awk + +# +# Gather information about this project +# +set GET_LOOK_UP = $TABLES_DIR/lookup.csh +set db_info = `$GET_LOOK_UP $PROJECT $TABLES_DIR/databases.lookup` +if ( $#db_info == 0 ) then + echo do-it: no database information for $PROJECT + exit 2 +endif +# Check things look ok +if ( ! -e ~$db_info[6]/$db_info[2]/$db_info[3].SQ$db_info[4] ) then + echo do-it: Cannot find database ~$db_info[6]/$db_info[2]/$db_info[3] version $db_info[4] + exit 2 +endif +# Check owner ok +if ( $db_info[6] != $USER ) then + echo do-it: User $USER not registered to use database ~$db_info[6]/$db_info[2]/$db_info[3] version $db_info[4] + exit 2 +endif + + +# +# Go to where all the action is +# +cd "$transfer_directory" + + +# +# Determine which pass this is +# +set pass_file = .squirrel +if (! -e ${pass_file}) then + set last_pass = 0 + set last_phase = completed + touch ${pass_file} +else + set last_pass = `tail -1 ${pass_file} | awk '/^Pass/ {pass = $2;} END {print pass;}'` + set last_phase = `tail -1 ${pass_file} | awk '/^Pass/ {phase = $4;} END {print phase;}'` +endif +@ pass_count = $last_pass + 1 + +set resume = 0 + +if ( "$last_phase" != "completed" ) then + + switch ( "$last_phase" ) + case started: + set phase = "log file creation"; breaksw + case session: + set phase = "session file creation"; breaksw + case edited: + set phase = "session file editing"; breaksw + case checked: + set phase = "checking experiment files don't already exist"; breaksw + case created: + set phase = "experiment files creation"; breaksw + case embellished: + set phase = "experiment files embellishment"; breaksw + case quality: + set phase = "quality checking"; breaksw + case excised: + set phase = "vector removal"; breaksw + case screened: + set phase = "screen against vector"; breaksw + case transferred: + set phase = "file transfer"; breaksw + case assembled: + set phase = "sequence assembly"; breaksw + case reported: + set phase = "reporting"; breaksw + case archived: + set phase = "backing up files"; breaksw + default: + set phase = "***problem***" ; breaksw + endsw + + echo "***" + echo "*** WARNING\!\!\!" + echo "***" + echo "*** SQUIRREL did not successfully complete the last time it was" + echo "*** run on this directory" + echo "***" + echo "" + echo "Last phase successfully completed: $phase" +repeat: + echo -n "Do you want to resume the previous pass? " + set reply = $< + switch ( $reply ) + case [yY]*: + set pass_count = $last_pass + set resume = 1; + breaksw + case [nN]*: + set last_phase = "completed" + breaksw + default: + echo "please reply yes or no" + goto repeat + endsw + echo "" + echo "" + +endif + + +# +# START +# +setenv SQUIRREL_LOG "$transfer_directory"/log.Pass.${pass_count} + + +# +# Files and directories +# +# WARNING : the following line makes assumptions about directory names +# +# YUK! YUK! YUK! the following line is aweful +set temp = ( $1 ) +set session = $temp[2]$temp[3].${pass_count} +set sessfile = sess.$session +set sessfile2 = sess.$session.2 +set filenames = fn.$session +set filenames2 = fn.$session.2 +set filenames3 = fn.$session.3 +set filenames4 = fn.$session.4 + +# +# PHASE 0: Start log file +# +if ( $last_phase == "completed" ) then + if ( -e "$SQUIRREL_LOG" ) /bin/rm -f "$SQUIRREL_LOG" + touch "$SQUIRREL_LOG" + + # log this phase + set last_phase = started + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + +# +# Add messages to log +# +if ( $resume == 0) then + echo SQUIRREL Version 1.4 | tee -a "$SQUIRREL_LOG" + echo "" | tee -a "$SQUIRREL_LOG" + echo Processing "$transfer_directory" | tee -a "$SQUIRREL_LOG" + echo Project $PROJECT | tee -a "$SQUIRREL_LOG" + echo Started at `date` | tee -a "$SQUIRREL_LOG" + echo Pass ${pass_count} | tee -a "$SQUIRREL_LOG" +else + echo Resumed at `date` | tee -a "$SQUIRREL_LOG" + echo Pass ${pass_count} - Phase $phase | tee -a "$SQUIRREL_LOG" +endif + +# +# Create session file files +# +if ( $last_phase == "started" ) then + /bin/rm -f $sessfile + $ABI_SESS_FILE $sessfile + if ($status != 0) then + echo "do-it: Error while creating session file" | tee -a "$SQUIRREL_LOG" + exit 1 + endif + + # log this phase + set last_phase = session + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + +# +# Allow the user to edit the session file to suit themselves +# +if ( $last_phase == "session" ) then + /bin/rm -f $sessfile2 + $EDIT_FILES $sessfile $sessfile2 + if ( $status ) then + echo "do-it: Error while editing session file" | tee -a "$SQUIRREL_LOG" + exit 1 + endif + /bin/mv $sessfile2 $sessfile + + # log this phase + set last_phase = edited + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + +# +# Filter out experiments already processed +# +if ( $last_phase == "edited" ) then + /bin/rm -f $filenames + $CHECK_FILES ~$db_info[6]/$db_info[2] $sessfile $filenames + if ( $status ) then + echo "do-it: Error while checking for previous processing" | tee -a "$SQUIRREL_LOG" + exit 1 + endif + + # log this phase + set last_phase = checked + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + +# +# Create experiment files from session file +# +if ( $last_phase == "checked" ) then + if ( `wc $filenames | awk '{print $2;}'` > 0 ) then + /bin/rm -f $filenames2 + $ABI_EXP_FILES $sessfile $filenames $filenames2 + if ($status != 0) then + echo "do-it: Error while creating experiment files" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + /bin/mv -f $filenames2 $filenames + else + echo do-it: Skipping creation of experiment files | tee -a "$SQUIRREL_LOG" + endif + + # log this phase + set last_phase = created + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + +# +# Fill out the details on the experiment files +# +if ( $last_phase == "created" ) then + if ( `wc $filenames | awk '{print $2;}'` > 0 ) then + /bin/rm -f $filenames2 + $BUILD_EXP_FILES $filenames $filenames2 + if ($status != 0) then + echo "do-it: Error while building experiment files" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + /bin/mv $filenames2 $filenames + else + echo do-it: Skipping building of experiment files | tee -a "$SQUIRREL_LOG" + endif + + # log this phase + set last_phase = embellished + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + +# +# Remove poor quality reads +# +if ( $last_phase == "embellished") then + if ( `wc $filenames | awk '{print $2;}'` > 0 ) then + /bin/rm -f $filenames2 + $QUALITY_CHECK $filenames $filenames2 + if ($status != 0) then + echo "do-it: Error while checking quality of reads" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + else + echo do-it: Skipping quality check of files | tee -a "$SQUIRREL_LOG" + /bin/cp $filenames $filenames2 + endif + + # log this phase + set last_phase = quality + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + +# +# Remove vector sequence +# +if ( $last_phase == "quality" ) then + if ( `wc $filenames2 | awk '{print $2;}'` > 0 ) then + /bin/rm -f $filenames3 + $VECTOR_EXCISE $filenames2 $filenames3 + if ($status != 0) then + echo "do-it: Error while excising vectors from files" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + else + echo do-it: Skipping excising of vector from files | tee -a "$SQUIRREL_LOG" + /bin/cp $filenames2 $filenames3 + endif + + # log this phase + set last_phase = excised + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + + + + + +# +# Screen against vector +# +if ( $last_phase == "excised" ) then + if ( `wc $filenames3 | awk '{print $2;}'` > 0 ) then + /bin/rm -f $filenames4 + $SCREEN_FILES $filenames3 $filenames4 + if ($status != 0) then + echo "do-it: Error while screening files" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + else + echo do-it: Skipping screening of vector from files | tee -a "$SQUIRREL_LOG" + /bin/cp $filenames3 $filenames4 + endif + + # log this phase + set last_phase = screened + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + + + + + +# +# Transfer files - some reformating and converting may occur +# NOTE: The second $filenames is in ~$db_info[6]/$db_info[2] directory +# +if ( $last_phase == screened ) then + if ( `wc $filenames | awk '{print $2;}'` > 0 ) then + /bin/rm -f ~$db_info[6]/$db_info[2]/$filenames + $TRANSFER_FILES ~$db_info[6]/$db_info[2] $filenames $filenames + if ($status != 0) then + echo "do-it: Error while transferring files" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + else + echo do-it: Skipping transfer of files | tee -a "$SQUIRREL_LOG" + endif + + # log this phase + set last_phase = transferred + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + + +# +# Do the assembly +# +if ( $last_phase == transferred ) then + pushd ~$db_info[6]/$db_info[2] + if ( `wc $filenames | awk '{print $2;}'` > 0 ) then + $ASSEMBLE_FILES $PROJECT $filenames + if ($status != 0) then + echo "do-it: Error while assembling files into database" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + else + echo do-it: Skipping assembly of files | tee -a "$SQUIRREL_LOG" + endif + popd + + # log this phase + set last_phase = assembled + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + +# +# Generate log to tell us what has happened with all the files this pass +# +if ( $last_phase == assembled ) then + pushd ~$db_info[6]/$db_info[2] + if (! -e Logs) mkdir Logs + set session_log = log.$session + awk -f $LOG_TO_SUMMARY "$SQUIRREL_LOG" > Logs/$session_log + /bin/cp Logs/$session_log "$transfer_directory" + lpr Logs/$session_log + popd + + # log this phase + set last_phase = reported + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + +# +# Back up +# +if ( $last_phase == reported ) then + $BACKUP_DIR $session_log + if ($status != 0) then + echo "do-it: Error while tidying up files in transfer directory" | tee -a "$SQUIRREL_LOG" + exit 2 + endif + + # log this phase + set last_phase = archived + echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} +endif + + +# +# Remove temporary files +# +/bin/rm -f $sessfile $sessfile2 $filenames $filenames2 $filenames3 $filenames4 + + +# +# Completed successfully +# + +# log this phase +set last_phase = completed +echo Pass ${pass_count} : ${last_phase} `date` >> ${pass_file} + +echo Completed at `date` | tee -a "$SQUIRREL_LOG" + +exit 0 + + +abort: +# +# Interruption not normal termination +# + +if ( $?SQUIRREL_LOG ) then + echo Aborted at `date` | tee -a "$SQUIRREL_LOG" +else + echo Aborted at `date` +endif + +exit 1 diff --git a/src/squirrel-1.4/scripts/edit-session-file.csh b/src/squirrel-1.4/scripts/edit-session-file.csh new file mode 100644 index 0000000..97c7753 --- /dev/null +++ b/src/squirrel-1.4/scripts/edit-session-file.csh @@ -0,0 +1,92 @@ +#! /bin/csh -f +# +# edit-session-file +# +# Allow a session file to be edited, then parse it for editing +# mistakes. +# +# Usage: +# edit-session-file fof_in fof_out +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +# Usage +if ( $#argv != 2 ) then + echo Usage: edit-session-file fof_in fof_out + exit 2 +endif + +if ( ! -f $1 ) then + echo edit-session-file: file of file names $1 does not exist. | tee -a "$SQUIRREL_LOG" + exit 1 +endif +if ( -e $2 ) then + echo exit-session-file: file $2 exists. | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +# +# Where the wild things are +# +set PARSE_EDIT = $SQUIRREL/scripts/parse-session-file.awk + + +# +# Temporary files +# +set sess_edit = _session.$$ +set parse_out = _parse.$$ +set spacer = _spacer.$$ + +# +# Make a copy +# +/bin/cp $1 ${sess_edit} + +# +# Allow the user to edit the file +# +$EDITOR ${sess_edit} + +# +# Create a spacer - DO NOT CHANGE THIS!!!! +# +cat << EOF > ${spacer} +##PASS-2## +EOF + +# +# Parse the edited file for errors +# +awk -f $PARSE_EDIT $1 ${spacer} ${sess_edit} > ${parse_out} + +# +# Report errors +# +echo "" | tee -a "$SQUIRREL_LOG" +echo Parsing edited file... | tee -a "$SQUIRREL_LOG" +grep -v '^OUT>' ${parse_out} | tee -a "$SQUIRREL_LOG" + +# +# Gather clean lines +# +awk '/^OUT>/ {print substr($0,length($1)+2);}' ${parse_out} > $2 + +# +# Tidy up +# +/bin/rm -f ${sess_edit} ${parse_out} ${spacer} + + + +exit 0 + + +abort: +# +# interruption not normal exit +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/embellish-exp-files.csh b/src/squirrel-1.4/scripts/embellish-exp-files.csh new file mode 100644 index 0000000..171a15a --- /dev/null +++ b/src/squirrel-1.4/scripts/embellish-exp-files.csh @@ -0,0 +1,201 @@ +#! /bin/csh -f +# +# embellish-exp-files +# Fill in the details of an experiment file once the machine specific bits +# have been done. +# +# Usage: +# embellish-exp-files fofn_in fofn_out +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +# Usage +if ( $#argv != 2 ) then + echo Usage: embellish-exp-files fofn_in fofn_out | tee -a "$SQUIRREL_LOG" + exit 2 +endif +if ( ! -e $1 ) then + echo embellish-exp-files: File of file names $1 does not exist | tee -a "$SQUIRREL_LOG" + exit 1 +endif +if ( -e $2 ) then + echo embellish-exp-files: Output file of file names $2 already exists | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +# create output file of file names +touch $2 + +# Directories +if ( $?SQUIRREL_SUB ) then + set TABLES_DIR = $SQUIRREL/tables-$SQUIRREL_SUB +else + set TABLES_DIR = $SQUIRREL/tables +endif + +# Programs and scripts +set GET_MACH_SEQ = $SQUIRREL/bin/expGetSeq +set GET_CLIP_SEQ = $SQUIRREL/scripts/clip-seq.awk +set GET_LOOK_UP = $TABLES_DIR/lookup.csh +set MAKE_SCF_FILE = $SQUIRREL/bin/makeSCF + + + + + +# Process each file +echo "" | tee -a "$SQUIRREL_LOG" +echo "Embellishing experiment file for:" | tee -a "$SQUIRREL_LOG" +foreach file ( `cat $1` ) + + echo $file | tee -a "$SQUIRREL_LOG" + + if ( ! -e $file ) then + echo Abandoned: ${file}: Experiment file missing | tee -a "$SQUIRREL_LOG" + goto skip + endif + + # check not embellished previously (look for OP) + set operator = `awk '/^OP/ {$1="";line = substr($0,2);} END {print line;}' $file` + if ( $operator != "" ) then + # This file has been embellished already - clean it + set tempfile = _temp.exp + /bin/mv -f $file $tempfile + sed '/^OP/,$d' $tempfile > $file + endif + + # beautify it + set expname = `awk '/^EN/ {$1="";line = substr($0,2);} END {print line;}' $file` + if ( $expname == "" ) set expname = $file + + # specify file names + set expfile = $expname + set tracefile = `awk '/^LN/ {$1="";line = substr($0,2);} END {print line;}' $file` + if ( $tracefile == "" ) set tracefile = ${expfile}SCF + + # include obvious information + # OP must always be the first embellished record + echo "OP `whoami`" >> $expfile + + # gather information from lookup files + + # information on subclones/templates + set template = `$GET_LOOK_UP $expname $TABLES_DIR/subclones.lookup` + if ( $#template == 0 ) then + echo Abandoned: ${expfile}: No template information for $expname | tee -a "$SQUIRREL_LOG" + goto skip + endif + + # information on clones and cloning vectors + set clone = `$GET_LOOK_UP $template[2] $TABLES_DIR/clone-types.lookup` + if ( $#clone == 0 ) then + echo Abandoned: ${expfile}: No clone information for $template[2] | tee -a "$SQUIRREL_LOG" + goto skip + endif + + # information on sequencing clone sites + set subclone = `$GET_LOOK_UP $template[5] $TABLES_DIR/seqclone-sites.lookup` + if ( $#subclone == 0 ) then + echo Abandoned: ${expfile}: No cloning site information for $template[5] | tee -a "$SQUIRREL_LOG" + goto skip + endif + + # information on sequencing vector sequence files + set scfile = `$GET_LOOK_UP $template[5] $TABLES_DIR/vector-seqfiles.lookup` + if ( $#scfile == 0 ) then + echo Abandoned: ${expfile}: No sequence file for sequencing vector $template[5] | tee -a "$SQUIRREL_LOG" + goto skip + endif + if ( ! -e $SQUIRREL/seqs/$scfile[2] ) then + echo Abandoned: ${expfile}: Sequencing vector file does not exist | tee -a "$SQUIRREL_LOG" + goto skip + endif + + # information on cloning vector sequence files + set cfile = `$GET_LOOK_UP $clone[2] $TABLES_DIR/vector-seqfiles.lookup` + if ( $#cfile == 0 ) then + echo Abandoned: ${expfile}: No sequence file for cloning vector $clone[2] | tee -a "$SQUIRREL_LOG" + goto skip + endif + if ( ! -e $SQUIRREL/seqs/$cfile[2] ) then + echo Abandoned: ${expfile}: Cloning vector file does not exist | tee -a "$SQUIRREL_LOG" + goto skip + endif + + # include info derived from lookups + echo "TN $expname:r" >> $expfile + echo "SV $template[5]" >> $expfile + echo "SF $SQUIRREL/seqs/$scfile[2]" >> $expfile + echo "SI $template[3]" >> $expfile + echo "SC $subclone[2]" >> $expfile + echo "FM $template[4]" >> $expfile + + # assume Universal primer U1 + #echo PN U1 >> $expfile + + # determine whether forwards or reverse read + if ( $expname =~ *.[rR]* ) then + # these experiments are reverse reads + echo "DR -" >> $expfile + echo "SP $subclone[4]" >> $expfile + else + # otherwise assume forward read + echo "DR +" >> $expfile + echo "SP $subclone[3]" >> $expfile + endif + + # determine strands + if ( $expname =~ *.[rRfF]* ) then + # these experiments are double stranded + echo "ST 2" >> $expfile + else + # otherwise assume single stranded + echo "ST 1" >> $expfile + endif + + # include cloning vector information + echo "CN $template[2]" >> $expfile + echo "CV $clone[2]" >> $expfile + echo "CF $SQUIRREL/seqs/$cfile[2]" >> $expfile + + + # determine lists of screens + $GET_LOOK_UP $template[2] $TABLES_DIR/screens.lookup | awk '{$1="";print "SS " substr($0,2);}' >> $expfile + + + # retrieve the sequence + set temp_file = $expfile.temp.$$ + $GET_MACH_SEQ -SCF "$tracefile" -output $temp_file + if ( $status ) then + echo Discarded: ${expfile}: Error reading sequence from trace | tee -a "$SQUIRREL_LOG" + /bin/rm -f $temp_file + goto skip + endif + + # include sequence in experiment file + cat $temp_file >> $expfile + /bin/rm -f $temp_file + + # Determine poor quality parts + awk -f $GET_CLIP_SEQ $expfile >> $expfile + + # update file of file names + echo $expfile >> $2 + +# skip to here on error +skip: + +end + + +exit 0 + + +abort: +# +# interruption - not successful completion +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/excise-vector.csh b/src/squirrel-1.4/scripts/excise-vector.csh new file mode 100644 index 0000000..3f84d43 --- /dev/null +++ b/src/squirrel-1.4/scripts/excise-vector.csh @@ -0,0 +1,135 @@ +#! /bin/csh -f +# +# excise_vector +# Removes sequencing and cloning vector from sequences +# +# Usage: +# excise-vector in_fofn out_fofn +# +# 5-June-1992 +# vepe now writes fails to a fail file +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +# +# Check usage +# +if ( $#argv != 2 ) then + echo "Usage: excise-vector in_fofn out_fofn" | tee -a "$SQUIRREL_LOG" + exit 2 +endif +if ( ! -f $1 ) then + echo excise-vector: file of file names $1 does not exist. | tee -a "$SQUIRREL_LOG" + exit 1 +endif +if ( -e $2 ) then + echo excise-vector: file $2 exists. | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +# +# Where the wild things are +# +set EXCISE_VECTOR = $SQUIRREL/bin/vepe +set VEPE_FAILS = $SQUIRREL/scripts/vepe-fails.csh +# +# Temporary files +# +set temp_fofn = _vep_temp_fofn.$$ +set fails1 = _vep_fails1.$$ +set fails2 = _vep_fails2.$$ +/bin/rm -f ${temp_fofn} ${fails1} ${fails2} + +# +# Vep options, responses +# +set DEFAULT = "" + +#************************************************************ +# First pass - excise sequencing vector +#************************************************************ +echo "" | tee -a "$SQUIRREL_LOG" +echo "Excising sequencing vector" | tee -a "$SQUIRREL_LOG" + +${EXCISE_VECTOR} << EOF +1 +$1 +${temp_fofn} +${fails1} +${DEFAULT} +${DEFAULT} +${DEFAULT} +EOF +if ( $status ) then + echo "excise_vector: Abnormal termination" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +# +# Report failures +# +$VEPE_FAILS $fails1 1 +if ( $status ) then + echo "excise_vector: Abnormal termination" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + + + + + + + + + + + + + +#************************************************************ +# Second pass - excise cloning vector +#************************************************************ +echo "" | tee -a "$SQUIRREL_LOG" +echo "Excising cloning vector" | tee -a "$SQUIRREL_LOG" + +${EXCISE_VECTOR} << EOF +2 +${temp_fofn} +$2 +${fails2} +${DEFAULT} +${DEFAULT} +${DEFAULT} +EOF +if ( $status ) then + echo "excise_vector: Abnormal termination" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +# +# Report failures +# +$VEPE_FAILS $fails2 2 +if ( $status ) then + echo "excise_vector: Abnormal termination" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + + +# +# Tidy up +# +/bin/rm -f ${temp_fofn} +/bin/rm -f ${fails1} ${fails2} + +exit 0 + + +abort: +# +# interruption not normal exit +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/exp2dap.awk b/src/squirrel-1.4/scripts/exp2dap.awk new file mode 100644 index 0000000..6f0a460 --- /dev/null +++ b/src/squirrel-1.4/scripts/exp2dap.awk @@ -0,0 +1,157 @@ +# +# Convert experiment file format to a format compatible with dap +# +# It's pretty clutzy and nasty as information is lost in the conversion +# process. By it'll do until a version of "gap" is written that accepts +# files in the nice format. +# +# Written by Simon Dear, 10 February 1992 +# +# Changes: +# 3-Nov-92 Added code for creation of initial tags +# + +BEGIN { + # set createTags to "YES" if you want them + createTags = "YES"; +} + +$1 == "SQ" { + # Gather sequence + seq = ""; + getline; + while ( $0 != "//") { + # sequence may be broken up by white space + for (frag=1;frag<=NF;frag++) seq = seq $frag; + getline; + } + seqlen = length(seq); +} + +{ + # Record entry lines + type = $1; + $1 = ""; + entry[type] = substr($0,2); +} + + +END { + # Determine left and right end cuts + # Take all left and right ends of excluded sequences + n = 0; + if ( entry["CS"] != "" ) { + # special variables because we will need them later + CS = entry["CS"]; + CSl = substr(CS,1,index(CS,".")-1)+0; + CSr = substr(CS,index(CS,".")+2)+0; + n++; pos[n] = CSl; type[n] = +1; + n++; pos[n] = CSr; type[n] = -1; + } + if ( entry["SR"] != "" ) { + l = entry["SR"]+0; + r = seqlen+1; + n++; pos[n] = l; type[n] = +1; + n++; pos[n] = r; type[n] = -1; + } + if ( entry["QL"] != "" ) { + l = 0; + r = entry["QL"]+0; + n++; pos[n] = l; type[n] = +1; + n++; pos[n] = r; type[n] = -1; + } + if ( entry["QR"] != "" ) { + l = entry["QR"]+0; + r = seqlen+1; + n++; pos[n] = l; type[n] = +1; + n++; pos[n] = r; type[n] = -1; + } + if ( entry["SL"] != "" ) { + l = 0; + r = entry["SL"]+0.0; + n++; pos[n] = l; type[n] = +1; + n++; pos[n] = r; type[n] = -1; + } + + # Sort them + for (i = 2; i <= n; i++ ) { + for (j=i; j>1 && pos[j-1] > pos[j]; j--) { + #swap pos[j-1] and pos[j]; + t=pos[j-1]; pos[j-1]=pos[j]; pos[j]=t; + t=type[j-1]; type[j-1]=type[j]; type[j]=t; + } + } + + # Set defaults + pos[0] = 0; pos[n+1] = seqlen+1; + + # Deduce region not marked + # Select longest unmarked region of sequence + inn = 0; + max_width = 0; + for (i = 1; i <= n+1; i++ ) { + if (inn == 0) { + if (max_width < pos[i]-pos[i-1] ) { + l_cut = pos[i-1]; + r_cut = pos[i]; + max_width = pos[i]-pos[i-1]; + } + + } + inn += type[i]; + } + + # Write dap file header + ulen = r_cut - l_cut - 1; + printf(";%6d%6d%6d%-4s%-18s\n",seqlen,l_cut,ulen,entry["LT"],entry["LN"]); + + # Write left cutoffs (if any) + if(l_cut>0) { + str = substr(seq,1,l_cut); + strlen = l_cut; + bits = 48; + for (i = 1; i <= strlen; i+=bits) { + printf (";<%s\n",substr(str,i,bits)); + } + } + + # Write right cutoffs (if any) + if(r_cut<=seqlen) { + str = substr(seq,r_cut); + strlen = length(str); + bits = 48; + for (i = 1; i <= strlen; i+=bits) { + printf (";>%s\n",substr(str,i,bits)); + } + } + + if (createTags == "YES") { + # Write initial tags here + # IGNS + if (entry["SR"]+0 <= seqlen && ulen > 5) { + printf (";;IGNS %6d %6d %s\n",ulen-4,5,entry["SV"]); + } + # IGNC + if (entry["CS"] != "" && ulen > 5) { + # determine which end to put tag + if (CSr <= l_cut) { + # at the left + printf (";;IGNC %6d %6d %s\n",1,5,entry["CV"]); + } else { + # at the right + printf (";;IGNC %6d %6d %s\n",ulen-4,5,entry["CV"]); + } + } + } + + # Write remaining sequence (if any!) + if (l_cut+1 != r_cut) { + str = substr(seq,l_cut+1,r_cut-l_cut-1); + strlen = length(str); + bits = 50; + for (i = 1; i <= strlen; i+=bits) { + printf ("%s\n",substr(str,i,bits)); + } + } +} + diff --git a/src/squirrel-1.4/scripts/exp2fasta.awk b/src/squirrel-1.4/scripts/exp2fasta.awk new file mode 100644 index 0000000..1a5009d --- /dev/null +++ b/src/squirrel-1.4/scripts/exp2fasta.awk @@ -0,0 +1,129 @@ +# +# Convert experiment file format to fasta format +# +# It's pretty clutzy and nasty as information is lost in the conversion +# process. By it'll do until a version of "gap" is written that accepts +# files in the nice format. +# +# Written by Simon Dear, 10 February 1992 +# +# Changes: +# 3-Nov-92 Added code for creation of initial tags +# + +BEGIN { + # set createTags to "YES" if you want them + createTags = "YES"; +} + +$1 == "SQ" { + # Gather sequence + seq = ""; + getline; + while ( $0 != "//") { + # sequence may be broken up by white space + for (frag=1;frag<=NF;frag++) seq = seq $frag; + getline; + } + seqlen = length(seq); +} + +{ + # Record entry lines + type = $1; + $1 = ""; + entry[type] = substr($0,2); +} + + +END { + # Determine left and right end cuts + # Take all left and right ends of excluded sequences + n = 0; + if ( entry["CS"] != "" ) { + # special variables because we will need them later + CS = entry["CS"]; + CSl = substr(CS,1,index(CS,".")-1)+0; + CSr = substr(CS,index(CS,".")+2)+0; + n++; pos[n] = CSl; type[n] = +1; + n++; pos[n] = CSr; type[n] = -1; + } + if ( entry["SR"] != "" ) { + l = entry["SR"]+0; + r = seqlen+1; + n++; pos[n] = l; type[n] = +1; + n++; pos[n] = r; type[n] = -1; + } + if ( entry["QL"] != "" ) { + l = 0; + r = entry["QL"]+0; + n++; pos[n] = l; type[n] = +1; + n++; pos[n] = r; type[n] = -1; + } + if ( entry["QR"] != "" ) { + l = entry["QR"]+0; + r = seqlen+1; + n++; pos[n] = l; type[n] = +1; + n++; pos[n] = r; type[n] = -1; + } + if ( entry["SL"] != "" ) { + l = 0; + r = entry["SL"]+0.0; + n++; pos[n] = l; type[n] = +1; + n++; pos[n] = r; type[n] = -1; + } + + # Sort them + for (i = 2; i <= n; i++ ) { + for (j=i; j>1 && pos[j-1] > pos[j]; j--) { + #swap pos[j-1] and pos[j]; + t=pos[j-1]; pos[j-1]=pos[j]; pos[j]=t; + t=type[j-1]; type[j-1]=type[j]; type[j]=t; + } + } + + # Set defaults + pos[0] = 0; pos[n+1] = seqlen+1; + + # Deduce region not marked + # Select longest unmarked region of sequence + inn = 0; + max_width = 0; + for (i = 1; i <= n+1; i++ ) { + if (inn == 0) { + if (max_width < pos[i]-pos[i-1] ) { + l_cut = pos[i-1]; + r_cut = pos[i]; + max_width = pos[i]-pos[i-1]; + } + + } + inn += type[i]; + } + + # Write dap file header + ulen = r_cut - l_cut - 1; + printf(">%s\n",entry["EN"]); + + + # Write remaining sequence (if any!) + if (l_cut+1 != r_cut) { + str = substr(seq,l_cut+1,r_cut-l_cut-1); + strlen = length(str); + # convert all '-'s to Ns + str2 = "" + for (i=1; i<=strlen; i++) { + c = substr(str,i,1); + if (c=="-") + str2 = str2 "N"; + else + str2 = str2 c; + } + str = str2; + bits = 50; + for (i = 1; i <= strlen; i+=bits) { + printf ("%s\n",substr(str,i,bits)); + } + } +} + diff --git a/src/squirrel-1.4/scripts/log-to-summary.awk b/src/squirrel-1.4/scripts/log-to-summary.awk new file mode 100644 index 0000000..21054c4 --- /dev/null +++ b/src/squirrel-1.4/scripts/log-to-summary.awk @@ -0,0 +1,67 @@ +# +# log-to-summary.awk +# +# Generate a summary from a squirrel log file +# +# Possible usage: +# awk -f log-to-summary.awk logfile > summary +# +# Changes: +# 22-Oct-92 There are now defaults for readings lost in processing +# +BEGIN { + count = 0; +} + +/^\*\*/ { + # Format: ** expfile from trace + if (reverse[$2] == "") { + count++; + t = substr($0,length($2)+10); + sample[count] = $2; + trace[count] = t; + reverse[$2] = count; + # defaults + status[count] = "Abandoned"; + comment[count] = "lost in processing"; + + } +} + +/^Discarded:/ || /^Failed:/ || /^Abandoned:/ || /^OK:/ || /^Rejected:/ { + # Format: Failed: expfile: reason + # -or- Succeeded: expfile: comment + if (length($2) > 1) { + expfile = substr($2,1,length($2)-1); + NN = reverse[expfile]; + status[NN] = substr($1,1,length($1)-1); + comment[NN] = substr($0,length($1)+length($2)+3); + } +} + +#Miscellaneous lines +/^SQUIRREL/ || /^Processing/ || /^Project/ || /^Started at/ || /^Completed at/ || /^Aborted at/ || /^Pass/ || /^Resumed/ { + print "#", $0; +} + +END { + print "#"; + maxTrace = 0; maxSample = 0; maxSTATUS = 0; + for (i = 1 ; i <= count ; i++ ) { + if (sample[i] != "") { + if (maxTRACE < length(trace[i])) + maxTRACE = length(trace[i]); + if (maxSAMPLE < length(sample[i])) + maxSAMPLE = length(sample[i]); + if (maxSTATUS < length(status[i])) + maxSTATUS = length(status[i]); + } + } + fmt = "%-" maxTRACE+1 "s %-" maxSAMPLE "s %-" maxSTATUS "s %s\n"; + for (i = 1 ; i <= count ; i++ ) { + if (sample[i] != "") { + printf(fmt,trace[i] ":",sample[i],status[i],comment[i]); + } + } +} + diff --git a/src/squirrel-1.4/scripts/packhorse b/src/squirrel-1.4/scripts/packhorse new file mode 100644 index 0000000..e21329d --- /dev/null +++ b/src/squirrel-1.4/scripts/packhorse @@ -0,0 +1,84 @@ +#! /bin/csh -f +# +# File: packhorse +# Version: 1.1 +# +# Author: Simon Dear +# MRC Laboratory of Molecular Biology +# Hills Road +# Cambridge CB2 2QH +# United Kingdom +# +# Created: 13 October 1992 +# Updated: +# +# Description: +# C shell script to back ABI results files into one directory +# +# Usage: +# packhorse dir [dir...] directory +# + +# default number +set number = 0 + +if ($#argv < 2) then + echo 'Usage: packhorse dir [dir...] directory' + exit 2 +endif + +set packdir = "$argv[$#argv]" + +set logfile = "$packdir"/.PACK-LOG + +if (! -e "$packdir") then + mkdir "$packdir" +else if (! -d "$packdir") then + echo "$packdir" exists but is not a directory + exit 1 +else if ( -e "$packdir"/.rsrc ) then + echo "$packdir" is a Results folder...abandoned + exit 1 +else if ( ! -e "$logfile" ) then + echo "$packdir" is not a pack directory + exit 1 +endif + +touch "$logfile" + +set diri = 1 +set dirn = $#argv ; @ dirn -- +while ( $diri <= $dirn ) + set dir = "$argv[$diri]" + echo Packing "$dir" into "$packdir" | tee -a "$logfile" + # check number + set a = `find "$packdir" -name "Sample ${number}[0-9][0-9]" -print` + while ( $#a != 0 ) + @ number ++ + set a = `find "$packdir" -name "Sample ${number}[0-9][0-9]" -print` + end + # set files = `find "$dir" -prune -name "Sample [0-9][0-9]" -print` + set files = "$dir"/"Sample "[0-9][0-9]{,.[0-9]} + + set filei = 1 + while ( $filei <= $#files ) + set file = "$files[$filei]" + set newfile = `echo "$file" | sed 's/.*Sample /Sample '${number}'/'` + echo "$file -> $packdir/$newfile" | tee -a "$logfile" + mv "$file" "$packdir"/"$newfile" + @ filei ++ + end + + # remove directory, but only if no files left + # >100 disk blocks is arbitrary, but should trap any valid ABI files + set big_files = `find "$dir" -size +100 -print` + if ( $#big_files == 0 ) then + echo Removing directory "$dir" | tee -a "$logfile" + /bin/rm -r "$dir" + else + echo Not removing directory "$dir" - large files present | tee -a "$logfile" + endif + + @ diri ++ +end + diff --git a/src/squirrel-1.4/scripts/parse-session-file.awk b/src/squirrel-1.4/scripts/parse-session-file.awk new file mode 100644 index 0000000..e7ab09f --- /dev/null +++ b/src/squirrel-1.4/scripts/parse-session-file.awk @@ -0,0 +1,93 @@ +# +# Parse edited session file +# +# Input: +# +# (original session file) +# ##PASS-2## +# (edited session file) +# +# Output: +# (annotated edited session file) +# OUT> passed line... +# +# + +BEGIN { + FS = ":"; + pass = 1; + count = 0; + l = 0; +} + +/^##PASS-2##/ { + pass = 2; + next; +} + +pass == 2 { l++; printf("%3d %s\n",l,$0); } + +/^#/ { next; } + +pass == 1 { + pass1sample[$1] = 1; + next; +} + +pass == 2 { + + # must be two fields + if (NF != 2) { + print "ERROR: wrong number of fields >>", $0; + next; + } + + # must be non null + if (length($1)==0) { + print "ERROR: sample missing >>",$0; + next; + } + if (length($2)==0) { + print "ERROR: experiment missing >>",$0; + next; + } + + # $1 should be in original file + if (pass1sample[$1] != 1) { + print "ERROR: sample", $1, "was not in original file" + next; + } + + # $1 should not be duplicated + sample[$1]++; + if (sample[$1] > 1) { + print "ERROR: sample", $1, "appears more than once in edited file" + line[sample2line[$1]] = ""; + next; + } + + # $2 should look ok + for(i=1;i 1) { + print "ERROR: experiment", $2, "appears more than once in edited file" + line[experiment2line[$2]] = ""; + next; + } + + line[count] = $0; + sample2line[$1] = count; + experiment2line[$2] = count; + count ++; +} + +END { + for (i=0; i",line[i]; +} diff --git a/src/squirrel-1.4/scripts/quality-check-loose.awk b/src/squirrel-1.4/scripts/quality-check-loose.awk new file mode 100644 index 0000000..e425412 --- /dev/null +++ b/src/squirrel-1.4/scripts/quality-check-loose.awk @@ -0,0 +1,74 @@ +# +# Determine if the experiment file should be rejected because of +# poor quality. Ideally this would be done by looking at trace +# quality, but this will have to do in the meantime +# +# Method: +# Determine the extents of the good data, by looking at the +# experiment file entries. Default is to use LL..RR +# Reject if the good data is less that MINLEN +# Reject if the good data has greater than CRAP (%) ambiguity content +# +# Outputs: +# 0 - passes quality check +# 1 - fails length constraint +# 2 - fails ambiguity content constraint +# +# Written by Simon Dear, 27 March 1992 +# +# loose version +# + +BEGIN { + LL = 50; + RR = 350; + MINLEN = 50; + CRAP = 10.0; +} + +$1 == "SQ" { + # Gather sequence + seq = ""; + getline; + while ( $0 != "//") { + # sequence may be broken up by white space + for (frag=1;frag<=NF;frag++) seq = seq $frag; + getline; + } + seqlen = length(seq); +} + +{ + # Record entry lines + type = $1; + $1 = ""; + entry[type] = substr($0,2); +} + + +END { + if ( entry["QL"] != "" ) LL = entry["QL"] + 0; + if ( entry["QR"] != "" ) RR = entry["QR"] + 0; + if ( LL > seqlen ) LL = seqlen; + if ( RR > seqlen ) RR = seqlen; + good_len = RR - LL + 1; + + # Check length constraint + if ( good_len < MINLEN ) { + print 1; + } else { + # Check ambiguity content restraint + + count = 0; + for (i = LL; i <= RR; i++) { + + if ( index("ACGTacgt",substr(seq,i,1)) == 0 ) count++; + + } + + if ( count * 100 / good_len > CRAP ) + print 2; + else + print 0; + } +} diff --git a/src/squirrel-1.4/scripts/quality-check.awk b/src/squirrel-1.4/scripts/quality-check.awk new file mode 100644 index 0000000..750fe63 --- /dev/null +++ b/src/squirrel-1.4/scripts/quality-check.awk @@ -0,0 +1,72 @@ +# +# Determine if the experiment file should be rejected because of +# poor quality. Ideally this would be done by looking at trace +# quality, but this will have to do in the meantime +# +# Method: +# Determine the extents of the good data, by looking at the +# experiment file entries. Default is to use LL..RR +# Reject if the good data is less that MINLEN +# Reject if the good data has greater than CRAP (%) ambiguity content +# +# Outputs: +# 0 - passes quality check +# 1 - fails length constraint +# 2 - fails ambiguity content constraint +# +# Written by Simon Dear, 27 March 1992 +# + +BEGIN { + LL = 50; + RR = 350; + MINLEN = 100; + CRAP = 4.0; +} + +$1 == "SQ" { + # Gather sequence + seq = ""; + getline; + while ( $0 != "//") { + # sequence may be broken up by white space + for (frag=1;frag<=NF;frag++) seq = seq $frag; + getline; + } + seqlen = length(seq); +} + +{ + # Record entry lines + type = $1; + $1 = ""; + entry[type] = substr($0,2); +} + + +END { + if ( entry["QL"] != "" ) LL = entry["QL"] + 0; + if ( entry["QR"] != "" ) RR = entry["QR"] + 0; + if ( LL > seqlen ) LL = seqlen; + if ( RR > seqlen ) RR = seqlen; + good_len = RR - LL + 1; + + # Check length constraint + if ( good_len < MINLEN ) { + print 1; + } else { + # Check ambiguity content restraint + + count = 0; + for (i = LL; i <= RR; i++) { + + if ( index("ACGTacgt",substr(seq,i,1)) == 0 ) count++; + + } + + if ( count * 100 / good_len > CRAP ) + print 2; + else + print 0; + } +} diff --git a/src/squirrel-1.4/scripts/screen-against-vector.csh b/src/squirrel-1.4/scripts/screen-against-vector.csh new file mode 100644 index 0000000..5d502c1 --- /dev/null +++ b/src/squirrel-1.4/scripts/screen-against-vector.csh @@ -0,0 +1,155 @@ +#! /bin/csh -f +# +# screen-against-vector +# +# Screen files against sequences +# +# Usage: +# screen-against-vector in_fofn out_fofn +# +# NOTE: +# This script: +# 1) determines a list of files to screen against +# 2) extracts the clipped sequences from the experiment files +# 3) screens each sequence against the files in (1) +# +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +# +# Check usage +# +if ( $#argv != 2 ) then + echo Usage: screen-against-vector in_fofn out_fofn | tee -a "$SQUIRREL_LOG" + exit 2 +endif + +if ( ! -f $1 ) then + echo screen-against-vector: file of file names $1 does not exist | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +if ( -e $2 ) then + echo screen-against-vector: output file of file names $2 already exists | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +# +# Scripts and programs +# +set EXPAND = $SQUIRREL/scripts/staden-env.csh +set CONVERT = $SQUIRREL/scripts/exp2dap.awk + +# +# Temporary files +# +set in = _in_$$ +set out = _out_$$ +set hits = _hits_$$ +set misses = _misses_$$ +set tmpdir = _tmpdir_$$ + +# +# Determine all sequences to screen against +# +set expfiles = `cat $1` +set f = `awk '/^SS/{$1="";print substr($0,2);}' $expfiles | sort -u` + +# +# Set up for dap/screen against vector +# +sort $1 >! $in + +if ( $#f != 0 ) then + if ( -d $tmpdir ) /bin/rm -fr $tmpdir + mkdir $tmpdir + foreach file ( $expfiles ) + awk -f $CONVERT $file > $tmpdir/$file + end +endif + +set VECTOR_SCORE = 25 +foreach vector ( $f ) + + echo Screening against $vector | tee -a "$SQUIRREL_LOG" + + if (`wc -l $in | awk '{print $1;}'` == 0) goto skip + + # + set xvec = $SQUIRREL/seqs/$vector + if (! -f $xvec) then + echo vector file $xvec does not exist...skipping | tee -a "$SQUIRREL_LOG" + goto skip + endif + # this is a hack to make bap happy + set xvec = SQUIRREL/seqs/$vector + + # determine files to screen against + grep -l $vector `cat $in` >! $hits + comm -23 $in $hits >! $misses + + /bin/rm -f $out +pushd $tmpdir > /dev/null +bap << EOF +3 +! + +18 +y +../$hits +../$out +$xvec +$VECTOR_SCORE + +! +EOF + + + # + # Check exit status + # + if ( $status ) then + echo screen-against-vector: Error running bap/screen against vector | tee -a "$SQUIRREL_LOG" + exit 1 + endif + + popd > /dev/null + echo "" | tee -a "$SQUIRREL_LOG" + + # + # Print out Failed this pass + # + tr -d ' ' < $out >! $in ; /bin/mv -f $in $out + foreach file ( `comm -23 $hits $out` ) + echo Failed: ${file}: Matches screening sequence $vector | tee -a "$SQUIRREL_LOG" + echo "PS Matches screening sequence $vector" >> ${file} + end + + # + # Determine sequences passed to next round + # + sort $misses $out >! $in + +skip: +end + + +# transfer successful passes to output file +/bin/cp $in $2 + +# +# Remove temporary files +# +/bin/rm -f $in $out $hits $misses +/bin/rm -fr $tmpdir + +exit 0 + +abort: +# +# interruption not normal exit +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/screen-against-vector.csh.old b/src/squirrel-1.4/scripts/screen-against-vector.csh.old new file mode 100644 index 0000000..ce17499 --- /dev/null +++ b/src/squirrel-1.4/scripts/screen-against-vector.csh.old @@ -0,0 +1,77 @@ +#! /bin/csh -f +# +# screen-against-vector +# +# Screen files against vector sequence +# +# Usage: +# screen-against-vector vector_file in_fofn out_fofn +# +# NOTE: +# This script uses xdap, which as yet cannot handle the experiment +# file format. Eventually, vepe will be modified to do the same task. +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +# +# Check usage +# +if ( $#argv != 3 ) then + echo Usage: screen-against-vector vector_file in_fofn out_fofn + exit 2 +endif + +if ( ! -f $1 ) then + echo screen-against-vector: vector file $1 does not exist + exit 1 +endif + +if ( ! -f $2 ) then + echo screen-against-vector: file of file names $2 does not exist + exit 1 +endif + +if ( -e $3 ) then + echo screen-against-vector: output file of file names $3 already exists + exit 1 +endif + + +# +# Set up for dap/screen against vector +# +set VECTOR_SCORE = 20 +setenv VECTOR_FILE $1 + +dap << EOF +3 +! + +18 +y +$2 +$3 +VECTOR_FILE +$VECTOR_SCORE + +! +EOF + +# +# Check exit status +# +if ( $status ) then + echo screen-against-vector: Error running dap/screen against vector + exit 1 +endif + +exit 0 + +abort: +# +# interruption not normal exit +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/squirrel b/src/squirrel-1.4/scripts/squirrel new file mode 100644 index 0000000..959aba5 --- /dev/null +++ b/src/squirrel-1.4/scripts/squirrel @@ -0,0 +1,73 @@ +#! /bin/csh +# +# squirrel +# A small brown furry animal +# +# Usage +# squirrel machine_letter date +# + +# +# Define the project +# +set PROJECT = TESTDB + +# +# Define the database to use if different from default +# +#setenv SQUIRREL_SUB H4 + + +onintr abort + +# Usage +if ( $#argv != 2 ) then + echo "Usage: squirrel machine_letter date" + exit 2 +endif + +set transfer_directory = "$HOME/Mac-files-$USER/Results $1 $2" +if ( ! -d "$transfer_directory" ) then + echo "squirrel: Bad folder machine or date" + exit 1 +endif + +# +# Where the brown and furry things are +# +if (! $?SQUIRREL) then + echo "Error - environment variable SQUIRREL not defined" + #setenv SQUIRREL /nfs/al/cb0/wormpub/BioSW/squirrel + exit 1 +endif +setenv EDITOR emacs + +# +# Programs and scripts +# +# to assemble automatically +# set PROCESS = $SQUIRREL/scripts/do-it.csh +# to process without assembly +# set PROCESS = $SQUIRREL/scripts/do-it-no-assembly.csh +set PROCESS = $SQUIRREL/scripts/do-it.csh + +# +# The real work starts here +# +$PROCESS "$transfer_directory" $PROJECT +if ( $status ) then + echo "squirrel: Error occurred during processing." + goto abort +endif + +# +# Successful completion +# +exit 0 + +abort: +# +# Interruption not normal termination +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/tidy-files.csh b/src/squirrel-1.4/scripts/tidy-files.csh new file mode 100644 index 0000000..df7f934 --- /dev/null +++ b/src/squirrel-1.4/scripts/tidy-files.csh @@ -0,0 +1,65 @@ +#! /bin/csh -f +# +# tidy-files +# Remove processed (not abandoned) files from transfer directory +# +# Usage: +# tidy-files session-file +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +# +# Check usage +# +if ( $#argv != 1 ) then + echo "Usage: tidy-files session-file | tee -a "$SQUIRREL_LOG" + exit 2 +endif + +if ( ! -e $1 ) then + echo tidy-files: Session log file does not exist | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +# +# Programs and scripts and things +# + +# +# Temporary files +# +set includes = _incl.$$ +set commands = _comm.$$ +/bin/rm -f $includes $commands + +echo "" | tee -a "$SQUIRREL_LOG" +echo Tidying up transfer directory | tee -a "$SQUIRREL_LOG" + +# +# Remove unneeded files in directory +# +awk 'BEGIN {FS=":";} /^#/ {next;} /Abandon/ {next;} {print $1;}' $1 > $includes +sed 's/^/\/bin\/rm -f "/;s/$/"/' $includes > $commands +source $commands + +/bin/rm -f $1 +/bin/rm _* + + +# +# Remove temporary files +# +/bin/rm -f $includes $commands + + + +exit 0 + +abort: +# +# interruption not normal exit +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/transfer-files.csh b/src/squirrel-1.4/scripts/transfer-files.csh new file mode 100644 index 0000000..d8a5930 --- /dev/null +++ b/src/squirrel-1.4/scripts/transfer-files.csh @@ -0,0 +1,141 @@ +#! /bin/csh -f +# +# transfer-files +# Copies files specified by a file of file names from the current +# directory to the project_directory, making file format changes +# where necessary. +# +# When running with dap experiment files are converted to xdap files. +# +# Usage: +# transfer-files project-directory in_fofn out_fofn +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +# +# Check usage +# +if ( $#argv != 3 ) then + echo "Usage: transfer-files project-directory in_fofn out_fofn" | tee -a "$SQUIRREL_LOG" + exit 2 +endif + + +if ( ! -d $1 ) then + echo "transfer-files: Project directory $1 does not exist" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +if ( ! -e $2 ) then + echo "transfer-files: File of file names $2 does not exist" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +if ( -e $1/$3 ) then + echo "transfer-files: File of file names $1/$3 already exists" | tee -a "$SQUIRREL_LOG" + exit 1 +endif + +set CONVERT_EXP_FILE = $SQUIRREL/scripts/exp2dap.awk + +# +# Save typing if need to change +# +set FAILURES = $1/Failures +set EXPFILES = $1/Expfiles + +# Repository for experiment files and failures +if (! -e ${EXPFILES}) mkdir ${EXPFILES} +if (! -e ${FAILURES}) mkdir ${FAILURES} + +echo "" | tee -a "$SQUIRREL_LOG" +echo "Transferring files from $cwd to $1" | tee -a "$SQUIRREL_LOG" +touch $1/$3 +foreach file ( `cat $2` ) + + # Check file exists in transfer directory + if (! -e $file ) then + # check it is processed + if ( ! -e ${EXPFILES}/$file ) then + echo Abandoned: ${file}: Problems transferring | tee -a "$SQUIRREL_LOG" + goto skip + endif + + echo transfer-files: warning - $file already transferred + # set PS = `awk '/^PS/ {$1="";line = substr($0,2);} END {print line;}' ${EXPFILES}/$file` + # goto recover + goto skip + endif + + # Ensure files don't already exist + /bin/rm -f $1/$file ${EXPFILES}/$file ${FAILURES}/$file + + # Determine directory for sequence files and trace files + set PS = `awk '/^PS/ {$1="";line = substr($0,2);} END {print line;}' $file` + + if ( "$PS" == "" ) then + set dir = "$1" + else + if ( $PS[1] == "Matches" ) then + set dir = "$1" + else + set dir = "${FAILURES}" + endif + endif + + + # Convert experiment file + awk -f $CONVERT_EXP_FILE $file > $dir/$file + if ( $status ) then + echo Abandoned: ${file}: Converting experiment file + /bin/rm -f $dir/$file + goto skip + endif + + # Transfer Local Trace file + set LN = `awk '/^LN/ {$1="";line = substr($0,2);} END {print line;}' $file` + if ( "$LN" != "" ) then + /bin/cp $LN $dir + if ( $status ) then + echo Abandoned: ${file}: Transferring trace file + /bin/rm -f $dir/$LN + goto skip + endif + endif + + # Copy experiment file + # This should be the last operation before removal of files + /bin/cp $file ${EXPFILES} + if ( $status ) then + echo "transfer-files.csh: failed to copy experiment file" + endif + + # Get rid of files + /bin/rm -f $file $LN + +recover: + # If not marked for failure add to file of file names + if ( "$PS" == "" ) then + echo $file >> $1/$3 + else + if ( $PS[1] == "Matches" ) then + # have a match with a vector... add to screen file + set failf = "$1"/Screen.$PS[4] + if ( ! -e "$failf" ) touch "$failf" + echo $file >> "$failf" + endif + endif + +skip: +end + +exit 0 + +abort: +# +# interruption not normal exit +# + +exit 1 diff --git a/src/squirrel-1.4/scripts/trim-seq.awk b/src/squirrel-1.4/scripts/trim-seq.awk new file mode 100644 index 0000000..158d0f4 --- /dev/null +++ b/src/squirrel-1.4/scripts/trim-seq.awk @@ -0,0 +1,90 @@ +# +# Clip sequence on basis of sequence content - +# +# (xdap version) +# +# Rule: everything is good quality up to where NN uncertainties in +# MM window, in the base range LL..RR +# +# Outputs new experiment file with extra clip line to stdout +# +# St.Louis use NN=2, MM=5, LL=200, RR=sequence-length +# + +BEGIN { + # Up to where NN uncertainties in MM window starting at base SS + NN = 2; + MM = 5; + LL = 200; + RR = 400; +} + + +/;/ { rseq = rseq substr($0,3); next; } +/;/ { header = $0; next; } + { mseq = mseq $0; next; } + +END { + seq = lseq mseq rseq; + + seqlen = length(seq); + lcut = length(lseq); + + if (RR > seqlen) RR = seqlen; + rcut = lcut + length(mseq) + 1; + # rcut = RR+1; + if ( NN>0 ) { + score = 0; + for(i=LL-MM+1; i<=RR-MM+1; i++) { + if (i>=MM) { + if (index("ACGTacgt",substr(seq,i,1)) == 0) score-- + } + if (i+MM-1<=RR) { + if (index("ACGTacgt",substr(seq,i+MM-1,1)) == 0) score++; + } + if (score >= NN) { + rcut = i; + break; + } + } + } + + # Write dap file header + if (length(header)==0) { + printf(";%6d%6d%6d%-4s%-18s\n",seqlen,lcut,rcut-lcut-1,"PLN",""); + } else { + printf(";%6d%6d%6d%s\n",seqlen,lcut,rcut-lcut-1,substr(header,20)); + } + + # Write left cutoffs (if any) + if(lcut>0) { + str = substr(seq,1,lcut); + strlen = lcut; + bits = 48; + for (i = 1; i <= strlen; i+=bits) { + printf (";<%s\n",substr(str,i,bits)); + } + } + + # Write right cutoffs (if any) + if(rcut<=seqlen) { + str = substr(seq,rcut); + strlen = length(str); + bits = 48; + for (i = 1; i <= strlen; i+=bits) { + printf (";>%s\n",substr(str,i,bits)); + } + } + + # Write remaining sequence (if any!) + if (lcut+1 != rcut) { + str = substr(seq,lcut+1,rcut-lcut-1); + strlen = length(str); + bits = 50; + for (i = 1; i <= strlen; i+=bits) { + printf ("%s\n",substr(str,i,bits)); + } + } + +} diff --git a/src/squirrel-1.4/scripts/vepe-fails.csh b/src/squirrel-1.4/scripts/vepe-fails.csh new file mode 100644 index 0000000..90dfa95 --- /dev/null +++ b/src/squirrel-1.4/scripts/vepe-fails.csh @@ -0,0 +1,113 @@ +#! /bin/csh -f +# +# vepe-fails +# Parse a vepe failure file and report errors +# +# Usage: +# vepe-fails vepe_fail_file vepe_task +# +# where vepe_task is +# 1 Mark sequencing vector +# 2 Mark cloning vector +# + +onintr abort +if (! $?SQUIRREL_LOG) setenv SQUIRREL_LOG /dev/null + +# +# Check usage +# +if ( $#argv != 2 ) then + echo "Usage: vepe-fails vepe_fail_file vepe_task" | tee -a "$SQUIRREL_LOG" + exit 2 +endif +if ( ! -f $1 ) then + echo vepe-fails: failure file does not exist | tee -a "$SQUIRREL_LOG" + exit 1 +endif + + + +# +# Strip out failures and reasons +# +set expfiles = `awk '{print $1;}' $1` +set errcodes = `awk '{if (NF==2)print $2;else print -1;}' $1` + +while ( $#expfiles ) + + set expname = $expfiles[1] + set expfile = $expfiles[1] + set errcode = $errcodes[1] + + switch(${errcode}) + case 1: + set reason = "Couldn't open experiment file" + breaksw; + case 2: + set reason = "No reading" + breaksw; + case 3: + set reason = "Reading too short for vep" + breaksw; + case 4: + set reason = "No vector file name" + breaksw; + case 5: + set reason = "No cloning site" + breaksw; + case 6: + set reason = "No priming site" + breaksw; + case 7: + set reason = "Couldn't open vector file" + breaksw; + case 8: + set reason = "Error writing to experiment file" + breaksw; + case 9: + switch ( $2 ) + case 1: + set reason = "Completely sequencing vector" + breaksw + case 2: + set reason = "Completely cloning vector" + breaksw + default: + set reason = "Completely vector" + breaksw + endsw + breaksw; + default: + set reason = "Reason unknown" #Unknown + breaksw; + endsw + + if ( ${errcode} == 3 || ${errcode} == 9 ) then + # vep failures + echo Failed: ${expname}: $reason | tee -a "$SQUIRREL_LOG" + if (-e ${expfile}) echo "PS $reason" >> ${expfile} + else + # vep abandons + echo Abandoned: ${expname}: Vep failed : $reason | tee -a "$SQUIRREL_LOG" + endif + + + # Cycle + shift expfiles + shift errcodes +end + + + + + +exit 0 + + +abort: +# +# interruption not normal exit +# + +exit 1 diff --git a/src/squirrel-1.4/seqs/Alu.seq b/src/squirrel-1.4/seqs/Alu.seq new file mode 100644 index 0000000..718b19a --- /dev/null +++ b/src/squirrel-1.4/seqs/Alu.seq @@ -0,0 +1,8 @@ +;Alu consensus ex PROTOTYPIC SEQENCES FOR HUMAN REPETITIVE DNA + aatggcagaatgacacttcncaaaagaagacatttatgggccgggcgctttggctcacgc + cctgtaatcccagcactttgggaggccgaggcgggcggatcacgaggtcaggagatcgag + accatcccggctaaaacggtgaaaccccgtctctactaaaactacaaaaaatagccgggc + gtagtgcgggcgcctgtagtcctagctacttgggaggctgaggcaggagaatggcgtgaa + cccgggaggcggagcttgcagtgagccgagatcccgccactgcactccagcctgggtgac + agagcgagactccatctcaaaaaaaaaaaaaaaaaaaaaaaaaaaaagaagacatttatg + cagccaaaaaacacatgaaaaatgctcatcatcagagaaggcaatcaaaccacatg diff --git a/src/squirrel-1.4/seqs/README b/src/squirrel-1.4/seqs/README new file mode 100644 index 0000000..458277c --- /dev/null +++ b/src/squirrel-1.4/seqs/README @@ -0,0 +1,4 @@ + Dir: $SQUIRREL/seqs/ +Date: Fri May 07 12:10:51 BST 1993 + +In this directory should be placed any sequences squirrel requires. diff --git a/src/squirrel-1.4/seqs/blue.vep b/src/squirrel-1.4/seqs/blue.vep new file mode 100644 index 0000000..1a48a74 --- /dev/null +++ b/src/squirrel-1.4/seqs/blue.vep @@ -0,0 +1,55 @@ +GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATA +ATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGG +AACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCA +TGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT +ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT +TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATG +CTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC +AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT +GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACG +CCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG +GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGT +AAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA +ACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG +CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT +GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAA +TGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT +TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC +ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTG +GAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT +GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC +TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA +AGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGAT +TTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGA +TAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGT +CAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTG +CGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGT +TTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCT +TCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTA +GGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCT +AATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCG +GGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGA +ACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGA +ACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAG +GGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAG +CGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGT +CGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAG +GGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTC +CTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCC +TGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTC +GCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAA +GAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTA +ATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCA +ACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACAC +TTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATT +TCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCGGAATTAACC +CTCACTAAAGGGAACAAAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGA +TCCCCGGGTACCGAGCTCGAATTCGCCCTATAGTGAGTCGTATTACAATT +CACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACC +CAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAG +CGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATG +GCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATT +TCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATA +GTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGG +CTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGG +AGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGA diff --git a/src/squirrel-1.4/seqs/lambda.seq b/src/squirrel-1.4/seqs/lambda.seq new file mode 100644 index 0000000..c49bf21 --- /dev/null +++ b/src/squirrel-1.4/seqs/lambda.seq @@ -0,0 +1,810 @@ +<---LAMBDA.001-----> +GGGCGGCGACCTCGCGGGTTTTCGCTATTTATGAAAATTTTCCGGTTTAAGGCGTTTCCG +TTCTTCTTCGTCATAACTTAATGTTTTTATTTAAAATACCCTCTGAAAAGAAAGGAAACG +ACAGGTGCTGAAAGCGAGGCTTTTTGGCCTCTGTCGTTTCCTTTCTCTGTTTTTGTCCGT +GGAATGAACAATGGAAGTCAACAAAAAGCAGCTGGCTGACATTTTCGGTGCGAGTATCCG +TACCATTCAGAACTGGCAGGAACAGGGAATGCCCGTTCTGCGAGGCGGTGGCAAGGGTAA +TGAGGTGCTTTATGACTCTGCCGCCGTCATAAAATGGTATGCCGAAAGGGATGCTGAAAT +TGAGAACGAAAAGCTGCGCCGGGAGGTTGAAGAACTGCGGCAGGCCAGCGAGGCAGATCT +CCAGCCAGGAACTATTGAGTACGAACGCCATCGACTTACGCGTGCGCAGGCCGACGCACA +GGAACTGAAGAATGCCAGAGACTCCGCTGAAGTGGTGGAAACCGCATTCTGTACTTTCGT +GCTGTCGCGGATCGCAGGTGAAATTGCCAGTATTCTCGACGGGCTCCCCCTGTCGGTGCA +GCGGCGTTTTCCGGAACTGGAAAACCGACATGTTGATTTCCTGAAACGGGATATCATCAA +AGCCATGAACAAAGCAGCCGCGCTGGATGAACTGATACCGGGGTTGCTGAGTGAATATAT +CGAACAGTCAGGTTAACAGGCTGCGGCATTTTGTCCGCGCCGGGCTTCGCTCACTGTTCA +GGCCGGAGCCACAGACCGCCGTTGAATGGGCGGATGCTAATTACTATCTCCCGAAAGAAT +CCGCATACCAGGAAGGGCGCTGGGAAACACTGCCCTTTCAGCGGGCCATCATGAATGCGA +TGGGCAGCGACTACATCCGTGAGGTGAATGTGGTGAAGTCTGCCCGTGTCGGTTATTCCA +AAATGCTGCTGGGTGTTTATGCCTACTTTATAGAGCATAAGCAGCGCAACACCCTTATCT +GGTTGCCGACGGATGGTGATGCCGAGAACTTTATGAAAACCCACGTTGAGCCGACTATTC +GTGATATTCCGTCGCTGCTGGCGCTGGCCCCGTGGTATGGCAAAAAGCACCGGGATAACA +CGCTCACCATGAAGCGTTTCACTAATGGGCGTGGCTTCTGGTGCCTGGGCGGTAAAGCGG +CAAAAAACTACCGTGAAAAGTCGGTGGATGTGGCGGGTTATGATGAACTTGCTGCTTTTG +ATGATGATATTGAACAGGAAGGCTCTCCGACGTTCCTGGGTGACAAGCGTATTGAAGGCT +CGGTCTGGCCAAAGTCCATCCGTGGCTCCACGCCAAAAGTGAGAGGCACCTGTCAGATTG +AGCGTGCAGCCAGTGAATCCCCGCATTTTATGCGTTTTCATGTTGCCTGCCCGCATTGCG +GGGAGGAGCAGTATCTTAAATTTGGCGACAAAGAGACGCCGTTTGGCCTCAAATGGACGC +CGGATGACCCCTCCAGCGTGTTTTATCTCTGCGAGCATAATGCCTGCGTCATCCGCCAGC +AGGAGCTGGACTTTACTGATGCCCGTTATATCTGCGAAAAGACCGGGATCTGGACCCGTG +ATGGCATTCTCTGGTTTTCGTCATCCGGTGAAGAGATTGAGCCACCTGACAGTGTGACCT +TTCACATCTGGACAGCGTACAGCCCGTTCACCACCTGGGTGCAGATTGTCAAAGACTGGA +TGAAAACGAAAGGGGATACGGGAAAACGTAAAACCTTCGTAAACACCACGCTCGGTGAGA +CGTGGGAGGCGAAAATTGGCGAACGTCCGGATGCTGAAGTGATGGCAGAGCGGAAAGAGC +ATTATTCAGCGCCCGTTCCTGACCGTGTGGCTTACCTGACCGCCGGTATCGACTCCCAGC +TGGACCGCTACGAAATGCGCGTATGGGGATGGGGGCCGGGTGAGGAAAGCTGGCTGATTG +ACCGGCAGATTATTATGGGCCGCCACGACGATGAACAGACGCTGCTGCGTGTGGATGAGG +CCATCAATAAAACCTATACCCGCCGGAATGGTGCAGAAATGTCGATATCCCGTATCTGCT +GGGATACTGGCGGGATTGACCCGACCATTGTGTATGAACGCTCGAAAAAACATGGGCTGT +TCCGGGTGATCCCCATTAAAGGGGCATCCGTCTACGGAAAGCCGGTGGCCAGCATGCCAC +GTAAGCGAAACAAAAACGGGGTTTACCTTACCGAAATCGGTACGGATACCGCGAAAGAGC +AGATTTATAACCGCTTCACACTGACGCCGGAAGGGGATGAACCGCTTCCCGGTGCCGTTC +ACTTCCCGAATAACCCGGATATTTTTGATCTGACCGAAGCGCAGCAGCTGACTGCTGAAG +AGCAGGTCGAAAAATGGGTGGATGGCAGGAAAAAAATACTGTGGGACAGCAAAAAGCGAC +GCAATGAGGCACTCGACTGCTTCGTTTATGCGCTGGCGGCGCTGCGCATCAGTATTTCCC +GCTGGCAGCTGGATCTCAGTGCGCTGCTGGCGAGCCTGCAGGAAGAGGATGGTGCAGCAA +CCAACAAGAAAACACTGGCAGATTACGCCCGTGCCTTATCCGGAGAGGATGAATGACGCG +ACAGGAAGAACTTGCCGCTGCCCGTGCGGCACTGCATGACCTGATGACAGGTAAACGGGT +GGCAACAGTACAGAAAGACGGACGAAGGGTGGAGTTTACGGCCACTTCCGTGTCTGACCT +GAAAAAATATATTGCAGAGCTGGAAGTGCAGACCGGCATGACACAGCGACGCAGGGGACC +TGCAGGATTTTATGTATGAAAACGCCCACCATTCCCACCCTTCTGGGGCCGGACGGCATG +ACATCGCTGCGCGAATATGCCGGTTATCACGGCGGTGGCAGCGGATTTGGAGGGCAGTTG +CGGTCGTGGAACCCACCGAGTGAAAGTGTGGATGCAGCCCTGTTGCCCAACTTTACCCGT +GGCAATGCCCGCGCAGACGATCTGGTACGCAATAACGGCTATGCCGCCAACGCCATCCAG +CTGCATCAGGATCATATCGTCGGGTCTTTTTTCCGGCTCAGTCATCGCCCAAGCTGGCGC +TATCTGGGCATCGGGGAGGAAGAAGCCCGTGCCTTTTCCCGCGAGGTTGAAGCGGCATGG +AAAGAGTTTGCCGAGGATGACTGCTGCTGCATTGACGTTGAGCGAAAACGCACGTTTACC +ATGATGATTCGGGAAGGTGTGGCCATGCACGCCTTTAACGGTGAACTGTTCGTTCAGGCC +ACCTGGGATACCAGTTCGTCGCGGCTTTTCCGGACACAGTTCCGGATGGTCAGCCCGAAG +CGCATCAGCAACCCGAACAATACCGGCGACAGCCGGAACTGCCGTGCCGGTGTGCAGATT +AATGACAGCGGTGCGGCGCTGGGATATTACGTCAGCGAGGACGGGTATCCTGGCTGGATG +CCGCAGAAATGGACATGGATACCCCGTGAGTTACCCGGCGGGCGCGCCTCGTTCATTCAC +GTTTTTGAACCCGTGGAGGACGGGCAGACTCGCGGTGCAAATGTGTTTTACAGCGTGATG +GAGCAGATGAAGATGCTCGACACGCTGCAGAACACGCAGCTGCAGAGCGCCATTGTGAAG +GCGATGTATGCCGCCACCATTGAGAGTGAGCTGGATACGCAGTCAGCGATGGATTTTATT +CTGGGCGCGAACAGTCAGGAGCAGCGGGAAAGGCTGACCGGCTGGATTGGTGAAATTGCC +GCGTATTACGCCGCAGCGCCGGTCCGGCTGGGAGGCGCAAAAGTACCGCACCTGATGCCG +GGTGACTCACTGAACCTGCAGACGGCTCAGGATACGGATAACGGCTACTCCGTGTTTGAG +CAGTCACTGCTGCGGTATATCGCTGCCGGGCTGGGTGTCTCGTATGAGCAGCTTTCCCGG +AATTACGCCCAGATGAGCTACTCCACGGCACGGGCCAGTGCGAACGAGTCGTGGGCGTAC +TTTATGGGGCGGCGAAAATTCGTCGCATCCCGTCAGGCGAGCCAGATGTTTCTGTGCTGG +CTGGAAGAGGCCATCGTTCGCCGCGTGGTGACGTTACCTTCAAAAGCGCGCTTCAGTTTT +CAGGAAGCCCGCAGTGCCTGGGGGAACTGCGACTGGATAGGCTCCGGTCGTATGGCCATC +GATGGTCTGAAAGAAGTTCAGGAAGCGGTGATGCTGATAGAAGCCGGACTGAGTACCTAC +GAGAAAGAGTGCGCAAAACGCGGTGACGACTATCAGGAAATTTTTGCCCAGCAGGTCCGT +GAAACGATGGAGCGCCGTGCAGCCGGTCTTAAACCGCCCGCCTGGGCGGCTGCAGCATTT +GAATCCGGGCTGCGACAATCAACAGAGGAGGAGAAGAGTGACAGCAGAGCTGCGTAATCT +CCCGCATATTGCCAGCATGGCCTTTAATGAGCCGCTGATGCTTGAACCCGCCTATGCGCG +GGTTTTCTTTTGTGCGCTTGCAGGCCAGCTTGGGATCAGCAGCCTGACGGATGCGGTGTC +CGGCGACAGCCTGACTGCCCAGGAGGCACTCGCGACGCTGGCATTATCCGGTGATGATGA +CGGACCACGACAGGCCCGCAGTTATCAGGTCATGAACGGCATCGCCGTGCTGCCGGTGTC +CGGCACGCTGGTCAGCCGGACGCGGGCGCTGCAGCCGTACTCGGGGATGACCGGTTACAA +CGGCATTATCGCCCGTCTGCAACAGGCTGCCAGCGATCCGATGGTGGACGGCATTCTGCT +CGATATGGACACGCCCGGCGGGATGGTGGCGGGGGCATTTGACTGCGCTGACATCATCGC +CCGTGTGCGTGACATAAAACCGGTATGGGCGCTTGCCAACGACATGAACTGCAGTGCAGG +TCAGTTGCTTGCCAGTGCCGCCTCCCGGCGTCTGGTCACGCAGACCGCCCGGACAGGCTC +CATCGGCGTCATGATGGCTCACAGTAATTACGGTGCTGCGCTGGAGAAACAGGGTGTGGA +AATCACGCTGATTTACAGCGGCAGCCATAAGGTGGATGGCAACCCCTACAGCCATCTTCC +GGATGACGTCCGGGAGACACTGCAGTCCCGGATGGACGCAACCCGCCAGATGTTTGCGCA +GAAGGTGTCGGCATATACCGGCCTGTCCGTGCAGGTTGTGCTGGATACCGAGGCTGCAGT +GTACAGCGGTCAGGAGGCCATTGATGCCGGACTGGCTGATGAACTTGTTAACAGCACCGA +TGCGATCACCGTCATGCGTGATGCACTGGATGCACGTAAATCCCGTCTCTCAGGAGGGCG +AATGACCAAAGAGACTCAATCAACAACTGTTTCAGCCACTGCTTCGCAGGCTGACGTTAC +TGACGTGGTGCCAGCGACGGAGGGCGAGAACGCCAGCGCGGCGCAGCCGGACGTGAACGC +GCAGATCACCGCAGCGGTTGCGGCAGAAAACAGCCGCATTATGGGGATCCTCAACTGTGA +GGAGGCTCACGGACGCGAAGAACAGGCACGCGTGCTGGCAGAAACCCCCGGTATGACCGT +GAAAACGGCCCGCCGCATTCTGGCCGCAGCACCACAGAGTGCACAGGCGCGCAGTGACAC +TGCGCTGGATCGTCTGATGCAGGGGGCACCGGCACCGCTGGCTGCAGGTAACCCGGCATC +TGATGCCGTTAACGATTTGCTGAACACACCAGTGTAAGGGATGTTTATGACGAGCAAAGA +AACCTTTACCCATTACCAGCCGCAGGGCAACAGTGACCCGGCTCATACCGCAACCGCGCC +CGGCGGATTGAGTGCGAAAGCGCCTGCAATGACCCCGCTGATGCTGGACACCTCCAGCCG +TAAGCTGGTTGCGTGGGATGGCACCACCGACGGTGCTGCCGTTGGCATTCTTGCGGTTGC +TGCTGACCAGACCAGCACCACGCTGACGTTCTACAAGTCCGGCACGTTCCGTTATGAGGA +TGTGCTCTGGCCGGAGGCTGCCAGCGACGAGACGAAAAAACGGACCGCGTTTGCCGGAAC +GGCAATCAGCATCGTTTAACTTTACCCTTCATCACTAAAGGCCGCCTGTGCGGCTTTTTT +TACGGGATTTTTTTATGTCGATGTACACAACCGCCCAACTGCTGGCGGCAAATGAGCAGA +AATTTAAGTTTGATCCGCTGTTTCTGCGTCTCTTTTTCCGTGAGAGCTATCCCTTCACCA +CGGAGAAAGTCTATCTCTCACAAATTCCGGGACTGGTAAACATGGCGCTGTACGTTTCGC +CGATTGTTTCCGGTGAGGTTATCCGTTCCCGTGGCGGCTCCACCTCTGAATTTACGCCGG +GATATGTCAAGCCGAAGCATGAAGTGAATCCGCAGATGACCCTGCGTCGCCTGCCGGATG +AAGATCCGCAGAATCTGGCGGACCCGGCTTACCGCCGCCGTCGCATCATCATGCAGAACA +TGCGTGACGAAGAGCTGGCCATTGCTCAGGTCGAAGAGATGCAGGCAGTTTCTGCCGTGC +TTAAGGGCAAATACACCATGACCGGTGAAGCCTTCGATCCGGTTGAGGTGGATATGGGCC +GCAGTGAGGAGAATAACATCACGCAGTCCGGCGGCACGGAGTGGAGCAAGCGTGACAAGT +CCACGTATGACCCGACCGACGATATCGAAGCCTACGCGCTGAACGCCAGCGGTGTGGTGA +ATATCATCGTGTTCGATCCGAAAGGCTGGGCGCTGTTCCGTTCCTTCAAAGCCGTCAAGG +AGAAGCTGGATACCCGTCGTGGCTCTAATTCCGAGCTGGAGACAGCGGTGAAAGACCTGG +GCAAAGCGGTGTCCTATAAGGGGATGTATGGCGATGTGGCCATCGTCGTGTATTCCGGAC +AGTACGTGGAAAACGGCGTCAAAAAGAACTTCCTGCCGGACAACACGATGGTGCTGGGGA +ACACTCAGGCACGCGGTCTGCGCACCTATGGCTGCATTCAGGATGCGGACGCACAGCGCG +AAGGCATTAACGCCTCTGCCCGTTACCCGAAAAACTGGGTGACCACCGGCGATCCGGCGC +GTGAGTTCACCATGATTCAGTCAGCACCGCTGATGCTGCTGGCTGACCCTGATGAGTTCG +TGTCCGTACAACTGGCGTAATCATGGCCCTTCGGGGCCATTGTTTCTCTGTGGAGGAGTC +CATGACGAAAGATGAACTGATTGCCCGTCTCCGCTCGCTGGGTGAACAACTGAACCGTGA +TGTCAGCCTGACGGGGACGAAAGAAGAACTGGCGCTCCGTGTGGCAGAGCTGAAAGAGGA +GCTTGATGACACGGATGAAACTGCCGGTCAGGACACCCCTCTCAGCCGGGAAAATGTGCT +GACCGGACATGAAAATGAGGTGGGATCAGCGCAGCCGGATACCGTGATTCTGGATACGTC +TGAACTGGTCACGGTCGTGGCACTGGTGAAGCTGCATACTGATGCACTTCACGCCACGCG +GGATGAACCTGTGGCATTTGTGCTGCCGGGAACGGCGTTTCGTGTCTCTGCCGGTGTGGC +AGCCGAAATGACAGAGCGCGGCCTGGCCAGAATGCAATAACGGGAGGCGCTGTGGCTGAT +TTCGATAACCTGTTCGATGCTGCCATTGCCCGCGCCGATGAAACGATACGCGGGTACATG +GGAACGTCAGCCACCATTACATCCGGTGAGCAGTCAGGTGCGGTGATACGTGGTGTTTTT +GATGACCCTGAAAATATCAGCTATGCCGGACAGGGCGTGCGCGTTGAAGGCTCCAGCCCG +TCCCTGTTTGTCCGGACTGATGAGGTGCGGCAGCTGCGGCGTGGAGACACGCTGACCATC +GGTGAGGAAAATTTCTGGGTAGATCGGGTTTCGCCGGATGATGGCGGAAGTTGTCATCTC +TGGCTTGGACGGGGCGTACCGCCTGCCGTTAACCGTCGCCGCTGAAAGGGGGATGTATGG +CCATAAAAGGTCTTGAGCAGGCCGTTGAAAACCTCAGCCGTATCAGCAAAACGGCGGTGC +CTGGTGCCGCCGCAATGGCCATTAACCGCGTTGCTTCATCCGCGATATCGCAGTCGGCGT +CACAGGTTGCCCGTGAGACAAAGGTACGCCGGAAACTGGTAAAGGAAAGGGCCAGGCTGA +AAAGGGCCACGGTCAAAAATCCGCAGGCCAGAATCAAAGTTAACCGGGGGGATTTGCCCG +TAATCAAGCTGGGTAATGCGCGGGTTGTCCTTTCGCGCCGCAGGCGTCGTAAAAAGGGGC +AGCGTTCATCCCTGAAAGGTGGCGGCAGCGTGCTTGTGGTGGGTAACCGTCGTATTCCCG +GCGCGTTTATTCAGCAACTGAAAAATGGCCGGTGGCATGTCATGCAGCGTGTGGCTGGGA +AAAACCGTTACCCCATTGATGTGGTGAAAATCCCGATGGCGGTGCCGCTGACCACGGCGT +TTAAACAAAATATTGAGCGGATACGGCGTGAACGTCTTCCGAAAGAGCTGGGCTATGCGC +TGCAGCATCAACTGAGGATGGTAATAAAGCGATGAAACATACTGAACTCCGTGCAGCCGT +ACTGGATGCACTGGAGAAGCATGACACCGGGGCGACGTTTTTTGATGGTCGCCCCGCTGT +TTTTGATGAGGCGGATTTTCCGGCAGTTGCCGTTTATCTCACCGGCGCTGAATACACGGG +CGAAGAGCTGGACAGCGATACCTGGCAGGCGGAGCTGCATATCGAAGTTTTCCTGCCTGC +TCAGGTGCCGGATTCAGAGCTGGATGCGTGGATGGAGTCCCGGATTTATCCGGTGATGAG +CGATATCCCGGCACTGTCAGATTTGATCACCAGTATGGTGGCCAGCGGCTATGACTACCG +GCGCGACGATGATGCGGGCTTGTGGAGTTCAGCCGATCTGACTTATGTCATTACCTATGA +AATGTGAGGACGCTATGCCTGTACCAAATCCTACAATGCCGGTGAAAGGTGCCGGGACCA +CCCTGTGGGTTTATAAGGGGAGCGGTGACCCTTACGCGAATCCGCTTTCAGACGTTGACT +GGTCGCGTCTGGCAAAAGTTAAAGACCTGACGCCCGGCGAACTGACCGCTGAGTCCTATG +ACGACAGCTATCTCGATGATGAAGATGCAGACTGGACTGCGACCGGGCAGGGGCAGAAAT +CTGCCGGAGATACCAGCTTCACGCTGGCGTGGATGCCCGGAGAGCAGGGGCAGCAGGCGC +TGCTGGCGTGGTTTAATGAAGGCGATACCCGTGCCTATAAAATCCGCTTCCCGAACGGCA +CGGTCGATGTGTTCCGTGGCTGGGTCAGCAGTATCGGTAAGGCGGTGACGGCGAAGGAAG +TGATCACCCGCACGGTGAAAGTCACCAATGTGGGACGTCCGTCGATGGCAGAAGATCGCA +GCACGGTAACAGCGGCAACCGGCATGACCGTGACGCCTGCCAGCACCTCGGTGGTGAAAG +GGCAGAGCACCACGCTGACCGTGGCCTTCCAGCCGGAGGGCGTAACCGACAAGAGCTTTC +GTGCGGTGTCTGCGGATAAAACAAAAGCCACCGTGTCGGTCAGTGGTATGACCATCACCG +TGAACGGCGTTGCTGCAGGCAAGGTCAACATTCCGGTTGTATCCGGTAATGGTGAGTTTG +CTGCGGTTGCAGAAATTACCGTCACCGCCAGTTAATCCGGAGAGTCAGCGATGTTCCTGA +AAACCGAATCATTTGAACATAACGGTGTGACCGTCACGCTTTCTGAACTGTCAGCCCTGC +AGCGCATTGAGCATCTCGCCCTGATGAAACGGCAGGCAGAACAGGCGGAGTCAGACAGCA +ACCGGAAGTTTACTGTGGAAGACGCCATCAGAACCGGCGCGTTTCTGGTGGCGATGTCCC +TGTGGCATAACCATCCGCAGAAGACGCAGATGCCGTCCATGAATGAAGCCGTTAAACAGA +TTGAGCAGGAAGTGCTTACCACCTGGCCCACGGAGGCAATTTCTCATGCTGAAAACGTGG +TGTACCGGCTGTCTGGTATGTATGAGTTTGTGGTGAATAATGCCCCTGAACAGACAGAGG +ACGCCGGGCCCGCAGAGCCTGTTTCTGCGGGAAAGTGTTCGACGGTGAGCTGAGTTTTGC +CCTGAAACTGGCGCGTGAGATGGGGCGACCCGACTGGCGTGCCATGCTTGCCGGGATGTC +ATCCACGGAGTATGCCGACTGGCACCGCTTTTACAGTACCCATTATTTTCATGATGTTCT +GCTGGATATGCACTTTTCCGGGCTGACGTACACCGTGCTCAGCCTGTTTTTCAGCGATCC +GGATATGCATCCGCTGGATTTCAGTCTGCTGAACCGGCGCGAGGCTGACGAAGAGCCTGA +AGATGATGTGCTGATGCAGAAAGCGGCAGGGCTTGCCGGAGGTGTCCGCTTTGGCCCGGA +CGGGAATGAAGTTATCCCCGCTTCCCCGGATGTGGCGGACATGACGGAGGATGACGTAAT +GCTGATGACAGTATCAGAAGGGATCGCAGGAGGAGTCCGGTATGGCTGAACCGGTAGGCG +ATCTGGTCGTTGATTTGAGTCTGGATGCGGCCAGATTTGACGAGCAGATGGCCAGAGTCA +GGCGTCATTTTTCTGGTACGGAAAGTGATGCGAAAAAAACAGCGGCAGTCGTTGAACAGT +CGCTGAGCCGACAGGCGCTGGCTGCACAGAAAGCGGGGATTTCCGTCGGGCAGTATAAAG +CCGCCATGCGTATGCTGCCTGCACAGTTCACCGACGTGGCCACGCAGCTTGCAGGCGGGC +AAAGTCCGTGGCTGATCCTGCTGCAACAGGGGGGGCAGGTGAAGGACTCCTTCGGCGGGA +TGATCCCCATGTTCAGGGGGCTTGCCGGTGCGATCACCCTGCCGATGGTGGGGGCCACCT +CGCTGGCGGTGGCGACCGGTGCGCTGGCGTATGCCTGGTATCAGGGCAACTCAACCCTGT +CCGATTTCAACAAAACGCTGGTCCTTTCCGGCAATCAGGCGGGACTGACGGCAGATCGTA +TGCTGGTCCTGTCCAGAGCCGGGCAGGCGGCAGGGCTGACGTTTAACCAGACCAGCGAGT +CACTCAGCGCACTGGTTAAGGCGGGGGTAAGCGGTGAGGCTCAGATTGCGTCCATCAGCC +AGAGTGTGGCGCGTTTCTCCTCTGCATCCGGCGTGGAGGTGGACAAGGTCGCTGAAGCCT +TCGGGAAGCTGACCACAGACCCGACGTCGGGGCTGACGGCGATGGCTCGCCAGTTCCATA +ACGTGTCGGCGGAGCAGATTGCGTATGTTGCTCAGTTGCAGCGTTCCGGCGATGAAGCCG +GGGCATTGCAGGCGGCGAACGAGGCCGCAACGAAAGGGTTTGATGACCAGACCCGCCGCC +TGAAAGAGAACATGGGCACGCTGGAGACCTGGGCAGACAGGACTGCGCGGGCATTCAAAT +CCATGTGGGATGCGGTGCTGGATATTGGTCGTCCTGATACCGCGCAGGAGATGCTGATTA +AGGCAGAGGCTGCGTATAAGAAAGCAGACGACATCTGGAATCTGCGCAAGGATGATTATT +TTGTTAACGATGAAGCGCGGGCGCGTTACTGGGATGATCGTGAAAAGGCCCGTCTTGCGC +TTGAAGCCGCCCGAAAGAAGGCTGAGCAGCAGACTCAACAGGACAAAAATGCGCAGCAGC +AGAGCGATACCGAAGCGTCACGGCTGAAATATACCGAAGAGGCGCAGAAGGCTTACGAAC +GGCTGCAGACGCCGCTGGAGAAATATACCGCCCGTCAGGAAGAACTGAACAAGGCACTGA +AAGACGGGAAAATCCTGCAGGCGGATTACAACACGCTGATGGCGGCGGCGAAAAAGGATT +ATGAAGCGACGCTGAAAAAGCCGAAACAGTCCAGCGTGAAGGTGTCTGCGGGCGATCGTC +AGGAAGACAGTGCTCATGCTGCCCTGCTGACGCTTCAGGCAGAACTCCGGACGCTGGAGA +AGCATGCCGGAGCAAATGAGAAAATCAGCCAGCAGCGCCGGGATTTGTGGAAGGCGGAGA +GTCAGTTCGCGGTACTGGAGGAGGCGGCGCAACGTCGCCAGCTGTCTGCACAGGAGAAAT +CCCTGCTGGCGCATAAAGATGAGACGCTGGAGTACAAACGCCAGCTGGCTGCACTTGGCG +ACAAGGTTACGTATCAGGAGCGCCTGAACGCGCTGGCGCAGCAGGCGGATAAATTCGCAC +AGCAGCAACGGGCAAAACGGGCCGCCATTGATGCGAAAAGCCGGGGGCTGACTGACCGGC +AGGCAGAACGGGAAGCCACGGAACAGCGCCTGAAGGAACAGTATGGCGATAATCCGCTGG +CGCTGAATAACGTCATGTCAGAGCAGAAAAAGACCTGGGCGGCTGAAGACCAGCTTCGCG +GGAACTGGATGGCAGGCCTGAAGTCCGGCTGGAGTGAGTGGGAAGAGAGCGCCACGGACA +GTATGTCGCAGGTAAAAAGTGCAGCCACGCAGACCTTTGATGGTATTGCACAGAATATGG +CGGCGATGCTGACCGGCAGTGAGCAGAACTGGCGCAGCTTCACCCGTTCCGTGCTGTCCA +TGATGACAGAAATTCTGCTTAAGCAGGCAATGGTGGGGATTGTCGGGAGTATCGGCAGCG +CCATTGGCGGGGCTGTTGGTGGCGGCGCATCCGCGTCAGGCGGTACAGCCATTCAGGCCG +CTGCGGCGAAATTCCATTTTGCAACCGGAGGATTTACGGGAACCGGCGGCAAATATGAGC +CAGCGGGGATTGTTCACCGTGGTGAGTTTGTCTTCACGAAGGAGGCAACCAGCCGGATTG +GCGTGGGGAATCTTTACCGGCTGATGCGCGGCTATGCCACCGGCGGTTATGTCGGTACAC +CGGGCAGCATGGCAGACAGCCGGTCGCAGGCGTCCGGGACGTTTGAGCAGAATAACCATG +TGGTGATTAACAACGACGGCACGAACGGGCAGATAGGTCCGGCTGCTCTGAAGGCGGTGT +ATGACATGGCCCGCAAGGGTGCCCGTGATGAAATTCAGACACAGATGCGTGATGGTGGCC +TGTTCTCCGGAGGTGGACGATGAAGACCTTCCGCTGGAAAGTGAAACCCGGTATGGATGT +GGCTTCGGTCCCTTCTGTAAGAAAGGTGCGCTTTGGTGATGGCTATTCTCAGCGAGCGCC +TGCCGGGCTGAATGCCAACCTGAAAACGTACAGCGTGACGCTTTCTGTCCCCCGTGAGGA +GGCCACGGTACTGGAGTCGTTTCTGGAAGAGCACGGGGGCTGGAAATCCTTTCTGTGGAC +GCCGCCTTATGAGTGGCGGCAGATAAAGGTGACCTGCGCAAAATGGTCGTCGCGGGTCAG +TATGCTGCGTGTTGAGTTCAGCGCAGAGTTTGAACAGGTGGTGAACTGATGCAGGATATC +CGGCAGGAAACACTGAATGAATGCACCCGTGCGGAGCAGTCGGCCAGCGTGGTGCTCTGG +GAAATCGACCTGACAGAGGTCGGTGGAGAACGTTATTTTTTCTGTAATGAGCAGAACGAA +AAAGGTGAGCCGGTCACCTGGCAGGGGCGACAGTATCAGCCGTATCCCATTCAGGGGAGC +GGTTTTGAACTGAATGGCAAAGGCACCAGTACGCGCCCCACGCTGACGGTTTCTAACCTG +TACGGTATGGTCACCGGGATGGCGGAAGATATGCAGAGTCTGGTCGGCGGAACGGTGGTC +CGGCGTAAGGTTTACGCCCGTTTTCTGGATGCGGTGAACTTCGTCAACGGAAACAGTTAC +GCCGATCCGGAGCAGGAGGTGATCAGCCGCTGGCGCATTGAGCAGTGCAGCGAACTGAGC +GCGGTGAGTGCCTCCTTTGTACTGTCCACGCCGACGGAAACGGATGGCGCTGTTTTTCCG +GGACGTATCATGCTGGCCAACACCTGCACCTGGACCTATCGCGGTGACGAGTGCGGTTAT +AGCGGTCCGGCTGTCGCGGATGAATATGACCAGCCAACGTCCGATATCACGAAGGATAAA +TGCAGCAAATGCCTGAGCGGTTGTAAGTTCCGCAATAACGTCGGCAACTTTGGCGGCTTC +CTTTCCATTAACAAACTTTCGCAGTAAATCCCATGACACAGACAGAATCAGCGATTCTGG +CGCACGCCCGGCGATGTGCGCCAGCGGAGTCGTGCGGCTTCGTGGTAAGCACGCCGGAGG +GGGAAAGATATTTCCCCTGCGTGAATATCTCCGGTGAGCCGGAGGCTATTTCCGTATGTC +GCCGGAAGACTGGCTGCAGGCAGAAATGCAGGGTGAGATTGTGGCGCTGGTCCACAGCCA +CCCCGGTGGTCTGCCCTGGCTGAGTGAGGCCGACCGGCGGCTGCAGGTGCAGAGTGATTT +GCCGTGGTGGCTGGTCTGCCGGGGGACGATTCATAAGTTCCGCTGTGTGCCGCATCTCAC +CGGGCGGCGCTTTGAGCACGGTGTGACGGACTGTTACACACTGTTCCGGGATGCTTATCA +TCTGGCGGGGATTGAGATGCCGGACTTTCATCGTGAGGATGACTGGTGGCGTAACGGCCA +GAATCTCTATCTGGATAATCTGGAGGCGACGGGGCTGTATCAGGTGCCGTTGTCAGCGGC +ACAGCCGGGCGATGTGCTGCTGTGCTGTTTTGGTTCATCAGTGCCGAATCACGCCGCAAT +TTACTGCGGCGACGGCGAGCTGCTGCACCATATTCCTGAACAACTGAGCAAACGAGAGAG +GTACACCGACAAATGGCAGCGACGCACACACTCCCTCTGGCGTCACCGGGCATGGCGCGC +ATCTGCCTTTACGGGGATTTACAACGATTTGGTCGCCGCATCGACCTTCGTGTGAAAACG +GGGGCTGAAGCCATCCGGGCACTGGCCACACAGCTCCCGGCGTTTCGTCAGAAACTGAGC +GACGGCTGGTATCAGGTACGGATTGCCGGGCGGGACGTCAGCACGTCCGGGTTAACGGCG +CAGTTACATGAGACTCTGCCTGATGGCGCTGTAATTCATATTGTTCCCAGAGTCGCCGGG +GCCAAGTCAGGTGGCGTATTCCAGATTGTCCTGGGGGCTGCCGCCATTGCCGGATCATTC +TTTACCGCCGGAGCCACCCTTGCAGCATGGGGGGCAGCCATTGGGGCCGGTGGTATGACC +GGCATCCTGTTTTCTCTCGGTGCCAGTATGGTGCTCGGTGGTGTGGCGCAGATGCTGGCA +CCGAAAGCCAGAACTCCCCGTATACAGACAACGGATAACGGTAAGCAGAACACCTATTTC +TCCTCACTGGATAACATGGTTGCCCAGGGCAATGTTCTGCCTGTTCTGTACGGGGAAATG +CGCGTGGGGTCACGCGTGGTTTCTCAGGAGATCAGCACGGCAGACGAAGGGGACGGTGGT +CAGGTTGTGGTGATTGGTCGCTGATGCAAAATGTTTTATGTGAAACCGCCTGCGGGCGGT +TTTGTCATTTATGGAGCGTGAGGAATGGGTAAAGGAAGCAGTAAGGGGCATACCCCGCGC +GAAGCGAAGGACAACCTGAAGTCCACGCAGTTGCTGAGTGTGATCGATGCCATCAGCGAA +GGGCCGATTGAAGGTCCGGTGGATGGCTTAAAAAGCGTGCTGCTGAACAGTACGCCGGTG +CTGGACACTGAGGGGAATACCAACATATCCGGTGTCACGGTGGTGTTCCGGGCTGGTGAG +CAGGAGCAGACTCCGCCGGAGGGATTTGAATCCTCCGGCTCCGAGACGGTGCTGGGTACG +GAAGTGAAATATGACACGCCGATCACCCGCACCATTACGTCTGCAAACATCGACCGTCTG +CGCTTTACCTTCGGTGTACAGGCACTGGTGGAAACCACCTCAAAGGGTGACAGGAATCCG +TCGGAAGTCCGCCTGCTGGTTCAGATACAACGTAACGGTGGCTGGGTGACGGAAAAAGAC +ATCACCATTAAGGGCAAAACCACCTCGCAGTATCTGGCCTCGGTGGTGATGGGTAACCTG +CCGCCGCGCCCGTTTAATATCCGGATGCGCAGGATGACGCCGGACAGCACCACAGACCAG +CTGCAGAACAAAACGCTCTGGTCGTCATACACTGAAATCATCGATGTGAAACAGTGCTAC +CCGAACACGGCACTGGTCGGCGTGCAGGTGGACTCGGAGCAGTTCGGCAGCCAGCAGGTG +AGCCGTAATTATCATCTGCGCGGGCGTATTCTGCAGGTGCCGTCGAACTATAACCCGCAG +ACGCGGCAATACAGCGGTATCTGGGACGGAACGTTTAAACCGGCATACAGCAACAACATG +GCCTGGTGTCTGTGGGATATGCTGACCCATCCGCGCTACGGCATGGGGAAACGTCTTGGT +GCGGCGGATGTGGATAAATGGGCGCTGTATGTCATCGGCCAGTACTGCGACCAGTCAGTG +CCGGACGGCTTTGGCGGCACGGAGCCGCGCATCACCTGTAATGCGTACCTGACCACACAG +CGTAAGGCGTGGGATGTGCTCAGCGATTTCTGCTCGGCGATGCGCTGTATGCCGGTATGG +AACGGGCAGACGCTGACGTTCGTGCAGGACCGACCGTCGGATAAGACGTGGACCTATAAC +CGCAGTAATGTGGTGATGCCGGATGATGGCGCGCCGTTCCGCTACAGCTTCAGCGCCCTG +AAGGACCGCCATAATGCCGTTGAGGTGAACTGGATTGACCCGAACAACGGCTGGGAGACG +GCGACAGAGCTTGTTGAAGATACGCAGGCCATTGCCCGTTACGGTCGTAATGTTACGAAG +ATGGATGCCTTTGGCTGTACCAGCCGGGGGCAGGCACACCGCGCCGGGCTGTGGCTGATT +AAAACAGAACTGCTGGAAACGCAGACCGTGGATTTCAGCGTCGGCGCAGAAGGGCTTCGC +CATGTACCGGGCGATGTTATTGAAATCTGCGATGATGACTATGCCGGTATCAGCACCGGT +GGTCGTGTGCTGGCGGTGAACAGCCAGACCCGGACGCTGACGCTCGACCGTGAAATCACG +CTGCCATCCTCCGGTACCGCGCTGATAAGCCTGGTTGACGGAAGTGGCAATCCGGTCAGC +GTGGAGGTTCAGTCCGTCACCGACGGCGTGAAGGTAAAAGTGAGCCGTGTTCCTGACGGT +GTTGCTGAATACAGCGTATGGGAGCTGAAGCTGCCGACGCTGCGCCAGCGACTGTTCCGC +TGCGTGAGTATCCGTGAGAACGACGACGGCACGTATGCCATCACCGCCGTGCAGCATGTG +CCGGAAAAAGAGGCCATCGTGGATAACGGGGCGCACTTTGACGGCGAACAGAGTGGCACG +GTGAATGGTGTCACGCCGCCAGCGGTGCAGCACCTGACCGCAGAAGTCACTGCAGACAGC +GGGGAATATCAGGTGCTGGCGCGATGGGACACACCGAAGGTGGTGAAGGGCGTGAGTTTC +CTGCTCCGTCTGACCGTAACAGCGGACGACGGCAGTGAGCGGCTGGTCAGCACGGCCCGG +ACGACGGAAACCACATACCGCTTCACGCAACTGGCGCTGGGGAACTACAGGCTGACAGTC +CGGGCGGTAAATGCGTGGGGGCAGCAGGGCGATCCGGCGTCGGTATCGTTCCGGATTGCC +GCACCGGCAGCACCGTCGAGGATTGAGCTGACGCCGGGCTATTTTCAGATAACCGCCACG +CCGCATCTTGCCGTTTATGACCCGACGGTACAGTTTGAGTTCTGGTTCTCGGAAAAGCAG +ATTGCGGATATCAGACAGGTTGAAACCAGCACGCGTTATCTTGGTACGGCGCTGTACTGG +ATAGCCGCCAGTATCAATATCAAACCGGGCCATGATTATTACTTTTATATCCGCAGTGTG +AACACCGTTGGCAAATCGGCATTCGTGGAGGCCGTCGGTCGGGCGAGCGATGATGCGGAA +GGTTACCTGGATTTTTTCAAAGGCAAGATAACCGAATCCCATCTCGGCAAGGAGCTGCTG +GAAAAAGTCGAGCTGACGGAGGATAACGCCAGCAGACTGGAGGAGTTTTCGAAAGAGTGG +AAGGATGCCAGTGATAAGTGGAATGCCATGTGGGCTGTCAAAATTGAGCAGACCAAAGAC +GGCAAACATTATGTCGCGGGTATTGGCCTCAGCATGGAGGACACGGAGGAAGGCAAACTG +AGCCAGTTTCTGGTTGCCGCCAATCGTATCGCATTTATTGACCCGGCAAACGGGAATGAA +ACGCCGATGTTTGTGGCGCAGGGCAACCAGATATTCATGAACGACGTGTTCCTGAAGCGC +CTGACGGCCCCCACCATTACCAGCGGCGGCAATCCTCCGGCCTTTTCCCTGACACCGGAC +GGAAAGCTGACCGCTAAAAATGCGGATATCAGTGGCAGTGTGAATGCGAACTCCGGGACG +CTCAGTAATGTGACGATAGCTGAAAACTGTACGATAAACGGTACGCTGAGGGCGGAAAAA +ATCGTCGGGGACATTGTAAAGGCGGCGAGCGCGGCTTTTCCGCGCCAGCGTGAAAGCAGT +GTGGACTGGCCGTCAGGTACCCGTACTGTCACCGTGACCGATGACCATCCTTTTGATCGC +CAGATAGTGGTGCTTCCGCTGACGTTTCGCGGAAGTAAGCGTACTGTCAGCGGCAGGACA +ACGTATTCGATGTGTTATCTGAAAGTACTGATGAACGGTGCGGTGATTTATGATGGCGCG +GCGAACGAGGCGGTACAGGTGTTCTCCCGTATTGTTGACATGCCAGCGGGTCGGGGAAAC +GTGATCCTGACGTTCACGCTTACGTCCACACGGCATTCGGCAGATATTCCGCCGTATACG +TTTGCCAGCGATGTGCAGGTTATGGTGATTAAGAAACAGGCGCTGGGCATCAGCGTGGTC +TGAGTGTGTTACAGAGGTTCGTCCGGGAACGGGCGTTTTATTATAAAACAGTGAGAGGTG +AACGATGCGTAATGTGTGTATTGCCGTTGCTGTCTTTGCCGCACTTGCGGTGACAGTCAC +TCCGGCCCGTGCGGAAGGTGGACATGGTACGTTTACGGTGGGCTATTTTCAAGTGAAACC +GGGTACATTGCCGTCGTTGTCGGGCGGGGATACCGGTGTGAGTCATCTGAAAGGGATTAA +CGTGAAGTACCGTTATGAGCTGACGGACAGTGTGGGGGTGATGGCTTCCCTGGGGTTCGC +CGCGTCGAAAAAGAGCAGCACAGTGATGACCGGGGAGGATACGTTTCACTATGAGAGCCT +GCGTGGACGTTATGTGAGCGTGATGGCCGGACCGGTTTTACAAATCAGTAAGCAGGTCAG +TGCGTACGCCATGGCCGGAGTGGCTCACAGTCGGTGGTCCGGCAGTACAATGGATTACCG +TAAGACGGAAATCACTCCCGGGTATATGAAAGAGACGACCACTGCCAGGGACGAAAGTGC +AATGCGGCATACCTCAGTGGCGTGGAGTGCAGGTATACAGATTAATCCGGCAGCGTCCGT +CGTTGTTGATATTGCTTATGAAGGCTCCGGCAGTGGCGACTGGCGTACTGACGGATTCAT +CGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCG +GAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAG +ACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCA +CCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCA +TGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGC +ACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCT +GTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGG +AAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAG +CCGGCGATGCCAGTGCATCAGCTGCTCAGGTCGCGGCCCTTGTGACTGATGCAACTGACT +CAGCACGCGCCGCCAGCACGTCCGCCGGACAGGCTGCATCGTCAGCTCAGGAAGCGTCCT +CCGGCGCAGAAGCGGCATCAGCAAAGGCCACTGAAGCGGAAAAAAGTGCCGCAGCCGCAG +AGTCCTCAAAAAACGCGGCGGCCACCAGTGCCGGTGCGGCGAAAACGTCAGAAACGAATG +CTGCAGCGTCACAACAATCAGCCGCCACGTCTGCCTCCACCGCGGCCACGAAAGCGTCAG +AGGCCGCCACTTCAGCACGAGATGCGGTGGCCTCAAAAGAGGCAGCAAAATCATCAGAAA +CGAACGCATCATCAAGTGCCGGTCGTGCAGCTTCCTCGGCAACGGCGGCAGAAAATTCTG +CCAGGGCGGCAAAAACGTCCGAGACGAATGCCAGGTCATCTGAAACAGCAGCGGAACGGA +GCGCCTCTGCCGCGGCAGACGCAAAAACAGCGGCGGCGGGGAGTGCGTCAACGGCATCCA +CGAAGGCGACAGAGGCTGCGGGAAGTGCGGTATCAGCATCGCAGAGCAAAAGTGCGGCAG +AAGCGGCGGCAATACGTGCAAAAAATTCGGCAAAACGTGCAGAAGATATAGCTTCAGCTG +TCGCGCTTGAGGATGCGGACACAACGAGAAAGGGGATAGTGCAGCTCAGCAGTGCAACCA +ACAGCACGTCTGAAACGCTTGCTGCAACGCCAAAGGCGGTTAAGGTGGTAATGGATGAAA +CGAACAGAAAAGCCCACTGGACAGTCCGGCACTGACCGGAACGCCAACAGCACCAACCGC +GCTCAGGGGAACAAACAATACCCAGATTGCGAACACCGCTTTTGTACTGGCCGCGATTGC +AGATGTTATCGACGCGTCACCTGACGCACTGAATACGCTGAATGAACTGGCCGCAGCGCT +CGGGAATGATCCAGATTTTGCTACCACCATGACTAACGCGCTTGCGGGTAAACAACCGAA +GAATGCGACACTGACGGCGCTGGCAGGGCTTTCCACGGCGAAAAATAAATTACCGTATTT +TGCGGAAAATGATGCCGCCAGCCTGACTGAACTGACTCAGGTTGGCAGGGATATTCTGGC +AAAAAATTCCGTTGCAGATGTTCTTGAATACCTTGGGGCCGGTGAGAATTCGGCCTTTCC +GGCAGGTGCGCCGATCCCGTGGCCATCAGATATCGTTCCGTCTGGCTACGTCCTGATGCA +GGGGCAGGCGTTTGACAAATCAGCCTACCCAAAACTTGCTGTCGCGTATCCATCGGGTGT +GCTTCCTGATATGCGAGGCTGGACAATCAAGGGGAAACCCGCCAGCGGTCGTGCTGTATT +GTCTCAGGAACAGGATGGAATTAAGTCGCACACCCACAGTGCCAGTGCATCCGGTACGGA +TTTGGGGACGAAAACCACATCGTCGTTTGATTACGGGACGAAAACAACAGGCAGTTTCGA +TTACGGCACCAAATCGACGAATAACACGGGGGCTCATGCTCACAGTCTGAGCGGTTCAAC +AGGGGCCGCGGGTGCTCATGCCCACACAAGTGGTTTAAGGATGAACAGTTCTGGCTGGAG +TCAGTATGGAACAGCAACCATTACAGGAAGTTTATCCACAGTTAAAGGAACCAGCACACA +GGGTATTGCTTATTTATCGAAAACGGACAGTCAGGGCAGCCACAGTCACTCATTGTCCGG +TACAGCCGTGAGTGCCGGTGCACATGCGCATACAGTTGGTATTGGTGCGCACCAGCATCC +GGTTGTTATCGGTGCTCATGCCCATTCTTTCAGTATTGGTTCACACGGACACACCATCAC +CGTTAACGCTGCGGGTAACGCGGAAAACACCGTCAAAAACATTGCATTTAACTATATTGT +GAGGCTTGCATAATGGCATTCAGAATGAGTGAACAACCACGGACCATAAAAATTTATAAT +CTGCTGGCCGGAACTAATGAATTTATTGGTGAAGGTGACGCATATATTCCGCCTCATACC +GGTCTGCCTGCAAACAGTACCGATATTGCACCGCCAGATATTCCGGCTGGCTTTGTGGCT +GTTTTCAACAGTGATGAGGCATCGTGGCATCTCGTTGAAGACCATCGGGGTAAAACCGTC +TATGACGTGGCTTCCGGCGACGCGTTATTTATTTCTGAACTCGGTCCGTTACCGGAAAAT +TTTACCTGGTTATCGCCGGGAGGGGAATATCAGAAGTGGAACGGCACAGCCTGGGTGAAG +GATACGGAAGCAGAAAAACTGTTCCGGATCCGGGAGGCGGAAGAAACAAAAAAAAGCCTG +ATGCAGGTAGCCAGTGAGCATATTGCGCCGCTTCAGGATGCTGCAGATCTGGAAATTGCA +ACGAAGGAAGAAACCTCGTTGCTGGAAGCCTGGAAGAAGTATCGGGTGTTGCTGAACCGT +GTTGATACATCAACTGCACCTGATATTGAGTGGCCTGCTGTCCCTGTTATGGAGTAATCG +TTTTGTGATATGCCGCAGAAACGTTGTATGAAATAACGTTCTGCGGTTAGTTAGTATATT +GTAAAGCTGAGTATTGGTTTATTTGGCGATTATTATCTTCAGGAGAATAATGGAAGTTCT +ATGACTCAATTGTTCATAGTGTTTACATCACCGCCAATTGCTTTTAAGACTGAACGCATG +AAATATGGTTTTTCGTCATGTTTTGAGTCTGCTGTTGATATTTCTAAAGTCGGTTTTTTT +TCTTCGTTTTCTCTAACTATTTTCCATGAAATACATTTTTGATTATTATTTGAATCAATT +CCAATTACCTGAAGTCTTTCATCTATAATTGGCATTGTATGTATTGGTTTATTGGAGTAG +ATGCTTGCTTTTCTGAGCCATAGCTCTGATATCCAAATGAAGCCATAGGCATTTGTTATT +TTGGCTCTGTCAGCTGCATAACGCCAAAAAATATATTTATCTGCTTGATCTTCAAATGTT +GTATTGATTAAATCAATTGGATGGAATTGTTTATCATAAAAAATTAATGTTTGAATGTGA +TAACCGTCCTTTAAAAAAGTCGTTTCTGCAAGCTTGGCTGTATAGTCAACTAACTCTTCT +GTCGAAGTGATATTTTTAGGCTTATCTACCAGTTTTAGACGCTCTTTAATATCTTCAGGA +ATTATTTTATTGTCATATTGTATCATGCTAAATGACAATTTGCTTATGGAGTAATCTTTT +AATTTTAAATAAGTTATTCTCCTGGCTTCATCAAATAAAGAGTCGAATGATGTTGGCGAA +ATCACATCGTCACCCATTGGATTGTTTATTTGTATGCCAAGAGAGTTACAGCAGTTATAC +ATTCTGCCATAGATTATAGCTAAGGCATGTAATAATTCGTAATCTTTTAGCGTATTAGCG +ACCCATCGTCTTTCTGATTTAATAATAGATGATTCAGTTAAATATGAAGGTAATTTCTTT +TGTGCAAGTCTGACTAACTTTTTTATACCAATGTTTAACATACTTTCATTTGTAATAAAC +TCAATGTCATTTTCTTCAATGTAAGATGAAATAAGAGTAGCCTTTGCCTCGCTATACATT +TCTAAATCGCCTTGTTTTTCTATCGTATTGCGAGAATTTTTAGCCCAAGCCATTAATGGA +TCATTTTTCCATTTTTCAATAACATTATTGTTATACCAAATGTCATATCCTATAATCTGG +TTTTTGTTTTTTTGAATAATAAATGTTACTGTTCTTGCGGTTTGGAGGAATTGATTCAAA +TTCAAGCGAAATAATTCAGGGTCAAAATATGTATCAATGCAGCATTTGAGCAAGTGCGAT +AAATCTTTAAGTCTTCTTTCCCATGGTTTTTTAGTCATAAAACTCTCCATTTTGATAGGT +TGCATGCTAGATGCTGATATATTTTAGAGGTGATAAAATTAACTGCTTAACTGTCAATGT +AATACAAGTTGTTTGATCTTTGCAATGATTCTTATCAGAAACCATATAGTAAATTAGTTA +CACAGGAAATTTTTAATATTATTATTATCATTCATTATGTATTAAAATTAGAGTTGTGGC +TTGGCTCTGCTAACACGTTGCTCATAGGAGATATGGTAGAGCCGCAGACACGTCGTATGC +AGGAACGTGCTGCGGCTGGCTGGTGAACTTCCGATAGTGCGGGTGTTGAATGATTTCCAG +TTGCTACCGATTTTACATATTTTTTGCATGAGAGAATTTGTACCACCTCCCACCGACCAT +CTATGACTGTACGCCACTGTCCCTAGGACTGCTATGTGCCGGAGCGGACATTACAAACGT +CCTTCTCGGTGCATGCCACTGTTGCCAATGACCTGCCTAGGAATTGGTTAGCAAGTTACT +ACCGGATTTTGTAAAAACAGCCCTCCTCATATAAAAAGTATTCGTTCACTTCCGATAAGC +GTCGTAATTTTCTATCTTTCATCATATTCTAGATCCCTCTGAAAAAATCTTCCGAGTTTG +CTAGGCACTGATACATAACTCTTTTCCAATAATTGGGGAAGTCATTCAAATCTATAATAG +GTTTCAGATTTGCTTCAATAAATTCTGACTGTAGCTGCTGAAACGTTGCGGTTGAACTAT +ATTTCCTTATAACTTTTACGAAAGAGTTTCTTTGAGTAATCACTTCACTCAAGTGCTTCC +CTGCCTCCAAACGATACCTGTTAGCAATATTTAATAGCTTGAAATGATGAAGAGCTCTGT +GTTTGTCTTCCTGCCTCCAGTTCGCCGGGCATTCAACATAAAAACTGATAGCACCCGGAG +TTCCGGAAACGAAATTTGCATATACCCATTGCTCACGAAAAAAAATGTCCTTGTCGATAT +AGGGATGAATCGCTTGGTGTACCTCATCTACTGCGAAAACTTGACCTTTCTCTCCCATAT +TGCAGTCGCGGCACGATGGAACTAAATTAATAGGCATCACCGAAAATTCAGGATAATGTG +CAATAGGAAGAAAATGATCTATATTTTTTGTCTGTCCTATATCACCACAAAATGGACATT +TTTCACCTGATGAAACAAGCATGTCATCGTAATATGTTCTAGCGGGTTTGTTTTTATCTC +GGAGATTATTTTCATAAAGCTTTTCTAATTTAACCTTTGTCAGGTTACCAACTACTAAGG +TTGTAGGCTCAAGAGGGTGTGTCCTGTCGTAGGTAAATAACTGACCTGTCGAGCTTAATA +TTCTATATTGTTGTTCTTTCTGCAAAAAAGTGGGGAAGTGAGTAATGAAATTATTTCTAA +CATTTATCTGCATCATACCTTCCGAGCATTTATTAAGCATTTCGCTATAAGTTCTCGCTG +GAAGAGGTAGTTTTTTCATTGTACTTTACCTTCATCTCTGTTCATTATCATCGCTTTTAA +AACGGTTCGACCTTCTAATCCTATCTGACCATTATAATTTTTTAGAATGGTTTCATAAGA +AAGCTCTGAATCAACGGACTGCGATAATAAGTGGTGGTATCCAGAATTTGTCACTTCAAG +TAAAAACACCTCACGAGTTAAAACACCTAAGTTCTCACCGAATGTCTCAATATCCGGACG +GATAATATTTATTGCTTCTCTTGACCGTAGGACTTTCCACATGCAGGATTTTGGAACCTC +TTGCAGTACTACTGGGGAATGAGTTGCAATTATTGCTACACCATTGCGTGCATCGAGTAA +GTCGCTTAATGTTCGTAAAAAAGCAGAGAGCAAAGGTGGATGCAGATGAACCTCTGGTTC +ATCGAATAAAACTAATGACTTTTCGCCAACGACATCTACTAATCTTGTGATAGTAAATAA +AACAATTGCATGTCCAGAGCTCATTCGAAGCAGATATTTCTGGATATTGTCATAAAACAA +TTTAGTGAATTTATCATCGTCCACTTGAATCTGTGGTTCATTACGTCTTAACTCTTCATA +TTTAGAAATGAGGCTGATGAGTTCCATATTTGAAAAGTTTTCATCACTACTTAGTTTTTT +GATAGCTTCAAGCCAGAGTTGTCTTTTTCTATCTACTCTCATACAACCAATAAATGCTGA +AATGAATTCTAAGCGGAGATCGCCTAGTGATTTTAAACTATTGCTGGCAGCATTCTTGAG +TCCAATATAAAAGTATTGTGTACCTTTTGCTGGGTCAGGTTGTTCTTTAGGAGGAGTAAA +AGGATCAAATGCACTAAACGAAACTGAAACAAGCGATCGAAAATATCCCTTTGGGATTCT +TGACTCGATAAGTCTATTATTTTCAGAGAAAAAATATTCATTGTTTTCTGGGTTGGTGAT +TGCACCAATCATTCCATTCAAAATTGTTGTTTTACCACACCCATTCCGCCCGATAAAAGC +ATGAATGTTCGTGCTGGGCATAGAATTAACCGTCACCTCAAAAGGTATAGTTAAATCACT +GAATCCGGGAGCACTTTTTCTATTAAATGAAAAGTGGAAATCTGACAATTCTGGCAAACC +ATTTAACACACGTGCGAACTGTCCATGAATTTCTGAAAGAGTTACCCCTCTAAGTAATGA +GGTGTTAAGGACGCTTTCATTTTCAATGTCGGCTAATCGATTTGGCCATACTACTAAATC +CTGAATAGCTTTAAGAAGGTTATGTTTAAAACCATCGCTTAATTTGCTGAGATTAACATA +GTAGTCAATGCTTTCACCTAAGGAAAAAAACATTTCAGGGAGTTGACTGAATTTTTTATC +TATTAATGAATAAGTGCTTACTTCTTCTTTTTGACCTACAAAACCAATTTTAACATTTCC +GATATCGCATTTTTCACCATGCTCATCAAAGACAGTAAGATAAAACATTGTAACAAAGGA +ATAGTCATTCCAACCATCTGCTCGTAGGAATGCCTTATTTTTTTCTACTGCAGGAATATA +CCCGCCTCTTTCAATAACACTAAACTCCAACATATAGTAACCCTTAATTTTATTAAAATA +ACCGCAATTTATTTGGCGGCAACACAGGATCTCTCTTTTAAGTTACTCTCTATTACATAC +GTTTTCCATCTAAAAATTAGTAGTATTGAACTTAACGGGGCATCGTATTGTAGTTTTCCA +TATTTAGCTTTCTGCTTCCTTTTGGATAACCCACTGTTATTCATGTTGCATGGTGCACTG +TTTATACCAACGATATAGTCTATTAATGCATATATAGTATCGCCGAACGATTAGCTCTTC +AGGCTTCTGAAGAAGCGTTTCAAGTACTAATAAGCCGATAGATAGCCACGGACTTCGTAG +CCATTTTTCATAAGTGTTAACTTCCGCTCCTCGCTCATAACAGACATTCACTACAGTTAT +GGCGGAAAGGTATGCATGCTGGGTGTGGGGAAGTCGTGAAAGAAAAGAAGTCAGCTGCGT +CGTTTGACATCACTGCTATCTTCTTACTGGTTATGCAGGTCGTAGTGGGTGGCACACAAA +GCTTTGCACTGGATTGCGAGGCTTTGTGCTTCTCTGGAGTGCGACAGGTTTGATGACAAA +AAATTAGCGCAAGAAGACAAAAATCACCTTGCGCTAATGCTCTGTTACAGGTCACTAATA +CCATCTAAGTAGTTGATTCATAGTGACTGCATATGTTGTGTTTTACAGTATTATGTAGTC +TGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGT +TCAGCTTTTTTATACTAAGTTGGCATTATAAAAAAGCATTGCTTATCAATTTGTTGCAAC +GAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGATTTCAATTTTGTCCCACTCCC +TGCCTCTGTCATCACGATACTGTGATGCCATGGTGTCCGACTTATGCCCGAGAAGATGTT +GAGCAAACTTATCGCTTATCTGCTTCTCATAGAGTCTTGCAGACAAACTGCGCAACTCGT +GAAAGGTAGGCGGATCCCCTTCGAAGGAAAGACCTGATGCTTTTCGTGCGCGCATAAAAT +ACCTTGATACTGTGCCGGATGAAAGCGGTTCGCGACGAGTAGATGCAATTATGGTTTCTC +CGCCAAGAATCTCTTTGCATTTATCAAGTGTTTCCTTCATTGATATTCCGAGAGCATCAA +TATGCAATGCTGTTGGGATGGCAATTTTTACGCCTGTTTTGCTTTGCTCGACATAAAGAT +ATCCATCTACGATATCAGACCACTTCATTTCGCATAAATCACCAACTCGTTGCCCGGTAA +CAACAGCCAGTTCCATTGCAAGTCTGAGCCAACATGGTGATGATTCTGCTGCTTGATAAA +TTTTCAGGTATTCGTCAGCCGTAAGTCTTGATCTCCTTACCTCTGATTTTGCTGCGCGAG +TGGCAGCGACATGGTTTGTTGTTATATGGCCTTCAGCTATTGCCTCTCGGAATGCATCGC +TCAGTGTTGATCTGATTAACTTGGCTGACGCCGCCTTGCCCTCGTCTATGTATCCATTGA +GCATTGCCGCAATTTCTTTTGTGGTGATGTCTTCAAGTGGAGCATCAGGCAGACCCCTCC +TTATTGCTTTAATTTTGCTCATGTAATTTATGAGTGTCTTCTGCTTGATTCCTCTGCTGG +CCAGGATTTTTTCGTAGCGATCAAGCCATGAATGTAACGTAACGGAATTATCACTGTTGA +TTCTCGCTGTCAGAGGCTTGTGTTTGTGTCCTGAAAATAACTCAATGTTGGCCTGTATAG +CTTCAGTGATTGCGATTCGCCTGTCTCTGCCTAATCCAAACTCTTTACCCGTCCTTGGGT +CCCTGTAGCAGTAATATCCATTGTTTCTTATATAAAGGTTAGGGGGTAAATCCCGGCGCT +CATGACTTCGCCTTCTTCCCATTTCTGATCCTCTTCAAAAGGCCACCTGTTACTGGTCGA +TTTAAGTCAACCTTTACCGCTGATTCGTGGAACAGATACTCTCTTCCATCCTTAACCGGA +GGTGGGAATATCCTGCATTCCCGAACCCATCGACGAACTGTTTCAAGGCTTCTTGGACGT +CGCTGGCGTGCGTTCCACTCCTGAAGTGTCAAGTACATCGCAAAGTCTCCGCAATTACAC +GCAAGAAAAAACCGCCATCAGGCGGCTTGGTGTTCTTTCAGTTCTTCAATTCGAATATTG +GTTACGTCTGCATGTGCTATCTGCGCCCATATCATCCAGTGGTCGTAGCAGTCGTTGATG +TTCTCCGCTTCGATAACTCTGTTGAATGGCTCTCCATTCCATTCTCCTGTGACTCGGAAG +TGCATTTATCATCTCCATAAAACAAAACCCGCCGTAGCGAGTTCAGATAAAATAAATCCC +CGCGAGTGCGAGGATTGTTATGTAATATTGGGTTTAATCATCTATATGTTTTGTACAGAG +AGGGCAAGTATCGTTTCCACCGTACTCGTGATAATAATTTTGCACGGTATCAGTCATTTC +TCGCACATTGCAGAATGGGGATTTGTCTTCATTAGACTTATAAACCTTCATGGAATATTT +GTATGCCGACTCTATATCTATACCTTCATCTACATAAACACCTTCGTGATGTCTGCATGG +AGACAAGACACCGGATCTGCACAACATTGATAACGCCCAATCTTTTTGCTCAGACTCTAA +CTCATTGATACTCATTTATAAACTCCTTGCAATGTATGTCGTTTCAGCTAAACGGTATCA +GCAATGTTTATGTAAAGAAACAGTAAGATAATACTCAACCCGATGTTTGAGTACGGTCAT +CATCTGACACTACAGACTCTGGCATCGCTGTGAAGACGACGCGAAATTCAGCATTTTCAC +AAGCGTTATCTTTTACAAAACCGATCTCACTCTCCTTTGATGCGAATGCCAGCGTCAGAC +ATCATATGCAGATACTCACCTGCATCCTGAACCCATTGACCTCCAACCCCGTAATAGCGA +TGCGTAATGATGTCGATAGTTACTAACGGGTCTTGTTCGATTAACTGCCGCAGAAACTCT +TCCAGGTCACCAGTGCAGTGCTTGATAACAGGAGTCTTCCCAGGATGGCGAACAACAAGA +AACTGGTTTCCGTCTTCACGGACTTCGTTGCTTTCCAGTTTAGCAATACGCTTACTCCCA +TCCGAGATAACACCTTCGTAATACTCACGCTGCTCGTTGAGTTTTGATTTTGCTGTTTCA +AGCTCAACACGCAGTTTCCCTACTGTTAGCGCAATATCCTCGTTCTCCTGGTCGCGGCGT +TTGATGTATTGCTGGTTTCTTTCCCGTTCATCCAGCAGTTCCAGCACAATCGATGGTGTT +ACCAATTCATGGAAAAGGTCTGCGTCAAATCCCCAGTCGTCATGCATTGCCTGCTCTGCC +GCTTCACGCAGTGCCTGAGAGTTAATTTCGCTCACTTCGAACCTCTCTGTTTACTGATAA +GTTCCAGATCCTCCTGGCAACTTGCACAAGTCCGACAACCCTGAACGACCAGGCGTCTTC +GTTCATCTATCGGATCGCCACACTCACAACAATGAGTGGCAGATATAGCCTGGTGGTTCA +GGCGGCGCATTTTTATTGCTGTGTTGCGCTGTAATTCTTCTATTTCTGATGCTGAATCAA +TGATGTCTGCCATCTTTCATTAATCCCTGAACTGTTGGTTAATACGCTTGAGGGTGAATG +CGAATAATAAAAAAGGAGCCTGTAGCTCCCTGATGATTTTGCTTTTCATGTTCATCGTTC +CTTAAAGACGCCGTTTAACATGCCGATTGCCAGGCTTAAATGAGTCGGTGTGAATCCCAT +CAGCGTTACCGTTTCGCGGTGCTTCTTCAGTACGCTACGGCAAATGTCATCGACGTTTTT +ATCCGGAAACTGCTGTCTGGCTTTTTTTGATTTCAGAATTAGCCTGACGGGCAATGCTGC +GAAGGGCGTTTTCCTGCTGAGGTGTCATTGAACAAGTCCCATGTCGGCAAGCATAAGCAC +ACAGAATATGAAGCCCGCTGCCAGAAAAATGCATTCCGTGGTTGTCATACCTGGTTTCTC +TCATCTGCTTCTGCTTTCGCCACCATCATTTCCAGCTTTTGTGAAAGGGATGCGGCTAAC +GTATGAAATTCTTCGTCTGTTTCTACTGGTATTGGCACAAACCTGATTCCAATTTGAGCA +AGGCTATGTGCCATCTCGATACTCGTTCTTAACTCAACAGAAGATGCTTTGTGCATACAG +CCCCTCGTTTATTATTTATCTCCTCAGCCAGCCGCTGTGCTTTCAGTGGATTTCGGATAA +CAGAAAGGCCGGGAAATACCCAGCCTCGCTTTGTAACGGAGTAGACGAAAGTGATTGCGC +CTACCCGGATATTATCGTGAGGATGCGTCATCGCCATTGCTCCCCAAATACAAAACCAAT +TTCAGCCAGTGCCTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAACTCGC +CATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGG +GTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTG +GGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATCCCG +GGAGGTAAACGGGCATTTCAGTTCAAGGCCGTTGCCGTCACTGCATAAACCATCGGGAGA +GCAGGCGGTACGCATACTTTCGTCGCGATAGATGATCGGGGATTCAGTAACATTCACGCC +GGAAGTGAATTCAAACAGGGTTCTGGCGTCGTTCTCGTACTGTTTTCCCCAGGCCAGTGC +TTTAGCGTTAACTTCCGGAGCCACACCGGTGCAAACCTCAGCAAGCAGGGTGTGGAAGTA +GGACATTTTCATGTCAGGCCACTTCTTTCCGGAGCGGGGTTTTGCTATCACGTTGTGAAC +TTCTGAAGCGGTGATGACGCCGAGCCGTAATTTGTGCCACGCATCATCCCCCTGTTCGAC +AGCTCTCACATCGATCCCGGTACGCTGCAGGATAATGTCCGGTGTCATGCTGCCACCTTC +TGCTCTGCGGCTTTCTGTTTCAGGAATCCAAGAGCTTTTACTGCTTCGGCCTGTGTCAGT +TCTGACGATGCACGAATGTCGCGGCGAAATATCTGGGAACAGAGCGGCAATAAGTCGTCA +TCCCATGTTTTATCCAGGGCGATCAGCAGAGTGTTAATCTCCTGCATGGTTTCATCGTTA +ACCGGAGTGATGTCGCGTTCCGGCTGACGTTCTGCAGTGTATGCAGTATTTTCGACAATG +CGCTCGGCTTCATCCTTGTCATAGATACCAGCAAATCCGAAGGCCAGACGGGCACACTGA +ATCATGGCTTTATGACGTAACATCCGTTTGGGATGCGACTGCCACGGCCCCGTGATTTCT +CTGCCTTCGCGAGTTTTGAATGGTTCGCGGCGGCATTCATCCATCCATTCGGTAACGCAG +ATCGGATGATTACGGTCCTTGCGGTAAATCCGGCATGTACAGGATTCATTGTCCTGCTCA +AAGTCCATGCCATCAAACTGCTGGTTTTCATTGATGATGCGGGACCAGCCATCAACGCCC +ACCACCGGAACGATGCCATTCTGCTTATCAGGAAAGGCGTAAATTTCTTTCGTCCACGGA +TTAAGGCCGTACTGGTTGGCAACGATCAGTAATGCGATGAACTGCGCATCGCTGGCATCA +CCTTTAAATGCCGTCTGGCGAAGAGTGGTGATCAGTTCCTGTGGGTCGACAGAATCCATG +CCGACACGTTCAGCCAGCTTCCCAGCCAGCGTTGCGAGTGCAGTACTCATTCGTTTTATA +CCTCTGAATCAATATCAACCTGGTGGTGAGCAATGGTTTCAACCATGTACCGGATGTGTT +CTGCCATGCGCTCCTGAAACTCAACATCGTCATCAAACGCACGGGTAATGGATTTTTTGC +TGGCCCCGTGGCGTTGCAAATGATCGATGCATAGCGATTCAAACAGGTGCTGGGGCAGGC +CTTTTTCCATGTCGTCTGCCAGTTCTGCCTCTTTCTCTTCACGGGCGAGCTGCTGGTAGT +GACGCGCCCAGCTCTGAGCCTCAAGACGATCCTGAATGTAATAAGCGTTCATGGCTGAAC +TCCTGAAATAGCTGTGAAAATATCGCCCGCGAAATGCCGGGCTGATTAGGAAAACAGGAA +AGGGGGTTAGTGAATGCTTTTGCTTGATCTCAGTTTCAGTATTAATATCCATTTTTTATA +AGCGTCGACGGCTTCACGAAACATCTTTTCATCGCCAATAAAAGTGGCGATAGTGAATTT +AGTCTGGATAGCCATAAGTGTTTGATCCATTCTTTGGGACTCCTGGCTGATTAAGTATGT +CGATAAGGCGTTTCCATCCGTCACGTAATTTACGGGTGATTCGTTCAAGTAAAGATTCGG +AAGGGCAGCCAGCAACAGGCCACCCTGCAATGGCATATTGCATGGTGTGCTCCTTATTTA +TACATAACGAAAAACGCCTCGAGTGAAGCGTTATTGGTATGCGGTAAAACCGCACTCAGG +CGGCCTTGATAGTCATATCATCTGAATCAAATATTCCTGATGTATCGATATCGGTAATTC +TTATTCCTTCGCTACCATCCATTGGAGGCCATCCTTCCTGACCATTTCCATCATTCCAGT +CGAACTCACACACAACACCATATGCATTTAAGTCGCTTGAAATTGCTATAAGCAGAGCAT +GTTGCGCCAGCATGATTAATACAGCATTTAATACAGAGCCGTGTTTATTGAGTCGGTATT +CAGAGTCTGACCAGAAATTATTAATCTGGTGAAGTTTTTCCTCTGTCATTACGTCATGGT +CGATTTCAATTTCTATTGATGCTTTCCAGTCGTAATCAATGATGTATTTTTTGATGTTTG +ACATCTGTTCATATCCTCACAGATAAAAAATCGCCCTCACACTGGAGGGCAAAGAAGATT +TCCAATAATCAGAACAAGTCGGCTCCTGTTTAGTTACGAGCGACATTGCTCCGTGTATTC +ACTCGTTGGAATGAATACACAGTGCAGTGTTTATTCTGTTATTTATGCCAAAAATAAAGG +CCACTATCAGGCAGCTTTGTTGTTCTGTTTACCAAGTTCTCTGGCAATCATTGCCGTCGT +TCGTATTGCCCATTTATCGACATATTTCCCATCTTCCATTACAGGAAACATTTCTTCAGG +CTTAACCATGCATTCCGATTGCAGCTTGCATCCATTGCATCGCTTGAATTGTCCACACCA +TTGATTTTTATCAATAGTCGTAGTCATACGGATAGTCCTGGTATTGTTCCATCACATCCT +GAGGATGCTCTTCGAACTCTTCAAATTCTTCTTCCATATATCACCTTAAATAGTGGATTG +CGGTAGTAAAGATTGTGCCTGTCTTTTAACCACATCAGGCTCGGTGGTTCTCGTGTACCC +CTACAGCGAGAAATCGGATAAACTATTACAACCCCTACAGTTTGATGAGTATAGAAATGG +ATCCACTCGTTATTCTCGGACGAGTGTTCAGTAATGAACCTCTGGAGAGAACCATGTATA +TGATCGTTATCTGGGTTGGACTTCTGCTTTTAAGCCCAGATAACTGGCCTGAATATGTTA +ATGAGAGAATCGGTATTCCTCATGTGTGGCATGTTTTCGTCTTTGCTCTTGCATTTTCGC +TAGCAATTAATGTGCATCGATTATCAGCTATTGCCAGCGCCAGATATAAGCGATTTAAGC +TAAGAAAACGCATTAAGATGCAAAACGATAAAGTGCGATCAGTAATTCAAAACCTTACAG +AAGAGCAATCTATGGTTTTGTGCGCAGCCCTTAATGAAGGCAGGAAGTATGTGGTTACAT +CAAAACAATTCCCATACATTAGTGAGTTGATTGAGCTTGGTGTGTTGAACAAAACTTTTT +CCCGATGGAATGGAAAGCATATATTATTCCCTATTGAGGATATTTACTGGACTGAATTAG +TTGCCAGCTATGATCCATATAATATTGAGATAAAGCCAAGGCCAATATCTAAGTAACTAG +ATAAGAGGAATCGATTTTCCCTTAATTTTCTGGCGTCCACTGCATGTTATGCCGCGTTCG +CCAGGCTTGCTGTACCATGTGCGCTGATTCTTGCGCTCAATACGTTGCAGGTTGCTTTCA +ATCTGTTTGTGGTATTCAGCCAGCACTGTAAGGTCTATCGGATTTAGTGCGCTTTCTACT +CGTGATTTCGGTTTGCGATTCAGCGAGAGAATAGGGCGGTTAACTGGTTTTGCGCTTACC +CCAACCAACAGGGGATTTGCTGCTTTCCATTGAGCCTGTTTCTCTGCGCGACGTTCGCGG +CGGCGTGTTTGTGCATCCATCTGGATTCTCCTGTCAGTTAGCTTTGGTGGTGTGTGGCAG +TTGTAGTCCTGAACGAAAACCCCCCGCGATTGGCACATTGGCAGCTAATCCGGAATCGCA +CTTACGGCCAATGCTTCGTTTCGTATCACACACCCCAAAGCCTTCTGCTTTGAATGCTGC +CCTTCTTCAGGGCTTAATTTTTAAGAGCGTCACCTTCATGGTGGTCAGTGCGTCCTGCTG +ATGTGCTCAGTATCACCGCCAGTGGTATTTATGTCAACACCGCCAGAGATAATTTATCAC +CGCAGATGGTTATCTGTATGTTTTTTATATGAATTTATTTTTTGCAGGGGGGCATTGTTT +GGTAGGTGAGAGATCTGAATTGCTATGTTTAGTGAGTTGTATCTATTTATTTTTCAATAA +ATACAATTGGTTATGTGTTTTGGGGGCGATCGTGAGGCAAAGAAAACCCGGCGCTGAGGC +CGGGTTATTCTTGTTCTCTGGTCAAATTATATAGTTGGAAAACAAGGATGCATATATGAA +TGAACGATGCAGAGGCAATGCCGATGGCGATAGTGGGTATCATGTAGCCGCTTATGCTGG +AAAGAAGCAATAACCCGCAGAAAAACAAAGCTCCAAGCTCAACAAAACTAAGGGCATAGA +CAATAACTACCGATGTCATATACCCATACTCTCTAATCTTGGCCAGTCGGCGCGTTCTGC +TTCCGATTAGAAACGTCAAGGCAGCAATCAGGATTGCAATCATGGTTCCTGCATATGATG +ACAATGTCGCCCCAAGACCATCTCTATGAGCTGAAAAAGAAACACCAGGAATGTAGTGGC +GGAAAAGGAGATAGCAAATGCTTACGATAACGTAAGGAATTATTACTATGTAAACACCAG +GCATGATTCTGTTCCGCATAATTACTCCTGATAATTAATCCTTAACTTTGCCCACCTGCC +TTTTAAAACATTCCAGTATATCACTTTTCATTCTTGCGTAGCAATATGCCATCTCTTCAG +CTATCTCAGCATTGGTGACCTTGTTCAGAGGCGCTGAGAGATGGCCTTTTTCTGATAGAT +AATGTTCTGTTAAAATATCTCCGGCCTCATCTTTTGCCCGCAGGCTAATGTCTGAAAATT +GAGGTGACGGGTTAAAAATAATATCCTTGGCAACCTTTTTTATATCCCTTTTAAATTTTG +GCTTAATGACTATATCCAATGAGTCAAAAAGCTCCCCTTCAATATCTGTTGCCCCTAAGA +CCTTTAATATATCGCCAAATACAGGTAGCTTGGCTTCTACCTTCACCGTTGTTCGGCCGA +TGAAATGCATATGCATAACATCGTCTTTGGTGGTTCCCCTCATCAGTGGCTCTATCTGAA +CGCGCTCTCCACTGCTTAATGACATTCCTTTCCCGATTAAAAAATCTGTCAGATCGGATG +TGGTCGGCCCGAAAACAGTTCTGGCAAAACCAATGGTGTCGCCTTCAACAAACAAAAAAG +ATGGGAATCCCAATGATTCGTCATCTGCGAGGCTGTTCTTAATATCTTCAACTGAAGCTT +TAGAGCGATTTATCTTCTGAACCAGACTCTTGTCATTTGTTTTGGTAAAGAGAAAAGTTT +TTCCATCGATTTTATGAATATACAAATAATTGGAGCCAACCTGCAGGTGATGATTATCAG +CCAGCAGAGAATTAAGGAAAACAGACAGGTTTATTGAGCGCTTATCTTTCCCTTTATTTT +TGCTGCGGTAAGTCGCATAAAAACCATTCTTCATAATTCAATCCATTTACTATGTTATGT +TCTGAGGGGAGTGAAAATTCCCCTAATTCGATGAAGATTCTTGCTCAATTGTTATCAGCT +ATGCGCCGACCAGAACACCTTGCCGATCAGCCAAACGTCTCTTCAGGCCACTGACTAGCG +ATAACTTTCCCCACAACGGAACAACTCTCATTGCATGGGATCATTGGGTACTGTGGGTTT +AGTGGTTGTAAAAACACCTGACCGCTATCCCTGATCAGTTTCTTGAAGGTAAACTCATCA +CCCCCAAGTCTGGCTATGCAGAAATCACCTGGCTCAACAGCCTGCTCAGGGTCAACGAGA +ATTAACATTCCGTCAGGAAAGCTTGGCTTGGAGCCTGTTGGTGCGGTCATGGAATTACCT +TCAACCTCAAGCCAGAATGCAGAATCACTGGCTTTTTTGGTTGTGCTTACCCATCTCTCC +GCATCACCTTTGGTAAAGGTTCTAAGCTTAGGTGAGAACATCCCTGCCTGAACATGAGAA +AAAACAGGGTACTCATACTCACTTCTAAGTGACGGCTGCATACTAACCGCTTCATACATC +TCGTAGATTTCTCTGGCGATTGAAGGGCTAAATTCTTCAACGCTAACTTTGAGAATTTTT +GTAAGCAATGCGGCGTTATAAGCATTTAATGCATTGATGCCATTAAATAAAGCACCAACG +CCTGACTGCCCCATCCCCATCTTGTCTGCGACAGATTCCTGGGATAAGCCAAGTTCATTT +TTCTTTTTTTCATAAATTGCTTTAAGGCGACGTGCGTCCTCAAGCTGCTCTTGTGTTAAT +GGTTTCTTTTTTGTGCTCATACGTTAAATCTATCACCGCAAGGGATAAATATCTAACACC +GTGCGTGTTGACTATTTTACCTCTGGCGGTGATAATGGTTGCATGTACTAAGGAGGTTGT +ATGGAACAACGCATAACCCTGAAAGATTATGCAATGCGCTTTGGGCAAACCAAGACAGCT +AAAGATCTCGGCGTATATCAAAGCGCGATCAACAAGGCCATTCATGCAGGCCGAAAGATT +TTTTTAACTATAAACGCTGATGGAAGCGTTTATGCGGAAGAGGTAAAGCCCTTCCCGAGT +AACAAAAAAACAACAGCATAAATAACCCCGCTCTTACACATTCCAGCCCTGAAAAAGGGC +ATCAAATTAAACCACACCTATGGTGTATGCATTTATTTGCATACATTCAATCAATTGTTA +TCTAAGGAAATACTTACATATGGTTCGTGCAAACAAACGCAACGAGGCTCTACGAATCGA +GAGTGCGTTGCTTAACAAAATCGCAATGCTTGGAACTGAGAAGACAGCGGAAGCTGTGGG +CGTTGATAAGTCGCAGATCAGCAGGTGGAAGAGGGACTGGATTCCAAAGTTCTCAATGCT +GCTTGCTGTTCTTGAATGGGGGGTCGTTGACGACGACATGGCTCGATTGGCGCGACAAGT +TGCTGCGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCA +GATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCATTATGACAAATACAGCA +AAAATACTCAACTTCGGCAGAGGTAACTTTGCCGGACAGGAGCGTAATGTGGCAGATCTC +GATGATGGTTACGCCAGACTATCAAATATGCTGCTTGAGGCTTATTCGGGCGCAGATCTG +ACCAAGCGACAGTTTAAAGTGCTGCTTGCCATTCTGCGTAAAACCTATGGGTGGAATAAA +CCAATGGACAGAATCACCGATTCTCAACTTAGCGAGATTACAAAGTTACCTGTCAAACGG +TGCAATGAAGCCAAGTTAGAACTCGTCAGAATGAATATTATCAAGCAGCAAGGCGGCATG +TTTGGACCAAATAAAAACATCTCAGAATGGTGCATCCCTCAAAACGAGGGAAAATCCCCT +AAAACGAGGGATAAAACATCCCTCAAATTGGGGGATTGCTATCCCTCAAAACAGGGGGAC +ACAAAAGACACTATTACAAAAGAAAAAAGAAAAGATTATTCGTCAGAGAATTCTGGCGAA +TCCTCTGACCAGCCAGAAAACGACCTTTCTGTGGTGAAACCGGATGCTGCAATTCAGAGC +GGCAGCAAGTGGGGGACAGCAGAAGACCTGACCGCCGCAGAGTGGATGTTTGACATGGTG +AAGACTATCGCACCATCAGCCAGAAAACCGAATTTTGCTGGGTGGGCTAACGATATCCGC +CTGATGCGTGAACGTGACGGACGTAACCACCGCGACATGTGTGTGCTGTTCCGCTGGGCA +TGCCAGGACAACTTCTGGTCCGGTAACGTGCTGAGCCCGGCCAAACTCCGCGATAAGTGG +ACCCAACTCGAAATCAACCGTAACAAGCAACAGGCAGGCGTGACAGCCAGCAAACCAAAA +CTCGACCTGACAAACACAGACTGGATTTACGGGGTGGATCTATGAAAAACATCGCCGCAC +AGATGGTTAACTTTGACCGTGAGCAGATGCGTCGGATCGCCAACAACATGCCGGAACAGT +ACGACGAAAAGCCGCAGGTACAGCAGGTAGCGCAGATCATCAACGGTGTGTTCAGCCAGT +TACTGGCAACTTTCCCGGCGAGCCTGGCTAACCGTGACCAGAACGAAGTGAACGAAATCC +GTCGCCAGTGGGTTCTGGCTTTTCGGGAAAACGGGATCACCACGATGGAACAGGTTAACG +CAGGAATGCGCGTAGCCCGTCGGCAGAATCGACCATTTCTGCCATCACCCGGGCAGTTTG +TTGCATGGTGCCGGGAAGAAGCATCCGTTACCGCCGGACTGCCAAACGTCAGCGAGCTGG +TTGATATGGTTTACGAGTATTGCCGGAAGCGAGGCCTGTATCCGGATGCGGAGTCTTATC +CGTGGAAATCAAACGCGCACTACTGGCTGGTTACCAACCTGTATCAGAACATGCGGGCCA +ATGCGCTTACTGATGCGGAATTACGCCGTAAGGCCGCAGATGAGCTTGTCCATATGACTG +CGAGAATTAACCGTGGTGAGGCGATCCCTGAACCAGTAAAACAACTTCCTGTCATGGGCG +GTAGACCTCTAAATCGTGCACAGGCTCTGGCGAAGATCGCAGAAATCAAAGCTAAGTTCG +GACTGAAAGGAGCAAGTGTATGACGGGCAAAGAGGCAATTATTCATTACCTGGGGACGCA +TAATAGCTTCTGTGCGCCGGACGTTGCCGCGCTAACAGGCGCAACAGTAACCAGCATAAA +TCAGGCCGCGGCTAAAATGGCACGGGCAGGTCTTCTGGTTATCGAAGGTAAGGTCTGGCG +AACGGTGTATTACCGGTTTGCTACCAGGGAAGAACGGGAAGGAAAGATGAGCACGAACCT +GGTTTTTAAGGAGTGTCGCCAGAGTGCCGCGATGAAACGGGTATTGGCGGTATATGGAGT +TAAAAGATGACCATCTACATTACTGAGCTAATAACAGGCCTGCTGGTAATCGCAGGCCTT +TTTATTTGGGGGAGAGGGAAGTCATGAAAAAACTAACCTTTGAAATTCGATCTCCAGCAC +ATCAGCAAAACGCTATTCACGCAGTACAGCAAATCCTTCCAGACCCAACCAAACCAATCG +TAGTAACCATTCAGGAACGCAACCGCAGCTTAGACCAAAACAGGAAGCTATGGGCCTGCT +TAGGTGACGTCTCTCGTCAGGTTGAATGGCATGGTCGCTGGCTGGATGCAGAAAGCTGGA +AGTGTGTGTTTACCGCAGCATTAAAGCAGCAGGATGTTGTTCCTAACCTTGCCGGGAATG +GCTTTGTGGTAATAGGCCAGTCAACCAGCAGGATGCGTGTAGGCGAATTTGCGGAGCTAT +TAGAGCTTATACAGGCATTCGGTACAGAGCGTGGCGTTAAGTGGTCAGACGAAGCGAGAC +TGGCTCTGGAGTGGAAAGCGAGATGGGGAGACAGGGCTGCATGATAAATGTCGTTAGTTT +CTCCGGTGGCAGGACGTCAGCATATTTGCTCTGGCTAATGGAGCAAAAGCGACGGGCAGG +TAAAGACGTGCATTACGTTTTCATGGATACAGGTTGTGAACATCCAATGACATATCGGTT +TGTCAGGGAAGTTGTGAAGTTCTGGGATATACCGCTCACCGTATTGCAGGTTGATATCAA +CCCGGAGCTTGGACAGCCAAATGGTTATACGGTATGGGAACCAAAGGATATTCAGACGCG +AATGCCTGTTCTGAAGCCATTTATCGATATGGTAAAGAAATATGGCACTCCATACGTCGG +CGGCGCGTTCTGCACTGACAGATTAAAACTCGTTCCCTTCACCAAATACTGTGATGACCA +TTTCGGGCGAGGGAATTACACCACGTGGATTGGCATCAGAGCTGATGAACCGAAGCGGCT +AAAGCCAAAGCCTGGAATCAGATATCTTGCTGAACTGTCAGACTTTGAGAAGGAAGATAT +CCTCGCATGGTGGAAGCAACAACCATTCGATTTGCAAATACCGGAACATCTCGGTAACTG +CATATTCTGCATTAAAAAATCAACGCAAAAAATCGGACTTGCCTGCAAAGATGAGGAGGG +ATTGCAGCGTGTTTTTAATGAGGTCATCACGGGATCCCATGTGCGTGACGGACATCGGGA +AACGCCAAAGGAGATTATGTACCGAGGAAGAATGTCGCTGGACGGTATCGCGAAAATGTA +TTCAGAAAATGATTATCAAGCCCTGTATCAGGACATGGTACGAGCTAAAAGATTCGATAC +CGGCTCTTGTTCTGAGTCATGCGAAATATTTGGAGGGCAGCTTGATTTCGACTTCGGGAG +GGAAGCTGCATGATGCGATGTTATCGGTGCGGTGAATGCAAAGAAGATAACCGCTTCCGA +CCAAATCAACCTTACTGGAATCGATGGTGTCTCCGGTGTGAAAGAACACCAACAGGGGTG +TTACCACTACCGCAGGAAAAGGAGGACGTGTGGCGAGACAGCGACGAAGTATCACCGACA +TAATCTGCGAAAACTGCAAATACCTTCCAACGAAACGCACCAGAAATAAACCCAAGCCAA +TCCCAAAAGAATCTGACGTAAAAACCTTCAACTACACGGCTCACCTGTGGGATATCCGGT +GGCTAAGACGTCGTGCGAGGAAAACAAGGTGATTGACCAAAATCGAAGTTACGAACAAGA +AAGCGTCGAGCGAGCTTTAACGTGCGCTAACTGCGGTCAGAAGCTGCATGTGCTGGAAGT +TCACGTGTGTGAGCACTGCTGCGCAGAACTGATGAGCGATCCGAATAGCTCGATGCACGA +GGAAGAAGATGATGGCTAAACCAGCGCGAAGACGATGTAAAAACGATGAATGCCGGGAAT +GGTTTCACCCTGCATTCGCTAATCAGTGGTGGTGCTCTCCAGAGTGTGGAACCAAGATAG +CACTCGAACGACGAAGTAAAGAACGCGAAAAAGCGGAAAAAGCAGCAGAGAAGAAACGAC +GACGAGAGGAGCAGAAACAGAAAGATAAACTTAAGATTCGAAAACTCGCCTTAAAGCCCC +GCAGTTACTGGATTAAACAAGCCCAACAAGCCGTAAACGCCTTCATCAGAGAAAGAGACC +GCGACTTACCATGTATCTCGTGCGGAACGCTCACGTCTGCTCAGTGGGATGCCGGACATT +ACCGGACAACTGCTGCGGCACCTCAACTCCGATTTAATGAACGCAATATTCACAAGCAAT +GCGTGGTGTGCAACCAGCACAAAAGCGGAAATCTCGTTCCGTATCGCGTCGAACTGATTA +GCCGCATCGGGCAGGAAGCAGTAGACGAAATCGAATCAAACCATAACCGCCATCGCTGGA +CTATCGAAGAGTGCAAGGCGATCAAGGCAGAGTACCAACAGAAACTCAAAGACCTGCGAA +ATAGCAGAAGTGAGGCCGCATGACGTTCTCAGTAAAAACCATTCCAGACATGCTCGTTGA +AACATACGGAAATCAGACAGAAGTAGCACGCAGACTGAAATGTAGTCGCGGTACGGTCAG +AAAATACGTTGATGATAAAGACGGGAAAATGCACGCCATCGTCAACGACGTTCTCATGGT +TCATCGCGGATGGAGTGAAAGAGATGCGCTATTACGAAAAAATTGATGGCAGCAAATACC +GAAATATTTGGGTAGTTGGCGATCTGCACGGATGCTACACGAACCTGATGAACAAACTGG +ATACGATTGGATTCGACAACAAAAAAGACCTGCTTATCTCGGTGGGCGATTTGGTTGATC +GTGGTGCAGAGAACGTTGAATGCCTGGAATTAATCACATTCCCCTGGTTCAGAGCTGTAC +GTGGAAACCATGAGCAAATGATGATTGATGGCTTATCAGAGCGTGGAAACGTTAATCACT +GGCTGCTTAATGGCGGTGGCTGGTTCTTTAATCTCGATTACGACAAAGAAATTCTGGCTA +AAGCTCTTGCCCATAAAGCAGATGAACTTCCGTTAATCATCGAACTGGTGAGCAAAGATA +AAAAATATGTTATCTGCCACGCCGATTATCCCTTTGACGAATACGAGTTTGGAAAGCCAG +TTGATCATCAGCAGGTAATCTGGAACCGCGAACGAATCAGCAACTCACAAAACGGGATCG +TGAAAGAAATCAAAGGCGCGGACACGTTCATCTTTGGTCATACGCCAGCAGTGAAACCAC +TCAAGTTTGCCAACCAAATGTATATCGATACCGGCGCAGTGTTCTGCGGAAACCTAACAT +TGATTCAGGTACAGGGAGAAGGCGCATGAGACTCGAAAGCGTAGCTAAATTTCATTCGCC +AAAAAGCCCGATGATGAGCGACTCACCACGGGCCACGGCTTCTGACTCTCTTTCCGGTAC +TGATGTGATGGCTGCTATGGGGATGGCGCAATCACAAGCCGGATTCGGTATGGCTGCATT +CTGCGGTAAGCACGAACTCAGCCAGAACGACAAACAAAAGGCTATCAACTATCTGATGCA +ATTTGCACACAAGGTATCGGGGAAATACCGTGGTGTGGCAAAGCTTGAAGGAAATACTAA +GGCAAAGGTACTGCAAGTGCTCGCAACATTCGCTTATGCGGATTATTGCCGTAGTGCCGC +GACGCCGGGGGCAAGATGCAGAGATTGCCATGGTACAGGCCGTGCGGTTGATATTGCCAA +AACAGAGCTGTGGGGGAGAGTTGTCGAGAAAGAGTGCGGAAGATGCAAAGGCGTCGGCTA +TTCAAGGATGCCAGCAAGCGCAGCATATCGCGCCGTGACGATGCTAATCCCAAACCTTAC +CCAACCCACCTGGTCACGCACTGTTAAGCCGCTGTATGACGCTCTGGTGGTGCAATGCCA +CAAAGAAGAGTCAATCGCAGACAACATTTTGAATGCGGTCACACGTTAGCAGCATGATTG +CCACGGATGGCAACATATTAACGGCATGATATTGACTTATTGAATAAAATTGGGTAAATT +TGACTCAACGATGGGTTAATTCGCTCGTTGTGGTAGTGAGATGAAAAGAGGCGGCGCTTA +CTACCGATTCCGCCTAGTTGGTCACTTCGACGTATCGTCTGGAACTCCAACCATCGCAGG +CAGAGAGGTCTGCAAAATGCAATCCCGAAACAGTTCGCAGGTAATAGTTAGAGCCTGCAT +AACGGTTTCGGGATTTTTTATATCTGCACAACAGGTAAGAGCATTGAGTCGATAATCGTG +AAGAGTCGGCGAGCCTGGTTAGCCAGTGCTCTTTCCGTTGTGCTGAATTAAGCGAATACC +GGAAGCAGAACCGGATCACCAAATGCGTACAGGCGTCATCGCCGCCCAGCAACAGCACAA +CCCAAACTGAGCCGTAGCCACTGTCTGTCCTGAATTCATTAGTAATAGTTACGCTGCGGC +CTTTTACACATGACCTTCGTGAAAGCGGGTGGCAGGAGGTCGCGCTAACAACCTCCTGCC +GTTTTGCCCGTGCATATCGGTCACGAACAAATCTGATTACTAAACACAGTAGCCTGGATT +TGTTCTATCAGTAATCGACCTTATTCCTAATTAAATAGAGCAAATCCCCTTATTGGGGGT +AAGACATGAAGATGCCAGAAAAACATGACCTGTTGGCCGCCATTCTCGCGGCAAAGGAAC +AAGGCATCGGGGCAATCCTTGCGTTTGCAATGGCGTACCTTCGCGGCAGATATAATGGCG +GTGCGTTTACAAAAACAGTAATCGACGCAACGATGTGCGCCATTATCGCCTAGTTCATTC +GTGACCTTCTCGACTTCGCCGGACTAAGTAGCAATCTCGCTTATATAACGAGCGTGTTTA +TCGGCTACATCGGTACTGACTCGATTGGTTCGCTTATCAAACGCTTCGCTGCTAAAAAAG +CCGGAGTAGAAGATGGTAGAAATCAATAATCAACGTAAGGCGTTCCTCGATATGCTGGCG +TGGTCGGAGGGAACTGATAACGGACGTCAGAAAACCAGAAATCATGGTTATGACGTCATT +GTAGGCGGAGAGCTATTTACTGATTACTCCGATCACCCTCGCAAACTTGTCACGCTAAAC +CCAAAACTCAAATCAACAGGCGCCGGACGCTACCAGCTTCTTTCCCGTTGGTGGGATGCC +TACCGCAAGCAGCTTGGCCTGAAAGACTTCTCTCCGAAAAGTCAGGACGCTGTGGCATTG +CAGCAGATTAAGGAGCGTGGCGCTTTACCTATGATTGATCGTGGTGATATCCGTCAGGCA +ATCGACCGTTGCAGCAATATCTGGGCTTCACTGCCGGGCGCTGGTTATGGTCAGTTCGAG +CATAAGGCTGACAGCCTGATTGCAAAATTCAAAGAAGCGGGCGGAACGGTCAGAGAGATT +GATGTATGAGCAGAGTCACCGCGATTATCTCCGCTCTGGTTATCTGCATCATCGTCTGCC +TGTCATGGGCTGTTAATCATTACCGTGATAACGCCATTACCTACAAAGCCCAGCGCGACA +AAAATGCCAGAGAACTGAAGCTGGCGAACGCGGCAATTACTGACATGCAGATGCGTCAGC +GTGATGTTGCTGCGCTCGATGCAAAATACACGAAGGAGTTAGCTGATGCTAAAGCTGAAA +ATGATGCTCTGCGTGATGATGTTGCCGCTGGTCGTCGTCGGTTGCACATCAAAGCAGTCT +GTCAGTCAGTGCGTGAAGCCACCACCGCCTCCGGCGTGGATAATGCAGCCTCCCCCCGAC +TGGCAGACACCGCTGAACGGGATTATTTCACCCTCAGAGAGAGGCTGATCACTATGCAAA +AACAACTGGAAGGAACCCAGAAGTATATTAATGAGCAGTGCAGATAGAGTTGCCCATATC +GATGGGCAACTCATGCAATTATTGTGAGCAATACACACGCGCTTCCAGCGGAGTATAAAT +GCCTAAAGTAATAAAACCGAGCAATCCATTTACGAATGTTTGCTGGGTTTCTGTTTTAAC +AACATTTTCTGCGCCGCCACAAATTTTGGCTGCATCGACAGTTTTCTTCTGCCCAATTCC +AGAAACGAAGAAATGATGGGTGATGGTTTCCTTTGGTGCTACTGCTGCCGGTTTGTTTTG +AACAGTAAACGTCTGTTGAGCACATCCTGTAATAAGCAGGGCCAGCGCAGTAGCGAGTAG +CATTTTTTTCATGGTGTTATTCCCGATGCTTTTTGAAGTTCGCAGAATCGTATGTGTAGA +AAATTAAACAAACCCTAAACAATGAGTTGAAATTTCATATTGTTAATATTTATTAATGTA +TGTCAGGTGCGATGAATCGTCATTGTATTCCCGGATTAACTATGTCCACAGCCCTGACGG +GGAACTTCTCTGCGGGAGTGTCCGGGAATAATTAAAACGATGCACACAGGGTTTAGCGCG +TACACGTATTGCATTATGCCAACGCCCCGGTGCTGACACGGAAGAAACCGGACGTTATGA +TTTAGCGTGGAAAGATTTGTGTAGTGTTCTGAATGCTCTCAGTAAATAGTAATGAATTAT +CAAAGGTATAGTAATATCTTTTATGTTCATGGATATTTGTAACCCATCGGAAAACTCCTG +CTTTAGCAAGATTTTCCCTGTATTGCTGAAATGTGATTTCTCTTGATTTCAACCTATCAT +AGGACGTTTCTATAAGATGCGTGTTTCTTGAGAATTTAACATTTACAACCTTTTTAAGTC +CTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGAT +AGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTA +AATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAA +CCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGT +CTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAAT +AGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGAC +AGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTT +AACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAATCTGCTGA +TGATCCCTCCGTGGATCTGATTCGTGTAAAAAATATGCTTAATAGCACCATTTCTATGAG +TTACCCTGATGTTGTAATTGCATGTATAGAACATAAGGTGTCTCTGGAAGCATTCAGAGC +AATTGAGGCAGCGTTGGTGAAGCACGATAATAATATGAAGGATTATTCCCTGGTGGTTGA +CTGATCACCATAACTGCTAATCATTCAAACTATTTAGTCTGTGACAGAGCCAACACGCAG +TCTGTCACTGTCAGGAAAGTGGTAAAACTGCAACTCAATTACTGCAATGCCCTCGTAATT +AAGTGAATTTACAATATCGTCCTGTTCGGAGGGAAGAACGCGGGATGTTCATTCTTCATC +ACTTTTAATTGATGTATATGCTCTCTTTTCTGACGTTAGTCTCCGACGGCAGGCTTCAAT +GACCCAGGCTGAGAAATTCCCGGACCCTTTTTGCTCAAGAGCGATGTTAATTTGTTCAAT +CATTTGGTTAGGAAAGCGGATGTTGCGGGTTGTTGTTCTGCGGGTTCTGTTCTTCGTTGA +CATGAGGTTGCCCCGTATTCAGTGTCGCTGATTTGTATTGTCTGAAGTTGTTTTTACGTT +AAGTTGATGCAGATCAATTAATACGATACCTGCGTCATAATTGATTATTTGACGTGGTTT +GATGGCCTCCACGCACGTTGTGATATGTAGATGATAATCATTATCACTTTACGGGTCCTT +TCCGGTGATCCGACAGGTTACG diff --git a/src/squirrel-1.4/seqs/lorist2.vep b/src/squirrel-1.4/seqs/lorist2.vep new file mode 100644 index 0000000..e19a34b --- /dev/null +++ b/src/squirrel-1.4/seqs/lorist2.vep @@ -0,0 +1,97 @@ + +CTATGTATTCTATAGTGTCACCTAAATCGTATGTGTATGATACATAAGGTTATGT +ATTAATTGTAGCCGCGTTCTAACGACAATATGTACAAGCCTAATTGTGTAGCATCTGGCT +TACTGAAGCAGACCCTATCATCTCTCTCGTAAACTGCCGTCAGAGTCGGTTTGGTTGGAC +GAACCTTCTGAGTTTCTGGTAACGCCGTCCCGCACCCGGAAATGGTCAGCGAACCAATCA +GCAGGGTCATCGCTAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAATTC +TAGCCAGATCCCCGGGCGGGCCTGATTTATGCTGGTTACTGTTGCGCCTGTTA +GCGCGGCAACGTCCGGCGCACAGAAGCTATTATGCGTCCCCAGGTAATGAATAATTGCCT +CTTTGCCCGTCATACACTTGCTCCTTTCAGTCCGAACTTAGCTTTGATTTCTGCGATCTT +CGCCAGAGCCTGTGCACGATTTAGAGGTCTACCGCCCATGACAGGAAGTTGTTTTACTGG +TTCAGGGATCGCCTCACCACGGTTAATTCTCGCAGTCATATGGACAAGCTCATCTGCGGC +CTTACGGCGTAATTCCGCATCAGTAAGCGCATTGGCCCGCATGTTCTGATACAGGTTGGT +AACCAGCCAGTAGTGCGCGTTTGATTTCCACGGATAAGACTCCGCATCCGGATACAGGCC +TCGCTTCCGGCAATACTCGTAAACCATATCAACCAGCTCGCTGACGTTTGGCAGTCCGGC +GGTAACGGATGCTTCTTCCCGGCACCATGCAACAAACTGCCCGGGTGATGGCAGAAATGG +TCGATTCTGCCGACGGGCTACGCGCATTCCTGCGTTAACCTGTTCCATCGTGGTGATCCC +GTTTTCCCGAAAAGCCAGAACCCACTGGCGACGGATTTCGTTCACTTCGTTCTGGTCACG +GTTAGCCAGGCTCGCCGGGAAAGTTGCCAGTAACTGGCTGAACACACCGTTGATGATCTG +CGCTACCTGCTGTACCTGCGGCTTTTCGTCGTACTGTTCCGGCATGTTGTTGGCGATCCG +ACGCATCTGCTCACGGTCAAAGTTAACCATCTGTGCGGCGATGTTTTTCATAGATCCACC +CCGTAAATCCAGTCTGTGTTTGTCAGGTCGAGTTTTGGTTTGCTGGCTGTCACGCCTGCC +TGTTGCTTGTTACGGTTGATTTCGAGTTGGGTCCACTTATCGCGGAGTTTGGCCGGGCTC +AGCACGTTACCGGACCAGAAGTTGTCCTGGCATGCCCAGCGGAACAGCACACACATGTCG +CGGTGGTTACGTCCGTCACGTTCACGCATCAGGCGGATATCGTTAGCCCACCCAGCAAAA +TTCGGTTTTCTGGCTGATGGTGCGATAGTCTTCACCATGTCAAACATCCACTCTGCGGCG +GTCAGGTCTTCTGCTGTCCCCCACTTGCTGCCGCTCTGAATTGCAGCATCCGGTTTCACC +ACAGAAAGGTCGTTTTCTGGCTGGTCAGAGGATTCGCCAGAATTCTCTGACGAATAATCT +TTTCTTTTTTCTTTTGTAATAGTGTCTTTTGTGTCCCCCTGTTTTGAGGGATAGCAATCC +CCCAATTTGAGGGATGTTTTATCCCTCGTTTTAGGGGATTTTCCCTCGTTTTGAGGGATG +CACCATTCTGAGATGTTTTTATTTGGTCCAAACATGCCGCCTTGCTGCTTGATAATATTC +ATTCTGACGAGTTCTAACTTGGCTTCATTGCACCGTTTGACAGGTAACTTTGTAATCTCG +CTAAGTTGAGAATCGGTGATTCTGTCCATTGGTTTATTCCACCCATAGGTTTTACGCAGA +ATGGCAAGCAGCACTTTAAACTGTCGCTTGGTCAGATCTGCGCCCGAATAAGCCTCAAGC +AGCATATTTGATAGTCTGGCGTAACCATCATCGAGATCTGCCACATTACGCTCCTGTCCG +GCAAAGTTACCTCTGCCGAAGTTGAGTATTTTTGCTGTATTTGTCATAATGACTCCTGTT +GATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCG +CCGGGCGTTTTTTATTGGTGAGAATCGCAGCAACTTGTCGCGCCAATCGAGCCATGTCGT +CGTCAACGACCCCCCATTCAAGAACAGCAAGCAGCATTGAGAACTTTGGAATCCAGTCCC +TCTTCCACCTGCTGATCTGCGACTTATCAACGCCCACAGCTTCCGCTGTCTTCTCAGTTC +CAAGCATTGCGATTTTGTTAAGCAACGCACTCTCGATTCGTAGAGCCTCGTTGCGTTTGT +TTGCACGAACCATATGTAAGTATTTCCTTAGATAACAATTGATTGAATGTATGCAAATAA +ATGCATACACCATAGGTGTGGTTTAATTTGATGCCCTTTTTCAGGGCTGGAATGTGTAAG +AGCGGGGTTATTTATGCTGTTGTTTTTTTGTTACTCGGGAAGGGCTTTACCTCTTCCGCA +TAAACGCTTCCATCAGCGTTTATAGTTAAAAAAATCTTTCGGCCTGCATGAATGGCCTTG +TTGATCGCGCTTTGATATACGCCGAGATCTTTAGCTGTCTTGGTTTGCCCAAAGCGCATT +GCATAATCTTTCAGGGTTATGCGTTGTTCCATACAACCTCCTTAGTACATGCAACCATTA +TCACCGCCAGAGGTAAAATAGTCAACACGCACGGTGTTAGATATTTATCCCTTGCGGTGA +TAGATTTAACGTATGAGCACAAAAAAGAAACCATTAACACAAGAGCAGCTTGAGGACGCA +CGTCGCCTTAAAGCAATTTATGAAAAAAAGAAAAATGAACTTGGCTTATCCCAGGAATCT +GTCGCAGACAAGATGGGGATGGGGCAGTCAGGCGTTGGTGCTTTATTTAATGGCATCAAT +GCATTAAATGCTTATAACGCCGCATTGCTTACAAAAATTCTCAAAGTTAGCGTTGAAGAA +TTTAGCCCTTCAATCGCCAGAGAAATCTACGAGATGTATGAAGCGGTTAGTATGCAGCCG +TCACTTAGAAGTGAGTATGAGTACCCTGTTTTTTCTCATGTTCAGGCAGGGATGTTCTCA +CCTAAGCT +AGCTTCACGCTGCCGCAAGCACTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGT +AGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCT +GGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGC +GATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGC +CCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGA +TCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGA +TTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCT +ATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGC +AGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGG +ACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCG +ACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATC +TCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGC +GGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCG +AGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGC +ATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCG +AGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCC +GCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAG +CGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCG +TGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACG +AGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCC +ATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTT +CCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCA +CCCCGGGCTCGATCCCCTCGCGAGTTGGTTCAGCTGCTGCCTGAGGCTGGACGACCTCGC +GGAGTTCTACCGGCAGTGCAAATCCGTCGGCATCCAGGAAACCAGCAGCGGCTATCCGCG +CATCCATGCCCCCGAACTGCAGGAGTGGGGAGGCACGATGGCCGCTTTGGTCGGATCAAT +TCGCGCGACGG +ATCGATCCGGGCAACGTTGTTGCCATTGCTGCAGGGGGGGGGGGGGGGGGGGGGGGGGGT +TGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAA +AAGCCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAAAAAC +ATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCG +AAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTG +ATAATGATTATCATCTACATATCACAACGTGCGTGGAGGCCATCAAACCACGTCAAATAA +TCAATTATGACGCAGGTATCGTATTAATTGATCTGCATCAACTTAACGTAAAAACAACTT +CAGACAATACAAATCAGCGACACTGAATACGGGGCAACCTCATGTCAACCCCCCCCCCCCCCC +CCCCCCCCCCCTGCAGGCGGAGAACTGGTAGGTATGGAAGATCTCTAGAGAATTCAAAAA +AAGCCCGCTCATTAGGCGGGCTAATTCGAGCT +CGCCCGGGGATCGATCCTCTAGAGTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCG +GTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAA +GCCAGTATATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCG +CCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAA +GCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGC +GCGAGGCCCAGCTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCGGAAGCTTA +GGATC diff --git a/src/squirrel-1.4/seqs/lorist6.vep b/src/squirrel-1.4/seqs/lorist6.vep new file mode 100644 index 0000000..a94a432 --- /dev/null +++ b/src/squirrel-1.4/seqs/lorist6.vep @@ -0,0 +1,109 @@ +GATCCTAGTATTCTATAGTGTCACCTAAATCGTATGTGTATGATACATAA +GGTTATGTATTAATTGTAGCCGCGTTCTAACGACAATATGTACAAGCCTA +ATTGTGTAGCATCTGGCTTACTGAAGCAGACCCTATCATCTCTCTCGTAA +ACTGCCGTCAGAGTCGGTTTGGTTGGACGAACCTTCTGAGTTTCTGGTAA +CGCCGTCCCGCACCCGGAAATGGTCAGCGAACCAATCAGCAGGGTCATCG +CTAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAATTCTA +GCCAGATCCCCGCTGATTTATGCTGGTTACTGTTGCGCCTGTTAGCGCGG +CAACGTCCGGCGCACAGAAGCTATTATGCGTCCCCAGGTAATGAATAATT +GCCTCTTTGCCCGTCATACACTTGCTCCTTTCAGTCCGAACTTAGCTTTG +ATTTCTGCGATCTTCGCCAGAGCCTGTGCACGATTTAGAGGTCTACCGCC +CATGACAGGAAGTTGTTTTACTGGTTCAGGGATCGCCTCACCACGGTTAA +TTCTCGCAGTCATATGGACAAGCTCATCTGCGGCCTTACGGCGTAATTCC +GCATCAGTAAGCGCATTGGCCCGCATGTTCTGATACAGGTTGGTAACCAG +CCAGTAGTGCGCGTTTGATTTCCACGGATAAGACTCCGCATCCGGATACA +GGCCTCGCTTCCGGCAATACTCGTAAACCATATCAACCAGCTCGCTGACG +TTTGGCAGTCCGGCGGTAACGGATGCTTCTTCCCGGCACCATGCAACAAA +CTGCCCGGGTGATGGCAGAAATGGTCGATTCTGCCGACGGGCTACGCGCA +TTCCTGCGTTAACCTGTTCCATCGTGGTGATCCCGTTTTCCCGAAAAGCC +AGAACCCACTGGCGACGGATTTCGTTCACTTCGTTCTGGTCACGGTTTTC +CCGAAAAGCCAGAACCCACTGGCGACGGATTTCGTTCACTTCGTTCTGGT +CACGGTTAGCCAGGCTCGCCGGGAAAGTTGCCAGTAACTGGCTGAACACA +CCGTTGATGATCTGCGCTACCTGCTGTACCTGCGGCTTTTCGTCGTACTG +TTCCGGCATGTTGTTGGCGATCCGACGCATCTGCTCACGGTCAAAGTTAA +CCATCTGTGCGGCGATGTTTTTCATAGATCCACCCCGTAAATCCAGTCTG +TGTTTGTCAGGTCGAGTTTTGGTTTGCTGGCTGTCACGCCTGCCTGTTGC +TTGTTACGGTTGATTTCGAGTTGGGTCCACTTATCGCGGAGTTTGGCCGG +GCTCAGCACGTTACCGGACCAGAAGTTGTCCTGGCATGCCCAGCGGAACA +GCACACACATGTCGCGGTGGTTACGTCCGTCACGTTCACGCATCAGGCGG +ATATCGTTAGCCCACCCAGCAAAATTCGGTTTTCTGGCTGATGGTGCGAT +AGTCTTCACCATGTCAAACATCCACTCTGCGGCGGTCAGGTCTTCTGCTG +TCCCCCACTTGCTGCCGCTCTGAATTGCAGCATCCGGTTTCACCACAGAA +AGGTCGTTTTCTGGCTGGTCAGAGGATTCGCCAGAATTCTCTGACGAATA +ATCTTTTCTTTTTTCTTTTGTAATAGTGTCTTTTGTGTCCCCCTGTTTTG +AGGGATAGCAATCCCCCAATTTGAGGGATGTTTTATCCCTCGTTTTAGGG +GATTTTCCCTCGTTTTGAGGGATGCACCATTCTGAGATGTTTTTATTTGG +TCCAAACATGCCGCCTTGCTGCTTGATAATATTCATTCTGACGAGTTCTA +ACTTGGCTTCATTGCACCGTTTGACAGGTAACTTTGTAATCTCGCTAAGT +TGAGAATCGGTGATTCTGTCCATTGGTTTATTCCACCCATAGGTTTTACG +CAGACTAAGTTGAGAATCGGTGATTCTGTCCATTGGTTTATTCCACCCAT +AGGTTTTACGCAGAATGGCAAGCAGCACTTTAAACTGTCGCTTGGTCAGA +TCTGCGCCCGAATAAGCCTCAAGCAGCATATTTGATAGTCTGGCGTAACC +ATCATCGAGATCTGCCACATTACGCTCCTGTCCGGCAAAGTTACCTCTGC +CGAAGTTGAGTATTTTTGCTGTATTTGTCATAATGACTCCTGTTGATAGA +TCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTT +GCCGCCGGGCGTTTTTTATTGGTGAGAATCGCAGCAACTTGTCGCGCCAA +TCGAGCCATGTCGTCGTCAACGACCCCCCATTCAAGAACAGCAAGCAGCA +TTGAGAACTTTGGAATCCAGTCCCTCTTCCACCTGCTGATCTGCGACTTA +TCAACGCCCACAGCTTCCGCTGTCTTCTCAGTTCCAAGCATTGCGATTTT +GTTAAGCAACGCACTCTCGATTCGTAGAGCCTCGTTGCGTTTGTTTGCAC +GAACCATATGTAAGTATTTCCTTAGATAACAATTGATTGAATGTATGCAA +ATAAATGCATACACCATAGGTGTGGTTTAATTTGATGCCCTTTTTCAGGG +CTGGAATGTGTAAGAGCGGGGTTATTTATGCTGTTGTTTTTTTGTTACTC +GGGAAGGGCTTTACCTCTTCCGCATAAACGCTTCCATCAGCGTTTATAGT +TAAAAAAATCTTTCGGCCTGCATGAATGGCCTTGTTGATCGCGCTTTGAT +ATACGCCGAGATCTTTAGCTGTCTTGGTTTGCCCAAAGCGCATTGCATAA +TCTTTCAGGGTTATGCGTTGTTCCATACAACCTCCTTAGTACATGCAACC +ATTATCACCGCCAGAGGTAAAATAGTCAACACGCACGGTGTTAGATATTT +ATCCCTTGCGGTGATCACCGCCAGAGGTAAAATAGTCAACACGCACGGTG +TTAGATATTTATCCCTTGCGGTGATAGATTTAACGTATGAGCACAAAAAA +GAAACCATTAACACAAGAGCAGCTTGAGGACGCACGTCGCCTTAAAGCAA +TTTATGAAAAAAAGAAAAATGAACTTGGCTTATCCCAGGAATCTGTCGCA +GACAAGATGGGGATGGGGCAGTCAGGCGTTGGTGCTTTATTTAATGGCAT +CAATGCATTAAATGCTTATAACGCCGCATTGCTTACAAAAATTCTCAAAG +TTAGCGTTGAAGAATTTAGCCCTTCAATCGCCAGAGAAATCTACGAGATG +TATGAAGCGGTTAGTATGCAGCCGTCACTTAGAAGTGAGTATGAGTACCC +TGTTTTTTCTCATGTTCAGGCAGGGATGTTCTCACCTAAGCTAGCGAATT +CTCATGTTTGACAGCTTATCATCGATATGCTTTAATGCGGTAGTGATCAA +GAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGC +AGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCAC +AACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAG +GGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGA +ACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTC +CTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTG +CTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCC +TGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCTCCTGTCATCTC +ACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGG +CTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACA +TCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGG +ATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCC +AGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGG +CGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGAT +TCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCG +TTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCG +CTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCT +TCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGATG +TCGACAGCCCGCCTAATGAGCGGGCTTTTTTTTCCTGAGGCTGGACGACC +TCGCGGAGTTCTACCGGCAGTGCAAATCCGTCGGCATCCAGGAAACCAGC +AGCGGCTATCCGCGCATCCATGCCCCCGAACTGCAGGAGTGGGGAGGCAC +GATGGCCGCTTTGGTCCCGGATCAATTCGCGCGACCGGATCGATCCGGGC +AACGTTGTTGCCATTGCTGCAGGGGGGGGGGGGGGGGGTTCCATTGTTCA +TTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAATCCATTGT +TCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAAAAGCT +CGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTAAATAA +AAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAAT +TTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGA +TCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACA +ACGTGCGTGGAGGCCATCAAACCACGTCAAATAATCAATTATGACGCAGG +TATCGTATTAATTGATCTGCATCAACTTAACGTAAAAACAACTTCAGACA +ATACAAATCAGCGACACTGAATACGGGGCAACCTCATGTCCCCCCCCCCC +CCCCCTcCAGGCGcAGAACTGGTAGGTATGGAAGATCTCTAGAATTCAAA +AAAAGCCCGCTCATTAGGCGGGCTAATTCGAGCTCGCCCGGGGATCGATC +CTCTAGAGTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTT +CACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAG +TTAAGCCAGTATATACACTCCtgatgCGCTACGTGACTGGGTCATGGCTG +CGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTG +CTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCAT +GTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCCCAGCTGGC +TTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTTAGGATCGC +GGCCGCAGTACTGGATC \ No newline at end of file diff --git a/src/squirrel-1.4/seqs/loristB.seq b/src/squirrel-1.4/seqs/loristB.seq new file mode 100644 index 0000000..46a59d0 --- /dev/null +++ b/src/squirrel-1.4/seqs/loristB.seq @@ -0,0 +1,105 @@ +GATCCTA*GTATTCTATAGTGTCACCTAAATCGTATGTGTATGATACATAAGGTTATGTA +TTAATTGTAGCCGCGTTCTAACGACAATATGTACAAGCCTAATTGTGTAGCATCTGGCTT +ACTGAAGCAGACCCTATCATCTCTCTCGTAAACTGCCGTCAGAGTCGGTTTGGTTGGACG +AACCTTCTGAGTTTCTG*GTAACGCCGTCCCGCACCCGGAAATGGTCAGCGAACCAATCA +GCAGGGTCATCGCTAGCCAGATCCCCGCTGATTTATGCTGGTTACTGTTGCGCCTGTTAG +CGCGGCAACGTCCGGCGCACAGAAGCTATTATGCGTCCCCAGGTAATGAATAATTGCCTC +TTTGCCCGTCATACACTTGCTCCTTTCAGTCCGAACTTAGCTTTGATTTCTGCGATCTTC +GCCAGAGCCTGTGCACGATTTAGAG-TCTACCGCCCATGACAGGAAGTTGTTTTACTGGT +TCAGGGATCGCCTCACCACGGTTAATTCTCGCAGTCATATGGACAAGCTCATCTGCGGCC +TTACGG-GTAATTCCGCATCAGTAAGCGCATTGGCCCGCATGTTCTGATACAGGTTGGTA +TTACGG-GTAATTCCGCATCAGTAAGCGCATTGGCCCGCATGTTCTGATACAGGTTGGTA +ACCAGCCAGTAGTGCGCGTTTGATTTCCACGGATAAGACTCCGCATCCGGATACAGGCCT +CGCTTCCGGCAATACTCGTAAACCATATCAACCAGCTCGCTGACGTTTGGCAGTCCGGCG +GTAACGGATGCTTCTTCCCGGCACCATGCAACAAACTGCCCGGGTGATGGCAGAAATGGT +CGATTCTGCC*GACGGGCT*ACGC-CATTCCTGCGTT*AACCTGTTCCATCGTGGTGATC +CCGTTTT*CCCGAAAAGCCAGAACCCACTGGCGACGGATTTCGTT-ACTTCGTTCTGGTC +ACGGTTAGCCAGGCTCGCCGGGAAAGTTGCCAGTAACTGGCTGAACACACCGTTGATGAT +CTGCGCTACCTGCTGTA-CTGCGGCTTTTCGTCGTACTGTTCCGGCATGTTGTTGGCGAT +CCG*ACGCATCTGCTCACGGTCAAAGTTAACCATCTGTGCGGCGATGTTTTTCATAGATC +CCG*ACGCATCTGCTCACGGTCAAAGTTAACCATCTGTGCGGCGATGTTTTTCATAGATC +CACCCCGTAAATCCAGTCTGTGTTTGTCAGGTCGAGTTTTGGTTTGCTGGCTGTCACGCC +TGCCTGTTGCTTGTTACGGTTGATTTCGAGTTGGGTCCACTTATCGCGGAGTTTGGCCGG +GCTCAGCACGTTACCGGACCAGAAGTTGTCCTGGCATGCCCAGCGGAACAGCACACACAT +GTCGCGGTGGTTACGTCCGTCACGTTCACGCATCAGGCGGATATCGTTAGCCCACCCAGC +AAAATTCGGTTTTCTGGCTGATGGTGCGATAGTCTTCACCATGTCAAACATCCACTCTGC +GGCGGTCAGGTCTTCTGCTGT**CCCCCAC*TTGCTGCCGCTCTGAATTGCAGCAT-CCG +GTTTCACCACAGAAAGGTCGTTTTCTGGCTGGTCAGAGGATTCGCCAGAATTCTCTGACG +AATAATCTTTTCTTTTTTCTTTTGTAATAGTGTCTTTTGTGTCCCCCTGTTTTGAGGGAT +AGCAATCCCCCAATTTGAGGGATGTTTTATCCCTCGTTTTAGGGGATTTTCCCTCGTTTT +AGCAATCCCCCAATTTGAGGGATGTTTTATCCCTCGTTTTAGGGGATTTTCCCTCGTTTT +GAGGGATGCA*CCATTCTGAGATGTTTTTATTTGGTCCAAACATGCC*GCCTTGCTGCTT +*--ATAAT*ATTCATTCTGACGAGTTCTAACTT*GGCTTC*ATTGCACCG*TTTGACAGG +TAACTTTGTAATCTCGCT*AAGTTGAGAATCGGTGATTCTGTCCATTGGTTTATTCCACC +CATAGGTTTTACGCAGAATGGCAAGCAGCACTTT*AAACTGTCGCTTGGTCAGATCTGCG +CCCGAATAAGCCTCAAGCAGCATATTTGATAGTCTGGCGTAACCATCATCGAGATCTGCC +ACATTACGCTCCTGTCCGGCAAAGTTACCTCTGCCGAAGTTGAGTATTTTTGCTGTATTT +GTCATAATGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTT +CAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCGCAGCAACTTGTCGCG +CCAATCGAGCCA*TGTCGTCGTCAACGACCCCCCATTCAAGAACAGCAAGCAGCATTGAG +CCAATCGAGCCA*TGTCGTCGTCAACGACCCCCCATTCAAGAACAGCAAGCAGCATTGAG +AACTTTGGAATCCAGTCCCTCTTCCACCTGCTGATCTGCGACTTATCAACG-CCC-A--- +-TTCCGCTGTCTTCTCAG*TTCCAAGCATTGCGATTTTGTTAAGCAACGCACTCTCGATT +CGTAGAGCCTCGTTGCGTTTGTTTGCACGAACCATATGTAAGTATTTCCTTAGATAACAA +TTGATTGAATGTATGCAAATAAATGCATA---CCATAGGTGTGGTTTAATTTGA-TGCCC +TTTTTCAGGGCTGGAATGTGTAAGAGCGGGGTTATTTATGCTGTTGTTTTTTTGTTACTC +GGGAAGGGCTTTACCTCTTCCGCATAAACGCTTCCATCAGCGTTTATAGTTAAAAAAATC +TTTCGGCCTGCATGAATGGCCTTGTTGATCGCGCTTTGATATACGCCGAGATCTTTAGCT +GTCTTGGTTTGCCCAAAGCGCATTGCATAATCTTTCAGGGTTATGCGTTGTTCCATACAA +CCTCCTTAGTACATGCAACCATTATCACCGCCAGAGGTAAAATAGTCAACACGCACGGTG +CCTCCTTAGTACATGCAACCATTATCACCGCCAGAGGTAAAATAGTCAACACGCACGGTG +TTAGATATTTATCCCTTGCGGTGATAGATTTAACGTATGAGCACAAAAAAGAAACCATTA +ACACAAGAGCAGCTTGAGGACGCACGTCGCCTTAAAGCAATTTATGAAAAAAAGAAAAAT +GAACTTGGCTTATCCCAGGAATCTGTCGCAGCAA--ATGGGGATGGGGCAGTCAGGCGTT +GGTGCTTTATTTAATGGCATCAATGCATTAAATGCTTATAACGCCGCATT*GCTTGCAAA +AATTCTCAAAGTTAGCGTTGAAGAATTTAGCCCTTCAATCGCCAGAGAAATCTACGAGAT +GTATGAAGCGGTTAGTATGCAGCCGTCACTTAGAAGTGAGTATGAGTACCCTGTTTTTTC +TCATGTTCAGGCAGGGAT*GTTCTCACCTGAGCTTAGAACCTTTACCAAAGGT*GATGCG +GAGAGATGGGTAAGCACAACCAAAAAAGCCAGTGATTCTGCATTCTGGCTTGAGGTTGAA +GGTAATTCCATGACCGCACCAACAGGCTCCAAGCCAAGCTAGCTTCACGCT-CCGCAAGC +GGTAATTCCATGACCGCACCAACAGGCTCCAAGCCAAGCTAGCTTCACGCT-CCGCAAGC +ACTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAAC +GGTGCTGACCCCGG*ATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGC +GCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATA--TAGACTGGGCGGTT +TTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAG +CCCTGCAAAGTAAACTGGATGGCTTTCTTGCCG*CCAAGGATCTGATGGCGCAGGGGAT* +*CAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTG +CACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAG +ACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGC-C-G--TTC +TTTTTGTCAAGACCGACCTGTCCGGT-CCCTGAATGAACTGCAGGACGAGGCAGCGCGGC +TTTTTGTCAAGACCGACCTGTCCGGT-CCCTGAATGAACTGCAGGACGAGGCAGCGCGGC +TATCGTGGCTGG--------GGGCGTTCCTTGCGCA-----TGCTCGACGTTGTCACTGA +AGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCA +CCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCT +TGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTAC +TCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAG-AGCAT*CAG--G-TC +GCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCG*AGGATCTCGT +CGTGACCCAT-GCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGG +ATTCATCGACTGTGGCCGGCTGGGTGTG-CGGACCGCTATCAGGACATAGCGTTGGCTAC +CCGTGATATTG--GAAGAGCTT-GCGGC****GAATGGGCTGACCGCTTCCTCGTGCTTT +ACGGTATCGCCGCTCCCGATTCGCAGCGCAT-GCCTTCTATCGCCTTCTTGACGAGTTCT +TCTGAGCGGGACTCT-GGG-TTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCAC +TCTGAGCGGGACTCT-GGG-TTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCAC +GAGATTTCGATTCCACCGCCG*CCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGG +GA-GCCG-C-TGGATGA-CCTCCAGCGCGGGGATCTCATGCTGGAGTTCTT-GCCCACCC +CGGGCTCGA-CCCCTCGCGAGTTGGTTCAGCTGCTGCCT-AGGCTGG-ACGA-CTCGCGG +AGTTCTACCGGCAGTGCAAATCCGTCGGCATCCAGGAAACCAGCAGCGGCTATCCGCGC* +ATCCATGCCCCCGAACTGCAGGAGTGGGGAGGCACGATGGCCGCTTTGGTC--GGATCAA +TTCGCGCGA-CGGATCGATCCGGGCAACGTTGTT--CATTGCTGCAGGGGGGGGGGGGGG +GGGGGGGGGGGG--G--TTCCATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGA +CAGAGGCCAAAAAGC-TC-CTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTT +TAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTT +TAAATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGGAAAATTTT +CATAAATAGCGAAAACCCGCGAGGTCG-CGCCCCGTAACCTGTCGGAT-ACCGGAAAGGA +CCCGTAAAGTGATAATGATTATCATCTACATA-TCACAACGTGCGTGGAGGCCATCAAAC +CACGTCAAATAATCAATTATGACGCAGGTATCGTATTAATTGATCTGCATCAACTTAACG +TAAAAACAACTTCAGACAATACAAATCAGCGACACTGAATACGGGGCAACCTCATGTCAA +CCCCCCCCCCCCCCCCCCCCCCCCCCTGCAGGCGGAGAACTGGTAGGTATGGAAGATCTC +TAGAGAATTCAAAAAAAGCCCGCTCATTAGGCGGGCTAATTCGAGCTCGCCCGGGGATCG +ATCCTCTAGAGTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCG +CATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATATACA +CTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTG +ACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCT +CCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCCCAGCT +GGCTTATCGAAATTAATACGACTCACTATAGGGAGACCGGAAGCTTAGGATCGCGGCCGC +AGTACT diff --git a/src/squirrel-1.4/seqs/m13mp18.vec b/src/squirrel-1.4/seqs/m13mp18.vec new file mode 100644 index 0000000..25341c9 --- /dev/null +++ b/src/squirrel-1.4/seqs/m13mp18.vec @@ -0,0 +1,123 @@ +<---m13mp.001------> +;M13MP18 cloning vector from vecbase + AATGCTACTACTATTAGTAGAATTGATGCCACCTTTTCAGCTCGCGCCCCAAATGAAAAT + ATAGCTAAACAGGTTATTGACCATTTGCGAAATGTATCTAATGGTCAAACTAAATCTACT + CGTTCGCAGAATTGGGAATCAACTGTTACATGGAATGAAACTTCCAGACACCGTACTTTA + GTTGCATATTTAAAACATGTTGAGCTACAGCACCAGATTCAGCAATTAAGCTCTAAGCCA + TCCGCAAAAATGACCTCTTATCAAAAGGAGCAATTAAAGGTACTCTCTAATCCTGACCTG + TTGGAGTTTGCTTCCGGTCTGGTTCGCTTTGAAGCTCGAATTAAAACGCGATATTTGAAG + TCTTTCGGGCTTCCTCTTAATCTTTTTGATGCAATCCGCTTTGCTTCTGACTATAATAGT + CAGGGTAAAGACCTGATTTTTGATTTATGGTCATTCTCGTTTTCTGAACTGTTTAAAGCA + TTTGAGGGGGATTCAATGAATATTTATGACGATTCCGCAGTATTGGACGCTATCCAGTCT + AAACATTTTACTATTACCCCCTCTGGCAAAACTTCTTTTGCAAAAGCCTCTCGCTATTTT + GGTTTTTATCGTCGTCTGGTAAACGAGGGTTATGATAGTGTTGCTCTTACTATGCCTCGT + AATTCCTTTTGGCGTTATGTATCTGCATTAGTTGAATGTGGTATTCCTAAATCTCAACTG + ATGAATCTTTCTACCTGTAATAATGTTGTTCCGTTAGTTCGTTTTATTAACGTAGATTTT + TCTTCCCAACGTCCTGACTGGTATAATGAGCCAGTTCTTAAAATCGCATAAGGTAATTCA + CAATGATTAAAGTTGAAATTAAACCATCTCAAGCCCAATTTACTACTCGTTCTGGTGTTT + CTCGTCAGGGCAAGCCTTATTCACTGAATGAGCAGCTTTGTTACGTTGATTTGGGTAATG + AATATCCGGTTCTTGTCAAGATTACTCTTGATGAAGGTCAGCCAGCCTATGCGCCTGGTC + TGTACACCGTTCATCTGTCCTCTTTCAAAGTTGGTCAGTTCGGTTCCCTTATGATTGACC + GTCTGCGCCTCGTTCCGGCTAAGTAACATGGAGCAGGTCGCGGATTTCGACACAATTTAT + CAGGCGATGATACAAATCTCCGTTGTACTTTGTTTCGCGCTTGGTATAATCGCTGGGGGT + CAAAGATGAGTGTTTTAGTGTATTCTTTCGCCTCTTTCGTTTTAGGTTGGTGCCTTCGTA + GTGGCATTACGTATTTTACCCGTTTAATGGAAACTTCCTCATGAAAAAGTCTTTAGTCCT + CAAAGCCTCTGTAGCCGTTGCTACCCTCGTTCCGATGCTGTCTTTCGCTGCTGAGGGTGA + CGATCCCGCAAAAGCGGCCTTTAACTCCCTGCAAGCCTCAGCGACCGAATATATCGGTTA + TGCGTGGGCGATGGTTGTTGTCATTGTCGGCGCAACTATCGGTATCAAGCTGTTTAAGAA + ATTCACCTCGAAAGCAAGCTGATAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTT + TTTTTGGAGATTTTCAACGTGAAAAAATTATTATTCGCAATTCCTTTAGTTGTTCCTTTC + TATTCTCACTCCGCTGAAACTGTTGAAAGTTGTTTAGCAAAACCCCATACAGAAAATTCA + TTTACTAACGTCTGGAAAGACGACAAAACTTTAGATCGTTACGCTAACTATGAGGGTTGT + CTGTGGAATGCTACAGGCGTTGTAGTTTGTACTGGTGACGAAACTCAGTGTTACGGTACA + TGGGTTCCTATTGGGCTTGCTATCCCTGAAAATGAGGGTGGTGGCTCTGAGGGTGGCGGT + TCTGAGGGTGGCGGTTCTGAGGGTGGCGGTACTAAACCTCCTGAGTACGGTGATACACCT + ATTCCGGGCTATACTTATATCAACCCTCTCGACGGCACTTATCCGCCTGGTACTGAGCAA + AACCCCGCTAATCCTAATCCTTCTCTTGAGGAGTCTCAGCCTCTTAATACTTTCATGTTT + CAGAATAATAGGTTCCGAAATAGGCAGGGGGCATTAACTGTTTATACGGGCACTGTTACT + CAAGGCACTGACCCCGTTAAAACTTATTACCAGTACACTCCTGTATCATCAAAAGCCATG + TATGACGCTTACTGGAACGGTAAATTCAGAGACTGCGCTTTCCATTCTGGCTTTAATGAA + GATCCATTCGTTTGTGAATATCAAGGCCAATCGTCTGACCTGCCTCAACCTCCTGTCAAT + GCTGGCGGCGGCTCTGGTGGTGGTTCTGGTGGCGGCTCTGAGGGTGGTGGCTCTGAGGGT + GGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTTCCGGTGGTGGCTCTGGTTCCGGT + GATTTTGATTATGAAAAGATGGCAAACGCTAATAAGGGGGCTATGACCGAAAATGCCGAT + GAAAACGCGCTACAGTCTGACGCTAAAGGCAAACTTGATTCTGTCGCTACTGATTACGGT + GCTGCTATCGATGGTTTCATTGGTGACGTTTCCGGCCTTGCTAATGGTAATGGTGCTACT + GGTGATTTTGCTGGCTCTAATTCCCAAATGGCTCAAGTCGGTGACGGTGATAATTCACCT + TTAATGAATAATTTCCGTCAATATTTACCTTCCCTCCCTCAATCGGTTGAATGTCGCCCT + TTTGTCTTTAGCGCTGGTAAACCATATGAATTTTCTATTGATTGTGACAAAATAAACTTA + TTCCGTGGTGTCTTTGCGTTTCTTTTATATGTTGCCACCTTTATGTATGTATTTTCTACG + TTTGCTAACATACTGCGTAATAAGGAGTCTTAATCATGCCAGTTCTTTTGGGTATTCCGT + TATTATTGCGTTTCCTCGGTTTCCTTCTGGTAACTTTGTTCGGCTATCTGCTTACTTTTC + TTAAAAAGGGCTTCGGTAAGATAGCTATTGCTATTTCATTGTTTCTTGCTCTTATTATTG + GGCTTAACTCAATTCTTGTGGGTTATCTCTCTGATATTAGCGCTCAATTACCCTCTGACT + TTGTTCAGGGTGTTCAGTTAATTCTCCCGTCTAATGCGCTTCCCTGTTTTTATGTTATTC + TCTCTGTAAAGGCTGCTATTTTCATTTTTGACGTTAAACAAAAAATCGTTTCTTATTTGG + ATTGGGATAAATAATATGGCTGTTTATTTTGTAACTGGCAAATTAGGCTCTGGAAAGACG + CTCGTTAGCGTTGGTAAGATTCAGGATAAAATTGTAGCTGGGTGCAAAATAGCAACTAAT + CTTGATTTAAGGCTTCAAAACCTCCCGCAAGTCGGGAGGTTCGCTAAAACGCCTCGCGTT + CTTAGAATACCGGATAAGCCTTCTATATCTGATTTGCTTGCTATTGGGCGCGGTAATGAT + TCCTACGATGAAAATAAAAACGGCTTGCTTGTTCTCGATGAGTGCGGTACTTGGTTTAAT + ACCCGTTCTTGGAATGATAAGGAAAGACAGCCGATTATTGATTGGTTTCTACATGCTCGT + AAATTAGGATGGGATATTATTTTTCTTGTTCAGGACTTATCTATTGTTGATAAACAGGCG + CGTTCTGCATTAGCTGAACATGTTGTTTATTGTCGTCGTCTGGACAGAATTACTTTACCT + TTTGTCGGTACTTTATATTCTCTTATTACTGGCTCGAAAATGCCTCTGCCTAAATTACAT + GTTGGCGTTGTTAAATATGGCGATTCTCAATTAAGCCCTACTGTTGAGCGTTGGCTTTAT + ACTGGTAAGAATTTGTATAACGCATATGATACTAAACAGGCTTTTTCTAGTAATTATGAT + TCCGGTGTTTATTCTTATTTAACGCCTTATTTATCACACGGTCGGTATTTCAAACCATTA + AATTTAGGTCAGAAGATGAAATTAACTAAAATATATTTGAAAAAGTTTTCTCGCGTTCTT + TGTCTTGCGATTGGATTTGCATCAGCATTTACATATAGTTATATAACCCAACCTAAGCCG + GAGGTTAAAAAGGTAGTCTCTCAGACCTATGATTTTGATAAATTCACTATTGACTCTTCT + CAGCGTCTTAATCTAAGCTATCGCTATGTTTTCAAGGATTCTAAGGGAAAATTAATTAAT + AGCGACGATTTACAGAAGCAAGGTTATTCACTCACATATATTGATTTATGTACTGTTTCC + ATTAAAAAAGGTAATTCAAATGAAATTGTTAAATGTAATTAATTTTGTTTTCTTGATGTT + TGTTTCATCATCTTCTTTTGCTCAGGTAATTGAAATGAATAATTCGCCTCTGCGCGATTT + TGTAACTTGGTATTCAAAGCAATCAGGCGAATCCGTTATTGTTTCTCCCGATGTAAAAGG + TACTGTTACTGTATATTCATCTGACGTTAAACCTGAAAATCTACGCAATTTCTTTATTTC + TGTTTTACGTGCTAATAATTTTGATATGGTTGGTTCAATTCCTTCCATAATTCAGAAGTA + TAATCCAAACAATCAGGATTATATTGATGAATTGCCATCATCTGATAATCAGGAATATGA + TGATAATTCCGCTCCTTCTGGTGGTTTCTTTGTTCCGCAAAATGATAATGTTACTCAAAC + TTTTAAAATTAATAACGTTCGGGCAAAGGATTTAATACGAGTTGTCGAATTGTTTGTAAA + GTCTAATACTTCTAAATCCTCAAATGTATTATCTATTGACGGCTCTAATCTATTAGTTGT + TAGTGCACCTAAAGATATTTTAGATAACCTTCCTCAATTCCTTTCTACTGTTGATTTGCC + AACTGACCAGATATTGATTGAGGGTTTGATATTTGAGGTTCAGCAAGGTGATGCTTTAGA + TTTTTCATTTGCTGCTGGCTCTCAGCGTGGCACTGTTGCAGGCGGTGTTAATACTGACCG + CCTCACCTCTGTTTTATCTTCTGCTGGTGGTTCGTTCGGTATTTTTAATGGCGATGTTTT + AGGGCTATCAGTTCGCGCATTAAAGACTAATAGCCATTCAAAAATATTGTCTGTGCCACG + TATTCTTACGCTTTCAGGTCAGAAGGGTTCTATCTCTGTTGGCCAGAATGTCCCTTTTAT + TACTGGTCGTGTGACTGGTGAATCTGCCAATGTAAATAATCCATTTCAGACGATTGAGCG + TCAAAATGTAGGTATTTCCATGAGCGTTTTTCCTGTTGCAATGGCTGGCGGTAATATTGT + TCTGGATATTACCAGCAAGGCCGATAGTTTGAGTTCTTCTACTCAGGCAAGTGATGTTAT + TACTAATCAAAGAAGTATTGCTACAACGGTTAATTTGCGTGATGGACAGACTCTTTTACT + CGGTGGCCTCACTGATTATAAAAACACTTCTCAAGATTCTGGCGTACCGTTCCTGTCTAA + AATCCCTTTAATCGGCCTCCTGTTTAGCTCCCGCTCTGATTCCAACGAGGAAAGCACGTT + ATACGTGCTCGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGG + GTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTT + TCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATC + GGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTG + ATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGA + CGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACC + CTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGAACCACCATCAA + ACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGG + CCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCGCTGGTGAAAAGAAAAACCACCCT + GGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGC + ACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGC + TCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAA + TTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTCGAGC + TCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCACTGGCCGTC + GTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCA + CATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAA + CAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTG + CCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACGGTCGTCGTCCCCTCAAAC + TGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTAACCTATCCCATTACGGTC + AATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTT + GATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTCCTATTGGT + TAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTA + CAATTTAAATATTTGCTTATACAATCTTCCTGTTTTTGGGGCTTTTCTGATTATCAACCG + GGGTACATATGATTGACATGCTAGTTTTACGATTACCGTTCATCGATTCTCTTGTTTGCT + CCAGACTCTCAGGCAATGACCTGATAGCCTTTGTAGATCTCTCAAAAATAGCTACCCTCT + CCGGCATTAATTTATCAGCTAGAACGGTTGAATATCATATTGATGGTGATTTGACTGTCT + CCGGCCTTTCTCACCCTTTTGAATCTTTACCTACACATTACTCAGGCATTGCATTTAAAA + TATATGAGGGTTCTAAAAATTTTTATCCTTGCGTTGAAATAAAGGCTTCTCCCGCAAAAG + TATTACAGGGTCATAATGTTTTTGGTACAACCGATTTAGCTTTATGCTCTGAGGCTTTAT + TGCTTAATTTTGCTAATTCTTTGCCTTGCCTGTATGATTTATTGGATGTT diff --git a/src/squirrel-1.4/seqs/m13mp7.seq b/src/squirrel-1.4/seqs/m13mp7.seq new file mode 100644 index 0000000..75b10d8 --- /dev/null +++ b/src/squirrel-1.4/seqs/m13mp7.seq @@ -0,0 +1,126 @@ +<---M13MP7.001-----> +AACGCTACTACTATTAGTAGAATTGATGCCACCTTTTCAGCTCGCGCCCCAAATGAAAAT +ATAGCTAAACAGGTTATTGACCATTTGCGAAATGTATCTAATGGTCAAACTAAATCTACT +; by starting a record with ; comments can be inserted anywhere +; but the ; must appear in column 1. +; comment lines will be displayed when the file is read +CGTTCGCAGAATTGGGAATCAACTGTTACATGGAATGAAACTTCCAGACACCGTACTTTA +GTTGCATATTTAAAACATGTTGAGCTACAGCACCAGATTCAGCAATTAAGCTCTAAGCCA +; here is another comment +TCCGCAAAAATGACCTCTTATCAAAAGGAGCAATTAAAGGTACTCTCTAATCCTGACCTG +TTGGAGTTTGCTTCCGGTCTGGTTCGCTTTGAAGCTCGAATTAAAACGCGATATTTGAAG +TCTTTCGGGCTTCCTCTTAATCTTTTTGATGCAATCCGCTTTGCTTCTGACTATAATAGT +CAGGGTAAAGACCTGATTTTTGATTTATGGTCATTCTCGTTTTCTGAACTGTTTAAAGCA +TTTGAGGGGGATTCAATGAATATTTATGACGATTCCGCAGTATTGGACGCTATCCAGTCT +AAACATTTTACTATTACCCCCTCTGGCAAAACTTCTTTTGCAAAAGCCTCTCGCTATTTT +GGTTTTTATCGTCGTCTGGTAAACGAGGGTTATGATAGTGTTGCTCTTACTATGCCTCGT +AATTCCTTTTGGCGTTATGTATCTGCATTAGTTGAATGTGGTATTCCTAAATCTCAACTG +ATGAATCTTTCTACCTGTAATAATGTTGTTCCGTTAGTTCGTTTTATTAACGTAGATTTT +TCTTCCCAACGTCCTGACTGGTATAATGAGCCAGTTCTTAAAATCGCATAAGGTAATTCA +CAATGATTAAAGTTGAAATTAAACCATCTCAAGCCCAATTTACTACTCGTTCTGGTGGTT +CTCGTCAGGGCAAGCCTTATTCACTGAATGAGCAGCTTTGTTACGTTGATTTGGGTAATG +AATATCCGGTTCTTGTCAAGATTACTCTTGATGAAGGTCAGCCAGCCTATGCGCCTGGTC +TGTACACCGTTCATCTGTCCTCTTTCAAAGTTGGTCAGTTCGGTTCCCTTATGATTGACC +GTCTGCGCCTCGTTCCGGCTAAGTAACATGGAGCAGGTCGCGGATTTCGACACAATTTAT +CAGGCGATGATACAAATCTCCGTTGTACTTTGTTTCGCGCTTGGTATAATCGCTGGGGGT +CAAAGATGAGTGTTTTAGTGTATTCTTTCGCCTCTTTCGTTTTAGGTTGGTGCCTTCGTA +GTGGCATTACGTATTTTACCCGTTTAATGGAAACTTCCTCATGAAAAAGTCTTTAGTCCT +CAAAGCCTCTGTAGCCGTTGCTACCCTCGTTCCGATGCTGTCTTTCGCTGCTGAGGGTGA +CGATCCCGCAAAAGCGGCCTTTAACTCCCTGCAAGCCTCAGCGACCGAATATATCGGTTA +TGCGTGGGCGATGGTTGTTGTCATTGTCGGCGCAACTATCGGTATCAAGCTGTTTAAGAA +ATTCACCTCGAAAGCAAGCTGATAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTT +TTTTTGGAGATTTTCAACGTGAAAAAATTATTATTCGCAATTCCTTTAGTTGTTCCTTTC +TATTCTCACTCCGCTGAAACTGTTGAAAGTTGTTTAGCAAAACCCCATACAGAAAATTCA +TTTACTAACGTCTGGAAAGACGACAAAACTTTAGATCGTTACGCTAACTATGAGGGTTGT +CTGTGGAATGCTACAGGCGTTGTAGTTTGTACTGGTGACGAAACTCAGTGTTACGGTACA +TGGGTTCCTATTGGGCTTGCTATCCCTGAAAATGAGGGTGGTGGCTCTGAGGGTGGCGGT +TCTGAGGGTGGCGGTTCTGAGGGTGGCGGTACTAAACCTCCTGAGTACGGTGATACACCT +ATTCCGGGCTATACTTATATCAACCCTCTCGACGGCACTTATCCGCCTGGTACTGAGCAA +AACCCCGCTAATCCTAATCCTTCTCTTGAGGAGTCTCAGCCTCTTAATACTTTCATGTTT +CAGAATAATAGGTTCCGAAATAGGCAGGGGGCATTAACTGTTTATACGGGCACTGTTACT +CAAGGCACTGACCCCGTTAAAACTTATTACCAGTACACTCCTGTATCATCAAAAGCCATG +TATGACGCTTACTGGAACGGTAAATTCAGAGACTGCGCTTTCCATTCTGGCTTTAATGAA +GATCCATTCGTTTGTGAATATCAAGGCCAATCGTCTGACCTGCCTCAACCTCCTGTCAAT +GCTGGCGGCGGCTCTGGTGGTGGTTCTGGTGGCGGCTCTGAGGGTGGTGGCTCTGAGGGT +GGCGGTTCTGAGGGTGGCGGCTCTGAGGGAGGCGGTTCCGGTGGTGGCTCTGGTTCCGGT +GATTTTGATTATGAAAAGATGGCAAACGCTAATAAGGGGGCTATGACCGAAAATGCCGAT +GAAAACGCGCTACAGTCTGACGCTAAAGGCAAACTTGATTCTGTCGCTACTGATTACGGT +GCTGCTATCGATGGTTTCATTGGTGACGTTTCCGGCCTTGCTAATGGTAATGGTGCTACT +GGTGATTTTGCTGGCTCTAATTCCCAAATGGCTCAAGTCGGTGACGGTGATAATTCACCT +TTAATGAATAATTTCCGTCAATATTTACCTTCCCTCCCTCAATCGGTTGAATGTCGCCCT +TTTGTCTTTAGCGCTGGTAAACCATATGAATTTTCTATTGATTGTGACAAAATAAACTTA +TTCCGTGGTGTCTTTGCGTTTCTTTTATATGTTGCCACCTTTATGTATGTATTTTCTACG +TTTGCTAACATACTGCGTAATAAGGAGTCTTAATCATGCCAGTTCTTTTGGGTATTCCGT +TATTATTGCGTTTCCTCGGTTTCCTTCTGGTAACTTTGTTCGGCTATCTGCTTACTTTTC +TTAAAAAGGGCTTCGGTAAGATAGCTATTGCTATTTCATTGTTTCTTGCTCTTATTATTG +GGCTTAACTCAATTCTTGTGGGTTATCTCTCTGATATTAGCGCTCAATTACCCTCTGACT +TTGTTCAGGGTGTTCAGTTAATTCTCCCGTCTAATGCGCTTCCCTGTTTTTATGTTATTC +TCTCTGTAAAGGCTGCTATTTTCATTTTTGACGTTAAACAAAAAATCGTTTCTTATTTGG +ATTGGGATAAATAATATGGCTGTTTATTTTGTAACTGGCAAATTAGGCTCTGGAAAGACG +CTCGTTAGCGTTGGTAAGATTCAGGATAAAATTGTAGCTGGGTGCAAAATAGCAACTAAT +CTTGATTTAAGGCTTCAAAACCTCCCGCAAGTCGGGAGGTTCGCTAAAACGCCTCGCGTT +CTTAGAATACCGGATAAGCCTTCTATATCTGATTTGCTTGCTATTGGGCGCGGTAATGAT +TCCTACGATGAAAATAAAAACGGCTTGCTTGTTCTCGATGAGTGCGGTACTTGGTTTAAT +ACCCGTTCTTGGAATGATAAGGAAAGACAGCCGATTATTGATTGGTTTCTACATGCTCGT +AAATTAGGATGGGATATTATTTTTCTTGTTCAGGACTTATCTATTGTTGATAAACAGGCG +CGTTCTGCATTAGCTGAACATGTTGTTTATTGTCGTCGTCTGGACAGAATTACTTTACCT +TTTGTCGGTACTTTATATTCTCTTATTACTGGCTCGAAAATGCCTCTGCCTAAATTACAT +GTTGGCGTTGTTAAATATGGCGATTCTCAATTAAGCCCTACTGTTGAGCGTTGGCTTTAT +ACTGGTAAGAATTTGTATAACGCATATGATACTAAACAGGCTTTTTCTAGTAATTATGAT +TCCGGTGTTTATTCTTATTTAACGCCTTATTTATCACACGGTCGGTATTTCAAACCATTA +AATTTAGGTCAGAAGATGAAATTAACTAAAATATATTTGAAAAAGTTTTCTCGCGTTCTT +TGTCTTGCGATTGGATTTGCATCAGCATTTACATATAGTTATATAACCCAACCTAAGCCG +GAGGTTAAAAAGGTAGTCTCTCAGACCTATGATTTTGATAAATTCACTATTGACTCTTCT +CAGCGTCTTAATCTAAGCTATCGCTATGTTTTCAAGGATTCTAAGGGAAAATTAATTAAT +AGCGACGATTTACAGAAGCAAGGTTATTCACTCACATATATTGATTTATGTACTGTTTCC +ATTAAAAAAGGTAATTCAAATGAAATTGTTAAATGTAATTAATTTTGTTTTCTTGATGTT +TGTTTCATCATCTTCTTTTGCTCAGGTAATTGAAATGAATAATTCGCCTCTGCGCGATTT +TGTAACTTGGTATTCAAAGCAATCAGGCGAATCCGTTATTGTTTCTCCCGATGTAAAAGG +TACTGTTACTGTATATTCATCTGACGTTAAACCTGAAAATCTACGCAATTTCTTTATTTC +TGTTTTACGTGCTAATAATTTTGATATGGTTGGTTCAATTCCTTCCATAATTCAGAAGTA +TAATCCAAACAATCAGGATTATATTGATGAATTGCCATCATCTGATAATCAGGAATATGA +TGATAATTCCGCTCCTTCTGGTGGTTTCTTTGTTCCGCAAAATGATAATGTTACTCAAAC +TTTTAAAATTAATAACGTTCGGGCAAAGGATTTAATACGAGTTGTCGAATTGTTTGTAAA +GTCTAATACTTCTAAATCCTCAAATGTATTATCTATTGACGGCTCTAATCTATTAGTTGT +TAGTGCACCTAAAGATATTTTAGATAACCTTCCTCAATTCCTTTCTACTGTTGATTTGCC +AACTGACCAGATATTGATTGAGGGTTTGATATTTGAGGTTCAGCAAGGTGATGCTTTAGA +TTTTTCATTTGCTGCTGGCTCTCAGCGTGGCACTGTTGCAGGCGGTGTTAATACTGACCG +CCTCACCTCTGTTTTATCTTCTGCTGGTGGTTCGTTCGGTATTTTTAATGGCGATGTTTT +AGGGCTATCAGTTCGCGCATTAAAGACTAATAGCCATTCAAAAATATTGTCTGTGCCACG +TATTCTTACGCTTTCAGGTCAGAAGGGTTCTATCTCTGTTGGCCAGAATGTCCCTTTTAT +TACTGGTCGTGTGACTGGTGAATCTGCCAATGTAAATAATCCATTTCAGACGATTGAGCG +TCAAAATGTAGGTATTTCCATGAGCGTTTTTCCTGTTGCAATGGCTGGCGGTAATATTGT +TCTGGATATTACCAGCAAGGCCGATAGTTTGAGTTCTTCTACTCAGGCAAGTGATGTTAT +TACTAATCAAAGAAGTATTGCTACAACGGTTAATTTGCGTGATGGACAGACTCTTTTACT +CGGTGGCCTCACTGATTATAAAAACACTTCTCAAGATTCTGGCGTACCGTTCCTGTCTAA +AATCCCTTTAATCGGCCTCCTGTTTAGCTCCCGCTCTGATTCCAACGAGGAAAGCACGTT +ATACGTGCTCGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGG +GTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTT +TCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATC +GGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTG +ATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGA +CGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTTGAACAACACTCAACC +CTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGAACCACCATCAA +ACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGG +CCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCGCTGGTGAAAAGAAAAACCACCCT +GGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATCCAGCTGGC +ACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTACC +TCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAA +TTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTCCCCG +GATCCGTCGACCTGCAGGTCGACGGATCCGGGGAATTCACTGGCCGTCGTTTTACAACGT +CGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCCTTC +GCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGTAGC +CTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGG +CTGGAGTGCGATCTTCCTGAGGCCGA-AC-GTCGTCGTCCCCTCAAACTGGCAGATGCAC +GGTTACGATGCGCCCATCTACACCAACGTAACCTATCCCATTACGGTCAATCCGCCGTTT +GTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGG +CTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTCCTATTGGTTAAAAAATGAGC +TGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTAAATAT +TTGCTTATACAATCTTCCTGTTTTTGGGGCTTTTCTGATTATCAACCGGGGTACATATGA +TTGACATGCTAGTTTTACGATTACCGTTCATCGATTCTCTTGTTTGCTCCAGACTCTCAG +GCAATGACCTGATAGCCTTTGTAGATCTCTCAAAAATAGCTACCCTCTCCGGCATGAATT +TATCAGCTAGAACGGTTGAATATCATATTGATGGTGATTTGACTGTCTCCGGCCTTTCTC +ACCCTTTTGAATCTTTACCTACACATTACTCAGGCATTGCATTTAAAATATATGAGGGTT +CTAAAAATTTTTATCCTTGCGTTGAAATAAAGGCTTCTCCCGCAAAAGTATTACAGGGTC +ATAATGTTTTTGGTACAACCGATTTAGCTTTATGCTCTGAGGCTTTATTGCTTAATTTTG +CTAATTCTTTGCCTTGCCTGTATGATTTATTGGATGTT diff --git a/src/squirrel-1.4/seqs/pYAC4.seq b/src/squirrel-1.4/seqs/pYAC4.seq new file mode 100644 index 0000000..5cc7e6e --- /dev/null +++ b/src/squirrel-1.4/seqs/pYAC4.seq @@ -0,0 +1,225 @@ +CTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGC +TCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAA +GCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCAT +GACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGC +ATCAGAGCAGATTGTACTGAGAGTGCACCATATGCGGTGTGAAATACCGC +ACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCG +CTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGC +TCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG +GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAG +GCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCA +CAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA +GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCG +ACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGT +GGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG +TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGC +TGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGA +CTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT +ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTAC +ACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTT +CGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA +GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGA +TCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAA +CGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT +TCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGT +ATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC +ACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCC +CCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGT +GCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGC +AATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTT +TATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGT +AGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCAT +CGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCC +AACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTT +AGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTT +ATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCAT +CCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGA +GAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGA +TAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAAC +GTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGT +TCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTT +CACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAA +AGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTT +CAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACAT +ATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTC +CCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTA +ACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAATTAAT +TCGGTCGAAAAAAGAAAAGGAGAGGGCCAAGAGGGAGGGCATTGGTGACT +ATTGAGCACGTGAGTATACGTGATTAAGCACACAAAGGCAGCTTGGAGTA +TGTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGC +GGCTTGCAGAGCACAGAGGCCGCAGAATGTGCTCTAGATTCCGATGCTGA +CTTGCTGGGTATTATATGTGTGCCCAATAGAAAGAGAACAATTGACCCGG +TTATTGCAAGGAAAATTTCAAGTCTTGTAAAAGCATATAAAAATAGTTCA +GGCACTCCGAAATACTTGGTTGGCGTGTTTCGTAATCAACCTAAGGAGGA +TGTTTTGGCTCTGGTCAATGATTACGGCATTGATATCGTCCAACTGCATG +GAGATGAGTCGTGGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATT +AAAAGACTCGTATTTCCAAAAGACTGCAACATACTACTCAGTGCAGCTTC +ACAGAAACCTCATTCGTTTATTCCCTTGTTTGATTCAGAAGCAGGTGGGA +CAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTGGAAGGCAA +GAGAGCCCCGAAAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGA +AAATGTTGGTGATGCGCTTAGATTAAATGGCGTTATTGGTGTTGATGTAA +GCGGAGGTGTGGAGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAAT +TTCGTCAAAAATGCTAAGAAATAGGTTATTACTGAGTAGTATTTATTTAA +GTATTGTTTGTGCACTTGCCTGCAGGCCTTTTGAAAAGCAAGCATAAAAG +ATCTAAACATAAAATCTGTAAAATAACAAGATGTAAAGATAATGCTAAAT +CATTTGGCTTTTTGATTGATTGTACAGGAAAATATACATCGCAGGGGGTT +GACTTTTACCATTTCACCGCAATGGAATCAAACTTGTTGAAGAGAATGTT +CACAGGCGCATACGCTACAATGACCCGATTCTTGCTAGCCTTTTCTCGGT +CTTGCAAACAACCGCCGGCAGCTTAGTATATAAATACACATGTACATACC +TCTCTCCGTATCCTCGTAATCATTTTCTTGTATTTATCGTCTTTTCGCTG +TAAAAACTTTATCACACTTATCTCAAATACACTTATTAACCGCTTTTACT +ATTATCTTCTACGCTGACAGTAATATCAAACAGTGACACATATTAAACAC +AGTGGTTTCTTTGCATAAACACCATCAGCCTCAAGTCGTCAAGTAAAGAT +TTCGTGTTCATGCAGATAGATAACAATCTATATGTTGATAATTAGCGTTG +CCTCATCAATGCGAGATCCGTTTAACCGGACCCTAGTGCACTTACCCCAC +GTTCGGTCCACTGTGTGCCGAACATGCTCCTTCACTATTTTAACATGTGC +TAAATCCTCTTTATATGATCTGCCGATAGATAGTTCTAAGTCATTGAGGT +TCATCAACAATTGGATTTTCTGTTTACTCGACTTCAGGTAAATGAAATGA +GATGATACTTGCTTATCTCATAGTTAACTCTAAGAGGTGATACTTATTTA +CTGTAAAACTGTGACGATAAAACCGGAAGGAAGAATAAGAAAACTCGAAC +TGATCTATAATGCCTATTTTCTGTAAAGAGTTTAAGCTATGAAAGCCTCG +GCATTTTGGCCGATCCTAGGTAGTGCTTTTTTTCCAAGGACAAAACAGTT +TCTTTTTCTTGAGCAGGTTTTATGTTTCGGTAATCATAAACAATAAATAA +ATTATTTCATTTATGTTTAAAAATAAAAAATAAAAAAGTATTTTAAATTT +TTAAAAAAGTTGATTATAAGCATGTGACCTTTTGCAAGCAATTAAATTTT +GCAATTTGTGATTTTAGGCAAAAGTTACAATTTCTGGCTCGTGTAATATA +TGTATGCTAAAGTGAACTTTTACAAAGTCGATATGGACTTAGTCAAAAGA +AATTTTCTTAAAAATATATAGCACTAGCCAATTTAGCACTTCTTTATGAG +ATATATTATAGACTTTATTAAGCCAGATTTGTGTATTATATGTATTTACC +CGGCGAATCATGGACATACATTCTGAAATAGGTAATATTCTCTATGGTGA +GACAGCATAGATAACCTAGGATACAAGTTAAAAGCTAGTACTGTTTTGCA +GTAATTTTTTTCTTTTTTATAAGAATGTTACCACCTAAATAAGTTATAAA +GTCAATAGTTAAGTTTGATATTTGATTGTAAAATACCGTAATATATTTGC +ATGATCAAAAGGCTCAATGTTGACTAGCCAGCATGTCAACCACTATATTG +ATCACCGATATATGGACTTCCACACCAACTAGTAATATGACAATAAATTC +AAGATATTCTTCATGAGAATGGCCCAGCTGTTCCTTCAACATTTGAGAGA +TCTGAAATGACCTCGAGTCTTTGATTTAAAAGTGAAACGCGCTGATTGAT +CTCTAAGTAACCTCTTGTTGCTTGGTATATGGGTTCTAACTGCGGTTCTG +ACCACATAATTTCTGGAGAGTCTAAAACTGATCCATGTAGATTGATATTT +ATCCTCAAAATGAATAGCTCCCCTATACTTTTCATTATATCTTCTTTGCT +CATAGAAACTTTACCGCTGTATGCAATTTCTTGTGGTATATCCTGAGTAT +CTTCAATAGTATTATCCACAAGCTCTTCGAAAAGTGAAATTTTTACGCTT +TGAGCAATTGCATGAGCTCATGTTTGACAGCTTATCATCGATAAGCTTTA +ATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTA +TGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGAT +GCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGA +TATCGTCCATTCCGACAGCATCGCCAGTCACTATGGCGTGCTGCTAGCGC +TATATGCGTTGATGCAATTTCTATGCGCACCCGTTCTCGGAGCACTGTCC +GACCGCTTTGGCCGCCGCCCAGTCCTGCTCGCTTCGCTACTTGGAGCCAC +TATCGACTACGCGATCATGGCGACCACACCCGTCCTGTGGATCAATTCTT +TAGTATAAATTTCACTCTGAACCATCTTGGAAGGACCGGATAATTATTTG +AAATCTCTTTTTCAATTGTATATGTGTTATGTAGTATACTTTGTCTTCAA +CAATTAAATACTCTCGGTAGCCAAGTTGGTTTAAGGCGCAAGACTGTATT +TTATCACTACGGAATTCCGTAATCTTGAGATCGGGCGTTCGACTCGCCCC +CGGGAGATTTTTTTGTTTTTTTATGTCTCCATTCACTTCCCAGACTTGCA +AGTTGAAATATTTCTTTCAAGCTCTACGCCGGACGCATCGTGGCCGGCAT +CACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCG +ATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGC +GTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTC +CTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTAC +TACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGACCG +ATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGG +CATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCG +TAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTT +CGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTT +GCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCG +GCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTAC +GTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGAT +TCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGT +CCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCG +GCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGAT +TTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCG +CCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGC +CGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTC +ACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGC +GCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCA +GCCGCACGCGGCGCATCCCCCCCCCCCTTTCAATTCAATTCATCATTTTT +TTTTTATTCTTTTTTTTGATTTCGGTTTCTTTGAAATTTTTTTGATTCGG +TAATCTCCGAACAGAAGGAAGAACGAAGGAAGGAGCACAGACTTAGATTG +GTATATATACGCATATGTAGTGTTGAAGAAACATGAAATTGCCCAGTATT +CTTAACCCAACTGCACAGAACAAAAACCTGCAGGAAACGAAGATAAATCA +TGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTT +GCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGC +TTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCAT +TAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGAT +TTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTA +CAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAG +TCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGAC +ATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAA +GCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAG +CAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGT +ACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGC +TCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGA +CACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTAT +AGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGG +AAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTT +ACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAAC +TAAAAAACTGTATTATAAGTAAATGCATGTATACTAAACTCACAAATTAG +AGCTTCAATTTAATTATATCAGTTATTACGAATCTCGGTCGTAATGATTT +TTATAATGACGAAAAAAAAAAAATTGGAAAGAAAAGGGGGGGGGGGCAGC +GTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGAC +CCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGA +TACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGA +GCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAAC +GCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGAT +GCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGG +CATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAG +TTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAA +CCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATG +AACAGAAATTCCCCCTTACACGGAGGCATCAAGTGACCAAACAGGAAAAA +ACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCT +GGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAAT +CGCTTCACGACCACGCTGATGAGCTTTACCGCAGCCCTCGAGGGCAAGCT +TGCATTTTTAGATAAAATTTATTAATCATCATTAATTTCTTGAAAAACAT +TTTATTTATTGATCTTTTATAACAAAAAACCCTTCTAAAAGTTTATTTTT +GAATGAAAAACTTATAAAAATTTATGAAAACTACAAAAAATAAAATTTTT +AATTAAAATAATTTTGATAAGAACTTCAATCTTTGACTAGCTAGCTTAGT +CATTTTTGAGATTTAATTAATATTTTATGTTTATTCATATATAAACTATT +CAAAATATTATAGAATTTAAACATTTTAACATCTTAATCATTCATAAATA +ACTAAAAATCAAAGTATTACATCAATAAATAACTTTTACTCAATGTCAAA +GAAATTATTTGGGGTTGGGGTTGGGGTTGGGGTTGGGGTTGGGGGATCCG +CTGCACGGDCCTGTTCCCTAGCATGTACGTGAGCGTATTTCCTTTTAAAC +CACGACGCTTTGTCTTCATTCAACGTTTCCCATTGTTTTTTTCTACTATT +GCTTTGCTGTGGGAAAAACTTATCGAAAGATGACGACTTTTTCTTAATTC +TCGTTTTAAGAGCTTGGTGAGCGCTAGGAGTCACTGCCAGGTATCGTTTG +AACACGGCATTAGTCAGGGAAGTCATAACACAGTCCTTTCCCGCAATTTT +CTTTTTCTATTACTCTTGGCCTCCTCTAGTACACTCTATATTTTTTTATG +CCTCGGTAATGATTTTCATTTTTTTTTTTCCACCTAGCGGATGACTCTTT +TTTTTTCTTAGCGATTGGCATTATCACATAATGAATTATACATTATATAA +AGTAATGTGATTTCTTCGAAGAATATACTAAAAAATGAGCAGGCAAGATA +AACGAAGGCAAAGATGACAGAGCAGAAAGCCCTAGTAAAGCGTATTACAA +ATGAAACCAAGATTCAGATTGCGATCTCTTTAAAGGGTGGTCCCCTAGCG +ATAGAGCACTCGATCTTCCCAGAAAAAGAGGCAGAAGCAGTAGCAGAACA +GGCCACACAATCGCAAGTGATTAACGTCCACACAGTGATAGGGTTTCTGG +ACCATATGATACATGCTCTGGCCAAGCATTCCGGCTGGTCGCTAATCGTT +GAGTGCATTGGTGACTTACACATAGACGACCATCACACCACTGAAGACTG +CGGGATTGCTCTCGGTCAAGCTTTTAAAGAGGCCCTACTGGCGCGTGGAG +TAAAAAGGTTTGGATCAGGATTTGCGCCTTTGGATGAGGCACTTTCCAGA +GCGGTGGTAGATCTTTCGAACAGGCCGTACGCAGTTGTCGAACTTGTTTT +GCAAAGGGAGAAAGTAGGAGATCTCTCTTGCGAGATGATCCCGCATTGTC +TTGAAAGCTTTGCAGAGGCTAGCAGAATTACCCTCCACGTTGATTGTCTG +CGAGGCAAGAATGATCATCACCGTAGTGAGAGTGCGTTCAAGGCTCTTGC +GGTTGCCATAAGAGAAGCCACCTCGCCCAATGGTACCAACGATGTTCCCT +CCACCAAAGGTGTTCTTATGTAGTGACACCGATTATTTAAAGCTGCAGCA +TACGATATATATACATGTGTATATATGTATACCTATGAATGTCAGTAAGT +ATGTATACGAACAGTATGATACTGAAGATGACAAGGTAATGCATCATTCT +ATACGTGTCATTCTGAACGAGGCGCGCTTTCCTTTTTTCTTTTTGCTTTT +TCTTTTTTTTTCTCTTGAACTCGAGAAAAAAAATATAAAAGAGATGGAGG +AACGGGAAAAAGTTAGTTGTGGTGATAGGTGGCAAGTGGTATTCCGTAAG +AACAACAAGAAAAGCATTTCATATTATGGCTGAACTGAGCGAACAAGTGC +AAAATTTAAGCATCAACGACAACAACGAGAATGTGTATGTTCCTCCTCAC +TTAAGAGGAAAACCAAGAAGTGCCAGAAATAACATGAGCAACTACAATAA +CAACAACGGCGGCTACAACGGTGGCCGTGGCGGTGGCAGCTTCTTTAGCA +ACAACCGTCGTGGTGGTTACGGCAACGGTGGTTTCTTCGGTGGAAACAAC +GGTGGCAGCAGATCTAACGGCCGTTCTGGTGGTAGATGGATATGGCAAAC +ATGTCCCAGTCCCAAGAAACGAAAAGGCCGAGATCGCCATATTTGGTGTC +CCCGAGGATCCCCCCAACCCCAACCCCAACCCCAACCCCAACCCCAAATA +ATTTCTTTGACATTGAGTAAAAGTTATTTATTGATGTAATACTTTGATTT +TTAGTTATTTATGAATGATTAAGATGTTAAAATGTTTAAATTCTATAATA +TTTTGAATAGTTTATATATGAATAAACATAAAATATTAATTAAATCTCAA +AAATGACTAAGCTAGCTAGTCAAAGATTGAAGTTCTTATCAAAATTATTT +TAATTAAAAATTTTATTTTTTGTAGTTTTCATAAATTTTTATAAGTTTTT +CATTCAAAAATAAACTTTTAGAAGGGTTTTTTGTTATAAAAGATCAATAA +ATAAAATGTTTTTCAAGAAATTAATGATGATTAATAAATTTTATCTAAAA +ATGCAAGCTTGCCCTCGAGGG + diff --git a/src/squirrel-1.4/seqs/pjb8.vep b/src/squirrel-1.4/seqs/pjb8.vep new file mode 100644 index 0000000..ddcad83 --- /dev/null +++ b/src/squirrel-1.4/seqs/pjb8.vep @@ -0,0 +1,93 @@ +; pjb8 sequence from karens r107 database 4-10-91 + GAATTCTCATGTTTGACAGCTTATCATCGATAAGCTTTAA + TGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAAC + AATGCGCTCATCGTCATCCTCGGCACCGTCACCCT-GGATGCTGTAGGCATAGGCTTGGT + TATGCCGGTACTGCCGGGCCTCTTGCGGGATATCGTCCATTCCGACAGCATCGCCAGTCA + CTATGGCGTGCTGCTAGCGCTATATGCGTTGATGCAATTTCTATGCGCACCCGTTCTCGG + AGCACTGTCCGACCGCTTTGGCCGCCGCC-AGTCCTGCTCGC-TTCGCTACTTGGAGCCA + CTATCGACTACGCGATCATGGCGACCACACCCGTCCTGTGGATCTGCCTCGCTGGCCTGC + CGCAGTTCTTCAACCTCCCGGCGCA-GTTTTTCGTTTCTCAATTTCAGCATCC-TTTCGG + CATACCATTTTATGACGGCGGCAGAGTCATAAAGCACCTCATTACCCTTGCCACCGCCTC + GCAGAACGGGCATTCCCTGTTCCTGCCAGTTCTGAATGGTAC*GGAT-ACTCGCACCGAA + AATGTCAGCC-AGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAG + AAAGGA-ACGACAGAGGCCAAAAAGCTC--TTTCAGCACC-TGTCGTTTCCTTTTC--TT + TTCAGAGGGTATTTTAAATAAAAACATTAAGT*TATGACGAAGAAGAACGGAAACGCCTT + AA-ACCGGAAAATTTTCATAAATAGCGAAA-CCCGCGAGTCGCCGCCCCGTAACAAGGCG + GATCC-*GAGAAGGACCCGCAAATGATAATAATTATCAATTGCATACTATCGACGGCACT + GCTGCCAGATAACACCACCGGGGAAACATTCCATCATGATGGCCGTGCGGACATAGGAAG + CCAGTTCATCCATCGCTTTCTTGTCT-CTGCCATTTGCTTTGTGACATCCAGCGCCGCAC + ATTCAGCAGCGTTTTTCAGCGCGTTTTCGATCAACGTTTCAA*TGTTGGTATCAACA-CC + AGG-TTTAACTTT--*-----TTATCGGCACTGACGGTTACCTTGTTCTGCG**CTGGCT + CATCACGCTGGATACCAAGGCTGATG-TT*GTA*GATATTGGTCAC*CGGCTGAGGTGT* + TTCGATTGCCGCTGCGTGGATAGCACCATTTGCGATAG----CGTCCTTGATGAATGACA + CTCCATTGCGAATAAGTTCGAAGGAGACGGTGTCACGAATGCGCTGGTCCAGCTCGTCGA + TTGCCTTTTGTGCAGCAGAGGTATCAATCT-CAAC-CCAAGC-TCATCGAAGCGCAATAT + TGCTGCTCACCAAAACGCGTATTGACCAGGTGTTCAACGGCAAATTTCTGCCCTTCTGAT + GTCAGAAAGGTAAAGTGATTTTCTTTCTGGTATTCAGTTGCTGT-GTGTCTGGTTTCAGC + AAA*--CCAAG-TCGCGCAATTCGGCTGTGCAGTATTTAGAAGGCAGATCACCAGACAGC + AACGGC-CACGGAAAAACAGCGCATACAGAACATCCGTCGCCGCGCCGGACAACGTGATA + ATTTTATGACCCATGATTTATTTCCTTTTAGACGTGAGCCTGTCGCACAGCAAAGCCGCC + GAAAGTTAACGGTTTGCCCAGGCTCA-CAACTGAAAGACTTTCT-ACGGT-GTGCGCGTG + CGATGCG--TAGAAGACTGATTTATCAACCTGTCTTTATATCAGGATTCATTACCTGACT + ATTTGTGGGTAAAGTTCGTAGTGCGCTGATCGTGCAAAATGATTTTAGTTGGGAACAGTT + CGCAACTCTGTCCCATAAAAATCAGCATATTCCCATCTATCCCATATCCAGCGCATTGAC + CATCG*GGATACTGAAGGGAGATTCCATCATCTCTTAGAAAGATCACCATCTCTTTTGTT + TCAATTTGCATATAGCTACCTGGAGGATTTATGAATACAAGGATTTTCATG-ACTATTAC + CATGAGATT-GATTTTCCATCTTTATTCGCGAGA-CAGT-GGAAAGCGATGACGATG-TG + GGTACTACATTGCGCATTCACCTACT-TGTGAGCGCATG*GTCGAAGCATGG-ATATG-- + CATGCTGTGACTGCCAAGATCCTCTAC-CCGGACGCATCGTGGCCA-TTCACCGGGCCAC + AGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCA + CTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCG-GGG- + ACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGGTGC-TCAACGGC + CTCAACCTACTACTGG-TGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGACCGA + TGCCCTTGAGAGCCTTCAACCCAGTCAGCCTTCT-TCCGGTGGGGCGCGGGGG-CAATGA + CTATCGTCGCCGCACTTATGACTGTC--TCTTTATCATGCAACTCGTAGGACA-G---CC + GGCAGCGCTCTGGGTCATTTTCGGCGAG----*****CC--T-T-CTGGAGCGCGACGAT + GATC-GGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGT-A + CTG-TCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTA-TCGCGGCATGG*CGGCC + GACGCGCT-GGGCTACGTCTTGCTG-CGTTC-CGA--CGAGGC-TGGATGGCCTTCCCCA + TTATGATTCTTCT-CGCTTC*-----GGCATCGGGAT---CCGCGTTGCAGGCCATGCTG + TCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGA*TCGCTCGCGGCTCTTAC + CAGCCTAACTTCGATCATTGGACCGCTGATCGTCACGGCGATTT*ATGCC-CC*TC-G-- + -GCA-CATGGAACGGGTTGGCATGGATTGTAG-CGCCG-CCTATACCTTGTCTGCCT--- + CCCG--TTGC-TCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGA---CGGC--C + ACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTG + TGAATGCGCAAACCAACCCTTGGCAGAACATATCCATC-CGTCCGCCATCTC---AGCA- + -CGCACGCG-------*CTC*GGGCAGCGTTGG*GTCCTGGCCACGGGTGCGCATGATCG + TGCTCCT*GTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAAT + GAATCACCGA-TACGCGAGCGAACGTGAAGCGACT-CTGCTGCAA-AAC-CTGCGACCTG + AGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTC + AGCGCTCTTCCGCTTCCTCGCTCA-ATG-ACCTGCGCTCGGTCGTTCGGCTGCGGCGAGC + GGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGG + AAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGGCCGTTGCTG + GCGTTTTTCCACTAGCCGCCCCCCT-ACGAGCATCACAAAAATCGACGCTCAAGTCAGAG + GT-GCGAAACCCGACAGGACTATAAAGATAC-AGGCGT-TC-CCCTGAAGCTCCCTCGTG + CGCTCTCCTGTTCCGA-CTCCTGCCGCTTACCGGATACCTGTCCGCCTTTTTCCTTCGGG + AAGCGTGGCGCTTTTCTTCAA-TACGTGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC + GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCTTGCGCCTTATCCGG + TAACTATCGTCTTGAGTTCCAACCCGGTAAGACACGACTTCATGCGCCA*CCTGGCAG-C + AGCCACTGGTA-ACAGGATTAGCAGAGCGAGGTATGTAGGC-GGTGCTACAGAGTTCTTG + AAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCCTCTGCTGA + AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAA-AC-A-AACCGCTG + GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAG + AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG + GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAAT + GAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCT + TAATCAGTGAGGCACCTATCTCAGCG-TCTGTCTATTTCGTTCATCCATAGTTGCCTGAC + TCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAA + TGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCG + GAAGGCCTGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATT + GTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTT-CC- + ATTGCT-CAGGCATC-GTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGG + TTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTA-TCC + TTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATG + GCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGT + GAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCG + GCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGA + AAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGAT*CCAGTTCGAT + GTAACCCACTCGTGCACCCAACTG-ATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTG + GGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAAT + GTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTC + TCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCA + CATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCT + ATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAATTCAA diff --git a/src/squirrel-1.4/seqs/puc18.vec b/src/squirrel-1.4/seqs/puc18.vec new file mode 100644 index 0000000..d7f9a6c --- /dev/null +++ b/src/squirrel-1.4/seqs/puc18.vec @@ -0,0 +1,47 @@ +<--puc.001---------> +;puc 18 vector sequence from vecbase library + GCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCA + CGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCT + CACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAAT + TGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGAATTCGAGCT + CGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGCACTGGCCGTCG + TTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCAC + ATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAAC + AGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGT + GCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGT + TAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCC + CGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTT + CACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGG + TTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGC + GCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGAC + AATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATT + TCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAG + AAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCG + AACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAA + TGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGC + AAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAG + TCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAA + CCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGC + TAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGG + AGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAA + CAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAA + TAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTG + GCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAG + CACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGG + CAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATT + GGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT + AATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAAC + GTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAG + ATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGG + TGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCA + GAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGA + ACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCA + GTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGC + AGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACA + CCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAA + AGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTC + CAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGC + GTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGG + CCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTAT + CCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCA + GCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGA diff --git a/src/squirrel-1.4/seqs/pwe15.seq b/src/squirrel-1.4/seqs/pwe15.seq new file mode 100644 index 0000000..0677190 --- /dev/null +++ b/src/squirrel-1.4/seqs/pwe15.seq @@ -0,0 +1,138 @@ +;cosmid vector pWE15 from embl entry pwe15a + ctatagtgagtcgtattacccgggcccgccggcgccacgcgtcgacggccggccgggccg + cggccgcgaattcttgaagacgaaagggcctcgtgatacgcctatttttataggttaatg + tcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaa + cccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataac + cctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtg + tcgcccttattcccttttttgcggcattttgcttcctgtttttgctcacccagaaacgct + ggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactgga + tctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgag + cacttttaaagttctgctatgtggcgcggtattatcccgtgttgacgccgggcaagagca + actcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacaga + aaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgag + tgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgc + ttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaa + tgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaacgtt + gcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactg + gatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtt + tattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggg + gccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactat + ggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaact + gtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaa + aaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagtt + ttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatccttt + ttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttg + tttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgca + gataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgt + agcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcga + taagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtc + gggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaact + gagatacctacagcgtgagctatgagaaagcgccacgcttccgaagggagaaaggcggac + aggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccaggggga + aacgcctggtatctttatagtcctgtcggggtttcgccacctctgacttgagcgtcgatt + tttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggccttttt + acggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctga + ttctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaac + gaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgctgacttccgcgtttcca + gactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcagacgttt + tgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaaccagtaa + ggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgcacccgtc + agatccagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtg + aaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataag + ctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcaggggga + ggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatga + tctctagtcaaggcactatacatcaaatattccttattaacccctttacaaattaaaaag + ctaaaggtacacaatttttgagcatagttattaatagcagacactctatgcctgtgtgga + gtaagaaaaaacagtatgttatgattataactgttatgcctacttataaaggttacagaa + tatttttccataattttcttgtatagcagtgcagctttttcctttgtggtgtaaatagca + aagcaagcaagagttctattactaaacacagcatgactcaaaaaacttagcaattctgaa + ggaaagtccttggggtcttctacctttctcttcttttttggaggagtagaatgttgagag + tcagcagtagcctcatcatcactagatggcatttcttctgagcaaaacaggttttcctca + ttaaaggcattccaccactgctcccattcatcagttccataggttggaatctaaaataca + caaacaattagaatcagtagtttaacacattatacacttaaaaattttatatttacctta + gagctttaaatctctgtaggtagtttgtccaattatgtcacaccacagaagtaaggttcc + ttcacaaagatccggaccaaagcggccatcgtgcctccccactcctgcagttcgggggca + tggatgcgcggatagccgctgctggtttcctggatgccgacggatttgcactgccggtag + aactcgcgaggtcgtccagcctcaggcagcagctgaaccaactcgcgaggggatcgagcc + cggggtgggcgaagaactccagcatgagatccccgcgctggaggatcatccagccggcgt + cccggaaaacgattccgaagcccaacctttcatagaaggcggcggtggaatcgaaatctc + gtgatggcaggttgggcgtcgcttggtcggtcatttcgaaccccagagtcccgctcagaa + gaactcgtcaagaaggcgatagaaggcgatgcgctgcgaatcgggagcggcgataccgta + aagcacgaggaagcggtcagcccattcgccgccaagctcttcagcaatatcacgggtagc + caacgctatgtcctgatagcggtccgccacacccagccggccacagtcgatgaatccaga + aaagcggccattttccaccatgatattcggcaagcaggcatcgccatgggtcacgacgag + atcctcgccgtcgggatgcgcgccttgagcctggcgaacagttcggctggcgcgagcccc + tgatgctcttcgtccagatcatcctgatcgacaagaccggcttccatccgagtacgtgct + cgctcgatgcgatgtttcgcttggtggtcgaatgggcaggtagccggatcaagcgtatgc + agccgccgcattgcatcagccatgatggatactttctcggcaggagcaaggtgagatgac + aggagatcctgccccggcacttcgcccaatagcagccagtcccttcccgcttcagtgaca + acgtcgagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagccgcgctgcc + tcgtcctgcagttcattcagggcaccggacaggtcggtcttgacaaaaagaaccgggcgc + ccctgcgctgacagccggaacacggcggcatcagagcagccgattgtctgttgtgcccag + tcatagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttgt + tcaatcatgcgaaacgatcctcatcctgtctcttgatcagatcttgatcccctgcgccat + cagatccttggcggcaagaaagccatccagtttactttgcagggcttcccaaccttacca + gagggcgccccagctggcaattccggttcgcttgctgtccataaaaccgcccagtctagc + tatcgccatgtaagcccactgcaagctacctgctttctctttgcgcttgcgttttccctt + gtccagatagcccagtagctgacattcatccggggtcagcaccgtttctgcggactggct + ttctacgtgttccgcttcctttagcagcccttgcgccctgagtgcttgcggcagcgtgaa + agctttttgcaaaagcctaggcctccaaaaaagcctcctcactacttctggaatagctca + gaggccgaggcggcctaaataaaaaaaattagtcagccatggggcggagaatgggcggaa + ctgggcggagttaggggcgggatgggcggagttaggggcgggactatggttgctgactaa + ttgagatgcatgctttgcatacttctgcctgctggggagcctggggactttccacacctg + gttgctgactaattgagatgcatgctttgcatacttctgcctgctggggagcctggggac + tttccacaccctaactgacacacattccacagccggatctgcaggacccaacgctgcccg + agatgcgccgcgtgcggctgctggagatggcggacgcgatggatatgttctgccaagggt + tggtttgcgcattcacagttctccgcaagaattgattggctccaattcttggagtggtga + atccgttagcgaggtgccgccggcttccattcaggtcgaggtggcccggctccatgcacc + gcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaaccc + gttccatgtgctcgccgaggcgcataaatcgccgtgacgatcagcggtccaatgatcgaa + gttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacc + tgcctggacagcatggcctgcaacgcggcatcccgatgccgccggaagcgagaagaatca + taatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgt + cgggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggacca + gtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatc + atcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacc + tgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccc + cgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctct + cccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcacc + gccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacgggc + ctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatctt + ccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgc + cggccacgatgcgtccggcgtagaggatcttggcagtcacagcatgcgcatatccatgct + tcgaccatgcgctcacaaagtaggtgaatgcgcaatgtagtacccacatcgtcatcgctt + tccactgctctcgcgaataaagatggaaaatcaatctcatggtaatagtccatgaaaatc + cttgtattcataaatcctccaggtagctatatgcaaattgaaacaaaagagatggtgatc + tttctaagagatgatggaatctcccttcagtatcccgatggtcaatgcgctggatatggg + atagatgggaatatgctgatttttatgggacagagttgcgaactgttcccaactaaaatc + attttgcacgatcagcgcactacgaactttacccacaaatagtcaggtaatgaatcctga + tataaagacaggttgataaatcagtcttctacgcgcatcgcacgcgcacaccgtagaaag + tctttcagttgtgagcctgggcaaaccgttaactttcggcggctttgctgtgcgacaggc + tcacgtctaaaaggaaataaatcatgggtcataaaattatcacgttgtccggcgcggcga + cggatgttctgtatgcgctgtttttccgtggcgcgttgctgtctggtgatctgccttcta + aatctggcacagccgaattgcgcgagcttggttttgctgaaaccagacacacagcaactg + aataccagaaagaaaatcactttacctttctgacatcagaagggcagaaatttgccgttg + aacacctggtcaatacgcgttttggtgagcagcaatattgcgcttcgatgacgcttggcg + ttgagattgatacctctgctgcacaaaaggcaatcgacgagctggaccagcgcattcgtg + acaccgtctccttcgaacttattcgcaatggagtgtcattcatcaaggacgccgctatcg + caaatggtgctatccacgcagcggcaatcgaaacacctcagccggtgaccaatatctaca + acatcagccttggtatccagcgtgatgagccagcgcagaacaaggtaaccgtcagtgccg + ataagttcaaagttaaacctggtgttgataccaacattgaaacgttgatcgaaaacgcgc + tgaaaaacgctgctgaatgtgcggcgctggatgtcacaaagcaaatggcagcagacaaga + aagcgatggatgaactggcttcctatgtccgcacggccatcatgatggaatgtttccccg + gtggtgttatctggcagcagtgccgtcgatagtatgcaattgataattattatcatttgc + gggtcctttccggcgatccgccttgttacggggcggcgacctcgcgggttttcgctattt + atgaaaattttccggtttaaggcgtttccgttcttcttcgtcataacttaatgtttttat + ttaaaataccctctgaaaagaaaggaaacgacaggtgctgaaagcgagctttttggcctc + tgtcgtttcctttctctgtttttgtccgtggaatgaacaatggaagtcaacaaaaagcag + ctggctgacattttcggtgcgagtatccgtaccattcagaactggcaggaacagggaatg + cccgttctgcgaggcggtggcaagggtaatgaggtgctttatgactctgccgccgtcata + aaatggtatgccgaaagggatgctgaaattgagaacgaaaagctgcgccgggaggttgaa + gaactgcggcaggccagcgaggcagatccacaggacgggtgtggtcgccatgatcgcgta + gtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggcaaagcggtcggac + agtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacg + ccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtacc + ggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagc + gcattgttagatttcatacacggtgcctgactgcgttagcaatttaactgtgataaacta + ccgcattaaagcttatcgatgataagcggtcaaacatgagaattcgcggccgcggcctcg + agggccgatcgcgagctcagtactccgcggatttaggtgacactataggatcc diff --git a/src/squirrel-1.4/seqs/sCos-1.seq b/src/squirrel-1.4/seqs/sCos-1.seq new file mode 100644 index 0000000..87755e0 --- /dev/null +++ b/src/squirrel-1.4/seqs/sCos-1.seq @@ -0,0 +1,159 @@ +GAATTCGCGGCCGCAATTAACCCTCACTAAAGGATCCCTATAGTGAGTCG +TATTATGCGGCCGCGAATTCTCATGTTTGACCGCTTATCATCGATAAGCT +CTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGACAAAAACAGAGAAA +GGAAACGACAGAGGCCAAAAAGCTCGCTTTCAGCACCTGTCGTTTCCTTT +CTTTTCAGAGGGTATTTTAAATAAAAACATTAAGTTATGACGAAGAAGAA +CGGAAACGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAG +GTCGCCGCCCCGTAACAAGGCGGATCGCCGGAAAGGACCCGCAAATGATA +ATAATTATCAATTGCATACTATCGACGGCACTGCTGCCAGATAACACCAC +CGGGGAAACATTCCATCATGATGGCCGTGCGGACATAGGAAGCCAGTTCA +TCCATCGCTTTCTTGTCTGCTGCCATTTGCTTTGTGACATCCAGCGCCGC +ACATTCAGCAGCGTTTTTCAGCGCGTTTTCGATCAACGTTTCAATGTTGG +TATCAACACCAGGTTTAACTTTGAACTTATCGGCACTGACGGTTACCTTG +TTCTGCGCTGGCTCATCACGCAGGATACCAAGGCTGATGTTGTAGATATT +GGTCACCGGCTGAGGGTTTTCGATTGCCGCTGCGTGGATAGCACCATTTG +CGATCAGGCGTCCTTGATGAATGACACTCCATTGCGAATAAGTTCGAAGG +AGACGGTGTCACGAATGCGCTGGTCCAGCTCGGTCGATTGCCTTTTGTGC +AGCAGAGGTATCAATCTCAACGCCAAGGCTCATCGAAGCGCAATATTGCT +GCTCACCAAAACGCGTATTGACCAGGTGTTCAACGGCAAATTTCTGCCCT +TCTGATGTCAGAAAGGCAAAGTGATTTTCTTTCTGGTATTCAGTTGCTGT +GTGTCGGTTTCAGCAAAACCAAGCTCGCGCAATTCGGCTGTGCAGATTTA +GAAGGCAGATCACCAGACAGCAACGGCCAACGGAAAACAGCGCATACAGA +ACATCCGTCGCCGCGCCGACAACGTGATAATTTTTATGACCCATGATTTA +TTTCCTTTTAGACGTGAGCCTGTCGCACAGCAAAGCCGCCGAAAGTTCCT +CGAAGCTAGCTTCAGACGTGTCTAGATACGTCTGCTTTTTGTTGACTTCC +ATTGTTCATTCCACGGACAAAAACAGAGAAAGGAAACGACAGAGGCCAAA +AAGCTCGCTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGAGGGTATTTTA +AATAAAAACATTAAGTTATGACGAAGAAGAACGGAAACGCCTTAAACCGG +AAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACAAG +GCGGATCGCCGGAAAGGACCCGCAAATGATAATAATTATCAATTGCATAC +TATCGACGGCACTGCTGCCAGATAACACCACCGGGGAAACATTCCATCAT +GATGGCCGTGCGGACATAGGAAGCCAGTTCATCCATCGCTTTCTTGTCTG +CTGCCATTTGCTTTGTGACATCCAGCGCCGCACATTCAGCAGCGTTTTTC +AGCGCGTTTTCGATCAACGTTTCAATGTTGGTATCAACACCAGGTTTAAC +TTTGAACTTATCGGCACTGACGGTTACCTTGTTCTGCGCTGGCTCATCAC +GCAGGATACCAAGGCTGATGTTGTAGATATTGGTCACCGGCTGAGGGTTT +TCGATTGCCGCTGCGTGGATAGCACCATTTGCGATCAGGCGTCCTTGATG +AATGACACTCCATTGCGAATAAGTTCGAAGGAGACGGTGTCACGAATGCG +CTGGTCCAGCTCGGTCGATTGCCTTTTGTGCAGCAGAGGTATCAATCTCA +ACGCCAAGGCTCATCGAAGCGCAATATTGCTGCTCACCAAAACGCGTATT +GACCAGGTGTTCAACGGCAAATTTCTGCCCTTCTGATGTCAGAAAGGCAA +AGTGATTTTCTTTCTGGTATTCAGTTGCTGTGTGTCGGTTTCAGCAAAAC +CAAGCTCGCGCAATTCGGCTGTGCAGATTTAGAAGGCAGATCACCAGACA +GCAACGGCCAACGGAAAACAGCGCATACAGAACATCCGTCGCCGCGCCGA +CAACGTGATAATTTTTATGACCCATGATTTATTTCCTTTTAGACGTGAGC +CTGTCGCACAGCAAAGCCGCCGAAAGTTCCTCGACCGATGCCCTTGAGAG +CCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTC +GCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCC +GGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGA +CGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCT +CAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGC +CATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGT +TCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCC +GGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGA +TGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCC +TAACTTCGATCATTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCG +GCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCT +TGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGA +CCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAA +TTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCT +TGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCG +CATCTCGGGCAGCGTTGGGTCCTGCAGATCCGGCTGTGGAATGTGTGTCA +GTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAA +GCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCC +CCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAT +AGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCG +CCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCC +GAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTT +TGGAGGCCTAGGCTTTTGCAAAAAGCTTCACGCTGCCGCAAGCACTCAGG +GCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGA +AACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGG +GAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATG +GCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGC +CAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGG +ATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGA +TCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGC +ACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGG +GCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGC +GCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGA +ATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGC +GTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTG +GCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTG +CTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCAT +ACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCAT +CGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATC +TGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTC +AAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGC +CTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCG +ACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCT +ACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCT +CGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATC +GCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCG +ACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCC +TTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGAT +GATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCGGGC +TCGATCCCCTCGCGAGTTGGTTCAGCTGCTGCCTGAGGCTGGACGACCTC +GCGGAGTTCTACCGGCAGTGCAAATCCGTCGGCATCCAGGAAACCAGCAG +CGGCTATCCGCGCATCCATGCCCCCGAACTGCAGGAGTGGGGAGGCACGA +TGGCCGCTTTGGTCCGGATCTTTGTGAAGGAACCTTACTTCTGTGGTGTG +ACATAATTGGACAAACTACCTACAGAGATTTAAAGCTCTAAGGTAAATAT +AAAATTTTTAAGTGTATAATGTGTTAAACTACTGATTCTAATTGTTTGTG +TATTTTAGATTCCAACCTATGGAACTGATGAATGGGAGCAGTGGTGGAAT +GCCTTTAATGAGGAAAACCTGTTTTGCTCAGAAGAAATGCCATCTAGTGA +TGATGAGGCTACTGCTGACTCTCAACATTCTACTCCTCCAAAAAAGAAGA +GAAAGGTAGAAGACCCCAAGGACTTTCCTTCAGAATTGCTAAGTTTTTTG +AGTCATGCTGTGTTTAGTAATAGAACTCTTGCTTGCTTTGCTATTTACAC +CACAAAGGAAAAAGCTGCACTGCTATACAAGAAAATTATGGAAAAATATT +CTGTAACCTTTATAAGTAGGCATAACAGTTATAATCATAACATACTGTTT +TTTCTTACTCCACACAGGCATAGAGTGTCTGCTATTAATAACTATGCTCA +AAAATTGTGTACCTTTAGCTTTTTAATTTGTAAAGGGGTTAATAAGGAAT +ATTTGATGTATAGTGCCTTGACTAGAGATCATAATCAGCCATACCACATT +TGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACC +TGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCT +TATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGC +ATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTAT +CTTATCATGTCTGGATCTGACGGGTGCGCATGATCGTGCTCCTGTCGTTG +AGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATC +ACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGA +CCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTG +GAAACGCGGAAGTCAGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGC +GCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTA +ATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGC +AAAAGGCCAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGC +GTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA +ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATAC +CAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCT +GCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC +TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGC +TCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGC +CTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT +CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTA +GGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAG +AAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA +AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGT +GGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCA +AGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA +ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACC +TAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATA +TGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTA +TCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTC +GTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGC +AATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAA +ACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCC +GCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTC +GCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGG +TGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGA +TCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTC +CTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCAC +TCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTA +AGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATA +GTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATA +CCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCT +TCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGAT +GTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCA +GCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGA +ATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATA +TTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTG +AATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGA +AAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTA +TAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAA diff --git a/src/squirrel-1.4/seqs/scchrIII.seq b/src/squirrel-1.4/seqs/scchrIII.seq new file mode 100644 index 0000000..59f7018 --- /dev/null +++ b/src/squirrel-1.4/seqs/scchrIII.seq @@ -0,0 +1,5256 @@ + cccacacaccacacccacaccacacccacacaccacacacaccacacccacacacccaca + ccacaccacacccacaccacacccacacacccacacccacacaccacacccacacacacc + acacccacacacacccacacccacacaccacacccacacacacaccacacccacacacac + cacaccacacccacaccacacccacacccacacaccacacccacacccacaccccacacc + cacacaccacacccacacacaccacacccacacacacccacaccacacccacacaccaca + cccacacacccacacccacacacaccacacccacaccacacccacacccacacacccaca + ccctaacactaccctaacactaccctattctaaccctgattttacctgtctcccaactta + ctctccattaccctacctctccactcgttaccctgtctgattcaaccgtaccactcccaa + ccaccatccatctctctacttactaccaccaacccaccgtccaccataaccgttaccctc + caactacccgtatccaactccactaccgcttaccctaccatcgaccatgtcctactcact + gtactgttgttcacccaccatattgaaacgtctacaaatgatcgtaaataatacacatat + acttatcctaccactctaatcccactaccacatgccatactcaccttcacttgtattctg + atcggtcatacgcacacggatgctacagtatataccatctcaaacttacctactttcata + ttcatcatcacccatctctcaccatcagtaccaatgcactcgcatcattatgcacggctt + gcctcagcggtctataccctgtgccatttacgcataacgcccatcattatccacatttta + atatctatatctcattcggcgacaccaaatattgtataactgcccttaatacatacgtta + taccacttttacaccatatactaaccactcaatttatacacacttatgtcaatataacca + caaaatcaccactaaaatcacctaaacataaaaatattctactcttcaacaataatacat + aaacacactcaattgcgtatctataccaccatgacgtcattaacgtaaaagttccttaat + attgcaaattgcttgaacggatgccatttcatgttgaatgtggtaacccaatagcatgat + atgagtaatgctttagtattgtttcagagttgtttcagtaatgttttagacaaggagaac + atatagtagcaaacctctaatccggtagtacttaagaaactacagtttctatgtacgaaa + gcagtaactatgtaattattacatttacatgacatataggaaggtccaataaacttacta + cattatgacctataagctagatcgtaattcattacgtcaacaggttatgagccctagagc + aatgcttctgagaacataatcaatctatctagccccaacaattataaacagtggctgtac + ggtatcgagaccgctgctgaatatgctaacgaatatatgaacgaattcgttcataccgga + gatatccaatcaatgaaaagggattacaatctcagcgcgaatgatgaaagctttgtcaaa + accgtatttaacagtttcctggtaaagctctacaagaaaactatcgtgggtgaagctgca + tgtgaaatgaactggatatgtgatgattcgcttggaagggtctctgcttatgatattttc + tcgcacttcgaagaaaactataatgaagtcactattggatccaggcttactcttatagag + gacctaccaaatatatcctccaagcctgtagatgaaattgcttcctttttgaaaactcta + ttcacgatgcttgaagacaatagcgaagaacaggacaaaaaaaaaagacgcgacaccaat + atcgcgttgttattaatgaccttcttacccgagttaaaagaatcattccacgagaaattc + ggtgactctaaggctcttcagctatcacaagtcattagattctgtaaattaaatgcgtca + tcgaattcatcatcttcggtctcagatgcattggttgcacaagacagaagaaactatcaa + aagaaaggaaataagggatgtatataatttacggagctgatcatcgcataagcaactgtt + ctctgcttaaacgaagaataccagaagcacgaatctttaaattatatcctaatgacaaaa + cgagtagatcttcatctgctagtgtcgcgattcctgactatgaaacgcaaggccaaacag + caggacagataacaccgaagtcctggctctgtatgttatcttcgactgttccagctacca + aatcctcagaatggatttttgacacaggatgtacttcccacatgtgccacgaccgttcca + ttttttcatcatttactagatcctctaggaaagactttgtcagaggagttggcggttcca + tacccatcatgggctccggaactgtaaacattggcactgttcaattacatgacgtatctt + acgttcctgatttaccagttaacctgatatccgtttgaaaactatgcactaaatcaaact + cttctgttacgttcacaaaagagggtgtcactgtgcaatcacctgatgatgtggtttcta + cagctgggtattcacaataaaagactctgggaagtacaaagctcgccttgtcgcccaagg + acatactcaaaaggctggtattgactatcaggaaacctttgcaccagtcattcgatatga + ctctgttagattatttctggcccttgctagctgcctcaaactaatagtatatcagatgga + cgttgacactgcgtttctaaactcaaccatggatgagccgatctacgtaaaacaaccacc + cgggtttgttaacgagaggaatcccgactatgtatgggaactatacggcggtatgtatgg + actcaaacaagccccattactatggaacgaacatatcaacaatactcttaaaaagattgg + tttctgtcgacatgaaggcgaacatggcttatattttcgttccacatctgatggtcccat + ctacattggcgtatacgtagacgacttacttgttgctgctccctctcctaaaatatatga + cagggttaagcaagaattaacgaaattatactcaatgaaggatctcggtaaagttgacaa + attcctcggtcttaacattcatcaatcgacaaacggagacatcactctctcccttcaaga + ctatattgctaaagctgcatctgaaagcgaaataaacacatttaaacttacacagactcc + gctctgcaactcaaagcctcttttcgaaacaacttccccgcatctaaaagacatcactcc + ttatcagagcatagttggtcagcttctcttttgtgcaaatactggtcgtccggacatatc + gtatccagtctcattactctccaggttccttcgagaacctcgcgcaatccatttggagtc + tgctcggcgggttctacggtacctatataccaccagaagtatgtgtctcaagtatcgctc + tggatctcaggtggcattaactgtatattgtgatgcatctcatggagctattcacgatct + cccacactctactggggggtacgtgactctacttgctggtgctcccgttacgtggtcatc + gaagaagctcaagggtgtgattcctgtaccatctactgaggcagaatacattactgcaag + tgaaactgtcatggagatataatggattcaaaacttgtttgaacacttaggccagccact + tatctcatcaacatcatatgtagataataaacctgctataaaactgtctaaacatcctgt + atttcacacgagagcaacacacattgctttgagataccacaagctaagaaatgcagtggc + agcaggcataattaccatagaacatgttatcacaaagaaacaagttgctgacatatttac + aaaaatccttccagctgaatcatttaaaacacatagggctgtcatgataagggaaccaga + aactacaaaataaccatactcatgcgtattcagttatggggggatgttaaatgtggtaac + ctaatagcatgatatgagtaatgctttagtattgtttcagagttgtttcagtaatgtttt + agacaaagaaaacatataatagtaaacctgtaatcaggtagtacttaagaaactatactt + tctgtgtacaaaacactaactatgtaattcttacatttacataacatgtagaaaggtcca + ataaacttactatattatgacatataagttagatcgtaattcactacgtcaacatatccc + acgataaaaaaaataccttgactcaagtgccgaaacaggaaataatgaaagatttcatac + aaggttgtttctaaggtgctatatgtccctacggcctcgtttagccccatctcgcatgca + ataccgtaacataggtatatacacacagagaagtagtaattttacgtgtcttatacttat + tttatatagaaatgtccgacacttgcgctacactaaggcatataagatagatcttaattc + cttgtattaacatataaacagacaacataacatagccagtttatcttacttatcttttcc + tggaagagatggcattatttgtgtgtatcaaagaggcctttttcaattttcattttaata + tttttttaatattcttatattgaacacatttttctcccctagatttatgtttcctattgc + tctccatatcacattatatctatgaatgaatatgcattgctcttctgatactatggtatg + agaggccttggtaaagggcatcgataaccgaaagaatcatccgcagagaagtagaatctt + agagcatatcgtctcgttgagaccgtatagattcgcaaaaataacagaaacttgcgttaa + cattgaatttcaaactcagttttccataattaactgttaaaaaaggctactataagggtg + tcaattactgttctgtttcgcaggattaattccgttatgtgctcttgtaattgagttagc + gcttcttatatctcttcagtaatgcagcagtaataaaacacattggcttcgctttcgctt + tttttccaaatcattatcgaagattctttgcttatgatatactggtgctcgaggatgatg + agaggacacaactttttgaggcttatcgttatcgtctgagtcacaacaacatacattaac + tgtccacgattcagggatgtgctatgagttcaattttcgctgtagtgctgccattggcgc + tgctactggtgtctggaaaaactccacagctttcaatggagatgtgactgcacgagtgag + acattatctgtattagcttcacaaccatactctgttagtaaggagccatagtcgacatct + gtccagctcaatcgggtatgatatttgatatccccaaaatacatggctgtaccatgtaaa + atgagcggtatatcgaagatcagtatatttcagcctatacaggatcctactatttcaaca + aaaatgatttcatcaaaattaaatcacgctgcaacattggtgaagaatctcaaaaggtta + gtaacatcaatatatcgcggagtagctatcattgtggcagttaacttaaaggatatacag + ttaattcaacaagattttcaagtccattttttttgggcacgctgaacacgctcaatgtag + tacttgttttggaaatcactcgcaagtttaatgggacgaattatatcagtaatgtgtaaa + ttccgacacaatgttcggttagtagatttactgctggattattgggttcacccgtagact + gttgccgactccaagaaaactatgatctcaatacaggcataaagaattgttgtatctgtg + cccatgtgatctgtaccttttttgcggtaccaaaatcgcatgttgcactcacactacgga + acacatcaacttgtaacgacaacaatacaaaataccaggagacatatcactttttttcgc + ctagaatgatttacaatggcatacacttagaaaagtgctatgacatgacgcccttactag + aagttacatgattctccttttttttctttctttctttctttttcggtacaacataattaa + tgcattcatacagagcgcatttccagcatcatttattggatagtaactcctcccgcactt + tcaatatcaatgacgctgactcttttactgacgctgagtctttaccgacgctgagtcttt + accgacgctgagtctttaccgacgctgagtctttaccgacgcttgtgaaaataaaaagtg + cgacacagctatatgcggcgcaagtacagatgtgggtctatcgaacatgtttatcctatc + ctgttatgtcatttttatcggaagtaataaaagtgtagaaatcacttgtttttgcctttt + ttgtgattttgctttcatttttttttgcccttttttcaccagcttggggatcaacatcct + aatttcttgttacttcgattatgtctaaaaagtgccttgatttgagagaaaatattttcc + tcatctgtcaagacacccacaccatcttcagtgaattcttcgtgtgtcgccttgtctatt + agttccgggtctctcatgaacattgtaaacgcaaagaacggtaccgtacatgccaagcca + accgtcattattattcgttgaacatatctgtaagattcaaccacagcatctctttcgggt + gagccccaaggataatcactaatgaatgtataaggtgatgcatatgctatttctgccaat + gtatcgtttccaaggttcttgtagagttggttaggcatggtttgtgtccatatggcacca + gaaacggaggcaccgatagcagcgctagctttgaaaaggtatactggatgccggttatta + cagccatcctactatgcgtagtcatggcttgcagtatgacgatcactgaattgctgcata + ggagaccactcaaacccatgataacagatgcagcgataacaccttcatgagacccagatc + ctcctctgtatttgtaaaaaaggcccatgcacaccatccatgcagcacaacctccgatta + cagaaagtttcagtttccttgtctttgccaccaacaaactgtagaatggagatgcagtag + cagcaacaaagtcaggaaggtttactatccgagcagccgaagtggacgattctttcatcg + ataccagcaaaacaggatacagatagtcacacgaaatgaaaaaggtgaaaaaattgaaaa + aagtaacaccaagggtgcccaaattccacgatcacttagtaatttgaacggtaggaagag + gagatttggcaaatttggctcccaatataaaaaaatgaaaaataaacagccacctgaaac + taaagtggcaattatttttgaattgtgccacttctgtgatgtctcattagccaacgtcaa + agggacaaggatacaccctagcgacacagttattaataatatgccaacaatatcgagttt + ccacaatagaaacaccaaattctcaaataatccgcccgttctttcctttctagcctgttc + tttgagagatctccactcagcagtctttgaagatttgtacttcatataaagaataagaaa + tataattggcaaggcagagagtgggtaaataaaagcccacattgcaatattcccagacca + gtttttctgaggatttgctgctgtgataatattacctgaaatccatggtattatgatata + tggccaatatgaggcgtactggtaaaacattctccacttcaaggaggagaaatcagaaag + tattaatgtcaggagcagatttgttccgacgtatccacagttatagaaaaccgatcctgc + tgcatacattgtgagacgggtcgcctgtgattgaatgatggttcccattatataaaaaat + agttgcaactaaaaaaagccttagtcttccgaagtggtcagagagtctggagtagacaac + ttgggatccgacacttacaacagcattgataacttggacagttgaaagtaaggagtgttc + tgaatatgagttcgtcgcatagcccgtataggtcgatctaagtgtgtagtctaaactaat + tccaaacccacatacaaacgcggtacttatcagtagaattttatatttcaaggaatcaaa + ctgtgcagacataatttcgttttctttgagcttaaaaaaggtcgatgtcatagagtacgt + gtcatcgtttgagtgacgttctcgctcaataatctcacaggactgcctaattccactttt + tttattacttgaggcaccaacaacactagaactcatccgataatttatttacttgattat + tccttttttttttttttctctttccagtccataaaactattttacttttcttatttatgt + aaatagtatttaagattctacggtcgctacatctgctagaaatggaattactttaataca + ttccatgttctcatatatacttttactgacacctttcgtcactattttttgtttaacttt + ttttttggaagtactaaaattatatgctcgtaattattgagtatcaaaaaaaaaaaaaaa + aaaaattgctgtgacaccccttcaatgtggtgtctatacacctggtgactttaactttaa + ctttgacctgtcgtcgtacaatcaacttagtttccgaaaatccacgacgaaccagttata + ccgaagacttttgtacgacaagaatactgtaccgtcgaaactatctcttttcaaactgat + gagtgaagcttgtgtttatgtctgttgaaacaaggaaaaaaatgcggtgcctgtcgacag + gatatgcgcacaagtgcaaaatcatcaaaaaattgagtacctagatccggtggtgtagga + cgagcaaagttccttcataaacaaaccgtaagggttactgatacacaatttcctttttgt + aaagagtatttgagcaaattagtcagcgattgaaaacaacacccttttttctatatatag + taggtgaaagttaacatgcgagtaaaagtagtgccacatttcctttgcagcaggtgcaaa + ttggtacatatttaacagatagcgccgcacctcatatgcagcgcgtacatgatacgagac + gacaagatatgcaaaagataatagtgtcatcacacctttatgagaagcgaattttttttt + ttttttggtttaaatatatatatatacatatatatagatatagatatacacatgtataga + tgtattctagttatgcttatacctagaactgatcagaccagatcatacctggtatagagc + ttaatcgatagaaattcaaaaatatggaggaaactaagtactcttcgcagcaggagatag + aagaagcatgtggttcagacgcttcattgaatgctagaggtagcaatgattctccaatgg + gactttccttgtacctctgcctggcttcgtgaactcttgtactattcataactgcactgg + atattttgatagtgggaactattattgacgtggtcgcagaacagttcggaaactactcca + aaacaggttggctcgttacaggctacagtttaccaaatgctattctgagtctcatttggg + gaagattcgcatctatcataggtttccagcatagtctcattttagcaatacttatttttg + aagccggatccctaattgctgcccttgcctcttcaatgaatatgctcattgtcggtagag + ttgttgctagtgttgggggaagcggacttcaaacgctttgctttgttattggttgtacga + tggttggtgaaaggtcacgtccattggtgatttccatcctaagttgtgcatttgctgtag + ctgctatcgttggtcctataatcggaggtgcctttacaacccatgttacctggaggtggt + gcttctatatcaatcttcctatcggtggtcttgccattattatgtttttactcacatata + aggccgagaataagggtatacttcaacaaattaaagatgctataggaacaatctcgagct + ttacttttagtaagttcagacaccaagttaattttaaaagacttatgaatggcataatct + tcaagtttgacttctttggttttgccctctgctctgcagggctggtccttttcctactgg + ggctaacctttggtggtaataaatatagttggaactctggccaagtcatcgcatatttgg + ttttgggtgtcttactttttattttttcattggtgtacgatttcttcttattcgataaat + tcaacccggaacctgataatatatcctacaggcctctccttctaagaagattggtagcaa + aaccagccataataataataaacatggtaacatttctattatgtaccggttacaatgggc + aaatgatatactctgtccagtttttccaacttatatttgcgtcgagtgcatggaaagccg + gtcttcacttgataccaatcgttattaccaacgttattgcggccattgcaagtggtgtga + ttaccaaaaagctcggtttagttaaaccactcttaatatttggaggcgttcttggggtaa + ttggagcagggcttatgacacttatgacaaatacgtccacgaagtcaactcaaattggtg + ttttgctattaccggggttttcccttggatttgctctacaagcatcgctcatgagtgcac + agcttcaaattaccaaagatcgtccagaagctgctatggactttattgaagtaacagctt + tcaatacattcatgaagtcattaggtacaactcttggtggtgtgctttcaaccactgttt + tttccgcctcctttcacaacaaagtatcacgagctcatctagagccttacgaaggaaaaa + cggttgatgacatgattttgtatcgtcttcaaaactacgacggttctcattcgactattg + gaaacattttaagcgactccattaagaacgtattttggatggatctagggttttatgcct + taggatttttgttttgtagtttttcatccaataagaaattaatcataccaaaaaaggacg + agacaccagaagataatttagaagacaagtagcgcagttattttcttattttcattttat + ttttttcgccttttatacagacttcaacacaatcagaatcaaataggtgtatcgcaatgg + aatgtaatttcttaagtattctatatgtacttaaaacctattaatatatggatcaacaca + gtatcttatgaatgggtttttgatttttttatgtttttttaaaacattaaagttttcggc + acggacttatttggaattcaaattattaatgaaagaacaattaactaattaatgtactta + gtatttggccattattatcgatttcgggggccaaatctaaccaaattcaacctacatttt + ttcaaattgattcaaacacctttcacaataagatttttatatctagcgcacatagaatga + aatgtaaacaaagatttcagaaaaatcgtcattcaaacttgtattagacgagggacggag + tgatttttgtgtttgtttttattaattgtgggataggatagtagcaactcttggaggaga + gcattgtcagttgtccagtctctgaagttaagtagtaagtttgcggagtcaaagggggat + ggcttttgccatttgtgagagttgtgcggcagcatcttattcaaatagagctgtattctg + aagacctcttgtagaacatcatccatactaaaaagtaaatcgtcctgtcccattacgagc + tgtagtagtgctgtgaccctctgtatatttacgttgccatgaagaaggtaatgggcgata + ttttgatacaattcctgagttgcatgttggattgagtttacgaagggtcgccagacggcc + agaaacctccaggcggagttaacaactagtaatacggcatccatgtttgcatcagcgccg + agcctataccagtcactgagtagacgttttcttgctctttttatgtcctgacttcttttg + acgagggggcattctctagagacacaggcagttgcttccagcaactgcggtacggccgtt + ctcatgctgtcgaggattttttttgggacgatattgtcattatagggcagtgtgtgactt + atgaattgttgtagaaggacgtctgtgatgttggagatatgtattttgttaactcttctt + gagacaatttggccctggatagcgaagcgtgcggttacaaataggtcgtcttgttcaaga + aggtaggcgaggacattatctatcagttacaaacatcttagtagtgtctgaggagagggt + tgattgtttatgtatttttgcgaaatatatatatatatattctacacagatatatacata + tttgtttttcgggctcattctttcttctttgccagaggctcaccgctcaagaggtccgct + aattctggagcgattgttattgttttttcttttcttcttctattcgaaacccagtttttg + atttgaatgcgagataaactggtattcttcattagattctctaggcccttggtatctaga + tatgggttctcgatgttctttgcaaaccaactttctagtattcggacattttcttttgta + aaccggtgtcctctgtaaggtttagtacttttgtttatcatatcttgagttaccacatta + aataccaacccatccgccgatttatttttctgtgtaagttgataattacttctatcgttt + tctatgctgcgcatttctttgagtaatacagtaatggtagtagtgagttgagatgttgtt + tgcaacaacttcttctcctcatcactaatcttacggtttttgttggccctagataagaat + cctaatatatcccttaattcaacttcttcttctgttgttacactctctggtaacttaggt + aaattacagcaaatagaaaagagctttttatttatgtctagtatgctggatttaaactca + tctgtgatttgtggatttaaaaggtctttaatgggtattttattcattttttcttgctta + tcttcctttttttcttgcccacttctaagctgatttcaatctctcctttatatatatttt + taagttccaacattttatgtttcaaaacattaatgatgtctgggttttgtttgggatgca + atttattgcttcccaatgtagaaaagtacatcatatgaaacaacttaaactcttaactac + ttcttttaaccttcactttttatgaaatgtatcaaccatatataataacttaatagacga + cattcacaatatgtttacttcgaagcctgctttcaaaattaagaacaaagcatccaaatc + atacagaaacacagcggtttcaaaaaagctgaaagaaaaacgtctagctgagcatgtgag + gccaagctgcttcaatattattcgaccactcaagaaagatatccagattcctgttccttc + ctctcgatttttaaataaaatccaaattcacaggatagcgtctggaagtcaaaatactca + gtttcgacagttcaataagacatctataaaatcttcaaagaaatatttaaactcatttat + ggcttttagagcatattactcacagtttggctccggtgtaaaacaaaatgtcttgtcttc + tctgctcgctgaagaatggcacgcggacaaaatgcagcacggaatatgggactacttcgc + gcaacagtataattttataaaccctggttttggttttgtagagtggttgacgaataatta + tgctgaagtacgtggtgacggatattgggaagatgtgtttgtacatttggccttatagag + tgtggtcgtgccggaggttgtttatctttcgagtactgaatgttgtcagtatagctatcc + tatttgaaactccccatcgtcttgctcttgttcccaatgtttgtttatacactcatatgg + ctatacccttatctacttgcctcttttgtttatgtctatgtatttgtataaaatatgata + ttactcagactcaagcaaacaatcaaagaaatctttcactgctcttttctgtgttccatt + tagtttttagtacgattgcattgtctatatactgtatttaccaaatcttaattttagtca + aacattaatgtttactttcgtatttaaggatgtgtatactaagccttgggaaaatgcgtg + ttccttgtgccatccgaaagctacttcataaaaaagatactgttcattttcaagcccgca + tagttggatcaaatattaactatatatcaacatggtccaaagatcgtaagtttggtctaa + tgttgttttagcaaaaaaaaatactatacctttcattaacgtttattgaatttcttgaaa + aaaaaaccctaaattattataaaattatagcaggatagttcttagtggaagatattaagc + aaactattttctgaattgctatgcgaaatttctctaatgccagctgagtaactaactctc + atggtacataaaaatacgcatcttaaaaaatttgatatttcatgatttatttatagaaat + aacgatgcttattgtgctttgttgggtgtttgacttctatgttaacttacttcaacatga + aagcccgacgtttgcaatgatatcctctttttcacccgattattttcatttattagtaag + tggggttctgggtgaaaaaatcaaaacaaaaatatgaatttgtaatgatttttatatttt + cgccggaggtgctggaaatggcaaacgaaaatactatgacataaaagctgggcacactat + gtacgttctttttaattttttatcaacatgagaaaaattcatgaacactgtaccttatat + tatctaataaatgaagtaagctttgcatctccgtaacagctgcatatatataattaagcg + ggagctttctttcgatctctttcattccatatgtttattatgtactgatgaaaagcattt + tactaagttgaggtctctgtaagtatattgtgaccattatgctaaaatactggggtcttc + atttgtgtcaaattctacgtaatagttcccctttttcttacatttttatactaaagagtt + acggtatttatacaaacgaggggctttactgtaatatatgattaagattcaagctgaaac + attctaagttagaggtataaaatgattattgatgaaagagctgcaaatgtcggcaaaata + taaataccttagatgttaccagctgggaagattattacggaacacattcttataaatcta + taggaatacataggattgtatttctattgacaaagtacaacctatattttatgttatgtt + atgttatgtttgctatgactatagttgcttttcttcacttagtaaggattaactaactct + cgagctagtaaatttgacgtgagaagcataattatattactaatagaacgatatctggtg + tgttttattacagactgcactgagtgttagaggtagaaattataagttaaaaagcagtag + tttatgctttatgctcgagtatcaagtgaatttgaacaggctagtgcttcattggtactt + ctttcatggataattttgagcaaatttctgcagcatgtccccctttatacaaattctgtg + cattgccggcctagaaatatgtcaacgttttggatatgttgatgcttacttcgagaaatc + ttacactaatacttctggaaaaaatcaatactagcaaaatagtgatatatgagtaaaatg + tatgtagtacatgtatgaaaattatcaagggcaaattgatgcttcaacgaaaaagttatt + ggattttcaagcactttttaaattcacaatatttttttctggaaatatgaaattgtcagc + gacttctattacaggagtgtctttttttctcatgctatccaacgcttcttccaagtcctt + tatagtattagaggagctcgcacaagcaataattatcacaatgtcatccgcagctaattt + tgagcccagtgcattttctaggatcttagtgttgtaacccaaatgcaatgcggcgccaca + tgccggttcaatcaccatattgaattgatgtgtatatttaagacagggttcaataacatc + tttgtcctctattacaacggatctggtgttgtatttgcgagcgtattcgaaagtttgatt + agagatcacggccgttcctagagaagtagcaatacttgttatcttcttgaattgaactgg + ttggcctattttcaaagaagtattgaaaacatgacatccattcgtttccacccccacaat + agggatcctatcagctaaaccatacctttccaaaccttgaataataccattgtataaacc + acctccaccaacgctgcatactatgcctttaaccttattcacggaaatatgttgcgattt + caaatcttgtactatttcatctatcatagatgaatgtccttcccaaatatccggattatc + gaagggatgaacataaatgggctcaatgacctgagagtctattttattcatgacatttgt + ttttaaaaaagtatctgcttctttccagtaggcaccactcacgataacctgggcaccggt + gttcctgattttatctaccattctcttctttgtcgctgtaggaaccacgactgtacatgg + tagagacagtctttgacatgctgttgcagcagcaaaaccggcattaccgccagaactagc + gaaaacctgaggagatcttttaccgtccttttgaattcgaatggcacttttcatgatgag + attaccgattcctctacttttgaaggagccacttggttgaaggcattcatatttcaagaa + aaattgtgcagaagcctttccggggaagaattgacgtaataatggtgttttattgtagac + tatcgacatctcgctggttaattttcctgtctcttgtctatccagcacttaaaaaagaga + aaactgtataaacattttccttttatttggcgatatttatatacctgtgatcctcctcat + attgtccctttaatcttatctcatcgcaaacaatttgataaagcttggcactattatgac + actcatggaattacatttccgctgggacttaacaggagcgcccatgcggaatcatgtctc + cgcgcagaggactcattagtttccgttcgcggtgattagtactcgcgaaaagagaaaaag + aaaaatagtaggaacatttcgcaatgatcaaaatgtaaacagtgcagtaaagaatgggac + cataagatagataagaaacagctcgcataaggaaacaaggacacatcgattaatggcaac + ccaagccctagaggatatcacggagaggcttttaataaggtcgcaagaggctatcttgca + attagatctgtggatacaacgtcagcagagatcatcaatatgccagacaacagatcaaga + gtcattggataagttatcccaacagtacaaccagtatatgtctcaactgaactctttgta + tgttagatcggaatctgttcgagacaagttgagcaaggaacaacaacgcagattgatcac + agaggataatgagcatcaacgcatagaagacttggttcgtgaattccaggatatcacttt + gaggttgaacgagctggccaccgtccaaatgaagcgcctaatgattctccacaatcgcaa + tccaccagaagtagtttagggtcatttcaacctcgaccattgaaaataattgagaggcaa + cgtctgtgtatggtaactccatcgaaaccaccaaaaaaatcggtaggctttaaccccatc + aatgaagtcgattgtccttcgaaaactaactctttaccgtgctcacctaaaaaacaacct + gcaagaaatcgactttacgtgcagccaaatcacatgatactggcttgaacaaaagtaaga + aaccgtcttcctcggatacgtatgagtctttcttcaaaaatagacaaagactttcgttga + ccttctttgatgaaatggatgatgaagattttgattctgatcaagatactatcattctac + caaacataagtacccctccacatgttggtgtgaccgcaaagggtgctgaattcgaacctt + taaggagatataactctcacgaaagtatactatctaacaaaccagcaccttctaagtcgc + tcaatctgggaagtttctccgcctcattcttcaggccatctaatccgacgtttggaactt + cgatatcaaatgtccaagtgaactgtcatccaactgttgcagcgacaatggctcctagtc + gtaatggtcctcgtatttcaagttctaaggcgttgttatcatcattcattgcacgatcag + atacacatacggtaaaagagaacaacacaaatcttaagcatgcatcttttatggataagt + ttaattcatcgttaagcacaatatcagaatcttttcaaagtaagagggggagaaagaata + agggcatgaatgaagaacgaatatcaaatcataatgtagcacaggaacaaaaaaataata + tggatataagcgtctctatagaagaattgcaagatgctttgaatacagaactgctgtttt + aattaatgcctcctttacctaacctactcttcttttgctccaaatgtttattcttttttt + ttttattcagaatatcttcaagtttttttgtatctttttgtattattattaaacttcttt + ataatatttgtcttataaaatcctatcataacatgactatggcttggcctagactcgggt + gccatcttttttaatgcgactacttcaagacagcttctggagttcaatcaacttcttcgg + aaaagataaaaaaccactaattatcaaagctatcttgtccgctttcaaaaagtttattcg + attttgttttcgttttcattttcatttttttattatgatggtcatggtggtagcggtggc + tcccttcagttttcctctttggtgctattaattttctcgttttccccatatatgtgatag + aagcttttgaactccccactgtcttgtaagactttagaatcttgactgtcttgttacctt + ctttaaacttatcatttatgtctgttctggacgcaaaggatttgattattgatggatgtt + tgaaaacgccaactagtgatgtatctccattttccacctcatcttcatcctctgggcgct + tttctaagtcaattattgtgtttactgtttttgaagtggtcgaatcagtttgagaatttg + tgaatgactttatcgaactattctgttttagggtatataaatcttcaattgcttcatcat + tgccatgttgtatccttgatagctccttatccgtttcaaaatcctcataattattacttt + ttaggaaggataagcttttctgcacaaagtcttcagatatgataacctttttgttctttt + gatctacaggtttctgttcattatttcccgtattatcacctacattaatgctattgtcct + gagatctaggtcagtagccgtacttgtaatgtctaggttatattcctcttcaggccccgg + aagggggtttttatattcgataatatcttccaccatactttcaaaaaatgcgctcgactt + tgggtttttaacaagctttgcatcatcgccaatttctaatctcctttttctcatcaattc + tctccttttgagacgatattgttgtagtacgtcgtcttcatcatcatcacttagttctag + ctctaagctattttttgctcttttattacgaaatcctccatttttaatgtcataaagaat + tttgtttatcattttgatatccatttccttattttctgcggcaagcatttctctgatttc + atgaggattgaaattgttcttggaataatcatcgatcattttttctaggtcagaatcata + gtcgtcagatccttctccatctgcaccaccgataccatgccattcatcctcggattcttc + ggcttccatttcaaagaagtttgtgacacctcttttcttcaactcttttatcttagcctc + gtgctccttttctttctgttttagttgcaatttctctttgtcaatcagctgacggcgaat + tgcttcatcagcttcttcattgacgtatacatcatctttgacttcattttcgattgtatt + tctttcggccatcacctcttcaatgttttgtgtatctagaacattggtttcttgaaattt + gtcggtatcctctccgatattatcaccgtaatggcccagattaatggcgatccttttcgg + tagactttcatcagctttctctttgggtttggcttcgacttcggtatcagagtctgattc + gttaatgatatgtttcacgtggtgggattttttttgctttagaattaatttcatcatctt + ccttttcactatcggattctctacgattgtcattttcactaccagatgattcataatcgg + caatttcattaccactcaattgcaaatccagaagactctgaccctgagtcagaaccggaa + tcatgggcgttcaattgaaagtcattttcttcaagtttttcccttctcttctctttttgt + ctgattcttttatttctaagaatttcttgttctagcaagttctctactatttccttttct + ttggccatatcctccagcttcagacctttcgtttcaatcacttctttctgatgatccaga + atttgtttcctgctagctttccttaaggtatttaatagtacattatgatcaacttttggg + tctttacttttattcggcctttgagacaacttttgattttgtttagagagtcttgccttc + aggtttaatatagtcgcctttgataactgagagattggcaaaacgctttcatctttggat + aatttaatgctatccatatcatcatctccatattcgtcactgtcgctgtcgaggtccagt + gttatgcatttcgaggagtcaatttcccgtttcaaattattagcataggagctcaatagt + ggaacccttttctcatttattcttgtctcatcagttagatttaattcaacacttttgttc + tcatgcagttcatcgtcattctgtactggttttggatgtgcattttccagctttatatca + tcgtcctcatttgaggacgaatcgtcaaaatcagcaagaaaagactctttagaaaaaaat + tttttggttggtattggcttttgcggttttttgtattcgacatttctagctttttccctt + ttagaatcttctgaagccaattcgctttgtatttcccgaatttttaatgtgtgtggtact + tcatcttgaagtgtttgtgcgactgtatctatttgggttttctgttcctgatgcgtagcc + tgtggaataccaattgcttgtgtcttatcctcatcatgcgcagtctgtggtggtacatcc + atttgggtgtcaggaccacgattttgaatggactgaaataaaggactagttccaataagt + tccgttggaattataggaattttcggcgacagagcattagaatgattgctggaagtcgta + attatttgggattgtggttcaattgattttataggctgagtttgtgaggttgcaccgctg + ctatctatacgctgtgtagccctggccgtcctgtcgtgagaggtgttttcagcgcttgag + aagtttcgttgacgagaccatcttctttgattaattgggtgggtttcccgtcattaacgg + aatggataggtacgttaaacaccttgtcttgttggatgctgacaggtattctttgcgtct + gcgtgaatgaagaagagacatttttctcactgtaactattatctttaacttcggatttct + ccagctcttcaccaccatcgtcaaagattagagattaattgagttggtaacgaattctca + ctcctatcttttccattattgtgattctgttcgggtgctttcttgccttctaatctgttc + ttaacacgattaatgtggcatttgcaaataaaaacccgttcccagtcaactctggcggat + tgtctatgtcattgggcccatttccattagtattatcgttctcatctagtatgggaacag + caacttttttgtaggtggtagttctcttctttgcagtcaacgaggacaaagcatgcaagg + catcatccatcactaaaatatttggtgataagttcaaaagcgaataacgaacttccttag + ttgtttgtctgttcttggtttttttccaacataatgtgtttgaaatgctattgaagcaaa + cgcgttttacgcattctatctcttcatgacgcgtcgaagaaggataaggaaaatttcaag + tattgatgtatatctctctgcatatgagagccagtagagttggtgtcctagtaagaattt + ctttcactttacaaacatatctaagtaaacgaaatgtgtgtgtgtttctatatatatata + gacatatatgaaggattccgtagcggtgtaaactaattttggtttggcttgtatgcttct + tcttccggagctatgaaatcttttgctctctcttcctgcctttcgatttctctttccatt + tgcttttccttttgctcatttaatttcttcatttgcttttctctcttgcttaagaaatac + tcaccactttcaatttccaaatcaacctttctaggcaattgggcaggaggaaatggagta + tagaccttcttttcgacgtttctgatcttcttgggtttctttctggccacattcctcttc + ttaaacatgggcaagaatcttgaccaatcttcattggctaactctggcctttttgccaat + tctctttttatcattaattccttgatatgatagatagggtgaatatttttcatacaatct + tctactactcgacggacctccttcaagcccttgaagggacccatggcacttactgtgtta + ccttgtactagaatgtaacatttagttagaagttccaaagcctttaaagtattaccgtta + gggcctacaagacgttgtcttctcttgacaaacctttctttgttagtaacgaaattacca + attttaataacgtcgcatgccatgtcatcttgtaggatcttaacggcttgcgggaaagga + acggatctcgccaataatttgatcaaatctctggctttcaaaatgatagcgggatcgtat + gtttttctagttgtttttactgtcatagaaccttcgactaaatctagaacacacgctatg + ttgtgtttgtctagagcccttgttacatcattccaaatcgtcttcaagtaactttctctg + tatttaggaaacaaagtcataaaactggactcttcagcaaaaggttgaccggatgcgtta + tcctcttccttaaactcctctatcttccatttatcaatatcatccgtatcccaaggttta + tctctgttatgtgtagacaccatcgtttgccaatttggatatttgtgtgacccttttgtt + tgctgtctactttacaatagttaacctcatcatctcttttttttgaaaattttcatatct + catcgctaaaagaattagaaatataaggaaaaaaaaattttcgttttcagatgtgcaagc + ctgctataataaggtacaataactcaagggcatttagcaaggaaaaaatgggcaagtgta + gcatgaaaaagaaaggtgtgggcaagaatgttggtgttggcaagaaagtacaaaaaaaga + ggtcgatcagcaccgctgaaaggaagagaacaaagttacaagtggaaaagttaaacaaaa + gtagtgaaacaatgataccgacgctgctgcgggaggcaagtacacaagagccagctaaac + tgaaagctgagactactttgaaagccgaggagctgatcaaggaccaggaaaaggactcca + aggtacgagagcaaattcggacagaaaaatcaaaaacaaacgacagcatgctgaagcaga + tcgaaatgatatccggcttttccttatagggaatagtggtgaaagttacgtaaatatata + catatagagcggaatggtactgcatatttttattatttttttttttgaatatatataaag + catcgtcgcttaagactaaaccttcgaggatctgaggccagcgctggatctaaagatggc + gtcgccctcggcgtagcctcttccaacaccaaatccaatgcctagccatacaggaaatgc + acggcgcttgaagaataatactgaggtgaacacacccacgccaaaacccatggcagtctt + gaccagcatgttggacaggacaatatcccacttagtgtcgaggatcgtggacacggagct + gccattcttgttagaatccttggagggagtgcttttcgctggttgttgtgtttgtgcttg + ttcggacatggtatattttgtctttttccgtgtttattccctctcgtagcaaagttaatc + tgccaattgacagtagtttaatatatggtattatcgttgtttgaagccctttaaaaggaa + aaaacgggttagggccacccggcgcgaagtaatagctgctgattggtcagaatagtaaaa + cggcttgaattgatagtatatcgtactgtatataccttatatttgggctaataatcatct + cccaaatgctccagtatacatgtcacctagctatgtatgaggttgactaccttatatgca + gctactacacaataactgtagtatcgcgctgcacgagcgtgacggcggttaaatctggat + tttttgccaccttttttatacgaaaataagaactaggtaaataggaaggaatggttggat + ttgagtttaaaaatagagggaatacttggagatgcgattgttgctgtgcaagaattggtt + tgcgtcacctgtaatctcaccactactgtatacccgctccttatattcaatggctaacac + tactagtttccctattgctccccaggccccgcctaattggtcgttcactcccagcgatat + tagtgggaaaaccaacgaaatcatcaacaacagcaacaatttctatgattctatgagtaa + ggtagagagcccttccgtgagtaattttgtggagcctttcatgaagtttgaaaatgaatt + gggcccaataattaaccaattaactttcttacagcatgtgtcgtctgataaagaaattag + ggacgcatctgtgaactcctcaatgaaactggatgagttgaacatcgatctatctctgcg + tcacgacatctttttgcaattcgcccgcgtctggcaggatgttcaatcgaaggcagattc + tgtggaaagagaaactttcaaatacgttgagaagtcttacaaggactacattcattctgg + tttggaacttgacgagggaaaccgattgaaaatcaaagagatcaaaaagaagatctccgt + taactctattaatttttcgaagaatctgggagaacaaaaggaatacatcactttcaccaa + agaacaattggaaggtgtgccggattctattttgacgcagttcgagacaataaaatctga + caaagatagcaatgaaaccttgtataaagtcaccttcaaatatccggacatttttcccgt + gatgaaattggcatcctcagctcagactagaaagcaggcctttttggccgaccaaaataa + ggtccctgaaaatgaagctatactgttggatacattgaagctgcgtgacgaattggcctc + gttattgggctatgacacgtatgcgaactacaacctgtatgataaaatggctgaagatag + cactacggtaatgaactttttgaatgatttgaaggacaagctaattccgctgggcagaaa + ggaactacaggtcttgcaagatatgaaagccgaagatgttaagaaacttaaccagggtgc + agatccaaactactacatttgggaccaccgttactacgataacaaatatttgttagaaaa + cttcaatgtggacctagaaaagatttctgaatattttccactagaggctacgattactgg + tatgctggaaatatacgaaacattgtttaatttgaagtttatcgagacgaaagattctca + aaacaaatctgtttggcatgacgacgtcaaacaaatcgccgtttggaatatggatgatcc + aaagtctccaaactttgttggttggatttatttcgatttacatcctcgtgatggtaaata + tggccacgctgccaattttggtttatcgtcatcattcatgattgatgacaccacaagatc + gtatccggttactgcgttggtttgcaatttctccaaatctacgaaggataaaccttctct + actgaagcataacgaaatagtgacctttttccatgaattgggccatggtatccatgacct + ggtgggacaaaacaaggaatcgaggtttaatggccccggatctgttccatgggattttgt + ggaggcaccttcccaaatgttagaattttggacttggaataagaatgaattaatcaacct + ctcatcacattacaaaacgggcgaaaaaattccagaatctttgatcaattcattgatcaa + aactaaacacgtaaatggtgctttattcactctaagacaattacattttgggttatttga + tatgaaagtacatacttgtaaagacttgcaaaacctgtcaatttgcgatacctggaacca + attgagacaggatatttctttgatttctaatggtggtacgttatccaagggttatgattc + atttggccatataatgtcagactcttactctgccggttattacggttatctatgggcgga + agtctttgcaactgatatgtatcacaccaaattcgctaaggatccgttaaatgccaagaa + tgggatacaataccgtgatattgtgttggctcgtggtggcctttatgatattaatgataa + tctgaaagaatttttgggtagggaaccttctaaggatgctttcttgaaggagctgggctt + acagaactaaattcgttcaagcataaaattatacaattattctacaaagtgttattatgt + tagataatcttttaaataacctcgtccctggatagattaaaaccattaaatagctcaaat + cttggagtaaagcagcagtaatatgtctgatatgttcgttatattttttcttgaagttta + tcaacttatcctttactagaccatttccctccccatccaatcgttgatcgattttaaatt + tcaccttgcataattttattaatttccatacacattttatcactgaaatgcacgagtaac + acagccaaactttccgtaatagcccacttacatataacttatcgttgaccacgcctaggt + tttccaatactgcgaagaaatcaagcagcgaacttacaccactccatatattctcccata + tatcttttgcactgatttcagccgtatttttcttcgaaaccattcaactttcttttcttc + ttcctttacttttattcatctcctctttacttcttacctcagagtcgaacattgaaatca + tttcccgcgaatttttcgattataattaccctttctacttaacaagaagcaataagagca + aaggggcgaaatgcttgtcccatactttggcttttggatgtgacattaggagccttatct + cctcttaatgccttaagtcaaaaaatagtttttgggatatatcttgtgatcaaatgcttt + gcaaaagggtaaaacagcgtttacaataggatagccaaaagtaaaaaaaaaaaaaaaaaa + aaaaagaaggaagttagtatcgagctcaaacaattttagttttgctggacatattttcag + tttcattactgtatatttgcagcttggtttttgtcgctctgtagtcttgtttcacacttc + gtcatttccgagtttcatcttgcgacataacttgtttcaacttttcaagtgcgacttttt + gttaaatattatgacatagaagaaagtagaacagccattataaaacaaaagagaagtgag + aatactattaagatggcattccaagatccaacttacgaccagaataaaagcagacacatc + aacaacagtcacttgcaagggccaaaccaggaaacaatagaaatgaaatctaaacacgta + tcattcaaaccctctagagacttccatacaaacgattactcgaataactacattcatggg + aagtcgctaccgcaacagcatgttactaatattgagaatagggttgatggctatccaaaa + cttcagaaattatttcaggcgaaagctaaacaaataaatcaatttgccactacgccattt + gggtgtaaaatcggaatagattccattgttccaacgttgaatcactggatacagaacgaa + aatttgactttcgacgtggtgatgattggctgcttaacagaaaatcagtttatttaccca + attttaacccaattgccattggatagattgatctccaaaccaggtttcctgttcatctgg + gccaattctcaaaaaatcaatgaacttactaaacttttgaataatgaaatatgggctaaa + aagtttagaagaagtgaagaattggtttttgttcctattgacaagaaatcaccgttttat + ccaggtttagatcaggacgatgaaacgttgatggaaaaaatgcaatggcactgttggatg + tgtatcacaggtacagtaaggaggtctacagatggacatcttattcattgtaacgtagac + actgacttgagtatcgaaacgaaggacaccactaatggtgctgtaccatcccatttgtat + cgtattgcagaaaacttctctaccgcgactagacgattacatattattcctgcaaggact + ggttacgagacacccgtcaaagtaagacctggctgggttatagtgagcccagatgttatg + ttggataacttctcacccaagagatataaagaagagatagctaatttaggttcgaatatc + ccattaaaaaatgagattgagctgttaagaccaagaagtccagtacaaaaagcacaataa + agcatcaacacataaatccttgcttagctcaattaaatatactagtaaataagtatataa + acaataattttgcattttattttactggaaactcttctcaaaaccaaattgcgcaaggat + tgattcagtacaattatgcaaactcgaaaagtaaataaacaaaaagatacagttgagtga + aaggtaaataaacattattctacttgattttcctctgttttttccatttttcttttttgt + cttttcgtgttctagcgtgttcactttttcagccatgtaattacttaaataggaagttta + cacgatgttctcgcataaaaagctggaagtgcagaacaaagaggaagaaaataggaacag + agaatttgtttgtttatttgtttgtttgcttgtttgtttgtttgttttgatttgaagtcg + aggtacttattcgaaataattattttgcccagggttttggatagttttttgttttccaat + tagatttttagaaatattttatcatagttttttgtttatttatttgtttatttatttgtt + gatttgtttatttatttgttggtttgtttatttaagtttgtaaatgtttaataaacaaaa + caatatttgcaagccattgaaaactttgtaaatttgtttgagaatttacgaaacaatgaa + aattaaggactctttgatggtgaacgaattactactttgcagcaggtaattctctaaaac + tcagaaacaaggcatgtatttttcactaggaaaaagaaggaaaatgggactacagtatta + gggaaacaggattaaaattcgtaggtaataataacccatactttcattgtagcagtactt + tcgttaataaaatggatattgttgtacttcggtagatgttatattctaaagagttaaatt + atccaattccaaattctagggacggtttccaaagatttgtattgtccagtgacataattt + tcactaaaacattgatctgtttaataggtttgctataaatgcacaataatttttcaccca + tgatacccaatttcaagagaagcaattgctacatataattatttaggctttactatctac + tactcattgactgtgcccttttacacaattataacaaatatgtcaaagcagatgccatga + actttgtatctgaatttttgatttccttttaattctaattgcagacgacgtaaatatagt + tctgaatttcaaagtcactgttaattaattgttctaattgtttggtttttttaatataaa + tcactagtgcttaagttctgttgacgcacacagtacctatctttgattccttcgtgcaaa + cagtattccggcacgttaattgataccaaaaagatttcaaaatcagatgcttcaagacta + attgtatactttatgtgacttctggagtttaatttgttgtattgatgagataatattgtc + tattatattgccaataacaaccagcgccaggtgtactaagtcgagatgacagaaaattta + taataagtgtaaatacaataaattttgtaaataaataactacttcaatagaaatctcaaa + gtcgaagaaacacagaacacagaagtaaactggactaaatgattttcaataaaggaaaag + cacgaaaaattttttgaacttttttgggacagcgtggatcgtgtaattactgtattggga + aatttacacgattctcgacaaataaagaaaaaggcgcgtttaaagacttacaagcacacc + tttgaattatttttgttctctatgattatcaaatgtgctagtgtttgcaagatggtgctt + ttttttctttagattgccacgtttctgctcctctttgtggttttctttttttttttcaat + cttacttataaaatgaatatttcaattgatgaatagctatatattggatacaaaaattag + catttaatcgaaaactgcagcatgtatttaatcgaaaactacagcatgtagctatgatac + gacagaagatttttgtttttatagttaagtcaagaagaaattctatttgtccagcaatcc + ggcgcaaagaagactactaaagggtattgaaaaatttctagaaccagaaataatcgaaag + cattactttttttggctctataaaggagttctggacagcttgaacttccgaagtctgtct + gtttagctcgttaagataaatctcaaaagagtagcaagcaggtgggagggataatttgga + ttaaagtgtatatattgcgtatatatagccacgtaaacctcacactattgagtttaaaca + tttttcaccttacgttattattcttttgtctaattttaatctcctccagtatactaaaac + cgcaaaagcgggttacccagcttgtttctttttgtctgaaaaattttttgcgatgcccat + tatgaaaaattggcaataatagtatagtagttagtttaagtttagattctttcaatactc + attctgcttcagtttgtagttagatttaactcaatagaggtgattggcaaaaatgggtaa + gacacaaaagaagaatagtaagggacgtttagatagatactattatctagcaaaggagaa + aggttatcgtgctcgttcatcctttaagattattcagattaatgaaaagtatggccactt + cttagaaaaatcgaaggttgttattgatctgtgtgctgctcctggttcatggtgtcaagt + tgcatccaaactctgtcctgtcaactccttaattattggtgttgatattgttccaatgaa + gccgatgcccaatgttataacttttcaaagtgatattacaacagaagattgtagatcaaa + attgaggggttatatgaagacttggaaagctgatacagtgttgcatgatggtgctcctaa + tgttggtttgggttgggttcaggatgctttcacccaatctcaattaaccttacaagcttt + gaagttggccgtcgaaaatttggtggtgaacggtacttttgttactaaaattttcagatc + caaggattataataaattgatttgggttttccaacaattgtttgagaaagttgaagccac + aaaaccacccgcatcaagaaatgtttctgcagaaatttttgtagtgtgtaaaggtttcaa + ggcaccaaagaggctggacccaagattactggatccaaaagaagtttttgaagaattgcc + agatgggcaacagaatatggagtccaagatttataatcccgaaaaaaaagttagaaaaag + acaaggttatgaggaaggtgataatttactgtatcatgaaacctcaattttggattttgt + caggacggaagacccaataagcatgcttggagaaatgaataagtttacaattgatgagaa + tgaccatgaatggaagatcttgaagaaattgaaacaaaccacagacgagttccgttcctg + tattgaagatttaaaagtcttgggtaaaaaggattttaaaatgatcctaagatggagaaa + gattgcaagggaaatcttgggcatcgaagttaaggacgacgctaaaacagaaattgaagt + agtaccgttaacagaagaggagcaaattgaaaaagatttgcaaggtttacaggagaagca + gcgtctaaatgtcaagcgcgaaagaagaaggaagaacgaaatgaagcaaaaggaactaca + gagaatgcaaatgaacatgataacccccactgatattggtattgaagccgcaagtttggg + taaagaatcgttgtttaatttgaaaactgcagaaaagactggtatcttgaacgacttggc + gaagggtaagaaaagaatgatttttacagacgatgaattagccaaagataatgatattta + cattgatgaaaacatcatgatcaaagataaggattctgccgctgatgcggacgatttaga + aagcgagttgaatgccatgtatagtgattataaaactagaaggtcggaaagggatgccaa + gtttagagctaagcaagcacgtggtggcgataatgaggaagaatggaccggttttaatga + gggaagtctagaaaagaaagaagaggaaggtaaagattatatcgaagacaatgacgatga + aggtgtcgaaggagactccgatgatgatgaagccatcaccaatttgattagcaaattgaa + gggacaagaaggtgatcacaaattgagtagtaaggcacgtatgattttcaatgatccgat + atttaataacgttgaacctgatttgccagtaaataccgtcaatgatggcataatgagttc + cgagtctgttggtgatatttctaaattaaataagaaaagaaaacatgaagaaatgcacca + gaaacaagatgaagcagattcttcggatgagagttcaagtgatgactccgatttcgaaat + tgtggccaacgataatgcatcagaagaattcgattctgattatgattcagaggaagaaaa + aaatcaaacaaagaaagaaaagcattccagagacattgatattgctactgttgaagccat + gactttggcacatcagttagcattgggtcagaaaaacaagcatgatcttgttgatgaagg + tttcaatagatacaccttccgtgacaccgaaaatttgccagattggtttttagaagatga + aaaggaacattcaaagataaataagccgattactaaggaggcagcgatggcaattaaaga + aaaataaaggcaatgaacgcccgtcctattaagaaggttgctgaagctaaggcaagaaag + aggatcgtgccgtagctcggttagaaagatcaagaagaaggcgggcttgattaacgatga + ttcagacaagacagagaaggacaaggctgaagaaatttctagattgatgcgtaaggttac + caaaaaaccaaagaccaagccaaaggttactttggttgttgcctcaggtaggaacaaagg + tttggcaggtagaccaaagggcgttaagggtaagtataagatggttgatggtgtcatgaa + gaatgaacaaagagccctaagacgtatcgcaaagaagcatcacaagaaaaagtagtaaca + aagagcattatttttccattcccttgtataatattgaaaataaaggaaaagaaagcaata + gatatattatgtatacatagaattaatcaaattatcatcaaaaaacttataggaaaccta + tcgttttgaattcagaaaatacgagtgcacagtagaacaatttttattcccgttttactg + atcttttcttcttgttgttaccaaataccttggagaaaatgtatatgatggagatgagta + agcatagtaacgtaccatttttgatcttggaataatcctttgtgtcaggccttggaattg + gtactagaagagttgtcgagttcaaatggcggaatgtagtatcgtctgtaaagagagatt + catatcctagaccttttttataaaatggattacgggaaactttattgtcacctgtgtcgc + aggctaatataacttctggatcaaatgaaacttctaatttatcccctttattattagaag + gctcaatatatctagtatgcaatgtcacttcattcattgtgccggctttcaattcaaaga + tactttcagaaccccatgccttatctcgaagagagtattctggtaattctaagtcgtctt + ctccaaaaagtagtaagggagaggattggaatttatcgataaataattctaacggcaatt + gcaggtgcattagatacatgcatttagggcgttcttcgaaatctgtgagatcaatcatga + tttttggatgcaaaccgataggttcattcagatatagtgatgtcgtagtcgtcgagtgat + tgtatgcgatatggccctgtttatacaataaggatgttttctgacatctttccattttac + catcgtccatcctccaattacaacgtattccaccaatatcgacatcactttcatcacttg + catccacaaagaatacacctacttcgagtttctcatctttcactcttgcaattgtaaatt + cttcaccctgtttcaacagacgatattcaacaatttggatttgtgtgggctccactgata + tatcatatgtaaaatcttctggattccatgacagattcaaatctacttcgggaggcaagt + actgctctatgtcaaacttctcactgtgaaatgaattgtagactgggttagtaataaacc + tttccggcacatccgaagaatttgagtaaactgaaaagccagcactcagttcattttcta + taacgcttaaacttctagccaagtccgctctgggcctccaagtgattctcgtaaaggatt + tatccaagcttccagtcctctctaatagccacctttgttgtaaaaccacaccagaacctc + cacgaacagtcaaatgagtgtcattttggcgcatatgattaccgatatcctcaggggcat + tgtagagtacagtcactctatgtcttgtcaccattactcagctagaaacgcacacttctt + gcgtttattctcctcccgatttgattatgattagcgtttccattatttgatcttttttat + cagggggatcttcaaagaaaggaactttttttaggaaaaaaaaataatcatcttttatta + catgtgtaatacttcaagcaatcataaacaagagttaagaaggcaagtctgctgtagtgt + gtcctccaatactcttgcacgacaaagttttagttatccccttctacgtgtattgttcct + ccgtggtcgattgtgttcaaagattgggagctcttttcgtgttttgcactaagagcatac + tgttaatccgcaaaggccactaaaacttacctgcatttgtctacttaaagatacattcca + actatttacattcatttttatttctaaaagagcattctgaaccctcggcaattatttcat + actccttctcagaataggaaaatgcccaatacgcatactcaacatgtgcaaatatcagag + ccaaatcctgtaaatactttgtctacaccatccaaaagaggtcaccgccatcgcagatcg + ctagcaatatcaggagattttgattttttgaaacagcctgcagcaattgtgaatttacca + cctccacaggcggctgaaaattgtccttcaactgccccaactgctgtatcaagtacatta + tcgccaatacgctacaatagatttccttgcaaaaccaatgaagacgctggaacgttagat + ttgcctgaaccaagattttatccgttatcaccaaagaacaatctgcaaacaccaagtcca + cgatttttcattagtgaagagccaagtttttcatcgccagttaaaggcgtcccagatgcc + attattaaccttgacgatgcgttgaagacaaggcctaggtcatttaaatcacatagaaga + tctgaatccgctcctcctgatttggaggttatggtagataagggcaattgtgcagccggt + tctaactctatgattaaagaagaagaggactccttaattgaaccagaatcgaaaaatgaa + tattatgagcaaaagcttccaacagcactattatccccactgcggccttccctttgtgta + tctgaacaggccattgatgtagatgattcagctctcaatgggtcaccgacccatcacaac + catgggatgcaaaacgccaatgcacggaattccaacacattcaattcgttgaagatcaaa + ggccaaaagcaaagatattatcattatacgaagcagctacctttgaccgtaggctgtgac + tcgcaatctccaaaagaacaaaggtcggctgcttcaatgacaatcaatcaggcaatgaca + ccttcttccctggcctataccccttctaaactagcatctactcccgcaacaccagtatcc + ttttatgacagcaatgcggacattaacttagaaagtgataattttccactaaaagataac + cctagatatgccaaggatggttatcctaaaaagtgcggcaattcacagcttaatcgtgtg + ctggatagcgataaaagacaggattttagtggagaatcgagaagaagaagatcgggcagt + cctatctcccacatgcaacaccgcaacctgattgataatatgaaaggtagacgaaacagt + aacacgataaactcaatcttcaactacaagagtcaacattatgaaatgccatatgatgat + atgatgaaaaatgaaaacattaatgcacagtccatgcccttttcagtcaacggtgtcaac + aatgaaaatagtatcggaggggttattacgagagcggacgatgcaccccttcaacactct + gtggtcaaatcctgtacgcctgatggcaaggaagaaatgaataggcttaaaagtaatgac + agtaatgaatattccaagtctgaagggcagatcagaaccaattcgcaactaagtaaggac + attctcatgggtgaaccaggtgatatggttgatctgtcctcttttgtcaacacgcagaga + aaagcctcaaatgaaactggtgacttagtctttagtttatcccaggatgatgacgcactg + aaaacgttccatgcgagcaatagcgcagcaacaagcaatgaaagctggtgtattagcgat + ggtgcgttaggaaagcaggcgcaggacagtgaagttaggaggaaagaaatcaaattagga + ctctttagacatattttcaaggaagtaatacaacaatattaataataagtaataaaaagg + tgaatcgtaaccttagcggcattgattgcatgtagttttaataataaagtgtagaataat + aaatataaaatttcaaagtcattagaaataaaaattgaatagacatatacatatagaaat + tacttctatcatttaaatacatatatatatacatacatgtatacgtgtatacttgtactt + gtgaaggactcaatagttgtattcgtttggtttttgaccagaagtgtttgggaaaccaca + ttccaataagatatcgttaatcaattcaggtttttcagtaatcttggagaaaacttcatc + atttttggttaagatcataccacaataacctgtggagttgaaacctatcttcatttcatc + agaaaaggccttcgaacgtggaacgacacagatccattccttggttaacataagattgta + ggatttctttagttctggattttcgttcaaccagtcctggaaaaaggtcaaagctctttg + caatatggagatgtaacacatagctaataggtcttcatcaacagtttcttcggactccgg + cattggcaagacaaaatgagcgaacgagactttagcatcttgcaaaggttcagtattgaa + agttggtaggaaatgttctttaccattacatagtctatcttggaaagtgacgaacttttc + aggcatttgcaaaatttgcaaatgtttgtggtccaatgaagaaccactggctggaccaga + attgtaaaagaccatgtgtctcttgtcggattcttcattgtccaaggcacacagcaactt + ataagcagtcaataaatcggttggggtcaaggcatcagtttgatgttggtattcgttagt + taccaataaagtgtgtccagggattacagggaatttgtttaatagcaatttatacgcttt + gttatcggcaccaccaaattctgggataaccgttaattcttcctcaggtttgcctagtgg + atcctctccttctggagtttggccacgctctggcttttcgatcagacttggcatgtggct + aatcaagtatggcatgctggtttttgggtcctttgttttcgttgtttcagtctggataaa + ttttaagttaccattatcgaaggcacttttgtacttgtcactaattaaagatgcaatgtc + agcggggatactcatttttattttaatgtttacttttctgtttgttctaaaatctatcta + aactggctttcaagatcaatctattgtcttttaaggtaaactttaaattggaaataatag + taatgttagttccttcattttaaccttgtattgtatttcctttgcgtgatgaaaaaaaaa + ctgaaaaagagaaaaataagaaaatcttctagaacgttccgaaacaggacacttagcaca + caaatacagaataggaaagtaaaaggcaatatatgaatgcagtgcttgtaactggtgctt + gtatccaagaatagcttcttgctgtaggttatgggattatcgtgtaagctggggtgactt + ttgagctattcgcgacgcccgacgccgtaataactactttcgacagaccacttatgacag + tatttcaggccgctcttataaaatgacatgttaacaaacagttctgattattcgccttct + gacaggacgataatgtaaatagttgtggtagtatcattcaggtatgtaactgtttacttg + gtatcgcttgaaaaaaataagcatttcagagccttctttggagctcaagtggattgaggc + cacagcaagaccggccagtttgaatgctcaactcttcaaaagaaattcctcaaatatgtc + cagtttcatgtactgtccggtgtgatttattattttttatttactttgtagttcttaaag + ctaaggtttttttctttcctaaattcttgtttaaatatcctaaaactaaagggaaaataa + acaatacataacaaaacatataaaaaccaacacaataaaagaaaggatcaaatactcatt + aaagtaacttacacggggggctaaaaacggagtttgatgaatattcacaagataaaaatc + atatgtatgtttctgatatatcgatatacaatcaaacactttcaagaatttgtttgtaga + ctttttgctagagacctcatcaaagtgctaccaactaagatcaacttatacttcttttag + agaaaatttttttcaatgtactccaaagagatttagatcctgtctcttcctcttcctctt + cctcgaaagtcaaagaaaaatcagagtctccctgcttattcaggcggagaggctctaggg + tagttgcgtttctctcattgggacactgaacctcattttccgacattttggtcatgtaag + aggcgacaggctcatcgcaggtaggtgcatcaacatggtagtacctggaccaagcgctac + attgagtccctcctggataaacaccgctacaatattgtctttgaacgtttgcccaaacca + tatattttgaataccaaagctggaccacattgtatggcttaatcattggtgctaccataa + tactggattgggaaacagtctggttaattttattcaaccaatttttcttatctagcaatg + atttaataaacctgaaatctaaattgtcttcgttagcgtctgtgtcataatctacaattg + agtactgtgacgtccaattatatggcaccgagatggggaatctgtccggtgtttcgtcgc + tgttatccttctcctccctccaaatgcagtcagaggcaggtgcccattcggttcgccagt + ctccgttatttactacttggtactgttcccaatcgtaatacgtttcctctgggttgaaga + tacttgctctgctcttgacattgcccatagccacaccacgagaaacatcgtggaagatta + cggagctgtttacgatagcaggagcaatggatttgacgaatgacacttgataaaagtctt + tggtcgaaaatacagtatcaacgccgtcgtgggactcgacccctggtagaagaagcagag + catctagatcaacggtagatgctgcagaggtcaaagccacaaagagggaagatgcagtta + cgaggtatttactaaacattggttcgaagtgattacagatttttctctcttgaaccctgg + aattgcaatagcttggttcctattgggaccggaccctcttgcgtaaagtatatatttaga + agcagctatttaaatctgaatgcaagacgaaaaaaagctcgtttataggagaaaaatacc + aaaaaaaggaaaaccatacacattcgggtatcccagctttgcttgcaaaatcaatcgatg + agcaagacaaacagggccagctgatgcatatgttttgtgttgctttcctacgatcagcta + atgcgagtgttataaatattcccctagttttcttctcctcctttatgtaacaataacttg + tggacttccccaacataagactaaaagcaaagggataacaagggatttatcttattccat + ccctttgcctagcgcactgttacgcagctgatcttatcaacaagtgtggttcttgcctct + aagatttacattgccttttcccaaagggaaggtgggacaaaactcattttccctaagggg + aaacgtaagataccctttagggaagataagggaagttagtagattgacgacgtaaatctg + tgatgagatgaaaaaaaaagattagcatctatataagaaagtacataacctccatttaag + ttgaaaaaaatttgtatgtaatgtcttttcttcctcttttctttatttcattctcaccgc + taaatcgataacggctaaaacaattaaagctcccttttcaaaccttatacttccgttaaa + taatgcaaatcaaaaacattgtcgctgttctagctacagtcactgctatcaacgcccaag + ttggcattgaacctaacgccactactccaaatgctacgcagccaaatgctacgcagccaa + atactactcttcctaccgcttcggtgactacgacagtttcgattggcgaagccgtagtta + ataccatggcggcgggtgcctttggagcagcaatcgctgccggtgttgccttcttattct + aggaacatttttatttcctttatttttttctttctttctaattccataaccgttgttcaa + aattggggagctttttaactttcgttccattaagctttttctttgttctttacgtttgat + tctatttcaaccataatttattttttcgttatttaataattactaatatttaatactaga + tgtatatacgttgtgtgtatgcgctactatagtatagcttaactgttttattataccctt + tttttctctctttactgtacagaagatgcgtcactaattcaaaaaaaaaaaaaaaaaaaa + aaatataaaaaagaaagattttggagaagctatatccttttgtgacatacaatagagaaa + cttgttctgttggtaagatatctcatctgaaatacaacaaggaaaaccaaaagccatttt + ttcattacttgatcgacttatgaaccgtattactaggaaaagttgtttattcgcgattat + atttgcatcattatttgtgacacatgcattgggtgccgctattgatccgccaaggcgacc + acataatgtgaagccttttcataacggtaatctcgaacttcaaagaagagcaaatgaacc + gttttttgaaatagatgtcaagagtctgaacacaaactcaccgatatcagagttgtgtaa + aaaagatttgcacgtcattgaatcgtctcatgatctttttcatttacaaaaccaatgtga + attcatcttggggtcattaaaagtcacaaactatgattctaacattttggatttgaacag + cttgagggccattggtggtgacctgattattcaggattcacctgaactgatcagaatcca + agccgggaacttgaataaaatcgaagggctcttccaattacagggactaacctctttggt + ttctgttgaaattccaactttgaaattttgtcagtcactggagtggaaagttgttcccat + cttgaactacgtctccatggattctcagaatattgagattataaaggatattgtcatatc + ggatacttcattagcaaacatcgagaatttcaacaaggttcaggaaattgatactttcaa + tatcaataataacagatttttagaaactattcattcgaacgttaaaaccattaggggaca + attcagtgtacatgcgaacgctaaggagctagaacttgaaatgccacacttgagagaagt + ggaaaacataacgattagggacacatcattggtctaccttccacaattaacaaaagtgaa + aagctctttagagttcatcgaaaattacttttacgaattgaacctgaacaatttgcagaa + gattggtggaacattaggaattatcaacaatgtaaatttaataaaagttaatttggagaa + cttaacagacattcaaggtggcttgatgatcgccgataacgaatccctcgaggatattac + tttcctgccaaacttgaagcagattggaggtgctattttctttgaaggttcgttcaaaga + tatcatgttcgatagcttgaaactggtgaaaggtagcgcttttattaagagttcatcaaa + cgtgttggattgcaataaatggacaaacccatcaaatggaagatcaatcatcaggggtgg + gaaattcacttgtatttctggtaagaaggaaaatacgctgaatgttaaacaggatggtac + aatcatagaaaaagggtacaaagatttaacgcaagaaggtgaagactccaagaaaagagt + gatttcaaaatacgcgaactcagcaaatccaagcatgcaattggacccccttctttttgg + tacatgccttgttgctatgttattgttttaagattactagtctagtataacagaaagttt + tttttgttcctaatattatctttatctatatacatatatctccatgaaaggcatttgaat + ttctatctttctctttcaatcaaatattgtcttattattgatcaaataatcgtatatgca + caatggcaactcgtctttcaaactctccacttgcccattcttcagtttgttcctaatctc + ggatgatgaaacatttcttaatgctggataatcattttgcataacatgaatcctagcgcc + ccattctcttggtattaccggttcgaattttccctcaaggatgtcactggcgtatcttag + ttgcgattcagcactgagatggcaatcgcctctcgccaagcaatataattgagatcccga + catgaagctctccattacatcactgatttttaaaggatgataatatttttcatcaaagat + cctagcaattgtatcaaaaccaactaagtagcctatatctgcactgcatcctttaacaaa + ttgctcatgtaatattttgtccttgtcgatgaatttcgagaaaacagtcaacccgacaga + tactacagattgggggagcttctcctgaaggtagtcagcgaataagcacatcatttccag + acgagttggaaaagatgccggcttaggcaacttatctgcattattcaccgctaacagtaa + taaaacatgcgaacgggtgtccttcaatttgaagtttttaatagtctgcgatagtagttg + aaaatgggccagatgtggtggattgaatgaagaatcaaggacaaataacttctgacattc + tagatgctggggtcctttgagaacctgaaatagtaaattgctttttcgaaactgctcgaa + cgtcttcttcatattagtctgctagtgcacgtactgctatcaaccgtcattgattaagtg + tccattttccaaagaagtaggagattatataaccacgagtatgacgtcttccagggaaaa + aaaaaaaaaaaaaaaagaaagccaaaataaggagccttgaaatgtgtatctgatatatat + aatgtgtatgtaaatatctatttaattagccattgggatttcaacttcttgtttgaaaca + gaaggacggataaaatacgtgatcaccaacaagacgaatatagtaataagcaatttaccc + ttttcgaaagtaggactcattaaatcaaattggcccgaaggtgtgatccttgtacaaaat + acatcaaggcctagatcacatataatgcttgtggattccagattggttgggatggagatc + aactgcgaatcggatcctggcaatagatttctgaaatgagtgataatgaaattatcatta + attgggataactggcgtgtatggggtagccataaactcttttttcttatccttggccatt + tcttctgctggtttacctcttgcattcaataaaagctttggtatgtaggtgatttgtcca + ttttctaattccataacgattgcctttgtggtaatatcatccgttgtcttggaaatggac + attgttttgataatctcgggaaaaatgaattgtttagtttgaaattgaggtttgttaatg + tgtccagtcaatggatcataagaaaaattgtcgcttgagttagacaaacgctcatctggg + gttagtgactcatataattccaccactactaacttttgttctggaacaggttcagaactg + aaataggaataaacgacccaatattcaccgaaaacaatatccattggaaacctaaaatcc + ggagaatccttgtgctcttgggtaatgaggatttctcctgtaatggtatcaattaagtta + aaagtgattgtatgatgttcttcattagcgatcagataagccgctaggttggggtacaaa + tatttataaagaaccgatttgtcacctagtgtaataccaagagtgtttaggtttgttgtt + tccctcttgctatatgccaccattttctctttcgaatttacggcttttttccaagtttgt + tttaccgtatcgttttcgattatgtagccataaatgccattcgtgtcatgctctgtgacg + aatatgtgggaactagaatggttgttggcaactattggtacatccgtattcttgccggga + ttcaatttgaacagtaaatgatccttatcctcaaacttaatcagatactggtgatcatgt + tcattcagcctaataacggaatcaacagttttcctttcagataggggggatcttgatttg + ataatcggttgatctttagtaacttcaattgtcaaataatgcccatcatgcgaaaaaaca + acaagttcgtcatggtttttatccgtccagaacattttgacgccctgatcaatttctaaa + tcgagtttccagataagttggcccttattgaccatatcaaggccgccgataaagccatca + tgcgtcaaaacaattaagattttggcgaatccgaacttcaaatctgataccgtggtatcc + ttagcgtctagagctaggagtttagtgaagacacgtcctggtgagaattggttttctttc + aataagttgataaggcgattccaattagttgtcaatctaagccagtaagcattccaaagc + gaatttgaatcaagttccgctttcatatctttgttcaactccacatccctagaatcgaag + acgtccaatacagcatagtctaccacgttagtcaaccattcatctctttgccaaacgggg + atcaattctgtgccattggcagagaatcccatatattgggtatcattaacgttcaacact + aacgccaggggatcttcacgatgaaaatattccactttattgaacccttgaggtaactcc + aatttccattctgccagttttgactgctcatcgagaatatgtagttcattgttcttaata + gatacttgatcgtttaaatatgaggattgaggtaaaatggtcgtagaaggggcattatct + acgccttcctctagtaaaaaccaatcgtgtaaatcatatttctgccaatggttgcttgaa + gagttcacacaaaccattgcgttatggtcattactatccagttgaatctcatcaatggta + aagggtaaaacgtttctgaaaagaatctgtccggaagaaacgttaaacgaagaaactaag + caggaagtttcggtagggttcgataagatgagaaccctgttgcggtctcgagaatcaggg + atgactttctcccaaggacctaagttagccagttgccaatcagtgataaatgcatcatct + gaaaaaacggcttggacacaactcgtgtttaggaagagtaaaatgaagacgtacaccaag + tctgtacacgttatcttcattgctatgggggaaggggaggatgaaagtgttgatatgaat + gtaggtattagttattaatggagtgtatatatatatatgttattatatatttgcatatat + aatatgaaatcccagccatattttctctggtagccgtctgaaaaatcacggtgtacgaag + aaggatttaatatacgcacggtacaactaagcaatccgcaaagacctaatgtgtcttttc + attagtgagagccttgggggtaggccctggtaacggctggtccgtgctagtgggtgtctt + ggtatgggagggcatggtagttggtatgaatttgatgcttagcgaagtttctaaggccaa + agtcatccatggatcataaagcgatcttattgtatcgagatcaatatcttgcgggccttt + cgtgtcatggggcaaaatgacacctcgggagagtgtggtgctggatctcttctttactgg + tgcggaaatggaatctcccgaagatgctaagaaatgcagtcttttttccacattttgtag + atggagttttttggactcggaaagaccttcaaggccagtagaagaagactgcggattgtg + ggatatggaattgatgagttcaatggaattttctctaatgtttcttttattttgaaaaaa + ctcgaacatgtccaagtgaaattccatgcgattgaggtactcgttgaaagacatgggctt + gtattccaagggtccgaaccatttaataatgggattctgcgcctcaacttgcgctctagc + gagagcgacgctttgtttgacctcagggatatttgtcgagagaggagtaccgttcatagc + atccttgacttttattttggtggggtctatgccctcgtgataatatttgacaatattgtt + gaaaaacttcttgtcacgtacggatttcatttcgtcgcaccaggggtcgtatttaagaaa + gttcaggcgagtaagctccaacagtgactgagaaagtagcaaagagccaaacaaccccca + taagagctttctgacgctgacgaatcgagattggcgaggtatgatgggaggaggggcgag + cacaatgtcaccatgggtggtggagaagcggttcaaagttggaaagtcagcaatatcctt + tttgattttagtcttgccattgacaatcatttcactgggtagttcgccaaaggataaatc + cgagtgagtgccagtggcaatgcggcgcggtggcggtagtttgtagagccttctcagtct + ccaaagggcaaagaaacccactgcagattggatagtgattagagtgtatagtcccggtag + gttatcaggagcgggtacgagcggaccccacagttttaagcctaaacttggccttatgta + gaatttcttgatatcattatcatctttgtcgtggtcatcttgtgtgtgagtatggtcggt + ggaattgctattacctgatggaggagtaaatacagccatcttttattacggtaattggaa + aaggagagggaatggaggaggatgagataagtagtttcctaagcagtaaagcaagtgtcc + acatttaagtaaaagacgatgccatgcacttattattactacgtggtcaattttctcttt + ccctaaggcgttcgtgcagtgtgacgaatatagcgaacaactattgtgtttgaattttaa + cgtttatctttttatgatttttttaaaaaaacttcctagaaaatttcttatatatctcta + tttaatgaaaaaccaaagtgatcagaattacaattcatcgtgaatggcatcttcttcgtc + agccaattcagcgtcagcatcggcttcctcagcagctttttcctgggcttcttcgtacaa + ggccttaccgtcgacgtcgaagtgaccgttttccttgatgaagtcgaataaagagtccaa + ggatcttgaaccttggtacacaacagattcggacttcttaccacctgggtataagacgat + tgttgggtaaccttcaattacgacgcctctgacatcgttttcagtgtggtctagtttagc + aatcaaaacgtcggatgtggcgttggcgtaggtatcagctagttcttggtaagttggggc + caatctcttacagtgaccacaccatggggcatagtacaaaacaagaacgtccttctttgg + gtcgttgacgatttcgtcatggttcttaccgaccaattggaagacagaggaatcttggtt + ctcgaagatctcttgggacttcacgattggggaggcatcacctttcaagaagtccttaac + caaagattcaatagccttagactccaacacgatcttgtcgctcaattcgtcaaacgcctc + ttcagagagttgaggcaaaccgtacttcaagtcttcagtcatgtcgtggatggcaaatag + agggaattgttccttcatgttcaagttgccggcgtgtctgccgaattttctggcatcgat + gctaacaaagttcattagacctctgttctttttggccaactcggtaaagagaggcttgta + ttcttccaattcttcctcgtcattgtagaataagtaacccaaaggcaaaccgctttcgac + gtattgggcgaaaacggaaccgtcgatttcaccaaagtagggcaaggcttccacttgcaa + ccatttttcaaaaacatcagcgtcagcgatatcggctttcttaccgttgtatactacagg + ctcgtccatggcggagggcaagtaaatagaaagcttgaaatcatcgtctgcgttttcagc + ggagacaaagtcgtagtcgttgaagtgtttgttggccatggagtaaaaggtggcgttgaa + gtcggcgtcaatcttaccggattggacgataactggagtgacaaaagtctcgttagcaag + gtaagctggtagatcagcaacaacggcgacagccggttggctttgcttgatcatgaattg + gacaatggcctcggcagttctaggtccctcgtaatcgatcgagttgttaacatcgctgtt + tttgaaaatcttcaagcttgggaaccctggaatgttgtgttccatacacagatcctggtt + ttcagtacagtcgatctgggccaaggtaatgtttttctcaactaaagtctcggcggcttt + aacgtattcaggagccatgttcttacagtggccacaccatggagcaaaaaactccgcaag + caccaagtcgtgcgactgaatgtactcattgaaggagtcggtggccaacttaacgacagc + ggagtcttcaggggccacagcctcttgttgggcgaaaacagaggaggcgagcagcaggga + ggaccatgacaggacggcaccagcagaaaacttcataacgggatagatgtatgtttttct + ttatgtagagcttggcagctttaaaattaccaattagacatgctgcttgcaacaagaaaa + tgcacgcgtaacaaaatatatatatatatatatatatatatgtatgtccatacgggtttt + tcgttttgttgtggttgtaacagcacaacaaaatgctacacggtggcaaattggaaaaag + agactagtgagagaaaagggaaaaagaggcgccgcccgacagggtaacatattatcacgt + gcagcccaggataattttcaggacacgtgtttcgaaaggtttgtcgctccgaaaaaatca + aaaaaaacaaaaaacgggaaataacaataacgacaaaaatggaaaaaaaaaaaattttag + acgcggcgcttgcaccccgcattataagtggtgtgccgacggacggtcaaccctttcagg + gggccccctatcttgcgtggtgtcacaccacgctcaaacggtgggcactaatgaaaaggg + gcccatataaatatccgctatcaacagaacccccaacccccccatcagtgcccaactcag + cttccgtaaaccacaacaccaccactaatacaactctatcatacacaagatgtcattcga + cgacttacacaaagccactgagagagcggtcatccaggccgtggaccagatctgcgacga + tttcgaggttacccccgagaagctggacgaattaactgcttacttcatcgaacaaatgga + aaaaggtctagctccaccaaaggaaggccacacattggcctcggacaaaggtcttcctat + gattccggcgttcgtcaccgggtcacccaacgggacggagcgcggtgttttactagccgc + cgacctgggtggtaccaatttccgtatatgttctgttaacttgcatggagatcatacttt + ctccatggagcaaatgaagtccaagattcccgatgatttgctagacgatgagaacgtcac + atctgacgacctgtttgggtttctagcacgtcgtacactggcctttatgaagaagtatca + cccggacgagttggccaagggtaaagacgccaagcccatgaaactggggttcactttctc + ataccctgtagaccagacctctctaaactccgggacattgatccgttggaccaagggttt + ccgcatcgcggacaccgtcggaaaggatgtcgtgcaattgtaccaggagcaattaagcgc + tcagggtatgcctatgatcaaggttgttgcattaaccaacgacaccgtcggaacgtacct + atcgcattgctacacgtccgataacacggactcaatgacgtccggagaaatctcggagcc + ggtcatcggatgtattttcggtaccggtaccaatgggtgctatatggaggagatcaacaa + gatcacgaagttgccacaggagttgcgtgacaagttgataaaggagggtaagacacacat + gatcatcaatgtcgaatgggggtccttcgataatgagctcaagcacttgcctactactaa + gtatgacgtcgtaattgaccagaaactgtcaacgaacccgggatttcacttgtttgaaaa + acgtgtctcagggatgttcttgggtgaggtgttgcgtaacattttagtggacttgcactc + gcaaggcttgcttttgcaacagtacaggtccaaggaacaacttcctcgccacttgactac + acctttccagttgtcatccgaagtgctgtcgcatattgaaattgacgactcgacaggtct + acgtgaaacagagttgtcattattacagagtctcagactgcccaccactccaacagagcg + tgttcaaattcaaaaattggtgcgcgcgatttctaggagatctgcgtatttagccgccgt + gccgcttgccgcgatattgatcaagacaaatgctttgaacaagagatatcatggtgaagt + cgagatcggttgtgatggttccgttgtggaatactaccccggtttcagatctatgctgag + acacgccttagccttgtcacccttgggtgccgagggtgagaggaaggtgcacttgaagat + tgccaaggatggttccggagtgggtgccgccttgtgtgcgcttgtagcatgatcttttta + catttttttggtttgtgtacgtatcccaccgtacttaccatcttctctcctttatatata + tatatatatatgtatattttcagtgtatatacatacattcttatacaataccgtataaga + acgtatgtatgtatgtatgattttctcacattaatgaacaatatcatcggaaacgggcct + ttggtttttaagggaaaatgaagaacaataaatgtcttgctaggtcttcctttatcattt + tccaactcaaatatgaaaagcagctccaaggggcgtactacttcaactaataaaatgtca + cacactaataagatcgcatacgtgttgaacaatgacacggaggaaacagcctcgccctct + tccgttggttgttttgacaagaaacagctcactaaattactgatacatactctaaaggag + ctgggctacgactccgccgctaaccagttactgctagagagcggcggatatcaaaatgag + tctaaccacatccagactttcttcaaactcatcaagaccggccaattccatcttattaat + tggcagattgtttgctcgctgccccttgcccatagctctccgctaaggtccgaatggctt + caaaggctgctcattcccacgccgacgcccgccacgacttcacttttcgaccacatgctc + ctgcaactgcaatacctgcaacagctgatgagctctgtaaattcatctacttgttcggac + gcagagatcgccacgctcagaaattacgtagagatcatgattctagttaatagacaaata + ttcctcgaattcttccatccagtcacaaattccgcctctcacaagggccctcacactgca + ctgcccgtcctgtatttgcgcaaaatcctcaaaaacttcatcgagatatgggattccctg + ctggtgtctaacgatcagttcctaaatgaagaaaacatcttcaatccggaaacgaccttg + agagaactgtcgacgtacctgaccaacccaaaactaaccgcgcagttgaacctcgagcga + gaccatctgattgacgccatctccaaatatatcgatccgaacgaactcgttcccaagggt + cgtctcttgcatctcttgaaacaggccatcaagtatcaacaatcacaagacatttttaat + atcatcgatccggatgatgacgcctctttctcctctccaccccaccggatcaaccttttg + caggataacttctcacacgatctgactgtgacctttcaagaatggaagactatccaagac + accacagacgaaatttggtttttgacattttcgcccaatggcaagtatttggcttctgcg + acttccgagtcttcaagaggctacttcattactgtttatgatgtggaacaagacttcaaa + atttataagacttgcgtgagcttgtcacaatccgtattgtacctcatgttttctcctgat + agccggtatctggttgcttgccctttcagcgaagatgttaccatttacgacatgaatgcc + acctccctgcccgatgcgtctgccacagactcgttcctactatacccttctaccaggctc + tcgcccatggactcgtttaaactggacaccaccacgtacccagatgataccgaatcatca + gcatcatcttcctcgaggccggcaaacgcgaattctaatcaatcaagagtatggtgctgc + gatgccttccacactgcggaacgtgcgggctggatggtggttggatcgcccgacagagaa + gctattgtacactcactcaccacgaaagagtcactttttagcttaaaaggcaggacttgt + atcgcgttgggccacgatgaaaacatctctgggagaaaatcaatcgatcctgcaaaagtc + ctttacaaaccaacaagtagcaatggtaactggcaatatgtggaggacgacgaaaccttt + ccacgtgtgcacgacgtgaaaatcagctatgacgataaatacgtgcttttaatgacccac + cagggcgttatagatgtctacgatttcagtgggttcccctccaaagaggagctatctaag + caaacagtggatcccaaaaactttctgattccaaggatcgcaagattggacgtgggcaaa + aacatgacttgtatctcattaccgctaaataccacgcatcaaggcttccataggcaacaa + atatccgagtctcaacatttggtattagttagccttcaagataacgaattacaaatgtgg + gactataaagaaaatattctcatacaaaagtactttggacagaagcaacagcactttatt + atcagatcttgcttcgcctacggtaataagcttgtcatgagcggatcagaagatggtaaa + atttacatttgggatagaattagaggtaacctagtatctgttttatccggtcattccacg + gtaatgagtaattccacaaaacccatggggaaaaattgtaacgtagttgcatcaaaccct + gcagataaagaaatgtttgcttctggcggtgatgacggtaagataaaaatctggaaaatt + tcaagaaattaatactccaccttatatatatacgtaagcaaagttttatgtaacaaaaaa + aaaaaaagaactaatccaaccttctttcactttcaggtaaaacttgactgagttcttcag + cctctcttctacctctcttgacatccaaacagtttagcacaggcaatgatagcatcaaaa + gcaaaaagaagaaataaaacgaatagcgaatattatgcgttttatcggtaagcagtccaa + caaggaagggtcccaggatggacgaccccttatctgtgatactgaacatactaaaaaacg + tggattcttttcctggaggtacaatcaaactgaaaactgatcttgaaaccgcggacaggc + cacctagtgataatccataccaaatggccaataagaacatttcaaacttatgctttaaac + cgaacgcattgaagaaaaatccaagaataccataaaatggtatgaaacttgcccaaatga + taatgtacatcaaagtttgactagaagtccaccgaaactttgtggcaagaaattgtggaa + tcataaaggcacccagcattgcatttacaacggtcaaaacacttatcatgattaaattga + gggtactcatgtgcagttctgccttggagaacaaaaccgctgtagaatttatagttgtaa + tggaatcactaataataaaccacgcaataagaaaaatcatcacatctttcaatagtctgg + catgtttaaacgattcgaaaagcgaaacccaaccatatgaaaggtaattcttccagttta + gttgacccaaggcgtcttgctcaccgggatacggactgcgggcggatgctaatgtagaat + cgtcaactcttatcggtattgtcacatcgtcaatcaaccagatcatgggcagttgccaca + caaaccaccaaatcccaacgaaaagaacagccacttgaacatcctgcttactgccctttt + tagatgcgactaagaacatagatacaatctgaacaatgagggcacttgaataacctaaag + atgcacctctaccgctaataacagtagttaacgagtctaccttatcaggttcataagctc + cttgactttgacatttcaaagaatccttgacaaaaatgggcagaagagaattcccaacaa + cattgataacgccaaaacaaccattggccactatataaagcccagccagagaataaatct + gggtatcattcaattttgaaatcgcaacagtcgacaatgcgcccacaataccaaaccaaa + ccagaattctgcctttgaatttaacgctcccccagagatctactatccctgaaacggaaa + tgactattatagtttggaataaaacgctaagggaaaagacatataatgcaaaacttgaag + tatctacgaagatccgattgttgaacaaaccaagaacacacttatctgaatcactacccg + tttctgacaggcagggtatggagtgatcgtgaacttttacaccatttatactcgcaaatt + gctgcagtagtaagggaatatacgttgaaaccgcagaaacgacaaatggttcgctagaaa + atgagtaagcgtaccatcccttgatattgttttgggctttttttattcgatagttcgtta + ccgattcattcatatcatttatagttccatagctcatatctaatgtagagaatatgttat + gagcaaactattgcttataagggcaaaaaccgtggtaatataggtacagtttgaaatctt + tccttttgtttacactacgcatctttcatagctgaggtattttcgtttaggaaaataccg + ttttaaggaattaattttagcaatttcctaaaattgttgagcggcgctatcaatgccgca + caatacaacaattaataatctaagacacgttgcattatacatgacagaagctaaagcatg + gtcttagatgacaataaaaaggaatttgacagcacaaaagagaaaaaaaaaactatacat + aaaattttttacatatgaattgcttagagtaaaataggcatttttcatatcttcttccca + attattaaattagcaaaaaattgaataaaaaaagcaggtggtttaagaattgcgcaggga + gaagaaagaatatcatatctcatgatggtagccacttagacagagagaaaacgaagtgag + aagaggctagtatttttatatcttaaaaaaaaaaaaaaaacaaaaaaaaaaaaacataaa + tatttgtatcttattgcttatgatgataatgtacaatgaattcaaaacatgaaaattgtt + caaagtttttatttttattttctctttcgtattaaactgatggaacaaatgaaaaaaatg + gataaaaaaacaaaaaaaaaaaaaaaacaaaaaaaaaacagctttgctggaggatgatta + acttttaatttttttaattattaggtttttcaattttttatggttttttgtgtaaaatgc + aatgaacgaatccattttttattttgcaagtgtgagaggcctttataaggaagactcgga + ttcaccggcagcaaccggcgcagagtcttctttggtttcttgttcttgagaagcgagttc + agtggatgattcctcttcttgttgaggaacaggtggtagggatatcatgaattggtccaa + tgcatcgccgaccagttctttttcaatattaatttcagtttcaaaagtttctggtaacca + gtttgaccagctcttggaacgaacgaagtacttatccaatggagatggttctttggcttc + accggtaacgttatcaccttccttggcggccaaagtaccttctgcaacattgacggtagc + ggcttcgttagcgacaatttctctcaatgcggctaaaatcaggttagtgtcacctccgaa + ggacatgttaacaactctgtaaaacttagagattaaagacaatggagcaaaaccatcctt + ggagagtttggaccttaagtaattgtcgacggtcaagttttcttcgctgaaatagtattc + aatttgtctagcaatgttgttgatggccattaacactggttggactggataaaactgttg + ctgtaatttgaccataggttgttgaggatgttgttggttgtgatggaagtggttgtggta + tttagagcggttgttgttgttattacgagcgttgcgtccttggtaaggtttaaactgtgg + tgggaagccttggttctgaggcataccggcgttattactgttgtggaactttctcctttg + aggaccattgctgttatggtgatgactgtggtgatgattacggtggtgaggtggttgttg + ctgttgtgtagattgaccattttcttgagaggtagcgtcttttgaatcttcctcacctac + tgccgattcaaagccatctttcttcatttgttgttgcttcttagcattatgttggtgatg + cttctttttcctgttagcattactcgacgatgtcgacgatgctgcagttttgttgttttt + gctcttgttgctgttgccattacttgcaccattcttggaaccggatcttttggtgccaga + gacggtaatggaggccttgatgggaacccacttggtagcagtcgatgatttcggagtggg + tgttctgttcttcttctttcttgtggcatccaagtcttctattgagatagtagaaacagg + gatctcagtaggagcaagtttccatggggaagatgtgggcaatggtgccggcgtcaagtt + gacttgcttgctcttggagctctcttgttcttgcacttctgggactggggcagtagcagt + gtttgcggcggcggtttcagcagacatcgtgggcagagaagtgaaaagaagatgaaacac + gttagcaggtccagagtaatcctgatgttctattaccgatctaggatcccaaaagatctt + cataaaaccgaaaaagatgttaagaaatgtttacacttgtacttttctttctttgttctt + gacctaccagaagtgaaaaaaatttttcattgcgtgggtacatccgtgcaccatttctga + aaaatttgcgatgagcatccctcaaaatccgtattatccgctagtgttttgaggttcaac + aagacaacatataagaagggaagaaggaaaggaggcaatgagaacttgggtcttgctcga + gagtccagatccacatgcaggttcaaaaaatggtgagagataacagtaataacggtagcg + ataaaagcgtccattgggagaggaggaataataacggcgcaggcccccgttatcgttcca + gaagcggtaataccggtgctttggcaacaaaactaagtaatgggacgctctctgtcagag + gattagtgaaggaccgaacaggaagcggcaagatcgcgggctgtgtggaggcgtttctgg + atgccaggacccaattgaatacgccctgggaccgtgctaagtgcaattggctggaccaga + tagattactatgtacagttgagaaagaccgcgttttctaaggaattggaccaactaagga + agcccatgatcgatgcatatgtggcggagatgaggcagaagtttgatgcctcctatggac + aatccagggcgcaattggaagccaaactggcgcaggtggacagtgaatggcatatggtac + atggtgatgtgcatgcaaaactggaaaaactcgtggaagaacgccggtttttgaaaagat + taagcgacacgatcgtaccacccaggtccaaaagatcacagcggctgtctccattgacca + aagaggaccgagccaactgtatctgtccgcagcccaaaggaatgagcgacaccgcttggt + tcgaagccattcagaagaaaatgttaggaatgaatggtaccatcaagctcctagagacag + aacagaaactactggctgacgagaaaaacagcgtgaggaagacgttctggcccatggtgg + aagcacattcacgctcgaatgaatttgcttatctggagaaatgcatcaggctgatggcct + ctcagagagcaatatgcttttgtcttgatatagaggctttcgaaacaaaccagaacgtaa + tcaccgaaattgggatttcaatttatgaccccagggaaaatatggtgccgtcaatggttc + caattacaaagaattaccacctaattatcgaggagtccctggaacttagaaaccaaaaat + gggtctgtgactacaaggattgctacttattgggagaaagctatgttttgagcttgaaag + agtgcgtgcatttcattcaatcactaataaactattacttggtcccggtgaccgaagaag + acaagacatggtcaagggcatttgttggtcatcacgtgagcggggatcttaagtggctgg + agactattggtgtcaaattccctggcagagggtatgaaggccatctggaccatacgctgc + ttttggctgaaactcccggtgatctagacgtgttcatcttggacactgagcagttttaca + ggaaatcgtatggcgaaaagggcagcagtctgggcaagattctgcggttgttcgagatac + cgcatgcgtttctacacaatgccggtaacgatgcctactataccctgcatttgttcatga + agttttgcgatgttaatttcaggaaaataagcggcatggacgatgttcttaaagtaatgg + gccaagtaaaagtttggggagaacgagacgtacgagagcctaaagtggtgcccatgtcgt + atgccatctccatcgaggaggcagtcaaaaatcggacgtaccgcaagggcgtcaagagca + gtaggaaggaaagagtctgccaaacggaattcggtgggttaacgtatttcggaactgcta + aagacgccttcacaagcactcttccgacacactaaatatattcatcgatttttttcctgt + aaatacaaacacatgcttgtatacatatacatatacatatatataagctcattataaacc + tgtgtgcatggaaaaaactttgtctgcccttaatttgcaagaataggttctaacaattcc + tccaattcaccagtctccctcaattcctgcaagtcgtcgttgcctccaatatgtttacca + ttaatatagatgtttggcacggttctttggccattaatctcatataacgcagcctgaatg + tctgcgccttccttcatgtcattcaattgcaaaaccagaactttggacctgggaaccttt + aacttttcaaaaagcgtgtttagggctgcatggcagtatggacagtacgttttggatgcg + acgaagatctcgttttctgcaataaggtccttgacgtgcttgatagtttcttgagatacc + attttttttccttctgaggttttgtctatttgtataattatgcgaatgctcgtataagta + ctgcacgaagttctttattttatgctaaagtccctttttatattatataaacttttcatt + tattttatatatttactcctttcaagacaaaattcccacatcacgtgaatcaggaggcgc + aaggggggtagtaaggcagtagtagataaagaaaacaagcatcgcaatctggaaacgccg + ttgcggctgctcgttatctgtagacagctcacttgtgcatgaagttcatgttttatagat + gatggagccatacttcaaggtgataactatgaaactgttttgaccttgacgtgtaaagtg + atcaaggtaatagttaaactttatatatgttgtttatcaatgtggtatcctttctaagga + tgaagacaacacgattgtgacagtgtgagagagtgataaagcattggaggcaggcaaaac + aagaacatggggtttctttcggatcatccgcatacagctatcaccgagacgatctttcga + attgtctcttcaagggactatacgctagaagtggagttagctcccttgattcagcttatc + aaagcagatcacaacgattataactataccgtgaaccaagaagaactgcgcgagcactta + gaaagaaaataaagtatgggaaccggctgcagcagtccaggactctggacctcttggatc + tgttcatctcacaaggcgtgaagtttacagtcatgtacaatgatgacaagctgctgcaaa + ggctgagagggatggctacgaactctgaaaacagcgggtccggtgaaaagtatgagccta + ggattattaagaagtgcgcggcgtatgctatctcgtggctaaactatattacgcagaaca + accttgagaatgcgagggcgtattcaggtctttatcaattgggtcaaacagtcaaacaga + ggtactcgaagagcagccgttcccgccgtagcgcgaggctctggcggacggtctaacttc + atggatgatagcgccgacgacactttgtaccagtccaactcgctgactagtgccgataga + ttgtataggattccacagatcaacatgaacaaagaggcacccaggattcggctcataatc + agcgatgctctcgcgtctgctgtgtcgttacagaactcgctgatcgggctgcccaagggc + aagttctccacagacgatgaagaggccacgtccaagttcatccaggcaagggcgatcaga + aggaaagttcttagatatctgcaattggtcacggagggtgagtttctgggtagcttaatt + catgccaatgatgaactggtggctgcgttgactgcatacgacgaccgaagcgctcaggac + gatagcagcgatgaaagtgatcacggctcgtacgatgacggtatttatgatgaaaacgag + caggataacagcaggtatattgatagcgagtcctcagaggaagagagcttgtcttcttat + cagccgtccacgatctcaaaccctttcggtgatcataacaaaatttgaagaagtatgtac + gtatgcatacacacatatatatatatatatatatagtacatttaatccttcttgaggttt + aaagacgcactgttcacacagtgtctggtgtccttgggcaagtttagcaactgtttccag + ccttcaccttcaaacacatgtcccaagtgtccaccacaccttgcacaacatatctccacc + ctcgcaggcattaaagaattgtcacgatgatatgtgatggctccaggggatacctcttcg + tagaatgcgggccatccacaacgagcgtcgaacttggccttgctcgaatacaacggtctg + tcgcagttggcacaatggtagacaccggactcgttggtgtgtaaatacgcaccggtgttg + ggcctttcagtggccttatctctcagcaccatcagctgcaatggtgtcagggcatcgttc + cacttcacgtcattcgattcgtcactcattttcttgctcttattccagtactgcgtcagt + acaatgtttcttctccctggaaaagtcctgcgtacagttataacgtacagcctgctccac + ttattcattttttgatattgcttcctttctccctttatttaaactatcatcggcgacata + tttcccaataggcaaagctgaaaaatactatcgctaattataacaaagaagctaggtcga + aggactcgaaaagacagaggtcgtactagcagagatagcaaatcagatggaggacggtaa + acaggccatcaatgagggatcaaacgatgcttcgccggatctggacgtgaatggcacaat + attgatgaataatgaagacttttcccagtggtcggttgatgatgtgataacttggtgtat + atccacgctggaggtggaagaaaccgatccattatgtcagagactgcgagaaaatgatat + tgtaggagatcttttgccggaattgtgcttgcaagattgccaggacttgtgtgacggtga + tttgaataaggccataaaattcaagatactgatcaataagatgagagacagcaagttgga + gtggaaggacgacaagactcaagaggacatgataacggtactgaaaaacttgtacactac + tacatctgcgaaattgcaagaatttcaatcgcagtacacaaggctgaggatggatgtctt + ggacgtaatgaagaccagctcaagctcttctccgattaacacacatggagtgtccactac + ggtaccttcttcaaacaacacaattatacccagtagtgacggtgtgtctctttcacaaac + agactatttcgacacagttcataaccgacaatcaccgtcaaggagagaatccccggtaac + ggtatttaggcaacccagtctttcccactcaaaatctttgcacaaggatagcaaaaacaa + agtaccccaaatatctacaaaccaatctcacccatctgccgtttcaacagcgaacacacc + ggggccatcacctaacgaggcgttaaaacagttgcgtgcatctaaagaagactcctgcga + acggatcttgaaaaacgcaatgaaaagacataacttagcagatcaggattggagacaata + tgtcttggtcatttgctatggggatcaagagaggctgttagaattgaacgaaaagcctgt + gatcatattcaagaacttaaagcaacagggtttgcaccccgccattatgttaagaagaag + aggtgatttcgaagaagtagcaatgatgaacggaagtgacaatgtcacccccggtggaag + actctaatgtgcagttgtcatgcacatcatcatactaaacttacacgaataggataacat + gtatgctagcagaatatatatgaaagaaaaaaattattgatgcctttaaacttatactat + tatactatattatgttatattatattattagttttatagatatattgagatatgttgaat + atgatgacgaggatggtggtttaagtgtatggattgaattttctcttagctttcattacc + ttgattctttcttgatcttccttaaatttacttaacagttgattgatctcctgtttttta + cgttctctgacctgaaatctatagaaatctttctttgcctttttatctatatttgaaatt + ggcttggccttattttcatgtttggataatggatttttgtttaatattttctttcttatg + gaattcaatgacttggtgttctttcctacaactaatgtgaatccatcctcatccactata + gaactttgaacatcctcttgtgcttgagcttcacgctgttcaaaaattgccatatgtgta + tgaatatcttctttcaaataatcaatatccagtggtttgtagaagttaacaaaagttgtg + aatgatggagtcgtatatgtccattcaaatagttcatttggatgtttggcatgcaaattc + gagtattttttcaaagcattccagcagttatttatacttgcagcatcaacaaattttaat + agcgccgtgtttcttggtgtgtatctcttctcgttgacgtcagtggaggacatcagatcg + gaggtcaatgccgataaatctacttcatgtaatccaaattcatcgttatatagtagttcc + tctacatgcgatactgtatcgtatttcccacagagctgcccgacaaatttcttcatgtgc + tctatgttagataataatggaaggttgaccaaaaacaaacagtcagactcgttggaattt + gaactctggtgtcttttagcaaacatgaaatgcaacgaagcttcctggcttttgggtagt + gccttgtgatccggtaatttgaacggcaccactataaacccgttcttcatggcgctaatg + tcttcaatacccatcccgtaacactctatctgaggttgttatctgttacctagttcgttc + gccctaaatgcctcttgctttaccatcctctcaatgttctcttctttttcctgcttattt + cttaggtgaccaaaaagtgaaaaattttccaatttaacttacgtcgttcgaagtgatgac + aataaggatattcatttattaatcgctatttgatacccactcttgctactacctctcttt + taatccaaaattacaatttttacgttaccattcattacatagtgtatctctattcattca + agactgtattttgtttgatatatatatgtatatatacatattattttcgttagtgttcgg + tttccaagttagaaataatctactggaaatcctttgggatcaacccaagcttactcattc + tgattttcacagcgtttctgtgaccgtctagaccctccttcttggcaacgcacataacag + ctctaccgatgttttctaaaccttcaggggtaatgttttgggcagtgataaacttttgga + aggttgcagtgttggcaccactgtactgcctagcgtaaccataggttggtaatgtatggt + tagtaccacttgaatagtcaccgcacgattctggagtgtaagcacccacaaatacggacc + ctgcattgtcaaccaatttaacataatcgttagcattggcgatttgtagaatcaaatgtt + ctggtgcatattggttggacatttcaagggcttcttcgtaaccgtcacaaagaacgatcg + tactgtgagcaatacatttacgaacaatatccacacgtggcagttgtaaagcttgattgt + ggacagcatcttgaatctcttgaattttcttttcgctcaagttaacaccaacaaggataa + cttgggagtcaataccgtgttcagcttgcgatagcaaatcacttgcaacaaaatccacat + cggcatcttcatcggcaataaccaaaacttcacttgggccagctggcatatcaatggaac + atagagcttgagtgtcattttgaacatacattttggcggcagtcacaaattgattacctg + gacccaagatcttatccactttaggaatagtttctgtcccgtaagccatagcagcaacgg + cttgggcaccaccagctagaacaatcttggaagcgccaactttttctgcgacataaacaa + cttcgggtgaaactttaccatcagattttcttggtggagatgcaaacacaatctccttac + attgggcaacttgtgctggaacaccaagcattaatgcagtacttggtaaaatggcagtgc + caccagggatatacaaaccaactttttcaataggacgagggaatctggaacacaagacac + caggttgggtttcaacttcaagagtctctgttggcaattgagcagcatggaatttgcgga + cgttttcaattgaaaggtccaaagcttccttcatttcctcggttaaaccttcaaagtatt + cttctgggaatggagcattaagaacaggattggataattttacaccatcaaacttttctg + tgtactccaaaagggcagagttacctttgtctctaacattttcgatgatcggattgacta + aatgcataatttcagaagtcttttggattggtctcctcaaagccttctgcacaccaactt + tgtcggaagccttcaccacgtccaagtgaattggaccggtcagtttttcttcttcagcct + ttggttgtccaacaaactttggcttagcatcacctttccgtcttgtaaccttcagatgct + tcatattcagattattctcgacgtccttcaatgaaacatcgttggccactaatttggcca + gtgcaaagtagaacaaatcggcagcctcccaagaaagctccttcttaccctttgcctcag + tcagttcttcagcttcttccttgatcttggcatctaacaatgcagagtcgttgaatagtc + ttctagtataagattcctctggagcgtcctgtagcctttgttttagtaaagattctagcc + ccaccaaaccatgcttgaattcaccaaagcaagacatggtctccaagtggcaaaatccaa + cgttttcttgttcaacgataaactttaaggcatccgaatcacagtcagtagagatttgta + aaagcttttggccattgccagaagtttcacccttgatccagatttcattcctagaacgag + aataataaacgccacgacccaaatcgatggcctttgctatagatttcttcgaagaataca + ccaaccctagacaacgctcatattggtcgacaactagggtggtatataaaccgtcaggac + ggtctgtacgtacttcaccaagcacttctttggtcaacatatccttgcttaatttcttta + tggacacaattttatcttgcgagaatttttgttttaccatgaattgattggagaaaacac + cgttctcttccacaacaacacgctcctttggtacattcaattgttcaaccaagtgttcgg + ctgttttagcatcttggctagcaatgaacagagaagaaactccgttgttcaagaaggcaa + tgatttcatcatcgctgaatttaccacttggcaaggacaaagccaccaatggaacttctt + cctctttggagaactggagaatctcttcattactcaggctcgagccatccaaaagtacct + gaccaacaagtgaaacgtattccttcttactattccatgaggccagatcatcaattaacg + gtagaatcggcaaaaccattattcagaaaaaaaattttgtaaactattgtattactatta + cacagcgcagttgtgctatgatatttttatgtatgtacaacacacatcggaggtgaatat + aacgttccatatctattatatacacagtatactactgttcatagtcatatccttttctta + ccttctatatcgaatgactgataaaaaaacgtgagtcactgtgcatgggtttagcaatta + attaactaatttaccggagtcactattagagtcagttcgactgcctagaagaactgctgg + ttgtcaggattgtgatgggggcattctgctgtattatgacccatcgtatcgcaatgctca + caccactgttgtcttcctgccgtggtatcgactggtgcaggggggtcgaaaattggcaac + gattccacggctgtttgtgcttgagcctgttccaactgtttgattttttcattagcctct + tcaagttttttcgttaaggatgccacctcttccgatgaggaatcttgtggttttgtcaaa + aatagttccttgctcaaattttggtattctttactgagcgaatcgttatgcattttcaat + tgttcgcgttctttagcccactttgtcttgtgtaactcaaattggtcttctatgttgcgt + aattgttccagctgttttttcaggagttcgacatcttcgttggcaccagtgggttgatta + tgagaaagatttctctcttcgttttctttgatctcttcgtgtagttggcttacgacagca + agtagctgttcattctcagcgtcaaaaaactgcttttgtttggcttgctgtctgcgttcg + agctccaattgttgcttgagatggtctatctctttctctctttcttgtattgtggcttca + tacctatcaaaagtcggttgcacttcttcgaggaccattctttggtcatcgagtagcctt + ttgtagtgtagttgtttcctttgtagcttttcgatggtcaattgggcttctcgtaattca + attgtaacttcgctgctattgaggtcattcatgtggccattgtccggtttccaatcgctg + gtggtgttgtgattagcctttctgtctgatgacaggatagagtcgacctccattctgtct + tctctgttatcgtaaccaaattcttgctgttgatggtgatccgatgcctcctggtccatc + gactgttgattaccgctgtgccgactggtgatccggaaacttctcatgggtgtgggggat + ttaggatcatccatgggagagaactggttagtgagcctcacaatagatctgtttttgggt + attgatagcggttccatcgtcgttcttctcgaggtttgcgatatcgatgccttctcgatc + aatgatgcgactttttgcaactggataaatagtccactttgaggatactctgtttgaaaa + tacttcttccccatgaatgatccatcgttcttaccaatgttggcaagtaagtctacacca + gcaaacattccagctttcgtgtccactggacccacgtatttcagttgtccgcgccccaaa + tttgggatttgtatgaaacatcctatctttctttgatatctatccatggtatttcaaacg + catacacagccagccacagctcaacgccttttaccttgtcctttgatgcctgtctcgtcc + aaacgtttttggtgtcttggccaattgcccttctgaaaaatctcactgtccgcaactcat + taaaagatacccaagcaagctacacgataaagaaaggagaaagttcattaaaagaacgta + catatagcgatacaaacgtatagcaaagatctgaaatggatacggataagttaatctcag + aggctgagtctcatttttctcaaggaaaccatgcagaagctgttgcgaagttgacatccg + cagctcagtcgaaccccaatgacgagcaaatgtcaactattgaatcattaattcaaaaaa + tcgcaggatacgtcatggacaaccgtagtggtggtagtgacgcctcgcaagatcgtgctg + ctggtggtggttcatcttttatgaacactttaatggcagactctaagggttcttcccaaa + cgcaactaggaaaactagctttgttagccacagtgatgacacactcatcaaataaaggtt + cttctaacagagggtttgacgtagggactgtcatgtcaatgctaagtggttctggcggcg + ggagccaaagtatgggtgcttccggcctggctgccttggcttctcaattctttaagtcag + gtaacaattcccaaggtcagggacaaggtcaaggtcaaggtcaaggtcaaggacaaggtc + aaggtcaaggttcttttactgctttggcgtctttggcttcatctttcatgaattccaaca + acaataatcagcaaggtcaaaatcaaagctccggtggttcctcctttggagcactagctt + ctatggcaagttcttttatgcattccaataataatcagaactccaacaatagtcaacagg + gttataaccaatcctatcaaaacggtaaccaaaatagtcaaggttacaataatcaacagt + accaaggtggcaacggtggttaccaacaacaacagggacaatctggtggtgctttttcct + cattggcctccatggctcaatcttacttaggtggtggacaaactcaatccaaccaacagc + aatacaatcaacaaggccaaaacaaccagcagcaataccagcaacaaggccaaaactatc + agcaccaacaacagggtcagcagcagcaacaaggccactccagttcattctcagctttgg + cttccatggcaagttcctacctgggcaataactccaattcaaattcgagttatgggggcc + agcaacaggctaatgagtatggtagaccacaacacaatggtcaacaacaatctaatgagt + acggaagaccgcaatacggcggaaaccagaactccaatggacagcacgaatcctttaatt + tttctggcaacttttctcaacagaacaataacggcaaccagaaccgctactgaacgatga + ttcagttcgccttctatccctttgtttacgtatttgtttatatatataactttatttttt + ttattaattgggctgcaagacaattttgttgtcagtgatgcctcaatccttcttttgctt + ccatatttaccatgtggaccctttcaaaacagagttgtatctctgcaggatgcccttttt + gacgtattgaatggcataattgcactgtcacttttcgcgctgtctcattttggtgcgatg + atgaaacaaacatgaaacgtcagtaatttgaaacaaataacgtaattctcgggattggtt + ttatttaaatgacaatgtaagagtggctttgtaaggtatgtgttgctcttaaaatatttg + gatacgacatcctttatctttttcctttaagagcaggatataagccatcaagtttctgaa + aatcaaaatggtagcaacaataatgcagacgacaacaactgtgctgacgacagtcgccgc + aatgtctactaccttagcatcaaattacatatcttcgcaagctagttcctcgacgagtgt + aacaacagtaacgacaatagcgacatcaatacgctctacaccgtctaatctactcttttc + taatgtggcggctcagccaaaatcatcttcagcaagcacaattgggctttcaatcggact + tcccatcggaatattctgtttcggattacttatccttttgtgttatttctaccttaaaag + gaattcggtgtccatttcaaatccacccatgtcagctacgattccaagggaagaggaata + ttgtcgccgcactaattggttctcacggttatttcggcagagtaagtgtgaggatcagaa + ttcatattctaatcgtgatattgagaagtataacgacacccagtggacctcgggtgataa + catgtcttcaaaaatacagtacaaaatttccaaacccataataccgcagcatatactgac + acctaagaaaacggtgaagaacccatatgcttggtctggtaaaaacatttcgttagaccc + caaagtgaacgaaatggaggaagagaaagttgtggatgcattcctgtatactaaaccacc + gaatattgtccatattgaatccagcatgccctcgtataatgatttaccttctcaaaaaac + ggtgtcctcaaagaaaactgcgttaaaaacgagtgagaaatggagttacgaatctccact + atctcgatggttcttgaggggttctacatactttaaggattatggcttatcaaagacctc + tttaaagaccccaactggggctccacaactgaagcaaatgaaaatgctctcccggataag + taagggttacttcaatgagtcagatataatgcctgacgaacgatcgcccatcttggagta + taataacacgcctctggatgcaaatgacagcgtgaataacttgggtaataccacgccaga + ttcacaaatcacatcttatcgcaacaataacatcgatctaatcacggcaagaccccattc + agtgatatacggtactactgcacaacaaactttggaaaccaacttcaatgatcatcatga + ctgcaataaaagcactgagaaacacgagttgataatacccaccccatcaaaaccactaaa + gaaaaggaaaaaaagaagacaaagtaaaatgtatcagcatttacaacatttgtcacgttc + taaaccattgccgcttactccaaactccaaatataatggagaggctagcgtccaattagg + gaagacatatacagttattcaggattacgagcctagattgacagacgaaataagaatctc + gctgggtgaaaaagttaaaattctggccactcataccgatggatggtgtctggtagagaa + gtgtaatacacgaaagggtactattcacgtcagtgttgacgataaaagatacctcaatga + agatagaggcattgtgcctggtgactgtctccaagaatacgactgatgaaaataatattg + acgttcgcatttaatctatacctataattctgtacttatatactgttccttaattgaaga + tttcaacatcgtttttgatgtaggtcttttcacctggaggtgcggctggggtaccgaaga + ctaattgagcttgtacggtccaagactcagggattttgcttggcaaagcagcttttatgt + aaccattgtagtgttgtaggtgaccacccaggcccattgcctccaaggcaacccacgagt + tgatttgagcggcaccagaggtatggtccgcgaaactagggaatgcagctgcgtacgctg + ggaagtcagcctttagcttttcagttaccttgtcgtcggtgaagaagattacagaaccaa + aggcctcatcccttgctgaagcaggcctcttttgaccggcaggctttctatagccttagt + cacttcgtcccaaacttttttgtgagtttcaccagtcaagataacagcgcgatttggctg + ggagttgaaagcggtgggtgtttctttaatgatggtttggacgacggattggatgtcgtt + gatagtaatttcaccaggtaactccggtttcaaagcgtaaatagtacgacgagcagttaa + agttttcaaataagttgcaaccagcagacatgatattggattgtttctttgtatatatgt + tgatataggatacttcagtctacgaaaaaagtacaaattatgtagtcagttccttcagca + tggtgtccttatatactgtagtttggacaaggtgcaaatgccaagaccctagcccgaaaa + gctcgaggcaccccaggatctttttctttacgtaattttcacgtaaaacgccacagtccg + atttttttcgaataatcattagtaaaagcggtatactggattattgtacgataacaaggt + agagctttattactaagctaagacgttcttacatcaatagtgctgttcgttattgatgtt + aggagaaggagagggtctgctgaatagtgtgagcagtgtttctgaactttttcttcgtct + aagtccttgtaatgtaaggtaagaatgcaagcatcttgtttgtaacgcgattgtacgttg + acgttagtaagtcacaaacccaagcttaacttcttcgtgaggaaggaaagtgttgtctcc + tactttttttaaattttcgaattgtatttatatttatttagtacttcttgagtttacata + tccttcgtaaaaatgcaacttctgtcgaaaaacacttccaaaaaaaaataataatgaatt + tatgaagcatactaacgagcgagcacatcgctgaggtatcattacttcatgagataaatt + aagatctcctcatatgcgaatttcctgttcagtgataaacgttgattacgttattgataa + aagtcttttcttctggcaaggcagttggaacaccaaagaccaattgagattgtacagtcc + acgcaataggaacatcttgaggcaaagcagatttgacgtagtcattatagtgttgcaaat + tagcccccaatcccaatagttcgagggcagtccaagactgaatttgcacagcaccggtcg + tatgagcggcgcatgttgggaaagcggtgccaaggctggaaaatctcttgcagttttcag + ttggtccttcatcagtgaagaaaatgactgaaccgtaagcctcatctctgcaagactctg + gtctcttcttggcttcagcagttggcattgcgctcgcaacagcatcccatatccttttgt + gtgtatcaccaacgataatgacagcgcgattcacttgtgagttaaaagctgttggcgtat + tcttgagaataacgtgtacagttctctttacatcatccaaaccgacaccttgtggtaatt + cgggcttcaaattgtagatggtacgacggtttgtaatagcgtttaagtagtttccagttg + gggacatttctttggcttggaggtctggtgttcttgattttgatggtgtatatagcttta + aaaaaccaaaaatgatcaacctttatatctctcttcgatcacaacatgggtggagacaaa + cacaaagtagtgatgacccactcaggtgttaaagtgtgtcatatctgcaaatgacactgt + atttttttttcctttgccatttattgtcgcagtaaggaaaagcgcagagcaaagctgaca + aattgaaaaattattcggaagagcattaacaaatattgattaataatcaagtaacactcg + cagacattgttatttcagaagtaaaaatatcgcagaacaagttggttaaactgaatgctt + cagtcattctaatgctaattacaccttaacaaaattaaaaattcttaacgtagattttta + aaaaaggcaagaagatcgttcctgatgcacgacgaaaacggttgcacccctactaaggca + aaattgcatatgcggatgaaatcaaacctcttctgttgcattaagaacaaaaggaaatca + tttttctctcacttcatattatttcacttatattttcctctccatttccatcacgcaatc + gttagtgctttttattttttttcttcgctcataaaggactagaattaaaatgaaaatcgt + ctatcattacacgtatgctattactacaaaacagaagtacaaaaatgaataaatataaaa + gaagtaaatgctttttttttaacaccagaaggcaacgacccttttccaataaggtccgtt + cctcaaacgttccctatattcttcgtcttcttgcttgattaattcttcatcaaagatttg + tctatgagaatctaggtcgatcttgtcggccctgatgaacagtttccaatccttgtgcca + gaccttgtagccgacatatagtgcaatcaagattggcatagccaagtagttttcgaaaaa + ggcttgtgcatccagcttaccttcaccaatgggggcgatagcgacccaaaattgggcaat + aagaattaaaatcatcataatgcaagcgtaggcagatccccaaacaccagtttgagattt + gaaacccaattctccaagagatctcccttggactttcatggctcttctaaatctaagatg + ggataaacaaatggcagtccatgtgaaaagctgagacaaaccagaaatggccaataacca + agtgaaaacttgttcttccttgggagatgcagcacagaaggcaataacggcaaacaatgc + agaaacacccatggcaatcaatggtctaccggctctgtcgatgtaggagaaaaccttagg + agcgtaaccttgctcggatagagttaaaaataaacgagcactggagtagaaggaggagtt + agccatggacagcacggaaagtagaataacggcgttaatgaagtgtgggacgacacggac + accgtgggatgcaacagcaatgacatatggcgaggctttagtaccaccaccggtagaacc + caataattgatcggaattgtatggcaccaagaaacccagtagaataatggtagccaagaa + taggaataagattctgtagatcatttgtttggccgcacctggaatggcctttcttggatt + agattgttctgcagtggtgatggcaataaactctgaaccaccaaaggcaaaagcagcagt + cactaatgttgcaacaacacctttaaatctgtcaatggcatgtttaccattgaaagcgcc + cggatcgtgccagtatttaccaccaataaaaccatcattaccagcgccaccaacatcgat + gataataccaagaatgaagaacccagtcatcatcaaaattttgcaacagttgaagaagaa + ctcagcttctgcataaccacgagcaccgaaaatattaatagtaatcaccaaaacatagaa + aataatgacgaacacatccgggttcacagatgtcgtccaatatttgatagtcatggatgc + ggtcaccaattccagaggacacacacacagccattgcaaacaataaacccaagcgactgc + aaacccaaaaaccatcatccacaaggaaactgggtatgcattgtagccaccagtcaagtt + actatacactagcgccatttcaccacatgcttgaataatacagtacaagatagaacccat + aatagcgtaaccaataagtagtccagctggacccgcatgaaccaacgcggtaccgttacc + gaccagtaacccagtaccgatacccgtacccaacgcaatcatcagaacatgtctaggctg + aatggttttcttcagcgagtccgacttatgacctgtattagcgcctatgttgtccgaact + ttcatttttttctagttcttgagcctgtctagaagaaggtgagatagccgacgtaagatc + gttcatttgtgttgcttctacttcatcctgtggaccctgatccgctcttttaaaggagtc + gaaaaacctacgcacggcagaagtgttatgctgttcgtaacctaaatgaggttgtgagga + tggacggtcattggagcctgtttcgaaatattcaatttcattatcctgccccgtggcatg + tatttctgtggagctatttttcaagtctttcagttcgtatagagacttcgacgacgacat + tgtgcgaagctatctttgtctatattagcgtgcttcttctataaagattacataataaac + ttgttctacattgttgtgggcagaccatcaaagacaaagcacttatatatgtatgtaaaa + caaatgagtattacgaggaaataacaaaagaaactaatcacgaaggatatgagtcttgtt + agcggacgaggggaagatagcgcaagacacacgtatagccgacctatcctatgcatctta + gccgtcttatcaggattgatttgcggttcagtgaaagagataaccactgccaagctggaa + gaaatggctagaaggcactaaaaaaagagcctcgcagcgcaacttaagaactgtggacta + ctaaggagcagcaatccgctggacgtgggggagttgaaagttggccggatactgcaacct + gcttatctatgactcgatgagaccgttatttagtgcgatggtgccactgggagtgccatc + aacggcaaagttgcagctttttgcagcagatcactggcttagacggcaccctcaagcggc + actgaagaacggcgcgcttttttttttcttctattatgccggtcaaggaaaaaaagaaac + caaaaaaaaatgccgtgggaggcacggatgcaagatttctccaaagtccctttttagccg + ccgaggaaatgaggaaataaggggttagccgccgagtgaagccgccgaggtataaaaggg + agaatgcacaccttcgtaagaagattctatttgaaagagtgctaggtaaagtcatcaaaa + ggcttattgatataataattgctttgcaaaatggaaaacaaatgactttgaaaatgggag + aaaacaaaaattatataggtaatgttgagttcttgattttttttttgtttctgtccttgc + cacagctcttttcatggaacattgggctctaggataatttttttatgttttctgttatgt + ttgccatggtaggttgaggtaaaggcgctctttcagcaatgacgatttatgctaatatgt + tttccttaaaaggaaaacatctcgttctttttttcgcgtgctgtgcaagaaaacgcgtaa + cgcagtcttagtaaaagggctctcttgaaaactacatgaaaactaaaaaagaagatgtaa + atttggatacaacagcgtagaatagcataatagagtgtgcaataaacgtgtataggcttg + ataatagtgctgacgtaaattaccagaactactgcagtatgactgtggcgaataccgaga + cccattctgctgctaaaccatcgagtactataggcccatggaagcttggtgaaacactgg + gctttggtagcaccggcaaggtccaacttgcgcaacatgagcgcacgggccatagaacag + ccgtcaaagtaatctcaaagtccattttcaacaacaacggaaaccacagtaacgacgatt + cagtgctgccatacaacatcgagcgtgagattgttattatgaaacttttgagccacccaa + acgttctgagtctttacgacgtttgggaaaccaacaataacctgtaccttatcctggaat + acgccgaaaagggagaactgttcaacttgcttgtggaccacggtcccttgcccgagcgtg + aggccatcaattgcttcagacagataattataggcatttcatactgccatgcgctaggaa + tagtacatcgggatttaaagccagaaaatcttttgctcgatagtttctataatatcaaaa + ttgcggattttggtatggcagctttacagacagacgccgacctgctagaaacttcttgtg + ggtctcctcattatgccgctcctgagatcgtatcaggtctgccctatgaaggatttgcca + gcgacgtttggtcttgcggtgtaatcctctttgcccttttaacgggtagactacccttcg + atgaagaaaacggtaatgttagggatttgttattgaaagtccaaaagggccagtttgaaa + tgcccaatgacaccgaaatttccagagatgcacaagatttaatcggcaagattctcgttg + tagatcccaggcaaagaataaaaatcagagacatccttagtcatccactgctaaaaaaat + atcaaaccattaaggattccaaaagtatcaaagatttacctcgtgaaaatacctatctat + atccactggctgactcgaacaaccatactagcgcctctatcgatgattcaatcctacaaa + acttggtggtactttggcatggtagacacgccgatgacatcgtatctaaactgaaagaga + atggaaccaacaaagaaaaaattctatatgcgctactataccgtttcaaactggactctg + taaggggatcgaataagaagaaccgcaataagataaagaaaaccaaaaaaaataaaagat + ctagcacactttcgtcatcgtcatcattattattgaacaaccgaagcatacaatctacgc + cacgcagacgtacatccaagaggcattccagggaattctcttctagcaggaagagatcgt + cctttttactgtcctcgaatcctaccgatagttctccaatacctttgagaagtagtaaaa + gaattacacatattaacgtagcgtctgcaaatacccaagcaacgccaagtggtgtcccga + atcctcacaagaggaactcaaaaaaacggtcaagcaaaagattatcttatatgcctaata + caaaaagaagctcactaacatcaaaatcattatcaaactttactaacctaatagatgatg + atgattgggagtacattgaaaaggatgcaaagagaacaagttccaacttcgctacactga + ttgatgaaatatttgagcctgagaaatttgaattggcgaaaagagaaaaggctgaacttc + aaagaaaagttcaggaagcaaaaaggcaatcagtgaatgcacagaagattaatgaggacg + agtttggatccgaagtttctgatggaatgaaagagctgaaaaaaataaatgacaaagtgt + cgtccccgttgataaattacgaattttcgcaacaagaactattgcaagatatagacacct + tactaacgaatcgttatcaactttcgtcatatactaggcctatttcgagactggatcccg + gattaacgcctgttactgagacacttcctaacaacctaaaagaaaaaacagctctgctgc + aggatactgaaaagaaaataatagaaacgatacgcagatccaaatttttaggatcgctac + taaatgttagagggggactatcgccagggaaaagtgaactggcacctatcgaagagtctc + ccatagtttcaaccacaccactaatatataatgatcgaatggaacctcgtaggatatccg + atgtggaagtcccacatttcacgaggaaatcaaaacactttactactgctaataatcggc + gctcagtcttatctttgtatgcgaaggattcaatcaaagacttaaacgaatttttaataa + aggaagatcctgatttgcctccacaaggaagcactgataacgaaagtaggagcgaagatc + ccgaaatagccgagagtatcactgattcaaggaatatacaatatgatgaggatgatagta + aggatggtgataatgtgaataatgataatatattgagcgactttccctcaaggcgttggc + atatcacaggaatacgacatgaaggataaaaatccaaaccaatctccaatatcaaaaagc + gcagagcccacactggtagtgaaacttccgtctttgagttctttccaaggaaaaaacgcc + agtgggttgggcctataccaaagagagccttctaaggtaaccttaccgagccttacaagt + aataacagcagcgtcggagaaaacactagaggatggggcggaaaaagggactgagagtga + gaaaatcgctgcttccctgtcagacgatgacttgaaggaagataatgacaagaaagataa + cgacacagtgaatgccctactacagtaaaaaagccacctaacagcgtactcttgaaaaaa + ttctcaaaaggtaaaatcttagaacttgaaatacatgccaaaatacctgaaaagagacta + tacgagggcctacataaactactagaaggctggaagcaatacgggttgaaaaacctggtg + tttaacatcactaatatgatcataacaggaaaattggtgaatgacagtattttattttta + cgatctacgctttttgaaataatggttttaccaaatggagacggtagaagtttaattaaa + ttcaataagaaaacaggatctactaaaacgctgaccaagcttgctacagaaatccaaatt + attttacaaaaagaaggtgttttggacaaataaaaaaaagggagggataatctatcccca + aatggacaatacgatgtaacaccgttatataattaataaatgtatatatgtaactatttt + ttgaaaaaaataaaatggtaatttattaaatatttggaccggtcaacaatctcagatata + gttgaaaaaatctttaattgtaatagcagaaaaaaaaaactccgatacggggagtcgaac + cccggtctccacggtgaaagcgtgatgtgatagccgttacactatatcggataacaaatt + attaaaattatttaataaaaagatgaactaatggtgtataaaaacatcataaaaactcaa + ctcaactgttccaataaaatctgcaatttaaattaattattctgaaaaaacacgtaaatt + gttggaatggaaccctaaaatatttcctctttagtactattcatgttacttgagaaatgg + gtgaattttgagatagttgttgggattccattgttgataaaggctaataatattaggtat + acagaatatgctagaggttctcctcgagcatataggaatcctcaaaatggaattagcatt + tctacataataacctcaattcccttttgtggattcacatattctcgatcggaactcctag + catagtgcagacaacactcacatagctctcccatttatcaagttggaattcactccaaga + aattttcttgcttctctctttcccacagaaatgaaaaggaacagtttggcggctattgtt + ttagcccttactttttggaccctttgaaaaaatgccaagaatgcgtgacggtttccctcc + atgctcgagaaccacaaaaaccctatgtggtattcgattatgcgactcacggcaaatatt + tgacttgtcaaagttattcgggatttccttgaatccctgagaagtgaaatggaaagaatt + taagggcataaatacctttcaagcacccagaagaacagtaaaataaagcaaggtacgtga + aattaatatttttaaatggttctaaccgatgccgaagaactgcgcgatccggttataacg + tctgacatgtccttttttgatttggaatccaaccactcagtgactctgtcattacttgcg + aaattattccacaaattccatcgaaagtgaatcgcaaaccaccttcagactggcaccgac + aaagcaaagattatacagacagagtactttatacgtaccgttaagtctcaagcaaagggt + tttcttatttactgaacgggtaaagagtatctgggccggcttgccaagatgcaaaccgaa + taagtatttcaaagttgcatttgccttagccgtcctgacaccattggctatttggatatt + ttatattgactttcgtgtacattgatcacatcgactgttctattggcaaatgaaccacgg + gcattgactatttttcaggttactactatatattattgttggaataaaaatcaactatca + tctactaactagtatttacgttactagtatattatcatatacggtgttagaagatgacgc + aaatgatgagaaatagtcatctaaattagtggaagctgaaacacaaggattgataatgta + ataggatcaatgaatattaacatataaaatgatgataataatatttatagaattgtgtag + aattgcagattcccttttatggtgttggaataaaaatcaactatcatctactaactagta + tttacgttacctagtatattatcatatacggtgttagaagatgacgcaaatgatgagaaa + tagtcatctaaattagtggaagctgaaacgcaaggattgataatgtaataggatcaatga + atattaacatataaaatgatgataataatatttatagaattgtgtagaattgcagattcc + cttttatggattcctaaatcctcgaggagaacttctagtatatctacatacctaatatta + ttgccttataaaaaatggaatcccaacaattacatcaaaatccacgttcttttcatatgg + attcctaaatcctcgaggagaatttataatatagtctgtatacataatattatagcttct + aacgacaatggaattctaacaattatcaaattgccgccagttcctcaaaaagatccatgt + ataatcttcattattacagccctcttgacctctaatcatgaatgttctcaaataaatctg + ttggaataaaaatcaactatcatctactaactagtatttacgttactagtatattatcat + atacggtgttagaagatgacgcaaatgatgagaaatagtcatctaaattagtggaagctg + aaacgcaaggattgataatgtaataggatcaatgaatattaacatataaaatgatgataa + taatatttatagaattgtgtagaattgcagattcccttttatggattcctaaatcctcga + ggagaacttctagtatatctacatacctaatattattgccttataaaaaatggaatccca + acaattacatcaaaatccacgttctcttcatggtagcgcctatgcttcggttacttctaa + ggaagtcccatcaaatcaagatccgttagccgtttcagcttccaatttaccggaatttga + tagagattccactaaggttaattctcaacaagagacaacacctgggacatcagctgttcc + agagaaccatcatcatgtctctcctcaacctgcttcagtaccacctccacagaatggaca + gtaccaacagcacggcatgatgaccccaaacaaagctatggcctctaactgggcacatta + ccaacaaccgtctatgatgacgtgttcacattatcaaacgtcacctgcgtattatcaacc + ggacccacactatccgttgccacagtatatcccaccactgagtacttcctcacctgatcc + aatcgattcacagaatcaacactctgaagtacctcaagctgagacaaaggtgagaaataa + cgtcttaccaccacacactttaacatcagatgaaaacttttctacatgggttaaatttta + catcagatttttgaagaactctaatctcggtgacatcattccaaatgaccagggtgaaat + caaaagacaaatgacttatgaagaacatgcgtatatatacaataccttccaagcatttgc + cccatttcatttattgccaacatgggtaaaacaaattttagaaattaattatgctgacat + ccttacagtcctttgtaaaagtgtgtccaaaatgcaaactaacaatcaagaattaaagga + ttggatagctcttgccaaccttgagtacgacggaagtacatctgctgatacatttgaaat + tacagtcagtacgatcattcagaggctaaaagaaaacaatatcaatgttagcgacagatt + ggcctgtcaactaatacttaaaggtctatccggtgacttcaaatacctacgtaatcaata + tcgtaccaaaacgaacatgaaactttcccaattattcgctgaaattcagttaatatatga + cgaaaataaaatcatgaatctaaataaaccgtcccaatacaaacaacacagcgaatacaa + aaatgtttctcgcacatctccaaacacgactaacacaaaggttacaactcgtaattatca + gagaacaaatagttcaaaaccaagagcagcaaaagctcacaatattgctacatctagtaa + attctcaagggtgaacaatgatcacattaatgaatcaaccgtttcatcacaatacttaag + cgatgacaacgaacttagtcttaggccagcaacagaaagaatctaagccaacacacacaa + tagactcgaatgacgaactacctgatcaccttcttattgattcaggacgttcgcaaacgc + ttgtcagatcagcccattatttacaccatgcaacacccaattctgaaataaacatagtcg + atgctcaaaaacaagacattcctataaatgccattggtaatcttcacttcaactttcaga + acggcaccaaaacatcaataaaagcactacacacaccaaacatagcctatgatctattaa + gtttgagtgagctggctaatcaaaatattacagcctgctttaccagaaacactttagaaa + gatcggatggtacagtcctagctcccatagtcaaacatggagacttttactggttatcta + aaaaatacctaattccttcgcacatttcaaagctaacaataaacaacgtcaacaaaagca + aaagcgtaaataaatatccatatccgttaatacatcgaatgcttggacatgctaacttcc + gaagtattcagaagtctcttaagaagaatgcagttacatatttgaaggaatcggatattg + aatggtctaacgcatgcacatatcaatgtcctgactgtctaatcggcaaaagcacgaaac + atagacatgtcaaaggatcacgactaaagtaccaagaatcatatgagccttttcagtact + tgcataccgatatatttggtcctgtacatcacttaccgaaaagtgcaccttcttacttta + tatcgtttacagatgagaaaaccagattccaatgggtgtacccattacacgaccgtcgtg + aagaatctatcctcaatgtttttacatcgatattagcatttattaagaaccaattcaatg + ctcgcgttctagttatccagatggatcgtggctccgagtacactaacaaaactcttcata + agttctttacgaacagaggtattactgcatgctatacaaccacggcagattctagagcac + acggtgtcgctgaacgattaaatcgtactttattaaacgattgtcgcacactgcttcatt + gcagtggtctaccaaatcatctatggttctcagcagtcgaattttctactataatcagaa + attcattagtctcaccaaaaaacgataaatccgccgcaagacaacatgcaggtttagctg + gactggacattactactatactacctttcggtcaaccggttatagttaacaaccataatc + ccgactcgaaaatacatcctcgtggcattccaggttacgccttacatccgtcacgaaact + cttatggctatattatctatcttccatcattaaaaaagacagtagatactaccaattacg + ttatattacaagacaagcaatccaaattggaccaattcaattatgatacactcacctttg + atgatgatctcaatcgtttaacagcccataaccaatcttttattgaacaaaatgaaacgg + agcagtcatatgatcaaaatacagaatctgatcatgactatcaatcggagattgaaataa + actctgatcctctagtgaacgacttctcgtcccaatcaataaaccctttacaattagaca + aggaaccagtccaaaaagtacgtgcaccaaaagaagttgatgccgacatatctgaataca + atattcttccatctcctgtacgatctcgtacaccccatatcattaataaagagagtaccg + aaatgggtggtaccgttgaatcagatactacttcacctagacactcgtctaccttcactg + cacgaaaccaaaagcgacctggtagtcccaatgatatgattgatttgacctcacaggata + gagttaattatggacttgaaaacatcaaaactacacgtttgggtggtacggaggaaccat + atattcaacgaaatagtgatacaaatatcaaatacaggactacaaatagtacgccctcaa + tagatgaccgttcgtccaacagtgaatccactactcccatcatctccatagaaacaaagg + ctgtatgtgataatacaccctccattgatacggatccgccagaatatcgatcttctgacc + atgcgactcctaatataatgcctgacaaatcctcaaaaaatgttacggctgattctattc + ttgacgacctcccacttcctgacttaacccatcaatctcctacggacacttctgatgttt + caaaagatattccacacatacactctcgtcagactaattccagtttgggtggtatggatg + attctaatgttctgactactaccaaaagtaagaaaagatcattagaagataatgaaactg + aaattgaggtatcccgagacacatggaataataagaatatgagaagtctggaaccaccaa + gatcgaagaaacgcataaatttaattgcagcaataaaaggagtgaaatcgatcaaaccag + ttcgaacgaccttaagatatgatgaagcaattacttataataaagacaacaaagaaaaag + acagatatgttgaagcttatcataaagaaattagccaactattgaaaatgaacacttggg + atacaaacaaatattatgatagaaatgacatagatcctaaaaaagtaataaactcaatgt + ttatatttaacaagaaacgtgatggtacacacaaagctagatttgttgcaagaggcgaca + ttcaacaccccgatacatatgattctgatatgcaatccaataccgtacatcactatgcac + tgatgacgtcactgtcaatcgcattagacaacgactattatatcacacagctggacatat + cctctgcttacttatatgctgatatcaaagaagaattatacataagacctccaccacatt + taggtttgaatgataaattactacgtttgagaaaatcactctatggtttgaaacaaagtg + gtgcaaactggtatgaaaccattaaatcatatttaataaattgttgcgacatgcaagaag + ttcgcggatggtcatgcgtatttaagaatagtcaagtaacaatttgcttattcgttgatg + atatgatattattcagcaaagacttaaatgcaaataagaaaatcataacaacactcaaga + aacaatacgatacaaagataataaatctgggtgaaagtgataacgaaattcagtacgaca + tacttggattagagatcaaatatcaaagaagcaagtacatgaaattaggtatggaaaaat + ccttgacagaaaaattacccaaactaaacgtacctttgaacccaaaaggaaagaaactta + gagctccaggtcaaccaggtcattatatagaccaggatgaactagaaatagatgaagatg + aatacaaagagaaagtacatgaaatgcaaaagttgattggtctagcttcatatgttggat + ataaatttagatttgacttactatactacatcaacacattgctcaaccatatactattcc + cctctaggcaagttttagacatgacatatgagttaatacaattcatgtgggacactagag + ataaacaattaatatggcacaaaaacaaacctaccaagccagataataaactagtcgcaa + taagcgatgcttcatatggtaaccaaccatattacaagtcacaaattggtaacattttcc + tactcaacggaaaagtgattggaggaaagtcgacaaaggcttcgttaacatgcacttcaa + ctacagaagcagaaatacacgcggtcagtgaagctattccgctattgaataacctcagtc + accttgtgcaagaacttaacaagaaaccaattattaaaggcttacttactgatagtagat + caacgatcagtataattaagtctacaaatgaagagaaatttagaaacagattttttggca + caaaggcaatgagacttagagatgaagtatcaggtaataatttatacgtatactacatcg + agaccaagaagaacattgctgatgtgatgacaaaacctcttccgataaaaacatttaaac + tattaactaacaaatggattcattagatctattacattatgggtggtatgttggaataaa + aatcaactatcatctactaactagtatttacgttactagtatattatcatatacggtgtt + agaagatgacgcaaatgatgagaaatagtcatctaaattagtggaagctgaaacgcaagg + attgataatgtaataggatcaatgaatattaacatataaaatgatgataataatatttat + agaattgtgtagaattgcagattcccttttatggattcctaaatcctcgaggagaacttc + tagtatatctacatacctaatattattgccttattaaaaatggaatcccaacaattacat + caaaatccacattctcttcaaaatcaattgtcctgtacttccttgttcatgtgtgttcaa + aaacgttatatttataggataattatactctatttctcaacaagtaattggttgtttggc + cgagcggtctaaggcgcctgattcaagaaatatcttgaccgcagttaactgtgggaatac + tcaggtatcgtaagatgcaagagttcgaatctcttagcaaccattatttttttcctcaac + ataacgagaacacacaggggcgctatcgcacagaatcaaattcgatgactggaaattttt + tgttaatttcagaggtcgcctgacgcatatacctttttcaactgaaaaattgggagaaaa + aggaaaggtgagagccgcggaaccggcttttcatatagaatagagaagcgttcatgacta + aatgcttgcatcacaatacttgaagttgacaatattatttaaggacctattgttttttcc + aataggtggttagcaatcgtcttactttctaacttttcttaccttttacatttcagcaat + atatatatatatatttcaaggatataccattctaatgtctgcccctaagaagatcgtcgt + tttgccaggtgaccacgttggtcaagaaatcacagccgaagccattaaggttcttaaagc + tatttctgatgttcgttccaatgtcaagttcgatttcgaaaatcatttaattggtggtgc + tgctatcgatgctacaggtgtcccacttccagatgaggcgctggaagcctccaagaaggt + tgatgccgttttgttaggtgctgtgggtggtcctaaatggggtaccggtagtgttagacc + tgaacaaggtttactaaaaatccgtaaagaacttcaattgtacgccaacttaagaccatg + taactttgcatccgactctcttttagacttatctccaatcaagccacaatttgctaaagg + tactgacttcgttgttgtcagagaattagtgggaggtatttactttggtaagagaaagga + agacgatggtgatggtgtcgcttgggatagtgaacaatacaccgttccagaagtgcaaag + aatcacaagaatggccgctttcatggccctacaacatgagccaccattgcctatttggtc + cttggataaagctaatgttttggcctcttcaagattatggagaaaaactgtggaggaaac + catcaagaacgaattccctacattgaaggttcaacatcaattgattgattctgccgccat + gatcctagttaagaacccaacccacctaaatggtattataatcaccagcaacatgtttgg + tgatatcatctccgatgaagcctccgttatcccaggttccttgggtttgttgccatctgc + gtccttggcctctttgccagacaagaacaccgcatttggtttgtacgaaccatgccacgg + ttctgctccagatttgccaaagaataaggtcaaccctatcgccactatcttgtctgctgc + aatgatgttgaaattgtcattgaacttgcctgaagaaggtaaggccattgaagatgcagt + taaaaaggttttggatgcaggtatcagaactggtgatttaggtggttccaacagtaccac + ggaagtcggtgatgctgtcgccgaagaagttaagaaaatccttgcttaaaaagattctct + ttttttatgatatttgtacataaactttataaatgaaattcataatagaaacgacacgaa + attacaaaatggaatatgttcatagggtagacgaaactatatacgcaatctacatacatt + tatcaagaaggagaaaaaggaggatgtaaaggaatacaggtaagcaaattgatactaatg + gctcaacgtgataaggaaaaagaattgcactttaacattaatattgacaaggaggagggc + accacacaaaaagttaggtgtaacagaaaatcatgaaactatgattcctaatttatatat + tggaggattttctctaaaaaaaaaaaaatacaacaaataaaaaacactcaatgacctgac + catttgatggagtttaagtcaataccttcttgaaccatttcccataatggtgaaagttcc + ctcaagaattttactctgtcagaaacggccttaacgacgtagtcgacctcctcttcagta + ctaaatctaccaataccaaatctgatggaagaatgggctaatgcatcatccttacccagc + gcatgtaaaacataagaaggttctagggaagcagatgtacaggctgaacccgaggataat + gcgatatcccttagtgccatcaataaagattctccttccacgtaggcgaaagaaacgtta + acacaccctggataacgatgatctggagatccgttcaacgtggtatgttcagcggataat + agacctttgactaatttatcggatagtcttttgatgtgagcttggtcgttgtcaaattct + ttcttcatcaatctcgcagcttcaccaaatcccgctaccaatgggggggccaaagtacca + gatctcaatcctctctcttggccaccaccggatagtaaaggttctaatctaactcttggt + ctccttcttacatagatggcacctattccctttggaccgtaaatcttgtgagaagaaatt + gatagtaaatcaatgttcatttcattgacatcaatgtgaatcttaccataggcttgtgcg + gcgtcagtatgaaagtagatcttattctttctacaaattgcaccaatttctttaataggt + tgaatgacaccgatttcattattgacagccatcacagagacgagacaggtatctggtcta + atggcatcttccaattccttcaaatcgataagaccttgatcgtccacatttaggaaagtg + acttcaaatccctccttcatcatggcccgtgcggcttccaagacacacttgtgttccgtt + ctagtggtgatgatgtgtttcttagtcttcttataaaatcttgggacacccttaagaacc + atattattagattcggtcgctcccgaagtgaatattatttccttggggtcggcattgatc + atctttgctacgtaagctctagcattttccacagcagtatttgtttcccaaccgtaagag + tgagtgttggaatgaggattaccataaagtcccgtataaaacttcaacatcgtatccaaa + accctagggtctgttggtgtagtggcttgcatgtcaagatatatgggacgagtaccaaaa + cctgtgttttcttgataagcatggctcattgcagtgctaccagaagctactacagcatct + ggggtggtaccggatgcactcgcacgggcactagcctgtgcctttgcagcagcctgaata + tcggtatgcgtttccagagagaagttgtcgtctaacttcacgcctgctgcaggaggggaa + tagaatctcctgcttaccaaacaagccctatatgtggccgctggaacgttgtaaacttga + gataatcttgttatcgatcttgtagcagttgatttcaacatcttccagttgtagcagttc + cttcgtgtatgcgatatattttaatgaaaatgatgtatctgaaatagaaaatataacaca + taatgaactctgtcggtctttactctgagtgagttacttagatagtcatcatatatccct + gggcgatggccttactatttgtcagcgaccgtatctaaaattggtaggtcactatcaact + attgcgggtaatagggtgccataatagtattgtaatatatataaatatataaagcatcat + atgatagatatttgttgctattcacgtcatccactattactttagtggttccgctatata + ttcgcattgcccatcgaaggcagttttgtgtgctacctgctcgtgaccacggtctttttg + cccagtcttttacggcgggcatacttcatgatgaaactgtctattttcatacctcttgaa + tttagttcttcaattagaggcttgatatcctccaagtcccactgtgactgtagcctaaac + aggactttaaaccgttctttggggtccattggtagtgtgcttttcgctatatactggaca + gtcttatcggtaggcttgaaatgatagcctcgcagcatgtcaatgtcaatgtcacatggg + aagaaaggtgggaaaagggacttccacttgatgaggaactcatcaattggcatgcttatt + ccagaaacatatttccttagcgcttgaatcccgtaccactgagctataaacggtattctc + aagcgccacgtattgttttcagcctcttgctcttgaacagcaaatttattcagcactgtt + tcaatgatttctcttgtgtatggattgaactcgtcctcaatgtccttccccacagcgtga + tgtgtatcctcaacattcagatgctgtagatcgagtgattctgccattgcgctcatcagt + agtacatgcagtgctttgaaaaggaagtcttgtgaaagaatacacaacacaccgtctttc + acagatccaccaatcttatgccattttgatataccttctagcgcagaacatggtgagttc + tcaagtagttcttccagggtcccgataacctttgtagatgacctcttcatgattttgtcg + gagaaatccagttctccgttgtatattggtactgaattcaagttcaattcgccatgtgtc + tctctggtctcaaattctgattcagtcttggcgaatcccacgacgtccatgtacggcttg + gacagtccaaacaagagcgtttcgtcgaaagtaataggttgttcaggaacaaattctctc + attagtagaactgtgtttgaatgtttgcgctgcttgcagcacccaagtcttgtcgtgcga + acacagtacaacttcagacttgtctttgtccaatgacttaaaccttaactggtgattttg + aaccggatcctgtattatatccagtaactctggtgtcaactggatcagcttataagatgg + atcatactcgggtgcggaatgtaggttgatggacatggtacaggattattagtgagcaaa + tctctggtggatttttaccgttcatctcatagtgcttttttagtttatttttcacttttt + tctggttatactatacgctgcctactgaagcaataagcaagaaacacaacagtataaagg + gacaaagataataaaccgcctactactaaataatatcgctgtggtatgctactcctagag + atttcgatcaccatcgtggtgctctttgtcatacgcatagaattgacaaaaaagaaccct + aagctataaattaggtcatgttgagcgattgccagttgccaagacctagggttttgttcg + aacaattctaaaaaggtaaataaaaacaatggtaaacaaaggctttgatcttcaatgagg + aagatttctctcactgcggatagtaaaaacaacactagtttgtctgtactcgcattctac + tgctgctacctgctactcattacgtgttcgggctgttatctggttgctaaaagagtatat + ttacacctcaccaatggagaaagacctgtcgtctctttactctgaaaagaaagacaaaga + gaacgatgaaaccttatttaacatcaaactatccaaatctgttgtcgagaccacaccgct + aaatggtcattcattgtttgatgatgataaatcactttcagactggacggataatgtgtt + cactcaatcagtattctatcacgggtcagatgacttgatatgggggaagttctttgtctg + cgtgtacaagtcccccaacagcaataagttgaacgctataatattcgacaaattaggaac + atcatgcttcgaatccgtcgatatatcttccaactcgcaatactatccggccattgagaa + tttgagtccaagtgatcaggaaagcaatgttaagaaatgcattgctgtcattctgttaca + gcgctatccattactttcaccatcagacttatcacaaatattgtccaataaatcggaaaa + ttgcgactatgaccccccttatgctggagatttggctagtagttgccagttgataacagc + agttcctccagaagatctggggaagcgcttctttacatcaggacttctgcaaaatagatt + tgtcagctctaccctgttagatgttatttatgaaaacaatgaatccaccatcgaactaaa + taataggttggtattccatctgggtgaacaacttgaacaactttttaacccagtcacaga + atactcaccggaacagacagaatatggttataaggcgccagaggacgaattacccacaga + atcggatgatgatcttgtcaaggccatttgcaacgagttattacaactacaaacaaattt + tactttcaatttggtagaatttttgccaaaattcctgatcgccttgagagtcagagtact + caatgaagaaattaatgggttatccacaaccaaattaaatcgactcttcccacctacaat + agatgaagtcacaagaatcaattgtatttttctagactcgctaaagacagcaatccctta + cggttccctcgaagtactgaaggcatgcagcattactattccttatttctacaaagcata + tacaagacacgaggcggccacaaagaacttcagcaaagatattaaattgtttattaggca + tttcagcaatgtaattccagaaagagaggtctacacggaaatgaaaatcgagagtataat + taagggacctcaggaaaaactactgaagctaaacttaattatagagagattgtggaagtc + gaaaaaatggagaccgaaaaatcaagaaatggcaaaaaaatgctacaacaatatcattga + tgtcattgattcgtttggaaaattagattccccacttcattcttatagtaccagagtatt + tactccatcgggaaaaatccttacagaattagccaaatgctggcccgtagaactgcaata + caaatggctgaagagaagggtagtcggtgtgtatgatgtagtggatttgaatgatgaaaa + taagagaaatttattagtcatattcagtgattatgtggttttcatcaatatactggaggc + agaaagttactacacttcagatggatcaaacaggcccttaatctcagatattttaatgaa + ctcattgatcaacgaagttccgttgccctccaagatccctaagttgaaagtggagcgtca + ttgctatatagatgaggttctagtttctatattagacaaaagcactctacgttttgatcg + attgaagggaaaagattctttctcaatggtatgtaaattatcctctgcatttatctcttc + ttcgtcagttgctgacttgattacgaaggctagaattttggaaaaagacactgcatttca + tttatttaaagctagtagaagccattttacattatattctactgctcacgagctttgcgc + ttatgattccgaaaaaataaaatcaaaatttgccttattcctgaacataccaccatccaa + ggagatattggaggtcaacaaccttcatttggctttttttgcaagattttgcagtaacga + tggtagagataacatcgtaatcttagacgtcttaaccaaacatgacgataaacatataga + agttacatccgataacattgttttcaccataattaatcaattggccattgaaataccgat + atgcttttcttccttaaactcatcgatggccaaagatttactctgtgtaaatgagaattt + gataaaaaacttagaacatcaattggaagaggtcaagcacccttcaacagacgaacatag + ggctgttaatagcaaactttccggtgcatccgatttcgatgctactcacgagaagaaaag + atcatacggtaccataacaacatttagaagctatacaagcgacttgaaggacagtccatc + aggcgataatagtaatgtcaccaaggaaactaaggaaattttaccagtgaaacctacgaa + aaagtcttcaaaaaaaccaagagaaattcaaaagaagaccaagacaaacgcctctaaagc + agagcacatagaaaagaagaagcctaacaaaggcaaagggttttttggcgtgttaaaaaa + tgtttttggaagtaaaagcaagagcaagccttcaccagttcaaagagtgcctaaaaaaat + atcgcagaggcatcctaagtctccagtgaagaagccaatgacctcagaaaagaaatcctc + ccctaaaagggcagtcgtttcatctcccaaaattaaaaagaaaagtacttctttttccac + aaaagaatcacaaactgctaaatcttctcttcgagcagttgaattcaaatctgatgactt + gatcggaaaaccacctgatgttggaaatggcgcacatcctcaagaaaataccagaatatc + ttcagtagtaagggatacaaaatatgtctcctacaatccctctcagcctgtgacagaaaa + taccagtaacgaaaaaaaatgtcgaaccaaaagcggatcaatccacaaagcaggataaca + tttccaattttgcagatgtagaggtatctgcgtcttcttatcctgaaaaacttgatgcag + aaacagatgatcaaataattgggaaggcgacgaattcgtcatcagttcatggaaataaag + agctgccagaccttgctgaggtgactacagcaaatagggtttctacaacatcggctgggg + accaacgtattgatacccaaagcgaatttttacgtgcagctgatgttgaaaacttaagtg + atgacgatgaacacagacagaatgaaagtagagtttttaacgatgacctctttggtgatt + ttattcctaagcattaccgtaataaacaggagaacattaacagctcgagtaatttgtttc + cagagggaaaggtgccccaagaaaagggcgtatcaaatgaaaacactaacatatctctca + aaactaatgaagatgcatctacattgacgcagaaactctctccacaagcgagtaaagtgc + tgacagaaaattctaatgaattaaaagataccaacaatgaagggaaggacgcaaaggaca + taaaattaggagatgattacagtgataaagaaacagcgaaagaaataactaaaccaaaaa + attttgttgaaggaataactgaacggaaagaaatattccccactattcctaggttagcgc + cgccagcttcaaaaattaactttcaaaggtcaccatcctatattgagctctttcaaggaa + tgagggtggttttagataagcatgatgcccattataactggaaacgcttggctagtcaag + tctccttaagtgagggactaaaagtcaatactgaggaagatgcggcaattataaataaaa + gtcaggatgatgccaaggcggaaagaatgactcaaatttctgaagtgattgagtatgaaa + tgcagcaacctatcccaacttatttgcctaaggcgcatctagatgactcgggtattgaaa + aaagtgatgacaaattcttcgaaattgaagaagaacttaaggaagaattgaagggcagca + aaacggtaatgaagatgtcggtaataataatccatccaattctattccaaaaatcgagaa + gcccccagcattcaaagttattagaacatcgcctgtgagaattatcggaaggcacttttg + aagacactagaaaatatgaaaatggctctccatctgatatttcgttcacttatgatactc + acaacaatgatgaacctgacaaaaggctgatggaattaaaatttccatcccaagatgaaa + ttccggatgacagattctatactccagcagaggaacccactgctgaatttccggtggaag + aacttccaaatactccgcgaagtattaacgttacaacttcaaataacaagagcacagacg + ataagttgagtagcggtaatattgatcaaaaacctaccgaactgttagatgatttagaat + tcagttcatttaatatagcatttggaaatacctccatgagtactgacaatatgaaaatat + catccgacttaagttcgaataaaaccgtgttaggaaatgctcagaaagttcaagagtctc + ctagtggaccattaatctatgttttgcctcaagtgagcacaaagcatgagaaagaggggt + tccttcgaaagaaacaaaaagacgagcccatttggtttcccctagcaaaattgactttgc + tgatctaagtaggagaactaaagcattgacgccagagcgtaatactgttcctttgaaaaa + caacgacagtagaaaatacaaatatactggagagggatctatcggtaatatgacaaatat + gctgttaactaaagatgcttcgtacgcgtacttaaaagattttgttgcgttgagtgacga + tgaagatgaagatgggaaacagaactgcgctgttggtggcccagagaaactgaaatttta + ttgagtgttttattgattttttttttctttttaatttaatgcaatgtatacatttacata + aaatatggacaaataagaaaaagggggaaaaacctcccacttaagtaaaactgaatatat + tagatacctttacctactatcaaaggtgaatgcttttggatcaataggggttccatcggg + ggcaataccattttggccgaactttctttccatatataatagtctagccttatcgaaatc + tacattatcgttttccataatttgtttaatctcatcttggagacatcatccaaaccacct + cttacgtcgttgctattctccgagataatatcgaaattcctggaatggagaccgttttct + aggtcatcagcaaaggaatcatttaaacgtatatgtctattaaaacgtaattgtattctt + gaacgcagtcacgaaatttgtcggctattacagctgttagtatttgatacggtttgcact + ttgcattttcaaatgcttaaatatactaccaactttggttttccggtgtgccataagaag + gcataaagacaatagcacaacccagaggagcaatttgagatagaacaaactcttcatact + cgtatttgcaatttttggaaattaattgtgtagcttttcttctcttaattggtggcagtt + tttatatattcagtttagaatcccttttttttttttttttttcttcgcttttcgcatctc + atcgctggaagtggtgcacttttacagggattaaataaggttattctttttggtcaaaat + gataaatataactattaaagttatttataacccgcccgcttccttattatttatacgtta + tcataaagtacacaggtcatggttcggttggtgcttaggaattaatcacgtctagcataa + gagatctgtaaactacaaccaccataattataattatttaatttttgaatacaaaaatct + gcatctactaaattttcatattctacaacagcaacaccagttggttgaccattttcctgt + ggtttcaattccgcgttattgattttgccgataggcccaaacaagtcgaataaatcgctt + cttgctgttgagaatggtaaattactacaataaatgaaacagtttctatcccctcctgga + ttgacaccttcagtaaattttgctgcagtttcatcaatgtggacagcggcatcctgcgca + agacctggttcagtacctctggtatcttcaaggtcctcacgcctttgattataacgatca + ttgttctttctcttgttgaaacgcccttctctaacttccaaaactctaccttctacttcc + atgccgttgaatgtatcgatagctctaatcatttcatcctcagtaggataaatgacagaa + ccgaatcctcttgaaaatccgttgaaatccaattctacatcggcacgcaagacatgacca + cattctttaaacatatcttttaaggattgccaattcatagagtacggtaaattgatgatg + aacacttcaaacccattatctatttcttccctagtagctttcttagaaaattccttggca + gcttcaggaggaggattatcctgtcttaccattagtttccggtccataaagagggcacca + tcaaacttcgatatggcatcttggacagattcgtttttggtaaattccacagtccccata + ccacggtgatggccctttgatgtgataatgtcagcctccacaacttcgcccactgtacca + aacaattccttaaggtcttcaggggtacaatcaaaagtcaagtttctcacgaagatacta + ttttcaaaatttctcttggtcgcgtcaaattgcctttctaagtctctttcaacaattgga + cctaaagtacgacccctaccgccaccgcgtccccctctgaatgatctgcttcctcctctg + ccacggcctcctcggcgagggggccagttcctatcatcgtggtagcggctgccaccataa + ctctgatcgtaccgatcattgaaacgggagcctcggtcgcgtctctgacgacgactgcca + ttaccattattactgctactatcgttataatcatcgtacctgtctctgtcgtcgctcaaa + cgacgacgtacaggtgatcttgatctactcctatcatttccatacatccctagctctctc + tccatcttggcgaaaaaatccagctgatgtttgtttccttttcgcccatagcttatttct + aatgggaaatcctcaatataattgatttcatccaagctttcatttttttttcaatttttt + tcaccttcaagtgaatacatcacttccgacgataccaaaataatgacaaaatgccatgag + aagatcttatgatatgtagtaaatgttaaccaccattgctatttccttgctaggtttgcc + aaagccgttggcgaaggcagcactagacggcgagtcacttccgtctctttgtccacttcc + tcttcaccatcaaatcgaagcctgcaagtgccgtccctcttggttccgatgacaattgca + ggataaaaagtggtcgtttcaggatatcttgccagaaccttagtacctggcgggtagttc + ttagtgggaaagcctggcgggatcaacagtagttctttccgattacatttatacaccttg + cctgagttgcccaattcatcaggttcaggatctctcacttcaaatcgtgtaccatctgct + acgactttgagtactcacactgtatcattcgccgtcgcactgccccttctgggcttatag + gcaacctctgagcccaccaaaataggtgcatttgggttgtattcgctggtccagtacgat + ctgccaactttccccacagctttcttgccctgcgacaatgccatgcgtgtcccttctcct + tcttctgcatcttcagtattttcctcactcttttcctctcgtgactgattcaacatcaac + gacaaattggaacgtacattgtcaaggattcggtgcactctgttgacgttttccatgtgt + tcttggaaagtgttgagatgcaattgcagctgatcctttgacatattaaggaaatttagt + ttttttgtttgcagctcatcatcaaagggaatgacctcgttggcattgtatatgtcttgc + aaggacgacactacaacatcccaatatccgtccataataagaaaggtgaccgtgtgtttt + gctgtgaaaaactccccccacttagagaagccaccaaggtattgtgtctttaaccttttt + cgtatctggcaaaatcgaagaggaaagcagaaaaccaaaagaaaaaaatagtttagtaac + gcgtcacctcgtacaaacgtacaatcatagcaacttatgagtaaataggctcatggttat + agatacgttatatagatgtatagaggagagtcccgagggcgatcgcaaggccgagagact + aacctaaccaggtggtagttgggaaatgtcgaccatttcgctgatcttttcgtcttcgtc + ggcagcttcctcggtgcttgtcttcaaaggagttcttggcaatgccatcataccgcttct + tgcacactctaggacaccgaatggctcgaccaacttcaagaaggcagagatacgtgtggg + ttttgcagacaattccacaatacagcttgtttcgctgatgtcgacgacacgacctccaaa + gttgttggtcaagttggtgatatcgttcaaatgctcgtgcttcaaccttaatacctcact + ggcgggcaagttggcagggtggaattgcttttctctgatttcggcgaccaattcttggga + gtcagcggcgccagcattggtggaagtgtggtggtgcaatagtaggtcttcgaagtattc + agtacccaatagagagattctggccatcactagctctcttttgatgatctcagaattggt + atagtctaggacggcgtagacggggaccaagtcttcgatttgtctgcgtgcttgttcgac + tacgccatcttgcccttgcaaaacaatggtcattctacttaggtctttgacctcggtgtt + gcacacgaccaacgaatcgatgttaaagcctctggcagctaacgtacccgagactctgga + caagacaccgggttcgttttgcaccaaacagttcaagacatgctgttttcttggttgacg + agaaggcgctggtgtttcgtaaatgatggatgaaacggcactgttggcattccaggaagg + agtgtctagtgtgggcaagggaggtcttgttgcgtgtctgtgcatctgcttgtacgccaa + cgcggaggtcgacgaggaactgcaacgcaccatggtagcacatgaagaagcaaccaccct + gcggtggccgctttgcaataacgatctcagcattagaaagactgggttagtgagctcaga + tgttctaaagattctgtatatacgaactatgtacttccatatataccattccccgttctt + gttaggtaaaggttaaaaaaatattttttatggcacttcggcgatgcgaaagaaagtgag + tcacgataacggtgaggtgattcgttggatgtgccatcgcacaaggaattgctggcgtgc + taattcacgaccttgcttgacaaaagtatcgaggggaatggtgccacggtgcagcatcct + agataagcactcaatggtgtccgtgagagcgtagtcctgggcaacgaggttgtatagttg + attgagtccatctgttttggccactgctatggagttcacgtcctcgtcatccacatgcga + agttgagctaaactgttggactttggccctatttgcggttcaggacgtctatctgtgcgt + ttagcgagtgcatggtctgctcaatagcctgctcaacggctcgtaaatggttcttgtcga + tggcgattatttcatggaatctggcaattgattcttgcatgacggtctgtctcgtcagta + tcttgtccgctacatagtccacatcctctctgtataactcattgactacggtttgtagat + tttgtagcatttcatgatggttggttggggagatatccgtgttgtccatgtccataaggt + cgagtgcgttagatgccggttgaggtggtggaggtggaggcaatggcggttttaggtgtg + gagacttgggctttgggggcagtgggggagtattctccctcctgctggagctgcgtattg + ggcttagggggtagactgggggcctgatcttgtggcggctcgtgtaagagactcattaat + tcttgtacgaccattataaggttcatcgcggcaggatcccagcagtggagtatgggcagc + gcaatccacccattggagtcgatgtattcttgaatgggcaaggaactactgatggtgttc + atgtcgaaattttccaagtttatacttataaagggaggttttaccgggtacatactgggt + acccacatgatcacaggaatactgtgaggagatgaaccatcctcaccagtactaatggta + ccatatatggacaacagtagttgtggagtgccgtcagagtgcgtaaatacccttgttctt + ggccttaaactgtgaaaattgtcagcagcgctaaagaatcgtggaaagttgttcttccgt + cgttataaatgggttgaatgaactttgaacagccaattgaccaccgcctgggaacagaga + tcttgccgtttgcagacatcgttgctcttcactctctctcttttcttggccgttaagata + ccaactgcccacaaaattttgccgtaactttttattctcgctctgtgaaaaaagactact + acattttttgaccttttatctatactttaagaaacaaaggtccaacgaataataaagaac + aaagagtaatgagtagtttgtatgtgtcataatctcttctctccgcttcccttttcttcc + ttcttcttctactaactctctgcgcgtccaatttagctatactgttgttggtgtattcat + agtggttagtgccatgtccgtgctgttctggataatggctccaaagaacaaccaagcgta + tgtgcaacattttttttacgatgtgtctccatattactagtttccctttcttttttacta + acaagagtttgtttgttttgttttgtcgtacagtgtttggagaagtacagtgattttgac + attggccatgatgtttttgatgtgggccattacgttcctttgccagctccatcccttggt + ggctccacgccgttcagatttgagacctgaatttgcagaataaagcgtcgagtatatcaa + gccaagtgacgtttcctgagtataagaaaaataagcaaaataagtatacataaaaataaa + tacgaatagaaatatagtggcatctaaacaacccagagattttttgaaaaacacaatgag + tatatactgatagacaaccacacggtctttgaaacgagacgccgtctacggttgtatcaa + aatgtgtaaacgggccatttcaaagcttgatcttctccataattagaacccatgtcccgt + ggaagcgttttaaaagacatgaggcagaaacaggagtgtgatatccttgcatgcgcaagt + tggtaatacactgcacaaaacacgcgtgtgcacatacacttatgtggttcaccgtgccgc + tgctgtgtttatctgttgctcgacatgtgctgctaatgattatgttggaatagacaaaca + atttaaagtaatttcttctttttttatagtgagaaatctcaaaagaaaaaaaaaaaataa + aaaaaactaaagagctgccgggtgagcaacgactaaaagaagaaaccatttagggcttca + tcagcaacttgtaggaggagaaagcagtatataactagccgcaatatgtttgtggtggat + tggagcgtgcagctatgcatgggagtgatttcacctctcttccgtgccctcgtgcaactg + cctttatcaattttcgtatggaatgggtttcagacttgtgcattgcctatcaacatccca + ttagttgttcctgggacgtcgctgagccgtttagttgcacaaacaagcacattagacttc + tatgtggttttgacgttgtttcaatattttgctgtgctttgtgcttttggcagcatcata + ggactcatctttggatttatattgggtgtgttccactcaatctgcggggtacccagtgta + tacataagtctagaatggaaacggtggtttgctccgatacgtacggtccttgaacgtgct + tccactagtattgtcaacattatgcgaggacaaactattgcgccaatacccatgcctaag + cccaatcccacgcatatatcaaagcctaacatgaaaaaattccatgatgagcctggagct + gatgatatgactataacgcatgatgtgaactgctacatcaccccttgccaaacgcctact + aacgaaaaaattcagcattataataatgattcattcaacacgaccaccacagacgatgaa + cccactgatatttgggataggtctgacacttaccaaaactcattcgtcaccaatgaaact + ttgatgtctctttctaatagagctaagcttcgaagaaatgccagtgatgcggacatcgtt + aatataaagattttacgaaaggaattctaggtgatattgcaattacttcttctcatgcac + taacaagtgaatgatagaaatatgttgagttgctaactgcctgattttaaataagtttca + tattataatcttttagcatatatatatatatattgatcctctctcttctttattttctgc + cagtaacccatgtgtgaagaagaaaacataaataaaaaagcagtagcacatggacacatt + cacgcccgaacacttctaaaaagcagcccacacaagaaagtagatataatgtaggacacc + cagcttgtccataattgctaatagcatactcaggataacatatattaatgacgactcgtt + tgctccaactcactcgtcctcattacagattattatccctacctctccagaaacccttca + atataaaaagggcagatgtccgctgcgaacccttctccatttggcaattatttgaacacc + atcactaagtccctacaacagaatttacaaacatgctttcatttccaagcaaaagaaatc + gatataatcgaatctccatctcagttttacgatctcttgaagacaaaaatacttaattca + caaaatagaatattcattgcgtctctgtatttaggcaaaagcgagactgagttggtggac + tgcatatcccaggcattgaccaagaaccccaagttgaaagtttcttttctacttgatggc + cttcgaggaacaagagaattgccttccgcctgttccgccactttattatcgtctttagta + gccaaatatgggtcagagagagtggattgccgattgtacaagacgcctgcttatcatggt + tggaaaaaagtcttggttcccaagagatttaatgaaggtttaggcttacaacattgaaaa + tatatgggtttgataacgaggtcattctttcgggagccaacctttcgaacgactatttca + ccaacagacaagatagatactatctctttaaatctcgaaacttctccaactattatttta + aattacatcaactcataagttccttcagttatcagattataaagccaatggtggatggta + gcatcaacatcatttggccagattcgaatcctactgttgaaccgacgaaaaataaaaggc + tgtttttaagggaagcatctcaattactagatggctttttaaagagttctaaacaaagcc + tcccgattactgccgtgggtcaattctccacattagtttacccaatttctcaattcactc + cactttttcccaaatataatgacaaatcgaccgaaaaaagaacaatattgtcattgcttt + ccactataacaagcaatgccatttcttggacgttcactgcaggatacttcaatattttgc + cagacatcaaagcaaaactgctggcaacgccggttgctgaggcaaatgtaataacagctt + ccccctttgcaaacggcttttaccaatcaaagggcgtctcatcaaatttacctggtgctt + acttgtacctgtcaaaaaaatttctacaagatgtatgtaggtacagacaagatcatgcta + ttaccattaagagaatggcaaagaggcgtagtaaataagccgaatggttggtcatatcac + gcaaaaggtactttggctttccgctcgtgataaaaatgatgctaacaattggaaaccctt + tatcacggttataggatcttcaaactatacgagaagggcgtattcattagatttgaatcg + aatgctctcattattacaagagatgaagagctaagaaaaaaaatgaaagcagagttagat + aatttattacaatatacaaaacctgtaactctagaagactttcaatcagacccagaaaga + catgttggcactggtgtaaagatagctacctccattttgggtaaaaaactttagattcta + tcatagtatgtcgcctgtaaatatggaaaaataaaagaaaaaatgataataataaatgca + ttatctaaaactccttaaatttgcaaacatcgcaaagccattaaggtagagaatgagctg + accttgttatagttttccttttggcaaccgtggtttccgtcctacgtaaacatactgaac + ttgacaaagtatgaccaataggtgacaaattgtatcaattttacccaaaagggcgttgaa + ttaaatggtatctcgtaaaacaatgcattggatcggtattaccagtctacctaataaatc + gatagattcagccaaaaatgatatcaatgcaaactttgatgataaacctactgttgagca + tcccatccagtttatactcatttgaggcacatacttgaaacatttaagcagatttgccat + tacccacaaataatttatgtgttccaaatagaaaacgccaaatttacctgaatttttcaa + cggtaaattagagatggaacagccatatgtaaatatgcctaatacaaggaacacacttat + aattattatggaagtaatggaaatgccctgataaatatgtttagttgacctataatagta + taactgccttaaaaccatcatgcagcaacaggaaacgcaaaaatcttttagaatgaggaa + actggatataggtatactacgggcatcattcaacggataaaaaagtgggaatctttgaga + cagttgttccctcaccagagatgaataacaataatgcaacgcgcaatacaagtacagacc + attaccaacaaaatctagcagaaataaatcatatgaaagcccgtaaatagatcgatgaag + tttattatagcgcttttgacatagaatggcgatgatctatgtaagaaatggaagttttct + cccttagttagtcaaacctttcatgtatactttctccttcaattatgcatactgataaaa + aacctgccaagccctgcagaacaataacaagcatgtgaatgattcaatataattgcttcg + cagggctcctcaacctctcctctggaaaaccttacctcaatgaggttaaaaattgtgggt + ttttcgttcgcagaggaaaaaaaaacttgtcaaaacacgcctctgcagtagaacacgaat + tatacataagaaaaataacgatacaagaaaggcgatttcgatttgggactaagtgttaca + cgaaaggcatagagtatcaaaaaaaggaagtcaagatggattacgatagctctgatacaa + tgaacggtggttcaagtaaccccttaatcactaagatgaatacaatgaaattattatatc + aacactatttggataaagtcactcctcacgctaaggagaggtgggctgtattgggtggtt + tgttatgtttgtttatggttcgtattacaatggccgaaggctggtatgtgatttgttatg + gtctaggtctatttttattgaatcaatttttagcctttttgaccccaaaattcgatatgt + ccttacagcaagatgaagaaaacaacgaattggaagctggagaaaaatcagaagaattcc + tttccattcatcagaagacttaccagagttcaaattctggtataacagcatttagagcca + ctgtcatttccctcttgttgtcggtattttcaatcttcagtattccagttatttttgccc + attctttatttgattgttattttcattattattgttttttttaactatgagaaggcagat + tcaagcatatgataaaatatagatatatacccttagatatcggtaagaagaaactattct + cattcttctaactgatataccctgactccacatacctattcaacgaacacattcgtatct + taattatatgtaaacaaaattatatgatagttacagaataattgtgattattttctcctt + ctactcatcttctcttatattggaagaatacatgaagttattgattaagcaaaattgaag + ggaagaggctcattttcttccatttataaatggagagcttgagatgcctccaattgataa + atttttgtaaagactttcaagaaacatgtctaaaaagaattgacagcttcgaacctttct + gcataattactaataccaaattagtgagaaatggggtatgtcgaggaataaaggatgcaa + aagagagagtaatgtcatgcaagttgtgagtttgaaatgttcacacgtttataatgtcat + actcgtggcgtgcatgtgttttagtgtattagtttcagttattaaagaacttagtaaatt + tatcatcgaacgcattttgattaagtggcacaatagacttcaatggaaaatgaaaatcat + atctgtagcaaaaacgacagcgaagataacggttacacaattatcacgttgattcgtcaa + cttaaagatgacctttttacaatttatcaataataatagacaagaaggacaaggttatat + ttcagaaaaattattcaaaactaagaagaatgagatgataagaaaaacagttaccaattt + agtagctgtaagattaaaaaacttatcccacgaatttgatgtaatagagaattatctgcg + ctatatagctagcacagtgaacatctatttactgctattaagcgccactttaacaaatgt + gccagaaaacttttgaaagaagcaatcgactccaaatcaaactcagaaactgctacggtg + gttctgcaagaaggattttctggcatttgcttattgaaagcgtcttcaattatattaaaa + ctaaaattgaagtttccaaaaaagaaagatagaactgatattagcaaattgtgtgacaag + aaagaacggatgacacagtggttagaaatttcaattttgatgaactgagaataataatat + tatgctcccctggattttatgcgaagacactgctgaaaaaatttctgatatatgcagaga + aggagcaaataagccttttaaggaaccgagataagctgttttcgcccattgttctagttc + tacaaaatatctacagggaatcgatgaggttgtaagaaatcctgaatactctatgatagt + gcataacactaaaaagttgaaagaatcccgtatcatggacgatttccttgaacatttgag + caaagacgataacaaagcatggtatggcgcggaagaaaccgagagagctgcaaaattaga + tgcaatagaaacactacttattacagatagtgtactaaaaaggaacgacgtgaaaaaacg + tgaaaaatacctagacctaatagagaatagtggaaacaacaatggcaaaatattcgtact + cagtacttcaaaaatcacagtgagcaacttgacaaaccaacagatataggctgtgtctta + aaatataccatccaataccttgatgaacttttcaaagatgactaaaataagtgaaatttc + aacattaacttcgaatttttttctttttatctaacacttgtcaaacagaatataaggatt + acttgtcttctttgctacatattgctaccacttctattacacaatagtttcaatagcttg + cagcgtagctaaactctaaaatttatctaaatcactcatataaaccgaacccttcccctt + ccgcttatagtacagtacctatacatttcataaacatggcatgcgatcagcgccaaacaa + tatggaaaatccacagaaagctattcattgaaaaaatagtacaaataagtcacatgatga + tatttgattttattatatttttaaaaaaagtaaaaaataaaaagtagtttatttttaaaa + aataaaatttaaaatattagtgtatttgatttccgaaagttaaaaaagaaatagtaagaa + atatatatttcattgaatggatatatgaaacgtttactggtggaagttttgctcatatat + tattattcaatagaagtaataaagaaaaagttggtaaagcaacttaacagtaaaaaggta + atgattgaaaaagtttttgaacatctaagctatatgttgatgggtttacaattttaccat + tagtactcatgcctatacttttctgttcgtccttaatgtccgcgatttagagcaatcatt + gaaagtactagatacattttagccagagaggactcgttgacgtagaattaaaattcaaat + gaatttccgccccattcatataccccaaataacaaacatattaaaacttcataattattc + aaaatgtggagtagtatagaagagcagtaccttcaaaattgatttcttcagtttcccacc + cgggatccacttgtcatgcggtgagaatcgtatattgcgtataatccgtgtttcatcacc + cacattatagtacaaacctactggtgtaaccattatcatattcatgacttgtagttaaaa + atctttcacgataaattgaggaaaaaatcacgttaaatagtttcatatcattctttagtt + aaagtctgtgcataaaaagaggatcgggtgttgtactcgttctatcaaaatattgttatt + gttgcattatcacacgaattacaatcaatttttttatctacaggccagagtacctgaaat + aatggaattagattcaaaaaggatagcttaatcggtctttatactaattattttacgccc + agaacgaaacataacgtgcattaaatctcactgtcacaacattgtatataatggtagcat + cgtactaactattggcattcattcaatattatacaattatcttattaatactttctcaag + tagtcttgaagaccagcatgtaggaaggtgatgatatgctccgtaaataattggaaatat + tgagatataataacgatgacaatactaacaaaagtaataggtgagctccgccaattgatt + gttttgttttgaatatatattgatgcttaacaatttagtcgtcaaatagcacctgcaaaa + cctacctcttacaggctttttttcaatttactccccatgtgggagttctacgtaaaccac + ttagaatgcagtttctcttctaaataacatgttatatcttctgaagaaattgaaacatat + tgattcttatggtgagagcaatttatcaatgttttatcttaagttctactgcccttggaa + attatgtcctttgcttcccattttatatggaagaaacaaatcagtacaaaatgcttggtc + atgtcataagcttcatcgatttattgacttcccaactccttcggctaatatgaatttttt + tttctttataagagtttgctatggaactgtctcgctgtttatttcaacgtttctaaagaa + aatatccttttttatttatgtgaacttcactgtatacttttagtacctttcggtggattt + cttctattttactcgatcacttttcttgactgtgcactgtcggtacaatccttattgtat + taacttgcgaagaatatttagtcgttcagtagggttcttttggaagaaatgctagcttaa + catgagtgtgatctcttctagaattaactcttttgtttatccatgtaatctgttaccaac + acataaaatgcattgatagaccaaatatcaaaagcgtgcaggcatctcttatctaacagt + aagcaaaattttgcctgcgctttacttcaattttccattctttttctgagtcttcgtcag + tgcatatttttttactgacagcaccattaatcaatcagctcacctgcgcatttccaataa + tcgtgctcatgaagatcataaagaaacgtccaaacatgaaagtgctcctttagaatttct + aaatccaaacgatatatgtacccctcgttgatttgtcctaaaatgagtatagcttgattt + tcgttatatattttttcatagtattcagaagaggagagaggatgtgatttatcaatatct + gcattaagtaatgaactttgccctctactgaggatattcccactagaaaactataaaaaa + tgggagaaagtcaatcgctgttcaggattcaacatagttgttgacaacgtatgataagaa + ggccagtccagtcctcctaagttaggttaagggaggtagggaaaaactgaagtgtaatga + taatcgaggatgaaagtgtgcggccagaaaaatcttttctttttatattaatctgtaata + aatacaagacctatatattaaagtaaaacaacattgaatcagatcgcatactggtgtaga + cgtgaagtagacatatgataatcgagcagggtgctcaatttggtgtgggacacgtttggg + aaggttaaaagaagaggtatatccggattacatttatttttggcgtattttaataattta + ctaaaagcgcgaatagcttcccattagcaacgctcagggactctttctaatgtttatagt + aatactttaataagatgagtaccaattgcttctctatgaggcacaatccctaaccaaact + tttatttagaattaccggtaaactccgttttcttcatagattaaatatttggctaagaca + acaaaatgttctttcattttaccaaacgagaaggtgatagctgtacgccagaaaagaagg + taaaaactctcgccaatccagcaatacctgtacctctagttttcgatcgcattatgtcat + tatgtaaaaccaaggagagaagcaagttacggtgttttcatatagaataatttgagaatt + cttaataacataagatatataatcaccaccattcttatgagattcaacgttgaatggcgt + tgctagacatatgagctgaggaacgactattcgcattttctttcaaggcaattaattgtc + ttgctctgaacccttcataatgaatgtaggaagttgtttcgattaagtcttggagatgag + ttcgaatcaaaaattcccttaaatatacaaaatcacactggttgatatcctcaacattaa + tagcgctccaacgagtttttcttcccctgaaggtttcaccgtttatttcaatctcattct + cagaaccaaccactgcaaacggaatgatagatctaacacttctgtttagttctaattcct + cgtcagttagttcttccgaatcataaggataaatcttgaaattgtatttttcgaattcat + tttgaataagctccctaaactccgttctttcatctaaagtcaatgtatccgacttgccaa + taactggtataacatttgctatttctgtcaatcttttcaaggcttcaacgtcaaggcggc + tcaactcctttccatttggttgcaggaaataaagaattgcatgaactcttgtatcagtaa + taaacctttcacgttgggctgtcaattctttacgtaagtattgagaatgttgttccttaa + tgtacttcacaataggctcccaagctttagaattgtcaataaagtcaccaaatccaggtg + tatctataacattaatattcaagcgaacgcggtcctccacaagagtatgagtagaaattt + tcatttcagttgtttttgtaacaggcagggcagaaatatcatcaccagtagcagaatcaa + tcaaatgtgaggcaaataacgtatttattagagtacttttacccaatccggattggccaa + caaccattatattaaattgaaaacctttcttcaacagacgatgttcgatctgattcgtga + tggtatcaaaaccaacataagaagcaggctgtactgagctgagaggatccattaatatat + ttatagagtgcttgtagtaaccgtggggcttggccttgtattttcttgtctttgcctgaa + aaaagttaagtattactgatacatgtttacatatattctattcagcgattctgatctatt + tgtggtctctcgaggaaatctcatcgtatcatacgattatttccgcaagcattcagaaag + caaatcttaatataaggcacaatgttcccaaataataccttaacttggagacaaaaacac + cagggaatatcagcaaacaaactatgaattctttgatttttggtaaacaattagcatttc + acaaaattgtgcctaccactgcaattgggtggttggtaccgctaggaaatccttcactgc + agattccaggccaaaaacaactgggatctatccaccgttggttgagagaaaagctacaac + aagatcataaggacactgaagataaagattttttctctaataatggtattctactagcag + ttcctaaaaaaaaagtatcacaccaaaaaaaaaggcaaaaactttacggtccaggtaaga + agcaattgaagatgattcaccatttgaataagtgcccatcatgcggccattataagagag + ccaatacactgtgtatgtattgtgttggacaaataagtcatatatggaaaacgcataccg + ctaaagaagaaattaagccgagacaagaggaggaactttccgaactagaccaaagagtcc + tatatcctggtagaagagataccaaatataccaaggatttgaaagataaagataactatt + tggaacgtcgcgttcggactttaaaaaaggactaggctcatctttcatctagtggtttcc + tttttaaataaacttaagcctgtatatagaaacttaatttcaaactgtatatttattgtt + taagacagcatcaatggtagaatacaaggagcggaaacagaaagaaaagtcgaaatagaa + tttttcgcttttggcttaacaaaatgaggtctttgaaatcaagagttttgcttgacaaaa + aacaactatatttcaaaatattaggaaatgatattattagtacaaggacttacagaaata + tacttttaatataaaaaggaaaaaataaaataaaaagagttaattctttattaattttcc + aacgtcctacattgatatatctgctgagatagaattaatgttagtaagaactaaatatta + gacgcgaactgttgtttcttacatgacagtacagcaggataataatcctttgtcctcctt + cttttctgcagttgtagctgctgcaggtttagtttgtctcttagccgcatcagaagtagt + ggtcttcttttcagtggagggcttggcttcttttgcagggaaaagttttttggcggtttc + gtagaatgttttaccttgaatttcagcaattctcaattcaagtggagacgcagttcttga + gccgtcaggtcctgcaagggtaccagcaccccatggagagccaccgtgtacctcttctat + actggctaactcagcaaatgaattcttataacccagtggtaaaaagataattccgtgatg + agctaaataagacaaacaggctttaacggtactttcttgaccacctccgtaactggaagt + actaacgaatatccccgcagctttgccgttcaaagagcccttggcccataatccaccggt + tttatcccaaaaggcggaccattgagccggcaaattaccaaacctagttggaacaccgaa + caaaaaggcgtcatattcgagcaacgttttctcagtggcaacaggaatatcttcaggttt + ctgaggagcgttcatcttggtgaggacttcatcaggtaaagtttcctcgaccctgtatat + atcagctttaccaccagctgcctccacacctttcttaacagcttgggctaaaacgtctat + gtgcccgtaggtagagtaagtaattatcgcaatctttaccattcttatagtgcttcaatt + gagtctgtattgttttgtttactctctttttttttattgatactaacctgacccctcttt + tcctttttcctcttcttgttttctttctgataaagtttacaaacttcaaataaaaatgaa + cgtaaaacaagtaaaaatgtaggatgttatagtatcaactagctgccggaatagtgcaaa + ttgtatgaatcgaaataagtacgctagccaaggcagtaaggcctaatgcccttaggcaaa + tttattatttatattaagttccaagaaggggaagcgccttctttccccgcagtacagtat + tttacccggaggttcttaataagttattactaaaagggtcataacggatttattaataat + gggattataaaaataatgcgtgtgtacctagttatcgcgtgatagcttccgcaattttcc + aaccattagtaaattttatgtaactaaaacgaggaaggaaatagtaacgttcattttctt + aattataaatatcattatcatagtgtatggttaaaagaatatattattgtattaaataag + tgctaaatactaaatggtcatgaggaaagaaaaatatgcagaggggtgtaaaagtaggat + gtaatccaactatagtttgctttcaatgtttttgaccaattccttgtatttctcagtaga + ataggactttggcctttcaatggaagcaccgatggccctatcagtgatcaattgagcaag + aataccaaatgcccttgaaacgccaaataaaacggtatagaaagaagattcttttagtcc + ataatattgtaataagacaccagagtgagcatctacatttggccatggatttttagtttt + accatgttcagtcaatacgccaggtgctacctcgtatattgatgaaactaacttgaataa + ttcataatctggaaaatggtccatggcaaacttacgctgagccatataacgaggatcagt + tttccttagcacagcatgaccataaccgggaatgactcttcctgagtttagagtatccca + taaatatttttcgatcgtatctttagagtagtcatcatttacctcttctttaagtgcaaa + taaccattctagtacttcttgattagcacgcccatgaagtgggccagccaacccgttcaa + acctgatgcaagggacagataaggtgatgatagtgctgagcccacaagatgggatgtatg + tgcagatacattaccaccttcgtgatccgaatgaatggttaaataaagtctcatcaagtc + cacgaaatcttcatccttagaaccaatcaagttgaccagatttttagcataatcggcatt + tgggtccacttcacccattttgccatctttgaatacattacgataaattttagctgcaat + aactggcaatttacccagcaagtctagtgaatcttcaaaagtataactccaataatcttg + cttggaaattccttgagcataagccttagcaaactttgactcgctttccaaggcagttac + agcaatagagaattgagccattgggtgtaagtcctttggtaaattatccaaaagttgaac + gacatgactaggtagttccgatcttgacattagatcagctgataagttttcaacttgcgc + ttgagttggaacctcgccagttagcaataaccaaaagagagcttctggtagtggttgtga + gcttccttttgccttgggcaggtccttttgaatgtcggcgatcgtacgacctctgaaacg + aataccgtcttctgggtccaaaacggaaccttcccatacgctccctggaatacctctcat + accaccatatacctgttctaatagaacatcgctaattttagttttgccatgctctttaac + gaattgccttacatcttgagcatggatggggtagatttcgctaaatctctcttttagagt + cttttcttggcttgaatttgattgtaaatatgatgcaacatttctgtttgaatttagata + aggaactgtcatttttcttgttactagtattattaaaacaaaaagttttgagaacctgtt + atgatatgtgttgatattgttccctgaaacttaataatgaggaacgaacaccatatctaa + agaaccaagttttaatagtcttatatacgctcaacgtcgagaataatgggcccttttgag + agcatgactatctttagattaacgtcaccaatttcatacgaatgtaacgctcaaaaggta + attgcgcttaccattgttattgtttttccgctgtgcctcccattgctgacgccctgatag + acgtctagctacggaaaaggtcacactttttttctgcaagaaaaaataggtcgtgacctc + ttcgcccctagtcgatgaccaccggataaatgcgtcgaatgtgcgcgctattagtttgcg + ctcttttgagataccgccaagccattgattgaatgacccaaggacggatttgttcaacac + tccaaagcggcttatgttgtggcaacaactctggggaaagtcagcgcaatgttttcccct + aaggattcgcgcgagccgtgtgtaacaaagttccgccggtatagcatatctaaaccacag + aaggacgcttatacggccgtcttccacagtaattagaaggacattagtcggagtgtaaat + aagacggttgcatacgcgtatgaacatgggccattcattttacggaaatggcaacacatt + ttcttgtttttaatgtaaatagtacggagttcctttttggtgcgctgaatgtatgccgta + gtttggtttcttgccaggcgtgagaagagaggtcacacatgctgttggggttcatgattt + tcagccttcgctctgctaagtaaaaggtccattgatccgaagcgcttcctccaatatcgc + agtgataaacggtcatgtaagcctacagtctttaaactgtagatgatttaatggattatc + tcatacattgaatatcacacttcagcagttcaagttttcattaacattggtcttctccag + cttactctgtaaagtccgctgcagttttctagaaatcataatctgtacctccattcagcc + atctgaggaacctccgaaataacgagtaaatgttcaaacatgccatgcgccttggtttat + tgtacgagttttagcatattgcaaaaaaaaactgctcctgagtacacttgttttacgtcg + gaaagataaatgccgtaccatttctgccagtagcgacaccacacattgtaaaaacaatgt + ggggcgagccgggactcgaacccgggacctcccgcaccctaagcgagaatcatacctcta + gaccacacgccctcctttgaaaaatcccggcagaacagcgcctgaagtctgggatacagc + cctattttccctgccatcgtcattgacctttttcttaacgaccagattaacagccagtta + ttgaaagtttgcgaacgtgaagtaaaactgcaaaatggtcgttttaagtcaaaagtaacc + ctgcgtcacacatgagaaacattatcgctaagttgttgttactacttcttgtaattaact + tactgtcgcattccaaatggactgcgaaacagacacgaaacaattacgaaagtgacgaca + gagtaaatattgtcattaaagagaatgatgacctccgggtaaactgtgagaataagtcta + gaccattcgttaaacttcatctttaattgagaagcatagtaaaaaataattaacaagtta + acacaactatcttgacccatgctaccaaggacataagtaatatcctccaccacttttact + caaagtatatctcattctgagaaatgggtgaatgttgagataattgttgggattccattg + ttgataaaggctataatattaggtatacagaatatactagaagttctcctcgagggtcta + ggaatccccataatggaatctatatttctatataccaatattacgattattcctcattcc + gttttatatgtttcattatcctattacattatcaatccttacacttcagcttcctctaac + ttcgatgacagcttctcataacttatgtcatcatcttaacaccgtatatgataatatatt + gataatataactattagttgatagacgatagtggatttttattccaacacattcgagcta + gtataaaattgtgaggaattatcgggcatcttgtttattaggtacgcaaattgtaataat + tgccaagttgtgctttttagttgagaagtattccgtcctcatgaaatggctaatataagt + atggatttcataaatattatccaaagtaattatacacacttgttttttgctatgtcttgg + gtttaagagaaaatgctacatagaccgtaagaacgtcttcaagaatgcaaggatgctgca + agcgaagttgttcccgttatgaagcagaaagggcacatcaacatggttcaaagatttaaa + tttcggcacttgttcttgttcactatatgttttaagattcttatcgtgcaagtattcaat + tatatcctctaagtgaccgaacaatcaacacatcaagatttttgtagttttgaagttcca + caacctacaggttagtgctatttggtctaatttatacatgggaactatgtgaaacagtaa + tgttagcacaatggacctctcaaagcctaagaaaattttgctggggacaaaaagtggttc + ttgatggtaaagaagtagtttagcttcataataatttctttgtaaaatttttctcgtagc + acactaagctttcagaaatcaaataaggcagaggaagtggttttgtcgtattcaatgact + aattgtaagactcgagtataacttatgcagtttccaagaagtaagcaaacagtttctctc + aacctttggtgacacactatgcgcatgcaagtcgtaatgcttctctatttatgtgtgggt + ttattaaaatagttagaattatctaggtttgtgggaatttggccatgaatattgaatagt + aacaagaacaaagcgtgaaaatgttgaatataatgaaagctgaagacatcaaatcatgtt + ctccaaatctttctgtctcgtatattttctttgatatttgttttatgcggaaattgcagt + ggaaacaatcgcgaaacattcgagaaagaaactaggtagctacaaaagtatggaaaaaag + ggtaataagcattcctttttttttagccgcaaagcctgcttaaccatacatgaagcactt + taacatatagatgctgctcttcactgttacattatatacagaaaaaagtgctcgcaataa + gaaacaaaccttcagaaaaagaagcttatcttgagcacaagtcattcacagcaaaatttc + ttttttcagttgctacatgttaattatggagtcctgagagtaagtaactagccattagaa + ttaatttccttcccttaaaaatgatagggaatgcacgacaatacgttaatgtgcacccaa + cttgcaccacagcatattctatcctttagtttgtacaatattattagctgtgagaaagcc + ccttcgtgtggcacaaatttattaatctctgtcaaaatacttctacttgtttatactgta + taaacgattaagttccattatgaattgtgaatttagaatcggtttggtacatcgctcagt + ataagtgctaaataaaattatcaagatttatatttcatggtatatcggcgtcaggatatt + gctttctccaatattcctgttgtgcttgtttctcaacttctactgctttaacaaagtatg + cttcgaatattgggtcagaactgaaactaaagcattttattacctcatttgaagcctcat + ttgctggtgcatctatctgcgtacttgtggcgtcctttaccgatgaatttacattcacac + ctgggggtttttctttcacaagaaatctgaagaatttgtagcatctttcactactataaa + agtaatagcgagtactccataaaccattatcaagtaaatattgattcatattgtctgtga + ttgctctccattcttttccctttaccgctggcctgtgggtgataacttccagcaaaagct + taattctgaaaccctcatccgagatatacacattaaacggaatcaacgtagacaaaaaca + aaatagaagtgacgaaaattgaaaaaccaaaaacgacatcacaagctttaacattatgaa + agatcacaactattaaaattgaaactgaaagtgccaagaaggaaaatatcatcggtttgt + aatgagccatctcgtacagcaagtaggtcatatatgatttaaaggtgtcttcaggtaaag + tgatattttctgcggatgataacacattttgttcccctaatgtatccaatttgacatcgt + cattattactgttgttgtctaaatgaggctgcatgcagatacggagttttgtttcttgtc + ttgatatggtatagacaaccgactggtgtatataaacagaacacagacttacattttcag + gttcgaagttttattgaagttcataaactctttttaaattattttgtaaacttgagctat + tcaagcagtattcttatttaacctaagcatagccccggatacaaggcgtataatacccaa + ctaacttaaaatgagagccacttactgaacaccttggaaggtcattgtttggttgaaagg + aatattattaactaagccttagaacaaggcgtcacctaaatgataatggcttttcttctg + ggcgcggtcggggcttcgactcatttgatactaggttaattacccttttacgcataccta + aatatttgtaactttagatctgtccactaaaagcgtttcgatgacttagttgtgctacta + atagtaaactctttgtgcaggcaaattatatcatcgctggctagactttttgtcaaaagt + tctgtttatacagtgggcagctagagtgcttaataacaaagatattgtcctttagactta + cacaagagcagaagcaaagataagcactgtttgttctgcttccatcgtttttgctgaaaa + ttttgcatgcatatccaaccaatatttaacacctggattatctcggttcaaagactacaa + taaacgccccaataattgtgatgcttttttcaatccagcccgcacaaatgaataccgctg + ctattggatgccatcttcagatgtacgtccaaactgtggttggtatagtttttagaacct + cgtggtgaggagtgttccattctttcaaaggcaatgagaaaaaaaaacagttgcgatatt + agtcaactgttttgcatggtttctttgacgcagttgtaaaatcatactcgaagggtagtt + ggaaaattttcaacatatattagtaatactttttctgtaaaagtgacacaagataaaatg + actccatggccaagttggttaaggcgtgcgactgttaatcgcaagatcgtgagttcaacc + ctcactggggtcgttaatttttcttttctttttttttttttatctccgacggagaaaaaa + ttttttctcaacccccggcatggcgaatggaaaaatcacctggcacgtcatttcatcaaa + tcaacatcggcctccccgtgctaatatgttaacaggccgagctcttgccatctcacgcat + tttccgctatacaaatagtatatataatattacagcttttaaagagggaagaggaaacgg + agaagaaaatatactactttatagagatattcggcaattttttttagcaaaagtttatta + aaccctaatatagacctttacttggagtttcgattttaagaattttttatcatttttttt + ttttgacgatttctgtcggtttcgttataacctgttgtgttgttgttgttgttgttattg + ctgggttgttgttattcactttaacattatcgaataaaatttggactttcaaagtcctgt + tcaataagttgtactaagcctttaagcgtttttaagttacaaataaaaactggaaagctt + aaggtagctttctaatattaggctctcaacctttttttactctccatttaccgctagata + ttaactagtagtaagtattgtgactactattccctgtaatcaaaaaaaaagtaatcagat + tttattttatttcgacattacccctcaaatatatgactggtatgaatgataataatgccg + ctattcctcagcaaactccaaggaaacatgcgctatcttctaaagttatgcaacttttta + gaagcggttcaagatcatctaggcagggaaaggcctcatcgaatatccagccaccttcta + atataaacacaaacgttccatcggcgtctaaatcagccaaatttggtttacataccccaa + ccactgctactcctagggtagtttctaatccttctaatactgcaggtgtgagtaaaccgg + gcatgtatatgcccgaatattaccagtcggcatcaccatcgcactctagttcatccgcat + cattaaacaaccatattgatattaacacctctaagtcatcatcagctgcttctttaactt + cgtcagtatcagctttatccttatcacccacatcagccataaatattagctccaaaagtt + tgagcccaaagttctctcatcatagtaacagcaatactgctattacacccgcgcctactc + ccactgcttcaaatattaataatgtaaataagataaccaatacaagtgcacctatttgtg + ggaggtttcttgtgcataaagatggtacccatgaacatcacttaaaaaatgctaagagac + aagaaaagctaagcacaatgattaaaaacatggttggtgcgagcaaattacgtggtgagg + caaaatctgctgtccctgatataataatggatccaaagacgactttaaaatccaacaaga + atcctcctactctttttgcaggcttcatgaagcaggtcgtggatatggatgataaatatc + cagaaggcgctcccacaagtggcgctttaaattgtcctgaaagggatatatacaggtcag + atcaaaaagattccaaaaataatacgcataatatcactactactaaaaaagataggcaat + gttttgccgaaaagtatggtcgctgtcaagaagtccttggtaaaggtgcttttggtgtag + taagaatatgtcaaaagaaaaatgtttcttctcaagatggtaataaaagtgaaaagcttt + atgcagtgaaagagttcaagcgtagaacatccgaatcagcagaaaagtattctaagaggt + tgacttctgaattttgcatttcttcttcattacaccatacaaatattgttactacactag + atcttttccaagatgccaaaggcgagtactgtgaagtaatggaatattgtgcaggtggcg + atctattcactttggtcgttgccgccggaaaattagaatatatggaagcagattgtttct + tcaagcagcttattagaggtgttgtttatatgcatgaaatgggtgtttgtcatagagatt + tgaagcctgagaacttactgcttacgcacgatggtgtgctaaaaattacagactttggta + acagcgaatgtttcaagatggcatgggaaaaaaatattcaccttagtggaggcgtttgcg + gttcatcgccgtacatcgccccagaggaatatatcaaagaagagtttgatccaagacccg + tagatatatgggcatgtggtgtcatttatatggcaatgagaactggtagacaattgtgga + gttctgctgaaaaagacgatccattttatatgaattatttaaaaggacgtaaggaaaagg + gaggctatgagccaatcgaaagtttaaaaagagccaggtgtaggaatgttatatattcga + tgttagatcccgttccgtacagaagaattaacgggaaacaaattttgaacagtgaatggg + gaagggagataaaatgctgccataatgggcgcgcattgaaataaacgagtacttcacttt + caaatatcacgatattttggaacaattcggtattttttacttaatctagtacactaagga + atgctttgttatccggcattcgtatcttattcctcgcttctattgttctacttttatatc + ccgtttggctgattacggatcacgttcaaattggtaaatcccattaattaaaaaagaatt + gtaaccttatttaaaaaaaaaaatagtacataacaataaaaaaaaaaaagataataattt + tgaatttattgctagacattcttacgtttattgtgttcatgttaacaaaaacacatgccc + gcggttattatcgctttttaattgctttcttaaaaagagtcggttggtcagatagagcag + atagtaaattatagtgctatttaggtctgttaatttaaatgaaagaaacataaatgaccg + taaaaaactaaaggcaaaagcattaatttattttatcccgagcgcacaaatatctaggct + tgtcatttgtcctaatagttcttcgattttagtgtctaataacccattggcatagtcgtc + tcttgattgttggtccaaatattgttgaatctgcgctaaacgagtgtaaccatcagtaca + gaaccttagctgaatcttgattaaagcttcaaaacttgggtcaaagtaaggtactcttaa + tgaaactaactgtggtagttcagttttcaattggttattaagattttcgaaaatatcttt + agctaagctcaattctttttcagcccttggcagtttagaggcatctttagcaggtttgtc + cactaatctacgaacttttgccttcgcagcatcgaagtcttgtttcttatggtctctctt + ttttatggcctcctcaatttctttgaaatacgtcgaaaactttgttattggatctagtac + ggtttctcttaagggcccgtctaattgcttaacagtttcgctatcaaaatcttgaacaca + ttgcaaataatagttaccaacgttgtaaccaccaccagcaacatattttgaatcgtcata + gaggttagagatgacctcggcaatggtagtctgtgatgctgtcacagctctcaatgagtc + caagaaacctttggcttccttttgtaatgcctcacctgctctttgaagaactttataacg + acgttcttccatgtcatactctttatcaatggtcttgtcgacattcttaattatcacact + gtgaccagctctgttgatagctttcttaaaaccttcccaactcatcttgtatcagctttc + ttctggcttcttataataggatttgtatagttttctatatcacagcgtttccttttcttc + tctcacgtctgcctgttattaaatttgttttccctttcttttaattcggattctcataat + tgttcaaaatccgcgcatttggatcgtccagcccctgcagcgcccagttcagccaacaaa + aattcatagggacgtaaaggaaacgtttcatgtctgttacaatgaaaaagacaagtcgaa + tcacattatgaatggcaaacgatagacctttcgttgcctttcaattacatgatattcgtg + gcaacatcttctccgctaacctaaaagttttatcagaaacatatatcttattttttctga + agtaaaatcaaagtgcagggaagacaagtaaaggagtcagcaaaaaagtgaagtaaactt + atatgttaactgtttataaccgtaccttgcttacatcatctatattttcgtaatcatttt + tgtacattgtttatatcttgttttgaagttttagctttgaaaaacatttaattgcgaagt + aacataatgttattaatagtgtacgactgaaacagccattctatttaaacatataaaagt + acgattagttggtgtggttatataagtcataaaataattatcatattaaaaaattgaaaa + ataaaaaactactcttttttatttcaatagttctcgttattagtaggtcgtgctcttaaa + agattaccctttcagtagatggtaatgggaatggacgagccagtacatatgaattctgct + ttgtagcaacacctgcatatgcgttgtaccaagcaatgaaagcaacaacaactcccagga + caccaccagctcttgtgacaccaagtctattagcaaagtgaccaatagacaacagtagga + aagttaatgctagtaagaagaacaacaaaaagaacataacagtggatttcatggtacaaa + cggttaaaccaaacgtaaagatggcccaccccaacaaataaaatcctaaagcattattca + aatcagattcattgtcttcgtaagcttccaagataccaaaccaaggaatgtaaattgcag + cgaaactcaaccaaaacccaccgtaagaacataatgcggtaccaccaaaagtattttcca + aagctatctcccaaataccagcaatcaattgcaccaaaccaccataaaacatagcacaac + cgacgacaacattaggaacagtgatcccttgcgctctcgcattgaacatggacagcacaa + atgtcgtcaacgcgaaggctgaaagacctaagggcgcaggattagcaaatttgtgcactg + gagcaggagctaaccctggattcaaggtaccaccaaaggcttggtataagtcgctcttca + aaaacttttgacgcccaatatagatatattcattgttatcacctccagtgtaaatcttgc + ccaacgaatcatgagatggacgttcatcttctgcaacgccattaacgtcgttatcatggg + aactatagtatcctgctggtgcattctccaaatctgtgtttccgctcgtttgttccttgt + cagacatattatttgtttgtatatgagttgttttgtggttgtttagttatatctgtgtta + gctgtcgactatttgcctttttcttataaacagatgctagtagtttctgttgtattgtta + ttgctattggacgatatataaaatgaacctatagcaagatctcagggaaatacgatccaa + ccaaggtgctttatatacagcttggtgtctctgacaggttggagtacagaggagaagcgc + catagccgcccaacccgcgataacgaaaaaaagctgtaagaaaatgcagtcttccccaac + gtttgttaccccaacaataccgaaatagccgcccaaccgcaaatctggcaaaaaatcttt + tcgtatctcgctgcagatgaccttccaacccgcccctttttgtagtcacgcctttaaggg + cccttgtttggcttgctcacatggggctacacgtaaaaaccgtagcttggagaaccactc + agccgctcgcctattccacggagctccggatcaacgggcagtggaccctctcgaaaataa + ggaaagttgcggggccaaaaattattatttcttatgtaaaagaagagactatatacgacg + cctatctacttttgttccacaactatccacttaagggctagataccccattattaaaaac + accacattaaacagagccaggatcttgatgtcaaatacaaggttttggacaacaaatcca + aatgtgctcaagatagtagcgcctggaacttcaatatttaagccgtactttctctctttc + agcatcaatgttttgacctcattgatcaataaagattcgtaggcgtagtaaaacacagag + aagtttttcaggtacttgaaggccacgtttgtaatattcttagtattgataaatagtccg + ctaaacagtagtgagcccaaaagcaccagcacgcttaatattatggagttattcaagtct + tcaaaaattatgccgatggttaggatttccaacgatatcccaaggttaaacagtataagg + attccaatacatttaaaaaaagcattgtctttcatgtttaaaccagtcattgggtaaaca + atcaatgacaagagtataggtggtacaacacgtagcgggaccacttcgctcattatctta + ctaatgtagtatgcaagtggcgagtaatagttattggatctttcttttatgaaaatgatc + ctttccagagcgaacgagctgagacctgtgaatgtaacaaaaccgaagtacgttagtata + aagaagaacagccccattctgttctgaaaaccgctgatatcattggagacgttgtaatat + agtgttcccaagaataaactcaataggatcgtcagtaaataattacccaataatagttta + gggtttctgtacatgtttttaaaacttcttgaattcaatatcgataactgttgcagaaaa + ccagcagattgttgacctgtgggtaaatctccattcaaaacgttactttcctcatcacct + tcgcttaaaacttcctcgatctcctgtgaaagctctgcataatagacgctatctttatat + ttatcgtgtagtagtttggtatttaagtcaatctcagtagcacctcgccttccatctgtt + ccctctacatcttcttcatctctgagtaaagatctgatctcatctctatgagctgcaaga + tgagcccactctctctgtgttgtaccatcactgctagtaaatgttgtttggtgtatcgta + ttatcaatatcgttagtatccgtaccagcttctaaatcggaaatgtttctgattcttctc + cttttcccctgaggaccggcttcaaaagtaatatcaatcaaataatcagcaatattatag + ttgtccggacagatatatccctcatttctcaaaaattctgacacttttttggcatttccg + gaatagaccatctcacctttacttaacaggaccaatttatcgaataaataaaatatattt + gatcttggctgatgaatagatagcaccaatgtcctgttatagtcgctggataaccttacc + aaacattcaataacattattggcattactagcatctaaaccagatgtaggttcatccaaa + aataaaaccaatggagatgtcactaattcacatgcaatggaaactcggcgtttttcacct + ccactaataccacgatcaaattcattaccaataatacgatctttgatatcaataattctt + agttcttccaacaccttataaactcttgccttcttggcctcgaatgacaatgcttttggc + aatcttaacagcgcactatttaatacggtttcaaaaacagtcaaagtgggcagcaaaaag + tcatcttgatcgacgaacccgattattttcgagaaagatttacggtccatactaataccg + ttaacttttatggaacccgaaacgtgacctgttttccgtttcattgctaggatatctaat + aaagtagttttacccgcaccagatccacccatgatagctaatatttggccgggcttcacg + ataccacttatttcattcagcacagtttcttcaacaccatctgaatttatcgaggggaca + ctataagtgatattttcaaaacttaatgtcgccagtgtgtcatcttcattttgtaagaaa + ttattcaccgcatcttcatctggcaaacgaatgggagactttgaggaacccaatccattt + ctgaataacggagatttagaaatgtaaaaggtagcaaatgtaaaaagtgccaggaccatc + acagcagtcaatgccaacaccaatttcccttgccatgacactgttggatcttttgaagga + gatttgtaacctggaatctcactataatgaacacattcaccggattcacacttcaaagta + atataagggtcaccaaacacggtcaatatcaaatcattcatagaaggctcactgaattta + cattgccttgtttctaaatcacagctgaaatctcctggcccttttattgtctctgtcagg + aaatccgagatatctatagaccccttagcaccacacaacacagtgtcgggaacgcatttg + cattgaacgtcattacacttataatgggaggtattctgttccaagtcgtattcaaaggca + caatcacttaagccacaatagaagctttctaactgatctatccaaaactgaaaattacat + tcttgattaggtttatcacaggcaaatgtaatttgtggtattttgccgttcaaaatctgt + agaattttctcattggtcacattacaacctgaaaatactttatctacaatcataccattc + ttataacatgtccccttaatactaggatcaggcatgaacgcatcacagacaaaatcttct + tgacaaacgtcacaattgatccctccccatccgttatcacaatgacaggtgtcattttgt + gctcttatgggacgatccttattaccgctttcatccggtgatagaccgccacagaggggc + agagagcaatcatcacctgcaaacccttctatacactcacatctaccagtgtacgaattg + cattcagaaaactgtttgcattcaaaaataggtagcatacaattaaaacatggcgggcac + gtatcattgcccttatcttgtgcagttagacgcgaatttttcgaagaagtaccttcaaag + aatggggtctcatcttgttttgcaagtaccactgagcaggataataatagaaatgataat + atactatagtagagataacgtcgatgacttcccatactgtaattgcttttagttgtgtat + ttttagtgtgcaagtttctgtaaatcgattaatttttttttctttcctctttttattaac + cttaatttttattttagattcctgacttcaactcaagacgcacagatattataacatctg + cacaataggcatttgcaagaattactcgtgagtaaggaaagagtgaggaactatcgcata + cctgcatttaaagatgccgatttgggcgcgaatcctttattttggcttcaccctcatact + attatcagggccagaaaaaggaagtgtttccctccttcttgaattgatgttaccctcata + aagcacgtggcctcttatcgagaaagaaattaccgtcgctcgtgatttgtttgcaaaaag + aacaaaactgaaaaaacccagacacgctcgacttcctgtcttcctattgattgcagcttc + caatttcgtcacacaacaaggtcctagcgacggctcacaggttttgtaacaagcaatcga + aggttctggaatggcgggaaagggtttagtaccacatgctatgatgcccactgtgatctc + cagagcaaagttcgttcgatcgtactgttactctctctctttcaaacagaattgtccgaa + tcgtgtgacaacaacagcctgttctcacacactcttttcttctaaccaagggggtggttt + agtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaattggtc + aatgcaagaaatacatatttggtcttttctaattcgtagtttttcaagttcttagatgct + ttctttttctcttttttacagatcatcaaggaagtaattatctactttttacaacaaata + taaaacaatgtctttatcttcaaagttgtctgtccaagatttggacttgaaggacaagcg + tgtcttcatcagagttgacttcaacgtcccattggacggtaagaagatcacttctaacca + aagaattgttgctgctttgccaaccatcaagtacgttttggaacaccacccaagatacgt + tgtcttggcttctcacttgggtagaccaaacggtgaaagaaacgaaaaatactctttggc + tccagttgctaaggaattgcaatcattgttgggtaaggatgtcaccttcttgaacgactg + tgtgcgtccagaagttgaagccgctgtcaaggcttctgccccaggttccgttattttgtt + ggaaaacttgcgttaccacatcgaagaagaaggttccagaaaggtcgatggtcaaaaggt + caaggcttccaaggaagatgttcaaaagttcagacacgaattgagctctttggctgatgt + ttacatcaacgatgccttcggtaccgctcacagagctcactcttctatggtcggtttcga + cttgccacaacgtgctgccggtttcttgttggaaaaggaattgaagtacttcggtaaggc + tttggagaacccaaccagaccattcttggccatcttaggtggtgccaaggttgctgacaa + gattcaattgattgacaacttgttggacaaggtcgactctatcatcattggtggtggtat + ggctttcaccttcaagaaggttttggaaaacactgaaatcggtgactccatcttcgacaa + ggctggtgctgaaatcgttccaaagttgatggaaaaggccaaggccaagggtgtcgaagt + cgtcttgccagtcgacttcatcattgctgatgctttctctgctgatgccaacaccaagac + tgtcactgacaaggaaggtattccagctggctggcaagggttggacaatggtccagaatc + tagaaagttgtttgctgctactgttgcaaaggctaagaccattgtctggaacggtccacc + aggtgttttcgaattcgaaaagttcgctgctggtactaaggctttgttagacgaagttgt + caagagctctgctgctggtaacaccgtcatcattggtggtggtgacactgccactgtcgc + taagaagtacggtgtcactgacaagatctcccatgtctctactggtggtggtgcttcttt + ggaattattggaaggtaaggaattgccaggtgttgctttcttatccgaaaagaaataaat + tgaattgaattgaaatcgatagatcaatttttttcttttctctttccccatcctttacgc + taaaataatagtttattttattttttgaatattttttatttatatacgtatatatagact + attatttatcttttaatgattattaagatttttattaaaaaaaaattcgctcctctttta + atgcctttatgcagtttttttttcccattcgatatttctatgttcgggttcagcgtattt + taagtttaataactcgaaaattctgcgttcgttaaagctttcgagaaggatattatttcg + aaataaaccgtgttgtgtaagcttgaagcctttttgcgctgccaatattcttatccatct + attgtactctttagatccagtatagtgtattcttcctgctccaagctcatcccacttgca + acaaaaaaagtctaatcttctgcaataatttccatccttggcattcagagacatatattg + gtcaatcgggtttaattttgtttcttcttcttcttcttcttcaattaacaatgtgccttc + atcatttttcatttctttcagttcttctaattctcttggagagaatttgactcccatgaa + aaatttcttgattatctcagaagaataccacctctttctttctccatacccaacaatctt + cgctgtacgaaatctctccactattctttttaagaatagcttttccaagtttggcgttag + ctgtaaaaaacaatggatatagccatccttgtacaacttaatacaaagcgtttccatgat + ttttgccaactcggtcgtgtcattacaaaacggcttgaaaaataaaagatcaatgtcacc + acacttggaatagcccctattataacttccctgtaattcgacttggcattcaggatcaat + gccacgcagcgctttttgaaccttctttaaatgtgtgaaacattcattccgagacatctt + gcataaccaatcgtcgtaatatgaccaaccaaataaaattgtccaatctgatacaaactc + ctctggatccttcttagctgcaacacaaaagctttcaaaatttagaagattccagcgttt + agcaatttccgacccaatgccgtaacaatttttgaagtattttaacttgtcttctaatcc + cactgaatcatttaaacctggtaaaactcccgtatctaatataacttgtatttttttggc + aatactaggcccgatattccttaatttagtatgtgcttcttcaccggaacgaacattgaa + atcgcaattttccatcgactgtttagccagtctataacttcttgcacgaaatttctcacc + ttcgatctcatatttttttgttaaccttttcaacgctccgataatcaattcattgttttt + gtacttcgttttgtcagtaacaagggaagctcttcctttatcctcctgtaaaagtcgctt + aagaggttttgaagcttgtatcatatccttattgttgctgtcatcattcctttcactttc + tacgtcagttgaaatctcactctctttatcagtagaactttgtccgtcatcagcgttatc + tgatataacgataatggatggttgaaatttaatcaaatcatctttttgaaacgcaaattt + atcattctggacccactttgtgatgcaactggttttcacacattgaatgcctgactgttc + aatcttaccaagaaattcatcgacatcatttaagcctgcttccctttggaaaatatcttt + ctgagtcaaatgcatatcagaatcaacaaaggagtcctcaatcaaaatgataacttcctt + acggctagattgaagacaattttgaatacttgacacaattgtggcgccctttttctccaa + tatactcttaaagaacttattggaagatgtgttaggattaggtaaaaaggcgaaaaattt + accctttagagacatgtgcgatttgtctaacaggtcgcatgtttatcctttacttattac + cactgtgacatataagttctttgcaagcccttaaaattattagccccatgtatttgaaca + tctcaaagtaattttagaccgagaccgattacggagatagaagaatagtgcaagcgaaat + actattttatctttacttaaaaaagaaaaaacaggaggaaaattagcggtattccattat + tattagcactgcaacgtcctggttatataaatttgaatatacaggatttcatccgtttct + acatttttgttacctgcatgatcaaatcttttaaagagtcccaggatttcacaaggtagg + cacctttaccgccttccttttcacaaaactgtaaccacgctggaccattatcagcttcaa + aactcgagattttatcctttgggataccaataatcttttcggtaatttttatgaatttgg + aaggattttcttgcgggtttattagcagtacaccattagtagatggatgtaatatggaca + acaagtcagtctcactatcccctatataccagtagctgcaagaatttccttctttattgc + aacctgagtcgatcttatctaatatttcacccagtatcttgaccttatcagagcctgtca + ataaccgacaatcaaattcaccattgtagctttgagaacacttgtcactaacttttttta + gatcgttacaaaaaatatgactatttttaagtcttctgtcaccaatgacttcatgaataa + actcttttgaccaatttatagataaaacgtaaaaatcactttcaaaatttttgacaactg + aagagcaaaacgttttgaaaccatctcttagtaaacaatcatcgtgattctgatctctgg + cgaacgttttcatttgatccaaagaaatcgacttaaaaatctgttgttttgtaatttcat + ttacactatttaactcaacaaccctgttatgattttgatattttaattcgtccgcaaaca + acttattgaagttagactgcgaaattatcgtaggtacgcctgaagataacaacggcagtg + atctcgttccattgtatttgtatttatggtatccgtccatatatgtcttggtaaaatgac + cccactcaggtttcaaccgcgggttcagtagatacggtaatttagcaatggtgcagattg + tgtcaactcttgtgattgtttcatcaaaatctgaaataataatggttttcatatcctcca + ctgttcgcttctggttactgcaattgttaaatgcattggctcgattgtcaacattgcttt + gtgcgaagaaaggtcgcttttaaagaaaacgataagaactaacctctaactacataataa + taagaaaaaagtatacagataaacaagctcagtagtaatacataaaataaaaagttttgc + aaatcgattgtccacacacttctatatcaattccaactaagaaaacgaaggaaccgactc + aaaccggtgtaaaagctaggctttcaatctctgctcctccaaacaaaactagtgcttgat + atcacatccaattaaattacctaaataattttagagtgacaccatcgtacaaagagggca + ttaagcttattttttttaattcttatgaaaaaaaaatgtggaagagagcagaaaattttc + ataatgaatctcttctattcttatgtaccgttccgccaaatttttcatgaaaaaatttgg + gaaaaagaaaaaaaaaaaaaaaactagtatataaagtaaaaaaagatgcgcaagcccgga + atcgaaccgggggcccaacgatggcaacgttggattttaccactaaaccacttgcgctag + taatattgatgcattattcaagaaaagcaaaaaacatattagccgataatttctatttta + taagtaaaaacattggtgagatagtggtaaagagttagataattattgggattccattgt + tgagaaaagcaataatattatgtatacggaatatactaggagttctcttcaaggatagag + gaatcctcaaaatggaatccatatttctacatactaatattacgattatcccttcttcta + ttttatatgtttcattatcctattacattatcaatccttgcacttcagcttcctctaact + tcgatgacagcttctcataacttatgtcatcatcttaacaccgtatatgataatatattg + ataatataactattagttgatagacgatagtggatttctattccaacaagaacatccttg + aaaggtcgactattaacataagaataagagagagtgtgtggccgatgctgttgctatagc + tcggtcgtagtttaacaccacggcattattcagctttaaaacaattgaattataaacctg + cttttcttactacgaaaagtgtttccctttaataaatctattgtacatactttacagttg + atagtaccttcaccataaaaatatgcaaacgcttttgttattgagaacaggaataccaac + cgttctcatggaaaatagcaaagtgtaaaagaatttattatcatcgagtgataaagaata + tcgccagatctttacggatatatatcgctaaaaatatttgtcgccaataaaagatatgtc + taacgctccctttgcaggtagttttttactggcatcgcctttttactgcaaattattgaa + aattttcgagcgagaaaaaggaacgaaaagagatgagctaaattcaaatatcaaagtact + gtggtaagtgctttctttatcgacaaattacaagcagaagaaacattcactttacaatta + aatgtccgaaaatcacgttcctgcctggaaaagaattgctttgaagagacagactataag + cagtggtgacgaaagtaaagaaaaaggccaatccaatctaatagatgatgatccactaaa + tatcactacgcatttatccactggtaatttgacaaagaaagagaaaaaaagaatcattaa + tggtgagagcaaatcctctaccaaaaaaggaaagcgtgtgtcgaaaccagggacgaagaa + gaaggaaaaattgtcaaaggatgaaaagaattcgaaaaaaaataaaattcttaaagatca + attacgttacctgatagaatttttcaggacaaagtctgagagcaagtttcccaccggaat + cctagaattggaaagtgtaaaagaaaattacggcgactccctgatcaaagacgagccatc + agagtctggtgttgttgaagtttggaaattttccaagcaaaagcaaaattggcttattaa + gcattttttcaatttggacgaaatcccctcagtatataatgatcttttgcttttatattt + cagggacttacaaggtaaatcaaaagaagaactaatatcaaaatgcaaaggaaaactgaa + gcaatggaacgactatgtggaagatcaagaaacaagaataaaagcgttaattgcagagga + taaggctagcgaaccaattaatggtgaagaaaaagaagaaggtgaaaaagacggtaatgc + cgaacaagggaagcaaaaagaggtacaagacgagcaagaggaagtacaaatgccgaataa + agagcttgttcaaagaagtttgaaattactagaaatttggaagaatgatgactcggagca + gatagaacttaagaatttcttcgttgatgtttaacatagagttcaacaacgttgttcagt + tgtagtatatattttgctgtgaaatacgctcaaaaagtagcgggataaatcgtttcagga + agtataatttcttacaaaactataacaagatcgtctaagtaacatacataaagaaatcac + ataaatttttaaaattcgaggtcaaaaaaatcaggtggatacataacttaccttaagtta + tcgagattttcagtacacacaatgtgattacactgatttataaaaccaccttacggcctc + ttataagtaataacgtggctcatcaaaaacatggtagaagtgacccctctcaccttcgcc + taaaaacttatctggaaatttcatgtcctcatattccggttcactcatatgaccgtattc + atacatagaatccgagtgctctactaacgcttgctcactcaaaacttggaacttaccaac + ttgtagctccgtatcggttatcgttcctcttgcaactctagcatatcctgttcttctcaa + gcccttatacaagatatactcacaccatctatcatcgtcagagggatcaatgtcgtgcat + tccggatgtttcactaacgtacgtattgtagttgccttcacctggatcaacaactaagta + acttaataaaatagctgggcgagtcgtattgcccatgagcttagccatgtagttactttg + cagtcttctatcctcttcatcctcttcttgtccactatggaatacaaagacgtcaacgag + agtgtcattgtacgtttgaagtgtggcatgaatggctggcgcaagttccccaactggaga + gggcaataaatgatgcgtagaatttacgatagggaatttagaaagaagaacacagcccca + ggtatgtttatttggacctggtccgaaatctgcatacatattcaaatcatgagctagttt + gctagttagatccctgttccccatggtaattctttgtgtatctgtttctagtagacctac + cacatctagttccatatctttaataaggttgatcattctgtcttcagatgcccacatatc + attatcgagaccaaagtggatagtccaaataccagctgtaatcaactgagaatctggatg + ataaggctgaggaattcctgtaggtctaatatcatacacgaacctagctgttagtgatag + tagggccacggcaaagaagaaaacataaatgaagaattttttgcccatagtcaccagttg + aacgttaaggtttttgcatgttaaagcaccaataatgataaatgtggaagaaaaggcaag + aaccgtctcaatcttctccctcagtacccagcccattggaacaaatgcgtatgcaacgac + ccaaacatgggcaaggacaaagataatataaacggagaatgataggacataaacccatat + gttttgaacttggcctaatgcagaaaaatacgaaggaactaaccaaagcatagcgatagc + gtacaataaaccaccaaaaatatatttaggccattgtgtaatgcttctagctgaaagtac + agcagtggatatgagaagcaacaaacatggaactaatggcttgcccataaacttcacaga + actcaaagaagcaaataacatgaccgtacaagttaaggcgccccaaggccatggcaaggg + accgtgtgattcattgtaaccttcccatgcccaataaatagtagtagaagaatccgtcaa + taactgatgaattccgaataacaacgagccaaaaccaacagccaaaaataatttgccgat + caacgaagcagattttgcttgagcattccttttcccttcgaaattaattgaattaacata + aggcgacagcatgccgaataaaacagtaagcacgagagctgtcttattatacccaccatt + acgttcatctaaaatgacccaagttgggttgttggtatagaaccccattttcaagatcac + agatgcaacaagacctagcagccaagttagagcaaaagagaaactagtctccgcatttgt + gatatatcttagattttgtacaaacgttgcaacggtaatggaagttcccacagctacaga + aatcaaccttaattctggcatctgaacaatataggcaccaatggcaataatacctcctaa + aagtacaccatattgcatgaaggcttcagaaacgaacggcagatataagaaaatgggtcc + caaataacccaatattgcagcttcataacctgagattcccatataccatagcgggaaatg + ccaaatactacataaaagggacgtaacgttcgaccagaaaagaaaaccgttaaaaatatt + tgttagtaagtaaaaaaaagagtcaaacctgaaatatgaaccagttgcgcttctagcaac + gggtttttccttttcaaccttcttttcaccacttttctgcaattctgaacttttttcttc + tccataatttatgggattagagtctctaatttggaaaaaactggtattaccgggtttctc + attaaaagcgagaacaatatctatctttttgaaatcagcgtaagcaaatgcatcaaatgc + aatatctaaaagaatcagagaccattcgaaataagcatatatagaatatgccccagctct + ctgttggacggagtgctgaatgtaccagtaaatcatcgggaacaatgttccaaaaaaaat + tgtagcagtcaaccctttgttcttcgaagttaaaggactagaatagcgggtaatcattat + atcccatggtaacgttaaaacaatgtatgtgatcataaatatatcatgaatatcgtggtc + atctgtacttgtaatgtatacccaaccaccgcaagagactgtccggagtacaccgacaag + gaagcataccttagattggttcaagtagtagtgacctagtagcagtaaaaatcttggaaa + agcagttagagctattaagatttggaaaatagaacgttccggatagcggtccccgatagt + ggctgatacactaggaaaccactcatctggatacgtataatgtgcattggttacaatttt + atgaaaatgtaatgaataaccagtgaccaaagctgcaaagaaggcggagaatgcgcaaat + agtatgagctatagggatgatcttcccattgatgatcagcataactggcggtggatacct + tctgtattcctggttacttattcctcgagaaatcggacattaaaagggtgcaaatactgt + ggagaaattataagttaccaagtcaaaaagcgaatgagaatctattgttgcgattttttt + ggttactacttcttacccgaactaactttacataataaaaaagcatgaagcgcatattca + gggactcaaaaatcccaaactggcacttctgtatataccgatgagcatattttgattgta + aaaaacaacattaaaaagaactgcaaacctgaaccaagaatacttaatttcaaaaccgag + aaatgaggtacaacttgtagaagttcacttaaacttattgagcatacaatctcaacaaaa + ctaactgcaaagtttgttgctatagtcacatagtaatactagcaggaagccattaatcaa + aaagaaactccatataaaacgtaataaatattatgaataatagtacaatataagaacaaa + gaagaagaaaatacattataactgaagtgccaatattagaaaaaaaaaaggtggaatatc + ctagtggaaaaatatcatctatgcggtgaaagaattaaatagatactatggattgtttcg + aatctatccttttatctgccgcttgcctttttttttcattctccttcttcagtctttttc + cataggcgagtccacagggactacaaagttccctgttttggtcgggtccactacgccatt + gaattgtccaagtgtctttacatttagagcattcctttatctcaccatttgggaggattg + tttgtctcttttctcttttaggacgactagtagtctttttgctgtatttcttcgtaatac + tggcagaccgactagttccttgtgcacaactatggtagccttcaagattgtttgcttctt + ggctagtcgtacgtctctcggaattgcatgcactaataacacgattctttgagggtgtaa + tttcgttcaaagagctggttttgttcttaacaatatttcttgtaagatctcttagttgtt + cggcattctcaatttttttttcaacatcaacagcccttatttgagaaaaggatacttcaa + taaatgagcaacttgcataaactcttccgcgttccttatatgccttctttttatcgtttg + acttccagtcatcaatttctctatttaataataacgttttctccatatctgactttttaa + ctactctagttaagaaggaacttccagagttgtcataattattatatcgcatactagcaa + tagtaaatgtctggttatttacctagcaacctcttaacaaagcgataacaatgaaacagc + aatataagtctatttaattgacaacatcattgataaaagtttgaacaagcgtctaaaagt + cgaagaagttcatttcaaagttttgtgtatcgagcttgatgatacttatttagggatccg + ctctagtttactgtgatacaactgaaaaacattacaaagctatcgcgcctgcattctcat + attagtggcgtatgtttgtgtcacattcgatacttctacataatacaatgactgaaccct + taccgctggcacaggaataccgcgccgcgaaagcagggtctaggagaaacaatagaattc + ttggcgatgagagaggggaaggctttaataaaaaaaaatagcgcttatccgattctgttg + gaataagtggctatcaatatttacatgttgatatggtagtaaggatataaatgcttatat + tgaatatttggaataagcatcaactatcacttattgaaaaggactcgaacaaaaaaatat + gaaatgtcgaaagccacatcactgagaagttgttggattgaagctctaaaatgtcatctt + ttagtagtattcgtgttactagtatattatcacatgcggtgtaaaaagatgacgtaaaga + ttgagaaacagtcatcaactgttatggaagctgaaatgcaaatattgataatgtaatagg + ataatgaaacgtataaaatgaaaaagaataataaaatattattatgtagaaataccgatt + ccattttgagaattcctatatccttgagaagaacttctagtatattctgtatacataata + ttactgcctttatcaacaatggaatgccaaaaattatcaaaatatcacccaatattcaat + tttattgttgtgaagacatcaagccctagatatatatcatagtaggataaaatagtagaa + taaaactattcatatatctacgtgaaagcatcagagattgtaatgttgagcgccgtggcg + cagtggaagcgcgcagggctcataaccctgatgtcctcggatcgaaaccgagcggcgcta + ttaaattttttgaactgtccattaataataatggaactgacgtcgtcaattaccggtagc + ttttttgcccacatttttaatcatcttttaagtatttgagtatttgttgttcactagagt + tttactaattaaggttctgagaaaataaagatatgcatttagaaaagtcggttctacacc + gcaaatttctttattacagaaacattttatacaaagaggatacatagttattggaaagaa + aatcaattatctttttgttgggtagtatgaatattacttgtacattatcatatacaatat + aaaaagatgtcatagagttgtgaaatatttattatatttaataaaagctgaagcgcaatg + attgaaaacgttttaggatgattaatgataaagtacaaaatgaggagaagaaataataat + aacataaagtggaaataccggttatcttttaaggatattataacctggaggaaaacttcc + tgggttctctgtatattcaacacttttccgttatcaaaaatagaatcccaacagtatccc + cattttcgtcaatatctccataacgacaaggtcttctagataccatataatgacaataac + caagcccaccatttaggtggattttttgtgtggttttgaaattaaaacatttttagagga + aaattgtgatcacagaatttcacattcccagttactttaataatattcatagttagagat + gaagaggtactattaatatgaaggttaagtatgaatcttctcatgcttctataagcgagg + gatataaaacatcgccaactgtttcatcttatttattatcaatattatgtgtgtttggag + cttactatacagttaatgtgataattaatataactcttgtataactaccatatcaatgtt + caattaatagatcttaaatcggtttcattactacctgaactgcatgtatgaaatttcctt + ccacattaggcacattcacatgtaatacgagatgtacatctatatgtgatattagcattt + acacagcaatcctatatcactattaatggccacttgttccaaaaagtatatattatgaag + cgtattattattttatactcagatagctggtgcgatacgttcgcagattttgagcgcaat + gcaatgagtatgacggaaaggttcactcataatttcaataactcaagttgtttgaattta + aaatacattccccgttagataagctataaacattgttacttgcgcttgcggcacatgtaa + tgtagtttctttctttttccaagaaaaaagaaagccctgtagggggctcgaacccctaac + cttatgattaagagtcatacgcgctaccgattgcgccaacaaggctctttaattatgaaa + aattacgtttaataagtgacttaaaaatataaatactgttactaaaatgtgaactaaaac + ctcaatattattttctgatcacttgatgaatagctggtaattgttggattccattgttgg + taaaggctataattttacgtatatagaattgttggaataaaaatccactatcgtctatca + actatagttatattatcaatatattatcatatacggtgttaagatgatgacataagttat + gagaagctgtcatcgaagttagaggaagctgaagtgcaaggattgataatgtaataggat + aatgaaacatataaaacggaatgaggaataatcgtaatattagtatgtagaaatatagat + tccattttgaggattcctatatcctcgaggagaacttctagtatattctgtatacataat + attactgcctttatcaataatggaatcccaacaactatctgaaaattcacgtgattctca + tgtgagtacatcagaaaacaaccacctaacaaccgtgtttgaatgttgatctgaaaacaa + aaattaatctctcgtacttattatggatattgtattttctcaaaattccaaatgattttc + ttgttagatggtattcacggtcatcggaatctcagttcaatatcaccaatttgcttataa + ggtttatgcaagataacatgtttggtcttgttcccaagtataacttaagaaaaaaaatta + caatccaagaaaggtaatacagaattcatagcaataatggggtccattttcaatagataa + tgtacctggaagaaaagggctgactaggaaacaaaaagaaaaacaaaactatgatactcc + atcaaacatagggaaatttactcgaatatgaaaagtacaaatctgtcaaaagcacaccgt + atattgtcttggttccgcttttattcagaagaattactgaaatgaagaggaatgacgtaa + taaacaaacaacgattctgatatcagaaagacatcttgaatattcctatgtagtaattaa + ctatcccagtacttacaaacataacttgacgatgcacgctacgatttttgtgtgtttaaa + cttttgttggttggaactcaagaagatcttctctgagataattgtaattaaatgtacttt + aggagtgttcatgtataatttgcctctgcaaaagcttaataatgtattttaaaacttgca + tgttgctctgtgaatctgaatgtatgataatgctacgattactctagggaacttcctttt + tacatttaaagtttccgagatcctcagctgacaaataaatgtcatatcaagacaataaac + ggtagtgctaaagtaattgcatattaaacaggcgcgactaaagctttcatcccttctatt + attgcataacctggctatacatgaaaacacttctcacatttgatttaagaagtttactgt + tctaagaaaataaagaacgtgataatgatgaatgaagaggattctacagaaacgaaaagc + ctagtcataactaatggcatgtttatcatagacgacatcgagcgtagtaaatataatatt + cactataagaatgtcccaggaggcggagggacttttgccattttgggtgcatgcataata + tcttccggcaatgtcacatccaaaggtttgaagtggatagtggacagaggctctgacttt + ccaaaggaagttataagggaaatagactcatggggtactgatgtgaggtttcgagatgac + tttagcagattaactaccaaagggttgaattattacgagggaagtgatgatttgagaaag + ttcaagtttttgacgccgaagaagcagattaacgtcgatgactggatttccacatttggg + cagaagataattgatgaaatgcatgcgtttcatttgctatgttctgggtctagatgctta + gacataataaacgatctgctacgggtgaaaagttcaaagggcacaaaaccaatcgtgatt + tgggagccattcccagatctttgcgactttgatcatcaaaatgacattaaaagtgtaatg + cagaggaacgatgttacggtaatattatctccaaatgccgaagaatcaagtcgcttattt + ggtttaagtagcaaggaaccgactagtttggaagaatgtctagcattagcgcatcgtttc + gatgatttcatggatgaaaacaatatgtgtattctacgatgcggtgccctcggaagcata + tcggtaagtgagaagtttaagaacggacgaacctatgaccatttccccgcctaccatttc + aaaactcagtctaaagtactagatcctactggcgggggaaactcgttccttggcggcttt + gcagtttcttatgccctaacgaaaagcttagatattgctagtatatgtgggaacatcgct + gcaggcgcaataattgaacaattcggaataccgaggtacgatccaattgctaaaacctgg + aacggaatcacattcttggatagactgaaattttacctttcacagtccggtcttcaatat + aatataaacgatctttacaaaagtctaacacgatgatcatatcagcttgttcattgctct + tatttttctgcaaaaatatacatatatatatataagtaagtgttatatactacttgagat + taggcgttgtaacaagattcaaaaaattcgaactcgaactgtgaaaccttcacaaatatg + ctttccacttcttcgacgggaaacttgtcaacttcagcctttagaaattcacaccaagtt + tgaaaatgctcaccatcatgtaaatcaatccatttttgatatttccaatgcaaccctggg + gctctaggagtatcacgagcccatctccaatagagttcctcagcaacccataagctggtt + actaaggatggccatgtaattgaggcgtcctttctcagctcatctaagaaattaatatac + gcatcaacgccggggatcgctttattgtcaaaattatctctttcttccttggtgagggat + ggtgccaataattctaagcagtcatgaaagtatgagttttcatcattagaaaaaaatcca + atctttttggctaaggttattaaagcgtgagtagttggtgctaaagaagtcgtcttacat + atcaaccttaagctagtttcaaaaaattgcagatcttgtgataaatagatgtacaaacta + cggtccttcaatgttccggcacatagttccttagcgaatttatgttctgtggttttccta + acaagggcgtcgtacttttgtattaacttatcagtggtacagctcattgttcttccttgc + agatattgttgatattgttattatttgcgctaaattttagttaatacagaaaaaaatatc + taagttcagtccagttcatattcgtttatatatctttcgaagccagaccttttttgccaa + gcaattgttactatggaaactattagaaccatatgcgctaaacaatattagccatcaatt + caactttattcgcagtaagctcctgcagaacaggcttatcaatcattatggtcttaacgg + aacgcctagggacgctgactaggcccatcattctactactggagcccatacgttcctcaa + catgatctaatagcaaattcatttgggcatctacagcaaccaagcttccaactaaaatac + gatcttctgtaagggaaactattaaagtatttccaataaaatctgacagtttcaagatgt + ccatgacgctttggtgccccttactaagtttgttgaagactatcttcaccctatcaatga + tatcatcgcatttaaaaaatagtcagaactcatgcacttcgcgagatattttttgaaaaa + aaaaaaattaatccgccacataagatgcacaaaatgagcctataacattgaattttatga + tagaacgaaaggattgtgttatttagaacagagtatcagtatatcgtatgtcacagaaca + acacaatcagctcaatgaatcctgaaagagcttacaacaatgtaacgctgaaaaatttaa + cagcatttcagttattatctcaaagagaaaacatatgcgagttattgaacttggtagaaa + gtacggaaagacacaatagtattatcaatcctgaaaggcaaaggatgagtttggaagaaa + tgaaaaaaatgctcgatgctttgaaaaatgaaaggaaaaagtaggaaaacgcaacattca + caattgtaagctacaaaatattgaatgtgaatcctgcttcattacaaatgaagaatttca + aagcgtatctagttcaccaaaagtcacaatatcttattattatttggaagcttttatatg + catctaatggtcataaatcccatctcactttataaaacaagttaccgcgaatgtatccag + tgatacagaatagagtgtaacaaatgagaataaaaataccatattatcgtttaattttgg + tagggtacgctaaaataaagtggtgtttgcgtctccatataactaaaaagaattgaaatg + gcctactatcgattagactattcaggcatggaagcagtaagaacatttcttgattttgga + aaaatgagattagattattaattattattgagtttgtgtatataatttattttgcatatt + tcataaagaaattaaaattagattattaaatattaagtttcattagtgacattaaaagaa + gaaaactgatgttttgaaatgtgttaagcaaagaatgattaagacaatctcaagctgctc + taagcagtatcttcgacagcttgctctgcttcaggttcgggttcgtggattgcagtctct + ccagatgctcttgggctctctggaacagcttcttttgtttcaggagccgcatcttccaca + tcgtccgtagcatggtgatgagagaatcctcctgtcaaagatagccttggcaatttgcca + tcacggctgactgcaattagcaagtaacatggataaatggcaaatacacataaatccaaa + acaccataaaagatagcctcaccgtctggttgaatacggttaccaccatcacttagaccc + caacagataaagtacaaccaaacgattaccatgcaggtacacagcataagtgcattgaac + cctctagttttcaaattgaagaattgacgttggcatatcacaccttgggtaaccaacata + gcgacagcaccaatggtgtaataaccccacttgtaggtggacttgatcaatgaaccaact + aatagcgaaacaacccagaataaggtacccacgatttgaattaacagcgaatgtaccata + tccaaggcggaaatgtcgtcattctcaccagtagtactagcggctaactcgattaaaaat + aaaaggcatggccaggacaagaaccaggcaatatatttcgagtaaaagatttgtctaata + ccgggaacttcacctgtgatagacttgctaaccttgacgtggttaaattcagcttgaaca + ccagtccaacctaaatcagaagcataagtgaagaaagcaaaaaattcaaagaaagtgatc + aaaaatgcaggagctaaggcatatctagtcaatctggagcccttgttctccgcaatgaag + aacatcacaacatagcatagcaatataaagccaaagactgcaaacactgcccataaccaa + tccgaacctctcttggtaatgtgcatatccaggccatgtggtggattaagccctaaagcc + tcgttacgatttaaaaagcttgatagcgtatcgttcatttgaaatttgttgtttttagta + atcaaaagatattaaagtctcaaacttgttgttttgtttttgtaattataaataataata + attgagttaatatttgttgttgtgttgtattgcagaatgtaaaaacaagaaacaagatta + gaaagtgaataggtgttacgctaaataattgaagactctcacagacttatatatgatttt + tttagtgtgatacaacttgtaatgaggtaagggattgatattatactttctaactgatga + tgaatatgaaaactttggccaattagggagaggaaagttttcgatgagatgctagaaaaa + aacttgtccttgccatagcagaaagggatgatgaaataacgaagatgagctaatttggta + ttcgaataaacatgcaggatgtggcggaagggatcggaataagagtcgggatcgggatcg + ggatatggctttgcttactttagccataccaaatattcttacaaagcacgctttcgatgc + gtttttgccttctgagagaatcctcctacggctcgacccttgaaaaattctagaggatct + ggaaaatggccaacaaaatcgcgcagggagacgcaaggaaagtaagtcagatggatccga + ctgtatgtaacaagggttgctgcagcggttttccatccctttcggcaccctgcacaagta + gttattcttacttatgcaaaacggatatgagctacatagcgttcattttttaggttttca + gaagctattacaacgcagtaaaaaggatacacaactttcttacggggaagaagaatacgg + ctcaatgcacccacttcaacccccggtatcacgcggcactaaagttgtttgattgtaata + gtaatactgctgttgaggttttcttcttcagggctgctcacaacgtgatatatttgaatt + gacaggacgtattcagcgcagttgaggaaaattaacatatgacatgcgcaggaaataagt + gctcaagttttctacttttgaagaaaggactttggtttacttgcctactatctccatgtc + aagtactcggttgtatgatgttaaggagcgcagggaacactaatcgataaaaaaccctta + ttggctattactaaatgtaacggccgtgctctagtgaaatttgtatagaaaaatgggttc + tctcgctcaaaatgggcgccattacttgtctggtcaaattgagttcctggatataattac + ataaacggattgaaatttacataaggagtataataattccggaaactatcttgagtgcaa + tgccatcacgcggccatgccttgacataacatgctctcatcaaaaaggtgcaaattttcg + ccagttatggttgatatctaaaatctactggagttcatttttttttttattagatatcct + ttttgcaagccttctgaaaaaaaatttctcatgtggtacagcgttggttacttaagctgc + ttaaactagatcgatgagccattcttaaatcagaagactgtgactgcgaattgtaggcat + cttcactaccctgtccatgaagctcattttcgttatcatcaattggtgctatcttataac + tttgataaagagctaccatacaaaaaagacttaacgaccaccaactgacccaggcgacgt + cattttgctgggaccaagacataatatagccccatactaagggacctatgaaacgagcag + aggcagaaatactaatggtggcgccattgatgactgatctacaactcaagggactcgaat + tatgaattaataacataatttgtggtgacgttaaagcgccacaaaatgtttttatccctg + tgattatgtacaagtagacgacagtataccagctaggaatccgttcattctgtagaaaaa + caacgtaaggaaccataacgtacataatagggtacagcttgactaaagttctgaaaattg + ttaaacaatcgaaatttcgatcaactatgggaaaaatgaaaataaccacaaaacaaccaa + agatacctgttgtcgacaaaagagtaccggtttgttctggttcataacctataccgccag + atattttccacggaaattttgaagccagcttctttggattttctggatctacggctaaat + cataagctaaaaaaacaggcaaaaattcgttgtatacaatcaaatgtaaagccataataa + aattcaccgatatagggtaaaatacttttgtatgaaaaacgtgatgaaatatactacttt + ctttacaaccgtctggagctgtatctatattggcatgcacagcgtctgttggttcatgca + gagaatatgtcctaatcaggcctacagactgtcttcttgttaaaataggatcaatcgatt + gtatgctttcagtatcatcgtcattggtagtattcactaggggactattttcatcctctt + cactatcttgtcctcgattgctgttcacattctcattacggtggtgaatgttttccgaat + catcctgaatgcgcttttgccagggtcttcttttcggctgtataccaaatatattcttct + taataaaatcaccgacctctaaaccgtaatctcttctatttttaaaagcaggatgtgttt + cttccaaaaacaatgttgcattagttaaaccaaacatcaaaaacatgcacactaccacgt + ttggcaaggcgtacggatatgacctaattattctttttgcaaaatgtggaaaccatagtg + gcacttcattcattgttccatctctaaatacaagaaacccaccgatcataggcccaacaa + cggcaccaaattgaaataataaaggcatagtactgaaagctaaagcctgatgttttcttt + cagttgctatttcaccaataatggttctaataacgccgacgttaccatttagcaatccca + ttaaacttcttgccaccaaagcctgatagaaattgtgtgaaaatcccagtattaacaatg + atacagatgttcctataagcccgcaagtcaatgttatttttctgccatgtttttcagaga + atctaccccagtggtacgcagatatgacttggcataacgcaaatgatgaagataaataac + ctgaatatttggacacttgagcatcattgggagcaatattaaaatctctaaccatgaaat + aaacataaggaaatagtgacgaaaacgcaattggttcgctgaacctaactaaggacacaa + caacaagttggacccaggggaaaccatccatttgttctttgaaagtaagcttttgacgcg + ccatatcttttatattcgtgtgttcatatgcttaccacttcctagttccttggctagtat + tcctttaggtgatctaaattcaagctctgtggataaggaccattatagtattttttacta + atgatagatactttttgttatattttgttttggcattttcgtatccgagatttccaatga + gcggaatgtgtcattctttgtcaattaacatagatcaggccgaatgccaaatggagttta + gtaccttcatattgaagaaaaatgctacattattatatttacaaggatggtcttaaataa + agcttacttttaaggggggaagaatggtgaacagtgagtaaatacatagaaggagataag + aaacaaatggaatatcaaaaatcgatggattctgcagatgttctataaaagggtatggca + tcctttatattatggttgccatataagtatgagataaatctctcaaaccctagaccaaag + cctccgtgtggtgcacttccttcttttctcagagaaacataccagtccaattctccagat + ctattcatcccgcgtgctttcatttctctacataacttgtcatagtcatcttcccttaaa + ctcccaccaattatttcacccattccaggaaccagtagatcaaagcatccaacagtatcg + tcaggagtggaattttgtttcatgtagaatggtttacaaagacgtggatagtcggtaacg + aaaactggggacttaaaatactctccggctaaaaatttttcatgttcagtttgcaaaggc + tgtccccatttaggttcatacttaaagtgtgaaacttcattgtggcgtttcttgagaatt + tctattgcattggtatacgttatattgtgccatttttcatttattaaatcttcccatcgt + gttttaatttgttgtgtctcttgatttattgatagctctgacgatgcattattttcttgt + gaagagataaattgcttcggcaagagttcttgttggttatctatacaagctttaattatg + tgttttattgtagtctcaacaaacgatgttagctcgttgacgctattaacaaagcacatt + tccacttcaagcatccaaaactccgaaaggtgtcttggagtatcactcttttcggctcta + aagcaaggagataacgtccaacaccttgacagtgataacgctaaaatttccaagtgcaat + tgagtggacacagtcaaataagtcggcttcccaaagtacgaggatgcagttggcgacgta + ttggtggagacttgaaacaactcgccggcaccttcacaatcgtttgaagttaatattggt + ggtgaaactttggtgaagtggtttttctggaaatatagcatgaactggaattctacaaat + gatcttaaccgtaaaattgcacttaagtaagcggttctgtattttagtgtaggtaaggac + cttaaataacgtaaggtttgatatttcttttgtaatggatagttttctgaaacgggaccc + acaagtttaattgacttgacaggatttttgatttgcaattcaaaaggttgttttctatta + ggggtgctttgccaggtagcattagatatagataaagtttgaccagttttcagaattttt + aggaactgtacttcatcagtatttgtgagtggaataactattcttaatgggttcacagaa + gtcccatcttgtaaatccaaaaacgctatacgttttaatagtcttatggatttgatccat + ccattaattgaaatgggatcatggctagtatggtgtacctgctcgtacaatgatttaact + gttagtgaagagtaaaatctaccacctttaaggaaggtgaaagcatgaaacattagctgt + ttgtattcgcttgttctctttctttctctcttagtaagcgataagtcgaactctacttaa + attgtggtacgttttctttttttcctataatataaaaaagataaaaaatcaggacgagaa + aaagtagatcataactataagttgtgcatactcatactccacactccacggcctaatctc + tgtaaggattgtattttattttttatttatttattatttagattttctaaaaataaaaaa + aaatcatacactattttgattacactactatgtaatttatctttgaaaacaaccatgaaa + aactcaattttacacacacatgagttatcatgaggagtcatcagccagacttaacaatca + cacacattgttaagagtggaaaaaaataatagtgataaaaggaaaaaagctaacaatttg + gattttacgggatctgtctaaatacgaaacacacactcgttttagttcaaatatcataac + cataaaaaaaaaagcacaattcaacttgagcacgcacactaagtagtaacaacgttcatt + tacagtaaagcgaacggacgaaacaaataaaagaaaggcatagtgagggataaaaggtag + aaagaattcaaaaaagggtatcgcacacacacacatagtagtctaattcatgttctagaa + tggatataaacaaagatgaaggggacaggaaataaaatggctcgctccgccctttttaga + atctttgcaatcctctttgtctagcttgccattttctgtagataatggtagcaataatgg + caatacaagccaaaccgaccaaaatgaaaactaaaataacaccacctggtaaagtcattt + atgatagattaaaagtgctgaaatgggaagaggagaaacaaacaaaggatatgtaggaac + aaaagtttgcttgagtgactagtttgaagttgagaaaaaaaaagagatgaaaaagttaac + ttatttgctcttccttcaaagtgtgtagcgtcttcctttaaatactattgaaagggaaaa + agaataaatggaaaaagggtagtaatgaaaaactctaaaggaagataagacgcaataaga + gaaaaaaagagaagttaagattacaagagaaacaataaattgctttcaaagctttatata + ctggcaagcacggaaggctaaatttgaaaaaaaaaaatgggaaattattatcggaaaaaa + ttgaagctatcttctattacgcagtgacaagtctatattgacatctttctgctttctgct + ttctgtttgttgttttttttctcatagttgttttgattttactctgttggaaatgcgaga + attgttttgattttacccgattgcgacctattcccggctttcccaaaaaaggaattcctg + tccaatgcaacgacagccatagaaacagccgcggttgggtgcgtgggtgcggtaatttaa + cgatcatatggtatgggtgcacgtgggatgctaggggaagtgcgcgcggataccgaatcg + gccatcgaaccggccacccggattgaggttggtatgttgcctctttgcgcaattttattg + aatgcaaagtttctccttctagtaaaagacaacagctgaaaaatgctcgatgtccaaatg + acgacataaacctcctcgtttaggaaagagttaacacgcctagtttcagcttgtttcttt + ttattgtgtaattagttttcttacttagtacatcttattgaccccactgtcatcggatgt + gagagagtggttcacttctgaattaagtttagagatatactaaatctaaataaaagtctc + tttctgcgtgcatatgttttcttgcgcgggactcacaaggatgcgtcttttatttcataa + cgcagttgtcccaccttaagcttaggtatcagaatcgactaaatcgtcgatgctgtcaat + tatgtcgtcaatgagatcttttgcgtcgttgattatgtcttgaagccaatcccccactgt + gctagcagtagcgctagcttgtatgctgctgctgctgcttcttcttgatgatggtgatgt + ttcgcccggtagagtctcagttgctgatggtattgaagttgtcatttctagttttatcgt + taatgacgaagatggcatagcggctatttcatcagtgtttccatcgtcgttgtcatcatt + atcattgtcaacattgtcttgttctgtctcttcggggtacccgccccaaattatattata + agtgctgaattcaccaaacttactgaccaaataatcaaagtcatcttcaatctctacttc + ttcgctgctctggggttcgcggagttggttcataagcatcccagtcccatcattggaatt + tctgtccttttctgccacaccgcaaatatcgcacaaaaggttgtaaatttcggtattttg + gaacggctcaatgtatccctgtggaaaatatggccccataccaataaatacagatctcat + atcgatcgcgttattgtcgtatccatgtgatccaatcgtgaacacattgtcttcgttttt + gtccttatgatcaccttttgccaccttcttcaattgttctttcttcatcactgcataccc + aggctcgggcacaatccagattgacgccatgtgatgattttttccatcattaaagttcca + ttctttggggaaatttccattaacgtaaaccgtatacttgtcttcatctatagaagtctt + taaattgtggtaaacctcattgatgtttccggaatctttcaacgatatagccatcatcgg + accctctagatatgcgtgcgatacataatccttcctcaatttttcgtccagtaagtcttc + ccatataataacatttgagggaactacgatatcgctcataccatgatcgctaacaatgac + caaattagtaaagctggttaaatttctttcttgcagcgattccactagttgcttcagaaa + tgtatctacttcccccagtgtttcagtgaagtcttcataatagtattccgactctgacgg + atatccatgcttatgtccaaaggcatctacgttcggtacataaccgagaattaactgtgg + tctttcgttcagtgtactcatatccacatattcaataatcttggataatttttgcgaaag + tggttctttagcattgaattcgtcgaagtaaaatggagttctctctctagcaataggctt + tttatgttcaggttgtagtttctcttcagtcttatacttggtataattcacatcgctacc + tggccacatatgggtagcagctttgaatggtatatcaccgtcaaatgcagactgaacagt + ttgccatattggttctgtatcattattgttccatattcgagggtccaatacacctggatg + gaattcttcattaagatcaggatcccaaaatacgttagagactataccgtggtgtattgg + gtattgtccagtaaccaacgtccaatggttgggaaaggtctccgtagggaagctgggtat + cataaaaggtgtggacgtgatattcatacctccatcatatttcaattcatataagtcatg + taaaaacggtgtgttcctcttagatatgagtgaaggatggaaaccgtctagtgaaattac + tatcgttaaggggaaaaattcatgcgtaccgttaaaatacttcacgtaagggtcaaattc + aggactgccaaagtgcgatagcactctcgtggagtgatggggttttgctatgttaatcat + cagaattaaaaaaatggcaaacaccagcgagccaagcaatgtgcgaaatattattttaaa + aggcactttggaacgaaatcccgttccaaaagaaagttcattctcatcaacaagttcagt + atcaatgggttgaccattcgcgtctaattcatatagcggtataccccttctaaagttaga + accatccctactatctttcgcgtcgccaatcactatgggattgtttgtccacttcagtct + attcttataaaaccaatatttcatcctggtccacgcagatcgccaccccgatcttacagc + gtcttcatccgttatgaaatcatcacgaaatggatcttcactaaaatcattcagctcgtt + atcgagcgactctaaatcattctgaagttccatctatagagagtgttatactgtacctct + tttcagatatatgtaattacctcgcctcacttattttgcttatttgtagtttgttaggca + gtttgcatatcgtaccaatgtttttttataacttcatgtcttttcttttttttcattttt + agcatcgtacgcagatatcttgtctcgttatgatattttgtgagataggtatccaaaatt + aataagagaaaatgtcgtattgtgttcacaattcgcctgattgtttgactagtccaaccc + ctggtataattgtcacttaatatattcttagcttccaggtggtgttttattccattattt + atgttatgtatatataatcgtaactcttagagcaaaaagtaaaaaagaaggtggtactta + ccgagggagaacatatacaaatttattcgtaaatgtaaataattaattgaagcagagcta + aaagaaaaatacaagaaaatttatttacactcgctgtttggttctgatcttttggtattc + acaattttagtgctgtccaaaaatattgcgatgtgctgataaagtacatagtgtgatccg + acttcgtcccagtcaaagaaaggatgaaatattagttagtacatttactggttgctactg + gatgtcctatctgaatcttccatcttaggtacaaaaagattaagaaagtcaaattgtcct + tagttttaaaagacagagttacttcatgtctttaacatcaacagcaaattactttgaaaa + aatgcactaaaagttaacacatgtaggagcattctaaacatgaaatttttaatggtactt + aggtttgctaacgcatcttgaaaactagcaaaaaccccataattaaaatattcttgcttg + ttaaacgaggcgtccaagccattgaaaggttttatcaatattcaacctcttactataaat + gcagcacaccgtaacaggtatgagaaagatgtggtattattatacaaattgtactgaccg + ctttcctactaataaaaccaatgatatcctagggacgctcgagttttcacattatagaac + atttattattattttcggcatcaagccccagagttccttcgacacgttccatttgtttca + aaaggagcataaaagtttcttcaacattataatctaactcggcactgcattctataaatg + ctgcctggttcctcttatcttgactgccaattgtcgaagctagtttctctccttcagctt + tcgtgacacacctttttacaccttttgtactccttcccaaatcagctttggtacccacaa + gtattaccgggaggttatccttacccagctgatctaccagcttgtcccagagaatgggaa + taagatcaaagctagcacgatttattatactatagcacagcattatgcctcgtacgcccg + tcaacgatttaatgtttaatagagaaacttcatcttggcctgcagtatctagaatttcca + gagtacagtcatgacttttataaggaattatcctggtaaattcattttcaatagtgggat + aataggattcaacgaaccgcgattctacgaagcgaaccgttaatgtggttttgccgacat + ttctagctcctataagagcaatctttctctgaaagttatgtgtggagttcgaagaagaca + tagtggcgtattccattatgtcacaggttatatcttttctcgccgtgccccaataaaact + gtttcggtaaagagacaattcgaaccagcccaaaagaatgaatatgaaaatatcagtatg + cgtcaatgaaaatggaggcaagttcaataatttcactggaaatataactagtaataattc + gtgtaactgctgtagaaattgttagatattaaacctggcagaagcgtcttgttaatactt + atagagaaaccaccagtagcattcttctgtatctgaagatagtgaaaaatcaaaaaaata + aatctgtattcaaaaaaaggtcctacccggattcgaaccggggttgtccggatcaaaacc + gaaagtgataaccactacactataggaccaggacttcttgtagtatcaacgttctagata + ccaacatatcaatataaaaatactgaatattgagtcatcttttagattacgttttggttg + ccgcaaaccaaaagtgcaatttaaatgggtgaataatttgaatggttggaaatctattct + cggtaaaggttataatatcagtgttggaataaaactctaaaataccatccattcagaagt + attcatgctactagcattttatcacatgaggtgaggtgtaagaagatgacataaagattg + agatacagtcctcaaacctaatggaagcgaaatgcagggattgataatgtaataagataa + tgaaacataagaaacagaaggtagtagtattattatgtagaaatacccattccattttaa + ggattcctatgtcctcgagtagaacgtctagtatatacgaaatattataccctttaaaaa + acactggacccaacgataatcacaacattcaccgatttctcacctgaacagatcacgatc + atccttcagaacatcttgctgtagccatattaaattcctttgtctctggactctttctcg + cgcgactgatctcccaatgaagaattgtgtcaggtcatctcgtcctcaatttctaaccaa + aattggatgattttagttctcactgacgctgcttgaacgttaacaccgaaagagcatgca + ataatcacaacacggtaaaccaacttgtgtcatatttaagagaaatattaaaacgctaag + aagatagctcccataatagaagtacaagttatatttatcgttgcccaactgcagaagtta + aataaaggtaaataaatcgagctaagagaaaaaaaagtataggcaaaattccgaccggag + ctagctttaccttgctcctccacctaagtcacctgctcaggtcactcaagttcacaatct + tctgagaggatagataaaggaaaaattggaacagatcttttcatggcctggcagttgtct + gtggattggtaaagctctgtcagtgaaaaaaaagttctcgctccttcttggtctcttcta + tttcctgagattctaatgctttccttacattgtttcataaaataaccaatttttcgtgtc + acaataatcttgaaatttgaagtttctaaaatgcatcagacatggtgtcgtagttagttg + atttactttaacttctaccaagaagtagaaaatagcagagtgcgtgaacgcatagcagag + aacaaaatacttactaaacgttttcgaataagttagcaagaagcttttaaaaacatagac + ttagaaattgtcctgcacttggcaaggaagaaaagaaagattagttaaacagaaatcgaa + aaatgcatgatgaaaagctaagtagacctctttagaagtgtcttagcggctattgttcat + gatacttttgaaaagagccctgagattttccattaccatcaacaggaccaatgaatagct + aaaagaatcgaatgtatttatcggaaaggatttgaaatttcggccatttcctcattatga + agttcaacttccatactctcgtcgtcgccatcattttcattatcctggttgtcgtcatcg + tcatcttccccgggcatgtcattagcatctatataaggaacctgtttcttagacaaattc + tcttttatctgtagtgagcgtatgacgaccgagacaattccgcttgatattgccgtagcc + aagagggcgtagcaacctctctcaaacttgggcaccatatctgaagcaaagaataatata + gaccaccatgcgttaacggcccctgaaaacatattcattgaagcaagaacgatagcacgt + tcttgaagatctgcatgacaaataatgtttgcccacgaaaaaaaaaccgcttgtccagcg + tatgctacgcctccaagatactgtgcagaaaagacgacttttggatttagtgggtctgca + cgtattagtaccgcaacaataaccattaccaatgatatgaaaacagaaacatgccaatgc + ctagctcttgggatcttactcatatatacagcagaacaaagcgtagaaactatacctacg + gcaaatatccccgaaggataattatttctttgcgccaacgtatatttttggttttgtaac + cataatgcaaatgtagaattagatgcgaaacccaaattctcacctcccagaacccaaaca + agagagaacatccaccagtgccacctttttaggactctaggaatagtcgaccagtctaac + cgggtgctttcgtccctagcggggagccttctcctagcataatgtagctcttgttcatta + aaaatgtatctcgtcatagaaaatttgctaacagcacttgtttgatcaggaaggccgggg + aagaaaataaacccataaattgcaatgggtaatgtgatacaaaaatcaataatgaataac + catctccaacccgccaagccattccgcccattcaaatgagtaaagatacttgtttgcata + aatccactgaacatagatcccaccaaaccgctacctgtaaaaatagcacttctaatgggc + aattcgtcttctttataccacgaacccaaaacaaaatgtgttcctgaaaatgtgcaactc + tcaaataaggcttgaaagaatctaattgcgcaaatatgtttgaacgatgtaactttgtac + attcccaaggtcaataaaccccaggcaaacgtacaaaaacttagccatatcctaggtgga + acacacaataacattaaattattggggaccatacctacaatgtaaccaatcatgaaaact + gtgttagacacggtcaaatcgtttccgaccatcttaagatcttccttcatacccgatata + tatgcattggtgaaaccgacacggtcgacataattaatccagtattgcaagcaaacaaat + gatagcacaaacaagtctattttaaacagaagtaatctctttttaatggcacgtttggaa + gaaacagattctctctcaacctcatgttgagtgatagatttcgattccttcatcatgact + taggcccaggctctctccaatcaaaaattctgaaaacagcttctcaatcttgaataaatc + ggaatacttttatatcagaattacaggtccgcctcatttctcttaagagccgatgtcttc + ctttacaatatacgcatgtagaagatttgatcgatgctgagttcttgtaattcgtcaaaa + atgcctttcaagatcgattactcaaaatctacaataatcggactgcatggatcaatggag + gtgtagccaattttgtaacaatgaaggatatgttaaattcgctaaagcttcctcctcttt + tgctaaatttcatcatcaaaaagcattttgcataacacacgttaaccaatatattatagt + atatatacattctatttgagaataagcatagtttttacataactagttcgttattccaaa + tgtatttttcctatttgccataaatattttaagtaaaagaaagcatgctctagataaaaa + atatcgaggaagagtcgaaataagcaagcgtaaatattacaattattctgaagaagcagc + attttcttggccctcagcattttcttggccctcagcattttcttggccctcagcatcttc + taatttcttcccattcttcaataaattaatgtccttttgaactaagctcaaagtagtacc + cttagaaccgtcgtctctctcgaagacatagttagcagcatctgcttcaacatataccaa + agcgctgaataaattatgtaaaggaaatgttagtaaattataacgcgtaattgtaaagta + agtaaaattgaagaataaataacatacccttttctaacatattctgtcaaaaaattgatt + tgaggttcattgaaaacggtgatattataccaattggtttggccatctcttcttggttgc + gaagcgatactatatttcaaataacgattgttattagcagaagtatgttcagtgaattca + gagccaatgcggccgacgatggacattttcgagaagtccatcttcttggtagtagcatgg + aagaaacgagcttgagtacgtaaaaacattacaaaggattagtggatttttgtttgtttt + gtatttgtcaaggacacacaattagcaaatgacttgttatcaaattcgtggtatttgtct + ttctcaactactgtgttagcttttttttttcgcctgggcgcaaacgccttttaagcactc + ctccacaaataataagtattagatgaaaagtcctaaaagtccgaaaaaaggaatagaaaa + gaacgaaaatacagaatctttacagaacattatagtacataagtgtagttctacgtaagt + cttaaacaaaggtttcaaaaggaaaatatgatttagcggcgccacctccataaattatgg + attagcaaacaaaagaacaattaatattgaaaaatagaagcatagcagaaatatcaagcg + agaccatggtagttaccagtagtcaaagttctttttgtgtttacatcgagccactcacca + ccaaatggattgttttcatccagctcctgacaagtcaaggtgattccactgccgctgccg + ctatggggtagagccgcgttatccgtctcactgatagtgaatttggtaatcacgccttta + gcggattcatgtgccaggccgtctgtaataaatctggcgattaatctttgaccttcacca + tttcttgtcaaaacaactggttctttaaatctccacgttattctcttcttttctttactg + aaggagccctgaggttttgataaagcgcttgttgcattagcaccatcgatattcacaaag + acaacgaggtcttctattacgatttgtgatatttcatcgggtaagcttggtgacatcttg + acagtcaatacaacacttgcctggtgagattcaaatctccagaccggatgaataacaata + ggcgcgatcggctcctttatggaatatttaatagctcctaaggttctagaatcgataaat + gaagggttaactttgaattcttctggagcaacacgctcaataaaggcttggttcagaatg + accttttcgaacttagcgccattattgattctcaaattaataccgatagtaaaggcgaat + tcatgacagaattgggtaaatagttgagggcaatttccccaatcagttgggagttctgca + acataccgtctttgaaagaagcattgagcacttctgcgatactagcattcaacccaaatt + gagatgtatctaaagaggagtgctgaaacaacgattgaccaactaaagatgttgaagagc + ccgtggcttgaggatttagttctcttagctcgccagtaatctgagaggagagtgtggaaa + ctggacgttcctggggaatagaggacaaggaagaagaagtaggggtagcgcctgagccgt + gattaggcaaagagtcatagagttgagaatccctgttatgaatgaaattttgtttccttg + agggtggtaaagcaggtgcgcggatatgcaagggccttgaatcaacttgacctgaaggtt + ggttatcaaccgtagcagtaggaagtgaaatactgggagaggcaggctcaactggtaatg + gttttgtttttgattgtggctgaagtggaggttgagaaattgatatcggtactgtcttgg + gtaagttacttgagtctggttctttcgcagtgggtggagaactttgttgtggcgttgaag + gaggcacttcagaaggagtaaaagtttgcgcaggtggtgtatcggcagtaacaggtgcat + ctacgtttttaggagtagcatggggaggaagaggaggagtgtcactagcttcacccgggg + tccaattgtttgattttgatgatgaataggtgggtcgacgttgcttctgataaatagaag + aagttgcagttgaagacactctggtggaagaattattcggagtctcagtgatggatgcct + gaatatgagagtttgatgatgattgctgttgtctcttgttcttggtcttatttctaccga + aaattgagcccactttagatcttagggtgtgggacgacttttttgatttcaaggaagaat + tattagtagagtctgaaaattcgttattcatgagatcattatgagtgagactggaagatg + cagaggcaagacgatgtccaatgtttccaaatgcactcttacgcttatccttttgcgggg + atttcttttctctttccgtattagcggcaccattggatacagagaccgggcgggcaccag + tggcagaagctggcgaagcattattaggaactacttctttggaagaagaggatagttgga + agttgtattgactggcatctttggcaaaacgatcaatttcactttgcggttcgaatgcca + ggaatttcgtcatcaccgtttcgcattctttggtagtatttagtaaataatcgctaaaac + tggtttgaaatctaagcatacagttttttaaagtgtccaaacggttatagtcaatagtct + cgaacagctcaaacaaataggggctttgctggtcccattgccttcttgcctcttctaagt + gactagagttttcgttattcttactatagtgctcaattgaagctgcatttttactaagct + tggaatgtaagtccttgctttctctccagctagtattgttctccacggattccttcaatt + cacgaacgacttgctggtccaaagtgttgtagtattctgtacttgacttcaaatctgatt + ttagttcttcaattacagtatcccatacatttcttagctctcccagcgagttgaatctga + atgcactcatttcctgcggagtcaaaacattactttcaatcatttgagcgtttaagatct + ttgttatatcttcattttcagctattatttttctcaattgctgagcatagttcctcttca + gatttgccaattccttaaataacaggtaaaagtctttattcaacaatttgacctgcgata + acctgattctaatagtttcggtagcctcgtaaggactcttagtagtcaatatgctatctg + catatttggttctttgttccgtcatgagaggtgattattcgcaattttcaaataagcttg + cttcaatacgactacagcaaaagtaattcctttattaaggcaataactacgagacctttt + ctttgtatttcgtgaaaacttgaatgcaatatagagtttttcaatctgcaagtacttaag + gggcccttttagctacacataatatagaaatgaaagatcacgggctacgaaaagccattc + ataatcatatacaaatatcacctgtaatatataagaatatatgagctattgattttcttc + taccgcctgatttagttgttttcactcttcaaaactaaattcagtattagcgacagtgga + ggtgctagtactctgtaaatgaaagtaaacgctggtattcatgtagagcaccaacctgac + tgtgaatgcgtaacgtgaaacatgatagccgcctgtaaaatattgttatcaataagtaga + ccggcttacacgaataggattcgctttattctttactccccactcaaccctaagcaatgt + tcaaaatagtgtgagattattttatgcgcgcctccttcaaaaaaaatttacatatataac + ataggtggaaaaacttccctccgatgtctaataagttaatcaatagaatgaaatggacac + gaaaaggcgtagacatggaagatgacaatgacgagaacgtcatttttttttccacgtatt + ttaaaaaggggtagcctgcttcaccagatgttcttgtcaacaaaattctaaaatgatgat + ttttttaaacacaatttatgctagatagtatctgaaagcattcaaactttatgattacag + tgttttcgacagttttaaatctctgaaccgattttgcaactcttggtaattgtgtcctta + catttccgtagtgacgttaatcaaatcgcaatcttgaatcttcttaactaaaaggttata + agggcttttttttattactaaattaacgtatgagctgatttgattatgggcgctatataa + ttatatgtacacattttttaactgtaaaaaataatacggcaatacaagtacaatacatgc + ataactcataatcttctacctcttctaccacccttctttctggtggagtcagatggaact + ggggtaacatcttcgatacggccaattctcaaaccagatctggccaaagctctcaaagca + gcttgaccacctggacctggagtcttggttctagtaccaccggtagctctgatcttaacg + tgaacggcagtgataccgacttccctacacttagcggcaacatcttgggcagctaacata + gcagcgtatggagaagattcatctctgtcagccttaaccttcataccaccagtaactctg + gcgatggtttccttaccagataaatcggtaacatgaacgaaagtatcgttgaaagaagcg + taaattctagcaacaccaaaaacttgggaattgtcacgagcttgaacaacgtctaaatgg + aaatagtaaatcgatatcggacgatatcaaacgtaatgttgttagtatccaaaagttttt + ttttttaattcttacatagtatctttgcttttccatcgaaaaaaaagttgacaaaatata + ctgccattgacacaataggctctactaatcgctatgaattactttggaggcaatgaattc + gttgaattgtctttatgtttctgactttcaatgtatcttgcacgtcgtcagaaacattca + acaacaatcaatggaaaatagagatgctttaaaaatgttcactatgtgattaaacatact + agacatggcgggttcttgtggtttcttactctccttgggtttaaaatagaatcttcatta + aaaaaagtgctttctgtaatatatagtgattaataaccaattcatttcaactgtctacct + gaggaagccctgcaccatatggaatgagccaggaagcgggatggtctgccgcaagcagac + aaatttgccaaaatgcagtagagaatggcaatgctcgtgctgaaggttaaaaaaagaacg + caaaaaaaaaaagagtagtaaaatgtacagatgtttattaagtccgtacaccgcacgtaa + gtactccaaagcagtctctattacattttaaatagggagaaagaagatacataacaagta + tgaacaagaacgataatcagtgagtaaaatgaatagagatgtcaggcccgtgtgaagtac + ttgaaagtaataagtgagtatatagaatataccagaacttgtaagctttcgaataacaaa + aagagccatttgtacttattctcgaactcggttgtagagaggacgttgccttgaaagtca + caaatcccctacaataataccggtccttggaatgtaaattattttgttaattttcttata + agttcacagttgaaaaaacaacggtcacagtgaatcaataaaagaaggatctcactttat + aaagaaaagaacaggaaaaaaaaatgtatatctgggtatattacccggcccttgataata + tttttcaataattttgccaggtaacaatgtgattcatgcatggcttaaagaaaatacatt + gctgaagcaaaagctcaccaaaaatttgcgttgcaaagctttgtttgcggtatgttaata + aagttaccatgaagcttttttcatgctctcagaccataaagacaggaagcaacttgtcat + atctatttcgacttcagcggttattttcttcagcccattgataaggcgatatatgcctag + aagaaaggaagagtgcagacttgataataaagcaagaccaagagcaactaaatagactaa + aatatagagccttccaaactgttagttattattacttaatactcgtacgggtgatgaagg + tagtgactttgtaatccgggtaatggggaaaataagaagccttcaaagctatagcatttg + tccctactgcatcggcccctttccagtacaatagaatctcgttgatgtgattactgattc + aactgacccaaataaatcatttgaagaactcataaaaggactatagcagcaagaactgca + agccaattggttctttctcctttctgccaaaaaaaaaacacaatacggatatgaattcaa + ttattaatgctgcttcgaaagtcttaagactccaagacgatgtgaagaaggctactataa + tattaggagatatactgatattacaaccaattaatcacgaagttgaaccagatgtagaaa + acttggtacagcatgaactaaccaagataatacaaggttatcccatacaggataatatga + ttattaatagcaaaaaaggcacagttgaagatgacttatgcgaactcaataactatacct + gttttgcactttcgaaaagctttgatttatgccatgatagcagaaatttcaacatagcgc + agccgaaacgatggatacaattattagagacattaactgactcagttagtttcgcagtta + ttgttcaaattattctcactttatctaacatttcgctaataaataaacaaaccttgggga + agttaaaaaaactgaggattcgaattttcgaaatactatcaaataaaaacgatagttgga + aatctacattactacagaaaaaccttatagaatggtacatttttatgctttccgtggatt + gcacacctttagaattgcaaaacttatatctccataaggagttgaaattctgtaacgata + tcttgaattcattaacactccaagtttctgatcctcgctcacaaaattacctgcaatttg + agaacacgtataagctttttcaaatacaaaagtcatctagaattaacaactcgttccttt + tttacatagaattcaattccgttacctcaaataggataatgaccatagaaaaacacattt + atttggaaattaaggaaggccagttttgtatttcaaatgataactacataatcggtttat + ttgaaaacttcgaattcgaagcgggcactttgtactttattggagttttaattgatcaca + ataatcgaataactctttatgttgatggaagtatgatcaatcagctcacgttatttgaaa + actctatatgccaattaagcacttgtgaactgggatccatgatttgttcaattaaagtat + atagattttatttgtgggatggattattaacagaatttgcgataaatatacttcaagcta + tcggcaccaattaccaatatacatttagcaagaaaaaagaagggcctgaagttttatcgc + tctgccaagactttttgatcgctaaggctcatttaatggccaggcctgcaacagaaatat + cttccacaaaatacatcgatgagattgaacttcttgaaatggaaaatatcattattgatg + ttaacccaaatgatattcttcaagatttcaccgaatcgtctaattttacggtaaaatttg + aggaaagcacaaactcgaaaaatattccggaagtgggtaagtgctatttctataggagtt + caaacttggtttcaaaatttgtgtccattgattctatacggcttgcgtttttaaacatga + cagaatccggtagtatagacgatctgtttcatcatgtatcacatctgatgaatcttttac + gaaatattgatattcttaattggtttaaaaaagactttggcttccctttatttgcttata + ctttaaaacaaaaaataacacaagatttatctcagcctctgaatatccaatttttcaatt + tattcttagaattttgcgggtgggatttcaacgatatttccaaatccataattctagata + ctgatgcctacgaaaacatagtccttaacttggatttatggtatatgaatgaggatcaaa + gttctctggcgtcaggcggattagaaattatcagatttcttttcttccaaatttcaagtt + tgatggaagcctctatttattctaagttcaattccaataaattcaatgatatgaatatcc + tagaaaaactatgtttaagctatcaggctgtcacaaaaagagaaaatcagaacagtaaat + ttaatgagctatcaaatgatttaatttctgtatttgttactttattgaaaagcaatactg + ataaacgacacctgcagtggtttttacatctctcatattactttattaagagaaaagatg + tacgttctacagaaattatacttcaagcggtagatcaacttttttcgttttacttagatc + aaggtagcgacgaaaatgcgaagatactttcagagattataccacttaagctaatgctga + tgattatggatcaaatagtggaaaataatgaatcaaaccctattacgtgcttgaatatct + tatttaaggtagttctgaccaataaaccgcttttcaaacaattttacaaaaatgatggtt + tgaaactcatattgactatgctttgtaaggtagggaaaagctatcgagaggagattattt + ctttgcttctcacatattctattggcaattataccacagctaacgaaatattttcaggtg + ctgaagacatgattggaggaatttcaaacgacaagataactgcaaaagaaattatttatt + tggctgtcaacttcattgagtggcatgtgattaattctaatgccagtgattcttcttctg + tattggacctgaacaaccatatattaagattcgtcgaagatctgaaatcgctgagcgctg + ttccgattaatgaatctgtatttgatcctaaaaaaagttatgtgatggtttcattattag + atctctcgatagctttgaatgaatcggaggacatctcaaagttcaagagctcttcaaaag + tgatttcagagctcattaaaggtaatataatgtgtgctcttacgaaatatgccgcttatg + atttcgaagtctatatgagcacatttttttgtcacagtacagaatacaaactggtttatc + caaaaactgtaatgaacaattccagttacttagagctatcatttatagtgacactcctac + ccgaaatacttaatgacctgatagatagcaataacaatttgaacctgatgatgttgaagc + atccatacacgatgtcaaatctcctttattttcttcgcaaatttcgacctgatacgtcac + agatagttatgcctaaagatttttatttctcaagttatacatgtctcttgcattgtgtta + ttcagattgataaatcatcattttaccatttcaaaaacgtttctaagtcgcaactgttac + aggaattcaaaatctgcataatgaacttaatatattccaatactctaaagcagataatct + gggagaaagaagaatacgagatgttttctgagtcactgatggcgcatcaggaagttttat + ttgcacatggagcatgtgataatgagaccgttggcttattgttaatattttttgccaaca + gattacgtgattgtggatacaacaaagcagtcttcaattgtatgaaagtgatcattaaga + acaaggaaaggaaactaaaggaggtggcgtgtttttttgacgcagcgaataaaagtgaag + tactcgaaggtttaagtaatatcctctcatgcaataactctgaaacaatgaacctcataa + ctgaacaatacccattttttttcaacaatacacaacaggtacggttcataaacattgtca + ccaatatcttgtttaagaacaacaatttttctccaataagcgttagacagatcaaaaacc + aagtttacgaatggaaaaatgcaagatcagaatacgtcacccaaaacaataaaaagtgcc + ttattttatttagaaaagacaacacatccttagattttaaaatcaaaaagtccatatcaa + gatacacttacaacctcaaaacggatagagaagaaaatgcagttttctatcgaaataatt + taaatcttttgatttttcatctgaaacatacactggagatacaatcaaatccaaattcgt + cctgcaagtggtcattggactttgcagaagattttgatgggatgaaacggaggcttttgc + ctgcttgggaaccaaaatatgaaccactcattaacgaggaagatgctaatcaagatacta + taacaggtggtaacagacaaaggagagaaagtggaagcattttatcctacgaatttatcg + aacatatggagactcttgagtcggagccagttggagatttgaatgagaatagaaaaattc + ttagacttttgaaggataacgattctattgcaactatttggaattgcagtttgattattg + gattagaaattaaggaggggattttaattcatggcagtaattacctttactttgtaagtg + attactattttagtttagaggataaaaagattctaaaattatcagaagtatcgcaagaat + cacgggatatgacggttagcttaattaacggccctgatgttaaaagggtatcaactttcc + taaagcacgaagtctttgtttggaaacttctcgatatcactttcgttaccaaacgaccct + ttctacttcgggatgtcgccatcgaattattgttcaaagagagagttagcgcttttttta + gtttttacaacaaaagagtgagagatgacgttttacgggtactgaataagatcccgaagc + accttccagcagatccaattttttcaagcgttttacaagaaataaacgaccgaggaaata + gtatagtggcaagaaatggaataggaaaggcaagcattgcttccaaattcactagcgtct + tctcagcgaacaacagcctaatagatggatttgagatcagcaaaaaatgggttaggggag + agatttctaatttttattacctgttgagtatcaacatcctagcgggaaggtcattcaacg + atttgacccaatatccagtgtttccgtgggttattgcagattacgaaagtaacgtactcg + atttagagaatcctaaaacttaccgggacctatcgaaacctatgggcgctcaaagtgaga + aaaggaaattacagtttatagagcgttatgaagctttggcttccctggaaaatgctgatt + ccgcaccatttcattatggcacgcattattcctcagctatgatagtatcttcatatctga + taaggctgaagccctttgtcgaatcctttttgttattgcaaggcggaagttttggccctg + cagatcgtttatttagttcgcttgaaagggcctggagctctgcttcttctgaaaatacaa + cggatgtcagggaattgacacctgaatttttttttctacctgaatttttgatcaacgtta + atagttatgactttggtacagaccaaagcggtaaaaaagttgacgacgtcgtacttccac + cctgggcaaatggtgacccaaaggttttcattcaaaagaatagagaagctttagaaagtc + cttatgtatcagcacatttacatgaatggattgatttgatatttggttacaaacaaaagg + gggaaattgctgtgaaatctgttaacgtattcaacagattgagttacccaggcgctgtaa + atctagataatattgacgatgaaaatgagcgcagagctatcacaggcattattcacaact + ttggtcaaacgcctttacaaatatttcaggaacctcatccggaaaaaatagcctgcaatg + ttcaacagctaacaacagaggtatggcgtaaggttccaatgaagccaatatttgagaaga + caatctttaatttgaatgaaaagaacaggtctgtcgattatgttatacacgatcctagtt + acttcgattcattatactggaggggcttcgctttcccaaacttgtttttcagaacggaag + aatcgttagtgtcattgagaattgtgcataaaaattggttaaaaattggactagatattt + ttaaaaagacgcatatggctcagattacatcgtttgcgtactggaagttgggcgaattca + taactggtgataaaaatgggctgataaaagtttggaaatatcgtaaagataagcattcgg + tttcaggtaaccttgagaacaaaaaaacaatgtttgggcacctatgcgagctaaaggaaa + tgcgctgttatcacgactacaatacgcttttaaccttagacatcagcggcttagtatatg + tctgggacatgattaatttcgaactagtgagacaaataacaaatgatgcgcaaaaggtcg + caatatctcaacatgcagggagcattatggtattgactaagaataacgccatttcgatct + tcaatctaaatggacaaatatatacatcaaagaaattcgaaccagctaaaattgtaagct + caattgatttttttgacttcactaagttagacgcaggttacagaaagcatatctattgga + aagagatggaaatactactagtgggctttgaagatggaactatagaaatttacgagctct + ttttgacttttcataatgaatgggcgataaagctactgaaacagctctgtaccgaaagag + ggaaagccataactagcattaagggacaggggaagacatacctgtcccagaaaagacgca + aggatacagcagagcctcatgagatagaagtgattgcgggaacattagatggcagattag + ctatttggtactaggcatgacatcgtaacgcctttctttaaatgattcaatttttgtagt + ttatatctttacttttgaaactgatttctcatcccacctagtattgtaattgcgtacgta + tccaatatcattaccaacgccgggtatttttttctagtatttcttctccatttcgcctat + ggaaaacagcaaaagggtaaaagaaaaaaacaaacgattaattcttcattgaattatgta + aaaatcaaaacgcaaccgcagatttaatagagaccagaaattcggattactattgacttt + gtgcaccaccttcaaatttactcattgtttaagacaggcagtgggaaagaagccgtcata + ttgctcgaatccttaacaagcaaaatatacaaccactaaattattccgaaagggcctgct + taataatttgcctactaacttgtgcatagaacagcaaacagaaacaaagcgtaagaaaca + tggggtatccgccacctacacgaaggcttggagataagaaaaggtaccattattccaata + atcctaaccgaaggcatccttccgctgtttattccaagaatagctttccaaaatcaagca + ataatggatttgtatcttctcctactgccgataattcaacaaatccgtctgtaactccca + gtactgcatctgtacctcttcctacagcggcacctggaagcacgtttggtatcgaagcac + ccaggccatctcgatatgatccgagctcagtcagtaggccttcgtcatcatcttattcgt + caacaagaaaaattggaagccgttataacccagatgtggaaagatcctcttcaaccacta + gttcaactccggaaagtatgaatacgagcaccataacacacaccaatacggatatcggaa + actcacgctattctcgaaaaaccatgagcagatataatcctcaatctactagttctacaa + acgttacccactttccctcggcattatcaaacgctccaccgttttatgttgccaacggga + gttctcggagacctcgatcaatggatgattatagtcctgatgtaacgaacaagctcgaaa + caaataatgtttcatctgttaataataacagccctcattcttattactctaggagcaaca + aatggagatccattggaacgccttccagaccaccatttgataatcatgtcggcaatatga + cgaccaccagcaatactaactcgatccatcaaagggaacctttttggaaagcaaatagta + ctactattttaaaatcaactcattcacagtcatcgccttcccttcatactaaaaaatttc + acgatgcgaataaattggacaaaccagaggcttcagttaaagttgaaacacccagtaaag + atgagacaaaaaccatatcgtaccatgataacaattttccaccaagaaaatcagtttcta + aacctaatgcacctttagaacccgataatatcaaggttggcgaagaagatgcattgggga + aaaaagaagtacataaaagtgggcgtgagatagcaaaggaacatcctactcctgtaaaaa + tgaaagagcatgatgaactagaagctcgcgctaaaaaagtaaataaaatcaatattgatg + gaaagcaggacgaaatttggacgacagcaaaaacagtggccagtgcagtcgaagtttcca + aagaaagtcataaggaactaacacgctctgttgaaaggaaggaaagtccagaaattagag + attatgaaagagcatacgatccgaaagccctgaaaacagacgcaacaaagttgacagtag + acgatgataataaaagttacgaagaacctcttgaaaaagtggaagggtgtattttcccat + taccaaaagcagaaacgagattatgggaattgaaaaaccagaaaagaaacaaaataataa + gtaaacaaaagtacttactgaaaaaggcaattaggaatttctcagagtatcctttttacg + cacagaacaaacttatacatcagcaggctaccggacttatcttgacgaaaattatatcaa + agataaaaaaggaggaacatttgaaaaaaataaatttaaaacatgattatttcgatctcc + agaagaagtatgaaaaagaatgcgaaattttgactaaactgagtgaaaatttaaggaagg + aagaaatcgaaaataaacgtaaagagcacgaattaatggagcagaaaagacgtgaagaag + gtatcgaaacagaaaaagaaaaaagcttacggcatccatcctcgtcttcctcatctcgtc + gcagaaatagggctgacttcgttgatgatgcggaaatggaaaatgtattgctacaaatcg + acccaaattataaacattatcaggctgctgcaacaattcctccgctaattttagatccaa + tccgcaaatactcttacaaattctgtgatgtaaataacttggttacagacaaaaagcttt + gggcgtctagaatattgaaagacgcctctgacaactttactgaccatgagcactctttat + ttttggagggttatttaattcatcctaaaaaattcggtaaaatttctcactacatgggcg + gcttaagaagtcctgaagagtgtgtcctacattattatagaacaaagaaaactgtgaatt + ataaacaacttcttatcgataagaacaagaaaagaaaaatgtcagccgctgcgaagcgcc + gcaagaggaaggaaagaagtaatgacgaggaagtcgaagttgatgagagtaaagaagagt + caacgaacacgatagataaggaagaaaaaagtgagaacaatgccgaggaaaatgttcagc + cggttctagttcaaggttctgaagtgaaaggtgatccattaggtacaccggaaaaagttg + aaaatatgattgaaaagagaggcgaagagtttgcaggtgaattggaaaatgctgagaggg + taaatgacttaaaaagggcgcatgatgaaattggagaagagagcaataagtccagtgtaa + tagaaaccaacaatgaggtacaaataatggctccaaaaggaggtgttcggaatggttatt + atccagaggagaccaaagaacttgacttcagtttagagaatgcgttacagagaaagaaac + acaaatctgcaccagagcataaaacaagttattggagtgttcgtgaatctcaactctttc + cagaattgttgaaggagtttggctctcaatggtctctcatatcagaaaaactgggtacca + aatctactacaatggtaaggaattactaccaaagaaatgcagctcgcaatggatggaaat + tactggttgatgaaaccgacttaaagcgagatgggactagttcagaatctgtacaacaat + ctcaaattttgatacaaccagaacgaccaaacatcaatgcctatagtaatattcctcctc + aacaaagaccggctttgggttattttgttggacaaccaactcatgggcataatacatcta + tttcatctatcgatggctctataagaccatttgggcctgattttcatcgtgatacctttt + ctaaaattagtgctcctttaaccactttaccaccaccaagactaccatctattcagtttc + ctcgttcagaaatggcagaacctacagtgacagatttgcgtaacaggcccttagaccata + ttgacacgttggctgatgcagcttcgtcagtaacaaataatcaaaacttcagtaatgaaa + ggaatgcaattgacattggccgtaaatcgacgacaatcagcaatctattgaataattcgg + atcgaagcatgaaatcttctttccaaagcgcttcaagacacgaagcacagctcgaagaca + ctcccagcatgaacaatattgtagtacaagaaataaaaccgaatattactacgccaagat + cgagttctatttctgcattactaaatcctgtaaatgggaatgggcaatcaaacccagatg + gaaggccgttgctgccatttcagcatgctatttctcaaggcactcctactttccctttac + cggcccctcgcactagtccaataagtcgtgcgcctccaaagttcaatttttcgaatgatc + cgttggcagctttggctgcggttgcctccgcgccagatgcaatgagcagttttttatcta + aaaaggaaaataataattgaacaaacggctgagacgggcaatacatatgctctacttctt + ttccatccaatggttggtgaaactctcgagcatacattaccttacgtgtgttagtgtact + atattatatatatatatatgtatatatataaagggaggagtttttaattataattgtaat + ttcgtattttttctgcattatacagttttttccgattttaaacgactttatttaagtgtc + gtgtaaatatgtcacattttatttttgtacgtattcacatgtcctggcgtgcggccattg + ctgaaaatcgcaaaacccacagagaaataaacatcgcgaaaaagtcaatgaaaaattgga + aaatatttttcatttcactattatccacaagcaattttgtacaaagtgaaaaggttgaac + taattatcttcgtctagaagccatgaattcactcgttactcaatatgctgctccgttgtt + cgagcgttatccccaacttcatgactatttaccaactttggagcgaccattttttaatat + ttcgttgtgggaacatttcgatgatgtcgtcactcgtgtaactaacggtagatttgttcc + aagcgaattccaattcattgcaggtgaattaccattaagcactttgccccctgtgctata + cgccatcactgcctattacgttattatttttggtggcaggtttttgttaagtaagtcgaa + accatttaaattaaatggccttttccaattgcataatttggttttaacttcactttcatt + gacgcttttattgcttatggttgaacaattagtgccaattattgttcagcacgggttata + cttcgctatctgtaatattggtgcttggactcaaccgctcgttacattatattacatgaa + ttacattgtcaagtttattgaatttatagacacctttttcttggtgctaaaacataaaaa + attgacatttttgcatacttatcaccatggcgctactgccttattatgttacacccaatt + gatgggcaccacatctatttcttgggtccctatttcattgaaccttggtgttcacgtggt + tatgtattggtactatttcttggctgccagaggcatcagggtctggtggaaggaatgggt + taccagatttcaaattatccaatttgttttggatatcggtttcatatattttgctgtcta + ccaaaaagcagttcacttgtatttcccaattttgccacattgtggtgactgtgtgggttc + aacaactgccacctttgcaggttgtgccattatttcttcatatttggtactatttatttc + attttacattaacgtttataaacgtaaaggcaccaaaaccagtagagtggtaaagcgtgc + ccacggcggtgttgccgcaaaggttaatgagtatgttaacgttgacttgaaaaacgttcc + tactccatctccatcaccaaaacctcaacacagaagaaaaaggtaagtgtaaaatctttg + aaagaatttaagtattcaactttcgtatattcgttttttcttagtggatctattgttact + attatcactattattatattgtaaaagaccggatggttttgttatatattacatacacat + gttatcgttgaaaaaagttttccgtttcctttcgacagtcatcagataattttatccgag + tcttttatatgttaaatcttgttgacaaatcgtccgctctaaccctagataacaacaatg + atctttttatcatttctggcgttattttagcgcctcctcccattaatgttaattgcttat + agttacctgaaggagcagcgaggatggagatcgtactgtgtatacttgtttcttccgctt + cagtatctaggtctgctattagcacagtgttcaatttatccatatcaatgtccacttcct + cttcttcagagttatcagagttttgtaattggcattcggggtctaactccactatcccat + aatttgaagcaaatgcaatattctttgcgtttatcattaacggtactgacttagtttggt + cgcaaataatttcatacgtttctcttatggtggcacttcttcctcttgttcttatagtca + ttcttaaatcggacgcacgctcatctataaatgctctaggtaactttacgctctgtaaag + cgtacatcaaagaattccaacacaaatcgaagactggaccagtgcgactcaagactacaa + ttttcgcatacaaaacgtatgaccattttcttttcattttcaggtttgtttcattcagcg + tatcatcttctaattcatcgggatacaagacggaaaacgtaccgtcttcattcgcactac + gaacaccagcttttaccttcaaggcttttttaggcaatatcctagagtgcaatatggaat + cgtgtagtttttgtgatatagtcatttcttcatcggtgcaagcgcccactctacctcttt + caacttccaccaccggatagacagaagcataatttgcaattatgtcctcctctttggtca + cttcgaacaattcttcctcaccgaaatcatccaaatctttaatcgaggcgctagtttctt + ctattattccacccgtaatagaagttatgactatggttttcccacttttcaaaacatttg + agcctaaaatgttgtttttagtgtctatattaccagcatccgcataacgcgataaagtat + tattttctatagcaacatctctaaattcttcatattttcttagacatggcctgattccta + gggataagtgtctttgtaaagacaattctggagatattctcgcaagaacttcaggtggaa + aagtaattgggtgtatctcaatagtttctaacgtggtactttcagccatttccaatatta + gatgcctgtgaaactactgtcaaacttatttgtgtagcaatactcatcgtctaatttgtc + cattattgttatatactatgtcataaaaaaagaaagaaaaatcttatactttcgtcgact + gtgatttgaaaatctctccagatcaaacgggaataaaagggtttatatccgaaggtggcc + agaacaatcatgaagcaaataggataaaaatagccaataagatgtttgttgtctttcatc + ccaaagttccaacagcatatttcaaagatgggtattacagtaataggttctctaaactat + gatttggacacatttacggatagattacctaacgctggagaaactttcagggctaaccac + ttcgaaacacatgctggtggtaagggattgaaccaagctgcggccattggtaaattaaaa + aaccccagcagcagatatagtgttcgaatgattggtaatgttggaaatgatacatttggt + aaacaattgaaggacactttatccgattgcggagtcgatatcactcacgtcggtacttac + gaaggcattaatacgggtaccgctaccatattaattgaagagaaagctggtggccaaaat + aggatattgattgtagaaggtgctaacagcaagactatttatgacccgaaacagttgtgt + gaaatttttccagagggcaaggaggaagaagagtatgttgtttttcaacacgaaattcct + gatcctctttccattattaaatggatacatgcgaacaggccgaattttcagatcgtatat + aacccctcacctttcaagaccatgcctaagaaagattgggagttggtagaccttttggtc + gttaatgaaattgagggtcttcaaatcgtggaaagtgtatttgataatgaacttgttgaa + gaaataagggagaagataaaggacgactttttaggagaatatcgtaaaatttgtgagctt + ttgtatgaaaaactcatgaatcgaaagaaaagaggaattgtggttatgactttgggttcg + agaggggtgcttttctgttcgcacgaaagccctgaagtacaattccttccggctattcaa + aatgtttcggttgttgatactacaggagctggagatactttcctgggcggtttggttact + caattgtatcaaggagagaccttgtctatggctataaagttctctacattagctagttca + ttgaccattcaaagaaaaggtgctgctgaaagcatgccactgtataaagatgttcagaaa + gatgcataaaatctactgcaattttttacttttagtttgttaaattttagttttcgtcat + atatataaagtttaaaaaggtacaaagaatgcagtaataagaaatgtaattacatgaggg + cagtttaagtgctaccttttaagacggaagaactaataccataaccgagagtaattacgc + agacaaacgctaacaacgaagcaggaacacctctggagataaaagcgcctacagttagcc + atctattaccctttttatcggtcatagaaatagcagtaacgtttggaaatccagatgaag + ccaaccccataccgcatgaggctaacaaggcgcaaccgaacacaagaattggagctgcct + ttggatcggataatttgtcaccaacttcttgcaccaagggaataatgatgattgctgaca + cagtatgtgagacaaaagtgcccacaactaacattaaaataccgaaaatacataagatag + caaaaacaccatcgttctgaattttcttttgtaatgctcttgcaatagttaccaacaagc + ctgaagatgaaactgccttaccaagggctataccacccatagcaagaacaacaattgacc + aagggaatgtgttgaaatcctttgttgatagtagacctgtaccaaaaaacaggactatcg + gtattactgcaatttcaccggacgatccaaaagcactttctatttgtgactctacacacc + atagaagaatagtagcaatagttacaatgatgataaaatattgctttatagtaaatctgg + tccttattggtttaaatttttccagtttagttttgcctattttaaaggtgagtatcatca + aggcccaggagcacagcatcgatagaatacctgtaggcagagcgacagcaaaaaattgcc + cccagccgattccataaggttttaagtactgcatggagatgatattctgtggcgaagaaa + taggtgaagccataccaccaatatctgccgaaagtgcaacacccatgactaatgccttag + caaacggtgaagtgtagtccagcggatctagtaagggtgttaatagagaatatgtcaata + ctggggaggcaacgttggaaatccacatcgaaagaaagaatacaacactcattgccatta + aaaggacatttcttggcttggtacctgcaagggccaataaccacgatgccaaaactttcg + caacgttatattgcgacaaggcttcacccaatgtgaaacctgctaataaaatcataattg + ttgacgaccacattgtacctaagatttctgtagaggcagctgctgctcccattaccttac + cgtcatcgtcttttagtaccctaaaaaggacagttagaaggggaaccaataaacctgtga + tgtgtaatggaatggcttcactagcccataagaaagcacagcattctaccaatgccatac + accggtgttcgacagggtcattgaaagttttaacacccaacaaaacacctgtgacaatga + taatgaagcctattttcattgctcttttcccaaaaaatagcttaggaactgccaaatttt + caattttagtaaacttcaaattaattggcttaggcaatggatacttgaaatattcaattt + gaagcttttcttgcataagtttctcagcttcatcttcaatagttattatatcgttatttt + gcgaagagaggcccaacatgtctttccatgtgttgcttctttcccaaacaatgtggtcgt + gcaggtacgactttaactcttgcttgcatgaatctacgttcccaggctgacccatgagaa + cagcataaaattcaataagttgtgcaattttactgtttaaactgcttaaagtttcatgct + ggaagatgtaggtgtctttgaaaaattcttcactttctattaattcttgcctagtgttca + tgtgcaatactttatcagacttcttagtaattttactgaaacccattctgttcaattcga + taaaagatttcaactggcaaaggtctatgtaaagatttattattgtctgttttaataatg + attttttctgagatttaatgttaaattgcgaataattcaagagggcggtattgtcggtag + tgtcagcaaacacgtcaacgtcatcatcttcatccatttcttcttcgctgtaatcaaacc + tatttctcaattcactgtcgatgtttgaagagtgtatttcaaatgggttattagatgaat + gtgtctctgatgtaccagaaataatttctcttaaaggaggcaatgcttgaatttcaggat + tgaaagtttcattcaatcttgttacaatgccttccttctccaaatcattaatcaagtggt + caaatctttcatagaacttagcttccattcttttgtagaaatcatctactttcaatttct + cgtcgctcaggttagttacgaatgtatcataagaactgaagagcgtctttccctcgctac + tcacagatgagctgcgtgattcaaagaatttcttacgtataaaattcttattgaaagagg + tggttttcccttgaggagataactcgtctaactcaattgtttcctcgttaatattgttcc + catctatggccttttcgtcggagtctccccttttgcttcctgaaggtgttttagaaccaa + agagcttacgcttgagtttatgtgtaaatcttcttttcgatggagatggttctcctgcag + ctatgttgctttctatatcagcgattggacctggagtctgagagtcagcgtcatcgttta + agtcaccggttggcgtttcttgtttcaattcatctgtctgtaatgtgtagatcaaatttt + tcaattcgttataatctaggtaatgattctgccattcagggacagcgttgtatttgagaa + agtgtgagaatctcatttcgaggcacaataatagccgcctttaccgtagttttgctgcac + ctttatctgagagctgactgcttttttggtgtgaaacactgttttctggtaataattttt + caatgcatcggattacctttcccacgtgcgaaatcatcaattaattagattgaaaaaagg + gtaagggaaaataagaaagaggcggtagcctaaagatacggtaattgaaacgtttcctat + gcacaatcttaaacctttttaggtaattgattaagttgactgtaatatctgtaaaagatt + acatctaatttacgtctggtttcttatcaaaaaatattatctactgtagttatgcaagta + ttcttaactttcagtactaaacatgaatattaattatttgtgagcgtacaagaagcaaaa + ggaagtcatctttctttgaacagttctgtttttaggtaagccttggaaccttagctatac + tgacttgatacgccctgtctttgttgtcgttatataaagtgtgcacctttatcatagccc + ctaataagcttcttgcagtagtatttgaacagtgtatatcttcatattgcttttgttgta + aatattgcgcaaaagcgactatctttgtgatttggttgaacttttgcatattcacgttat + taccatctttagtgaatgtatcgtttccgtctcttatgaaggtaatgtccctgataagca + gtgatgtaaaaggtacacaaggaagctgactgtggaaaatatgtttaattgttcttctat + aaacgttgtagttgttgtttggatgtacgacgacttttaggcgctgaaaaaggtggtctg + atttaacatctatcgggagagatagtctttctattgaatgattttgcaatgatgtaatga + ttgaagcaagtgagtttaagtttcgtaagtatagacaagcaagtgcaacttgtaaccagt + aggaaatggtgtgcgtttgctgcaaagttgtctcaagaacgtaggacgataactggttgg + aaagcgtaaacacggagtcaattgttgtatcgttgtgtttgaaatgtcttgtgaattcta + ttgtttcaatatccaagtaaagagaagattctaagagagtcaatgtctttgcaagtgacc + atggagatacgtttagagcgaaggcttcgacttctagaggttcaccctgtttccattgga + aaagtaaatcactgaggtcagttgcaccgcacaattcatcatttgcgttcgttttgtcta + tctcgtattggagaaataagttttcgtagtccaggggcggtttattttcttcgatttcct + tatcagttagaccaatgtaatgaatcttcaattgcctcgttctttttttgtttataaaca + gctgaactatgtctgcaaacagttcttggtattcataatattcagccaagtgactgtacc + aaaaattgaggatatcttttaatgttgtctcactatcttgccaataagactctacccaga + tttgtattagacgagggacggagtgatttttgtgtttgtttttattaattgtgggatagg + atagtagcaactcttggaggagagcattgtcagttgtccagtctctgaagttaagtagta + agtttgcggagtcaaagggggatggcttttgccatttgtgagagttgtgcggcagcatct + tattcaaatagagctgtattctgaagacctcttgtagaacatcatccatactaaaaagta + aatcgtcctgtcccattacgagctgtattagtgctgtgaccctctgtatatttacgttgc + catgaagaaggtaatgggcgatattttgatacaattcctgagttgcatgttggattgagt + ttacgaagggtcgccagacggccagaaacctccaggcggagttaacaactaataatacgg + catccatgtttgcatcagcgccgagcctataccagtcactgagtagacgttttcttgctc + tttttatgtcctgacttcttttgacgagggggcattctctagagacacaggcagttgctt + ccagcaactgcggtacggccgttctcatgctgtcgaggattttttttgggacgatattgt + cattatagggcagtgtgtgacttatgaattgttgtagaaggacgtctgtgatgttggaga + tatgtattttgttaactcttcttgagacgatttggccctggatagcgaagcgtgcggtta + caaataggtcgtcttgttcaagaaggtaggcgaggacattatctatcagttacaaacatc + ttagtagtgtctgaggagagggttgattgtttatgtatttttgcgaaatatatatatata + tattctacacagatatatacatatttgtttttcgggctcattctttcttctttgccagag + gctcaccgctcaagaggtccgctaattctggagcgattgttattgttttttcttttcttc + ttctattcgaaacccagtttttgatttgaatgcgagataaactggtattcttcattagat + tctctaggcccttggtatctagatatgggttctcgatgttctttgcaaaccaactttcta + gtattcggacattttcttttgtaaaccggtgtcctctgtaaggtttagtacttttgttta + tcatatcttgagttaccacattaaataccaacccatccgccgatttatttttctgtgtaa + gttgataattacttctatcgttttctatgctgcgcatttctttgagtaatacagtaatgg + tagtagtgagttgagatgttgtttgcaacaacttcttctcctcatcactaatcttacggt + ttttgttggccctagataagaatcctaatatatcccttaattcaacttcttcttctgttg + ttacactctctggtaacttaggtaaattacagcaaatagaaaagagctttttatttatgt + ctagtatgctggatttaaactcatctgtgatttgtggatttaaaaggtctttaatgggta + ttttattcattttttcttgcttatcttcctttttttcttgcccacttctaagctgatttc + aatctctcctttatatatatttttaagttccaacattttatgtttcaaaacattaatgat + gtctgggttttgtttgggatgcaatttattgcttcccaatgtagaaaagtacatcatatg + aaacaacttaaactcttaactacttcttttaaccttcactttttatgaaatgtatcaacc + atatataataacttaatagacgacattcacaatatgtttacttcgaagcctgctttcaaa + attaagaacaaagcatccaaatcatacagaaacacagcggtttcaaaaaagctgaaagaa + aaacgtctagctgagcatgtgaggccaagctgcttcaatattattcgaccactcaagaaa + gatatccagattcctgttccttcctctcgatttttaaataaaatccaaattcacaggata + gcgtctggaagtcaaaatactcagtttcgacagttcaataagacatctataaaatcttca + aagaaatatttaaactcatttatggcttttagagcatattactcacagtttggctccggt + gtaaaacaaaatgtcttgtcttctctgctcgctgaagaatggcacgcggacaaaatgcag + cacggaatatgggactacttcgcgcaacagtataattttataaaccctggttttggtttt + gtagagtggttgacgaataattatgctgaagtacgtggtgacggatattgggaagatgtg + tttgtacatttggccttatagagtgtggtcgtggcggaggttgtttatctttcgagtact + gaatgttgtcagtatagctatcctatttgaaactccccatcgtcttgctcttgttcccaa + tgtttgtttatacactcatatggctatacccttatctacttgcctcttttgtttatgtct + atgtatttgtataaaatatgatattactcagactcaagcaaacaatcaattcttagcatc + attctttgttcttatcttaaccataaacgatcttgatgtgacttttgtaatttgaacgaa + ttggctatacgggacggatgacaaatgcaccattactctaggttgttgttggatcttaac + aaaccgtaaaggtaaactgcccatgcggttcacatgacttttgactttcctttgtttgct + agttaccttcggcttcacaatttgtttttccacttttctaacaggtttatcacctttcaa + acttatctttatcttattcgccttcttgggtgcctccacagtagaggttacttccttttt + aatatgtacttttaggatactttcacgctttataacaatatcaagtttaccttcttcatt + actattcatcttcgccacaagtcttctctcccttggtgtttccaatctaactacaaaact + gttgattagggtgtacatcaccctaacaagatcatgtatttgcttcctctggtacaagct + aagaacaggtaaattcaaaacatcccagagtaatatcttcaaagggctataccctttaaa + catatctcggcatatttgtattaacccactaatattttgacggccaatcttttctatttt + tattttcatatcatcgacgtaatgaccacttaaaaacaactcaatatcatcctcactttt + tatatttttaacttcattgtgcacagattggacggaggacttaatatcgtcacttatggt + atccaaactaccatccataacaactagatttacacattcagaaagcacatcaacactttt + atccctaatataagaatcctcatggaagcaaagtatttcagtaaacaaaacaagggcatc + cttgttttttaaaccaccttctaaaagcatgattttcaaaaccattaaaaactcattctg + aaagctttccctggaaatatctccgttatttatacctaatgttaatgtatgcattatcat + tcctgtcaattccgtaaagtcataagcacccactttatgcaatttgtacttagcatacat + gatagtagtcttaatcaaagactcgtatgatgaaagccaattcaccaaattttcgtacct + caataactgagtcttaactttttccatatatgctttatctttatactcatttaaagcaac + tttaacaacattttcaatcaagctgcggacgtacgcatcatcactgtatttattttcacc + attctcattataaacaagaatatccagtaaaaattgcttcaccaattttggacatttacc + attttcatttttcactttagccaaatatttgggaatattacactgtaagaaatacaactt + aggattctcaaagttattactctttggaatattagagtcttctaggcaaaacaaaatctg + aaaaatctgaattaaatgcttaacaccgccagtaaaatccggatcatatacgtatttact + aagcgcttcgcacgccgcgagtcttatgccaaaaaagtaacgctcgtcgatcgccgttct + aaacaaaatacttgaataaacaagtgatttcacaccaccattaacaacgacgtcctcata + atatcgtatggcttctagttgggcctctatgtccccatcttgtctcaactgagaagaaaa + catgtaatccggctgattaatgtgcatttggcaaatccattccagatcagagtctatgcg + tatccactcaaatgcttcgttttgcttaagcaattcattactctcagacgtacgattaaa + ttccgtcaaactgaatcgggaacactcttcgggcgacatgtatacatttcctagacaatt + cacatccacaacaataggcttctcctcattattgttttcaacaccattttcatcatttgc + accaccacctcttttccttaatcttctgtactttgtattgtactgaatatctatttttgt + gaatgtatctttgatttccacaatatgctcatacggagtaccatcgtgttcatggatcct + tatagtcatcgagcccgtgaagcattcggttcggttcaaatctggatgttctaagtggtc + tagtgcacttttgaaaaatccttcctcccctaccactttttcgtggccaagttcttcatc + ttgaacttgccttatacccagttctataaccatcctcttcctattaaatctttgggtgac + acgtaatatgggtaccccagacccatatacccattcgttgaaaaaattctctaatttact + tttattaactctttcgcaaacatgttgaaaatgcgatgaagtcaacgaattattcggtaa + atcaccagacatagcttgaaggaaaattttaggtaatactcgagacataccgaaagatcg + ttctgttttagtcatccttctgtcaagtatgtgtagtatcatcggtgccttcaattgtat + aaaggacaaatctttagaagaccaagatattggcctagaactgccggtaaaagtgctccc + aataggcggtttctcgaagtcttgttccacaatcgcctcactattacgctttaattgata + tttatacgtgttattacccattaattttttggttacctgaaacaccatataaccagcaat + accaagacagcaccagtagtcgttcatatctaaaggagttatattcacacaagaccattg + gttagcaagtgcccatgctaactcattcgtagtactgaatgctttatcaataacttctag + agggtacaataatctggtattacaaatgcctaatgctgcaaaatccatatgcttagaagg + tgcggtaggtaaaaacaccatagagtaacaagtgaaaggataagacccaaattctttcga + gtagaaatctataattttttggcagacaactgtcgaatttataactgttaactcatctgt + ttcctgcgtcggaagtgtgaaaatttgaatgggtataatatccgaaccaatgtcgtcatc + catagtatcgttaacattgtccacaacattctctctcaacttgtcttcctctacttcgtc + ctcggcatcaacacttggaggtgatatcaaaggtaaagaccatgaattaaaggcgcctat + agcccaaccaatgtgatgtggagccacaggattaattatctgaaatatgcattttttttt + cgtcaaatcaatcgggtgcggaagttctttaatatttgaatattctgaacaacacacaat + cacatccctcaaactcggattgttgctttcctcaatattccgcctttcctcttccccttc + ctcttcctcttcgtcactttcgccttcctcttcttcttcatcattatcttgtgcatcttt + gcttttactatttttgccttcttcgtcatttttcaagttagaatccttatcttcgtcttt + gataactcttgccggcttcccctcttcctcttcatcgtgctcaggcgtatcctccttctc + tttttcactctcttctccattttgtccgattaatttcgaagtacctatatttttaaccaa + tctcggtacgctgaattctaactcccatgtagatttttcatcaagcaaatcgacacatgg + gacccaatatgatgcagaactgcaaatctcaccatttgaagtgtaaacgttccataacca + gggcttgtcagcatacacagtatcgaatttaatacccgactttgggtttctgatttcata + ttcaatttgtaatgtaattggagtgaaaacagattcttgaaacccaggggtagtcttaat + tgaaggagtaatcggagtgtaattacttaatgcattggcgtcctccaaagatattttgat + ggaagatggtatttttatagttaactgggatttagagtccgtttctgggtattcattcaa + gctagcaaacttagatctcaaaaaatgtgactgttcaatggaattgtcgctgtataatac + attttgtgaagtcaatccattcaaatttgtttgaagtgggtcgtcatgaataaattgatc + acatcgacgattttcgaccagaacatctttaatagtcatttccttgcaatcaaaagttac + atattctaggttttggatcaacggaatgattattatagtagcgctaccggtaatgcagtg + agtagctagatctatatccaacgagattttttcatgggcaactctaaaatttctaaactt + catctcatgcaaagtgctagattcactaacaatggctctaggagtggcgtttttggaaaa + ggacatcatgaagtccacctatttatcctgttgtcacctcttcactgtccaaaacttcat + caaaaaatacttttacatccgttccattttactatagttttcatcttacgcaatttacca + attgccccattcggcaaaagtatttaagtgttacccggttacttaaggtaataccaaaaa + tattcactatattgaaagttctatgtattttattgaattaaataagacttgattttgtag + cacgatatccgcaagaatgattcacaaccattttagatgtctgcagcttttttttttttg + atttttttactaattttagtgcgattttagaataactcttggattaaataaataaaaaaa + atacaattatcactcagattttgaagcaatctgataaaaattgcagcatattattataac + aattctagttcactacaaacagcacattatcaagaaacttatgtttgatggacagtttct + tcaaataattgaatgcagtaaaaaataaaaaaaataaccatggaatatgtttctaagaat + tcgcaatccttgtttatataactatagctttttttatgttataagaaaacatttattcct + ttaaccaaaaggaaaaaggtaacaaccccacttacccctctgtcaatagttaatcatttt + attattttcaactttaaacatccaaaaaaaataaaataaattaaattaaaatcaaataga + aaatgcattattactataataatatctctatgtagacatatacatattatttcattgctt + tttgtaataaacgaggaaaatggtgggataaaaaaatgcattgttattccttcgaaacca + gcagtaataagtcgtcctgagaacgatgtagatttagatctttacatgttggtactctaa + atcacctccttccatcttaaagtcgtccttcttcttgctccaatcaaacttgcttttctt + gctgctgggacaccaatttaaacttattaaccagatagtaaacaaaatcatgaaacaaca + gaaataacaccaaaaatgatcgtaaacgtgaccccttatgatatcacacactgaataatc + atacacttcatcgtctataaccagtccataattgaaataaccgagtccgtatttcaatac + aggtttatatccaccttcatcggtaaacgtacttggactcaaaactgtgcttagatcatt + agttccttggaatgcatgttctctcaataaggaagcatcaagtggagctgggatcatcct + ttgccaaagtacgttaagagcgctttcactgcctactgttgccccaaaaatttcttggcc + ttatcttggcacaaatattcttttgcatttagttctatacatacagaaagagcgaaaaaa + aagtgaaaaataaacagaatgtgataacggtaaaagaaaatcaaaaataaacaaaaaaaa + atacatatataactattattcatgatagtaaacaggtgaattaagatatttagtacaatt + gtctattaccccaataggcataatcctcgaggaaaaagtcgtacaatacccacgagggcc + atattgtgcacagatgccatagcgcatgggcatcaatctgccattcgtaactaaaaaagt + caaacagttccaatgacatggccattgtggttacaatcacaagaagaattggaatgacac + acagtttgaagatttgccgcttgaagctggagtacgcggttttcttgaattcgcccatta + gcttttgcttttgcagagcatggtagttttggcatgataacattatcaataaaatatact + gtaaaacaccgaaaaaaatgttgaatctcatgttgtatgtgtacgaccagtcaacataga + gtctcaagatgtgcagggcaaagattgccgcaactgacgcagtgaacgcttgtgctatct + tggggtacaggaacatggaagtcattcttgcaaatattgcatgaaaccctgttaggacag + ttaaacctgcgaaaaagtaatctagcttctccgttatgatcaaatcacgacagtgaaaga + ccgagcttgctgtccaagccaacattcccgccacagtaacgtaaaggtagttccaaatca + gtatacttctgctgttttttctcctcctatctccctcttcgcgtatgattctagaaaact + ttacaaatcccttataatgtggaataaagttacctatactgaatatcgtcgagaagaatt + cctgagtccccaatactctcaaaaatggccatttcccatggaattggtatatttcttcct + cttcgtcaattctccagcgcgtaatgatgtgctgacattggtaatcacaatctgagatac + aatcccagaacaataacttagagtacaaaggcggggtatcgaaaaactcaatatcatgaa + acatgttggtttcagggtcaatgtagtttatttgggaattcggacatcttctgttatact + cgcacgcatacgtacagtctataaactcatctaaattatctcctggagagcatgtcacta + ggaaacagtgaacaagtagggtcacaaccacagctaacctcatatttcttgccacacttc + tgtttcttttctcccgaagggtatggtttcacaccttaggttgctcttttattcgcttta + tgaaaaatgaaaaatgaaaagtacaagaatacgcaagagaagagcgaaaaaactaatgtt + gacatgacattatgcaataatttatttatgagagaaagagaaaaacactacaaatttttc + ataaaatcacgcctcttcaatcgaatctcaccaagaggtagtgtgacatcgttattccaa + ccccacagttttgaaccagattgaagattctcagacataggagtttgtaaagtggcattg + tactcctccagttccttagcaattgcctctatgttcaatcgagggaacgtttcgtcgtca + tatggatcgtccagtttatcgacgccgttgtaaagaatctggtttggcgtagaaggcttg + aaaaacaacgttcttctatggaaaacattacgcgtggacaaatactcaatctcctgtgct + atcatttcaggctcaattcccttcgagagtaggatcgctgcaactccggtgacaatgggc + gtcgacatagatgtacctgacaaaattaaagtgtcgttgtagttcagatgagatagcgac + tcaatttccacgcctggggcaaagatgtttacacagggcccccaattgctgaacttggca + atcgtgtcaatgtggtcatcaaaggcccctacggtgataacgttttctgcagacgcaggt + gaggcccagtaggcgtctaaattgaagttccccgccgcggcaacaaatacaataccttct + tcaatggccccctccactgccatgttgattatggggctcctgaaactccctagacttaga + ttggccacgcattttttaccctgtgggcgactgacctttgtgcaatgttccacgatgaac + tccagaccactaagaacgttactggcctcgccagacccgtctttgcccaagactttgacc + tccacaagattgaccctctttgccgctccatacgttttggaacctacgagtcccgccacg + tgcgttccgtggccattctggtcgccaaacccttctttggtcaagtcaatcccctggatg + actctgtcttcgaattccggatggtccgcgaagatacccgtatccatgatataggcgttg + acgtcttgaccttgatagtcgtgttcatagtagtaattgaaccagcttttgtagcggtcc + ttgtccccaacgtcgaatgggagttggtagtggcgggaaatccgggccaagtgacggggt + gcgtctggttgatacgttatattttgctcttcctcgacgtcttcgtacgagtatttggcg + gtagcgttaaacgtataactcgactcggcgctatttacactgtcgccttcaaaagcttcg + aacctgaagttgggcacaatatcagccaccagtgggcttttcttcagcttgttcactaag + ttcttggacaggttcatggtcacgccgcggaaggatccgatggagaatttcttctcaatt + ttgttgtcgatgaactgtctccatgacctgttggaattcgaagtgaattgttggaatgct + gtgggatttttgaacctcaccaaatactcctctgcgtatgcgaggttgaccagcaaagag + atgagtatgcactgtggcttcatgaccagtgtttttttgctgtttttcttggcggatgtt + agaggatgtctagcacttgagtatacctctatatgctgtgtaaaatagaaaataaaaaag + ccaatgagaacaatattttgtgtcgttttttttattttcgcttcaattctctatacacgt + acgtcatcactacgggaaaccaaaaaaaaaaaaaagaaaacagtacaaaaaggtttactt + tacccatagttagtatacgtagattttatacacgcactatttttcttatatacaggagat + gggtggccacagaacccgcgcctagcctatttctttcttcttagacctgcctcgaggtcg + ccgacatccaaccttgtacctctgatgtagtagtgtttgtttctttgtctgctcgagacg + tggggggttagcaagtcgattctctcgatcagcggcgaaaacaatggcactctaatctcg + acggccgtcttggctatctggttccgcaacagcaacgaggcgtcttgcaccagttgtttg + cggtctatggaaaggatgtagccaacaaaggtgtcgtacgagcacttggacgagtcgtac + acaatccggaccacgtcgcctgccttcaagcggtccttgttctttttactgatgagcttc + ctcttaacggccccgggatccagagactctatttctgagagggccacctgcttcataatc + tgcgacgaagctatgcgctgcacaggtgggtacacggggatggtttttctcttggtggcg + gggaccatgtaggaccttgtccatgatccaagcaatctgacgttcctgctccacatcgct + atggttctctctccctttcagcagctgctcagagggaagactcgattcttcttcttcttc + tttactgggagttccaaagcaccaaaattgaaaaccaagaaaaaaaggaaaatacatcac + tacataaaagtgcgcggagccattgaccactcacatacagatataagtgtatatatacaa + gtatacatatacaagtatacatgtagaaggagacagagacagagggaaatgtataagtgt + gtatttttatagtatacaaaagaatatgcgtgtgaggatgtgtgtttcttctagaacctg + tgtcttcttttcctcccggtgaacttggagtccttcgcaactttcctcccacgtcttttc + atgagctccttctttcttagaatgaaactcttggcagactccttgtctttgcctcccttc + aagcgctggcgcagttgtttcttcaagttgacgttctcctcgtccatggtcacaccgtcc + aaattcacctgctcctccccctgcggtggggccccactgctcaacacaaggtagtacttc + ttattctttttagactctgggtcgtccaccacaagcccgccactgaaccctgccaccttg + gcagactgcagtatgtcgtccacctggtcgtcgtttttcgggtagaactgggccacaaat + ttccctcccttcttcagtgcagcatacaatgtgttgaaaaacctcatcaaccgctgttta + ggatcgttgtatgaagtgtccgcattgcacagccattggatcgcactgatactaatagcc + gcgtcaaacgagcccgcccggaacggtatcccggtgcccatatcctgcaacatcaagtcg + ccctccagctctctactaagaccggtcgcaagcatgctgggcgatatatccaaaccacac + cacacatggtctccctcctgcgtcaaaatctccccagacagtccggacccgcacccgata + tccagaatgaaactgcacggctgcagattcaaaagctccaacgccctcagcgtcatcttc + gcctggatatgctgcactctggtcgaacccgtgtacttgtgtgcttcgctatcattatag + aaaatctccggtggtgccaactcctcaggacgtgacattatttcttctctgatatatttc + ctgtgtttccgtaccgcacctttttagcactacttttttactatgctcttcttcttctgc + ttcttctgcttttttcctctttatcacactatgtatgtgctgctcatctcttctttttat + cgataaaattgaaaaatgtgagatggtgtagagtgaaaaaaaaaaaaaaatctggcttgg + ccatcaaatacccggccgtggttggactcgtttagcgaacaatagcacccagcagaccct + ggcaacatgcggatgatataagaaggacgagcgtggtggaggaaaggggcgccattggca + cactcacgcaggtggttgttcagcacggcttgcagcaagagcgccaaaacagattgcaag + aatgacggagactaaggatttgttgcaagacgaagagtttcttaagatccgcagactcaa + ttccgcagaagccaacaaacggcattcggtcacgtacgataacgtgatcctgccacagga + gtccatggaggtttcgccacggtcgtctaccacgtcgctggtggagccagtggagtcgac + tgaaggagtggagtcgactgaggcggaacgtgtggcagggaagcaggagcaggaggagga + gtaccctgtggacgcccacatgcaaaagtacctttcacacctgaagagcaagtctcggtc + gaggttccaccgaaaggatgctagcaagtatgtgtcgttttttggggacgtgagttttga + tcctcgccccacgctcctggacagcgccatcaacgtgcccttccagacgactttcaaagg + tccggtgctggagaaacagctcaaaaatttacagttgacaaagaccaagaccaaggccac + ggtgaagactacggtgaagactacggagaaaacggacaaggcagatgcccccccaggaga + aaaactggagtcgaacttttcagggatctacgtgttcgcatggatgttcttgggctggat + agccatcaggtgctgcacagattactatgcgtcgtacggcagtgcatggaataagctgga + aatcgtgcagtacatgacaacggacttgttcacgatcgcaatgttggacttggcaatgtt + cctgtgcactttcttcgtggttttcgtgcactggctggtgaaaaagcggatcatcaactg + gaagtggactgggttcgttgcagtgagcatcttcgagttggctttcatccccgtgacgtt + ccccatttacgtctactactttgatttcaactgggtcacgagaatcttcctgttcctgca + ctccgtggtgtttgttatgaagagccactcgtttgccttttacaacgggtatctttggga + cataaagcaggaactcgagtactcttccaaacagttgcaaaaatacaaggaatctttgtc + cccagagacccgcgagattctgcaaaaaagttgcgacttttgccttttcgaattgaacta + ccagaccaaggataacgacttccccaacaacatcagttgcagcaatttcttcatgttctg + tttgttccccgtcctcgtgtaccagatcaactacccaagaacgtcgcgcatcagatggag + gtatgtgttggagaaggtgtgcgccatcattggcaccatcttcctcatgatggtcacggc + acagttcttcatgcacccggtggccatgcgctgtatccagttccacaacacgcccacctt + cggcggctggatccccgccacgcaagagtggttccacctgctcttcgacatgattccggg + cttcactgttctgtacatgctcacgttttacatgatatgggacgctttattgaattgcgt + ggcggagttgaccaggtttgcggacagatatttctacggcgactggtggaattgcgtttc + gtttgaagagtttagcagaatctggaacgtccccgttcacaaatttttactaagacacgt + gtaccacagctccatgggcgcattgcatttgagcaagagccaagctacattatttacttt + tttcttgagtgccgtgttccacgaaatggccatgttcgccattttcagaagggttagagg + atatctgttcatgttccaactgtcgcagtttgtgtggactgctttgagcaacaccaagtt + tctacgggcaagaccgcagttgtccaacgttgtcttttcgtttggtgtctgttcagggcc + cagtatcattatgacgttgtacctgaccttatgaactgccaccataccacgtgtgtccct + cgcaagcccttgatagatatacaatagggaatgggcgtccgtccaccgtggtcaaagaca + ggggcaaagagctcctaggtctatatatatatctatatacatatttatatatattattag + aactttacaatatagtatataccattcattgtttaagtttcgggtaatactttttttttc + cttgtcataaccccaaaaattttcgatgcctttgatataattgagaacaagaagagtttg + caggtgacaaaaatcgatgattataggtgttgtgacgacaaaatgaacgctaatatatgg + gtggctgcttcagatggtaatttggaccgagtggaacatatcctccgcgagagtaaaggc + gccatgaccccgcaatccaaggacattaacggctacactccaatgcatgctgccgccgca + tacggccacctggatttgctgaagaaaatgtgcaatgagtacaatggagacattaatgtg + ttggacaacgacggcgataccccgttgcaccatgtggaggatgtggccactgccaggttg + atcgtggaagagctgggtggagacttcactatcaggaatgtggagggccaaacgccatac + gactcgttcgtcgagaacggtgaagatggtgagctaatcgagtacatgaggattaagtcc + ggcgtggccgatgttcacggagtggacggcgtgcagggtgagggtgtcatcgacagcaaa + ttgctggaagagttcaaggacaacgtgagatacaccttggaaaatgaccctgaggaagga + gccgatgaggccactctgcaacgcaggaggcagttggaacagatcattacgggagacaac + gctgaggaggagttggaaaggtacatccgtgctatggtcagagagcagatgctgggccag + ggctccatggcgggttccggggacgaaccagattccaagagaagaaaataacgacccagc + acaaaggctcttacagcttgctaaaagaaattgaacgcgacgctacatgaactacttctt + tctcttacatagtcttttcccttatgtatcttttctgtacattaatagacgttcttacaa + ggtaaaatttcaccgcgtttttaaatagaatgaaaaaaacgttgtagagtgaaagaaaag + caacaaatatacagttcacaaggcagcttcgtatagtaatacagcacgaaaaacagctca + tagaaatggtaacacagaccaatccggtccctgttacatatccaacggatgcttatatcc + ccacgtatctgcccgatgataaggtctccaatctggcagatttgaaaaaattgatagaaa + tggattccagactagatttgtatctgacaagaaggaggctggatacgtccatcaatttac + ctacaaacaccaagaccaaggaccatccccccaataaagagatgctgaggatttacgtct + acaacactacggaaagcagccctcgcagcgattctggcaccccagcggactcaggcaaga + ctacatggacactgagaatagaaggtaagcttctgcacgagtccgcaaacggaaagcacc + catttagtgagtttttggaaggtgtcgcggtcgactttaaaagactgaaaccgctgggca + tgggcaagaagaggaaacgcgattcgtcattgagccttcctttgaatctgcaacaacccg + aatacaatgatcaagatagcaccatgggcgataacgacaacggcgaggatgaggacagtg + cagaggcagaatccagggaggaaattgtagacgcactggaatggaactacgatgaaaaca + acgttgtggagtttgatggtatcgacatcaagaggcaaggcaaggataatttgcgatgca + gtataaccatccagttgaggggtgtcgacggtggaaaagtacagtactcgcccaacttag + ctaccttgataggtatgcaaacgggctccgttaatgacgcggtttattcgatctacaagt + acattttgatcaacaatctgtttgttacggaacaaacagaggctcaagatggttccaacg + atgccgaagacagcagtaacgagaataacaataaaaacggtgctggtgacgatgatggcg + tcgagggaagtactccaaaggataagcccgaattgggtgaagtgaagctagattcactct + tacaaaaggtattggatacaaacgccgcgcacctccccttgatgaatgttgtgcaaaccg + tgaacaaactggtatcacccctaccgcccatcatcctagattatacaattgatctttcca + aagataccacctatggtgctaccaccttggatgtagatgtgtcgcacattctccaccagc + ctcaaccccagccaaatttacaaaaagaggaagaaacagatgctgaagacacagcaaaac + tacgtgaaatcacaaagcttgccttgcagttgaactctagtgctcaaaaataccagtttt + tccacgaactgtctttgcatccaagagaaacgctgactcactacttatggtcttccaagc + aaaacgagcttgtgctgcagggcgaccaatacttcaatgaagatgctgcaagaacgagtg + acatatacagtaacaacaacaatgacaggtcactaatgggcaatatctcactactgtact + cccaaggaagactataacatcactgtatgatctcattctttctgcaattattgcacgcct + cctcgtgagtagtgaccgtgcgaacaaaagagtcattacaacgaggaaatagaagaaaat + gaaaaattttcgacaaaatgtatagtcatttctatcaataacgtatacacgtttttttaa + tactatctatattgtgtatcttatatttttccatttggtagtatagcagcaaatatttgt + tcttagacggccaccatccctcacaccaatgcctaacgcttcccaagtttacagatctac + cagatccagctctccaaagacaatctcttttgaagaggctatcattcaaggtctggccac + tgacggtggtcttttcattccaccaactattccacaagtggaccaagccactcttttcaa + tgattggtcaaagctctccttccaagacttagcctttgctatcatgagactatacattgc + ccaagaagagattccagatgctgatctaaaggacttgatcaagagatcttattctacttt + ccgttctgatgaagtcacccccttggtgcaaaacgtcactggtgacaaggagaatttgca + cattttagaattattccacggtcctacctacgctttcaaagacgttgctttacaatttgt + cggtaatctttttgaatacttcttacaaagaaccaacgccaatttacctgaaggcgagaa + aaagcaaatcactgtggtcggtgctacttccggtgacactggttctgcagccatctacgg + tttaagaggcaaaaaggacgtttccgttttcatcttatatccaaccggtagaatttcccc + aattcaagaagaacaaatgaccaccgttccagatgaaaacgtccagactttgtctgttac + cggtactttcgacaactgtcaagatatcgtcaaagctattttcggtgacaaagaattcaa + ctctaaacacaacgtcggtgctgttaactccatcaactgggcaagaatcttggcccaaat + gacctattacttttattcattcttccaagccaccaacggtaaggactccaagaaggtcaa + gttcgttgtgccaagtgggaacttcggtgatatattggccggttattttgccaagaaaat + gggtttgcctattgaaaaactggccatcgctaccaatgaaaacgacattttggacagatt + tttgaaatctggtctatacgaaagatcagacaaggttgctgctactttatccccagcaat + ggatatcttaatctcttctaactttgaaagactactatggtacctagctcgtgaatacct + agctaatggtgatgatttgaaagccggtgaaatcgtcaacaattggttccaggaattgaa + gaccaacggtaagttccaagttgacaaatccatcattgaaggcgcatcaaaggactttac + atcagaaagagtttccaatgaagaaacatctgaaacaatcaagaagatctacgaatcatc + tgtaaatccaaaacattacatcttagatcctcacacagctgtcggtgtttgcgccacaga + aagattgattgcaaaagataatgacaagtccatccaatacatttctctatctaccgctca + cccagctaaatttgccgatgctgtaaacaatgcattgtctggattttccaattattcatt + tgaaaaggatgttttgcctgaggaattgaagaaactatccacattaaagaagaaattaaa + attcatcgaaagagctgacgttgaattggtcaaaaacgctattgaagaagaacttgctaa + aatgaaattataagatcttcctttttaatgaatatattttatcttatataacatatagaa + ttctataattaatagagagtatgaagtacgattatccttaatgttaatcaaagtgaatca + tgattgcgagagtaaggaaccataaaattaatcttcatctatgctaataatctcagaatt + ctcgttcccagggtcttcttctttagatactaatctaactttcccgcccttttcaactga + tatttcaaacccagctttgctcaatgcatcgtcttgaacaacgtcttgagcttccatttt + tttgacatattcctgattcttggcattattctttaacaaaaacttcaagcaaacaataga + tctttccttgataaacggatcattatcatcgatgacacaattggaaagaattaatgcaag + accacctaattctcttatttgatcttggatttcaggatgtgcatacactaacgatgccaa + tatttcgattattactaatttacaatggggaaaattatcagccctaataaaggattcgtt + ggtattaacacggttgaaaattaataaccggtcctcgattttgttaccttcatgatctgt + gattttaatttcattcaccgtatcaccgtttcctttcaaaaaattaatcctcaacaaatt + cttttggaaagtatccaagagagataccaatattttcacagagtcatacgaaattaagaa + ttttctaacatgattgaatttgcacaagtcagaaattatgtccaggcaagaaagtaaagt + ggagtgtaattggatggcaactgaaggagcataatcgttacaacttttattgaaaaaggc + aggaatatccttgcatgcaagacgttgcagaatggggtagcaccatgacataattgcagt + caactcataggtattccaatcttctttactcgttacaaccaattgcaatatctgcaacaa + agtggtgaattttcctggctgtctttcttctaacccattgaggtatttaccaaaagaagc + agttgtaagacttttgctaatacattctaagacagataggtcaatagaagttatttccaa + ttttgaatcatccagatagtttctgcccttctcaattaaaaaggaattctcttgatcctg + gatgaatttggctgtcactatatcgaaaaatattctatccctaacacaataatagtcttt + ctggggtgtatcattatccctgaaaaattcgttggaaaattcatcattgttgagatacgt + cttgaaaagaaataaatatggtagccagaaatgctcttcttcttcaccgtcttccaattt + gatcccataagggtattgaagaaattgtaaaagagctggaaaagtagtcatatccactgt + agagtagttctgctgtatattgaaaaggcacgttaaacatgcgatgtatagatgtttgat + tagcggagtgtcaacgacatactcactagttgcatgcagaaaaccacgaattacgaggtt + ttgtagtaaaagcttttggggtatttcctggttttcagcggcaaggtttctcatcattaa + gataaggcccctcactgttcttgtatacggtattgagctattctcatttaacgacgctgg + caacagcattgcttccaaggtgtctcttaatcgttcccaaaaaagctgggagttagccat + tgatttcctatgttcttcatcattaaacgtcctttgaaatatacggttcaggttttcgac + gattggctcataatcctcgacgcaacatgggtcgttcttcatcatggtaatgattgaatc + aaataaattgaattcatctagaaaattgttcataggcattgttatttagttatcttcttt + tttgatgaatcaataattttataaggcttagtgtaattgttctcccgatgatagtatgaa + acggtagtcgataaatttgtgtcaaattttttcctcatcccatcaattcttcattttttc + ccttcctatttttttttttactgaagcgatgatgccttatatataaggaaaatagcctcg + tcagtcaaaggacgactcccgtaatggtagcctcagtttatattaggcgctctcatcatg + atgtatatcgatattggcgatgatatttttttttgttgatattaaatacaaatgtaatgc + tttatatagttctatacttggggaatacatgatgtgaatgtgcataaatagaggacagtg + aatatttttcaaggaagctctttctcattttcttcttcatcactggaatcattttcatta + gacaagggtaatttgttatccttcccattgaaaccaatccatccttcctcgtcgtcagat + tccatgactacatcgttctcttcgttctcttcgttatcttcatctgcgtcatcttttaat + agaacctcgtcatcatcagccgcgccatcttccatggacccatcagttgataccaaaaat + ctataggtgtatttgttatcagtagtggtattgactacttccttagccactctaacaata + aatctttgtatcgacctcatagccgtcgagaagagatatttgtgttcatttatgtaacca + ccgctcgcagataatagagctttgatccaaattaggttaaactcaatgtgttgggattca + atggcaaaatcaccgatgaacttcagaatcctcggtaaatatattgcaggaatattactt + gcaaccaaggggatttccttaataggtatggcttcatagactttattgatcaaatattct + tcatttaacctgaacgccattactaatgcatttaaaaactgcttttctcgtagcgcctct + acagtagaatggggggtgacgtccacatccagatcaaagggatcaaataatatcgtgtca + ttggtggaatatatcaataaaccttccgttgaagcagcggcaaatgcattcgccgttggg + gagaattgcaccgaagtaactctaacctctggtctcatttttcttgtggacaggtcgcca + cctctttgagaccctggtaaagaattatcaatacgatcctccaaatctgaattttcgcct + gcatcgtcaatcaaatctaatgaacctgcttcagtcattttcttactgtttaaaaattcg + agagtaccattcaaagccatgtttctggacacaatgaatctttttaacaagacttcattt + ggaacatcatatagacaaatggagttattattaccaccagccacaatagccataccatca + aaactgtaatgtattgtagtaaaaaatttggatcgttcagaattttggcagtgaacctat + cttgattaaacctaccagatattatatcctttctacagtcaatgttgcccacctgcttgg + catcttctatgttgaaaatggatatttgaccctttaaggtagatactgcaacttctttac + catctggtctcattgataaggctaaaacatcggaataaacttctataggttctacttgtt + ggcttctaccaaatatggaccagattctaattgttttatcccatgatgcagaagctaaga + cactgttctcttgactaaatgaaagacacgaaacagggccttcatgtccggacaaagcat + ctaataattgaccagtttgcacggaccaaacatgaatgtcaaaattgtccagggacccgg + cacaaaccacttcacctgatggatccaccgctaaacaattgaattggattctttcagtac + cagtgaatgttctaaaattacgatacctgattaagtcccacgctctcaccgtaccatcta + acgatgatgagaacatgacctgaccccttttcgcaaactgtacagcagtaactgaagagg + tgtgttcttcaaaagtggccaaacaaaaccctgatgtaatgtcccaaactttgattttcc + catcttcggatgctgtcactacacgtgaaccatccggagagtatgcaagactatttgtgg + aatcgaaatggccctgctgcttcaagatatacgattccgattgccattcgtaaactagta + attggcccagtttgctggaaccaaacgccagccattcgccggtttggttgacgctaacgg + tgttgactgggttttgccccatagaaagctgttgaatcaaagtgaaatcaggcaaatcgt + aaagacggaattccccactagtaaatccgacagctaaaagccttgttgctggatggaagg + tgacacactttactttggcttggtttgcgtaaaaaaaatgtttctttgtgattctccagc + tgtatttcgaaatatctacttcttcttgcttgtcgtcgtcttcactttcattgtcgtcgt + catcggatggcctcttggtaaattcccagacaaagacagcaccgtctttgcttacagtgt + agattttttcctgatcatgactgaagaacgcacccataacgtagtctctgtgcccattaa + atgttgtcgccgcaagttcttttcctctgaatctacggaccatatttttgcgcttaagtc + tttggaagtcgtaaggataaatctggaatcttgtgaccacgtcaaagacgttatgtcttg + aaagtgtcccgcatgcaccctatggcggacgaagggagcaaactgtctgtctttattaac + atctggagtcttccaaatctgtaaaaacctgcctgaggctaatgcaaagagtctcccatc + agggctgaacttcacagcggagatttttctttgaagttgaaatggtgaagcacgttacgg + gctttgaaattgacaaggatggcgcgaccgtcctcgtcaatagaaatcagcaatgtgcct + tgtttgttcagatcaatggcagcaatatttttgcgatgctcgtattcaaacgtgaacgat + ttgttgttgattaagtcaaacacgctgaccctattccccaccggtgagagtagttgcttg + ccatcatcggaaaaggtgatgttaccttgcctgtagaccgtacctaaaaggttagagaac + ttgaaatcggatttcattgggatgcggttcaatctgcggtctgctctcctgtctacctgt + tgaaaacgatactattattactattattgatattatactagttattgtacatacctagaa + ctatactcgaaactttttttttttcatctttcagctcatcgcaaaatttttccaaaaaat + ttcaaaaagaactcccgtcgcatgtgatcaaggttacaggtgcttgacataatcataatg + attacgagtcgaagccggccctgacaacagcttctcttgccgtagaattgatgtgtttaa + accggtcgggacctatgtgggcaccgccgaaccaacgggccaccacaacgatgacgttcc + acacatccatgatggtgatgaggtgcagcattctggagccggcggccgtttcaccgtcat + catcggaatcttgatatgttgccgcagagccatcctgcttgattcgccatgcactcatga + catggtttgccttacgcatcttggagtcggtcttcagtaggtctagcatggcaaacgctt + gttcctcggaggtaacatgtgctgcaaaggccatgaaagtcgagcctctatcagtaatgg + ggtccgacgcggtccagccctcgaaggggtctgtgggaatgtcactctgctggaccggtt + ctgtctcctcctctggttcaacgtacaagacaccgtcgagttctgtgaggaagtcaaata + gacagacagatccgcggtggaaaacagagtccatcacttcctggaacaaatgctgaaggt + acttggtatcgtagagatcgcgcttagccaaagaagtgcagacaccaacttcgatgacat + taggagcctcctcggaggggtagtgtgtcgggaaggagatctgcagtgtcatgtattcat + gctgcggcactttcacaacgatgatgcttccgtcttcctgcttcttggagagaagatccg + gatagatggcctcgacggcctccagttcttcgaccaactgttcgtgatcgtcatccattg + agcttttctttcctctctcttttttttttcttgttacatattcctatatatatgtatata + tatctatatatatatatatcccagcgtatatgactgcacaagacgcaattttcttgaacg + gttttttcttttcagcactgcgaaagagagagcaacggtaaagagcatgagccaatttga + aaagcagaaggaacagggcaattctttgttcaaacagggcctgtatcgcgaggctgtgca + ctgttatgaccaactaattactgctcaaccgcagaacccggtcgggtacagcaacaaagc + catggcgctgatcaaactgggtgaatatacacaggctattcaaatgtgccagcaaggact + gcggtacacctcaacggcagagcatgtagctatcagatccaaattgcaatatcgtctaga + gctggcacagggagcggtaggttcagtacagatccctgttgtagaggttgatgaactacc + ggagggatacgaccggtcctgagaaaagagaaagggaacacttctaatttatctttaaaa + ttatatatatatatatatatatatgtgtgtttgtatactctgtgggtatttccgtgtata + tggttaataatagtagtatcttgtcagttttttttatgtttttcttcgcgcgtcaacttt + ctaccaagagaaaaacaatataaggtctccttactctataggagaataaaacaaacaaaa + ataaaaagcacatcgtagcgccaagaaaatactgcaaataccaaataccacaataatact + acaattatctatacacaagtgttttgccgcttaaaacttcgattttcatagtacgaaact + atacacccttggtttttctcttttctaaatacatatctaccttgtaaagaatttcccgca + catctttgcgggcatacagttcatgtattggcaactaacggaactaaggcaacatatctt + gcatattgcaatgttcactacatagatgaaaacttatatctaggtttcacgacacgagaa + taactaagaagcacgatccatgatatagaaaaatcagttacgacgaagcacggcaaatta + gccgccgaagaccgatatttttgccaccggtcacagttttcttttccacggagctcttcg + cggtttttttgttccggattttttttaccggctcttagcggaccgttttggttataaggg + gagtggcagcggcggtagacactgcgctctataagaatacttgcaagggtcttgtctatt + gtataattcgctagtatttgttttgcattgtactcttaataccccaaccaaaaacaaaat + agtgagagtaatggtgcgttttgtttcaattttaagtttattcggctgcgcggcgacgct + tgtcacggcccatgatgacatggacatggacatggatatggacatggatatggacatgaa + tatcgatacgacaacgtctcaatccatagatgtctcatccacggcttcaatcgtccccgt + gccacatgaaccaaaacatttgcatggccttcctatactgcaatcgccctcgcttacccc + tgcggagagattgtactgggaaaactacaacaccacaacctactttactacacaggctgg + gaataggtctgcccttcgctaccacattattacgctgctcttggttgcatttgtgctcta + ccctgtgtccctggcgctaagcgccgcccgttctaggtggtacttacccctgctgtttgt + taatctatgcatttgtatttcgtccgtaatggcattgtccgtgttcaaaaatactttccc + ggaagaagactggtatgcgcataatatctatggcaccacttctgtgctacttctcgtttt + tatgcttgttcacttcttcgctgcggtgctttctgtccccgtctcattagcatcgaaaaa + ggagtaccgtccggttgacaccatccctctgaatgatcttgaatctacgcccgtcatggt + gaatagtgcacgtggctctccaagtccttcttccaacagagacacgttgttctcgctctc + ttcagacaccacgaccgccacggccaccaataataataaacggagacgcgctgaaggcga + agacgagggtgataacacctccaaccacgacactttgcgcgacgaagactacgataatga + tgacgacgaaattgcttccattgaagcgccacctctgcttcctcaagacatacccgtttt + ccgaatcttgtttaccaacacgaagtaccagatgcttgccgcgcacctctcgtgcgtcgc + caacgtggtctttcacatgcttacctacccgctattcatgtacatctttgtagacctaat + catcggcttcgctgtaggtaacttgctcggcaagggcatccgcatctttaatctcttggc + ccactggattaagggcggcgtattttttactctgggcgttgtctctttagcaagatactg + cggtttcgcagctaagtacggctgggcatggaacaacatcagcttcacctctcaactcac + acaaacgcgttcctccaatcttcttttccggtttgctcctgcggggactttcaccatgga + attcgttgaatccttcctcattttcttttacgggtccaccaacatcttcttggagcacct + ggcaggaaacggcggcgcatggactgccaaggatttacagcatgtgtcgataaattctca + ccggccccaaggtgtgtgggctactcacggagtacaagctcaaccattggcgattcgagc + atgcccgcaaacggccacagaccgatgtagttgctgccacaccggggtactctccaaacc + cgttccccgctttcaccatattttggactgggattctgatgtcccagcacgcacagtcct + cgcaattttctactaccattcacacgcaatggggatacttgttgtcctatgggtccttct + tccgtctgctaacatttttgattctgtttttggtgcccaacaccaacagtgccgcatcca + agcctttcacggagttgatcacctcgttctgtctcctctgtggtggtctggtatttatgg + agtccacggatcagtccattgaagccatggaatatagggggtttacccccatgttcactt + tcaacctcagtgttggattcgtttctttgttgatggcttgggaaatgattttatttattt + ggaaagactggctcatcaaaaccaggaaaaccagtctttaaccgccataatggcttttct + tttttcttttttttttcacccccccctcatctttgagtgagaagcttatatgctttactt + ttcctcacctactttttaattcttaattattagtttatccagcattcagtcaattctgaa + tgcgttatcgcttcctcaaaatttataaaaatgaataagcaagatctcattggaattgag + ttcaatgacgattattgagggcccatacctgataaaagtgaatatattatgtataatgac + tctactggaggttctctctgagaatgtaaaataatccacaaaagacatgaaaattagtta + ctctacataatgtataataagttcttccttccttttttctttttttagtttttctactca + ccatcctacctacgccaagctgctctactgtcttggatctgtgaaaaaacgcctagaaac + atgtataaaacctaccgctttaaaccataatttcaaatcgaaatggcactatggccgagt + ggttaaggcgagagactcgaatggaataaaaagttcggctatctcttgggctctgcccgc + gctggttcaaatcctgctggtgtcgtttaattttttttaaataacatcgttgattaaaac + aatcctattcactgcagtcacagacttagcacatttacaaccagtacaagatcaatcaca + cgcgttgtggtaggaactttgacgatgatactgcgttggctcttgaacgcatagtacatt + tgtcgttcacaacggaaagtgaaatactgaaaaaaaaagaaaaattttcttagggactca + tcatcaatgtaaacacctctacagccgtcaagactagcaaagctcacctcacaaaacgac + atgccgcgcataaagaccagaagatccaagcctgcacctgacgggttcgaaaaaatcaag + ccaaccctcacagatttcgaaatccaactcagagatgcccaaaaggacaagtcgtctaag + ctcgcagcaaagtccaatgagcagctctgggagataatgcaactccaccaccagcgctct + agatacatatatactctgtactacaagagaaaggccatctccaaagacctttacgattgg + ttgataaaggaaaagtatgctgataaattgctaattgccaaatggcgcaaaaccgggtat + gaaaaactgtgctgtctgcgctgcattcaaaagaacgaaactaacaacggtagcacttgc + atctgcagggtgcctcgtgcacagttagaggaagaagcacgcaaaaagggcacacaggtg + tccttccatcagtgcgtccactgcggctgccgtggatgtgcaagcacagactaacgacta + tatgcacgttgcagtattgttctggccatgtaattatctctctcccaacgttttagaaga + ggttttcgtctccttcgtcaaagacgaccactttcccattttggtcattggaacgcgaaa + aaacaaaacagacgcgagaacgcgacgcgaaaaaatatatttttccgttttttttttttt + tcggaacaaagataacgcgttaaaacctgtggatgtaataaggatctgaaacactgacta + aaacgcgtcctcatttaaaatcaaaaaattacttcttctcctccttacggtgccctttga + tccttctcaaactttaaatatatgttgcatgattaattgaaaatcatatagagtatatct + taacagaggttccaacgctttaaatcgtattctattcttttccccccttttattatagtt + acatctactatttgagcttcttttattgaccgaaactacctcttatttcgaagcaaaaaa + aaaaaaaatcaacaaaaattccaacttttcaatgatgaatgaagacatatccatcattga + tggccataatagttttttaacggaaaaaagcaccgtgctattaacccaagccaagagaac + actagaagacgaaaaggaaatgattactcccccgagctcaactgtgagaaaaacaatgaa + ggaagtaaataagaggccgtcgcatcccctctcaccggatcactcgtccccaattgctcc + atctaaggccaagcgccaaagatcggacacatgcgctcggtccaatggtaacctaacctt + ggaagaaattcttcaatctttggaaagaagaagaataaatggtgaactcgccaagaaacc + tccatattcgtatgcaactttgatttgcttggccattttgcaatctcaggagggaaagct + aacgctatcccagatatatcattggatccacgttcacttcccttattacaagcagaaaga + tgctagttggcaaaattcaataagacataacttgtctttaaatgatgcgttcatcaagac + tgaaaagtcctgcgatggtaagggtcatttctgggaggtcagaccgggtgccgaaacaaa + atttttcaaaggtgaaaatcgtggttatgaatttgtaaaggactccttacaagacattgg + gaagtattttgaaatagattctacacttgatgaattagaacaagttgagagtggagaagg + caatgatgatcttcctgacgaggaagaaagagaggaagcagggaaattcccttccattga + aattcaattgaactcctcccctatactgagagtttcccagttacatcacataccgcaatt + gaaaacagacaacagtgtactgaaccctcacgaaaacctagaatcgatgcggaacatgat + agaaaacgatgtcaacaatatagattccttggaacctccttatgtcatgaagaaatatca + tacttctttaggcttaccgtcgctggtgaatgccaaagatcatttccaggcgggtgtgaa + aaacaataatatcacccaggcaaatagatttaatacactccctataactagcgcaaagtc + tcctcagaatttcagaaaatatttcacctcattcaattcaaattttgaagatttatctcc + acttcgaagtaatgtaggggctggttctctactcgacccacttccgtattccccattgaa + gctgtacgatcagaaaaatcttgcgctcatgtcgaaaccacaatctcagcaatcatattc + caattctcaacttccacctccaccttcctctcatggttcggacttacttaaaacacccaa + gatgaggcattccgatggcttagagaaaaccccatcgcggttgataagcacacctaagga + cggtaactcgattttgaggaaatggcagactccttcacacctttttgaagatttgtactg + ttctccgctatttagagctatagagactccaatcaggtatatcacgacgccggggggcaa + ctttggaaacccaaatttcaccaagaaagtcctctgcacccgatgtcctcacaagcgcaa + cgaattccaaatttgcttcaagcgggctgtttggcgtggatgtttattctgtttggaagc + gcgcaactgaaaagatttctgatggtaacaatactacggatagcaatcaaaaacatcatc + cttatcataatcacccttccaacgatagcggtaatgaaaagaattgactttttttttttt + ttttttttcattttcttttcctcaccgaaaactaattattcttaatctgatggattcatc + gcaaacggttggatgagtcctaaactgggctggagttatcatatcacatagcattttctt + tttaatcttattgtattgtcgggtcaactaacttcttttcccgctcttctatggtataat + caattgcagaagaggcaggaaaaaattgtaatcataacaaaaatgatacaaatttttttt + ctaagttttatattttacatatctttattttttgatccttacggatttttaatacagtat + ttagctgaagtttccattcacttcacttacctttttgatctcttttatttttacttgccc + cgttgccttgcatttttcaatagaaagtgaaaaactaagtaccacttgagcaatgccaca + ttagaagaaaaaccatccgcaagtgagcatcacagctactaagaaaaggccatttttact + actcatggaccaccaaataaccactgcaagcgacttcacgactacttcaataccgagcct + gtaccaattggatacacttttgagatgtcacatttgtaaagattttctaaaagtccccgt + cttaacaccttgtggccatacattttgttccctttgtattagaacacatttgaataacca + accaaattgtcctctctgccttttcgagttcagagagtccttgctgagaagtgagttcct + ggtcagtgaaataattcaaagttatacatccctacgatcttccttactagatgcactaag + gataccgaagcctacccctgtccctgagaatgaggaagtaccaggtcctgaaaattcttc + atggatagaactcatatcagagtctgaaagtgacagtgtaaatgccgctgatgatgactt + gcaaattgttgcaacaagtgaaagaaaacttgccaaaagatccatgactgatatattacc + actgagttccaaaccatccaaaaggaattttgcaatgttcagaagtgaacgtatcaagaa + aaaatcaaagccaaatgaacaaatggcccagtgccccatatgtcaacaattttatcctct + taaagcccttgaaaaaacacatttggatgaatgcctaactttacaatcactaggcaaaaa + accaaaaatttctaccactttccctacagagtcaaatccacataacaaaagttcatccag + attcaaggtacgaactccagaagtcgacaaaagctcatgtggtgagacctcacatgtgga + taagtatttaaactcaatgatgagtgcagaacaccaaagattgccgaagatcaattttac + gtctatgactcaatcccaaataaaacaaaaactgtcatcgttgggactgtcaactaatgg + tactaggcaaaacatgattaaaagatacaatcactacgaaatgctttggaattctaattt + ttgtgattctctagaacctgttgatgaagctgaactaaaaagacagttgttaagctggga + tgtttcacacaataaaaccccccaaaatagtagcaacaagggtggaatttctaaattaat + gataatgaagagtaatgggaaatcttcttcatataggaaattacttgaaaatttcaaaaa + cgataaatttaataggaaaggatggatggttatgtttcggaaggattttgctaggcttat + cagggaagcaaaaatgaaaataaaaacaggttcatcggacagttcaggttcagtgggaca + ttctaatgatggagatggtgttgaaaaagttcaaagtgaccagggaaccgaggatcagca + aatggagaaggatcaggacactgttatcaacgaagatagagttgctggtgaaagaaattt + gcctaacgaagattcaactgatgctgacttatcaagagaattaatggacttgaatgaata + tagtaaagacccacccggtaacaattaaggtgtgtatatgtaatcaggcctgtttgttag + cttgtgcacatttgttaattaataatttatttaaaaatgggcaaaatataaatgcataaa + agatgacatagagatatgtgcacaaagctatgtaatcatatttaacattaatatatatta + tttcgttattcacaattcgtcatgaaggccagcgtagtttacggttcctgctactgcatt + atcatcgttgacaatgtttgtattcgaattattattcaaaggtgctgcaattgtttgcgg + aggggccactggattaggcaaagaagtgatctccacgttaagtgctgaaataacttcact + agatagagagacgtcttcggtaacatctaccgtaccagatgcctgcatttccgtcgtaag + cattcgggaaacaggagtatttacattttcgctgattttgtggcgtaccccaaacgcttc + agatgaatcttttgatgactgtacttctatcattatttctttctgtacaaatcctgatgc + atcaattgaactagcagatattttgtgagggctacttacaaaagatggtgctgttgtggt + tacacctggcatcggctgagctactgtgcttgacgcgctctccacggataaagttccctt + gcctatatcagacagagcgccaattgaagcaccactactactaaagatcatagatgccat + agaagtcccttcaatatttgtggattctgatagggaagattgaccaacactcgtcttagg + tgattgtacagcatcagtgaaaacactagtgggttccgaactgtcagccaaaacactagt + gggttccgaattgtcagccaaaacactagtgggttccaaattgtcagccaacacactggt + gtattccgaattatcaatcaaggcactggagcgttctgaatcctcggtagagaaagcaat + tactctcgaagtcttcgtttctgtttttgagaatgagctagttgccgtctccacaatagc + tgatgaaacaggttcagagatggaatgaaatttagaaaaagtggaatctgagattgaagg + gattgattcagaagcaagaacagaagaagttacagtcgatacttctattgactggaagcc + agaggttatagatgaatcagaaaatgtactcggatgcgaggaaatagagtcctgggatgc + tgatgttgacaaaatttcttcatttgggatcaaattagacgcaactgagtcaaattcgga + tgctatagaaatagaatgcgtaggtgtttcaaccatacgctctaattctgatgctatggg + atcaatttcggagacgggcgaagaaaccatagtgtctgtgggcgtcaacaacattgaaga + agatgttgtaacatactgctcggcttctgcattggaatctgatgcaggtatattcctata + ctcaatttttgtaatgacttcagtgattatttttgtcttataaatctctctcgtggatgc + cggttttgagttgtctagaaaagatgtgttggctgcatcattggtggggtttgtttgtgt + ggtggtaaccgcagtcgcggtacttgtggataatgctgtagtagggctagttgttaacga + tgaggaaagagataatgaagatgaagatgaagacggcatgtgtgatagcgaaggtgattc + agtacttaagagttttgaaacgctttctctatctgttgttatttcgctttctgattgttc + tactattaacgaggacaatttctcctccaagtcaggagtcggctcaggatattctgtgat + agaatttgaagatgaggataacaaatctatcgactctgaattcagtggaagcgatggttt + ttcgacaatattcggttctgtaggagaggctagcggcttcgtaatttcagaggtaggtga + agacacaattggttcattagtgacactctcttcatagaaagaagaagaagaagaagaaga + agaagaagaagctgatgttgggatatctgcagaagtgaattgtgaatcagctgatgacga + tatccaaacaaatttttgctcatcttctgtggtggttctcatagtgctagtgtcaaatga + ggaaaattcagtctctatatcgctagcaacatctgtagcgctgatgatatctgcactttc + agtaatgcctctgtaatcgtcattaatagtgaaataagaagaagatccttggtctatggt + agtgttttccttgaatttcgacttatatatatcatgagcatgtttgtaaataaagccgtg + cacattatgtttgtagcctagttgaatagcgccagccaaaaccactaacaaaacaaaatt + caagaaaaatttccaaatatttcgtaaaattgatgacgatgtaccggaaagcttcagaac + gttgatcgtgttccccatagaagtgcttgccaaatatctagaatctggagaaaaagtaac + ttttgttatagtagattcatgtactcttggaaccaactgaacaagctttagcttttcaac + gctgacgatggcaaccaagttatcgttagatgataatgcaacgaacttcttatttgggga + gacatccatcgatgttattccattcaaatcgaacatgaaggatgctgtttttaaaatttt + aacacctttggaactcacacctagttttaacaaggaaactccatcagagttcgataatga + tgctgccactaatagcgtattgtcattgagaaacctgatattagaaagaacaagattttt + atcaaaatccgttttccttgctacaaaatttccgtccctaacagacgctacttccaaaga + attagaagtgatatatgacaaaagaataccgttaggggcgaagtgcaaatcattaacttc + cctacccgtttcaatttcgtaattttccgtcaaattacgcgggtcaatgattcttatcac + agtgggcacttcagaggacgcaattgcagctactgtggccctttgattaatgtaaacaaa + cttagtgaaaacggatgggtctgtagttctgttgaaatcaatactggccacgaattttag + atgttcttgttcaaacacaaacttcctcaagtggtggttaacgttctccatggaggaatc + attaaacagttcattgcaaccaactagaatcaggttattattacaatccaatgaagtggg + cgcatcgtcaatcgaatctaatgtgatctcacgaaaccttctaatgtgtttctttttctg + gaagttcacctttaaagctgtaattttgtttggaaacgaggaattgaactggcctccacc + accagccaccagtagagtgccttcatttataaatttagcaccatatatcggatatccgac + gtcatagtttgcagagttgccactcattattatgctagtttttctaagtttaaaaggaga + gaaaaaaaaggaagagaggacgtatcgtagggaattgtgatgcttgttgacaaacagcct + ccgtcgatcgaagcagctataagcgtaggttacttgaagcgttcacagttctcatttaca + ctgtacaattctcgagatataaatgaaaattactacaaccaagaaaaaaggacaggaggg + ctaaaaggataataaaatagggcagttacaaatgtggtcaaaaatactagggctcaaaat + tgctaagtaagggcttgacagatgtaacataatacgaaataaactggatatcgtttaata + attatcaaggtgatttaaatcgtaaatacataggctgggcatatacactaacatgtgtcg + tgaccaatgtgcagcagatagacttgctcattaaatatattccaggtaggattctctaag + ggttttttttttttctctatagctgtcacttttttttttttctcttatcaatcacagata + taataagcataacatcggaaagaaaataatgatagggcaaaagggaaagcatttccagtt + gaatacagtatctctactatattgttgttcgctgaagacagccgtttccggaaaaaagtg + attgtgtgatcgcagttacccgcacctttgttcaacagtacataataagaatctgtagat + atagatgaactttctctattacataagactagcctaaaagccttgtttctatcatttaaa + ggcttttgagacactcaaatcagtgtcaaccttttggataagtcaaagaaagtacgccgc + tgctcagaaaattgttatcgtgattgagaactgatctaggcattacaattaaaggaaaca + agggaaatattctattgaatgttgcataaaagcccttcaagaaagagatttgcttctcct + ttgcatctaggatgcattctaacgcttacagtgctctgccttattgcttactactttgct + ttaccggattatttatcggtagggaagagctcatccaggggtgccatggatcaaaaatct + gatggaacctttagacttaagtctatctacaggcatggcgtcggagcaaaccatcggcta + caccagaggctggaggtcactccagaagttatttctgccgctggaatgctgtatcaagaa + accacaactcaaggacaggatttcgaagaccaagaacccctatggactactaatgccgaa + tatgccaccactaacccattcgattttgagtttgagctacgaaggatgccgctattgatg + aagcggatgaaggaaagagatcctgaattcatagagtcgtacatttacggagaaacatac + atgacggaggaggaagaacacgccatgtggatagatgatgacattgtggcacccaatatc + acagatagaggtacagtagtgtcgttagcattaatgtcgtctaatgcttacgtgagaata + ccgcaaacgggagactggcgtaacgtcacggagccgtggaatgaaacagagccggaagat + tttggttgggatggtgatggtatacgtggtcacgtcttttacaatgaagtggagaatatc + gtggtactctcgataaagggaaccagcgctcagggtttgccagggtctggcgaagatgag + acaactggaaacgacaagatcaacgataatttgttattttcgtgttgttgtgcaagagtg + agctacctgtggactaccgtgtgcgattgctatgtgaagtcgtatatatgcgatgagtct + tgcctggaaaaggaactgagacgtaaggatagattttactctgcagtcgttgacatatac + aaaggcgtcctcaaagaatacccgatgcggccatatgggtcacaggccactcactgggag + gcgcattggccagtttactgggccgcacttttggattgcctgcggtcgcattcgagtccc + ctggagagctactaccttcaaaaagacttcacttaccattcccaccgggacttccctcat + acatggagggtatctggcacttcggtcacaacgcagacccaatcttcatgggtacatgca + acggagctagttcaagttgctcactggtgggctacgctatggaaaccgcgtgccacactg + gtagagtctgtgtctacgatgtggtcaacgacaagggctggagtgtaaacatgttcaacc + acagaatccacaaagtcattgacgaagttctccttggataccgagcaggctgccaagtgc + gttgaaccagagccctgcgtagattgctacaactggaagtttattccaagcagagactgg + gaatcctcatcgaggctcattaccaagactaaaagccacgctgcaccaaccacgacgact + cgcaccactgctactactacctcttcatctacctgtgtaggccgcaattggcttggcttc + tgcaccaaatacgagttgtaagacccatacatctattatgaccctagtgttgttttaggg + cctatgcgccctttttttcttccattgtctgataaataatgtatcattttgacaacttaa + aggttcagctaaacgacacgagaagatatattaatactgctttaaagtgaaaggcgtcag + tcaggtgcaattaataggtcacatattctttgcttcaattgctctctagcaaactcacac + actcgtagattggaagaccgaagaaaatagatagactttcgaataagttcagcaacaaca + tgtggttgaaatccttgctgctctgcctgtactccttagtactctgccaagtccacgctg + caccttcatcagggaagcagattacctccaaggatgttgatcttcagaaaaaatatgagc + ccagtccccccgccacacatcgtggaataatcactatcgaatactttgatcccgtttcga + agtcgatgaaagaggcggatctgacttttgagttgtacggtactgtcgtgcccaaaactg + tgaacaactttgctatgctggcccatggtgttaaggcagttatcgaagggaaagatccca + atgatatacatacttactcgtaccgtaagaccaaaatcaacaaggtttaccctaacaagt + atatccagggtggtgtggttgccccagatgtgggtcctttcaccgtctatgggcccaaat + ttgatgacgaaaacttttacttaaaacatgacaggcctgaaagactcgcaatggcctatt + ttggacctgattctaacacctcggaattctaacacctcggaattcatcatcaccactaaa + gccgatggaaatgaggaattggatggcaaaagtgtcgtgtttggtcaaataacttctggt + ctagatcaactaatggatgctattcaatacacagaaacagacgaatatggaaagcctcag + catgaattacggttcctgtatttcgttctagaaatcttaaaaattagtaacatcttagat + ttgcacgctgcgtacacagaaaaagtcgagaagtttagaaatggcgatgtgtctgttggc + tccactttggaaaacatcttccgtaacgataaagcctacacacctttaaccacctccact + ggaaccaccgcctatgatttaaaccacccaatttccagagccttgatgtgtttaactgtt + cttggcctttgtttcattgcctacaagggcatgcacgaaaagcctcatacggtttcatta + agacacaagtaagcaacttcttttcgatggcttgatgaccacatggctatgattttaatt + aatatatatacatgacaattatttttttatacattagcgatacctaaaatttcttggtta + atacccttttgacagcctctactgcattgcccttgataataatttttttcgattgcatca + ccacagccatgattttttgggtatgaaaggcagttgctcctgcaagtcatttcttagttg + aattacatctccttcaatctttctgatctccgtgataatcttgttacctccatttttata + agcggaatacaccggtagatttccggtcctgcttctctccacgtagtaggtctttttacc + aaaattgttgtttcctatgagctcatgcatcttcacgtcttccagctttggaaatattgg + cgcctcagcagctttatttgaattagtggatatacatcttatcgtttggtatatcgaggc + agtggcaggccctggaaggcatttacccctaccaaaacatctagtaacacacgaactgat + catagttttgctcacacgctaccttggagtaagttgctttaggtaaacgggcacttcttt + caaaaggttttccttcttgctggagctatactgcttatagtaccatattttaaattcata + ccaagccattgtacgcgatgaccattatagatcgaccttgcgacaccatcatatttaata + ttcaaaaagaaaaaaatgcagttctatatcacagttatataatatttatatgtgcatgta + tgttacttgtctttgcacataactcaatgcgtccacaatcttaccatcttgtctttccca + ccactacacactcttttaccgtcgacactccagtcgacgtataacttcgtctttatacca + gggaggtcaacagaaagttttctagttcttacatcccacactttcaacgtggtatctttg + gaacatgacaccagtagtcggcagtccgatgaccacgcaacctggtatacgctggctata + tgccctctaaatgtggagataaacttaccatctctaccgtcccaaagtttgatagagtta + tcaaaagacgctgagacaatatacctaccatcagggctgaacgccacatgattgactaat + ttttggtgaccggtcattcttgctataggcttggtagattttagtgggttccataaaaac + atggtataatcatcgcttgcagtaaccatcatttcttctgaatttccattctttttacag + attttttcataattttccaatgcctttttctgggcttcttctggtgtagaaggcttctta + cctgtatgatcgaatgcaccaatgcgcaatgcgtaatctgtagataaagataagtgatta + acccagtgcgcatgcgacttcaaaatgttgatacatctgccctgcgaattgatgtcccat + acacgtacggttctatcgtgagagccactatacaatagaccttggccgccccatttgaca + caagacactgaatttgtgtgaccactcatcgtatactggcaaactctgctcacagtgtcc + caaatcttaatagtaccatccttagaagatgaagctaatcttggtttggagcccggcttc + acaagatgtataggttcccaacttaaagaagtgatccacttggaatgacctctgagagca + tcacctagacactgaccgctttttgggtcccataatcttatggtattgtccatggatccc + gtagcaattacttctccatcgggggaccaggaaacgcagagaacccaattgtagtgaccc + tttagagtatgcattggcgtttgggtgtcacagtcccaaatccttgcagtattatcacct + gcaccggttaccatcctagaactcgtatgtggtgcgaaggcagaacacaaaattgtggaa + ccgtgacctgcaatggctgatgaacttctagttaccggcttgactttgaaaactgctctt + ggcgtatacagtagcgtgatctgatcttctgtactgttatagcctggttttattaatgaa + gaatataggttatctgttatatcaatcgtcttcacagggtcactggccttcttaccttga + attgtacagctgaaggtatatggcactggatcgtctgaagtaccgttcaattgatttaaa + agttcttctaactgtttctcggagatagcaccgggaactctcagggcgccacctacattg + tcaccagtatctaaagcttggaacttgattgaaacattgggtaaatctttcggaataata + gctacttctctgggaagttgagcctctttcttttgtttcttagaaggtggaggaatcagg + gtggacatctgtatattgtgttagtgtgtgtttatcgaggttacaaatataaaaatcttg + tgatatatgtcttttaaagatactctttttgaagctcatcgcattttttttttttttttt + cattttaaaatttttcaccggtttctcgggtaacagctctaaatgaaatcttgccttcat + caccattattgaattacattacattatatatcgtagtatatcatatttttagacgttgac + cactctatgagcttaggataggtacttgatctttctctgagttgccaaatgctatttcac + ggatttgtatcatccaagggtctatcagtagttccacagcagtagccctcatagtggggt + cttgaaccaaacacctttccaagaagggctcttcccgctgcagtcatttcgtctctattg + ggtagttgcggtattcgacctgcagcaacgtggtacatgatggcccattcattatccaag + ttagaccaaggtcgtctacctgtggccatttctagcacaacacatcctaatgcccataca + tcgtccgctccaagttttcccttaactgccgagcctgaaatagtctctggagccatatac + atcggtgtccccatcatttcattgagggactttgtttctactccaaaatcttgaacggct + gcgttccgcacagttctagtcctagatcctacaacggtacgtgccgtaccaaaatccaca + tattttatgattccattgaaatccagcaagatattctccggtttaatgtcgcgatgcacc + acgccagattggtgcaaatatgccaaaccttctaatagttcgaatgtgtacacttgtgtt + accatttcatcttcaattcttccatgatccaataacgaggctaaagaaccaccctcacag + tattccatgaagatgttaactttatcgcgatgtacttcgacaccatagtactggacaata + ttaggatggtttaacatttccaatacggtcatctcttctttaatcaggggaaaaatcttc + ttcattgttgtggtatcgtgtatctttatttccttaacagctaagatttcaccgttttcc + agattaattgcagagtatacctgtccaaatgttccaccgccaatgaagcttcttttttgc + cacctcattgatacattcgataatgaggatgctagcgatagtaggtatttgtttccctga + tctgtagcatccaataccttacccatttgcctgggattcctcttcatagtcttttccaac + gtctttatagcttccagtctcaacctgctgtttatttctagggtggcctcttcatcaata + ttctcttcagtatcaatattcaatcttgcctgttgcatgccattttcagcttcagtggct + cgtgcacccataacgtcgaaatgtgaaattaataatgacatacatactgataccctctcc + ttcagttcttgaaattgctttacattcagcgccaggatattataaccactagtcatctgc + atcgcaaaatccattgcaagaacgcaccatcggaaagttcgctgatcggtaggatcacac + tcttcaacaaggaatttcaaccagttcattcctaatttaaccatcagaaagatgataacg + tacttcctctttgagctaaaggcgttatatttaaggaaacttcttccaaaatcccttcca + aacatgaatatcgaatttaacaactcatttcccggacattgcttcataaagctacctaga + attcctttgtagttgttcaatactgtataagtaagtttgtaatatgccttattgattttt + tgtagggaggattcaattgaattgagtgaacaacgtttctctatgaatgaaacagaatca + cctaccgtttgattaaacttgtcgatttgataagtcagtgcataattagaaccttgattg + agtaaaatcaacgtatttgggttaccaattttccctaaatccatttcgttggatgcaaaa + aggttgttacctggaagcttatatactggtccatcccaaactactggctctccaggatat + agtacaactagtgcacccaatgagctcaaagcaacttccaattcaaaaacttcattgttc + ttttcatcaaggtgctgatcaaaagggtctgttgaaaaattcttctttttggactgatga + gcatgcctgtccaaatcacttgacgaatttgctactactcggtagtaaggaattcctttg + gaatcctcccctttcgatactagaatgttcaaatctgtttcttttgttgttatatcatac + acattcaaattatttccaatgtcaagctttgggaccaaatcacagccaatggatttattt + cgcaaaattcttaagatggtatcattgtcacaacctaataattcaggagcagcaaacata + tatacaccactagactcaaaagtgttaccggagtataccaaaaaatagtgagcatctttt + aactttttaacaagttgtgcattatgattaatctgataaacagcagaattttggaacgcc + ttgactagaatattgctgaacctgttcagctttcttttcatggcacccaaattttcgaaa + atggaacttagccatttttctgcttctgaagcgttagcaaatgttggtgggaagttttgt + tgtttcaaaagatagaattgcaatttatgtacgagtctcaaagttaacttgagaaattca + tttggaatcacggtttctgcaccgttaatatagtgaccggtgttttttaggtgatcccag + tatttcaagagtagatcgggtgctttgaaattttgtttactggaatcaatcaactttaaa + ttcaacagaaaaaataaataacgaatggcttctattacagtattttcgaacgtcgggtca + aaatccacatcgaacggcaaattggagcaatattttgtcagtgtgtacttcaattgcaca + gaaagtcgaataaaagcgttaaaatcatcgatcatttgatcgatcatcatcattgttgga + tttttaagctttcgtgcatatgacaatctagtcaaaataacttctttgattaaatagacg + gggaacgccagtagtgatcttaacctttcataaggatattttatgttcatctttgtcaaa + gtttctctgtagactataaaatgtagtttcagtttgaacatccaaggtgatagcggatag + aaaatttttttttggaatatagactcgatgttcttttccttcatcaattgttctgcaaga + atcttcattttgcagttccattggggcacaccatcaaaatcctcttggcaattatccgaa + ctacttatcggtgagaaacggtttatccagtcgtccaactcttgcctcctcaacgttaag + ttcgttttaaagttcagccaagaattcattacgtctattctagcggtaaattctgcagta + ttggtgattggtttatccttatacatttttttcaagttaggccaataagagacaacgctg + tgatatctgttcatgaggtcttgtaagatggcctccagtgcatccaggctcaatatcttg + ccctgtggaatttgaaacttcattatctcttcaaaaacggaatctgaactatctcttaaa + tatgtaagcgactgttccatctcttgcatggtcctcccattcagccatgccttcaattcg + agccatatctcatctgagagctccttatttaaccctggtttcttgacttggttagcaatc + ctcgtcttttcacctttaacaatatctcccttcagcactctggccagcattgactgccat + ttgaaccgttcggtcgcaatttcattccaagctagttcatcactgtctgacctcattttc + ctgcaataaggtgtagtggagaaaaacttgacacctgagtgcaggtcttcatcaattctg + tcgccattagacaaccgaataatttcgtcatcaccgtcgtcttcctcgtagcggttagtg + agttttatgcccctcgtgtagtagtcatctaaagcggtcttttttagctttttcaagtat + agcgattcattgaaaacgtatcggtccttaaaagtcagctgactggggccgtcgcttatt + gtttccgagccacccaatgctttcaaatcagatcttgatagtttatggctggaatgactt + gataatctcttcacgagcttacttgagatggtatgaggagccagaactctcccgccttca + gccgctttttgttgctgtgtattcagtatatccatcatcattttcacctacaaggaacta + ccttttatagccaccctaagtaaaacaacattagcttagcatcctcaattcttatcgtat + gttgttgctgctatttttatcctattgttcctggcatcgcttttttacataaggtaccaa + ggcaagagaaaagacccgcgaaattttcaattcgagacatagggttaatacgaaatatgt + taaggtctagtttccaaaaaatgaagaaaatgtgattagacatcctggggaaattaggtt + taaatagggcgggcgctacaggggttttcctaacaaatttcaatgataatagtggcatca + tcatcgtcatatccagtgtaggtatggactagaacagaaagcaatttgcagcaagacaat + atgactacgacggtacccaagatattcgcgtttcacgagttttcagacgtggcagaggcc + gtagctgaccatgtagtccacgcgcaagacggtgcattggctccaaagaacgagaggaaa + cactctgttcccaacatcagcatgaatgcactggatatgacgagagaggcctcttgcaaa + agcacagcatctgccgcggaagggaaaagtggtagcagtggtagtggcagtggtagcagt + aagcccaaaaaggagaaacggttcaagattgctctctccggtgggtcattgatcgaagtg + ctacacgaaggtctgctaaaacgagacgatgtacggtggggagactgggacatttacttt + gcagacgagagacttgtacccttcagctcgaatgaaagcaattatggatgcgccaaaagg + aagattttggacctgatagacacggcgaagtatggaactccgaaggtgtaccacattgac + gagtcattgattgacgacccgcaagaatgcgttgataactatgaaaaggtgctaatccgc + gggtttgccggtagagattccgtcaaacttccgatgttcgacttgttcctgcttggttgt + gcccccgatggtcatatcgcatcactcttccctaacttccaggacaatctacgtgagaaa + cttgcatgggtggtgcccgtggagaacgctcctagtgggccctcgaccagaatttcgctg + actatacctgtaatctgccattctcacagggttactttcgttgtcgaaggtgcaaccaag + gcgcccatcatcaagaccattatggaaaggcctgaaaagggcctacctagcagtattgtc + aacgaaggtgctgctggtcgtgtatcatggtttgttgacgacgatgctcttacggacgtc + ctcgtcaccaaaaaaaagtataaattccaccaaggtttgtctatttaaaccatgcatgtg + tgtacatacatacatacacacatacatacatacatacatatatttatgtatatttatgta + tatatatatatatatatatgcgtaattatgcagatttctccttgtgtgctacagcgggta + ctctgacgccagatcatggcctcgagatcgatatacaaaccactgcatgataaagatgaa + gttgaatattaaagttaccattgacagtcccacttttccaaaatttgtcacgaacgtatc + cagactgaaaccttgatcgttagacaactgccaaatcaattggagcagcgaggcgatacc + gccagtgacatctagaaacacaccctgaatggggaaacaatccatagattttcttgtcga + gttatgcgtcacttgtgggatgtacttgattagtgacatcgatattttgagtaaaaacaa + attgttgcaatacgccagagttcttgagttatgccaatcgtaccagtagttggagtacat + aaattgtacggttagcagtgaaaaaatggccagtgatgcgagtaaaatccttaggtacca + tggattcatcttgcggtgacctttacctggaaatcgccagattctcgctccagctaccac + ctgggtcaataagacaacattcatcaagcacccatgcaggcaataccagaaatcaaattg + cgtcaacttgggcctgcccaagtcagactcatcacccgtcattttccagcagtacaattg + caaaaatatggatatgaccaggtaagagtaacctgccgtatttaacatgacaaaatccat + cgatatcgcgctcgctgacttatggcgccaattggtgattatcggtggatacatcgatat + cgaccatgacgtaacatacacgatacctagtatatcgtctaacgacaccattttgcttcg + cgtttgcttttgtttgcttttagtttttcttcccttgtttacatatggaaactcacctta + aaatttcaagattatcaaaaaatataaaggattccgctattaaagggcaataccacaacc + tcaaaccaaatcacctaaaccaaatcaaagatggaagcggaaaaacaatctgatattaag + ggaaccattgctttcgatactcacggtaacgttatagagtctaccggcgtgggcagtcaa + aggatcgaagatatcggtgatttatcgaaagttacgctggatgcagaaggttttgctcaa + gttcaaggagattcgctactcgtacacctgtacaaacgcaatgatatcactttagctgta + tataccagcgctcaataaattctttccttccctttcgacatagctatgtctgtatactat + ctaaaaactaaatatcacaataaacaaagtactaaaataaacacccatttaaataaagcc + agatcctgaccctccaaatccgccggatcctgaacctccaaatccgccagatccaggacc + aaatggttcgtcgtacttggatccaggcatccatcctggcggcatatcttcccgtcttgg + ggcaggtcttctatttggatcgaaaatcataccgccttggctacttggattccccagagg + attgggaagccctccagaccaatccggatggcttgtgccaacaggatacagatcaccatc + cgcataccctcctgctgggttagcgaagctcggttcagttaccgtaagtccgggatatgg + gttcaacggtgctgttccctggttttgtcttgcttgtgcctggtactcatcgtcaaattt + tggcatgtccgcactacctgcagtttgtcttttaagttgtgtcattataggtttcaggtc + tggctccacaggtaagctttctctttctagtggccatttcaattctgtgggcaatttaaa + atcgctgtaacccaccacacatgccatggtgatcgacgtaccatgacgaataacaacagt + atacttgtcattgtcatccagagtctgaatcaccactagctcagagagaaacgatgttga + acccttctcactgggagaatctttaatgcgaaaactcacttggcatgatttggttccctc + tgtatggcgtgacaccacctcagttttaccgatggactccaacactagttctgctaccag + ttcaaccttattctcaatcattgtttaactcttgcgttactatggtggtattattgatgc + taatacgggttttttatgaaaaaaaaaggataacaaacttatataaacttatataaaaac + atatacaataaatgatctacaaagggtaggaaataaaaaataagggagaaaaaaaaatac + atgcgtaagtacattaaaattacaggaaaaatcttactttagttctgatatttcaccatc + gcgatacaccaaccccataacgtttaggaacaaattcaaatcttggtatagtttctcttt + attataaataatagattttaactctgtgtctaattcttgagacctctgaggtaatacact + caattctttctctttcttttgtaaggtctccgcctcgttaatagtggcaaagatttcatc + acgtacttcatcaataatgattctctggtggtttagctttccacttagtgctaaactagc + taggaactgccaaagtatgcttcatccataaacttgtcattttgtaaacgcatgatgtgg + acgttatattcccttggagggaaaatcagctgaatcttactttccaatgaagtgaataat + ttatcataaatctcattccatgtggagatttcaggagaggaaagaatattagatcttgat + gaatcttgcttgattaatgcggacgagaaatcaaaatggtgatcaaatttagaccaattt + tggaggtggtcaagaacgaaacgttgttgtttctgattaactgtagcaacagacccataa + tttcgataaaattggagttattagacaaaaacgagacaatgatctttaatatgatcattt + ggaacagatcgactttcttcaattgtgtcaaagttggttttggtgtagtcttgtaggaac + tcaatatgatgatttgcaagtgtgataattcattgaagattttttgcaggattttcaatt + tttgctgcttatccaagaaattaaaaattctaggcataatcttgatacctttatcaaatg + atagcatcgaaatgaaagggtttacgtcatatgaactgtcgtcgatgtgaagggcctccc + acattgcagtgctgtcagtttgctggccattcctcaagttagcttccaagtctaaaatct + cttcataaactgtttcaattaactcaagaatgaattttttgcccccagatttgttcaaat + ttgtggcaccattaccgttgttgaacgcgtatgaagatcttcttctttgcttagaagcaa + cagcagcggcagaagagcccgtagaggaggaccctgaagaggccaagtccacgttggtca + gagagtcagtgacgtcttttagcgcatcggggtcagaagaaggctgttgtctggggatct + gcagttgtcttcttggattcttgctgttcaaagtggaagagattttgcccagaaccgtgt + ttgcttgcttattatcctggctagagttaccagcagccgcttgttgatccttcaacttag + aaggtctttccttagccacagtgacagccttttctacttgactttgcattctctgtaggg + caatatcggttctcttatagcgaccaccgagtctgtgtccagaatgttccaaatacgccc + tagcaatcaaacctttgttggattcggacgtgataccgcctctttggataatcttgtaga + cctggaagtagaaatcctcattgtaagggtcctcagtgacaatttgagacaactgatatc + tggtgatgaagtccttatcacgaggagtcattaaacctgagtatttcaagattttctcca + ctttggcatgacgaatctgcaaacgtctttgctcttcaggcgacaaatctctcttcgatc + tgcgtccactttgcagaggcattttagttgcgccaaccacagggcctggagaaggagtgt + tggacataccgatgggtgaaggcgaagcttgaactggaacttgtacaggacccattggca + tttgattcatattcacattaacgccaggaggtggaggtggaggcgcaaattgtgagggac + ctggttgacccgacgcaggctgcattggaaattgctgctgaggcggaccctgtgggagac + cctgtggatgacccatggcgtggaaattcataaactgttgctgcatttgacgttgttgtc + tttccaagtcttgcatcgacaagattggctgtaggggggccatttgcgcggagcaggagc + cggagccattgtactttggggtgaaggcgccattgctggaggtggtgcagtagaccacaa + agattccattggcttcaggtctagaggtcctgctgctgccgttccatcggtcctagggcc + gctaattccttctgcagtgtgctcccaaggcttgaaatcagggtatacttgttcctgagg + tttcgagcttggcacataggggtgaagatcatctgggggagtcagcaagtatcgaccaga + accgttattcatcgcgttattggcttacctctatagacctttttttaatctttcgattag + catcaaaaatctcagcattttttcagaaaggtgtttaaaatgcgctataaagaatataaa + aagtaatatatatgtatacatactaataaaaggaagaaaaaaaatacaaaagatgattat + ctaggacttgtttcacccaggggggtgtttagtttaattttgtttcgttagattgtgcat + tcattagtttggtttctctttggggccagttctgtcaacattgggccagttgcccaattg + gaaagcctaaccaccacgcaagtggcgttgtctgagtgtttagtggctattgcctgaata + aatttgattgtctcctctgcaacaaattgaggagtcgcctttttcagtccccaggagttc + accgtagaggtgatgaggtcaacaacctcttggtctgcaagtttattggtgatgccgtcg + gtgacaagcgccaggaagcattcatcaccattgaagggcagcttcgacctttcagatcgc + ggcaaatgcaaggtatttccgacaagaaacgagaaaatatccggctcactagatatacca + tacggtttcccagcgacatcaccaaacgatctagtgttggcgaaattattcaaaaacctt + gtctctccaaaggcgtctgggtccaatcgagaagggtctatgctcaaacggtgtcgttcc + ctactggaattgatgtggtgagttgttgttaatgcgtgtgcaatcccgtcttgatcgcag + aggatgatcttagagtcgcccacctgcgtgacaatgagtttgagcaatccagagtgcgat + atgatccacgagtcatcgtctttcccctcatcaatgggtgcattcggatcatcatacgga + tatagaaatatggacgacgctgtggaccccgctgcggtgcccaggccgcagcattggtcg + agatcgaacctggcaaacccgtagaacaatcgcagacggtctagctcagtgagcagtgca + attttgtcaataatattccccattttatcaaaaatcatcctgccaccattttgtccaagg + atcgcactatcgccctccttcatcagcagcagttcttgtttcgagttgcagttctttata + aacttgttgtagaatttttcaaacgtggacagcttatgtttccagtaatttccctcggga + aaccttcttgcatattcctgtaccaacttcttcaagtcctgcttgtcccaggagggttca + gggaaatccaggctactgcacaatttatcggctaacaactgtgacagttgcgcacctttc + tccccatggccatcgaatacagagcagttcagggcacttccatacgcattaggccacgac + cgcatcattttaatggagtaattgtcctggttctctaaccggttgatcctactcgtggcg + tgacccacgtaccctggaagaccccgtaacaagtccagcttcagcccaacattttgcgac + ggcttgcaatacgcataagcattccccagccgcatcaaattcccctcttctgatgcttaa + ttgccccgattgcagcctgttattgatactattgttatcacttccctatttgttcttctc + gcattacacgaaattttttttttcctcttcctctttttaaagaaagaaacaaggtgaatg + aaaagaaacatgtacaccttgaaggaagcaaaggttttaaccggaagtaagagcagcaag + aagcactagcaatgtccttctttgggttagaaaatagcggtaatgcgcgggatggtcctc + tggactttgaagagagttacaagggctatggcgagcacgaacttgaggagaacgactatt + tgaacgacgaaacatttggtgataatgttcaggttggtaccgactttgattttggaaatc + ctcacagcagcggcagcagcggcaacgcaattggtggtaatggcgtcggtgccacggcta + gatcatatgttgcagctactgcaggcagagatcaaatcctagcaaactttgtggctaagg + gcttttaccatacaccaatggtaaatttcgagtccatatctgcgagatcatctgttcatg + aatcattagtcactcaatccaacattctttcccagcaattcgacaaaattatcaagatta + gagaagaccacattaataagatcccctcaaattccacgacgacattacacgggcctggtt + ttcagttgcctaatagaataacccttactgatcatagaaaggaaacgtggttgcatgaat + tgagattcgtctcacacttcgactggtcaaaattggcaagtttatacctcacggcttgaa + aagaaggcaagtcatcgagcagtgctatttaaaatttataccattgaaaagggcgatttg + gttgataaagtgctgctattttatcgaatggaaatcgaaccagaaaaagaagaggtcaaa + tgctgctggggcagatgatgccatttccatgcacctgctaaaggactggacggatacctt + tgtatacatcctggaaaagctcatctttgatatgacaaatcactataacgattctcaaca + actgcgtacgtggaagaggcagatttcttattttttaaaacttttggggaattgctactc + actaagattgatcaataaggaaatctttcatcattggcttgtagagtttataaataagat + ggaaaacttcgaatttttgccattatctttacatattttgatgattttttggaacgacat + ctgccaaattgatacaaatgctcctgttgcggctacaataacatcaagtcaaaaagagcc + cttctttctggtaacaaaaatcactgatatgctattgcacaaatattatattgtttccag + cagcaaatcaatgataaatgacgagaactacatcatcaatgatataaagaaaaacaacaa + gataaagttgaatattctcaaaatattatccagtttaattttgaaaatttttcaagaaca + atctttagaggtgtttatatttcccacatctaactgggaaatttacaagcccttactttt + tgaaatagtctcaaacgccgacactaatcaaaattctgatatgaagaaaaaattagagtt + aattagttacagaaacgagtcattgaagaataattcttctatacgaaacgtaataatgtc + tgccagcaacgcaaatgactttcaattaactatcgtcacctgtaaacaatttccaaaact + atcatgcattcaattaaattgtatagatactcagttcaccaagctactggacgataaccc + tacagaattcgattggcccacttacgttgaccaaaatccccttacaatgcataaaattat + tcaattaattctctggtccatacatccatcaaggcaatttgatcactatgaatctaatca + actggtagcgaaattattactattgcgaataaattcaacagatgaggatttgcacgaatt + ccagatagaagatgccatttggtcattggttttccaattagccaaaaatttttcggccca + aaagagggtggtatcatatatgatgccttctttgtatcgcctgcttaatatactaattac + ttatggcatcattaaggtccctacgtatatcagaaagctaatcagttccggcctacttta + tctccaagattccaatgataagtttgtgcatgtccagctgttaattaacttgaaaatttc + accgttgatgaaaagtcaatacaatatggtattgaggaacgttatggaatatgacgttaa + attttatgaaatttttaatttcgaccaactcgtggaaatcacagaacaaatcaaaatgcg + aatactctccaatgatataactaatttgcaactgtcgaaaactcctctgagcattaaaat + catggttgcagaatggtacttatcacatttatgttccggtattttatctagtgttaaccg + cacagtgttgctaaaaatattcaagattttttgtatcgatctggaggttttccaccactt + ttttaagtggatcgagtttattgtctaccatcaattgctaagtgatatagaatctctgga + ggcattgatggacatcttgctatgctaccaaaaattgttctcacaattcattaatgacca + tattctttttacgaagacgttcatattcatttacaagaaagttttgaaagaaaaagacgt + gcctgcttataatgtgacttcatttatgccattctggaaattttttatgaaaaacttccc + ttttgttttaaaggtggataacgatttaaggattgagttacaatctgtttacaatgatga + gaaattgaaaactgagaagctgaagaatgataaatcagaagtcttgaaggtgtattccat + gatcaataattcaaaccaagctgttggacagacttggaattttcccgaggtgtttcaagt + aaacatcaggtttctactacacaactccgagatcattgatacaaatacaagcaaacagtt + ccagaaagcacgaaacaatgtcatgcttttgattgccactaacttgaaggagtacaataa + atttatgtccattttcttgaaaaggaaagactttactaacaaaaatttaattcaattgat + ctctctaaaacttctaacttttgaagtgacgcagaatgtgttggggctcgagtatattat + tcgattattaccaataaacttggaaaataatgacggctcatatggtctgtttttgaagta + tcataaagaacaattcataaagtcaaattttgagaaaattttacttacatgttatgaatt + agaaaaaaaatatcatggcaacgaatgtgaaataaattattatgagatcctattgaaaat + tttaataacttatgggtcatctcccaaattacttgcaacatctacaaaaatcattatgtt + gttattgaatgatagcgtggaaaactcatctaatattttggaggatattttgtactactc + aacttgtccgtcggaaaccgatcttaacgatattccattgggtagtggacaaccagacaa + tgacactgttgtaaccaacgatgataaaagtgacgatgatgatcacacagtcgacgaaat + tgatcatgtagaatattacgttatgatggactttgccaatctttgggttttccaagcgtt + tacctgtttctgcatcaaaaaaatcatggagaataatgagccagcaatggcaatggaaga + cttgaagaacttcatattccaaattatcgaaataactaattctaatgatttatgttcaca + aatatttgaccaactgaaggatatgcagaccattgagatgataacccaaatagtggagaa + agatttctgcacttcttgtttgcaaaacaacaaccaaaagatagatgataattacatcgt + tgtggtgatcgagattataacgtcattatcgatgaggtttcaaagagaaacttctggtat + gatagttatttccatggagaactatcatttactaataaagatcataagacaattaagtga + actgaacgaaggaaatttatctaagagagaaatccaaatagatgccgtcttgaaaatttt + tagctttcatcaggattccattttccaacgcatcatcgctgatttatcagctgataaacc + cacaagtccattcattgatagcatatgcaagctgtttgataaaatatcatttaatttaag + attgaagctgttcttgtacgaaattttgtcttcattgaaatcattcgccatctattcatc + cacaattgatgccccagcattccacacaagcggtaaggtcgaactaccgaagaaattgct + gaacttaccaccattccaagtgtcctctttcgttaaggaaacaaaacttcatagtggcga + ctacggggaagaagaagatgcagaccaagaagaatcgtttagtttaaatttaggaatcgg + catagttgaaatagcgcacgaaaacgaacagaaatggctcatttatgacaagaaagatca + taaatatgtctgcacattttccatggagccgtaccacttcatctccaactataataccaa + gtacacagatgacatggctacaggcagtaatgatacgactgcgtttaacgattcctgtgt + aaacctgagtctttttgatgctcggtttgagaggaaaaatccacattgatctcagaatat + atccaaatggataaattataaatttaccaataacagtaattatgtgtcagttttaatacc + caaccaattgattcctctaaatgataccgtaccaaatgacatgtttgccaccgattataa + aactggggtttatgagcgatggttaaggaggattaagacaaagtgctcatccgcttacaa + gatcagtactagcgtgtaccagtctatttaaactggaatgatcaccccaaagggaacgca + cgatgctgtggccaagtttcaaaaaactgacctgcatcaagatctcgattacatcgtact + gcaacaacgaagaacacagctagagacgcttattaacgaaagagaatcttttgtcaagaa + tctgtgttctcttttccacaaaattcaaaataccaagaattaccaggaatttgttgatgt + attggcggagaatagggatttactgcgagaaattttcactgtagagaacggatttcaaaa + gcaaaaatggattagcaacgacgacattccccagatagactgggacaagtttgccctaga + tatcaacgcttatatagcagagaacgatcaattgttggctttgtatgaagatggcttatt + atgatcaaatagcggctatggacatttttaaacatatatttatatatatatacatatatg + cgtatattgcataaatcacaaagaagaacaacgccctagatatagtgacccaaaatatta + tgtttaagttactggttggggttcatgtacatttttcactatcttttaattccaaatgtg + gattgtgcttctgcagattttgttgcctagtattctgtgatggaaaaaattgccccgatg + agatataaaatgagctagccccctaaccaaagaaagcaggattggttagtacatagagaa + accaaagctgttatgttgttctataagcctgtgatgaggatggcggtgagaccgctaaaa + agcataagattccagtcctcatacaccagtattactaaattgacgaacctaacagaattt + aggaatttgatcaagcaaaatgataaactagtcatcgatttttatgctacttggtgtggc + ccctgtaagatgatgcaaccacacttaacgaaattaattcaggcttatccagatgtaaga + tttgtcaagtgcgacgtggacgaatcaccagatattgccaaagagtgtgaagtgacggct + atgcccacctttgttcttggcaaggatggccaactcatcggcaagatcattggagctaac + cctactgctttagagaagggaatcaaagatctataaattttatattcatgagcacatgta + gtttttttttttttagttttaaatataagaaaaaagatagtatatatatatatatatata + catatacatataaaaggaaagtaagcttatacaatacacaaataaattcatatcaaagaa + taatgaaccgcaatattcagaaacacaggaaaaggaggggaagggatgaatggtgaggaa + agtaactgtttgttttaaagcgtacctggatcataacataacaaataagtagataaaaaa + gaatagagagaaagatataatgacaatgaattgaagaatagtttagttagttacatttgt + aaagtgttccttttgtgttctgttcttaatttggcgctatttttttatacttccaaatcc + ttgctttacaatcaccgctaccagtagcaaaaacgttatattctggacccagcggagacc + cgtttgccacagccacagatataactgaattcctatgaccttgcaacatcaataacggat + tgccggatttcttatcccaaaacaggacaccacgatctttggaaccggacaagatgtact + catcattttgtgtggtggccacggacaatacaaagtctttatgcccgatatacgtaactt + cacaagtgccggaatttggagttttcgaatcgctcttgttgtttgcattctgcaaattcc + agagcttaacagatctatctaatgagccggatacaacgctttgtccatctctagtgaaga + caacgctataaacagagtccttgtggcctgtaccggattcgttttccgaatctagtcttt + ccaccaagaatccggtctcggaatcccaaactctcacagcacgatctagagaaccagcag + cgatgtatttaccatcacctggtgatacagcgacggtggtaacaccatcttcaatggata + aagtcaatgaacactggcctgtacgtaagtcccaaatacgaacggtacggtcaccagaac + cggagactaatttgtcacctgagggaaagtagtccaatgaataaatatcttgttcgtggc + cttgaagaatcataacaatctttctattttcaatatcccaaattctaatcagtctgtctt + cagcacctgttgccaaaaatttcccatctggagaaaaacacactgaacggatatacaagt + cggatgatggggaagacgaagtgttcaggttttccacatcttttgccaattctgctgccg + aagtcatcgctgtggtagtaattgtggtggtagtagtggttgtcattgtattgttatccg + tggacgtggtggtgttattttcagtgatcgaatttcgatggttattggcagcagaatcgt + cagatagacgggccaccagagaaccatctgaaacgcgatacacttgagtagttttgttgc + agcctgtggctaagtattcaccatcgttactgaacttcacgcaacaaacaactgaagtat + gatccaaagatttgtgtaactcaacgtcaatttctcttggtagtgccgggttgtataaaa + tataataatcatttgtttgcttcttcagagcatcgggaacagactgggaatctagatcca + aaaggaaaggtgggataggtttagagtggtttgctctttgattataagggactaagtagt + gatcctggtgcaaactagccggggtggcgtcttcctccttaggtttgatttcagtttcag + ttgcggtggtagtggtggccgtggtggcggatccggtgtcatttatcttcgaggtattat + tattttccggttcagtttctttcaacgtagattcaggtgccttgacagagggtaaagtag + tggtctctttagaagtaggagatccgttgatggctgtgttactcaatggtgccacggaaa + cctggggaggtggttgctgttgttgaagttgctgctgttgcagttgctgttgtggtagtt + gttgttgggcgtttgaggacaccacaggcaaagtggtggtaggcaactgtgagccaacca + gattaggacggctagcttgtactgggaaggccgatggcgaaccagtagttgtgtttgctg + ctggagtggcggtggccgaagtagtagccggtggttgttgcgcaactggaacagatgcag + atgcagcggctagctgctgctgttgctgttgtaaatgctgctggacctgttgctgctgtt + gctgttgccgctgttgctggacggtcaaagatgcaatttgatggtctctttgctccagcc + ctagtttcaagtgcttgatctcttcttcgtacgcgtccttcattttcctgtgagttagtt + ccagttcgtagacggtgtttcttatctgctgcatctcagccagctgctggttcattttga + aatcgtaatccttttggttttgaagacggtaggtatttgcctcttgtgagacttggagaa + actcctgtctgatggcatcgagaagctcattcagcttattctgcgtattcgaaacgctgg + cagtcatattggtttggatggaaagctgatttctttcttcccctgcttatcaaaaaagac + aaaaaaacaatagaaaaagagtagtggtgtgcgaaaggtaacttggcgtgaaagaaaatt + aaacaattgcagaaggacagatagaggggcacgtgttcagccagttgttcttgcttcgct + gatgtttatcaattgggggtagatgggggaatttctctctatttgtttacttatgtactt + gttattttgttttgttgtttgttgctttgacgggagagctttttaattttttcgcagttt + ttcagtgttttttgaaaagtacgaaaaattaaggtccgtcggtagcgataatgtaagagg + gttctgtctctaaaaatgttagggtgaaagttccaaaaaattattaactcctagattgct + cagccaaaaagaagggtaaattagggctttcctggtttatttatttgtttatgttgttct + tatatacatatgtttatgtctttttatgtacaaataatgatgtttacaatgaaacatctg + ttatgctatcaaaaacgtcagcgcattgttttatagctgaagaagttacaacggataacg + gtctgatttgcggcctggcaatgcttggtaaaactaaatatcaattttacgaattattta + ctgtatatagtattcagtccctcactcaactggcgtcaagagtgaagaagggcggcctca + ttatggctcgccttattctgttcaccctctgcgctcttcccgtattatttcattttattt + tgtttatgcttcaatatcttgtatttgtttacattgaaaaatgattggaaaaaggtcaca + ttgaaatcaaaacagtgaagaagcagacagacaagaaggtcattagatagataaacagga + acaataaaaaaaagaaagcgaaacaagacaatccaacgtgcgcaaaatggatccattgac + tgtatacaaaaactcagtgaaacaacagatcgattccgcagacttactagtggcaaattt + ggtaaatgagaatttcgtactgtcggagaagctggatacaaaagcaaccgagatcaaaca + gctgcagaagcagatagactcgctaaacgcacaagtcaaggagctgaagactcagacttc + ccaacaggcagaaaattcagaagtcataaaggacctttacgagtacctctgtaacgtacg + tgtacataaaagctacgaggatgattctgggttgtggttcgacatctcgcagggcaccca + ctcagggggatcttccgatgattattcgataatggactataaactcggatttgtcaaggg + ccaggcccaagtcacagaagtcatatatgcgcccgttcttaaacagcgatccaccgagga + actatactcgctacagtcgaaactaccggaatacctcttcgaaacgttgagtttccccct + ctcgtcgctaaaccaattctataacaaaatcgctaaaagcctgaataagaaaagagagaa + aaaagatgaaaccgagtaagctgctacataatgtctatatatctacacataaaattccga + ttattcctttgcatacctgatttgcccctcagaatccacaaccagacttttcaagaaggt + cttttttgcccctttatccttcatggttttcaaaattttgtacaacgacttgcccttgtg + aagctcgataccgttcgaggtcttttccactttctctgccttcttgatcacaggcttctc + cactttctttgcaggagttgccactggctggtgctggtgctgcttctgctgctgcttctg + ctgctgcttctgcttctgcttcttgttgcccttgtacaacgctttctggtacttctcgtc + ctcgctgatgcaagacgtgtgattcttgtaactcacgccatcttcaaacgtcttggagca + atctatgcatgtatagtacgcgttaggacatctataataatgcttttcggtattcttctt + gggcacagtatcattacacacctcacagttgaacgtaaccatcctgtaataacaaatatt + tcttgactgagaccgtttgctgttgtatacagaatactcttagagctcatcgcaagttaa + aaattttcaattttttttcactttttcccgtcaaggcaaaaagcaaccaaaagagaatga + acctttatttttgatttatttattatgagatgctgctagtccactcatctgcatcaatgt + agtagtcacaaagctaatatttagacgttactttgatatctctgtccatgagagcactta + ttttaggaagttaaatgagacagtcaatagttcacaatatcccgtcagcaatggagggaa + aaggcattccttttccataggattttaatcgttttcaagcatcatacgccctcgaggaac + tcttgttttcgctttactatgcaaccattgatgtatttctgtataaatgtgcgtcacgtg + gccttgtgtctcttatttccacttgttttttcacaatgcggaaaacctcgattaaagtag + aaaaaaaggatataataggagtataccatattggatagttcaatctataaacaaacaatc + gcataaccgcacgtatatacacgcacacacctatcaatcacaatggctttggaacctatt + gattatactactcactcgagagagatcgacgcagagtacctgaagattgtcagaggctcc + gatcctgacaccacctggttgattatttcacccaatgcgaaaaaagaatacgaacctgag + tctaccggttcctcctttcacgatttcttgcaattgtttgatgaaaccaaggtccagtac + ggactggcacgtgtgtccccaccagggtcagacgttgagaagattattatcattggttgg + tgtcctgattctgcgccattgaagacaagggcctctttcgccgccaattttgctgcagtt + gctaataatctgttcaagggttaccacgttcaagttaccgccagagacgaggacgatctt + gacgaaaatgaactgttgatgaaaatcagtaacgcggccggtgcccgttattctattcag + acttcctccaagcaacaggggaaggcttccactcctcccgtgaagaaatccttcacacct + tccaagagccctgctccagtttctaagaaggaaccagtcaagactccttccccagcacct + gctgctaagatttcttcccgtgttaacgacaacaatgacgacgacgattggaatgagcct + gaattaaaggaacgcgacttcgatcaggctcccctgaaaccaaatcaatcatcttacaaa + ccaattggcaaaatcgacttgcaaaaagtgattgctgaagaaaaggctaaggaggaccca + cgtcttgttcaaaagccaaccgctgctggttccaagattgatcctagttctgatatcgct + aatttaaagaacgaatcaaaattaaagagggactccgagtttaactcctttttgggcacc + actaaacccccctccatgacggaatcttcattaaagaatgatgatgataaagtcattaag + ggttttagaaacgagaaatcacctgctcaattatgggccgaaagaaaggcaaagcaaaac + agcggcaacgccgaaactaaggctgaggcaccaaaacctgaagttccagaagatgagcct + gaaggtgaacctgacgtcaaagatttgaaatcaaaatttgaaggattggccgcttcagaa + aaagaggaggaagaaatggaaaacaaatttgctcctcctccaaagaaatcagaaccaact + attatctcaccaaaacccttctccaagccacaagaacctgtgaaagctgaagaagccgag + cagcctaagactgattacaagaagatcggcaacccattacccggtatgcacattgaagcg + gataatgaggaagaaccagaagagaatgatgatgactgggatgatgatgaagacgaggct + gctcaacctcctttgccttcgaggaatgttgcgtcaggagcaccagtgcaaaaagaagag + cctgaacaagaagagatcgccccaagcttaccttctagaaactcgatcccagctccaaaa + caagaagaagcacctgaacaagcacctgaagaagaaattgaagaagaagctgaggaagcc + gctccacagctgccatcaagaagctctgcagctcctcctccgcctccaagacgagcaact + ccagagaaaaagccaaaggaaaatccttgggccacagcagaatatgattacgatgctgca + gaagataacgaactgacctttgtggaaaatgacaagattatcaatattgaatttgtcgac + gatgactggtggctaggggaactagagaaagacggctcaaaaggtctcttccccagcaat + tatgtgtctttgggcaactagaatcacacgtcagcgtcatgctattatattattcttacg + taaaaaaatacttacagagacgttaaagcttttgtatagatactttcacgagccttccct + ttcagtaaaatgaacaaattattttgccttgtccttctttgtttcaagcgtttatatgac + tcctctacagtattatttttcaacagacggtgtgcccgtacggcattgtcaaaacctcga + acataaaacaacactgaaacaaacttattgcactcctgcatcgcatacagatctgatact + tactacaaccataatgctgttttagaatcgcatcaggtgtttaccagaagtgtatggtac + atggtgctttccttatgctcactatttcggatttacttctagtctatgtatagatttgtt + agagaccagttttaagctacaatggaacgttaagattaagaaatgcatgaagagtctccc + agtagatatataaagctgaattaaattcagtacaatggaagtttccacgtctttctgtat + tttcatacctttaattataggaaaagcacactacaaaaatacgctatcatctaccgaata + taactgcaatgaactcatttgcgtcattaggtctgatatattcagtagtaaaccttttaa + ctagagtagaggctcaaattgtgttctaccagaatagtagtacttcactgcctgtcccta + ctttagtatccacctcaatagcagattttcacgagtcctcatcaactggcgaagtgcagt + attcatcctcttattcgtatgtgcagccctcaatagactccttcacttcatctagcttct + taacaagttttgaagctcctaccgaaacttcttccagctatgcagtttcttcctcattga + taacttctgatactttttcttcatactctgatatcttcgatgaagaaacaagttcattaa + tatcaacctcagctgcctcatcggagaaagcctcgtccaccctttcttcaactgcacaac + ctcataggacatctcactcttcctcttcattcgagctaccagtcactgctccatcatcct + ctagtttaccgtcctcaacttcattgacatttacgtcagttaatccatctcaaagttgga + cttcatttaactcagaaaaatctagcgctctttcctcaaccatagattttacttcttctg + agatttcaggttcaacatctccaaagagcctggaaagtttcgataccaccggtactataa + cttcatcttattctccttctccttcttcaaaaaattctaaccagacctcactactcagcc + cattggagcctctgtccagttcttcaggagatttaatattgagttcaactattcaagcta + ctaccaatgaccaaacttcaaaaactattccaactcttgttgacgccacatcgtcattac + caccaacattgaggtcatccagtatggcaccaacaagtggttctgattcaatctcacaca + actttacgagccccccctctaaaacaagtggtaactacgatgttttgacttcaaactcaa + tagatccttctctatttacaactacgagtgaatattcatctacacaattgtcgagtttaa + atcgggcctcaaaaagtgaaacagttaatttcactgcttctattgcttccacaccatttg + gtacagattcggctacttctctaatagaccccattagttcagtgggttctacagcatcta + gctttgtgggaatttcaaccgccaattttagtacacaagggaactcgaactatgttcctg + aatcaactgcaagtggaagttcacaataccaggactggtcaagctcttctcttccgctgt + cacaaaccacttgggttgtcatcaacacaactaatacacaagggtctgtaacgtcaacca + catccccggcttatgtttctacggccaccaaaacggttgacggggtgatcaccgaatatg + ttacatggtgtcctctaacacaaaccaaatcacaagcaattggggtcagttcgtccattt + ctagcgttccacaagcctcctcatttagcggtagttctattttgagctccaattccagca + ctcttgctgcctcgaacaacgttcctgaatcaactgcaagcggaagttcacaataccagg + actggtcaagctcttctcttccgctgtcacaaaccacttgggttgtcatcaacacaacta + atacacaagggtctgtaacgtcaaccacatccccggcttatgtttctacggccaccaaaa + cggttgacggggtgatcaccgaatatgttacatggtgtcctctaacacaaaccaaatcac + aagcaattgggatcagttcatccacgattagcgccacacaaacctctaaaccatcttcaa + tattaacattggggatatcgaccttacaattgtctgatgccacatttaaggggactgaaa + ctataaacacccatctcatgaccgaaagtacttcaatcacagagcccacctattttagtg + gcacatcggatagtttttatttgtgcaccagtgaagttaatcttgcgtcttccttatctt + cttatccaaatttttcatcttcagaaggctctacggcgaccattactaactctaccgtta + catttggatcgaccagcaagtatccatctactagtgtatctaacccaacagaagccagcc + aacatgtgagctctagtgtgaactcactcactgattttacttcaaattcaaccgaaacca + tcgcagttatatctaatattcacaaaacttcgtcaaataaagactattcattgacgacta + cgcaattaaagaccagcggaaagcaaacgcttgtgctttctactgtcacaacaacggtga + acggtgctgctacggaatacacaacgtggtgcccggcatcaagtattgcttatacgacat + ccatatcatataaaacattagttttgaccactgaagtctgctctcattctgagtgtactc + caacggttattaccagtgttactgcaacaagctctacaatcccccttttatcaacctcta + gctctacggtattatcttctacagtatccgaaggtgcaaaaaatcccgctgcttctgaag + taactattaatacccaagtttctgctacttccgaagctactagtactagcactcaagtgt + ctgctacttctgcgacggccactgctagcgagagttcaaccacatcccaggtttctactg + cttccgaaactattagcactctcggtactcaaaactttaccactactggaagcttacttt + tcccggctttgtctactgaaatgataaatactactgtggtttcccgaaaaaccctaatta + ttagtacagaggtatgttcccattccaaatgtgtcccaacagtcattaccgaggttgtta + cttcgaaaggcacgccttctaatggacattcttctcaaactctacaaacggaggcagtag + aggtgacattgtcatcccatcaaaccgtaactatgagtaccgaagtatgttctaattcga + tttgcacaccgactgttattacatctgtgcaaatgagaagtactccttttccatacttaa + cttcttcaacgtcaagttcctctttagcctccaccaaaaaaagttccttagaagcctcct + cagaaatgtccaccttttctgtcagtacgcaaagtttgcctttggcattcacttgttcag + aaaaacgctccaccacatctgtctctcaatggtcaaataccgttttaactaatacaataa + tgtcctcttcttctaatgtcatatcaacaaatgaaaagcccagtagtactacctctccat + acaacttctcttcggggtactctttaccttcttcttctacaccttcccaatattcactat + ctacagctactacaacaatcaacggaatcaaaactgtgtacacaacttggtgtccattgg + cagaaaaatctactgtagctgcttcttctcaatcttcccgcagtgttgacaggtttgttt + cgtcgtcaaaaccatcctcatctttatctcagacctctattcaatatacattatctactg + ctaccaccaccataagtggtttgaagactgtatacacgacttggtgtccattaacaagta + aatcgactttaggtgctactactcaaacttcctcgacagccaaagttagaattacttccg + cttcatctgcaacatctacttctatttctttgagcacttcaacagaatcagaatcttcat + ctggatatttgtcgaaaggagtatgctcaggtactgaatgtacgcaagatgtgccaacac + aatcatcctcacctgcttcaacgttagcatattccccctctgtttctacatcatcatcat + catcattctcaacaacaactgcatcaacactaacgtcaacacacacctctgtcccgttat + taccatcatctagctctatatcagcatcttcgccatcatcaacttcgttgttatccactt + ctttaccatctcccgcttttacgtcatcaacacttccaacagcaacagcagtatcttcct + ccactttcatagcgtcttctctaccattgtcctctaaatcatcattgtcgttatcgccag + tctcgtcgtctattttgatgtctcagttttcatcatcatcatcatcatcatcatcattgg + catcgttgccatctctttctatatcaccaactgttgacactgtttctgttctacaaccaa + ctacttccatcgcaacactaacttgcacagactcacaatgccaacaggaggtatccacta + tctgtaatggatccaactgtgacgatgtgacttcaactgccactactcctccatctacgg + ttactgatactatgacatgtactggatctgagtgccagaaaaccacatctagcagctgtg + atggttactcgtgtaaagtatccgaaacgtataaatcaagcgctacaatatctgcatgta + gtggagaaggatgccaagcttccgctacaagtgagctaaattctcaatacgtcacgatga + cgtctgtcattaccccaagtgccataacaacaacatcagtggaagtgcattcaactgaat + ccactatatcaattactacagtgaagccagttacatatacatccagtgatactaatggag + aactgataaccataacaagttccagccaaactgtaattccatcagtaacgacgataataa + cgagaacaaaagtggccataacttcagcaccaaagccaacaactacgacctatgtcgagc + aacgactttcctccagtgggattgctacttcttttgttgctgctgcatcctcaacttgga + ttactacacccattgtcagtacgtatgctggttcggcgtcaaaatttctctgtagtaagt + tctttatgataatggtaatggtgatcaacttcatttaatttcccgtatatattatatgta + cgtatatatgctataaacagacactctttttacttacctttaataatgcttgatgagaaa + tcagtcattgttacttcttccccaaggttgtcatcataatcataccattcattatcctct + agttggcatgacattactttccccgaacttagagaaacttcaaacgttatggtgtcggga + taaaatttgattaattcacaacctctgcaatcaaggcttaaaggaatgaaagaatcttct + ttgaccttcgataaaccatgcttcttccgcacatcttttatcttggcccatttgtcgtca + ctctgatcagttaaatattcatcctcaaaagcgcacaggttaactgacaattcttttgaa + caaaatttacacttcatcaagaaagatgcggtaccttttgatgccggcatagcatattct + tcaaatgtattgatgatgactttagaatcgtgtaattctctacaactggtacattgtaga + tcgaaggcaaattctgcccttgattcgtttgtattctcaattgaaaccttggtcacgttt + tctgataatgttgctttcagaaccaaaaataacggcattatcgtacacaacctggttttc + tttattggttaagttgtctttattcaaaatttttcattacttcttataaacagcttcttg + acgcttgtaatttcataagtttttgatacaagcaatggaaacaaaattaagaaaatgtag + gcaacgaagaactaaatttgaaaaaaaaaaaaaaaaaaatttactgccaagatcatctaa + attatcttatagtagcaaagcttgagcataaaaggatcaatcttttagttttttatggca + aagtacatttgcagtaagtaattagtgatgagataaacaaagaataataatccaattcga + aatcaaaaattcaaacaacaaaaaacataatttcacatatatatctaattcattcacgaa + aaacacgtcagttccttctgtctgttgtaaataggatgcatccgcagtgaatcatggaaa + tatttcaaaactttcagcccaatcattttatttggtcaatgaagccataaacgctccgta + attctaagattaaaaaaaattcataaaaataaggaccaatataaatatatatatttaatg + tgctagtgacaatcctagtgataaaattgcaaattatactacaattggtcatggtgaagc + ctaaacacaccattacgtcttcacagtgttattcattttattttcaaaggtatgctacta + ctaatgcaccagtgtataccatgatacggtgaaaagggcatgtaatattgcgttaatttg + tcgataacctttctttacgtttaaattttttttttataactggcgacactaaacttctcg + ataaaatacaacataaaaagcagtacctagctaagcgaacaacagataataaatagctaa + acatctaggttaatagccaattagcaatacatttaaggattgatagtgtggtatttacaa + ggtaatagcccgctaggctataaggaaactactctaaattcaccttccatatatgtgact + gccaagaaaatattactagttatcaagctttttttgtgtgcattcaacttcaacgaatcc + ttcgtcatcaatgaaacaaaagaacaagccagaactgaaaaaaaaaaatcaaaaccacta + agaatgtcacatccttccaaaactcctgctggctcttcaactcttcaaagaatatcaaga + taaatatcattatctccagtgtggccgacccatgcatgagtgcgaggagcattaagttgg + atcctgggaaaacatgtcaaggacattcaataaagtacttttcatactacaacagacgct + agaagaactagaatgtaaccgtgaactgcctctcaaacacataattatgatagcgccgtc + ttcggcatcctttttcggaaatatactttcctttttccctctttcagctatattgttcaa + ggagtaacataagacataataaccccagtttttttagagaaagttcttcttctttaatta + tagtatgtttgaaacgtacaagaattcattgagtgccctaaggattactgcaaacaaggt + tcaacagtgttggtacacatgactcagcaagaataccgttccccctcacaacgcttatcc + aaggggaggagcatgtcgctacccaaaatatttgctcgtaatttgagatctctgcaaaac + aatgcacctcctggcaaaaacatcaatgtcaattgtttgaacgtcaattcttgttcgttg + tccgcaagcccaagctcacaaattaatatggcttgtaatggaaacaagcaagatcttccc + ataccgtttcccctgcatgtagaatgcaacgatagctggtcaagctccaaacttaacaag + ttcaaatcaatgtttaatcataacagatcaaagagcagtggtactacagatgcgtcaact + tcagaaaaaggtacgcataagcgtgaaccccggtcgacgatacatacagagctgttacaa + agttccattatcggtgagccaaatgtccatagtactacaagtagcacacttatacccaat + gaggcgatatgctccacacctaatgagatctcaggtagctcttctccggacgcggagtta + tttacctttgacatgcccacagacccgtcatccttccacactcctagctccccaagttat + atagcaaaggacagtagaaacctgagtaatggatctttgaatgatattaacgaaaatgaa + gagctccaaaatttccatagaaaaatcagcgaaaatggcagtgcctcccccctggctaac + ttgtcattatccaattcaccaattgattccccaaggaaaaatagcgaaaccagaaaggat + caaatacctatgaacataacaccacgtttaaggagggccgcttccgaaccgttcaatacg + gcaaaggatgggttaatgcgggaagattacattgccttgaaacaacctccaagcttggga + gatattgtagaaccgaggagatctcgtcgtttaagaaccaagtcattcggtaacaagttc + caagacattactgtcgaacctcaatccttcgaaaaaattagactacttggccaaggtgac + gtaggtaaagtgtatttagtgagggaacgcgataccaaccagatattcgccctgaaagtt + ttgaataaacatgagatgatcaagaggaagaaaattaaacgagtactcactgaacaggaa + attctcgcgacaagtgatcatccatttattgtgacactgtatcattcctttcaaaccaaa + gactatttgtatctctgtatggaatactgcatgggaggggaattctttagagccttacaa + acaagaaaaagtaaatgcattgcagaagaagatgcgaagttttacgccagtgaagtagta + gcagctttggaatatttacacctactgggcttcatatacagagatttgaaacccgaaaac + atattactgcatcaatctggtcatgtcatgctttctgactttgatttatccatccaagca + acgggatcaaaaaaacccaccatgaaagactctacgtatttagatacaaaaatttgttca + gatggattcagaactaattcctttgttggtactgaagagtatttagctccagaagtaatc + agagggaatggccacactgcagcagtagactggtggactttaggaatattgatttacgag + atgctatttggctgtactccatttaaaggagataattcaaatgaaacattctctaacatt + ttaaccaaggacgtcaaatttccacatgataaggaagtttcgaagaattgtaaagacctg + ataaagaaactactaaacaaaaacgaggcaaaaaggcttggttccaaatcaggagctgca + gacataaagagacatcccttcttcaaaaaagttcagtggtcgttcttaagaaaccaagac + ccccctctaatacctgcattaaatgataacggctgcgaacttccttttatattgtcttgc + aataaacacccgaaaaggaactcagtgagtgaacaggaaaccaaaatgttctgtgagaaa + gttgcaaacgatgatgaaattgatgaggctgatccattccatgattttaattctatgagt + ttaacgaagaaagatcacaatatcttaacctactctgaaaattatactacggaaaaattc + tatacaaagcaacttgtacaaggccaaggcataacagctcacatagaagtttctttaaag + acatcatacctgaactataacatgtttacagaaagataaacccatggccaaatttctgta + ttgtttctctcaatttatcttatgataaaaatgtaaaaaattatttaaaagtaaacaaat + aaacaaaggaacaattcaaaaacgagaaagtgaatgaaaaatataatatcatttattgtc + tgataatgctgcatttagaacatacgtaccatccgcatcagtggatatccaatgatagta + atttcgcgagtttatccgttgctgttatattatcactttttaatattcttttcaaagagc + tgaataatttcaacgcgtcttcgttaatggattccttccgcaattcttctgaaatactga + atgcccgatttataatatctttgtccaggcgtgccaatttcgccacattcatcccataac + tattataagtcaatcccttttttaacttatatagaaaaattacactcatccagtcctcgc + cagttttttgttcttccacgtaatccatatgataattccttattaacggagatttgattt + ctcccagcatgggaaaatgggtagtaaataatatcaaggggcagtcacttaactcagaaa + aatactttattaaagcataagaaattgctataccatcgtgcgtgccagtacctcttccca + cttcgtctaataatagtaaagaccgtttattgcaatttttcaagatgtgtaggatatcaa + gcatttccactttaaaagtagaatcaccgtttataatatcatcgtgcgcaccgattcgag + tgagtacgttttcaaatatgcttaatctgatctcttctgcggggacaaatgagccgatct + gtgccattatagtaagcagtgccacttgtctaatataagatgatttcccacccatattcg + gccccgttataatattgattttaccgttttctggggacatcatgatatcatttggtacat + aatgaacatccagcgactcgataattggatttcttgcattttttgcgattatggcttgtt + gaccattcacaaaagttggtctaacataatttacgttgcatgatgtggctgctaacgaca + aaatacagtcatactgcgccaaattgagtgtaattttacggagctctgtatattctgccg + taattttgttcaagaattctttatactgtagttcagattcccgaattaataagtccttgt + aatattctagcttttgagtcagtttctgggttctgggagtggtaaatctactgaccatct + tcgtattgttaacttttatccaatcatctggcaagtccttaatttgcgagtttttcactt + cgattaagtaatcaacttcatctctaaaatttagatatggacgtttgagatattttcgta + tttctgccaattcttcctttaactgtgaccgtactgattcgctttccctttgaattttta + ttataccctctgaacaatcatagttatttaaattaaaaaaatccattacttgcttatctg + aatttttttccattaccgcagaaacgttgatcatggtcaaaaaatgaggcaactgagtgg + tagaaagtagttcattcaattcactaaatagtctaaaaagtaaaggagattgtttgccta + tccttccatctgatgacttgaaatgttctgacaggtaagattgatgcatcttgaagtgat + caacgaaagaagttatttgctttaaatagaaatagacttcttttctagaagttgtaccat + acattatgcgatttaaagttcttaataagtcaggggtatgattcaacatttgattcaacg + attcaaaaaatatactgttgttgatttcggatgtaatgcactcaatggcatcaagccgct + cttcaatttggtgtacatcaaccaaaggtttgagaatccattctctcaacattcttaatc + caaacgatgtccttgtatggtccaataaccaaaacaaagaacctttaccaccatcatggg + taaaaatgtccaaactttgcagggagttaggatcaagtaacatatgtatttttgatgcga + agggcgaatagatagaaggaatcaacatcacctgctcattattgtactcaaccatatgcg + aatataacttagaaactaagcgtatgagagacggcgagagttgaattttctcattcatta + cttttattgcctgaactacatgatcttccaaatcatactcctgcttgtgtattaaaggac + atgaaatatctttgaaaaatttcgctacatgtaatggaagatcatctgtatttaccagta + cttctatgggctgtaaatatttgattcgtatctgtagtttctcatcagcaagattaggct + cttcaaattcatcatacacgacttccccgttatttaaattgacagaaattaaggaatatt + tagccacctttccctgatgtacgtcacgggacaaagcccatatactgtttgtatcaccga + gaatacgtttccccctaaggacaaaggtggaattaacaccaaatgtagctttggtaaaga + catttgaaatctttctttcaaaaacgcttgatttgctggcacctggatcatgcttcttaa + tagcgcttgtttctgcttgctctaccacggcaacctttaaattatgatgcacaagtctct + ctaggtgaacgtttaatctgacatccgggaaagaacagtacgcaaactgcctatgattgc + aatcttgaggattagactcatcgatagtcaattttccaggcacaagtttgatgtgaagta + ttctgctaaccgttactgcatcctctgcaaaacatttgtacttgtagcctactctaataa + caagcactttatctctatgatgcattttcaggtccttcacctgtttgtccaagggagtaa + gtttcgctgtaggggactttctggcttttttttttacgaaatcttcctcaccctcttcct + catcatcttcagcctcaacattttcatcacttcgtcttttcataatcctatccaatttct + tagcaaagtcaatatcattaaatgttgtcgaagtaccactagtcttttctgaatttttcg + aattcttggaagaaacactgcctttgagtggaaagctatcattagttactgttgtgcttg + caacagaagataaattgtcctcttcatcagtgtcaaggcagatggattcgctaccagcgc + catttccaaccgcaacttcttgttcttgcttatgcgtcagctctgattttaccgccttct + tgaaaaacctgcttattgtgggttgtcccgccattattgttaatagattcaacaaaataa + atctatttgcactgcttttggctctcaaaagtaccagtttaggttcattacctatcacca + ttgttccagcttatttatcattgaaaattcacgtgatcgcaatattccgtcaggagtgat + aaatacacgaaaatttttcttttttttcggtggcaaccaaagtcaaaccatcgaattcga + ataagataaacaggggcaaaggtgagtcggggaaacactattggtaaaagataagcaatt + gagaaacgtcactcagggacaattgattacatgctatcggccacataccgtgatttgaac + acagcatctaatttagaaacatcaaaggaaaaacaggccgctcaaatcgtcattgcacaa + attagtttattattcacgactcttaacaacgacaattttgaatccgtggaaagagaaatt + agacatattttagacaggtcgtccgtagatatttacataaaagtttgggaacgattatta + accttaagttctcgggatattttacaagcgggaaaatttttacttcaagaaaatctacta + cacagactactattagaatttgcgaaggatttaccgaagaaaagcacagaccttattgag + cttttgaaagaacgaaccttcaataaccaggagtttcaaaaacaaacaggaattacatta + tcacttttcattgatctatttgataaatctgcaaacaaggacattatagagtcacttgac + cgctcctctcagattaacgatttcaagacaattaagatgaatcatacaaattatttaagg + aatttttttcttcaaaccacaccagaaacactagagtccaatctacgcgacttattgcat + tccttggaaggtgaaagtctaaatgacttattagctcttttactgtccgaaatactttca + cctgggtctcagaatttacaaaatgatcccacacggagttggttgacacctccgatggtt + ttagacgcaacgaaccgtgggaacgttatagcaagatctataagttctctgcaagccaac + cagataaattggaatcgtgtgtttaatttaatgtcaacaaagtatttcttgagcgcacca + ttgatgcctactacagcatctttgagttgcttatttgcagcattgcacgatggtccagtt + attgatgaatttttcagttgcgactggaaagttattttcaaactagatttggccattcaa + cttcataagtggtcggtacagaatggttgctttgacttattaaatgcagaaggtaccagg + aaagtttctgaaaccatcccaaacacaaagcaatctttactctacttattatccattgca + tcattgaatttagaattgttcctacaaagggaggaattgtctgatggtcctatgctagct + tattttcaagagtgcttctttgaagatttcaactacgcccctgaatatcttattttagca + ttagtcaaagaaatgaagcggttcgttttattgatagaaaacaggacagtcatagacgaa + atacttattaccttattgattcaagtgcataataaatcaccgtcatcgttcaaggacgtt + atttctacaataaccgatgattctaaaatcgtagatgcagcaaaaatcataatcaactcg + gatgacgcacctattgccaactttttaaaatcgttgttagatacgggaagattagatacg + gtcattaataaacttcctttcaatgaagcttttaaaattttgccatgcgcaagacaaatt + ggttgggaggggttcgatactttcttaaaaacaaaagtttctccatctaatgtcgatgta + gtgctggaatcactagaggttcaaacgaaaatgactgatacaaacactccatttaggtca + ttaaagacatttgacttattcgcttttcattcattaattgaagtactgaacaaatgccca + ctagatgttctccaattacaaaggtttgaatccttggaattttccttattaattgcattt + cctagattgatcaattttggttttggacacgatgaagctattttagccaatggtgacatc + gcagggattaataatgatattgaaaaggagatgcagaactatttacagaaaatgtatagt + ggtgagttagccattaaagatgtaatcgaacttctgagaaggttaagagatagcgacttg + ccaagggaccaggaagtcttcacatgtattacccatgccgttatagcagaatcgacattc + ttccaagattatccattggatgcattggctactacatctgttctttttggatccatgatt + ctctttcaactgttacgtggattcgtattagacgtcgcatttaggataatcatgaggttt + gccaaggagcctccagagtccaagatgtttaagtttgctgtacaagctatttatgcattt + aggatacgtttggccgaatatccacagtattgtaaggacctcttgagagatgttccggct + ttgaagtctcaggctcaagtttaccaatctatcgtcgaagctgctaccctagcaaatgct + ccaaaggaaaggtcaagacccgtccaggaaatgatcccattaaaattttttgctgtagat + gaagtttcatgtcagatcaatcaagaaggtgctcctaaagatgtcgtagaaaaagttctt + tttgttctcaacaacgttactctggctaacttgaataataaggttgatgaattgaaaaaa + agtttgacaccaaattatttttcttggttttccacatatttagttacgcaaagggctaaa + acagaacctaactatcatgatctttatagcaaggttatagttgctatggggtcagggttg + ctacatcagttcatggtcaacgttactttgagacaattatttgtcctactatctacaaaa + gacgagcaagccatcgataaaaagcacctaaagaatttggcttcatggttaggatgtatc + acattagctttgaataaaccaattaaacacaagaatatcgcattcagggaaatgttaatc + gaagcttataaggaaaatagacttgaaatagttgtgccttttgtaacaaagattttacaa + agggcttctgaatcaaaaattttcaagcctccaaatccctggactgttggcatattaaag + ctgttgattgagttgaacgaaaaagcaaactggaaattaagtttgactttcgaagttgag + gttttattaaaatcttttaatttgaccaccaaatctctcaagccctcgaatttcatcaat + actccggaagttatagaaactttatccggtgctttgggatcaatcactctggagcaacaa + caaacagagcaacaaaggcaaattatactaatgcaacaacaccagcaacagatgctaata + tatcaacagagacaacaacaacaacaacaaaggcaacaacaacaacaacatcatattagt + gcaaatacaatcgcagaccaacaagcggcatttggcggcgagggttcaatttcacacgac + aatccttttaacaacttacttggttctactatttttgtaacccaccctgacttgaagagg + gtatttcaaatggctttagccaagtcagttcgcgaaattttgttggaagtagtcgaaaag + tcatcaggaattgctgttgttacgacgacaaaaataatacttaaagactttgccactgaa + gttgatgagtctaagttgaagacggctgcaatcattatggtaaggcatttggcacaaagt + ttagctcgagctacttcaattgaaccattgaaagaaggcatacgttctactatgcaatca + ctagcaccgaatttaatgtctctttcttcttcacctgcagaggagcttgacacggcaata + aatgaaaatattggcattgctctagttttgattgagaaagcatctatggacaagtctact + caagatttagcagaccaattgatgcaagcgattgctattcgtcgttatcacaaggaaaga + agggcagaccaaccatttattacgcaaaataccaatccatattcactgtctttaccagaa + cctcttggtttgaaaaacactggtgttactcctcaacaattcagggtatacgaagaattt + ggtaagaatattccaaacttggatgttattccgtttgcaggattgcccgctcacgctcca + ccgatgactcaaaatgtgggttcaactcagcctcagcaacaacaagcgcaaatgcctacc + caaatcctaacctccgaacaaataagagctcaacaacaacagcagcaattacagaaaagc + cgtttgaatcagccatcccagtcggctcaacctccaggagtgaatgtcccaaatcctcaa + ggtgggattgctgcagttcaatcagatttggaacagaatcaacgtgttctcgttcacctc + atggacattttagtttctcaaattaaagaaaatgctacgaagaataacttagctgaatta + ggcgatcaaaaccaaattaaaaccatcatttttcaaattttgacattcattgcaaaaagc + gcacaaaaggatcaattagctttaaaggtatcccaagctgtcgttaatagcctttttgcc + actagtgagagtcctctctgcagagaagttttgtccctacttttggaaaagttatgttct + ttatccctcgttgctagaaaagacgttgtctggtggttagtttatgccttggacagtagg + aaattcaatgttcccgttatcagatcccttctagaagttaatttaattgatgctacagaa + ttagataacgttttagttactgcaatgaaaaataaaatggagaactcaactgaatttgct + atgaaattaattcagaatactgtcttgtctgatgatccaattttgatgagaatggacttc + attaaaaccttagaacacttggcctcttcggaagatgaaaatgtaaagaaattcatcaaa + gagttcgaagatactaagataatgccagtgaggaaaggtaccaaaaccacaagaacagaa + aagctttacttagtatttacggaatgggtaaaattacttcaaagagttgagaataacgac + gtaatcacaactgtttttatcaagcaattagtcgaaaagggtgttatcagcgatactgat + aatttacttacatttgtcaaaagttctcttgagctatcagtttcttcattcaaagaaagt + gacccgactgatgaggttttcatcgctattgatgctctaggatcgctaattataaaattg + ttgattttacagggtttcaaagatgatacaagaagagattacataaatgcaatattttct + gtgatcgttttagtgtttgctaaggatcatagccaagagggtaccacattcaatgaacga + ccatatttcagactattttctaacatcttatacgaatgggctaccatcaggacgcacaat + tttgttagaatatctgattccagcactaggcaggagctgatcgaatttgattctgtattt + tacaacactttctcaggatatttgcacgctctgcaaccatttgccttccctggattctca + tttgcatgggtgacactattatcacacagaatgttattaccaattatgctaagattaccc + aataaaataggttgggaaaagttaatgcttttgattatcgatttgtttaaatttttggac + caatacacaagtaaacatgcagtctctgacgctgtttcggttgtttataagggaacactg + cgtgttattttaggcatttcgaatgatatgccatcctttttgattgaaaatcactatgaa + ttaatgaacaatctacctccaacatatttccaactaaagaatgttattttatctgctatt + cctaagaatatgaccgttcccaacccatatgacgtggatcttaatatggaggatattcca + gcatgtaaagaactacctgaagtcttctttgatcctgtaattgatttacactcattgaaa + aagccagttgacaactacctacgtattccctcaaattcattattaagaacaatactaagc + gctatttacaaggatacctatgacataaaaaagggcgtaggctacgactttttatctgtt + gatagtaaattaattcgcgctattgtattacatgtgggcattgaagctggaatagagtat + aagagaacttcttcaaatgcggtatttaatacgaagtcttcttattatactttattgttc + aatctgattcaaaatggtagcatcgaaatgaaatatcaaattattctgtctattgtggaa + caattgcggtatccaaacatccacacctattggttcagctttgtgttaatgaatatgttc + aaaagtgacgaatggaatgatcaaaaacttgaagtccaagaaattattttaagaaacttt + ttaaaaagaattattgttaacaaaccacatacctggggtgtttcagttttctttactcag + ttgataaacaataacgatattaatcttttagacctgccctttgtacaaagtgttcccgaa + attaaactaattttacaacaattagtaaaatattccaaaaaatacacaaccagtgaacaa + gatgaccaatccgccaccatcaatagaaggcaaacccctctacaatccaacgcataaaaa + aactgcatttatttatatacgaaaatcatgatttcagaaaaaaaaatataacttgtataa + tactatatttcagagaatactattttgaataagaatagaagcaatttcgattaacttttc + ttactgtatatgggtcgctttcacttttgaactactgaaaattagtgttacccgtattat + tgttttggagaataataataataatgagcatcaatttatatcaaacaagagctgcaatta + ccttatgtccatgacgcactgcgaacaaagaataattgagtaatcgaacttgaagttcta + tagaacagcttgtatatggtttcattgttcaaaagaggtaaggctccaccgctcacgaaa + gaaggccccacttctaaaaagcctcctaacacagcgtttagacaacaaaggcttaaggca + tggcaaccaatactgtctcctcaaagtgtgcttccgttgttaatattcgttgcatgtata + tttactcctattggtattggactcattgtaagcgctactaaggtacaagatctaacaatt + gattatagtcattgtgatacaaaagcatctacaactgcttttgaagatataccaaagaag + tacattaaatatcactttaaaagtaaagttgaaaataaaccacaatggaggctaaccgaa + aatgaaaatggcgaacaatcatgcgaactgcagttcgaaatcccaaacgatatcaagaaa + tccatttttatatattataaaataaccaatttttatcaaaatcatcgcagatatgtccaa + tcgtttgacacaaagcaaatattaggggagcctatcaaaaaagatgatctggatacaagc + tgtagtccaataagaagtagggaagacaaaataatatatccctgtgggttgatcgctaat + tccatgtttaatgatacattttctcaggtgttgagtggtatagatgacacagaagactat + aatttaactaacaagcatatatcatggagtattgatcgtcacagatttaaaaccaccaag + tataatgctagcgatattgttccaccgccaaactggatgaagaagtatcccgatgggtat + acagatgaaaatcttcctgatatccatacttgggaagagttccaggtatggatgaggact + gcagcctttcccaagttttacaagttgacgttgaaaaatgaatctgcttctttaccgaag + ggtaaatatcaaatgaacattgagttgaattatccgatttcactctttggtggcacaaaa + tcatttgtactgactacaaatggagctattggtggtagaaatatgtcactaggcgtactg + tacctcatcgttgcagggctttgcgccttatttggcatcatttttttggttaaattaatc + ttccaaccaagagcgatgggtgatcacacttatttgaattttgatgatgaagaaaacgag + gattatgaggatgtacacgcagagaatacaacattgagggaaattttatagaggccaaaa + aacaaactttagtgcctgtaggtatttgtgtttataagtaaacaagttttgttgacttcc + tgaaacgtaaacaaaagaaaaattgaagtatgcctttttacgcttggaaaatagtgcgac + aaagaactcccagggtttgctattcccatttagatgatgaaatgatagaaaagtggaaaa + cgtttatcatgaaatcatctatacatatacgtaaaaatgtaaactatgtaaaaatatgtt + ccctataaaagtgatgctcttccggaacgttttctttttcccacaaatctcgttgaaacc + tgaaccaatctggtaattcagaatcacagggtatccttattttgacgttatcttttcccc + ttgctttataatagccataatctccattttcttttttattaaatgctaatctatacctat + ggccaaaaattcctaggtcagaagaggttttcttcaaatcgtctcctagaactccactta + cttgtaatactttattatttttcactttggtttcgatttgatttattaatgtcaaaagcc + gctgaggcacttctggctctctgcaaaggtcatcttcatttactctaataagtttgccag + aattggccttatactctatcgaatgcgtcctattattctcaaggggcaatttttttgctt + tgttgtccacaattgtcttattatccttctcctgttctatctcaatgttaattatttcta + gaaatgtttccgcaaaataatttctattcttaccagaaaataatctgtactcatttatga + ttgatgcaatattccatttctgaatttttcgaagtataccaatgaccaacgcggtcttat + ctaccaataaaacattataattatccacattcaataatgttttgaaagttctttttaaac + acgtgctcttaatcagcattaagtcatcattgtcagtcaagtggtaagccaattcctcct + gaattacaggatcccctgtagtagaattttgtgacccatttgcagtggattttttctttt + cttcctgtaatgatagagtactgttattattcgaatataaatttgcgttagatacagaag + agctttttaccggaactgccgcagccgagaaatcagacatccttaaaacaatccatttta + tggatgatcttgtgaaaaaatctttgaaaaacttggatggttcttgacctccaataaata + tagctgtctttaaattgagagtttccagaaaggataaatttaatgtctccactttagaac + atctatatattccttcttcagcaatcccaaagttagcaggtggaaccagcattatgaatt + caggttatggatcgcaccagtgctgtaagtgtctctctttttattctacgatctccatat + aacaaccacatctcatttctttaccttactgtccatttaatgctagaaaacttccgaata + ttgtattattattacccggatatgtcaaggcggtatattaagatgttcctttcaaaagtt + tcattataccgcatacggtaaggacagccataagtagttttactgataaaatatggggct + aaagaactgtatatttatggatagcactaaaagtaaagattttccccgtgtcctgacttg + gagaaacaaaatttcattgtcataaaaaacttttaaaatatgatattcaaagcttgagca + ttgggcttctggatgacagttctaatcaagttgggtctaaggatattgcatgtatacaaa + ggattctttcggaaagtaatcctgaaatatttttttttttcgtctgaacatacgaaagta + aataaaaaaagttcaatgcatgccttcctatgcaagatttataaaaggtgaacaggtcag + gagcagccacatctcaataaaggcagttaaagacggtgatgtaggtgcagaaagagaaag + tgaacaaaggatgatttgagatgtgtaatgattcgtttgagcagcaacaactgctaagat + attcatactaataggccacttcacacattatgccttggagaatatcctgcattgctcctt + ttatagtatagtctattcagcactaacaagcaaagcgaacataatggtaaaactaacttc + aactgttgctggtgtcgcctgcatcgctggccctcccctccactacggccattttagcac + aacttgaatgagtcaacttggttgagttgcatgttaacgtctctgatatcagagctcact + tgctcaataatacatgttacaaactgcccactaaattgaacactctaacattatattgac + ctgaattatttgaaacgaagtcaacagaatgatcactgattttccaatgtactccgctat + gttgaagctagccatcttaagcgatcaatgacggtgctcaatcattcttcgaaatgggct + aaaaatgacagttgaaatatattctttacgttcattttttttcatactgcaaacccattc + tagaaccaatggtaaaagtgacgggagttgctttcttttactatttcaatgatagctata + gcctgcatacacacaaaagaatgtaagtgattgcgtgaaagcttaaagtcttaaaactgt + atttttttcaactcctcccatatattaaactggtcctcacagttcgcaggataactaaca + attaaaaaaattgttcatatggttgtatttctttccgtctcacattattaaaattcaacg + aagaagtttctacaataacagacaagggcctacgattactatgtactggaaggtgagtct + tgaattcatcctcagagattgttctaaagaccaacagaaatccatatagattaccgtagt + tactctgatggaatttcagagtaagacccttgtcaacttcatcactttaagttgagatgt + agaaagacacttttgtaaggtctccaacaagtatatttgataaatagactgttgagctac + aaaaactcaatgtgaagtaaaagacctcataaatgatcgattatctgtttggcaggtact + aataaaactgcattggtgtcttttttcacttacgttaaaaaaaactttttgcaatgattt + aagcgtgcgtgaagataacactacaatccattttaaagcaacatccacattgagtgtata + caccacaaaggttttttcagggcgtttttctcgccactttatgttgaccaaaattattaa + tggaacttacaacgtttccaaaagttagttaaatacatacgtctatttactaagcaagaa + atatatcatgacaagcccaaatattatattgttatgtttacaaaaaaaaaatggctatat + acatcaagtctggaggctttttataacaagcaagtggggtaacttagacataagattgac + ttctttgaattcaacaaaaatacatacttttgatgatttcaatggtagaagcagtagtaa + ctatgccgatgaaataaaatcctgatatcatctatatagtagtactcacgttactagtat + atcatctataagatgtaggaaaatgtcataagaaatgagaaacagtcattaaatttaatg + gaaacgaaatgcaaggattggtgatgagataagataatgaaacatagaaaacgaaaggag + aaataacagtagtattattattattattattatgtagaaatattgactctctcttgtaga + ttcatatatcttcaaggggaacttcttgtacattttgtatacctatattatagcgtttaa + caacaatggaatcccaacaattaccaaaattttcacacattcctcatataaaatctaagc + aatgactagagaactatcgctaaattatatagagcaaggaaaccgaataattacatgtgt + cagataagaaaaaaataaagaaaacaacgcgtcatgaaaaagagttactgaaccttcaga + tcctacttattgtaatgcttcgcgacatccaatccatttaataatcaatttaaaactaga + gttggtagagttccttgttgaacgtgataacccaaaagcataatacgagtaatgtttcag + tattgctattatatgtttacacaaggaaaacatataataacaaacctctaatccggtagt + acttaagaaactatagtttctatgtacaaaaaggtaactatgtaattcttacatttacat + aacatatagaagggtccaataaacttactaaacttactaccttgttgtatataggctaga + tcgtaatccactacgtcaacataaaaaaaacttaaggagtttgaattttatgtacaaaca + gattgttaaaatataatataagattatggaaacgaacttgctctaaaaaaaatttaaagt + tttataaaatcctcgcactatcgctgttatacatgatgtccccaaagcgtgtacaaataa + ttttgtagtattgtatcggtaatatcatacaagaagaaaatagttgcaaatctctaaccc + actagtacttaaaaaaactgtagtttcagtgcaaaaaagttttaacattacgtatcttgt + accctttttattgcatatagaaaggtcaaataatccttcacatcatgaaatataagctaa + atcgcatttcttttcgtccacatttgcaaacaaaacttttcaataataattttataaata + gtatcaatatatatatatatatatatatttatttgtttactttttctatcagtgttttca + attttttattaaacaatgtttgattttttaaatcgcaatttaatacctaaatataaaaaa + tgttattatattgcaaaaacccatcaaccttgaaaaaaagtagaaacgttttatttaatt + ctatcaatacatcataaaatacgaacgatccccgtccaagttatgagcttaatcttccat + aaaaatatttgaaagcaatagatcatgtactaaactaaaatcagggaaattaagactcct + tttgaagtaatacctattacttactaatacgtttgagaataagcgcaggtactcctggtt + tttgttaaaactacaaatttatacttagcattacgaagattctcgattccgaaaaacaaa + aattttatcgtcatatacaaatctagaaattaccagagctatccatcttgttcaagaagg + taggcgaggacattatctatcagtacaaacatcttagtagtgtctgaggagagggttgat + tgtttatgtatttttgcgaaatatatatatatatatattctacacagatatatacatatt + tgtttttcgggctcattctttcttctttgccagaggctcaccgctcaagaggtccgctaa + ttctggagcgattgttattgttttttcttttcttcttctattcgaaacccagtttttgat + ttgaatgcgagataaactggtattcttcattagattctctaggcccttggtatctagata + tgggttctcgatgttctttgcaaaccaactttctagtattcggacattttcttttgtaaa + ccggtgtcctctgtaaggtttagtacttttgtttatcatatcttgagttaccacattaaa + taccaacccatccgccgatttatttttctgtgtaagttgataattacttctatcgttttc + tatgctgcgcatttctttgagtaatacagtaatggtagtagtgagttgagatgttgtttg + caacaacttcttctcctcatcactaatcttacggtttttgttggccctagataagaatcc + taatatatcccttaattcaacttcttcttctgttgttacactctctggtaacttaggtaa + attacagcaaatagaaaagagctttttattcttgatttttgttctttcggggaaactgta + taaaacttccaaaaaggaaaagtaaaacaatacatctccttatatcaaagaaaatcaaga + aggacaacatggatgatatttgtagtatggcggaaaacataaacagaactctgtttaaca + ttctaggtactgagattgatgaaatcaatctcaatactaataatctttataatgtatgtt + ttcatttcaaggatagcctttgaatcaatttactaacaatacttcagtttataatggaaa + gtaatttgactaaagtagagcaacatacattacacaaaaatatttctaacaataggttag + aaatataccaccacattaaaaaagagaagagcccaaagggaaaatcatcaatatcacccc + aagcacgggcatttttagaacaggtttttagaagaaagcaaagccttaattccaaggaaa + aagaagaagttgcaaagaaatgtggcattactccacttcaagtaagagtttgggtatgta + atatgagaatcaaacttaaatatatcctatactaacaatttgtagttcataaataaacgt + atgagatctaaataaattcgttttcaatgattaaaatagcatagtcgggtttttctttta + gtttcagctttccgcaacagtaaaattttataaaccctggttttggttttgtagagtggt + tgacgaataattatgctgaagtacgtggtgacggatattgggaagatgtgtttgtacatt + tggccttatagagtgtggtcgtggcggaggttgtttatctttcgagtactgaatgttgtc + agtatagctatcctatttgaaactccccatcgtcttgctcttgttctcaatgtttgttta + tatactcatatttctatgtgtttatacaattgctattgtttatataatgtagtgacattt + tctcttaatcttatactaatttctatgacatttatataagaagagacttatgatcaacat + aattttgcaaactttgagagaaatatgtctttctactgcgataaagttattatttagatt + acatgtcaccaacattttcgtatatggcgatataatttatcatgttttggtatgataatt + taatttttaaaaaaacaaatttaattgacctcattaattaatatttattaatacctttaa + atgttgaggtaaatagctattttctctcttcttttcctttagttggaatttgcacaagaa + aatgtttttccacacactttagcgttttttcctaaatgttggaataaaaaacaactatca + tctatcaactagtagtcacactaccaatgtgttatcattatactgtgttaaacaatgaca + taaggtatgaaaatttgtcaacgaagttagagaaagctggatgcaaggattgataatgtg + gtaggaaaatgaaacatataacggaatgaggaataatcgtaatatcagtatatagaaata + tagattcccttttgaggattcctatatcctcgaggagaacttctagtatattctatatac + ctaatattattacttttatctacaatgcaaccccacaataatataaaaattcaccaattc + cgcatctgcagattactttcctaaatttgcatatagaattgtcaagcgcaaatccgacgt + cgattccgcggcggatgggtcattctaggtcattctaccaattttatttgagaccaggtt + tattcaaccggtaacatagaaatattcatacaattaagcttctatggccaagttggtaag + gcgccacactagtaatgtggagatcatcggttcaaatccgattggaagcattttttatca + cgttattcggtgacacccaggttgccgccgcgttcgcgtccatcgtcatctgaaaaataa + tgaatattaatggaccttgtgccccataaaggttccatgttccataagtcttcaataata + cttttgtatattaaatttaccgttattcggagatctcttacggcttatgattttctttac + attccaggccgccttttgatgacattcagtgcgtcacgacgcgaatagggctttgaaagc + cttaatgtggagtaaataaatatatgaatgcaataagtaaacataagacgagttcttcta + tatccggtgtacctaataatatagattttgccaacaatggaatctcattacccatcccaa + gataaaatattgatcaaggcatgggccaccaacataggaatacgcaaaataggattgata + tttttacatattatattatctttcttcttctgtctcaaatatagctcttctcctatttaa + aaaattatcaattcttgaatctcaacttccattaaatttgatgattgtttcccaatatcg + atgtcatctttttacactgtaaattataatttactaggaacatgaatactgatcaatgga + tgatagttgattcttattgcaataaaaagtactttctaagacgctaggacttctaaacac + agatattatgaaaatgccaaaaaatacagaatattttccttcttttcactataacaagta + gtaagaaattaagcaatatggtaatttgagcttatcagataaaagatcctggtagtcttg + caaaggtgtgagtacttttgatgaaactaagtttcagcgcagctatttcaattttggaac + ctttaaagaaagtttaatcgaaacattgaacgatccaaagcacaactttgtagcgggaag + tcacaaacaaatataaaaagtgttaataaactgccatcctatgttagaattgtgaaactt + tttggactaataaaaggttttcaacatggtgttccaaagcacttatttctaggttagcta + tggtaacgagttttaacgtaatcaaaacaatatacaattcaagatctgtatcatttacac + taacacaccccattttaatagagctgagtacttcctacatttcttttgcatatgatatcg + ccactgcatcatttctgtagtcgtaaaatttccttctcattaagacaccgaaaatagcta + aggattataacaaaacaaacaaatgctcaaacaaaacttttagtaaatcttttgaaatgt + aaaagttacttagaataactaaaaaaaaaatattacgctataccatatatttccattccc + aattttatcgttttataataatattcagtttcaagaacaatgaatatatttcaaacatta + catgcactcttgggagtaagctgcatcataataaattaaaattaacgctttagaaaaaga + aattttgaggttaaaggatggaggaagaaatcgataccaatttcaactttgatcgacctg + tctgactgaaattatttctgcgtttttcttactacaattagtaccattctcttcaacttc + aatagttctaaccattgattcttcatctgtctcatcaaatcccatcttaccagtccagcc + attggataccaaatagttgtgaaattcaacgtcttgcttcattaaatcgctttcaagaga + atgtggaacaaagaaatatgtaataatgataccaataagaccacaaattgcagcaataat + aaaagtccatcttgcacccaaattatccctaatgggttggaaacattcgacgcctactac + agaaccgattttaccagtcacagcagataaaccatagaaaacacctctaacagcggttgc + tgacgcttcactactaataacaccaagcatatcacctggtccagcattacctaacatatt + catgaatgcgtagaagataataaacaagggggtgatttttttcaattggtcgtacgcaca + tccaatgattagaccaaagatgatgtacccagagaaaccaaacatcaacgtatatttacg + accaatacgatcggacagataagcaccaattggtacacccagtacagctaaaactcccaa + cagtaagttccactctgccacttttactaaatcattttggtccttgataacggaactgat + aattgttgaactgaaaataccatttgggaaggtaacaaaatcatacataaaccatgtacc + acatgtaccaagtaaccttttccaataaaatttcaatgctaggaaatatggtatatttct + cttgattctacctttttcatagacttctgtagtagcagtcttccatctaaaatagaacac + actcaatggccagaagcaccctattgcaaaaacagtcctccagatcgcctctaaatgttt + tgttcctgaacagattttgtagacgattaaaaagatgatcgtagcaaatggaccaccgaa + ggctagtggcaaatttgtcaccataaccaggataccacctcttttggtagtggtatattc + attagcagactcattagcacttaatgtactggtaggatattctgcaccaacaccaatacc + taccaaacctctcataactgttaacatccaaaacatgccaggtacagtagtaccgtgaga + ggcagcacacagagcactaccaataaccaagatagcagtggccacaaggatacaagattt + tctactataataatcagcagcgatacccataaagaattggccaaaaataataccaactag + ggctgcgttggaaactctagtcgacacttttgagctatagtttttcttaccgtactccat + aacaaaaaccttgtttagcatactcattgaaccatttacgtaaccatcacttatcagagc + aaaaccggacgcaatgattgtaactatgtttttccatttatctttctttgaggtttcagt + acgggttgcacgcctctcggcatcatattttattattctaggattagtgttctcgttcac + ttccttctcatttaccgatgtgatatctttgtcttccattttttatcctattctattttt + ttgattataagcaaaaagatcaactcacgtctgcggctggttgactagtcacaagaaaca + gataataaatactgtaacgaacatctgctacaactacattcatcggggtttatatattca + cgagtttcctacttcatccagacacattctggatgtcttccagcagatgtccctcatcaa + agaattaagatagccagattgaaaaactaggttaaccagagaactaagttaactaatgtt + ggaaaataatcctacgatacagtggacggaatgcccatatgcctatataatcaatccatg + ctaaggtgggggtccagtaaggaaattcatgaatatgaagataaatgtggcaccaaacgg + caacgcggaagaccgtaggatccaatcatataacccctattattctaacatcattgctgt + tgggtacaacattctacgccgctattactacgtgctgcttcttttgattctctgctttct + ttaccgtcatgcacgtgcgccgcgaaaagtattaatcatgcggacactctggcatttagg + ggatactgtcaaaaagactatgctcaatactgtcatattcatatgcacgttcttttttta + atttatcttggcaaaacgtggggctggaacttttgaaacgcactgcaacatctgtggctg + tacagtcaagaaaagtaagtactcatcgtcatgctctaatgtgtatatggtatttgtggt + gcagacaccattagaagtatagttcacgaaatgatgacacgatagcatgtgcttttcgac + ggctgataaccttgtattctcttatttttgtattcttttcgtaagcggcatttgtttgca + gaaccttatcgcagatatcgataaacatttgttgacaattctttttcgctccccttcttt + ttgtttatcttcgttatattcaatagtctacatacactaaccgttgaattgttgcaaaca + aatactaataaaaatctatatatagtaaattgaagatgtactgatcagtctatcattgtt + tagtgcacccagactcgaatcttaaataccactttacacacctactaaattttgtcctca + caaaatgaagacaggattcaaaaccgattaatagtagcagaaactaaaaaagtacgaata + ttagtaaaattcatgttcttgaatcgagctactatctttgtcgggagggtaaacgattat + aactcaaaatgactggaactggtgattattaatttttacgtttcctgtgccaataagcgg + aagataagaggatagaagaaaagaaaggcggcacttggcgaactacaatggcgattatat + tcatggcgattatattcatacaaaggtaatggaggcctcggataatggacaatattgaga + aaatccttatgcttacttctcttaataaaaaatagacacagccatttattatgcgtaaaa + aagattacccacttgtcttcgatgcgtgctgctgccaatcaaccttttgagcggaacttc + gagctcgcaatgcgtctggaatgttgctagagacagtcttggttatctgtgacatgtgtt + tcgttcaggcgtgtgagcatcttcttgttcgatttcaaaattaccgccttgactcgtgaa + actggataattcgttggcgttttcatataagtcgtctgatggcgaaaacttttcctttac + ttagcatacagcaaatatccccatttgacggatttttgaaaaatgagcccgctaacccag + aatgaactgcattaccaagcatttatgtaaacgttccgccaccatctttggtaaggtata + ctattatgttctggatttaaggttgattcacaatttttcatcaccaaaatctggtggcat + gcctagttgtctggtttcaggcaatttagccatcatagaaaagcatcctctgtcttgagt + tgagaaaatgttactcatagagccaaacaaataaaccctggaattaaaatatagtttaaa + cacaacttcatattacactgtaaatacatatcacaataataaattaacgaaatagtaaga + ccttatgtttctatatgacatcagtcccgaatagtgtcaattttttgtcgtcagtgtcga + acatttttactgtttattcatcatatcgtgaggtatgtaggcatcttctattgacttaag + catgataaaagattcacccaaaactgtatcgaaatattgataaaattaaatttcaaagtc + aaacttgttttcaatggttgctattttcttaccattcgagctctccctcaggattttttc + acatggaatgttcatggcctcaatttccatgggctttttgcatttatctcctttaattaa + ttgtaatggctctaatgatacagtttcgccattagctttgtcacatatatcggaaagggc + aatctgatcataatctggtatacagtcaccctttgcatctctaacaaattcagaagagca + ttcgtagtcggattcagtacaactgtcacaaggtatttcatgcaaaatcatatctttgaa + tgtcctttttaccaaacaacgggcgtcctgtttcctccttttatacatgtatttggcgcc + attaaggcatttcccatctgccaaattccagtcttcgaaatcttcctcttcacacgtttt + atagtcaaaatgcagcagaaaaatcgatcgcatacaggacacttcttgcaatatatgaga + tgttggtgttgtttccttcttgactagccgtagtaatgagatgtccgcttaaaataaatt + ttgagcctgatccatctggcgttgtattaatcaactctaatggatggatataaaataaag + cgtcaacaagctcatattgattccatgttctaccttggtccaaggaaaaataaagtttgg + attgtacatcaccatctttatatgaaggagacgggatatatacaataatatttcccagat + caccagtagcatataatccagaagaattatgggccaccctccatgttagaccaccgtctc + tagaaatgaaagtttttcggtccttccgatcgaattcattgtcatcgccaacagaacctg + tcattaatatgatcccagcagaaggatttgaaagttttagattgtaaaaataaccctgaa + gtgaacatctctcaggcctagtgatatcacagccgaacgaatcggcattttcttcatcaa + caactttcaaatttgaccatgtgaggccgttatcaacggatattttggtttctcctccgg + ctatattttcagtatagtttacttggtgattagaatagtcaatggaaggatgaaacgatc + caaatattgtccctttcaagaaatcaggaaaagtgagcctaaaatttccaaactgatggt + ttggtgtccattcaacaggtgagaatttcaacccttgcgaatctgatatgaaaatctctg + agagatctggatcagcattttcctcatttgtgatttctgtcgatataagaaggatgatcc + ttccgatagaatcttcacgaattttaatcactttgacgtgccgtaattgagtaggcagct + gtgccttttgaaaagtagatgcatcattggatatccagatgtccgtagatgacatctcgt + tatgcccgtcatcttgcgttaaaacgaccacatatgacttcaatatcttgtattgagtaa + caatcttatctttaaattcatcaaaatctttaaacgttttgcctccatcactactgaaag + ccaactgtgtgtaggtagcagttaagcgatattcttcatcgtattcacggttagagaaca + aacagagtatcgaagtatcattaccagtaaagtcagaatccttgctagacttaatgaaat + gacagctttaagttgttatatcgtcgtcttttttcttttccaaggaaggtttaattttaa + aaaaggattcccatcattgctcaagtaaatttcacttgggtcatataaaacgtctgcgtc + tatcatatagctatagcaattcgcaataagaagttctttgtttctaggatgagtagttat + aaaacagtcgccatctaaatattcttctgatgagggtatagttaaagccctccatgactc + cccacggtcgtcggtaatgtagaatttgggtgattcacatataaaagcgaaacctctttc + ctgtccatggaaaggatcaacagtaaatctgaaaatgcgctcttcaatttctttgaccgt + ttcccaattttctccagcgtcgaaacttatccatacaaccccccaaccatctaatctgat + agaagtgttggaatcatcaaaactcactagattaaatgatgtttctataggagcctccgt + tacttttgggacaaatttctctgcattgagtagcggaagtagtataattacccaaagagt + atatatggcatgaagtaatatcataattgaggatctcttaccttttcttagttcaaagtt + ttttttcaaagctcggaactttatgctacaattcaatgactaaaaaaaaggtagcggata + atcttttggttgcaaattggcggcatttaaatatattttgggttgcaagtattctaaaaa + tattctcataatattctaaaaatatccagtctaggtaattgcaattattttgagtccggt + gactttgagttgaccatgttcacttcctgtacagccatcaaggtaacgtgtttaccatct + aatggagaaaaccaagagataagactagaaacctagttaggatacttggatttgctaatg + tcgccagtagtacgataataccataaagatacgctgttgaacctgatttgtatatttaaa + gaatgggaatctttaagggatcatagtctagaatggtattctgcagaatagattttaaat + ggtgaaaactttccctcactatgtccaattgatgctttttaggatgacgttagaatttta + ttctggtgaaggcacttttaatatgaagattcgttgaagtatcaataaccttttattggc + atacccttacctaactggtctctctcacctcgtgatgttacgaacgccacgattattctc + aaactaggtaaagattttttcttcaaaattagtgggaaagactcgacagacccgcttacc + aggtattctttctaaacaatagggtttaccagtacccctgaaaatgataatgcccatgtg + cggctgatcgcgttaactacgtatgcagttgaatcaaagccataccacagaaaatggtac + aagagcccactggatagaatagtgctggctatcaaaccaatattttctttccagctcttg + ggggacctctactgcgttatatcgtagtttccattcagcctgcttatcgccttaggaaaa + gggaatgtaagaggcagaaaagtgcggcgcaatcgttcggacagcgagccgctattatag + gtaagaattcttaaagagcttcgccggatattattacagtgtattaaatttcgccaagtc + tttgccaatggaaataggtgcataattttatgtgcttggtacatatgagttcaatagata + tgtagtagttggatagtagatagtaaggatatataatcatggaagggtgacgcacaacaa + atcacataatgagttatgattttcactgttctcatctgtttcccctacaaggcgtgatca + ataacaaacaatttcattaaaaaaaaaaaaaccaagtcactctaacatacgtaataactg + tgtatacaaaagaaaaataatatctgttcgtcatctagtttaatacggcaactagttttt + caccagagttcttaccgattttaatgtcttccaggatacgaggaacatcgtaaagcccgt + tcttatagacccttgctggaatatggtgaatttgcccatcactaatctttggattgatga + acttgacgaattcggtggcagctctcctggcttctgggtcagcagggaaagtaatgccac + caaatggtacttcatggccgcctattgaatacagtcttgttctgtcaatagtgacatttt + gcctcctattctcctttttgacgttttcttctgtcaaattagttaattcgacaacggtag + catcctgtttatcggccgcacatttgtacacttgttgaagcgtattttgattcgcgacac + agtcgactaaatacgagatattgttgtacttgtgtttaatttgttctaccacgtcaatat + catggtaatcaaatagttgatcagcaccatattctttcaacagtttttcgtgtttccgag + aagccacaacaatgatcttggtgaagccattcaatttattggctaattggatgagcgact + gacctactgcagttgcaccgccccataataagatggggccgtttctttgtggggttgatg + gctcccacttcaggttcaagcccaagttataggtcaacaccaagccagctgtggtcagtg + acactgggatagtggctgccccttccaaagacctgacagggccggcaggtagaacatctt + cacccaaaaatttgagttcattgggtgatttgtaggcaaccacagttgaaatagcagaat + attcagcaaaagcaccattggaaggaaaccttacggaagatccgtgaatgaacccataaa + tataatcaccaatagaaaagtctttaggatcgacggctgggcccaatttgacaatttggc + cggcagcgtcacatcccagaatagatccttgaggcccgaccttgtagtcaatgtgtgccc + aatcagtcgggttaccagcaacagcgagtgtcttaatcaatacgaatccttcttccaatt + caggaatgggaacgccctctttgacaaccgctttaccgtcttcaatgacgacagccttca + tggtttctggaattgcaacttgcctgtttgtaggttatatgtaattgtcgttgataatct + gtattcagcttgccattttatagaatatggttagaaggatcttaagctagataaagaaaa + agaaaaaaatagaaaaaagcctgcccgctttataagaacttttctcaatatctgttgagg + aacacttagtaatattgtctatcatggttgctttattcttggtgatattactctctggga + ttcgatgtgttctcatcatctgaagggtttcctatgtgttgaaagcctatcccactagac + ggagccgcgcgccagccgggcggatccgagacatcttagtcagctgagacaagaaggcac + agctctcggacaggaactgctgtcatatgaaaacctaaaaatgtttcctggaaattccat + gactgcgcaaagtttgatacgtttattgttcttctctactaccgcactttctatctcgga + aacctttcaactgaatgaactattgttaattgtatggcgtgtggccttgctatttcagca + aatttagagtaagtaaactccgtcggaccagtatagtaacgaaacaagccattgattctg + cagcatattgcgaccaaaacctggaaattggtggctatgagttttaatttataattatat + gaagacaaagcttggaatgctgagtactttttcttcaatgctgctcactgcccagaagcg + ttcagaacaactctactggagccattctgcttgaaggaggataaaaatgccaagatcaag + tcttttaaagactctatcccttttcatcggaaaagcactacaagtttattctacagaagt + ggacaaactgtggaacttgatccatactgagtaagtatggaagactgttaacttggagga + tttttctctgctttctaaagaaggttcactgctttacactcacctaggttctcatgagta + ttgctagctagtgcgagtttgatctatacttcacagacttttaacaccccgttctttaag + ttttccctgattatcaagcggatttccaattcttattccccactatcgcacctttcttat + ctgaattgtatttttgagtggcctaactgtaatcttttattttgttttctaattattgaa + ggcggtttctcgaaacatttcacgatttccaaaattcaagaattgaaattatgaacatag + aagacaaaatgccttttttatcaactattatgaattctctagaaagtggttgcaaaggaa + ataaatatgtatttgctgagaggaaaagtttggaagaacaaaaagtatttctttgatgga + gctgattatcaagcattctttacggggagctttcgaaattttttatattcaaggacattt + atgttgtgtgggttattaaacgtgggactttggaaatacatccaagaagcacaattgagc + tctaacgatgaaccgttaccataaagatagagacatttcttttgacttagaagctacatg + gagtaccaactattaatacacctctcctagagaacatttttaaaatagaataaaataaag + tagcgctctcaactacccttacacttttttccttcatatctggctagaaaagagggatta + catctcggaatcaaagttttaatactgctactacgaactggttacaattctatttttatc + atacacgatactttgaacatcaacttctctcaataccatcgccttcagcggctacaatta + cgtaattgcaccaagcattcgccgagagtttacatatttgcagaccctaacagtatacga + ctcccaacacacgtttaatgttgtacgaattcaagagatggaaatatttcttccgcggaa + gccgtttaaagcagactgaatttaggcttagacaataagcttctgaacgagggtccaaat + ctaaaattcggatattctttttgaccgaaagcggaaacttcacaggcgcgcgtaaaaggg + ttttgcaaggcaccccggcacccctataattggcattcccacattctgcgcatacgaata + cacatgagtgccataaaacaatactccacatacgaaacctatgaaagatgtatgattcgt + tcagaaacaagagcatctccatagagataatgagattgtgtgaaagatgagatatggagg + atatgtgaggtgctaaatgagcgtctgttaacgaatcgtttaagtagtgatgacagatgg + aggaacgggtataaatagagctgcttcaactatatgcctttgaggtatgcgctttcattt + cactatttcttgttaagattacatcagcactaacaaacaaaacaaatacaatggtcaaat + taacttcaatcgctgctggtgttgccgccatcgctgccggtattgccgctgccccagcca + ctaccactctatctccatctgacgaaagggtcaacttggtcgaattgggtgtttacgtct + ccgatatcagagctcatttggctcaatactacttgtttcaagcagctcatccaactgaga + cctacccagttgagattgctgaagctgttttcaactatggtgacttcaccactatgttga + ctggtattccagctgaacaagtcaccagagtcatcactggtgtcccatggtactccacta + gattgagaccagccatctccagtgctctatctaaggacggtatctacactgctattccaa + aatagagacatgcctttactaatgaacttccatagacattgaaaaataacgaaagaataa + aaaaaaaatctttttttttttttgatataaatacagttgactatataaacatatatcaat + actataagcctatatttgatcaagacgacattatttaggtgcatcaacaacggaaaagaa + actttcttctaggctatccttctggggaagcagttgaaagtgctatccagtattattgca + tatattcttaatgagtagtgctgaagtttgatacgtttggctctgttgcttttatttctc + ccacgaaaataaattgatttctttatttttcagtgtgttggcccggagttgtttacacac + atgtctctttttggattaatgctgtagatcagggactatgcgagcgacaagtcagagtaa + ccatattgaatttttcgtctcctgttatacgaatatttgcatttctgtgcctacttatgt + gtgtatatgtttttattagtcagaaaatggagtaagtgttaaagtaaggatagtgttaag + gaggtaataactaaagatttgtaggataaggcggtgttatttgttgcaattttctcaggg + gtggagtggaaatggtttgattcaattatcagttaagcaaggaagagttgaagggcgaac + atttgcaataatcccaatgtcatttaatgcgggtcggctgagacgctcaatattcggccg + aaaagtatcattcggccgcataataaaatggaatagaagataaagcagatgtcactaatt + gagcaccaaaactctgagtagaaattatataaagaagcacgaacatgacctgctttagat + gttgcttgatattattgaaataaaaaaaaacatagaaccactgaaaaatacaaaaaaaaa + atgctttacccagaaaaatttcagggcatcggtatttccaacgcaaaggattggaagcat + cctaaattagtgagttttgacccaaaaccctttggcgatcatgacgttgatgttgaaatt + gaagcctgtggtatctgcggatctgattttcatatagccgttggtaattggggtccagtc + ccagaaaatcaaatccttggacatgaaataattggccgcgtggtgaaggttggatccaag + tgccacactggggtaaaaatcggtgaccgtgttggtgttggtgcccaagccttggcgtgt + tttgagtgtgaacgttgcaaaagtgacaacgagcaatactgtaccaatgaccacgttttg + actatgtggactccttacaaggacggctacatttcacaaggaggctttgcctcccacgtg + aggcttcatgaacactttgctattcaaataccagaaaatattccaagtccgctagccgct + ccattattgtgtggtggtattacagttttctctccactactaagaaatggctgtggtcca + ggtaagagggtaggtattgttggcatcggtggtattgggcatatggggattctgttggct + aaagctatgggagccgaggtttatgcgttttcgcgaggccactccaagcgggaggattct + atgaaactcggtgctgatcactatattgctatgttggaggataaaggctggacagaacaa + tactctaacgctttggaccttcttgtcgtttgctcatcatctttgtcgaaagttaatttt + gacagtatcgttaagattatgaagattggaggctccatcgtttcaattgctgctcctgaa + gttaatgaaaagcttgttttaaaaccgttgggcctaatgggagtatcaatctcaagcagt + gctatcggatctaggaaggaaatcgaacaactattgaaattagtttccgaaaagaatgtc + aaaatatgggtggaaaaacttccgatcagcgaagaaggcgtcagccatgcctttacaagg + atggaaagcggagacgtcaaatacagatttactttggtcgattatgataagaaattccat + aaatagtctatatacgtaatatttttcagaatctcttaaaatgcatgtcaaatctcggaa + tttatcatgacccattacataacaagagaaaagtggtttgcattgtatgtataccttaaa + ccaattcttcggtagtcacatgacctatatatagggacattcatttcttgaataggatat + ttcttaaacttaagggaaattttgatgaaaatttagaatctctcgacaacagtgcagagc + agtaagttttattcaatacgaaatgtgtgcagggttttacccattcatgtcgagtacgtc + tacatttgttctgcgatttaacttaatacttagcctactcatatgtatcgaagccaaact + attaaatctgaaaactgaatacccattaagtaggtacattaatgcaattttcgtccgaaa + ggtagagtgttaatcatgcttttgcttcgaaatctagagtccgagcatgtctgatgaggt + cctcttaaagcacttcaattttaaaagagaagagcgcaaatttttacaaagaatgcctat + attaatatactgtgctatctaagaggatggttcaatggtttgaaatgagttgaaaaataa + agatttacatagtagcgtgtattcttcacatttttgcattcggtagattttctactcaag + acccgtgcagatcactattacactcattattacaagtcattgagaacgaaattttgacat + cattactaataatgttactcattaatactttattaatgtaagcggtagataaacaaatga + acatgaaattggctgctgaaatgctctgatggattcgattacagtaaaaaaacctcggtt + aagattggtttgcctgcaatgcaaaaagatcaaacggaaatgtgataaactgcggcctgc + ttgctcgcgatgccaacaaaattcattacagtgtgaatatgaagagagaacagatttatc + tgccaatgttgcagcaaacgactctgatggattcaattcctctcataagctcaatttcga + acagcaacctgtacttgaaaggactgggcttagatattccttacaagtgcctgaaggtgt + cgttaatgctacgctgtcgatatggaacgccgaagatatgctagttatagtaggattagt + tacatttctggattatccttttgctgcgcatagtctggcgcaacatgaccagtatatcag + ggcactttgtgcttcgttgtacggcatggcgcttgttgactttagcaattatgctaatgg + tattccttgtgaagacacatcaagaagtatactaggaccattgtcattcatagaaaaggc + catttttagacggatagaacatagtaagcaatttcgagttcagtctgccgccttagggtt + attatacaatgcattttcaatggaagaagaaaacttctcgactcttctaccgtcactcat + cgctgaagtggaagacgtgttgatgcaaaaaaaagactgtgaaatacttttgaggtgttt + ctatcaaaatatttatcccttctatccttttatggacatttcactctttgagagcgatct + cactagtttgcttttacaagacgacaataatcgttggaaaattagtactgaagttaaaaa + tgtgcgcaaaaaaatagaaactttgtcattacttacaatagtaatggccatggccttgat + gcattcaaaattggatgcaaatcttctttcaatggtaaaagaaaatgcctccgaaagtgc + caggaaactttctcttttatgtcataaactattatgcctcctggatgtatttcgctatcc + aaatgagaacacttttacttgccttttatatttctacgtttcagagcatttagatcccga + gagtcccgattgtgtactgagccccactaacttgcttactctgcaccatcttttaaattt + gtccatgaccttaggtcttcaatatgagccttcgaagtacaaacgtttcaaagatccaga + agtgataaggcagagacggatattatggttaggagttcagtcattactttttcaaatttc + tcttgctgaaggtgatgctggtaaatcaaatagtgaatatatggaggcatatttaacaga + cttcgaagaatatattgaagcttcctcagagtatgaaaaaagttctgcgagtgaatcgaa + cgtgcaaatgaatgatattgtttggaataagtacaaatttcacgtcattttgagtaaact + aatgtctgattgcacttcagttatacaacatccgcagcttttccacattttaggaaatat + taaaagatctgaagattttatggctgagaactttcctacaagttcgatttaccaacccct + tcatgaaaaggaaccaaatgcgatcaaagttggcaaaagtacggttctcgatgtcatgga + tattcaaaaaactgaaatatttcttacaaatattgtgggaagtatgtgttttttaaacat + ttttgatgtcctatcgttacattttgaaaaaaaatgtgttatgcactgggaagaatatga + aaagaactatcatttccttactttgaaaagtttcaatgcatacttaaagctagcagggtt + gatatctgattatctcgagaataagtttcaagggaacattttagagagtcgcggttatat + catagataaacaaatatgttttatgcttgtaaggatctggatgttccaatgtcgtatttt + gttaaggttttcatacaagcaagaaagtcagaaaaaattggcctcttccagtatatccac + taacgataatgaaaaagaagatgaaatgattgtcattttagaaagacttattaaacacat + tcgtaaccaaatggcacatttagtggatctagcaaagggaaaacttcaagatagttactt + tggtgcttaccaaactgttcccatgtttagatacgttgtgtatttgatcgatgttggcgg + cttagtatctgtgacaaatgggttttgggataagatttccagtgatggtgaaataccgcc + aaaagtacaacaagccgtgagattgaaatggggattggactgcaataattcgagaagaat + caaacaaaagttaataagcagccagagtttgcagagtttcaatcaagttctgttgtgcca + gatggaggatgcagttctctccagttccttcgcaataaaagccaataccgctatgtccca + aaacacggctgaagaatttttcaatatcagcgaagaagaggctttaaatcaactattgga + aaacaacaattttgatgccttctgggatttattaggtgaaaatctgagcgatatgccttc + tttgtgagaatacggatatttggaaaggctaaaaaaaaaaaaacaagccaaaaacaagaa + acactgttatcctccacgttttttccactgttttaagactcgacaagacggaaagattcc + tcacatttcgcagtgtttcttttaacctgctcgtcaatttgaaaaaatgagcatggttag + gcaggcggaatgtaataggtgcggtgcgtataaataagtatctcggccgttcctctattg + cgctcggccgagatgtcctacatgcataaaatagattaaaaaaagggatcacgatactca + aatatgcatataataacaacaacacactttgcggtaaatagaaggataatatcgtaatct + tttgctggtttcgatgatgacttgaaaaaattcaattactctttcgatcatctagaagaa + gcatatccatcgtgcttaaaatgattgggtccgcgtccgactcatctagcaagttaggac + gcctccgatttctttctgaaactgccgctattaaagtatccccgttaatcctaggagaag + tctcatacgatggagctcgttcggattttctcaaatcaatgaacaagaatcgagcttttg + aattgcttgatactttttacgaggcaggtggaaatttcattgatgccgcaaacaactgcc + aaaacgagcaatcagaagaatggattggtgaatggatacagtccagaaggttacgtgatc + aaattgtcattgcaaccaagtttataaaaagcgataaaaagtataaagcaggtgaaagta + acactgccaactactgtggtaatcacaagcgtagtttacatgtgagtgtgagggattctc + tccgcaaattgcaaactgattggattgatatactttacgttcactggtgggattatatga + gttcaatcgaagaatttatggatagtttgcatattctggtccagcagggcaaggtcctct + atttgggtgtatctgatacacctgcttgggttgtttctgcggcaaactactacgctacat + cttatggtaaaactccctttagtatctaccaaggtaaatggaacgtgttgaacagagatt + ttgagcgtgatattattccaatggctaggcatttcggtatggccctcgccccatgggatg + tcatgggaggtggaagatttcagagtaaaaaagcaatggaggaacggaggaagaatggag + agggtattcgttctttcgttggcgcctccgaacaaacagatgcagaaatcaagattagtg + aagcattggccaagattgctgaggaacatggcactgagtctgttactgctattgctattg + cctatgttcgctctaaggcgaaaaatttttttccgtcggttgaaggaggaaaaattgagg + atctcaaagagaacattaaggctctcagtatcgatctaacgccagacaatataaaatact + tagaaagtatagttccttttgacatcggatttcctaataattttatcgtgttaaattcct + tgactcaaaaatatggtacgaataatgtttagataatttttcagtaatcaactacgcaag + taaagcagtaaatacgttactgctggtattaatgtcatgtattgaggcaatgatgctatg + ctcttaacgacatgtagcttacaaaacgttcctatggttttactgcatggtatttacaaa + ttagatagcagttccatccgcccttgacatattattattcagacaaggtgtatatgagca + taaatatgtatatatgcacatgaggtttgtattaaccttgcaactgtaccaaaatacaat + cttctttgctgctatttaacggcgtatgtgcagttcataaatgcgtgttctgtatagtac + atccgcgcacattcttcctagcggaagacatattaacgtagcccgtacgccctggttaag + acttggtatagttccaataattggaatattactgcatagtggtccaatagcaggatttag + cataaacatgataatatttagagatgctttcatccctctgacgaaggtggtagagaacaa + gaaaatgaataacattatatcatattcctatatatatatatatatatatatatatatcat + ataacggtggaaaatcccggcgatattacagataaacattacacccgcatgaatgtgagc + cactactatattataacttagtgaataaagagtgttacaatagtgaggtgcatattattc + taatgtaaggtctgtataagtacgaaatattctgaagtggcatccagtcaagcaaatggt + aatattaaggaacttttaagttaatgacgtcatggtagtgctcgtacttcaagtcaaagt + gtttatgtattatggttgaagaatagaatatttttatgtttaggtgattttagtggtgat + ttttctgtaatattgacataagtgtatataaattaagtggttagtatacggtgaaaaaga + ggtataacgtatgtattaagggaatttatacgatatttgggcccgccgaatgagatatag + atattaaaatgtggataatcgtgggctttatgggtaaatggcacagggtatagaccgctg + aggcaagtgccgtgcataatgatgtgggtgcatttggtactgatttagtgagaatgggcc + atggattggagtgtgagagtagggtaacttgagagtggtatatactgtagcatccgtgtg + cgtatgccccatcaatataagtgaaggtgagtatggcatgtggtggtggtatagagtggt + agggtaagtatgtatgtattatttacgatccggggaattcttacctataatagcggctcg + ctgtccgaacgattgcgccgcacttttctgcctcttacattcccttttcctaaggcgata + agcaggctgaatggaaactacgatataacgcagtagaggtcccccaagagctggaaagaa + aatattggtttgatagccagcactattctatccagtgggctcttgtaccattttctgtgg + tatggctttgattcaactgcatacgtagttaacgcgatcagccgcacatgggcattatca + ttttcaggggtactggtaaaccctattgtttagaaagaatacctggtaagcgggtctgtc + gagtctttcccaactaattttgaagaaaaaatctttacctagtttgagaataatcgt diff --git a/src/squirrel-1.4/tables/README b/src/squirrel-1.4/tables/README new file mode 100644 index 0000000..ff69b38 --- /dev/null +++ b/src/squirrel-1.4/tables/README @@ -0,0 +1,5 @@ + Dir: $SQUIRREL/tables/ +Date: Fri May 07 12:42:56 BST 1993 + +This directory contains squirrel databases. Please read +SysManagement. \ No newline at end of file diff --git a/src/squirrel-1.4/tables/SysManagement b/src/squirrel-1.4/tables/SysManagement new file mode 100644 index 0000000..70b7aec --- /dev/null +++ b/src/squirrel-1.4/tables/SysManagement @@ -0,0 +1,259 @@ + SQUIRREL v1.3 + System Management + + Simon Dear + 29 June 1992 + + +0. Introduction +--------------- + +Squirrel is a complicated script that gathers together information +about an experiment and then assembles the processed sequences into a +user's database. The philosophy behind squirrel is that there should +be minimal user interaction. In actuality, all that need be entered is +little more than the name of directory containing data to be +processed. + +Ease of use for the user often means extra work for someone else. With +squirrel this is no exception. Squirrel requires the maintenance of +several small files. This document describes these files and the +procedure for keeping them updated. + +Squirrel needs to gather additional information about: + + a) subclone libraries + b) vectors used to clone each cosmid + c) sequencing vector clone sites + d) files containing vector sequence + e) user's databases + +For a sequencing project the data on (b)-(d) will be fairly static and +quite small. Data on (a) will be frequently updated as new libraries +are going to be continually created. Data on (e) will be updated +occasionally; every time new databases are started. + +For C-Shell programming simplicity and uniformity the data is +encapsulated in simple awk programs. + +We sometimes need to look up information which is a generalisation +about a specific example. For example, we may be want data on a +subclone e06b11 where data is held for all subclones starting e06. The +pattern matching abilities of awk are ideal for this. + +We also sometime need to determine information about a specific +example, where it is not possible to make general inferences using +simple rules. An example is data on (b), which reflects the +changing strategies during genome mapping. Building this data into an +awk program is a very simple task. See Appendix 1. + +However, is it not desirable to maintain awk programs. Small typing +mistakes can result in a program with severe errors. For (a), and +(c)-(e) it is more convenient to enter the data into a text file, and +have the awk program automatically created from it using the UNIX make +facility. In the case of (b), there is no escaping having an awk +program. It is therefore fortunate that this data will not +substantially change during the lifetime of the sequencing project. + +The text files for (a)-(e) can be found in the directory + + ~wormpub/BioSW/squirrel/tables + +During the rest of this document it is assumed that the person +performing the updates is logged in as user wormpub and that they have +changed to this directory. + + + + +1. Updating information on subclone libraries +--------------------------------------------- + +The information on subclone libraries can be updated in two ways. + +First, the file ``subclones'' can be modified using an editor. It is +important that information is entered in exactly the right format. The +fields of this file are: + + ... + + +An example: + e50 F54C8 1000..2000 shear M13mp18 + +Alternatively the program update_subclones can be used. It provides +sensible default values for all the fields and checks that the +data specified is valid. The program can be run by any user from any +directory. It assumes that the environment variable SUBCLONES holds +the full path name of the file ``subclones''. + +Here is an example of an update of the subclone file using +update_subclones. Default values are given in square brackets. +Responding with a question mark ('?') at any time provides information +of what is expected. + + + Maintain subclone database + + Database=/nfs/al/hgmp0/wormpub/BioSW/staden/tables/subclones + + Microtitre dish [e50] ? + Cosmid [F54C8] ? + Method of fragmentation [shear] ? + Vector [M13mp18] ? + Size range [1000..2000] ? + Comment #1 [] ? + + Here are the values you entered: + + Microtitre dish number: e50 + Cosmid: F54C8 + Fragmentation method: shear + Vector: M13mp18 + Size range: 1000..2000 + + Are these values correct [Yes] ? + + +Once the file has been updated it is important to remake the +awk program required by squirrel. To do this, type: + + make all + + + + +2. Updating information on vectors used to clone each cosmid +------------------------------------------------------------ + +Information on which vectors are used for each cosmid can be found in +the file ``clone_types.awk''. This information is be updated using an +editor, though it is unlikely that it will change much through the +course of the sequencing project. The data is only encapsulated in an +awk program; there is no text file. It is important to update this file +correctly and to ensure that no errors are introduced into the awk +program. The program can be tested for errors by typing something +like: + + echo TEST | awk -f clone_types.awk + + + + +3. Updating information on sequencing vector clone sites +-------------------------------------------------------- + +Information on sequencing vector clone sites is held in the file +``seqclone-sites'', and is updated by manually editing the text file. +The format of the data is: + + + +An example: + pBS 2305 46 -79 + +The positions relate to the sequence held in the file specified in the +``vector-seqfiles'' files. The clone-site is defined as the base +position of the 3' end of the cloning site The primer sites are positions +relative to this. + +To make the awk program required by squirrel, type: + make all + +It is important that this information is correct and that the sequence in +the sequence file is in the correct orientation. Otherwise, the vector +will not be successfully removed from sequence processed by squirrel. + + + + + +4. Updating information on files containing vector sequence +----------------------------------------------------------- + +Information of the files containing vector sequence is help in the file +``vector-seqfiles'', and is updated by manually editing the text file. +The format is: + + + +An example: + pBS /nfs/al/pubseq/pubseq/inhouse/sun-nematode/squirrel/seqs/blue.vep + +To make the awk program required by squirrel, type: + make all + +Note that the file name includes the full path name. All working versions +of sequence files required by squirrel are kept in the $SQUIRREL/seqs +directory. + + + + +5. Updating information on user's databases +------------------------------------------- + +User's databases must be registered with squirrel by including an +entry for the project in the file ``databases''. The format is: + + + +An example: + C38C10 C38C10 C38C10 0 bap kt + +The lookup file is remade by typing: + make all + +The directory is relative to the owner's home directory. The database type +is currently one of "dap" or "bap". So the database for kt's cosmid C38C10 +is ~kt/C38C10/C38C10.??0. + + +A1. Vectors used to clone each cosmid +------------------------------------- + +#clone_types.awk +# +# Determine Clone types from Clone names +# +# 3 February 1992, Simon Dear +# +# Assumes that there is one clone name per line. +# Outputs to stdout clone vector. +# +# Data in this program has been taken from "Clone types in the genome map +# of Caenorhabditis elegans" courtesy by John Sulston. This needs +# revision. +# +# Possible usage: +# set clone-type = `echo $clone | awk -f clone-types.awk` +# +/^A/ { print "Lambda"; next; } +/^ZL/ { print "Lambda"; next; } +/^YSL/ { print "Lambda"; next; } +/^[BCDERM]/ { print "pJB8"; next; } +/^ZC/ { print "pJB8"; next; } +/^K/ { print "LoristB"; next; } +/^T/ { print "Lorist2"; next; } +/^W/ { print "Lorist4"; next; } +/^F/ { print "Lorist6"; next; } +/^ZK/ { + num = substr($1,3) + 0.0; + if (num < 15) { print "unknown"; next; } + if (num <= 56) { print "Lorist6"; next; } + if (num <= 130) { print "Lorist2"; next; } + if (num <= 164) { print "Lorist6"; next; } + if (num <= 177) { print "Lorist2"; next; } + if (num <= 192) { print "Lorist6"; next; } + if (num <= 344) { print "Lorist2"; next; } + if (num <= 354) { print "Lorist6"; next; } + if (num <= 514) { print "Lorist2"; next; } + if (num <= 552) { print "Lorist6"; next; } + if (num <= 596) { print "Lorist2"; next; } + if (num <= 616) { print "Lorist?"; next; } + if (num <= 626) { print "Lorist2"; next; } + if (num <= 692) { print "Lorist6"; next; } + if (num <= 756) { print "Lorist2"; next; } + if (num >= 757) { print "Lorist6"; next; } + } +/^Y/ { print "pYAC4"; next; } diff --git a/src/squirrel-1.4/tables/all_pages b/src/squirrel-1.4/tables/all_pages new file mode 100644 index 0000000..744133c --- /dev/null +++ b/src/squirrel-1.4/tables/all_pages @@ -0,0 +1,22 @@ +#! /bin/csh -f +set FORMAT = ~wormpub/BioSW/staden/tables/format.awk +set SUBCLONEDB = subclones +if (-e subclones) then + set SUBCLONEDB = subclones +else + if ($?SUBCLONES) then + set SUBCLONEDB = $SUBCLONES + if (! -e $SUBCLONEDB) then + echo "Cannot open file $SUBCLONEDB" + exit 1 + endif + else + echo "Cannot open file" + exit 1 + endif +endif + +echo "Printing $SUBCLONEDB on lp" + +awk -f $FORMAT $SUBCLONEDB | tbl | ptroff -Plp +exit 0 diff --git a/src/squirrel-1.4/tables/clone-types.lookup b/src/squirrel-1.4/tables/clone-types.lookup new file mode 100644 index 0000000..1b2770f --- /dev/null +++ b/src/squirrel-1.4/tables/clone-types.lookup @@ -0,0 +1,46 @@ +# +# Database to determine clone types from clone names +# +# Example: +# +# This is an example from the C.elegans sequencing project. +# +# Assumes that there is one clone name per line. +# Outputs to stdout clone vector. +# +# Data in this program has been taken from "Clone types in the genome map +# of Caenorhabditis elegans" courtesy by John Sulston. This needs +# revision. +# +# Possible usage: +# set clone-type = `echo $clone | awk -f clone-types.awk` +# +/^A/ { print $1,"Lambda"; next; } +/^ZL/ { print $1,"Lambda"; next; } +/^YSL/ { print $1,"Lambda"; next; } +/^[BCDERM]/ { print $1,"pJB8"; next; } +/^ZC/ { print $1,"pJB8"; next; } +/^K/ { print $1,"LoristB"; next; } +/^T/ { print $1,"Lorist2"; next; } +/^W/ { print $1,"Lorist4"; next; } +/^F/ { print $1,"Lorist6"; next; } +/^ZK/ { + num = substr($1,3) + 0.0; + if (num < 15) { print $1,"unknown"; next; } + if (num <= 56) { print $1,"Lorist6"; next; } + if (num <= 130) { print $1,"Lorist2"; next; } + if (num <= 164) { print $1,"Lorist6"; next; } + if (num <= 177) { print $1,"Lorist2"; next; } + if (num <= 192) { print $1,"Lorist6"; next; } + if (num <= 344) { print $1,"Lorist2"; next; } + if (num <= 354) { print $1,"Lorist6"; next; } + if (num <= 514) { print $1,"Lorist2"; next; } + if (num <= 552) { print $1,"Lorist6"; next; } + if (num <= 596) { print $1,"Lorist2"; next; } + if (num <= 616) { print $1,"Lorist?"; next; } + if (num <= 626) { print $1,"Lorist2"; next; } + if (num <= 692) { print $1,"Lorist6"; next; } + if (num <= 756) { print $1,"Lorist2"; next; } + if (num >= 757) { print $1,"Lorist6"; next; } + } +/^Y/ { print $1,"pYAC4"; next; } diff --git a/src/squirrel-1.4/tables/create.lookup b/src/squirrel-1.4/tables/create.lookup new file mode 100644 index 0000000..c584625 --- /dev/null +++ b/src/squirrel-1.4/tables/create.lookup @@ -0,0 +1,3 @@ +#! /bin/csh -f +# +sed 's/^\([^ ]*\)/\/^&\/ {print "&/;s/$/";}/' $1 >! $2 diff --git a/src/squirrel-1.4/tables/cron-job b/src/squirrel-1.4/tables/cron-job new file mode 100644 index 0000000..021c32f --- /dev/null +++ b/src/squirrel-1.4/tables/cron-job @@ -0,0 +1,6 @@ +#! /bin/sh + +if test -d /nfs/al/pubseq/pubseq/inhouse/sun-nematode/squirrel/tables ; then + cd /nfs/al/pubseq/pubseq/inhouse/sun-nematode/squirrel/tables + make all +fi diff --git a/src/squirrel-1.4/tables/databases b/src/squirrel-1.4/tables/databases new file mode 100644 index 0000000..dac0099 --- /dev/null +++ b/src/squirrel-1.4/tables/databases @@ -0,0 +1,18 @@ +# +# A database of Staden databases currently using squirrel: +# +# Format: +# +# +# +# Example: +# +# We are sequencing clone CLONE. We have created a bap database +# called T in the subdirectory TEST of user sd's home directory. +# (Database files would be called ~sd/TEST/T.{SQ,AR,RL,TG,CC}0) +# CLONE would be the name of the project in the edited version of +# the script 'squirrel' that has been copied to ~sd/TEST/. +# +# CLONE TEST T 0 bap sd +# +TESTDB TESTDB TESTDB 0 bap sd diff --git a/src/squirrel-1.4/tables/databases.lookup b/src/squirrel-1.4/tables/databases.lookup new file mode 100644 index 0000000..5b93807 --- /dev/null +++ b/src/squirrel-1.4/tables/databases.lookup @@ -0,0 +1,18 @@ +/^#/ {print "#";} +/^#/ {print "# A database of Staden databases currently using squirrel:";} +/^#/ {print "#";} +/^#/ {print "# Format:";} +/^#/ {print "#";} +/^#/ {print "# ";} +/^#/ {print "#";} +/^#/ {print "# Example:";} +/^#/ {print "#";} +/^#/ {print "# We are sequencing clone CLONE. We have created a bap database ";} +/^#/ {print "# called T in the subdirectory TEST of user sd's home directory.";} +/^#/ {print "# (Database files would be called ~sd/TEST/T.{SQ,AR,RL,TG,CC}0)";} +/^#/ {print "# CLONE would be the name of the project in the edited version of";} +/^#/ {print "# the script 'squirrel' that has been copied to ~sd/TEST/.";} +/^#/ {print "#";} +/^#/ {print "# CLONE TEST T 0 bap sd";} +/^#/ {print "#";} +/^TESTDB/ {print "TESTDB TESTDB TESTDB 0 bap sd";} diff --git a/src/squirrel-1.4/tables/format.awk b/src/squirrel-1.4/tables/format.awk new file mode 100644 index 0000000..7527695 --- /dev/null +++ b/src/squirrel-1.4/tables/format.awk @@ -0,0 +1,58 @@ +BEGIN { + entries_per_page = 50; + PP = 0; +} +{ + if ( (NR % entries_per_page) == 1 ) { + if (PP) print ".bp"; + PP++; + print "Page - " PP; + # + print ".sp 3"; + print ".TS"; + print "box, tab (%);"; + print "c s s s s"; + print " c | c | c | c | c | c"; + print " c | c | c | c | c | c"; + print " l | l | l | l | l | l."; + print "Cosmid Subclone Libraries"; + print "="; + print "Microtitre%Cosmid%Size Range%Fragmentation%Vector%Comments" ; + print "Dish Number%%(bases)%Method%"; + print "="; + } + + count = 0; + if (NF > 5) { + line = $0; + s = index(line,"("); + t = index(line,")"); + while (s!=0 && t!=0) { + comment = substr(line,s+1,t-s-1); + line = substr(line,t+1); + if (count==0) { + print $1 "%" $2 "%" $3 "%" $4 "%" $5 "%" comment; + } else { + print "%%%%%" comment; + } + count++; + s = index(line,"("); + t = index(line,")"); + } + if (count>1) print "%%%%%_"; + } + if (count == 0) + print $1 "%" $2 "%" $3 "%" $4 "%" $5 "%"; + + if ( (NR % entries_per_page) == 0 ) { + print ".TE"; + } +} + +END { + if ( (NR % entries_per_page) != 0 ) { + # fill up page + for(i=NR; i % entries_per_page; i++) print "%%%%%"; + print ".TE"; + } +} diff --git a/src/squirrel-1.4/tables/last_page b/src/squirrel-1.4/tables/last_page new file mode 100644 index 0000000..7b6b71f --- /dev/null +++ b/src/squirrel-1.4/tables/last_page @@ -0,0 +1,27 @@ +#! /bin/csh -f +set FORMAT = ~wormpub/BioSW/staden/tables/format.awk +set SUBCLONEDB = subclones +if (-e subclones) then + set SUBCLONEDB = subclones +else + if ($?SUBCLONES) then + set SUBCLONEDB = $SUBCLONES + if (! -e $SUBCLONEDB) then + echo "Cannot open file $SUBCLONEDB" + exit 1 + endif + else + echo "Cannot open file" + exit 1 + endif +endif + +echo "Printing last page of $SUBCLONEDB on lp" + +set a = `wc -l $SUBCLONEDB` +# entries_per_page is also explicitly defined in $FORMAT +set entries_per_page = 50 +@ b = ( ( ( $a[1] - 1 ) / $entries_per_page ) * $entries_per_page ) + 1 +tail +$b $SUBCLONEDB | awk -f $FORMAT | grep -v "^Page -" | tbl | ptroff -Plp + +exit 0 diff --git a/src/squirrel-1.4/tables/lookup.csh b/src/squirrel-1.4/tables/lookup.csh new file mode 100644 index 0000000..277de3e --- /dev/null +++ b/src/squirrel-1.4/tables/lookup.csh @@ -0,0 +1,6 @@ +#! /bin/csh -f +# +# Usage: +# lookup key file +# +echo $1 | awk -f $2 diff --git a/src/squirrel-1.4/tables/makefile b/src/squirrel-1.4/tables/makefile new file mode 100644 index 0000000..dcbff53 --- /dev/null +++ b/src/squirrel-1.4/tables/makefile @@ -0,0 +1,22 @@ +# +# Make look up tables for files +# +TABLES= \ + databases.lookup \ + seqclone-sites.lookup \ + subclones.lookup \ + vector-seqfiles.lookup \ + screens.lookup + + +%.lookup : % + @echo "" + @echo Building $<.lookup + @rm -f $<.lookup + @./create.lookup $< $<.lookup + +all: $(TABLES) + +clean: + rm -f $(TABLES) + diff --git a/src/squirrel-1.4/tables/screens b/src/squirrel-1.4/tables/screens new file mode 100644 index 0000000..4a58f8d --- /dev/null +++ b/src/squirrel-1.4/tables/screens @@ -0,0 +1,13 @@ +# +# Database of sequences to screen against clones +# +# Format: +# +# +# +# Example: +# +# We wish to screen our cosmid CLONE against yeast chromosome 3. +# +# CLONE scchrIII.seq +# diff --git a/src/squirrel-1.4/tables/screens.lookup b/src/squirrel-1.4/tables/screens.lookup new file mode 100644 index 0000000..3f35816 --- /dev/null +++ b/src/squirrel-1.4/tables/screens.lookup @@ -0,0 +1,13 @@ +/^#/ {print "#";} +/^#/ {print "# Database of sequences to screen against clones";} +/^#/ {print "#";} +/^#/ {print "# Format:";} +/^#/ {print "#";} +/^#/ {print "# ";} +/^#/ {print "#";} +/^#/ {print "# Example:";} +/^#/ {print "#";} +/^#/ {print "# We wish to screen our cosmid CLONE against yeast chromosome 3.";} +/^#/ {print "#";} +/^#/ {print "# CLONE scchrIII.seq";} +/^#/ {print "#";} diff --git a/src/squirrel-1.4/tables/seqclone-sites b/src/squirrel-1.4/tables/seqclone-sites new file mode 100644 index 0000000..8b15bc7 --- /dev/null +++ b/src/squirrel-1.4/tables/seqclone-sites @@ -0,0 +1,20 @@ +# +# Database of sequencing clone cloning and priming sites +# +# +# +# NOTES: +# clone-site is defined as the base position of the 3' end +# of the cloning site +# +# Example: +# +# Our sequencing vector is M13mp18, our cloning site is SmaI and +# we are using the -20 forward sequencing primer and the -21 +# reverse sequencing primer. With respect to the seqeunce file +# $SQUIRREL/seqs/M13mp18.seq we would have: +# +# M13mp18 6249 41 -24 +# +M13mp18 6249 41 -24 +pBS 2305 46 -79 diff --git a/src/squirrel-1.4/tables/seqclone-sites.lookup b/src/squirrel-1.4/tables/seqclone-sites.lookup new file mode 100644 index 0000000..92761bf --- /dev/null +++ b/src/squirrel-1.4/tables/seqclone-sites.lookup @@ -0,0 +1,20 @@ +/^#/ {print "#";} +/^#/ {print "# Database of sequencing clone cloning and priming sites";} +/^#/ {print "#";} +/^#/ {print "# ";} +/^#/ {print "#";} +/^#/ {print "# NOTES:";} +/^#/ {print "# clone-site is defined as the base position of the 3' end";} +/^#/ {print "# of the cloning site";} +/^#/ {print "#";} +/^#/ {print "# Example:";} +/^#/ {print "#";} +/^#/ {print "# Our sequencing vector is M13mp18, our cloning site is SmaI and";} +/^#/ {print "# we are using the -20 forward sequencing primer and the -21";} +/^#/ {print "# reverse sequencing primer. With respect to the seqeunce file";} +/^#/ {print "# $SQUIRREL/seqs/M13mp18.seq we would have:";} +/^#/ {print "#";} +/^#/ {print "# M13mp18 6249 41 -24";} +/^#/ {print "#";} +/^M13mp18/ {print "M13mp18 6249 41 -24";} +/^pBS/ {print "pBS 2305 46 -79";} diff --git a/src/squirrel-1.4/tables/subclones b/src/squirrel-1.4/tables/subclones new file mode 100644 index 0000000..eb07145 --- /dev/null +++ b/src/squirrel-1.4/tables/subclones @@ -0,0 +1,15 @@ +# +# Database of subclone libraries: +# +# Format: +# +# +# +# Example: +# +# Microtitre dish a01 contains a subclone library for clone CLONE. +# It was created by shearing then cloning into M13mp18. Fragment +# size if selected on range 1000 to 2000 bases. +# +# a01 CLONE 1000..2000 shear M13mp18 +# diff --git a/src/squirrel-1.4/tables/subclones.lookup b/src/squirrel-1.4/tables/subclones.lookup new file mode 100644 index 0000000..2195528 --- /dev/null +++ b/src/squirrel-1.4/tables/subclones.lookup @@ -0,0 +1,15 @@ +/^#/ {print "#";} +/^#/ {print "# Database of subclone libraries:";} +/^#/ {print "#";} +/^#/ {print "# Format:";} +/^#/ {print "#";} +/^#/ {print "# ";} +/^#/ {print "#";} +/^#/ {print "# Example:";} +/^#/ {print "#";} +/^#/ {print "# Microtitre dish a01 contains a subclone library for clone CLONE.";} +/^#/ {print "# It was created by shearing then cloning into M13mp18. Fragment";} +/^#/ {print "# size if selected on range 1000 to 2000 bases.";} +/^#/ {print "#";} +/^#/ {print "# a01 CLONE 1000..2000 shear M13mp18";} +/^#/ {print "#";} diff --git a/src/squirrel-1.4/tables/vector-seqfiles b/src/squirrel-1.4/tables/vector-seqfiles new file mode 100644 index 0000000..a643a83 --- /dev/null +++ b/src/squirrel-1.4/tables/vector-seqfiles @@ -0,0 +1,14 @@ +# database for mapping of vector to sequence file +# +# Format: +# +# +Lambda lambda.seq +Lorist2 lorist2.vep +Lorist6 lorist6.vep +LoristB loristB.seq +M13mp18 m13mp18.vec +M13mp7 m13mp7.seq +pBS blue.vep +pJB8 pjb8.vep +pUC18 puc18.vec diff --git a/src/squirrel-1.4/tables/vector-seqfiles.lookup b/src/squirrel-1.4/tables/vector-seqfiles.lookup new file mode 100644 index 0000000..c30cfc1 --- /dev/null +++ b/src/squirrel-1.4/tables/vector-seqfiles.lookup @@ -0,0 +1,14 @@ +/^#/ {print "# database for mapping of vector to sequence file";} +/^#/ {print "#";} +/^#/ {print "# Format:";} +/^#/ {print "# ";} +/^#/ {print "#";} +/^Lambda/ {print "Lambda lambda.seq";} +/^Lorist2/ {print "Lorist2 lorist2.vep";} +/^Lorist6/ {print "Lorist6 lorist6.vep";} +/^LoristB/ {print "LoristB loristB.seq";} +/^M13mp18/ {print "M13mp18 m13mp18.vec";} +/^M13mp7/ {print "M13mp7 m13mp7.seq";} +/^pBS/ {print "pBS blue.vep";} +/^pJB8/ {print "pJB8 pjb8.vep";} +/^pUC18/ {print "pUC18 puc18.vec";} diff --git a/src/staden/FtoC.c b/src/staden/FtoC.c new file mode 100644 index 0000000..83d0312 --- /dev/null +++ b/src/staden/FtoC.c @@ -0,0 +1,76 @@ +#include "fort.h" + +void Cstr2Fstr(char *Cstr, + char *Fstr, int_fl Flen) +/* + Copy a '\0' terminated C string to a Fortran string, blank padding + if needed and ignoring excess C characters if needed. + + This function works if the strings are distinct or coincident, but + not if they overlap in any other way. +*/ +{ int_fl i; + + for (i=0; (i 0) && (Fstr[FsigLen-1] == ' ')) + { FsigLen--; + } + + /* Copy up to (Clen-1) significant characters */ + i=0; + while ((i < FsigLen) && (i < (Clen-1))) + { Cstr[i] = Fstr[i]; + i++; + } + + Cstr[i] = '\0'; +} + + + +int_f swapbo_(int_f *i4) +/* + * Returns the big-endian form of a four byte integer + */ +{ + int i=1; + + if (*(char*)&i) { + + int_f swapped; + +#define swap_int4(src, dst) \ + ((char *)&(dst))[0] = ((char *) &(src))[3];\ + ((char *)&(dst))[1] = ((char *) &(src))[2];\ + ((char *)&(dst))[2] = ((char *) &(src))[1];\ + ((char *)&(dst))[3] = ((char *) &(src))[0]; + + swap_int4(*i4,swapped); + return swapped; + } else + return *i4; + +} diff --git a/src/staden/FtoC.h b/src/staden/FtoC.h new file mode 100644 index 0000000..7b301bb --- /dev/null +++ b/src/staden/FtoC.h @@ -0,0 +1,41 @@ +/* + Title: FtoC + + File: FtoC.h + Purpose: FORTRAN-C string conversion routines + Last update: Mon Jun 18 1990 +*/ + +#ifndef _FTOC_H_ +#define _FTOC_H_ + +#include "fort.h" + + +extern void Cstr2Fstr(char *Cstr, + char *Fstr, int_fl Flen); +/* + Copy a '\0' terminated C string to a Fortran string, blank padding + if needed and ignoring excess C characters if needed. + + This function works if the strings are distinct or coincident, but + not if they overlap in any other way. +*/ + + +extern void Fstr2Cstr(char *Fstr, int_fl Flen, + char *Cstr, int_fl Clen); +/* + Copy the significant characters of a blank padded Fortran string + to a '\0' terminated C string, ignoring excess characters. + + This function works if the strings are distinct or coincident, but + not if they overlap in any other way. +*/ + +extern int_f swapbo_(int_f *i4); +/* + * Returns the big-endian form of a four byte integer + */ + +#endif /*_FTOC_H_*/ diff --git a/src/staden/README b/src/staden/README new file mode 100644 index 0000000..2a4ce75 --- /dev/null +++ b/src/staden/README @@ -0,0 +1,37 @@ +The staden suite of sequence analysis programs for the SUN/X11 +-------------------------------------------------------------- + +The contents of this directory should be (at least): +bin executable sequence analysis programs +help helpfiles used by these programs +staden.l manual page +staden.login csh initialisations +staden.profile sh initialisations +tables tables used by the programs + +Installers should first print out a copy of the manual page. +This describes the current state of the software and how to +invoke the initialisations. The manual page should be placed +somewhere where `man' can find it. + +The directory structure should be position independent. It's +location, however, must be encoded in the environment +variable $STADENROOT. This is described in the man page. + + +xdap - Sequence assembly program +-------------------------------- + +This is the source directory for the dap and xdap sequence assembly +programmes, and convert_project. As well as the sources you should +find a makefile and a default resources file "Xdap". + +The "xdap" application can be constructed by simply typing "make xdap", +and installed by typing "make installx" As xdap requires objects from +the "staden" and "ted" directories, it should be made after the staden +package proper and the trace editor have been made. + +The default X resource file "Xdap" files is found in +$STADENROOT/tables, and the environment variable XFILESEARCHPATH is set +in staden.login to refer to it. This file could also be placed in the +"/usr/lib/X11/app-defaults" directory. diff --git a/src/staden/README0 b/src/staden/README0 new file mode 100644 index 0000000..4af2c4e --- /dev/null +++ b/src/staden/README0 @@ -0,0 +1,25 @@ +The staden suite of sequence analysis programs for the SUN/X11 +-------------------------------------------------------------- + +This is the source directory for the staden suite of programs. +This file describes what its contents are, how to make it and +how to install it. The companion file `README' is to be read +by those who have just installed the suite. + +The contents of this directory should be (at least): + The executable sequence analysis programs +*.f, *.c *.h The program sources +help helpfiles used by these programs +makefile +staden.l manual page +staden.login csh initialisations +staden.profile sh initialisations +tables tables used by the programs + +makefile +-------- +The makefile needs the macro `SRCDIR' setting before it runs. +There are commands to make individual programs (e.g. `make xpip'), +all the `normal' xterm programs (`make nprogs'), all the X programs +(`make xprogs'), all the programs (`make all') and to install +everything (`make install') and to install bits. diff --git a/src/staden/Sheet.c b/src/staden/Sheet.c new file mode 100644 index 0000000..fa07b27 --- /dev/null +++ b/src/staden/Sheet.c @@ -0,0 +1,1222 @@ +#include + +/* $XConsortium: Sheet.c,v 1.2 88/10/25 17:40:25 swick Exp $ */ +/* Copyright Massachusetts Institute of Technology 1987, 1988 */ + +#include +#include +#include "SheetP.h" + + +#include +#include + +/* ---- Forward declarations ---- */ + + +static void Initialize(Widget request, Widget new, + ArgList args, Cardinal *num_args); +static void Destroy(Widget w); +static void Redisplay(Widget w, XEvent *event, Region region); +static void Resize(Widget w); +static void InputAction(Widget w, XEvent *event, + String *params,Cardinal *num_params); +static Boolean SetValues(Widget current, Widget request, Widget new, + ArgList args, Cardinal *num_args); + + +static void destroy_array(sheet_array a); +static sheet_array create_array (int r, int c, size_t s); +static void move_array (sheet_array a, sheet_array b); +static void extend_array (sheet_array *a, int r, int c); +static char *get_array_element(sheet_array a, int r, int c); + + +/* ---- Private data ---- */ + + +static XtResource resources[] = { +#define offset(field) XtOffset(SheetWidget, field) + /* {name, class, type, size, offset, default_type, default_addr}, */ + { XtNcallback, XtCCallback, XtRCallback, (Cardinal)sizeof(XtCallbackList), + offset(sheet.input_callback), XtRCallback, NULL }, + { XtNexposeCallback, XtCCallback, XtRCallback, + (Cardinal)sizeof(XtCallbackList), + offset(sheet.expose_callback), XtRCallback, NULL }, + { XtNfont, XtCFont, XtRFontStruct, (Cardinal)sizeof(XFontStruct*), + offset(sheet.font), XtRString, XtDefaultFont }, + { XtNheight, XtCHeight, XtRDimension, (Cardinal)sizeof(Dimension), + offset(core.height), XtRImmediate, (caddr_t)1}, + { XtNwidth, XtCWidth, XtRDimension, (Cardinal)sizeof(Dimension), + offset(core.width), XtRImmediate, (caddr_t)1}, + { XtNresizeCallback, XtCCallback, XtRCallback, + (Cardinal)sizeof(XtCallbackList), + offset(sheet.resize_callback), XtRCallback, NULL }, + { XtNrows, XtCHeight, XtRDimension, (Cardinal)sizeof(Dimension), + offset(sheet.rows), XtRImmediate, (caddr_t)10}, + { XtNcolumns, XtCWidth, XtRDimension, (Cardinal)sizeof(Dimension), + offset(sheet.columns), XtRImmediate, (caddr_t)80}, + { XtNleftMargin, XtCMargin, XtRDimension, (Cardinal)sizeof(Dimension), + offset(sheet.margin.left), XtRImmediate, (caddr_t)4}, + { XtNrightMargin, XtCMargin, XtRDimension, (Cardinal)sizeof(Dimension), + offset(sheet.margin.right), XtRImmediate, (caddr_t)4}, + { XtNtopMargin, XtCMargin, XtRDimension, (Cardinal)sizeof(Dimension), + offset(sheet.margin.top), XtRImmediate, (caddr_t)4}, + { XtNbottomMargin, XtCMargin, XtRDimension, (Cardinal)sizeof(Dimension), + offset(sheet.margin.bottom), XtRImmediate, (caddr_t)4}, + {XtNforeground, XtCForeground, XtRPixel, (Cardinal)sizeof (Pixel), + offset(sheet.foreground), XtRString, XtDefaultForeground}, + {XtNbackground, XtCBackground, XtRPixel, (Cardinal)sizeof (Pixel), + offset(sheet.background), XtRString, XtDefaultBackground}, + {XtNcursor, XtCCursor, XtRCursor, (Cardinal)sizeof(Cursor), + offset(simple.cursor), XtRString, "xterm"}, + {XtNdisplayCursor, XtCOutput, XtRBoolean, (Cardinal)sizeof(Boolean), + offset(sheet.display_cursor), XtRImmediate, (caddr_t)False}, + {XtNcursorRow, XtCWidth, XtRDimension, (Cardinal)sizeof(Dimension), + offset(sheet.cursor_row), XtRImmediate, (caddr_t)0}, + {XtNcursorColumn, XtCHeight, XtRDimension, (Cardinal)sizeof(Dimension), + offset(sheet.cursor_column), XtRImmediate, (caddr_t)0}, +#undef offset +}; + + +static XtActionsRec actions[] = +{ + /* {name, procedure}, */ + {"input", InputAction}, +}; + + +static char translations[] = +": input() \n\ + : input() \ +"; + + +SheetClassRec sheetClassRec = { + { /* core fields initial values */ + /* superclass */ (WidgetClass) &simpleClassRec, + /* class_name */ "Sheet", + /* widget_size */ (Cardinal)sizeof(SheetRec), + /* class_initialize */ NULL, + /* class_part_initialize */ NULL, + /* class_inited */ FALSE, + /* initialize */ Initialize, + /* initialize_hook */ NULL, + /* realize */ XtInheritRealize, + /* actions */ actions, + /* num_actions */ XtNumber(actions), + /* resources */ resources, + /* num_resources */ XtNumber(resources), + /* xrm_class */ NULLQUARK, + /* compress_motion */ TRUE, + /* compress_exposure */ XtExposeNoCompress, + /* compress_exposure XtExposeCompressMaximal, */ + /* compress_enterleave */ TRUE, + /* visible_interest */ FALSE, + /* destroy */ Destroy, + /* resize */ Resize, + /* expose */ Redisplay, + /* set_values */ SetValues, + /* set_values_hook */ NULL, + /* set_values_almost */ XtInheritSetValuesAlmost, + /* get_values_hook */ NULL, + /* accept_focus */ NULL, + /* version */ XtVersion, + /* callback_private */ NULL, + /* tm_table */ translations, + /* query_geometry */ XtInheritQueryGeometry, + /* display_accelerator */ XtInheritDisplayAccelerator, + /* extension */ NULL + }, + { /* Simple class initial values */ + /* change_sensitive */ XtInheritChangeSensitive + }, + { /* Sheet class initial values */ + /* empty */ 0 + } +}; + +WidgetClass sheetWidgetClass = (WidgetClass)&sheetClassRec; + + + + +/* ---- Private procedures --- */ +int binary_op(int src, int dst, int op) +{ + switch (op & HOP_MASK) { + case 0: return 0; + case 1: return ~ (src | dst); + case 2: return ~src & dst; + case 3: return ~src; + case 4: return src & ~dst; + case 5: return ~dst; + case 6: return src ^ dst; + case 7: return ~(src & dst); + case 8: return src & dst; + case 9: return ~src ^ ~dst; + case 10: return dst; + case 11: return ~src|dst; + case 12: return src; + case 13: return src|~dst; + case 14: return src|dst; + case 15: return 1; + } +} + +#define GET_ARRAY_CELL(A,R,C)\ + ( &A->base[(R * A->cols + C)*A->size] ) + +static void destroy_array(sheet_array a) +{ + XtFree (a->base); + XtFree ((char *)a); +} + +static sheet_array create_array (int r, int c, size_t s) +{ + sheet_array new = (sheet_array) XtCalloc (1,sizeof(sheet_array_struct)); + if (new != NULL) { + new->base = (char *) XtCalloc (r*c,s); + if (new->base == NULL) { + XtFree ((char *)new); + new = NULL; + } else { + new->rows = r; + new->cols = c; + new->size = s; + } + } + return new; +} + +#define min(A,B) ((A < B) ? A : B) +static void move_array (sheet_array a, sheet_array b) +{ + size_t r,c; + int i; + + c = min (a->cols*a->size, b->cols*b->size); + r = min (a->rows, b->rows); + for (i=0; irows) + ?(*a)->rows + :(r<=(*a)->rows*2) + ?(*a)->rows*2 + :r+EXTEND_ROWS_GUESS; + if (newr > SHEET_MAX_ROWS) newr= SHEET_MAX_ROWS; + + newc = (c<=(*a)->cols) + ?(*a)->cols + :(c<=(*a)->cols*2) + ?(*a)->cols*2 + :r+EXTEND_COLS_GUESS; + if (newc > SHEET_MAX_COLS) newc= SHEET_MAX_COLS; + + if (newr!=(*a)->rows || newc!=(*a)->cols) { + b = create_array(newr,newc,(*a)->size); + move_array(*a,b); + destroy_array(*a); + *a = b; + } +} + +/* +static char * get_array_element(sheet_array a, int r, int c) +{ + if (r < 0 || c < 0) + return NULL; + if (a->rows > r || a->cols > c) + return NULL; + + return GET_ARRAY_CELL(a,r,c); +} +*/ + + +/* ---- Private Routines ---- */ +#define fontWidth(F) ((F)->max_bounds.width) +#define fontHeight(F) ((F)->max_bounds.ascent + (F)->max_bounds.descent) +#define COL_TO_PIXEL(W,C) \ + ((W)->sheet.font->max_bounds.width * (C) + (W)->sheet.margin.left) +#define ROW_TO_BASELINE_PIXEL(W,R) \ + (fontHeight((W)->sheet.font) * (R) + (W)->sheet.margin.top + (W)->sheet.font->max_bounds.ascent) +#define ROW_TO_PIXEL(W,R) \ + (fontHeight((W)->sheet.font) * ((R)+1) + (W)->sheet.margin.top) +#define PIXEL_TO_COL(W,P) \ + (((long)(P) - (long)(W)->sheet.margin.left) / (long)(W)->sheet.font->max_bounds.width) +#define PIXEL_WIDTH_TO_COLS(W,P) \ + (((long)(P) - (long)(W)->sheet.margin.left - (long)(W)->sheet.margin.right) / (long)(W)->sheet.font->max_bounds.width) +#define COLS_TO_PIXEL_WIDTH(W,C) \ + (((C) * (W)->sheet.font->max_bounds.width) + (W)->sheet.margin.left + (W)->sheet.margin.right) +#define BASELINE_PIXEL_TO_ROW(W,P) \ + (((long)(P) - (long)(W)->sheet.margin.top - (long)(W)->sheet.font->max_bounds.ascent) / (long)fontHeight((W)->sheet.font) ) +#define PIXEL_TO_ROW(W,P) \ + (((long)(P) - (long)(W)->sheet.margin.top) / (long)fontHeight((W)->sheet.font) ) +#define PIXEL_HEIGHT_TO_ROWS(W,P) \ + (((long)(P) - (long)(W)->sheet.margin.top - (long)(W)->sheet.margin.bottom) / (long)fontHeight((W)->sheet.font) ) +#define ROWS_TO_PIXEL_HEIGHT(W,R) \ + (((R) * fontHeight((W)->sheet.font)) + (W)->sheet.margin.top + (W)->sheet.margin.bottom) +#define FONT_WIDTH(W) (fontWidth((W)->sheet.font)) +#define FONT_HEIGHT(W) (fontHeight((W)->sheet.font)) + +static void setGC(SheetWidget sw, GC gc, sheet_ink ink_base) +{ + if (ink_base->sh & sh_inverse) { + if (ink_base->sh&sh_bg) + XSetForeground(XtDisplay(sw),gc,ink_base->bg); + else + XSetForeground(XtDisplay(sw),gc,sw->sheet.background); + if (ink_base->sh&sh_fg) + XSetBackground(XtDisplay(sw),gc,ink_base->fg); + else + XSetBackground(XtDisplay(sw),gc,sw->sheet.foreground); + } else { + if (ink_base->sh&sh_fg) + XSetForeground(XtDisplay(sw),gc,ink_base->fg); + else + XSetForeground(XtDisplay(sw),gc,sw->sheet.foreground); + if (ink_base->sh&sh_bg) + XSetBackground(XtDisplay(sw),gc,ink_base->bg); + else + XSetBackground(XtDisplay(sw),gc,sw->sheet.background); + } + +} + +static void redrawCursor(SheetWidget sw, Boolean draw); + +static void old_repaint(SheetWidget sw, int c, int r, int l, sheet_ink ink, char *s) +{ + /* + int cursorZapped; + + cursorZapped = (sw->sheet.display_cursor && + sw->sheet.cursor_row >= r && + sw->sheet.cursor_row < (r+l) && + sw->sheet.cursor_column == c); + */ + + if (ink->sh==sh_default) { + XDrawImageString( + XtDisplay(sw), + XtWindow(sw), + sw->sheet.normgc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_BASELINE_PIXEL(sw,r), + s, + l); + } else { + if (ink->sh & sh_light) { + if (DisplayPlanes(XtDisplay(sw),DefaultScreen(XtDisplay(sw)))==1){ + XFillRectangle( + XtDisplay(sw), + XtWindow(sw), + sw->sheet.whitegc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_PIXEL(sw,r-1), + FONT_WIDTH(sw) * l, + FONT_HEIGHT(sw) + ); + setGC(sw, sw->sheet.greygc, ink); + XDrawString( + XtDisplay(sw), + XtWindow(sw), + sw->sheet.greygc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_BASELINE_PIXEL(sw,r), + s, + l); + } else { + sheet_ink_struct my_ink; + my_ink.sh = sh_fg; + my_ink.fg = sw->sheet.light; + setGC(sw, sw->sheet.sparegc, &my_ink); + XDrawImageString( + XtDisplay(sw), + XtWindow(sw), + sw->sheet.sparegc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_BASELINE_PIXEL(sw,r), + s, + l); + } + } else { + if (DisplayPlanes(XtDisplay(sw),DefaultScreen(XtDisplay(sw)))==1){ + XFillRectangle( + XtDisplay(sw), + XtWindow(sw), + sw->sheet.greygc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_PIXEL(sw,r-1), + FONT_WIDTH(sw) * l, + FONT_HEIGHT(sw) + ); + XDrawString( + XtDisplay(sw), + XtWindow(sw), + sw->sheet.normgc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_BASELINE_PIXEL(sw,r), + s, + l); + } else { + setGC(sw, sw->sheet.sparegc, ink); + + + XDrawImageString( + XtDisplay(sw), + XtWindow(sw), + sw->sheet.sparegc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_BASELINE_PIXEL(sw,r), + s, + l); + } + } + if (ink->sh & sh_select || ink->sh & sh_underline) { + setGC(sw, sw->sheet.sparegc, ink); + XDrawLine( + XtDisplay(sw), + XtWindow(sw), + sw->sheet.sparegc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_BASELINE_PIXEL(sw,r), + (int) COL_TO_PIXEL(sw,c+l)-1, + (int) ROW_TO_BASELINE_PIXEL(sw,r) + ); + } + } + + /* + if (cursorZapped) + redrawCursor(sw,True); + */ +} + + + + +static void _repaint_colour(SheetWidget sw, int c, int r, int l, sheet_ink ink, char *s) +{ + sheet_ink_struct my_ink; + my_ink = *ink; + + if (ink->sh & sh_light) { + my_ink.sh = (my_ink.sh | sh_fg) & ~sh_bg; + my_ink.fg = sw->sheet.light; + } + + setGC(sw, sw->sheet.sparegc, &my_ink); + XDrawImageString( + XtDisplay(sw), + XtWindow(sw), + sw->sheet.sparegc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_BASELINE_PIXEL(sw,r), + s, + l); + + if (ink->sh & sh_select || ink->sh & sh_underline) { + XDrawLine( + XtDisplay(sw), + XtWindow(sw), + sw->sheet.sparegc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_BASELINE_PIXEL(sw,r), + (int) COL_TO_PIXEL(sw,c+l)-1, + (int) ROW_TO_BASELINE_PIXEL(sw,r) + ); + } + +} + + +static void _repaint_monochrome(SheetWidget sw, int c, int r, int l, sheet_ink ink, char *s) +{ + + GC fg_gc; + GC bg_gc; + +#define L ( ink->sh & sh_light ) +#define I ( ink->sh & sh_inverse ) +#define BG ( ink->sh & (sh_bg | sh_fg) ) + /* + ** bg_determination + */ + bg_gc = ( I && !L ) ? sw->sheet.normgc : + (I || (!L && BG)) ? sw->sheet.greygc : + sw->sheet.whitegc; + /* + ** fg_determination + */ + fg_gc = ( !I && !L ) ? sw->sheet.normgc : + (!I || (!L && BG)) ? sw->sheet.greygc : + sw->sheet.whitegc; +#undef L +#undef I +#undef BG + + XFillRectangle( + XtDisplay(sw), + XtWindow(sw), + bg_gc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_PIXEL(sw,r-1), + FONT_WIDTH(sw) * l, + FONT_HEIGHT(sw) + ); + XDrawString( + XtDisplay(sw), + XtWindow(sw), + fg_gc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_BASELINE_PIXEL(sw,r), + s, + l); + + if (ink->sh & sh_select || ink->sh & sh_underline) { + setGC(sw, sw->sheet.sparegc, ink); + XDrawLine( + XtDisplay(sw), + XtWindow(sw), + fg_gc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_BASELINE_PIXEL(sw,r), + (int) COL_TO_PIXEL(sw,c+l)-1, + (int) ROW_TO_BASELINE_PIXEL(sw,r) + ); + } +} + + + +static void _repaint(SheetWidget sw, int c, int r, int l, sheet_ink ink, char *s) +{ + if (ink->sh==sh_default) { + XDrawImageString( + XtDisplay(sw), + XtWindow(sw), + sw->sheet.normgc, + (int) COL_TO_PIXEL(sw,c), + (int) ROW_TO_BASELINE_PIXEL(sw,r), + s, + l); + } else { + if (DisplayPlanes(XtDisplay(sw),DefaultScreen(XtDisplay(sw)))==1) + _repaint_monochrome(sw,c,r,l,ink,s); + else + _repaint_colour(sw,c,r,l,ink,s); + } + +} + + +static void redrawCursor(SheetWidget sw, Boolean draw) +{ + XawSheetRow r = sw->sheet.cursor_row; + XawSheetColumn c = sw->sheet.cursor_column; + sheet_ink ink_base = (sheet_ink) GET_ARRAY_CELL(sw->sheet.ink,r,c); + sheet_paper paper_base = (sheet_paper) GET_ARRAY_CELL(sw->sheet.paper,r,c); + sheet_ink_struct ink; + int cursor_was; + + /* check cursor is on screen */ + if (r < 0 || r > sw->sheet.rows-1 || + c < 0 || c > sw->sheet.columns-1 ) return; + + ink.fg = ink_base->fg; + ink.bg = ink_base->bg; + if (draw) + ink.sh = ink_base->sh | sh_inverse; + else + ink.sh = ink_base->sh; + + /* + cursor_was = sw->sheet.display_cursor; + sw->sheet.display_cursor = 0; + */ + _repaint(sw, c, r, 1, &ink, paper_base); + /* + sw->sheet.display_cursor = cursor_was; + */ + +} + +static void repaintText(SheetWidget sw, int c, int r, int l) +{ + sheet_ink ink_base = (sheet_ink) GET_ARRAY_CELL(sw->sheet.ink,r,c); + sheet_ink ink_peek; + sheet_paper paper_base = (sheet_paper) GET_ARRAY_CELL(sw->sheet.paper,r,c); + sheet_paper paper_peek; + XawSheetColumn c_peek; + int i; + + while (l > 0) { + /* find stretch where all hilight the same */ + ink_peek = ink_base; + ink_peek++; + paper_peek = paper_base; + paper_peek++; + c_peek = c; + c_peek++; + i = 1; + l--; +#define implies(A,B) ((B)|!(A)) + while ( (l > 0) && + (ink_peek->sh == ink_base->sh) && + implies(ink_base->sh&sh_fg,ink_peek->fg==ink_base->fg) && + implies(ink_base->sh&sh_bg,ink_peek->bg==ink_base->bg) ) { + ink_peek++; + paper_peek++; + c_peek++; + i++; + l--; + } + + _repaint(sw, c, r, i, ink_base, paper_base); + + paper_base = paper_peek; + ink_base = ink_peek; + c = c_peek; + } + +} + +static void redisplayRegion(Widget w, XRectangle *expose) +{ + SheetWidget sw = (SheetWidget) w; + sheet_ink cell; + + int tlc,brc,c; + int tlr,brr,r; + + tlc = PIXEL_TO_COL(sw,expose->x); + tlr = PIXEL_TO_ROW(sw,expose->y); + brc = PIXEL_TO_COL(sw,expose->x+expose->width-1)+1; + brr = PIXEL_TO_ROW(sw,expose->y+expose->height-1)+1; + if (tlc < 0) tlc = 0; + if (tlr < 0) tlr = 0; + if (brc < 0) brc = 0; + if (brr < 0) brr = 0; + if (tlc >= sw->sheet.columns) tlc = sw->sheet.columns-1; + if (tlr >= sw->sheet.rows) tlr = sw->sheet.rows-1; + if (brc >= sw->sheet.columns) brc = sw->sheet.columns-1; + if (brr >= sw->sheet.rows) brr = sw->sheet.rows-1; + + for (r=tlr;r<=brr;r++) { + repaintText(sw, tlc, r, brc-tlc+1); + } + + if (sw->sheet.display_cursor && + sw->sheet.cursor_row >= tlr && + sw->sheet.cursor_row <= brr && + sw->sheet.cursor_column >= tlc && + sw->sheet.cursor_column <= brc) + { + /* better redraw cursor */ + redrawCursor(sw,True); + } +} + +/* ---- Exported procedures ---- */ + + + +void XawSheetPutText(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l, String s) +/* +** Put plain text +*/ +{ + SheetWidget sw = (SheetWidget) w; + int i; + sheet_ink ink_base; + sheet_paper paper_base; + String sp; + + if (r>=0 && rsheet.rows && + c+l>0 && csheet.columns && + l > 0) { + if (c<0) { l += c; s -= c; c = 0; } + if (c+l>sw->sheet.columns) l = sw->sheet.columns - c; + for ( + i = 0, sp = s, + ink_base = (sheet_ink) GET_ARRAY_CELL(sw->sheet.ink,r,c), + paper_base = (sheet_paper) GET_ARRAY_CELL(sw->sheet.paper,r,c); + i < l; + i++, ink_base++, paper_base++, sp++) { + ink_base->sh = sh_default; + *paper_base = *sp; + } + if (XtIsRealized(w)) { + _repaint(sw, c, r, l, (sheet_ink) GET_ARRAY_CELL(sw->sheet.ink,r,c), s); + + if (sw->sheet.display_cursor && + sw->sheet.cursor_row == r && + sw->sheet.cursor_column >= c && + sw->sheet.cursor_column < c+l) + { + /* better redraw cursor */ + redrawCursor(sw,True); + } + } + } +} + + +void XawSheetPutJazzyText(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l, String s, XawSheetInk *ink_list) +/* +** Put multi-coloured text +*/ +{ + SheetWidget sw = (SheetWidget) w; + int i; + sheet_ink ink_base; + sheet_paper paper_base; + String sp; + + if (r>=0 && rsheet.rows && + c+l>0 && csheet.columns && + l > 0) { + if (c<0) { l += c; s -= c; c = 0; } + if (c+l>sw->sheet.columns) l = sw->sheet.columns - c; + for ( + i = 0, sp = s, + ink_base = (sheet_ink) GET_ARRAY_CELL(sw->sheet.ink,r,c), + paper_base = (sheet_paper) GET_ARRAY_CELL(sw->sheet.paper,r,c); + i < l; + i++, ink_base++, ink_list++, paper_base++, sp++) { + ink_base->fg = ink_list->fg; + ink_base->bg = ink_list->bg; + ink_base->sh = ink_list->sh; + *paper_base = *sp; + } + if (XtIsRealized(w)) { + repaintText(sw, c, r, l); + + if (sw->sheet.display_cursor && + sw->sheet.cursor_row == r && + sw->sheet.cursor_column >= c && + sw->sheet.cursor_column < c+l) + { + /* better redraw cursor */ + redrawCursor(sw,True); + } + } + } +} + + +void XawSheetPutHilightText(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l, String s) +/* +** Put text using default hilights +*/ +{ + SheetWidget sw = (SheetWidget) w; + int i; + sheet_ink ink_base; + sheet_paper paper_base; + String sp; + + if (r>=0 && rsheet.rows && + c+l>0 && csheet.columns && + l > 0) { + if (c<0) { l += c; s -= c; c = 0; } + if (c+l>sw->sheet.columns) l = sw->sheet.columns - c; + for ( + i = 0, sp = s, + ink_base = (sheet_ink) GET_ARRAY_CELL(sw->sheet.ink,r,c), + paper_base = (sheet_paper) GET_ARRAY_CELL(sw->sheet.paper,r,c); + i < l; + i++, ink_base++, paper_base++, sp++) { + ink_base->sh = sw->sheet.default_sh; + ink_base->fg = sw->sheet.default_fg; + ink_base->bg = sw->sheet.default_bg; + *paper_base = *sp; + } + if (XtIsRealized(w)) { + _repaint(sw, c, r, l, (sheet_ink) GET_ARRAY_CELL(sw->sheet.ink,r,c), s); + if (sw->sheet.display_cursor && + sw->sheet.cursor_row == r && + sw->sheet.cursor_column >= c && + sw->sheet.cursor_column < c+l) + { + /* better redraw cursor */ + redrawCursor(sw,True); + } + } + } +} + +void XawSheetHilightText(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l, Pixel fg, Pixel bg, XawSheetHilight h) +/* +** Hilight already draw text +*/ +{ +SheetWidget sw = (SheetWidget) w; +sheet_ink ink_base; +sheet_paper paper_base; + +/* +Hilights currently supported: + + sh_default yes + sh_fg yes + sh_bg yes + sh_underline yes + sh_inverse yes + sh_light no + sh_tick no + sh_bold no + sh_italic no +*/ + + if (r>=0 && rsheet.rows && + c+l>0 && csheet.columns && + l > 0) { + int i; + + if (c<0) { l += c; c = 0; } + if (c+l>sw->sheet.columns) l = sw->sheet.columns - c; + for ( + i = 0, + ink_base = (sheet_ink) GET_ARRAY_CELL(sw->sheet.ink,r,c), + paper_base = (sheet_paper) GET_ARRAY_CELL(sw->sheet.paper,r,c); + i < l; + i++, ink_base++, paper_base++) + { + if (h==sh_default) { + ink_base->sh = sh_default; + } else { + if (h & sh_fg) ink_base->fg = fg; + if (h & sh_bg) ink_base->bg = bg; + ink_base->sh |= h; + } + } + repaintText(sw, (int)c, (int)r, (int)l); + } +} + +void XawSheetUnhilightText(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l, Pixel fg, Pixel bg, XawSheetHilight h) +/* +** Remove hilighting from text +*/ +{ +SheetWidget sw = (SheetWidget) w; +sheet_ink ink_base; +sheet_paper paper_base; + + + if (r>=0 && rsheet.rows && + c+l>0 && csheet.columns && + l > 0) { + int i; + + if (c<0) { l += c; c = 0; } + if (c+l>sw->sheet.columns) l = sw->sheet.columns - c; + for ( + i = 0, + ink_base = (sheet_ink) GET_ARRAY_CELL(sw->sheet.ink,r,c), + paper_base = (sheet_paper) GET_ARRAY_CELL(sw->sheet.paper,r,c); + i < l; + i++, ink_base++, paper_base++) + { + if (h==sh_default) { + } else { + if (h & sh_fg) ink_base->fg = fg; + if (h & sh_bg) ink_base->bg = bg; + ink_base->sh &= !h&sh_mask; + } + } + repaintText(sw, (int)c, (int)r, (int)l); + } +} + +void XawSheetOpHilightText(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l, XawSheetHilight h, int op) +/* +** Perform boolean operations on text +*/ +{ +SheetWidget sw = (SheetWidget) w; +sheet_ink ink_base; +sheet_paper paper_base; + + + if (r>=0 && rsheet.rows && + c+l>0 && csheet.columns && + l > 0) { + int i; + + if (c<0) { l += c; c = 0; } + if (c+l>sw->sheet.columns) l = sw->sheet.columns - c; + for ( + i = 0, + ink_base = (sheet_ink) GET_ARRAY_CELL(sw->sheet.ink,r,c), + paper_base = (sheet_paper) GET_ARRAY_CELL(sw->sheet.paper,r,c); + i < l; + i++, ink_base++, paper_base++) + { + ink_base->sh = binary_op(h,ink_base->sh,op)&sh_mask; + } + repaintText(sw, (int)c, (int)r, (int)l); + if (sw->sheet.display_cursor && + sw->sheet.cursor_row == r && + sw->sheet.cursor_column >= c && + sw->sheet.cursor_column < c+l) + { + /* better redraw cursor */ + redrawCursor(sw,True); + } + + } +} + +void XawSheetPositionCursor(Widget w, XawSheetColumn c, XawSheetRow r) +{ + SheetWidget sw = (SheetWidget) w; + if (XtIsRealized(w) && sw->sheet.display_cursor) + redrawCursor(sw,False); + sw->sheet.cursor_column = c; + sw->sheet.cursor_row = r; + if (XtIsRealized(w) && sw->sheet.display_cursor) + redrawCursor(sw,True); +} + +void XawSheetDisplayCursor(Widget w, Boolean b) +{ + SheetWidget sw = (SheetWidget) w; + if (sw->sheet.display_cursor^b) {/*state change*/ + sw->sheet.display_cursor = b; + if (XtIsRealized(w)) redrawCursor(sw, b); + } +} + +Pixel XawSheetColourNameToPixel(Widget w, String c) +{ + XColor rgb_db_def, hardware_def; + Colormap cmap; + Status s; + + cmap = DefaultColormap(XtDisplay(w),DefaultScreen(XtDisplay(w))); + s = XAllocNamedColor(XtDisplay(w), cmap, c, &rgb_db_def, &hardware_def); + + return hardware_def.pixel; +} + + + +void XawSheetSetHilight(Widget w, Pixel fg, Pixel bg, XawSheetHilight h) +{ + SheetWidget sw = (SheetWidget) w; + + if (h & sh_fg) sw->sheet.default_fg = fg; + if (h & sh_bg) sw->sheet.default_bg = bg; + sw->sheet.default_sh = h; + +} + +void XawSheetSetRows(Widget w, XawSheetRow r) +{ + SheetWidget sw = (SheetWidget) w; +} +void XawSheetSetColumns(Widget w, XawSheetColumn c) +{ + SheetWidget sw = (SheetWidget) w; +} +void XawSheetDeleteRow(Widget w, XawSheetRow r) +{ + SheetWidget sw = (SheetWidget) w; +} +void XawSheetDeleteColumn(Widget w, XawSheetColumn c) +{ + SheetWidget sw = (SheetWidget) w; +} +void XawSheetInsertRow(Widget w, XawSheetRow r) +{ + SheetWidget sw = (SheetWidget) w; +} +void XawSheetInsertColumn(Widget w, XawSheetColumn c) +{ + SheetWidget sw = (SheetWidget) w; +} +void XawSheetInsert(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l) +{ + SheetWidget sw = (SheetWidget) w; +} +void XawSheetDelete(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l) +{ + SheetWidget sw = (SheetWidget) w; +} +void XawSheetClearSheet(Widget w) +{ + SheetWidget sw = (SheetWidget) w; +} + + + +/* ---- Private procedures -- Class Methods ---- */ +static Pixel ColourNameToPixel(Widget w, String c) +{ + XColor rgb_db_def, hardware_def; + Colormap cmap; + Status s; + + cmap = DefaultColormap(XtDisplay(w),DefaultScreen(XtDisplay(w))); + s = XAllocNamedColor(XtDisplay(w), cmap, c, &rgb_db_def, &hardware_def); + + return hardware_def.pixel; +} + + +static void Initialize(Widget request, Widget new, + ArgList args, Cardinal *num_args) +{ SheetWidget sw = (SheetWidget)new; + Display *display; + int screen; + + display = XtDisplay(request); + screen = DefaultScreen(display); + + if (DisplayPlanes(display,screen)==1) + { /* We are on a one-plane monochrome display. + Use dashes to make lines distinguishable. + */ + + } + else + { /* Some sort of multi-plane display. + */ + + } + sw->sheet.paper = create_array(sw->sheet.rows,sw->sheet.columns, sizeof(sheet_paper_struct)); + sw->sheet.ink = create_array(sw->sheet.rows,sw->sheet.columns, sizeof(sheet_ink_struct)); + sw->sheet.cursor.row = 0; + sw->sheet.cursor.column = 0; + sw->sheet.cursor.visible = False; + sw->sheet.default_sh = sh_default; + + /* get font details */ + + sw->sheet.width_in_pixels = COLS_TO_PIXEL_WIDTH(sw,sw->sheet.columns); + sw->sheet.height_in_pixels = ROWS_TO_PIXEL_HEIGHT(sw,sw->sheet.rows); + + sw->core.width = sw->sheet.width_in_pixels; + sw->core.height = sw->sheet.height_in_pixels; + + /* GCs and things */ + { + XtGCMask valuemask = (GCFont | + GCGraphicsExposures | GCForeground | GCBackground ); + XGCValues values; + + values.font = sw->sheet.font->fid; + values.graphics_exposures = (Bool) FALSE; + + values.foreground = sw->sheet.foreground; + values.background = sw->sheet.background; + sw->sheet.normgc = XtGetGC((Widget)sw, valuemask, &values); + + values.foreground = sw->sheet.background; + values.background = sw->sheet.background; + sw->sheet.sparegc = XtGetGC((Widget)sw, valuemask, &values); + + values.foreground = sw->sheet.foreground; + values.background = sw->sheet.background; + sw->sheet.greygc = XCreateGC(XtDisplayOfObject((Widget)sw), + RootWindowOfScreen(XtScreenOfObject( + (Widget)sw)), + valuemask, &values); + + values.foreground = sw->sheet.background; + values.background = sw->sheet.foreground; + sw->sheet.whitegc = XtGetGC((Widget)sw, valuemask, &values); + + if (DisplayPlanes(XtDisplay(sw),DefaultScreen(XtDisplay(sw)))==1){ +#define grey_width 2 +#define grey_height 2 + static char grey_bits[] = { 0x01 , 0x02 }; + + sw->sheet.grey_stipple = + XCreateBitmapFromData(XtDisplay(sw), + RootWindowOfScreen(XtScreenOfObject( + (Widget)sw)), + grey_bits, + grey_width, + grey_height); + XSetFillStyle(XtDisplay(sw),sw->sheet.greygc,FillOpaqueStippled); + XSetStipple(XtDisplay(sw),sw->sheet.greygc,sw->sheet.grey_stipple); + } else { + sw->sheet.light = ColourNameToPixel((Widget)sw,"lightGray"); + } + + } + + +} + + + +static void Destroy (Widget w) +{ + SheetWidget sw = (SheetWidget) w; + + destroy_array(sw->sheet.paper); + destroy_array(sw->sheet.ink); +} + +/* ARGSUSED */ +static void Redisplay(Widget w, XEvent *event, Region region) +{ + SheetWidget sw = (SheetWidget) w; + XRectangle expose, cursor; + + if (!XtIsRealized(w)) + return; + + if (event->type == Expose) { + expose.x = event->xexpose.x; + expose.y = event->xexpose.y; + expose.width = event->xexpose.width; + expose.height = event->xexpose.height; + } + else { /* Graphics Expose. */ + expose.x = event->xgraphicsexpose.x; + expose.y = event->xgraphicsexpose.y; + expose.width = event->xgraphicsexpose.width; + expose.height = event->xgraphicsexpose.height; + } + + redisplayRegion((Widget)sw, &expose); +} + + + + +static void Resize(Widget w) +{ + SheetWidget sw = (SheetWidget) w; + int newRows, newCols; + + newRows = PIXEL_HEIGHT_TO_ROWS(sw,sw->core.height); + newCols = PIXEL_WIDTH_TO_COLS(sw,sw->core.width); + if (newRows > SHEET_MAX_ROWS) { + newRows = SHEET_MAX_ROWS; + sw->sheet.height_in_pixels = ROWS_TO_PIXEL_HEIGHT(sw,newRows); + /* + sw->core.height = sw->sheet.height_in_pixels; + */ + } + sw->sheet.rows = newRows; + + if (newCols > SHEET_MAX_COLS) { + newCols = SHEET_MAX_COLS; + sw->sheet.width_in_pixels = COLS_TO_PIXEL_WIDTH(sw,newCols); + /* + sw->core.width = sw->sheet.width_in_pixels; + */ + } + sw->sheet.columns = newCols; + + extend_array ( + &sw->sheet.paper, + newRows, + newCols + ); + extend_array ( + &sw->sheet.ink, + newRows, + newCols + ); +} + + +static void InputAction(Widget w, XEvent *event, + String *params,Cardinal *num_params) +{ + SheetWidget sw = (SheetWidget) w; +} + + +static Boolean SetValues(Widget current, Widget request, Widget new, + ArgList args, Cardinal *num_args) +{ + SheetWidget oldsw = (SheetWidget) current; + SheetWidget newsw = (SheetWidget) new; + Boolean redisplay; + Boolean resize; + + redisplay = FALSE; + resize = FALSE; + if (oldsw->sheet.rows != newsw->sheet.rows) { + if (newsw->sheet.rows < 1 || + newsw->sheet.rows >= SHEET_MAX_ROWS) + newsw->sheet.rows = oldsw->sheet.rows; + if (oldsw->sheet.rows != newsw->sheet.rows) { + redisplay = TRUE; + resize = TRUE; + newsw->sheet.height_in_pixels = ROWS_TO_PIXEL_HEIGHT(newsw,newsw->sheet.rows); + newsw->core.height = newsw->sheet.height_in_pixels; + } + } + if (oldsw->sheet.columns != newsw->sheet.columns) { + if (newsw->sheet.columns < 1 || + newsw->sheet.columns >= SHEET_MAX_COLS) + newsw->sheet.columns = oldsw->sheet.columns; + if (oldsw->sheet.columns != newsw->sheet.columns) { + redisplay = TRUE; + resize = TRUE; + newsw->sheet.width_in_pixels = COLS_TO_PIXEL_WIDTH(newsw,newsw->sheet.columns); + newsw->core.width = newsw->sheet.width_in_pixels; + } + } + + if (resize) { + extend_array ( + &newsw->sheet.paper, + newsw->sheet.rows, + newsw->sheet.columns + ); + extend_array ( + &newsw->sheet.ink, + newsw->sheet.rows, + newsw->sheet.columns + ); + } + + return (redisplay && XtIsRealized((Widget)oldsw)); + +} + diff --git a/src/staden/Sheet.h b/src/staden/Sheet.h new file mode 100644 index 0000000..a938e2a --- /dev/null +++ b/src/staden/Sheet.h @@ -0,0 +1,136 @@ +#include + +/* $XConsortium: Sheet.h,v 1.2 88/10/25 17:22:09 swick Exp $ */ +/* Copyright Massachusetts Institute of Technology 1987, 1988 */ + +#ifndef _Sheet_h +#define _Sheet_h + +/**************************************************************** + * + * Sheet widget + * + ****************************************************************/ + +#include + +/* Resources: + + Name Class RepType Default Value + ---- ----- ------- ------------- + background Background Pixel XtDefaultBackground + border BorderColor Pixel XtDefaultForeground + borderWidth BorderWidth Dimension 1 + bottomMargin Margin Dimension 4 + callback Callback Callback NULL + columns Width Dimension 80 + cursor Cursor Cursor None + cursorColumn Width Dimension 0 + cursorRow Height Dimension 0 + destroyCallback Callback Pointer NULL + displayCursor Output Boolean False + exposeCallback Callback Callback NULL + font Font XFontStruct* XtDefaultFont + foreground Foreground Pixel XtDefaultForeground + height Height Dimension 1 + insensitiveBorder Insensitive Pixmap Gray + leftMargin Margin Dimension 4 + mappedWhenManaged MappedWhenManaged Boolean True + resizeCallback Callback Callback NULL + rightMargin Margin Dimension 4 + rows Height Dimension 10 + sensitive Sensitive Boolean True + topMargin Margin Dimension 4 + width Width Dimension 1 + +*/ + +/* define any special resource names here that are not in */ + +#define XtNsheetResource "sheetResource" +#define XtCSheetResource "SheetResource" + +#define XtNdimBackground "dimBackground" +#define XtNexposeCallback "exposeCallback" +#define XtNresizeCallback "resizeCallback" +#define XtNrows "rows" +#define XtNcolumns "columns" +#define XtNleftMargin "leftMargin" +#define XtNrightMargin "rightMargin" +#define XtNtopMargin "topMargin" +#define XtNbottomMargin "bottomMargin" +#define XtNdisplayCursor "displayCursor" +#define XtNcursorRow "cursorRow" +#define XtNcursorColumn "cursorColumn" + +#define XtCOutput "Output" + + +/* declare specific SheetWidget class and instance datatypes */ + +typedef struct _SheetClassRec *SheetWidgetClass; +typedef struct _SheetRec *SheetWidget; +typedef Dimension XawSheetRow; +typedef Dimension XawSheetColumn; +typedef long XawSheetHilight; +typedef struct { + Pixel fg; + Pixel bg; + XawSheetHilight sh; +} XawSheetInk; + +/* hilights available */ +#define sh_default (0L) +#define sh_fg (1L<<0) +#define sh_bg (1L<<1) +#define sh_underline (1L<<2) +#define sh_inverse (1L<<3) +#define sh_light (1L<<4) +#define sh_tick (1L<<5) +#define sh_bold (1L<<6) +#define sh_italic (1L<<7) +#define sh_select (1L<<8) +#define sh_mask ((1L<<9) - 1) + +/* hilight operations */ +#define HOP_MASK 0xF +#define HOP_SRC 0xC +#define HOP_DST 0xA +#define HOP_SET 0xD +#define HOP_CLR 0x2 +#define HOP_TOG 0x6 +#define HOP_AND(S,D) ((S & D) & HOP_MASK) +#define HOP_OR(S,D) ((S | D) & HOP_MASK) +#define HOP_NOT(S) ((!S) & HOP_MASK) + +#define SHEET_MAX_COLS 512 +#define SHEET_MAX_ROWS 1024 + +/* declare the class constant */ + +extern WidgetClass sheetWidgetClass; + + +/* ---- Exported procedures ---- */ + +extern void XawSheetPositionCursor(Widget w, XawSheetColumn c, XawSheetRow r); +extern void XawSheetDisplayCursor(Widget w, Boolean B); +extern void XawSheetPutText(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l, String s); +extern void XawSheetSetRows(Widget w, XawSheetRow r); +extern void XawSheetSetColumns(Widget w, XawSheetColumn c); +extern void XawSheetDeleteRow(Widget w, XawSheetRow r); +extern void XawSheetDeleteColumn(Widget w, XawSheetColumn c); +extern void XawSheetInsertRow(Widget w, XawSheetRow r); +extern void XawSheetInsertColumn(Widget w, XawSheetColumn c); +extern void XawSheetInsert(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l); +extern void XawSheetDelete(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l); +extern Pixel XawSheetColourNameToPixel(Widget w, String c); +extern void XawSheetSetHilight(Widget w, Pixel fg, Pixel bg, XawSheetHilight h); +extern void XawSheetPutHilightText(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l, String s); +extern void XawSheetHilightText(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l, Pixel fg, Pixel bg, XawSheetHilight h); +extern void XawSheetUnhilightText(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l, Pixel fg, Pixel bg, XawSheetHilight h); +extern void XawSheetOpHilightText(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l, XawSheetHilight h, int op); +extern void XawSheetClearSheet(Widget w); +extern void XawSheetPutJazzyText(Widget w, XawSheetColumn c, XawSheetRow r, Dimension l, String s, XawSheetInk *ink_list); + +#endif /* _Sheet_h */ diff --git a/src/staden/SheetP.h b/src/staden/SheetP.h new file mode 100644 index 0000000..4c51e75 --- /dev/null +++ b/src/staden/SheetP.h @@ -0,0 +1,103 @@ +#include + +/* $XConsortium: SheetP.h,v 1.2 88/10/25 17:37:59 swick Exp $ */ +/* Copyright Massachusetts Institute of Technology 1987, 1988 */ + +#ifndef _SheetP_h +#define _SheetP_h + +#include "Sheet.h" +/* include superclass private header file */ +#include + + +/* define unique representation types not found in */ + +#define XtRSheetResource "SheetResource" + +typedef struct { + int empty; +} SheetClassPart; + +typedef struct _SheetClassRec { + CoreClassPart core_class; + SimpleClassPart simple_class; + SheetClassPart sheet_class; +} SheetClassRec; + +extern SheetClassRec sheetClassRec; + +/* +typedef struct { + Pixel fg; + Pixel bg; + XawSheetHilight sh; + char c; + char pad[3]; +} *sheet_cell, sheet_cell_struct; +*/ + +typedef struct { + XawSheetRow rows; + XawSheetColumn cols; + char *base; + size_t size; +} *sheet_array, sheet_array_struct; + +typedef struct { + Pixel fg; + Pixel bg; + XawSheetHilight sh; +} *sheet_ink, sheet_ink_struct; + +typedef char *sheet_paper, sheet_paper_struct; + +typedef struct { + XawSheetRow row; + XawSheetColumn column; + Boolean visible; +} sheet_cursor; + +typedef struct { + Dimension left,right,top,bottom; +} XawSheetMargin; + + +typedef struct { + /* resources */ + Pixel foreground; + Pixel background; + XFontStruct* font; + XtCallbackList expose_callback; + XtCallbackList resize_callback; + XtCallbackList input_callback; + XawSheetMargin margin; + XawSheetRow rows; + XawSheetColumn columns; + Boolean display_cursor; + XawSheetRow cursor_row; + XawSheetColumn cursor_column; + /* private state */ + sheet_array paper; + sheet_array ink; + sheet_cursor cursor; + int width_in_pixels; + int height_in_pixels; + GC normgc; + GC greygc; + GC whitegc; + GC sparegc; + Pixel default_fg; + Pixel default_bg; + Pixel light; + Pixmap grey_stipple; + XawSheetHilight default_sh; +} SheetPart; + +typedef struct _SheetRec { + CorePart core; + SimplePart simple; + SheetPart sheet; +} SheetRec; + +#endif /* _SheetP_h */ diff --git a/src/staden/SmeLine.c b/src/staden/SmeLine.c new file mode 100644 index 0000000..70eea17 --- /dev/null +++ b/src/staden/SmeLine.c @@ -0,0 +1,301 @@ +#if ( !defined(lint) && !defined(SABER) ) +static char Xrcsid[] = "$XConsortium: SmeLine.c,v 1.7 89/12/11 15:20:17 kit Exp $"; +#endif + +/* + * Copyright 1989 Massachusetts Institute of Technology + * + * Permission to use, copy, modify, distribute, and sell this software and its + * documentation for any purpose is hereby granted without fee, provided that + * the above copyright notice appear in all copies and that both that + * copyright notice and this permission notice appear in supporting + * documentation, and that the name of M.I.T. not be used in advertising or + * publicity pertaining to distribution of the software without specific, + * written prior permission. M.I.T. makes no representations about the + * suitability of this software for any purpose. It is provided "as is" + * without express or implied warranty. + * + * M.I.T. DISCLAIMS ALL WARRANTIES WITH REGARD TO THIS SOFTWARE, INCLUDING ALL + * IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS, IN NO EVENT SHALL M.I.T. + * BE LIABLE FOR ANY SPECIAL, INDIRECT OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES + * WHATSOEVER RESULTING FROM LOSS OF USE, DATA OR PROFITS, WHETHER IN AN ACTION + * OF CONTRACT, NEGLIGENCE OR OTHER TORTIOUS ACTION, ARISING OUT OF OR IN + * CONNECTION WITH THE USE OR PERFORMANCE OF THIS SOFTWARE. + * + * Author: Chris D. Peterson, MIT X Consortium + */ + +/* + * Sme.c - Source code for the generic menu entry + * + * Date: September 26, 1989 + * + * By: Chris D. Peterson + * MIT X Consortium + * kit@expo.lcs.mit.edu + */ + +#include +#include +#include + +#include +#include +#include + +#define offset(field) XtOffset(SmeLineObject, sme_line.field) +static XtResource resources[] = { + {XtNlineWidth, XtCLineWidth, XtRDimension, (Cardinal)sizeof(Dimension), + offset(line_width), XtRImmediate, (XtPointer) 1}, + {XtNstipple, XtCStipple, XtRBitmap, (Cardinal)sizeof(Pixmap), + offset(stipple), XtRImmediate, (XtPointer) XtUnspecifiedPixmap}, + {XtNforeground, XtCForeground, XtRPixel, (Cardinal)sizeof(Pixel), + offset(foreground), XtRString, "XtDefaultForeground"}, +}; +#undef offset + +/* + * Function definitions. + */ + +static void Redisplay(), ClassInitialize(), Initialize(); +static void DestroyGC(), CreateGC(); +static Boolean SetValues(); +static XtGeometryResult QueryGeometry(); + + +#define SUPERCLASS (&smeClassRec) + +SmeLineClassRec smeLineClassRec = { + { + /* superclass */ (WidgetClass) SUPERCLASS, + /* class_name */ "SmeLine", + /* size */ (Cardinal)sizeof(SmeLineRec), + /* class_initialize */ ClassInitialize, + /* class_part_initialize*/ NULL, + /* Class init'ed */ FALSE, + /* initialize */ Initialize, + /* initialize_hook */ NULL, + /* realize */ NULL, + /* actions */ NULL, + /* num_actions */ ZERO, + /* resources */ resources, + /* resource_count */ XtNumber(resources), + /* xrm_class */ NULLQUARK, + /* compress_motion */ FALSE, + /* compress_exposure */ FALSE, + /* compress_enterleave*/ FALSE, + /* visible_interest */ FALSE, + /* destroy */ DestroyGC, + /* resize */ NULL, + /* expose */ Redisplay, + /* set_values */ SetValues, + /* set_values_hook */ NULL, + /* set_values_almost */ XtInheritSetValuesAlmost, + /* get_values_hook */ NULL, + /* accept_focus */ NULL, + /* intrinsics version */ XtVersion, + /* callback offsets */ NULL, + /* tm_table */ NULL, + /* query_geometry */ QueryGeometry, + /* display_accelerator*/ NULL, + /* extension */ NULL + },{ + /* Menu Entry Fields */ + + /* highlight */ XtInheritHighlight, + /* unhighlight */ XtInheritUnhighlight, + /* notify */ XtInheritNotify, + /* extension */ NULL + },{ + /* Line Menu Entry Fields */ + /* extension */ NULL + } +}; + +WidgetClass smeLineObjectClass = (WidgetClass) &smeLineClassRec; + +/************************************************************ + * + * Semi-Public Functions. + * + ************************************************************/ + +/* Function Name: ClassInitialize + * Description: The Line Menu Entry Object's class initialization proc. + * Arguments: none. + * Returns: none. + */ + +static void +ClassInitialize() +{ + static XtConvertArgRec screenConvertArg[] = { + {XtWidgetBaseOffset, (caddr_t) XtOffset(Widget, core.screen), + (Cardinal)sizeof(Screen *)} + }; + + XawInitializeWidgetSet(); + XtAddConverter(XtRString, XtRBitmap, XmuCvtStringToBitmap, + screenConvertArg, XtNumber(screenConvertArg)); +} + +/* Function Name: Initialize + * Description: Initializes the simple menu widget + * Arguments: request - the widget requested by the argument list. + * new - the new widget with both resource and non + * resource values. + * Returns: none. + */ + +/* ARGSUSED */ +static void +Initialize(request, new) +Widget request, new; +{ + SmeLineObject entry = (SmeLineObject) new; + + if (entry->rectangle.height == 0) + entry->rectangle.height = entry->sme_line.line_width; + + CreateGC(new); +} + +/* Function Name: CreateGC + * Description: Creates the GC for the line entry widget. + * Arguments: w - the Line entry widget. + * Returns: none + * + * We can only share the GC if there is no stipple, because + * we need to change the stipple origin when drawing. + */ + +static void +CreateGC(w) +Widget w; +{ + SmeLineObject entry = (SmeLineObject) w; + XGCValues values; + XtGCMask mask = GCForeground | GCGraphicsExposures | GCLineWidth ; + + values.foreground = entry->sme_line.foreground; + values.graphics_exposures = FALSE; + values.line_width = entry->sme_line.line_width; + + if (entry->sme_line.stipple != XtUnspecifiedPixmap) { + values.stipple = entry->sme_line.stipple; + values.fill_style = FillStippled; + mask |= GCStipple | GCFillStyle; + + entry->sme_line.gc = XCreateGC(XtDisplayOfObject(w), + RootWindowOfScreen(XtScreenOfObject(w)), + mask, &values); + } + else + entry->sme_line.gc = XtGetGC(w, mask, &values); +} + +/* Function Name: DestroyGC + * Description: Destroys the GC when we are done with it. + * Arguments: w - the Line entry widget. + * Returns: none + */ + +static void +DestroyGC(w) +Widget w; +{ + SmeLineObject entry = (SmeLineObject) w; + + if (entry->sme_line.stipple != XtUnspecifiedPixmap) + XFreeGC(XtDisplayOfObject(w), entry->sme_line.gc); + else + XtReleaseGC(w, entry->sme_line.gc); +} + +/* Function Name: Redisplay + * Description: Paints the Line + * Arguments: w - the menu entry. + * event, region - NOT USED. + * Returns: none + */ + +static void +Redisplay(w, event, region) +Widget w; +XEvent * event; +Region region; +{ + SmeLineObject entry = (SmeLineObject) w; + int y = entry->rectangle.y + + (entry->rectangle.height - entry->sme_line.line_width) / 2; + + if (entry->sme_line.stipple != XtUnspecifiedPixmap) + XSetTSOrigin(XtDisplayOfObject(w), entry->sme_line.gc, 0, y); + + XFillRectangle(XtDisplayOfObject(w), XtWindowOfObject(w), + entry->sme_line.gc, + 0, y, (unsigned int) entry->rectangle.width, + (unsigned int) entry->sme_line.line_width ); +} + +/* Function Name: SetValues + * Description: Relayout the menu when one of the resources is changed. + * Arguments: current - current state of the widget. + * request - what was requested. + * new - what the widget will become. + * Returns: none + */ + +/* ARGSUSED */ +static Boolean +SetValues(current, request, new) +Widget current, request, new; +{ + SmeLineObject entry = (SmeLineObject) new; + SmeLineObject old_entry = (SmeLineObject) current; + + if ( (entry->sme_line.line_width != old_entry->sme_line.line_width) && + (entry->sme_line.stipple != old_entry->sme_line.stipple) ) { + DestroyGC(current); + CreateGC(new); + return(TRUE); + } + return(FALSE); +} + +/* Function Name: QueryGeometry. + * Description: Returns the preferred geometry for this widget. + * Arguments: w - the menu entry object. + * itended, return - the intended and return geometry info. + * Returns: A Geometry Result. + * + * See the Intrinsics manual for details on what this function is for. + * + * I just return the height and a width of 1. + */ + +static XtGeometryResult +QueryGeometry(w, intended, return_val) +Widget w; +XtWidgetGeometry *intended, *return_val; +{ + SmeObject entry = (SmeObject) w; + Dimension width; + XtGeometryResult ret_val = XtGeometryYes; + XtGeometryMask mode = intended->request_mode; + + width = 1; /* we can be really small. */ + + if ( ((mode & CWWidth) && (intended->width != width)) || + !(mode & CWWidth) ) { + return_val->request_mode |= CWWidth; + return_val->width = width; + mode = return_val->request_mode; + + if ( (mode & CWWidth) && (width == entry->rectangle.width) ) + return(XtGeometryNo); + return(XtGeometryAlmost); + } + return(ret_val); +} diff --git a/src/staden/aix-bits.c b/src/staden/aix-bits.c new file mode 100644 index 0000000..fab2e8f --- /dev/null +++ b/src/staden/aix-bits.c @@ -0,0 +1,15 @@ +#include + +/* + * KH 2/06/92 + * simulate a CALL FLUSH(devsepc) from SUN FORTRAN + * for STADEN programs + */ + +void * flush (int *fd) +{ + FILE *a = fdopen(*fd,"a"); + if (ferror(a) ) perror("problems with flushing: fdopen(int, char *)"); + fflush(a); + if (ferror(a) ) perror("problems with flushing: fflush(FILE *)"); +} diff --git a/src/staden/analps89.f b/src/staden/analps89.f new file mode 100644 index 0000000..3c4caba --- /dev/null +++ b/src/staden/analps89.f @@ -0,0 +1,2329 @@ +C SUBROUTINES FOR ANALYSEP +C AUTHOR: RODGER STADEN +C 8-2-90 back translation: zeroed righthand end of workr +C 9-7-90 removed menu routine +C 24-7-90 edited helixw to use getint for compatibility with x versions +C 5-11-90 signlp call to rdwmt added zero as last parameter +C 4-12-90 Changed name of compc to compcp for the stupid sun linker +C 21-12-90 Changed signlp so that text output gives position as +C I+MIDDLE (was I) + SUBROUTINE SETPAR(IOK) + PARAMETER (MINOPT = 17, + + MAXOPT = 28, + + NUMBER = 9) + INTEGER VALUES(NUMBER,MINOPT:MAXOPT) +C INTEGER TEMP(NUMBER) + SAVE /PAMDEF/ + COMMON /PAMDEF/VALUES + IOK = 0 +C SEARCH +C WRITE(KBOUT,*)' Search for short sequences' +C CALL GETPAR(17,7,IOK,IFILE,OPTION,OPTOUT,NOPOUT, +C +MININC,MAXINC,INC) + VALUES(1,17) = 1 + VALUES(2,17) = 0 + VALUES(3,17) = 0 + VALUES(4,17) = 0 + VALUES(5,17) = 1 + VALUES(6,17) = 12 + VALUES(7,17) = 1 +C PERCENT MATCH + VALUES(1,18) = 0 + VALUES(2,18) = 70 +C SCORE MATRIX MATCH + VALUES(1,19) = 0 +C WEIGHT MATRIX +C WRITE(KBOUT,*)' MOTIF SEARCH USING WEIGHT MATRIX' +C CALL GETPAR(20,2,IOK,IOPT,IGON) + VALUES(1,20) = 0 + VALUES(2,20) = 0 +C HYDROPHOBICITY + VALUES(1,22) = 1 + VALUES(2,22) = 101 + VALUES(3,22) = 11 + VALUES(4,22) = 1 + VALUES(5,22) = 101 + VALUES(6,22) = 3 +C CHARGE + VALUES(1,23) = 1 + VALUES(2,23) = 101 + VALUES(3,23) = 11 + VALUES(4,23) = 1 + VALUES(5,23) = 101 + VALUES(6,23) = 3 +C ROBSON +C CALL GETPAR(24,4,IOK,MINIW,MAXIW,IWRIT,IGON) + VALUES(1,24) = 1 + VALUES(2,24) = 101 + VALUES(3,24) = 1 + VALUES(4,24) = 0 +C HYDROPHOBIC MOMENT + VALUES(1,25) = 1 + VALUES(2,25) = 130 + VALUES(3,25) = 100 + VALUES(4,25) = 1 + VALUES(5,25) = 60 + VALUES(6,25) = 18 + VALUES(7,25) = 1 + VALUES(8,25) = 101 + VALUES(9,25) = 3 +C HELIX WHEEL + VALUES(1,26) = 1 + VALUES(2,26) = 130 + VALUES(3,26) = 100 + VALUES(4,26) = 1 + VALUES(5,26) = 60 + VALUES(6,26) = 18 +C BACK TRANSLATE + VALUES(1,27) = 0 + VALUES(2,27) = 0 + VALUES(3,27) = 1 + VALUES(4,27) = 11 + VALUES(5,27) = 11 + VALUES(6,27) = 1 + VALUES(7,27) = 11 + VALUES(8,27) = 3 +C PATTERNS TO DO +C OPEN(UNIT=99,FILE='ANALYSEP.PAR',STATUS ='OLD') +C10 CONTINUE +C READ(99,*,ERR=100,END=200)TEMP +C IF((TEMP(1).GE.MINOPT).AND.(TEMP(1).LE.MAXOPT)) THEN +C DO 20 I = 1,NUMBER-1 +C VALUES(I,TEMP(1)) = TEMP(I+1) +C WRITE(*,*)TEMP(1),TEMP(I+1) +C20 CONTINUE +C END IF +C GO TO 10 +C100 CONTINUE +C WRITE(*,*)'ERROR IN PARMS FILE' +C GO TO 10 +C200 CONTINUE +C CLOSE(UNIT = 99) + IOK = 0 + END + SUBROUTINE GETPAR(IOPT,NPAM,IOK,I1,I2,I3,I4,I5,I6,I7,I8,I9) + PARAMETER (MINOPT = 17, + + MAXOPT = 28, + + NUMBER = 9) + INTEGER VALUES(NUMBER,MINOPT:MAXOPT) + COMMON /PAMDEF/VALUES + IOK = 0 + IF((IOPT.GE.MINOPT).AND.(IOPT.LE.MAXOPT)) THEN + I = 1 + I1 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I2 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I3 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I4 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I5 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I6 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I7 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I8 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I9 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + END IF + IOK = 1 + END +C COMPH + SUBROUTINE COMPH(SEQ,IDIM1P,MXSPAN, + 1MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KBIN,KBOUT, + +SCORES,IDSCOR, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + CHARACTER SEQ(IDIM1P) + INTEGER DIALOG + INTEGER SPAN + REAL SCORES(IDSCOR) + CALL SHOWFU(KBOUT,'Plot hydrophobicity') + CALL GETPAR(22,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9) + IF(IOK.NE.0) RETURN + IF(DIALOG.EQ.1) THEN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL PHYDRO(SEQ,IDIM1P,MXSPAN, + 1MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2, + +SCORES,IDSCOR,SPAN,IWRIT) + END + SUBROUTINE PHYDRO(SEQ,IDIM1P,MXSPAN, + 1MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2, + +SCORES,IDSCOR,SPAN,IWRIT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,CTONUM + REAL SCORES(IDSCOR) + EXTERNAL CTONUM +C max score + SCRMAX=-9999. + SCRMIN=99999. + DO 1 I=1,IDSCOR + IF(SCORES(I).GT.SCRMAX)SCRMAX=SCORES(I) + IF(SCORES(I).LT.SCRMIN)SCRMIN=SCORES(I) +1 CONTINUE + I1INM1=1+MXSPAN/2 + J1P=J1+I1INM1 + J2P=J2+I1INM1 + XMIN=J1 + XMAX=J2 +C length forward and back + LF=SPAN/2 + LB=1+SPAN/2 +C set ymax + RANGE=SPAN*(SCRMAX-SCRMIN) + RANGEP=RANGE/100. + YMIN=SPAN*SCRMIN + YMAX=SPAN*SCRMAX + XBIT=XMIN+(XMAX-XMIN)*0.01 + YMID=YMIN+RANGE*0.5 + YMID45=YMID-RANGEP*5.0 +C do edge + CALL STARTR(SEQ(J1P-LB),IDIM1P,SCORES,IDSCOR,SPAN,SUM) + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + DO 40 K=-40,40,10 + YPLOT=YMID+RANGEP*K + CALL LINE(XMIN,XBIT,YPLOT,YPLOT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +40 CONTINUE +C PLOT MID LINE + CALL LINE(XMIN,XMAX,YMID45,YMID45,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C do rest + IDONE=0 + XF=J1 + YF=SUM + DO 100 I=J1P,J2P + IDONE=IDONE+1 + SUM=SUM+SCORES(CTONUM(SEQ(I+LF))) + + -SCORES(CTONUM(SEQ(I-LB))) + IF(MOD(I,IWRIT).EQ.0)THEN + XT=I-I1INM1 + CALL LINE(XF,XT,YF,SUM,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XF=XT + YF=SUM + END IF +100 CONTINUE + CALL VT100M + END +C COMPCP + SUBROUTINE COMPCP(SEQ,IDIM1P,MXSPAN, + 1MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KBIN,KBOUT, + +SCORES,IDSCOR, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + CHARACTER SEQ(IDIM1P) + INTEGER DIALOG + INTEGER SPAN + REAL SCORES(IDSCOR) + CALL SHOWFU(KBOUT,'Plot charge') + CALL GETPAR(23,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9) + IF(IOK.NE.0) RETURN + IF(DIALOG.EQ.1) THEN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL PCOMPC(SEQ,IDIM1P,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2, + +SCORES,IDSCOR,SPAN,IWRIT) + END + SUBROUTINE PCOMPC(SEQ,IDIM1P,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2, + +SCORES,IDSCOR,SPAN,IWRIT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,CTONUM + REAL SCORES(IDSCOR) + EXTERNAL CTONUM + SCRMAX=-9999. + SCRMIN=9999. + DO 1 I=1,IDSCOR + IF(SCORES(I).GT.SCRMAX)SCRMAX=SCORES(I) + IF(SCORES(I).LT.SCRMIN)SCRMIN=SCORES(I) +1 CONTINUE +C length forward and back + LF=SPAN/2 + LB=1+SPAN/2 + I1INM1=1+MXSPAN/2 + J1P=J1+I1INM1 + J2P=J2+I1INM1 + XMIN=J1 + XMAX=J2 +C set ymax + RANGE=SPAN*(SCRMAX-SCRMIN) + RANGEP=RANGE/100. + YMIN=SPAN*SCRMIN + YMAX=SPAN*SCRMAX + XBIT=XMIN+(XMAX-XMIN)*0.01 + YMID=YMIN+RANGE*0.5 +C do edge + CALL STARTR(SEQ(J1P-LB),IDIM1P,SCORES,IDSCOR,SPAN,SUM) + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + DO 40 K=-40,40,10 + YPLOT=YMID+RANGEP*K + CALL LINE(XMIN,XBIT,YPLOT,YPLOT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +40 CONTINUE +C PLOT MID LINE + CALL LINE(XMIN,XMAX,YMID,YMID,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C do rest + IDONE=0 + XF=J1 + YF=SUM + DO 100 I=J1P,J2P + IDONE=IDONE+1 + SUM=SUM+SCORES(CTONUM(SEQ(I+LF))) + + -SCORES(CTONUM(SEQ(I-LB))) + IF(MOD(I,IWRIT).EQ.0)THEN + XT=I-I1INM1 + CALL LINE(XF,XT,YF,SUM,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XF=XT + YF=SUM + END IF +100 CONTINUE + CALL VT100M + END + SUBROUTINE STARTR(SEQ,IDIM,SCORES,IDIMS,LENW,SUM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + REAL SCORES(IDIMS) + INTEGER CTONUM + EXTERNAL CTONUM + SUM=0. + DO 10 I=1,LENW + SUM = SUM + SCORES(CTONUM(SEQ(I))) +10 CONTINUE + END +C this routine uses the following arrays and variables: +C a concatenated array of recognition sequences recseq maxrec idrseq prec +C a concatenated array of enzyme names names maxnam idnaml pnam +C a list of pointers to enzyme names in names namep maxnam itoten +C a list of enzyme name lengths namlen maxen itoten +C a list of numbers of recognition sequences per enzyme numrec maxen +C a list of lenghts of recognition sequences per enzyme (all expected +C to be the same) lenen maxen itoten +C a list of pointers to the first character of the first recognition +C sequence per enzyme recstr maxen itoten +C it also needs temporary storage of matches in psave and nsave of +C size maxmat and temporary storage of lengths of fragments in length +C output of results is on unit idev + SUBROUTINE SERCHP(SEQ,IDSEQ,J1,J2,KSTART, + +RENZYM,MAXREN,RECSEQ,MAXREC, + +NAMES,MAXNAM,LENGTH,PSAVE,NSAVE,MAXMAT, + +NAMEP,NAMLEN,NUMREC,LENEN,RECSTR,NAMLST,MAXENZ, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV, + +IDEVEN,FILNAM,FILEA,FILEAA,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),FILEA*(*),FILEAA*(*),HELPF*(*) + CHARACTER SEQ(IDSEQ),RENZYM(MAXREN),RECSEQ(MAXREC),NAMES(MAXNAM) + INTEGER OPTION,OPTOUT + INTEGER LENGTH(MAXMAT),PSAVE(MAXMAT),NSAVE(MAXMAT) + INTEGER NAMEP(MAXENZ),NAMLEN(MAXENZ),NUMREC(MAXENZ) + INTEGER LENEN(MAXENZ) + INTEGER RECSTR(MAXENZ),NAMLST(MAXENZ),DIALOG + PARAMETER (IBH=256) + CALL SHOWFU(KBOUT,'Search for short sequences') + CALL GETPAR(17,7,IOK,IFILE,OPTION,OPTOUT,NOPOUT, + +MININC,MAXINC,INC, + +IPAR8,IPAR9) + IF(IOK.NE.0) RETURN +10 CONTINUE + IDREN = MAXREN + IDRSEQ = MAXREC + IDNAML = MAXNAM + IDNLST = MAXENZ + MAXEN = MAXENZ + IDMAT = MAXMAT + LEVEL = 0 + CALL SERCHD(RENZYM,IDREN,RECSEQ,IDRSEQ, + +NAMES,IDNAML,MARGT, + +NAMEP,NAMLEN,NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST, + +IDEVEN,FILNAM,FILEA,FILEAA,KBIN,KBOUT, + +OPTION,OPTOUT,NOPOUT,IFILE,MININC,MAXINC,INC,DIALOG, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL BUSY(KBOUT) + IF(OPTOUT.EQ.2) THEN +C CALL FINDL1(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ,NAMES,IDNAML, +C + NAMEP,NAMLEN, +C + NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, +C + PSAVE,NSAVE,LENGTH,IDMAT,NOPOUT,IBH,LEVEL, +C + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, +C + IHELPS,IHELPE,HELPF,IDEVH) + ELSE IF((OPTOUT.EQ.0).AND.(NOPOUT.EQ.1)) THEN + CALL FINDL2(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ,NAMES,IDNAML, + + NAMEP,NAMLEN, + + NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PSAVE,NSAVE,LENGTH,IDMAT,NOPOUT,IBH,LEVEL, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + ELSE IF((OPTOUT.EQ.1).AND.(NOPOUT.EQ.1)) THEN + CALL FINDL3(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ,NAMES,IDNAML, + + NAMEP,NAMLEN, + + NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PSAVE,NSAVE,LENGTH,IDMAT,NOPOUT,IBH,LEVEL, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + ELSE IF((OPTOUT.EQ.0).AND.(NOPOUT.EQ.0)) THEN + CALL FINDL4(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ,NAMES,IDNAML, + + NAMEP,NAMLEN, + + NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PSAVE,NSAVE,LENGTH,IDMAT,NOPOUT,IBH,LEVEL, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + ELSE IF((OPTOUT.EQ.1).AND.(NOPOUT.EQ.0)) THEN + CALL FINDL5(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ,NAMES,IDNAML, + + NAMEP,NAMLEN, + + NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PSAVE,NSAVE,LENGTH,IDMAT,NOPOUT,IBH,LEVEL, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + END IF + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + DIALOG = 1 + GO TO 10 + END + SUBROUTINE GETRNP(RENZYM,IDREN,IDEV,FILNAM,FILEA,FILEAA, + + IOK,KBIN,KBOUT,IFILE,DIALOG, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),FILEA*(*),FILEAA*(*),RENZYM(IDREN) + INTEGER DIALOG + PARAMETER (MAXPRM = 16) + CHARACTER PROMPT(4)*(MAXPRM) + IOK = 1 + IF(DIALOG.EQ.0) NOPT = IFILE + IOP = 1 +1 CONTINUE + IF(DIALOG.EQ.1) THEN + PROMPT(1) = 'Search' + PROMPT(2) = 'List enzyme file' + PROMPT(3) = 'Clear text' + PROMPT(4) = 'Clear graphics' + IOP = 1 + CALL RADION('Select option',PROMPT,4,IOP, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.1) THEN + IOK = 1 + RETURN + END IF + IF(IOP.EQ.3) THEN + CALL CLEARV + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + GO TO 1 + END IF + IF(IOP.EQ.4) THEN + CALL CLEARG + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + GO TO 1 + END IF + IF((IOP.EQ.1).OR.(IOP.EQ.2)) THEN + NOPT = IFILE + PROMPT(1) = 'All acids' + PROMPT(2) = 'Named groups' + PROMPT(3) = 'Personal file' + PROMPT(4) = 'Keyboard' + CALL RADION('Select string definition mode',PROMPT,4,NOPT, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(NOPT.LT.1) THEN + IOK = 1 + RETURN + END IF + END IF + END IF + IF(NOPT.EQ.1)THEN + CALL OPENRS(IDEV,FILEAA,IOK,LRECL,2) + IF(IOK.NE.0)GO TO 100 + IF(IOP.EQ.2)THEN + CALL TTEXT1(IDEV,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IQUIT) + GO TO 1 + END IF + CALL ARRFIL(IDEV,RENZYM,IDREN,KBOUT) + CLOSE(UNIT=IDEV) + RETURN + END IF + IF(NOPT.EQ.2)THEN + CALL OPENRS(IDEV,FILEA,IOK,LRECL,2) + IF(IOK.NE.0)GO TO 100 + IF(IOP.EQ.2)THEN + CALL TTEXT1(IDEV,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IQUIT) + GO TO 1 + END IF + CALL ARRFIL(IDEV,RENZYM,IDREN,KBOUT) + CLOSE(UNIT=IDEV) + RETURN + END IF + IF(NOPT.EQ.3)THEN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + + 'Named sequences file', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.EQ.2) GO TO 1 + IF(IOK.NE.0)GO TO 100 + IF(IOP.EQ.2)THEN + CALL TTEXT1(IDEV,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IQUIT) + GO TO 1 + END IF + CALL ARRFIL(IDEV,RENZYM,IDREN,KBOUT) + CLOSE(UNIT=IDEV) + RETURN + END IF + IF(NOPT.EQ.4)THEN + CALL GETRKB(RENZYM,IDREN,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH, + + IOK) + RETURN + END IF + GO TO 1 +100 CONTINUE + CALL ERROM(KBOUT,'Error opening file') + IOK=1 + GO TO 1 + END + SUBROUTINE DECREN(RENZYM,IDREN,RECSEQ,IDRSEQ,NAMES,IDNAML, + +NAMEP,NAMLEN,NUMREC,LENEN,RECSTR,MAXEN,KBOUT) +C routine to decode a restriction enzyme file array renzym idren +C into the following: +C a concatenated array of recognition sequences recseq idrseq prec +C a concatenated array of enzyme names names idnaml,pnam +C a list of pointers to enzyme names in names namep maxen itoten +C a list of enzyme name lengths namlen maxen itoten +C a list of numbers of recognition sequences per enzyme numrec maxen +C a list of lenghts of recognition sequences per enzyme (all expected +C to be the same) lenen maxen itoten +C a list of pointers to the first character of the first recognition +C sequence per enzyme recstr maxen itoten +C AUTHOR: RODGER STADEN + CHARACTER RENZYM(IDREN),RECSEQ(IDRSEQ),NAMES(IDNAML) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN),LENEN(MAXEN) + INTEGER RECSTR(MAXEN),TOTEN,PREC,PREN,PNAM + TOTEN=0 + PREC=1 + PREN=0 + PNAM=1 +10 CONTINUE + PREN=PREN+1 + IF(PREN.LT.IDREN)THEN + CALL CHARCT(RENZYM,IDREN,PREN,NCHAR) + IF(NCHAR.GT.0)THEN + TOTEN=TOTEN+1 + IF(TOTEN.LT.MAXEN)THEN + IF((PNAM+NCHAR-1).GT.IDNAML)THEN + WRITE(KBOUT,1006) +1006 FORMAT(' Names array full') + GO TO 101 + END IF + NAMEP(TOTEN)=PNAM + NAMLEN(TOTEN)=NCHAR + CALL SQCOPY(RENZYM(PREN),NAMES(PNAM),NCHAR) + PNAM=PNAM+NCHAR + NREC=0 + LMAX=0 +20 CONTINUE + PREN=PREN+NCHAR+1 + NCHAR=0 + IF(PREN.LT.IDREN)CALL CHARCT(RENZYM,IDREN,PREN,NCHAR) + IF(NCHAR.EQ.0)THEN + IF(NREC.EQ.0)THEN + WRITE(KBOUT,1002) +1002 FORMAT(' Name with no strings!') + TOTEN=TOTEN-1 + GO TO 100 + END IF + NUMREC(TOTEN)=NREC + LENEN(TOTEN)=LMAX + GO TO 10 + END IF + NREC=NREC+1 + IF(NCHAR.GT.LMAX)LMAX=NCHAR + CALL SQCOPY(RENZYM(PREN),RECSEQ(PREC),NCHAR) + IF(NREC.EQ.1)RECSTR(TOTEN)=PREC + PREC=PREC+NCHAR + GO TO 20 + END IF + CALL ERROM(KBOUT,'Too many names') + GO TO 100 + END IF + CALL ERROM(KBOUT,'Error in names and strings file') + END IF +100 CONTINUE +101 CONTINUE +C WRITE(KBOUT,1001)TOTEN +C1001 FORMAT(' number of names=',I6) + IDNAML=PNAM-1 + MAXEN=TOTEN + IDRSEQ=PREC + END + SUBROUTINE FIND4(SEQ,IDIM1,STRING,IDIM2,INC,IMATCH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2),DASH + INTEGER PSEQ,PSTR + SAVE DASH + DATA DASH/'-'/ + PSEQ=1-1*INC + PSTR=1 + IMATCH=0 +100 CONTINUE + PSEQ=PSEQ+1-PSTR + PSEQ=PSEQ+INC-1 +400 CONTINUE + PSTR=0 +500 CONTINUE + PSEQ=PSEQ+1 + IF(PSEQ.GT.IDIM1)GO TO 300 + PSTR=PSTR+1 + IF(STRING(PSTR).EQ.DASH)GO TO 450 + IF(SEQ(PSEQ).NE.STRING(PSTR))GO TO 100 +450 CONTINUE + IF(PSTR.LT.IDIM2)GO TO 500 + IMATCH=PSEQ-IDIM2+1 +300 CONTINUE + END + SUBROUTINE S1(SEQ,IDSEQ,NREC,LREC,JEN,IREC,J1,J2,INC,KSTART, + +RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND,KBOUT,IOK) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),RECSEQ(IDRSEQ) + INTEGER RECSTR(MAXEN),PSAVE(MAXMAT),NSAVE(MAXMAT) + IOK = 1 + DO 50 IREC=1,NREC + ISTR=RECSTR(JEN)+(IREC-1)*(LREC) +C NOTE WE NEED TO POINT TO THE CORRECT ARRAY ELEMENT BUT +C TO REMEMBER THE SEQUENCE ELEMENT FOR OUTPUT +C IAT IS THE ARRAY ELEMENT + IAT=J1-INC-KSTART+1 +10 CONTINUE + IAT=IAT+INC + ILEFT=J2-IAT+1-KSTART+1 + IF(ILEFT.GT.0)THEN + CALL FIND8(SEQ(IAT),ILEFT, + + RECSEQ(ISTR),LREC,INC,IMATCH) + IF(IMATCH.GT.0)THEN + IFOUND=IFOUND+1 + IF(IFOUND.GT.MAXMAT)THEN + CALL ERROM(KBOUT,'Too many matches') + RETURN + END IF + IAT=IAT+IMATCH-1 +C SAVE POSITION RELATIVE TO ARRAY + PSAVE(IFOUND)=IAT + NSAVE(IFOUND)=JEN + GO TO 10 + END IF + END IF +50 CONTINUE + IOK = 0 + END + SUBROUTINE S2(SEQ,IDSEQ,IFOUND,NAMEP,NAMLEN,LENEN,MAXEN, + +NSAVE,PSAVE,LENGTH,MAXMAT,NAMES,IDNAML, + +LENN,IDEV,J2,KSTART,JOB) + PARAMETER (IDT = 20) + CHARACTER SEQ(IDSEQ),TEMP1(IDT),TEMP2(IDT),NAMES(IDNAML) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),LENEN(MAXEN) + INTEGER NSAVE(MAXMAT),PSAVE(MAXMAT),LENGTH(MAXMAT) + DO 60 KOUT=1,IFOUND + KR1=NAMEP(NSAVE(KOUT)) + KS1=PSAVE(KOUT) + CALL FILLC(TEMP1,IDT,' ') + CALL FILLC(TEMP2,IDT,' ') + KR2 = NAMLEN(NSAVE(KOUT)) + CALL SQCOPY(NAMES(KR1),TEMP1,KR2) + KR2 = LENEN(NSAVE(KOUT)) + CALL SQCOPY(SEQ(KS1),TEMP2,KR2) + LENN=PSAVE(KOUT)-LENN + IF(JOB.EQ.0) THEN + WRITE(IDEV,1008) + + TEMP1,TEMP2,PSAVE(KOUT)+KSTART-1,LENN,LENGTH(KOUT) + ELSE IF(JOB.EQ.1) THEN + WRITE(IDEV,1008) + + TEMP1,TEMP2,PSAVE(KOUT)+KSTART-1,LENGTH(KOUT) + END IF + LENN=PSAVE(KOUT) +1008 FORMAT(' ',20A1,2X,20A1,2X,I6,2X,I6,2X,I6) +60 CONTINUE + IF(JOB.EQ.0) THEN + LEND=J2-PSAVE(IFOUND)+1-KSTART+1 + WRITE(IDEV,1020)LEND,LENGTH(IFOUND+1) +1020 FORMAT(' ',52X,I6,2X,I6) + ELSE IF (JOB.EQ.1) THEN + LEND=J2-PSAVE(IFOUND)+1-KSTART+1 + WRITE(IDEV,1020)LEND + END IF + END + SUBROUTINE FINDL2(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ, + 1NAMES,IDNAML,NAMEP,NAMLEN, + 1NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + 1PSAVE,NSAVE,LENGTH,MAXMAT,NOPOUT,IBH,LEVEL, + 1MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),NAMES(IDNAML),RECSEQ(IDRSEQ) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN),LENEN(MAXEN) + INTEGER RECSTR(MAXEN),NAMLST(IDNLST),PSAVE(MAXMAT) + INTEGER LENGTH(MAXMAT) + INTEGER NSAVE(MAXMAT),OPTION,OPTOUT + CHARACTER HELPF*(*) + CHARACTER*20 ATOS + EXTERNAL ATOS +C OPTOUT=0 ENZYME BY ENZYME +C NOPOUT=1 GRAPHICAL OUTPUT +C FOR GRAPHICS NEED TO CHECK FOR END OF SCREEN + CALL CLEARV + CALL VECTOM + LEVEL1=LEVEL + LEVELM=MARGT-IBH +C names max length 20 chars, strings 20 chars + LAST=MAXEN + IF(OPTION.EQ.1)LAST=IDNLST + DO 100 IEN=1,LAST + IFOUND=0 + JEN=IEN + IF(OPTION.EQ.1)JEN=NAMLST(IEN) + NREC=NUMREC(JEN) + LREC=LENEN(JEN) + CALL S1(SEQ,IDSEQ,NREC,LREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND,KBOUT, + + IOK) + IF(IOK.NE.0) THEN + CALL VT100M + RETURN + END IF + IF(IFOUND.GT.0) THEN + IF(LEVEL.GE.LEVELM)THEN + CALL PSRCHX(LEVEL1,LEVEL,KBIN,KBOUT,IQUIT, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IQUIT.EQ.1) THEN + CALL VT100M + RETURN + END IF + END IF + CALL PLSRCH(J1-KSTART+1,J2-KSTART+1, + + PSAVE,IFOUND,IBH,LEVEL, + + ATOS(NAMES(NAMEP(JEN)),NAMLEN(JEN)), + + NAMLEN(JEN), + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF +100 CONTINUE + CALL VT100M + END + SUBROUTINE FINDL3(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ, + +NAMES,IDNAML,NAMEP,NAMLEN, + +NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + +PSAVE,NSAVE,LENGTH,MAXMAT,NOPOUT,IBH,LEVEL, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),NAMES(IDNAML),RECSEQ(IDRSEQ) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN),LENEN(MAXEN) + INTEGER RECSTR(MAXEN),NAMLST(IDNLST),PSAVE(MAXMAT) + INTEGER LENGTH(MAXMAT) + INTEGER NSAVE(MAXMAT),OPTION,OPTOUT + CHARACTER HELPF*(*) +C OPTOUT=1 ALL ENZYMES TOGETHER +C NOPOUT=1 GRAPHICAL OUTPUT +C FOR GRAPHICS NEED TO CHECK FOR END OF SCREEN + CALL CLEARV + CALL VECTOM + LEVEL1=LEVEL + LEVELM=MARGT-IBH +C names max length 20 chars, strings 20 chars + LAST=MAXEN + IF(OPTION.EQ.1)LAST=IDNLST + IFOUND=0 + DO 100 IEN=1,LAST + JEN=IEN + IF(OPTION.EQ.1)JEN=NAMLST(IEN) + NREC=NUMREC(JEN) + LREC=LENEN(JEN) + CALL S1(SEQ,IDSEQ,NREC,LREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND,KBOUT, + + IOK) + IF(IOK.NE.0) THEN + CALL VT100M + RETURN + END IF +100 CONTINUE + IF(IFOUND.GT.0)THEN + CALL PLSRCH(J1-KSTART+1,J2-KSTART+1, + + PSAVE,IFOUND,IBH,LEVEL,'ALL',3, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + CALL VT100M + IF(IFOUND.EQ.0)WRITE(IDEV,3000) +3000 FORMAT(' no matches') + END + SUBROUTINE FINDL4(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ, + +NAMES,IDNAML,NAMEP,NAMLEN, + +NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + +PSAVE,NSAVE,LENGTH,MAXMAT,NOPOUT,IBH,LEVEL, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),NAMES(IDNAML),RECSEQ(IDRSEQ) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN),LENEN(MAXEN) + INTEGER RECSTR(MAXEN),NAMLST(IDNLST),PSAVE(MAXMAT) + INTEGER LENGTH(MAXMAT) + INTEGER NSAVE(MAXMAT),OPTION,OPTOUT + CHARACTER HELPF*(*) +C OPTOUT=0 ENZYME BY ENZYME +C names max length 20 chars, strings 20 chars + LAST=MAXEN + IF(OPTION.EQ.1)LAST=IDNLST + DO 100 IEN=1,LAST + IFOUND=0 + JEN=IEN + IF(OPTION.EQ.1)JEN=NAMLST(IEN) + NREC=NUMREC(JEN) + LREC=LENEN(JEN) + CALL S1(SEQ,IDSEQ,NREC,LREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND,KBOUT, + + IOK) + IF(IOK.NE.0) RETURN + IF(IFOUND.GT.0) THEN + WRITE(IDEV,1010)IFOUND +1010 FORMAT(' matches=',I6) + CALL BUB2AS(PSAVE,NSAVE,IFOUND) +C calc lengths + LENGTH(1)=PSAVE(1)-(J1-KSTART+1) + DO 45 KOUT=2,IFOUND + LENGTH(KOUT)=PSAVE(KOUT)-PSAVE(KOUT-1) +45 CONTINUE +C do length to end + LENGTH(IFOUND+1)=J2-KSTART+1-PSAVE(IFOUND)+1 + IFP1=IFOUND+1 + CALL BUBBLE(LENGTH,IFP1) + LENN=J1-KSTART + WRITE(IDEV,1011) +1011 FORMAT(' NAME SEQUENCE POSITION', + + ' FRAGMENT LENGTHS') + CALL S2(SEQ,IDSEQ,IFOUND,NAMEP,NAMLEN,LENEN,MAXEN, + + NSAVE,PSAVE,LENGTH,MAXMAT,NAMES,IDNAML, + + LENN,IDEV,J2,KSTART,0) + END IF +100 CONTINUE + END + SUBROUTINE FINDL5(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ, + +NAMES,IDNAML,NAMEP,NAMLEN, + +NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + +PSAVE,NSAVE,LENGTH,MAXMAT,NOPOUT,IBH,LEVEL, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),NAMES(IDNAML),RECSEQ(IDRSEQ) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN),LENEN(MAXEN) + INTEGER RECSTR(MAXEN),NAMLST(IDNLST),PSAVE(MAXMAT) + INTEGER LENGTH(MAXMAT) + INTEGER NSAVE(MAXMAT),OPTION,OPTOUT + CHARACTER HELPF*(*) +C OPTOUT=1 ALL ENZYMES TOGETHER +C NOPOUT = 0 (LISTED) +C names max length 20 chars, strings 20 chars + LAST=MAXEN + IF(OPTION.EQ.1)LAST=IDNLST + IFOUND=0 + DO 100 IEN=1,LAST + JEN=IEN + IF(OPTION.EQ.1)JEN=NAMLST(IEN) + NREC=NUMREC(JEN) + LREC=LENEN(JEN) + CALL S1(SEQ,IDSEQ,NREC,LREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND,KBOUT, + + IOK) + IF(IOK.NE.0) RETURN +100 CONTINUE + IF(IFOUND.GT.0) THEN + CALL BUB2AS(PSAVE,NSAVE,IFOUND) + LENN=J1-KSTART + WRITE(IDEV,1011) +1011 FORMAT(' NAME SEQUENCE POSITION', + + ' FRAGMENT LENGTHS') + LENGTH(1)=PSAVE(1)-(J1-KSTART+1) + DO 59 KOUT=2,IFOUND + LENGTH(KOUT)=PSAVE(KOUT)-PSAVE(KOUT-1) +59 CONTINUE + CALL S2(SEQ,IDSEQ,IFOUND,NAMEP,NAMLEN,LENEN,MAXEN, + + NSAVE,PSAVE,LENGTH,MAXMAT,NAMES,IDNAML, + + LENN,IDEV,J2,KSTART,1) + ELSE + WRITE(IDEV,3000) +3000 FORMAT(' no matches') + END IF + END + SUBROUTINE SERCHD(RENZYM,IDREN,RECSEQ,IDRSEQ, + +NAMES,IDNAML,MARGT, + +NAMEP,NAMLEN,NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST, + +IDEVEN,FILNAM,FILEA,FILEAA,KBIN,KBOUT, + +OPTION,OPTOUT,NOPOUT,IFILE,MININC,MAXINC,INC,DIALOG, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),FILEA*(*),FILEAA*(*),HELPF*(*) + CHARACTER RENZYM(IDREN),RECSEQ(IDRSEQ),NAMES(IDNAML) + INTEGER OPTION,OPTOUT + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN) + INTEGER LENEN(MAXEN) + INTEGER RECSTR(MAXEN),NAMLST(IDNLST),DIALOG + IOK = 0 + CALL GETRNP(RENZYM,IDREN,IDEVEN,FILNAM,FILEA,FILEAA, + +IOK,KBIN,KBOUT,IFILE,DIALOG, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + IF(IDREN.LT.5) THEN + IOK = 1 + RETURN + END IF + CALL DECREN(RENZYM,IDREN,RECSEQ,IDRSEQ,NAMES,IDNAML, + +NAMEP,NAMLEN,NUMREC,LENEN,RECSTR,MAXEN,KBOUT) + IF(MAXEN.LT.1) THEN + IOK = 1 + RETURN + END IF + IF(DIALOG.EQ.0) RETURN + IOP = OPTION + CALL YESONO(IOP,'All names','Selected names', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) THEN + IOK = 1 + RETURN + END IF + OPTION = IOP + IF(OPTION.EQ.1)THEN + CALL GETNAM(NAMLST,IDNLST,NAMES,IDNAML,NAMEP,NAMLEN, + + MAXEN,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IDNLST.LT.1) THEN + IOK = 1 + RETURN + END IF + END IF + IOP = OPTOUT + CALL YESONO(IOP,'Show results name by name', + +'Show results all names together', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) THEN + IOK = 1 + RETURN + END IF + OPTOUT = IOP + IOP = NOPOUT + CALL YESONO(IOP,'List matches','Plot matches', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) THEN + IOK = 1 + RETURN + END IF + NOPOUT = IOP +C IF(NOPOUT.EQ.1)THEN +C LEVEL = LEVELI +C CALL SD2(IBLIPH,IBH,LEVEL,MARGT, +C + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) +C LEVELI = LEVEL +C IF(IOK.NE.0) RETURN +C END IF +C CALL GETINT(MININC,MAXINC,INC,'Step', +C +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) +C IF(IOK.NE.0) RETURN +C INC = IVAL + INC = 1 + END + SUBROUTINE SIGNLP(SEQ,IDIM,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,IDEV,FILNAM, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IDEVOT, + +SUM,WT,TOT,IDM,MAXLEN,LINE,CHRSET,DIALOG) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),FILNAM*(*),HELPF*(*),CHRSET(IDM) + CHARACTER LINE(MAXLEN) + INTEGER SUM(IDM,MAXLEN),TOT(MAXLEN) + INTEGER DIALOG + REAL WT(IDM,MAXLEN) + CALL SHOWFU(KBOUT,'Motif search using weight matrix') + CALL GETPAR(20,2,IOK,IOPT,IGON, + +IPAR3,IPAR4, + +IPAR5,IPAR6,IPAR7,IPAR8,IPAR9) + IF(IOK.NE.0) RETURN + CALL SIGNLD(IDEV,FILNAM, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH, + +SUM,IDM,MAXLEN,TOT,WT,LENGTH,MIDDLE,YMIN,YMIN1,YMAX, + +IGON,IOPT,IDEVOT,LINE,CHRSET,DIALOG,IOK) + IF(IOK.NE.0) RETURN + CALL PSIGNL(SEQ,IDIM,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,IDEVOT,WT,IDM,MAXLEN,LENGTH, + +YMIN,YMIN1,YMAX,IGON,MIDDLE) + END + SUBROUTINE SIGNLD(IDEV,FILNAM, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH, + +SUM,IDM,MAXLEN,TOT,WT,LENGTH,MIDDLE,YMIN,YMIN1,YMAX, + +IGON,IOPT,IDEVOT,LINE,CHRSET,DIALOG,IOK) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),HELPF*(*),CHRSET(IDM),LINE(MAXLEN) + INTEGER SUM(IDM,MAXLEN),TOT(MAXLEN),DIALOG + REAL WT(IDM,MAXLEN) + INTEGER VALUE + PARAMETER (MAXPRM = 21) + CHARACTER PROMPT(3)*(MAXPRM) + IOK = 1 + IDO = 1 + PROMPT(1) = 'Use weight matrix' + PROMPT(2) = 'Make weight matrix' + PROMPT(3) = 'Rescale weight matrix' + CALL RADION('Select operation',PROMPT,3,IDO, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IDO.LT.1) RETURN + IF(IDO.GT.1) THEN + CALL MKWT(WT,SUM,TOT,CHRSET,IDM,MAXLEN, + + IDEV,IDEVOT,KBIN,KBOUT,LINE, + + FILNAM,IDO,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IOK = 1 + RETURN + END IF + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + +'Motif weight matrix file', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + LENGTH=MAXLEN + CALL RDWMT(TOT,SUM,MIDDLE,LENGTH,MAXLEN,YMIN,YMAX,IDEV, + + IOK,IDM,KBOUT,0) + IF(IOK.NE.0)THEN + CALL ERROM(KBOUT,'Error in weight matrix') + RETURN + END IF + CALL YESONO(IOPT,'Use frequencies as weights', + +'Membership of set',IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOPT.LT.0) THEN + IOK = 1 + RETURN + END IF + IF(IOPT.EQ.1)THEN + CALL GETW1(SUM,WT,LENGTH,IDM,MAXLEN) + MININ = 1 + MAXIN = LENGTH + CALL GETINT(MININ,MAXIN,LENGTH,'Cutoff score', + + VALUE,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + YMIN = 0. + YMAX = LENGTH + YMIN1 = VALUE + ELSE + IF(DIALOG.EQ.1) THEN + AMN = -9999. + AMX = 9999. + CALL GETRL(AMN,AMX,YMIN,'Cutoff score',VAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + YMIN = VAL + END IF + IF(YMIN.LT.0.0)CALL GETW(TOT,SUM,WT,LENGTH,IDM,MAXLEN) + IF(YMIN.GE.0.0)CALL GETW2(SUM,WT,LENGTH,IDM,MAXLEN) + YMIN1 = YMIN + END IF + CALL YESONO(IGON,'Plot results','List results', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IGON.LT.0) RETURN + IOK = 0 + END + SUBROUTINE PSIGNL(SEQ,IDIM,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,IDEVOT,WT,IDM,MAXLEN,LENGTH, + +YMIN,YMIN1,YMAX,IGON,MIDDLE) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + REAL WT(IDM,MAXLEN) + INTEGER CTONUM + EXTERNAL CTONUM + XMAX=J2 + XMIN=J1 + IF(IGON.EQ.0)THEN + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + DO 10 I=J1,J2-LENGTH+1 + SUM1=0. + K=0 + DO 5 J=I,I+LENGTH-1 + K=K+1 + SUM1=SUM1+WT(CTONUM(SEQ(J)),K) +5 CONTINUE + IF(SUM1.GE.YMIN1)THEN + IF(IGON.EQ.0)THEN + X=I+MIDDLE + CALL LINE(X,X,YMIN,SUM1,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + IF(IGON.EQ.1)THEN + CALL VT100M + WRITE(IDEVOT,1010)I+MIDDLE,SUM1,(SEQ(K),K=I,I+LENGTH-1) +1010 FORMAT(' ',I7,F10.2,' ',120A1) + END IF + END IF +10 CONTINUE + CALL VT100M + END + SUBROUTINE LSTSEP(SEQ,IDIM,IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + INTEGER DIALOG + CALL SHOWFU(KBOUT,'List the sequence') + I1 = 1 + I2 = IDIM + LENGTH = 60 + IF(DIALOG.EQ.1) THEN + CALL LSTSPD(IDIM,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,I1,I2,LENGTH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL LSTSP(SEQ,IDIM,I1,I2,LENGTH,IDEV) + END + SUBROUTINE LSTSPD(IDIM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,I1,I2,LENGTH,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + INTEGER VALUE + IOK = 1 +1 CONTINUE + VALUE = I1 + CALL GETINT(I1,I2,VALUE, + +'List from ', + +VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0)RETURN + I1 = VALUE + VALUE = I2 + CALL GETINT(I1,I2,VALUE, + +'List to ', + +VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0)RETURN + I2 = VALUE + MININ = 10 + MAXIN = 120 + LENGTH = 60 + CALL GETINT(MININ,MAXIN,LENGTH, + +'Line length ', + +VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0)RETURN + IF(MOD(VALUE,10).NE.0)VALUE = LENGTH + LENGTH = VALUE + END + SUBROUTINE LSTSP(SEQ,IDIM,I1,I2,LENGTH,IDEV) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + CALL FMTSEP(SEQ,IDIM,I1,I2,LENGTH,IDEV) + END + SUBROUTINE FMTSEP(SEQ1,IDIM,ISW,ISE,LINLEN,IDEV) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM) + INTEGER KL(12) + ISWW=ISW-1 + IE=ISW-1 +1 CONTINUE + WRITE(IDEV,1003) +1003 FORMAT( ) +C SET UP DECIMAL COUNTERS + DO 50 J=1,LINLEN/10 + ISWW=ISWW+10 + KL(J)=ISWW +50 CONTINUE + IS=IE+1 + IE=IE+LINLEN + IF(IE.GT.ISE)IE=ISE + WRITE(IDEV,1001)(KL(KKK),KKK=1,MIN(IE-IS+1,LINLEN)/10) + WRITE(IDEV,1002)(SEQ1(K),K=IS,IE) +1002 FORMAT( ' ',12(10A1,1X)) +1001 FORMAT( ' ',12(5X,I6)) + IF(IE.EQ.ISE)RETURN + GO TO 1 + END +C ROUTINE TO PERFORM ROBSON SECONDARY STRUCTURE PREDICTION FOR PROTEINS + SUBROUTINE ROBSON(SEQ,IDIM1, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KBIN,KBOUT, + +IDEV,FILNAM, + +IHELPS,IHELPE,HELPF,IDEVH,IDEVOT,DIALOG) +C AUTHOR: RODGER STADEN + INTEGER DIALOG + CHARACTER HELPF*(*),FILNAM*(*) + CHARACTER SEQ(IDIM1) + INTEGER HELIX(-8:8,26),EXTEND(-8:8,26),TURN(-8:8,26),COIL(-8:8,26) + CALL SHOWFU(KBOUT,'Plot Robson secondary structure predictions') + CALL OPENRS(IDEV,FILNAM,IOK,LRECL,2) + IF(IOK.NE.0)THEN + CALL ERROM(KBOUT,'Error opening Robson weights file') + RETURN + END IF + CALL REDROB(HELIX,IDEV,IFAIL) + CALL REDROB(EXTEND,IDEV,IFAIL) + CALL REDROB(TURN,IDEV,IFAIL) + CALL REDROB(COIL,IDEV,IFAIL) + CLOSE(UNIT=IDEV) + IF(IFAIL.NE.0)THEN + CALL ERROM(KBOUT,'Error in Robson score matrix') + RETURN + END IF + CALL GETPAR(24,4,IOK,MINIW,MAXIW,IWRIT,IGON, + +IPAR5,IPAR6,IPAR7,IPAR8,IPAR9) + IF(IOK.NE.0) RETURN + IF(DIALOG.EQ.1) THEN + CALL ROBSND(MINIW,MAXIW,IWRIT, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IGON,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL ROBSNP(SEQ,IDIM1, + +HELIX,EXTEND,TURN,COIL, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2, + +IDEVOT,IWRIT,IGON) + END + SUBROUTINE ROBSNP(SEQ,IDIM1, + +HELIX,EXTEND,TURN,COIL, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2, + +IDEVOT,IWRIT,IGON) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1) + INTEGER CTONUM + INTEGER HELIX(-8:8,26),EXTEND(-8:8,26),TURN(-8:8,26),COIL(-8:8,26) + EXTERNAL CTONUM + YMINH=-400. + YMAXH=400. + YMINE=-400. + YMAXE=400. + YMINT=-400. + YMAXT=400. + YMINC=-400. + YMAXC=400. + YMEAN = 0. + YMIND=0.0 + YMAXD=100.0 + YHD=20. + YED=40. + YTD=60. + YCD=80. + XMIN=J1 + XMAX=J2 + YMID=YMINH+(YMAXH-YMINH)*0.75 + XMARG=REAL(MARGT)/5.0 + IMARG=NINT(XMARG) + MARGBH=MARGB+XMARG + MARGTH=IMARG + MARGBE=MARGB+2*XMARG + MARGTE=IMARG + MARGBT=MARGB+3*XMARG + MARGTT=IMARG + MARGBC=MARGB+4*XMARG + MARGTC=IMARG + MARGBD=MARGB + MARGTD=IMARG + NH = 0 + NE = 0 + NT = 0 + NC = 0 + IF(IGON.EQ.0)THEN + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGBH,MARGTH,ISXMAX,ISYMAX) + CALL FRAME(MARGL,MARGR,MARGBE,MARGTE,ISXMAX,ISYMAX) + CALL FRAME(MARGL,MARGR,MARGBT,MARGTT,ISXMAX,ISYMAX) + CALL FRAME(MARGL,MARGR,MARGBC,MARGTC,ISXMAX,ISYMAX) + CALL FRAME(MARGL,MARGR,MARGBD,MARGTD,ISXMAX,ISYMAX) + CALL TEXT(XMIN,YMID,' H',2,0,XMAX,XMIN,YMAXH,YMINH, + 1MARGL,MARGR,MARGBH,MARGTH,ISXMAX,ISYMAX) + CALL TEXT(XMIN,YMID,' E',2,0,XMAX,XMIN,YMAXE,YMINE, + 1MARGL,MARGR,MARGBE,MARGTE,ISXMAX,ISYMAX) + CALL TEXT(XMIN,YMID,' T',2,0,XMAX,XMIN,YMAXT,YMINT, + 1MARGL,MARGR,MARGBT,MARGTT,ISXMAX,ISYMAX) + CALL TEXT(XMIN,YMID,' C',2,0,XMAX,XMIN,YMAXC,YMINC, + 1MARGL,MARGR,MARGBC,MARGTC,ISXMAX,ISYMAX) + CALL TEXT(XMIN,YCD,' D',2,0,XMAX,XMIN,YMAXD,YMIND, + 1MARGL,MARGR,MARGBD,MARGTD,ISXMAX,ISYMAX) + END IF + IDONE=0 + XP=MAX(J1,9) + X=XP + YPH=0. + YPE=0. + YPC=0. + YPT=0. + IOPT = 1 + DO 10 I=MAX(J1,9),MIN(J2,IDIM1-8) + IDONE=IDONE+1 + SUMH=0. + SUME=0. + SUMC=0. + SUMT=0. + K=I-9 + DO 5 J=8,-8,-1 + K=K+1 + ISEQ=CTONUM(SEQ(K)) + SUMH=SUMH+HELIX(J,ISEQ) + SUME=SUME+EXTEND(J,ISEQ) + SUMC=SUMC+COIL(J,ISEQ) + SUMT=SUMT+TURN(J,ISEQ) +5 CONTINUE + IF(MOD(IDONE,IWRIT).EQ.0)THEN + YMAX=MAX(SUMH,SUME,SUMC,SUMT) + X=I + IF(IGON.EQ.0)THEN + CALL LINE(XP,X,YPH,SUMH,XMAX,XMIN,YMAXH,YMINH, + + MARGL,MARGR,MARGBH,MARGTH,ISXMAX,ISYMAX) + IF(YMAX.EQ.SUMH)THEN + CALL POINT(X,YHD,XMAX,XMIN,YMAXD,YMIND, + + MARGL,MARGR,MARGBD,MARGTD,ISXMAX,ISYMAX) + CALL POINT(X,YMEAN,XMAX,XMIN,YMAXH,YMINH, + + MARGL,MARGR,MARGBH,MARGTH,ISXMAX,ISYMAX) + NH = NH + 1 + END IF + YPH=SUMH + CALL LINE(XP,X,YPE,SUME,XMAX,XMIN,YMAXE,YMINE, + + MARGL,MARGR,MARGBE,MARGTE,ISXMAX,ISYMAX) + IF(YMAX.EQ.SUME)THEN + CALL POINT(X,YED,XMAX,XMIN,YMAXD,YMIND, + + MARGL,MARGR,MARGBD,MARGTD,ISXMAX,ISYMAX) + CALL POINT(X,YMEAN,XMAX,XMIN,YMAXE,YMINE, + + MARGL,MARGR,MARGBE,MARGTE,ISXMAX,ISYMAX) + NE = NE + 1 + END IF + YPE=SUME + CALL LINE(XP,X,YPT,SUMT,XMAX,XMIN,YMAXT,YMINT, + + MARGL,MARGR,MARGBT,MARGTT,ISXMAX,ISYMAX) + IF(YMAX.EQ.SUMT)THEN + CALL POINT(X,YTD,XMAX,XMIN,YMAXD,YMIND, + + MARGL,MARGR,MARGBD,MARGTD,ISXMAX,ISYMAX) + CALL POINT(X,YMEAN,XMAX,XMIN,YMAXT,YMINT, + + MARGL,MARGR,MARGBT,MARGTT,ISXMAX,ISYMAX) + NT = NT + 1 + END IF + YPT=SUMT + CALL LINE(XP,X,YPC,SUMC,XMAX,XMIN,YMAXC,YMINC, + + MARGL,MARGR,MARGBC,MARGTC,ISXMAX,ISYMAX) + IF(YMAX.EQ.SUMC)THEN + CALL POINT(X,YCD,XMAX,XMIN,YMAXD,YMIND, + + MARGL,MARGR,MARGBD,MARGTD,ISXMAX,ISYMAX) + CALL POINT(X,YMEAN,XMAX,XMIN,YMAXC,YMINC, + + MARGL,MARGR,MARGBC,MARGTC,ISXMAX,ISYMAX) + NC = NC + 1 + END IF + YPC=SUMC + XP=I + END IF + IF(IGON.EQ.1)THEN + CALL VT100M + WRITE(IDEVOT,1005) + + I,SEQ(I),INT(SUMH),INT(SUME),INT(SUMT),INT(SUMC) +1005 FORMAT(' ',I6,' ',A1,' ',4I5) + IF(IGON.EQ.0)CALL VECTOM + END IF + END IF +10 CONTINUE + CALL VT100M + WRITE(IDEVOT,1006)NH,NE,NT,NC +1006 FORMAT( + +' Helix =',I6,', Extended =',I6,', Turn =',I6,', Coil =',I6) + END + SUBROUTINE ROBSND(MINIW,MAXIW,IWRIT, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IGON,IOK) + CHARACTER HELPF*(*) + IOK = 1 + CALL YESONO(IGON,'Plot results','List results', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IGON.LT.0) RETURN + IF(IGON.EQ.0) THEN + CALL GETINT(MINIW,MAXIW,IWRIT,'Plot interval',IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IWRIT = IVAL + END IF + IOK = 0 + END + SUBROUTINE REDROB(TABLE,IDEV,IFAIL) +C AUTHOR: RODGER STADEN + INTEGER TABLE(-8:8,26) +C SET FAIL FLAG + IFAIL=1 +1000 FORMAT( ) +1001 FORMAT(3X,17I4) +C READ TITLE + READ(IDEV,1000,END=100,ERR=100) +C READ POSITIONS + READ(IDEV,1000,END=100,ERR=100) +C READ VALUES + DO 10 I=1,26 + READ(IDEV,1001,ERR=100,END=100)(TABLE(K,I),K=-8,8) +10 CONTINUE +C SET FAIL FLAG TO GOOD + IFAIL=0 + RETURN +100 CONTINUE + RETURN + END +C SUBROUTINE TO DRAW HELIX WHEELS + SUBROUTINE HELIXW(SEQ,IDSEQ,KSTART,J1,J2,IX0,IXLEN,IY0,IYLEN, + +ISXMAX,ISYMAX,KBIN,KBOUT,HYDRO,IDSCOR, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),HELPF*(*) + INTEGER DIALOG + INTEGER WINDOW + REAL HYDRO(IDSCOR) + PARAMETER (DEGTOR = 0.017453) + CALL SHOWFU(KBOUT,'Draw helix wheel') + CALL GETPAR(26,6,IOK,MINANG,MAXANG,IANGLE,MINIW,MAXIW,WINDOW, + +IPAR7,IPAR8,IPAR9) + IF(IOK.NE.0) RETURN + IF(DIALOG.EQ.1) THEN + CALL HELIXD(MINANG,MAXANG,IANGLE,MINIW,MAXIW,WINDOW, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + END IF + ANGLE = REAL(IANGLE)*DEGTOR + CALL HELIXP(SEQ,IDSEQ,KSTART,J1,J2,IX0,IXLEN,IY0,IYLEN, + +ISXMAX,ISYMAX,KBIN,KBOUT,HYDRO,IDSCOR, + +IHELPS,IHELPE,HELPF,IDEVH,WINDOW,ANGLE) + END + SUBROUTINE HELIXP(SEQ,IDSEQ,KSTART,J1,J2,IX0,IXLEN,IY0,IYLEN, + +ISXMAX,ISYMAX,KBIN,KBOUT,HYDRO,IDSCOR, + +IHELPS,IHELPE,HELPF,IDEVH,WINDOW,ANGLE) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),HELPF*(*) + CHARACTER SYMBOL*26,ATOS*60,SYMT*2,POSN*6,POSNHP*8 + CHARACTER*2 NUMBER(1:60) + INTEGER CTONUM,WINDOW + REAL HYDRO(IDSCOR) + EXTERNAL CTONUM,ATOS + PARAMETER ( + + RAD1 = 0.78, + + RAD2 = 1.25, + + RAD3 = 1.50, + + RAD4 = 1.0, + + XMIN = -1.75, + + XMAX = 1.75, + + YMIN = -1.75, + + YMAX = 1.75, + + CENTRX = 0.0, + + CENTRY = 0.0, + + TXMIN = XMIN + (XMAX-XMIN)*0.05, + + TYMIN = YMAX - (YMAX-YMIN)*0.04, + + PXMIN = XMIN + (XMAX-XMIN)*0.05, + + PYMIN = YMAX - (YMAX-YMIN)*0.16, + + HMXMIN = XMIN + (XMAX-XMIN)*0.05, + + HMYMIN = YMAX - (YMAX-YMIN)*0.08, + + HXMIN = XMIN + (XMAX-XMIN)*0.05, + + HYMIN = YMAX - (YMAX-YMIN)*0.12) +C + TXMIN = XMIN + (XMAX-XMIN)*0.05, +C + TYMIN = YMAX - (YMAX-YMIN)*0.03, +C + PXMIN = XMIN + (XMAX-XMIN)*0.05, +C + PYMIN = YMAX - (YMAX-YMIN)*0.14, +C + HMXMIN = XMIN + (XMAX-XMIN)*0.05, +C + HMYMIN = YMAX - (YMAX-YMIN)*0.06, +C + HXMIN = XMIN + (XMAX-XMIN)*0.05, +C + HYMIN = YMAX - (YMAX-YMIN)*0.1) + PARAMETER (DELTA = 0.001) +C + SYMBOL =' -- +++ ...... ' + DO 1 I = 1,60 + WRITE(NUMBER(I),1005)I +1 CONTINUE +1005 FORMAT(I2) + POSNHP=' ' +C +C SEQUENCE CHARACTER IN ELEMENT 1 OF SEQ ARRAY IS KSTART +C FIRST SEQUENCE CHARACTER IN ACTIVE REGION IS J1, LAST J2 +C DIMENSION OF SEQ ARRAY IS IDSEQ +C ELEMENT NUMBER FOR J1 IS J1-KSTART+1 +C ELEMENT NUMBER FOR J2 IS J2-KSTART+1 +C NUMBER OF ELEMENTS TO PROCESS IS J2-J1+1 +C WORK IN ELEMENT NUMBERS AND SET TO SEQUENCE NUMBERS FOR OUTPUT +C SET FIRST ELEMENT NUMBER + I1 = J1-KSTART+1 +C +C +10 CONTINUE +C +C + CALL CLEARV + CALL VECTOM + CALL CLEARG + CALL VECTOM + CALL FRAME(IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) + XF = 0.0 + YF = RAD1 + ICLOCK = 0 + I2 = MIN(I1+WINDOW-1,J2-KSTART+1) + IWIN = I2-I1+1 + DO 20 I = I1,I2 + ICLOCK = ICLOCK + 1 + TX = COS(1.5708-((I-I1)*ANGLE)) + TY = SIN(1.5708-((I-I1)*ANGLE)) + X = RAD2*TX + Y = RAD2*TY + SYMT = SEQ(I) + CALL TEXT(X,Y,SYMT,1,ISIZE,XMAX,XMIN,YMAX,YMIN, + + IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) + X = RAD4*TX + Y = RAD4*TY + SYMT = NUMBER(ICLOCK) + CALL TEXT(X,Y,SYMT,2,ISIZE,XMAX,XMIN,YMAX,YMIN, + + IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) + X = RAD3*TX + Y = RAD3*TY + K = CTONUM(SEQ(I)) + SYMT = SYMBOL(K:K) + CALL TEXT(X,Y,SYMT,1,ISIZE,XMAX,XMIN,YMAX,YMIN, + + IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) + X = RAD1*TX + Y = RAD1*TY + CALL LINE(XF,X,YF,Y,XMAX,XMIN,YMAX,YMIN, + + IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) + XF = X + YF = Y + I3 = I +20 CONTINUE + CALL EISEN(SEQ(I1),IWIN,HYDRO,IDSCOR,ANGLE,HM,H) + CALL TEXT(TXMIN,TYMIN,ATOS(SEQ(I1),IWIN),IWIN,ISIZE, + + XMAX,XMIN,YMAX,YMIN, + + IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) + WRITE(POSN,1000)I1+KSTART-1 +1000 FORMAT(I6) + CALL TEXT(PXMIN,PYMIN,POSN,6,ISIZE, + + XMAX,XMIN,YMAX,YMIN, + + IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) + WRITE(POSN,1001)HM*WINDOW +1001 FORMAT(F6.2) + POSNHP(2:) = POSN + POSNHP(1:1) = 'M' + CALL TEXT(HMXMIN,HMYMIN,POSNHP,8,ISIZE, + + XMAX,XMIN,YMAX,YMIN, + + IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) + WRITE(POSN,1001)H*WINDOW + POSNHP(2:) = POSN + POSNHP(1:1) = 'H' + CALL TEXT(HXMIN,HYMIN,POSNHP,8,ISIZE, + + XMAX,XMIN,YMAX,YMIN, + + IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) + ICLOCK = ICLOCK + 1 + TX = COS(1.5708-((I3+1-I1)*ANGLE)) + TY = SIN(1.5708-((I3+1-I1)*ANGLE)) + X = RAD1*TX + Y = RAD1*TY + IF((ABS(X).LT.DELTA).AND.(ABS(Y-RAD1).LT.DELTA)) + + CALL LINE(XF,0.0,YF,RAD1,XMAX,XMIN,YMAX,YMIN, + + IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) + CALL FLUSHG +30 CONTINUE + CALL VT100M + MINSTP = -1000 + MAXSTP = 1000 + NEXT = 1 + CALL GETINT(MINSTP,MAXSTP,NEXT,'Step', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) GO TO 40 + NEXT = IVAL + I1 = I1 + NEXT + IF(I1.LT.0)GO TO 40 + IF(I1.GT.J2-KSTART+1)GO TO 40 + IF(I1.LT.J1-KSTART+1)GO TO 40 + GO TO 10 +40 CONTINUE + CALL VT100M + END + SUBROUTINE HELIXD(MINANG,MAXANG,IANGLE,MINIW,MAXIW,WINDOW, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + INTEGER WINDOW + IOK = 1 + CALL GETINT(MINANG,MAXANG,IANGLE,'Angle',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IANGLE = IVAL + CALL GETINT(MINIW,MAXIW,WINDOW,'Window',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + WINDOW = IVAL + END +C SUBROUTINE TO PLOT HYDROPHOBIC MOMENTS + SUBROUTINE HYDMOM(SEQ,IDSEQ,HYDRO,IDSCOR, + +IX0,IXLEN,IY0,IYLEN, + +ISXMAX,ISYMAX,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),HELPF*(*) + INTEGER DIALOG + INTEGER WINDOW + REAL HYDRO(IDSCOR) + PARAMETER (DEGTOR = 0.017453) + EXTERNAL CTONUM + CALL SHOWFU(KBOUT,'Plot hydrophobic moment') + CALL GETPAR(25,9,IOK,MINANG,MAXANG,IANGLE,MINWIN,MAXWIN,WINDOW, + +MINIW,MAXIW,IWRIT) + IF(IOK.NE.0) RETURN + IF(DIALOG.EQ.1) THEN + CALL HYDMD(MINANG,MAXANG,IANGLE,MINWIN,MAXWIN,WINDOW, + + MINIW,MAXIW,IWRIT, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + END IF + ANGLE = REAL(IANGLE)*DEGTOR + CALL HYDMP(SEQ,IDSEQ,HYDRO,IDSCOR, + +IX0,IXLEN,IY0,IYLEN, + +ISXMAX,ISYMAX,WINDOW,ANGLE,IWRIT) + END + SUBROUTINE HYDMP(SEQ,IDSEQ,HYDRO,IDSCOR, + +IX0,IXLEN,IY0,IYLEN, + +ISXMAX,ISYMAX,WINDOW,ANGLE,IWRIT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ) + INTEGER CTONUM,WINDOW + REAL HYDRO(IDSCOR) + EXTERNAL CTONUM + XMIN = 1 + XMAX = IDSEQ + CALL CLEARV + CALL VECTOM + Y = REAL(IYLEN)/2. + IYLEN1 = NINT(Y) + IY1 = IY0 + IYLEN1 + CALL FRAME(IX0,IXLEN,IY0,IYLEN1,ISXMAX,ISYMAX) + CALL FRAME(IX0,IXLEN,IY1,IYLEN1,ISXMAX,ISYMAX) + IBH = 150 + CALL SCALES(XMAX,XMIN,1.5,-1.0, + 1 IX0,IXLEN,IY1,IYLEN1,ISXMAX,ISYMAX, + 1 0.5,IBH,-1.0,2) + CALL SCALES(XMAX,XMIN,1.5,0.0, + 1 IX0,IXLEN,IY0,IYLEN1,ISXMAX,ISYMAX, + 1 0.5,IBH,0.0,2) + IBH = 50 + CALL SCALES(XMAX,XMIN,1.5,-1.0, + 1 IX0,IXLEN,IY1,IYLEN1,ISXMAX,ISYMAX, + 1 0.1,IBH,-1.0,2) + CALL SCALES(XMAX,XMIN,1.5,0.0, + 1 IX0,IXLEN,IY0,IYLEN1,ISXMAX,ISYMAX, + 1 0.1,IBH,0.0,2) +C CALL TEXT(2.,1.4,'HYDROPHOBIC MOMENT',18,ISIZE, +C + 100.0,1.0,1.5,0.0, +C + IX0,IXLEN,IY0,IYLEN1,ISXMAX,ISYMAX) +C CALL TEXT(2.0,1.3,'HYDROPHOBICITY',14,ISIZE, +C + 100.,1.0,1.5,-1.0, +C + IX0,IXLEN,IY1,IYLEN1,ISXMAX,ISYMAX) +C CALL LINE(1.,XMAX,0.55,0.55,XMAX,XMIN,1.5,-1.0, +C + IX0,IXLEN,IY1,IYLEN1,ISXMAX,ISYMAX) +C CALL LINE(1.,XMAX,0.68,0.68,XMAX,XMIN,1.5,-1.0, +C + IX0,IXLEN,IY1,IYLEN1,ISXMAX,ISYMAX) +C CALL LINE(1.,XMAX,0.42,0.42,XMAX,XMIN,1.5,-1.0, +C + IX0,IXLEN,IY1,IYLEN1,ISXMAX,ISYMAX) + IDONE = 0 + XF = 0. + HMF = 0. + HF = 0. + DO 40 J = 1,IDSEQ-WINDOW+1 + I1 = J + CALL EISEN(SEQ(I1),WINDOW,HYDRO,IDSCOR,ANGLE,HM,H) + IDONE = IDONE + 1 + IF(MOD(IDONE,IWRIT).EQ.0)THEN + X = I1 + CALL LINE(XF,X,HMF,HM,XMAX,XMIN,1.5,0.0, + + IX0,IXLEN,IY0,IYLEN1,ISXMAX,ISYMAX) + CALL LINE(XF,X,HF,H,XMAX,XMIN,1.5,-1.0, + + IX0,IXLEN,IY1,IYLEN1,ISXMAX,ISYMAX) + XF = X + HMF = HM + HF = H + END IF +C +40 CONTINUE + CALL VT100M + END + SUBROUTINE HYDMD(MINANG,MAXANG,IANGLE,MINWIN,MAXWIN,WINDOW, + +MINIW,MAXIW,IWRIT, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + INTEGER WINDOW + IOK = 1 + CALL GETINT(MINANG,MAXANG,IANGLE,'Angle',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IANGLE = IVAL + CALL GETINT(MINWIN,MAXWIN,WINDOW,'Window',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + WINDOW = IVAL + CALL GETINT(MINIW,MAXIW,IWRIT,'Plot interval',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IWRIT = IVAL + IOK = 0 + END + SUBROUTINE EISEN(SEQ,WINDOW,HYDRO,IDHYD,DELTA,HM,H) + INTEGER WINDOW + CHARACTER SEQ(WINDOW) + REAL HYDRO(IDHYD) + INTEGER CTONUM + EXTERNAL CTONUM +C AUTHOR RODGER STADEN +C CALCULATES THE MEAN HYDROPHOBICITY AND MEAN HYDROPHOBIC MOMENT +C AFTER EISENBERG, FOR A WINDOW OF SIZE WINDOW, AND ANGLE DELTA + T1 = 0. + T2 = 0. + H = 0. + DO 20 I = 1, WINDOW +C + IT =CTONUM(SEQ(I)) + T = HYDRO(IT) + T3 = DELTA * I - 1 + T1 = T1 + T * SIN(T3) + T2 = T2 + T * COS(T3) + H = H + T +C +20 CONTINUE + T1 = T1 * T1 + T2 = T2 * T2 + HM = T1 + T2 + IF(HM.GT.0.0)HM = SQRT(HM) + H = H / WINDOW + HM = HM / WINDOW + END +C BACK + SUBROUTINE BACK(SEQ,IDSEQ,CODIN,KBIN,KBOUT,FILNAM,IDEV, + +DNA,MAXSEQ,PCOD,IDIM1P,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),DNA(MAXSEQ),FILNAM*(*),PAA(5,5,5) + CHARACTER*3 CODON(26),CODIN(26) + REAL CODS(4,4,4),PROB(26),PCOD(IDIM1P) + CHARACTER HELPF*(*) + INTEGER DIALOG + INTEGER SPAN + SAVE PAA + DATA PAA/'F','F','L','L','-','S','S','S','S','S', + 1'Y','Y','*','*','-','C','C','*','W','-', + 1'-','-','-','-','-','L','L','L','L','L', + 1'P','P','P','P','P','H','H','Q','Q','-', + 1'R','R','R','R','R','-','-','-','-','-','I','I','I','M','-', + 1'T','T','T','T','T', + 1'N','N','K','K','-','S','S','R','R','-','-','-','-','-','-', + 1'V','V','V','V','V','A','A','A','A','A','D','D','E','E','-', + 1'G','G','G','G','G', + 1'-','-','-','-','-','-','-','-','-','-', + 1'-','-','-','-','-','-','-','-','-','-', + 1'-','-','-','-','-','-','-','-','-','-'/ + CALL GETPAR(27,8,IOK,IOP,IPLOT,MINWIN,MAXWIN,SPAN, + +MINIW,MAXIW,IWRIT,IPAR9) + IF(IOK.NE.0) RETURN + CALL SHOWFU(KBOUT,'Back translate') + DO 10 I = 1,26 + CODON(I) = CODIN(I) +10 CONTINUE + CALL FILLR(PROB,26,0.) + CALL FILLR(CODS,64,0.) +C GET CODON TABLE FROM FILE IF REQUIRED + IF(DIALOG.EQ.1) THEN + CALL BACKD1(IOP,IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT, + + FILNAM,IDEV,CODS,PAA,IOK) + IF(IOK.NE.0) RETURN + END IF +C BACK TRANSLATE + CALL BACKA(CODS,PAA,CODON,PROB,PCOD,IDIM1P,MXSPAN, + +SEQ,IDSEQ,DNA,MAXSEQ) +C SET UP PLOTTING + IF(DIALOG.EQ.1) THEN + CALL BACKD2(IPLOT,MINWIN,MAXWIN,SPAN,MINIW,MAXIW,IWRIT, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + IF(IPLOT.LT.0) RETURN + END IF + IF(IPLOT.EQ.0)THEN +C PLOT REDUNDANCY + CALL PLOTRD(PCOD,IDIM1P,MXSPAN,1,IDSEQ, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,SPAN,IWRIT) + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + END IF + IDIM = 3*IDSEQ +C SAVE TO DISK + CALL BACKO(DNA,IDIM,FILNAM,IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + END + SUBROUTINE BACKD2(IPLOT,MINWIN,MAXWIN,SPAN,MINIW,MAXIW,IWRIT, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + INTEGER SPAN + CALL YESNO(IPLOT,'Plot redundancy', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IPLOT.LT.0)RETURN + IF(IPLOT.EQ.0) THEN + CALL GSPIN(MINWIN,MAXWIN,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + END + SUBROUTINE BACKO(DNA,IDIM,FILNAM,IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER DNA(IDIM) + CHARACTER FILNAM*(*),HELPF*(*) + CALL YESNO(IN,'Save DNA to disk', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IN.LT.0)RETURN + IF(IN.EQ.0)THEN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,1,IOK,KBIN,KBOUT, + + 'File name for DNA sequence', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.EQ.0)THEN + CALL FMTDK(IDEV,DNA,IDIM) + CLOSE(UNIT=IDEV) + END IF + END IF + END + SUBROUTINE BACKD1(IOP,IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT, + +FILNAM,IDEV,CODS,PAA,IOK) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),HELPF*(*),PAA(5,5,5) + REAL CODS(4,4,4) + IOK = 1 + CALL YESONO(IOP,'No codon preference', + +'Use file of codon preferences', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) RETURN + IF(IOP.EQ.0) THEN + IOK = 0 + RETURN + END IF + IF(IOP.EQ.1)THEN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + + 'Codon table file name', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.EQ.0)THEN + CALL REDCOD(CODS,IDEV) + CLOSE(UNIT=IDEV) + CALL WRTCOD(CODS,KBOUT,PAA) + END IF + END IF + END + SUBROUTINE BACKA(CODS,PAA,CODON,PROB,PCOD,IDIM1P,MXSPAN, + +SEQ,IDSEQ,DNA,MAXSEQ) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),DNA(MAXSEQ),PAA(5,5,5) + CHARACTER*3 CODON(26) + REAL CODS(4,4,4),PROB(26),PCOD(IDIM1P) + INTEGER CTONUM + EXTERNAL CTONUM +C USE TOP CODONS + CALL TOPCOD(CODS,PAA,CODON,PROB) +C BACK TRANSLATE + I1 = 1+MXSPAN/2 + CALL FILLR(PCOD,I1,0.) + DO 100 I = 1,IDSEQ + K = CTONUM(SEQ(I)) + DNA(1+3*(I-1)) = CODON(K)(1:1) + DNA(2+3*(I-1)) = CODON(K)(2:2) + DNA(3+3*(I-1)) = CODON(K)(3:3) + PCOD(I+I1) = PROB(K) +100 CONTINUE + CALL FILLR(PCOD(IDSEQ+I1+1),I1,0.) + END +C CHECK EACH AMINO ACID IN TURN FOR EVEN CODON USE + SUBROUTINE TOPCOD(CODUSE,PAA,CODON,PROB) +C AUTHOR: RODGER STADEN + CHARACTER PAA(5,5,5),AA(22),BASE(4) + CHARACTER*3 CODON(26) + REAL CODUSE(4,4,4),PROB(26) + SAVE AA,BASE + DATA AA/'C','S','T','P','A','G','N','D','E','Q','B', + +'Z','H','R','K','M','I','L','V','F','Y','W'/ + DATA BASE/'T','C','A','G'/ +C IF CODONS FOR AN ACID ARE EVENLY USED EMPLOY ALL CODONS +C OTHERWISE EMPLOY THE TOP SCORER. CHECK THE USAGE +C LOOP FOR EACH ACID + DO 300 L=1,22 + IMAX = 0 + JMAX = 0 + KMAX = 0 + AMAX=0.0 + NCOD = 0 + TCOD = 0.0 + IEVEN = 1 +C LOOP FOR EACH CODON + DO 200 I=1,4 + DO 200 J=1,4 + DO 200 K=1,4 +C EXAMINE CODONS FOR ACID L + IF(PAA(K,J,I).EQ.AA(L))THEN + NCOD = NCOD + 1 + TCOD = TCOD + CODUSE(I,J,K) + IF(CODUSE(I,J,K).GT.AMAX)THEN + AMAX = CODUSE(I,J,K) + IMAX = I + JMAX = J + KMAX = K + IEVEN = 0 + ELSE IF(CODUSE(I,J,K).EQ.AMAX)THEN + IEVEN = 1 + END IF + END IF +200 CONTINUE +C IF THERE IS A PREFERENCE, USE IT + IF((AMAX.GT.0.0).AND.(IEVEN.EQ.0))THEN + CODON(L) = BASE(IMAX)//BASE(JMAX)//BASE(KMAX) + PROB(L) = AMAX/TCOD + ELSE + PROB(L) = 1.0/(MAX(1,NCOD)) + END IF +300 CONTINUE + END + SUBROUTINE PLOTRD(SEQ,IDIM1P,MXSPAN,J1,J2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,SPAN,IWRIT) +C AUTHOR: RODGER STADEN + REAL SEQ(IDIM1P) + INTEGER SPAN +C length forward and back + LF=SPAN/2 + LB=1+SPAN/2 +C max score + SCRMAX=1. + SCRMIN=0.16 + I1INM1=1+MXSPAN/2 + J1P=J1+I1INM1 + J2P=J2+I1INM1 + XMIN=J1 + XMAX=J2 +C set ymax + YMIN=SPAN*SCRMIN + YMAX=SPAN*SCRMAX +C do edge + SUM=0. + DO 90 I=J1P-LB,J1P-LB+SPAN + SUM=SUM+SEQ(I) +90 CONTINUE + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C do rest + IDONE=0 + XF=J1 + YF=SUM + DO 100 I=J1P,J2P + IDONE=IDONE+1 + SUM=SUM+SEQ(I+LF)-SEQ(I-LB) + IF(MOD(I,IWRIT).EQ.0)THEN + XT=I-I1INM1 + CALL LINE(XF,XT,YF,SUM,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XF=XT + YF=SUM + END IF +100 CONTINUE + CALL VT100M + END + SUBROUTINE FIND8(SEQ,IDIM1,STRING,IDIM2,INC,IMATCH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER PSEQ,PSTR + INTEGER CTONUM + EXTERNAL CTONUM + PSEQ=1-1*INC + PSTR=1 + IMATCH=0 +100 CONTINUE + PSEQ=PSEQ+1-PSTR + PSEQ=PSEQ+INC-1 + PSTR=0 +500 CONTINUE + PSEQ=PSEQ+1 + IF(PSEQ.GT.IDIM1)RETURN + PSTR=PSTR+1 + KSTRNG = CTONUM(STRING(PSTR)) + KSEQ = CTONUM(SEQ(PSEQ)) + IF((KSTRNG.NE.KSEQ).AND.(KSTRNG.NE.23)) GO TO 100 + IF(PSTR.LT.IDIM2)GO TO 500 + IMATCH=PSEQ-IDIM2+1 + END + SUBROUTINE SQPFIT(SEQNCE,IDIM1,SEQ2,IDIM3I,ITOT,ITOTEL,ITOTID, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART, + +IDEV,IDEVIN,FILE2,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,DIALOG) +C AUTHOR: RODGER STADEN + INTEGER DIALOG + PARAMETER (MAXSTR = 50) + CHARACTER HELPF*(*) + CHARACTER SEQNCE(IDIM1),STRING(MAXSTR) + CHARACTER SEQ2(IDIM3I),FILE2*(*) + CHARACTER MATCH(MAXSTR) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + INTEGER ANSTY + CALL SHOWFU(KBOUT,'Find percentage matches') + IDIM2 = 0 + IDIM2I = MAXSTR + IDIM3 = IDIM3I + IS = J1 + IE = J2 + ISS = 1 + ISE = 10 + CALL GETPAR(18,2,IOK,ANSTY,IPR, + +IPAR3,IPAR4, + +IPAR5,IPAR6,IPAR7,IPAR8,IPAR9) + IF(IOK.NE.0) RETURN + PR = REAL(IPR) +5 CONTINUE + IF(DIALOG.EQ.1) THEN + CALL SQPFD1(SEQ2,IDIM3I,IDIM3,ANSTY,IDEVIN,FILE2, + + KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF +10 CONTINUE + CALL SQPFD2(SEQ2,IDIM3,MATCH,STRING, + +J1,J2,KSTART,ISS,ISE,IS,IE,IDIM2I,IDIM2,MAXSTR,PR,ANSTY,MINP, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL SQPF6(SEQNCE,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,KSTART,J1,J2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IOK) + IF(IOK.EQ.2)THEN + WRITE(KBOUT,1017)ITOTID +1017 FORMAT(/,' more than',I6,' matches.', + + ' try changing percentage or region'/) + GO TO 10 + END IF + IF(IOK.NE.0) RETURN + CALL SQPF7(SEQNCE,IDIM1,STRING,IDIM2,MATCH,ITOT,ITOTEL,ITOTID, + +ITOTP,KSTART,J1,J2,ISS,PR,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + GO TO 10 + END + SUBROUTINE SQPFD2(SEQ2,IDIM3,MATCH,STRING, + +J1,J2,KSTART,ISS,ISE,IS,IE,IDIM2I,IDIM2,MAXSTR,PR,ANSTY,MINP, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + CHARACTER STRING(MAXSTR) + CHARACTER SEQ2(IDIM3) + CHARACTER MATCH(MAXSTR) + INTEGER ANSC,ANSTY + IOK = 1 + CALL YESNO(ANSC,'Keep picture', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANSC.LT.0)RETURN + IF(ANSC.EQ.1)CALL CLEARG + IF(ANSTY.EQ.1) THEN + CALL SQPF2(SEQ2,IDIM3,STRING,IDIM2I,IDIM2,ISS,ISE, + + KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + IF(ANSTY.EQ.0) THEN + CALL SQPF3(STRING,MATCH,IDIM2I,IDIM2, + + KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF +C CALL SQPF4(KBIN,KBOUT,J1,J2,IS,IE, +C +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C IF(IOK.NE.0) RETURN + CALL SQPF5(IDIM2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PR,MINP,IOK) +C IF(IOK.NE.0) RETURN + RETURN + END + SUBROUTINE SQPF6(SEQNCE,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,KSTART,J1,J2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IOK) + CHARACTER SEQNCE(IDIM1),STRING(IDIM2) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + IOK = 1 + CALL FILLI(ITOT,ITOTID,0) + CALL FILLI(ITOTEL,ITOTID,0) + CALL SQFIT7(SEQNCE,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,KSTART) + IF(ITOTP.GT.ITOTID)THEN + IOK = 2 + RETURN + END IF + IF(ITOTP.GT.0)THEN + XMIN=J1 + XMAX=J2 + YMIN=0. + YMAX=IDIM2 + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + DO 203 I=1,ITOTP + X=ITOTEL(I) + Y=ITOT(I) + CALL LINE(X,X,YMIN,Y,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +203 CONTINUE + END IF + CALL VT100M + IOK = 0 + END + SUBROUTINE SEPFIT(SEQNCE,IDIM1,SEQ2,IDIM3I,ITOT,ITOTEL,ITOTID, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART, + +IDEV,IDEVIN,FILE2,KBIN,KBOUT,MATRIX,IDM, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) +C AUTHOR: RODGER STADEN + INTEGER DIALOG + PARAMETER (MAXSTR = 50) + CHARACTER HELPF*(*) + CHARACTER SEQNCE(IDIM1),STRING(MAXSTR) + CHARACTER SEQ2(IDIM3I),FILE2*(*) + CHARACTER MATCH(MAXSTR) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID),MATRIX(IDM,IDM) + INTEGER ANSTY,SMIN,SMAX + CALL SHOWFU(KBOUT,'Find matches using a score matrix') + MINP = 0 + IDIM2 = 0 + IDIM2I = MAXSTR + IDIM3 = IDIM3I + IS = J1 + IE = J2 + ISS = 1 + ISE = 10 + CALL GETPAR(19,1,IOK,ANSTY, + +IPAR2,IPAR3,IPAR4, + +IPAR5,IPAR6,IPAR7,IPAR8,IPAR9) + IF(IOK.NE.0) RETURN +5 CONTINUE + IF(DIALOG.EQ.1) THEN + CALL SQPFD1(SEQ2,IDIM3I,IDIM3,ANSTY,IDEVIN,FILE2, + + KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF +10 CONTINUE + CALL SEPFD2(SEQ2,IDIM3,MATCH,STRING, + +J1,J2,KSTART,ISS,ISE,IS,IE,IDIM2I,IDIM2,MAXSTR,ANSTY,MINP, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,MATRIX,IDM, + +SMIN,SMAX,IOK) + IF(IOK.NE.0) RETURN + CALL SEPF6(SEQNCE,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,KSTART,J1,J2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,MATRIX,IDM, + +SMIN,SMAX,IOK) + IF(IOK.EQ.2)THEN + WRITE(KBOUT,1017)ITOTID +1017 FORMAT(/,' more than',I6,' matches.', + + ' try changing score or region'/) + GO TO 10 + END IF + IF(IOK.NE.0) RETURN + CALL SEPF7(SEQNCE,IDIM1,STRING,IDIM2,MATCH,ITOT,ITOTEL,ITOTID, + +ITOTP,MINP,KSTART,J1,J2,ISS,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + GO TO 10 + END + SUBROUTINE SEPFD2(SEQ2,IDIM3,MATCH,STRING, + +J1,J2,KSTART,ISS,ISE,IS,IE,IDIM2I,IDIM2,MAXSTR,ANSTY,MINP, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,MATRIX,IDM, + +SMIN,SMAX,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + CHARACTER STRING(MAXSTR) + CHARACTER SEQ2(IDIM3) + CHARACTER MATCH(MAXSTR) + INTEGER ANSC,ANSTY,MATRIX(IDM,IDM),SMIN,SMAX + IOK = 1 + CALL YESNO(ANSC,'Keep picture', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANSC.LT.0)RETURN + IF(ANSC.EQ.1)CALL CLEARG + IF(ANSTY.EQ.1) THEN + CALL SEPF2(SEQ2,IDIM3,STRING,IDIM2I,IDIM2,ISS,ISE, + + KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + IF(ANSTY.EQ.0) THEN + CALL SQPF3(STRING,MATCH,IDIM2I,IDIM2, + + KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF +C CALL SEPF4(KBIN,KBOUT,J1,J2,IS,IE, +C +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C IF(IOK.NE.0) RETURN + CALL SEPF5(IDIM2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,MINP,MATRIX,IDM, + +SMIN,SMAX,STRING,IOK) +C IF(IOK.NE.0) RETURN + RETURN + END + SUBROUTINE SEPF2(SEQ2,IDIM3,STRING,IDIM2I,IDIM2,I1,I2, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ2(IDIM3),STRING(IDIM2I) + IOK = 1 + MININ = 1 + MAXIN = IDIM3 + WRITE(KBOUT,1000) +1000 FORMAT(' Define string ends') + CALL GETINT(MININ,MAXIN,I1,'Start',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF(IVAL.NE.I1) THEN + I2 = IVAL + 10 + END IF + I1 = IVAL + MININ = I1 + 1 + MAXIN = I1 + IDIM2I - 1 + CALL GETINT(MININ,MAXIN,I2,'End',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + I2 = IVAL + IDIM2 =I2 - I1 + 1 + CALL SQCOPY(SEQ2(I1),STRING,IDIM2) + WRITE(KBOUT,1001)(STRING(K),K=1,IDIM2) +1001 FORMAT(' string=',50A1) + IOK = 0 + END + SUBROUTINE SEPF5(IDIM2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,MINP,MATRIX,IDM, + +SMIN,SMAX,STRING,IOK) + CHARACTER HELPF*(*) + CHARACTER STRING(IDIM2) + INTEGER MATRIX(IDM,IDM),SMIN,SMAX,VALUE + IOK = 1 + CALL GTSCR(STRING,IDIM2,MATRIX,IDM,SMIN,SMAX) + WRITE(KBOUT,1000)SMIN,SMAX +1000 FORMAT(' Minimum score=',I6,' Maximum score=',I6) + MININ = SMIN + MAXIN = SMAX + IF(MINP.EQ.0) MINP = SMAX + CALL GETINT(MININ,MAXIN,MINP,'Score',VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINP = VALUE + IOK = 0 + END + SUBROUTINE SEPF6(SEQNCE,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,KSTART,J1,J2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,MATRIX,IDM, + +SMIN,SMAX,IOK) + CHARACTER SEQNCE(IDIM1),STRING(IDIM2) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID),MATRIX(IDM,IDM) + INTEGER SMIN,SMAX + IOK = 1 + CALL FILLI(ITOT,ITOTID,0) + CALL FILLI(ITOTEL,ITOTID,0) + CALL SPFIT(SEQNCE,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,MATRIX,IDM,KSTART) + IF(ITOTP.GT.ITOTID)THEN + IOK = 2 + RETURN + END IF + IF(ITOTP.GT.0)THEN + XMIN = J1 + XMAX = J2 + YMIN = SMIN + YMAX = SMAX + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + DO 203 I=1,ITOTP + X=ITOTEL(I) + Y=ITOT(I) + CALL LINE(X,X,YMIN,Y,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +203 CONTINUE + END IF + CALL VT100M + IOK = 0 + END +C SQFITP + SUBROUTINE SQFITP(SEQ,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,KSTART) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + IDIF=(IE-IS+2)-IDIM2 + IPSEQ=IS-KSTART+1 + ITOTP=0 + DO 200 I=1,IDIF + NTOT=0 + IP=IPSEQ + DO 100 J=1,IDIM2 + IF(SEQ(IP).EQ.STRING(J))THEN + NTOT=NTOT+1 + ELSE IF (STRING(J).EQ.'-')THEN + NTOT=NTOT+1 + END IF + IP=IP+1 +100 CONTINUE + IF(NTOT.GE.MINP)THEN + ITOTP=ITOTP+1 + IF(ITOTP.GT.ITOTID)RETURN + ITOT(ITOTP)=NTOT + ITOTEL(ITOTP)=IP-IDIM2+KSTART-1 + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + END +C SQFIT7 + SUBROUTINE SQFIT7(SEQ,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID + 1,IS,IE,MINS,ITOTP,KSTART) +C AUTHOR: RODGER STADEN +C COMPARE POINTERS, NOT ACTUAL CHARACTERS + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + INTEGER CTONUM + EXTERNAL CTONUM + IDIF=(IE-IS+2)-IDIM2 + IPSEQ=IS-KSTART+1 + ITOTP=0 + DO 200 I=1,IDIF + NTOT = 0 + IP=IPSEQ + DO 100 J=1,IDIM2 + IF(STRING(J).EQ.'-')THEN + NTOT = NTOT + 1 + ELSE + K = CTONUM(STRING(J)) + IF(K.EQ.CTONUM(SEQ(IP)))NTOT = NTOT + 1 + END IF + IP=IP+1 +100 CONTINUE + IF(NTOT.GE.MINS)THEN + ITOTP=ITOTP+1 + IF(ITOTP.GT.ITOTID)RETURN + ITOT(ITOTP)=NTOT + ITOTEL(ITOTP)=IP-IDIM2+KSTART-1 + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + END diff --git a/src/staden/anals89.f b/src/staden/anals89.f new file mode 100644 index 0000000..d8805fb --- /dev/null +++ b/src/staden/anals89.f @@ -0,0 +1,10995 @@ +C ANALS89 SUBROUTINES FOR NIP +C AUTHOR: RODGER STADEN +C 11-8-92 Fixed bug in x version: use of gtstr and opratr='all' when +C using feature tables: xversion returns default, fortran returns +C lin=0: now check for both. +C 29-7-92 Added routines for finding repeated words +C 6-8-92 trna: previously did not allow for case in conserved base check +C 29-5-92 set max open reading frame to idim/3 in opentr +C 27-5-92 trnad made max intron length default = min intron length (was 0) +C 21-5-92 fixed bug in restriction enzyme search: if 4 cutter file was +C selected it always got listed! +C 16-4-92 fixed bug in find inverted repeats: the second position was out! +C 8-1-90 +C Changed all kstran to jstran +C Changed all mstran to jstran +C Replaced calls codid1 by trand1 and deleted codid1 +C Added clos(unit=ideve) to tran3,codimp +C Changed format statement in trpiro to produce embl ft output +C 26-3-90 TRNA changed it to allow intron lengths from 0 to N (previously +C by mistake, had to be M to N where M is non zero). +C 29-3-90 Minor change to CODTDK call to CODTBO was erroneously sent ANST +C not ANSTO. Now sent ANSTO. +C 2-4-90 Major change to codtdk: normalised data not written to disk! +C 3-5-90 Minor change to inverted repeat search for case when a pallindrome +C is found: stop the subtraction of 2 from position for listed +C results by making intx1=intx2 +C 9-7-90 Removed menu routine +C 21-8-90 Changed getpar for option 39 to use completely new routines +C Changed TRPIRP and TRPIR and their callers +C 5-11-90 Changed calls to rdwmt to include flag for show/not show title +C 7-11-90 Huge number of changes to replace radio by radion, including +C writing new routine gstrnd. This has increased the returned +C value of the choice flag by 1 and so is a potential source +C of minor cockup +C 21-12-90 Changed weight matrix searches so that text output gives position +C as middle + i (was i) +C 11-1-91 Greatly modified positional base preferences method adding +C facility to read codon table from disk. +C 19-2-91 Modified s2 to accomodate longer restriction enzyme recognition +C seqs, and moved a write statement from findl4, findl5 to s2. +C For output from s2 names are now limited to 15 chars +C recognition seqs to 35 chars. +C 22-2-91 trpir had forgotten to reverse translations from the complementary +C strand. Added call to sqrev. +C 11-4-91 modified trand7 to make 6 phase translation more obvious +C 18-4-91 Replaced splice search and changed setpar +C 23-4-91 Minor change to trand8 to allow both strands +C New routines: trnpir, trndop, trndom, openfr, trndp, trndm, +C wpair, pstop, mstop, minil. For translating open frames to disk +C and finding open frames +C 11-6-91 Following routines replaced or added.Changes relating to new fts +C 24-2-92 Bracketed the factor of -1 in MBPRIM +C 2-3-92 set FILNAM = ' ' for all openf1 calls +C SUBROUTINE OPENTR(SEQ,IDIM,KSTART,IDEV,KBIN,KBOUT,PAA, +C SUBROUTINE TRANEM(SEQ,IDIM,J1,J2,FRAMEC, +C SUBROUTINE TRAND8(IDIM,J1,J2,KSTRAN,IP1,IP2,PROMPT, +C SUBROUTINE TRAND7(KBIN,KBOUT,I1,I2, +C SUBROUTINE EMBLF(IDEVE,FILNAM, +C SUBROUTINE TRAND9(KBIN,KBOUT,IDEVE,FILNAM,OPRATR, +C SUBROUTINE TRANEN(SEQ,IDIM,J1,J2,FRAMEC,JSTRAN,IDEVE, +C SUBROUTINE TRAND1(JSTRAN,ANSE,IDEVE,FILNAM, +C SUBROUTINE TRANDK(SEQ1,IDIM1,OUTP1,IDIMP,ISTART,ISTOP,IDIMT, +C SUBROUTINE TRNEMB(SEQ,IDIM,J1,J2,SEQW,PAA,JSTRAN,IDEVE,IDEV, +C SUBROUTINE CODTDK(SEQ,IDIM,J1,J2,IDEVE,FILNAM,KBIN,KBOUT, +C SUBROUTINE CODND1(JSTRAN,NORM,ANSE,IDEVE,FILNAM,OPRATR, +C SUBROUTINE CTDEMB(SEQ,IDIM,J1,J2,SEQW,PAA,JSTRAN,IDEVE, +C SUBROUTINE CODEMB(SEQ,IDIM,J1,J2,SEQW,PAA,JSTRAN,IDEVE,IDEV, +C SUBROUTINE CODONS(SEQ,IDIM,J1,J2,IDEV,IDEVE,FILNAM,KBIN,KBOUT, +C SUBROUTINE CODIMP(SEQ,IDIM,FTABLE,IDFTAB,IDEVE,FILNAM, +C SUBROUTINE CDIEMB(SEQ,IDIM,J1,J2,SEQW,JSTRAN,IDEVE,IDEV, +C SUBROUTINE PLTEMB(JSTRAN,IDEVE, +C SUBROUTINE PLTMAQ(IDEV,FILNAM,POSNS,MAXPOS, +C SUBROUTINE PLTBAR(POSNL,POSNR,YF,YF,BLIPB,BLIPT, +C SUBROUTINE MBPRIM(FRAMEC,IDIMS,J1,J2,PRIME) +C 17-7-91 trndom replced line to write out title! which had disapeared + SUBROUTINE GETPAR(IOPT,NPAM,IOK, + +I1,I2,I3,I4,I5,I6,I7,I8,I9,I10, + +I11,I12,I13,I14,I15,I16,I17,I18,I19,I20, + +I21,I22,I23,I24,I25,I26,I27,I28,I29,I30, + +I31,I32,I33,I34,I35) + PARAMETER (MINOPT = 17, + + MAXOPT = 68, + + NUMBER = 35) + INTEGER VALUES(NUMBER,MINOPT:MAXOPT) + COMMON /PAMDEF/VALUES + IOK = 0 + IF((IOPT.GE.MINOPT).AND.(IOPT.LE.MAXOPT)) THEN + I = 1 + I1 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I2 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I3 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I4 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I5 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I6 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I7 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I8 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I9 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I10 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I11 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I12 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I13 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I14 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I15 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I16 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I17 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I18 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I19 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I20 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I21 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I22 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I23 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I24 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I25 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I26 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I27 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I28 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I29 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I30 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I31 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I32 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I33 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I34 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + I = I + 1 + I35 = VALUES(I,IOPT) + IF(I.EQ.NPAM) RETURN + END IF + IOK = 1 + END + SUBROUTINE SETPAR(IOK) + PARAMETER (MINOPT = 17, + + MAXOPT = 68, + + NUMBER = 35) + INTEGER VALUES(NUMBER,MINOPT:MAXOPT) +C INTEGER TEMP(NUMBER) + COMMON /PAMDEF/VALUES + SAVE /PAMDEF/ + IOK = 0 +C SEARCH +C CALL GETPAR(17,14,IOK, +C +MINLEN,MAXLEN,LINLEN,MNCUT,MAXCUT,MINCUT, +C +OPTION,OPTOUT,IFILE,JTRAN,ITRAN,IROUND,IDEF) + VALUES(1,17) = 10 + VALUES(2,17) = 100 + VALUES(3,17) = 60 + VALUES(4,17) = 0 + VALUES(5,17) = 100 + VALUES(6,17) = 0 + VALUES(7,17) = 0 + VALUES(8,17) = 0 + VALUES(9,17) = 2 + VALUES(10,17) = 0 + VALUES(11,17) = 0 + VALUES(12,17) = 0 + VALUES(13,17) = 0 +C PERCENT MATCH + VALUES(1,18) = 0 + VALUES(2,18) = 70 +C SCORE MATRIX MATCH + VALUES(1,19) = 0 +C WEIGHT MATRIX + VALUES(1,20) = 0 +C WRITE(KBOUT,*)' CALCULATE CODON USAGE, BASE COMPOSITION' +C WRITE(KBOUT,*)' AND AMINO ACID COMPOSITION' +C CALL GETPAR(23,3,IOK,JSTRAN,NORM,ANSE) + VALUES(1,23) = 0 + VALUES(2,23) = 0 + VALUES(3,23) = 0 +C +C PLOT BASE COMPOSITION +C +C CALL GETPAR(24,10,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, +C +SCORES(1),SCORES(2),SCORES(3),SCORES(4)) +C + VALUES(1,24) = 1 + VALUES(2,24) = 201 + VALUES(3,24) = 31 + VALUES(4,24) = 1 + VALUES(5,24) = 11 + VALUES(6,24) = 5 + VALUES(7,24) = 1 + VALUES(8,24) = 0 + VALUES(9,24) = 1 + VALUES(10,24) = 0 +C WRITE(KBOUT,*)' PLOT BASE COMPOSITION DIFFERENCES AS CHI SQUARED' +C CALL GETPAR(25,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,25) = 11 + VALUES(2,25) = 301 + VALUES(3,25) = 33 + VALUES(4,25) = 1 + VALUES(5,25) = 21 + VALUES(6,25) = 5 +C WRITE(KBOUT,*)' PLOT DI COMPOSITION DIFFERENCES AS CHI SQUARED' +C CALL GETPAR(26,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,26) = 11 + VALUES(2,26) = 301 + VALUES(3,26) = 33 + VALUES(4,26) = 1 + VALUES(5,26) = 21 + VALUES(6,26) = 5 +C WRITE(KBOUT,*)' PLOT TRI COMPOSITION DIFFERENCES AS CHI SQUARED' +C CALL GETPAR(27,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,27) = 11 + VALUES(2,27) = 301 + VALUES(3,27) = 33 + VALUES(4,27) = 1 + VALUES(5,27) = 21 + VALUES(6,27) = 5 +C WRITE(KBOUT,*)' CALCULATE CODON IMPROBABILITY' +C CALL GETPAR(28,2,IOK,JSTRAN,ANSE) + VALUES(1,28) = 0 + VALUES(2,28) = 0 +C WRITE(KBOUT,*)' SEARCH FOR HAIRPIN LOOPS' +C CALL GETPAR(30,10,IOK,MINLPI,MAXLPI,MINLP, +C +MINLPX,MAXLPX,MAXLP,MINBP,MAXBP,MININ,IGON) + VALUES(1,30) = 1 + VALUES(2,30) = 30 + VALUES(3,30) = 1 + VALUES(4,30) = 3 + VALUES(5,30) = 120 + VALUES(6,30) = 3 + VALUES(7,30) = 2 + VALUES(8,30) = 20 + VALUES(9,30) = 6 + VALUES(10,30) = 0 +C WRITE(KBOUT,*)' PLOT INVERTED REPEATS' +C CALL GETPAR(31,4,IOK,MINRP,MAXRP,MINREP,IGON) + VALUES(1,31) = 6 + VALUES(2,31) = 30 + VALUES(3,31) = 12 + VALUES(4,31) = 0 +C WRITE(KBOUT,*)' PLOT REPEATS' +C CALL GETPAR(32,4,IOK,MINRP,MAXRP,MINREP,IGON) + VALUES(1,32) = 6 + VALUES(2,32) = 30 + VALUES(3,32) = 12 + VALUES(4,32) = 0 +C WRITE(KBOUT,*)' PLOT Z DNA POTENTIAL ' +C CALL GETPAR(33,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,33) = 3 + VALUES(2,33) = 33 + VALUES(3,33) = 15 + VALUES(4,33) = 1 + VALUES(5,33) = 21 + VALUES(6,33) = 3 +C WRITE(KBOUT,*)' PLOT Z DNA POTENTIAL ' +C CALL GETPAR(34,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,34) = 3 + VALUES(2,34) = 33 + VALUES(3,34) = 21 + VALUES(4,34) = 1 + VALUES(5,34) = 11 + VALUES(6,34) = 1 +C WRITE(KBOUT,*)' PLOT Z DNA POTENTIAL ' +C CALL GETPAR(35,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,35) = 5 + VALUES(2,35) = 33 + VALUES(3,35) = 15 + VALUES(4,35) = 1 + VALUES(5,35) = 21 + VALUES(6,35) = 1 +C WRITE(KBOUT,*)' SEARCH FOR LOCAL SIMILARITY OR COMPLEMENTARITY' +C CALL GETPAR(36,15,IOK,MINSP,MAXSP,SPAN,MINPR,MAXPR,IPR, +C +MNIR1,MXIR1,IRAN1,MNIR2,MXIR2,IRAN2, +C +ANSRV,ANSC,IGON) + VALUES(1,36) = 5 + VALUES(2,36) = 200 + VALUES(3,36) = 15 + VALUES(4,36) = 5 + VALUES(5,36) = 100 + VALUES(6,36) = 70 + VALUES(7,36) = 1 + VALUES(8,36) = 50 + VALUES(9,36) = 1 + VALUES(10,36) = 1 + VALUES(11,36) = 50 + VALUES(12,36) = 1 + VALUES(13,36) = 0 + VALUES(14,36) = 0 + VALUES(15,36) = 0 +C WRITE(KBOUT,*)' LIST TRANSLATION (UP TO 3 PHASES)' +C CALL GETPAR(38,6,IOK,MINLEN,MAXLEN,LINLEN,JSTRAN,ANSE,ITRAN) + VALUES(1,38) = 30 + VALUES(2,38) = 120 + VALUES(3,38) = 60 + VALUES(4,38) = 0 + VALUES(5,38) = 0 + VALUES(6,38) = 0 +C WRITE(KBOUT,*)' TRANSLATE IN SIX PHASES' +C CALL GETPAR(39,11,IOK,MINLEN,MAXLEN,LINLEN,ITRAN, +C CALL GETPAR(39,10,IOK,MINO,MINOPN,JSTRAN, +C MINLEN,MAXLEN,LINLEN,ITRAN,JTRAN,INUM,ANSE, + VALUES(1,39) = 0 + VALUES(2,39) = 30 + VALUES(3,39) = 0 + VALUES(4,39) = 30 + VALUES(5,39) = 120 + VALUES(6,39) = 60 + VALUES(7,39) = 0 + VALUES(8,39) = 0 + VALUES(9,39) = 0 + VALUES(10,39) = 3 +C WRITE(KBOUT,*)' TRANSLATION AND WRITE PROTEIN SEQUENCE TO DISK' +C CALL GETPAR(40,7,IOK,JSTRAN,ANSE,IOPEN,MINO,MAXO,MINOPN,JSTRAN) + VALUES(1,40) = 0 + VALUES(2,40) = 0 + VALUES(3,40) = 0 + VALUES(4,40) = 5 + VALUES(5,40) = 1000 + VALUES(6,40) = 30 + VALUES(7,40) = 0 +C WRITE(KBOUT,*)' CALCULATE AND WRITE CODON TABLE TO DISK' +C CALL GETPAR(41,5,IOK,JSTRAN,NORM,ANSE,ANST,ANSTO) + VALUES(1,41) = 0 + VALUES(2,41) = 0 + VALUES(3,41) = 0 + VALUES(4,41) = 0 + VALUES(5,41) = 0 +C WRITE(KBOUT,*)'STADEN AND MCLACHLAN CODON USAGE METHOD' +C CALL GETPAR(42,7,IOK,MINSP,MAXSP,LENW,MINIW,MAXIW,IWRIT,ANSTY) + VALUES(1,42) = 11 + VALUES(2,42) = 101 + VALUES(3,42) = 25 + VALUES(4,42) = 1 + VALUES(5,42) = 11 + VALUES(6,42) = 5 + VALUES(7,42) = 0 +C WRITE(KBOUT,*)' POSITIONAL BASE PREFERENCES METHOD TO FIND', +C +' PROTEIN GENES' +C CALL GETPAR(43,9,IOK,MINSP,MAXSP,LENW,MINIW,MAXIW,IWRIT, +C +ANSABS,ANSTY,ANSS) + VALUES(1,43) = 31 + VALUES(2,43) = 101 + VALUES(3,43) = 67 + VALUES(4,43) = 1 + VALUES(5,43) = 11 + VALUES(6,43) = 5 + VALUES(7,43) = 0 + VALUES(8,43) = 0 + VALUES(9,43) = 0 +C WRITE(KBOUT,*)' UNEVEN POSITIONAL BASE FREQUENCIES METHOD' +C CALL GETPAR(44,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,44) = 11 + VALUES(2,44) = 101 + VALUES(3,44) = 67 + VALUES(4,44) = 1 + VALUES(5,44) = 21 + VALUES(6,44) = 5 +C WRITE(KBOUT,*)' SEARCH FOR PROTEIN GENES USING MCLACHLAN, STADEN' +C WRITE(KBOUT,*)' AND BOSWELL IMPROBABILITY METHOD' +C WRITE(KBOUT,*)' EXPECTING CODON COMPOSITION TO DEPEND ON' +C WRITE(KBOUT,*)' BASE COMPOSITION' +C CALL GETPAR(45,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,45) = 67 + VALUES(2,45) = 99 + VALUES(3,45) = 99 + VALUES(4,45) = 1 + VALUES(5,45) = 21 + VALUES(6,45) = 5 +C WRITE(KBOUT,*)' SEARCH FOR PROTEIN GENES USING MCLACHLAN, STADEN' +C WRITE(KBOUT,*)' AND BOSWELL IMPROBABILITY METHOD' +C WRITE(KBOUT,*)' EXPECTING CODON COMPOSITION TO DEPEND ON' +C WRITE(KBOUT,*)' AMINO ACID COMPOSITION' +C CALL GETPAR(46,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,46) = 67 + VALUES(2,46) = 99 + VALUES(3,46) = 99 + VALUES(4,46) = 1 + VALUES(5,46) = 21 + VALUES(6,46) = 5 +C WRITE(KBOUT,*)' SHEPHERDS METHOD' +C CALL GETPAR(47,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,47) = 11 + VALUES(2,47) = 101 + VALUES(3,47) = 67 + VALUES(4,47) = 1 + VALUES(5,47) = 21 + VALUES(6,47) = 5 +C WRITE(KBOUT,*)' FICKETTS METHOD' +C CALL GETPAR(48,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,48) = 11 + VALUES(2,48) = 101 + VALUES(3,48) = 67 + VALUES(4,48) = 1 + VALUES(5,48) = 21 + VALUES(6,48) = 5 +C WRITE(KBOUT,*)' TRNA SEARCH ' +C CALL GETPAR(49,35,IOK, +C +MNLEN,MXLEN,MAXLNT, +C +MNAC,MXAC,MINACL, +C +MNTU,MXTU,MINTU, +C +MNAN,MXAN,MINANT, +C +MND,MXD,MIND, +C +MNI1,MXI1,INT1, +C +MNI2,MXI2,INT2, +C +MNTUL1,MXTUL1,LTUMIN, +C +MNTUL2,MXTUL2,LTUMAX, +C +MNB,MXB,MINBAS, +C +MNC,MXC,MINCON,IGON,ANSCON) + VALUES(1,49) = 70 + VALUES(2,49) = 130 + VALUES(3,49) = 92 + VALUES(4,49) = 0 + VALUES(5,49) = 14 + VALUES(6,49) = 11 + VALUES(7,49) = 0 + VALUES(8,49) = 10 + VALUES(9,49) = 8 + VALUES(10,49) = 0 + VALUES(11,49) = 10 + VALUES(12,49) = 8 + VALUES(13,49) = 0 + VALUES(14,49) = 8 + VALUES(15,49) = 3 + VALUES(16,49) = 0 + VALUES(17,49) = 30 + VALUES(18,49) = 0 + VALUES(19,49) = 30 + VALUES(20,49) = 30 + VALUES(21,49) = 0 + VALUES(22,49) = 4 + VALUES(23,49) = 12 + VALUES(24,49) = 6 + VALUES(25,49) = 6 + VALUES(26,49) = 12 + VALUES(27,49) = 9 + VALUES(28,49) = 0 + VALUES(29,49) = 44 + VALUES(30,49) = 0 + VALUES(31,49) = 0 + VALUES(32,49) = 0 + VALUES(33,49) = 0 + VALUES(34,49) = 0 + VALUES(35,49) = 1 +C WRITE(KBOUT,*)' FIND OPEN READING FRAMES' +C CALL GETPAR(54,4,IOK,MINO,MAXO,MINOPN,JSTRAN) + VALUES(1,54) = 5 + VALUES(2,54) = 1000 + VALUES(3,54) = 30 + VALUES(4,54) = 0 +C WRITE(KBOUT,*)' PLOT NEGENTROPY' +C CALL GETPAR(29,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,59) = 11 + VALUES(2,59) = 301 + VALUES(3,59) = 33 + VALUES(4,59) = 1 + VALUES(5,59) = 21 + VALUES(6,59) = 5 +C SPLICE SEARCH + VALUES(1,62) = 0 +C WRITE(KBOUT,*)'PLOT COMPOSITION DIFFERENCES (OBS-EXP))' +C CALL GETPAR(64,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT) + VALUES(1,64) = 3 + VALUES(2,64) = 401 + VALUES(3,64) = 101 + VALUES(4,64) = 1 + VALUES(5,64) = 20 + VALUES(6,64) = 5 +C PATTERNS TO DO +C OPEN(UNIT=99,FILE='ANALYSEP.PAR',STATUS ='OLD') +C10 CONTINUE +C READ(99,*,ERR=100,END=200)TEMP +C IF((TEMP(1).GE.MINOPT).AND.(TEMP(1).LE.MAXOPT)) THEN +C DO 20 I = 1,NUMBER-1 +C VALUES(I,TEMP(1)) = TEMP(I+1) +C WRITE(*,*)TEMP(1),TEMP(I+1) +C20 CONTINUE +C END IF +C GO TO 10 +C100 CONTINUE +C WRITE(*,*)'ERROR IN PARMS FILE' +C GO TO 10 +C200 CONTINUE +C CLOSE(UNIT = 99) + IOK = 0 + END + SUBROUTINE SEARCH(SEQ,IDSEQ,J1,J2,KSTART, + +RENZYM,MAXREN,RECSEQ,MAXREC, + +NAMES,MAXNAM,CUTADD,PSAVE,NSAVE,SORTA,MAXMAT, + +NAMEP,NAMLEN,NUMREC,LENEN,RECSTR,NAMLST, + +CUTSIT,PCUT,MAXENZ, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV, + +IDEVEN,FILNAM,FILE4,FILE6,FILEA,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PAA,POSN,WORDP,MAXDIC,DIALOG) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),FILEA*(*),FILE4*(*),FILE6*(*),HELPF*(*) + CHARACTER SEQ(IDSEQ),RENZYM(MAXREN),RECSEQ(MAXREC),NAMES(MAXNAM) + CHARACTER PAA(125) + INTEGER OPTION,OPTOUT + INTEGER CUTSIT(MAXENZ),PCUT(MAXENZ) + INTEGER CUTADD(MAXMAT),PSAVE(MAXMAT),NSAVE(MAXMAT),SORTA(MAXMAT) + INTEGER NAMEP(MAXENZ),NAMLEN(MAXENZ),NUMREC(MAXENZ) + INTEGER LENEN(MAXENZ) + INTEGER RECSTR(MAXENZ),NAMLST(MAXENZ),DIALOG + INTEGER POSN(IDSEQ),WORDP(MAXDIC),CONST(0:24) + PARAMETER (IBH=256) + CALL SHOWFU(KBOUT,'Search for restriction enzyme sites') + CALL GETPAR(17,13,IOK, + +MINLEN,MAXLEN,LINLEN,MNCUT,MAXCUT,MINCUT, + +OPTION,OPTOUT,IFILE,JTRAN,KTRAN,IROUND,IDEF, + +IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + INC = 1 + IDNDEX = 1024 + LENW = 5 + LENC = 4*LENW + CALL SETCN(CONST,LENW,5,LENC) + ITRAN = KTRAN +10 CONTINUE + IDREN = MAXREN + IDRSEQ = MAXREC + IDNAML = MAXNAM + IDNLST = MAXENZ + MAXEN = MAXENZ + IDMAT = MAXMAT + LEVEL = 0 + CALL SERCHD(RENZYM,IDREN,RECSEQ,IDRSEQ, + +NAMES,IDNAML,MARGT, + +NAMEP,NAMLEN,NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST, + +IDEVEN,FILNAM,FILE4,FILE6,FILEA,KBIN,KBOUT, + +OPTION,OPTOUT,NOPOUT,IFILE,DIALOG, + +IHELPS,IHELPE,HELPF,IDEVH,IOK,CUTSIT,PCUT,MAXS,LINLEN,ITRAN, + +JTRAN,MINLEN,MAXLEN,IROUND,IDEF,MINCUT,MNCUT,MAXCUT) + IF(IOK.NE.0) RETURN + CALL BUSY(KBOUT) + CALL CONNUM(SEQ(J1),POSN(J1),J2-J1+1) + CALL ENCOND(POSN(J1),J2-J1+1,WORDP,IDNDEX,5,CONST,LENW,LENC) + IF(OPTOUT.EQ.2) THEN + CALL FINDL1(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ,NAMES,IDNAML, + + NAMEP,NAMLEN, + + NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PCUT,CUTSIT,MAXS,IROUND, + + PSAVE,NSAVE,CUTADD,SORTA,IDMAT,NOPOUT,IBH,LEVEL, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + + IHELPS,IHELPE,HELPF,IDEVH,MINCUT,IDEF, + + POSN(J1),WORDP,IDNDEX,CONST,LENW,LENC,IOK) + ELSE IF((OPTOUT.EQ.0).AND.(NOPOUT.EQ.1)) THEN + CALL FINDL2(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ,NAMES,IDNAML, + + NAMEP,NAMLEN, + + NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PCUT,CUTSIT,MAXS,IROUND, + + PSAVE,NSAVE,CUTADD,SORTA,IDMAT,NOPOUT,IBH,LEVEL, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + + IHELPS,IHELPE,HELPF,IDEVH,IDEF, + + POSN(J1),WORDP,IDNDEX,CONST,LENW,LENC,IOK) + ELSE IF((OPTOUT.EQ.1).AND.(NOPOUT.EQ.1)) THEN + CALL FINDL3(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ,NAMES,IDNAML, + + NAMEP,NAMLEN, + + NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PCUT,CUTSIT,MAXS,IROUND, + + PSAVE,NSAVE,CUTADD,SORTA,IDMAT,NOPOUT,IBH,LEVEL, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + + IHELPS,IHELPE,HELPF,IDEVH,IDEF, + + POSN(J1),WORDP,IDNDEX,CONST,LENW,LENC,IOK) + ELSE IF((OPTOUT.EQ.0).AND.(NOPOUT.EQ.0)) THEN + CALL FINDL4(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ,NAMES,IDNAML, + + NAMEP,NAMLEN, + + NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PCUT,CUTSIT,MAXS,IROUND, + + PSAVE,NSAVE,CUTADD,SORTA,IDMAT,NOPOUT,IBH,LEVEL, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + + IHELPS,IHELPE,HELPF,IDEVH,IDEF, + + POSN(J1),WORDP,IDNDEX,CONST,LENW,LENC,IOK) + ELSE IF((OPTOUT.EQ.1).AND.(NOPOUT.EQ.0)) THEN + CALL FINDL5(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ,NAMES,IDNAML, + + NAMEP,NAMLEN, + + NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PCUT,CUTSIT,MAXS,IROUND, + + PSAVE,NSAVE,CUTADD,SORTA,IDMAT,NOPOUT,IBH,LEVEL, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + + IHELPS,IHELPE,HELPF,IDEVH,IDEF, + + POSN(J1),WORDP,IDNDEX,CONST,LENW,LENC,IOK) + ELSE IF((OPTOUT.EQ.3).AND.(NOPOUT.EQ.0)) THEN + CALL FINDL6(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ,NAMES,IDNAML, + + NAMEP,NAMLEN, + + NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PCUT,CUTSIT,MAXS,IROUND, + + PSAVE,NSAVE,CUTADD,SORTA,IDMAT,NOPOUT,IBH,LEVEL, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + + IHELPS,IHELPE,HELPF,IDEVH,ITRAN,LINLEN,PAA,IDEF, + + POSN(J1),WORDP,IDNDEX,CONST,LENW,LENC,IOK) + END IF + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + DIALOG = 1 + GO TO 10 + END + SUBROUTINE SERCHD(RENZYM,IDREN,RECSEQ,IDRSEQ, + +NAMES,IDNAML,MARGT, + +NAMEP,NAMLEN,NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST, + +IDEVEN,FILNAM,FILE4,FILE6,FILEA,KBIN,KBOUT, + +OPTION,OPTOUT,NOPOUT,IFILE,DIALOG, + +IHELPS,IHELPE,HELPF,IDEVH,IOK,CUTSIT,PCUT,MAXS,LINLEN,ITRAN, + +JTRAN,MINLEN,MAXLEN,IROUND,IDEF,MINCUT,MNCUT,MAXCUT) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),FILEA*(*),FILE4*(*),FILE6*(*),HELPF*(*) + CHARACTER RENZYM(IDREN),RECSEQ(IDRSEQ),NAMES(IDNAML) + INTEGER OPTION,OPTOUT + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN) + INTEGER LENEN(MAXEN) + INTEGER RECSTR(MAXEN),NAMLST(IDNLST),DIALOG + INTEGER CUTSIT(IDNLST),PCUT(IDNLST) + PARAMETER (MAXPRM = 30) + CHARACTER PROMPT(4)*(MAXPRM) + IOK = 0 + NOPOUT = 0 + CALL GETREN(RENZYM,IDREN,IDEVEN,FILNAM,FILE4,FILE6,FILEA, + +IOK,KBIN,KBOUT,IFILE,DIALOG, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + IF(IDREN.LT.5) THEN + IOK = 1 + RETURN + END IF + CALL DECREN(RENZYM,IDREN,RECSEQ,IDRSEQ,NAMES,IDNAML, + +NAMEP,NAMLEN,NUMREC,LENEN,RECSTR,MAXEN,KBOUT,CUTSIT,PCUT,MAXS) + IF(MAXEN.LT.1) THEN + IOK = 1 + RETURN + END IF + IF(DIALOG.EQ.0) RETURN + IOP = OPTION + CALL YESONO(IOP,'Search for all names','select names', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) THEN + IOK = 1 + RETURN + END IF + OPTION = IOP + IF(OPTION.EQ.1)THEN + CALL GETNAM(NAMLST,IDNLST,NAMES,IDNAML,NAMEP,NAMLEN, + + MAXEN,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IDNLST.LT.1) THEN + IOK = 1 + RETURN + END IF + END IF + IOP = OPTOUT + 1 + PROMPT(1) = 'Order results enzyme by enzyme' + PROMPT(2) = 'Order results by position' + PROMPT(3) = 'Show only infrequent cutters' + PROMPT(4) = 'Show names above the sequence' + CALL RADION('Select results display mode',PROMPT,4,IOP, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.1) THEN + IOK = 1 + RETURN + END IF + OPTOUT = IOP - 1 + IF(OPTOUT.EQ.2) THEN + CALL GETINT(MNCUT,MAXCUT,MINCUT,'Maximum number of cuts', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINCUT = IVAL + END IF + IF(OPTOUT.EQ.3) THEN + IOP = JTRAN + CALL YESONO(IOP,'Hide translation','Show translation', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) THEN + IOK = 1 + RETURN + END IF + JTRAN = IOP + IF(JTRAN.EQ.1) THEN + IOP = ITRAN + IF(IOP.EQ.3) IOP = 1 + CALL YESONO(IOP,'Use 1 letter codes', + + 'Use 3 letter codes', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) THEN + IOK = 1 + RETURN + END IF + IF(IOP.EQ.1) ITRAN = 3 + IF(IOP.EQ.0) ITRAN = 0 + ELSE IF(JTRAN.EQ.0) THEN + ITRAN = 1 + END IF + IF(ITRAN.NE.1) THEN + MAXLEN = 90 + MINLEN = 30 + END IF + LENDEF = LINLEN + CALL GETINT(MINLEN,MAXLEN,LENDEF,'Line length', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + LINLEN = IVAL + IF((ITRAN.NE.1).AND.(MOD(LINLEN,3).NE.0)) LINLEN = 60 + END IF + IOP = NOPOUT + IF((OPTOUT.EQ.0).OR.(OPTOUT.EQ.1)) THEN + CALL YESONO(IOP,'List matches','Plot matches', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) THEN + IOK = 1 + RETURN + END IF + NOPOUT = IOP + END IF +C IF(NOPOUT.EQ.1)THEN +C LEVEL = LEVELI +C CALL SD2(IBLIPH,IBH,LEVEL,MARGT, +C + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) +C LEVELI = LEVEL +C IF(IOK.NE.0) RETURN +C END IF +C CALL GETINT(MININC,MAXINC,INC,'Step', +C +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) +C IF(IOK.NE.0) RETURN +C INC = IVAL + IOP = IROUND + CALL YESONO(IOP,'The sequence is linear', + +'The sequence is circular', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) THEN + IOK = 1 + RETURN + END IF + IROUND = IOP + IOP = IDEF + CALL YESONO(IOP,'Search for definite matches', + +'Search for possible matches', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) THEN + IOK = 1 + RETURN + END IF + IDEF = IOP + END + SUBROUTINE FINDL1(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ, + +NAMES,IDNAML,NAMEP,NAMLEN, + +NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PCUT,CUTSIT,MAXS,IROUND, + +PSAVE,NSAVE,CUTADD,SORTA,MAXMAT,NOPOUT,IBH,LEVEL, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,MINCUT,IDEF, + +POSN,WORDP,IDNDEX, + +CONST,LENW,LENC,IOK) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),NAMES(IDNAML),RECSEQ(IDRSEQ) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN),LENEN(MAXS) + INTEGER RECSTR(MAXEN),NAMLST(IDNLST),PSAVE(MAXMAT) + INTEGER NSAVE(MAXMAT),OPTION,OPTOUT + INTEGER CUTSIT(MAXS),PCUT(MAXEN),CUTADD(MAXMAT),SORTA(MAXMAT) + CHARACTER HELPF*(*) + INTEGER POSN(IDSEQ),WORDP(IDNDEX),CONST(0:LENC) +C OPTOUT=0 ENZYME BY ENZYME +C LOW FREQUENCY CUTTERS +C names max length 20 chars, strings 20 chars + LAST=MAXEN + IF(OPTION.EQ.1)LAST=IDNLST + DO 100 IEN=1,LAST + IFOUND=0 + JEN=IEN + IF(OPTION.EQ.1)JEN=NAMLST(IEN) + NREC=NUMREC(JEN) + IF(IDEF.EQ.0) THEN + CALL S1D0(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + + PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND, + + POSN,WORDP,IDNDEX, + + CONST,LENW,LENC,KBOUT,IOK) + ELSE + CALL S1D1(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + + PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND,KBOUT,IOK) + END IF + IF(IOK.NE.0) RETURN + IF(IFOUND.LE.MINCUT) THEN + K1=NAMEP(JEN) + K2=K1+NAMLEN(JEN)-1 + WRITE(IDEV,1009)IFOUND,(NAMES(K),K=K1,K2) +1009 FORMAT(' ',I6,' ',20A1) + END IF +100 CONTINUE + END + SUBROUTINE FINDL2(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ, + +NAMES,IDNAML,NAMEP,NAMLEN, + +NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PCUT,CUTSIT,MAXS,IROUND, + +PSAVE,NSAVE,CUTADD,SORTA,MAXMAT,NOPOUT,IBH,LEVEL, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,IDEF, + +POSN,WORDP,IDNDEX, + +CONST,LENW,LENC,IOK) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),NAMES(IDNAML),RECSEQ(IDRSEQ) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN),LENEN(MAXS) + INTEGER RECSTR(MAXEN),NAMLST(IDNLST),PSAVE(MAXMAT) + INTEGER NSAVE(MAXMAT),OPTION,OPTOUT + INTEGER CUTSIT(MAXS),PCUT(MAXEN),CUTADD(MAXMAT),SORTA(MAXMAT) + CHARACTER HELPF*(*) + CHARACTER*20 ATOS + INTEGER POSN(IDSEQ),WORDP(IDNDEX),CONST(0:LENC) + EXTERNAL ATOS +C OPTOUT=0 ENZYME BY ENZYME +C NOPOUT=1 GRAPHICAL OUTPUT +C FOR GRAPHICS NEED TO CHECK FOR END OF SCREEN + CALL CLEARV + CALL VECTOM + LEVEL1=LEVEL + LEVELM=MARGT-IBH +C names max length 20 chars, strings 20 chars + LAST=MAXEN + IF(OPTION.EQ.1)LAST=IDNLST + DO 100 IEN=1,LAST + IFOUND=0 + JEN=IEN + IF(OPTION.EQ.1)JEN=NAMLST(IEN) + NREC=NUMREC(JEN) + IF(IDEF.EQ.0) THEN + CALL S1D0(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + + PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND, + + POSN,WORDP,IDNDEX, + + CONST,LENW,LENC,KBOUT,IOK) + ELSE + CALL S1D1(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + + PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND,KBOUT,IOK) + END IF + IF(IOK.NE.0) THEN + CALL VT100M + RETURN + END IF + IF(IFOUND.GT.0) THEN + IF(LEVEL.GE.LEVELM)THEN + CALL PSRCHX(LEVEL1,LEVEL,KBIN,KBOUT,IQUIT, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IQUIT.EQ.1) THEN + CALL VT100M + RETURN + END IF + END IF + CALL PLSRCH(J1-KSTART+1,J2-KSTART+1, + + PSAVE,IFOUND,IBH,LEVEL, + + ATOS(NAMES(NAMEP(JEN)),NAMLEN(JEN)), + + NAMLEN(JEN), + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF +100 CONTINUE + CALL VT100M + END + SUBROUTINE FINDL3(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ, + +NAMES,IDNAML,NAMEP,NAMLEN, + +NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PCUT,CUTSIT,MAXS,IROUND, + +PSAVE,NSAVE,CUTADD,SORTA,MAXMAT,NOPOUT,IBH,LEVEL, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,IDEF, + +POSN,WORDP,IDNDEX, + +CONST,LENW,LENC,IOK) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),NAMES(IDNAML),RECSEQ(IDRSEQ) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN),LENEN(MAXS) + INTEGER RECSTR(MAXEN),NAMLST(IDNLST),PSAVE(MAXMAT) + INTEGER NSAVE(MAXMAT),OPTION,OPTOUT + INTEGER CUTSIT(MAXS),PCUT(MAXEN),CUTADD(MAXMAT),SORTA(MAXMAT) + CHARACTER HELPF*(*) + INTEGER POSN(IDSEQ),WORDP(IDNDEX),CONST(0:LENC) +C OPTOUT=1 ALL ENZYMES TOGETHER +C NOPOUT=1 GRAPHICAL OUTPUT +C FOR GRAPHICS NEED TO CHECK FOR END OF SCREEN + CALL CLEARV + CALL VECTOM + LEVEL1=LEVEL + LEVELM=MARGT-IBH + LAST=MAXEN + IF(OPTION.EQ.1)LAST=IDNLST + IFOUND=0 + DO 100 IEN=1,LAST + JEN=IEN + IF(OPTION.EQ.1)JEN=NAMLST(IEN) + NREC=NUMREC(JEN) + IF(IDEF.EQ.0) THEN + CALL S1D0(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + + PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND, + + POSN,WORDP,IDNDEX, + + CONST,LENW,LENC,KBOUT,IOK) + ELSE + CALL S1D1(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + + PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND,KBOUT,IOK) + END IF + IF(IOK.NE.0) THEN + CALL VT100M + RETURN + END IF +100 CONTINUE + IF(IFOUND.GT.0)THEN + CALL PLSRCH(J1-KSTART+1,J2-KSTART+1, + + PSAVE,IFOUND,IBH,LEVEL,'ALL',3, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + CALL VT100M + IF(IFOUND.EQ.0)WRITE(IDEV,3000) +3000 FORMAT(' No matches found') + END + SUBROUTINE FINDL4(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ, + +NAMES,IDNAML,NAMEP,NAMLEN, + +NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PCUT,CUTSIT,MAXS,IROUND, + +PSAVE,NSAVE,CUTADD,SORTA,MAXMAT,NOPOUT,IBH,LEVEL, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,IDEF, + +POSN,WORDP,IDNDEX, + +CONST,LENW,LENC,IOK) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),NAMES(IDNAML),RECSEQ(IDRSEQ) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN),LENEN(MAXS) + INTEGER RECSTR(MAXEN),NAMLST(IDNLST),PSAVE(MAXMAT) + INTEGER NSAVE(MAXMAT),OPTION,OPTOUT + INTEGER CUTSIT(MAXS),PCUT(MAXEN),CUTADD(MAXMAT),SORTA(MAXMAT) + CHARACTER HELPF*(*) + INTEGER POSN(IDSEQ),WORDP(IDNDEX),CONST(0:LENC) +C OPTOUT=0 ENZYME BY ENZYME +C names max length 20 chars, strings 20 chars + LAST=MAXEN + IF(OPTION.EQ.1)LAST=IDNLST + DO 100 IEN=1,LAST + IFOUND=0 + JEN=IEN + IF(OPTION.EQ.1)JEN=NAMLST(IEN) + NREC=NUMREC(JEN) + IF(IDEF.EQ.0) THEN + CALL S1D0(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + + PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND, + + POSN,WORDP,IDNDEX, + + CONST,LENW,LENC,KBOUT,IOK) + ELSE + CALL S1D1(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + + PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND,KBOUT,IOK) + END IF + IF(IOK.NE.0) RETURN + IF(IFOUND.GT.0) THEN + WRITE(IDEV,1010)IFOUND +1010 FORMAT(' Matches found=',I6) + CALL BUB3AS(PSAVE,NSAVE,CUTADD,IFOUND) +C calc lengths + IF(IROUND.EQ.0) THEN + LENN = J1 - KSTART + 1 + SORTA(1) = PSAVE(1) - LENN + ELSE IF(IROUND.EQ.1) THEN + LENN = J2 - PSAVE(IFOUND) + 1 + SORTA(1) = LENN + PSAVE(1) - (J1 - KSTART + 1) + LENN = 1 - LENN + END IF + DO 45 KOUT=2,IFOUND + SORTA(KOUT)=PSAVE(KOUT)-PSAVE(KOUT-1) +45 CONTINUE +C do length to end + IFP1 = IFOUND + IF(IROUND.EQ.0) THEN + SORTA(IFOUND+1)=J2-KSTART+1-PSAVE(IFOUND)+1 + IFP1=IFOUND+1 + END IF + CALL BUBBLE(SORTA,IFP1) + CALL S2(SEQ,IDSEQ,IFOUND,NAMEP,NAMLEN,LENEN,MAXEN, + + NSAVE,PSAVE,SORTA,MAXMAT,NAMES,IDNAML, + + CUTSIT,MAXS,CUTADD,IROUND, + + LENN,IDEV,J2,KSTART,0) + END IF +100 CONTINUE + END + SUBROUTINE FINDL5(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ, + +NAMES,IDNAML,NAMEP,NAMLEN, + +NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PCUT,CUTSIT,MAXS,IROUND, + +PSAVE,NSAVE,CUTADD,SORTA,MAXMAT,NOPOUT,IBH,LEVEL, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,IDEF, + +POSN,WORDP,IDNDEX, + +CONST,LENW,LENC,IOK) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),NAMES(IDNAML),RECSEQ(IDRSEQ) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN),LENEN(MAXS) + INTEGER RECSTR(MAXEN),NAMLST(IDNLST),PSAVE(MAXMAT) + INTEGER NSAVE(MAXMAT),OPTION,OPTOUT + INTEGER CUTSIT(MAXS),PCUT(MAXEN),CUTADD(MAXMAT),SORTA(MAXMAT) + CHARACTER HELPF*(*) + INTEGER POSN(IDSEQ),WORDP(IDNDEX),CONST(0:LENC) +C OPTOUT=1 ALL ENZYMES TOGETHER +C names max length 20 chars, strings 20 chars + LAST=MAXEN + IF(OPTION.EQ.1)LAST=IDNLST + IFOUND=0 + DO 100 IEN=1,LAST + JEN=IEN + IF(OPTION.EQ.1)JEN=NAMLST(IEN) + NREC=NUMREC(JEN) + IF(IDEF.EQ.0) THEN + CALL S1D0(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + + PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND, + + POSN,WORDP,IDNDEX, + + CONST,LENW,LENC,KBOUT,IOK) + ELSE + CALL S1D1(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + + PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND,KBOUT,IOK) + END IF + IF(IOK.NE.0) RETURN +100 CONTINUE + IF(IFOUND.GT.0) THEN + CALL BUB3AS(PSAVE,NSAVE,CUTADD,IFOUND) + IF(IROUND.EQ.0) THEN + LENN = J1 - KSTART + 1 + SORTA(1) = PSAVE(1) - LENN + ELSE IF(IROUND.EQ.1) THEN + LENN = J2 - PSAVE(IFOUND) + 1 + SORTA(1) = LENN + PSAVE(1) - (J1 - KSTART + 1) + LENN = 1 - LENN + END IF + DO 45 KOUT=2,IFOUND + SORTA(KOUT)=PSAVE(KOUT)-PSAVE(KOUT-1) +45 CONTINUE + CALL S2(SEQ,IDSEQ,IFOUND,NAMEP,NAMLEN,LENEN,MAXEN, + + NSAVE,PSAVE,SORTA,MAXMAT,NAMES,IDNAML, + + CUTSIT,MAXS,CUTADD,IROUND, + + LENN,IDEV,J2,KSTART,1) + ELSE + WRITE(IDEV,1010) +1010 FORMAT(' No matches found') + END IF + END + SUBROUTINE FINDL6(SEQ,IDSEQ,J1,J2,KSTART,RECSEQ,IDRSEQ, + +NAMES,IDNAML,NAMEP,NAMLEN, + +NUMREC,LENEN,RECSTR,MAXEN,NAMLST,IDNLST,OPTION,OPTOUT, + + PCUT,CUTSIT,MAXS,IROUND, + +PSAVE,NSAVE,CUTADD,SORTA,MAXMAT,NOPOUT,IBH,LEVEL, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,INC,KBIN,KBOUT,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,ITRAN,LINLEN,PAA,IDEF, + +POSN,WORDP,IDNDEX, + +CONST,LENW,LENC,IOK) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),NAMES(IDNAML),RECSEQ(IDRSEQ) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN),LENEN(MAXS) + INTEGER RECSTR(MAXEN),NAMLST(IDNLST),PSAVE(MAXMAT) + INTEGER NSAVE(MAXMAT),OPTION,OPTOUT + INTEGER CUTSIT(MAXS),PCUT(MAXEN),CUTADD(MAXMAT),SORTA(MAXMAT) + CHARACTER HELPF*(*),PAA(125) + INTEGER POSN(IDSEQ),WORDP(IDNDEX),CONST(0:LENC) +C OPTOUT=3 NAMES ABOVE SEQUENCE +C names max length 20 chars, strings 20 chars + LAST=MAXEN + IF(OPTION.EQ.1)LAST=IDNLST + IFOUND=0 + DO 100 IEN=1,LAST + JEN=IEN + IF(OPTION.EQ.1)JEN=NAMLST(IEN) + NREC=NUMREC(JEN) + IF(IDEF.EQ.0) THEN + CALL S1D0(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + + PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND, + + POSN,WORDP,IDNDEX, + + CONST,LENW,LENC,KBOUT,IOK) + ELSE + CALL S1D1(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + + RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + + PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND,KBOUT,IOK) + END IF + IF(IOK.NE.0) RETURN +100 CONTINUE + IF(IFOUND.GT.0) THEN + CALL BUB2AS(PSAVE,NSAVE,IFOUND) + CALL PETER(SEQ,IDSEQ,J1,J2,KSTART, + + NAMES,IDNAML,NAMEP,NAMLEN,MAXEN, + + PSAVE,NSAVE,IDEV,IFOUND,LINLEN,PAA,ITRAN) + ELSE + WRITE(IDEV,1010) +1010 FORMAT(' No matches') + END IF + END + SUBROUTINE S1D0(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + +RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + +PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND,POSN,WORDP,IDNDEX, + +CONST,LENW,LENC,KBOUT,IOK) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),RECSEQ(IDRSEQ),TEMP3(40) + INTEGER RECSTR(MAXEN),PSAVE(MAXMAT),NSAVE(MAXMAT) + INTEGER PCUT(MAXEN),CUTSIT(MAXS),CUTADD(MAXMAT),SPOINT + INTEGER LENEN(MAXS) + INTEGER POSN(IDSEQ),WORDP(IDNDEX),CONST(0:LENC),GENRCI + EXTERNAL MC,GENRCI + IOK = 1 + LREC = 0 + DO 50 IREC=1,NREC +C POINT TO START OF STRING + ISTR=RECSTR(JEN)+(IREC-1)*(LREC) + SPOINT = PCUT(JEN) + IREC - 1 + LREC = LENEN (SPOINT) +C FIND FIRST AND LAST NON N CHARS IN STRING + CALL GETNON(RECSEQ(ISTR),LREC,INON,LNON) +C CHECK FOR ALL N'S IN STRING!! + IF(LNON.GT.0)THEN + ISTR = ISTR + INON - 1 + IENTRY = 0 +5 CONTINUE + NINDEX = GENRCI(RECSEQ(ISTR),LNON,LENW,CONST,LENC,IENTRY) + IF(NINDEX.NE.0) THEN + K1 = WORDP(NINDEX) + IF(K1.NE.0) THEN + IMATCH = 0 + K2 = K1 + J1 - 1 + IF(LNON.GT.LENW) THEN + IF((K2+LNON-1).LE.IDSEQ) THEN + IMATCH = MC(SEQ(K2+LENW),RECSEQ(ISTR+LENW), + + LNON-LENW) + ELSE + IMATCH = 1 + END IF + END IF + IF(IMATCH.EQ.0) THEN + IFOUND = IFOUND + 1 + IF(IFOUND.GT.MAXMAT)THEN + CALL ERROM(KBOUT,'Too many matches') + RETURN + END IF + NSAVE(IFOUND) = JEN +C NEED THE CUTPOINT FOR THIS STRING; THE FIRST ONE THIS NAME +C IS AT PCUT(JEN) IN ARRAY CUTSIT, SO THIS ONE IS AT +C PCUT(JEN)+IREC-1 = SPOINT IN CUTSIT + CUTADD(IFOUND) = SPOINT +C SAVE POSITION RELATIVE TO ARRAY + KT = K2 + CUTSIT(SPOINT) - INON + 1 + IF(KT.GT.IDSEQ)KT = KT - IDSEQ + IF(KT.LT.0)KT = ABS(KT) + 1 + PSAVE(IFOUND) = KT + END IF +10 CONTINUE + K3 = K1 + K1 = POSN(K3) + IF(K1.NE.0) THEN + IMATCH = 0 + K2 = K1 + J1 - 1 + IF(LNON.GT.LENW) THEN + IF((K2+LNON-1).LE.IDSEQ) THEN + IMATCH = MC(SEQ(K2+LENW),RECSEQ(ISTR+LENW), + + LNON-LENW) + ELSE + IMATCH = 1 + END IF + END IF + IF(IMATCH.EQ.0) THEN + IFOUND = IFOUND + 1 + IF(IFOUND.GT.MAXMAT)THEN + CALL ERROM(KBOUT,'Too many matches') + RETURN + END IF + NSAVE(IFOUND) = JEN + CUTADD(IFOUND) = SPOINT + KT = K2 + CUTSIT(SPOINT) - INON + 1 + IF(KT.GT.IDSEQ)KT = KT - IDSEQ + IF(KT.LT.0)KT = ABS(KT) + 1 + PSAVE(IFOUND) = KT + END IF + GO TO 10 + END IF + END IF + GO TO 5 + END IF + IF(LNON.GE.IDSEQ)GO TO 50 + IF(IROUND.NE.1)GO TO 50 + IAT = 0 + LNONM1 = LNON - 1 + IF(LNONM1.GT.0)THEN + CALL SQCOPY(SEQ(IDSEQ-LNON+2),TEMP3,LNONM1) + CALL SQCOPY(SEQ,TEMP3(LNON),LNONM1) + END IF +51 CONTINUE + IAT = IAT + 1 + ILEFT = 2 * LNONM1 - IAT + 1 + IF(ILEFT.GT.0)THEN + CALL FIND6(TEMP3(IAT),ILEFT,RECSEQ(ISTR),LNON,INC,IMATCH) + IF(IMATCH.GT.0)THEN + IFOUND = IFOUND + 1 + IF(IFOUND.GT.MAXMAT)THEN + CALL ERROM(KBOUT,'Too many matches') + RETURN + END IF + NSAVE(IFOUND)=JEN +C NEED THE CUTPOINT FOR THIS STRING; THE FIRST ONE THIS NAME +C IS AT PCUT(JEN) IN ARRAY CUTSIT, SO THIS ONE IS AT +C PCUT(JEN)+IREC-1 = SPOINT IN CUTSIT + CUTADD(IFOUND) = SPOINT + IAT = IAT + IMATCH - 1 + KT=IDSEQ - LNON + 1 + IAT + CUTSIT(SPOINT) - INON + 1 + IF(KT.GT.IDSEQ) KT = KT - IDSEQ + IF(KT.LT.1) KT = ABS(KT) + 1 + PSAVE(IFOUND) = KT + END IF + GO TO 51 + END IF + END IF +50 CONTINUE + IOK = 0 + END + SUBROUTINE S1D1(SEQ,IDSEQ,NREC,JEN,IREC,J1,J2,INC,KSTART, + +RECSTR,MAXEN,PSAVE,NSAVE,MAXMAT,RECSEQ,IDRSEQ,IFOUND, + +PCUT,CUTSIT,LENEN,MAXS,CUTADD,IROUND,KBOUT,IOK) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),RECSEQ(IDRSEQ),TEMP3(40) + INTEGER RECSTR(MAXEN),PSAVE(MAXMAT),NSAVE(MAXMAT) + INTEGER PCUT(MAXEN),CUTSIT(MAXS),CUTADD(MAXMAT),SPOINT + INTEGER LENEN(MAXS) + IOK = 1 + LREC = 0 + DO 50 IREC=1,NREC +C POINT TO START OF STRING + ISTR=RECSTR(JEN)+(IREC-1)*(LREC) +C NOTE WE NEED TO POINT TO THE CORRECT ARRAY ELEMENT BUT +C TO REMEMBER THE SEQUENCE ELEMENT FOR OUTPUT +C IAT IS THE ARRAY ELEMENT + IAT = J1-INC-KSTART+1 + SPOINT = PCUT(JEN) + IREC - 1 + LREC = LENEN (SPOINT) +C FIND FIRST AND LAST NON N CHARS IN STRING + CALL GETNON(RECSEQ(ISTR),LREC,INON,LNON) +C CHECK FOR ALL N'S IN STRING!! + IF(LNON.GT.0)THEN + ISTR = ISTR + INON - 1 +10 CONTINUE + IAT = IAT+INC + ILEFT = J2-IAT+1-KSTART+1 + IF(ILEFT.GT.0)THEN + CALL FIND7(SEQ(IAT),ILEFT, + + RECSEQ(ISTR),LNON,INC,IMATCH) + IF(IMATCH.GT.0)THEN + IFOUND = IFOUND + 1 + IF(IFOUND.GT.MAXMAT)THEN + CALL ERROM(KBOUT,'Too many matches') + RETURN + END IF + IAT = IAT + IMATCH - 1 + NSAVE(IFOUND) = JEN +C NEED THE CUTPOINT FOR THIS STRING; THE FIRST ONE THIS NAME +C IS AT PCUT(JEN) IN ARRAY CUTSIT, SO THIS ONE IS AT +C PCUT(JEN)+IREC-1 = SPOINT IN CUTSIT + CUTADD(IFOUND) = SPOINT +C SAVE POSITION RELATIVE TO ARRAY + KT = IAT + CUTSIT(SPOINT) - INON + 1 + IF(KT.GT.IDSEQ)KT = KT - IDSEQ + IF(KT.LT.0)KT = ABS(KT) + 1 + PSAVE(IFOUND) = KT + GO TO 10 + END IF + END IF + IF(LNON.GE.IDSEQ)GO TO 50 + IF(IROUND.NE.1)GO TO 50 + IAT = 0 + LNONM1 = LNON - 1 + IF(LNONM1.GT.0)THEN + CALL SQCOPY(SEQ(IDSEQ-LNON+2),TEMP3,LNONM1) + CALL SQCOPY(SEQ,TEMP3(LNON),LNONM1) + END IF +51 CONTINUE + IAT = IAT + 1 + ILEFT = 2 * LNONM1 - IAT + 1 + IF(ILEFT.GT.0)THEN + CALL FIND7(TEMP3(IAT),ILEFT,RECSEQ(ISTR),LNON,INC,IMATCH) + IF(IMATCH.GT.0)THEN + IFOUND = IFOUND + 1 + IF(IFOUND.GT.MAXMAT)THEN + CALL ERROM(KBOUT,'Too many matches') + RETURN + END IF + NSAVE(IFOUND)=JEN +C NEED THE CUTPOINT FOR THIS STRING; THE FIRST ONE THIS NAME +C IS AT PCUT(JEN) IN ARRAY CUTSIT, SO THIS ONE IS AT +C PCUT(JEN)+IREC-1 = SPOINT IN CUTSIT + CUTADD(IFOUND) = SPOINT + IAT = IAT + IMATCH - 1 + KT=IDSEQ - LNON + 1 + IAT + CUTSIT(SPOINT) - INON + 1 + IF(KT.GT.IDSEQ) KT = KT - IDSEQ + IF(KT.LT.1) KT = ABS(KT) + 1 + PSAVE(IFOUND) = KT + END IF + GO TO 51 + END IF + END IF +50 CONTINUE + IOK = 0 + END + SUBROUTINE DECREN(RENZYM,IDREN,RECSEQ,IDRSEQ,NAMES,IDNAML, + +NAMEP,NAMLEN,NUMREC,LENEN,RECSTR,MAXEN,KBOUT,CUTSIT,PCUT,MAXS) +C ROUTINE TO DECODE A RESTRICTION ENZYME FILE ARRAY RENZYM IDREN +C INTO THE FOLLOWING: +C A CONCATENATED ARRAY OF RECOGNITION SEQUENCES RECSEQ IDRSEQ PREC +C A CONCATENATED ARRAY OF ENZYME NAMES NAMES IDNAML,PNAM +C A LIST OF POINTERS TO ENZYME NAMES IN NAMES NAMEP MAXEN ITOTEN +C A LIST OF ENZYME NAME LENGTHS NAMLEN MAXEN ITOTEN +C A LIST OF NUMBERS OF RECOGNITION SEQUENCES PER ENZYME NUMREC MAXEN +C A LIST OF LENGHTS OF RECOGNITION SEQUENCES PER RECOGNITION SEQUENCE +C LENEN MAXEN ITOTEN +C A LIST OF POINTERS TO THE FIRST CHARACTER OF THE FIRST RECOGNITION +C SEQUENCE PER ENZYME RECSTR MAXEN ITOTEN +C A LIST OF CUTSITES PER SEQUENCE CUTSIT +C A LIST OF START POSITIONS FOR CUTSITES PER NAME PCUT (ALSO USED +C FOR LENGTHS OF RECOGNITION SEQUENCES) +C AUTHOR: RODGER STADEN + CHARACTER RENZYM(IDREN),RECSEQ(IDRSEQ),NAMES(IDNAML) + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),NUMREC(MAXEN),LENEN(MAXEN) + INTEGER RECSTR(MAXEN),TOTEN,PREC,PREN,PNAM + INTEGER CUTSIT(MAXEN),PCUT(MAXEN) + CHARACTER CUT + EXTERNAL INDEXA + SAVE CUT + DATA CUT/''''/ +C NB CUTSIT SHOULD REALLY BE DIMENSIONED TO BE LARGER THAN THE +C MAX NUMBER OF ENZYMES AS IT WILL BE LONGER +C +C set enzyme count to zero + TOTEN=0 +C point to string store + PREC=1 +C point to renzym + PREN=0 +C point to names array + PNAM=1 +C SET NUMBER OF CUTPOINTS TO ZERO. IT IS EQUAL TO THE NUMBER OF +C OF RECOGNITION SEQUENCES. NOTE THEY CAN ALMOST ALL BE CODED AS SINGLE +C SEQUENCES USING THE NC-IUB CODES, BUT SOMETIMES, IF THE CUTSITE IS +C NON-SYMMETRICAL TWO ARE NEEDED + ICUT = 0 +C +10 CONTINUE + PREN=PREN+1 + IF(PREN.LT.IDREN)THEN +C some data left +C should be pointing at start of name, count its chars + CALL CHARCT(RENZYM,IDREN,PREN,NCHAR) + IF(NCHAR.GT.0)THEN +C found a name +C increment number of enzymes + TOTEN=TOTEN+1 + IF(TOTEN.LT.MAXEN)THEN +C space left for enzymes + IF((PNAM+NCHAR-1).GT.IDNAML)THEN + CALL ERROM(KBOUT,'Names array full') + GO TO 101 + END IF +C space for name +C store name start position, length of name + NAMEP(TOTEN)=PNAM + NAMLEN(TOTEN)=NCHAR + CALL SQCOPY(RENZYM(PREN),NAMES(PNAM),NCHAR) + PNAM=PNAM+NCHAR +C count number of strings this enzyme + NREC=0 +20 CONTINUE +C point to first char of string + PREN=PREN+NCHAR+1 +C count chars in string + NCHAR=0 + IF(PREN.LT.IDREN)CALL CHARCT(RENZYM,IDREN,PREN,NCHAR) + IF(NCHAR.EQ.0)THEN +C no chars in string. If first string then error, else no more strings + IF(NREC.EQ.0)THEN + CALL ERROM(KBOUT,'Name with no strings') +C decrease number of enzymes + TOTEN=TOTEN-1 + GO TO 100 + END IF +C some strings +C save number of strings this enzyme + NUMREC(TOTEN)=NREC +C + GO TO 10 + END IF +C nonzero string length + NREC=NREC+1 +****************************************** +C SAVE ITS START POSITION IN RECSEQ IF FIRST THIS NAME + IF(NREC.EQ.1)RECSTR(TOTEN)=PREC +C FIND CUTSITE + JCUT=INDEXA(RENZYM(PREN),NCHAR,CUT) +C IF JCUT IS ZERO THEN NO CUTSITE MARKED + IF(ICUT.GE.MAXEN)GO TO 200 + ICUT = ICUT + 1 + CUTSIT(ICUT) = 0 +C IF FIRST CUTSITE THIS NAME SAVE ITS POINTER + IF(NREC.EQ.1)PCUT(TOTEN) = ICUT + IF(JCUT.GT.0)THEN + NL = JCUT - 1 + NR = NCHAR - JCUT + CUTSIT(ICUT) = NL +C COPY UP TO CUT + IF(NL.GT.0)THEN + CALL SQCOPY(RENZYM(PREN),RECSEQ(PREC),NL) + PREC = PREC + NL + END IF +C COPY AFTER CUT + IF(NR.GT.0)THEN + IPREN = PREN + NL + 1 + CALL SQCOPY(RENZYM(IPREN),RECSEQ(PREC),NR) + PREC = PREC + NR + END IF + LENEN(ICUT) = NCHAR - 1 +C (REDUCED NCHAR BY 1 TO ALLOW FOR CUT) + ELSE +C +C NO CUT SITE MARKED, USE OLD CODE +****************************************** +C copy string to recseq + CALL SQCOPY(RENZYM(PREN),RECSEQ(PREC),NCHAR) +C + PREC=PREC+NCHAR + LENEN(ICUT) = NCHAR + END IF +C now look for next string + GO TO 20 + END IF +C too many enzymes + CALL ERROM(KBOUT,'Too many names') + GO TO 100 + END IF +C deal with name of zero length + CALL ERROM(KBOUT,'Error in names and strings file') + END IF +C now at end of file +100 CONTINUE +101 CONTINUE +C return length of names array + IDNAML=PNAM-1 +C return number of enzymes + MAXEN=TOTEN +C return length of recseq + IDRSEQ=PREC +C RETURN NUMBER OF RECOGNITION SEQUENCES + MAXS = ICUT + RETURN +200 CONTINUE +C COME HERE WITH OVERFLOW OF CUTSITES +C +C REDUCE NUMBER OF NAMES BY 1 + MAXEN = TOTEN - 1 + IDNAML = PNAM - 1 + IDRSEQ = PREC +C RETURN NUMBER OF RECOGNITION SEQUENCES + MAXS = ICUT + END + SUBROUTINE GETREN(RENZYM,IDREN,IDEV,FILNAM,FILE4,FILE6,FILEA, + + IOK,KBIN,KBOUT,IFILE,DIALOG, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),FILE4*(*),FILE6*(*),FILEA*(*),RENZYM(IDREN) + INTEGER DIALOG + PARAMETER (MAXPRM = 16) + CHARACTER PROMPT(5)*(MAXPRM) + IOK = 1 + IF(DIALOG.EQ.0) NOPT = IFILE + IOP = 1 +1 CONTINUE + IF(DIALOG.EQ.1) THEN + IOP = 1 + PROMPT(1) = 'Search' + PROMPT(2) = 'List enzyme file' + PROMPT(3) = 'Clear text' + PROMPT(4) = 'Clear graphics' + CALL RADION('Select operation',PROMPT,4,IOP, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.1) THEN + IOK = 1 + RETURN + END IF + IF(IOP.EQ.3) THEN + CALL CLEARV + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + GO TO 1 + END IF + IF(IOP.EQ.4) THEN + CALL CLEARG + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + GO TO 1 + END IF + IF((IOP.EQ.1).OR.(IOP.EQ.2)) THEN + NOPT = IFILE + PROMPT(1) = 'All enzymes file' + PROMPT(2) = 'Six cutter file' + PROMPT(3) = 'Four cutter file' + PROMPT(4) = 'Personal file' + PROMPT(5) = 'Keyboard' + CALL RADION('Select input source',PROMPT,5,NOPT, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(NOPT.LT.1) THEN + IOK = 1 + RETURN + END IF + END IF + END IF + IF(NOPT.EQ.3)THEN + CALL OPENRS(IDEV,FILE4,IOK,LRECL,2) + IF(IOK.NE.0)GO TO 100 + IF(IOP.EQ.2)THEN + CALL TTEXT1(IDEV,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IQUIT) + GO TO 1 + END IF + CALL ARRFIL(IDEV,RENZYM,IDREN,KBOUT) + CLOSE(UNIT=IDEV) + RETURN + END IF + IF(NOPT.EQ.2)THEN + CALL OPENRS(IDEV,FILE6,IOK,LRECL,2) + IF(IOK.NE.0)GO TO 100 + IF(IOP.EQ.2)THEN + CALL TTEXT1(IDEV,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IQUIT) + GO TO 1 + END IF + CALL ARRFIL(IDEV,RENZYM,IDREN,KBOUT) + CLOSE(UNIT=IDEV) + RETURN + END IF + IF(NOPT.EQ.1)THEN + CALL OPENRS(IDEV,FILEA,IOK,LRECL,2) + IF(IOK.NE.0)GO TO 100 + IF(IOP.EQ.2)THEN + CALL TTEXT1(IDEV,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IQUIT) + GO TO 1 + END IF + CALL ARRFIL(IDEV,RENZYM,IDREN,KBOUT) + CLOSE(UNIT=IDEV) + RETURN + END IF + IF(NOPT.EQ.4)THEN +10 CONTINUE + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + + 'Restriction enzyme file', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.EQ.2) GO TO 1 + IF(IOK.NE.0)GO TO 100 + IF(IOP.EQ.2)THEN + CALL TTEXT1(IDEV,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IQUIT) + GO TO 1 + END IF + CALL ARRFIL(IDEV,RENZYM,IDREN,KBOUT) + CLOSE(UNIT=IDEV) + RETURN + END IF + IF(NOPT.EQ.5)THEN + CALL GETRKB(RENZYM,IDREN,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH, + + IOK) + RETURN + END IF + GO TO 1 +100 CONTINUE + CALL ERROM(KBOUT,'Error opening file') + IOK=1 + GO TO 1 + END + SUBROUTINE FIND6(SEQ,IDIM1,STRING,IDIM2,INC,IMATCH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER PSEQ,PSTR + EXTERNAL IUBM1 + PSEQ=1-1*INC + PSTR=1 + IMATCH=0 +100 CONTINUE + PSEQ=PSEQ+1-PSTR + PSEQ=PSEQ+INC-1 + PSTR=0 +500 CONTINUE + PSEQ=PSEQ+1 + IF(PSEQ.GT.IDIM1)RETURN + PSTR=PSTR+1 + IUBMAT = IUBM1(STRING(PSTR),SEQ(PSEQ)) + IF(IUBMAT.EQ.0)GO TO 100 + IF(PSTR.LT.IDIM2)GO TO 500 + IMATCH=PSEQ-IDIM2+1 + END + SUBROUTINE FIND7(SEQ,IDIM1,STRING,IDIM2,INC,IMATCH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER PSEQ,PSTR + EXTERNAL IUBM2 + PSEQ=1-1*INC + PSTR=1 + IMATCH=0 +100 CONTINUE + PSEQ=PSEQ+1-PSTR + PSEQ=PSEQ+INC-1 + PSTR=0 +500 CONTINUE + PSEQ=PSEQ+1 + IF(PSEQ.GT.IDIM1)RETURN + PSTR=PSTR+1 + IUBMAT = IUBM2(STRING(PSTR),SEQ(PSEQ)) + IF(IUBMAT.EQ.0)GO TO 100 + IF(PSTR.LT.IDIM2)GO TO 500 + IMATCH=PSEQ-IDIM2+1 + END + SUBROUTINE GETNON(STRING,IDS,LNOTN,RNOTN) +C AUTHOR: RODGER STADEN + CHARACTER STRING(IDS),CHAR + INTEGER RNOTN + EXTERNAL NNDEXL,NNDEXR + PARAMETER (CHAR='N') +C FIND ENDS OF CHARACTER STRING THAT ARE NOT N +C RNOTN IS RETURNED AS THE LENGTH OF THE STRING WITHOUT N'S +C IT WILL BE < 1 IF THE WHOLE STRING IS N'S + LNOTN = NNDEXL(STRING,IDS,CHAR) + RNOTN = NNDEXR(STRING,IDS,CHAR) + RNOTN = RNOTN - LNOTN + 1 + END + INTEGER FUNCTION NNDEXL(STRING,ID,CHAR) + CHARACTER STRING(ID),CHAR +C FUNCTION TO FIND FIRST NON-OCCURRENCE OF CHAR IN STRING + DO 10 I = 1,ID + IF(STRING(I).NE.CHAR)THEN + NNDEXL = I + RETURN + END IF +10 CONTINUE + NNDEXL = ID + 1 + END + INTEGER FUNCTION NNDEXR(STRING,ID,CHAR) + CHARACTER STRING(ID),CHAR +C FUNCTION TO FIND FIRST NON-OCCURRENCE OF CHAR IN STRING + DO 10 I = ID,1,-1 + IF(STRING(I).NE.CHAR)THEN + NNDEXR = I + RETURN + END IF +10 CONTINUE + NNDEXR = 0 + END + SUBROUTINE S2(SEQ,IDSEQ,IFOUND,NAMEP,NAMLEN,LENEN,MAXEN, + +NSAVE,PSAVE,LENGTH,MAXMAT,NAMES,IDNAML, + +CUTSIT,MAXS,CUTADD,IROUND, + +LENN,IDEV,J2,KSTART,JOB) +C 19-2-91 Changed idt and idtrs from 20 each to 15 and 35 +C so names have max length 15 chars and recognition seqs 35 +C for this part of the code. + PARAMETER (IDT = 15, IDTRS = 35) + CHARACTER SEQ(IDSEQ),TEMP1(IDT),TEMP2(IDTRS),NAMES(IDNAML),CUTSYM + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),LENEN(MAXS) + INTEGER NSAVE(MAXMAT),PSAVE(MAXMAT),LENGTH(MAXMAT) + INTEGER CUTSIT(MAXS),CUTADD(MAXMAT) + EXTERNAL LWRAP3 + SAVE CUTSYM + DATA CUTSYM/''''/ + WRITE(IDEV,1011) +1011 FORMAT( + +' Name Sequence', + +' Position', + +' Fragment lengths') + DO 60 KOUT=1,IFOUND + KR1=NAMEP(NSAVE(KOUT)) + KS1=PSAVE(KOUT) - CUTSIT(CUTADD(KOUT)) + CALL FILLC(TEMP1,IDT,' ') + CALL FILLC(TEMP2,IDTRS,' ') + KR2 = NAMLEN(NSAVE(KOUT)) + CALL SQCOPY(NAMES(KR1),TEMP1,KR2) + ITEMP1 = 0 + DO 58 ITEMP = 1,CUTSIT(CUTADD(KOUT)) + ITEMP1 = ITEMP + KS2 = LWRAP3(J2,KS1) + TEMP2(ITEMP) = SEQ(KS2) + KS1 = KS1 + 1 +58 CONTINUE + ITEMP1 = ITEMP1 + 1 + TEMP2(ITEMP1) = CUTSYM + ITEMP1 = ITEMP1 + 1 + DO 59 ITEMP = ITEMP1,LENEN(CUTADD(KOUT))+1 + KS2 = LWRAP3(J2,KS1) + TEMP2(ITEMP) = SEQ(KS2) + KS1 = KS1 + 1 +59 CONTINUE + LENN=PSAVE(KOUT)-LENN + IF(JOB.EQ.0) THEN + WRITE(IDEV,1008)KOUT, + + TEMP1,TEMP2,PSAVE(KOUT)+KSTART-1,LENN,LENGTH(KOUT) + ELSE IF(JOB.EQ.1) THEN + WRITE(IDEV,1008)KOUT, + + TEMP1,TEMP2,PSAVE(KOUT)+KSTART-1,LENGTH(KOUT) + END IF + LENN=PSAVE(KOUT) +1008 FORMAT(' ',I4,' ',15A1,1X,35A1,1X,I6,1X,I6,1X,I6) +60 CONTINUE + IF(IROUND.EQ.0)THEN +C need length to end + LEND = J2-PSAVE(IFOUND)+1-KSTART+1 + IF(JOB.EQ.0)WRITE(IDEV,1020)LEND,LENGTH(IFOUND+1) + IF(JOB.EQ.1)WRITE(IDEV,1020)LEND +1020 FORMAT(' ',64X,I6,1X,I6) + END IF + END + SUBROUTINE PETER(SEQ,IDSEQ,J1,J2,KSTART, + +NAMES,IDNAML,NAMEP,NAMLEN,MAXEN, + +PSAVE,NSAVE,IDEV,IFOUND,LINLEN,PAA,ITRAN) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),NAMES(IDNAML),TEMP1*130 + INTEGER NAMEP(MAXEN),NAMLEN(MAXEN),PSAVE(IFOUND),NSAVE(IFOUND) + CHARACTER TRANF3*3,PAA(125) + EXTERNAL TRANF3 + EXTERNAL NOTIRL +C KSTART = SEQ ELEMENT IN SEQ(1) +C J1 = START OF ACTIVE REGION RELATIVE TO SEQ(1) +C PSAVE ARE RELATIVE TO SEQ(1) +C WORK RELATIVE TO ARRAY SEQ + NLINES = 1 + (J2 - J1)/LINLEN + I1 = J1 + I2 = MIN(J2,I1+LINLEN-1) + NEXTE = 1 + ITMAX = J2 - 2 + DO 100 KK = 1,NLINES + TEMP1 = ' ' + LP1 = 0 + DO 50 I = NEXTE,IFOUND + KS1=PSAVE(I) + IF(KS1.GT.I2)THEN + NEXTES = I + GO TO 51 + END IF + KR1=NAMEP(NSAVE(I)) + LP = KS1 - I1 + 1 +C TRAP FOR CUTSITE OUT OF RANGE!!!!!! + IF(LP.LT.0)THEN + WRITE(IDEV,1004)(NAMES(K),K=KR1,KR1+NAMLEN(NSAVE(I))-1) +1004 FORMAT(' WARNING:', + + ' Recognition site with cut off end for enzyme ',20A1) + WRITE(IDEV,1005) +1005 FORMAT(//) + END IF + IF(LP.GT.0)THEN + IF(LP.LE.LP1)THEN + WRITE(IDEV,1000)TEMP1(1:MAX(1,NOTIRL(TEMP1,130,' '))) +1000 FORMAT(' ',A) + CALL DOTTY(TEMP1) + END IF + DO 66 ITEMP=1,NAMLEN(NSAVE(I)) + TEMP1(LP:LP)=NAMES(KR1) + KR1=KR1+1 + LP = LP + 1 +66 CONTINUE + LP1 = LP + END IF + NEXTES = I + 1 +50 CONTINUE +51 CONTINUE + WRITE(IDEV,1000)TEMP1(1:MAX(1,NOTIRL(TEMP1,130,' '))) + NEXTE = NEXTES + WRITE(IDEV,1001)(SEQ(K),K=I1,I2) + WRITE(IDEV,1002)(K,K=I1+9+KSTART-1,I2+KSTART-1,10) + IF(ITRAN.NE.1)THEN + WRITE(IDEV,1010)(TRANF3(SEQ(K),PAA,ITRAN), + + K=I1,MIN(ITMAX,I2-1),3) + WRITE(IDEV,1011)(TRANF3(SEQ(K),PAA,ITRAN), + + K=I1+1,MIN(ITMAX,I2),3) + WRITE(IDEV,1012)(TRANF3(SEQ(K),PAA,ITRAN), + + K=I1+2,MIN(ITMAX,I2+1),3) + END IF + WRITE(IDEV,1003) +1003 FORMAT(' ') +1002 FORMAT(' ',10I10) +1001 FORMAT(' ',100A1) +1010 FORMAT(1X,40(A)) +1011 FORMAT(2X,40(A)) +1012 FORMAT(3X,40(A)) + I1 = I2 + 1 + I2 = MIN((I1+LINLEN-1),J2) +100 CONTINUE + END + SUBROUTINE DOTTY(TEMP) + CHARACTER TEMP*(*),DOT + PARAMETER (DOT='.') + DO 10 I = LEN(TEMP),2,-1 + IF(TEMP(I:I).NE.' ')THEN + IF(TEMP(I:I).NE.DOT)THEN + IF((TEMP(I-1:I-1).EQ.' ').OR.(TEMP(I-1:I-1).EQ.DOT))THEN + TEMP(I:I) = DOT + ELSE + TEMP(I:I) = ' ' + END IF + END IF + END IF +10 CONTINUE + IF(TEMP(1:1).NE.' ')TEMP(1:1) = DOT + END + SUBROUTINE SEQFIT(SEQNCE,IDIM1,SEQ2,IDIM3I,ITOT,ITOTEL,ITOTID, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART, + +IDEV,IDEVIN,FILE2,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,DIALOG) +C AUTHOR: RODGER STADEN + INTEGER DIALOG + PARAMETER (MAXSTR = 50) + CHARACTER HELPF*(*) + CHARACTER SEQNCE(IDIM1),STRING(MAXSTR) + CHARACTER SEQ2(IDIM3I),FILE2*(*) + CHARACTER MATCH(MAXSTR) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + INTEGER ANSTY + CALL SHOWFU(KBOUT,'Find percentage matches') + IDIM2 = 0 + IDIM2I = MAXSTR + IDIM3 = IDIM3I + IS = J1 + IE = J2 + ISS = 1 + ISE = 10 + CALL GETPAR(18,2,IOK,ANSTY,IPR, + +IPAR3,IPAR4,IPAR5, + +IPAR6,IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + PR = REAL(IPR) +5 CONTINUE + IF(DIALOG.EQ.1) THEN + CALL SQPFD1(SEQ2,IDIM3I,IDIM3,ANSTY,IDEVIN,FILE2, + + KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF +10 CONTINUE + CALL SQPFD2(SEQ2,IDIM3,MATCH,STRING, + +J1,J2,KSTART,ISS,ISE,IS,IE,IDIM2I,IDIM2,MAXSTR,PR,ANSTY,MINP, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL SQPF6(SEQNCE,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,KSTART,J1,J2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IOK) + IF(IOK.EQ.2)THEN + WRITE(KBOUT,1017)ITOTID +1017 FORMAT(/,' More than',I6,' matches.', + + ' Try changing percentage or region'/) + GO TO 10 + END IF + IF(IOK.NE.0) RETURN + CALL SQPF7(SEQNCE,IDIM1,STRING,IDIM2,MATCH,ITOT,ITOTEL,ITOTID, + +ITOTP,KSTART,J1,J2,ISS,PR,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + GO TO 10 + END + SUBROUTINE SQPFD2(SEQ2,IDIM3,MATCH,STRING, + +J1,J2,KSTART,ISS,ISE,IS,IE,IDIM2I,IDIM2,MAXSTR,PR,ANSTY,MINP, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + CHARACTER STRING(MAXSTR) + CHARACTER SEQ2(IDIM3) + CHARACTER MATCH(MAXSTR) + INTEGER ANSC,ANSTY + IOK = 1 + CALL YESNO(ANSC,'Keep picture', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANSC.LT.0)RETURN + IF(ANSC.EQ.1)CALL CLEARG + IF(ANSTY.EQ.1) THEN + CALL SQPF2(SEQ2,IDIM3,STRING,IDIM2I,IDIM2,ISS,ISE, + + KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + IF(ANSTY.EQ.0) THEN + CALL SQPF3(STRING,MATCH,IDIM2I,IDIM2, + + KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL SQPF8(STRING,IDIM2, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN +C CALL SQPF4(KBIN,KBOUT,J1,J2,IS,IE, +C +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C IF(IOK.NE.0) RETURN + CALL SQPF5(IDIM2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PR,MINP,IOK) +C IF(IOK.NE.0) RETURN + RETURN + END + SUBROUTINE SQPF6(SEQNCE,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,KSTART,J1,J2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IOK) + CHARACTER SEQNCE(IDIM1),STRING(IDIM2) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + IOK = 1 + CALL FILLI(ITOT,ITOTID,0) + CALL FILLI(ITOTEL,ITOTID,0) + CALL SQFIT6(SEQNCE,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,KSTART) + IF(ITOTP.GT.ITOTID)THEN + IOK = 2 + RETURN + END IF + IF(ITOTP.GT.0)THEN + XMIN=J1 + XMAX=J2 + YMIN=0. + YMAX=IDIM2 + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + DO 203 I=1,ITOTP + X=ITOTEL(I) + Y=ITOT(I) + CALL LINE(X,X,YMIN,Y,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +203 CONTINUE + END IF + CALL VT100M + IOK = 0 + END + SUBROUTINE SQPF8(STRING,LENGTH, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER STRING(LENGTH) + IOK = 1 + IANSC = 0 + WRITE(KBOUT,1000)(STRING(K),K=1,LENGTH) +1000 FORMAT(' STRING=',50A1) + CALL YESNO(IANSC,'This sense', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IANSC.LT.0)RETURN + IF(IANSC.EQ.1)THEN + CALL SQREV(STRING,LENGTH) + CALL SQCOM2(STRING,LENGTH) + WRITE(KBOUT,1000)(STRING(K),K=1,LENGTH) + END IF + IOK = 0 + END + SUBROUTINE SEPFIT(SEQNCE,IDIM1,SEQ2,IDIM3I,ITOT,ITOTEL,ITOTID, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART, + +IDEV,IDEVIN,FILE2,KBIN,KBOUT,MATRIX,IDM, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) +C AUTHOR: RODGER STADEN + INTEGER DIALOG + PARAMETER (MAXSTR = 50) + CHARACTER HELPF*(*) + CHARACTER SEQNCE(IDIM1),STRING(MAXSTR) + CHARACTER SEQ2(IDIM3I),FILE2*(*) + CHARACTER MATCH(MAXSTR) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID),MATRIX(IDM,IDM) + INTEGER ANSTY,SMIN,SMAX + CALL SHOWFU(KBOUT,'Find matches using a score matrix') + MINP = 0 + IDIM2 = 0 + IDIM2I = MAXSTR + IDIM3 = IDIM3I + IS = J1 + IE = J2 + ISS = 1 + ISE = 10 + CALL GETPAR(19,1,IOK,ANSTY, + +IPAR2,IPAR3,IPAR4,IPAR5, + +IPAR6,IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN +5 CONTINUE + IF(DIALOG.EQ.1) THEN + CALL SQPFD1(SEQ2,IDIM3I,IDIM3,ANSTY,IDEVIN,FILE2, + + KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF +10 CONTINUE + CALL SEPFD2(SEQ2,IDIM3,MATCH,STRING, + +J1,J2,KSTART,ISS,ISE,IS,IE,IDIM2I,IDIM2,MAXSTR,ANSTY,MINP, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,MATRIX,IDM, + +SMIN,SMAX,IOK) + IF(IOK.NE.0) RETURN + CALL SEPF6(SEQNCE,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,KSTART,J1,J2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,MATRIX,IDM, + +SMIN,SMAX,IOK) + IF(IOK.EQ.2)THEN + WRITE(KBOUT,1017)ITOTID +1017 FORMAT(/,' More than',I6,' matches.', + + ' Try changing score or region'/) + GO TO 10 + END IF + IF(IOK.NE.0) RETURN + CALL SEPF7(SEQNCE,IDIM1,STRING,IDIM2,MATCH,ITOT,ITOTEL,ITOTID, + +ITOTP,MINP,KSTART,J1,J2,ISS,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + GO TO 10 + END + SUBROUTINE SEPFD2(SEQ2,IDIM3,MATCH,STRING, + +J1,J2,KSTART,ISS,ISE,IS,IE,IDIM2I,IDIM2,MAXSTR,ANSTY,MINP, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,MATRIX,IDM, + +SMIN,SMAX,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + CHARACTER STRING(MAXSTR) + CHARACTER SEQ2(IDIM3) + CHARACTER MATCH(MAXSTR) + INTEGER ANSC,ANSTY,MATRIX(IDM,IDM),SMIN,SMAX + IOK = 1 + CALL YESNO(ANSC,'Keep picture', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANSC.LT.0)RETURN + IF(ANSC.EQ.1)CALL CLEARG + IF(ANSTY.EQ.1) THEN + CALL SQPF2(SEQ2,IDIM3,STRING,IDIM2I,IDIM2,ISS,ISE, + + KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + IF(ANSTY.EQ.0) THEN + CALL SQPF3(STRING,MATCH,IDIM2I,IDIM2, + + KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL SQPF8(STRING,IDIM2, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN +C CALL SEPF4(KBIN,KBOUT,J1,J2,IS,IE, +C +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C IF(IOK.NE.0) RETURN + CALL SEPF5(IDIM2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,MINP,MATRIX,IDM, + +SMIN,SMAX,STRING,IOK) +C IF(IOK.NE.0) RETURN + RETURN + END + SUBROUTINE SEPF5(IDIM2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,MINP,MATRIX,IDM, + +SMIN,SMAX,STRING,IOK) + CHARACTER HELPF*(*) + CHARACTER STRING(IDIM2) + INTEGER MATRIX(IDM,IDM),SMIN,SMAX,VALUE + IOK = 1 + CALL GTSCR(STRING,IDIM2,MATRIX,IDM,SMIN,SMAX) + WRITE(KBOUT,1000)SMIN,SMAX +1000 FORMAT(' Minimum score=',I6,' Maximum score=',I6) + MININ = SMIN + MAXIN = SMAX + IF(MINP.EQ.0) MINP = SMAX + CALL GETINT(MININ,MAXIN,MINP,'Score',VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINP = VALUE + IOK = 0 + END + SUBROUTINE SEPF6(SEQNCE,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,KSTART,J1,J2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,MATRIX,IDM, + +SMIN,SMAX,IOK) + CHARACTER SEQNCE(IDIM1),STRING(IDIM2) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID),MATRIX(IDM,IDM) + INTEGER SMIN,SMAX + IOK = 1 + CALL FILLI(ITOT,ITOTID,0) + CALL FILLI(ITOTEL,ITOTID,0) + CALL SPFIT(SEQNCE,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,MATRIX,IDM,KSTART) + IF(ITOTP.GT.ITOTID)THEN + IOK = 2 + RETURN + END IF + IF(ITOTP.GT.0)THEN + XMIN = J1 + XMAX = J2 + YMIN = SMIN + YMAX = SMAX + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + DO 203 I=1,ITOTP + X=ITOTEL(I) + Y=ITOT(I) + CALL LINE(X,X,YMIN,Y,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +203 CONTINUE + END IF + CALL VT100M + IOK = 0 + END +C SQFITP + SUBROUTINE SQFITP(SEQ,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,KSTART) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + IDIF=(IE-IS+2)-IDIM2 + IPSEQ=IS-KSTART+1 + ITOTP=0 + DO 200 I=1,IDIF + NTOT=0 + IP=IPSEQ + DO 100 J=1,IDIM2 + IF(SEQ(IP).EQ.STRING(J))THEN + NTOT=NTOT+1 + ELSE IF (STRING(J).EQ.'-')THEN + NTOT=NTOT+1 + END IF + IP=IP+1 +100 CONTINUE + IF(NTOT.GE.MINP)THEN + ITOTP=ITOTP+1 + IF(ITOTP.GT.ITOTID)RETURN + ITOT(ITOTP)=NTOT + ITOTEL(ITOTP)=IP-IDIM2+KSTART-1 + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + END + SUBROUTINE SIGNAL(SEQ,IDIM,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,IDEV,FILNAM, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IDEVOT, + +SUM,WT,TOT,IDM,MAXLEN,LINE,CHRSET,DIALOG,JOB) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),FILNAM*(*),HELPF*(*) + CHARACTER LINE(MAXLEN),CHRSET(IDM) + INTEGER SUM(IDM,MAXLEN),TOT(MAXLEN),DIALOG + REAL WT(IDM,MAXLEN) + CALL SHOWFU(KBOUT,'Motif search using weight matrix') +C IF(JOB.EQ.2)CALL SHOWFU(KBOUT,'on complementary strand') + CALL GETPAR(20,1,IOK,IGON, + +IPAR2,IPAR3,IPAR4,IPAR5, + +IPAR6,IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + CALL SIGNLD(IDEV,FILNAM, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH, + +SUM,IDM,MAXLEN,TOT,WT,LENGTH,MIDDLE,YMIN,YMAX, + +IGON,JOB,IDEVOT,LINE,CHRSET,DIALOG,IOK) + IF(IOK.NE.0) RETURN + CALL SIGNLP(SEQ,IDIM,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,IDEVOT,WT,IDM,MAXLEN,LENGTH, + +YMIN,YMAX,IGON,MIDDLE) + END + SUBROUTINE SIGNLD(IDEV,FILNAM, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH, + +SUM,IDM,MAXLEN,TOT,WT,LENGTH,MIDDLE,YMIN,YMAX, + +IGON,JOB,IDEVOT,LINE,CHRSET,DIALOG,IOK) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),HELPF*(*),LINE(MAXLEN),CHRSET(IDM) + INTEGER SUM(IDM,MAXLEN),TOT(MAXLEN),DIALOG + REAL WT(IDM,MAXLEN) + PARAMETER (MAXPRM = 21) + CHARACTER PROMPT(3)*(MAXPRM) + IOK = 1 + IDO = 1 + PROMPT(1) = 'Use weight matrix' + PROMPT(2) = 'Make weight matrix' + PROMPT(3) = 'Rescale weight matrix' + CALL RADION('Select operation',PROMPT,3,IDO, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IDO.LT.1) RETURN + IF(IDO.GT.1) THEN + CALL MKWT(WT,SUM,TOT,CHRSET,IDM,MAXLEN, + + IDEV,IDEVOT,KBIN,KBOUT,LINE, + + FILNAM,IDO,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IOK = 1 + RETURN + END IF + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + +'Motif weight matrix file', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + LENGTH=MAXLEN + CALL RDWMT(TOT,SUM,MIDDLE,LENGTH,MAXLEN,YMIN,YMAX,IDEV, + + IOK,IDM,KBOUT,0) + IF(IOK.NE.0)THEN + CALL ERROM(KBOUT,'Error in weight matrix') + RETURN + END IF + IF(DIALOG.EQ.1) THEN + AMN = -9999. + AMX = 9999. + CALL GETRL(AMN,AMX,YMIN,'Cutoff score',VAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + YMIN = VAL + END IF + IF(YMIN.LT.0.0)CALL GETW(TOT,SUM,WT,LENGTH,IDM,MAXLEN) + IF(YMIN.GE.0.0)CALL GETW2(SUM,WT,LENGTH,IDM,MAXLEN) + IF(JOB.EQ.2)CALL COMPWM(WT,LENGTH,MAXLEN,IDM) + CALL YESONO(IGON,'Plot results','List results', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IGON.LT.0)RETURN + IOK = 0 + END + SUBROUTINE SIGNLP(SEQ,IDIM,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,IDEVOT,WT,IDM,MAXLEN,LENGTH, + +YMIN,YMAX,IGON,MIDDLE) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + REAL WT(IDM,MAXLEN) + INTEGER CTONUM + EXTERNAL CTONUM + XMAX=J2 + XMIN=J1 + IF(IGON.EQ.0)THEN + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + DO 10 I=J1,J2-LENGTH+1 + SUM1=0. + K=0 + DO 5 J=I,I+LENGTH-1 + K=K+1 + SUM1=SUM1+WT(CTONUM(SEQ(J)),K) +5 CONTINUE + IF(SUM1.GE.YMIN)THEN + IF(IGON.EQ.0)THEN + X=I+MIDDLE + CALL LINE(X,X,YMIN,SUM1,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + IF(IGON.EQ.1)THEN + CALL VT100M + WRITE(IDEVOT,1010)I+MIDDLE,SUM1,(SEQ(K),K=I,I+LENGTH-1) +1010 FORMAT(' ',I7,F10.2,' ',120A1) + END IF + END IF +10 CONTINUE + CALL VT100M + RETURN + END + SUBROUTINE COMPWM(WTR,LENGTH,MAXL,IDM) +C AUTHOR: RODGER STADEN + REAL WTR(IDM,MAXL) +C COMPLEMENT THE WEIGHT MATRIX + DO 60 I=1,LENGTH + DO 60 J=1,2 + T = WTR(J,I) + WTR(J,I)=WTR(J+2,I) + WTR(J+2,I)=T +60 CONTINUE + L = LENGTH/2 + DO 70 I=1,L + L1 = 1 + LENGTH-I + DO 70 J=1,IDM + T = WTR(J,L1) + WTR(J,L1)=WTR(J,I) + WTR(J,I)=T +70 CONTINUE + END +C AACOMP + SUBROUTINE AACOMP(SUM,IDEV,PAA) +C AUTHOR: RODGER STADEN + REAL SUM(4,4,4),AAWTS(20),EXPERC(20),APERC(20),ASUM(20) + REAL HYDRO(20) + CHARACTER PAA(5,5,5),AA(20) + SAVE AA,AAWTS,EXPERC,HYDRO + DATA AA/'A','C','D','E','F', + +'G','H','I','K','L', + +'M','N','P','Q','R', + +'S','T','V','W','Y'/ + DATA AAWTS/71.09,103.15,115.10,129.13,147.19, + +57.07,137.16,113.17,128.19,113.17, + +131.21,114.12,97.13,128.15,156.20, + +87.09,101.12,99.15,186.23,163.19/ +C ARGOS VALUES + DATA EXPERC/8.3,1.7,5.3,6.2,3.9,7.2,2.2,5.2,5.7, + +9.0,2.4,4.4,5.1,4.0,5.7,6.9,5.8,6.6,1.3,3.2/ +C DAYHOFF VALUES +C DATA EXPERC/8.6,2.9,5.5,6.0,3.6,8.4,2.0,4.5,6.6, +C 17.4,1.7,4.3,5.2,3.9,4.9,7.0,6.1,6.6,1.3,3.4/ + DATA HYDRO/-0.4,0.17,-1.31,-1.22,1.92, + +-0.67,-0.64,1.25,-0.67,1.22, + +1.02,-0.92,-0.49,-0.91,-0.59, + +-0.55,-0.28,0.91,0.50,1.67/ +C hydrophobicity values from r. m. sweet and d. eisenberg, j mol. biol +C (1983) 171, 479-488 +C +C calc amino acid composition + CALL FILLR(ASUM,20,0.0) + TOTAL=0. + ATOT=0. + DO 200 I=1,4 + DO 200 J=1,4 + DO 200 K=1,4 + DO 150 L=1,20 + IF(PAA(K,J,I).EQ.AA(L)) THEN + ASUM(L)=ASUM(L)+SUM(I,J,K) + ATOT=ATOT+SUM(I,J,K) + GO TO 151 + END IF +150 CONTINUE +151 CONTINUE +200 CONTINUE +C set hydrophobicity to zero + HYDROP=0. + DO 300 I=1,20 + HYDROP=HYDROP+ASUM(I)*HYDRO(I) + IF(ATOT.NE.0.)APERC(I)=100.*((100.*ASUM(I)/ATOT)-EXPERC(I)) + + /EXPERC(I) +C botch-up to allow use of dayhoff aawts-18.0 (H2O really=18.015) + TOTAL=TOTAL+ASUM(I)*(AAWTS(I)-0.015) +300 CONTINUE +C add on one water molecule + TOTAL=TOTAL+18.015 + WRITE(IDEV,1002)(AA(K),K=1,10) + WRITE(IDEV,1003)(ASUM(K),K=1,10) + WRITE(IDEV,1005)(APERC(K),K=1,10) + WRITE(IDEV,1002)(AA(K),K=11,20) + WRITE(IDEV,1003)(ASUM(K),K=11,20) + WRITE(IDEV,1005)(APERC(K),K=11,20) +1005 FORMAT(' O-E % ',10F5.0) +1006 FORMAT(' EXP % ',10F4.0) +1002 FORMAT(/' ',10(3X,A1,1X)) +1003 FORMAT(' ',10F5.0) + WRITE(IDEV,1004)ATOT,TOTAL,HYDROP +1004 FORMAT(' Total acids=',F6.0,' Molecular weight=',F10.0, + +' Hydrophobicity=',F6.1) + END +C BCOMPC + SUBROUTINE BCOMPC(SUM,IDEV) +C AUTHOR: RODGER STADEN + REAL SUM(4,4,4),COMP(4,3),COMPO(4,3),BCOMP(4),BCOMPX(4) + CHARACTER BASE(4) + SAVE BASE + DATA BASE/'T','C','A','G'/ +C calc base composition + TCOMP=0. + DO 50 I=1,4 + BCOMP(I)=0. + DO 50 J=1,3 + COMPO(I,J)=0. + COMP(I,J)=0. +50 CONTINUE + DO 55 J=1,4 + DO 55 K=1,4 + DO 55 L=1,4 + TCOMP=TCOMP+SUM(J,K,L) + BCOMP(J)=BCOMP(J)+SUM(J,K,L)+SUM(K,J,L)+SUM(K,L,J) + COMP(J,1)=COMP(J,1)+SUM(J,K,L) + COMP(J,2)=COMP(J,2)+SUM(K,J,L) + COMP(J,3)=COMP(J,3)+SUM(K,L,J) +55 CONTINUE + WRITE(IDEV,1013)TCOMP +1013 FORMAT(' Total codons=',F10.0) +C calc % composition + DO 60 I=1,4 + DO 60 J=1,3 + IF(TCOMP.NE.0.)COMPO(I,J)=COMP(I,J)*100./TCOMP + IF(BCOMP(I).NE.0.)COMP(I,J)=COMP(I,J)*100./BCOMP(I) +60 CONTINUE + WRITE(IDEV,1010)BASE +1010 FORMAT(4(10X,A1)) + WRITE(IDEV,1011)(J,(COMP(I,J),I=1,4),J=1,3) + WRITE(IDEV,1000) +1000 FORMAT(' ',4(' -----')) + WRITE(IDEV,1002) +1002 FORMAT(' =',4(' 100% ')) + WRITE(IDEV,1001)(J,(COMPO(I,J),I=1,4),J=1,3) +1011 FORMAT(3(/2X,I1,(4(F10.2,1X)))) +1001 FORMAT(3(/2X,I1,(4(F10.2,1X)),' = 100%')) + DO 65 I=1,4 + IF(TCOMP.NE.0.)BCOMP(I)=BCOMP(I)*33.3333/TCOMP +65 CONTINUE + WRITE(IDEV,1012)BCOMP +1012 FORMAT(2X,'%',4(F10.2,1X),' Observed, overall totals') +1014 FORMAT(2X,'%',4(F10.2,1X),' Expected, even codons per acid') + CALL BCOMPE(SUM,BCOMPX) + WRITE(IDEV,1014)BCOMPX + END + SUBROUTINE BCOMPE(FABC,BCOMP) +C AUTHOR: RODGER STADEN + INTEGER NCODON(21),IACID(4,4,4) + REAL FABC(4,4,4),FA(21),BCOMP(4) +C array with number of codons per acid + SAVE IACID,NCODON + DATA IACID/ + + 1,2,3,5,6,7,8,9, + + 10,12,14,16,18,20,6,21, + + 1,2,3,5,6,7,8,9, + + 10,12,14,16,18,20,6,21, + + 2,2,3,5,6,7,8,9, + + 11,13,15,17,11,20,20,21, + + 2,2,4,5,6,7,8,9, + + 11,13,15,17,19,20,20,21/ + DATA NCODON/ + + 2,6,3,1,4,6,4,4,4,2,3,2,2,2,2,2,2,2,1,6,4/ + TOTAL=0. + CALL FILLR(BCOMP,4,0.) + CALL FILLR(FA,21,0.) + DO 20 I=1,4 + DO 20 J=1,4 + DO 20 K=1,4 +C point to this acid + JACID=IACID(I,J,K) +C sum its codons + FA(JACID)=FA(JACID)+FABC(I,J,K) + TOTAL=TOTAL+FABC(I,J,K) +20 CONTINUE +C calculate even usage of each acids codons +C need to look up the acids number, its composition is divided by +C its number of codons + DO 21 I=1,4 + DO 21 J=1,4 + DO 21 K=1,4 +C get acid number this codon + JACID=IACID(I,J,K) + BCOMP(I)=BCOMP(I)+FA(JACID)/(REAL(NCODON(JACID))) + BCOMP(J)=BCOMP(J)+FA(JACID)/(REAL(NCODON(JACID))) + BCOMP(K)=BCOMP(K)+FA(JACID)/(REAL(NCODON(JACID))) +21 CONTINUE + DO 22 I=1,4 + IF(TOTAL.NE.0.)BCOMP(I)=BCOMP(I)*33.3333/TOTAL +22 CONTINUE + END + SUBROUTINE CALCOD(SUM,SEQ,IDIM) +C AUTHOR: RODGER STADEN +C calculates codon totals for defined region + REAL SUM(4,4,4) + CHARACTER SEQ(IDIM) + INTEGER CTONUM + EXTERNAL CTONUM + MIN=IDIM/3 + J=1 + DO 100 I=1,MIN +C test for bad chars + DO 50 L=1,3 + IP=J-1+L + IF(CTONUM(SEQ(IP)).EQ.5)GO TO 59 +50 CONTINUE + JP0=J + JP1=J+1 + JP2=J+2 + JP0=CTONUM(SEQ(JP0)) + JP1=CTONUM(SEQ(JP1)) + JP2=CTONUM(SEQ(JP2)) + SUM(JP0,JP1,JP2)=SUM(JP0,JP1,JP2)+1. +59 CONTINUE + J=J+3 +100 CONTINUE + END +C codcom +C complements a codon table + SUBROUTINE CODCOM(SUM) +C AUTHOR: RODGER STADEN + REAL SUM(4,4,4),TEMP(4,4,4) + INTEGER IP(4) + SAVE IP + DATA IP/3,4,1,2/ + DO 10 I=1,4 + DO 10 J=1,4 + DO 10 K=1,4 + IC=IP(I) + JC=IP(J) + KC=IP(K) + TEMP(I,J,K)=SUM(KC,JC,IC) +10 CONTINUE + DO 20 I=1,4 + DO 20 J=1,4 + DO 20 K=1,4 + SUM(I,J,K)=TEMP(I,J,K) +20 CONTINUE + END + SUBROUTINE CODTBL(IDEV,FILNAM,S2, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,ANSE,IOK) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),HELPF*(*) + INTEGER ANSE + REAL S2(64) + IOK = 1 + IOP = ANSE + CALL YESONO(IOP,'Start with empty table', + +'Start with an existing table', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) RETURN + ANSE = IOP + IF(ANSE.EQ.1) THEN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + + 'Name of codon table file', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL REDCOD(S2,IDEV) + CLOSE(UNIT=IDEV) + END IF + IOK = 0 + END + SUBROUTINE LSTSEQ(SEQ,IDIM,IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,J1,J2,IDIMT,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + INTEGER DIALOG +C J1 IS THE FIRST BASE IN THE RAM BUFFER +C J2 IS THE LAST BASE IN THE RAM BUFFER +C IDIM=J2-J1+1 +C IDIMT IS THE ACTUAL SEQUENCE LENGTH +C WRAP AROUND IS ONLY POSSIBLE IF J1=1, AND J2=IDIMT + CALL SHOWFU(KBOUT,'List the sequence') + I1 = J1 + I2 = J2 + LENGTH = 60 + NSTRND = 0 + IF(DIALOG.EQ.1) THEN + CALL LSTSQD(IDIM,KBIN,KBOUT,J1,J2,IDIMT,NSTRND, + + IHELPS,IHELPE,HELPF,IDEVH,I1,I2,LENGTH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL FMTSEQ(SEQ,IDIM,I1,I2,NSTRND,LENGTH,IDEV,J1,IDIMT) + END + SUBROUTINE FMTSEQ(SEQNCE,IDIM,IS1,IE1,NSTRND,LENGTH,IDEV,KSTART, + +IDIMT) +C AUTHOR: RODGER STADEN + CHARACTER SEQNCE(IDIM),SCOMP + INTEGER K(12) + EXTERNAL LWRAP,SCOMP + IS=IS1 + IE=IE1 + LD10=LENGTH/10 + ITOT=IE-IS+1 + IF(IE.LT.IS)ITOT=IDIMT+ITOT + IDONE=0 + N=IS-1 + WRITE(IDEV,1005) +50 CONTINUE + IF(IDONE.GE.ITOT)RETURN + DO 20 I=1,LD10 + N=N+10 + K(I)=N + IF(K(I).GT.IDIMT)K(I)=K(I)-IDIMT +20 CONTINUE +C need to find end of this line + IADD=LENGTH + IF((IDONE+IADD).GT.ITOT)IADD=ITOT-IDONE + IE=IS+IADD-1 + LD10M=MIN(LD10,(IE-IS+1)/10) + IDONE=IDONE+IADD +1001 FORMAT(' ',(12(5X,I6))) + WRITE(IDEV,1002) + +(SEQNCE(LWRAP(IDIMT,M)),M=IS-KSTART+1,IE-KSTART+1) + IF(NSTRND.EQ.1)WRITE(IDEV,1002)(SCOMP( + 1(SEQNCE(LWRAP(IDIMT,M)))),M=IS-KSTART+1,IE-KSTART+1) + WRITE(IDEV,1001)(K(I),I=1,LD10M) +1002 FORMAT( ' ',12(10A1,1X)) + IS=IE+1 + WRITE(IDEV,1005) +1005 FORMAT(' ') + GO TO 50 + END + SUBROUTINE LSTSQD(IDIM,KBIN,KBOUT,J1,J2,IDIMT,NSTRND, + +IHELPS,IHELPE,HELPF,IDEVH,I1,I2,LENGTH,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + INTEGER VALUE + IOK = 1 +1 CONTINUE + VALUE = I1 + CALL GETINT(I1,I2,VALUE, + +'List from ', + +VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0)RETURN + I1 = VALUE + VALUE = I2 + CALL GETINT(1,I2,VALUE, + +'List to ', + +VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0)RETURN + I2 = VALUE +C IS WRAP AROUND ALLOWED? + IF(I2.LT.I1)THEN + IF((J1.NE.1).OR.(J2.NE.IDIMT))THEN + WRITE(KBOUT,1004) +1004 FORMAT(' A sequence of this length cannot be treated as a', + + ' circle') + GO TO 1 + END IF + END IF + IOP = NSTRND + CALL YESONO(IOP,'Single stranded','Double stranded', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) RETURN + NSTRND = IOP + MININ = 10 + MAXIN = 120 + LENGTH = 60 + CALL GETINT(MININ,MAXIN,LENGTH, + +'Line length', + +VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0)RETURN + IF(MOD(VALUE,10).NE.0)VALUE = LENGTH + LENGTH = VALUE + END + SUBROUTINE COMPN(SEQ,IDIM1P,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,SCORES(5),DIALOG + CALL SHOWFU(KBOUT,'Plot base composition') + SCORES(5) = 0 + CALL GETPAR(24,10,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +SCORES(1),SCORES(2),SCORES(3),SCORES(4), + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + MAXSP = MIN(MXSPAN,MAXSP,J2-J1+1) + IF(DIALOG.EQ.1) THEN + CALL COMPD(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + + SCORES,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL COMPP(SEQ,IDIM1P,MXSPAN,SPAN,IWRIT,SCORES, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2) + CALL VT100M + END + SUBROUTINE COMPD(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +SCORES,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER HELPF*(*) + INTEGER SCORES(5) + CALL CHECK4('T','C','A','G', + +SCORES(1),SCORES(2),SCORES(3),SCORES(4), + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + END + SUBROUTINE COMPP(SEQ,IDIM1P,MXSPAN,SPAN,IWRIT,SCORES, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,SCORES(5),CTONUM + EXTERNAL CTONUM +C length forward and back + LF=SPAN/2 + LB=1+SPAN/2 + SCRMAX=1. + I1INM1=1+MXSPAN/2 + J1P=J1+I1INM1 + J2P=J2+I1INM1 + XMIN=J1 + XMAX=J2 +C set ymax + YMAX=SPAN*SCRMAX + YMIN=0. +C do edge + CALL START(SEQ(J1P-LB),IDIM1P,SCORES,5,SPAN,SUM) + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C do rest + IDONE=0 + XF=J1 + YF=SUM + DO 100 I=J1P,J2P + IDONE=IDONE+1 + SUM=SUM+SCORES(CTONUM(SEQ(I+LF))) + + -SCORES(CTONUM(SEQ(I-LB))) + IF(MOD(I,IWRIT).EQ.0)THEN + XT=I-I1INM1 + CALL LINE(XF,XT,YF,SUM,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XF=XT + YF=SUM + END IF +100 CONTINUE + END + SUBROUTINE START(SEQ,IDIM,SCORES,IDIMS,LENW,SUM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + INTEGER SCORES(IDIMS),CTONUM + EXTERNAL CTONUM + SUM=0. + DO 10 I=1,LENW + SUM=SUM+SCORES(CTONUM(SEQ(I))) +10 CONTINUE + END + SUBROUTINE CCHI(SEQ,IDIM1P,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,WORK,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + PARAMETER (ISIZE = 5) + INTEGER SPAN,DIALOG + REAL EXPEC(ISIZE),AVER(ISIZE),OBS(ISIZE),WORK(IDIM1P) +C NOTE J1 J2 IS THE ACTIVE REGION, KSTART IS THE NUMBER OF THE +C BASE IN ELEMENT 1 OF SEQ, AND IDIM1 IS THE LENGTH FROM KSTART +C TO THE END OF SEQ. PLOTS ARE FROM J1 TO J2 BUT STANDARDS CAN +C BE TAKEN FROM KSTART TO KSTART+IDIM1-1 = IENDB +C CHANGED TO ALLOW USER TO DEFINE REGION FOR STANDARD +C AND TO DO SCALING ONLY OVER REAL SEQUENCE (NOT EDGE) 23-08-84 + CALL SHOWFU(KBOUT, + +'Plot base composition differences as chi squared') + CALL GETPAR(25,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + K1 = KSTART + K2 = IENDB + IF(DIALOG.EQ.1) THEN + CALL CHID(KSTART,IENDB,MINSP,MAXSP,SPAN, + + MINIW,MAXIW,IWRIT,K1,K2, + + KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL CCHIP1(SEQ,IDIM1P,MXSPAN,EXPEC,OBS,AVER,ISIZE, + +SPAN,IWRIT,K1,K2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBOUT,WORK) + END + SUBROUTINE CCHIP1(SEQ,IDIM1P,MXSPAN,EXPEC,OBS,AVER,ISIZE, + +SPAN,IWRIT,K1,K2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBOUT,WORK) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,CTONUM + REAL EXPEC(ISIZE),AVER(ISIZE),OBS(ISIZE),WORK(IDIM1P) + EXTERNAL CTONUM,CIE + DIF = K2 - K1 + 1 + IF(DIF.LT.1.) RETURN + CALL FILLR(AVER,ISIZE,0.) + I1INM1= 1 + (MXSPAN/2)-KSTART+1 + CALL CHI1(SEQ(I1INM1+1),IDIM1P,K1,K2,AVER) + DO 3 I=1,ISIZE + AVER(I)=AVER(I) / DIF +3 CONTINUE +C length forward and back + LF=SPAN/2 + LB=1+SPAN/2 + J1P=J1+I1INM1 + J2P=J2+I1INM1 +C calc expected scores + DO 29 I=1,ISIZE + EXPEC(I)=AVER(I)*SPAN +29 CONTINUE + CALL BUSY(KBOUT) +C do edge + CALL FILLR(OBS,ISIZE,0.) + CALL CHI1(SEQ,IDIM1P,J1P,J1P+SPAN-1,OBS) +C do rest + SUMMAX=-99999. + SUMMIN=999999. + J = 1 + WORK(J) = CIE(OBS,EXPEC) + DO 200 I=J1P+LB,J2P-LF + ISF=CTONUM(SEQ(I+LF)) + ISB=CTONUM(SEQ(I-LB)) + OBS(ISF)=OBS(ISF)+1. + OBS(ISB)=OBS(ISB)-1. + SUM = CIE(OBS,EXPEC) + SUMMAX = MAX(SUMMAX,SUM) + SUMMIN = MIN(SUMMIN,SUM) + J = J + 1 + WORK(J) = SUM +200 CONTINUE + CALL CLEARV + CALL VECTOM + IDIMW = J2 - J1 + 1 - SPAN + XMIN = -LB + XMAX = IDIMW + LB + YMAX = SUMMAX + YMIN = SUMMIN + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL PLOTR(WORK,IDIMW,XMAX,XMIN,YMAX,YMIN,IWRIT, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL VT100M + WRITE(KBOUT,4444)SUMMAX,SUMMIN +4444 FORMAT(' Observed range=',2F12.6) + END + REAL FUNCTION CIE(OBS,EXPEC) + REAL OBS(4),EXPEC(4) + SUM = 0. + DO 10 K = 1,4 + TE = EXPEC(K) + IF(TE.EQ.0.0) GO TO 10 + TO = OBS(K) + D = TO - TE + SUM = SUM + D*D/TE +10 CONTINUE + CIE = SUM + END + SUBROUTINE PLOTR(POINTS,IDIMP,XMAX,XMIN,YMAX,YMIN,IWRIT, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + REAL POINTS(IDIMP) + XF = XMIN + YF = POINTS(1) + DO 100 I=1,IDIMP,IWRIT + XT = I + YT = POINTS(I) + CALL LINE(XF,XT,YF,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XF = XT + YF = YT +100 CONTINUE + END + SUBROUTINE CHI1(SEQ,IDIM1P,J1P,J2P,OBS) + REAL OBS(5) + CHARACTER SEQ(IDIM1P) + INTEGER CTONUM + EXTERNAL CTONUM + DO 10 I=J1P,J2P + IS = CTONUM(SEQ(I)) + OBS(IS) = OBS(IS) + 1. +10 CONTINUE + END + SUBROUTINE DICHI(SEQ,IDIM1P,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,WORK,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + PARAMETER (ISIZE = 25) + INTEGER SPAN,DIALOG + REAL EXPEC1(ISIZE),AVER1(ISIZE),OBS1(ISIZE) + REAL EXPEC(5,5),AVER(5,5),OBS(5,5),WORK(IDIM1P) + EQUIVALENCE (EXPEC1,EXPEC),(AVER1,AVER),(OBS1,OBS) +C NOTE J1 J2 IS THE ACTIVE REGION, KSTART IS THE NUMBER OF THE +C BASE IN ELEMENT 1 OF SEQ, AND IDIM1 IS THE LENGTH FROM KSTART +C TO THE END OF SEQ. PLOTS ARE FROM J1 TO J2 BUT STANDARDS CAN +C BE TAKEN FROM KSTART TO KSTART+IDIM1-1 = IENDB + CALL SHOWFU(KBOUT, + +'Plot dinucleotide composition differences as chi squared') +C CHANGED TO ALLOW USER TO DEFINE REGION FOR STANDARD +C AND TO DO SCALING ONLY OVER REAL SEQUENCE (NOT EDGE) 23-08-84 + CALL GETPAR(26,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + K1 = KSTART + K2 = IENDB + IF(DIALOG.EQ.1) THEN + CALL CHID(KSTART,IENDB,MINSP,MAXSP,SPAN, + + MINIW,MAXIW,IWRIT,K1,K2, + + KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL DCHIP1(SEQ,IDIM1P,MXSPAN,EXPEC,OBS,AVER,ISIZE, + +EXPEC1,OBS1,AVER1, + +SPAN,IWRIT,K1,K2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBOUT,WORK) + END + SUBROUTINE DCHI1(SEQ,IDIM1P,J1P,J2P,OBS) + REAL OBS(5,5) + CHARACTER SEQ(IDIM1P) + INTEGER CTONUM + EXTERNAL CTONUM + DO 10 I=J1P,J2P + IS = CTONUM(SEQ(I)) + IS1 = CTONUM(SEQ(I+1)) + OBS(IS,IS1) = OBS(IS,IS1) + 1. +10 CONTINUE + END + SUBROUTINE DCHIP1(SEQ,IDIM1P,MXSPAN,EXPEC,OBS,AVER,ISIZE, + +EXPEC1,OBS1,AVER1, + +SPAN,IWRIT,K1,K2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBOUT,WORK) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,CTONUM + REAL EXPEC1(ISIZE),AVER1(ISIZE),OBS1(ISIZE) + REAL EXPEC(5,5),AVER(5,5),OBS(5,5),WORK(IDIM1P) + EXTERNAL CTONUM,CED + DIF = K2 - K1 + IF(DIF.LT.2.) RETURN + CALL BUSY(KBOUT) + CALL FILLR(AVER1,ISIZE,0.) + I1INM1=1+(MXSPAN/2)-KSTART+1 + CALL DCHI1(SEQ(I1INM1+1),IDIM1P,K1,K2-1,AVER) + DO 3 I=1,5 + DO 3 J=1,5 + AVER(I,J)=AVER(I,J) / DIF +3 CONTINUE +C length forward and back + LF=SPAN/2 + LB=1+SPAN/2 + J1P=J1+I1INM1 + J2P=J2+I1INM1 +C calc expected scores + DO 29 I=1,5 + DO 29 J=1,5 + EXPEC(I,J)=AVER(I,J)*SPAN +29 CONTINUE +C set ymax +C do edge + CALL FILLR(OBS1,ISIZE,0.) + CALL DCHI1(SEQ,IDIM1P,J1P,J1P+SPAN-1,OBS) + J = 1 + WORK(J) = CED(OBS,EXPEC) +C do rest + SUMMAX=-999999. + SUMMIN=9999999. + DO 200 I=J1P+LB,J2P-1-LF + ISF=CTONUM(SEQ(I+LF)) + ISF1=CTONUM(SEQ(I+LF+1)) + ISB=CTONUM(SEQ(I-LB)) + ISB1=CTONUM(SEQ(I-LB+1)) + OBS(ISF,ISF1)=OBS(ISF,ISF1)+1. + OBS(ISB,ISB1)=OBS(ISB,ISB1)-1. + SUM = CED(OBS,EXPEC) + J = J + 1 + WORK(J) = SUM + SUMMAX=MAX(SUMMAX,SUM) + SUMMIN=MIN(SUMMIN,SUM) +200 CONTINUE + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + IDIMW = J2 - J1 - SPAN + XMIN = -LB + XMAX = IDIMW + LB + YMAX = SUMMAX + YMIN = SUMMIN + CALL PLOTR(WORK,IDIMW,XMAX,XMIN,YMAX,YMIN,IWRIT, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL VT100M + WRITE(KBOUT,4444)SUMMAX,SUMMIN +4444 FORMAT(' Observed range=',2F12.6) + RETURN + END + REAL FUNCTION CED(OBS,EXPEC) + REAL OBS(5,5),EXPEC(5,5) + SUM = 0. + DO 10 K=1,4 + DO 10 J=1,4 + TE = EXPEC(K,J) + IF(TE.EQ.0.) GO TO 10 + TO = OBS(K,J) + D = TO - TE + SUM = SUM + D*D/TE +10 CONTINUE + CED = SUM + END + SUBROUTINE TRICHI(SEQ,IDIM1P,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,WORK,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + PARAMETER (ISIZE = 125) + INTEGER SPAN,DIALOG + REAL EXPEC1(ISIZE),AVER1(ISIZE),OBS1(ISIZE) + REAL EXPEC(5,5,5),AVER(5,5,5),OBS(5,5,5),WORK(IDIM1P) + EQUIVALENCE (EXPEC1,EXPEC),(AVER1,AVER),(OBS1,OBS) +C NOTE J1 J2 IS THE ACTIVE REGION, KSTART IS THE NUMBER OF THE +C BASE IN ELEMENT 1 OF SEQ, AND IDIM1 IS THE LENGTH FROM KSTART +C TO THE END OF SEQ. PLOTS ARE FROM J1 TO J2 BUT STANDARDS CAN +C BE TAKEN FROM KSTART TO KSTART+IDIM1-1 = IENDB + CALL SHOWFU(KBOUT, + +'Plot trinucleotide composition differences as chi squared') +C CHANGED TO ALLOW USER TO DEFINE REGION FOR STANDARD +C AND TO DO SCALING ONLY OVER REAL SEQUENCE (NOT EDGE) 23-08-84 + CALL GETPAR(27,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + K1 = KSTART + K2 = IENDB + IF(DIALOG.EQ.1) THEN + CALL CHID(KSTART,IENDB,MINSP,MAXSP,SPAN, + + MINIW,MAXIW,IWRIT,K1,K2, + + KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL TCHIP1(SEQ,IDIM1P,MXSPAN,EXPEC,OBS,AVER,ISIZE, + +EXPEC1,OBS1,AVER1, + +SPAN,IWRIT,K1,K2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBOUT,WORK) + END + SUBROUTINE TCHIP1(SEQ,IDIM1P,MXSPAN,EXPEC,OBS,AVER,ISIZE, + +EXPEC1,OBS1,AVER1, + +SPAN,IWRIT,K1,K2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBOUT,WORK) + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,CTONUM + REAL EXPEC1(ISIZE),AVER1(ISIZE),OBS1(ISIZE) + REAL EXPEC(5,5,5),AVER(5,5,5),OBS(5,5,5),WORK(IDIM1P) + EXTERNAL CTONUM,CET + DIF = K2 - K1 - 2 + IF(DIF.LT.3.) RETURN + CALL BUSY(KBOUT) + CALL FILLR(AVER1,ISIZE,0.) + I1INM1 = 1 + (MXSPAN/2) - KSTART + 1 + CALL TCHI1(SEQ(I1INM1+1),IDIM1P,K1,K2-2,AVER) + DO 3 I=1,5 + DO 3 J=1,5 + DO 3 L=1,5 + AVER(I,J,L)=AVER(I,J,L) / DIF +3 CONTINUE +C length forward and back + LF=SPAN/2 + LB=1+SPAN/2 + J1P=J1+I1INM1 + J2P=J2+I1INM1 +C calc expected scores + DO 29 I=1,5 + DO 29 J=1,5 + DO 29 L=1,5 + EXPEC(I,J,L)=AVER(I,J,L)*SPAN +29 CONTINUE +C do edge + CALL FILLR(OBS1,ISIZE,0.) + CALL TCHI1(SEQ,IDIM1P,J1P,J1P+SPAN-1,OBS) + J = 1 + WORK(J) = CET(OBS,EXPEC) +C do rest + SUMMAX=-99999. + SUMMIN=999999. + DO 200 I=J1P+LB,J2P-2-LF + IS=CTONUM(SEQ(I+LF)) + IS1=CTONUM(SEQ(I+LF+1)) + IS2=CTONUM(SEQ(I+LF+2)) + IB=CTONUM(SEQ(I-LB)) + IB1=CTONUM(SEQ(I-LB+1)) + IB2=CTONUM(SEQ(I-LB+2)) + OBS(IS,IS1,IS2)=OBS(IS,IS1,IS2)+1. + OBS(IB,IB1,IB2)=OBS(IB,IB1,IB2)-1. + SUM = CET(OBS,EXPEC) + J = J + 1 + WORK(J) = SUM + SUMMAX=MAX(SUMMAX,SUM) + SUMMIN=MIN(SUMMIN,SUM) +200 CONTINUE + CALL FILLR(OBS1,ISIZE,0.) + CALL TCHI1(SEQ,IDIM1P,J1P,J1P+SPAN-1,OBS) + J = 1 + WORK(J) = CET(OBS,EXPEC) + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + IDIMW = J2 - J1 - 1 - SPAN + XMIN = -LB + XMAX = IDIMW + LB + YMAX = SUMMAX + YMIN = SUMMIN + CALL PLOTR(WORK,IDIMW,XMAX,XMIN,YMAX,YMIN,IWRIT, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL VT100M + WRITE(KBOUT,4444)SUMMAX,SUMMIN +4444 FORMAT(' Observed range=',2F12.6) + RETURN + END + REAL FUNCTION CET(OBS,EXPEC) + REAL OBS(5,5,5),EXPEC(5,5,5) + SUM=0. + DO 10 K=1,4 + DO 10 J=1,4 + DO 10 L=1,4 + TEXP=EXPEC(K,J,L) + IF(TEXP.EQ.0.)GO TO 10 + TOBS=OBS(K,J,L) + OME=TOBS-TEXP + SUM=SUM+(OME*OME)/TEXP +10 CONTINUE + CET = SUM + END + SUBROUTINE TCHI1(SEQ,IDIM1P,J1P,J2P,OBS) + CHARACTER SEQ(IDIM1P) + REAL OBS(5,5,5) + INTEGER CTONUM + EXTERNAL CTONUM + DO 10 I=J1P,J2P + IS=CTONUM(SEQ(I)) + IS1=CTONUM(SEQ(I+1)) + IS2=CTONUM(SEQ(I+2)) + OBS(IS,IS1,IS2)=OBS(IS,IS1,IS2)+1. +10 CONTINUE + END + SUBROUTINE NEGP1(SEQ,IDIM1P,MXSPAN,OBS,AVER, + +SPAN,IWRIT,K1,K2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBOUT,WORK) + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,OBS(5),CTONUM + REAL AVER(5),WORK(IDIM1P) + REAL NEI + EXTERNAL CTONUM,NEI + I1INM1=1+(MXSPAN/2)-KSTART+1 + J1P=J1+I1INM1 + J2P=J2+I1INM1 + DIF = K2 - K1 + 1 + IF(DIF.LT.1.) RETURN + CALL FILLR(AVER,5,0.) + CALL NEG1(SEQ(I1INM1+1),IDIM1P,K1,K2,AVER) + DIF = DIF * SPAN + DO 3 I=1,5 + AVER(I)=AVER(I) / DIF +3 CONTINUE +C length forward and back + LF=SPAN/2 + LB=1+SPAN/2 + CALL BUSY(KBOUT) +C calc expected scores +C do edge + CALL FILLI(OBS,5,0) + DO 166 I=J1P,J1P+SPAN-1 + ISF=CTONUM(SEQ(I+LF)) + ISB=CTONUM(SEQ(I-LB)) + OBS(ISF)=OBS(ISF)+1 + OBS(ISB)=OBS(ISB)-1 +166 CONTINUE + SUMMAX=-99999. + SUMMIN=999999. + WIND=SPAN + J = 1 + WORK(J) = NEI(OBS,AVER,WIND) + DO 200 I=J1P+LB,J2P-LF + ISF=CTONUM(SEQ(I+LF)) + ISB=CTONUM(SEQ(I-LB)) + OBS(ISF)=OBS(ISF)+1 + OBS(ISB)=OBS(ISB)-1 + SUM = NEI(OBS,AVER,WIND) + J = J + 1 + WORK(J) = SUM + SUMMAX=MAX(SUMMAX,SUM) + SUMMIN=MIN(SUMMIN,SUM) +200 CONTINUE +C set ymax + CALL CLEARV + CALL VECTOM + IDIMW = J2 - J1 + 1 - SPAN + XMIN = -LB + XMAX = IDIMW + LB + YMAX = SUMMAX + YMIN = SUMMIN + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL PLOTR(WORK,IDIMW,XMAX,XMIN,YMAX,YMIN,IWRIT, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL VT100M + WRITE(KBOUT,4444)SUMMAX,SUMMIN +4444 FORMAT(' Observed range=',2F12.6) + END + REAL FUNCTION NEI(OBS,AVER,WIND) + REAL AVER(5) + INTEGER OBS(5) + SUM=0. + DO 10 K=1,4 + TEMP=REAL(OBS(K))*AVER(K) + IF(TEMP.GT.0.) TEMP1 = TEMP*LOG(TEMP) + SUM=SUM-TEMP1 +10 CONTINUE + SUM=SUM/WIND + NEI = SUM + END + SUBROUTINE NEG1(SEQ,IDIM1P,K1,K2,AVER) + CHARACTER SEQ(IDIM1P) + REAL AVER(5) + INTEGER CTONUM + EXTERNAL CTONUM + DO 2 I=K1,K2 + IS=CTONUM(SEQ(I)) + AVER(IS)=AVER(IS)+1. +2 CONTINUE + END + SUBROUTINE NEGENT(SEQ,IDIM1P,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,WORK,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + PARAMETER (ISIZE = 5) + INTEGER SPAN,DIALOG + REAL AVER(ISIZE),WORK(IDIM1P) + INTEGER OBS(ISIZE) +C NOTE J1 J2 IS THE ACTIVE REGION, KSTART IS THE NUMBER OF THE +C BASE IN ELEMENT 1 OF SEQ, AND IDIM1 IS THE LENGTH FROM KSTART +C TO THE END OF SEQ. PLOTS ARE FROM J1 TO J2 BUT STANDARDS CAN +C BE TAKEN FROM KSTART TO KSTART+IDIM1-1 = IENDB +C CHANGED TO ALLOW USER TO DEFINE REGION FOR STANDARD +C AND TO DO SCALING ONLY OVER REAL SEQUENCE (NOT EDGE) 23-08-84 + CALL SHOWFU(KBOUT,'Plot negentropy') + CALL GETPAR(59,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + K1 = KSTART + K2 = IENDB + IF(DIALOG.EQ.1) THEN + CALL CHID(KSTART,IENDB,MINSP,MAXSP,SPAN, + + MINIW,MAXIW,IWRIT,K1,K2, + + KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL NEGP1(SEQ,IDIM1P,MXSPAN,OBS,AVER, + +SPAN,IWRIT,K1,K2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBOUT,WORK) + END + SUBROUTINE CHID(KSTART,IENDB,MINSP,MAXSP,SPAN, + +MINIW,MAXIW,IWRIT,K1,K2, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + INTEGER SPAN + IOK = 1 + CALL GTREG(KBIN,KBOUT,KSTART,IENDB,K1,K2, + +'Define region for standard', + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C IF(IOK.NE.0) RETURN + RETURN + END + SUBROUTINE ZDNA(SEQ,IDIM1P,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,SCORE(5,5),CTONUM,DIALOG + REAL OBS(5,5) + EXTERNAL CTONUM + CALL SHOWFU(KBOUT,'Plot z dna potential') + CALL GETPAR(33,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(DIALOG.EQ.1) THEN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + I1INM1=1+MXSPAN/2 + DO 1 I=1,5 + DO 1 J=1,5 + SCORE(I,J)=0 +1 CONTINUE +C at + SCORE(3,1)=1 + SCORE(1,3)=1 +C cg + SCORE(2,4)=1 + SCORE(4,2)=1 +C gt + SCORE(4,1)=1 + SCORE(1,4)=1 +C ac + SCORE(3,2)=1 + SCORE(2,3)=1 + J1P=J1+I1INM1 + J2P=J2+I1INM1 + XMIN=J1 + XMAX=J2 +C length forward and back + LF=SPAN/2 + LB=1+SPAN/2 +C calc expected scores + YMAX=SPAN + YMIN=0. +C do edge + DO 65 I=1,5 + DO 65 J=1,5 + OBS(I,J)=0. +65 CONTINUE + DO 66 I=J1P-LB,J1P-LB+SPAN-1 + IS=CTONUM(SEQ(I)) + IS1=CTONUM(SEQ(I+1)) + OBS(IS,IS1)=OBS(IS,IS1)+1. +66 CONTINUE + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C do rest + XF=J1 + YF=SUM + DO 100 I=J1P,J2P-1 + ILF=CTONUM(SEQ(I+LF)) + ILF1=CTONUM(SEQ(I+LF+1)) + ILB=CTONUM(SEQ(I-LB)) + ILB1=CTONUM(SEQ(I-LB+1)) + OBS(ILF,ILF1)=OBS(ILF,ILF1)+1. + OBS(ILB,ILB1)=OBS(ILB,ILB1)-1. + IF(MOD(I,IWRIT).EQ.0)THEN + SUM=0. + DO 99 K=1,4 + DO 99 J=1,4 + SUM=SUM+SCORE(K,J)*OBS(K,J) +99 CONTINUE + XT=I-I1INM1 + CALL LINE(XF,XT,YF,SUM,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XF=XT + YF=SUM + END IF +100 CONTINUE + CALL VT100M + END + SUBROUTINE ZDNARD(SEQ,IDIM1P,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,RVAL(5),YVAL(5),CTONUM,DIALOG + EXTERNAL CTONUM + SAVE RVAL,YVAL + DATA RVAL/0,0,1,1,0/,YVAL/1,1,0,0,0/ + CALL SHOWFU(KBOUT,'Plot z dna potential') + CALL GETPAR(35,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(DIALOG.EQ.1) THEN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + I1INM1=1+MXSPAN/2 + J1P=J1+I1INM1 + J2P=J2+I1INM1 + XMIN=J1 + XMAX=J2 +C length forward and back + LF=SPAN/2 + LB=1+SPAN/2 +C calc expected scores + YMAX=SPAN+1 + YMIN=LF+1 +C do edge + RSUM1=0. + YSUM1=0. + RSUM2=0. + YSUM2=0. + DO 66 I=J1P-LB,J1P-LB+SPAN-1,2 + IS=CTONUM(SEQ(I)) + IS1=CTONUM(SEQ(I+1)) + RSUM1=RSUM1+RVAL(IS) + YSUM1=YSUM1+YVAL(IS1) + RSUM2=RSUM2+RVAL(IS1) + YSUM2=YSUM2+YVAL(IS) +66 CONTINUE + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C do rest + XF=J1 + YF=SUM + DO 100 I=J1P,J2P-1,2 + ILB=CTONUM(SEQ(I-LB)) + ILB1=CTONUM(SEQ(I-LB+1)) + ILF1=CTONUM(SEQ(I+LF+1)) + ILF2=CTONUM(SEQ(I+LF+2)) + RSUM1=RSUM1-RVAL(ILB)+RVAL(ILF1) + YSUM1=YSUM1-YVAL(ILB1)+YVAL(ILF2) + RSUM2=RSUM2-RVAL(ILB1)+RVAL(ILF2) + YSUM2=YSUM2-YVAL(ILB)+YVAL(ILF1) + IF(MOD(I,IWRIT).EQ.0)THEN + SUM=MAX((RSUM1+YSUM1),(RSUM2+YSUM2)) + XT=I-I1INM1 + CALL LINE(XF,XT,YF,SUM,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XF=XT + YF=SUM + END IF +100 CONTINUE + CALL VT100M + END + SUBROUTINE ZDNARN(SEQ,IDIM1, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1) + INTEGER SPAN,CTONUM,DIALOG + EXTERNAL CTONUM + CALL SHOWFU(KBOUT,'Plot z dna potential') + CALL GETPAR(34,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(DIALOG.EQ.1) THEN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + XMIN=J1 + XMAX=J2 + YMAX=SPAN + YMIN=0. + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C do rest + XF=J1 + YF=0. + I=J1-1 +100 CONTINUE +C + I=I+1 + IF(I.GT.J2)GO TO 500 + IS=CTONUM(SEQ(I)) + IF(IS.EQ.5)GO TO 100 + SUM=0. + IF(IS.LT.3)GO TO 300 +C must be a or g +200 CONTINUE +C want c or t + I=I+1 + IF(I.GT.J2)GO TO 500 + IS=CTONUM(SEQ(I)) + IF(IS.EQ.5)GO TO 100 + IF(IS.GT.2)GO TO 100 +C have c or t + SUM=SUM+1. + IF(MOD(I,IWRIT).EQ.0)THEN + XT=I + CALL LINE(XF,XT,YF,SUM,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XF=XT + YF=SUM + END IF +C now want a or g +300 CONTINUE + I=I+1 + IF(I.GT.J2)GO TO 500 + IS=CTONUM(SEQ(I)) + IF(IS.EQ.5)GO TO 100 + IF(IS.LT.3)GO TO 100 +C have a or g + SUM=SUM+1. + IF(MOD(I,IWRIT).EQ.0)THEN + XT=I + CALL LINE(XF,XT,YF,SUM,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XF=XT + YF=SUM + END IF +C want c or t + GO TO 200 +500 CONTINUE + CALL VT100M + END + SUBROUTINE GETRC1(KBIN,KBOUT,J1,J2,IDIMT,I1,I2,IDIM1, + +P, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*),P*(*) + INTEGER VALUE +C VERSION FOR LIST REGION + IOK = 1 + WRITE(KBOUT,1000)P +1000 FORMAT(' ',A) +1 CONTINUE + VALUE = J1 + I1 = J1 + I2 = J2 + CALL GETINT(I1,I2,VALUE, + +'From', + +VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0)RETURN + I1 = VALUE + VALUE = I2 + K1 = MIN(1,J1) + CALL GETINT(K1,J2,VALUE, + +'To', + +VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0)RETURN + I2 = VALUE +C IS WRAP AROUND ALLOWED? + IF(I2.LT.I1)THEN + IF((J1.NE.1).OR.(J2.NE.IDIMT))THEN + WRITE(KBOUT,1001) +1001 FORMAT(' a sequence of this length cannot be treated as a', + + ' circle') + GO TO 1 + END IF + I2 = I2 + IDIM1 + END IF + END + SUBROUTINE GETRC2(KBIN,KBOUT,J1,J2,IDIMT,I1,I2, + +P, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*),P*(*) + INTEGER VALUE +C VERSION FOR TRANSLATE REGION + IOK = 1 + WRITE(KBOUT,1000)P +1000 FORMAT(' ',A) +1 CONTINUE + VALUE = 0 + I1 = 0 + I2 = J2 + CALL GETINT(I1,I2,VALUE, + +'From', + +VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0)RETURN + I1 = VALUE + IF(I1.EQ.0) THEN + IOK = 0 + RETURN + END IF + VALUE = J2 + I2 = J2 + CALL GETINT(1,I2,VALUE, + +'To', + +VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0)RETURN + I2 = VALUE +C IS WRAP AROUND ALLOWED? + IF(I2.LT.I1)THEN + IF((J1.NE.1).OR.(J2.NE.IDIMT))THEN + WRITE(KBOUT,1001) +1001 FORMAT(' A sequence of this length cannot be treated as a', + + ' circle') + GO TO 1 + END IF + END IF + END + SUBROUTINE FMTRAN(SEQ,IDIM1,IS,IE,OUTP,IDMP,LENGTH,IDEV, + + KSTART,IDIMT,CODE) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),OUTP(IDMP,3),BLANK + INTEGER IO1(12),CODE + CHARACTER IT,OUTLIN(132),THREEL*3,THREE*3 + EXTERNAL LWRAP,THREEL + SAVE BLANK + DATA BLANK/' '/ + LD10 = LENGTH/10 + ITOT = IE - IS + 1 + IF(IE.LE.IS)ITOT = IDIMT - ITOT + IDONE = 0 +C SET POINTER TO ELEMENT NUMBERS (TREATING ARRAY AS LINEAR) TO +C START POINTER + IBEG = IS - KSTART + 1 + I1 = IS - 1 + WRITE(IDEV,1000) +50 CONTINUE + IF(IDONE.GE.ITOT)RETURN +C NEED TO FIND END OF THIS LINE AND ADD TO COUNT + IADD = LENGTH + IF((IDONE + IADD).GT.ITOT)IADD = ITOT - IDONE + IEND = IBEG + IADD - 1 + IDONE = IDONE + IADD + LD10M = MIN(LD10,(IEND - IBEG + 1)/10) + DO 200 J = 1,3 + IBEGP = IBEG + J - 1 + IENDP = IEND + J - 1 +C NUMBER OF CODONS THIS LINE? + NP = (IENDP - IBEGP + 1)/3 +C TEST FOR NO CODONS + IF(NP.GT.0)THEN +C THIS LINE STARTS AT IBEG AND ENDS AT IEND (TREATING ARRAY AS +C LINEAR) NEED TO KNOW IF THERE ARE ANY AA'S TO OUTPUT. +C TRANSLATE POINTERS AND TEST THEM +C FILL OUTP WITH BLANKS + CALL FILLC(OUTLIN,132,' ') + IEMPT = 0 + ITMPCS = IBEGP + L = J - 2 + DO 100 I = 1,NP + IOUT = LWRAP(IDIM1,ITMPCS)/3 + 1 + ITMPCS = ITMPCS + 3 + IT = OUTP(IOUT,J) + IF(IT.NE.BLANK)IEMPT = 1 + L = L + 3 + IF(CODE.EQ.3)THEN + THREE = THREEL(IT) + OUTLIN(L - 1) = THREE(1:1) + OUTLIN(L) = THREE(2:2) + OUTLIN(L + 1) = THREE(3:3) + ELSE + OUTLIN(L) = IT + END IF +100 CONTINUE + IF(IEMPT.GT.0) + + WRITE(IDEV,1002)(OUTLIN(K),K = 1,LENGTH + 6) + END IF +200 CONTINUE + WRITE(IDEV,1002)(SEQ(LWRAP(IDIM1,K)),K = IBEG,IEND) +1002 FORMAT(' ',132A1) + DO 300 K = 1,LD10 + I1 = I1 + 10 + IO1(K) = I1 + IF(IO1(K).GT.IDIMT)IO1(K) = IO1(K) - IDIMT +300 CONTINUE + WRITE(IDEV,1001)(IO1(K),K = 1,LD10M) +1001 FORMAT( ' ',12(4X,I6)) + IBEG = IEND + 1 + WRITE(IDEV,1000) +1000 FORMAT(/) + GO TO 50 + END + SUBROUTINE TRAND4(MINO,MAXO,MINOPN,JSTRAN,FILNAM,IDEV,IOK, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*),FILNAM*(*) + IOK = 1 + CALL GETINT(MINO,MAXO,MINOPN, + +'Minimum open frame in amino acids', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINOPN = IVAL + IVAL = JSTRAN + 1 + CALL GSTRND(IVAL,IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IVAL.LT.1) THEN + IOK = 1 + RETURN + END IF + JSTRAN = IVAL - 1 +30 CONTINUE + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,1,IOK,KBIN,KBOUT, + +'File name for translation', + +IHELPS,IHELPE,HELPF,IDEVH) + END + SUBROUTINE TRAND5(MINO,MAXO,MINOPN,JSTRAN,IOK, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + IOK = 1 + CALL GETINT(MINO,MAXO,MINOPN, + +'Minimum open frame in amino acids', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINOPN = IVAL + IVAL = JSTRAN + 1 + CALL GSTRND(IVAL,IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IVAL.LT.1) THEN + IOK = 1 + RETURN + END IF + JSTRAN = IVAL - 1 + END + SUBROUTINE TRNPIR(SEQ,IDIM,I1,I2,KSTART,KBIN,KBOUT,IDEV,PAA, + +OUTP,IDIMP,FILNAM,MINO,MAXO,MINOPN,JSTRAN, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIMP),PAA(125),OUTP(IDIMP) + CHARACTER FILNAM*(*),HELPF*(*) + CALL TRAND4(MINO,MAXO,MINOPN,JSTRAN,FILNAM,IDEV,IOK, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) THEN + CLOSE(UNIT=IDEV) + RETURN + END IF + MINOPD = 3 * MINOPN + IF(JSTRAN.EQ.0) THEN + CALL TRNDOP(SEQ,IDIM,I1,I2,OUTP,IDIMP,PAA,MINOPD,IDEV) + ELSE IF (JSTRAN.EQ.1) THEN + CALL TRNDOM(SEQ,IDIM,I1,I2,OUTP,IDIMP,PAA,MINOPD,IDEV) + ELSE IF (JSTRAN.EQ.2) THEN + CALL TRNDOP(SEQ,IDIM,I1,I2,OUTP,IDIMP,PAA,MINOPD,IDEV) + CALL TRNDOM(SEQ,IDIM,I1,I2,OUTP,IDIMP,PAA,MINOPD,IDEV) + END IF + CLOSE(UNIT=IDEV) + END + SUBROUTINE TRNDOP(SEQ,IDDNA,J1,J2, + +ACIDS,IDACID,PAA,MINOPN,IDEV) + CHARACTER SEQ(IDDNA),ACIDS(IDACID),PAA(5,5,5) + CHARACTER LINE*60 + INTEGER STEP(3),FRAME,PSTOP,WPAIR + EXTERNAL PSTOP,MINIL,WPAIR,ITOSL +C Find open reading frames in their order of occurrence +C For each of 3 frames remember next position to try + STEP(3) = J1 + 2 + STEP (1) = J1 + STEP(2) = J1 + 1 + 10 CONTINUE + FRAME = MINIL(STEP,3) + I = STEP(FRAME) + IF(I.LT.J2) THEN + J = PSTOP(SEQ,IDDNA,I,J2,ACIDS,IDACID,PAA,IACID) + STEP(FRAME) = J + 4 + IF((J-I+1).GE.MINOPN) THEN + LINE(1:) = '>' + IF (ITOSL(LINE(2:),I).EQ.0) WRITE(*,*)'Scream: ITOSL' + IF(WPAIR(LINE(22:),I,J).NE.0) WRITE(*,*)'Scream: WPAIR' + WRITE(IDEV,1001)LINE + IACID = IACID + 1 + ACIDS(IACID) = '*' + CALL FMTDKN(IDEV,ACIDS,IACID) + END IF + GO TO 10 + END IF + 1001 FORMAT(A) + END + SUBROUTINE TRNDOM(SEQ,IDDNA,J1,J2,ACIDS,IDACID,PAA,MINOPN,IDEV) + CHARACTER SEQ(IDDNA),ACIDS(IDACID),PAA(5,5,5),LINE*60 + INTEGER STEP(3),FRAME,MSTOP,WPAIR + EXTERNAL MSTOP,MINIL,WPAIR,ITOSL +C 17-7-91 replced line to write out title! which had disapeared +C Find open reading frames in their order of occurrence +C For each of 3 frames remember next position to try + STEP(3) = J1 + 2 + STEP (1) = J1 + STEP(2) = J1 + 1 + 10 CONTINUE + FRAME = MINIL(STEP,3) + I = STEP(FRAME) + IF(I.LT.J2) THEN + J = MSTOP(SEQ,IDDNA,I,J2,ACIDS,IDACID,PAA,IACID) + STEP(FRAME) = J + 4 + IF((J-I+1).GE.MINOPN) THEN + LINE(1:) = '> complement(' + IF (ITOSL(LINE(2:),I).EQ.0) WRITE(*,*)'Scream: ITOSL' + IF(WPAIR(LINE(33:),I,J).NE.0) WRITE(*,*)'Scream: WPAIR' + LINE(32+INDEX(LINE(33:),' '):) = ')' + WRITE(IDEV,1001)LINE + CALL SQREV(ACIDS,IACID) + IACID = IACID + 1 + ACIDS(IACID) = '*' + CALL FMTDKN(IDEV,ACIDS,IACID) + END IF + GO TO 10 + END IF + 1001 FORMAT(A) + END + SUBROUTINE OPENFR(SEQ,IDIM,I1,I2,KSTART,IDEV,KBIN,KBOUT,PAA, + +OUTP,IDIMP, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIMP),PAA(125),OUTP(IDIMP) + CHARACTER HELPF*(*) + CALL SHOWFU(KBOUT,'Find open reading frames') + CALL GETPAR(54,4,IOK,MINO,MAXO,MINOPN,JSTRAN, + +IPAR5, + +IPAR6,IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + CALL TRAND5(MINO,MAXO,MINOPN,JSTRAN,IOK, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + MINOPD = 3* MINOPN + IF(JSTRAN.EQ.0) THEN + CALL TRNDP(SEQ,IDIM,I1,I2,OUTP,IDIMP,PAA,MINOPD,IDEV) + ELSE IF (JSTRAN.EQ.1) THEN + CALL TRNDM(SEQ,IDIM,I1,I2,OUTP,IDIMP,PAA,MINOPD,IDEV) + ELSE IF (JSTRAN.EQ.2) THEN + CALL TRNDP(SEQ,IDIM,I1,I2,OUTP,IDIMP,PAA,MINOPD,IDEV) + CALL TRNDM(SEQ,IDIM,I1,I2,OUTP,IDIMP,PAA,MINOPD,IDEV) + END IF + END + SUBROUTINE TRNDP(SEQ,IDDNA,J1,J2, + +ACIDS,IDACID,PAA,MINOPN,IDEV) + CHARACTER SEQ(IDDNA),ACIDS(IDACID),PAA(5,5,5) + CHARACTER LINE*60 + INTEGER STEP(3),FRAME,PSTOP,WPAIR + EXTERNAL PSTOP,MINIL,WPAIR +C Find open reading frames in their order of occurrence +C For each of 3 frames remember next position to try + STEP(3) = J1 + 2 + STEP (1) = J1 + STEP(2) = J1 + 1 + 10 CONTINUE + FRAME = MINIL(STEP,3) + I = STEP(FRAME) + IF(I.LT.J2) THEN + J = PSTOP(SEQ,IDDNA,I,J2,ACIDS,IDACID,PAA,IACID) + STEP(FRAME) = J + 4 + IF((J-I+1).GE.MINOPN) THEN + LINE(1:) = 'FT CDS' + IF(WPAIR(LINE(22:),I,J).NE.0) WRITE(*,*)'Scream: WPAIR' + WRITE(IDEV,1001)LINE + END IF + GO TO 10 + END IF + 1001 FORMAT(A) + END + SUBROUTINE TRNDM(SEQ,IDDNA,J1,J2,ACIDS,IDACID,PAA,MINOPN,IDEV) + CHARACTER SEQ(IDDNA),ACIDS(IDACID),PAA(5,5,5),LINE*60 + INTEGER STEP(3),FRAME,MSTOP,WPAIR + EXTERNAL MSTOP,MINIL,WPAIR +C Find open reading frames in their order of occurrence +C For each of 3 frames remember next position to try + STEP(3) = J1 + 2 + STEP (1) = J1 + STEP(2) = J1 + 1 + 10 CONTINUE + FRAME = MINIL(STEP,3) + I = STEP(FRAME) + IF(I.LT.J2) THEN + J = MSTOP(SEQ,IDDNA,I,J2,ACIDS,IDACID,PAA,IACID) + STEP(FRAME) = J + 4 + IF((J-I+1).GE.MINOPN) THEN + LINE(1:) = 'FT CDS complement(' + IF(WPAIR(LINE(33:),I,J).NE.0) WRITE(*,*)'Scream: WPAIR' + LINE(32+INDEX(LINE(33:),' '):) = ')' + WRITE(IDEV,1001)LINE + END IF + GO TO 10 + END IF + 1001 FORMAT(A) + END + INTEGER FUNCTION PSTOP(SEQ,IDDNA,IDNA1,IDNA2, + +ACIDS,IDACID,PAA,IACID) + CHARACTER PAA(5,5,5),SEQ(IDDNA),ACIDS(IDACID),TRANF,ACID + EXTERNAL TRANF +C Returns position of next stop codon or IDNA2 if end reached +C Also the number of acids excluding any stop codon +C Also the acids + IACID = 0 + DO 10 IDNA=IDNA1,IDNA2-2,3 + ACID = TRANF(SEQ(IDNA),PAA) + IF(ACID.EQ.'*') THEN + PSTOP = IDNA - 1 + RETURN + ELSE + IACID = IACID + 1 + ACIDS(IACID) = ACID + END IF + 10 CONTINUE + PSTOP = IDNA2 + END + INTEGER FUNCTION MSTOP(SEQ,IDDNA,IDNA1,IDNA2, + +ACIDS,IDACID,PAA,IACID) + CHARACTER PAA(5,5,5),SEQ(IDDNA),ACIDS(IDACID),TRANB,ACID + EXTERNAL TRANB +C Returns position of next stop codon or IDNA2 if end reached +C Also the number of acids excluding any stop codon +C Also the acids + IACID = 0 + DO 10 IDNA=IDNA1,IDNA2-2,3 + ACID = TRANB(SEQ(IDNA),PAA) + IF(ACID.EQ.'*') THEN + MSTOP = IDNA - 1 + RETURN + ELSE + IACID = IACID + 1 + ACIDS(IACID) = ACID + END IF + 10 CONTINUE + MSTOP = IDNA2 + END + INTEGER FUNCTION MINIL(LIST,NLIST) + INTEGER LIST(NLIST) +C sent list, return element number of first element whose +C value equals the minimum value in the list. + MX = LIST(1) + DO 10 I=2,NLIST + MX = MIN(MX,LIST(I)) + 10 CONTINUE + DO 20 I=1,NLIST + IF(MX.EQ.LIST(I)) THEN + MINIL = I + RETURN + END IF + 20 CONTINUE +C Should never get here! + WRITE(*,*)'Scream: MINIL' + MINIL = 1 + END + INTEGER FUNCTION WPAIR(LINE,I,J) + CHARACTER LINE*(*) + EXTERNAL ITOSL +C puts a pair of integers into string line, as for a feature table + WPAIR = 1 + IF(ITOSL(LINE,I).EQ.0) RETURN + K = INDEX(LINE,' ') + LINE(K:) = '..' + IF(ITOSL(LINE(K+2:),J).EQ.0) RETURN + WPAIR = 0 + END + SUBROUTINE TRPIRP(SEQ,IDIM,I1,I2,KSTART,MINOP,IDEV,PAA, + +JSTRAN,OUTP,FRAMEC,IDIMP) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIMP),PAA(125),OUTP(IDIMP) + INTEGER B,FRAME,FRAMEC(IDIM) + EXTERNAL IPDNA + MINOPN = MINOP + 1 + DO 200 FRAME = 1,3 + B = I1 - KSTART + FRAME + LAA = IDIMP + CALL TRANSD(SEQ,IDIM, + + B,I2-KSTART+1,OUTP,LAA,PAA,JSTRAN) + LAA = (I2 - B + 1) / 3 + B = 1 + I = 0 +20 CONTINUE + I = I + 1 + IF(I.LE.LAA) THEN + IF(OUTP(I).EQ.'*') THEN + LENGTH = I - B + 1 + IF(LENGTH.GE.MINOPN) THEN + IF(JSTRAN.EQ.0) THEN + IP1 = IPDNA(B,FRAME,JSTRAN) + I1 - KSTART + IP2 = IPDNA(I,FRAME,JSTRAN) + I1 - KSTART + IP2 = MIN(IP2,IDIM) +C WRITE(IDEV,1002)IP1,IP2,SENSE(1),FRAME,IP2-IP1+1 + CALL MBPRIM(FRAMEC,IDIM,IP1,IP2,2) + ELSE IF(JSTRAN.EQ.1) THEN + IP1 = IPDNA(B,FRAME,JSTRAN) + I1 - KSTART + IF(B.NE.1) IP1 = IP1 - 3 + IP2 = IPDNA(I,FRAME,JSTRAN) + I1 - KSTART - 3 + IP2 = MIN(IP2,IDIM) +C WRITE(IDEV,1002)IP2,IP1,SENSE(2),FRAME,IP2-IP1+1 + CALL MBPRIM(FRAMEC,IDIM,IP1,IP2,3) + END IF + END IF + B = I + 1 + END IF + GO TO 20 + END IF + LENGTH = I - B + IF(LENGTH.GE.MINOPN) THEN + IF(JSTRAN.EQ.0) THEN + IP1 = IPDNA(B,FRAME,JSTRAN) + I1 - KSTART + IP2 = IPDNA(I,FRAME,JSTRAN) + I1 - KSTART + IP2 = MIN(IP2,IDIM) +C WRITE(IDEV,1002)IP1,IP2,SENSE(1),FRAME,IP2-IP1+1 + CALL MBPRIM(FRAMEC,IDIM,IP1,IP2,2) + LENGTH = LENGTH + 1 + ELSE IF(JSTRAN.EQ.1) THEN + IP1 = IPDNA(B,FRAME,JSTRAN) + I1 - KSTART + IF(B.NE.1) IP1 = IP1 - 3 + IP2 = IPDNA(I,FRAME,JSTRAN) + I1 - KSTART + IP2 = MIN(IP2,IDIM) +C WRITE(IDEV,1002)IP2,IP1,SENSE(2),FRAME,IP2-IP1+1 + CALL MBPRIM(FRAMEC,IDIM,IP1,IP2,3) + END IF + END IF +200 CONTINUE +C 1002 FORMAT(' FT ',I7,I7,' ',A,I4,I7) + END + SUBROUTINE TRAN6X(SEQ,IDIM,JDEV,I1,I2,PAA,ITRAN,JSTRAN, + +INUM,LINLEN,FC) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),TRANF4*3,TRANB4*3,SCOMP,PAA(125) + CHARACTER LN*133,TICKS*120 + INTEGER FC(IDIM) + EXTERNAL TRANF4,TRANB4,SCOMP,NOTRL + SAVE TICKS + DATA TICKS(1:60)/ + +' . : . : . : . : . : . :'/ + DATA TICKS(61:120)/ + +' . : . : . : . : . : . :'/ + K1=I1 + K2=I1+LINLEN-1 + K2 = MIN(K2,I2) + LN = ' ' +20 CONTINUE + WRITE(JDEV,1004) + WRITE(LN,1000)(TRANF4(SEQ(K),PAA,ITRAN,FC(K)),K=K1,K2-1,3) + IF(NOTRL(LN,133,' ').NE.0)WRITE(JDEV,1006)LN(1:NOTRL(LN,133,' ')) + WRITE(LN,1001)(TRANF4(SEQ(K),PAA,ITRAN,FC(K)),K=K1+1,K2,3) + IF(NOTRL(LN,133,' ').NE.0)WRITE(JDEV,1006)LN(1:NOTRL(LN,133,' ')) + IF(K1.NE.1) THEN + WRITE(LN,1002)(TRANF4(SEQ(K),PAA,ITRAN,FC(K)), + + K=K1-1,K2-2,3) + IF(NOTRL(LN,133,' ').NE.0)WRITE(JDEV,1006) + + LN(1:NOTRL(LN,133,' ')) + ELSE + WRITE(LN,1008)(TRANF4(SEQ(K),PAA,ITRAN,FC(K)),K=3,K2-2,3) + IF(NOTRL(LN,133,' ').NE.0)WRITE(JDEV,1006) + + LN(1:NOTRL(LN,133,' ')) + END IF + IF(INUM.NE.0) THEN + WRITE(JDEV,1003)(SEQ(K),K=K1,K2) + WRITE(JDEV,1005)(N,N=K1+9,K2,10) + ELSE + WRITE(LN,1003)(SEQ(K),K=K1,K2) + WRITE(LN(NOTRL(LN,133,' ')+2:),1007)K2 + WRITE(JDEV,1006)LN(1:NOTRL(LN,133,' ')) + IF(JSTRAN.NE.0) WRITE(JDEV,1009)TICKS(1:K2-K1+1) + END IF + IF(JSTRAN.NE.0)WRITE(JDEV,1003)(SCOMP(SEQ(K)),K=K1,K2) + WRITE(LN,1000)(TRANB4(SEQ(K),PAA,ITRAN,FC(K)),K=K1,K2-1,3) + IF(NOTRL(LN,133,' ').NE.0)WRITE(JDEV,1006)LN(1:NOTRL(LN,133,' ')) + WRITE(LN,1001)(TRANB4(SEQ(K),PAA,ITRAN,FC(K)),K=K1+1,K2,3) + IF(NOTRL(LN,133,' ').NE.0)WRITE(JDEV,1006)LN(1:NOTRL(LN,133,' ')) + IF(K1.NE.1) THEN + WRITE(LN,1002)(TRANB4(SEQ(K),PAA,ITRAN,FC(K)),K=K1-1,K2-2,3) + IF(NOTRL(LN,133,' ').NE.0)WRITE(JDEV,1006) + + LN(1:NOTRL(LN,133,' ')) + ELSE + WRITE(LN,1008)(TRANB4(SEQ(K),PAA,ITRAN,FC(K)),K=3,K2-2,3) + IF(NOTRL(LN,133,' ').NE.0)WRITE(JDEV,1006) + + LN(1:NOTRL(LN,133,' ')) + END IF + IF(K2.EQ.I2) RETURN + K1=K2+1 + K2=K2+LINLEN + K2 = MIN(K2,I2) + GO TO 20 +1000 FORMAT(4X,40(A)) +1001 FORMAT(5X,40(A)) +1002 FORMAT(3X,40(A)) +1003 FORMAT(4X,120A1) +1004 FORMAT( ) +1005 FORMAT(4X,12I10) + 1006 FORMAT(A) + 1007 FORMAT(I7) + 1008 FORMAT(6X,40(A)) + 1009 FORMAT(4X,A) + END + INTEGER FUNCTION IPDNA(IACID,FRAME,ISTRAN) + INTEGER FRAME +C returns a dna position for an amino acid in a translation +C frames on opposing strands: 123123123 +C 123123123 + IPDNA = FRAME + (IACID-1)*3 + END + CHARACTER*3 FUNCTION TRANF4(CODON,PAA,CODE,FRAMEC) +C AUTHOR RODGER STADEN + CHARACTER CODON(3),PAA(5,5,5),THREEL*3,TRANF,THREE*3 + INTEGER CODE,FRAMEC + EXTERNAL THREEL,TRANF + THREE(1:3)=' ' + IF(MOD(FRAMEC,2).EQ.0) THEN + IF(CODE.EQ.3)THEN + THREE(1:3)=THREEL(TRANF(CODON,PAA)) + ELSE + THREE(2:2)=TRANF(CODON,PAA) + END IF + END IF + TRANF4 = THREE + END +C TRANB3 + CHARACTER*3 FUNCTION TRANB4(CODON,PAA,CODE,FRAMEC) +C AUTHOR RODGER STADEN + CHARACTER CODON(3),PAA(5,5,5),THREEL*3,TRANB,THREE*3 + INTEGER CODE,FRAMEC + EXTERNAL THREEL,TRANB + THREE=' ' + IF(MOD(FRAMEC,3).EQ.0) THEN + IF(CODE.EQ.3)THEN + THREE(1:3)=THREEL(TRANB(CODON,PAA)) + ELSE + THREE(2:2)=TRANB(CODON,PAA) + END IF + END IF + TRANB4 = THREE(1:3) + END + SUBROUTINE HAIRPN(SEQ,IDIM,ITOT,ITOTEL,ITOTAL,IDTOT, + +MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,KSTART,IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + INTEGER ITOT(IDTOT),ITOTEL(IDTOT),ITOTAL(IDTOT) + CALL SHOWFU(KBOUT,'Search for hairpin loops') + CALL GETPAR(30,10,IOK,MINLPI,MAXLPI,MINLP, + +MINLPX,MAXLPX,MAXLP,MINBP,MAXBP,MININ,IGON, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + CALL HAIRPD(J1,J2,KBIN,KBOUT, + +MINLPI,MAXLPI,MINLP,MINLPX,MAXLPX,MAXLP,MINBP,MAXBP,MININ, + +IGON,IOK, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL HAIRPP(SEQ,IDIM,ITOT,ITOTEL,ITOTAL,IDTOT, + +MINLP,MAXLP,MININ,IGON, + +MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,KSTART,IDEV,KBOUT) + END + SUBROUTINE HAIRPP(SEQ,IDIM,ITOT,ITOTEL,ITOTAL,IDTOT, + +MINLP,MAXLP,MININ,IGON, + +MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,IS,IN,KSTART,IDEV,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER SCORES(30) + CHARACTER SEQ(IDIM) + INTEGER ITOT(IDTOT),ITOTEL(IDTOT),ITOTAL(IDTOT) + INTEGER CTONUM + EXTERNAL CTONUM,LWRAP2 + CALL BUSY(KBOUT) +C set scores as at=1,gc=1,gt=1 +C could make gt selectable + DO 1 I=1,30 +1 SCORES(I)=0 + SCORES(16)=1 + SCORES(21)=1 + SCORES(22)=1 + SCORES(8)=1 + SCORES(9)=1 + SCORES(14)=1 + IDO=IN-IS+1 + I=IS-1 + IP=0 + DO 301 K=1,IDO + I=I+1 + DO 300 J=MINLP,MAXLP + N=0 +100 CONTINUE + IMN=I-N + IPNPJ=I+N+J + IT=CTONUM(SEQ(LWRAP2(IN,IMN)))+ + + 5*CTONUM(SEQ(LWRAP2(IN,IPNPJ))) + IT=SCORES(IT) + IF(IT.NE.0)THEN + N=N+IT + GO TO 100 + END IF +C IS THIS LOOP WORTH KEEPING? + IF(N.GE.MININ)THEN + IP=IP+1 + IF(IP.GT.IDTOT)THEN + WRITE(KBOUT,1234)IP,I +1234 FORMAT(' Maximum of',I5, + + ' loops found up to',I6,' No more searching') + GO TO 302 + END IF + ITOT(IP)=N + ITOTEL(IP)=LWRAP2(IN,I) + ITOTAL(IP)=J-1 + END IF +300 CONTINUE +301 CONTINUE +302 CONTINUE + IF(IP.GT.0)THEN + NOUT=0 + DO 700 J=1,IP + JP1=J+1 + ID1 = ITOTEL(J) + ITOTAL(J)/2 + DO 690 I=JP1,IP + ID2 = ITOTEL(I) + ITOTAL(I)/2 + IF(ID1.NE.ID2)GO TO 690 + LP2=ITOTAL(I)+2*ITOT(I) + IF(ITOTAL(J).GT.LP2)GO TO 690 + ITOT(J)=0 + ITOTAL(J)=0 + ITOTEL(J)=0 + NOUT=NOUT+1 + GO TO 700 +690 CONTINUE +700 CONTINUE + CALL BUB3AS(ITOTEL,ITOT,ITOTAL,IP) + IF(IGON.EQ.0)THEN + XMAX=IN + XMIN=IS +C PLOT ON SCALE 0 TO 4*MININ IN Y + YMIN=0. + YMAX=MININ*4 + YF=0. + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + DO 800 I=NOUT+1,IP + XF=ITOTEL(I)+ITOTAL(I)/2 + XT=XF + YT=ITOT(I) + CALL LINE(XF,XT,YF,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +800 CONTINUE + CALL VT100M + ELSE IF(IGON.EQ.1) THEN + DO 900 I=NOUT+1,IP + CALL DRAWPN( + + SEQ,IDIM,ITOT(I),ITOTAL(I),ITOTEL(I), + + IDEV,IN,KSTART) +900 CONTINUE + END IF + END IF + WRITE(KBOUT,1005)IP-NOUT +1005 FORMAT(' Total loops found=',I6) + END + SUBROUTINE HAIRPD(IS,IN,KBIN,KBOUT, + +MINLPI,MAXLPI,MINLP,MINLPX,MAXLPX,MAXLP,MINBP,MAXBP,MININ, + +IGON,IOK, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + WRITE(KBOUT,1004) +1004 FORMAT(' Define the range of loop sizes') + CALL GETINT(MINLPI,MAXLPI,MINLP,'Minimum loop size', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINLP = IVAL + MINLPX = MAX(MINLPX,MINLP) + MAXLP = MINLPX + CALL GETINT(MINLPX,MAXLPX,MAXLP,'Maximum loop size', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXLP = IVAL + MINLP = MINLP + 1 + MAXLP = MAXLP + 1 + CALL GETINT(MINBP,MAXBP,MININ,'Minimum number of basepairs', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MININ = IVAL + CALL YESONO(IGON,'Plot results','List results', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) +C IF(IGON.LT.0)RETURN + END +C +C subroutine to draw hairpin loops on device idev +C hairpins are defined by: +C 1) nstem the number of basepairs in the stem +C 2) nloop the number of bases in the loop +C 3) ip the position of the rightmost base in the left side of the stem +C +C draw the stems very simply, just standing up with a few bases +C before and after +C maximum size is 20 up and 14 across + SUBROUTINE DRAWPN(SEQ,IDIM,NSTEM,NLOOP,IP,IDEV,IN,KSTART) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),ARRAY(14,20),SCORE(5,5),SPACE + INTEGER CTONUM + EXTERNAL CTONUM + SAVE SPACE + DATA SPACE/' '/ +C CHECK FOR WRAP AROUND + IF((IP-NSTEM).LT.0)RETURN + IF((IP+NLOOP+NSTEM+1).GT.IN)RETURN + DO 1 I=1,5 + DO 1 J=1,5 + SCORE(I,J)=' ' +1 CONTINUE + SCORE(1,3)='-' + SCORE(3,1)='-' + SCORE(2,4)='-' + SCORE(4,2)='-' + SCORE(1,4)='.' + SCORE(4,1)='.' + DO 2 I=1,14 + DO 2 J=1,20 + ARRAY(I,J)=SPACE +2 CONTINUE +C point to left stem start + IPL=IP-NSTEM +C point to right stem start + IPR=IP+NSTEM+NLOOP+1 +C point to temporary array + IAL=6 + IAR=8 + KA=1 +C fill in stem and loop + DO 10 I=1,MIN(NSTEM+NLOOP/2,19) + IPL=IPL+1 + IPR=IPR-1 + KA=KA+1 + ARRAY(IAL,KA)=SEQ(IPL) + ARRAY(IAR,KA)=SEQ(IPR) +C basepaired? + ARRAY(IAL+1,KA)= + + SCORE(CTONUM(SEQ(IPL)),CTONUM(SEQ(IPR))) +10 CONTINUE +C odd number of bases in loop? + IF(MOD(NLOOP,2).NE.0)THEN +C put in extra base + IF((KA+1).LE.20)ARRAY((IAL+1),KA+1)=SEQ(IPL+1) + END IF +C20 CONTINUE +C now do 6 bases before the stem and 7 after + IPL=IP-NSTEM-6 + DO 30 I=1,6 + IPL=IPL+1 + IF(IPL.GT.0)ARRAY(I,1)=SEQ(IPL) +30 CONTINUE + IPR=IP+NSTEM+NLOOP + DO 40 I=8,14 + IPR=IPR+1 + IF(IPR.LT.IN)ARRAY(I,1)=SEQ(IPR) +40 CONTINUE + DO 50 I=1,20 + K=21-I + WRITE(IDEV,1000)(ARRAY(L,K),L=1,14) +50 CONTINUE +1000 FORMAT(' ',5X,14A1) +1001 FORMAT(' ',5X,I6) + WRITE(IDEV,1001)IP-NSTEM+KSTART-1 + RETURN + END + SUBROUTINE WORDFP(SEQ,IDIM1P,IDIM1,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN +C ROUTINE TO COMPARE OBSERVED WITH EXPECTED 'WORD' FREQUENCIES +C WORDS MAY BE UP TO 10 CHARACTERS IN LENGTH ALTHOUGH, AS THE +C EXPECTED FREQUENCIES ARE CALCULATED SIMPLY BY MULTIPLYING +C THE BASE FREQUENCIES TOGETHER, THE LONGER THE WORD THE MORE +C INAPPROPRIATE THE MODEL. USERS ARE SIMPLY PROMPTED FOR THE WORD +C TO SEARCH FOR - E.G CG - AND A WINDOW LENGTH TO COUNT OVER. +C THE PROGRAM PLOTS (OBSERVED - EXPECTED) +C IF IT IS TOO SLOW, FASTER ALGORITHMS ARE POSSIBLE. + CHARACTER SEQ(IDIM1P),WORD(10),HELPF*(*) + INTEGER SPAN + CALL SHOWFU(KBOUT,'Plot composition differences (obs-exp))') + WORD(1) = 'C' + WORD(2) = 'G' + LENGTH = 2 + CALL GETPAR(64,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + CALL WORDFD(WORD,LENGTH,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +YMIN,YMAX,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL WRDFP(SEQ,IDIM1P,IDIM1,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART, + +YMIN,YMAX,SPAN,IWRIT,WORD,LENGTH) + END + SUBROUTINE WORDFD(WORD,LENGTH,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +YMIN,YMAX,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) +C AUTHOR: RODGER STADEN + CHARACTER WORD(10),WORDA(10),HELPF*(*) + INTEGER SPAN + CALL SQPF3(WORD,WORDA,10,LENGTH,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + YMAX1 = REAL(SPAN)/(4**LENGTH) + YMIN1 = -1.0*YMAX1 + YMIN2 = 4.*YMIN1 + YMAX2 = 4.*YMAX1 + CALL GETRL(YMIN1,YMAX2,YMAX1, + +'Maximum plot value',VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + YMAX = VALUE + CALL GETRL(YMIN2,YMAX,YMIN1, + +'Minimum plot value',VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + YMIN = VALUE + IOK = 0 + END + SUBROUTINE WRDFP(SEQ,IDIM1P,IDIM1,MXSPAN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART, + +YMIN,YMAX,SPAN,IWRIT,WORD,LENGTH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P),WORD(10) + INTEGER SPAN,CTONUM,IP(10),OBS(5) + EXTERNAL CTONUM + CALL CLEARV + CALL FILLI(OBS,5,0) + DO 15 I = 1,LENGTH + IP(I) = CTONUM(WORD(I)) +15 CONTINUE + I1INM1=1+(MXSPAN/2)-KSTART+1 + INC = 1 + J1P=J1+I1INM1 + J2P=J2+I1INM1 + XMIN=J1 + XMAX=J2 + LF=SPAN/2 + LB=1+SPAN/2 + DO 66 I=J1P,J1P+SPAN-1 + IS=CTONUM(SEQ(I)) + OBS(IS)=OBS(IS)+1 +66 CONTINUE + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XF = J1 + XT = J2 + YF = YMIN + (YMAX-YMIN)/2. + YT = YF + CALL LINE(XF,XT,YF,YT,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + MARG = NINT(0.05*MARGT) + MARGB1 = MARGB+MARG + MARGT1 = MARGT - 2*MARG + XF=J1 + YF=0. + DO 100 I=J1P+LB,J2P-(LENGTH-1) + ISF=CTONUM(SEQ(I+LF)) + ISB=CTONUM(SEQ(I-LB)) + OBS(ISF)=OBS(ISF)+1 + OBS(ISB)=OBS(ISB)-1 + IF(MOD(I,IWRIT).EQ.0)THEN + EXPEC = 1.0 + DO 80 J=1,LENGTH + EXPEC = EXPEC * REAL(OBS(IP(J)))/SPAN +80 CONTINUE + EXPEC = EXPEC * SPAN + NFOUND = 0 + IAT=I-LB + IEND = I+LF +90 CONTINUE + IAT=IAT+INC + IDIM = IEND - IAT + 1 + IF(IDIM.GT.0)THEN + CALL FIND6(SEQ(IAT),IDIM,WORD,LENGTH,INC,JMATCH) + IF(JMATCH.NE.0)THEN + IAT=IAT+JMATCH-1 + NFOUND = NFOUND + 1 + GO TO 90 + END IF + END IF + SUM = NFOUND - EXPEC + XT=I-I1INM1 + CALL LINE(XF,XT,YF,SUM,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB1,MARGT1,ISXMAX,ISYMAX) + XF=XT + YF=SUM + END IF +100 CONTINUE + CALL VT100M + END + SUBROUTINE BPHASE(SEQ,IDIM1P,IDIM1,MXSPAN, + +ISXMAX,ISYMAX,MARGL1,MARGR1,MARGB1,MARGT1,J1,J2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,DIALOG + CALL SHOWFU(KBOUT,'Uneven positional base frequencies method') + CALL GETPAR(44,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + MAXSP = MIN(MAXSP,IDIM1/3) + SPAN = MIN(MAXSP,SPAN) + IF(DIALOG.EQ.1) THEN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL BPHASP(SEQ,IDIM1P,IDIM1,MXSPAN, + +ISXMAX,ISYMAX,MARGL1,MARGR1,MARGB1,MARGT1,J1,J2,SPAN,IWRIT) + END + SUBROUTINE BPHASP(SEQ,IDIM1P,IDIM1,MXSPAN, + +ISXMAX,ISYMAX,MARGL1,MARGR1,MARGB1,MARGT1,J1,J2,LENW,IWRIT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + REAL POS(5,3),COMP(5),POS1(15) + EQUIVALENCE (POS,POS1) + I1IN=2+MXSPAN/2 + I1INM1=I1IN-1 + YMIN=0.4 + YMAX=1.34 + IDIMJ=J2-J1+1 + J1P=J1+I1INM1 + J2P=J2+I1INM1 + LB=1+LENW/2 + LB=3*LB + LF=LENW/2 + LF=3*LF + LENW3=3*LENW + IDONE=0 + XMIN=J1 + XMAX=J2 + CALL CLEARV + CALL VECTOM + XP=J1 + YP=0. + X=J2 + CALL FRAME(MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) +C SET UP MARGINS + MARGB=MARGB1+NINT(0.006*ISYMAX) + MARGT=MARGT1-NINT(0.012*ISYMAX) + CALL LINE(XP,X,.78,.78,XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB,MARGT,ISXMAX,ISYMAX) + CALL TEXT(XMIN,0.78,'76%',3,0,XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB,MARGT,ISXMAX,ISYMAX) + CALL BPHAS1(SEQ(J1P-LB),IDIM1,POS,COMP,LENW3,POS1) + DO 300 IP=J1P,J2P,3 + IDONE=IDONE+1 + TESTC=ROTPHS(SEQ,IDIM1P,IP,LB,LF,POS,COMP) + IF(MOD(IDONE,IWRIT).EQ.0)THEN + X=IP-I1INM1 + CALL LINE(XP,X,YP,TESTC,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB,MARGT,ISXMAX,ISYMAX) + YP=TESTC + XP=X + END IF +300 CONTINUE + CALL VT100M + END + REAL FUNCTION ROTPHS(SEQ,IDIM,IAT,LB,LF,POS,BCOMP) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + REAL POS(5,3),BCOMP(5),DIFF(5,3),DIFF1(15) + INTEGER CTONUM + EXTERNAL CTONUM + EQUIVALENCE (DIFF,DIFF1) +C zero variables + CALL FILLR(DIFF1,15,0.) +C LOOK BACK TO SUBTRACT A CODON + IPB=IAT-LB +C sum for each base for each codon position + K=0 + DO 10 J=IPB,IPB+2 + K=K+1 + JJ=CTONUM(SEQ(J)) + BCOMP(JJ)=BCOMP(JJ)-1. + POS(JJ,K)=POS(JJ,K)-1. +10 CONTINUE +C look forwards and add a value + IPF=IAT+LF + K=0 + DO 20 J=IPF,IPF+2 + K=K+1 + JJ=CTONUM(SEQ(J)) + BCOMP(JJ)=BCOMP(JJ)+1. + POS(JJ,K)=POS(JJ,K)+1. +20 CONTINUE + DO 30 I=1,4 + TEMP=BCOMP(I)/3. + DO 30 J=1,3 + DIFF(I,J)=ABS(POS(I,J)-TEMP) +30 CONTINUE + R=0. + DO 60 I=1,4 + DO 60 J=1,3 + IF(BCOMP(I).NE.0.)R=R+DIFF(I,J)/BCOMP(I) +60 CONTINUE + ROTPHS=R + END + SUBROUTINE BPHAS1(SEQ,IDIM,POS,COMP,LWIND3,POS1) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + REAL POS(5,3),COMP(5),POS1(15) + INTEGER CTONUM + EXTERNAL CTONUM + CALL FILLR(POS1,15,0.) + CALL FILLR(COMP,5,0.) + DO 10 I=1,LWIND3-1,3 + K=0 + I1=I + I2=I1+2 + DO 9 J=I1,I2 + K=K+1 + JJ=CTONUM(SEQ(J)) + COMP(JJ)=COMP(JJ)+1. + POS(JJ,K)=POS(JJ,K)+1. +9 CONTINUE +10 CONTINUE + END + SUBROUTINE FICKET(SEQ,IDIM1P,IDIM1,MXSPAN, + +ISXMAX,ISYMAX,MARGL1,MARGR1,MARGB1,MARGT1,J1,J2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,DIALOG + CALL SHOWFU(KBOUT,'Ficketts method to find protein genes') + CALL GETPAR(48,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + IF(DIALOG.EQ.1) THEN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL FICKTP(SEQ,IDIM1P,IDIM1,MXSPAN, + +ISXMAX,ISYMAX,MARGL1,MARGR1,MARGB1,MARGT1,J1,J2,SPAN,IWRIT) + END + SUBROUTINE FICKTP(SEQ,IDIM1P,IDIM1,MXSPAN, + +ISXMAX,ISYMAX,MARGL1,MARGR1,MARGB1,MARGT1,J1,J2,LENW,IWRIT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P) + REAL POS(5,3),COMP(5),POS1(15) + EQUIVALENCE (POS,POS1) + EXTERNAL ROTFCK + I1IN=2+MXSPAN/2 + I1INM1=I1IN-1 + IDIMJ=J2-J1+1. + J1P=J1+I1INM1 + J2P=J2+I1INM1 + YMIN=0. + YMAX=1. + XMIN=J1 + XMAX=J2 + LB=1+LENW/2 + LB=3*LB + LF=LENW/2 + LF=3*LF + LENW3=3*LENW +C calc fraction of window length for composition + FRAC=1./LENW3 + IDONE=0 + CALL CLEARV + CALL VECTOM +C set initial values + XP=J1 + YP=0. + X=J2 + CALL FRAME(MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL LINE(XP,X,.4,.4,XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL LINE(XP,X,.77,.77,XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL FCKSTR(SEQ(J1P-LB),IDIM1,POS,COMP,LENW3,POS1) + DO 300 IP=J1P,J2P,3 + IDONE=IDONE+1 + TESTC=ROTFCK(SEQ,IDIM1P,IP,LB,LF,POS,COMP,FRAC) + IF(MOD(IDONE,IWRIT).EQ.0)THEN + X=IP-I1INM1 + CALL LINE(XP,X,YP,TESTC,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + YP=TESTC + XP=X + END IF +300 CONTINUE + CALL VT100M + END + REAL FUNCTION ROTFCK(SEQ,IDIM,IAT,LB,LF,POS,BCOMP,FRAC) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + REAL POS(5,3),BASMAX(4),BASMIN(4),BPOS(4),BCOMP(5),BCOMPT(5) + REAL WCOMP(4),WPOS(4),PCODP(10,4),PCODC(10,4),PCODCR(10) + REAL PCODPR(10) + INTEGER CTONUM + EXTERNAL CTONUM + SAVE WCOMP,WPOS,PCODCR,PCODPR,PCODP,PCODC + DATA WCOMP/.14,.12,.11,.15/ + DATA WPOS/.33,.18,.26,.31/ + DATA PCODCR/.17,.19,.21,.23,.25,.27,.29,.31,.33,.99/ + DATA PCODPR/1.1,1.2,1.3,1.4,1.5,1.6,1.7,1.8,1.9,99./ + DATA PCODC/.58,.51,.69,.56,.75,.55,.40,.39,.24,.28, + 1.31,.39,.44,.43,.59,.59,.64,.51,.64,.82,.21,.81,.65,.67, + 2.49,.62,.55,.44,.49,.28,.29,.33,.41,.41,.73,.64,.64,.47, + 3.54,.40/ + DATA PCODP/.09,.09,.20,.54,.44,.69,.68,.91,.97,.97, + 1.23,.30,.33,.51,.48,.66,.81,.70,.70,.80, + 2.22,.20,.34,.45,.68,.58,.93,.84,.68,.94, + 3.08,.08,.16,.27,.48,.53,.64,.74,.88,.90/ +C DATA TESTCP/.00,.04,.07,.29,.40,.77,.92,.98,1.0,1.0/ +C DATA TESTCR/.43,.53,.64,.74,.84,.95,1.05,1.16,1.26,99./ + DO 5 I=1,4 + BASMAX(I)=0. + BASMIN(I)=IDIM + BPOS(I)=0. +5 CONTINUE +C look back to subtract a codon + IPB=IAT-LB +C sum for each base for each codon position + K=0 + DO 10 J=IPB,IPB+2 + K=K+1 + JS=CTONUM(SEQ(J)) + BCOMP(JS)=BCOMP(JS)-FRAC + POS(JS,K)=POS(JS,K)-1. +10 CONTINUE +C look forwards and add a value + IPF=IAT+LF + K=0 + DO 20 J=IPF,IPF+2 + K=K+1 + JS=CTONUM(SEQ(J)) + BCOMP(JS)=BCOMP(JS)+FRAC + POS(JS,K)=POS(JS,K)+1. +20 CONTINUE +C get max and min values for each base in each codon position + DO 30 J=1,3 + DO 30 I=1,4 + IF(POS(I,J).GT.BASMAX(I))BASMAX(I)=POS(I,J) + IF(POS(I,J).LT.BASMIN(I))BASMIN(I)=POS(I,J) +30 CONTINUE + DO 40 I=1,4 + BPOS(I)=BASMAX(I)/(BASMIN(I)+1.) +40 CONTINUE +C look up prob of coding + DO 46 I=1,4 + DO 45 J=1,10 + IF(BCOMP(I).GT.PCODCR(J))GO TO 45 +C lt current so set prob + BCOMPT(I)=PCODC(J,I) + GO TO 46 +45 CONTINUE +46 CONTINUE + DO 48 I=1,4 + DO 47 J=1,10 + IF(BPOS(I).GT.PCODPR(J))GO TO 47 +C lt current so set prob + BPOS(I)=PCODP(J,I) + GO TO 48 +47 CONTINUE +48 CONTINUE +C apply weights + DO 50 I=1,4 + BCOMPT(I)=BCOMPT(I)*WCOMP(I) + BPOS(I)=BPOS(I)*WPOS(I) +50 CONTINUE +C calc testcode + TESTC=0. + DO 60 I=1,4 + TESTC=TESTC+BPOS(I)+BCOMPT(I) +60 CONTINUE + ROTFCK=TESTC + END + SUBROUTINE FCKSTR(SEQ,IDIM,POS,COMP,LWIND3,POS1) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + REAL POS(5,3),COMP(5),POS1(15) + INTEGER CTONUM + EXTERNAL CTONUM +C calc fraction to add ie window length recip + FRAC=1./LWIND3 +C zero variables + CALL FILLR(COMP,5,0.) + CALL FILLR(POS1,15,0.) +C sum for each base for each codon position + DO 10 I=1,LWIND3-1,3 + K=0 + I1=I + I2=I1+2 + DO 9 J=I1,I2 + K=K+1 + JS=CTONUM(SEQ(J)) + COMP(JS)=COMP(JS)+FRAC + POS(JS,K)=POS(JS,K)+1. +9 CONTINUE +10 CONTINUE + END + SUBROUTINE SHEPED(SEQ,IDIM1P,IDIM1,MXSPAN, + +ISXMAX,ISYMAX,J1,J2,MARGL,MARGR,MARGB,MARGT,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PAA,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1P),PAA(5,5,5) + INTEGER SPAN,DIALOG + CALL SHOWFU(KBOUT,'Shepherds method to find protein genes') + CALL GETPAR(47,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + IF(DIALOG.EQ.1) THEN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL SHEPP(SEQ,IDIM1P,IDIM1,MXSPAN, + +ISXMAX,ISYMAX,J1,J2,MARGL,MARGR,MARGB,MARGT,PAA,SPAN,IWRIT) + END + SUBROUTINE SHEPP(SEQ,IDIM1P,IDIM1,MXSPAN, + +ISXMAX,ISYMAX,J1,J2,MARGL,MARGR,MARGB,MARGT,PAA,LENW,IWRIT) +C AUTHOR: RODGER STADEN + REAL SUMW(3),PROB(3) + CHARACTER SEQ(IDIM1P),PAA(5,5,5) + CHARACTER SPACE,RLINE(3),STAR + SAVE SPACE,STAR + DATA SPACE,STAR/' ','*'/ + IMH=NINT(0.005*ISYMAX) + I1IN=2+MXSPAN/2 + I1INM1=I1IN-1 + IDIMJ=J2-J1+1. + J1P=J1+I1INM1 + J2P=J2+I1INM1 + YMIN=0.2 + YMAX=0.4 + YMID=YMIN+(YMAX-YMIN)/2. + XMIN=J1 + XMAX=J2 +C SORT OUT MARGIN IE FIND MAX AND DIVIDE BY THREE + IMARG=NINT(MARGT/3.) + MARGL1=MARGL + MARGR1=MARGR + MARGB1=MARGB + MARGB2=MARGB+IMARG + MARGB3=MARGB+2*IMARG + MARGT3=IMARG + MARGT2=IMARG + MARGT1=IMARG + LB=1+LENW/2 + LB=3*LB + LF=LENW/2 + LF=3*LF + LENW3=3*LENW + CALL FILLR(SUMW,3,0.) + IDONE=0 + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL FRAME(MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + CALL FRAME(MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) + CALL SHEPST(SEQ(J1P-LB),IDIM1,LENW3,SUMW(1)) + CALL SHEPST(SEQ(J1P+1-LB),IDIM1,LENW3,SUMW(2)) + CALL SHEPST(SEQ(J1P+2-LB),IDIM1,LENW3,SUMW(3)) +C set initial values + XP=J1 + YP1=0.5 + YP2=0.5 + YP3=0.5 + DO 300 IP=J1P,J2P,3 + IDONE=IDONE+1 + IP0=IP + IP1=IP+1 + IP2=IP+2 + CALL RTSHEP(SEQ,IDIM1P,IP0,LB,LF,SUMW(1)) + CALL RTSHEP(SEQ,IDIM1P,IP1,LB,LF,SUMW(2)) + CALL RTSHEP(SEQ,IDIM1P,IP2,LB,LF,SUMW(3)) + IF(MOD(IDONE,IWRIT).EQ.0)THEN + T=SUMW(1)+SUMW(2)+SUMW(3) + PROB(1)=SUMW(1)/T + PROB(2)=SUMW(2)/T + PROB(3)=SUMW(3)/T +C get largest + DO 302 II=1,3 + RLINE(II)=SPACE +302 CONTINUE + TP=MAX(SUMW(1),SUMW(2),SUMW(3)) + DO 303 II=1,3 + IF(SUMW(II).EQ.TP)RLINE(II)=STAR +303 CONTINUE +C set actual ip + IPA=IP-I1INM1 + X=IPA + Y1=PROB(1) + Y2=PROB(2) + Y3=PROB(3) + CALL LINE(XP,X,YP1,Y1,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1+IMH,MARGT1-2*IMH,ISXMAX,ISYMAX) + CALL LINE(XP,X,YP2,Y2,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB2+IMH,MARGT2-2*IMH,ISXMAX,ISYMAX) + CALL LINE(XP,X,YP3,Y3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB3+IMH,MARGT3-2*IMH,ISXMAX,ISYMAX) + IF(RLINE(1).EQ.STAR) + + CALL POINT(X,YMID,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + IF(RLINE(2).EQ.STAR) + + CALL POINT(X,YMID,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + IF(RLINE(3).EQ.STAR) + + CALL POINT(X,YMID,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) + YP1=Y1 + YP2=Y2 + YP3=Y3 + XP=X + END IF +300 CONTINUE + CALL STARTS(SEQ,IDIM1P,J1P,J2P, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,PAA) + CALL STOPS(SEQ,IDIM1P,J1P,J2P, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,PAA) + CALL VT100M + END + SUBROUTINE SHEPST(SEQ,IDIM,LENW,SUM) +C AUTHOR: RODGER STADEN + REAL SUM + CHARACTER SEQ(IDIM) + INTEGER SCORER(5),SCOREY(5),CTONUM + EXTERNAL CTONUM + SAVE SCORER,SCOREY + DATA SCORER,SCOREY/0,0,1,1,0,1,1,0,0,0/ + SUM=0. + DO 100 I=1,LENW,3 + SUM=SUM+SCORER(CTONUM(SEQ(I)))+SCOREY(CTONUM(SEQ(I+2))) +100 CONTINUE + END + SUBROUTINE RTSHEP(SEQ,IDIM1,I,LB,LF,H) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1) + INTEGER SCORER(4),SCOREY(4),CTONUM + EXTERNAL CTONUM + SAVE SCORER,SCOREY + DATA SCORER,SCOREY/0,0,1,1,1,1,0,0/ +C set up dummy values + XS=0. + XA=0. +C look back + IPB=I-LB-1 + DO 10 J=1,3 + IF(CTONUM(SEQ(IPB+J)).EQ.5)GO TO 30 +10 CONTINUE +C set value to subtract + IPB=IPB+1 + IT1=CTONUM(SEQ(IPB)) + IT2=CTONUM(SEQ(IPB+2)) + XS=SCORER(IT1)+SCOREY(IT2) +C now look forward +30 CONTINUE + IPB=I+LF-1 +C look for bad char + DO 45 J=1,3 + IF(CTONUM(SEQ(IPB+J)).EQ.5)GO TO 50 +45 CONTINUE +C ok so use + IPB=IPB+1 + IT1=CTONUM(SEQ(IPB)) + IT2=CTONUM(SEQ(IPB+2)) + XA=SCORER(IT1)+SCOREY(IT2) +50 CONTINUE + H=H-XS+XA + END + SUBROUTINE IMPBC(SEQ,IDIM1P,IDIM1,MXSPAN,FTABLE,IDFTAB, + +ISXMAX,ISYMAX,J1,J2,MARGL,MARGR,MARGB,MARGT,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PAA,DIALOG) + CHARACTER HELPF*(*),PAA(5,5,5) +C AUTHOR: RODGER STADEN + INTEGER SPAN,DIALOG + REAL FTABLE(IDFTAB) + CHARACTER SEQ(IDIM1P) + CALL SHOWFU(KBOUT,'McLachlan,Staden,Boswell codon improbability') +C WRITE(KBOUT,*)' Search for protein genes using McLachlan, Staden' +C WRITE(KBOUT,*)' and Boswell improbability method' +C WRITE(KBOUT,*)' expecting codon composition to depend on' +C WRITE(KBOUT,*)' base composition' + CALL GETPAR(45,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(DIALOG.EQ.1) THEN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL IMPBCP(SEQ,IDIM1P,IDIM1,MXSPAN,FTABLE,IDFTAB, + +ISXMAX,ISYMAX,J1,J2,MARGL,MARGR,MARGB,MARGT,PAA,SPAN,IWRIT) + END + SUBROUTINE IMPBCP(SEQ,IDIM1P,IDIM1,MXSPAN,FTABLE,IDFTAB, + +ISXMAX,ISYMAX,J1,J2,MARGL,MARGR,MARGB,MARGT,PAA,LENW,IWRIT) + CHARACTER PAA(5,5,5) +C AUTHOR: RODGER STADEN + REAL RNBASE(4) + REAL W(3),RNFAC(3),COMP(3),FTABLE(IDFTAB),FACN + INTEGER NBASE1(4),NBASE2(4),NBASE3(4) + INTEGER NCOD1(4,4,4),NCOD2(4,4,4),NCOD3(4,4,4) + CHARACTER SEQ(IDIM1P),RLINE(3),STAR + SAVE STAR + DATA STAR/'*'/ +C SORT OUT MARGIN IE FIND MAX AND DIVIDE BY THREE + IMARG=NINT(MARGT/3.) + MARGL1=MARGL + MARGR1=MARGR + MARGB1=MARGB + MARGB2=MARGB+IMARG + MARGB3=MARGB+2*IMARG + MARGT3=IMARG + MARGT2=IMARG + MARGT1=IMARG + IMH=NINT(0.005*ISYMAX) + SD=4.1 + EXPM=0. + YMIN=EXPM + YMAX=EXPM+12.*SD + IBH=NINT(0.005*ISXMAX) +C ONLY CALC FACTORS UPTO 99! + CALL FACTAB(FTABLE,99) + I1IN=2+MXSPAN/2 + I1INM1=I1IN-1 + IDIMJ=J2-J1+1. + J1P=J1+I1INM1 + J2P=J2+I1INM1 + LB=1+LENW/2 + LB=3*LB + LF=LENW/2 + LF=3*LF + LENW3=3*LENW + RLENW3=LENW3 + FACN=FACTOR(LENW,FTABLE,IDFTAB) + XMIN=J1 + XMAX=J2 + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL FRAME(MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + CALL FRAME(MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) + CALL SCALES(XMAX,XMIN,YMAX,YMIN,MARGL1,MARGR1, + +MARGB1,MARGT1,ISXMAX,ISYMAX,SD,IBH,YMIN,2) + CALL SCALES(XMAX,XMIN,YMAX,YMIN,MARGL1,MARGR1, + +MARGB2,MARGT2,ISXMAX,ISYMAX,SD,IBH,YMIN,2) + CALL SCALES(XMAX,XMIN,YMAX,YMIN,MARGL1,MARGR1, + +MARGB3,MARGT3,ISXMAX,ISYMAX,SD,IBH,YMIN,2) + CALL ROT1(SEQ(J1P-LB),IDIM1,NCOD1,RNFAC(1),FTABLE, + +IDFTAB,NBASE1,LENW3) + CALL ROT1(SEQ(J1P+1-LB),IDIM1,NCOD2,RNFAC(2),FTABLE, + +IDFTAB,NBASE2,LENW3) + CALL ROT1(SEQ(J1P+2-LB),IDIM1,NCOD3,RNFAC(3),FTABLE, + +IDFTAB,NBASE3,LENW3) +C set initial values + XP=J1 + YP1=0. + YP2=0. + YP3=0. + IDONE=0 + DO 300 IP=J1P,J2P,3 + IDONE=IDONE+1 + IP0=IP + IP1=IP+1 + IP2=IP+2 + CALL ROTFAC(SEQ,IDIM1P,IP0,LB,LF,NCOD1,RNFAC(1), + + FTABLE,IDFTAB,NBASE1,COMP(1),LENW3) + CALL ROTFAC(SEQ,IDIM1P,IP1,LB,LF,NCOD2,RNFAC(2), + + FTABLE,IDFTAB,NBASE2,COMP(2),LENW3) + CALL ROTFAC(SEQ,IDIM1P,IP2,LB,LF,NCOD3,RNFAC(3), + + FTABLE,IDFTAB,NBASE3,COMP(3),LENW3) + DO 200 K=1,3 + W(K)=RNFAC(K)-COMP(K)-FACN +200 CONTINUE + IF(MOD(IDONE,IWRIT).EQ.0)THEN + DO 302 II=1,3 + RLINE(II)=' ' +302 CONTINUE + T=MAX(W(1),W(2),W(3)) + DO 303 II=1,3 + IF(W(II).EQ.T)RLINE(II)=STAR +303 CONTINUE + IPA=IP-I1INM1 + DO 787 JJJ=1,4 + RNBASE(JJJ)=FLOAT(NBASE1(JJJ))/RLENW3 +787 CONTINUE + CALL POISON(LENW,RNBASE,RNBASE,EW,WD,1) + YMAX=EXPM+12.*WD + WD3=WD*6. + X=IPA + Y1=W(1)-EW + Y2=W(2)-EW + Y3=W(3)-EW + CALL LINE(XP,X,YP1,Y1,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1+IMH,MARGT1-2*IMH,ISXMAX,ISYMAX) + CALL LINE(XP,X,YP2,Y2,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB2+IMH,MARGT2-2*IMH,ISXMAX,ISYMAX) + CALL LINE(XP,X,YP3,Y3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB3+IMH,MARGT3-2*IMH,ISXMAX,ISYMAX) + IF(RLINE(1).EQ.STAR)CALL POINT(X,WD3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + IF(RLINE(2).EQ.STAR)CALL POINT(X,WD3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + IF(RLINE(3).EQ.STAR)CALL POINT(X,WD3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) + YP1=Y1 + YP2=Y2 + YP3=Y3 + XP=X + END IF +300 CONTINUE + CALL STARTS(SEQ,IDIM1P,J1P,J2P, + +MARGL1,MARGR1,MARGB,MARGT,ISXMAX,ISYMAX,PAA) + CALL STOPS(SEQ,IDIM1P,J1P,J2P, + +MARGL1,MARGR1,MARGB,MARGT,ISXMAX,ISYMAX,PAA) + CALL VT100M + END + SUBROUTINE IMPAC(SEQ,IDIM1P,IDIM1,MXSPAN,FTABLE,IDFTAB, + +ISXMAX,ISYMAX,J1,J2,MARGL,MARGR,MARGB,MARGT,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PAA,DIALOG) + CHARACTER HELPF*(*),PAA(5,5,5) +C AUTHOR: RODGER STADEN + REAL FTABLE(IDFTAB) + CHARACTER SEQ(IDIM1P) + INTEGER SPAN,DIALOG + CALL SHOWFU(KBOUT,'McLachlan,Staden,Boswell codon improbability') +C WRITE(KBOUT,*)' Search for protein genes using McLachlan, Staden' +C WRITE(KBOUT,*)' and Boswell improbability method' +C WRITE(KBOUT,*)' expecting codon composition to depend on' +C WRITE(KBOUT,*)' amino acid composition' + CALL GETPAR(46,6,IOK,MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT, + +IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(DIALOG.EQ.1) THEN + CALL GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL IMPACP(SEQ,IDIM1P,IDIM1,MXSPAN,FTABLE,IDFTAB, + +ISXMAX,ISYMAX,J1,J2,MARGL,MARGR,MARGB,MARGT,PAA,SPAN,IWRIT) + END + SUBROUTINE IMPACP(SEQ,IDIM1P,IDIM1,MXSPAN,FTABLE,IDFTAB, + +ISXMAX,ISYMAX,J1,J2,MARGL,MARGR,MARGB,MARGT,PAA,LENW,IWRIT) + CHARACTER PAA(5,5,5) +C AUTHOR: RODGER STADEN + REAL W(3),RNFAC(3),COMP(3),FTABLE(IDFTAB),FACN + INTEGER NBASE1(4),NBASE2(4),NBASE3(4) + INTEGER NCOD1(4,4,4),NCOD2(4,4,4),NCOD3(4,4,4) + INTEGER NCODON(21),IACID(4,4,4) + REAL FABC1(4,4,4),FA1(21) + REAL FABC2(4,4,4),FA2(21) + REAL FABC3(4,4,4),FA3(21) + CHARACTER SEQ(IDIM1P),RLINE(3),STAR + SAVE IACID,NCODON,STAR + DATA STAR/'*'/ +C array with number of codons per acid + DATA IACID/ + +1,2,3,5,6,7,8,9, + +10,12,14,16,18,20,6,21, + +1,2,3,5,6,7,8,9, + +10,12,14,16,18,20,6,21, + +2,2,3,5,6,7,8,9, + +11,13,15,17,11,20,20,21, + +2,2,4,5,6,7,8,9, + +11,13,15,17,19,20,20,21/ + DATA NCODON/ + +2,6,3,1,4,6,4,4,4,2,3,2,2,2,2,2,2,2,1,6,4/ +C SORT OUT MARGIN IE FIND MAX AND DIVIDE BY THREE + IMARG=NINT(MARGT/3.) + MARGL1=MARGL + MARGR1=MARGR + MARGB1=MARGB + MARGB2=MARGB+IMARG + MARGB3=MARGB+2*IMARG + MARGT3=IMARG + MARGT2=IMARG + MARGT1=IMARG + SD=4.1 + EXPM=0. + YMIN=EXPM + YMAX=EXPM+15.*SD + IBH=NINT(0.005*ISXMAX) + IMH=NINT(0.005*ISYMAX) +C ONLY CALC FACTORS UPTO 99! + CALL FACTAB(FTABLE,99) + I1IN=2+MXSPAN/2 + I1INM1=I1IN-1 + IDIMJ=J2-J1+1. + J1P=J1+I1INM1 + J2P=J2+I1INM1 + LB=1+LENW/2 + LB=3*LB + LF=LENW/2 + LF=3*LF + LENW3=3*LENW + XLENW=LENW + FACN=FACTOR(LENW,FTABLE,IDFTAB) + XMIN=J1 + XMAX=J2 + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL FRAME(MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + CALL FRAME(MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) + CALL SCALES(XMAX,XMIN,YMAX,YMIN,MARGL1,MARGR1, + +MARGB1,MARGT1,ISXMAX,ISYMAX,SD,IBH,YMIN,2) + CALL SCALES(XMAX,XMIN,YMAX,YMIN,MARGL1,MARGR1, + +MARGB2,MARGT2,ISXMAX,ISYMAX,SD,IBH,YMIN,2) + CALL SCALES(XMAX,XMIN,YMAX,YMIN,MARGL1,MARGR1, + +MARGB3,MARGT3,ISXMAX,ISYMAX,SD,IBH,YMIN,2) + CALL ROT2(SEQ(J1P-LB),IDIM1,NCOD1,RNFAC(1),FTABLE, + +IDFTAB,NBASE1,LENW3,FA1,FABC1,NCODON,IACID) + CALL ROT2(SEQ(J1P+1-LB),IDIM1,NCOD2,RNFAC(2),FTABLE, + +IDFTAB,NBASE2,LENW3,FA2,FABC2,NCODON,IACID) + CALL ROT2(SEQ(J1P+2-LB),IDIM1,NCOD3,RNFAC(3),FTABLE, + +IDFTAB,NBASE3,LENW3,FA3,FABC3,NCODON,IACID) +C set initial values + XP=J1 + YP1=0. + YP2=0. + YP3=0. + IDONE=0 + DO 300 IP=J1P,J2P,3 + IDONE=IDONE+1 + IP0=IP + IP1=IP+1 + IP2=IP+2 + CALL ROTFAK(SEQ,IDIM1P,IP0,LB,LF,NCOD1,RNFAC(1), + +FTABLE,IDFTAB,NBASE1,COMP(1),LENW3,XLENW,FA1,FABC1,NCODON,IACID) + CALL ROTFAK(SEQ,IDIM1P,IP1,LB,LF,NCOD2,RNFAC(2), + +FTABLE,IDFTAB,NBASE2,COMP(2),LENW3,XLENW,FA2,FABC2,NCODON,IACID) + CALL ROTFAK(SEQ,IDIM1P,IP2,LB,LF,NCOD3,RNFAC(3), + +FTABLE,IDFTAB,NBASE3,COMP(3),LENW3,XLENW,FA3,FABC3,NCODON,IACID) + DO 200 K=1,3 + W(K)=RNFAC(K)-COMP(K)-FACN +200 CONTINUE + IF(MOD(IDONE,IWRIT).EQ.0)THEN + DO 302 II=1,3 + RLINE(II)=' ' +302 CONTINUE + T=MAX(W(1),W(2),W(3)) + DO 303 II=1,3 + IF(W(II).EQ.T)RLINE(II)=STAR +303 CONTINUE + IPA=IP-I1INM1 + CALL POISON(LENW,FABC1,FABC1,EW1,WD1,2) + CALL POISON(LENW,FABC2,FABC2,EW2,WD2,2) + CALL POISON(LENW,FABC3,FABC3,EW3,WD3,2) + WDT3=(WD1+WD2+WD3)/3. + YMAX=EXPM+15.*WDT3 + WDT3=(YMAX-YMIN)/2. + X=IPA + Y1=W(1)-EW1 + Y2=W(2)-EW2 + Y3=W(3)-EW3 + CALL LINE(XP,X,YP1,Y1,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1+IMH,MARGT1-2*IMH,ISXMAX,ISYMAX) + CALL LINE(XP,X,YP2,Y2,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB2+IMH,MARGT2-2*IMH,ISXMAX,ISYMAX) + CALL LINE(XP,X,YP3,Y3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB3+IMH,MARGT3-2*IMH,ISXMAX,ISYMAX) + IF(RLINE(1).EQ.STAR)CALL POINT(X,WDT3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + IF(RLINE(2).EQ.STAR)CALL POINT(X,WDT3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + IF(RLINE(3).EQ.STAR)CALL POINT(X,WDT3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) + YP1=Y1 + YP2=Y2 + YP3=Y3 + XP=X + END IF +300 CONTINUE + CALL STARTS(SEQ,IDIM1P,J1P,J2P, + +MARGL1,MARGR1,MARGB,MARGT,ISXMAX,ISYMAX,PAA) + CALL STOPS(SEQ,IDIM1P,J1P,J2P, + +MARGL1,MARGR1,MARGB,MARGT,ISXMAX,ISYMAX,PAA) + CALL VT100M + END + SUBROUTINE ROT1(SEQ,IDIM,NCOD,RNFAC,FTABLE,IDFTAB, + +NBASE,SPAN3) +C AUTHOR: RODGER STADEN + REAL RNFAC,FTABLE(IDFTAB),FACTOR + INTEGER NCOD(4,4,4),NBASE(4),SPAN3,CTONUM + CHARACTER SEQ(IDIM) + EXTERNAL FACTOR,CTONUM +C zero values + DO 1 I=1,4 + NBASE(I)=0 + DO 1 J=1,4 + DO 1 K=1,4 +1 NCOD(I,J,K)=0 +C loop for span length + DO 10 I=1,SPAN3-1,3 + IP=I-1 +C check for bad chars + DO 5 J=1,3 +5 IF(CTONUM(SEQ(IP+J)).EQ.5)GO TO 10 +C ok + IS=CTONUM(SEQ(I)) + IS1=CTONUM(SEQ(I+1)) + IS2=CTONUM(SEQ(I+2)) + NCOD(IS,IS1,IS2)=NCOD(IS,IS1,IS2)+1 + NBASE(IS)=NBASE(IS)+1 + NBASE(IS1)=NBASE(IS1)+1 + NBASE(IS2)=NBASE(IS2)+1 +10 CONTINUE +C set up rnfac + RNFAC=0. + DO 20 I=1,4 + DO 20 J=1,4 + DO 20 K=1,4 +20 RNFAC=RNFAC+FACTOR(NCOD(I,J,K),FTABLE,IDFTAB) + END +C rotate factor + SUBROUTINE ROTFAC(SEQ,IDIM,I,LB,LF,NCOD,RNFAC,FTABLE, + +IDFTAB,NBASE,COMP,SPAN3) +C AUTHOR: RODGER STADEN + REAL RNFAC,FTABLE(IDFTAB),FACTOR + INTEGER NCOD(4,4,4),NBASE(4),SPAN3,CTONUM + CHARACTER SEQ(IDIM) + EXTERNAL FACTOR,CTONUM +C look back + IPB=I-LB-1 +C look for bad char (it will not have been used) + DO 10 J=1,3 +10 IF(CTONUM(SEQ(IPB+J)).EQ.5)GO TO 30 +C ok + IPB=IPB+1 + IS1=CTONUM(SEQ(IPB)) + IS2=CTONUM(SEQ(IPB+1)) + IS3=CTONUM(SEQ(IPB+2)) +C subtract the current factorial value from rotated score + RNFAC=RNFAC-FACTOR(NCOD(IS1,IS2,IS3),FTABLE,IDFTAB) +C subtract from codon table + NCOD(IS1,IS2,IS3)=NCOD(IS1,IS2,IS3)-1 +C add this new value to the rotated score + RNFAC=RNFAC+FACTOR(NCOD(IS1,IS2,IS3),FTABLE,IDFTAB) +C now do the composition part + NBASE(IS1)=NBASE(IS1)-1 + NBASE(IS2)=NBASE(IS2)-1 + NBASE(IS3)=NBASE(IS3)-1 +30 CONTINUE +C now look forwards + IPB=I+LF-1 +C look for bad char + DO 40 J=1,3 +40 IF(CTONUM(SEQ(IPB+J)).EQ.5)GO TO 50 +C ok + IPB=IPB+1 + IS1=CTONUM(SEQ(IPB)) + IS2=CTONUM(SEQ(IPB+1)) + IS3=CTONUM(SEQ(IPB+2)) +C subtract the current factorial value from rotated score + RNFAC=RNFAC-FACTOR(NCOD(IS1,IS2,IS3),FTABLE,IDFTAB) + NCOD(IS1,IS2,IS3)=NCOD(IS1,IS2,IS3)+1 +C add this new value to the rotated score + RNFAC=RNFAC+FACTOR(NCOD(IS1,IS2,IS3),FTABLE,IDFTAB) +C now do the composition part + NBASE(IS1)=NBASE(IS1)+1 + NBASE(IS2)=NBASE(IS2)+1 + NBASE(IS3)=NBASE(IS3)+1 +50 CONTINUE + COMP=0. + DO 60 J=1,4 + FREQ=FLOAT(NBASE(J))/FLOAT(SPAN3) + IF(FREQ.GT.0.)THEN + COMP=COMP+FLOAT(NBASE(J))*LOG(FREQ) + GO TO 60 + END IF +60 CONTINUE + END + REAL FUNCTION FACTOR(N,FTABLE,IDFTAB) +C AUTHOR: RODGER STADEN + REAL FTABLE(IDFTAB) + FACTOR=0.0 +C in range? + IF(N.LT.1)GO TO 10 + IF(N.GT.IDFTAB)GO TO 20 + FACTOR=FTABLE(N) + RETURN +10 CONTINUE + FACTOR=1. + RETURN +20 CONTINUE + WRITE(*,1000)N +1000 FORMAT(' ERROR IN FACTOR, N=',I6) + END +C factorial table set up + SUBROUTINE FACTAB(FTABLE,IDFTAB) +C AUTHOR: RODGER STADEN + REAL FTABLE(IDFTAB),X1,LOG2PI + LOG2PI=0.5*LOG(6.2832) + X1=1. + DO 10 I=1,20 + X1=X1*FLOAT(I) +10 FTABLE(I)=LOG(X1) +C stirlings formula: +C lni!=(i+.5)*lni-i+0.5ln2pi +C + DO 20 I=21,IDFTAB + X1=I +20 FTABLE(I)=(X1+0.5)*LOG(X1)-X1+LOG2PI + END + SUBROUTINE ROT2(SEQ,IDIM,NCOD,RNFAC,FTABLE,IDFTAB, + +NBASE,SPAN3,FA,FABC,NCODON,IACID) +C AUTHOR: RODGER STADEN + REAL RNFAC,FTABLE(IDFTAB),FACTOR,FA(21),FABC(4,4,4) + INTEGER NCOD(4,4,4),NBASE(4),NCODON(21),IACID(4,4,4),SPAN3 + INTEGER CTONUM + CHARACTER SEQ(IDIM) + EXTERNAL FACTOR,CTONUM + SPAN=SPAN3/3. +C zero values + DO 1 I=1,4 + NBASE(I)=0 + DO 1 J=1,4 + DO 1 K=1,4 + FABC(I,J,K)=0. +1 NCOD(I,J,K)=0 + DO 2 I=1,21 + FA(I)=0.0 +2 CONTINUE +C loop for span length + DO 10 I=1,SPAN3-1,3 + IP=I-1 +C check for bad chars + DO 5 J=1,3 +5 IF(CTONUM(SEQ(IP+J)).EQ.5)GO TO 10 +C ok + IS=CTONUM(SEQ(I)) + IS1=CTONUM(SEQ(I+1)) + IS2=CTONUM(SEQ(I+2)) + NCOD(IS,IS1,IS2)=NCOD(IS,IS1,IS2)+1 + NBASE(IS)=NBASE(IS)+1 + NBASE(IS1)=NBASE(IS1)+1 + NBASE(IS2)=NBASE(IS2)+1 +10 CONTINUE +C set up rnfac + RNFAC=0. + DO 20 I=1,4 + DO 20 J=1,4 + DO 20 K=1,4 + JACID=IACID(I,J,K) + FA(JACID)=FA(JACID)+NCOD(I,J,K) +20 RNFAC=RNFAC+FACTOR(NCOD(I,J,K),FTABLE,IDFTAB) +C CALC EVEN USE OF EACH ACIDS CODONS + DO 21 I=1,4 + DO 21 J=1,4 + DO 21 K=1,4 + JACID=IACID(I,J,K) + FABC(I,J,K)=FA(JACID)/(SPAN*NCODON(JACID)) +21 CONTINUE + END +C rotate factor + SUBROUTINE ROTFAK(SEQ,IDIM,I,LB,LF,NCOD,RNFAC,FTABLE, + +IDFTAB,NBASE,COMP,SPAN3,SPAN,FA,FABC,NCODON,IACID) +C AUTHOR: RODGER STADEN + REAL RNFAC,FTABLE(IDFTAB),FACTOR,FABC(4,4,4),FA(21) + INTEGER NCOD(4,4,4),NBASE(4),SPAN3,NCODON(21),IACID(4,4,4) + INTEGER CTONUM + CHARACTER SEQ(IDIM) + EXTERNAL FACTOR,CTONUM +C look back + IPB=I-LB-1 +C look for bad char (it will not have been used) + DO 10 J=1,3 +10 IF(CTONUM(SEQ(IPB+J)).EQ.5)GO TO 30 +C ok + IPB=IPB+1 + IS1=CTONUM(SEQ(IPB)) + IS2=CTONUM(SEQ(IPB+1)) + IS3=CTONUM(SEQ(IPB+2)) +C subtract the current factorial value from rotated score + RNFAC=RNFAC-FACTOR(NCOD(IS1,IS2,IS3),FTABLE,IDFTAB) +C subtract from codon table + NCOD(IS1,IS2,IS3)=NCOD(IS1,IS2,IS3)-1 +C add this new value to the rotated score + RNFAC=RNFAC+FACTOR(NCOD(IS1,IS2,IS3),FTABLE,IDFTAB) +C now do the composition part + NBASE(IS1)=NBASE(IS1)-1 + NBASE(IS2)=NBASE(IS2)-1 + NBASE(IS3)=NBASE(IS3)-1 + JACID=IACID(IS1,IS2,IS3) + FA(JACID)=FA(JACID)-1. + TOTA=FA(JACID)/(SPAN*NCODON(JACID)) + CALL NORMA2(FABC,JACID,TOTA) +30 CONTINUE +C now look forwards + IPB=I+LF-1 +C look for bad char + DO 40 J=1,3 +40 IF(CTONUM(SEQ(IPB+J)).EQ.5)GO TO 50 +C ok + IPB=IPB+1 + IS1=CTONUM(SEQ(IPB)) + IS2=CTONUM(SEQ(IPB+1)) + IS3=CTONUM(SEQ(IPB+2)) +C subtract the current factorial value from rotated score + RNFAC=RNFAC-FACTOR(NCOD(IS1,IS2,IS3),FTABLE,IDFTAB) + NCOD(IS1,IS2,IS3)=NCOD(IS1,IS2,IS3)+1 +C add this new value to the rotated score + RNFAC=RNFAC+FACTOR(NCOD(IS1,IS2,IS3),FTABLE,IDFTAB) +C now do the composition part + NBASE(IS1)=NBASE(IS1)+1 + NBASE(IS2)=NBASE(IS2)+1 + NBASE(IS3)=NBASE(IS3)+1 + JACID=IACID(IS1,IS2,IS3) + FA(JACID)=FA(JACID)+1. + TOTA=FA(JACID)/(SPAN*NCODON(JACID)) + CALL NORMA2(FABC,JACID,TOTA) +50 CONTINUE + COMP=0. + DO 60 J=1,4 + FREQ=FLOAT(NBASE(J))/FLOAT(SPAN3) + IF(FREQ.GT.0.)THEN + COMP=COMP+FLOAT(NBASE(J))*LOG(FREQ) + GO TO 60 + END IF +60 CONTINUE + END + SUBROUTINE POISON(LCOD,F,FABC,OUTW,OUTWD,JOB) +C AUTHOR: D. R BOSWELL AND RODGER STADEN + REAL F(4),FABC(4,4,4) +C +C modified by rs 2-2-83 to include correction of -.5*ln(2piN) where +C N is no of codons (lcod). This is required because I calc using +C multinomial distribution and poisson uses poisson distributon. +C maybe later I should also use poisson. +C Modified 25 Jan 83 to include corrections for lack of restriction +C of total codon number inherent in the Poisson model +C (see Andrew's notes of 7 Jan 83) +C +C The following arrays contain values of and <(delta W)**2> +C computed for Poisson distributions for nu from 0.0 to 10.0 in +C steps of 0.1 +C The functions are sufficiently smooth to allow linear interpolation +C between these values +C + REAL W(0:100),DW2(0:100),W10(10),DW210(10) + SAVE W10,DW2,W,DW210 + DATA W/ 0.0,0.333677, 0.535378, 0.691144, 0.819071,0.927637, + 1 1.021753, 1.104597, 1.178383, 1.244725, 1.304842, + 1 1.359685, 1.410006, 1.456414, 1.499409, 1.539405, + 1 1.576749, 1.611735, 1.644613, 1.675600, 1.704883, + 1 1.732621, 1.758958, 1.784016, 1.807907, 1.830726, + 1 1.852561, 1.873487, 1.893574, 1.912883, 1.931471, + 1 1.949385, 1.966674, 1.983377, 1.999532, 2.015173, + 1 2.030330, 2.045035, 2.059310, 2.073183, 2.086673, + 1 2.099801, 2.112587, 2.125048, 2.137200, 2.149058, + 1 2.160635, 2.171947, 2.183003, 2.193815, 2.204396, + 1 2.214752, 2.224895, 2.234834, 2.244576, 2.254128, + 1 2.263500, 2.272696, 2.281723, 2.290591, 2.299299, + 1 2.307859, 2.316271, 2.324544, 2.332680, 2.340684, + 1 2.348562, 2.356317, 2.363950, 2.371470, 2.378875, + 1 2.386173, 2.393366, 2.400454, 2.407444, 2.414335, + 1 2.421134, 2.427840, 2.434457, 2.440988, 2.447433, + 1 2.453796, 2.460078, 2.466282, 2.472411, 2.478464, + 1 2.484445, 2.490354, 2.496194, 2.501966, 2.507673, + 1 2.513316, 2.518894, 2.524412, 2.529868, 2.535266, + 1 2.540605, 2.545888, 2.551117, 2.556289, 2.561411/ + DATA DW2/ 0.0,0.564147, 0.615536, 0.604980,0.578119,0.548425, + 1 0.520633, 0.496362, 0.475974, 0.459312, 0.446008, + 1 0.435632, 0.427759, 0.421991, 0.417978, 0.415411, + 1 0.414025, 0.413597, 0.413938, 0.414884, 0.416304, + 1 0.418091, 0.420146, 0.422401, 0.424790, 0.427265, + 1 0.429780, 0.432308, 0.434822, 0.437301, 0.439725, + 1 0.442090, 0.444380, 0.446592, 0.448719, 0.450763, + 1 0.452725, 0.454593, 0.456378, 0.458077, 0.459696, + 1 0.461236, 0.462698, 0.464085, 0.465401, 0.466650, + 1 0.467835, 0.468958, 0.470021, 0.471033, 0.471987, + 1 0.472895, 0.473758, 0.474571, 0.475347, 0.476081, + 1 0.476778, 0.477444, 0.478074, 0.478669, 0.479243, + 1 0.479780, 0.480300, 0.480791, 0.481261, 0.481711, + 1 0.482136, 0.482542, 0.482935, 0.483305, 0.483668, + 1 0.484009, 0.484333, 0.484652, 0.484950, 0.485244, + 1 0.485518, 0.485790, 0.486046, 0.486291, 0.486531, + 1 0.486763, 0.486985, 0.487203, 0.487408, 0.487606, + 1 0.487800, 0.487990, 0.488172, 0.488348, 0.488517, + 1 0.488678, 0.488844, 0.488993, 0.489148, 0.489290, + 1 0.489438, 0.489575, 0.489704, 0.489840, 0.489961/ +C +C The next two arrays are for nu from 10.0 to 100.0 in steps of 10.0 +C + DATA W10/ + 1 2.561411, 2.912526, 3.116712, 3.261270, 3.373267, + 1 3.464711, 3.541987, 3.608907, 3.667911, 3.720688/ + DATA DW210/ + 1 0.489961, 0.495480, 0.497065, 0.497826, 0.498281, + 1 0.498570, 0.498778, 0.498917, 0.499063, 0.499146/ +C +C rs correction factor + CORREC=0.5*LOG(6.2832*LCOD) +C +C +C Initialise accumulators to zero +C (Note that SDW will accumulate the sum of squares of DW, and +C we will need to take its square root at the end +C + SW=0.0 + SDW=0.0 +C Step through all 64 codons + DO 200 I=1,4 + DO 200 J=1,4 + DO 200 K=1,4 +C V (=nu) is the expected codon frequency in this slot of the table + IF(JOB.EQ.1)THEN + V=REAL(LCOD)*F(I)*F(J)*F(K) + ELSE IF(JOB.EQ.2)THEN + V=REAL(LCOD)*FABC(I,J,K) + END IF +C V<0.0 is a fatal error (implies bad parameters) + IF(V.LT.0.0)GO TO 900 +C V>=10.0 requires separate handling + IF(V.GE.10.0)GOTO 100 +C Now we can interpolate in the tables and accumulate the appropriate values + IV=IFIX(V*10.0) + DV=V*10.0-IV + SW=SW+W(IV)+DV*(W(IV+1)-W(IV)) + SDW=SDW+DW2(IV)+DV*(DW2(IV+1)-DW2(IV)) + GO TO 200 +C Come here if V>=10.0 +100 IF(V.GT.100.0)GOTO 110 + IV=IFIX(V/10.0) + DV=V/10.0-IV + SW=SW+W10(IV)+DV*(W10(IV+1)-W10(IV)) + SDW=SDW+DW210(IV)+DV*(DW210(IV+1)-DW210(IV)) + GO TO 200 +C If V>100.0 we're quite justified in using the "large V" approximations +110 SW=SW+0.5*(LOG(2.0*3.1415926536)+LOG(V)+1.0) + SDW=SDW+0.5 +200 CONTINUE +C Include correction factors + OUTW=MAX(0.0,SW-64.0/(4.0*REAL(LCOD))) + OUTWD=SQRT(MAX(0.0,SDW-64.0*64.0/(4.0*REAL(LCOD)))) +C rs correction + OUTW=OUTW-CORREC + RETURN +C Come here if there's an error in the parameters +900 WRITE(*,1)LCOD,F +1 FORMAT(' POISSON ERROR -- INVALID PARAMETERS'/, + 1' SEQUENCE LENGTH: ',I5,' BASE FREQUENCIES: ',4F8.3) + OUTW=0.0 + OUTWD=0.0 + END + SUBROUTINE NORMA2(SUM,JACID,TOTA) +C AUTHOR: RODGER STADEN +C routine to set all the values for any acid to one +C particular value=tota for a table of codon freqs +C ncods is number of cods per acid numbering from f to g +C codnos are pointers to the values of codon usage in sum +C (which is 4,4,4) for each set of codons for each acid in +C turn from f to g (* is acid 11) +C codnos are pointers to the first element in codp for each +C acids set of codons +C if jacid gt 0 process only acid jacid, else process all +C tota is the total value each acid should sum to + REAL SUM(64) + INTEGER NCODS(21),CODNOS(21),CODP(64) + SAVE NCODS,CODNOS,CODP + DATA NCODS/2,6,3,1,4,6,4,4,4,2,3,2,2,2,2,2,2,2,1,6,4/ + DATA CODNOS/1,3,9,12,13,17,23,27,31,35,37,40,42,44,46, + +48,50,52,54,55,61/ + DATA CODP/1,17,33,49,2,18,34,50,3,19,35,51, + +4,20,36,52,5,21,37,53,15,31,6,22,38,54, + +7,23,39,55,8,24,40,56,9,25,41,57,45, + +10,26,42,58,11,27,43,59,12,28,44,60, + +13,29,61,14,30,46,62,47,63,16,32,48,64/ + IACID=1 + IF(JACID.NE.0)IACID=JACID +1 CONTINUE +C loop for acid iacid from codnos to codnos+ncods-1 + DO 20 J=CODNOS(IACID),CODNOS(IACID)+NCODS(IACID)-1 + SUM(CODP(J))=TOTA +20 CONTINUE + IF(JACID.NE.0)RETURN + IACID=IACID+1 + IF(IACID.LT.22)GO TO 1 + END + SUBROUTINE PCODUS(SEQ,IDIM1P,IEND,MXSPAN, + +ISXMAX,ISYMAX,J1,J2,KSTART,MARGL,MARGR,MARGB,MARGT,IDEV,FILNAM, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PAA) + CHARACTER HELPF*(*),PAA(5,5,5) +C AUTHOR: RODGER STADEN + REAL FREQ(64),SUM(64),LOGFRQ(64) + CHARACTER SEQ(IDIM1P),FILNAM*(*) + INTEGER ANSTY +C NOTE J1 J2 IS THE ACTIVE REGION, KSTART IS THE NUMBER OF THE +C BASE IN ELEMENT 1 OF SEQ, AND IEND IS THE SEQUENCE NUMBER OF THELAST +C ELEMENT IN THE RAM BUFFER +C PLOTS ARE FROM J1 TO J2 BUT STANDARDS CAN +C BE TAKEN FROM KSTART TO IEND + CALL SHOWFU(KBOUT,'Staden and McLachlan codon usage') +C WRITE(KBOUT,*)'Staden and McLachlan codon usage method' +C WRITE(KBOUT,*)'Codon tables for standards may be read from disk' +C WRITE(KBOUT,*)'or calculated from parts of the current sequence' + CALL GETPAR(42,7,IOK,MINSP,MAXSP,LENW,MINIW,MAXIW,IWRIT,ANSTY, + +IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + CALL PCODD(SEQ,IDIM1P,IEND,MXSPAN, + +KSTART,IDEV,FILNAM,FREQ,SUM,LOGFRQ,ANSTY, + +MINSP,MAXSP,LENW,MINIW,MAXIW,IWRIT,P1,P2,P3, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PAA,IOK) + IF(IOK.NE.0) RETURN + CALL PCODPP(SEQ,IDIM1P,MXSPAN, + +ISXMAX,ISYMAX,J1,J2,KSTART,MARGL,MARGR,MARGB,MARGT,PAA, + +LOGFRQ,LENW,IWRIT,P1,P2,P3) + END + SUBROUTINE PCODD(SEQ,IDIM1P,IEND,MXSPAN, + +KSTART,IDEV,FILNAM,FREQ,SUM,LOGFRQ,ANSTY, + +MINSP,MAXSP,LENW,MINIW,MAXIW,IWRIT,P1,P2,P3, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PAA,IOK) + CHARACTER HELPF*(*),PAA(5,5,5) +C AUTHOR: RODGER STADEN + REAL FREQ(64),SUM(64),LOGFRQ(64) + CHARACTER SEQ(IDIM1P),FILNAM*(*) + INTEGER ANSTY + CALL FILLR(FREQ,64,0.) + CALL PCODD1(ANSTY,PAA,FREQ,IDEV,FILNAM,IOK, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOK.NE.0) RETURN + I1INM1 = 1+(MXSPAN/2)-KSTART+1 + IF(ANSTY.EQ.0) THEN +6 CONTINUE + I1 = 0 + I2 = 0 + CALL GTREG(KBIN,KBOUT,0,IEND,I1,I2, + + 'Define standard', + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF(I1.NE.0) THEN + IDIM=I2-I1+1 + IF(IDIM.GT.3) THEN + CALL FILLR(SUM,64,0.) + CALL CALCOD(SUM,SEQ(I1+I1INM1),IDIM) + CALL ADDR(SUM,FREQ,64) + CALL WRTCOD(SUM,KBOUT,PAA) + GO TO 6 + END IF + END IF + END IF + SUMCOD=0. + DO 50 I=1,64 + SUMCOD=SUMCOD+FREQ(I) +50 CONTINUE + WRITE(KBOUT,1006)SUMCOD +1006 FORMAT(' Total codons in standard= ',F8.0) +C DONT CONTINUE IF LESS THAN 64. CODONS SPECIFIED + IF(SUMCOD.LT.64.)THEN + CALL ERROM(KBOUT,'At least 64 codons required in standard') + IOK = 1 + RETURN + END IF + CALL AACODM(KBIN,KBOUT,FREQ,PAA,SUMCOD, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.LT.0)RETURN + CALL WRTCOD(FREQ,KBOUT,PAA) + SUMCOD=0. + DO 51 I=1,64 + SUMCOD=SUMCOD+FREQ(I) +51 CONTINUE +C NORMALIZE, DO STOP CODONS AND ZERO CODONS + CALL PCOD2(FREQ,LOGFRQ,SUMCOD,LOGFRQ,PAA) +C CALL WRTCOD(LOGFRQ,KBOUT,PAA) +C CALC EXPECTED FREQS FRAME S AND T CODONS AND EXPECTED P'S FOR ALL FRAMES + LENWT = 11 + DO 60 I = 1,3 + LENWT = LENWT + 10 + CALL PCOD3(FREQ,LOGFRQ,LENWT,P1,P2,P3) + WRITE(KBOUT,2000)LENWT,P1,P2,P3 +60 CONTINUE +2000 FORMAT(' Span length ',I3,' expected mean values:',3(F6.1)) + CALL GSPIN(MINSP,MAXSP,LENW,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL PCOD3(FREQ,LOGFRQ,LENW,P1,P2,P3) + END + SUBROUTINE PCODPP(SEQ,IDIM1P,MXSPAN, + +ISXMAX,ISYMAX,J1,J2,KSTART,MARGL,MARGR,MARGB,MARGT,PAA, + +LOGFRQ,LENW,IWRIT,P1,P2,P3) + CHARACTER PAA(5,5,5) +C AUTHOR: RODGER STADEN + REAL LOGFRQ(64),SUMW(3) + CHARACTER SEQ(IDIM1P),RFRAME(3) + LB=1+LENW/2 + LB=3*LB + LF=LENW/2 + LF=3*LF + LENW3=3*LENW + EPS=0.0000001 + IMARG=NINT(MARGT/3.) + MARGL1=MARGL + MARGR1=MARGR + MARGB1=MARGB + MARGB2=MARGB+IMARG + MARGB3=MARGB+2*IMARG + MARGT3=IMARG + MARGT2=IMARG + MARGT1=IMARG + I1IN=2+(MXSPAN/2)-KSTART+1 + I1INM1=I1IN-1 + IDIMJ=J2-J1+1. + J1P=J1+I1INM1 + J2P=J2+I1INM1 + XMIN=J1 + XMAX=J2 + IBH=NINT(0.005*ISXMAX) + IMH=NINT(0.005*ISYMAX) + SD=1. + CALL FILLR(SUMW,3,0.) + IDONE=0 + YMIN=MIN(P2,P3) + YMIN=YMIN + YMAX=P1 + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL FRAME(MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + CALL FRAME(MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) + YFIRST=INT(YMIN) + CALL SCALES(XMAX,XMIN,YMAX,YMIN,MARGL1,MARGR1, + +MARGB1,MARGT1,ISXMAX,ISYMAX,SD,IBH,YFIRST,2) + CALL SCALES(XMAX,XMIN,YMAX,YMIN,MARGL1,MARGR1, + +MARGB2,MARGT2,ISXMAX,ISYMAX,SD,IBH,YFIRST,2) + CALL SCALES(XMAX,XMIN,YMAX,YMIN,MARGL1,MARGR1, + +MARGB3,MARGT3,ISXMAX,ISYMAX,SD,IBH,YFIRST,2) + CALL CODSTR(SEQ(J1P-LB),IDIM1P,LOGFRQ,LENW3,SUMW(1)) + CALL CODSTR(SEQ(J1P+1-LB),IDIM1P,LOGFRQ,LENW3,SUMW(2)) + CALL CODSTR(SEQ(J1P+2-LB),IDIM1P,LOGFRQ,LENW3,SUMW(3)) + XP=J1 + YP1=0. + YP2=0. + YP3=0. + DO 300 IP=J1P,J2P,3 + IDONE=IDONE+1 + IP0=IP + IP1=IP+1 + IP2=IP+2 + CALL ROTCOD(SEQ,IDIM1P,IP0,LB,LF,LOGFRQ,SUMW(1)) + CALL ROTCOD(SEQ,IDIM1P,IP1,LB,LF,LOGFRQ,SUMW(2)) + CALL ROTCOD(SEQ,IDIM1P,IP2,LB,LF,LOGFRQ,SUMW(3)) + IF(MOD(IDONE,IWRIT).EQ.0)THEN + CALL PCOD1(SUMW,Y1,Y2,Y3,RFRAME) +C CHECK FOR OVERFLOW SHOWN BY VALUES =0. + IF(ABS(Y1).LT.EPS)Y1=YP1 + IF(ABS(Y2).LT.EPS)Y2=YP2 + IF(ABS(Y3).LT.EPS)Y3=YP3 + IPA=IP-I1INM1 + X=IPA + CALL LINE(XP,X,YP1,Y1,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1+IMH,MARGT1-2*IMH,ISXMAX,ISYMAX) + CALL LINE(XP,X,YP2,Y2,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB2+IMH,MARGT2-2*IMH,ISXMAX,ISYMAX) + CALL LINE(XP,X,YP3,Y3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB3+IMH,MARGT3-2*IMH,ISXMAX,ISYMAX) + IF(RFRAME(1).EQ.'*') + + CALL POINT(X,0.,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + IF(RFRAME(2).EQ.'*') + + CALL POINT(X,0.,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + IF(RFRAME(3).EQ.'*') + + CALL POINT(X,0.,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) +C SAVE CURRENT VALUES + YP1=Y1 + YP2=Y2 + YP3=Y3 + XP=X + END IF +300 CONTINUE +C STARTS + CALL STARTS(SEQ,IDIM1P,J1P,J2P, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,PAA) +C STOPS + CALL STOPS(SEQ,IDIM1P,J1P,J2P, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,PAA) + CALL VT100M + END + SUBROUTINE PCODD1(ANSTY,PAA,FREQ,IDEV,FILNAM,IOK, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + REAL FREQ(64) + CHARACTER PAA(125),HELPF*(*),FILNAM*(*) + INTEGER CHOICE,ANSTY + IOK = 1 + CHOICE = ANSTY + CALL YESONO(CHOICE,'Define internal standard', + +'Read standard from file', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(CHOICE.LT.0) RETURN + ANSTY = CHOICE + IF(ANSTY.EQ.1) THEN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + + 'File name of standard', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL REDCOD(FREQ,IDEV) + CLOSE(UNIT=IDEV) + CALL WRTCOD(FREQ,KBOUT,PAA) + END IF + IOK = 0 + END + SUBROUTINE AACODM(KBIN,KBOUT,FREQ,PAA,SUMCOD, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + REAL FREQ(64) + CHARACTER PAA(5,5,5) + CHARACTER HELPF*(*) + INTEGER CHOICE + PARAMETER (MAXPRM = 43) + CHARACTER PROMPT(3)*(MAXPRM) + CHOICE = 1 + PROMPT(1) = 'Use observed frequencies' + PROMPT(2) = 'Normalize to average amino acid composition' + PROMPT(3) = 'Normalize to no amino acid bias' + CALL RADION('Select normalization',PROMPT,3,CHOICE, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(CHOICE.LT.1) THEN + IOK = 1 + RETURN + END IF + SUMC = SUMCOD/64. + IF(CHOICE.EQ.2) CALL APLAAC(FREQ,PAA) + IF(CHOICE.EQ.3) CALL NORMAA(FREQ,SUMC,PAA) + IOK = 0 + END + SUBROUTINE APLAAC(SUM,PAA) + CHARACTER PAA(5,5,5),AA(21) + REAL SUM(4,4,4),EXPERC(21) +C ARGOS VALUES + SAVE EXPERC,AA + DATA EXPERC/8.3,1.7,5.3,6.2,3.9,7.2,2.2,5.2,5.7, + +9.0,2.4,4.4,5.1,4.0,5.7,6.9,5.8,6.6,1.3,3.2,0.0/ + DATA AA/'A','C','D','E','F', + 1'G','H','I','K','L', + 2'M','N','P','Q','R', + 3'S','T','V','W','Y','*'/ +C LOOP FOR EACH ACID + DO 150 L=1,21 + ASUM=0. +C LOOP FOR EACH CODON + DO 200 I=1,4 + DO 200 J=1,4 + DO 200 K=1,4 +C COUNT CODONS + IF(PAA(K,J,I).EQ.AA(L))ASUM=ASUM+SUM(I,J,K) +200 CONTINUE + IF(ASUM.GT.0.0)ASUM = 6.4 / ASUM +C LOOP FOR EACH CODON + DO 100 I=1,4 + DO 100 J=1,4 + DO 100 K=1,4 +C NORMALIZE + IF(PAA(K,J,I).EQ.AA(L))SUM(I,J,K)=SUM(I,J,K)*ASUM*EXPERC(L) +100 CONTINUE +150 CONTINUE + END + SUBROUTINE PCOD3(FREQ,LOGFRQ,LENW,P11,P22,P33) +C AUTHOR: RODGER STADEN + PARAMETER (SMALL = 1.0E-30) + DOUBLE PRECISION U,V,W,SL,UL,VL,WL,P1,P2,P3 + REAL FREQ(64),LOGFRQ(64),S2(64),S3(64) + CALL F2FF1(FREQ,S2) + CALL F3FF1(FREQ,S3) +C CALC EXPECTED MEAN SCORES FOR THIS WINDOW LENGTH AND STANDARD + U=0. + V=0. + W=0. + DO 62 I=1,64 + U=U+FREQ(I)*LOGFRQ(I) + V=V+S2(I)*LOGFRQ(I) + W=W+S3(I)*LOGFRQ(I) +62 CONTINUE + SL=U+V+W + SL=SL/3. + U=U-SL + V=V-SL + W=W-SL + UL=EXP(U*LENW) + VL=EXP(V*LENW) + WL=EXP(W*LENW) + SL=UL+VL+WL + P1=UL/SL + P2=VL/SL + P3=WL/SL + IF(ABS(P1-1.0).GT.SMALL)P11=LOG10(P1/(1.-P1)) + IF(ABS(P2-1.0).GT.SMALL)P22=LOG10(P2/(1.-P2)) + IF(ABS(P3-1.0).GT.SMALL)P33=LOG10(P3/(1.-P3)) + END + SUBROUTINE PCOD2(FREQ,LOGFRQ,SUMCOD,LOGFR3,PAA) +C AUTHOR: RODGER STADEN + CHARACTER PAA(5,5,5) + REAL FREQ(64),LOGFRQ(64),LOGFR3(4,4,4) +C NORMALIZE + DO 51 I=1,64 + FREQ(I)=FREQ(I)/SUMCOD +51 CONTINUE +C TAKE LOGS + DO 52 I=1,64 + LOGFRQ(I)=0.0 + IF(FREQ(I).NE.0.)LOGFRQ(I)=LOG(FREQ(I)) +52 CONTINUE +C NEED TO DEAL WITH STOP CODONS AND ZERO FREQS + ODX=1./SUMCOD + ODXL=LOG(ODX) + STOPT=0. + NSTOP=0 +C LOOK FOR STOPS IN PAA + DO 10 I=1,4 + DO 10 J=1,4 + DO 10 K=1,4 + IF(PAA(K,J,I).EQ.'*')THEN + LOGFR3(I,J,K)=99. + STOPT=STOPT+99. + NSTOP=NSTOP+1 + END IF +10 CONTINUE +C SET ZEROES TO LOG(1/TOTAL) + DO 53 I=1,64 + IF(LOGFRQ(I).EQ.0.)LOGFRQ(I)=ODXL +53 CONTINUE +C SET STOPS TO MEAN + X1=0. + DO 54 I=1,64 + X1=X1+LOGFRQ(I) +54 CONTINUE + IF(NSTOP.NE.64)X1=(X1-STOPT)/(64-NSTOP) +C LOOK FOR STOPS IN PAA + DO 20 I=1,4 + DO 20 J=1,4 + DO 20 K=1,4 + IF(PAA(K,J,I).EQ.'*')LOGFR3(I,J,K)=X1 +20 CONTINUE + END + SUBROUTINE PCOD1(SUMW,Y1,Y2,Y3,LINE) +C AUTHOR: RODGER STADEN + DOUBLE PRECISION SUMWT(3),T,T1 + REAL SUMW(3) + CHARACTER LINE(3) +C calc mean of sums to add to each + T=SUMW(1)+SUMW(2)+SUMW(3) + T=T/3. + T=-1.*T + DO 80 II=1,3 +C add mean to each + SUMWT(II)=SUMW(II)+T +C calc exponential of each + SUMWT(II)=EXP(SUMWT(II)) +80 CONTINUE +C calc sum of exponentials + T=SUMWT(1)+SUMWT(2)+SUMWT(3) + DO 298 II=1,3 +C divide each by sum of exponetials + IF(T.NE.0.)SUMWT(II)=SUMWT(II)/T + T1=1.-SUMWT(II) + IF(T1.NE.0.)SUMWT(II)=SUMWT(II)/T1 + IF(SUMWT(II).GT.0.)SUMWT(II)=LOG10(SUMWT(II)) +298 CONTINUE +C get largest + CALL FILLC(LINE,3,' ') + T=MAX(SUMWT(1),SUMWT(2),SUMWT(3)) + DO 10 I=1,3 + IF(T.EQ.SUMWT(I))LINE(I)='*' +10 CONTINUE + Y1=SUMWT(1) + Y2=SUMWT(2) + Y3=SUMWT(3) + END + SUBROUTINE CODSTR(SEQ,IDIM,LOGFRQ,LWIND3,SUM) +C AUTHOR: RODGER STADEN + REAL LOGFRQ(4,4,4) + CHARACTER SEQ(IDIM) + INTEGER CTONUM,IVAL(3) + EXTERNAL CTONUM + SUM=0. +C LOOP FOR WINDOW LENGTH + DO 100 I=1,LWIND3-1,3 + IP=I-1 +C CHECK FOR NULL CHARS + DO 10 J=1,3 + IVAL(J)=CTONUM(SEQ(IP+J)) + IF(IVAL(J).EQ.5)GO TO 100 +10 CONTINUE + SUM=SUM+LOGFRQ(IVAL(1),IVAL(2),IVAL(3)) +100 CONTINUE + END + SUBROUTINE F2FF1(SUM1,SUM2) +C AUTHOR: RODGER STADEN + REAL SUM1(4,4,4),SUM2(4,4,4),SXAB(4,4),SCXX(4) + DO 1 I=1,4 + DO 1 J=1,4 + DO 1 K=1,4 + SUM2(K,J,I)=0. +1 CONTINUE +C CALC FROM XABCXX ABC IE XAB * CXX +C XAB + DO 10 J=1,4 + DO 10 K=1,4 + SXAB(J,K)=0. + DO 10 I=1,4 + SXAB(J,K)=SXAB(J,K)+SUM1(I,J,K) +10 CONTINUE +C CXX + DO 20 I=1,4 + SCXX(I)=0. + DO 20 J=1,4 + DO 20 K=1,4 + SCXX(I)=SCXX(I)+SUM1(I,J,K) +20 CONTINUE +C SUM2= XAB * CXX + DO 30 I=1,4 + DO 30 J=1,4 + DO 30 K=1,4 + SUM2(I,J,K)=SXAB(I,J)*SCXX(K) +30 CONTINUE + END +C F3FF1 + SUBROUTINE F3FF1(SUM1,SUM3) +C AUTHOR: RODGER STADEN + REAL SUM1(4,4,4),SUM3(4,4,4),SXXA(4),SBCX(4,4) + DO 1 I=1,4 + DO 1 J=1,4 + DO 1 K=1,4 + SUM3(K,J,I)=0. +1 CONTINUE +C CALC ABC FROM XXABCXX IE XXA * BCX +C XXA + DO 10 K=1,4 + SXXA(K)=0. + DO 10 I=1,4 + DO 10 J=1,4 + SXXA(K)=SXXA(K)+SUM1(I,J,K) +10 CONTINUE +C BCX + DO 20 I=1,4 + DO 20 J=1,4 + SBCX(I,J)=0. + DO 20 K=1,4 + SBCX(I,J)=SBCX(I,J)+SUM1(I,J,K) +20 CONTINUE +C XXA * BCX + DO 30 I=1,4 + DO 30 J=1,4 + DO 30 K=1,4 + SUM3(I,J,K)=SXXA(I)*SBCX(J,K) +30 CONTINUE + END +C THIS ROUTINE DOES NOT CHECK FOR GOING OUT OF ARRAY + SUBROUTINE ROTCOD(SEQ,IDIM1,I,LB,LF,LOGFRQ,H) +C AUTHOR: RODGER STADEN + REAL LOGFRQ(4,4,4) + CHARACTER SEQ(IDIM1) + INTEGER CTONUM,IVAL(3) + EXTERNAL CTONUM + XS=0. + XA=0. +C LOOK BACK + IPB=I-LB-1 + DO 10 J=1,3 + IVAL(J)=CTONUM(SEQ(IPB+J)) + IF(IVAL(J).EQ.5)GO TO 30 +10 CONTINUE +C SET VALUE TO SUBTRACT + XS=LOGFRQ(IVAL(1),IVAL(2),IVAL(3)) +C NOW LOOK FORWARD +30 CONTINUE + IPB=I+LF-1 + DO 45 J=1,3 + IVAL(J)=CTONUM(SEQ(IPB+J)) + IF(IVAL(J).EQ.5)GO TO 50 +45 CONTINUE +C OK SO USE + XA=LOGFRQ(IVAL(1),IVAL(2),IVAL(3)) +50 H=H-XS+XA + RETURN + END +C PSRCH + SUBROUTINE PSRCH(SEQ,IDIM1,J1,J2,STRING,IDIMS,INC, + +XMAX,XMIN,YMAX,YMIN,MARGL,MARGR,MARGB,MARGT,ISXMAX, + +ISYMAX,Y,BH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIMS) + YP=Y+BH + IAT=J1-INC +10 CONTINUE + IAT=IAT+INC + IDIM=J2-IAT + IF(IDIM.GT.0)THEN + CALL FIND6(SEQ(IAT),IDIM,STRING,IDIMS,INC,JMATCH) + IF(JMATCH.NE.0)THEN + IAT=IAT+JMATCH-1 + X=IAT + CALL LINE(X,X,YP,Y,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + GO TO 10 + END IF + END IF + END + SUBROUTINE PBASEP(SEQ,IDIM1P,MXSPAN, + +ISXMAX,ISYMAX,J1,J2,KSTART,IENDB, + +MARGL1,MARGR1,MARGB,MARGT,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PAA,IDEV,FILNAM,DIALOG) + CHARACTER HELPF*(*),PAA(5,5,5),FILNAM*(*) +C AUTHOR: RODGER STADEN +C 11-1-91 Added facility to read standard in the form of codon table +C and greatly reorganised code! and added new routines. + REAL EXPEC(5,3),SCORES(5,3),WT(3),S(3) + CHARACTER SEQ(IDIM1P) + INTEGER ANSTY,ANSABS,ANSS,DIALOG +C ARGOS VALUES + SAVE EXPEC + DATA EXPEC/0.1768,0.2108,0.2767,0.3357,0., + + 0.2707,0.2378,0.3097,0.1818,0., + + 0.2506,0.2506,0.2396,0.2592,0./ +C ORIGINAL DAYHOFF VALUES +C DATA EXPEC/0.1835,0.1932,0.2719,0.3514,0., +C 10.2382,0.2459,0.3173,0.1985,0., +C 20.2548,0.2548,0.2377,0.2527,0./ +C NOTE J1 J2 IS THE ACTIVE REGION, KSTART IS THE NUMBER OF THE +C BASE IN ELEMENT 1 OF SEQ, AND IDIM1 IS THE LENGTH FROM KSTART +C TO THE END OF SEQ. PLOTS ARE FROM J1 TO J2 BUT STANDARDS CAN +C BE TAKEN FROM KSTART TO KSTART+IDIM1-1 = IENDB + CALL SHOWFU(KBOUT, + +'Positional base preferences method to find protein genes') + CALL GETPAR(43,9,IOK,MINSP,MAXSP,LENW,MINIW,MAXIW,IWRIT, + +ANSABS,ANSTY,ANSS, + +IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + CALL PBASPD(SEQ,IDIM1P,MXSPAN, + +KSTART,IENDB,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH, + +ANSTY,ANSABS,ANSS,MINSP,MAXSP,LENW,MINIW,MAXIW,IWRIT, + +EXPEC,SCORES,WT,S,SMIN,SMAX,DIALOG,PAA,IDEV,FILNAM,IOK) + IF(IOK.NE.0) RETURN + CALL PBASPP(SEQ,IDIM1P,MXSPAN, + +ISXMAX,ISYMAX,J1,J2,KSTART,ANSABS,LENW,IWRIT, + +EXPEC,SCORES,WT,S,SMIN,SMAX, + +MARGL1,MARGR1,MARGB,MARGT,PAA) + END + SUBROUTINE PBASPD(SEQ,IDIM1P,MXSPAN, + +KSTART,IENDB,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH, + +ANSTY,ANSABS,ANSS,MINSP,MAXSP,LENW,MINIW,MAXIW,IWRIT, + +EXPEC,SCORES,WT,S,SMIN,SMAX,DIALOG,PAA,IDEV,FILNAM,IOK) +C AUTHOR: RODGER STADEN + REAL EXPEC(5,3),SCORES(5,3),WT(3),S(3) + CHARACTER SEQ(IDIM1P) + INTEGER ANSTY,ANSABS,ANSS,CHOICE,DIALOG + REAL FREQ(64) + CHARACTER PAA(125),HELPF*(*),FILNAM*(*) + PARAMETER (MAXPRM = 28) + CHARACTER PROMPT(3)*(MAXPRM) + IOK = 1 + DO 30 J=1,3 + WT(J)=1.0 + DO 30 I=1,5 + SCORES(I,J)=EXPEC(I,J) +30 CONTINUE + IF(DIALOG.EQ.0) THEN + CALL PBEXP(SCORES,WT,S) + CALL PBSCAL(SMIN,SMAX,S,ANSABS,LENW,SMIN5,SMAX5,DIF) + IOK = 0 + RETURN + END IF +C +C Select source of standard +C + PROMPT(1) = 'Use global standard' + PROMPT(2) = 'Use internal standard' + PROMPT(3) = 'Use codon usage table' + IOK = 1 + CHOICE = 1 + CALL RADION('Select standard source',PROMPT,3,CHOICE, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(CHOICE.LT.1) RETURN + ANSTY = CHOICE + IF(ANSTY.EQ.3) THEN +C +C Get standard from a codon table +C + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + + 'File name of standard', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL REDCOD(FREQ,IDEV) + CLOSE(UNIT=IDEV) + CALL WRTCOD(FREQ,KBOUT,PAA) + CALL GETPBS(FREQ,SCORES) + ELSE IF(ANSTY.EQ.2) THEN +C +C Get standard from a section of the sequence +C + CALL PBD1(SEQ,IDIM1P,MXSPAN,KSTART,IENDB,KBIN,KBOUT, + + SCORES,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + IF(ANSTY.NE.1) THEN +C +C Allow combination with global standard +C + PROMPT(1) = 'Use observed frequencies' + PROMPT(2) = 'Combine with global standard' + IOK = 1 + CHOICE = 1 + CALL RADION('Select normalisation',PROMPT,2,CHOICE, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(CHOICE.LT.1) RETURN + IF(CHOICE.EQ.2)THEN + DO 36 I=1,2 + DO 36 K=1,4 + SCORES(K,I)=EXPEC(K,I) +36 CONTINUE + END IF + END IF + WRITE(KBOUT,1004) +1004 FORMAT(' T C A G Range') + DO 4 I=1,3 + BIG=0.0 + SMALL=99999.0 + DO 5 K=1,4 + BIG = MAX(SCORES(K,I),BIG) + SMALL = MIN(SCORES(K,I),SMALL) +5 CONTINUE + RANGE=BIG-SMALL + WRITE(KBOUT,1005)I,(SCORES(K,I),K=1,4),RANGE +1005 FORMAT(' ',I3,4(1X,F6.3),1X,F6.3) +4 CONTINUE +C +C Allow different weights for each position +C + IOK = 1 + CHOICE = 0 + CALL YESONO(CHOICE, + +'Use 1.0 for positional weights', + +'Change positional weights', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(CHOICE.LT.0) RETURN + IOK = 0 + IF(CHOICE.EQ.1) CALL PBPD2(WT,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL PBEXP(SCORES,WT,S) + WRITE(KBOUT,1001)S +1001 FORMAT( + + ' Expected scores per codon in each frame',/,' ',3F10.3) + CALL GSPIN(MINSP,MAXSP,LENW,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN +C +C Select relative or absolute values +C + IOK = 1 + CHOICE = ANSABS + CALL YESONO(CHOICE, + + 'Plot relative scores', + + 'Plot absolute scores', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(CHOICE.LT.0) RETURN + ANSABS = CHOICE + CALL PBSCAL(SMIN,SMAX,S,ANSABS,LENW,SMIN5,SMAX5,DIF) + WRITE(KBOUT,1002)SMIN,SMAX,DIF +1002 FORMAT(' Scaling values:',/, + +' Minimum maximum range',/,2X,3F9.4) +C +C Allow plot scaling +C + IOK = 1 + CHOICE = ANSS + CALL YESONO(CHOICE, + +'Leave scaling values unchanged', + +'Change scaling values', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(CHOICE.LT.0) RETURN + ANSS = CHOICE + IF(ANSS.EQ.1) THEN + CALL GETRL(SMIN5,SMAX5,SMIN,'Minimum',VALUE, + + KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + SMIN = VALUE + CALL GETRL(SMIN,SMAX5,SMAX,'Maximum',VALUE, + + KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + SMAX = VALUE + END IF + IOK = 0 + END + SUBROUTINE PBSCAL(SMIN,SMAX,S,ANSABS,LENW,SMIN5,SMAX5,DIF) +C AUTHOR: RODGER STADEN + REAL S(3) + INTEGER ANSABS + SM=0. + IF(ANSABS.EQ.0) THEN + DO 25 I=1,3 + SM=SM+S(I) +25 CONTINUE + DO 26 I=1,3 + S(I)=S(I)/SM +26 CONTINUE + ELSE + DO 27 I=1,3 + S(I)=S(I)*LENW +27 CONTINUE + END IF + SMAX=MAX(S(1),S(2),S(3)) + SMIN=MIN(S(1),S(2),S(3)) + DIF=SMAX-SMIN + D20=DIF*0.2 + SMAX=SMAX+D20 + SMIN=SMIN-D20 + D50 = DIF*0.5 + SMAX5 = SMAX + D50 + SMIN5 = SMIN - D50 + END + SUBROUTINE PBPD2(WT,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + REAL WT(3) + CHARACTER HELPF*(*) + WRITE(KBOUT,1000) +1000 FORMAT(' Give weights between 0.0 and 1.0',/, + + ' to each of the 3 codon positions') + WMIN = 0. + WMAX = 1. + DEF = 1. +9 CONTINUE + CALL GETRL(WMIN,WMAX,DEF,'Position 1',VALUE, + + KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + WT(1) = VALUE + CALL GETRL(WMIN,WMAX,DEF,'Position 2',VALUE, + + KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + WT(2) = VALUE + CALL GETRL(WMIN,WMAX,DEF,'Position 3',VALUE, + + KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + WT(3) = VALUE +C CHECK FOR ZERO + X=WT(1)+WT(2)+WT(3) + IF(X.LT.0.0000001)THEN + WRITE(KBOUT,*) + + 'All weights zero, at least one must be non-zero' + GO TO 9 + END IF + IOK = 0 + END + SUBROUTINE PBD1(SEQ,IDIM1P,MXSPAN,KSTART,IENDB,KBIN,KBOUT, + +SCORES,IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER SEQ(IDIM1P) + REAL SCORES(5,3) + CHARACTER HELPF*(*) + INTEGER CTONUM + EXTERNAL CTONUM +C NOTE KSTART IS THE NUMBER OF THE +C BASE IN ELEMENT 1 OF SEQ, AND IDIM1 IS THE LENGTH FROM KSTART +C STANDARDS CAN +C BE TAKEN FROM KSTART TO KSTART+IDIM1-1 = IENDB + I1INM1=1+(MXSPAN/2)-KSTART+1 + IPASS = 0 +11 CONTINUE + IPASS = IPASS + 1 + K1 = 0 + K2 = 0 + KS1 = KSTART-1 + CALL GTREG(KBIN,KBOUT,KS1,IENDB,K1,K2, + + 'Define region for standard', + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF((K2-K1).LT.191) THEN + CALL ERROM(KBOUT,'At least 64 codons required in standard') + IF(IPASS.LT.2) GO TO 11 + IOK = 1 + RETURN + END IF + DO 1 I=1,5 + DO 1 J=1,3 + SCORES(I,J)=0. +1 CONTINUE + NELTS=0 + DO 2 I=K1,K2-2,3 + NELTS=NELTS+1 + IS=CTONUM(SEQ(I+I1INM1)) + IS1=CTONUM(SEQ(I+I1INM1+1)) + IS2=CTONUM(SEQ(I+I1INM1+2)) + SCORES(IS,1)=SCORES(IS,1)+1. + SCORES(IS1,2)=SCORES(IS1,2)+1. + SCORES(IS2,3)=SCORES(IS2,3)+1. +2 CONTINUE + DO 3 I=1,5 + DO 3 J=1,3 + SCORES(I,J)=SCORES(I,J)/NELTS +3 CONTINUE + IOK = 0 + END + SUBROUTINE GETPBS(SUM,COMP) +C AUTHOR: RODGER STADEN + REAL SUM(4,4,4),COMP(5,3) + TCOMP=0. + DO 50 I=1,5 + DO 50 J=1,3 + COMP(I,J)=0. +50 CONTINUE + DO 55 J=1,4 + DO 55 K=1,4 + DO 55 L=1,4 + TCOMP=TCOMP+SUM(J,K,L) + COMP(J,1)=COMP(J,1)+SUM(J,K,L) + COMP(J,2)=COMP(J,2)+SUM(K,J,L) + COMP(J,3)=COMP(J,3)+SUM(K,L,J) +55 CONTINUE + IF(TCOMP.NE.0) THEN + DO 60 I=1,4 + DO 60 J=1,3 + COMP(I,J)=COMP(I,J)/TCOMP +60 CONTINUE + END IF + END + SUBROUTINE PBASPP(SEQ,IDIM1P,MXSPAN, + +ISXMAX,ISYMAX,J1,J2,KSTART,ANSABS,LENW,IWRIT, + +EXPEC,SCORES,WT,S,SMIN,SMAX, + +MARGL1,MARGR1,MARGB,MARGT,PAA) + CHARACTER PAA(5,5,5) +C AUTHOR: RODGER STADEN + REAL SUMW(3),PROB(3),EXPEC(5,3),SCORES(5,3),WT(3),S(3) + CHARACTER SEQ(IDIM1P) + CHARACTER TLINE(3),STAR + INTEGER ANSABS + SAVE STAR + DATA STAR/'*'/ + CALL FILLR(SUMW,3,0.) + IDONE=0 + IMARG=NINT(MARGT/3.) + MARGB1=MARGB + MARGB2=MARGB+IMARG + MARGB3=MARGB+2*IMARG + MARGT3=IMARG + MARGT2=IMARG + MARGT1=IMARG + I1IN=2+(MXSPAN/2)-KSTART+1 + I1INM1=I1IN-1 + IDIMJ=J2-J1+1. + J1P=J1+I1INM1 + J2P=J2+I1INM1 + IMH=NINT(0.005*ISYMAX) + LB=1+LENW/2 + LB=3*LB + LF=LENW/2 + LF=3*LF + LENW3=3*LENW + YMIN=SMIN + YMAX=SMAX + XMIN=J1 + XMAX=J2 +C set initial values + XP=J1 + YMID=YMIN+(YMAX-YMIN)/2. + YP1=YMID + YP2=YMID + YP3=YMID + CALL CLEARV + CALL VECTOM + XMAX1=XMIN+(XMAX-XMIN)/80. + CALL LINE(XMIN,XMAX1,S(1),S(1),XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL LINE(XMIN,XMAX1,S(2),S(2),XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL LINE(XMIN,XMAX1,S(3),S(3),XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL LINE(XMIN,XMAX1,S(1),S(1),XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + CALL LINE(XMIN,XMAX1,S(2),S(2),XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + CALL LINE(XMIN,XMAX1,S(3),S(3),XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + CALL LINE(XMIN,XMAX1,S(1),S(1),XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) + CALL LINE(XMIN,XMAX1,S(2),S(2),XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) + CALL LINE(XMIN,XMAX1,S(3),S(3),XMAX,XMIN,YMAX,YMIN, + +MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) + CALL FRAME(MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL FRAME(MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + CALL FRAME(MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) + CALL PBASES(SEQ(J1P-LB),IDIM1P,LENW3,SUMW(1),SCORES,WT) + CALL PBASES(SEQ(J1P+1-LB),IDIM1P,LENW3,SUMW(2),SCORES,WT) + CALL PBASES(SEQ(J1P+2-LB),IDIM1P,LENW3,SUMW(3),SCORES,WT) + DO 300 IP=J1P,J2P,3 + IDONE=IDONE+1 + IP0=IP + IP1=IP+1 + IP2=IP+2 + CALL ROTPB1(SEQ,IDIM1P,IP0,LB,LF,SUMW(1),SCORES,WT) + CALL ROTPB1(SEQ,IDIM1P,IP1,LB,LF,SUMW(2),SCORES,WT) + CALL ROTPB1(SEQ,IDIM1P,IP2,LB,LF,SUMW(3),SCORES,WT) + IF(MOD(IDONE,IWRIT).EQ.0)THEN + PROB(1)=SUMW(1) + PROB(2)=SUMW(2) + PROB(3)=SUMW(3) + IF(ANSABS.EQ.0) THEN + T=SUMW(1)+SUMW(2)+SUMW(3) + PROB(1)=PROB(1)/T + PROB(2)=PROB(2)/T + PROB(3)=PROB(3)/T + END IF + CALL FILLC(TLINE,3,' ') + T=MAX(PROB(1),PROB(2),PROB(3)) + DO 303 II=1,3 + IF(PROB(II).EQ.T)TLINE(II)=STAR +303 CONTINUE + IPA=IP-I1INM1 + X=IPA + CALL LINE(XP,X,YP1,PROB(1),XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1+IMH,MARGT1-2*IMH,ISXMAX,ISYMAX) + CALL LINE(XP,X,YP2,PROB(2),XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB2+IMH,MARGT2-2*IMH,ISXMAX,ISYMAX) + CALL LINE(XP,X,YP3,PROB(3),XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB3+IMH,MARGT3-2*IMH,ISXMAX,ISYMAX) + YP1=PROB(1) + YP2=PROB(2) + YP3=PROB(3) + IF(TLINE(1).EQ.STAR)CALL POINT( + + X,YMID,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + IF(TLINE(2).EQ.STAR)CALL POINT( + + X,YMID,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB2,MARGT2,ISXMAX,ISYMAX) + IF(TLINE(3).EQ.STAR)CALL POINT( + + X,YMID,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB3,MARGT3,ISXMAX,ISYMAX) + XP=X + END IF +300 CONTINUE + CALL STARTS(SEQ,IDIM1P,J1P,J2P, + +MARGL1,MARGR1,MARGB,MARGT,ISXMAX,ISYMAX,PAA) + CALL STOPS(SEQ,IDIM1P,J1P,J2P, + +MARGL1,MARGR1,MARGB,MARGT,ISXMAX,ISYMAX,PAA) + CALL VT100M + END + SUBROUTINE PBASES(SEQ,IDIM,LENW,SUM,SCORES,WT) +C AUTHOR: RODGER STADEN + REAL SUM + CHARACTER SEQ(IDIM) + REAL SCORES(5,3),WT(3) + INTEGER CTONUM + EXTERNAL CTONUM + SUM=0. + DO 100 I=1,LENW,3 + SUM=SUM+SCORES(CTONUM(SEQ(I)),1)*WT(1)+ + + SCORES(CTONUM(SEQ(I+1)),2)*WT(2) + + +SCORES(CTONUM(SEQ(I+2)),3)*WT(3) +100 CONTINUE + RETURN + END + SUBROUTINE ROTPB1(SEQ,IDIM1,I,LB,LF,H,SCORES,WT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1) + INTEGER CTONUM + REAL SCORES(5,3),WT(3) + EXTERNAL CTONUM +C set up dummy values + XS=0. + XA=0. +C look back + IPB=I-LB-1 + DO 10 J=1,3 + IF(CTONUM(SEQ(IPB+J)).EQ.5)GO TO 30 +10 CONTINUE +C set value to subtract + IPB=IPB+1 + XS=SCORES(CTONUM(SEQ(IPB)),1)*WT(1)+ + +SCORES(CTONUM(SEQ(IPB+1)),2)*WT(2) + ++SCORES(CTONUM(SEQ(IPB+2)),3)*WT(3) +C now look forward +30 CONTINUE + IPB=I+LF-1 +C look for bad char + DO 45 J=1,3 + IF(CTONUM(SEQ(IPB+J)).EQ.5)GO TO 50 +45 CONTINUE +C ok so use + IPB=IPB+1 + XA=SCORES(CTONUM(SEQ(IPB)),1)*WT(1)+ + +SCORES(CTONUM(SEQ(IPB+1)),2)*WT(2) + ++SCORES(CTONUM(SEQ(IPB+2)),3)*WT(3) +50 H=H-XS+XA + END + SUBROUTINE PBEXP(SCORES,WT,S) +C AUTHOR: RODGER STADEN + REAL SCORES(5,3),S(3),WT(3) + DO 50 I=1,3 + S(I)=0. +50 CONTINUE + DO 60 I=1,4 + S(1)=S(1)+SCORES(I,1)*SCORES(I,1)*WT(1) + + +SCORES(I,2)*SCORES(I,2)*WT(2) + + +SCORES(I,3)*SCORES(I,3)*WT(3) + S(2)=S(2)+SCORES(I,2)*SCORES(I,1)*WT(1) + + +SCORES(I,3)*SCORES(I,2)*WT(2) + + +SCORES(I,1)*SCORES(I,3)*WT(3) + S(3)=S(3)+SCORES(I,3)*SCORES(I,1)*WT(1) + + +SCORES(I,1)*SCORES(I,2)*WT(2) + + +SCORES(I,2)*SCORES(I,3)*WT(3) +60 CONTINUE + END + SUBROUTINE TRNA(SEQ,IDIM,ISS,ISE,KSTART, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + INTEGER CONSC(18) + INTEGER ANSCON,DIALOG + CALL SHOWFU(KBOUT,'tRNA search ') + CALL GETPAR(49,35,IOK, + +MNLEN,MXLEN,MAXLNT, + +MNAC,MXAC,MINACL, + +MNTU,MXTU,MINTU, + +MNAN,MXAN,MINANT, + +MND,MXD,MIND, + +MNI1,MXI1,INT1, + +MNI2,MXI2,JNT2, + +MNTUL1,MXTUL1,LTUMIN, + +MNTUL2,MXTUL2,LTUMAX, + +MNB,MXB,MINBAS, + +MNC,MXC,MINCON,IGON,ANSCON) + IF(IOK.NE.0) RETURN + IF(DIALOG.EQ.1) CALL TRNAD( + +MNLEN,MXLEN,MAXLNT, + +MNAC,MXAC,MINACL, + +MNTU,MXTU,MINTU, + +MNAN,MXAN,MINANT, + +MND,MXD,MIND, + +MNI1,MXI1,INT1, + +MNI2,MXI2,JNT2, + +MNTUL1,MXTUL1,LTUMIN, + +MNTUL2,MXTUL2,LTUMAX, + +MNB,MXB,MINBAS, + +MINCON,IGON,ANSCON, + +CONSC, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL TRNAP(SEQ,IDIM,ISS,ISE,KSTART, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV,KBOUT, + +MAXLNT,MINACL,MINTU,MINANT,MIND,INT1,JNT2, + +LTUMIN,LTUMAX,MINBAS,MINCON,IGON,ANSCON,CONSC) + CALL VT100M + END + SUBROUTINE TRNAP(SEQ,IDIM,ISS,ISE,KSTART, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV,KBOUT, + +MAXLNT,MINACL,MINTU,MINANT,MIND,INT1,JNT2, + +LTUMIN,LTUMAX,MINBAS,MINCON,IGON,ANSCON,CONSC) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + INTEGER RAM,RAN,RTU + INTEGER SCORES(5,5) + INTEGER CONSC(18),TOTCON,CTONUM + INTEGER ANSCON + EXTERNAL CTONUM + SAVE SCORES + DATA SCORES/25*0/ + SCORES(1,3)=2 + SCORES(3,1)=2 + SCORES(1,4)=1 + SCORES(4,1)=1 + SCORES(2,4)=2 + SCORES(4,2)=2 + CALL CLEARV + CALL BUSY(KBOUT) + IF(IGON.EQ.0)THEN + XMIN=ISS + XMAX=ISE +C ymin= half stems base-paired, ymax= all stems base-paired + YMIN=18. + YMAX=42. + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF +C loop for all stem start points + DO 300 N=ISS,ISE + IS=N+60+INT1 + IE=N+MAXLNT+JNT2 + IF(IE.GT.ISE)IE=ISE + IF(IE.LT.IS)RETURN +C STEM LEFT END IS N,RIGHT END RANGES FROM IS TO IE +C LOOP FOR ALL POSITIONS THIS STEM START POINT + DO 200 I=IS,IE +C SET UP POINTER TO LEFT STEM + LAM=N-1 +C SET IANALL TO 0 AS FLAG FOR RE-ENTRY TO ANLOOP + IANALL=0 + ISUM=0 +C LOOP FOR STEM LENGTH 7 + DO 100 J=1,7 +C POINTER TO STEM LEFT END + LAM=LAM+1 +C RIGHT HAND POINTER (MOVES BACKWARDS) + RAM=I-J + ISUM=ISUM+SCORES( + + CTONUM(SEQ(LAM)),CTONUM(SEQ(RAM))) +100 CONTINUE + IF(ISUM.LT.MINACL)GO TO 200 +C SET TULOOP START POINT + MTU=0 +140 CONTINUE + IANALL=0 + CALL TULOOP(SEQ,IDIM,RAM,LTUMIN, + + LTUMAX,MINTU,ISUMTU,SCORES,RTU,LTU,MTU) + IF(ISUMTU.LT.MINTU)GO TO 200 +150 CONTINUE + CALL ANLOOP(SEQ,IDIM,MINANT,ISUMAN,N, + + RTU,SCORES,JNT2,RAN,LAN,IANALL,INT1) + IF(ISUMAN.LT.MINANT)GO TO 140 + CALL DLOOP(SEQ,IDIM,ISUMD,LAM,LAN,SCORES) + IF(ISUMD.GE.MIND)THEN +C HIGH ENOUGH OVERALL SCORE? + ITOTBS=ISUM+ISUMTU+ISUMAN+ISUMD + IF(ITOTBS.GE.MINBAS)THEN +C FILTER ON CONSERVED BASES + IF(ANSCON.EQ.0)THEN + CALL CONSCR(SEQ,IDIM,N, + + LAN,RTU,CONSC,TOTCON) + IF(TOTCON.LT.MINCON) + + GO TO 150 + END IF + ISUMD=ISUMD-MIND + ISUMAN=ISUMAN-MINANT + IF(IGON.EQ.0)THEN + X=N + Y=ITOTBS + CALL LINE(X,X,0.,Y,XMAX,XMIN, + + YMAX,YMIN,MARGL,MARGR,MARGB, + + MARGT,ISXMAX,ISYMAX) + END IF + IF(IGON.EQ.1) CALL TRNADR(SEQ,IDIM,I,N,LAN, + + RAN,LTU,RTU,IDEV,KSTART) +C HAVE ALL POSSIBLE ANTICODONS BEEN TRIED? + END IF + END IF + GO TO 150 +200 CONTINUE +300 CONTINUE + END + SUBROUTINE TRNAD( + +MNLEN,MXLEN,MAXLNT, + +MNAC,MXAC,MINACL, + +MNTU,MXTU,MINTU, + +MNAN,MXAN,MINANT, + +MND,MXD,MIND, + +MNI1,MXI1,INT1, + +MNI2,MXI2,JNT2, + +MNTUL1,MXTUL1,LTUMIN, + +MNTUL2,MXTUL2,LTUMAX, + +MNB,MXB,MINBAS, + +MINCON,IGON,ANSCON, + +CONSC, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + INTEGER CONSC(18),ANSCON + CHARACTER HELPF*(*) + IOK = 1 + CALL GETINT(MNLEN,MXLEN,MAXLNT, + +'Maximum trna length', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXLNT = IVAL + CALL GETINT(MNAC,MXAC,MINACL, + +'Aminoacyl stem score', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINACL = IVAL + CALL GETINT(MNTU,MXTU,MINTU, + +'Tu stem score', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINTU = IVAL + CALL GETINT(MNAN,MXAN,MINANT, + +'Anticodon stem score', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINANT = IVAL + CALL GETINT(MND,MXD,MIND, + +'D stem score', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MIND = IVAL + MNB = MIND + MINTU + MINACL + MINANT +C MXB = MXD + MXTU + MXAC + MNAN + MINBAS = MNB + CALL GETINT(MNB,MXB,MINBAS, + +'Minimum base pairing total', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINBAS = IVAL + CALL GETINT(MNI1,MXI1,INT1, + +'Minimum intron length', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + INT1 = IVAL + MNI2 = INT1 + JNT2 = INT1 +C IF(INT1.GT.0) THEN + CALL GETINT(MNI2,MXI2,JNT2, + + 'Maximum intron length', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + JNT2 = IVAL +C END IF + CALL GETINT(MNTUL1,MXTUL1,LTUMIN, + +'Minimum length for TU loop', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + LTUMIN = IVAL + MNTUL2 = LTUMIN + CALL GETINT(MNTUL2,MXTUL2,LTUMAX, + +'Maximum length for TU loop', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + LTUMAX = IVAL +C conserved bases + CALL YESONO(ANSCON, + +'Search for conserved bases', + +'Skip search for conserved bases', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANSCON.LT.0) THEN + IOK = 1 + RETURN + END IF + IOK = 1 + IF(ANSCON.EQ.0)CALL CONSET(CONSC,MINCON,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(MINCON.LT.0)RETURN + CALL YESONO(IGON,'Plot results','List results', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IGON.LT.0)RETURN + IOK = 0 + END +C +C conset + SUBROUTINE CONSET(CONSC,MINCON,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + INTEGER CONSC(18),NUM(18),CONSER(18) + CHARACTER CONBAS(18) + CHARACTER STRING*35 + SAVE NUM,CONSER,CONBAS + DATA NUM/8,10,11,14,15,21,32,33,37,48,53,54,55,56,57, + +58,60,61/ + DATA CONSER/100,95,96,100,100,97,100,98,91,100,100, + +95,97,100,100,100,92,100/ + DATA CONBAS/'T','G','Y','A','R','A','Y','T','A','Y', + +'G','T','T','C','R','A','Y','C'/ +C SET FLAG FOR RETURN + MINCON=-9 + ITOT = 0 + MN = 0 + MX = 100 + MNT = 0 + WRITE(KBOUT,1002) +1002 FORMAT(' Give a score for each base, then a minimum', + +' total at the end') + DO 10 J=1,18 + STRING = ' ' + WRITE(STRING,1000)NUM(J),CONBAS(J),CONSER(J) + CALL GETINT(MN,MX,MN, + + STRING, + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CONSC(J) = IVAL +1000 FORMAT( 'Base ',I2,', ',A1,' is ',I3,'% conserved.', + + ' Score') + ITOT = ITOT + CONSC(J) +10 CONTINUE + CALL GETINT(MNT,ITOT,MNT, + +'Minimum total conserved base score', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINCON = IVAL + END + SUBROUTINE CONSCR(SEQ,IDIM,N,LAN,RTU,CONSC,TOTCON) +C AUTHOR: RODGER STADEN +C bug fix 6-8-92: previously did not allow for case! + CHARACTER SEQ(IDIM) + INTEGER CONSC(18),NUMS(18),TOTCON,RTU,B,CHAR1(18),CHAR2(18) + INTEGER CTONUM + EXTERNAL CTONUM + SAVE CHAR1,CHAR2,NUMS + DATA CHAR1/1,4,2,3,3,3,2,1,3,2,4,1,1,2,3,3,2,2/ + DATA CHAR2/1,4,1,3,4,3,1,1,3,1,4,1,1,2,4,3,1,2/ + DATA NUMS/7,9,10,13,14,-10,1,2,6,-5,0,1,2,3,4,5,7,8/ +C DATA CHAR1/'T','G','C','A','A','A','C','T','A','C','G', +C + 'T','T','C','A','A','C','C'/ +C DATA CHAR2/'T','G','T','A','G','A','T','T','A','T','G', +C + 'T','T','C','G','A','T','C'/ +C +C test those from left end + TOTCON=0 + DO 10 J=1,5 + K=N+NUMS(J) + B = CTONUM(SEQ(K)) + IF((B.EQ.CHAR1(J)).OR.(B.EQ.CHAR2(J))) + + TOTCON=TOTCON+CONSC(J) +10 CONTINUE +C those from anticodon + DO 20 J=6,9 + K=LAN+NUMS(J) + B = CTONUM(SEQ(K)) + IF((B.EQ.CHAR1(J)).OR.(B.EQ.CHAR2(J))) + + TOTCON=TOTCON+CONSC(J) +20 CONTINUE +C THOSE FROM TU LOOP + DO 30 J=10,18 + K=RTU+NUMS(J) + B = CTONUM(SEQ(K)) + IF((B.EQ.CHAR1(J)).OR.(B.EQ.CHAR2(J))) + + TOTCON=TOTCON+CONSC(J) +30 CONTINUE + END + SUBROUTINE ANLOOP(SEQ,IDIM,MINANT,ISUMAN,N,RTU,SCORES,JNT2, + +RAN,LAN,IANALL,INT1) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + INTEGER SCORES(5,5),RTU,RAN,CTONUM + EXTERNAL CTONUM +C NEED TO TEST FLAG FOR MULTIPLE ENTRY TO THIS SUBROUTINE +C WHEN SEARCHING FOR BEST ANTICODON. IANALL =0 MEANS NO +C POSITIONS TRIED FOR THIS START, IANALL=1 MEANS SOME TRIED +C MIN DIST FROM N TO FIRST BASE IS 6+2+3+4+3+1=19 +C MAX IS TUSTEM-3=RTU-8 +C ALLOWING 3 IN VARIABLE LOOP + IAS=N+19 +C allow 2 in variable loop to be sure + IEND=RTU-7 +C restrict max d loop size to 17 + IAE=N+36 + IF(IAE.GT.IEND)IAE=IEND +C LOOP FOR ALL LEFT STEM STARTS +C NN IS EFFECTIVELY THE LEFT STEM START +C RIGHT STEM RANGE FROM NEAREST TO NEAREST + INTRON +C IE NN+16 TO NN+16+INTRON +C IAS TO IAE DEFINES THE REGION TO THRU TRYING ALL START +C POSITIONS AND ALL INTRON LENGTHS +C TEST FLAG FOR MULTIPLE ENTRY. IF SET START SEARCHING 1 ON FROM +C LAST ANTICODON + IF(IANALL.EQ.1)IAS=LAN-3 +C reset flag + IANALL=1 + ISUMAN=0 + IF(IAE.LT.IAS)RETURN + DO 750 NN=IAS,IAE + IIS=NN+16+INT1 + IIE=NN+16+JNT2 + IF(IIE.GT.IEND)IIE=IEND + IF(IIE.LT.IIS)RETURN +C +C try all positions for the right stem from this left stem start +C + DO 740 I=IIS,IIE +C +C will only try one position if intron=0 +C or intron max=intron min + LAN=NN-1 + ISUMAN=0 + RAN=I+1 +C +C loop for stem +C + DO 730 J=1,5 + LAN=LAN+1 + RAN=RAN-1 + ISUMAN=ISUMAN+SCORES + + (CTONUM(SEQ(RAN)),CTONUM(SEQ(LAN))) +730 CONTINUE +C score high enough? + IF(ISUMAN.GE.MINANT)RETURN +740 CONTINUE +750 CONTINUE + END +C +C dloop + SUBROUTINE DLOOP(SEQ,IDIM,ISUMD,LAM,LAN,SCORES) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + INTEGER SCORES(5,5),RD,CTONUM + EXTERNAL CTONUM +C first base left end is lam+3 +C first base right end is lan-6 +C + LD=LAM+2 + RD=LAN-5 + ISUMD=0 +C + DO 10 I=1,4 + LD=LD+1 + RD=RD-1 + ISUMD=ISUMD+SCORES(CTONUM(SEQ(LD)),CTONUM(SEQ(RD))) +10 CONTINUE + END +C trnadr + SUBROUTINE TRNADR(SEQ,IDIM,I,N,LAN,RAN,LTU,RTU,IDEV,KSTART) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + CHARACTER BLANK + CHARACTER ARRAY(30,30) + INTEGER RAN,RTU + CHARACTER SCORES(5,5),SCOREZ(5,5) + INTEGER CTONUM + EXTERNAL CTONUM + SAVE SCORES,SCOREZ,BLANK + DATA BLANK/' '/ + DATA SCORES/25*' '/,SCOREZ/25*' '/ + SCORES(1,3)='-' + SCORES(3,1)='-' + SCORES(2,4)='-' + SCORES(4,2)='-' + SCORES(1,4)='+' + SCORES(4,1)='+' + SCOREZ(1,3)='!' + SCOREZ(3,1)='!' + SCOREZ(2,4)='!' + SCOREZ(4,2)='!' + SCOREZ(1,4)='+' + SCOREZ(4,1)='+' +C +C blank arrays + DO 10 J=1,30 + DO 10 K=1,30 + ARRAY(K,J)=BLANK +10 CONTINUE +C aminoacyl + ARRAY(16,5)=(SEQ(I)) + J=I-1 + K=N + DO 20 L=6,12 + ARRAY(14,L)=(SEQ(K)) + ARRAY(16,L)=(SEQ(J)) + ARRAY(15,L)=SCORES(CTONUM(SEQ(K)),CTONUM(SEQ(J))) + K=K+1 + J=J-1 +20 CONTINUE +C +C gap between aminoacyl d stem + ARRAY(13,13)=(SEQ(N+7)) + ARRAY(12,14)=(SEQ(N+8)) +C +C +C anticodon + K=LAN-4 + J=RAN+4 + DO 30 L=19,23 + ARRAY(13,L)=(SEQ(K)) + ARRAY(15,L)=(SEQ(J)) + ARRAY(14,L)=SCORES(CTONUM(SEQ(K)),CTONUM(SEQ(J))) + K=K+1 + J=J-1 +30 CONTINUE +C +C anticodon loop + ARRAY(12,24)=(SEQ(LAN+1)) + ARRAY(12,25)=(SEQ(LAN+2)) + ARRAY(16,25)=(SEQ(LAN+6)) + ARRAY(16,24)=(SEQ(RAN-1)) + K=LAN+3 + DO 35 L=13,15 + ARRAY(L,26)=(SEQ(K)) + K=K+1 +35 CONTINUE +C +C gap between dstem and anticodon + ARRAY(12,18)=(SEQ(LAN-5)) +C +C tustem + K=LTU+4 + L=RTU-4 + DO 40 J=17,21 + ARRAY(J,13)=(SEQ(K)) + ARRAY(J,15)=(SEQ(L)) + ARRAY(J,14)=SCOREZ(CTONUM(SEQ(K)),CTONUM(SEQ(L))) + K=K-1 + L=L+1 +40 CONTINUE +C tu loop +C length of loop + LNGTH=K-L+1 + IF(LNGTH.GT.18)RETURN +C return if loop length lt 3 + IF(LNGTH.LT.3)RETURN +C number in outer + LU=(LNGTH-1)/2 + M=22 + DO 45 J=1,LU + ARRAY(M,12)=(SEQ(K)) + ARRAY(M,16)=(SEQ(L)) + K=K-1 + L=L+1 + M=M+1 +45 CONTINUE +C + ARRAY(M,15)=(SEQ(L)) + L=L+1 + IF(MOD(LNGTH,2).EQ.0)ARRAY(M,14)=(SEQ(L)) +C +C +46 CONTINUE +C dstem + K=N+9 + L=LAN-6 + LNGTH=L-K+1 + J2=4 + M=11 + IF(LNGTH.GT.10)GO TO 47 +C need stem of 3 not 4 + M=10 + J2=3 +47 CONTINUE + DO 50 J=1,J2 + ARRAY(M,15)=(SEQ(K)) + ARRAY(M,17)=(SEQ(L)) + ARRAY(M,16)=SCOREZ(CTONUM(SEQ(K)),CTONUM(SEQ(L))) + K=K+1 + L=L-1 + M=M-1 +50 CONTINUE +C +C dloop +C length of loop + LNGTH=L-K+1 + IF(LNGTH.GT.14)RETURN +C if loop length lt 3 return + IF(LNGTH.LT.3)RETURN +C number in outer + LU=(LNGTH-1)/2 + DO 80 J=1,LU + ARRAY(M,14)=(SEQ(K)) + ARRAY(M,18)=(SEQ(L)) + K=K+1 + L=L-1 + M=M-1 +80 CONTINUE + ARRAY(M,17)=(SEQ(L)) + L=L-1 + IF(MOD(LNGTH,2).EQ.0)ARRAY(M,16)=(SEQ(L)) +C +C +85 CONTINUE +C variable loop + LNGTH=(RTU-5)-(RAN+5)+1 + IF(LNGTH.GT.26)RETURN + IF(LNGTH.LT.3)RETURN +C number of elements in lower diagonal + LL=(LNGTH/2)-1 +C correction made in june 81 for lngthg=3 + IF(LL.EQ.0)LL=1 +C lsi must go thru loops even with 0! +C number in upper + LU=(LNGTH+1)/2 +C do lower + K=16 + L=19 + M=RAN+5 + DO 60 J=1,LL + ARRAY(K,L)=(SEQ(M)) + K=K+1 + L=L+1 + M=M+1 +60 CONTINUE +C check for silly loop size + IF(LNGTH.LT.3)RETURN +C put in extra base + K=K+1 + L=L-1 + IF(LNGTH.GT.3)ARRAY(K,L)=(SEQ(M)) +C do upper + K=17 + L=16 + M=RTU-5 + DO 70 J=1,LU + ARRAY(K,L)=(SEQ(M)) + K=K+1 + L=L+1 + M=M-1 +70 CONTINUE +C +C output + WRITE(IDEV,1002)N+KSTART-1 +1002 FORMAT(/5X,I6) + DO 200 M=1,30 + DO 100 J=1,30 + IF(ARRAY(J,M).EQ.BLANK)GO TO 100 + WRITE(IDEV,1000)(ARRAY(L,M),L=1,30) + GO TO 200 +100 CONTINUE +200 CONTINUE +1000 FORMAT(5X,30A) + END + SUBROUTINE TULOOP(SEQ,IDIM,RAM,LTUMIN,LTUMAX,MINTU,ISUMTU, + +SCORES,RTU,LTU,MTU) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + INTEGER SCORES(5,5) + INTEGER RAM,RTU,RAMM2,CTONUM + EXTERNAL CTONUM + ISUMTU=0 +C loop must start at ram-1 +C vary loop from ltumin to ltumax + RAMM2=RAM-2 + M=LTUMIN+9 + N=LTUMAX+9 +C is this first pass thru this aminoacyl? + IF(MTU.GT.0)M=MTU+1 +C all done? + IF(M.GT.N)RETURN +C + DO 650 I=M,N + ISUMTU=0 + RTU=RAMM2-I + DO 640 J=1,5 + LTU=RAM-J + RTU=RTU+1 + ISUMTU=ISUMTU+SCORES( + + CTONUM(SEQ(RTU)),CTONUM(SEQ(LTU))) +640 CONTINUE + MTU=I +C score high enough? + IF(ISUMTU.GE.MINTU)RETURN +650 CONTINUE + END + SUBROUTINE CODMPP(SEQ,IDIM,FTABLE,IDFTAB, + +KBOUT,J1,J2,I1,I2,IDIM1,IDEV) +C AUTHOR: RODGER STADEN + REAL RNFAC,FTABLE(IDFTAB),FACTOR,FACN + INTEGER NBASE(4),NCOD(4,4,4) + EXTERNAL FACTOR + CHARACTER SEQ(IDIM) + INTEGER NCODON(21),IACID(4,4,4) + REAL FABC(4,4,4),FA(21) +C array with number of codons per acid + SAVE IACID,NCODON + DATA IACID/ + +1,2,3,5,6,7,8,9, + +10,12,14,16,18,20,6,21, + +1,2,3,5,6,7,8,9, + +10,12,14,16,18,20,6,21, + +2,2,3,5,6,7,8,9, + +11,13,15,17,11,20,20,21, + +2,2,4,5,6,7,8,9, + +11,13,15,17,19,20,20,21/ + DATA NCODON/ + +2,6,3,1,4,6,4,4,4,2,3,2,2,2,2,2,2,2,1,6,4/ + LB=150 + LF=147 + IF(IDIM1.GT.310)THEN + CALL BUSY(KBOUT) + FACN=FACTOR(99,FTABLE,IDFTAB) + SUMY=0. +C do calc + CALL ROT2(SEQ(I1-J1+1),IDIM1,NCOD,RNFAC,FTABLE,IDFTAB, + + NBASE,297,FA,FABC,NCODON,IACID) + COMP=0. + DO 90 J=I1+LB-J1+1,I2-LF-2-J1+1,3 + CALL ROTFAK(SEQ,IDIM,J,LB,LF, + + NCOD,RNFAC,FTABLE,IDFTAB, + + NBASE,COMP,297,99., + + FA,FABC,NCODON,IACID) + W=RNFAC-COMP-FACN + CALL POISON(99,FABC,FABC,EW,EWD,2) + Y=(W-EW)/EWD + SUMY=SUMY+Y +90 CONTINUE + SUMY=SUMY/((I2-LF-2-(I1+LB))/3.) + WRITE(IDEV,5555)SUMY +5555 FORMAT(' Mean (W-EW)/EWD, window 99 ',F10.1) + END IF + END + SUBROUTINE PREPT1(SEQ,POSNS,IDIM,J1IN,J2IN,KSTART, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IDEV,DIALOG, + +WORDP,WORDN,MAXWRD,MAXDIC) + INTEGER WORDP(MAXDIC),WORDN(MAXDIC) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + INTEGER POSNS(IDIM),DIALOG + CHARACTER SEQ(IDIM) + INTEGER R + CALL SHOWFU(KBOUT,'Plot repeats') + CALL GETPAR(32,4,IOK,MINRP,MAXRP,MINREP,IGON, + +IPAR5, + +IPAR6,IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + L = J1IN + R = J2IN + IF(DIALOG.EQ.1) THEN + CALL YESONO(IGON,'Plot results','List results', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IGON.LT.0)RETURN + CALL GTREG(KBIN,KBOUT,J1IN,J2IN,L,R, + +'Define restricted region', + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL GETINT(MINRP,MAXRP,MINREP, + +'Minimum repeat', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINREP = IVAL + END IF + IDIM1=R-L+1 + IDIM2=J2IN-J1IN+1 + L1=L-J1IN+1 + CALL BUSY(KBOUT) + CALL REPEAT(SEQ(L-KSTART+1), + +POSNS,IDIM1,MINREP,L1,J1IN,IDIM2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV,IGON, + +WORDP,WORDN,MAXWRD,MAXDIC) + CALL VT100M + END +C REPEAT + SUBROUTINE REPEAT(SEQ,POSNS,IDIM,MINR,L,KSTART,IDIM1, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV,IGON, + +WORDP,WORDN,MAXWRD,MAXDIC) + INTEGER WORDP(MAXDIC),WORDN(MAXDIC) +C AUTHOR: RODGER STADEN + INTEGER POSNS(IDIM),CONST(6) + CHARACTER SEQ(IDIM) + LENGTH=MAXWRD + LE4=MAXDIC + CALL ENCO(SEQ,IDIM,POSNS,CONST,LENGTH) + CALL ENCONA(POSNS,IDIM,WORDP,WORDN,LE4,LENGTH) + CALL REPT1(SEQ,IDIM,IDIM1,L,KSTART, + +POSNS,WORDN,WORDP,LENGTH,LE4,MINR, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV,IGON) + END + SUBROUTINE REPT1(SEQ,IDIM,IDIM1,LEFT,KSTART, + +POSNS,WORDN,WORDP,LENGTH,LE4,MINR, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV,IGON) +C AUTHOR: RODGER STADEN + INTEGER POSNS(IDIM),WORDP(LE4) + INTEGER WORDN(LE4) + CHARACTER SEQ(IDIM) +C POSNS CONTAIN THE WORD NUMBERS ENCODED BY 4**LENGTH +C WORDN CONTAINS THE NUMBER OF OCCURENCES OF EACH POSSIBLE WORD +C WORDP CONTAINS THE POINTERS TO THE FIRST OCCURRENCE OF THE WORDS +C ENCODED--- EG POINTER TO FIRST OCCURRENCE OF THE WORD ENCODED AS 101 +C WILL BE STORED IN ELEMENT 101 + IF(IGON.EQ.0) CALL VECTOM + XMIN=1. + XMAX=IDIM1 + YMIN=0. + YMAX=1. + X1=LEFT + Y1=0. + YINC=1./IDIM1 +C LOOK FOR MATCHES BY THOSE ELEMENTS OF WORDN>1 + DO 100 I=1,LE4 + IF(WORDN(I).GT.1)THEN +C POINT TO FIRST POSITION AS ITS IN WORDP + IP=WORDP(I) +C A MATCH, TRY ALL PAIRS + DO 50 J=1,WORDN(I) +C COMPARE THIS POSITION WITH ALL OTHERS + IPS=IP + DO 40 K=J+1,WORDN(I) +C FIND LENGTH OF MATCH + L=LENGTH + IP1=IP+LENGTH + IPS=POSNS(IPS) + IP2=IPS+LENGTH +20 CONTINUE + IF((IP1.LE.IDIM).AND.(IP2.LE.IDIM))THEN + IF(SEQ(IP1).EQ.SEQ(IP2))THEN + IP1=IP1+1 + IP2=IP2+1 + L=L+1 + GO TO 20 + END IF + END IF +C END OF MATCH, IS IT LONG ENOUGH? + IF(L.GE.MINR)THEN + X1=LEFT+IP-1 + X2=LEFT+IPS-1 + Y2=Y1+YINC*ABS(X2-X1) + IF(IGON.EQ.0)THEN + CALL LINE(X1,X1,Y1,Y2, + + XMAX,XMIN,YMAX,YMIN,MARGL,MARGR, + + MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(X1,X2,Y2,Y2, + + XMAX,XMIN,YMAX,YMIN,MARGL,MARGR, + + MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(X2,X2,Y2,Y1, + + XMAX,XMIN,YMAX,YMIN,MARGL,MARGR, + + MARGB,MARGT,ISXMAX,ISYMAX) + END IF + IF(IGON.EQ.1)THEN + INTX1=X1+KSTART-1 + INTX2=X2+KSTART-1 + WRITE(IDEV,1000)INTX1,INTX2,L,(SEQ(KK),KK=IP,IP+L-1) + END IF + END IF +1000 FORMAT(' ',I6,2X,I6,2X,I6,(' ',50A1)) +40 CONTINUE + IP=POSNS(IP) +50 CONTINUE + END IF +100 CONTINUE + END + SUBROUTINE PREPT2(SEQ,POSNS,IDIM,J1IN,J2IN,KSTART, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IDEV,DIALOG, + +WORDP,WORDN,MAXWRD,MAXDIC) + INTEGER WORDP(MAXDIC),WORDN(MAXDIC) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + INTEGER POSNS(IDIM),DIALOG + CHARACTER SEQ(IDIM) + INTEGER R + CALL SHOWFU(KBOUT,'Plot long-range inverted repeats') + CALL GETPAR(31,4,IOK,MINRP,MAXRP,MINREP,IGON, + +IPAR5, + +IPAR6,IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + L = J1IN + R = J2IN + IF(DIALOG.EQ.1) THEN + CALL YESONO(IGON,'Plot results','List results', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IGON.LT.0)RETURN + CALL GTREG(KBIN,KBOUT,J1IN,J2IN,L,R, + +'Define restricted region', + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL GETINT(MINRP,MAXRP,MINREP, + +'Minimum inverted repeat', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINREP = IVAL + END IF + IDIM1=R-L+1 + IDIM2=J2IN-J1IN+1 + L1=L-J1IN+1 + CALL BUSY(KBOUT) + CALL REPT2(SEQ(L-KSTART+1),POSNS,IDIM1,MINREP,L1,J1IN,IDIM2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV,IGON, + +WORDP,WORDN,MAXWRD,MAXDIC) + CALL VT100M + END +C REPT2 + SUBROUTINE REPT2(SEQ,POSNS,IDIM,MINR,L,KSTART,IDIM1, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV,IGON, + +WORDP,WORDN,MAXWRD,MAXDIC) + INTEGER WORDP(MAXDIC),WORDN(MAXDIC) +C AUTHOR: RODGER STADEN + INTEGER POSNS(IDIM),CONST(6) + CHARACTER SEQ(IDIM) + LENGTH=MAXWRD + LE4=MAXDIC + CALL ENCO(SEQ,IDIM,POSNS,CONST,LENGTH) + CALL ENCONA(POSNS,IDIM,WORDP,WORDN,LE4,LENGTH) + CALL REPT3(SEQ,IDIM,IDIM1,L,KSTART, + +POSNS,WORDN,WORDP,LENGTH,LE4,MINR, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV,IGON) + END + SUBROUTINE REPT3(SEQ,IDIM,IDIM1,LEFT,KSTART, + +POSNS,WORDN,WORDP,LENGTH,LE4,MINR, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV,IGON) +C AUTHOR: RODGER STADEN + INTEGER POSNS(IDIM),WORDP(LE4) + INTEGER WORDN(LE4),CONST(6) + CHARACTER SEQ(IDIM),SCOMP + EXTERNAL SCOMP +C POSNS CONTAIN THE WORD NUMBERS ENCODED BY 4**LENGTH +C WORDN CONTAINS THE NUMBER OF OCCURENCES OF EACH POSSIBLE WORD +C WORDP CONTAINS THE POINTERS TO THE FIRST OCCURRENCE OF THE WORDS +C ENCODED--- EG POINTER TO FIRST OCCURRENCE OF THE WORD ENCODED AS 101 +C WILL BE STORED IN ELEMENT 101 + IF(IGON.EQ.0) CALL VECTOM + XMIN=1. + XMAX=IDIM1 + YMIN=0. + YMAX=1. + X1=LEFT + Y1=0. + YINC=1./IDIM1 +C SET UP CONST FOR ROUTINE THAT LOOKS FOR COMPLEMENTARY WORD POINTERS + ISTART=1 + DO 1 I=1,LENGTH + CONST(I)=4**(I-1) + ISTART=ISTART-CONST(I) +1 CONTINUE +C +C LOOK FOR MATCHES BY THOSE ELEMENTS OF WORDN>1 + DO 100 I=1,LE4 + IF(WORDN(I).GE.1)THEN +C POINT TO FIRST POSITION AS ITS IN WORDP + IP=WORDP(I) +C DOES ITS COMPLEMENT EXIST? + JCOMPN=ICOMPN(SEQ,IDIM,IP,CONST,LENGTH,ISTART) + NCOMP=WORDN(JCOMPN) + IF(NCOMP.NE.0)THEN +C YES, NEED TO COMPARE ALL PAIRS IE COMPARE WORDN(I) POSITIONS +C WITH NCOMP POSITIONS. POINT TO FIRST OCCURRENCE + IPN=WORDP(JCOMPN) +C A MATCH, TRY ALL PAIRS + DO 50 J=1,WORDN(I) +C COMPARE THIS POSITION WITH ALL OTHERS + IPN1=IPN + DO 40 K=1,NCOMP +C FIND LENGTH OF MATCH + L=LENGTH + IP1=IP+LENGTH + IP2=IPN1-1 +20 CONTINUE + IF((IP1.LE.IDIM).AND.(IP2.GT.1))THEN + IF(SEQ(IP1).EQ.SCOMP(SEQ(IP2)))THEN + IP1=IP1+1 + IP2=IP2-1 + L=L+1 + GO TO 20 + END IF + END IF + IF(L.GE.MINR)THEN + X1=LEFT+IP-1 + X2 = LEFT + IP2 +C X2=LEFT+IPN1-3 + Y2=Y1+YINC*ABS(X2-X1) + IF(IGON.EQ.0)THEN + CALL LINE(X1,X1,Y1,Y2, + + XMAX,XMIN,YMAX,YMIN,MARGL,MARGR, + + MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(X1,X2,Y2,Y2, + + XMAX,XMIN,YMAX,YMIN,MARGL,MARGR, + + MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(X2,X2,Y2,Y1, + + XMAX,XMIN,YMAX,YMIN,MARGL,MARGR, + + MARGB,MARGT,ISXMAX,ISYMAX) + END IF + IF(IGON.EQ.1)THEN + INTX1=X1+KSTART-1 + INTX2=X2+KSTART-1 + IF(IP.EQ.IPN1) INTX2 = INTX1 + WRITE(IDEV,1000)INTX1,INTX2,L,(SEQ(KK),KK=IP,IP+L-1) + END IF + END IF + IPN1=POSNS(IPN1) +1000 FORMAT(' ',I6,2X,I6,2X,I6,(' ',50A1)) +40 CONTINUE +C POINT TO NEXT WORD + IP=POSNS(IP) +50 CONTINUE + WORDN(JCOMPN)=0 + END IF +C SET THIS WORD TO ZERO SO WE DONT USE IT AGAIN + WORDN(I)=0 + END IF +100 CONTINUE + END + INTEGER FUNCTION ICOMPN(SEQ,IDIM,IP,CONST,LENGTH,ISTART) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + INTEGER CONST(LENGTH),CTONUM + EXTERNAL CTONUM,ICOMP + N=ISTART + L=IP+LENGTH + K=0 + DO 1 J=1,LENGTH + K=K+1 + L=L-1 + N=N+CONST(K)*ICOMP(CTONUM(SEQ(L))) +1 CONTINUE + ICOMPN=N + END + SUBROUTINE DISIG(SEQ,IDIM,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,IDEV,FILNAM, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IDEVOT, + +SUM,WT,TOT,IDM,MAXLEN,LINE,CHRSET,DIALOG) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),FILNAM*(*),HELPF*(*) + CHARACTER LINE(MAXLEN),CHRSET(IDM) + INTEGER SUM(IDM,IDM,MAXLEN),TOT(MAXLEN),DIALOG + REAL WT(IDM,IDM,MAXLEN) + CALL SHOWFU(KBOUT, + +'Motif search using dinucleotide weight matrix') + CALL GETPAR(60,1,IOK,IGON, + +IPAR2,IPAR3,IPAR4,IPAR5, + +IPAR6,IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + CALL DISIGD(IDEV,FILNAM, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH, + +SUM,IDM,MAXLEN,TOT,WT,LENGTH,MIDDLE,YMIN,YMAX, + +IGON,IDEVOT,LINE,CHRSET,DIALOG,IOK) + IF(IOK.NE.0) RETURN + CALL DISIGP(SEQ,IDIM,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,IDEVOT,WT,IDM,MAXLEN,LENGTH, + +YMIN,YMAX,IGON,MIDDLE) + END + SUBROUTINE DISIGD(IDEV,FILNAM, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH, + +SUM,IDM,MAXLEN,TOT,WT,LENGTH,MIDDLE,YMIN,YMAX, + +IGON,IDEVOT,LINE,CHRSET,DIALOG,IOK) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),HELPF*(*),LINE(MAXLEN),CHRSET(IDM) + INTEGER SUM(IDM,IDM,MAXLEN),TOT(MAXLEN),DIALOG + REAL WT(IDM,IDM,MAXLEN) + PARAMETER (MAXPRM = 21) + CHARACTER PROMPT(3)*(MAXPRM) + IOK = 1 + IDO = 1 + PROMPT(1) = 'Use weight matrix' + PROMPT(2) = 'Make weight matrix' + PROMPT(3) = 'Rescale weight matrix' + CALL RADION('Select operation',PROMPT,3,IDO, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IDO.LT.1) RETURN + IF(IDO.GT.1) THEN + CALL MKWTD(WT,SUM,TOT,CHRSET,IDM,MAXLEN, + + IDEV,IDEVOT,KBIN,KBOUT,LINE, + + FILNAM,IDO,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IOK = 1 + RETURN + END IF + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + +'Motif weight matrix file', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + LENGTH=MAXLEN + CALL RDWMTD(TOT,SUM,MIDDLE,LENGTH,MAXLEN,YMIN,YMAX,IDEV, + + IOK,IDM,KBOUT) + IF(IOK.NE.0)THEN + CALL ERROM(KBOUT,'Error in weight matrix') + RETURN + END IF + IF(DIALOG.EQ.1) THEN + AMN = -9999. + AMX = 9999. + CALL GETRL(AMN,AMX,YMIN,'Cutoff score',VAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + YMIN = VAL + END IF + IF(YMIN.LT.0.0)CALL GETWD(TOT,SUM,WT,LENGTH,IDM,MAXLEN) + IF(YMIN.GE.0.0)CALL GETW2D(SUM,WT,LENGTH,IDM,MAXLEN) + CALL YESONO(IGON,'Plot results','List results', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IGON.LT.0)RETURN + IOK = 0 + END + SUBROUTINE GETCD(TOT,SUM,LINE,IDM,MAXLEN,IDEV,KBOUT,LENGTH, + +IOK) + INTEGER TOT(MAXLEN),SUM(IDM,IDM,MAXLEN) + CHARACTER LINE(MAXLEN) + INTEGER CTONUM + EXTERNAL CTONUM + IOK = 1 + DO 2 I=1,MAXLEN + TOT(I)=0 + DO 1 J=1,IDM + DO 1 K=1,IDM + SUM(K,J,I)=0 +1 CONTINUE +2 CONTINUE + N=0 +10 CONTINUE +1003 FORMAT(1X,120A1) +1004 FORMAT(' ',I6,' ',120A1) + READ(IDEV,1003,END=100)LINE + N=N+1 + WRITE(KBOUT,1004)N,LINE + DO 20 I=1,MAXLEN + IF(LINE(I).EQ.' ')GO TO 10 + J = CTONUM(LINE(I+1)) + K = CTONUM(LINE(I)) + SUM(K,J,I) = SUM(K,J,I) + 1 +20 CONTINUE + GO TO 10 +100 CONTINUE + IF(N.EQ.0)THEN + CALL ERROM(KBOUT,'Empty file of aligned sequences') + RETURN + END IF +C NOW FIND LENGTH OF MOTIF + DO 40 I=1,MAXLEN + K=0 + L=I + DO 30 J=1,IDM + DO 30 M=1,IDM + K=K+SUM(M,J,I) +30 CONTINUE + IF(K.EQ.0)GO TO 50 + TOT(I)=TOT(I)+K +40 CONTINUE +50 CONTINUE + LENGTH=L-2 + IOK = 0 + END + SUBROUTINE GETWD(TOT,SUM,FREQ,LENGTH,MAXCHR,MAXLEN) + INTEGER TOT(LENGTH),SUM(MAXCHR,MAXCHR,MAXLEN) + REAL FREQ(MAXCHR,MAXCHR,MAXLEN) + DO 70 I=1,LENGTH + DO 60 J=1,MAXCHR + DO 60 K=1,MAXCHR + FREQ(K,J,I)=LOG((REAL(SUM(K,J,I)+1)/REAL(TOT(I)+MAXCHR))) +60 CONTINUE +70 CONTINUE + END + SUBROUTINE GETW2D(SUM,FREQ,LENGTH,MAXCHR,MAXLEN) + INTEGER SUM(MAXCHR,MAXCHR,MAXLEN) + REAL FREQ(MAXCHR,MAXCHR,MAXLEN) + DO 70 I=1,LENGTH + DO 60 J=1,MAXCHR + DO 60 K=1,MAXCHR + FREQ(K,J,I)=REAL(SUM(K,J,I)) +60 CONTINUE +70 CONTINUE + END + SUBROUTINE MKWTD(FREQ,SUM,TOT,CHRSET,IDM,MAXLEN, + +IDEV2,IDEV3,KBIN,KBOUT,LINE, + +FILNAM,IOPT,IHELPS,IHELPE,HELPF,IDEVH,IOK) +C AUTHOR RODGER STADEN + CHARACTER FILNAM*(*),HELPF*(*) + CHARACTER LINE(MAXLEN),TITLE*60,CHRSET(IDM) + INTEGER SUM(IDM,IDM,MAXLEN),TOT(MAXLEN) + REAL FREQ(IDM,IDM,MAXLEN) + IOK = 1 + IF(IOPT.EQ.3)THEN + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,0,IOK,KBIN,KBOUT, + + 'Name of existing weight matrix file', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL RDWMTD(TOT,SUM,MIDDLE,LENGTH,MAXLEN, + + CUTMIN,CUTMAX,IDEV2, + + IOK,IDM,KBOUT) + IF(IOK.NE.0) RETURN + END IF + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,0,IOK,KBIN,KBOUT, + +'Name of aligned sequences file', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + IF(IOPT.EQ.2) THEN + CALL GETCD(TOT,SUM,LINE,IDM,MAXLEN,IDEV2, + + KBOUT,LENGTH,IOK) + IF(IOK.NE.0)RETURN + END IF + WRITE(KBOUT,1006)LENGTH +1006 FORMAT(' Length of motif',I6) + IOK = 1 + CALL YESNO(IOPT,'Sum logs of weights', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOPT.LT.0) RETURN + CUTMIN = -10.0 + IF(IOPT.EQ.1)CUTMIN = 10. + CALL MASKWD(SUM,LENGTH,IDM,MAXLEN,KBIN,KBOUT,TITLE, + +IOPT,IHELPS,IHELPE,HELPF,IDEVH) + IF(IOPT.LT.0) RETURN +C NOW CALC WEIGHTS + IF(CUTMIN.LT.0.0)CALL GETWD(TOT,SUM,FREQ,LENGTH,IDM,MAXLEN) + IF(CUTMIN.GE.0.0)CALL GETW2D(SUM,FREQ,LENGTH,IDM,MAXLEN) +C NOW APPLY THE WEIGHTS + REWIND IDEV2 + CALL APPLWD(FREQ,IDM,LENGTH,IDEV2,IDEV3,KBIN,KBOUT,LINE,MAXLEN, + +BOT,TOP,TITLE,MIDDLE,IOK) + CLOSE(UNIT=IDEV2) + IF(IOK.NE.0) RETURN + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,1,IOK,KBIN,KBOUT, + +'Name for new weight matrix file', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL WRTSCD(TITLE,LENGTH,MIDDLE,BOT,TOP,IDM, + +TOT,SUM,CHRSET,IDEV2,MAXLEN) + CLOSE(UNIT=IDEV2) + RETURN + END + SUBROUTINE MASKWD(SUM,LENGTH,IDM,MAXLEN,KBIN,KBOUT,MASK, + +IOPT,IHELPS,IHELPE,HELPF,IDEVH) + INTEGER SUM(IDM,IDM,MAXLEN) + CHARACTER MASK*(*),HELPF*(*) + CALL YESNO(IOPT,'Use all motif positions', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOPT.LT.0) RETURN +5 CONTINUE + IF(IOPT.EQ.1)THEN + WRITE(KBOUT,1002) +1002 FORMAT(' x means use, - means ignore',/, + + ' e.g. xx-x---x-x means use positions 1,2,4,8,10') + LIN = 0 + CALL GTSTR('Mask',' ',MASK,LIN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 5 + END IF + IF(INFLAG.EQ.2) RETURN + IF(LIN.EQ.0) RETURN + DO 70 I=1,LENGTH + IF(MASK(I:I).EQ.'-')THEN + DO 60 J=1,IDM + DO 60 K=1,IDM + SUM(K,J,I) = 0 +60 CONTINUE + END IF +70 CONTINUE + END IF + END + SUBROUTINE APPLWD(FREQ,IDM,LENGTH,IDEV,IDEV3,KBIN,KBOUT, + +LINE,MAXLEN,BOT,TOP,TITLE,MIDDLE,IOK) + REAL FREQ(IDM,IDM,MAXLEN) + CHARACTER LINE(MAXLEN),TITLE*(*) + INTEGER CTONUM + EXTERNAL CTONUM + IOK = 1 + N=0 + TOP=-99999. + BOT=9999999. + WRITE(KBOUT,*)' Applying weights to input sequences' + SMEAN = 0. + SUMSQ = 0. +1003 FORMAT(1X,120A1) +1004 FORMAT(' ',I4,' ',F12.3,' ',120A1) +200 CONTINUE + READ(IDEV,1003,END=300)LINE + N=N+1 + SCORE=0. + DO 210 I=1,LENGTH + J = CTONUM(LINE(I+1)) + K = CTONUM(LINE(I)) + SCORE=SCORE+FREQ(K,J,I) +210 CONTINUE + WRITE(IDEV3,1004)N,SCORE,(LINE(K),K=1,LENGTH) + IF(SCORE.GT.TOP)TOP=SCORE + IF(SCORE.LT.BOT)BOT=SCORE + SMEAN=SMEAN+SCORE + SUMSQ=SUMSQ+SCORE*SCORE + GO TO 200 +300 CONTINUE + IF(N.LT.1)THEN + CALL ERROM(KBOUT,'Error: empty sequence file') + RETURN + END IF + SMEAN=SMEAN/N + SM=SMEAN + SMEAN=SMEAN*SMEAN + SUMSQ=SUMSQ/N + SD = 0. + T = SUMSQ - SMEAN + IF(T.GT.0.)SD = SQRT(T) + SMM3=SM-3*SD + SMP3=SM+3*SD + WRITE(KBOUT,1000)TOP,BOT +1000 FORMAT(' Top score',F12.3,' Bottom score',F12.3) + WRITE(KBOUT,1001)SM,SD +1001 FORMAT(' Mean',F12.3,' Standard deviation',F12.3) + WRITE(KBOUT,1002)SMM3,SMP3 +1002 FORMAT(' Mean minus 3.sd',F12.3,' Mean plus 3.sd',F12.3) + BOT=SMM3 + TOP=SMP3 + XMN = -999. + XMX = 9999. + CALL GETRL(XMN,XMX,BOT,'Cutoff score',VAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + BOT = VAL + XMN = BOT + XMX = 999. + CALL GETRL(XMN,XMX,TOP,'Top score for scaling plots', + +VAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + TOP = VAL + MN = 0 + MX = LENGTH + MIDDLE = 1 + CALL GETINT(MN,MX,MIDDLE,'Position to identify',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MIDDLE = IVAL +305 CONTINUE + LIN = 0 + CALL GTSTR('Title',' ',TITLE,LIN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 305 + END IF + IF(INFLAG.EQ.2) RETURN + IOK = 0 + END + SUBROUTINE GETW1D(SUM,FREQ,LENGTH,MAXCHR,MAXLEN) + INTEGER SUM(MAXCHR,MAXCHR,MAXLEN) + REAL FREQ(MAXCHR,MAXCHR,MAXLEN) + DO 70 I=1,LENGTH + DO 60 J=1,MAXCHR + DO 60 K=1,MAXCHR + FREQ(K,J,I) = 0. + IF(SUM(K,J,I).GT.0)FREQ(K,J,I) = 1. +60 CONTINUE +70 CONTINUE + END + SUBROUTINE WRTSCD(TITLE,LENGTH,MIDDLE,BOT,TOP,IDM, + +TOT,SUM,CHRSET,IDEV,MAXLEN) + INTEGER TOT(LENGTH),SUM(IDM,IDM,MAXLEN) + CHARACTER CHRSET(IDM),TITLE*(*) +C PROTEIN MATRICES DONT WRITE ROWS FOR -X? AND SPACE SO SET DIMENSION +C TO IDM-4 + MINUS = 1 + IF(IDM.EQ.26)MINUS = 4 + WRITE(IDEV,1018)TITLE +1018 FORMAT(' ',A) +1019 FORMAT(' P',20I4) +1020 FORMAT(' N',20I4) +1021 FORMAT(' ',A,A,20I4) +1022 FORMAT(' ',2I6,2F10.3) + WRITE(IDEV,1022)LENGTH,MIDDLE,BOT,TOP + NLINES=1+(LENGTH-1)/20 + K1=1 + DO 400 J=1,NLINES + K2=MIN((K1+19),LENGTH) + WRITE(IDEV,1019)(K,K=K1-MIDDLE,K2-MIDDLE) + WRITE(IDEV,1020)(TOT(K),K=K1,K2) + DO 390 I=1,IDM-MINUS + DO 390 K=1,IDM-MINUS + WRITE(IDEV,1021)CHRSET(K),CHRSET(I),(SUM(K,I,KK),KK=K1,K2) +390 CONTINUE + K1=K1+20 + IF(K1.GT.LENGTH)K1=LENGTH +400 CONTINUE + CLOSE(UNIT=IDEV) + END + SUBROUTINE RDWMTD(TOT,WT,MIDDLE,LENGTH,MAXLEN,YMIN,YMAX,IDEV, + +IFAIL,IDM,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER WT(5,5,MAXLEN),TOT(MAXLEN) + CHARACTER LINE*79 +C SET FAIL FLAG + IFAIL=1 +1000 FORMAT( ) +1001 FORMAT(3X,20I4) +1002 FORMAT(1X,2I6,2F10.3) +1003 FORMAT(A) +1004 FORMAT(' ',A) + DO 3 I = 1,MAXLEN + TOT(I) = 0 + DO 2 J = 1,5 + DO 1 K = 1,5 + WT(K,J,I) = 0 +1 CONTINUE +2 CONTINUE +3 CONTINUE +C READ TITLE + READ(IDEV,1003,ERR=100,END=100)LINE + WRITE(KBOUT,1004)LINE +C READ PLOT VALUES ETC + READ(IDEV,1002,ERR=100,END=100) + +LENGTH,MIDDLE,YMIN,YMAX +C HOW MANY LINES TO READ? + NLINES=1+(LENGTH-1)/20 + K1=1 + DO 10 JJ=1,NLINES + READ(IDEV,1000,ERR=100,END=100) + K2=MIN((K1+19),LENGTH) +C READ(IDEV,1001,ERR=100,END=100)(TOT(K),K=K1,K2) + READ(IDEV,1000,ERR=100,END=100) + DO 5 I=1,4 + DO 5 J=1,4 + READ(IDEV,1001,ERR=100,END=100)(WT(J,I,K),K=K1,K2) + DO 6 K=K1,K2 + TOT(K) = TOT(K) + WT(J,I,K) +6 CONTINUE +5 CONTINUE + K1=K1+20 + IF(K1.GT.LENGTH)K1=LENGTH +10 CONTINUE + CLOSE(UNIT=IDEV) +C SET FAIL FLAG TO GOOD + IFAIL=0 + RETURN +100 CONTINUE + END + SUBROUTINE DISIGP(SEQ,IDIM,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,IDEVOT,WT,IDM,MAXLEN,LENGTH, + +YMIN,YMAX,IGON,MIDDLE) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + REAL WT(IDM,IDM,MAXLEN) + INTEGER CTONUM + EXTERNAL CTONUM + XMAX=J2 + XMIN=J1 + IF(IGON.EQ.0)THEN + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + DO 10 I=J1,J2-LENGTH + SUM1=0. + K=0 + DO 5 J=I,I+LENGTH-1 + K=K+1 + KT1=CTONUM(SEQ(J)) + KT2=CTONUM(SEQ(J+1)) + SUM1 = SUM1 + WT(KT1,KT2,K) +5 CONTINUE + IF(SUM1.GE.YMIN)THEN + IF(IGON.EQ.0)THEN + X=I+MIDDLE + CALL LINE(X,X,YMIN,SUM1,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + IF(IGON.EQ.1)THEN + CALL VT100M + WRITE(IDEVOT,1010)I+MIDDLE,SUM1,(SEQ(K),K=I,I+LENGTH-1) +1010 FORMAT(' ',I7,F10.2,' ',120A1) + END IF + END IF +10 CONTINUE + CALL VT100M + RETURN + END +C LOCALF + SUBROUTINE LOCALF(SEQNCE,IDIM1,STRING,MATCH,IDIM3I, + +ITOT,ITOTEL,ITOTID, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,J1,J2,KSTART, + +IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQNCE(IDIM1),STRING(IDIM3I),MATCH(IDIM3I) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + INTEGER ANSRV,ANSC + INTEGER SPAN,DIALOG + CALL SHOWFU(KBOUT, + +'Search for local similarity or complementarity') + CALL GETPAR(36,15,IOK,MINSP,MAXSP,SPAN,MINPR,MAXPR,IPR, + +MNIR1,MXIR1,IRAN1,MNIR2,MXIR2,IRAN2, + +ANSRV,ANSC,IGON, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + PR = REAL(IPR) +10 CONTINUE + CALL LOCALD( + +ANSRV,ANSC,IOK,MINSP,MAXSP,SPAN,MINPR,MAXPR,IPR,PR, + +MNIR1,MXIR1,IRAN1,MNIR2,MXIR2,IRAN2,IGON, + +J1,J2,IS,IE,KBIN,KBOUT,MINS, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL LOCALP(SEQNCE,IDIM1,STRING,MATCH, + +ITOT,ITOTEL,ITOTID, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,KSTART, + +ANSRV,SPAN,IRAN1,IRAN2,IGON,MINS, + +J1,J2,IS,IE,KBIN,KBOUT,IDEV) + DIALOG = 1 + GO TO 10 + END + SUBROUTINE LOCALD( + +ANSRV,ANSC,IOK,MINSP,MAXSP,SPAN,MINPR,MAXPR,IPR,PR, + +MNIR1,MXIR1,IRAN1,MNIR2,MXIR2,IRAN2,IGON, + +J1,J2,IS,IE,KBIN,KBOUT,MINS, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + INTEGER ANSRV,ANSC + INTEGER CHOICE,SPAN + IOK = 1 + CHOICE = ANSRV + CALL YESONO(CHOICE,'Find direct repeats', + +'Find inverted repeats', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(CHOICE.LT.0) RETURN + ANSRV = CHOICE + CALL YESNO(ANSC,'Keep picture', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANSC.LT.0) RETURN + IF(ANSC.EQ.1)CALL CLEARG + CALL GETINT(MINSP,MAXSP,SPAN,'Span',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + SPAN = IVAL + IS = J1 + IE = J2 + CALL GTREG(KBIN,KBOUT,0,J2,IS,IE,'Define restricted region', + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL SQPF5(SPAN,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PR,MINS,IOK) + IF(IOK.NE.0) RETURN + CALL GETINT(MNIR1,MXIR1,IRAN1,'Range start',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IRAN1 = IVAL + MNIR2 = MAX(MNIR2,IRAN1) + MXIR2 = MAX(MNIR2,MXIR2) + IRAN2 = MAX(IRAN2,MNIR2) + CALL GETINT(MNIR2,MXIR2,IRAN2,'Range end',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IRAN2 = IVAL + CALL YESONO(IGON,'Plot results','List results', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IGON.LT.0)RETURN + IOK = 0 + END + SUBROUTINE LOCALP(SEQNCE,IDIM1,STRING,MATCH, + +ITOT,ITOTEL,ITOTID, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,KSTART, + +ANSRV,SPAN,IRAN1,IRAN2,IGON,MINS, + +J1,J2,IS,IE,KBIN,KBOUT,IDEV) +C AUTHOR: RODGER STADEN + INTEGER SPAN + CHARACTER SEQNCE(IDIM1),STRING(SPAN),MATCH(SPAN) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + INTEGER ANSRV + XMIN=J1 + XMAX=J2 + YMIN=0. + YMAX=SPAN + CALL BUSY(KBOUT) + IF(IGON.EQ.0)THEN + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF +C LAST START POSITION + ILAST=IE-SPAN+1 +C HOW MANY LOOPS? + IT1=IRAN1+SPAN-1 + IT2=IRAN2+SPAN+SPAN-2 + DO 50 J=IS,IE + IS1=J+IT1 + IE1=J+IT2 + IF(IE1.GT.IE)IE1=IE + IF(IS1.GT.ILAST)GO TO 51 + CALL SQCOPY(SEQNCE(J-KSTART+1),STRING,SPAN) + IF(ANSRV.EQ.1)THEN + CALL SQREV(STRING,SPAN) + CALL SQCOM(STRING,SPAN) + END IF + CALL SQFIT(SEQNCE,IDIM1,STRING,SPAN,ITOT,ITOTEL,ITOTID, + + IS1,IE1,MINS,ITOTP,KSTART) + IF(ITOTP.GT.ITOTID) THEN + IF(IGON.EQ.0)CALL VT100M + WRITE(KBOUT,1017)ITOTID +1017 FORMAT(/10X,'More than',I4,' matches.', + + ' Try changing percentage or region'/) + RETURN + END IF + IF(ITOTP.GT.0)THEN + IF(IGON.EQ.0)THEN + X1=J + DO 41 I=1,ITOTP + X=ITOTEL(I) + Y=ITOT(I) + CALL LINE(X1,X,Y,YMIN, + + XMAX,XMIN,YMAX,YMIN,MARGL,MARGR,MARGB, + + MARGT,ISXMAX,ISYMAX) +41 CONTINUE + END IF + IF(IGON.EQ.1)THEN + DO 49 I=1,ITOTP + K=ITOTEL(I)-KSTART+1 + WRITE(IDEV,1008) +1008 FORMAT(/) + CALL SQMTCH(SEQNCE(K),STRING,MATCH,SPAN) + CALL FMT4LN(SEQNCE(K),STRING,MATCH, + + SPAN,ITOTEL(I),J,IDEV) +49 CONTINUE + END IF + END IF +50 CONTINUE +51 CONTINUE + IF(IGON.EQ.0)CALL VT100M + END +C SETGEN + SUBROUTINE SETGEN(PAAS,PAA,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER PAAS(5,5,5),PAA(5,5,5) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*),UGA(3),AUA(3),CUA(3),AGA(3),AGG(3) + INTEGER CTONUM + PARAMETER (MAXPRM = 23) + CHARACTER PROMPT(4)*(MAXPRM) + EXTERNAL CTONUM + SAVE UGA,AUA,CUA,AGA,AGG + DATA UGA,AUA,CUA,AGA,AGG/'T','G','A','A','T','A','C','T','A', + +'A','G','A','A','G','G'/ + NUM = 1 +C SET STANDARD CODE + DO 10 I=1,5 + DO 10 J=1,5 + DO 10 K=1,5 + PAA(I,J,K)=PAAS(I,J,K) +10 CONTINUE + PROMPT(1) = 'Standard' + PROMPT(2) = 'Mammalian mitochondrial' + PROMPT(3) = 'Yeast mitochondrial' + PROMPT(4) = 'Personal' + CALL RADION('Select genetic code',PROMPT,4,NUM, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(NUM.LT.2) RETURN + IF(NUM.EQ.2)THEN + PAA(CTONUM(AUA(3)),CTONUM(AUA(2)),CTONUM(AUA(1)))='M' + PAA(CTONUM(UGA(3)),CTONUM(UGA(2)),CTONUM(UGA(1)))='W' + PAA(CTONUM(AGA(3)),CTONUM(AGA(2)),CTONUM(AGA(1)))='*' + PAA(CTONUM(AGG(3)),CTONUM(AGG(2)),CTONUM(AGG(1)))='*' + ELSE IF(NUM.EQ.3)THEN + PAA(CTONUM(CUA(3)),CTONUM(CUA(2)),CTONUM(CUA(1)))='T' + PAA(CTONUM(AUA(3)),CTONUM(AUA(2)),CTONUM(AUA(1)))='M' + PAA(CTONUM(UGA(3)),CTONUM(UGA(2)),CTONUM(UGA(1)))='W' + ELSE IF(NUM.EQ.4)THEN + CALL GETCOD(PAA,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) + END IF + END + SUBROUTINE GETCOD(PAA,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + CHARACTER PAA(5,5,5),AA,CODON*3,AIN,AAA + INTEGER CTONUM + EXTERNAL CTONUM + WRITE(KBOUT,1004) +1004 FORMAT(' Define genetic code by typing a codon', + +/,' followed by a 1 letter amino acid symbol') +1 CONTINUE + L = 0 + CALL GTSTR('Codon',' ',CODON,L,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.3) RETURN + AA = + +PAA(CTONUM(CODON(3:3)),CTONUM(CODON(2:2)),CTONUM(CODON(1:1))) + IF(AA.NE.'-')THEN +2 CONTINUE + L = 1 + AAA = AA + CALL GTSTR('Amino acid symbol',AAA,AIN,L,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 2 + END IF + IF(INFLAG.EQ.2) RETURN + IF(L.GT.0) AAA = AIN + PAA(CTONUM(CODON(3:3)),CTONUM(CODON(2:2)), + + CTONUM(CODON(1:1))) = AAA + GO TO 1 + END IF + GO TO 1 + END + SUBROUTINE DINUCF(SEQ,IDIM,J1,J2,IDEV,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),BASE(4) + REAL OBS(5,5),EXPEC(5,5),COMP(5) + INTEGER CTONUM + EXTERNAL CTONUM + SAVE BASE + DATA BASE/'T','C','A','G'/ + CALL SHOWFU(KBOUT,'Calculate dinucleotide frequencies') + DO 10 I=1,5 + COMP(I)=0. + DO 10 J=1,5 + EXPEC(I,J)=0. + OBS(I,J)=0. +10 CONTINUE + DO 20 I=J1,J2-1 + K0=CTONUM(SEQ(I)) + K1=CTONUM(SEQ(I+1)) + COMP(K0)=COMP(K0)+1. + OBS(K0,K1)=OBS(K0,K1)+1. +20 CONTINUE + TOT=J2-J1 + DO 25 I=1,5 + COMP(I)=COMP(I)/TOT +25 CONTINUE + TOT=TOT/100. + DO 26 I=1,5 + DO 26 J=1,5 + EXPEC(I,J)=COMP(I)*COMP(J) +26 CONTINUE + DO 30 I=1,5 + DO 30 J=1,5 + EXPEC(I,J)=EXPEC(I,J)*100. + OBS(I,J)=OBS(I,J)/TOT +30 CONTINUE + WRITE(IDEV,1002)BASE + WRITE(IDEV,1001) +1002 FORMAT(/10X,4(4X,A1,9X)) +1001 FORMAT(' ',7X,4(' Obs Expected')) + DO 40 K=1,4 + WRITE(IDEV,1000)BASE(K),(OBS(K,J),EXPEC(K,J),J=1,4) +1000 FORMAT(' ',A1,8F7.2) +40 CONTINUE + END + INTEGER FUNCTION ICOMP(IN) +C AUTHOR: RODGER STADEN + INTEGER COMPS(5) + SAVE COMPS + DATA COMPS/3,4,1,2,5/ + ICOMP=COMPS(IN) + END +C lwrap2 + INTEGER FUNCTION LWRAP2(IDIM,I) +C AUTHOR: RODGER STADEN + LWRAP2=I + IF(LWRAP2.LT.1)LWRAP2=LWRAP2+IDIM+1 + IF(LWRAP2.GT.IDIM)LWRAP2=LWRAP2-IDIM + END +C LWRAP3 + INTEGER FUNCTION LWRAP3(IDIM,I) +C AUTHOR: RODGER STADEN + LWRAP3=I + IF(LWRAP3.LT.1)LWRAP3=LWRAP3+IDIM + IF(LWRAP3.GT.IDIM)LWRAP3=LWRAP3-IDIM + END + SUBROUTINE NORMAA(SUM,TOTA,PAA) + CHARACTER PAA(5,5,5),AA(21) + REAL SUM(4,4,4) + SAVE AA + DATA AA/'A','C','D','E','F', + +'G','H','I','K','L', + +'M','N','P','Q','R', + +'S','T','V','W','Y','*'/ +C LOOP FOR EACH ACID + DO 150 L=1,21 + ASUM=0. +C LOOP FOR EACH CODON + DO 200 I=1,4 + DO 200 J=1,4 + DO 200 K=1,4 +C COUNT CODONS + IF(PAA(K,J,I).EQ.AA(L))ASUM=ASUM+SUM(I,J,K) +200 CONTINUE + IF(ASUM.NE.0.0)ASUM=TOTA/ASUM +C LOOP FOR EACH CODON + DO 100 I=1,4 + DO 100 J=1,4 + DO 100 K=1,4 +C NORMALIZE + IF(PAA(K,J,I).EQ.AA(L))SUM(I,J,K)=SUM(I,J,K)*ASUM +100 CONTINUE +150 CONTINUE + END + SUBROUTINE PPROM1(SEQ,IDIM,ISXMAX,ISYMAX,I1,I2,MARGL,MARGR, + + MARGB,MARGT,IDEV,FILEIN,KBOUT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),FILEIN*(*) + INTEGER CTONUM + REAL WTM35(25,5),WTM10(19,5),WTP1(12,5),PENALT(7) + EXTERNAL CTONUM + CALL SHOWFU(KBOUT,'E coli promoter search') + CALL RPPROM(WTM35,WTM10,WTP1,IDEV,FILEIN,IFAIL) + IF(IFAIL.NE.0)THEN + CALL ERROM(KBOUT,'Error in weight matrix') + RETURN + END IF + IMARG=NINT(MARGT/2.0) + MARGB2=MARGB+IMARG + MARGT2=IMARG + MARGB1=MARGB + MARGT1=IMARG + PENALT(1)=0.02 + PENALT(2)=0.2 + PENALT(3)=1.0 + PENALT(4)=0.2 + PENALT(5)=0.05 + PENALT(6)=0.02 + PENALT(7)=0.01 + DO 1 I=1,7 + PENALT(I)=LOG(PENALT(I)) +1 CONTINUE + CUTM35=-36.85 + CUTM10=-28.13 + CUTP1=-21.8 + XMAX=I2 + XMIN=I1 + YMIN=-77.3 + YMAX=-60.4 + YMIN1=-77.3*0.01 + YMAX1=-60.4*0.01 + YMIN1=-77.3 + YMAX1=-60.4 + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL FRAME(MARGL,MARGR,MARGB2,MARGT2,ISXMAX,ISYMAX) + DO 100 I=I1,I2-65 +C DO -35 REGION + SUMM35=0. + K=0 + DO 5 J=I,I+24 + K=K+1 + SUMM35=SUMM35+WTM35(K,CTONUM(SEQ(J))) +5 CONTINUE +C HIGH ENOUGH? + IF(SUMM35.GE.CUTM35)THEN +C YES, TRY -10 REGION + BESTM1=-99999. + DO 20 J=I+25,I+25+6 + K=0 + SUMM10=0. + DO 10 L=J,J+18 + K=K+1 + SUMM10=SUMM10 + WTM10(K,CTONUM(SEQ(L))) +10 CONTINUE +C IS THIS -10 SCORE HIGH ENOUGH? + IF((SUMM10).GE.CUTM10)THEN +C YES, IS IT THE HIGHEST? + IF(SUMM10.GT.BESTM1)THEN + BESTM1=SUMM10 + IBEST=J + IBESTA=IBEST-I-24 + END IF + END IF +20 CONTINUE +C -10 DONE, HIGH ENOUGH SCORE? + IF(BESTM1.GE.CUTM10)THEN +C YES, DO +1 REGION + BESTP1=-99999. + DO 40 J=IBEST+19,IBEST+19+4 + SUMP1=0. + K=0 + DO 30 L=J,J+11 + K=K+1 + SUMP1=SUMP1 + WTP1(K,CTONUM(SEQ(L))) +30 CONTINUE +C HIGH ENOUGH? + IF(SUMP1.GE.CUTP1)THEN +C YES, HIGHEST? + IF(SUMP1.GT.BESTP1)THEN + BESTP1=SUMP1 + JBEST=J + END IF + END IF +40 CONTINUE +C +1 REGION DONE, HIGH ENOUGH SCORE? + IF(BESTP1.GE.CUTP1)THEN +C YES, SO ALL HIGH ENOUGH + X=JBEST+2 + Y=SUMM35+BESTM1+BESTP1 + CALL LINE(X,X,YMIN,Y,XMAX,XMIN, + + YMAX,YMIN, + + MARGL,MARGR,MARGB2,MARGT2, + + ISXMAX,ISYMAX) + Y=Y+PENALT(IBESTA) + CALL LINE(X,X,YMIN1,Y,XMAX,XMIN, + + YMAX1,YMIN1, + + MARGL,MARGR,MARGB1,MARGT1, + + ISXMAX,ISYMAX) + END IF + END IF + END IF +100 CONTINUE + CALL VT100M + END + SUBROUTINE RPPROM(WTM35,WTM10,WTP1,IDEV,FILEIN,IFAIL) +C AUTHOR: RODGER STADEN + REAL WTM35(25,5),WTM10(19,5),WTP1(12,5) + INTEGER WT(25),TOT(25) + CHARACTER FILEIN*(*) + IFAIL=0 +1001 FORMAT(2X,25I3) + CALL OPENRS(IDEV,FILEIN,IOK,LRECL,2) + IF(IOK.NE.0)GO TO 100 +C READ SPACE,P + READ(IDEV,1000,ERR=100) + READ(IDEV,1000,ERR=100) + READ(IDEV,1000,ERR=100) +1000 FORMAT() + READ(IDEV,1001,ERR=100)TOT + DO 5 I=1,4 + READ(IDEV,1001,ERR=100)(WT(K),K=1,25) + DO 5 J=1,25 + IF(WT(J).NE.0)THEN + WTM35(J,I) = LOG(FLOAT(WT(J))/FLOAT(TOT(J))) + ELSE + WTM35(J,I)=LOG(0.5/FLOAT(TOT(J))) + END IF +5 CONTINUE +C DO 5'S + DO 20 I=1,25 + WTM35(I,5)=LOG(0.25/TOT(I)) +20 CONTINUE +C do -10 region + READ(IDEV,1000,ERR=100) + READ(IDEV,1000,ERR=100) + READ(IDEV,1001)(TOT(K),K=1,19) + DO 30 I=1,4 + READ(IDEV,1001,ERR=100)(WT(K),K=1,19) + DO 30 J=1,19 + IF(WT(J).NE.0)THEN + WTM10(J,I) = LOG(FLOAT(WT(J))/FLOAT(TOT(J))) + ELSE + WTM10(J,I)=LOG(0.5/FLOAT(TOT(J))) + END IF +30 CONTINUE +C do 5's + DO 35 I=1,19 + WTM10(I,5)=LOG(0.25/TOT(I)) +35 CONTINUE +C DO +1 REGION + READ(IDEV,1000,ERR=100) + READ(IDEV,1000,ERR=100) + READ(IDEV,1001,ERR=100)(TOT(K),K=1,12) + DO 40 I=1,4 + READ(IDEV,1001,ERR=100)(WT(K),K=1,12) + DO 40 J=1,12 + IF(WT(J).NE.0)THEN + WTP1(J,I) = LOG(FLOAT(WT(J))/FLOAT(TOT(J))) + ELSE + WTP1(J,I)=LOG(0.5/FLOAT(TOT(J))) + END IF +40 CONTINUE +C DO 5'S + DO 45 I=1,12 + WTP1(I,5)=LOG(0.25/TOT(I)) +45 CONTINUE + CLOSE(UNIT=IDEV) + RETURN +100 CONTINUE + IFAIL=1 + END + SUBROUTINE PPROM2(SEQ,IDIM,ISXMAX,ISYMAX,I1,I2,MARGL,MARGR, + +MARGB,MARGT,IDEV,FILEIN,KBOUT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),FILEIN*(*) + REAL WTM35(25,5),WTM10(19,5),WTP1(12,5) + INTEGER CTONUM + EXTERNAL CTONUM + CALL SHOWFU(KBOUT,'E coli promoter -35 and -10 regions search') + CALL RPPROM(WTM35,WTM10,WTP1,IDEV,FILEIN,IFAIL) + IF(IFAIL.NE.0)THEN + CALL ERROM(KBOUT,'Error in weight matrix') + RETURN + END IF + IMARG=NINT(MARGT/2.0) + MARGB2=MARGB+IMARG + MARGT2=IMARG + MARGB1=MARGB + MARGT1=IMARG + XMAX=I2 + XMIN=I1 + YMIN35=-38.0 + YMAX35=-23.5 + YMIN1=-29.0 + YMAX1=-18.4 + YMID35=YMIN35+(YMAX35-YMIN35)/2. + YMID1=YMIN1+(YMAX1-YMIN1)/2. + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL FRAME(MARGL,MARGR,MARGB2,MARGT2,ISXMAX,ISYMAX) + CALL TEXT(XMIN,YMID35,'-35',3,0,XMAX,XMIN,YMAX35,YMIN35, + +MARGL,MARGR,MARGB1,MARGT1,ISXMAX,ISYMAX) + CALL TEXT(XMIN,YMID1,'-10',3,0,XMAX,XMIN,YMAX1,YMIN1, + +MARGL,MARGR,MARGB2,MARGT2,ISXMAX,ISYMAX) +C + DO 100 I=I1,I2-25 +C DO -35 REGION + SUMM35=0. + K=0 + DO 5 J=I,I+24 + K=K+1 + SUMM35=SUMM35+WTM35(K,CTONUM(SEQ(J))) +5 CONTINUE + X=I+48 + Y=SUMM35 + CALL LINE(X,X,YMIN35,Y,XMAX,XMIN,YMAX35,YMIN35, + + MARGL,MARGR,MARGB2,MARGT2,ISXMAX,ISYMAX) +100 CONTINUE + DO 200 I=I1,I2-19 + SUMM10=0. + K=0 + DO 20 J=I,I+18 + K=K+1 + SUMM10=SUMM10+WTM10(K,CTONUM(SEQ(J))) +20 CONTINUE + X=I+22 + Y=SUMM10 + CALL LINE(X,X,YMIN1,Y,XMAX,XMIN,YMAX1,YMIN1, + + MARGL,MARGR,MARGB1,MARGT1,ISXMAX,ISYMAX) +200 CONTINUE + CALL VT100M + END +C PROMOTERS ON THE COMPLEMENTARY STRAND + SUBROUTINE PPROM3(SEQ,IDIM,ISXMAX,ISYMAX,I1,I2,MARGL1,MARGR1, + +MARGB1,MARGT1,IDEV,FILEIN,KBOUT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),FILEIN*(*) + REAL WTM35(25,5),WTM10(19,5),WTP1(12,5) + REAL WTM35C(25,5),WTM10C(19,5),WTP1C(12,5) + INTEGER CTONUM + EXTERNAL CTONUM + CALL SHOWFU(KBOUT,'E coli promters on the complementary strand') + CALL RPPROM(WTM35,WTM10,WTP1,IDEV,FILEIN,IFAIL) + IF(IFAIL.NE.0)THEN + CALL ERROM(KBOUT,'Error in weight matrix') + RETURN + END IF +C REVERSE AND COMPLEMENT MATRICES + DO 5 I=1,25 + DO 5 J=1,2 + WTM35C(I,J+2)=WTM35(26-I,J) + WTM35C(I,J)=WTM35(26-I,J+2) +5 CONTINUE + DO 6 I=1,19 + DO 6 J=1,2 + WTM10C(I,J+2)=WTM10(20-I,J) + WTM10C(I,J)=WTM10(20-I,J+2) +6 CONTINUE + DO 7 I=1,12 + DO 7 J=1,2 + WTP1C(I,J+2)=WTP1(13-I,J) + WTP1C(I,J)=WTP1(13-I,J+2) +7 CONTINUE + DO 1 I = 1,25 + WTM35C(I,5)=WTM35(26-I,5) +1 CONTINUE + DO 2 I = 1,19 + WTM10C(I,5)=WTM10(20-I,5) +2 CONTINUE + DO 3 I = 1,12 + WTP1C(I,5)=WTP1(13-I,5) +3 CONTINUE + CUTM35=-36.85 + CUTM10=-28.13 + CUTP1=-21.8 + XMAX=I2 + XMIN=I1 + YMIN=-77.3 + YMAX=-60.4 + CALL CLEARV + CALL VECTOM + CALL FRAME(MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX) + DO 100 I=I1,I2-65 +C DO +1 REGION + SUMP1=0. + K=0 + DO 8 J=I,I+11 + K=K+1 + SUMP1=SUMP1+WTP1C(K,CTONUM(SEQ(J))) +8 CONTINUE +C HIGH ENOUGH? + IF(SUMP1.GE.CUTP1)THEN +C YES, TRY -10 REGION + BESTM1=-99999. + DO 20 J=I+12,I+12+4 + K=0 + SUMM10=0. + DO 10 L=J,J+18 + K=K+1 + SUMM10=SUMM10 + WTM10C(K,CTONUM(SEQ(L))) +10 CONTINUE +C IS THIS -10 SCORE HIGH ENOUGH? + IF(SUMM10.GE.CUTM10)THEN +C YES, IS IT THE HIGHEST? + IF(SUMM10.GT.BESTM1)THEN + BESTM1=SUMM10 + IBEST=J + END IF + END IF +20 CONTINUE +C -10 DONE, HIGH ENOUGH SCORE? + IF(BESTM1.GE.CUTM10)THEN +C YES, DO -35 REGION + BESTM3=-99999. + DO 40 J=IBEST+19,IBEST+19+6 + SUMM35=0. + K=0 + DO 30 L=J,J+24 + K=K+1 + SUMM35=SUMM35+WTM35C(K,CTONUM(SEQ(L))) +30 CONTINUE +C HIGH ENOUGH? + IF(SUMM35.GE.CUTM35)THEN +C YES, HIGHEST? + IF(SUMM35.GT.BESTM3)THEN + BESTM3=SUMM35 + END IF + END IF +40 CONTINUE +C -35 REGION DONE, HIGH ENOUGH SCORE? + IF(BESTM3.GE.CUTM35)THEN +C YES, SO ALL HIGH ENOUGH + X=I+10 + Y=SUMP1+BESTM3+BESTM1 + CALL LINE(X,X,YMIN,Y, + + XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1, + + MARGT1,ISXMAX,ISYMAX) + END IF + END IF + END IF +100 CONTINUE + CALL VT100M + END + SUBROUTINE PRIBS(SEQ,IDIM,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,I1,I2,IDEV,FILE1,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),FILE1*(*) + INTEGER WEIGHT(505),MARGB1(3),MARGT1(3),CTONUM + EXTERNAL CTONUM + CALL SHOWFU(KBOUT,'Search for E coli ribosome binding sites') +C SORT OUT MARGIN IE FIND MAX AND DIVIDE BY THREE + IMARG=NINT(MARGT/3.) + MARGL1=MARGL + MARGR1=MARGR + MARGB1(1)=MARGB + MARGB1(2)=MARGB+IMARG + MARGB1(3)=MARGB+2*IMARG + MARGT1(1)=IMARG + MARGT1(2)=IMARG + MARGT1(3)=IMARG + YMAX=100. + YMIN=0. + CALL RPRIBS(IDEV,FILE1,WEIGHT,IOK,KBOUT) + IF(IOK.NE.0)RETURN + MINSMN = -50 + MINSMX = 100 + MINSI = 2 + CALL GETINT(MINSMN,MINSMX,MINSI,'Minimum score', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINS = IVAL + XMAX=I2 + XMIN=I1 + Y=0. + YP=20.0 + CALL CLEARV + CALL BUSY(KBOUT) + CALL VECTOM + DO 10 I=I1,I2-100 + ISUM=0 + K=0 + II=I + III=I+100 + DO 5 J=II,III + K=K+1 + KK=CTONUM(SEQ(J))-1 + KK=KK*101 + IP=K+KK + ISUM=ISUM+WEIGHT(IP) +5 CONTINUE + IF(ISUM.GT.MINS)THEN + X=I+60 + IMARG=1+MOD((II-I1),3) + CALL LINE(X,X,Y,YP,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB1(IMARG),MARGT1(IMARG), + + ISXMAX,ISYMAX) + END IF +10 CONTINUE + CALL VT100M + END + SUBROUTINE RPRIBS(IDEV,FILE1,WT,IOK,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER WT(505) + CHARACTER FILE1*(*) +1001 FORMAT(2X,25I3) + CALL OPENRS(IDEV,FILE1,IOK,LRECL,2) + IF(IOK.NE.0)GO TO 100 + DO 20 J=1,4 +C read space,p + READ(IDEV,1000,END=200,ERR=200) + READ(IDEV,1000,END=200,ERR=200) +1000 FORMAT() + JJ=(J-1)*25 + DO 5 I=1,4 + II=(I-1)*101 + K1=JJ+II+1 + READ(IDEV,1001,END=200,ERR=200)(WT(K),K=K1,K1+24) +5 CONTINUE +20 CONTINUE +C do last bit + READ(IDEV,1000,END=200,ERR=200) + READ(IDEV,1000,END=200,ERR=200) + K1=101 +1002 FORMAT(2X,I3) + DO 30 I=1,4 + READ(IDEV,1002,ERR=200,END=200)WT(K1) + K1=K1+101 +30 CONTINUE + CLOSE(UNIT=IDEV) + DO 40 I=405,505 + WT(I)=0 +40 CONTINUE + RETURN +100 CONTINUE + CALL ERROM(KBOUT,'Error opening file') + RETURN +200 CONTINUE + CALL ERROM(KBOUT,'Error reading file') + IOK = 1 + END +C SCANS FOR EUKARYOTIC RIBOSOME BINDING SITES + SUBROUTINE RIBEUK(SEQ,IDIM,ISXMAX,ISYMAX,J1,J2, + +MARGL,MARGR,MARGB,MARGT,IDEV,FILEIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),FILEIN*(*) + REAL WT(10,5) + INTEGER CTONUM,MARGB1(3),MARGT1(3) + EXTERNAL CTONUM + CALL SHOWFU(KBOUT,'Search for eukaryotic ribosome binding sites') + CALL RRIBEU(WT,IDEV,FILEIN,IFAIL) + IF(IFAIL.NE.0)THEN + CALL ERROM(KBOUT,'Error in weight matrix') + RETURN + END IF +C SORT OUT MARGIN IE FIND MAX AND DIVIDE BY THREE + IMARG=NINT(MARGT/3.) + MARGL1=MARGL + MARGR1=MARGR + MARGB1(1)=MARGB + MARGB1(2)=MARGB+IMARG + MARGB1(3)=MARGB+2*IMARG + MARGT1(1)=IMARG + MARGT1(2)=IMARG + MARGT1(3)=IMARG + YMIN=-9.0 + YMAX=0.0 + XMAX=J2 + XMIN=J1 + CALL CLEARV + CALL VECTOM +C ADD 2 TO MAKE PHASE CORRECT + I1=J1+1 + DO 20 II=1,3 + DO 10 I=I1+II,J2-10,3 + IF((SEQ(I+7).EQ.'A').AND.(SEQ(I+8).EQ.'T'). + + AND.(SEQ(I+9).EQ.'G'))THEN + SUM=0. + K=0 + DO 5 J=I,I+9 + K=K+1 + SUM=SUM+WT(K,CTONUM(SEQ(J))) +5 CONTINUE + X=I+7 + CALL LINE(X,X,YMIN,SUM,XMAX, + + XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1(II), + + MARGT1(II),ISXMAX,ISYMAX) + END IF +10 CONTINUE +20 CONTINUE + CALL VT100M + END + SUBROUTINE RRIBEU(WTR,IDEV,FILEIN,IFAIL) +C AUTHOR: RODGER STADEN + CHARACTER FILEIN*(*) + REAL WTR(10,5) + INTEGER WT(10),TOT(10) + IFAIL=0 +1001 FORMAT(2X,10I3) + CALL OPENRS(IDEV,FILEIN,IOK,LRECL,2) + IF(IOK.NE.0)GO TO 50 +C read space,p + READ(IDEV,1000,ERR=50) + READ(IDEV,1000,ERR=50) +1000 FORMAT() + READ(IDEV,1001,ERR=50)TOT + DO 6 I=1,4 + READ(IDEV,1001,ERR=50)(WT(K),K=1,10) + DO 5 J=1,10 + IF(WT(J).NE.0)THEN + WTR(J,I)=LOG(FLOAT(WT(J))/FLOAT(TOT(J))) + ELSE + WTR(J,I)=LOG(1./FLOAT(TOT(J))) + END IF +5 CONTINUE +6 CONTINUE + CLOSE(UNIT=IDEV) + Z=LOG(1./102.) + DO 40 I=1,10 + WTR(I,5)=Z +40 CONTINUE + RETURN +50 CONTINUE + IFAIL=1 + END + SUBROUTINE FSPLIC(SEQ,IDIM,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,IDEV,DEFFIL,FILNAM, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IDEVOT, + +SUM,WTD,WTA,CEXACD,CEXACA,PEXACD,PEXACA, + +TOT,IDM,MAXLEN,LINE,CHRSET,DIALOG) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),FILNAM*(*),HELPF*(*),DEFFIL*(*) + CHARACTER LINE(MAXLEN),CHRSET(IDM) + INTEGER SUM(IDM,MAXLEN),TOT(MAXLEN),DIALOG,ANSFIL + REAL WTA(IDM,MAXLEN),WTD(IDM,MAXLEN) + INTEGER CEXACD(MAXLEN),PEXACD(MAXLEN) + INTEGER CEXACA(MAXLEN),PEXACA(MAXLEN) + CALL SHOWFU(KBOUT,'Splice search using weight matrix') + CALL GETPAR(62,1,IOK,IGON, + +IPAR2,IPAR3,IPAR4,IPAR5, + +IPAR6,IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + CALL SPLICD(IDEV,DEFFIL,FILNAM, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH, + +SUM,IDM,MAXLEN,TOT,WTD,LENGTD,MIDDLD,YMIND,YMAXD, + +WTA,LENGTA,MIDDLA,YMINA,YMAXA, + +IGON,IDEVOT,LINE,CHRSET,DIALOG,ANSFIL,IOK, + +CEXACD,PEXACD,IEXACD,CEXACA,PEXACA,IEXACA) + IF(IOK.NE.0) RETURN + MARGTD = MARGT/2 + CALL SPLICP(SEQ,IDIM,MARGL,MARGR,MARGB,MARGTD, + +ISXMAX,ISYMAX,J1,J2,IDEVOT,WTD,IDM,MAXLEN,LENGTD, + +YMIND,YMAXD,IGON,MIDDLD,0, + +CEXACD,PEXACD,IEXACD) + MARGBA = MARGB + MARGTD + CALL SPLICP(SEQ,IDIM,MARGL,MARGR,MARGBA,MARGTD, + +ISXMAX,ISYMAX,J1,J2,IDEVOT,WTA,IDM,MAXLEN,LENGTA, + +YMINA,YMAXA,IGON,MIDDLA,1, + +CEXACA,PEXACA,IEXACA) + END + SUBROUTINE SPLICD(IDEV,DEFFIL,FILNAM, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH, + +SUM,IDM,MAXLEN,TOT,WTD,LENGTD,MIDDLD,YMIND,YMAXD, + +WTA,LENGTA,MIDDLA,YMINA,YMAXA, + +IGON,IDEVOT,LINE,CHRSET,DIALOG,ANSFIL,IOK, + +CEXACD,PEXACD,IEXACD,CEXACA,PEXACA,IEXACA) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),HELPF*(*),DEFFIL*(*) + CHARACTER LINE(MAXLEN),CHRSET(IDM) + INTEGER SUM(IDM,MAXLEN),TOT(MAXLEN),DIALOG,ANSFIL + INTEGER CEXACD(MAXLEN),PEXACD(MAXLEN) + INTEGER CEXACA(MAXLEN),PEXACA(MAXLEN) + REAL WTA(IDM,MAXLEN),WTD(IDM,MAXLEN) + IOK = 1 + ANSFIL = 0 + IF(DIALOG.EQ.1) THEN + CALL YESNO(ANSFIL,'Use default weights file', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANSFIL.LT.0) RETURN + END IF + IF(ANSFIL.EQ.1) THEN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + + 'Splice site weight matrix file', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + ELSE + CALL OPENRS(IDEV,DEFFIL,IOK,LRECL,2) + IF(IOK.NE.0) RETURN + END IF + LENGTD=MAXLEN + CALL RDWMTN(TOT,SUM,MIDDLD,LENGTD,MAXLEN,YMIND,YMAXD,IDEV, + + IOK,IDM,KBOUT,0) + IF(IOK.NE.0)THEN + CALL ERROM(KBOUT,'Error in weight matrix') + CLOSE(UNIT=IDEV) + RETURN + END IF + CALL GETWC(TOT,SUM,LENGTD,IDM,MAXLEN,CEXACD,PEXACD,IEXACD) + IF(YMIND.LT.0.0)CALL GETW(TOT,SUM,WTD,LENGTD,IDM,MAXLEN) + IF(YMIND.GE.0.0)CALL GETW2(SUM,WTD,LENGTD,IDM,MAXLEN) + LENGTA=MAXLEN + CALL RDWMTN(TOT,SUM,MIDDLA,LENGTA,MAXLEN,YMINA,YMAXA,IDEV, + + IOK,IDM,KBOUT,0) + CLOSE(UNIT=IDEV) + IF(IOK.NE.0)THEN + CALL ERROM(KBOUT,'Error in weight matrix') + RETURN + END IF + CALL GETWC(TOT,SUM,LENGTA,IDM,MAXLEN,CEXACA,PEXACA,IEXACA) + IF(YMINA.LT.0.0)CALL GETW(TOT,SUM,WTA,LENGTA,IDM,MAXLEN) + IF(YMINA.GE.0.0)CALL GETW2(SUM,WTA,LENGTA,IDM,MAXLEN) +C assume donors in first file: and that the first and last bases in +C exons are marked as middle. Subtract 1 from middla to make frames +C equivalent, but send midc to splicp as a correction so that positions +C of matches are positions on first and last bases in exons. midc=0 for +C donors and 1 for acceptors + MIDDLA = MIDDLA - 1 + IF(DIALOG.EQ.1) THEN + AMN = -9999. + AMX = 9999. + CALL GETRL(AMN,AMX,YMIND,'Donor cutoff score',VAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + YMIND = VAL + CALL GETRL(AMN,AMX,YMINA,'Acceptor cutoff score',VAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + YMINA = VAL + IOK = 1 + CALL YESONO(IGON,'Plot results','List results', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IGON.LT.0)RETURN + END IF + IOK = 0 + END + SUBROUTINE SPLICP(SEQ,IDIM,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,J1,J2,IDEVOT,WT,IDM,MAXLEN,LENGTH, + +YMIN,YMAX,IGON,MIDDLE,MIDC,CEXACT,PEXACT,IEXACT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + REAL WT(IDM,MAXLEN) + INTEGER CEXACT(IEXACT),PEXACT(IEXACT) + INTEGER CTONUM + EXTERNAL CTONUM,MATWTC + XMAX=J2 + XMIN=J1 + IF(IGON.EQ.0)THEN + CALL CLEARV + CALL VECTOM + IMARG = MARGT/3 + CALL FRAME(MARGL,MARGR,MARGB,IMARG,ISXMAX,ISYMAX) + CALL FRAME(MARGL,MARGR,MARGB+IMARG,IMARG,ISXMAX,ISYMAX) + CALL FRAME(MARGL,MARGR,MARGB+(2*IMARG),IMARG,ISXMAX,ISYMAX) + END IF + I = J1 - 1 + 1 CONTINUE + I = I + 1 + IF(IEXACT.GT.0) I = MATWTC(SEQ,IDIM,J1,J2,I,CEXACT,PEXACT,IEXACT) + IF((I+LENGTH-2).LT.J2) THEN + SUM1=0. + K=0 + DO 5 J=I,I+LENGTH-1 + K=K+1 + SUM1=SUM1+WT(CTONUM(SEQ(J)),K) +5 CONTINUE + IF(SUM1.GE.YMIN)THEN + IF(IGON.EQ.0)THEN + M = I + MIDDLE + X = M + MIDC + M = MARGB + (MOD(M,3)*IMARG) + CALL LINE(X,X,YMIN,SUM1,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,M,IMARG,ISXMAX,ISYMAX) + END IF + IF(IGON.EQ.1)THEN + CALL VT100M + M = MOD((I+MIDDLE),3) + WRITE(IDEVOT,1010)I+MIDDLE+MIDC,M,SUM1, + + (SEQ(K),K=I,I+LENGTH-1) +1010 FORMAT(' ',I7,I2,F10.2,' ',120A1) + END IF + END IF + GO TO 1 + END IF + CALL VT100M + END + SUBROUTINE SQCOM2(SEQ,IDIM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),COMP*17 + INTEGER DTONUM + EXTERNAL DTONUM + SAVE COMP + DATA COMP/'AGTC-YRWSKMDVBHN-'/ + DO 100 I=1,IDIM + J = DTONUM(SEQ(I)) + SEQ(I) = COMP(J:J) +100 CONTINUE + END +C SQFIT + SUBROUTINE SQFIT(SEQ,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MIN,ITOTP,KSTART) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) +C ALLOWS R,Y AND - SPECIAL CHARACTERS + IDIF=(IE-IS+2)-IDIM2 +C IDIF IS THE NUMBER OF POSNS TO TRY +C IPSTR GOES FROM 1 TO IDIM2 IDIF TIMES +C TRY ALL POSSIBLE POSITIONS FOR MATCHING AND SCORE FOR EACH +C POINT TO ARRAY ELEMENT CORRESPONDING TO FIRST BASE + IPSEQ=IS-KSTART+1 + ITOTP=0 + DO 200 I=1,IDIF + NTOT=0 + IP=IPSEQ + DO 100 J=1,IDIM2 + IF(SEQ(IP).EQ.STRING(J))THEN + NTOT=NTOT+1 + ELSE IF (STRING(J).EQ.'R')THEN + IF((SEQ(IP).EQ.'A').OR.(SEQ(IP).EQ.'G')) NTOT=NTOT+1 + ELSE IF (STRING(J).EQ.'Y')THEN + IF((SEQ(IP).EQ.'C').OR.(SEQ(IP).EQ.'T')) NTOT=NTOT+1 + ELSE IF (STRING(J).EQ.'-')THEN + NTOT=NTOT+1 + END IF + IP=IP+1 +100 CONTINUE +C END OF COUNTING FOR THIS POSITION.IS TOTAL HIGH ENOUGH? + IF(NTOT.GE.MIN)THEN + ITOTP=ITOTP+1 +C TEST FOR OVERFLOW + IF(ITOTP.GT.ITOTID)RETURN +C SAVE TOTAL AND POSITION + ITOT(ITOTP)=NTOT + ITOTEL(ITOTP)=IP-IDIM2+KSTART-1 + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + END +C SQFIT6 + SUBROUTINE SQFIT6(SEQ,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINS,ITOTP,KSTART) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + INTEGER IUBM1 + EXTERNAL IUBM1 + IDIF=(IE-IS+2)-IDIM2 + IPSEQ=IS-KSTART+1 + ITOTP=0 + DO 200 I=1,IDIF + NTOT = 0 + IP=IPSEQ + DO 100 J=1,IDIM2 + NTOT = NTOT + IUBM1(STRING(J),SEQ(IP)) + IP=IP+1 +100 CONTINUE + IF(NTOT.GE.MINS)THEN + ITOTP=ITOTP+1 + IF(ITOTP.GT.ITOTID)RETURN + ITOT(ITOTP)=NTOT + ITOTEL(ITOTP)=IP-IDIM2+KSTART-1 + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + END + SUBROUTINE SRCHP(SEQ,IDIM1, + +MARGL,MARGR,MARGB,MARGT,ISXMAX, + +ISYMAX,J1,J2,KBOUT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(6) + SAVE STRING + DATA STRING/'A','A','T','A','A','A'/ + CALL SHOWFU(KBOUT,'PolyA search. i.e. AATAAA search') + XMIN=J1 + XMAX=J2 + YMIN=0. + YMAX=MARGT + Y0=0. + IBH=MARGT + CALL VECTOM + CALL SRCHP1(SEQ,IDIM1,J1,J2,STRING,6,1, + +XMAX,XMIN,YMAX,YMIN,MARGL,MARGR,MARGB,MARGT,ISXMAX, + +ISYMAX,Y0,IBH) + CALL VT100M + END + SUBROUTINE SRCHP1(SEQ,IDIM1,J1,J2,STRING,IDIMS,INC, + +XMAX,XMIN,YMAX,YMIN,MARGL,MARGR,MARGB,MARGT,ISXMAX, + +ISYMAX,Y,IBH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(6) + YP=Y+IBH + IAT=J1-INC +10 CONTINUE + IAT=IAT+INC + IDIM=J2-IAT+1 + IF(IDIM.LE.0)RETURN + CALL FIND6(SEQ(IAT),IDIM,STRING,IDIMS,INC,JMATCH) + IF(JMATCH.EQ.0)RETURN + IAT=IAT+JMATCH-1 + X=IAT + CALL LINE(X,X,YP,Y,XMAX,XMIN,YMAX,YMIN,MARGL,MARGR,MARGB, + +MARGT,ISXMAX,ISYMAX) + GO TO 10 + END + SUBROUTINE STOPSB(SEQ,IDIM1,J1,J2,MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX,PAA) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),PAA(5,5,5) + IMARG=NINT(MARGT/2.) + MARGB3=MARGB + MARGB5=MARGB+IMARG + MARGT3=IMARG + MARGT5=IMARG + CALL STOPS(SEQ,IDIM1,J1,J2, + +MARGL,MARGR,MARGB5,MARGT5, + +ISXMAX,ISYMAX,PAA) + CALL STOPSC(SEQ,IDIM1,J1,J2,MARGL,MARGR, + +MARGB3,MARGT3, + +ISXMAX,ISYMAX,PAA) + END +C +C STOPS + SUBROUTINE STOPS(SEQ,IDIM1,J1,J2, + +MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX,PAA) +C AUTHOR: RODGER STADEN + CHARACTER STOP(3),SEQ(IDIM1),PAA(5,5,5),BASE(5) + SAVE BASE + DATA BASE/'T','C','A','G','-'/ + CALL VECTOM + XMIN=J1 + XMAX=J2 + YMIN=0. +C DIVIDE SCREEN INTO 3 STRIPS AND PUT BLIPS AT 1/6,3/6,5/6 + YMAX=ISYMAX-(MARGB1+MARGT1) + YMAX3=YMAX/3. + BLIPH=YMAX*0.02 + DO 10 ICOD=1,5 + DO 10 JCOD=1,5 + DO 10 KCOD=1,5 + IF(PAA(KCOD,JCOD,ICOD).EQ.'*')THEN +C NEED TO KNOW THE CODON + STOP(1)=BASE(ICOD) + STOP(2)=BASE(JCOD) + STOP(3)=BASE(KCOD) + BLIPF=YMAX3/2.0 + CALL PSRCH(SEQ,IDIM1,J1,J2,STOP,3,3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX,BLIPF,BLIPH) + BLIPF=BLIPF+YMAX3 + J11=J1+1 + CALL PSRCH(SEQ,IDIM1,J11,J2,STOP,3,3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX,BLIPF,BLIPH) + BLIPF=BLIPF+YMAX3 + J11=J1+2 + CALL PSRCH(SEQ,IDIM1,J11,J2,STOP,3,3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX,BLIPF,BLIPH) + END IF +10 CONTINUE + CALL VT100M + END +C STOPSC + SUBROUTINE STOPSC(SEQ,IDIM1,J1,J2, + +MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX,PAA) +C AUTHOR: RODGER STADEN + CHARACTER STOP(3),SEQ(IDIM1),PAA(5,5,5),BASE(5) + SAVE BASE + DATA BASE/'A','G','T','C','-'/ + CALL VECTOM + XMIN=J1 + XMAX=J2 + YMIN=0. +C DIVIDE SCREEN INTO 3 STRIPS AND PUT BLIPS AT 1/12,5/12,9/12 + YMAX=ISYMAX-(MARGB1+MARGT1) + YMAX3=YMAX/3. + BLIPH=YMAX*0.02 + DO 10 ICOD=1,5 + DO 10 JCOD=1,5 + DO 10 KCOD=1,5 + IF(PAA(KCOD,JCOD,ICOD).EQ.'*')THEN +C NEED TO KNOW THE CODON + STOP(1)=BASE(KCOD) + STOP(2)=BASE(JCOD) + STOP(3)=BASE(ICOD) + BLIPF=YMAX3/4.0 + CALL PSRCH(SEQ,IDIM1,J1,J2,STOP,3,3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX,BLIPF,BLIPH) + BLIPF=BLIPF+YMAX3 + J11=J1+1 + CALL PSRCH(SEQ,IDIM1,J11,J2,STOP,3,3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX,BLIPF,BLIPH) + BLIPF=BLIPF+YMAX3 + J11=J1+2 + CALL PSRCH(SEQ,IDIM1,J11,J2,STOP,3,3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX,BLIPF,BLIPH) + END IF +10 CONTINUE + CALL VT100M + END +C +C STARTS + SUBROUTINE STARTS(SEQ,IDIM1,J1,J2, + +MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX,PAA) +C AUTHOR: RODGER STADEN + CHARACTER CODON(3),SEQ(IDIM1),PAA(5,5,5),BASE(5) + SAVE BASE + DATA BASE/'T','C','A','G','-'/ + CALL VECTOM + XMIN=J1 + XMAX=J2 +C DIVIDE SCREEN INTO 3 STRIPS AND PUT BLIPS AT 0.0,1/3,2/3 + YMAX=ISYMAX-(MARGB1+MARGT1) + YMAX3=YMAX/3. + BLIPH=YMAX*0.013 + YMIN=0. + DO 10 ICOD=1,5 + DO 10 JCOD=1,5 + DO 10 KCOD=1,5 + IF(PAA(KCOD,JCOD,ICOD).EQ.'M')THEN +C NEED TO KNOW THE CODON + CODON(1)=BASE(ICOD) + CODON(2)=BASE(JCOD) + CODON(3)=BASE(KCOD) + BLIPF=0.0 + CALL PSRCH(SEQ,IDIM1,J1,J2,CODON,3,3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX,BLIPF,BLIPH) + BLIPF=BLIPF+YMAX3 + J11=J1+1 + CALL PSRCH(SEQ,IDIM1,J11,J2,CODON,3,3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX,BLIPF,BLIPH) + BLIPF=BLIPF+YMAX3 + J11=J1+2 + CALL PSRCH(SEQ,IDIM1,J11,J2,CODON,3,3,XMAX,XMIN,YMAX,YMIN, + + MARGL1,MARGR1,MARGB1,MARGT1,ISXMAX,ISYMAX,BLIPF,BLIPH) + END IF +10 CONTINUE + CALL VT100M + END + CHARACTER*1 FUNCTION SCOMP(CHAR1) +C AUTHOR: RODGER STADEN + CHARACTER CHAR1,CHARSU(5),CHARSL(5) + INTEGER CTONUM + EXTERNAL CTONUM,ICOMP + SAVE CHARSU,CHARSL + DATA CHARSU/'T','C','A','G','-'/ + DATA CHARSL/'t','c','a','g','-'/ + JCHAR=CTONUM(CHAR1) + IF(CHAR1.EQ.CHARSU(JCHAR)) THEN + SCOMP=CHARSU(ICOMP(JCHAR)) + ELSE + SCOMP=CHARSL(ICOMP(JCHAR)) + END IF + END +C TRANS +C SUBROUTINE TO TRANSLATE A SEQUENCE IN 1 PHASE GIVEN START & STOP +C POSITIONS AND A SEQUENCE + SUBROUTINE TRANS(SEQNCE,IDIM1,IS,IE,OUTP,PAA,IDIM2,KSTART, + +JSTRAN) +C AUTHOR: RODGER STADEN + CHARACTER SEQNCE(IDIM1),PAA(125),OUTP(IDIM2),CODON(3),TRANF,TRANB + INTEGER POUT + EXTERNAL LWRAP,TRANF,TRANB + IX2=IE +C ADD ON TO END IF OVER JOIN. REALLY A COUNTER OF +C NUMBER OF ELEMENTS TO TRANSLATE. LWRAP TAKES CARE OF OVERFLOW. +C WRAP AROUND IF OVER JOIN + IF(IX2.LE.IS)IX2=IX2+IDIM1 + DO 500 I=IS-KSTART+1,IX2-KSTART+1,3 + DO 400 J=1,3 + IP1=I+J-1 + IP1=LWRAP(IDIM1,IP1) + CODON(J)=SEQNCE(IP1) +400 CONTINUE + IP=I + IP=LWRAP(IDIM1,IP) + POUT=IP/3+1 + IF(JSTRAN.EQ.0)OUTP(POUT)=TRANF(CODON,PAA) + IF(JSTRAN.EQ.1)OUTP(POUT)=TRANB(CODON,PAA) +500 CONTINUE + END + SUBROUTINE TRANSD(SEQNCE,IDIM1,IS,IE,OUTP,IDIM2,PAA,JSTRAN) +C AUTHOR: RODGER STADEN + CHARACTER SEQNCE(IDIM1),PAA(125),OUTP(IDIM2),CODON(3),TRANF,TRANB + INTEGER POUT + EXTERNAL JCODNO,TRANF,TRANB,LWRAP + POUT=0 +C SET UP LOOP VALUE + IX2=IE +C ADD ON TO END IF OVER JOIN. REALLY A COUNTER OF +C NUMBER OF ELEMENTS TO TRANSLATE. LWRAP TAKES CARE OF OVERFLOW. +C WRAP AROUND IF OVER JOIN + IF(IX2.LE.IS)IX2=IX2+IDIM1 + DO 500 I=IS,IX2,3 + DO 400 J=1,3 + IP1=I+J-1 + IP1=LWRAP(IDIM1,IP1) + CODON(J)=SEQNCE(IP1) +400 CONTINUE + POUT=POUT+1 + IF(JSTRAN.EQ.0)OUTP(POUT)=TRANF(CODON,PAA) + IF(JSTRAN.EQ.1)OUTP(POUT)=TRANB(CODON,PAA) +500 CONTINUE + IDIM2=POUT + END + INTEGER FUNCTION JCODNO(SEQ,IDIM,I) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + INTEGER CTONUM + EXTERNAL CTONUM,LWRAP +C DECODE NEXT 3 CHARS TO GIVE POINTERS TO AMINO ACIDS + J0=I + J0=LWRAP(IDIM,J0) + J1=LWRAP(IDIM,J0+1) + J2=LWRAP(IDIM,J1+1) + K0=CTONUM(SEQ(J0))*25 + K1=CTONUM(SEQ(J1))*5 + K2=CTONUM(SEQ(J2)) + JCODNO=K0+K1+K2-30 + END + SUBROUTINE OPENTR(SEQ,IDIM,KSTART,IDEV,KBIN,KBOUT,PAA, + +OUTP,IDIMP,FRAMEC,POSNS,MAXPS,IDEVE,FILNAM, + +IHELPS,IHELPE,HELPF,IDEVH,DIALOG) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIMP),PAA(125),OUTP(IDIMP) + CHARACTER HELPF*(*),FILNAM*(*),OPRATR*20 + INTEGER FRAMEC(IDIMP),POSNS(MAXPS),ANSE,DIALOG + CALL SHOWFU(KBOUT, + +'Find open reading frames, translate and list') + CALL GETPAR(39,10,IOK,MINO,MINOPN,JSTRAN, + +MINLEN,MAXLEN,LINLEN,ITRAN,JTRAN,INUM,ANSE, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + MAXPOS = MAXPS + MAXO = IDIM/3 + I1 = 1 + I2 = IDIM + IF(DIALOG.EQ.1)CALL TRAND7(KBIN,KBOUT,I1,I2,ITRAN, + +MINLEN,MAXLEN,LINLEN, + +MINO,MAXO,MINOPN,JSTRAN,INUM,IDEVE,ANSE,JTRAN,FILNAM, + +IOK,OPRATR, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL FILLI(FRAMEC,IDIM,1) + SEQ(IDIM+1) = SEQ(1) + SEQ(IDIM+2) = SEQ(2) + IF(JTRAN.EQ.0) THEN + IF(ANSE.EQ.1) THEN + CALL TRANEM(SEQ,IDIM,I1,I2,FRAMEC, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + ELSE IF(ANSE.EQ.2) THEN + CALL TRANEN(SEQ,IDIM,I1,I2,FRAMEC,JSTRAN,IDEVE, + + POSNS,MAXPOS,KBOUT,OPRATR,IOK) + ELSE + IF((JSTRAN.EQ.0).OR.(JSTRAN.EQ.1))THEN + CALL TRPIRP(SEQ,IDIM,I1,I2,KSTART,MINOPN,IDEV,PAA, + + JSTRAN,OUTP,FRAMEC,IDIMP) + ELSE IF (JSTRAN.EQ.2) THEN + CALL TRPIRP(SEQ,IDIM,I1,I2,KSTART,MINOPN,IDEV,PAA, + + 0,OUTP,FRAMEC,IDIMP) + CALL TRPIRP(SEQ,IDIM,I1,I2,KSTART,MINOPN,IDEV,PAA, + + 1,OUTP,FRAMEC,IDIMP) + END IF + END IF + END IF + CALL TRAN6X(SEQ,IDIMP,IDEV,I1,I2,PAA,ITRAN,JSTRAN,INUM,LINLEN, + +FRAMEC) + END + SUBROUTINE TRANEM(SEQ,IDIM,J1,J2,FRAMEC, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) +C get start and end points for translation, then set up +C the integer array for tran6x + CHARACTER SEQ(IDIM) + INTEGER FRAMEC(IDIM),FRAME + KSTRAN = 0 + 50 CONTINUE + KSTRAN = 0 + CALL TRAND8(IDIM,J1,J2,KSTRAN,IP1,IP2,'Translate', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + IF(IP1.EQ.0) RETURN + FRAME = MOD(IP1,3) + IF(FRAME.EQ.0) FRAME = 3 + IF(KSTRAN.EQ.0) THEN + CALL MBPRIM(FRAMEC,IDIM,IP1,IP2,2) + ELSE + CALL MBPRIM(FRAMEC,IDIM,IP1,IP2,3) + END IF + GO TO 50 + END + SUBROUTINE TRAND8(IDIM,J1,J2,KSTRAN,IP1,IP2,PROMPT, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) +C get start and end points and strand for translation + CHARACTER PROMPT*(*),HELPF*(*) + CALL GETRC2(KBIN,KBOUT,J1,J2,IDIM,IP1,IP2,PROMPT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF(IP1.EQ.0) RETURN + IOK = 1 + IOP = KSTRAN + CALL YESONO(IOP,'+ strand','- strand', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) RETURN + KSTRAN = IOP + IOK = 0 + END + SUBROUTINE TRAND7(KBIN,KBOUT,I1,I2, + +ITRAN,MINLEN,MAXLEN,LINLEN,MINO,MAXO,MINOPN,JSTRAN,INUM, + +IDEVE,ANSE,JTRAN,FILNAM,IOK,OPRATR, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN +C organise user interaction for translation to screen + CHARACTER HELPF*(*),FILNAM*(*) + PARAMETER (MAXPRM = 25) + CHARACTER PROMPT(4)*(MAXPRM),OPRATR*(*) + INTEGER ANSE +C Modified 11-4-91 to make 6 phase translation more obvious +C Translate if JTRAN = 0 +C Keyboard input if ANSE = 1 +C feature table input if ANSE = 2 +C open reading frames is ANSE = 3 +C + strand if JSTRAN = 0 +C - strand if JSTRAN = 1 +C both strands JSTRAN = 2 +C one letter codes if ITRAN = 0 +C three letter codes if ITRAN = 3 +C number ends of lines if INUM = 0 +C Display I1 to I2 + IOK = 1 + J1 = I1 + J2 = I2 + IOP = 0 + CALL YESONO(IOP,'Show translation','Hide translation', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) THEN + IOK = 1 + RETURN + END IF + JTRAN = IOP + IF(JTRAN.EQ.0) THEN + PROMPT(1) = 'Typed on the keyboard' + PROMPT(2) = 'Read from a feature table' + PROMPT(3) = 'Open reading frames' + PROMPT(4) = 'All six frames' + CALL RADION('The segments to translate can be', + + PROMPT,4,ANSE, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANSE.LT.1) RETURN + IF(ANSE.EQ.2) THEN + CALL TRAND9(KBIN,KBOUT,IDEVE,FILNAM,OPRATR, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + IF(ANSE.EQ.3) THEN + CALL GETINT(MINO,MAXO,MINOPN, + + 'Minimum open frame in amino acids', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINOPN = IVAL + END IF + IF(ANSE.EQ.4) THEN + MINOPN = 0 + JSTRAN = 2 + END IF + IOP = ITRAN + IF(IOP.EQ.3) IOP = 1 + CALL YESONO(IOP,'Use 1 letter codes', + + 'Use 3 letter codes', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) THEN + IOK = 1 + RETURN + END IF + IF(IOP.EQ.1) ITRAN = 3 + IF(IOP.EQ.0) ITRAN = 0 + END IF + CALL GTREG(KBIN,KBOUT,J1,J2,I1,I2, + +'Define section of DNA to display', + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL GETINT(MINLEN,MAXLEN,LINLEN,'Line length', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + LINLEN = IVAL + IF(JTRAN.EQ.0) THEN + IF(MOD(LINLEN,3).NE.0) LINLEN = 60 + END IF + IF(ANSE.NE.4) THEN + IVAL = JSTRAN + 1 + CALL GSTRND(IVAL,IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IVAL.LT.1) THEN + IOK = 1 + RETURN + END IF + JSTRAN = IVAL - 1 + END IF + IOP = INUM + CALL YESONO(IOP,'Number ends of lines', + +'Number every tenth base', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) THEN + IOK = 1 + RETURN + END IF + INUM = IOP + IOK = 0 + END + SUBROUTINE EMBLF(IDEVE,FILNAM, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,ANSE,OPRATR,IOK) +C AUTHOR: RODGER STADEN +C ask about keyboard or ft input. If ft open file and anse = 1 + CHARACTER FILNAM*(*),HELPF*(*),OPRATR*(*) + INTEGER ANSE + IOK = 1 + IOP = ANSE + CALL YESONO(IOP,'Define segments using keyboard', + +'Define segments using embl feature table', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) RETURN + ANSE = IOP + IF(ANSE.EQ.1) THEN + CALL TRAND9(KBIN,KBOUT,IDEVE,FILNAM,OPRATR, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + IOK = 0 + END + SUBROUTINE TRAND9(KBIN,KBOUT,IDEVE,FILNAM,OPRATR, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C open feature table file and get ft operator + CHARACTER FILNAM*(*),HELPF*(*) + CHARACTER OPRATR*(*) + FILNAM = ' ' + CALL OPENF1(IDEVE,FILNAM,0,IOK,KBIN,KBOUT, + +'Feature table file name', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + 20 CONTINUE + IOK = 1 + LIN = 3 + CALL GTSTR('Operator','all',OPRATR,LIN, + +KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) RETURN + CALL CCASE(OPRATR,1) + IF((LIN.EQ.0).OR.(OPRATR(1:3).EQ.'ALL')) OPRATR(1:1) = ' ' + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 20 + END IF + IOK = 0 + END + SUBROUTINE TRANEN(SEQ,IDIM,J1,J2,FRAMEC,JSTRAN,IDEVE, + +POSNS,MAXPOS,KBOUT,OPRATR,IOK) +C use ft to set up array for tran6x + CHARACTER SEQ(IDIM),OPRATR*(*) + INTEGER POSNS(MAXPOS),FRAMEC(IDIM) + INTEGER EMBLFT + EXTERNAL EMBLFT + ISTRAN = 0 + IF(JSTRAN.EQ.1) ISTRAN = 1 +10 CONTINUE + NOBJ = 0 + IOK = EMBLFT(IDEVE,KBOUT,'CDS',OPRATR,ISTRAN, + +POSNS,MAXPOS,NPOS,NOBJ) + IF(IOK.NE.0) THEN + CLOSE(UNIT=IDEVE) + RETURN + END IF + IF(ISTRAN.EQ.0) THEN + K = NPOS + DO 100 I=1,NOBJ + NSEG = POSNS(K) + IADD = 0 + ITOT = 0 + DO 50 J=K-NSEG,K-1,2 + IP1 = POSNS(J) + IP2 = POSNS(J+1) + ITOT = ITOT + IP2 - IP1 + 1 + IP1 = IP1 - IADD + CALL MBPRIM(FRAMEC,IDIM,IP1,IP2,2) + IADD = MOD(ITOT,3) + 50 CONTINUE + K = K - NSEG - 1 + 100 CONTINUE + ELSE + K = NPOS + DO 200 I=1,NOBJ + NSEG = POSNS(K) + IADD = 0 + ITOT = 0 + DO 60 J=K-1,K-NSEG,-2 + IP1 = POSNS(J-1) + IP2 = POSNS(J) + ITOT = ITOT + IP2 - IP1 + 1 + IP2 = IP2 + IADD +C mbprim expect complementary strand codon positions to start +C from the same position as their counterparts on the other strand +C ie the first position should correspond to the third +C + strand 123 +C - strand 123 + CALL MBPRIM(FRAMEC,IDIM,IP1,IP2,-3) + IADD = MOD(ITOT,3) + 60 CONTINUE + K = K - NSEG - 1 + 200 CONTINUE + END IF + IF((JSTRAN.EQ.2).AND.(ISTRAN.EQ.0)) THEN + ISTRAN = 1 + REWIND(IDEVE) + GO TO 10 + END IF + CLOSE(UNIT=IDEVE) + END + SUBROUTINE TRAND1(JSTRAN,ANSE,IDEVE,FILNAM, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,OPRATR,IOK) +C AUTHOR: RODGER STADEN +C ask for ft or kb input, get operator and strand + INTEGER ANSE + CHARACTER FILNAM*(*),HELPF*(*),OPRATR*(*) + IOK = 1 + CALL EMBLF(IDEVE,FILNAM, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,ANSE,OPRATR,IOK) + IF(IOK.NE.0)RETURN + IF(ANSE.EQ.1)THEN + IOP = JSTRAN + 1 + CALL GSTRND(IOP,IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.1) THEN + IOK = 1 + RETURN + END IF + JSTRAN = IOP - 1 + END IF + IOK = 0 + END + SUBROUTINE TRANDK(SEQ1,IDIM1,OUTP1,IDIMP,ISTART,ISTOP,IDIMT, + +IDEVE,IDEV,FILNAM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PAA,J1,J2,POSNS,MAXPOS) + CHARACTER HELPF*(*),OPRATR*20 + INTEGER ANSE,POSNS(MAXPOS) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM1),OUTP1(IDIMP),PAA(125),FILNAM*(*) +C J1 IS THE FIRST BASE IN THE RAM BUFFER +C J2 IS THE LAST BASE IN THE RAM BUFFER +C IDIMT IS THE ACTUAL SEQUENCE LENGTH +C WRAP AROUND IS ONLY POSSIBLE IF J1=1, AND J2=IDIMT + CALL SHOWFU(KBOUT,'Translate and write protein sequence to disk') + CALL GETPAR(40,7,IOK,JSTRAN,ANSE,IOPEN,MINO,MAXO,MINOPN,JSTRAN, + +IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IOP = IOPEN + CALL YESONO(IOP,'Translate selected regions', + +'Translate open reading frames', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) RETURN + IF(IOP.EQ.1) THEN + CALL TRNPIR(SEQ1,IDIM1,J1,J2,ISTART,KBIN,KBOUT,IDEVE,PAA, + + OUTP1,IDIMP,FILNAM,MINO,MAXO,MINOPN,JSTRAN, + + IHELPS,IHELPE,HELPF,IDEVH) + RETURN + END IF + CALL TRAND1(JSTRAN,ANSE,IDEVE,FILNAM, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,OPRATR,IOK) + IF(IOK.NE.0) THEN + IF(ANSE.EQ.1) CLOSE(UNIT=IDEVE) + RETURN + END IF + IF(ANSE.EQ.1) THEN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,1,IOK,KBIN,KBOUT, + + 'Output file name', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL TRNEMB(SEQ1,IDIM1,J1,J2,OUTP1,PAA,JSTRAN,IDEVE,IDEV, + + POSNS,MAXPOS,KBOUT,OPRATR,IOK) + RETURN + END IF + IOUT = -4 + CALL FILLC(OUTP1,IDIMP,'-') +C +50 CONTINUE +C + CALL TRAND8(IDIMT,J1,J2,JSTRAN,I1,N1,'Translate', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + IF(I1.NE.0) THEN + IB = N1 - I1 + 1 + IF(N1.LT.I1) IB = IB + IDIM1 + IAA = IB/3 + 1 + IF(IAA.GE.2) THEN + IOUT = IOUT + 5 + CALL TRANSD(SEQ1,IDIM1, + + I1-ISTART+1,N1-ISTART+1,OUTP1(IOUT),IAA,PAA,JSTRAN) + IF(JSTRAN.EQ.1) CALL SQREV(OUTP1(IOUT),IAA) + IOUT = IOUT + IAA - 1 + END IF + GO TO 50 + END IF + IF(IOUT.GT.0) THEN + FILNAM = ' ' + CALL OPENF1(IDEVE,FILNAM,1,IOK,KBIN,KBOUT, + + 'Output file name', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL FMTDKN(IDEVE,OUTP1,IOUT) + CLOSE(UNIT=IDEVE) + RETURN + END IF + END + SUBROUTINE TRNEMB(SEQ,IDIM,J1,J2,SEQW,PAA,JSTRAN,IDEVE,IDEV, + +POSNS,MAXPOS,KBOUT,OPRATR,IOK) +C translate to disk using ft + CHARACTER SEQ(IDIM),OPRATR*(*),SEQW(IDIM),PAA(125),LINE*60 + INTEGER POSNS(MAXPOS) + INTEGER EMBLFT,WPAIR + EXTERNAL EMBLFT,ITOSL,WPAIR + ISTRAN = 0 + IF(JSTRAN.EQ.1) ISTRAN = 1 +10 CONTINUE + NOBJ = 0 + IOK = EMBLFT(IDEVE,KBOUT,'CDS',OPRATR,ISTRAN, + +POSNS,MAXPOS,NPOS,NOBJ) + IF(IOK.NE.0) THEN + CLOSE(UNIT=IDEVE) + RETURN + END IF + IF(ISTRAN.EQ.0) THEN + K = NPOS + DO 100 I=1,NOBJ + NSEG = POSNS(K) + IB = 1 + IF((POSNS(K-NSEG).GE.J1).AND.(POSNS(K-1).LE.J2)) THEN + DO 50 J=K-NSEG,K-1,2 + L = POSNS(J+1) - POSNS(J) + 1 + CALL SQCOPY(SEQ(POSNS(J)),SEQW(IB),L) + IB = IB + L + 1001 FORMAT(A) + 50 CONTINUE + IB = IB - 1 + IACIDS = IB/3 + 1 + CALL TRANSD(SEQW,IDIM,1,IB,SEQW,IACIDS,PAA,ISTRAN) + LINE(1:) = '>' + IF(ITOSL(LINE(2:),POSNS(K-NSEG)).EQ.0) + + WRITE(*,*)'Scream: ITOSL' + IF(WPAIR(LINE(22:),POSNS(K-NSEG),POSNS(K-1)).NE.0) + + WRITE(*,*)'Scream: WPAIR' + WRITE(IDEV,1001)LINE + IF (SEQW(IACIDS).NE.'*') THEN + IACIDS = IACIDS + 1 + SEQW(IACIDS) = '*' + END IF + CALL FMTDKN(IDEV,SEQW,IACIDS) + END IF + K = K - NSEG - 1 + 100 CONTINUE + ELSE + K = NPOS + DO 200 I=1,NOBJ + NSEG = POSNS(K) + IB = 1 + IF((POSNS(K-NSEG).GE.J1).AND.(POSNS(K-1).LE.J2)) THEN + DO 60 J=K-NSEG,K-1,2 + L = POSNS(J+1) - POSNS(J) + 1 + CALL SQCOPY(SEQ(POSNS(J)),SEQW(IB),L) + IB = IB + L + 60 CONTINUE + IB = IB - 1 + CALL SQREV(SEQW,IB) + CALL SQCOM2(SEQW,IB) + IACIDS = IB/3 + 1 + CALL TRANSD(SEQW,IDIM,1,IB,SEQW,IACIDS,PAA,0) + IF (SEQW(IACIDS).NE.'*') THEN + IACIDS = IACIDS + 1 + SEQW(IACIDS) = '*' + END IF + LINE(1:) = '> complement(' + IF (ITOSL(LINE(2:),POSNS(K-1)).EQ.0) WRITE(*,*)'Scream: ITOSL' + IF(WPAIR(LINE(33:),POSNS(K-NSEG),POSNS(K-1)).NE.0) + + WRITE(*,*)'Scream: WPAIR' + LINE(32+INDEX(LINE(33:),' '):) = ')' + WRITE(IDEV,1001)LINE + CALL FMTDKN(IDEV,SEQW,IACIDS) + END IF + K = K - NSEG - 1 + 200 CONTINUE + END IF + IF((JSTRAN.EQ.2).AND.(ISTRAN.EQ.0)) THEN + ISTRAN = 1 + REWIND(IDEVE) + GO TO 10 + END IF + CLOSE(UNIT=IDEVE) + CLOSE(UNIT=IDEV) + END + SUBROUTINE CODTDK(SEQ,IDIM,J1,J2,IDEVE,FILNAM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PAA,POSNS,MAXPOS,SEQW) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),FILNAM*(*),PAA(5,5,5),OPRATR*20 + REAL S1(64),S2(64) + CHARACTER SEQW(IDIM) + INTEGER POSNS(MAXPOS) + INTEGER NORM,ANSE,ANST,ANSTO + CALL SHOWFU(KBOUT,'Calculate codon table and write it to disk') + CALL GETPAR(41,5,IOK,JSTRAN,NORM,ANSE,ANST,ANSTO, + +IPAR6,IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + JSTRAN = 0 + NORM = 0 + ANSE = 0 + ANST = 0 + ANSTO = 0 +C ANSTO REDUNDANT + CALL FILLR(S2,64,0.0) + CALL CODTBL(IDEVE,FILNAM,S2, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,ANST,IOK) + IF(IOK.NE.0) RETURN + CALL CODND1(JSTRAN,NORM,ANSE,IDEVE,FILNAM,OPRATR, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + IF(ANSE.EQ.1) THEN + CALL CTDEMB(SEQ,IDIM,J1,J2,SEQW,PAA,JSTRAN,IDEVE, + + POSNS,MAXPOS,KBIN,KBOUT,OPRATR,S1,S2,NORM,FILNAM,IOK) + RETURN + END IF +100 CONTINUE + CALL FILLR(S1,64,0.0) + CALL TRAND8(IDIM,J1,J2,JSTRAN,I1,I2,'Count over', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + IF(I1.EQ.0) GO TO 200 + IDIM1 = I2-I1+1 + CALL CALCOD(S1,SEQ(I1),IDIM1) + IF(JSTRAN.EQ.1)CALL CODCOM(S1) + CALL ADDR(S1,S2,64) + IF(NORM.EQ.1)CALL NORMAA(S1,100.,PAA) + CALL WRTCOD(S1,KBOUT,PAA) + GO TO 100 +200 CONTINUE + WRITE(KBOUT,9991) +9991 FORMAT(5X,'Codon totals over all genes') + IF(NORM.EQ.1) CALL NORMAA(S2,100.,PAA) + CALL WRTCOD(S2,KBOUT,PAA) + FILNAM = ' ' + CALL OPENF1(IDEVE,FILNAM,1,IOK,KBIN,KBOUT, + + 'Name for codon table file', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL WRTCOD(S2,IDEVE,PAA) + CLOSE(UNIT=IDEVE) + END + SUBROUTINE CODND1(JSTRAN,NORM,ANSE,IDEVE,FILNAM,OPRATR, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) +C AUTHOR: RODGER STADEN +C get observed or normalised, kb or ft, operator, strand + INTEGER ANSE + CHARACTER FILNAM*(*),HELPF*(*),OPRATR*(*) + IOK = 1 + IOP = NORM + CALL YESONO(IOP,'Show observed counts', + +'Normalize counts', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOP.LT.0) RETURN + NORM = IOP + CALL TRAND1(JSTRAN,ANSE,IDEVE,FILNAM, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,OPRATR,IOK) + IF(IOK.NE.0) THEN + IF(ANSE.EQ.1) CLOSE(UNIT=IDEVE) + RETURN + END IF + IOK = 0 + END + SUBROUTINE CTDEMB(SEQ,IDIM,J1,J2,SEQW,PAA,JSTRAN,IDEVE, + +POSNS,MAXPOS,KBIN,KBOUT,OPRATR,S1,S2,NORM,FILNAM,IOK) +C codons to disk using ft + CHARACTER SEQ(IDIM),OPRATR*(*),SEQW(IDIM),PAA(125) + INTEGER POSNS(MAXPOS) + REAL S1(64),S2(64) + CHARACTER FILNAM*(*) + INTEGER EMBLFT + EXTERNAL EMBLFT + ISTRAN = 0 + IF(JSTRAN.EQ.1) ISTRAN = 1 +10 CONTINUE + NOBJ = 0 + IOK = EMBLFT(IDEVE,KBOUT,'CDS',OPRATR,ISTRAN, + +POSNS,MAXPOS,NPOS,NOBJ) + IF(IOK.NE.0) THEN + CLOSE(UNIT=IDEVE) + RETURN + END IF + IF(ISTRAN.EQ.0) THEN + K = NPOS + DO 100 I=1,NOBJ + NSEG = POSNS(K) + IB = 1 + IF((POSNS(K-NSEG).GE.J1).AND.(POSNS(K-1).LE.J2)) THEN + WRITE(KBOUT,*)'Data from end point ',POSNS(K-NSEG) + DO 50 J=K-NSEG,K-1,2 + L = POSNS(J+1) - POSNS(J) + 1 + CALL SQCOPY(SEQ(POSNS(J)),SEQW(IB),L) + IB = IB + L +C note not checking for within j1,j2 + 50 CONTINUE + IB = IB - 1 + IB = 3*(IB/3) + CALL FILLR(S1,64,0.0) + CALL CALCOD(S1,SEQW,IB) + CALL ADDR(S1,S2,64) + IF(NORM.EQ.1)CALL NORMAA(S1,100.,PAA) + CALL WRTCOD(S1,KBOUT,PAA) + END IF + K = K - NSEG - 1 + 100 CONTINUE + ELSE + K = NPOS + DO 200 I=1,NOBJ + NSEG = POSNS(K) + IB = 1 + IF((POSNS(K-NSEG).GE.J1).AND.(POSNS(K-1).LE.J2)) THEN + WRITE(KBOUT,*)'Data from end point ',POSNS(K-1) + DO 60 J=K-NSEG,K-1,2 + L = POSNS(J+1) - POSNS(J) + 1 + CALL SQCOPY(SEQ(POSNS(J)),SEQW(IB),L) + IB = IB + L + 60 CONTINUE + IB = IB - 1 + CALL SQREV(SEQW,IB) + CALL SQCOM2(SEQW,IB) + IB = 3*(IB/3) + CALL FILLR(S1,64,0.0) + CALL CALCOD(S1,SEQW,IB) + CALL ADDR(S1,S2,64) + IF(NORM.EQ.1)CALL NORMAA(S1,100.,PAA) + CALL WRTCOD(S1,KBOUT,PAA) + END IF + K = K - NSEG - 1 + 200 CONTINUE + END IF + IF((JSTRAN.EQ.2).AND.(ISTRAN.EQ.0)) THEN + ISTRAN = 1 + REWIND(IDEVE) + GO TO 10 + END IF + CLOSE(UNIT=IDEVE) + WRITE(KBOUT,9991) +9991 FORMAT(5X,'Codon totals over all genes') + IF(NORM.EQ.1) CALL NORMAA(S2,100.,PAA) + CALL WRTCOD(S2,KBOUT,PAA) + FILNAM = ' ' + CALL OPENF1(IDEVE,FILNAM,1,IOK,KBIN,KBOUT, + + 'Name for codon table file', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL WRTCOD(S2,IDEVE,PAA) + CLOSE(UNIT=IDEVE) + END + SUBROUTINE CODEMB(SEQ,IDIM,J1,J2,SEQW,PAA,JSTRAN,IDEVE,IDEV, + +POSNS,MAXPOS,KBIN,KBOUT,OPRATR,S1,S2,S3,NORM,FILNAM,IOK) +C codons, acids, bases to screen using ft + CHARACTER SEQ(IDIM),OPRATR*(*),SEQW(IDIM),PAA(125) + INTEGER POSNS(MAXPOS) + REAL S1(64),S2(64),S3(64) + CHARACTER FILNAM*(*) + INTEGER EMBLFT + EXTERNAL EMBLFT + CALL FILLR(S2,64,0.0) + ISTRAN = 0 + IF(JSTRAN.EQ.1) ISTRAN = 1 +10 CONTINUE + NOBJ = 0 + IOK = EMBLFT(IDEVE,KBOUT,'CDS',OPRATR,ISTRAN, + +POSNS,MAXPOS,NPOS,NOBJ) + IF(IOK.NE.0) THEN + CLOSE(UNIT=IDEVE) + RETURN + END IF + IF(ISTRAN.EQ.0) THEN + K = NPOS + DO 100 I=1,NOBJ + NSEG = POSNS(K) + IB = 1 + IF((POSNS(K-NSEG).GE.J1).AND.(POSNS(K-1).LE.J2)) THEN + WRITE(KBOUT,*)'Data from end point ',POSNS(K-NSEG) + DO 50 J=K-NSEG,K-1,2 + L = POSNS(J+1) - POSNS(J) + 1 + CALL SQCOPY(SEQ(POSNS(J)),SEQW(IB),L) + IB = IB + L +C note not checking for within j1,j2 + 50 CONTINUE + IB = IB - 1 + IB = 3*(IB/3) + CALL FILLR(S1,64,0.0) + CALL CALCOD(S1,SEQW,IB) + CALL ADDR(S1,S2,64) + IF(NORM.EQ.1)THEN + CALL COPYR(S1,S3,64) + CALL NORMAA(S3,100.,PAA) + CALL WRTCOD(S3,IDEV,PAA) + ELSE + CALL WRTCOD(S1,IDEV,PAA) + END IF + IF(IDEV.EQ.KBOUT)THEN + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL BCOMPC(S1,IDEV) + CALL AACOMP(S1,IDEV,PAA) + IF(IDEV.EQ.KBOUT)THEN + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + END IF + END IF + K = K - NSEG - 1 + 100 CONTINUE + ELSE + K = NPOS + DO 200 I=1,NOBJ + NSEG = POSNS(K) + IB = 1 + IF((POSNS(K-NSEG).GE.J1).AND.(POSNS(K-1).LE.J2)) THEN + WRITE(KBOUT,*)'Data from end point ',POSNS(K-1) + DO 60 J=K-NSEG,K-1,2 + L = POSNS(J+1) - POSNS(J) + 1 + CALL SQCOPY(SEQ(POSNS(J)),SEQW(IB),L) + IB = IB + L + 60 CONTINUE + IB = IB - 1 + CALL SQREV(SEQW,IB) + CALL SQCOM2(SEQW,IB) + IB = 3*(IB/3) + CALL FILLR(S1,64,0.0) + CALL CALCOD(S1,SEQW,IB) + CALL ADDR(S1,S2,64) + IF(NORM.EQ.1)THEN + CALL COPYR(S1,S3,64) + CALL NORMAA(S3,100.,PAA) + CALL WRTCOD(S3,IDEV,PAA) + ELSE + CALL WRTCOD(S1,IDEV,PAA) + END IF + IF(IDEV.EQ.KBOUT)THEN + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL BCOMPC(S1,IDEV) + CALL AACOMP(S1,IDEV,PAA) + IF(IDEV.EQ.KBOUT)THEN + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + END IF + END IF + K = K - NSEG - 1 + 200 CONTINUE + END IF + IF((JSTRAN.EQ.2).AND.(ISTRAN.EQ.0)) THEN + ISTRAN = 1 + REWIND(IDEVE) + GO TO 10 + END IF + WRITE(IDEV,9991) +9991 FORMAT(5X,'Codon totals over all genes') + IF(NORM.EQ.1)THEN + CALL COPYR(S2,S3,64) + CALL NORMAA(S3,100.,PAA) + CALL WRTCOD(S3,IDEV,PAA) + ELSE + CALL WRTCOD(S2,IDEV,PAA) + END IF + IF(IDEV.EQ.KBOUT)THEN + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL BCOMPC(S2,IDEV) + CALL AACOMP(S2,IDEV,PAA) + END + SUBROUTINE CODONS(SEQ,IDIM,J1,J2,IDEV,IDEVE,FILNAM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PAA,POSNS,MAXPOS,SEQW) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),FILNAM*(*),PAA(5,5,5),SEQW(IDIM),OPRATR*20 + REAL S1(64),S2(64),S3(64) + INTEGER NORM,ANSE,POSNS(MAXPOS) + CALL SHOWFU(KBOUT, + +'Calculate base, codon and amino acid compositions') + CALL GETPAR(23,3,IOK,JSTRAN,NORM,ANSE, + +IPAR4,IPAR5, + +IPAR6,IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + JSTRAN = 0 + NORM = 0 + ANSE = 0 + CALL CODND1(JSTRAN,NORM,ANSE,IDEVE,FILNAM,OPRATR, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + IF(ANSE.EQ.1) THEN + CALL CODEMB(SEQ,IDIM,J1,J2,SEQW,PAA,JSTRAN,IDEVE,IDEV, + + POSNS,MAXPOS,KBIN,KBOUT,OPRATR,S1,S2,S3,NORM,FILNAM,IOK) + CLOSE(UNIT=IDEVE) + RETURN + END IF + CALL FILLR(S2,64,0.0) +100 CONTINUE + CALL FILLR(S1,64,0.0) + CALL TRAND8(IDIM,J1,J2,JSTRAN,I1,I2,'Count over', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + IF(I1.EQ.0) GO TO 200 + IDIM1 = I2-I1+1 + CALL CALCOD(S1,SEQ(I1),IDIM1) + IF(JSTRAN.EQ.1)CALL CODCOM(S1) + CALL ADDR(S1,S2,64) + IF(NORM.EQ.1)THEN + CALL COPYR(S1,S3,64) + CALL NORMAA(S3,100.,PAA) + CALL WRTCOD(S3,IDEV,PAA) + ELSE + CALL WRTCOD(S1,IDEV,PAA) + END IF + IF(IDEV.EQ.KBOUT)THEN + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL BCOMPC(S1,IDEV) + CALL AACOMP(S1,IDEV,PAA) + IF(IDEV.EQ.KBOUT)THEN + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + END IF + GO TO 100 +200 CONTINUE + WRITE(IDEV,9991) +9991 FORMAT(5X,'Codon totals over all genes') + IF(NORM.EQ.1)THEN + CALL COPYR(S2,S3,64) + CALL NORMAA(S3,100.,PAA) + CALL WRTCOD(S3,IDEV,PAA) + ELSE + CALL WRTCOD(S2,IDEV,PAA) + END IF + IF(IDEV.EQ.KBOUT)THEN + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + END IF + CALL BCOMPC(S2,IDEV) + CALL AACOMP(S2,IDEV,PAA) + END + SUBROUTINE CODIMP(SEQ,IDIM,FTABLE,IDFTAB,IDEVE,FILNAM, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,J1,J2,IDEV,POSNS,MAXPOS,SEQW) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + REAL FTABLE(IDFTAB) + CHARACTER SEQ(IDIM),FILNAM*(*),OPRATR*20,SEQW(IDIM) + INTEGER ANSE,POSNS(MAXPOS) + CALL FACTAB(FTABLE,99) + CALL SHOWFU(KBOUT,'Calculate codon improbability') + CALL GETPAR(28,2,IOK,JSTRAN,ANSE, + +IPAR3,IPAR4,IPAR5, + +IPAR6,IPAR7,IPAR8,IPAR9,IPAR10, + +IPAR11,IPAR12,IPAR13,IPAR14,IPAR15, + +IPAR16,IPAR17,IPAR18,IPAR19,IPAR20, + +IPAR21,IPAR22,IPAR23,IPAR24,IPAR25, + +IPAR26,IPAR27,IPAR28,IPAR29,IPAR30, + +IPAR31,IPAR32,IPAR33,IPAR34,IPAR35) + IF(IOK.NE.0) RETURN + CALL TRAND1(JSTRAN,ANSE,IDEVE,FILNAM, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,OPRATR,IOK) + IF(IOK.NE.0) THEN + IF(ANSE.EQ.1)CLOSE(UNIT=IDEVE) + RETURN + END IF + IF(ANSE.EQ.1) THEN + CALL CDIEMB(SEQ,IDIM,J1,J2,SEQW,JSTRAN,IDEVE,IDEV, + + POSNS,MAXPOS,KBOUT,FTABLE,IDFTAB,OPRATR,IOK) + CLOSE(UNIT=IDEVE) + RETURN + END IF +100 CONTINUE + CALL TRAND8(IDIM,J1,J2,JSTRAN,I1,I2,'Count over', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + IF(I1.EQ.0) RETURN + IDIM1 = I2-I1+1 + IF(JSTRAN.EQ.1) THEN + CALL SQCOPY(SEQ(I1),SEQW,IDIM1) + CALL SQREV(SEQW,IDIM1) + CALL SQCOM2(SEQW,IDIM1) + CALL CODMPP(SEQW,IDIM,FTABLE,IDFTAB,KBOUT,1,IDIM1,1,IDIM1, + + IDIM1,IDEV) + ELSE + CALL CODMPP(SEQ,IDIM,FTABLE,IDFTAB, + + KBOUT,1,IDIM,I1,I2,IDIM1,IDEV) + END IF + GO TO 100 + END + SUBROUTINE CDIEMB(SEQ,IDIM,J1,J2,SEQW,JSTRAN,IDEVE,IDEV, + +POSNS,MAXPOS,KBOUT,FTABLE,IDFTAB,OPRATR,IOK) +C codon improbability using ft + CHARACTER SEQ(IDIM),OPRATR*(*),SEQW(IDIM) + INTEGER POSNS(MAXPOS) + REAL FTABLE(IDFTAB) + INTEGER EMBLFT + EXTERNAL EMBLFT + ISTRAN = 0 + IF(JSTRAN.EQ.1) ISTRAN = 1 +10 CONTINUE + NOBJ = 0 + IOK = EMBLFT(IDEVE,KBOUT,'CDS',OPRATR,ISTRAN, + +POSNS,MAXPOS,NPOS,NOBJ) + IF(IOK.NE.0) THEN + CLOSE(UNIT=IDEVE) + RETURN + END IF + IF(ISTRAN.EQ.0) THEN + K = NPOS + DO 100 I=1,NOBJ + NSEG = POSNS(K) + IB = 1 + IF((POSNS(K-NSEG).GE.J1).AND.(POSNS(K-1).LE.J2)) THEN + WRITE(KBOUT,*)'Data from end point ',POSNS(K-NSEG) + DO 50 J=K-NSEG,K-1,2 + L = POSNS(J+1) - POSNS(J) + 1 + CALL SQCOPY(SEQ(POSNS(J)),SEQW(IB),L) + IB = IB + L +C note not checking for within j1,j2 + 50 CONTINUE + IB = IB - 1 + IB = 3*(IB/3) + CALL CODMPP(SEQW,IDIM,FTABLE,IDFTAB,KBOUT,1,IB,1,IB,IB,IDEV) + END IF + K = K - NSEG - 1 + 100 CONTINUE + ELSE + K = NPOS + DO 200 I=1,NOBJ + NSEG = POSNS(K) + IB = 1 + IF((POSNS(K-NSEG).GE.J1).AND.(POSNS(K-1).LE.J2)) THEN + WRITE(KBOUT,*)'Data from end point ',POSNS(K-1) + DO 60 J=K-NSEG,K-1,2 + L = POSNS(J+1) - POSNS(J) + 1 + CALL SQCOPY(SEQ(POSNS(J)),SEQW(IB),L) + IB = IB + L + 60 CONTINUE + IB = IB - 1 + CALL SQREV(SEQW,IB) + CALL SQCOM2(SEQW,IB) + IB = 3*(IB/3) + CALL CODMPP(SEQW,IDIM,FTABLE,IDFTAB,KBOUT,1,IB,1,IB,IB,IDEV) + END IF + K = K - NSEG - 1 + 200 CONTINUE + END IF + IF((JSTRAN.EQ.2).AND.(ISTRAN.EQ.0)) THEN + ISTRAN = 1 + REWIND(IDEVE) + GO TO 10 + END IF + END + SUBROUTINE PLTEMB(JSTRAN,IDEVE, + +POSNS,MAXPOS,KBOUT,KEYWRD,OPRATR,YF,BLIPB,BLIPT, + + XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C plot features + CHARACTER OPRATR*(*),KEYWRD*(*) + INTEGER POSNS(MAXPOS) + INTEGER EMBLFT + EXTERNAL EMBLFT + ISTRAN = 0 + IF(JSTRAN.EQ.1) ISTRAN = 1 +10 CONTINUE + NOBJ = 0 + IOK = EMBLFT(IDEVE,KBOUT,KEYWRD,OPRATR,ISTRAN, + +POSNS,MAXPOS,NPOS,NOBJ) + IF(IOK.NE.0) THEN + CLOSE(UNIT=IDEVE) + RETURN + END IF + IF(ISTRAN.EQ.0) THEN + K = NPOS + DO 100 I=1,NOBJ + NSEG = POSNS(K) + DO 50 J=K-NSEG,K-1,2 + CALL PLTBAR(REAL(POSNS(J)),REAL(POSNS(J+1)), + + YF,BLIPB,BLIPT, + + XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C note not checking for within j1,j2 + 50 CONTINUE + K = K - NSEG - 1 + 100 CONTINUE + ELSE + K = NPOS + DO 200 I=1,NOBJ + NSEG = POSNS(K) + DO 60 J=K-NSEG,K-1,2 + CALL PLTBAR(REAL(POSNS(J)),REAL(POSNS(J+1)), + + YF,BLIPB,BLIPT, + + XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + 60 CONTINUE + K = K - NSEG - 1 + 200 CONTINUE + END IF + IF((JSTRAN.EQ.2).AND.(ISTRAN.EQ.0)) THEN + ISTRAN = 1 + REWIND(IDEVE) + GO TO 10 + END IF + END +C PLOTMAP + SUBROUTINE PLTMAQ(IDEV,FILNAM,POSNS,MAXPOS, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IS,IE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + INTEGER POSNS(MAXPOS) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),OPRATR*20,KEYWRD*15 + PARAMETER (IBLIPH=128) + CALL SHOWFU(KBOUT, + +'Display a map using a feature table file') + XMAX=IE + XMIN=IS + YMIN=0. + YMAX=ISYMAX + IY = IBLIPH + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + +'Feature table file name', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN +10 CONTINUE + REWIND IDEV + LIN = 3 + CALL GTSTR('Feature code','CDS',KEYWRD,LIN, + +KBOUT,KBIN,INFLAG) + IF(LIN.EQ.0) KEYWRD(1:3) = 'CDS' + CALL CCASE(KEYWRD,1) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) GO TO 900 + IF(KEYWRD.EQ.' ') GO TO 900 + 20 CONTINUE + LIN = 3 + CALL GTSTR('Operator','all',OPRATR,LIN, + +KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) GO TO 900 + CALL CCASE(OPRATR,1) + IF((LIN.EQ.0).OR.(OPRATR(1:3).EQ.'ALL')) OPRATR(1:1) = ' ' + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 20 + END IF + JSTRAN = 1 + CALL GSTRND(JSTRAN,IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + JSTRAN = JSTRAN - 1 + IF(JSTRAN.LT.0) GO TO 900 + MININ = 0 + MAXIN = MARGT + CALL GETINT(MININ,MAXIN,IY, + +'level',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) GO TO 900 + IY = IVAL +C YBASE=IBASE + YF=IY + CALL CLEARV + CALL VECTOM +C NEED BARS AT ENDS OF FEATURES FROM BLIPB TO BLIPT + BLIPB=YF-IBLIPH/2 + BLIPT=YF+IBLIPH/2 + CALL PLTEMB(JSTRAN,IDEV, + +POSNS,MAXPOS,KBOUT,KEYWRD,OPRATR,YF,BLIPB,BLIPT, + + XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL VT100M + GO TO 10 +900 CONTINUE + CLOSE(UNIT=IDEV) + END + SUBROUTINE PLTBAR(POSNL,POSNR,YF,BLIPB,BLIPT, + +XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C plot a bar for a feature + CALL LINE(POSNL,POSNR,YF,YF,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(POSNL,POSNL,BLIPB,BLIPT,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(POSNR,POSNR,BLIPB,BLIPT,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END + SUBROUTINE MBPRIM(FRAMEC,IDIMS,J1,J2,PRIME) + INTEGER FRAMEC(IDIMS),PRIME,FROTAT(0:2,0:2) + SAVE FROTAT + DATA FROTAT/3,2,1,1,3,2,2,1,3/ +C AUTHOR RODGER STADEN +C multiplies array framec(i1-i2) by 2 or 3 +C frame rotation depends on frame and sequence length +C on - strand only works for complete codons + IF(PRIME.LT.0) THEN +C special case for ft input + DO 1 I = J2-2,J1,-3 + FRAMEC(I) = FRAMEC(I) * PRIME * (-1) +1 CONTINUE + RETURN + END IF + I1 = J1 + I2 = J2 + IF(J1.GT.J2) THEN + I2 = IDIMS + DO 5 I = I1,I2,3 + FRAMEC(I) = FRAMEC(I) * PRIME + 5 CONTINUE + LFRAME = MOD(IDIMS,3) + IFRAME = MOD(J1,3) + I1 = FROTAT(LFRAME,IFRAME) + I2 = J2 + DO 10 I = I1,I2,3 + FRAMEC(I) = FRAMEC(I) * PRIME + 10 CONTINUE + ELSE + DO 20 I = I1,I2,3 + FRAMEC(I) = FRAMEC(I) * PRIME + 20 CONTINUE + END IF + END + SUBROUTINE SQTREE(SEQ,SEQN,IDIM,WORDP,IDE,TREE,MAXTRE, + +LEVELS,MAXLEV,IDM,KSTART, + +IDEV,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER SEQ(IDIM),HELPF*(*) + INTEGER SEQN(IDIM),WORDP(IDE),TREE(MAXTRE) + INTEGER LEVELS(MAXLEV) + PARAMETER (MAXLEN = 6, + + MAXCON = 4*MAXLEN) + INTEGER IALPHA(5) + INTEGER CONSTS(0:MAXCON) +C INTEGER CONSTS(MAXLEN) +C PROGRAM TO REPRESENT A SEQUENCE AS A TREE +C THE FIRST LEVEL OF THE TREE IS THE SEQUENCE COMPOSITION +C THE SECOND LEVEL CONTAINS ALL THE TWO LETTER WORDS PRESENT +C AND SO ON. AS THE WORD LENGTH INCREASES THE NUMBER OF +C DIFFERENT WORDS PRESENT DECREASES, AS DOES THE NUMBER +C OF OCCURRENCES OF EACH. THE ROUTINE FOLLOWS WORDS UNTIL +C THEY ONLY OCCUR ONCE. +C IN ITS SIMPLEST FORM THE ALGORITHM STARTS WITH THE COMPOSITION +C AND FOLLOWS WORDS UNTIL THEY ONLY OCCUR ONCE. THE METHOD +C ENCODED BELOW STARTS WITH SOME MINIMUM WORD LENGTH +C USING THE HASHING METHOD OF THE SHOTGUN PROGRAMS. IT THEN +C CONVERTS TO THE DATA STRUCTURE FOR THE TREE METHOD. THE CURRENT +C DATA STRUCTURE IS AS FOLLOWS: FOR ANY WORD LENGTH THERE ARE +C NWORD DIFFERENT REPEATED WORDS, FOR EACH OF THOSE WORDS +C THERE ARE IWORD OCCURRENCES. THE TREE IS STORED IN AN ARRAY +C NAMED TREE. THE FIRST ELEMENT FOR EACH LENGTH OF WORD CONTAINS +C THE NUMBER OF DIFFERENT WORDS OF THAT LENGTH THAT ARE REPEATED, +C THE NEXT ELEMENT THE NUMBER OF OCCURRENCES OF THE FIRST WORD, THE +C NEXT ELEMENTS POINT TO THE STARTS OF THE INDIVIDUAL WORDS, UNTIL +C THE COUNT FOR THE NEXT REPEATED WORD. THE START POSITION IN THE +C TREE FOR EACH WORD LENGTH IS STORED IN ARRAY LEVEL. +C +C tree structure: +C +C word length = wordl +C number of different repeated words of length wordl = nword +C number of occurrences of each repeated word = iword +C positions of words in the sequence = pword +C +C for each length of word (level of the tree) store: +C +C nword(wordl(level)),iword(1),pword(iword(1),1),pword(iword(1),2) +C ,,, [iword(1) elements],iword(2),pword(iword(2),1) +C ,,, +C +C we also store in array levels a pointer into tree: levels(n) points to the +C start of info for words of length n +C + CALL CONNUM(SEQ,SEQN,IDIM) +C COUNT OCCURENCES OF ALPHABET + CALL FILLI(IALPHA,IDM,0) + DO 10 I=1,IDIM + IALPHA(SEQN(I))=IALPHA(SEQN(I))+1 +10 CONTINUE + NALPHA=0 + DO 11 I=1,IDM + IF(IALPHA(I).NE.0)NALPHA=NALPHA+1 +11 CONTINUE +C CALC EXPECTED LONGEST DIRECT REPEAT +C lN = 2LOGN/LOG(1/V) - [1+LOG(1-V)/LOGV) + 0.5772/LOGV] + LOG2/LOGV +C WHERE V=SUM PI, I=1,NALPHA AND N=IDIM + V=0. + DO 12 I=1,NALPHA + V1=REAL(IALPHA(I))/REAL(IDIM) + V=V+V1*V1 +12 CONTINUE + VLOG=LOG(V) + VLOGR=LOG(1.0/V) + VLOG1M=LOG(1.0-V) + REALLN=(2.*LOG(REAL(IDIM))/VLOGR)-(1+(VLOG1M/VLOG)+ + + 0.5772/VLOG)+LOG(2.)/VLOG + LENEXP = NINT(REALLN) + WRITE(KBOUT,1010)LENEXP + 1010 FORMAT(' Expected length of longest repeat',I6) + LENGTH = MIN(MAXLEN,LENEXP) + MN = 1 + MX = LENGTH + JLEVEL = MX + CALL GETINT(MN,MX,JLEVEL,'Minumim word length', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF (IOK.NE.0) RETURN + LENGTH = IVAL + CALL BUSY(KBOUT) + IF (LENGTH.EQ.1) THEN + CALL SQTRE1(SEQN,IDIM,TREE,MAXTRE,IALPHA,IDM,IENDT) + ELSE + CALL SETCN(CONSTS,LENGTH,IDM,MAXCON) + CALL ENCOND(SEQN,IDIM,WORDP,IDE,IDM,CONSTS,LENGTH,MAXCON) + CALL CONV(SEQN,IDIM,WORDP,IDE,TREE,MAXTRE,IENDT,LENGTH) + CALL CONNUM(SEQ,SEQN,IDIM) + END IF +C + LEVEL = LENGTH + LEVELS(LEVEL) = 1 + IENDT = IENDT + 1 +C +C +C +300 CONTINUE +C +C + LEVEL=LEVEL+1 + IF(LEVEL.GT.MAXLEV)GO TO 650 + LEVELS(LEVEL)=IENDT + IENDT=IENDT+1 + NODES=TREE(LEVELS(LEVEL-1)) + NBRAN=0 + ITREE=LEVELS(LEVEL-1)+1 + DO 400 I=1,NODES + NBRAN1=TREE(ITREE) + ITREE=ITREE+1 + CALL NEWBRA(TREE,MAXTRE,IALPHA,NALPHA,NBRAN1,ITREE, + + NBRAN2,SEQN,IDIM,IENDT,IOK) + IF(IOK.NE.0)GO TO 600 + NBRAN=NBRAN+NBRAN2 + ITREE=ITREE+NBRAN1 +400 CONTINUE + TREE(LEVELS(LEVEL))=NBRAN + IF(NBRAN.LT.2) THEN + IF(NBRAN.EQ.0) LEVEL = LEVEL - 1 + GO TO 700 + END IF + GO TO 300 +C +C Come here with problems +C +600 CONTINUE + CALL ERROM(KBOUT,'Not enough memory') + LEVEL = LEVEL - 1 + GO TO 700 +650 CONTINUE + CALL ERROM(KBOUT,'Maximum repeat length too long') + LEVEL = LEVEL - 1 +C +C Come here to show results +C +700 CONTINUE + WRITE(KBOUT,1006)4*(IENDT-1),LEVEL + 1006 FORMAT(' Memory used in bytes',I8,'. Length of longest repeat',I6) + MN = LENGTH + MX = LEVEL + JLEVEL = MX + CALL GETINT(MN,MX,JLEVEL, + +'Show repeat frequencies for words of at least length', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF (IOK.NE.0) RETURN + JLEVEL = IVAL + ITREE=LEVELS(JLEVEL) + DO 800 ILEVEL=JLEVEL,LEVEL + ITREE=LEVELS(ILEVEL) + WRITE(IDEV,1008)ILEVEL,TREE(ITREE) + 1008 FORMAT(' For length',I6, + + ' the number of different repeated words is',I6) +C find numbers of each word + INODES=TREE(ITREE) + ITREE=ITREE+1 + IWORD=TREE(ITREE) + DO 750 NODE=1,INODES + ITREE=ITREE+IWORD+1 + IWORD=TREE(ITREE) +750 CONTINUE +800 CONTINUE +C +C +801 CONTINUE +C +C + MN = LENGTH + MX = LEVEL + JLEVEL = MX + CALL GETINT(MN,MX,JLEVEL,'Show repeats for words of length', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF (IOK.NE.0) RETURN + JLEVEL = IVAL + MN = 2 + MX = 9999 + NOCCUR = MN + CALL GETINT(MN,MX,NOCCUR, + +'Show repeats for words occuring with frequency', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF (IOK.NE.0) RETURN + NOCCUR = IVAL +Ccccccccccccccccccccccccccccccccccccccccccccc + ITREE=LEVELS(JLEVEL) +C find numbers of different words + INODES=TREE(ITREE) + ITREE=ITREE+1 +C find number of occurences of first word + IWORD=TREE(ITREE) +C loop for each of the different words + DO 850 NODE=1,INODES + IF(IWORD.LT.NOCCUR)GO TO 876 +C note this last line used to go to 850 not 876 +C enough occurences so output + WRITE(IDEV,1014)(SEQ(K),K=TREE(ITREE+1)-(JLEVEL-1), + +TREE(ITREE+1)) +1014 FORMAT(' ',50A1) + JJ=ITREE + DO 875 J=1,IWORD + JJ=JJ+1 + WRITE(IDEV,1015)(TREE(JJ)-JLEVEL+1)+KSTART-1 +1015 FORMAT(' occurs at ',I6) +875 CONTINUE +876 CONTINUE +C move tree pointer on to next word + ITREE=ITREE+IWORD+1 +C point to next node + IWORD=TREE(ITREE) +850 CONTINUE +900 CONTINUE + GO TO 801 + END + SUBROUTINE NEWBRA(TREE,IDTREE,IALPHA,NALPHA,NBRAN1,ITREE, + +NBRAN2,SEQ,IDSEQ,IENDT,IOK) + INTEGER IALPHA(NALPHA),TREE(IDTREE) + INTEGER SEQ(IDSEQ) + DO 10 I=1,NALPHA + IALPHA(I)=0 +10 CONTINUE +C loop for all elements this node + DO 20 I=ITREE,ITREE+NBRAN1-1 +C WRITE(*,*)'I',I +C point to seq + K=TREE(I)+1 +C WRITE(*,*)'K',K + IF(K.LE.IDSEQ)IALPHA(SEQ(K))=IALPHA(SEQ(K))+1 +20 CONTINUE +C zero number of branches next level + NBRAN2=0 +C how many new branches? + DO 30 I=1,NALPHA + IF(IALPHA(I).GT.1)NBRAN2=NBRAN2+1 +30 CONTINUE + IF(NBRAN2.LT.1)RETURN +C +C now store the values + DO 50 ICHAR=1,NALPHA + IF(IALPHA(ICHAR).LT.2)GO TO 50 +C save thickness + IF(IENDT.GT.IDTREE)GO TO 100 + TREE(IENDT)=IALPHA(ICHAR) + IENDT=IENDT+1 +C now look thru sequence and save relevent pointers + DO 40 I=ITREE,ITREE+NBRAN1-1 + K=TREE(I)+1 + IF(K.GT.IDSEQ)GO TO 40 + IF(SEQ(K).NE.ICHAR)GO TO 40 +C save pointer + IF(IENDT.GT.IDTREE)GO TO 100 + TREE(IENDT)=K + IENDT=IENDT+1 +40 CONTINUE +50 CONTINUE + IOK = 0 + RETURN +100 CONTINUE + IOK = 1 + END + SUBROUTINE CONV(POSN,IDIM,WORDP,IDE,TREE,MAXTRE,IENDT,LENGTH) + INTEGER POSN(IDIM),WORDP(IDE),TREE(MAXTRE) + IENDT = 1 + NWORD = 0 + DO 20 I = 1,IDE + IF(WORDP(I).NE.0)THEN + IP = WORDP(I) + IF(POSN(IP).NE.0)THEN + IWORD = 1 + IENDT = IENDT + 1 + IT = IENDT + NWORD = NWORD + 1 + K = IP + IENDT = IENDT + 1 + TREE(IENDT) = K + LENGTH - 1 +10 CONTINUE + IF(POSN(K).NE.0)THEN + K = POSN(K) + IENDT =IENDT + 1 + TREE(IENDT) = K + LENGTH - 1 + IWORD = IWORD + 1 + GO TO 10 + END IF + TREE(IT) = IWORD + END IF + END IF +20 CONTINUE + TREE(1) = NWORD + END + SUBROUTINE SQTRE1(SEQN,IDIM,TREE,MAXTRE,IALPHA,IDM,IENDT) + INTEGER SEQN(IDIM),TREE(MAXTRE),IALPHA(IDM) + IENDT = 2 + TREE(1) = IDM - 1 + DO 200 I = 1,IDM - 1 + TREE(IENDT) = IALPHA(I) + IENDT = IENDT + 1 + DO 100 J = 1,IDIM + IF(SEQN(J).EQ.I) THEN + TREE(IENDT) = J + IENDT = IENDT + 1 + END IF + 100 CONTINUE + 200 CONTINUE + IENDT = IENDT - 1 + END diff --git a/src/staden/asubs89.f b/src/staden/asubs89.f new file mode 100644 index 0000000..d9cc01c --- /dev/null +++ b/src/staden/asubs89.f @@ -0,0 +1,1055 @@ +C ASUBS89 +C SUBROUTINES FOR ANALYSIS PROGRAMS ANALYSEQ AND ANALYSEP +C AUTHOR RODGER STADEN +C 11-1-90 GETNAM changed use of inflag=2 to set idnlst to 0 +C Added sqpf7, sepf7 sqpf5 and removed them from anals89, +C analps89 +C 6-7-90 Added showfu +C 5-11-90 Changed rdwmt to show/not show title, changed mkwt accordingly +C 7-11-90 Changed iopt in mkwt to fit with changes in analps89 and +C anals89 (means adding 1 to old values) +C added new routine gstrnd +C 18-4-91 added new weight matrix routines (initially for splice search) +C BUBDEL +C GETNAM +C PSRCHX +C BUBBLE +C PLTMAP +C IEMBL +C SEPFIT +C SPFIT +C GETSCR +C EDITSQ ETC + SUBROUTINE SQPF7(SEQNCE,IDIM1,STRING,IDIM2,MATCH, + +ITOT,ITOTEL,ITOTID, + +ITOTP,KSTART,J1,J2,ISS,PR,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + CHARACTER SEQNCE(IDIM1),STRING(IDIM2),MATCH(IDIM2),HELPF*(*) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + IOK = 1 + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + WRITE(KBOUT,1013)PR,ITOTP +1013 FORMAT(/,' Total scoring positions above',F7.3,' percent =',I4) + IF(ITOTP.GT.0) THEN + IF(ITOTP.GT.1)CALL BUBDEL(ITOT,ITOTEL,ITOTP) + WRITE(KBOUT,1002)(ITOT(K),K=1,MIN(10,ITOTP)) + WRITE(KBOUT,1006)(ITOTEL(K),K=1,MIN(10,ITOTP)) +1002 FORMAT( ' Scores ',10I7) +1006 FORMAT( ' Positions',10I7) + NSEE = 0 + CALL GETINT(0,ITOTP,NSEE,'Display',IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + NSEE = IVAL + IF(NSEE.GT.0)THEN + DO 300 I=1,NSEE + K=ITOTEL(I)-KSTART+1 + WRITE(IDEV,1008) +1008 FORMAT( ) + CALL SQMTCH( SEQNCE(K),STRING,MATCH,IDIM2) + CALL FMT4LN(SEQNCE(K),STRING,MATCH,IDIM2,K,ISS,IDEV) +300 CONTINUE + END IF + END IF + IOK = 0 + END + SUBROUTINE SEPF7(SEQNCE,IDIM1,STRING,IDIM2,MATCH, + +ITOT,ITOTEL,ITOTID, + +ITOTP,MINP,KSTART,J1,J2,ISS,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,IOK) + CHARACTER SEQNCE(IDIM1),STRING(IDIM2),MATCH(IDIM2),HELPF*(*) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + IOK = 1 + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN + WRITE(KBOUT,1013)MINP,ITOTP +1013 FORMAT(/,' For score',I6,' the number of matches=',I6) + IF(ITOTP.GT.0) THEN + IF(ITOTP.GT.1)CALL BUBDEL(ITOT,ITOTEL,ITOTP) + WRITE(KBOUT,1002)(ITOT(K),K=1,MIN(10,ITOTP)) + WRITE(KBOUT,1006)(ITOTEL(K),K=1,MIN(10,ITOTP)) +1002 FORMAT( ' Scores ',10I7) +1006 FORMAT( ' Positions',10I7/) + NSEE = 0 + CALL GETINT(0,ITOTP,NSEE,'Display',IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + NSEE = IVAL + IF(NSEE.GT.0)THEN + DO 300 I=1,NSEE + K=ITOTEL(I)-KSTART+1 + WRITE(IDEV,1008) +1008 FORMAT( ) + CALL SQMTCH( SEQNCE(K),STRING,MATCH,IDIM2) + CALL FMT4LN(SEQNCE(K),STRING,MATCH,IDIM2,K,ISS,IDEV) +300 CONTINUE + END IF + END IF + IOK = 0 + END + SUBROUTINE SQPF5(IDIM2,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,PR,MINP,IOK) + CHARACTER HELPF*(*) + REAL MININ,MAXIN + IOK = 1 + MININ = 1. + MAXIN = 100. + CALL GETRL(MININ,MAXIN,PR,'Percent match',VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + PR = VALUE + PRR = PR/100. + XIDIM2 = REAL(IDIM2) + XIDIM2 = XIDIM2 * PRR + MINP = NINT(XIDIM2) + IOK = 0 + END + SUBROUTINE GETRKB(SEQ,ID,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH, + +IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + CHARACTER SEQ(ID),A + IOK = 1 + WRITE(KBOUT,1004) +1004 FORMAT(' Define search strings by typing a string name', + +/,' followed by the string(s)') + JD = 1 +1 CONTINUE + L = 0 + CALL GETSTR('Name',A,SEQ(JD),20,L,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.3) GO TO 50 + JD = JD + L + SEQ(JD) = '/' + JD = JD + 1 +2 CONTINUE + L = 0 + CALL GETSTR('String(s)',A,SEQ(JD),75,L,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 2 + END IF + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.3) GO TO 50 +C IF(L.EQ.0) GO TO 2 + DO 40 I = JD, JD + L - 1 + IF(SEQ(I).EQ.' ') SEQ(I) = '/' +40 CONTINUE + JD = JD + L + SEQ(JD) = '/' + SEQ(JD+1) = '/' + JD = JD + 2 + GO TO 1 +50 CONTINUE + ID = JD - 1 + IOK = 0 + END + SUBROUTINE BUBDEL(LIST,LISTEL,IDIM) +C AUTHOR: RODGER STADEN + INTEGER LIST(IDIM),LISTEL(IDIM) + I=0 + J=0 +10 CONTINUE + IF(J.GT.I)I=J + I=I+1 + IF(I.EQ.IDIM)RETURN +20 CONTINUE + IF(LIST(I).GE.LIST(I+1))GO TO 10 + IF(J.LT.I)J=I + ITEMP=LIST(I) + LIST(I)=LIST(I+1) + LIST(I+1)=ITEMP + ITEMP=LISTEL(I) + LISTEL(I)=LISTEL(I+1) + LISTEL(I+1)=ITEMP + IF(I.GT.1)I=I-1 + GO TO 20 + END + SUBROUTINE GETNAM(NAMLST,IDNLST,NAMES,IDNAML,NAMEP,NAMLEN, + +MAXEN,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN +C 2-9-91 Added tupper to deal with lower case enzyme names +C routine to return namlst with numbers of wanted enzymes + CHARACTER NAMES(IDNAML),NAME*20,NEWNAM*20,HELPF*(*),TUPPER + INTEGER NAMLST(IDNLST),NAMEP(MAXEN),NAMLEN(MAXEN) + EXTERNAL TUPPER + NENZ=0 +10 CONTINUE + LENNAM = 0 + CALL GTSTR('Name',NAME,NEWNAM,LENNAM,KBOUT,KBIN,INFLAG) + IF(LENNAM.EQ.0) GO TO 50 + CALL CCASE(NEWNAM,1) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) THEN + IDNLST = 0 + RETURN + END IF + NAME = NEWNAM +C search for it in names + DO 20 I=1,MAXEN + IF(NAMLEN(I).NE.LENNAM)GO TO 20 +C name of correct length, do its chars match? + DO 19 J=1,LENNAM + IF(TUPPER(NAMES(NAMEP(I)+J-1)).NE.NAME(J:J))GO TO 20 +19 CONTINUE +C must match + NENZ=NENZ+1 + IF(NENZ.GT.IDNLST)THEN + WRITE(KBOUT,1003) +1003 FORMAT(' Too many names selected') + RETURN + END IF + NAMLST(NENZ)=I + GO TO 10 +20 CONTINUE +C no name match + WRITE(KBOUT,1004)NAME(1:LENNAM) +1004 FORMAT(' ',A,' not found in file') + GO TO 10 +50 CONTINUE + IDNLST=NENZ + END + SUBROUTINE PLSRCH(J1,J2, + +PSAVE,IFOUND,IBH,LEVEL,NAME,NAMLEN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C AUTHOR: RODGER STADEN + CHARACTER NAME*(*) + INTEGER PSAVE(IFOUND) + CALL VECTOM + XMAX=J2 + XMIN=J1 + YMAX=MARGT + YMIN=0. + Y=LEVEL + YP=Y+IBH + CALL TEXT(XMIN,Y,NAME,NAMLEN,0 + +,XMAX,XMIN,YMAX,YMIN,8,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX) + CALL LINE(XMIN,XMAX,Y,Y,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT, + +ISXMAX,ISYMAX) + DO 10 I=1,IFOUND + X=PSAVE(I) + CALL LINE(X,X,Y,YP,XMAX,XMIN,YMAX,YMIN,MARGL,MARGR,MARGB, + +MARGT,ISXMAX,ISYMAX) +10 CONTINUE +C now increase level ready for next entry + LEVEL=LEVEL+IBH + END +C PSRCHX + SUBROUTINE PSRCHX(LEVEL1,LEVEL,KBIN,KBOUT,IQUIT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CALL VT100M + CALL BPAUSE(KBIN,KBOUT,IQUIT) + IF(IQUIT.NE.0) RETURN + CALL YESNO(IQUIT,'Restart plotting from bottom of frame', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + CALL VECTOM + CALL CLEARV + IF(IQUIT.EQ.1)RETURN + CALL BPAUSE(KBIN,KBOUT,IQUIT) + IF(IQUIT.NE.0) RETURN + CALL CLEARG + LEVEL=LEVEL1 + END + SUBROUTINE BUBBLE(LIST,IDIM) +C AUTHOR: RODGER STADEN + INTEGER LIST(IDIM) + I=0 + J=0 +10 CONTINUE + IF(J.GT.I)I=J + I=I+1 + IF(I.EQ.IDIM)RETURN +20 CONTINUE + IF(LIST(I).LE.LIST(I+1))GO TO 10 + IF(J.LT.I)J=I + ITEMP=LIST(I) + LIST(I)=LIST(I+1) + LIST(I+1)=ITEMP + IF(I.GT.1)I=I-1 + GO TO 20 + END +C PLOTMAP + SUBROUTINE PLTMAP(IDEV,FILNAM,IDIM, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IS,IE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),FEATUR*8,STRAND,DESCRP(38) + CHARACTER ATOS*38 + EXTERNAL IEMBL + EXTERNAL ATOS + PARAMETER (IBLIPH=256) + CALL SHOWFU(KBOUT, + +'Display a map using an EMBL feature table file') + XMAX=IE + XMIN=IS + YMIN=0. + YMAX=ISYMAX + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + +'Map file name', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN +10 CONTINUE + REWIND IDEV + LIN = 3 + CALL GTSTR('Feature code','CDS',FEATUR,LIN, + +KBOUT,KBIN,INFLAG) + IF(LIN.EQ.0) FEATUR(1:3) = 'CDS' + CALL CCASE(FEATUR,1) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) GO TO 900 +1006 FORMAT(A) + IF(FEATUR.EQ.' ') GO TO 900 + ISTRND = 1 + CALL GSTRND(ISTRND,IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + ISTRND = ISTRND - 1 + IF(ISTRND.LT.0) GO TO 900 + STRAND=' ' + IF(ISTRND.EQ.1)STRAND='C' + IF(ISTRND.EQ.2)STRAND='B' +C7 WRITE(KBOUT,1001) +C1001 FORMAT(' ? SEQUENCE LEVEL IN DRAWING BOARD UNITS(DEF=0)=',$) +C READ(KBIN,1002,ERR=7)IBASE +C IF(IBASE.EQ.-99)THEN +C CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) +C GO TO 7 +C END IF +C IF(IBASE.LT.0)RETURN +C IF(IBASE.GT.MARGT)IBASE=0 + IY = IBLIPH + MININ = 0 + MAXIN = MARGT + CALL GETINT(MININ,MAXIN,IY, + +'level',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) GO TO 900 + IY = IVAL +C YBASE=IBASE + YF=IY + CALL CLEARV + CALL VECTOM +C NEED BARS AT ENDS OF FEATURES FROM BLIPB TO BLIPT + BLIPB=YF-IBLIPH/2 + BLIPT=YF+IBLIPH/2 +C FEATURE NAME PUT AT YT + YT=YF+IBLIPH/2 +C CALL LINE(XMIN,XMAX,YBASE,YBASE,XMAX,XMIN,YMAX,YMIN, +C +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +20 CONTINUE + LEMBL=IEMBL(IDEV,'FT',FEATUR,STRAND,IS,IE,IPOSNL,IPOSNR,0, + +DESCRP,KBOUT,JSTRAN) + IF(LEMBL.EQ.0)THEN + POSNL=IPOSNL + POSNR=IPOSNR + XMID=POSNL+(POSNR-POSNL)/2. + CALL LINE(POSNL,POSNR,YF,YF,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(POSNL,POSNL,BLIPB,BLIPT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(POSNR,POSNR,BLIPB,BLIPT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL TEXT(XMID,YT,ATOS(DESCRP,1),1, + + 0,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + GO TO 20 + END IF + CALL VT100M + GO TO 10 +900 CONTINUE + CLOSE(UNIT=IDEV) + END +C IEMBL + INTEGER FUNCTION IEMBL(IDEV,SCODE,FEATUR,STRAND,IS,IE, + +POSNL,POSNR,IWRITE,DESCR,KBOUT,JSTRAN) +C AUTHOR: RODGER STADEN + CHARACTER LINE(80),FEATUR*(*),STRAND,DESCR(38) + CHARACTER KEYNAM(8),LFTEND(6),RITEND(6),DIR(3),DESCRP(38) + CHARACTER*2 CODE,SCODE + INTEGER POSNL,POSNR,IWRITE + EQUIVALENCE (LINE(1),CODE) + EQUIVALENCE (LINE(6),KEYNAM) + EQUIVALENCE (LINE(15),LFTEND) + EQUIVALENCE (LINE(22),RITEND) + EQUIVALENCE (LINE(29),DIR) + EQUIVALENCE (LINE(35),DESCRP) +C +C READS UNTIL FINDS WANTED LINE TYPE (SCODE) +C OR END OF TEXT THIS SEQUENCE, OR END OF FILE +C RETURN CODES ARE 0,1,2 RESPECTIVELY +C WRITES ALL LINES, WANTED LINE, OR NONE CODE IWRITE=2,1,0 RESPECTIVELY +C JSTRAN IS A NUMBER DENOTING THE STRAND FOUND (0,1, (3=ERROR IN LIB)) +C SET FLAG FOR MORE DATA + IEMBL=0 +10 CONTINUE + READ(IDEV,1000,END=600)LINE +1000 FORMAT(80A1) + IF(IWRITE.EQ.2)WRITE(KBOUT,2000)LINE +2000 FORMAT(' ',80A1) + IF(CODE.EQ.'SQ')GO TO 550 + IF(CODE.NE.SCODE)GO TO 10 + IF(IWRITE.EQ.1)WRITE(KBOUT,2000)LINE + IF(SCODE.NE.'FT')RETURN +50 CONTINUE + DO 51 J=1,8 + IF(KEYNAM(J).NE.FEATUR(J:J))GO TO 10 +51 CONTINUE + IF(STRAND.NE.'B')THEN + IF(DIR(2).NE.STRAND)GO TO 10 + END IF +C SET FOUND STRAND + JSTRAN=3 + IF(DIR(2).EQ.'C')JSTRAN=1 + IF(DIR(2).EQ.' ')JSTRAN=0 + DO 52 J=1,38 + DESCR(J)=DESCRP(J) +52 CONTINUE +C GET POSITIONS +C <1 ? + IF(LFTEND(5).EQ.'<')THEN + POSNL=1 + ELSE + POSNL=IFROMC(LFTEND,6,KBOUT) + END IF +C MAY CONTAIN > SO NEED TO CHECK EACH POSITION! + DO 56 K=1,6 + KK=7-K + IF(RITEND(KK).EQ.'>')GO TO 57 +56 CONTINUE + KK=0 +57 CONTINUE +C MAY CONTAIN < ! + IF(RITEND(5).EQ.'<')THEN + POSNR=1 + ELSE +C THE > IS AT POSITION KK + POSNR=IFROMC(RITEND(KK+1),6-KK,KBOUT) + END IF +C COMPLEMENTARY STRAND? + IF(DIR(2).EQ.'C')THEN + I=POSNL + POSNL=POSNR + POSNR=I + END IF +C in range? + IF(POSNR.LT.IS)GO TO 10 + IF(POSNL.GT.IE)GO TO 10 + RETURN +550 CONTINUE +C SET FLAG FOR END OF THIS SEQUENCE'S HEADING + IEMBL=1 + RETURN +600 CONTINUE +C SET FLAG FOR END OF THIS LIBRARY + IEMBL=2 + END +C SQFIT + SUBROUTINE SPFIT(SEQ,IDIM1,STRING,IDIM2,ITOT,ITOTEL,ITOTID, + +IS,IE,MINP,ITOTP,SCORES,IDSCOR,KSTART) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER ITOT(ITOTID),ITOTEL(ITOTID) + INTEGER SCORES(IDSCOR,IDSCOR) + INTEGER DTONUM + EXTERNAL DTONUM +C + IDIF=(IE-IS+2)-IDIM2 +C IDIF IS THE NUMBER OF POSNS TO TRY +C IPSTR GOES FROM 1 TO IDIM2 IDIF TIMES + IPSEQ=IS-KSTART+1 + ITOTP=0 + DO 200 I=1,IDIF + NTOT=0 + IP=IPSEQ + DO 100 J=1,IDIM2 + NTOT=NTOT+SCORES(DTONUM(SEQ(IP)),DTONUM(STRING(J))) + IP=IP+1 +100 CONTINUE + IF(NTOT.GE.MINP)THEN + ITOTP=ITOTP+1 + IF(ITOTP.GT.ITOTID)RETURN + ITOT(ITOTP)=NTOT + ITOTEL(ITOTP)=IP-IDIM2+KSTART-1 + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + END + SUBROUTINE GETSCR(STRING,IDIM2,SCORES,IDSCOR,SMAX,SMIN) +C AUTHOR: RODGER STADEN + CHARACTER STRING(IDIM2) + INTEGER SCORES(IDSCOR,IDSCOR),SMAX,SMIN,DTONUM + EXTERNAL DTONUM + SMAX=0 + SMIN=IDIM2*SCORES(IDSCOR,IDSCOR) + DO 2 I=1,IDIM2 + SMAX=SMAX+SCORES(DTONUM(STRING(I)),DTONUM(STRING(I))) +2 CONTINUE + END + SUBROUTINE GETC(TOT,SUM,LINE,IDM,MAXLEN,IDEV,KBOUT,LENGTH, + +IOK) + INTEGER TOT(MAXLEN),SUM(IDM,MAXLEN) + CHARACTER LINE(MAXLEN) + INTEGER CTONUM + EXTERNAL CTONUM + IOK = 1 + DO 2 I=1,MAXLEN + TOT(I)=0 + DO 1 J=1,IDM + SUM(J,I)=0 +1 CONTINUE +2 CONTINUE + N=0 +10 CONTINUE +1003 FORMAT(1X,120A1) +1004 FORMAT(' ',I6,' ',120A1) + READ(IDEV,1003,END=100)LINE + N=N+1 + WRITE(KBOUT,1004)N,LINE + DO 20 I=1,MAXLEN + IF(LINE(I).EQ.' ')GO TO 10 + SUM(CTONUM(LINE(I)),I)=SUM(CTONUM(LINE(I)),I)+1 +20 CONTINUE + GO TO 10 +100 CONTINUE + IF(N.EQ.0)THEN + WRITE(KBOUT,*)' Empty file of aligned sequences' + RETURN + END IF +C NOW FIND LENGTH OF MOTIF + DO 40 I=1,MAXLEN + K=0 + L=I + DO 30 J=1,IDM + K=K+SUM(J,I) +30 CONTINUE + IF(K.EQ.0)GO TO 50 + TOT(I)=TOT(I)+K +40 CONTINUE +50 CONTINUE + LENGTH=L-1 + IOK = 0 + END + SUBROUTINE GETW(TOT,SUM,FREQ,LENGTH,MAXCHR,MAXLEN) + INTEGER TOT(LENGTH),SUM(MAXCHR,MAXLEN) + REAL FREQ(MAXCHR,MAXLEN) + DO 70 I=1,LENGTH + DO 60 J=1,MAXCHR + FREQ(J,I)=LOG((REAL(SUM(J,I)+1)/REAL(TOT(I)+MAXCHR))) +60 CONTINUE +70 CONTINUE + END + SUBROUTINE GETW2(SUM,FREQ,LENGTH,MAXCHR,MAXLEN) + INTEGER SUM(MAXCHR,MAXLEN) + REAL FREQ(MAXCHR,MAXLEN) + DO 70 I=1,LENGTH + DO 60 J=1,MAXCHR + FREQ(J,I)=REAL(SUM(J,I)) +60 CONTINUE +70 CONTINUE + END + SUBROUTINE WRTSCM(TITLE,LENGTH,MIDDLE,BOT,TOP,IDM, + +TOT,SUM,CHRSET,IDEV,MAXLEN) + INTEGER TOT(LENGTH),SUM(IDM,MAXLEN) + CHARACTER CHRSET(IDM),TITLE*(*) +C PROTEIN MATRICES DONT WRITE ROWS FOR -X? AND SPACE SO SET DIMENSION +C TO IDM-4 + MINUS = 1 + IF(IDM.EQ.26)MINUS = 4 + WRITE(IDEV,1018)TITLE +1018 FORMAT(' ',A) +1019 FORMAT(' P',20I4) +1020 FORMAT(' N',20I4) +1021 FORMAT(' ',A,20I4) +1022 FORMAT(' ',2I6,2F10.3) + WRITE(IDEV,1022)LENGTH,MIDDLE,BOT,TOP + NLINES=1+(LENGTH-1)/20 + K1=1 + DO 400 J=1,NLINES + K2=MIN((K1+19),LENGTH) + WRITE(IDEV,1019)(K,K=K1-MIDDLE,K2-MIDDLE) + WRITE(IDEV,1020)(TOT(K),K=K1,K2) + DO 390 I=1,IDM-MINUS + WRITE(IDEV,1021)CHRSET(I),(SUM(I,K),K=K1,K2) +390 CONTINUE + K1=K1+20 + IF(K1.GT.LENGTH)K1=LENGTH +400 CONTINUE + CLOSE(UNIT=IDEV) + END + SUBROUTINE RDWMTN(TOT,WT,MIDDLE, + +LENGTH,MAXLEN,YMIN,YMAX,IDEV,IFAIL,IDM,KBOUT,IPROB) +C AUTHOR: RODGER STADEN +C Same as rdwmt except for file closing + INTEGER WT(IDM,MAXLEN),TOT(MAXLEN) + CHARACTER LINE*79 +C PROTEIN MATRICES DONT READ ROWS FOR -X? AND SPACE SO SET DIMENSION +C TO IDM-4 + MINUS = 1 + IF(IDM.EQ.26)MINUS = 4 +C SET FAIL FLAG + IFAIL=1 +1000 FORMAT( ) +1001 FORMAT(2X,20I4) +1003 FORMAT(A) +1002 FORMAT(2X,2I6,2F10.3) +1004 FORMAT(' ',A) + DO 10 I = 1,MAXLEN + TOT(I) = 0 + DO 5 J = 1,IDM + WT(J,I) = 0 +5 CONTINUE +10 CONTINUE +C READ TITLE + READ(IDEV,1003,END=100,ERR=100)LINE + IF(IPROB.EQ.0) WRITE(KBOUT,1004)LINE +C READ PLOT VALUES ETC + READ(IDEV,1002,ERR=100,END=100) + +LENGTH,MIDDLE,YMIN,YMAX +C HOW MANY LINES TO READ? + NLINES=1+(LENGTH-1)/20 + K1=1 + DO 50 J=1,NLINES +C READ POSITION + READ(IDEV,1000,END=100,ERR=100) + K2=MIN((K1+19),LENGTH) +C READ TOTALS +C READ(IDEV,1001,ERR=100,END=100)(TOT(K),K=K1,K2) + READ(IDEV,1001,ERR=100,END=100) +C READ COUNTS + DO 25 I=1,IDM-MINUS + READ(IDEV,1001,ERR=100,END=100)(WT(I,K),K=K1,K2) +C ALLOW TOTALS IN FILE TO BE WRONG! + DO 24 K = K1,K2 + TOT(K) = TOT(K) + WT(I,K) +24 CONTINUE +25 CONTINUE + K1=K1+20 + IF(K1.GT.LENGTH)K1=LENGTH +50 CONTINUE +C SET FAIL FLAG TO GOOD + IFAIL=0 + RETURN +100 CONTINUE + END + SUBROUTINE GETWC(TOT,SUM,LENGTH,MAXCHR,MAXLEN, + +CEXACT,PEXACT,IEXACT) + INTEGER TOT(LENGTH),SUM(MAXCHR,MAXLEN) + INTEGER PEXACT(MAXLEN),CEXACT(MAXLEN) +C routine to find 100% conserved residues in wt matrices + IEXACT = 0 + DO 70 I=1,LENGTH + IF(TOT(I).NE.0) THEN + DO 60 J=1,MAXCHR + IF(TOT(I).EQ.SUM(J,I)) THEN + IEXACT = IEXACT + 1 + CEXACT(IEXACT) = J + PEXACT(IEXACT) = I + GO TO 61 + END IF +60 CONTINUE +61 CONTINUE + END IF +70 CONTINUE + END + INTEGER FUNCTION MATWTC(SEQ,IDSEQ,J1,J2,K1, + +CEXACT,PEXACT,IEXACT) +C find first position in seq where the chars (in index form) +C contained in cexact, and with relative positions pexact, is found +C else return end +1 + CHARACTER SEQ(IDSEQ) + INTEGER PEXACT(IEXACT),CEXACT(IEXACT) + INTEGER CTONUM + EXTERNAL CTONUM + MATWTC = J2 + 1 + DO 100 I=K1,J2-PEXACT(IEXACT)+1 + DO 50 J=1,IEXACT + IF(CTONUM(SEQ(I+PEXACT(J)-1)).NE.CEXACT(J)) GO TO 51 +50 CONTINUE + MATWTC = I + RETURN +51 CONTINUE +100 CONTINUE + END + SUBROUTINE RDWMT(TOT,WT,MIDDLE, + +LENGTH,MAXLEN,YMIN,YMAX,IDEV,IFAIL,IDM,KBOUT,IPROB) +C AUTHOR: RODGER STADEN + INTEGER WT(IDM,MAXLEN),TOT(MAXLEN) + CHARACTER LINE*79 +C PROTEIN MATRICES DONT READ ROWS FOR -X? AND SPACE SO SET DIMENSION +C TO IDM-4 + MINUS = 1 + IF(IDM.EQ.26)MINUS = 4 +C SET FAIL FLAG + IFAIL=1 +1000 FORMAT( ) +1001 FORMAT(2X,20I4) +1003 FORMAT(A) +1002 FORMAT(2X,2I6,2F10.3) +1004 FORMAT(' ',A) + DO 10 I = 1,MAXLEN + TOT(I) = 0 + DO 5 J = 1,IDM + WT(J,I) = 0 +5 CONTINUE +10 CONTINUE +C READ TITLE + READ(IDEV,1003,END=100,ERR=100)LINE + IF(IPROB.EQ.0) WRITE(KBOUT,1004)LINE +C READ PLOT VALUES ETC + READ(IDEV,1002,ERR=100,END=100) + +LENGTH,MIDDLE,YMIN,YMAX +C HOW MANY LINES TO READ? + NLINES=1+(LENGTH-1)/20 + K1=1 + DO 50 J=1,NLINES +C READ POSITION + READ(IDEV,1000,END=100,ERR=100) + K2=MIN((K1+19),LENGTH) +C READ TOTALS +C READ(IDEV,1001,ERR=100,END=100)(TOT(K),K=K1,K2) + READ(IDEV,1001,ERR=100,END=100) +C READ COUNTS + DO 25 I=1,IDM-MINUS + READ(IDEV,1001,ERR=100,END=100)(WT(I,K),K=K1,K2) +C ALLOW TOTALS IN FILE TO BE WRONG! + DO 24 K = K1,K2 + TOT(K) = TOT(K) + WT(I,K) +24 CONTINUE +25 CONTINUE + K1=K1+20 + IF(K1.GT.LENGTH)K1=LENGTH +50 CONTINUE + CLOSE(UNIT=IDEV) +C SET FAIL FLAG TO GOOD + IFAIL=0 + RETURN +100 CONTINUE + CLOSE(UNIT=IDEV) + END + SUBROUTINE MKWT(FREQ,SUM,TOT,CHRSET,IDM,MAXLEN, + +IDEV2,IDEV3,KBIN,KBOUT,LINE, + +FILNAM,IOPT,IHELPS,IHELPE,HELPF,IDEVH,IOK) +C AUTHOR RODGER STADEN + CHARACTER FILNAM*(*),HELPF*(*) + CHARACTER LINE(MAXLEN),TITLE*60,CHRSET(IDM) + INTEGER SUM(IDM,MAXLEN),TOT(MAXLEN) + REAL FREQ(IDM,MAXLEN) + IOK = 1 + IF(IOPT.EQ.3)THEN + CALL OPENF1(IDEV2,FILNAM,0,IOK,KBIN,KBOUT, + + 'Name of existing weight matrix file', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL RDWMT(TOT,SUM,MIDDLE,LENGTH,MAXLEN, + + CUTMIN,CUTMAX,IDEV2, + + IOK,IDM,KBOUT,0) + IF(IOK.NE.0) RETURN + END IF + CALL OPENF1(IDEV2,FILNAM,0,IOK,KBIN,KBOUT, + +'Name of aligned sequences file', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + IF(IOPT.EQ.2) THEN + CALL GETC(TOT,SUM,LINE,IDM,MAXLEN,IDEV2, + + KBOUT,LENGTH,IOK) + IF(IOK.NE.0)RETURN + END IF + WRITE(KBOUT,1006)LENGTH +1006 FORMAT(' Length of motif',I6) + IOK = 1 + CALL YESNO(IOPT,'Sum logs of weights', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOPT.LT.0) RETURN + CUTMIN = -10.0 + IF(IOPT.EQ.1)CUTMIN = 10. + CALL MASKW(SUM,LENGTH,IDM,MAXLEN,KBIN,KBOUT,TITLE, + +IOPT,IHELPS,IHELPE,HELPF,IDEVH) + IF(IOPT.LT.0) RETURN +C NOW CALC WEIGHTS + IF(CUTMIN.LT.0.0)CALL GETW(TOT,SUM,FREQ,LENGTH,IDM,MAXLEN) + IF(CUTMIN.GE.0.0)CALL GETW2(SUM,FREQ,LENGTH,IDM,MAXLEN) +C NOW APPLY THE WEIGHTS + REWIND IDEV2 + CALL APPLWT(FREQ,IDM,LENGTH,IDEV2,IDEV3,KBIN,KBOUT,LINE,MAXLEN, + +BOT,TOP,TITLE,MIDDLE,IOK) + CLOSE(UNIT=IDEV2) + IF(IOK.NE.0) RETURN + CALL OPENF1(IDEV2,FILNAM,1,IOK,KBIN,KBOUT, + +'Name for new weight matrix file', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL WRTSCM(TITLE,LENGTH,MIDDLE,BOT,TOP,IDM, + +TOT,SUM,CHRSET,IDEV2,MAXLEN) + CLOSE(UNIT=IDEV2) + RETURN + END + SUBROUTINE MASKW(SUM,LENGTH,IDM,MAXLEN,KBIN,KBOUT,MASK, + +IOPT,IHELPS,IHELPE,HELPF,IDEVH) + INTEGER SUM(IDM,MAXLEN) + CHARACTER MASK*(*),HELPF*(*) + CALL YESNO(IOPT,'Use all motif positions', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOPT.LT.0) RETURN +5 CONTINUE + IF(IOPT.EQ.1)THEN + WRITE(KBOUT,1002) +1002 FORMAT(' x means use, - means ignore',/, + + ' e.g. xx-x---x-x means use positions 1,2,4,8,10') + LIN = 0 + CALL GTSTR('Mask',' ',MASK,LIN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 5 + END IF + IF(INFLAG.EQ.2) RETURN + IF(LIN.EQ.0) RETURN + DO 70 I=1,LENGTH + IF(MASK(I:I).EQ.'-')THEN + DO 60 J=1,IDM + SUM(J,I) = 0 +60 CONTINUE + END IF +70 CONTINUE + END IF + END + SUBROUTINE APPLWT(FREQ,IDM,LENGTH,IDEV,IDEV3,KBIN,KBOUT, + +LINE,MAXLEN,BOT,TOP,TITLE,MIDDLE,IOK) + REAL FREQ(IDM,MAXLEN) + CHARACTER LINE(MAXLEN),TITLE*(*) + INTEGER CTONUM + EXTERNAL CTONUM + IOK = 1 + N=0 + TOP=-99999. + BOT=9999999. + WRITE(KBOUT,*)' Applying weights to input sequences' + SMEAN = 0. + SUMSQ = 0. +1003 FORMAT(1X,120A1) +1004 FORMAT(' ',I4,' ',F12.3,' ',120A1) +200 CONTINUE + READ(IDEV,1003,END=300)LINE + N=N+1 + SCORE=0. + DO 210 I=1,LENGTH + SCORE=SCORE+FREQ(CTONUM(LINE(I)),I) +210 CONTINUE + WRITE(IDEV3,1004)N,SCORE,(LINE(K),K=1,LENGTH) + IF(SCORE.GT.TOP)TOP=SCORE + IF(SCORE.LT.BOT)BOT=SCORE + SMEAN=SMEAN+SCORE + SUMSQ=SUMSQ+SCORE*SCORE + GO TO 200 +300 CONTINUE + IF(N.LT.1)THEN + WRITE(KBOUT,*)' Error: empty sequence file' + RETURN + END IF + SMEAN=SMEAN/N + SM=SMEAN + SMEAN=SMEAN*SMEAN + SUMSQ=SUMSQ/N + SD = 0. + T = SUMSQ - SMEAN + IF(T.GT.0.)SD = SQRT(T) + SMM3=SM-3*SD + SMP3=SM+3*SD + WRITE(KBOUT,1000)TOP,BOT +1000 FORMAT(' Top score',F12.3,' Bottom score',F12.3) + WRITE(KBOUT,1001)SM,SD +1001 FORMAT(' Mean',F12.3,' Standard deviation',F12.3) + WRITE(KBOUT,1002)SMM3,SMP3 +1002 FORMAT(' Mean minus 3.sd',F12.3,' Mean plus 3.sd',F12.3) + BOT=SMM3 + TOP=SMP3 + XMN = -999. + XMX = 9999. + CALL GETRL(XMN,XMX,BOT,'Cutoff score',VAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + BOT = VAL + XMN = BOT + XMX = 999. + CALL GETRL(XMN,XMX,TOP,'Top score for scaling plots', + +VAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + TOP = VAL + MN = 0 + MX = LENGTH + MIDDLE = 1 + CALL GETINT(MN,MX,MIDDLE,'Position to identify',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MIDDLE = IVAL +305 CONTINUE + LIN = 0 + CALL GTSTR('Title',' ',TITLE,LIN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 305 + END IF + IF(INFLAG.EQ.2) RETURN + IOK = 0 + END + SUBROUTINE GETWM(TOT,SUM,FREQ,LENGTH,MATRIX,MAXCHR,MAXLEN) + INTEGER TOT(LENGTH),SUM(MAXCHR,MAXLEN),MATRIX(MAXCHR,MAXCHR) + REAL FREQ(MAXCHR,MAXLEN) + DO 70 I=1,LENGTH + IPROD = 0 + DO 60 J=1,MAXCHR + DO 50 K = 1,MAXCHR + IPROD = IPROD + MATRIX(J,K) * (SUM(J,I)+1) +50 CONTINUE +60 CONTINUE + TOT(I) = IPROD +70 CONTINUE + DO 170 I=1,LENGTH + DO 160 J=1,MAXCHR + IPROD = 0 + DO 150 K = 1,MAXCHR + IPROD = IPROD + MATRIX(J,K) * (SUM(J,I)+1) +150 CONTINUE + FREQ(J,I)=LOG(REAL(IPROD)/REAL(TOT(I))) +160 CONTINUE +170 CONTINUE + END + SUBROUTINE GETW1(SUM,FREQ,LENGTH,MAXCHR,MAXLEN) + INTEGER SUM(MAXCHR,MAXLEN) + REAL FREQ(MAXCHR,MAXLEN) + DO 70 I=1,LENGTH + DO 60 J=1,MAXCHR + FREQ(J,I) = 0. + IF(SUM(J,I).GT.0)FREQ(J,I) = 1. +60 CONTINUE +70 CONTINUE + END + SUBROUTINE MARGC(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + +HELPS,HELPE,MAXOPT,HELPF,IDEVH,KBIN,KBOUT) +C AUTHOR RODGER STADEN + INTEGER MARGB(MAXOPT),MARGT(MAXOPT),HELPS,HELPE + CHARACTER HELPF*(*) + CALL SHOWFU(KBOUT,'Reset plot positions') + NOPT = 0 + CALL GETINT(0,MAXOPT,NOPT,'Number of option to reposition', + +IVAL,KBIN,KBOUT,HELPS,HELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF(IVAL.EQ.0) RETURN + NOPT = IVAL + CALL MARGC1(ISXMAX,ISYMAX,MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + +HELPS,HELPE,HELPF,IDEVH,KBIN,KBOUT) + RETURN + END + SUBROUTINE SQPF3(STRING,NEW,MAXSTR,LENGTH, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER STRING(MAXSTR),NEW(MAXSTR) + IOK = 1 +10 CONTINUE + LIN = LENGTH + CALL GETSTR('String',STRING,NEW,MAXSTR,LIN,KBOUT,KBIN,INFLAG) + IF((LIN.LT.1).AND.(LENGTH.LT.1)) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + IF(LIN.GT.0)THEN + CALL SQCOPY(NEW,STRING,LIN) + LENGTH = LIN + END IF + IOK = 0 + END + SUBROUTINE SQPF2(SEQ2,IDIM3,STRING,IDIM2I,IDIM2,I1,I2, + +KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ2(IDIM3),STRING(IDIM2I) + IOK = 1 + MININ = 1 + MAXIN = IDIM3 + WRITE(KBOUT,1000) +1000 FORMAT(' Define string ends') + CALL GETINT(MININ,MAXIN,I1,'Start',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF(IVAL.NE.I1) THEN + I2 = IVAL + 10 + END IF + I1 = IVAL + MININ = I1 + 1 + MAXIN = I1 + IDIM2I - 1 + CALL GETINT(MININ,MAXIN,I2,'End',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + I2 = IVAL + IDIM2 =I2 - I1 + 1 + CALL SQCOPY(SEQ2(I1),STRING,IDIM2) + WRITE(KBOUT,1001)(STRING(K),K=1,IDIM2) +1001 FORMAT(' string=',50A1) + IOK = 0 + END + SUBROUTINE SQPF1(SEQ2,IDIM3I,IDIM3, + +IDEV,FILNAM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQ2(IDIM3I),FILNAM*(*) + IDIM3 = IDIM3I + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + +'String file', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + IOK = 1 + CALL ARRFIL(IDEV,SEQ2,IDIM3,KBOUT) + CLOSE(UNIT=IDEV) + IF(IDIM3.LT.1)RETURN + IOK = 0 + END + SUBROUTINE SQPFD1(SEQ2,IDIM3I,IDIM3,ANSTY,IDEVIN,FILE2, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*),FILE2*(*) + CHARACTER SEQ2(IDIM3I) + INTEGER ANSTY,CHOICE + CHOICE = ANSTY + IOK = 1 + CALL YESONO(CHOICE,'Type in string','Extract string from file', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(CHOICE.LT.0) RETURN + ANSTY = CHOICE + IF(ANSTY.EQ.1)THEN + CALL SQPF1(SEQ2,IDIM3I,IDIM3, + + IDEVIN,FILE2,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + END IF + IOK = 0 + END + SUBROUTINE GSTRND(IVAL,IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + CHARACTER HELPF*(*) + PARAMETER (MAXPRM = 13) + CHARACTER PROMPT(3)*(MAXPRM) + PROMPT(1) = '+ strand only' + PROMPT(2) = '- strand only' + PROMPT(3) = 'Both strands' + CALL RADION('Select strands',PROMPT,3,IVAL, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + END diff --git a/src/staden/bsplit.f b/src/staden/bsplit.f new file mode 100644 index 0000000..2f327bf --- /dev/null +++ b/src/staden/bsplit.f @@ -0,0 +1,62 @@ + SUBROUTINE FMAIN() + CHARACTER SEQ1(10000),SEQ2(10000) + CHARACTER*40 FILNAM,HELPF*40 + INTEGER DEVNOS(2) + CALL UNITNO(KBIN,KBOUT,DEVNOS,2) + WRITE(KBOUT,*)' bsplit v2.2' + FILNAM = ' ' + CALL OPENF1(DEVNOS(1),FILNAM,0,IOK,KBIN,KBOUT, + +'Name of gelinb file', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)STOP + IDIM=10000 + CALL ARRFIL(DEVNOS(1),SEQ1,IDIM,KBOUT) + CLOSE(UNIT=1) + FILNAM = ' ' + CALL OPENF1(DEVNOS(2),FILNAM,1,IOK,KBIN,KBOUT, + +'Name for new file of filenames', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)STOP +C REMOVE UNWANTED CHARS note this is very dangerous and is only for +C bbc data that has spurious characters. When +C we move to mac only we should skip this + I2=0 + DO 100 I=1,IDIM + IF((LGE(SEQ1(I),'-')).AND.(LLE(SEQ1(I),'Z')) .OR. + + (LGE(SEQ1(I),'a')).AND.(LLE(SEQ1(I),'z'))) THEN + I2=I2+1 + SEQ2(I2)=SEQ1(I) + END IF +100 CONTINUE + IDIM2=I2 + IP1=1 + WRITE(KBOUT,1005)IDIM-IDIM2 +1005 FORMAT(' number of illegal characters removed',I6) +200 CONTINUE + CALL CHARCT(SEQ2,IDIM2,IP1,NCHAR) + IF(NCHAR.GT.0)THEN + FILNAM(1:40)=' ' + DO 210 I=1,NCHAR + FILNAM(I:I)=SEQ2(IP1+I-1) +210 CONTINUE + WRITE(KBOUT,1001)FILNAM +1001 FORMAT(' creating file ',A) + CALL OPENRS(DEVNOS(1),FILNAM,LRECL,IOK,1) + IF(IOK.NE.0)STOP + WRITE(DEVNOS(2),1004)FILNAM +1004 FORMAT(A) + END IF + IP1=IP1+NCHAR+1 + IF(IP1.LT.IDIM2)THEN + CALL CHARCT(SEQ2,IDIM2,IP1,NCHAR) + IF(NCHAR.GT.0)THEN + CALL FMTDK(DEVNOS(1),SEQ2(IP1),NCHAR) + END IF + WRITE(KBOUT,1002)NCHAR +1002 FORMAT(' length',I6) + IP1=IP1+NCHAR+1 + IF(IP1.LT.IDIM2)GO TO 200 + END IF + CLOSE(DEVNOS(2)) + CLOSE(DEVNOS(1)) + END diff --git a/src/staden/conEdit.f b/src/staden/conEdit.f new file mode 100644 index 0000000..7105aa7 --- /dev/null +++ b/src/staden/conEdit.f @@ -0,0 +1,34 @@ + SUBROUTINE CONEDT(KBIN,KBOUT, + +GELNOS,GELSTR,GELEND, + +MAXDB,GELNO,LINNO,MAXLIN,RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,NGELS,NCONTS,GEL,GEL2,MAXGEL,LINCON,PERCD,IDM, + +IHELPS,IHELPE,HELPF,IDEVH,IDEV,IDEV1,IDEV2,IDEV3, + +IDEVT,IDEVC,LINLEN,FILNAM,LLINO,IOK,IERR,TEMP1) +C AUTHOR: SIMON DEAR + INTEGER RELPG(MAXDB),LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER GEL(MAXGEL),GEL2(MAXGEL) + CHARACTER FILNAM*(*),HELPF*(*) + INTEGER GELNOS(MAXDB),GELSTR(MAXDB),GELEND(MAXDB) + INTEGER GELNO(MAXLIN,2),LINNO(MAXLIN,2) +C DUMMY ROUTINE FOR NON X VERSIONS + END + SUBROUTINE JOINED(KBIN,KBOUT, + +GELNOS,GELSTR,GELEND, + +MAXDB,GELNO,LINNO,MAXLIN,RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,NGELS,NCONTS,GEL,GEL2,MAXGEL,LINCON,PERCD,IDM, + +IHELPS,IHELPE,HELPF,IDEVH,IDEV,IDEV1,IDEV2,IDEV3, + +IDEVT,IDEVC,LINLEN,FILNAM,LLINO,IOK,IERR,TEMP1) +C AUTHOR: SIMON DEAR + INTEGER RELPG(MAXDB),LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER GEL(MAXGEL),GEL2(MAXGEL) + CHARACTER FILNAM*(*),HELPF*(*) + INTEGER GELNOS(MAXDB),GELSTR(MAXDB),GELEND(MAXDB) + INTEGER GELNO(MAXLIN,2),LINNO(MAXLIN,2) +C DUMMY ROUTINE FOR NON X VERSIONS + END + SUBROUTINE JXEDIT(IDEV1,IDEV2,IDEV3,IDEVT,IDEVC, + +RELPG,LNGTHG,LNBR,RNBR,MAXGEL, + +IDBSIZ,LR,NR,LL,NL, + +I,IE,J,IE2,P,NG,NC,IDM,IEXT,IOK) +C DUMMY ROUTINE FOR NON X VERSIONS + END diff --git a/src/staden/contigEditor.c b/src/staden/contigEditor.c new file mode 100644 index 0000000..bf0c642 --- /dev/null +++ b/src/staden/contigEditor.c @@ -0,0 +1,1244 @@ +/* + Title: contigEditor + + File: contigEditor.c + Purpose: C language entry point and initialisation functions + Last update: 5 April 1993 + +*/ + + +/* +*/ + +#define CHKPNT(W,S) \ + { XSync(XtDisplay(W),False); fprintf(stderr,"CHKPNT: %s\n",S); } + + +/* ---- Includes ---- */ + +#include /* IMPORT: freopen, stdout, stderr, fprintf */ +/*#include */ + +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include + +#include "Sheet.h" +#include "contigEditor.h" +#include "locks.h" +#include "main.h" +#include "select.h" +#include "tagUtils.h" +#include "edUtils.h" +#include "extend.h" +#include "undo.h" +#include "fort.h" + + + +/* ---- Static variables ---- */ +static Widget editorShellWid; /* main editor shell */ +static Widget mainFormWid; /* main editor form */ + +static Widget buttonsWid; +static Widget exitWid; /* Leave Editor button */ +static Widget undoWid; /* Undo last edit command button */ +static Widget supermanWid; /* switch for Superuser class of edit commands */ +static Widget revealWid; /* switch for revealing hidden cutoff information */ +#ifdef nana +static Widget saveWid; /* save command button */ +#endif +static Widget lockWid; /* lock button for when joining */ +#ifdef nana +static Widget joinWid; /* lock button for when joining */ +#endif +static Widget labelWid; /* label displaying contig name */ +static Widget insertWid; /* edit mode - insertion */ +static Widget replaceWid; /* edit mode - replacement */ +static Widget nextWid; /* search for next problem in consensus */ + +static Widget disagreeFormWid; /* for displaying disagreements when joining */ +static Widget dummyWid; +Widget disagreeWid; + +float pcCut; /* cut off for consensus calculation */ +int editorMode; +enum States {StateDown=0,StateUp} editorState = StateDown; +static int lockOffset; + +/* save/lock state */ +int_f save_state; + +SaveStruct saveState; + +DefColours defColours; + + + +/* ---- My translation tables ---- */ +static char translationTable[] = + "\ + Ctrl: XawPositionSimpleMenu(edMenu) XtMenuPopup(edMenu)\n\ + : StartHighlight() buttonDown()\n\ + : ExtendHighlight()\n\ + : MakeSelection()\n\ + (2): buttonDown() invokeTrace()\n\ + : buttonDown()\n\ + : ExtendHighlight()\n\ + : ExtendHighlight()\n\ + : ExtendHighlight()\n\ + MetaLeft: MetaLeft()\n\ + MetaRight: MetaRight()\n\ + Right: caretRight()\n\ + Left: caretLeft()\n\ + Down: caretDown()\n\ + Up: caretUp()\n\ + Delete: deleteKey()\n\ + : keyPress()"; +/* + : SelectTag()\n\ + MetaUp: MetaUp()\n\ + */ +static XtActionsRec actionTable[] = { + {"caretRight", caretRight}, + {"caretLeft", caretLeft}, + {"caretDown", caretDown}, + {"caretUp", caretUp}, + {"deleteKey", deleteKey}, + {"keyPress", keyPress}, + {"buttonDown", buttonDown}, + {"invokeTrace", invokeTrace}, + {"StartHighlight", start_highlight}, + {"ExtendHighlight", extend_highlight}, + {"MakeSelection", make_selection}, + {"SelectTag", select_tag}, + {"MetaLeft", meta_left}, + {"MetaRight", meta_right}, + {"MetaUp", meta_up}, +}; +XtTranslations parsedTTable; + + +static char translationTable2[] = + ": selectRead()"; + +static XtActionsRec actionTable2[] = { + {"selectRead", selectRead}, +}; +XtTranslations parsedTTable2; + + + + + + + + + +/* ---- Private Functions ---- */ +static int LeaveAllowed; + +static void save() +/* +** Save Changes +*/ +{ + EdStruct *xx = intToEdStruct(0); + + saveDB( + xx, + saveState.idevr, + saveState.idevw, + saveState.idevn, + saveState.relpg, + saveState.lngthg, + saveState.lnbr, + saveState.rnbr, + saveState.maxgel + ); +} + +static void join() +/* +** Join request made +*/ +{ + joinDB( + saveState.idevr, + saveState.idevw, + saveState.idevn, + saveState.relpg, + saveState.lngthg, + saveState.lnbr, + saveState.rnbr, + saveState.maxgel, + saveState.llinol, + saveState.lnconl, + saveState.llinor, + saveState.lnconr, + saveState.ngels, + saveState.nconts, + saveState.idbsiz + ); + +} + +static void warningYesCallback(Widget w, + XtPointer client_data, XtPointer call_data) +/* +** A yes response to the +** warning about data not saved +*/ +{ + Widget popupWid = (Widget) client_data; + + + if (editorMode == EDITMODE) + save(); + else + join(); + + LeaveAllowed = 1; + XtDestroyWidget(popupWid); +} + +static void warningNoCallback(Widget w, + XtPointer client_data, XtPointer call_data) +/* +** A No response to the +** warning about data not saved +*/ +{ Widget popupWid = (Widget) client_data; + + LeaveAllowed = 1; + XtDestroyWidget(popupWid); +} + + static void warningCancelCallback(Widget w, + XtPointer client_data, XtPointer call_data) +/* +** A Cancel response to the +** warning about data not saved +*/ +{ Widget popupWid = (Widget) client_data; + + LeaveAllowed = 0; + XtDestroyWidget(popupWid); +} + +static void createLabelsForBox(Widget parentWid, char *warning) +{ + Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNlabel, warning); nargs++; + (void) XtCreateManagedWidget("warnPrompt", labelWidgetClass, + parentWid, args, nargs); + +} + +static int warnNotSaved(Widget parentWid, String warning) +/* +** Warn that changes have been made but contig hasn't been saved. +*/ +{ Widget warningWid, warnFormWid, warnPromptBox; + Widget yesWid, noWid, cancelWid; + Arg args[10]; + int nargs; + Position x, y; + Dimension width, height; + + + + + /* + Position the upper left hand corner of the popup at the + center of the parent widget. + */ + nargs = 0; + XtSetArg(args[nargs], XtNwidth, &width); nargs++; + XtSetArg(args[nargs], XtNheight, &height); nargs++; + XtGetValues(parentWid, args, nargs); + XtTranslateCoords(parentWid, + (Position) 0, (Position) height, + &x, &y); + nargs = 0; + XtSetArg(args[nargs], XtNx, x); nargs++; + XtSetArg(args[nargs], XtNy, y); nargs++; + warningWid = XtCreatePopupShell("warning", transientShellWidgetClass, + parentWid, + args, nargs); + + /* Create the form */ + nargs = 0; + warnFormWid = XtCreateManagedWidget("warnForm", formWidgetClass, + warningWid, args, nargs); + + nargs = 0; + warnPromptBox = XtCreateManagedWidget("warnBox", boxWidgetClass, + warnFormWid, args, nargs); + + createLabelsForBox(warnPromptBox,warning); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, warnPromptBox); nargs++; + yesWid = XtCreateManagedWidget("Yes", commandWidgetClass, + warnFormWid, args, nargs); + XtAddCallback(yesWid, XtNcallback, warningYesCallback, (XtPointer) warningWid); + + nargs = 0; + XtSetArg(args[nargs], XtNfromHoriz, yesWid); nargs++; + XtSetArg(args[nargs], XtNfromVert, warnPromptBox); nargs++; + noWid = XtCreateManagedWidget("No", commandWidgetClass, + warnFormWid, args, nargs); + XtAddCallback(noWid, XtNcallback, warningNoCallback, (XtPointer) warningWid); + + + nargs = 0; + XtSetArg(args[nargs], XtNfromHoriz, noWid); nargs++; + XtSetArg(args[nargs], XtNfromVert, warnPromptBox); nargs++; + cancelWid = XtCreateManagedWidget("Cancel", commandWidgetClass, + warnFormWid, args, nargs); + XtAddCallback(cancelWid, XtNcallback, + warningCancelCallback, (XtPointer) warningWid); + + /* This is a modal dialogue */ + LeaveAllowed = -1; + XtPopup(warningWid, XtGrabNonexclusive); + + while (LeaveAllowed==-1) + { + XEvent event; + + XtAppNextEvent(XtWidgetToApplicationContext(parentWid), &event); + XtDispatchEvent(&event); + } + + + return LeaveAllowed; +} + + + +static void editNextCallback(Widget w, XtPointer client_data, XtPointer call_data) +/* +** Next Problem button has been pushed +*/ +{ + EdStruct *xx = intToEdStruct(0); + + if (editorState == StateDown) return; + + findNextProblem(xx); +} + + +static void editRevealCallback(Widget w, XtPointer client_data, XtPointer call_data) +/* +** Next Problem button has been pushed +*/ +{ + EdStruct *xx; + Arg args[10]; + int nargs; + Boolean state; + int i; + + if (editorState == StateDown) return; + + nargs = 0; + XtSetArg(args[nargs], XtNstate, &state); nargs++; + XtGetValues(w, args, nargs); + + for (i=0;ireveal_cutoffs=state; + redisplaySequences (xx,xx->namesWid, xx->sequencesWid, xx->displayPos, xx->displayWidth); + } +} + +static void editLockCallback(Widget w, XtPointer client_data, XtPointer call_data) +/* +** Editor has been locked +*/ +{ + EdStruct *xx0 = intToEdStruct(0); + EdStruct *xx1 = intToEdStruct(1); + + /* + int nargs; + Arg args[10]; + + nargs=0; + XtSetArg(args[nargs], XtNstate, &currMode); nargs++; + XtGetValues(lockWid, args, nargs); + + if (currMode) + */ + lockOffset = xx1->displayPos - xx0->displayPos; + + redisplaySequences (xx0,xx0->namesWid, xx0->sequencesWid, xx0->displayPos, xx0->displayWidth); + redisplaySequences (xx1,xx1->namesWid, xx1->sequencesWid, xx1->displayPos, xx1->displayWidth); +} + +static void editUndoCallback(Widget w, XtPointer client_data, XtPointer call_data) +/* +** Undo last command request made +*/ +{ + undoLastCommand(); +} + +static void editExitCallback(Widget w, XtPointer client_data, XtPointer call_data) +/* +** Request made to leave the editor +*/ +{ + EdStruct *xx = intToEdStruct(0); + + if (editorState == StateDown) return; + + switch (editorMode) { + case EDITMODE: + if (editsMade(xx)) { + if (warnNotSaved(w,"Save changes?")) + { + XtPopdown(editorShellWid); + editorState = StateDown; + semaphoreRelease(activeLock); + } + } else { + XtPopdown(editorShellWid); + editorState = StateDown; + semaphoreRelease(activeLock); + } + break; + case JOINMODE: { + int overlapLength, wingeCount; + char warning[200]; + + countDisagreements(&overlapLength, &wingeCount); + if (overlapLength<=0) { + sprintf(warning, + "Contigs do not overlap\nPerform Join?\n"); + } else { + sprintf(warning, + "Percentage Mismatch:\n %5.2f%%\nPerform Join?\n", + (float)(100*wingeCount)/(float)overlapLength); + } + + if (warnNotSaved(w,warning)) + { + XtPopdown(editorShellWid); + editorState = StateDown; + semaphoreRelease(activeLock); + } + break; + } + default: + XtPopdown(editorShellWid); + editorState = StateDown; + semaphoreRelease(activeLock); + } + /* + ** We must shut down the search window as well + */ + if (editorState == StateDown) { + destroySearchWindow(); + } +} + +static void sliderScrollCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +/* +** left or right mouse button has caused the +** slider to move +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(wid)); + int position = (int) call_data; + + float topOfThumb; + int nargs; + Arg args[10]; + + if (editorState == StateDown) return; + + nargs=0; + XtSetArg(args[nargs], XtNtopOfThumb, &topOfThumb); nargs++; + XtGetValues(wid, args, nargs); + + /* Move the thumb by one screenful in the appropriate direction */ + if (position>0) + incDisplayPos(xx,D_screen); + else + decDisplayPos(xx,D_screen); +} + +static void leftleftCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +/* +** Jump left a long way +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(XtParent(wid))); + if (editorState == StateDown) return; + + decDisplayPos (xx,D_halfScreen); +} + +static void leftCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +/* +** Jump left a short way +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(XtParent(wid))); + if (editorState == StateDown) return; + + decDisplayPos (xx,D_character); +} + +static void rightCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +/* +** Jump right a short way +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(XtParent(wid))); + if (editorState == StateDown) return; + + incDisplayPos (xx,D_character); +} + +static void rightrightCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +/* +** Jump right a long way +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(XtParent(wid))); + if (editorState == StateDown) return; + + incDisplayPos (xx,D_halfScreen); +} + + +static void sliderJumpCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +/* +** Middle mouse button used to reposition scroll bar +*/ +{ + float percent = *((float *) call_data); + EdStruct *xx = widgetToEdStruct(XtParent(wid)); + + if (editorState == StateDown) return; + + setDisplayPosPercent(xx,percent); +} + +static void getFontDetails(int *width, int *height) +/* +** Get the font width and height +** for the contig editor window +** ASSUME a single font is used!! +*/ +{ + Arg args[10]; + int nargs; + XFontStruct *font; + + nargs = 0; + XtSetArg(args[nargs], XtNfont, &font); nargs++; + XtGetValues(labelWid, args, nargs); + + *width = font->max_bounds.width; + *height = font->max_bounds.ascent + font->max_bounds.descent; +} + +static void setInitialStates(int reveal_state) +/* +** Set the initial states of togglewidgets +*/ +{ + Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNstate, False); nargs++; + XtSetValues(lockWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNstate, reveal_state); nargs++; + XtSetValues(revealWid, args, nargs); + +} + +static void tweakGeometry1() +/* +** Some geometry tweaking must be done before widgets are realised +*/ +{ + EdStruct *xx; + + xx = intToEdStruct(1); + if (editorMode==EDITMODE) { + XtManageChild(nextWid); + XtUnmanageChild(lockWid); +#ifdef nana + XtUnmanageChild(joinWid); +#endif + XtUnmanageChild(disagreeFormWid); + XtUnmanageChild(xx->edWid); + XawFormDoLayout(mainFormWid,True); + } else { + XtUnmanageChild(nextWid); + XtManageChild(lockWid); +#ifdef nana + XtManageChild(joinWid); +#endif + XtManageChild(disagreeFormWid); + XtManageChild(xx->edWid); + XawFormDoLayout(mainFormWid,True); + } + +} + +static void tweakGeometry2() +/* +** Some geometry tweaking must be done after widgets are realised +*/ +{ + int i; + Arg args[10]; + int nargs; + + EdStruct *xx; + + /* + ** scroll button wids + */ + for (i=0;ifontWidth*NAMELEN+10); nargs++; + XtSetValues(xx->scrollButtonsWid, args, nargs); + + /* + Dimension width; + xx = intToEdStruct(i); + nargs = 0; + XtSetArg(args[nargs], XtNwidth, &width); nargs++; + XtGetValues(xx->namesWid, args, nargs); + fprintf(stderr,"width.a = %d\n",(int)width); + nargs = 0; + XtSetArg(args[nargs], XtNwidth, width); nargs++; + XtSetValues(xx->scrollButtonsWid, args, nargs); + nargs = 0; + XtSetArg(args[nargs], XtNwidth, &width); nargs++; + XtGetValues(xx->sequencesWid, args, nargs); + fprintf(stderr,"width.b = %d\n",(int)width); + nargs = 0; + XtSetArg(args[nargs], XtNwidth, width); nargs++; + XtSetValues(xx->sliderWid, args, nargs); + */ + } + + +} + + +/* ---- Exported functions ---- */ + + +void jxedit_( + int_f *idevr, /* unit number for relationships */ + int_f *idevw, /* unit number for working versions of sequences */ + int_f *idevn, /* unit number for sequence names */ + int_f *idevt, /* unit number for tag information */ + int_f *idevc, /* unit number for comment file */ + int_f *relpg, /* relative positions of gels in sequences */ + int_f *lngthg, /* lengths of sequences */ + int_f *lnbr, /* left neighbours */ + int_f *rnbr, /* right neighbours */ + int_f *maxgel, /* maximum length of gel */ + int_f *idbsiz, /* size of database */ + int_f *lnconl, /* left contig to join */ + int_f *llinol, /* left-most gel in contig of left contig */ + int_f *lnconr, /* right contig for join */ + int_f *llinor, /* left-most gel in contig of right contig */ + int_f *igell, /* left gel specified on entry */ + int_f *igellpos,/* position in left-most gel */ + int_f *igelr, /* right gel specified on entry */ + int_f *igelrpos,/* position in left-most gel */ + int_f *perced, /* cutoff for consensus calculation */ + int_f *ngels, /* number of gels in database */ + int_f *nconts, /* number of contigs in database */ + int_f *idm, /* database type */ + int_f *rcstate, /* reveal cutoff state */ + int_f *iok /* returns 0-saved (1) | 1-saved (2) | joined (4) */ + ) +/* +** Interface to FORTRAN for Join editor +*/ +{ + EdStruct *xx[2]; + int reveal_state; + int i; + + semaphoreGrab(activeLock); + editorState = StateUp; + editorMode = JOINMODE; + save_state = (int_f)0; + + reveal_state = (*rcstate != 0); + setInitialStates(reveal_state); + + /* + ** Save arguments for later use + */ + saveState.idevr = idevr; + saveState.idevw = idevw; + saveState.idevn = idevn; + saveState.idevt = idevt; + saveState.idevc = idevc; + saveState.relpg = relpg; + saveState.lngthg = lngthg; + saveState.lnbr = lnbr; + saveState.rnbr = rnbr; + saveState.maxgel = maxgel; + saveState.idbsiz = idbsiz; + saveState.llinol = llinol; + saveState.lnconl = lnconl; + saveState.llinor = llinor; + saveState.lnconr = lnconr; + saveState.perced = perced; + saveState.ngels = ngels; + saveState.nconts = nconts; + saveState.idm = idm; + pcCut = * (float *) perced; + + for (i=0; i<2; i++) { + int_f *llino = (i==0)?llinol:llinor; + + /* + ** Set up data structures + */ + xx[i] = intToEdStruct(i); + xx[i]->reveal_cutoffs = reveal_state; + if (initialiseDB(xx[i],idevr,idevw,idevn,relpg,lngthg,lnbr,rnbr,maxgel,idbsiz,llino)) { + if (i==1) freeDB(xx[0]); + return; + } + + /* + ** Set up display + */ + if (createEdDisplay(xx[i], + xx[i]->namesWid, + xx[i]->sequencesWid, + (i)?*igelr:*igell, + (i)?*igelrpos:*igellpos)) { + freeDB(xx[i]); + if (i==1) freeDB(xx[0]); + return; + } + + } + + tweakGeometry1(); + XtPopup(editorShellWid, XtGrabNone); + tweakGeometry2(); + + while (editorState != StateDown) + { + XEvent event; + + XtAppNextEvent(XtWidgetToApplicationContext(xx[0]->edWid), &event); + XtDispatchEvent(&event); + } + + for (i=0;i<2;i++) { + disown_selection(xx[i]); + freeDB(xx[i]); + cleanUpAllStacks(); + } + + *iok = save_state; +} + +void cxedit_( + int_f *idevr, /* unit number for relationships */ + int_f *idevw, /* unit number for working versions of sequences */ + int_f *idevn, /* unit number for sequence names */ + int_f *idevt, /* unit number for tag information */ + int_f *idevc, /* unit number for comment file */ + int_f *relpg, /* relative positions of gels in sequences */ + int_f *lngthg, /* lengths of sequences */ + int_f *lnbr, /* left neighbours */ + int_f *rnbr, /* right neighbours */ + int_f *maxgel, /* maximum length of gel */ + int_f *idbsiz, /* size of database */ + int_f *lincon, /* current contig to edit */ + int_f *llino, /* left-most gel in contig */ + int_f *igelno, /* gel number specified on entry */ + int_f *igelpos, /* position in gel */ + int_f *perced, /* cutoff for consensus calculation */ + int_f *idm, /* database type */ + int_f *rcstate, /* reveal cutoff state */ + int_f *iok /* returns 0-saved (1) | 1-saved (2) | joined (4) */ + ) +/* +** Interface to FORTRAN for Contig Editor +*/ +{ + + int reveal_state; + + EdStruct *xx = intToEdStruct(0); + semaphoreGrab(activeLock); + editorState = StateUp; + editorMode = EDITMODE; + save_state = (int_f)0; + + reveal_state = (*rcstate != 0); + setInitialStates(reveal_state); + + /* + ** Save arguments for later use + */ + saveState.idevr = idevr; + saveState.idevw = idevw; + saveState.idevn = idevn; + saveState.idevt = idevt; + saveState.idevc = idevc; + saveState.relpg = relpg; + saveState.lngthg = lngthg; + saveState.lnbr = lnbr; + saveState.rnbr = rnbr; + saveState.maxgel = maxgel; + saveState.idbsiz = idbsiz; + saveState.lincon = lincon; + saveState.llino = llino; + saveState.perced = perced; + saveState.idm = idm; + + /* + ** Set up data structures + */ + xx->reveal_cutoffs = reveal_state; + if (initialiseDB(xx,idevr,idevw,idevn,relpg,lngthg,lnbr,rnbr,maxgel,idbsiz,llino)) + return; + + /* + ** Set up display + */ + pcCut = * (float *) perced; + if (createEdDisplay(xx,xx->namesWid,xx->sequencesWid,*igelno,*igelpos)) { + freeDB(xx); + return; + } + + tweakGeometry1(); + XtPopup(editorShellWid, XtGrabNone); + tweakGeometry2(); + + while (editorState != StateDown) + { + XEvent event; + + XtAppNextEvent(XtWidgetToApplicationContext(xx->namesWid), &event); + XtDispatchEvent(&event); + } + + disown_selection(xx); + freeDB(xx); + cleanUpAllStacks(); + + *iok = save_state; +} + +Widget createEditWid(Widget parentWid, Widget fromVertWid, int closeToVert) +/* +** Create an form with all the trappings of an editor window +*/ +{ + EdStruct *xx; + Widget llWid, lWid, rWid, rrWid; + Arg args[10]; + int nargs; + + xx = getFreeEdStruct(); + if (xx == NULL) return NULL; + + getFontDetails(&xx->fontWidth,&xx->fontHeight); + + /* + ** create main form + */ + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + if (closeToVert) { + XtSetArg(args[nargs], XtNvertDistance, 0); nargs++; + } + xx->edWid = XtCreateManagedWidget("edForm", formWidgetClass, + parentWid, args, nargs); + + /* + ** Scrollers + */ + nargs = 0; + xx->scrollButtonsWid = XtCreateManagedWidget("scrollButtons", formWidgetClass, + xx->edWid, args, nargs); + nargs = 0; + llWid = XtCreateManagedWidget("leftleft", commandWidgetClass, + xx->scrollButtonsWid, args, nargs); + XtAddCallback(llWid, XtNcallback, leftleftCallback, NULL ); + nargs = 0; + XtSetArg(args[nargs], XtNfromHoriz, llWid); nargs++; + lWid = XtCreateManagedWidget("left", commandWidgetClass, + xx->scrollButtonsWid, args, nargs); + XtAddCallback(lWid, XtNcallback, leftCallback, NULL ); + nargs = 0; + XtSetArg(args[nargs], XtNfromHoriz, lWid); nargs++; + rWid = XtCreateManagedWidget("right", commandWidgetClass, + xx->scrollButtonsWid, args, nargs); + XtAddCallback(rWid, XtNcallback, rightCallback, NULL ); + nargs = 0; + XtSetArg(args[nargs], XtNfromHoriz, rWid); nargs++; + rrWid = XtCreateManagedWidget("rightright", commandWidgetClass, + xx->scrollButtonsWid, args, nargs); + XtAddCallback(rrWid, XtNcallback, rightrightCallback, NULL ); + + + + /* + ** A Slider + */ + nargs = 0; + XtSetArg(args[nargs], XtNfromHoriz, xx->scrollButtonsWid); nargs++; + XtSetArg(args[nargs], XtNhorizDistance, 0); nargs++; + XtSetArg(args[nargs], XtNorientation, XtorientHorizontal); nargs++; + XtSetArg(args[nargs], XtNheight, xx->fontHeight+4); nargs++; + XtSetArg(args[nargs], XtNwidth, xx->fontWidth*(xx->displayWidth)+8); nargs++; + + xx->sliderWid = XtCreateManagedWidget("slider", scrollbarWidgetClass, + xx->edWid, args, nargs); + XtAddCallback(xx->sliderWid, XtNjumpProc, sliderJumpCallback, NULL); + XtAddCallback(xx->sliderWid, XtNscrollProc, sliderScrollCallback, NULL); + + + + + /* + ** ... and a text widget or two or three + */ + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, xx->sliderWid); nargs++; + XtSetArg(args[nargs], XtNvertDistance, 0); nargs++; + XtSetArg(args[nargs], XtNcolumns, NAMELEN); nargs++; + xx->namesWid = XtCreateManagedWidget("names", sheetWidgetClass, + xx->edWid, args, nargs); + XtUninstallTranslations(xx->namesWid); + /* Add string to function bindings for our application actions */ + XtAppAddActions(XtWidgetToApplicationContext(xx->edWid), + actionTable2, XtNumber(actionTable2)); + parsedTTable2 = XtParseTranslationTable(translationTable2); + XtUninstallTranslations(xx->namesWid); + XtAugmentTranslations(xx->namesWid,parsedTTable2); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, xx->sliderWid); nargs++; + XtSetArg(args[nargs], XtNvertDistance, 0); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, xx->namesWid); nargs++; + XtSetArg(args[nargs], XtNhorizDistance, 0); nargs++; + XtSetArg(args[nargs], XtNcolumns, DEFAULT_DISPLAY_WIDTH); nargs++; + xx->sequencesWid = XtCreateManagedWidget("sequences", sheetWidgetClass, + xx->edWid, args, nargs); + + /* Add string to function bindings for our application actions */ + XtAppAddActions(XtWidgetToApplicationContext(xx->edWid), + actionTable, XtNumber(actionTable)); + parsedTTable = XtParseTranslationTable(translationTable); + XtUninstallTranslations(xx->sequencesWid); + + XtAugmentTranslations(xx->sequencesWid,parsedTTable); + + /* + ** Pop-up menu + */ + createEdMenu(xx->sequencesWid); + + return xx->edWid; +} + +Widget createDisagreeWid(Widget parentWid, Widget fromVertWid, int closeToVert) +/* +** Create a form widget with all the trappings of a +** disagreement window +*/ +{ + Arg args[10]; + int nargs; + int fontWidth, fontHeight; + + getFontDetails(&fontWidth, &fontHeight); + + /* + ** create main form + */ + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + if (closeToVert) { + XtSetArg(args[nargs], XtNvertDistance, 0); nargs++; + } + disagreeFormWid = XtCreateManagedWidget("disagreeForm", formWidgetClass, + parentWid, args, nargs); + nargs = 0; + XtSetArg(args[nargs], XtNrows, 1); nargs++; + XtSetArg(args[nargs], XtNcolumns, NAMELEN); nargs++; + dummyWid = XtCreateManagedWidget("dummy", sheetWidgetClass, + disagreeFormWid, args, nargs); + nargs = 0; + XtSetArg(args[nargs], XtNrows, 1); nargs++; + XtSetArg(args[nargs], XtNcolumns, DEFAULT_DISPLAY_WIDTH); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, dummyWid); nargs++; + disagreeWid = XtCreateManagedWidget("disagreements", sheetWidgetClass, + disagreeFormWid, args, nargs); + + XtUninstallTranslations(dummyWid); + XtUninstallTranslations(disagreeWid); + + return disagreeFormWid; +} + + +Widget CreateEditorShell(Widget parentWid) +/* +** Create all components of the +** Contig Editor +** and +** Join Editor +*/ +{ + Arg args[10]; + int nargs; + Widget ed1Wid; + Widget ed2Wid; + Widget dw; + + /* + ** Create the contig editor shell + */ + editorShellWid = XtCreatePopupShell ("Editor", + topLevelShellWidgetClass, + parentWid, + NULL, (Cardinal) 0); + + mainFormWid = XtCreateManagedWidget("mainForm", formWidgetClass, + editorShellWid, + NULL, (Cardinal) 0); + + + /* + ** The form widget holds a label + */ + nargs = 0; + XtSetArg(args[nargs], XtNfromHoriz, exitWid); nargs++; + labelWid = XtCreateManagedWidget("title", labelWidgetClass, + mainFormWid, args, nargs); + + + /* + ** Button box for contig/join editor + */ + nargs = 0; + XtSetArg(args[nargs], XtNfromHoriz, labelWid); nargs++; + buttonsWid = XtCreateManagedWidget("buttons", boxWidgetClass, + mainFormWid, args, nargs); + + /* + ** Toggle widgets for insert/replace edit modes + */ + nargs = 0; + insertWid = XtCreateManagedWidget("insert", toggleWidgetClass, + buttonsWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNradioGroup, insertWid); nargs++; + replaceWid = XtCreateManagedWidget("replace", toggleWidgetClass, + buttonsWid, args, nargs); + + /* + ** Superman toggle widget (for super-duper editing) + */ + nargs = 0; + supermanWid = XtCreateManagedWidget("superman", toggleWidgetClass, + buttonsWid, args, nargs); + + /* + ** Reveal cutoffs + */ + nargs = 0; + revealWid = XtCreateManagedWidget("reveal", toggleWidgetClass, + buttonsWid, args, nargs); + XtAddCallback(revealWid, XtNcallback, editRevealCallback, NULL ); + + /* + ** Undo command button + */ + nargs = 0; + undoWid = XtCreateManagedWidget("undo", commandWidgetClass, + buttonsWid, args, nargs); + XtAddCallback(undoWid, XtNcallback, editUndoCallback, NULL ); + + /* + ** Next (problem) command button + */ + nargs = 0; + nextWid = XtCreateManagedWidget("next", commandWidgetClass, + buttonsWid, args, nargs); + XtAddCallback(nextWid, XtNcallback, editNextCallback, NULL ); + +#ifdef nana + /* + ** Save command button + */ + nargs = 0; + saveWid = XtCreateManagedWidget("save", commandWidgetClass, + buttonsWid, args, nargs); + XtAddCallback(saveWid, XtNcallback, editSaveCallback, NULL ); +#endif + + /* + ** Lock command button + */ + nargs = 0; + lockWid = XtCreateManagedWidget("lock", toggleWidgetClass, + buttonsWid, args, nargs); + XtAddCallback(lockWid, XtNcallback, editLockCallback, NULL ); + +#ifdef nana + /* + ** Join command button + */ + nargs = 0; + joinWid = XtCreateManagedWidget("join", commandWidgetClass, + buttonsWid, args, nargs); + XtAddCallback(joinWid, XtNcallback, editJoinCallback, NULL ); +#endif + + /* + ** and an exit button + */ + nargs = 0; + exitWid = XtCreateManagedWidget("exit", commandWidgetClass, + buttonsWid, args, nargs); + XtAddCallback(exitWid, XtNcallback, editExitCallback, NULL ); + + /* + ** create editor + */ + ed1Wid = createEditWid(mainFormWid,buttonsWid,0); + dw = createDisagreeWid(mainFormWid,ed1Wid,1); + ed2Wid = createEditWid(mainFormWid,dw,1); + + /* + ** Create tag editor + */ + (void) createTagEditor(parentWid); + + + /* + ** Create search diologue + */ + (void) createSearchWidget(parentWid); + + return editorShellWid; + +} + + +int editModeIsInsert() +/* +** Find out if editor is in insert mode rather than replace mode +*/ +{ + String currMode; + + currMode = (String) XawToggleGetCurrent(insertWid); + if (currMode == NULL) + return 0; + else + return (strcmp (currMode,"insert") == 0); +} + +int editModeIsSuperman() +/* +** Find out if we are entitled to use privilaged edit commands +*/ +{ + Boolean currMode; + int nargs; + Arg args[10]; + + nargs=0; + XtSetArg(args[nargs], XtNstate, &currMode); nargs++; + XtGetValues(supermanWid, args, nargs); + + return currMode; +} + +int inJoinMode() +/* +** Find out if the editor is in join mode +*/ +{ + return editorMode==JOINMODE; +} + +int editorLocked() +/* +** Find out if the editor is locked (and in join mode of course) +*/ +{ + Boolean currMode; + int nargs; + Arg args[10]; + + if (inJoinMode()) { + nargs=0; + XtSetArg(args[nargs], XtNstate, &currMode); nargs++; + XtGetValues(lockWid, args, nargs); + + return (currMode); + } else + return 0; +} + +int editorLockedPos(int force) +/* +** Find out the locked position +*/ +{ + if (force) { + EdStruct *xx0 = intToEdStruct(0); + EdStruct *xx1 = intToEdStruct(1); + + return xx1->displayPos - xx0->displayPos; + } else + return (lockOffset); +} diff --git a/src/staden/contigEditor.h b/src/staden/contigEditor.h new file mode 100644 index 0000000..77b5668 --- /dev/null +++ b/src/staden/contigEditor.h @@ -0,0 +1,47 @@ +#ifndef _contigEditor_h +#define _contigEditor_h +#include +#include "fort.h" +extern Widget disagreeWid; +extern float pcCut; /* cut off used for consensus */ + +typedef struct _def_colours { + Pixel lightGrey; +} DefColours; +extern DefColours defColours; + +#define EDITMODE 1 +#define JOINMODE 2 +typedef struct { + int_f *idevr; /* unit number for relationships */ + int_f *idevw; /* unit number for working versions of sequences */ + int_f *idevn; /* unit number for sequence names */ + int_f *idevt; /* unit number for tag information */ + int_f *idevc; /* unit number for comment file */ + int_f *relpg; /* relative positions of gels in sequences */ + int_f *lngthg; /* lengths of sequences */ + int_f *lnbr; /* left neighbours */ + int_f *rnbr; /* right neighbours */ + int_f *maxgel; /* maximum length of gel */ + int_f *idbsiz; /* size of database */ + int_f *lincon; /* current contig to edit */ + int_f *llino; /* left-most gel in contig */ + int_f *lnconl; /* left contig for join */ + int_f *llinol; /* left-most gel in left contig for join */ + int_f *lnconr; /* right contig fro join */ + int_f *llinor; /* left-most gel in right contig for join */ + int_f *perced; /* cutoff for consensus calculation */ + int_f *ngels; /* number of gels in database */ + int_f *nconts; /* number of contigs in database */ + int_f *idm; /* database type */ + } SaveStruct; +extern SaveStruct saveState; + +extern int editorMode; + +extern int editModeIsInsert(); +extern int editModeIsSuperman(); +extern int inJoinMode(); +extern int editorLocked(); +extern int editorLockedPos(int force); +#endif /* _contigEditor_h */ diff --git a/src/staden/copyright.h b/src/staden/copyright.h new file mode 100644 index 0000000..e69de29 diff --git a/src/staden/cvt.c b/src/staden/cvt.c new file mode 100644 index 0000000..c80b7f3 --- /dev/null +++ b/src/staden/cvt.c @@ -0,0 +1,292 @@ +#include +#include +#include +#include +#include +/*#include */ +#include "fort.h" + +#define l_len 100 +#define f_len 200 + +#define MAXDB 1000 + +struct _ar_rec { + int_f idbsiz; + int_f maxgel; + int_f idm; +} AR_rec; + +struct _rl_rec { + int_f ngels; + int_f nconts; + int_f dum1; + int_f dum2; +} RL_rec; + +struct _tg_rec { + int_f pos; /* and count */ + int_f len; + int_f com; + int_f type; + int_f next; +} TG_rec; + +#define COMMENT_LENGTH 40 +struct _cc_rec { + int_f next; + char comment[COMMENT_LENGTH]; +} CC_rec; + +struct _cch_rec{ + int_f next; + int_f count; + char comment[COMMENT_LENGTH-sizeof(int_f)]; +} CCH_rec; + +struct _rd_rec{ + int_f len; + int_f lcut; + int_f wlen; + char type[4]; + char name[12]; +} RD_rec; + +int main() +{ + char projectName[l_len]; + char versionNumber[l_len]; + + fprintf(stdout,"Database conversion program\n"); + fprintf(stdout,"Converts *.RD? file to *.TG? and *.CC? files\n\n"); + + fprintf(stdout,"Project name ? "); + gets(projectName); + + fprintf(stdout,"Version ? "); + gets(versionNumber); + + if (process(projectName,versionNumber)) + fprintf(stdout,"Error: conversion aborted.\n"); + else + fprintf(stdout,"Conversion completed.\n"); + + return 0; +} + +int read_ar(char *AR,int_f *idbsiz) +{ + + FILE *AR_fp; + + /* + ** Check AR file exists + */ + if ((AR_fp=fopen(AR,"rb"))==NULL) { + fprintf(stderr,"Cannot open file %s\n",AR); + return 1; + } + + /* + ** Read details from AR file + */ + fseek(AR_fp,(off_t)((MAXDB-1)*sizeof(AR_rec)),/*SEEK_SET*/0); + fread(&AR_rec,sizeof(AR_rec),1,AR_fp); + + if (ferror(AR_fp)) { + fprintf(stderr,"Cannot read file %s\n",AR); + return 1; + } + + fclose(AR_fp); + + *idbsiz = AR_rec.idbsiz; + return 0; +} + +int read_rl(char *RL,int_f idbsiz, int_f * ngels) +{ + + FILE *RL_fp; + + /* + ** Check RL file exists + */ + if ((RL_fp=fopen(RL,"rb"))==NULL) { + fprintf(stderr,"Cannot open file %s\n",RL); + return 1; + } + + /* + ** Read details from RL file + */ + fseek(RL_fp,(off_t)((AR_rec.idbsiz-1)*sizeof(RL_rec)),/*SEEK_SET*/0); + fread(&RL_rec,sizeof(RL_rec),1,RL_fp); + + if (ferror(RL_fp)) { + fprintf(stderr,"Cannot read file %s\n",RL); + return 1; + } + + fclose(RL_fp); + + *ngels = RL_rec.ngels; + return 0; +} + +void write_tg(FILE *fp,int_f rec, int_f pos, int_f len, int_f com, int_f type, int_f next) +{ + TG_rec.pos = pos; + TG_rec.len = len; + TG_rec.com = com; + TG_rec.type = type; + TG_rec.next = next; + + fseek(fp,(off_t)((rec-1)*sizeof(TG_rec)),/*SEEK_SET*/0); + fwrite(&TG_rec,sizeof(TG_rec),1,fp); +} + +void write_cc(FILE *fp,int_f rec, int_f next, char *comment) +{ + CC_rec.next = next; + strncpy(CC_rec.comment,comment,COMMENT_LENGTH); + + fseek(fp,(off_t)((rec-1)*sizeof(CC_rec)),/*SEEK_SET*/0); + fwrite(&CC_rec,sizeof(CC_rec),1,fp); +} + +void write_cc_head(FILE *fp,int_f next, int_f count ) +{ + CCH_rec.next = next; + CCH_rec.count = count; + + fseek(fp,(off_t)0,/*SEEK_SET*/0); + fwrite(&CCH_rec,sizeof(CC_rec),1,fp); +} + +void read_rd(FILE *fp, int_f rec, int_f *len, int_f *lcut, int_f *wlen, char *type, +char *name) +{ + fseek(fp,(off_t)((rec-1)*sizeof(RD_rec)),/*SEEK_SET*/0); + fread(&RD_rec,sizeof(RD_rec),1,fp); + + *len = RD_rec.len; + *lcut = RD_rec.lcut; + *wlen = RD_rec.wlen; + strncpy(type,RD_rec.type,4); + strncpy(name,RD_rec.name,12); + +} + +int process(char *name,char *vers) +{ + char AR[f_len]; + char RD[f_len]; + char CC[f_len]; + char TG[f_len]; + char RL[f_len]; + + FILE *RD_fp; + FILE *CC_fp; + FILE *TG_fp; + + struct stat statBuff; + + int_f IDBSIZ; + int_f NGELS; + + if (!*vers) strcpy(vers,"0"); + + /* convert bits to upper case */ + { + char *s; + for (s=name; *s = islower(*s)?toupper(*s):*s ;s++); + for (s=vers; *s = islower(*s)?toupper(*s):*s ;s++); + } + + /* + ** create file names + */ + strcpy(RD,name); strcat(RD,".RD"); strncat(RD,vers,1); + strcpy(TG,name); strcat(TG,".TG"); strncat(TG,vers,1); + strcpy(CC,name); strcat(CC,".CC"); strncat(CC,vers,1); + + /* + ** Get AR details + */ + strcpy(AR,name); strcat(AR,".AR"); strncat(AR,vers,1); + if (read_ar(AR,&IDBSIZ)) return 1; + + /* + ** Get RL details + */ + strcpy(RL,name); strcat(RL,".RL"); strncat(RL,vers,1); + if (read_rl(RL,IDBSIZ, &NGELS)) return 1; + + /* + ** open files TG and CC + */ + if ( stat(TG,&statBuff) >= 0 ) { + fprintf(stderr,"%s already exists\n",TG); + return 1; + } + + if ( stat(CC,&statBuff) >= 0 ) { + fprintf(stderr,"%s already exists\n",CC); + return 1; + } + + if ((TG_fp=fopen(TG,"wb"))==NULL) { + fprintf(stderr,"cannot open %s for writing\n",TG); + return 1; + } + + if ((CC_fp=fopen(CC,"wb"))==NULL) { + fprintf(stderr,"cannot open %s for writing\n",CC); + fclose(TG_fp); + return 1; + } + + /* + ** Check RD file exists + */ + if ((RD_fp=fopen(RD,"rb"))==NULL) { + /* + ** None: + ** Create anyway + */ + write_tg(TG_fp,IDBSIZ/*rec*/,IDBSIZ,0,0,0,0); + write_cc_head(CC_fp,0/*next*/,1/*count*/); + + } else { + /* + ** Do the hard graft + */ + int i; + + write_tg(TG_fp,IDBSIZ/*rec*/,IDBSIZ,0,0,0,0); + write_cc_head(CC_fp,0/*next*/,NGELS+1/*count*/); + + for (i=1;i<=NGELS;i++) { + int_f len; + int_f lcut; + int_f wlen; + char type[5]; + char name[13]; + char comment[COMMENT_LENGTH]; + + read_rd(RD_fp,i,&len,&lcut,&wlen,type,name); + type[4] = '\0'; + name[12] = '\0'; + + sprintf(comment,"%6d%6d%6d%-4s%-18s",len,lcut,wlen,type,name); + + write_tg(TG_fp,i,0,0,i+1,0,0); + write_cc(CC_fp,i+1,0,comment); + } + } + + fclose(TG_fp); + fclose(CC_fp); + return 0; +} diff --git a/src/staden/dap.f b/src/staden/dap.f new file mode 100644 index 0000000..dc028c6 --- /dev/null +++ b/src/staden/dap.f @@ -0,0 +1,656 @@ +C SAP (Sequence assembly program) +C AUTHOR RODGER STADEN +C 9-1-90 sent idev instead of kbout to join and enter +C 16-3-90 removed escape after bpause +C 2-5-90 Many changes related to introducing graphics +C 4-5-90 Allowed escape from getreg +C 9-5-90 Introduced a default gel reading +C 23-8-90 Added calls to SHOWFU +C 28-11-90 See dbsys89 +C 3-12-90 Add invocation of contig editor +C 25-02-91 tag and comment files +C 28-2-91 changed maxsav from 1000 to maxglm +C 30-4-91 changes help references to DAP* +C 28-7-91 added extra parameter for quality calc: maxgood, only the parts +C of reads upto this length are used +C 21-8-91 Added an option to find internal joins: AUTOJ +C 27-8-91 Added buffer flushing for database files +C 1-11-91 Upped MAXSEQ to 150000 + SUBROUTINE FMAIN() + INTEGER CHRSIZ,ECHRSZ + PARAMETER ( + + NAMLEN = 40, + + MAXDEV = 12, + + MAXSEQ=150000, + + MAXGLM=4096, + + MAXGL2=MAXGLM*2, + + MINMEN=-3, + + CHRSIZ=4, + + ECHRSZ = 6, + + IDT1=ECHRSZ*MAXGL2, + + IDT2=2*CHRSIZ*MAXGL2, + + IDT3=2*MAXGL2, + + IDT4=2*ECHRSZ*MAXGL2, + + MAXDB=1000) + INTEGER BOTOPT,TOPOPT,DEVNOS(MAXDEV) + INTEGER RELPG(MAXDB),LREG,RREG,ANS + INTEGER TEMP1(IDT1),TEMP2(IDT2),TEMP3(IDT4) + INTEGER LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER*(NAMLEN) HELPF,POINTF,FILNAM,NAMPRO,FILE,NAMARC,FILMAR + CHARACTER*(NAMLEN) SHONAM + CHARACTER GEL(MAXGLM) + CHARACTER GEL2(MAXGLM),GEL3(MAXGLM),GEL4(MAXGLM) + PARAMETER (BOTOPT=0,TOPOPT=35) + PARAMETER (HELPF='DAPHELP') + PARAMETER (POINTF='DAPHPNT') + PARAMETER (FILMAR='DAPMARG') + INTEGER HELPS(BOTOPT:TOPOPT),HELPE(BOTOPT:TOPOPT) + INTEGER MARGB(TOPOPT),MARGT(TOPOPT) + CHARACTER SEQ1(MAXSEQ) +C DBAUTO TEMP3A = TEMP1, SEQ3A = GEL3, SEQ2A = GEL2 +C MATCHA = GEL, SEQ4A = GEL4 + PARAMETER (LENGTH = 6, + + MAXCON = 500, + + MAXSAV = MAXGLM, + + LPOWRC = CHRSIZ**LENGTH) + INTEGER CONST(LENGTH) + INTEGER POSNS(MAXSEQ),WORDP(LPOWRC),WORDN(LPOWRC),GELN(MAXGLM) + INTEGER CENDS(MAXCON),NENDS(MAXCON) + CHARACTER SEQC2(MAXGLM,2),SEQG2(MAXGLM,2) + CHARACTER SEQ5(MAXGLM),SEQG3(MAXGLM),SEQC3(MAXGLM) + INTEGER SAV1(MAXSAV),SAV2(MAXSAV),SAV3(MAXSAV) + INTEGER WINDOW +C DBAUTO + EQUIVALENCE (TEMP2,TEMP1) + EQUIVALENCE (TEMP1,TEMP3) + COMMON /DEVILS/ IDEVT,IDEVC,IDBSIZ,RELPG + IDM = 5 + CALL INITS + CALL INITLU(IDM) + CALL INTHLP('dap', TOPOPT) + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + CALL OPENGR(DEVNOS(10)) + WRITE(KBOUT,1000) +1000 FORMAT( + +' DAP (Development assembly program) V5.1 Aug 1992',/, + +' Author: Rodger Staden') + IGORT = 0 + IDEV = KBOUT +C GET SCREEN AND MARGIN SIZES + CALL GETMRG(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + +TOPOPT,DEVNOS(5),FILMAR) + CALL SETHLP(HELPS,HELPE,BOTOPT,TOPOPT,POINTF,DEVNOS(5),KBOUT) + CALL INITGR(KBIN,KBOUT,HELPS(0),HELPE(0),HELPF,IDEVH) + IOPEN=1 + LINLEN=50 + PERCD=0.75 + WINDOW = 25 + MXGOOD = MAXGLM + IGWIND = 1000 + FILE = ' ' + MAXGEL = MAXGLM + IDEVT = DEVNOS(11) + IDEVC = DEVNOS(12) + IDBSIZ=MAXDB + IDBSIS=IDBSIZ + SHONAM = ' ' + CALL SHOWFI(KBOUT,SHONAM) + ANS = 0 + CALL YESONO(ANS,'Open existing database','Start new database', + + HELPS(3),HELPE(3),HELPF,DEVNOS(5),KBIN,KBOUT) + IF(ANS.LT.0)GO TO 10 + IF(ANS.GT.1)GO TO 10 + IF(ANS.EQ.1)THEN +C CALL SHOWFU(KBOUT,'Start new database') + CALL DBSTAR(NAMPRO,GEL,IDBSIS,IDBSIZ,KBIN,KBOUT,DEVNOS(1), + + DEVNOS(2),DEVNOS(3),IDEVT,IDEVC, + + IERR,HELPS(NOPT),HELPE(NOPT), + + DEVNOS(5),HELPF,MAXGEL,MAXGLM,IDM) + NGELS=0 + NCONTS=0 + LLINO = 0 + IF(IERR.EQ.0) THEN + IOPEN=0 + SHONAM = NAMPRO(1:MAX(1,INDEX(NAMPRO,'.'))+1) + CALL SHOWFI(KBOUT,SHONAM) + END IF + CALL INITLU(IDM) + GO TO 10 + END IF + IF(ANS.EQ.0)THEN +C CALL SHOWFU(KBOUT,'Open database') + NGELS=0 + NCONTS=0 + CALL DBOPEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,NAMPRO,GEL, + + IDBSIS,IDBSIZ,JERR,KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + + IDEVT,IDEVC,MAXGEL,MAXGLM,LLINO,IDM, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(JERR.EQ.0)IOPEN=0 + IF(NGELS.GT.0)CALL DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,KBOUT) + CALL INITLU(IDM) + SHONAM = NAMPRO(1:MAX(1,INDEX(NAMPRO,'.'))+1) + CALL SHOWFI(KBOUT,SHONAM) + GO TO 10 + END IF + MENUN = 0 +10 CONTINUE +C +C Flush the database buffers, just in case! +C + IF (IOPEN.EQ.0) THEN + CALL FLUSHL(DEVNOS(1)) + CALL FLUSHL(DEVNOS(2)) + CALL FLUSHL(DEVNOS(3)) + CALL FLUSHL(IDEVT) + CALL FLUSHL(IDEVC) + END IF + CALL BPAUSE(KBIN,KBOUT,IOK) +C CALL SHOWFU(KBOUT,' ') +C IF(IOK.NE.0) GO TO 9999 +C CALL DBMENT(MENU,NOPT,TOPOPT,HELPS(0),HELPE(0),HELPF,DEVNOS(5), +C +KBIN,KBOUT) + CALL MENU(NOPT, KOPT, MENUN, TOPOPT, MINMEN, KBIN, KBOUT, + + HELPS(0), HELPE(0), HELPF, DEVNOS(5)) + IF(NOPT.EQ.3)THEN + ANS = 0 + CALL YESONO(ANS,'Open existing database','Start new database', + + HELPS(3),HELPE(3),HELPF,DEVNOS(5),KBIN,KBOUT) + IF(ANS.LT.0)GO TO 10 + IF(ANS.GT.1)GO TO 10 + MAXGEL = MAXGLM + IF(IOPEN.EQ.0)THEN + CLOSE(UNIT=DEVNOS(1)) + CLOSE(UNIT=DEVNOS(2)) + CLOSE(UNIT=DEVNOS(3)) + CLOSE(UNIT=IDEVC) + CLOSE(UNIT=IDEVT) + IOPEN=1 + END IF + IDBSIZ=MAXDB + IDBSIS=IDBSIZ + IF(ANS.EQ.1)THEN +C CALL SHOWFU(KBOUT,'Start a database') + CALL DBSTAR(NAMPRO,GEL,IDBSIS,IDBSIZ,KBIN,KBOUT,DEVNOS(1), + + DEVNOS(2),DEVNOS(3),IDEVT,IDEVC, + + IERR,HELPS(NOPT),HELPE(NOPT), + + DEVNOS(5),HELPF,MAXGEL,MAXGLM,IDM) + NGELS=0 + NCONTS=0 + IF(IERR.EQ.0)IOPEN=0 + CALL INITLU(IDM) + SHONAM = NAMPRO(1:MAX(1,INDEX(NAMPRO,'.'))+1) + CALL SHOWFI(KBOUT,SHONAM) + GO TO 10 + END IF + IF(ANS.EQ.0)THEN +C CALL SHOWFU(KBOUT,'Open database') + NGELS=0 + NCONTS=0 + CALL DBOPEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,NAMPRO,GEL, + + IDBSIS,IDBSIZ,JERR,KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + + IDEVT,IDEVC,MAXGEL,MAXGLM,LLINO,IDM, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(JERR.EQ.0)IOPEN=0 + IF(NGELS.GT.0)CALL DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,KBOUT) + CALL INITLU(IDM) + SHONAM = NAMPRO(1:MAX(1,INDEX(NAMPRO,'.'))+1) + CALL SHOWFI(KBOUT,SHONAM) + GO TO 10 + END IF + END IF +C STOP + IF(NOPT.EQ.2)THEN + IF(NGELS.GT.0)CALL DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,KBOUT) + GO TO 9999 + END IF + + +C clear all +C + IF(NOPT.EQ.10)THEN +C CALL SHOWFU(KBOUT,'Clear graphics') + CALL CLEARG + GO TO 10 + END IF +C clear VT100 +C + IF(NOPT.EQ.11)THEN +C CALL SHOWFU(KBOUT,'Clear text') + CALL CLEARV + GO TO 10 + END IF +C +C xhairs +C + IF(NOPT.EQ.13) THEN +C CALL SHOWFU(KBOUT,'Use xhair') + CALL XHSAP(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,NCONTS,LLINO,LINCON,LREG,RREG, + + WINDOW,IGWIND,LENCON,IDEPTH,JDEPTH, + + MARGL,MARGR,MARGB,MARGT,TOPOPT,ISXMAX,ISYMAX,KBIN,IDEV, + + KBOUT,GEL,GEL2,DEVNOS(2),DEVNOS(3),LINLEN,PERCD,MAXGEL,IDM, + + SEQ1,MAXSEQ,NGELS,TEMP3,ECHRSZ,MAXGL2,GEL3,GEL4,33,34,29, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MXGOOD) + GO TO 10 + END IF +C +C CHANGE MARGINS +C + IF(NOPT.EQ.14)THEN +C CALL SHOWFU(KBOUT,'Change margins') + CALL MARGC(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + + HELPS(NOPT),HELPE(NOPT),TOPOPT,HELPF,DEVNOS(5),KBIN,KBOUT) + GO TO 10 + END IF +C ruler +C + IF(NOPT.EQ.12) THEN + LENCON = RREG - LREG + 1 + IF(LENCON.GT.0) THEN +C CALL SHOWFU(KBOUT,'Show ruler') + CALL RULER(LREG,RREG,MARGL,MARGR, + + MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX,KBIN,KBOUT,1, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + GO TO 10 + END IF + END IF +C labler + IF(NOPT.EQ.15)THEN +C CALL SHOWFU(KBOUT,'Label diagram') + CALL LABLER(KBIN,KBOUT,ISXMAX,ISYMAX, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + GO TO 10 + END IF + IF(NOPT.EQ.16) THEN +C CALL SHOWFU(KBOUT,'Plot map') +C map + LENCON = RREG - LREG + 1 + IF(LENCON.GT.0) THEN + CALL PLTMAP(DEVNOS(4),FILNAM,LENCON,MARGL,MARGR,MARGB(NOPT), + + MARGT(NOPT),ISXMAX,ISYMAX,LREG,RREG,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + END IF + GO TO 10 + END IF +C IF((NOPT.EQ.21).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Enter new gel reading') +CC ENTER +C CALL ENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, +C + GEL,GEL2,IDBSIZ,IDEV,KBIN,KBOUT,DEVNOS(1),DEVNOS(2), +C + DEVNOS(3),DEVNOS(4),LINLEN,PERCD,FILNAM, +C + HELPS(NOPT),HELPE(NOPT),HELPS(4),HELPE(4),HELPF,DEVNOS(5), +C + MAXGEL,IDM,LLINO) +C GO TO 10 +C END IF + IF((NOPT.EQ.25).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Show relationships') +C PRINT DB + CALL DBPRNT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ,IDEV, + + KBIN,KBOUT,DEVNOS(3),LLINO, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + GO TO 10 + END IF + IF((NOPT.EQ.5).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Display contig') +C DISPLAY + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + + LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + 1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IERR) + IF(IERR.EQ.0)THEN + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + + GEL,LLINO,LINCON,LREG,RREG,GEL2,I1,I2,0,I,IDBSIZ,IDEV,KBOUT, + + DEVNOS(2),DEVNOS(3),LINLEN,PERCD,MAXGEL,IDM) + END IF + END IF + GO TO 10 + END IF +200 CONTINUE +C IF((NOPT.EQ.-22).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Join contigs') +CC JOIN +C WRITE(KBOUT,1006) +C1006 FORMAT(' Which contigs do you want to join ?') +C WRITE(KBOUT,1017) +C1017 FORMAT( ' Left contig') +C LLINOL = 0 +C LLINOR = 0 +C CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LNCONL,LLINOL, +C + IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), +C + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) +C IF(IERR.EQ.0)THEN +C WRITE(KBOUT,1018) +C1018 FORMAT( ' Right contig') +C CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, +C + LNCONR,LLINOR,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), +C + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) +C IF(IERR.EQ.0)THEN +CC CHECK TO SEE IF MAKING A CIRCLE! +C IF(LLINOL.EQ.LLINOR)THEN +C WRITE(KBOUT,*)' Making circles is not allowed!' +C GO TO 200 +C END IF +C CALL JOIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, +C + LNCONL,LLINOL,LNCONR,LLINOR,GEL,GEL2, +C + IDBSIZ,IDEV,KBIN,KBOUT,DEVNOS(1),DEVNOS(2), +C + DEVNOS(3),LINLEN,PERCD, +C + HELPS(NOPT),HELPE(NOPT),HELPS(4),HELPE(4),HELPF,DEVNOS(5), +C + MAXGEL,IDM) +C END IF +C END IF +C GO TO 10 +C END IF + IF((NOPT.EQ.23).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Complement contig') +C COMPLEMENT + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + LINCON,LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL CMPLMT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + LINCON,LLINO,GEL,IDBSIZ,KBOUT,DEVNOS(1),DEVNOS(2), + + MAXGEL) + END IF + GO TO 10 + END IF +CC EDIT +C IF((NOPT.EQ.-4).AND.(IOPEN.EQ.0))THEN +CC EDIT DB +C CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, +C + LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), +C + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) +C IF(IERR.EQ.0)THEN +C CALL DBEDIT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, +C + GEL,LINCON,0,0,IDBSIZ,KBIN,KBOUT,DEVNOS(1),DEVNOS(2), +C + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MAXGEL) +C END IF +C GO TO 10 +C END IF +C PLOT SINGLE CONTIG + IF((NOPT.EQ.33).AND.(IOPEN.EQ.0))THEN + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + + LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + 1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IERR) + IF(IERR.EQ.0)THEN + LENCON = RREG - LREG + 1 + CALL FDEPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX) + END IF + END IF + GO TO 10 + END IF + IF((NOPT.EQ.26).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Alter relationships') +C FIX + CALL DBFIX(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + GEL,GEL2,IDBSIZ,KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT), + + HELPS(4),HELPE(4),HELPF,DEVNOS(5),MAXGEL,IDEVT,IDEVC) + GO TO 10 + END IF + IF((NOPT.EQ.24).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Copy database') +C COPY + CALL DBCOPY(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,GEL, + + NAMPRO,DEVNOS(4),IDBSIZ,JERR,KBIN,KBOUT,DEVNOS(1), + + DEVNOS(2),DEVNOS(3),IDEVT,IDEVC, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MAXGEL,MAXDB,IDM) + GO TO 10 + END IF + IF((NOPT.EQ.19).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Check database for consistency') +C CHECK + CALL DBCHEK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + TEMP1,IERR,KBOUT) + GO TO 10 + END IF + IF((NOPT.EQ.29).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Examine quality') +C SCAN + IDIM1=MAXSEQ + CALL DBSCAN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1, + + IDIM1,GEL,IDBSIZ,TEMP3,2,ECHRSZ,MAXGL2, + + KBIN,KBOUT,DEVNOS(2),IDEV,LINLEN,PERCD, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MAXGEL,GEL3,GEL4, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX,DEVNOS(3), + + LLINO,LINCON,LREG,RREG,MXGOOD) + GO TO 10 + END IF + IF((NOPT.EQ.8).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Make consensus') +C CONSENSUS + IDIM1=MAXSEQ + CALL CONSEN(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS,SEQ1, + + IDIM1,GEL,IDBSIZ,TEMP1,ECHRSZ,MAXGL2, + + KBIN,KBOUT,DEVNOS(2),DEVNOS(4),FILNAM, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MAXGEL,IDM,PERCD, + + DEVNOS(3),LLINO) + CLOSE(UNIT=DEVNOS(4)) + GO TO 10 + END IF + IF(NOPT.EQ.7)THEN +C CALL SHOWFU(KBOUT,'Redirect output') + CALL REDIR(IDEV,DEVNOS(6),DEVNOS(10),IGORT,FILNAM,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),KOPT) + GO TO 10 + END IF +C HELP + IF(NOPT.EQ.1)THEN +C CALL SHOWFU(KBOUT,'Help') +C HELP + CALL HELP(HELPS,HELPE,BOTOPT,TOPOPT, + + HELPF,DEVNOS(5),KBIN,KBOUT) + GO TO 10 + END IF + IF(NOPT.EQ.27)THEN +C CALL SHOWFU(KBOUT,'Set parameters') +C SET DISPLAY PARAMETERS + MN = 10 + MX = 100 + CALL GETINT(MN,MX,LINLEN,'Display line length', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) LINLEN = IVAL + IPCMIN = 51 + IPCMAX = 100 + IPCD = INT(PERCD*100.) + CALL GETINT(IPCMIN,IPCMAX,IPCD, + + 'Percentage score for consensus', + + IPVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) THEN + PERCD = REAL(IPVAL)/100. + END IF + MN = 1 + MX = MAXGLM + CALL GETINT(MN,MX,MXGOOD, + + 'Maximum read length for quality analysis', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) MXGOOD = IVAL + MN = 1 + MX = MAXSEQ + CALL GETINT(MN,MX,WINDOW,'Text window length', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) WINDOW = IVAL + MN = 1 + MX = MAXSEQ + CALL GETINT(MN,MX,IGWIND,'Graphics window length', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) IGWIND = IVAL + GO TO 10 + END IF + IF(NOPT.EQ.6)THEN +C CALL SHOWFU(KBOUT,'Display text file') + CALL TTEXT(DEVNOS(4),FILNAM,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + CLOSE(UNIT=DEVNOS(4)) + GO TO 10 + END IF + IF(NOPT.EQ.28) THEN +C CALL SHOWFU(KBOUT,'Highlight disagreements') + CALL HIGHLT(SEQ1,SEQ1(6001),SEQ1(10001),KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),DEVNOS(7), + + DEVNOS(8),FILE,IOK) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + GO TO 10 + END IF + IF((NOPT.EQ.34).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Plot contigs') +C PLOT ALL CONTIGS + CALL PLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,0,0, + + NCONTS,MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX) + GO TO 10 + END IF +CC AUTO EDIT +C IF((NOPT.EQ.30).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'AutoEdit a contig') +C WRITE(KBOUT,*)'Make a copy first!' +C CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, +C + LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), +C + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) +C IF(IERR.EQ.0)THEN +C CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, +C + 1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, +C + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IERR) +C IF(IERR.EQ.0) THEN +C IDIM1=MAXSEQ +C CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1,IDIM1, +C + GEL,LREG,RREG,LLINO,IDBSIZ,TEMP1,ECHRSZ,MAXGL2, +C + DEVNOS(2),MAXGEL,IDM,PERCD) +C CALL AEDIT(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LLINO,LINCON, +C + GEL,MAXGEL,SEQ1,IDIM1,DEVNOS(2),DEVNOS(1),LREG,RREG, +C + KBOUT) +C END IF +C END IF +C GO TO 10 +C END IF +C IF((NOPT.EQ.9).AND.(IOPEN.EQ.0)) THEN +C CALL SHOWFU(KBOUT,'Screen Edit a contig') +C CALL CEDT(KBIN,KBOUT, +C + POSNS(1),POSNS(1001),POSNS(2001), +C + MAXDB,POSNS(3001),POSNS(3101),50, +C + RELPG,LNGTHG,LNBR,RNBR, +C + IDBSIZ,NGELS,NCONTS,GEL,GEL2,MAXGEL,LINCON,PERCD,IDM, +C + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),DEVNOS(4), +C + DEVNOS(1),DEVNOS(2),DEVNOS(3),LINLEN,FILNAM,LLINO,IOK) +C GO TO 10 +C END IF + IF((NOPT.EQ.4).AND.(IOPEN.EQ.0))THEN +C CALL UP CONTIG EDITOR + CALL CONEDT(KBIN,KBOUT, + + POSNS(1),POSNS(1001),POSNS(2001), + + MAXDB,POSNS(3001),POSNS(3101),50, + + RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,NGELS,NCONTS,GEL,GEL2,MAXGEL,LINCON,PERCD,IDM, + + HELPS(9),HELPE(9),HELPF,DEVNOS(5),DEVNOS(4), + + DEVNOS(1),DEVNOS(2),DEVNOS(3),IDEVT,IDEVC, + + LINLEN,FILNAM,LLINO,IOK, + + IERR,TEMP1) + GO TO 10 + END IF + IF((NOPT.EQ.22).AND.(IOPEN.EQ.0))THEN +C CALL UP JOIN EDITOR + CALL JOINED(KBIN,KBOUT, + + POSNS(1),POSNS(1001),POSNS(2001), + + MAXDB,POSNS(3001),POSNS(3101),50, + + RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,NGELS,NCONTS,GEL,GEL2,MAXGEL,LINCON,PERCD,IDM, + + HELPS(9),HELPE(9),HELPF,DEVNOS(5),DEVNOS(4), + + DEVNOS(1),DEVNOS(2),DEVNOS(3),IDEVT,IDEVC, + + LINLEN,FILNAM,LLINO,IOK, + + IERR,TEMP1) + GO TO 10 + END IF + IF(NOPT.EQ.17) THEN +C CALL SHOWFU(KBOUT,'Screen for restriction sites') + CALL SCRENR(GEL2,MAXGEL,GEL3,NAMARC,FILE, + + DEVNOS(4),DEVNOS(7),DEVNOS(8),DEVNOS(9),IDEV,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + CLOSE(UNIT=DEVNOS(4)) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + CLOSE(UNIT=DEVNOS(9)) + GO TO 10 + END IF + IF(NOPT.EQ.18) THEN +C CALL SHOWFU(KBOUT,'Screen against vector') + CALL SCRENV(MAXGEL,WORDP,WORDN,LPOWRC,POSNS,GELN, + + SEQ1,MAXSEQ,GEL2,GEL3,GEL4,LENGTH, + + SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON,CONST, + + KBIN,KBOUT,DEVNOS(4),DEVNOS(7),DEVNOS(8),DEVNOS(9),IDEV, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),NAMARC,FILE,IOK) + CLOSE(UNIT=DEVNOS(4)) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + CLOSE(UNIT=DEVNOS(9)) + GO TO 10 + END IF + IF((NOPT.EQ.20).AND.(IOPEN.EQ.0).AND.(IDM.EQ.5)) THEN +C CALL SHOWFU(KBOUT,'Auto assemble sequences') + CALL DBAUTO(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ, + +NGELS,NCONTS,MAXGEL, + +TEMP1,WORDP,WORDN,LPOWRC,POSNS,GELN, + +SEQ1,MAXSEQ,GEL2,GEL3,GEL4,SEQ5,SEQC2,SEQG2,GEL, + +MAXGLM,MAXGL2,CHRSIZ,ECHRSZ,LENGTH, + +SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON,CONST, + +KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + +DEVNOS(4),DEVNOS(7),DEVNOS(8),IDEV,IDEVC,IDEVT, + +HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),NAMARC,NAMPRO,FILE, + +PERCD,IOPEN,IDM,SEQG3,SEQC3,IOK) + CLOSE(UNIT=DEVNOS(4)) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + GO TO 10 + END IF + IF((NOPT.EQ.35).AND.(IOPEN.EQ.0).AND.(IDM.EQ.5)) THEN +C CALL SHOWFU(KBOUT,'Find internal joins') + CALL AUTOJ(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ, + +NGELS,NCONTS,MAXGEL, + +TEMP1,WORDP,WORDN,LPOWRC,POSNS,GELN, + +SEQ1,MAXSEQ,GEL2,GEL3,GEL4,SEQ5,SEQC2,SEQG2,GEL, + +MAXGLM,MAXGL2,CHRSIZ,ECHRSZ,LENGTH, + +SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON,CONST, + +KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + +DEVNOS(4),DEVNOS(7),DEVNOS(8),IDEV, + +HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),NAMARC,NAMPRO,FILE, + +PERCD,IOPEN,IDM,SEQG3,SEQC3,IOK) + CLOSE(UNIT=DEVNOS(4)) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + GO TO 10 + END IF + IF((NOPT.EQ.32).AND.(IOPEN.EQ.0)) THEN +C CALL SHOWFU(KBOUT,'Extract gel readings') + CALL GELOUT(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ,NGELS, + + NCONTS,GEL,MAXGEL,DEVNOS(2),DEVNOS(3),DEVNOS(4),DEVNOS(7), + + KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),FILE) + CLOSE(UNIT=DEVNOS(4)) + GO TO 10 + END IF +C IF(NOPT.EQ.31) THEN +C CALL SHOWFU(KBOUT,'Type in gel readings') +C CALL BATIN(SEQ1,MAXSEQ,KBIN,KBOUT, +C + DEVNOS(4),DEVNOS(7),IDEV, +C + FILNAM,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) +C GO TO 10 +C END IF + IF((NOPT.GT.3).AND.(IOPEN.NE.0))THEN + WRITE(KBOUT,1012) +1012 FORMAT(' You have not opened a database!') + GO TO 10 + END IF + GO TO 10 +9999 CONTINUE + CALL SHUTD + END diff --git a/src/staden/dbchek.f b/src/staden/dbchek.f new file mode 100644 index 0000000..ad3c838 --- /dev/null +++ b/src/staden/dbchek.f @@ -0,0 +1,133 @@ +C DBCHEK +C TO CHECK THE SELF CONSISTENCY OF A DB +C ARE ALL NEIGHBOURS HOLDING HANDS OR DO SOME HAVE +C GELS WHICH DO NOT BELONG TO ANY CONTIG? OR WORSE BELONG TO MORE +C THAN ONE CONTIG? +C ARE THERE ANY LOOPS IN CONTIGS (THE WORST PROBLEM) +C ARE ALL RELATIVE POSITIONS IN SAME ORDER AS NEIGHBOURS? +C ARE THERE ANY CONTIGS OF LENGTH <1 ? +C ARE THERE ANY GELS OF ZERO LENGTH ? +C RETURN ERROR CODE OF ZERO FOR ALL OK, 1 FOR GELS NOT USED +C AND 2 FOR ALL OTHER ERRORS + SUBROUTINE DBCHEK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +TEMP,IERR,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER TEMP(IDBSIZ) + IERR=0 +C HAND HOLDING OK? + DO 100 I=1,NGELS + K=LNBR(I) + IF(K.EQ.0)GO TO 50 + IF(RNBR(K).EQ.I)GO TO 50 + WRITE(KBOUT,1000)I +1000 FORMAT(' Hand holding problem for gel reading',I6) + WRITE(KBOUT,1001)I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) + WRITE(KBOUT,1001)K,RELPG(K),LNGTHG(K),LNBR(K),RNBR(K) +1001 FORMAT(' ',5I6) + IERR=2 +50 CONTINUE + K=RNBR(I) + IF(K.EQ.0)GO TO 100 + IF(LNBR(K).EQ.I)GO TO 100 + WRITE(KBOUT,1000)I + WRITE(KBOUT,1001)I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) + WRITE(KBOUT,1001)K,RELPG(K),LNGTHG(K),LNBR(K),RNBR(K) + IERR=2 +100 CONTINUE +C +C ALL GELS IN CONTIGS OR IN MORE THAN ONE CONTIG? +C TEMP IS USED TO COUNT THE NUMBER OF TIMES EACH GEL IS USED + CALL FILLI(TEMP,IDBSIZ,0) + N=IDBSIZ-NCONTS +C COUNT NUMBER OF PASSES THRU LOOP 320 TO 300. IF THIS EXCEEDS +C THE DATABASE SIZE A LOOP HAS BEEN FOUND! + ICOUNT=0 + DO 300 I=N,IDBSIZ-1 + II=I + K=LNBR(I) + J=RNBR(I) + IF((K.NE.0).AND.(J.NE.0))GO TO 310 +C THIS CONTIG POINTS TO ZERO GEL NUMBER AS LEFT OR RIGHT END! + WRITE(KBOUT,1002)I +1002 FORMAT(' Contig',I4,' has zero gel numbers') + IERR=2 + GO TO 290 +310 CONTINUE + IF((LNBR(K).EQ.0).AND.(RNBR(J).EQ.0))GO TO 290 +C THIS LEFT END IS NOT A LEFT END OR THIS RIGHT END IS NOT A RIGHT END + WRITE(KBOUT,1004)I +1004 FORMAT(' The end gels of contig',I4,' have outward neighbours') + IERR=2 +290 CONTINUE +C DOES THE CONTIG HAVE SOME +VE LENGTH? + IF(RELPG(I).GT.0)GO TO 320 + WRITE(KBOUT,1010)I +1010 FORMAT(' The contig on line number',I4,' has zero length') + IERR=2 +320 CONTINUE + TEMP(K)=TEMP(K)+1 + ICOUNT=ICOUNT+1 + IF(ICOUNT.GT.IDBSIZ)GO TO 601 + K=RNBR(K) + IF(K.NE.0)GO TO 320 +300 CONTINUE + DO 400 I=1,NGELS + IF(TEMP(I).EQ.1)GO TO 390 + IF(TEMP(I).EQ.0)GO TO 410 + WRITE(KBOUT,1005)I,TEMP(I) +1005 FORMAT(' Gel number ',I6,' is used ',I6,' times') + IERR=2 + GO TO 400 +390 CONTINUE +C DOES THE GEL HAVE NONZERO LENGTH? (ONLY CHECK THOSE USED ONCE) + IF(LNGTHG(I).NE.0)GO TO 400 + WRITE(KBOUT,1011)I +1011 FORMAT(' Gel number',I6,' has zero length') + IERR=2 + GO TO 400 +410 CONTINUE + WRITE(KBOUT,1006)I +1006 FORMAT(' Gel number ',I6,' is not used') +C NEED TO INCREMENT THE ERROR COUNT NOT RESET IT TO A LOWER VALUE! + IF(IERR.LT.2)IERR=1 +400 CONTINUE +C ALL RELATIVE POSNS OK? + N=IDBSIZ-NCONTS + DO 500 I=N,IDBSIZ-1 + K=LNBR(I) + IF(K.EQ.0)GO TO 500 +510 CONTINUE + J=RNBR(K) + IF(J.EQ.0)GO TO 500 + IF(RELPG(K).GT.RELPG(J))GO TO 520 + K=J + GO TO 510 +520 CONTINUE + WRITE(KBOUT,1007)K,RELPG(K),J,RELPG(J) +1007 FORMAT(' Gel number',I6,' with position',I6, + + ' is the left neighbour of', + + /,' gel number',I6,' with position',I6) + K=J + IERR=2 + GO TO 510 +500 CONTINUE + IF(IERR.EQ.0)WRITE(KBOUT,1013) +1013 FORMAT(' Database is logically consistent') + RETURN +601 CONTINUE + IERR=2 + WRITE(KBOUT,1008)II +1008 FORMAT(' Loop in contig',I6,/, + +' No further checking done but gel numbers follow') + CALL FILLI(TEMP,IDBSIZ,0) + K=LNBR(II) +710 CONTINUE + TEMP(K)=TEMP(K)+1 + WRITE(KBOUT,1009)K +1009 FORMAT(' ',I6) + IF(TEMP(K).GT.1)RETURN + K=RNBR(K) + GO TO 710 + END diff --git a/src/staden/dbsyscommon.f b/src/staden/dbsyscommon.f new file mode 100644 index 0000000..d7a9b04 --- /dev/null +++ b/src/staden/dbsyscommon.f @@ -0,0 +1,5835 @@ +C DBSYS ROUTINES COMMON TO PRE AND POST .RD PROGRAMS +C 8-4-92 altered gtconc, summer,summar,dsplay +C 6-4-92 bug fix in autocn: i did not copy the correct elements +C for some cases where a better alignment was found: i was +C copying from jlefts instead of ilefts, jlc and jsense. +C Further sap routines are in dbsysold.f (pre .rd) and dbsysnew.f (post) +C the split was made by rs 23-1-91 +C 4-5-90 Change to getreg to allow escape +C 4-5-90 addition of graphics routines and changes to menus +C 9-5-90 added default gel reading: many changes +C 17-5-90 Fixed 3 bugs in screen editing: 1) rightjustified names +C caused problems; 2) beginnings of sequences starting at far +C right of lines where not seen; 3) lines with no numbers at +C the end of a contig (i.e. with <10 chars) were flagged +C as errors. Changes to ltype for 1, linlen for 2, dsplay for 3 +C 9-7-90 removed menu routines +C 20-8-90 changed gelid to add / to reading name because xsap did +C not return the INFLAG = 3 for the default +C 23-8-90 Changes to dbauto and autocn to deal with failures better +C Plus addition of calls to BUSY +C 9-11-90 Replaced call to radio with call to radion +C 19-11-90 Changed max match length in dbauto to maxglm+1 (was 50) +C 25-11-90 Very important bug fix in tpchek. Old versions could +C duplicate bits of working versions. +C 28-11-90 Modified slider to receive maxpg and maxpc and to allow exactly +C the requested number of matches at each end of the two +C sequences. +C Added two new options to dbauto: all gels to new contigs, all +C gels to contig 1; plus resurrected forbidding joins to allow +C sequences to be entered only into the contig the overlap best. +C Changed autocn to sort overlaps into order based on % mismatch +C (previously it saved the best two in any order) +C Minor change to dbstar +C 3-1-91 Discovered bug in dbopen: incorrect call to getint when the +C database is very old and needs values for the current format +C 21-1-91 GELID allowed illegal gel numbers to be returned! Fixed it. +C 22-1-91 Modified autocn, adism4,adism3 to give more info about +C overlaps, and to allow 10 overlaps. Modified dbopen to +C return version number, ditto dbstar +C 23-1-91 Split into dbsyscommon, dbsysold, dbsysnew +C 26-2-91 Improved overflow check in padcop +C 28-7-91 added extra parameter to quality calc: mxgood is the maximum +C reading length in which we have confidence, so only add this +C many chars from the start of each reading. Also changed the +C quality calc to make it the same as the consensus one. Made +C all characters have nonzero score and made lowercase = 100 +C 21-8-91 Changed arrfil to arrfim which does not display comments +C 22-8-91 Added routine to find contig line number given left gel (CLINNO) +C 8-11-91 Added fmt4lp which is used by "find internal joins" and would +C be useful elsewhere as a replacement for fmt4ln +C 18-11-91 New routine GETLN2 with returns gel number specified +C 27-2-92 Added use of ctonum to cfgel to deal with gel and vector +C being different case. +C 2-3-92 set FILNAM = ' ' for some calls to openf1 +C SUBROUTINE TO READ CHARACTER DATA FROM IDEV, REMOVE SPACES, FILL +C ARRAY AND RETURN NUMBER OF ELEMENTS USED. ANY LINES STARTING WITH +C A ; ARE TREATED AS COMMENTS + SUBROUTINE ARRFIM(IDEV,SEQNCE,J,KBOUT) +C 14-8-91 Added err= option to read, and set length to 0 if error found +C AUTHOR: RODGER STADEN + CHARACTER TEMP(80),SEQNCE(J) + CHARACTER SPACE,ENDCHR,TITCHR + SAVE ENDCHR,SPACE,TITCHR + DATA ENDCHR/'@'/ + DATA SPACE/' '/ + DATA TITCHR/';'/ + IDMX=J + J=0 +1 CONTINUE + READ(IDEV,1001,END=30,ERR=40)TEMP +1001 FORMAT(80A1) + IF(TEMP(1).EQ.TITCHR)THEN +C WRITE(KBOUT,1003)(TEMP(K),K=2,80) +C1003 FORMAT(' ',79A1) + GO TO 1 + END IF +10 CONTINUE + DO 20 I=1,80 + IF(TEMP(I).NE.SPACE)THEN + IF(TEMP(I).EQ.ENDCHR)RETURN + IF(J.EQ.IDMX)THEN + WRITE(KBOUT,1002)IDMX +1002 FORMAT( + + ' Too much data. Maximum possible', + + ' =',I6,', input stopped there') + RETURN + END IF + J=J+1 + SEQNCE(J)=TEMP(I) + END IF +20 CONTINUE + GO TO 1 +30 CONTINUE + RETURN + 40 CONTINUE + CALL ERROM(KBOUT,'Error reading file') + J = 0 + END +C ABEDIN +C +C ROUTINE TO EDIT THE DB USING A PADDED SEQ +C HAVE AN ARRAY SEQC2 LENGTH IDC OF PADDED SECTION OF CONTIG LINCON +C THE LEFT END OF THE PADDED CONTIG STARTS AT X +C THERE ARE ITOTPC PADS TO MAKE +C + SUBROUTINE ABEDIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,LINCON,X,SEQC2,ITOTPC,IDC,IDBSIZ,KBOUT,IDEVR,IDEVW, + +MAXGEL) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),X,POSN + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER SEQC2(IDC),GEL(MAXGEL),P + SAVE P + DATA P/','/ +C +C POINT TO CONTIG + POSN=X-1 +C POINT TO SEQC2 + IAT=0 +C COUNT PADS DONE + IDONE=0 +C LOOP FOR ALL SEQC2 + DO 100 J=1,IDC + POSN=POSN+1 + IAT=IAT+1 + IPAD=0 +C IS THIS A PADDING CHAR? + IF(SEQC2(IAT).NE.P)GO TO 100 +50 CONTINUE +C COUNT PADS + IPAD=IPAD+1 + IAT=IAT+1 + IF(SEQC2(IAT).EQ.P)GO TO 50 +C END OF THIS STRETCH OF PADS,DO INSERT +C HAVE IPAD INSERTS TO MAKE AT POSN + CALL PADCON(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,LINCON,POSN,IPAD,IDBSIZ,IDEVR,IDEVW,MAXGEL,KBOUT) +C MOVE POINTER TO CONTIG + POSN=POSN+IPAD +C COUNT PADS DONE + IDONE=IDONE+IPAD +C ANY MORE TO DO? + IF(IDONE.EQ.ITOTPC)GO TO 101 +100 CONTINUE +C ERROR SHOULD HAVE DONE ALL PADS + WRITE(KBOUT,1000) +1000 FORMAT(' Problem: some pads were not done!') +101 CONTINUE + END +C ACONS + SUBROUTINE ACONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,IDBSIZ,ISTART,ANS,LLINO,LREG,RREG,TEMP, + +CHRSIZ,MAXGL2,KBOUT, + +IDEVW,IFAIL,MAXGEL,IDM,PERCD) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),ANS,CHRSIZ + INTEGER LREG,RREG,X,Y,TEMP(CHRSIZ,MAXGL2) + CHARACTER SEQ1(IDIM1) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + CHARACTER NAMPRO*(*) +C WRITE(KBOUT,1000) +C1000 FORMAT(' CALCULATING A CONSENSUS') +C +C +C + CALL BUSY(KBOUT) + IFAIL=0 + IF(ANS.EQ.1)GO TO 150 + N=IDBSIZ-NCONTS + DO 110 I=N,IDBSIZ-1 + J=LNBR(I) + X=1 + Y=RELPG(I) + ISTART=ISTART+1 + IF((ISTART+19+Y).GT.IDIM1)THEN + WRITE(KBOUT,1009)IDIM1 +1009 FORMAT( + +' Database maximum consensus length(',I6,') exceeded',/, + +' calculation aborted') + IFAIL=1 + RETURN + END IF + CALL ADDTIT(SEQ1(ISTART),NAMPRO,J,ISTART) + CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + 1SEQ1(ISTART),Y,GEL,X,Y,J,IDBSIZ,TEMP,CHRSIZ,MAXGL2,IDEVW,MAXGEL, + +IDM,PERCD) + ISTART=ISTART+Y-1 +110 CONTINUE + RETURN +C SELECTED CONTIGS ONLY +150 CONTINUE + ISTART=ISTART+1 + IDIM11=RREG-LREG+1 + IF((ISTART+19+IDIM11).GT.IDIM1)THEN + WRITE(KBOUT,1009)IDIM1 + IFAIL=1 + RETURN + END IF + CALL ADDTIT(SEQ1(ISTART),NAMPRO,LLINO,ISTART) +C +C + CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + 1SEQ1(ISTART),IDIM11,GEL,LREG,RREG,LLINO,IDBSIZ,TEMP, + +CHRSIZ,MAXGL2, + 2IDEVW,MAXGEL,IDM,PERCD) + ISTART=ISTART+IDIM11-1 +C + RETURN + END + SUBROUTINE ADDTIT(SEQ1,NAMPRO,NGELS,IDIM1) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(20),NAMPRO*(*) + CHARACTER NUMS(10) + SAVE NUMS + DATA NUMS/'0','1','2','3','4','5','6','7','8','9'/ + CALL FILLC(SEQ1(2),18,'-') + SEQ1(1) = '<' + SEQ1(20) = '>' + IEND = INDEX(NAMPRO,'.') + N=NGELS + K=IEND+3 + DO 10 J=1,3 + N=MOD(N,10)+1 + NAMPRO(K:K)=NUMS(N) + N=NGELS/(10**J) + K=K-1 +10 CONTINUE + K = 18-IEND + K=K/2 + DO 20 I=1,IEND+3 + SEQ1(K)=NAMPRO(I:I) + K=K+1 +20 CONTINUE + IDIM1=IDIM1+20 + RETURN + END + SUBROUTINE ADISM1(SEQ,IDIM,GEL,IDIMG,SAVPS,SAVPG,IDSAV, + +CENDS,NENDS,IDCEND,MAXCON,ILEFTS,ILC,IPOSC,IPOSG,ISENSE, + +LLINO,IMATC, + +ISTRAN,KBOUT,MATCH) +C AUTHOR: RODGER STADEN +C NEW PARMS + INTEGER ILEFTS(2),ILC(2),IPOSC(2),IPOSG(2),ISENSE(2),LLINO(2) +CCCCCCCCCCCC + INTEGER CENDS(MAXCON) + INTEGER NENDS(MAXCON) + INTEGER SAVPS(IDSAV),SAVPG(IDSAV) + CHARACTER SEQ(IDIM),GEL(IDIMG),MATCH(IDIMG) +C +C EDITED 07-02-83 TO ALLOW FOR CASE WHERE A GEL OVERLAPS ADJACENT +C CONTIGS WITHIN THE LENGTH OF THE GEL. USE PARM THAT CONTAINS +C THE POSITION OF THE LEFT END OF THE NEXT CONTIG. SET TO VERY HIGH +C VALUE TO START + NEXTC=IDIM+1 +C SORT THE MATCHING WORDS INTO ASCENDING ORDER ON POSITION IN SEQ + CALL BUB2AS(SAVPS,SAVPG,IDSAV) +C LOOK FOR SEPERATE MATCHES + LEND=IDIMG-SAVPG(1)+SAVPS(1) +C COUNT NUMBER OF MATCHING CONTIGS + IMATC=IMATC+1 + CALL ADISM2(SEQ,IDIM,GEL,IDIMG,SAVPS(1), + 1SAVPG(1),CENDS,NENDS,IDCEND,MAXCON, + 1ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN,NEXTC,KBOUT, + 2MATCH) + DO 10 I=2,IDSAV + IF((SAVPS(I).LT.LEND).AND.(SAVPS(I).LT.NEXTC))GO TO 10 +C NEW MATCH, DISPLAY IT +C COUNT NUMBER OF MATCHING CONTIGS + IMATC=IMATC+1 + CALL ADISM2(SEQ,IDIM,GEL,IDIMG,SAVPS(I), + 1SAVPG(I),CENDS,NENDS,IDCEND,MAXCON, + 1ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN,NEXTC,KBOUT, + 2MATCH) +C +C RESET LEND + LEND=IDIMG-SAVPG(I)+SAVPS(I) +10 CONTINUE + RETURN + END +C +C ADISM2 +C ROUTINE TO DISPLAY MATCHES + SUBROUTINE ADISM2(SEQ,IDIM1,GEL,IDIMG,ISAVPS,SAVPG,CENDS,NENDS, + +IDCEND,MAXCON,ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN, + +NEXTC,KBOUT,MATCH) +C AUTHOR: RODGER STADEN +C NEW PARMS + INTEGER ILEFTS(2),ILC(2),IPOSC(2),IPOSG(2),ISENSE(2),LLINO(2) +CCCCCCCCCCC + CHARACTER SEQ(IDIM1),GEL(IDIMG),MATCH(IDIMG) + INTEGER SAVPS,SAVPG,CENDS(MAXCON) + INTEGER NENDS(MAXCON) +C EDITED 07-02-83 FOR NEXTC. SEE ADISM1. +C DELETE 20 FROM END OF CONSENSUS MATCH + SAVPS=ISAVPS-19 +C FIND CONTIG CONSENSUS ENDS + JJ=1 + DO 5 J=2,IDCEND + IF(SAVPS.GT.CENDS(J))GO TO 5 +C GONE PAST SO LAST IS THE ONE + JJ=J-1 + GO TO 6 +5 CONTINUE + JJ=IDCEND +6 CONTINUE +C SUBTRACT 1 FROM END + SAVPS=SAVPS-1 +C LENGTH FROM MATCH TO LEFT OF CONTIG + LCL=SAVPS-CENDS(JJ) +C RIGHT + LCR=CENDS(JJ+1)-ISAVPS-1 +C LEFT GEL + LGL=SAVPG-1 + LGR=IDIMG-SAVPG +C NEED MIN OF EACH PAIR + LL=MIN(LCL,LGL) + LR=MIN(LCR,LGR) +C LENGTH OF OVERLAP + LM=LR+LL+1 +C DISPLAY STARTS + ICL=ISAVPS-LL + IGL=SAVPG-LL + WRITE(KBOUT,1000)NENDS(JJ) +1000 FORMAT(' Match found with contig number =',I6) + CALL SQMTCH(SEQ(ICL),GEL(IGL),MATCH,LM) + L=ICL-CENDS(JJ)-19 + CALL FMT4LN(SEQ(ICL),GEL(IGL),MATCH,LM,L,IGL,KBOUT) +C UPDATE END OF NEXT CONTIG + NEXTC=CENDS(JJ+1)+20 + IF(IMATC.GT.2)RETURN + ILEFTS(IMATC)=CENDS(JJ)+20 + ILC(IMATC)=LCL+LCR+1 + IPOSC(IMATC)=LCL+1 + IPOSG(IMATC)=SAVPG + LLINO(IMATC)=NENDS(JJ) + ISENSE(IMATC)=1 + IF(ISTRAN.EQ.2)ISENSE(IMATC)=-1 + RETURN + END + SUBROUTINE ADISM3(ISAVPS,SAVPG,CENDS,NENDS, + +IDCEND,MAXCON,ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN, + +NEXTC,MAXC,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER ILEFTS(MAXC),ILC(MAXC),IPOSC(MAXC),IPOSG(MAXC) + INTEGER ISENSE(MAXC),LLINO(MAXC) + INTEGER SAVPS,SAVPG,CENDS(MAXCON) + INTEGER NENDS(MAXCON) + SAVPS=ISAVPS-19 + JJ=1 + DO 5 J=2,IDCEND + IF(SAVPS.GT.CENDS(J))GO TO 5 + JJ=J-1 + GO TO 6 +5 CONTINUE + JJ=IDCEND +6 CONTINUE + SAVPS=SAVPS-1 + LCL=SAVPS-CENDS(JJ) + LCR=CENDS(JJ+1)-ISAVPS-1 + NEXTC=CENDS(JJ+1)+20 + IF(IMATC.LE.MAXC) THEN + ILEFTS(IMATC)=CENDS(JJ)+20 + ILC(IMATC)=LCL+LCR+1 + IPOSC(IMATC)=LCL+1 + IPOSG(IMATC)=SAVPG + LLINO(IMATC)=NENDS(JJ) + ISENSE(IMATC)=1 + IF(ISTRAN.EQ.2)ISENSE(IMATC)=-1 + WRITE(KBOUT,1000)LLINO(IMATC),IPOSC(IMATC),ISTRAN, + + IPOSG(IMATC) + 1000 FORMAT + + (' Contig',I5,' position',I6,' matches strand',I2, + + ' at position',I5) + ELSE + CALL ERROM(KBOUT,'Warning: too many overlaps') + END IF + END + SUBROUTINE ADISM4(IDIM,IDIMG,SAVPS,SAVPG,IDSAV, + +CENDS,NENDS,IDCEND,MAXCON,ILEFTS,ILC,IPOSC,IPOSG,ISENSE, + +LLINO,IMATC,ISTRAN,MAXC,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER ILEFTS(MAXC),ILC(MAXC),IPOSC(MAXC),IPOSG(MAXC) + INTEGER ISENSE(MAXC),LLINO(MAXC) + INTEGER CENDS(MAXCON) + INTEGER NENDS(MAXCON) + INTEGER SAVPS(IDSAV),SAVPG(IDSAV) + NEXTC=IDIM+1 + CALL BUB2AS(SAVPS,SAVPG,IDSAV) + IMATC=IMATC+1 + CALL ADISM3(SAVPS(1),SAVPG(1),CENDS,NENDS,IDCEND,MAXCON, + + ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN,NEXTC,MAXC, + + KBOUT) + LEND=IDIMG-SAVPG(1)+SAVPS(1) + DO 10 I=2,IDSAV + IF((SAVPS(I).LT.LEND).AND.(SAVPS(I).LT.NEXTC))GO TO 10 + IMATC=IMATC+1 + CALL ADISM3(SAVPS(I),SAVPG(I),CENDS,NENDS,IDCEND,MAXCON, + + ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN,NEXTC,MAXC, + + KBOUT) + LEND=IDIMG-SAVPG(I)+SAVPS(I) +10 CONTINUE + IMATC = MIN(IMATC,MAXC) + RETURN + END + SUBROUTINE AEDIT(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LGEL,NCONT, + +GEL,MAXGEL,CON,IDC,IDEVW,IDEVR,LREG,RREG,KBOUT) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),CON(IDC) + INTEGER RREG,PC,PCA,PG + NG = LGEL + PG = RELPG(NG) + PC = LREG + NTT = 0 + NCT = 0 + NDT = 0 +10 CONTINUE +C WRITE(*,*)'GEL',NG + CALL READW(IDEVW,NG,GEL,MAXGEL) + LG = ABS(LNGTHG(NG)) + IF(PC.LT.LREG) PC = LREG + PCA = PC - LREG + 1 + IG = PC - PG + 1 + LC = MIN(LG,RREG-PC+1) +C WRITE(*,*)'PC,PG,IG,LG,PCA,LC',PC,PG,IG,LG,PCA,LC + CALL ET(GEL(IG),LG,CON(PCA),LC,NE) + NTT = NTT + NE + CALL EC(GEL(IG),LG,CON(PCA),LC,NE) + NCT = NCT + NE + CALL ED(GEL(IG),LG,CON(PCA),LC,ND) + NDT = NDT + ND + CALL WRITEW(IDEVW,NG,GEL,MAXGEL) + IF(ND.GT.0) THEN + K = LNGTHG(NG) + LNGTHG(NG) = ABS(LNGTHG(NG)) - ND + LNGTHG(NG) = SIGN(LNGTHG(NG),K) + CALL WRITER(IDEVR,NG,RELPG(NG),LNGTHG(NG),LNBR(NG),RNBR(NG)) + END IF + IF(RNBR(NG).NE.0) THEN + NG = RNBR(NG) + PG = RELPG(NG) + PC = PG + IF(PG.LE.RREG) GO TO 10 + END IF + CALL EDR(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LGEL,NCONT, + +CON,IDC,IDEVW,IDEVR,LREG) + WRITE(KBOUT,1000)NTT +1000 FORMAT(' Number of transpositions=',I6) + WRITE(KBOUT,1001)NCT +1001 FORMAT(' Number of changes =',I6) + WRITE(KBOUT,1002)NDT +1002 FORMAT(' Number of deletions =',I6) + END +C AJOIN2 +C COMPLETES JOIN AND RETURNS LENGTH OF NEW CONTIG IN LLINOR + SUBROUTINE AJOIN2(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +RELX,LLINOL,LLINOR,LNCONL,LNCONR,IDEVR) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNBR(IDBSIZ),RNBR(IDBSIZ),LNGTHG(IDBSIZ) + INTEGER RELX +C RELX IS THE POSITION OF THE JOINT +C LLINOL IS THE LEFT GEL NUMBER OF THE LEFT CONTIG +C LLINOR IS THE LEFT GEL OF THE RIGHT CONTIG +C LNCONL IS THE LEFT CONTIG LINE NUMBER +C LNCONR IS THE RIGHT CONTIG LINE NUMBER +C +C ADJUST ALL RELATIVE POSITIONS IN RIGHT CONTIG + N=LLINOR + RELPG(N)=RELX +50 CONTINUE + IF(RNBR(N).EQ.0)GO TO 60 + N=RNBR(N) + RELPG(N)=RELPG(N)+RELX-1 + GO TO 50 +60 CONTINUE +C +C FIX UP NEW GEL LINE FOR OLD LEFT OF RIGHT CONTIG + LNBR(LLINOR)=RNBR(LNCONL) +C FIX UP RIGHT GEL OF LEFT CONTIG + N=RNBR(LNCONL) + RNBR(N)=LLINOR +C MERGE WILL SORT OUT THE CORRECT NEIGHBOURS +C + CALL MERGE(RELPG,LNGTHG,LNBR,RNBR,LNCONL,IDBSIZ) +C MERGE DOES NOT WRITE TO DISK + N=LNBR(LNCONL) +65 CONTINUE +C WRITE(IDEVR,REC=N)RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N),LNBR(N),RNBR(N)) + N=RNBR(N) + IF(N.NE.0)GO TO 65 +C CONTIG LINES + X=RELPG(LNCONR)+RELX-1 +C LENGTH MAY NOT HAVE INCREASED! + IF(X.GT.RELPG(LNCONL))RELPG(LNCONL)=X +C SAVE LENGTH OF NEW CONTIG + RELX=RELPG(LNCONL) +C WRITE(IDEVR,REC=LNCONL)RELPG(LNCONL),LNGTHG(LNCONL),LNBR(LNCONL), +C 1RNBR(LNCONL) + CALL WRITER(IDEVR,LNCONL,RELPG(LNCONL),LNGTHG(LNCONL), + +LNBR(LNCONL),RNBR(LNCONL)) +C +C NOW MOVE ALL DATA DOWN TO DELETE OLD RIGHT END + N=IDBSIZ-NCONTS + M=LNCONR-N + IF(M.EQ.0)GO TO 80 + K=LNCONR + J=LNCONR-1 + DO 70 I=1,M + RELPG(K)=RELPG(J) + LNGTHG(K)=LNGTHG(J) + LNBR(K)=LNBR(J) + RNBR(K)=RNBR(J) +C WRITE(IDEVR,REC=K)RELPG(K),LNGTHG(K),LNBR(K),RNBR(K) + CALL WRITER(IDEVR,K,RELPG(K),LNGTHG(K),LNBR(K),RNBR(K)) + K=K-1 + J=J-1 +70 CONTINUE +80 CONTINUE + NCONTS=NCONTS-1 +C WRITE(IDEVR,REC=IDBSIZ)NGELS,NCONTS + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) +90 CONTINUE + RETURN + END +C SUBROUTINE AJOIN3 + SUBROUTINE AJOIN3(RELPG,IDBSIZ,LINCON,ITYPE,ISENSE,JOINT,IDIM22, + +KLASS,IOVER,KBOUT,PL,PR) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),LINCON(2),IDIM22(2) + INTEGER ITYPE(2),ISENSE(2),JOINT(2),PL(2),PR(2) +C +C CALC POSITIONS OF CONTIGS RELATIVE TO FIXED GEL + DO 20 I=1,2 +C R+ + IF((ITYPE(I).NE.-1).OR.(ISENSE(I).NE.1))GO TO 11 + PL(I)=-1*JOINT(I)+2 + PR(I)=PL(I)+RELPG(LINCON(I))-1 + GO TO 20 +C L+ +11 CONTINUE + IF((ITYPE(I).NE.1).OR.(ISENSE(I).NE.1))GO TO 12 + PL(I)=JOINT(I) + PR(I)=PL(I)+RELPG(LINCON(I))-1 + GO TO 20 +C R- +12 CONTINUE + IF((ITYPE(I).NE.-1).OR.(ISENSE(I).NE.-1))GO TO 13 + PR(I)=JOINT(I)+IDIM22(I)-1 + PL(I)=PR(I)-RELPG(LINCON(I))+1 + GO TO 20 +C L- +13 CONTINUE + PR(I)=IDIM22(I)-JOINT(I)+1 + PL(I)=PR(I)-RELPG(LINCON(I))+1 +20 CONTINUE +C LENGTH OF OVERLAP + IOVER=MIN(PR(1),PR(2))-MAX(PL(1),PL(2))+1 + WRITE(KBOUT,1002)IOVER +1002 FORMAT(' Length of overlap between the contigs=',I6) +C +C CLASS NUMBER 1-16 + KLASS=1 + IF(ITYPE(1).EQ.1)KLASS=KLASS+8 + IF(ISENSE(1).EQ.-1)KLASS=KLASS+4 + IF(ITYPE(2).EQ.1)KLASS=KLASS+2 + IF(ISENSE(2).EQ.-1)KLASS=KLASS+1 +C WRITE(KBOUT,1001)KLASS +C1001 FORMAT(' CLASS OF JOIN=',I6) + RETURN + END +C ALINE +C +C ROUTINE TO LINE UP 2 SEQS. +C IT SLIDES,REMOVES OVERLAPPING MATCHES, +C SORTS MATCHES INTO ASCENDING ORDER, THEN DOES DOES A TOPOLOGICAL +C CHECK, AND THEN PRODUCES 2 LINED UP SEQS WITH PADDING CHARS +C VARIABLES +C SEQ1 CONSENSUS +C SEQ2 GEL ORIGINAL IN CORRECT ORIENTATION +C SEQG2 ALIGNED GEL +C SEQC2 ALIGNED CONSENSUS +C SEQ3 SAVED GEL RAW DATA +C ISAV1,2,3 STORE MATCHES AND POSITIONS +C IDSAV NUMBER ISAV'S +C IDC LENGTH OF INPUT SEQ1 +C IDIM2 LENGTH OF INPUT SEQ2 +C IDOUT LENGTH OF OUTPUT ALIGNED SEQ1 +C IDIM2 LENGTH OF SEQ2 ON OUTPUT AFTER ALIGNMENT +C MINSLI MIN MATCH FOR SLIDING +C IFAIL FLAG TO SHOW IF ALIGNMENT FAILED DUE TO TOO +C MANY MISMATCHES OR TOPOLIGICAL CHECK OR TOO MANY OR TOO MANY +C PADDING CHARS. 1=FAIL,0=PASS +C + SUBROUTINE ALINE(SEQ1,SEQ2,SEQG2,SEQC2,ISAV1,ISAV2,ISAV3, + +IDSAV,IDC,IDIM2,IDOUT,IC1,IG1,MINSLI,JOINT, + +ITOTPC,ITOTPG,IFAIL,ITYPE,MAXPC,MAXPG,PERMAX,KBOUT,SEQ3,MAXGEL, + +PERCM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDC),SEQ2(IDIM2),SEQG2(IDOUT),SEQC2(IDOUT) + CHARACTER SEQ3(MAXGEL) + INTEGER ISAV1(IDSAV),ISAV2(IDSAV),ISAV3(IDSAV) + MINSLT=MINSLI +C SAVE SEQ2 + CALL SQCOPY(SEQ2,SEQ3,IDIM2) + CALL MSTLKL(SEQ3,IDIM2) + IFAIL=1 +C FIND MATCHES + IPP=IDSAV + CALL SLIDER(SEQ1,IDC,SEQ3,IDIM2,IC1,IG1,MAXPG,MAXPC,MINSLT, + +ISAV1,ISAV2,ISAV3,IPP) + IF(IPP.GT.IDSAV)RETURN + IF(IPP.LT.1)RETURN + CALL REMOVL(ISAV2,ISAV3,ISAV1,IPP) + CALL BUB3AS(ISAV2,ISAV3,ISAV1,IPP) +C DO TOPOLOGICAL CHECK + CALL TPCHEK(ISAV2,ISAV3,ISAV1,IPP) + IFAIL=0 + CALL LINEUP(SEQ2,SEQ1,SEQG2,SEQC2,IDC,IDIM2,IDOUT,ISAV3,ISAV2, + +ISAV1,IPP,ITOTPC,ITOTPG,JOINT,ITYPE,KBOUT,MAXGEL,IFAIL) + IF(IFAIL.NE.0)RETURN +C IDIM2 IS NOW LENGTH OF ALIGNED GEL + WRITE(KBOUT,1052)ITOTPC,ITOTPG +1052 FORMAT(' Padding in contig= ',I4,' and in gel= ',I4) + CALL DALIGN(SEQC2,SEQG2,SEQ3,MAXGEL,IDOUT,IDIM2,JOINT, + +ITYPE,PERCM,KBOUT,IFAIL) +C ARE ALL CHECKS OK? + IF(ITOTPC.GT.MAXPC)IFAIL=1 + IF(ITOTPG.GT.MAXPG)IFAIL=1 + IF(PERCM.GT.PERMAX)IFAIL=1 + END + SUBROUTINE ARCSER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN +C SEARCHES FOR ARCHIVE NAMES + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER NAME1*10,NAME2*10 +10 CONTINUE + L = 0 + CALL GTSTR('Archive name',' ',NAME1,L,KBOUT,KBIN,INFLAG) + IF(L.EQ.0) RETURN + CALL CCASE(NAME1,1) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.3) RETURN + IF(NAME1(1:1).EQ.' ') RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + DO 100 I=1,NGELS + CALL READN(IDEVN,I,NAME2) + IF(NAME1.EQ.NAME2) THEN + WRITE(KBOUT,1003)NAME2,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) +1003 FORMAT(' ',A,2X,I4,2X,I6,2X,I6,2X,I6,2X,I6/) + GO TO 10 + END IF +100 CONTINUE + WRITE(KBOUT,1004)NAME1 +1004 FORMAT(' ',A,' Not in database') + GO TO 10 + END + SUBROUTINE AUTOCN(SEQ1,IDIM,GEL,IDIMG,ILEFTS,ILC,IPOSC, + +IPOSG,ISENSE,LLINO,IMATC,IFCOMP,MINMAT,POSNS,WORDP,WORDN, + +CONST,LENGTH,LPOWRC,KBOUT,MATCH,MAXGEL,MAXGLM,GELCOP,GELN, + +SAVPS,SAVPG,SAVL,MAXSAV,CENDS,NENDS,MAXCON, + +SEQG2,SEQC2,SEQ4,IDOUT,IDIM22,ITOTPG,ITOTPC,JOINT,IFAIL, + +ITYPE,MAXPC,MAXPG,PERMAX,MINSLI,SEQG3,SEQC3,KFAIL) +C AUTHOR: RODGER STADEN +C changed 29-11-90 to make first in list of alignments the best + INTEGER ILEFTS(2),ILC(2),IPOSC(2),IPOSG(2),ISENSE(2),LLINO(2) + INTEGER POSNS(IDIM),GELN(MAXGLM),WORDP(LPOWRC),SAVPS(MAXSAV) + INTEGER SAVPG(MAXSAV),SAVL(MAXSAV) + INTEGER WORDN(LPOWRC) + CHARACTER GELCOP(MAXGLM),MATCH(MAXGLM) + INTEGER CENDS(MAXCON),NENDS(MAXCON) + INTEGER CONST(LENGTH) + CHARACTER SEQ1(IDIM),GEL(MAXGLM) +C + CHARACTER SEQG2(MAXGLM,2),SEQC2(MAXGLM,2),SEQ4(MAXGLM) + INTEGER IDOUT(2),IDIM22(2),ITOTPG(2),ITOTPC(2),JOINT(2) + INTEGER IFAIL(2),ITYPE(2) + PARAMETER (MAXC = 10) + CHARACTER SEQG3(MAXGLM),SEQC3(MAXGLM) + INTEGER JLEFTS(MAXC),JLC(MAXC),JPOSC(MAXC),JPOSG(MAXC) + INTEGER JSENSE(MAXC),JLLINO(MAXC) + REAL PERMIS(2) + IFAIL(1) = 1 + IFAIL(2) = 1 + KFAIL = 0 +C 23-8-90 Need to deal with failures in a better way. Problem is +C case where overlaps are found but fail to align. In future +C signal them with new variable KFAIL which will be nonzero +C if any alignment fails. +C 29-11-90 Changed sorting of overlaps so that the best is first in the +C list returned to caller. +C SAVE GEL + CALL SQCOPY(GEL,GELCOP,IDIMG) +C COUNT NUMBER OF CONTIGS THAT MATCH + IMATC=0 + IDCEND=MAXCON + CALL BUSY(KBOUT) + CALL FNDCON(SEQ1,IDIM,CENDS,NENDS,IDCEND,MAXCON,KBOUT) + CALL ENCO(SEQ1,IDIM,POSNS,CONST,LENGTH) + CALL ENCONA(POSNS,IDIM,WORDP,WORDN,LPOWRC,LENGTH) +1 CONTINUE + ISTRAN=1 +2 CONTINUE + CALL MSTLKL(GEL,IDIMG) + CALL ENCO(GEL,IDIMG,GELN,CONST,LENGTH) + IDSAV=MAXSAV + CALL CFGEL(GELN,IDIMG,POSNS,IDIM,WORDP,WORDN,LENGTH,LPOWRC, + +SAVPG,SAVPS,SAVL, + +IDSAV,SEQ1,GEL,MINMAT,IFCOMP,KBOUT) + IF(IFCOMP.NE.0)RETURN + IF(IDSAV.NE.0)THEN + CALL ADISM4(IDIM,IDIMG,SAVPS,SAVPG,IDSAV,CENDS,NENDS, + + IDCEND,MAXCON,JLEFTS,JLC,JPOSC,JPOSG,JSENSE,JLLINO, + + IMATC,ISTRAN,MAXC,KBOUT) + END IF + ISTRAN=ISTRAN+1 + IF(ISTRAN.EQ.2) THEN + CALL SQCOPY(GELCOP,GEL,IDIMG) + CALL SQREV(GEL,IDIMG) + CALL SQCOM(GEL,IDIMG) + GO TO 2 + END IF + CALL SQCOPY(GELCOP,GEL,IDIMG) + KSENSE = 0 + WRITE(KBOUT,*)'Total matches found',IMATC + IF(IMATC.EQ.0) THEN + IFAIL(1) = 0 + RETURN + END IF + JMATC = 0 + DO 100 I = 1,IMATC + IF(JSENSE(I).EQ.-1) THEN + IF(KSENSE.EQ.0) THEN + CALL SQREV(GEL,IDIMG) + CALL SQCOM(GEL,IDIMG) + KSENSE = 1 + END IF + END IF + JDIM22 = IDIMG + JDOUT = MAXGEL + IDSAV = MAXSAV + WRITE(KBOUT,*)'Trying to align with contig',JLLINO(I) + CALL ALINE(SEQ1(JLEFTS(I)),GEL,SEQG3,SEQC3, + + SAVPS,SAVPG,SAVL,IDSAV,JLC(I),JDIM22,JDOUT, + + JPOSC(I),JPOSG(I),MINSLI,JJOINT,JTOTPC,JTOTPG, + + JFAIL,JTYPE,MAXPC,MAXPG,PERMAX,KBOUT,SEQ4,MAXGEL,PERMS) + IF(JFAIL.EQ.0) THEN + JMATC = JMATC + 1 + IF(JMATC.EQ.1) THEN +C Save in elements 1 + CALL COPYM(JLEFTS(I),ILEFTS(1),JLC(I),ILC(1), + + JPOSC(I),IPOSC(1),JSENSE(I),ISENSE(1), + + JLLINO(I),LLINO(1),JJOINT,JOINT(1),JTOTPC, + + ITOTPC(1),JTOTPG,ITOTPG(1),JTYPE,ITYPE(1), + + JDOUT,IDOUT(1),JDIM22,IDIM22(1), + + SEQG3,SEQG2(1,1),SEQC3,SEQC2(1,1), + + PERMS,PERMIS(1)) + IFAIL(1) = 0 + ELSE IF(JMATC.EQ.2) THEN + IF(PERMS.LT.PERMIS(1)) THEN +C Better match so save in elements 1, so copy 1 to 2 first + CALL COPYM(ILEFTS(1),ILEFTS(2),ILC(1),ILC(2), + + IPOSC(1),IPOSC(2),ISENSE(1),ISENSE(2), + + LLINO(1),LLINO(2),JOINT(1),JOINT(2),ITOTPC(1), + + ITOTPC(2),ITOTPG(1),ITOTPG(2),ITYPE(1),ITYPE(2), + + IDOUT(1),IDOUT(2),IDIM22(1),IDIM22(2), + + SEQG2(1,1),SEQG2(1,2),SEQC2(1,1),SEQC2(1,2), + + PERMIS(1),PERMIS(2)) + IFAIL(2) = 0 +C Now save in 1 + CALL COPYM(JLEFTS(I),ILEFTS(1),JLC(I),ILC(1), + + JPOSC(I),IPOSC(1),JSENSE(I),ISENSE(1), + + JLLINO(I),LLINO(1),JJOINT,JOINT(1),JTOTPC, + + ITOTPC(1),JTOTPG,ITOTPG(1),JTYPE,ITYPE(1), + + JDOUT,IDOUT(1),JDIM22,IDIM22(1), + + SEQG3,SEQG2(1,1),SEQC3,SEQC2(1,1), + + PERMS,PERMIS(1)) + ELSE +C Save in element 2 + CALL COPYM(JLEFTS(I),ILEFTS(2),JLC(I),ILC(2), + + JPOSC(I),IPOSC(2),JSENSE(I),ISENSE(2), + + JLLINO(I),LLINO(2),JJOINT,JOINT(2),JTOTPC, + + ITOTPC(2),JTOTPG,ITOTPG(2),JTYPE,ITYPE(2), + + JDOUT,IDOUT(2),JDIM22,IDIM22(2), + + SEQG3,SEQG2(1,2),SEQC3,SEQC2(1,2), + + PERMS,PERMIS(2)) + IFAIL(2) = 0 + END IF + ELSE + IF(PERMS.LT.PERMIS(1)) THEN +C Better match so save in elements 1, so copy 1 to 2 first + CALL COPYM(ILEFTS(1),ILEFTS(2),ILC(1),ILC(2), + + IPOSC(1),IPOSC(2),ISENSE(1),ISENSE(2), + + LLINO(1),LLINO(2),JOINT(1),JOINT(2),ITOTPC(1), + + ITOTPC(2),ITOTPG(1),ITOTPG(2),ITYPE(1),ITYPE(2), + + IDOUT(1),IDOUT(2),IDIM22(1),IDIM22(2), + + SEQG2(1,1),SEQG2(1,2),SEQC2(1,1),SEQC2(1,2), + + PERMIS(1),PERMIS(2)) + IFAIL(2) = 0 +C Now save in 1 + CALL COPYM(JLEFTS(I),ILEFTS(1),JLC(I),ILC(1), + + JPOSC(I),IPOSC(1),JSENSE(I),ISENSE(1), + + JLLINO(I),LLINO(1),JJOINT,JOINT(1),JTOTPC, + + ITOTPC(1),JTOTPG,ITOTPG(1),JTYPE,ITYPE(1), + + JDOUT,IDOUT(1),JDIM22,IDIM22(1), + + SEQG3,SEQG2(1,1),SEQC3,SEQC2(1,1), + + PERMS,PERMIS(1)) + ELSE IF(PERMS.LT.PERMIS(2)) THEN +C Save in element 2 + CALL COPYM(JLEFTS(I),ILEFTS(2),JLC(I),ILC(2), + + JPOSC(I),IPOSC(2),JSENSE(I),ISENSE(2), + + JLLINO(I),LLINO(2),JJOINT,JOINT(2),JTOTPC, + + ITOTPC(2),JTOTPG,ITOTPG(2),JTYPE,ITYPE(2), + + JDOUT,IDOUT(2),JDIM22,IDIM22(2), + + SEQG3,SEQG2(1,2),SEQC3,SEQC2(1,2), + + PERMS,PERMIS(2)) + END IF + END IF + ELSE + KFAIL = 1 + END IF +100 CONTINUE + IMATC = MIN(2,JMATC) + END + SUBROUTINE BATIN(SEQ,MAXSEQ,KBIN,KBOUT,IDEV1,IDEV2,IDEV3, + +FILNAM,IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR RODGER STADEN + CHARACTER SEQ(MAXSEQ) + CHARACTER FILNAM*(*),HELPF*(*) + CHARACTER BASE(4),BKEY(4) + SAVE BASE,BKEY + DATA BASE/'A','C','G','T'/ + DATA BKEY/'N','M',',','.'/ + CALL CBASE(BASE,BKEY,IOK,ICBASE, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + FILNAM = ' ' + CALL OPENF1(IDEV1,FILNAM,1,IOK,KBIN,KBOUT, + +'File of file names', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + NGELS=0 +10 CONTINUE + IDIM=MAXSEQ + WRITE(KBOUT,1003) +1003 FORMAT(' Type data on lines of < 80 characters, finish with @'/) + CALL ARRFIM(KBIN,SEQ,IDIM,KBOUT) + IF(IDIM.GT.0)THEN + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,1,IOK,KBIN,KBOUT, + + 'File name for gel reading', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.EQ.0) THEN + IF(ICBASE.EQ.0) THEN + CALL CCASEA(SEQ,IDIM,2) + DO 5 J = 1,4 + CALL SUBS(SEQ,IDIM,BKEY(J),BASE(J)) +5 CONTINUE + END IF + CALL CCASEA(SEQ,IDIM,1) + CALL FMTDKN(IDEV2,SEQ,IDIM) + CLOSE(UNIT=IDEV2) + NGELS=NGELS+1 + WRITE(IDEV1,1002)FILNAM +1002 FORMAT( A) + END IF + END IF + CALL YESNO(IANS,'Type in another gel reading', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IANS.EQ.0) GO TO 10 + CALL YESNO(IANS,'List gel readings', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IANS.EQ.0) THEN + REWIND IDEV1 + DO 50 I=1,NGELS + IDIM=MAXSEQ + READ(IDEV1,1002)FILNAM + CALL OPENRS(IDEV2,FILNAM,IOK,LRECL,2) + IF(IOK.NE.0)THEN + WRITE(KBOUT,*)' Error opening file' + GO TO 50 + END IF + WRITE(IDEV3,1007)FILNAM +1007 FORMAT(' ',A) + CALL ARRFIM(IDEV2,SEQ,IDIM,KBOUT) + IF(IDIM.GT.0)CALL FMTDB(SEQ,IDIM,1,IDIM,60,IDEV3) + CLOSE(UNIT=IDEV2) +50 CONTINUE + END IF + CLOSE(UNIT=IDEV1) + WRITE(KBOUT,*)'Number of gel readings entered',NGELS + END +C BEDIN +C +C SUBROUTINE TO BED A NEW GEL INTO THE DATABASE. IT DISPLAYS +C THE JOIN AND ALLOWS CHANGES TO IT, THEN IT ALLOWS EDITING OF +C THE NEW GEL USING EDITG OR THE GELS IN THE DATABASE USING +C DBEDIT. ANY CHANGES CAN BE DISPLAYED USING DSPLAY. THIS SUBROUTINE +C RETURNS NOPT TO TELL OF THE OUTCOME OF ITS USE:3=OK,1=REJOIN,4=GIVE UP +C ON INPUT NOPT=2 MEANS LEFT END OVERLAP + SUBROUTINE BEDIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,GEL2,LINCON,LLINO,NOPT,X,IDBSIZ,IDEV,KBIN,KBOUT, + +IDEVR,IDEVW,IDEVN,LINLEN,PERCD, + +HELPS1,HELPE1,HELPS2,HELPE2,FILEH,IDEVH,MAXGEL,IDM) + CHARACTER FILEH*(*) + INTEGER HELPS1,HELPE1,HELPS2,HELPE2 +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),ANS + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + CHARACTER GEL2(MAXGEL) + PARAMETER (MAXPRM = 32) + CHARACTER PROMPT(5)*(MAXPRM) + INTEGER LREG,RIGHTM + INTEGER X,RREG +C SAVE NOPT FOR LEFT END OR RIGHT JOIN + NTYPE=NOPT + LREG=X + IF(NTYPE .EQ.2)LREG=1 + RREG=LREG+49 + IF(RREG.GT.RELPG(LINCON))RREG=RELPG(LINCON) + I1=1 + IF(NTYPE.EQ.2)I1=X + I2=I1+49 + IF(I2.GT.ABS(LNGTHG(NGELS)))I2=ABS(LNGTHG(NGELS)) + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR,GEL2, + +LLINO,LINCON,LREG,RREG,GEL,I1,I2,2,LLINOR,IDBSIZ,IDEV, + +KBOUT,IDEVW,IDEVN,LINLEN,PERCD,MAXGEL,IDM) + CALL YESNO(ANS,'Joint correct', + +HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.NE.0) THEN + NOPT = 1 + RETURN + END IF +20 CONTINUE + CALL BELL(1,KBOUT) +C DBMENU now defunct for sap - so we use RADION instead +C CALL DBMENU(2,JOPT,HELPS1,HELPE1,FILEH,IDEVH, +C +KBIN,KBOUT) + PROMPT(1) = 'Complete entry' + PROMPT(2) = 'Edit contig' + PROMPT(3) = 'Display' + PROMPT(4) = 'Edit new gel reading' + JOPT = 1 + CALL RADION('Enter options', PROMPT, 4, JOPT, IHELPS, + + IHELPE, FILEH, IDEVH, KBIN, KBOUT) +C GIVE UP + IF(JOPT.EQ.-1)THEN + CALL YESNO(ANS,'Quit', + + HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.EQ.0) THEN + NOPT = 4 + RETURN + END IF + END IF +C COMPLETE JOIN + IF(JOPT.EQ.1)THEN + CALL YESNO(ANS,'Complete entry', + + HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.NE.0) GO TO 20 + NOPT=3 + RETURN + END IF +100 CONTINUE + IF(JOPT.EQ.4)THEN +C EDIT NEW GEL + IDIM=ABS(LNGTHG(NGELS)) + CALL EDITGL(GEL,GEL2,IDIM,KBIN,KBOUT,MAXGEL, + + HELPS2,HELPE2,FILEH,IDEVH,1) + LNGTHG(NGELS)=SIGN(IDIM,LNGTHG(NGELS)) + END IF +200 CONTINUE + IF(JOPT.EQ.3)THEN +C DISPLAY + LLINO=LNBR(LINCON) + LEFTMN=X + RIGHTM=X+ABS(LNGTHG(NGELS))-1 + RIGHTM=MIN(RIGHTM,RELPG(LINCON)) + IF(NTYPE.EQ.2)THEN + LEFTMN=1 + RIGHTM=ABS(LNGTHG(NGELS))-X+1 + RIGHTM=MIN(RIGHTM,RELPG(LINCON)) + END IF + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LEFTMN, + + RIGHTM,LREG, + + RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + HELPS1,HELPE1,FILEH,IDEVH,IERR) + IF(IERR.NE.0) GO TO 20 + IF(NTYPE.EQ.2)GO TO 210 + I1=LREG-X+1 + IF(I1.GT.ABS(LNGTHG(NGELS)))GO TO 200 + I2=RREG-X+1 + IF(I2.GT.ABS(LNGTHG(NGELS)))GO TO 200 + GO TO 230 +210 CONTINUE + I1=X+LREG-1 + I2=X+RREG-1 + IF(I1.GT.ABS(LNGTHG(NGELS)))GO TO 200 + IF(I2.GT.ABS(LNGTHG(NGELS)))GO TO 200 +230 CONTINUE + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR,GEL2, + + LLINO,LINCON,LREG,RREG,GEL,I1,I2,2,LLINOR,IDBSIZ,IDEV, + + KBOUT,IDEVW,IDEVN,LINLEN,PERCD,MAXGEL,IDM) + LLINO=LNBR(LINCON) + END IF +300 CONTINUE + IF(JOPT.EQ.2)THEN +C EDIT DATABASE + IF(NTYPE.EQ.2)THEN + IMAXL=1 + IMAXR=ABS(LNGTHG(NGELS))-X+1 + ELSE IF(NTYPE.EQ.1)THEN + IMAXL=X + IMAXR=X+ABS(LNGTHG(NGELS))-1 + END IF + CALL DBEDIT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,GEL2, + + LINCON,IMAXL,IMAXR,IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW, + + HELPS2,HELPE2,FILEH,IDEVH,MAXGEL) + END IF + IF(JOPT.EQ.1)THEN + CALL HELP2(HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + END IF + GO TO 20 + END + SUBROUTINE BREAKC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW,IDEVN, + +IHELPS,IHELPE,IHELP1,IHELP2,FILEH,IDEVH,IOK) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER CLEN,CHAINL,GCLIN + EXTERNAL CLEN,CHAINL,GCLIN +C ROUTINE TO BREAK A CONTIG INTO 2 +C LEFT GEL OF NEW RIGHT CONTIG IS IR +C RIGHT GEL OF NEW LEFT CONTIG IS IL +C LEFT GEL OF OLD LEFT CONTIG IS ILO +C CONTIG LINE OF OLD CONTIG IS NCONTO +C CONTIG LINE OF NEW RIGHT CONTIG IS NCONTR +C CONTIG LINE OF NEW LEFT CONTIG IS NCONTO +C LENGTH OF OLD CONTIG IS LCONTO + IOK = 1 + NCONTR = IDBSIZ - NCONTS - 1 + IF(NCONTR.LE.NGELS) THEN + WRITE(KBOUT,*)'Insufficient space for new contig line.' + WRITE(KBOUT,*)'Increase database size with copy' + RETURN + END IF +10 CONTINUE + MN = 0 + MX = NGELS + IR = 0 + CALL GETINT(MN,MX,IR, + + 'Number of gel reading that will become a left end', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF(IVAL.LT.1) RETURN + IR = IVAL + IL = LNBR(IR) + IF(IL.EQ.0)THEN + WRITE(KBOUT,*)'Gel number',IR,' is already a left end' + GO TO 10 + END IF + ILO = CHAINL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,IR) + IF(ILO.EQ.0)THEN + WRITE(KBOUT,*) + +'Problem with this contig. Check logical consistency' + WRITE(KBOUT,*)'of database. Break not made' + RETURN + END IF + NCONTO = GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,ILO) + IF(NCONTO.EQ.0)THEN + WRITE(KBOUT,*)'No contig line for this contig. Check logical' + WRITE(KBOUT,*)'consistency of database. Break not made' + RETURN + END IF + LCONTO = RELPG(NCONTO) + IF(LCONTO.LT.1)THEN + WRITE(KBOUT,*)'Contig has zero length. Break not made' + RETURN + END IF + CALL CBREAK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,KBOUT,IDEVR,IDEVW,IDEVN,IR,IL,ILO,NCONTO,NCONTR,IOK) + END + SUBROUTINE CBREAK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,KBOUT,IDEVR,IDEVW,IDEVN,IR,IL,ILO,NCONTO,NCONTR,IOK) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER CLEN + EXTERNAL CLEN +C ROUTINE TO BREAK A CONTIG INTO 2 +C LEFT GEL OF NEW RIGHT CONTIG IS IR +C RIGHT GEL OF NEW LEFT CONTIG IS IL +C LEFT GEL OF OLD LEFT CONTIG IS ILO +C CONTIG LINE OF OLD CONTIG IS NCONTO +C CONTIG LINE OF NEW RIGHT CONTIG IS NCONTR +C CONTIG LINE OF NEW LEFT CONTIG IS NCONTO +C LENGTH OF OLD CONTIG IS LCONTO + IOK = 1 + NCONTS = NCONTS + 1 +C WRITE LAST LINE OF DB + WRITE(KBOUT,*)'Increasing number of contigs by 1' + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) +C MAKE NEW CONTIG A COPY OF OLD + RELPG(NCONTR) = RELPG(NCONTO) + LNGTHG(NCONTR) = LNGTHG(NCONTO) + LNBR(NCONTR) = IR + RNBR(NCONTR) = RNBR(NCONTO) + WRITE(KBOUT,*)'Writing new right contig line' + CALL WRITER(IDEVR,NCONTR,RELPG(NCONTR),LNGTHG(NCONTR), + +LNBR(NCONTR),RNBR(NCONTR)) +C NEED LENGTH FOR OLD LEFT CONTIG + RNBR(IL) = 0 + L = CLEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,ILO) + IF(L.LT.1)THEN + WRITE(KBOUT,*) + + 'New left contig has zero length. Break not made' + RETURN + END IF + RELPG(NCONTO) = L + RNBR(NCONTO) = IL +C DO CONTIG LINE FOR NEW LEFT CONTIG + WRITE(KBOUT,*)'Writing new left contig line' + WRITE(KBOUT,*)'New length=',RELPG(NCONTO) + WRITE(KBOUT,*)'New right gel=',RNBR(NCONTO) + CALL WRITER(IDEVR,NCONTO,RELPG(NCONTO),LNGTHG(NCONTO), + +LNBR(NCONTO),RNBR(NCONTO)) +C DO GEL LINE FOR RIGHT GEL OF NEW LEFT CONTIG + WRITE(KBOUT,*)'Writing new right gel of left contig' + WRITE(KBOUT,*)'Gel number=',IL + CALL WRITER(IDEVR,IL,RELPG(IL),LNGTHG(IL), + +LNBR(IL),RNBR(IL)) +C DO GEL LINE FOR NEW RIGHT CONTIG + LNBR(IR) = 0 + WRITE(KBOUT,*)'Writing new left gel of right contig' + WRITE(KBOUT,*)'Gel number=',IR + CALL WRITER(IDEVR,IR,RELPG(IR),LNGTHG(IR), + +LNBR(IR),RNBR(IR)) +C NOW SHIFT + I = 1 - RELPG(IR) + WRITE(KBOUT,*)'Shifting gels in right contig by distance=',I + CALL SHIFTC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDEVR, + +IDBSIZ,IR,NCONTR,I) + WRITE(KBOUT,*)'Right contig length=',RELPG(NCONTR) + WRITE(KBOUT,*)'Break completed' + IOK = 0 + END +C BUBBL3 +C SUBROUTINE TO SORT INTEGER ARRAY (LIST) INTO ASCENDING ORDER +C + SUBROUTINE BUBBL3(LIST,LISTEL,LISTAL,IDIM) +C AUTHOR: RODGER STADEN + INTEGER LIST(IDIM),LISTEL(IDIM),LISTAL(IDIM) +C +C SET POINTERS TO ZERO + I=0 + J=0 +C +10 CONTINUE +C +C SET I=J IF WE HAVE JUST CORRECTLY POSITIONED AN ELEMENT + IF(J.GT.I)I=J +C +C INCREMENT POINTER TO NEXT ELEMENT + I=I+1 +C TEST FOR END OF ARRAY + IF(I.EQ.IDIM)RETURN +C +20 CONTINUE +C +C COMPARE ADJACENT ELEMENTS + IF(LIST(I).GE.LIST(I+1))GO TO 10 +C +C FIRST MOVE THIS ELEMENT? IF SO SET POINTER TO ITS INITIAL POSITION + IF(J.LT.I)J=I +C +C EXCHANGE ADJACENT ELEMENTS + ITEMP=LIST(I) + LIST(I)=LIST(I+1) + LIST(I+1)=ITEMP +C + ITEMP=LISTEL(I) + LISTEL(I)=LISTEL(I+1) + LISTEL(I+1)=ITEMP + ITEMP=LISTAL(I) + LISTAL(I)=LISTAL(I+1) + LISTAL(I+1)=ITEMP +C +C +C DECREMENT BACK THRU LIST WITH THIS ELEMENT + IF(I.GT.1)I=I-1 +C + GO TO 20 + END + SUBROUTINE CBASE(BASE,BKEY,IOK,ICBASE, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR RODGER STADEN + CHARACTER BASE(4),BKEY(4),HELPF*(*),NEW(4) + CHARACTER PROMPT*14 + SAVE PROMPT + DATA PROMPT/'Key for base '/ + IOK = 1 + CALL YESNO(ICBASE,'Use special keys for ACTG', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ICBASE.EQ.1) THEN + IOK = 0 + RETURN + ELSE IF(ICBASE.NE.0) THEN + RETURN + END IF + I = 1 +1 CONTINUE + IF(I.LT.5) THEN + PROMPT(14:14) = BASE(I) + L = 1 + CALL GETSTR(PROMPT,BKEY(I),NEW,4,L,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(INFLAG.EQ.2) RETURN + IF(NEW(1).EQ.';') THEN + WRITE(KBOUT,*)' ; is not allowed' + GO TO 1 + END IF + IF(L.NE.0) BKEY(I) = NEW(1) + I = I + 1 + GO TO 1 + END IF + CALL CCASEA(BKEY,4,2) + IOK = 0 + END + SUBROUTINE CCTA(SEQ,ID) + CHARACTER SEQ(ID),COM,AS + SAVE COM,AS + DATA COM/','/,AS/'*'/ + DO 10 I = 1,ID + IF(SEQ(I).EQ.COM) SEQ(I) = AS +10 CONTINUE + END + SUBROUTINE CEDT(KBIN,KBOUT, + +GELNOS,GELSTR,GELEND, + +MAXDB,GELNO,LINNO,MAXLIN,RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,NGELS,NCONTS,GEL,GEL2,MAXGEL,LINCON,PERCD,IDM, + +IHELPS,IHELPE,HELPF,IDEVH,IDEV,IDEV1,IDEV2,IDEV3,LINLEN, + +FILNAM,LLINO,IOK) + INTEGER RELPG(MAXDB),LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER LINEIN*80,GEL(MAXGEL),GEL2(MAXGEL) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),HELPF*(*) + INTEGER GELNOS(MAXDB),GELSTR(MAXDB),GELEND(MAXDB) + INTEGER GELNO(MAXLIN,2),LINNO(MAXLIN,2),RREG + WRITE(KBOUT,*)'Identify contig to edit' + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + +LLINO,IOK,IDBSIZ,KBIN,KBOUT,IDEV3, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,1,IOK,KBIN,KBOUT, + +'Name for temporary editing file', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + LREG = 1 + RREG = RELPG(LINCON) + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + +GEL,LLINO,LINCON,LREG,RREG,GEL2,I1,I2,0,I,IDBSIZ,IDEV,KBOUT, + +IDEV2,IDEV3,LINLEN,PERCD,MAXGEL,IDM) + CLOSE(UNIT=IDEV) + CALL CEDIT(FILNAM) + CALL YESNO(IWANT,'Put edited contig into database', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IWANT.NE.0) RETURN + CALL OPENRS(IDEV,FILNAM,IOK,LRECL,2) + IF(IOK.NE.0) RETURN + KGEL = LLINO + CALL BUSY(KBOUT) + CALL POSTC(GELNOS,GELSTR,GELEND,LINEIN, + +IOK,KBOUT,IDEV,MAXDB,GELNO,LINNO,MAXLIN,RELPG,LNGTHG, + +LNBR,RNBR,IDBSIZ,KGEL) + IF(IOK.NE.0) THEN + CLOSE(UNIT=IDEV) + RETURN + END IF + REWIND IDEV + CALL NEWDB(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,KBOUT,IDEV, + +IDEV1,IDEV2,IDEV3,MAXDB,GEL,MAXGEL,LINCON,KGEL,IOK) + CLOSE(UNIT=IDEV) + END +C +C CFGEL +C +C ROUTINE TO COMPARE A STRING OF WORD NUMBERS FOR A GEL WITH A SERIES +C OF ARRAYS REPRESENTING A CONSENSUS SEQUENCE. WE LOOK FOR OCCURENCES +C OF PAIRS OF WORDS (EACH WORD IS LENGTH CHARS LONG AND SO TOTAL MATCH IS +C 2*LENGTH CHARS LONG). THE ARRAYS SENT ARE OF SIZE 4**LENGTH (LE4) + SUBROUTINE CFGEL(GELN,IDIMG,POSNS,IDIM,WORDP,WORDN,LENGTH,LE4, + +SAVPG, + +SAVPS,SAVL,IDSAV,SEQ,GEL,MINMAT,IFAIL,KBOUT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),GEL(IDIMG) + INTEGER POSNS(IDIM),WORDP(LE4),SAVPS(IDSAV),SAVPG(IDSAV) + INTEGER GELN(IDIMG),SAVL(IDSAV) + INTEGER WORDN(LE4) + INTEGER W1,W2,PS1W1,PS1W2 + INTEGER CTONUM + EXTERNAL CTONUM +C bug fix 27-2-92: if sequence had different case matches would +C not be extended correctly because we look for identical characters +C switched to using ctonum + IDSAVM=IDSAV + IDSAV=0 +C LENGTH OF PAIR OF WORDS + LX2=2*LENGTH +C NUMBER OF PAIRS OF WORDS OF LENGTH LENGTH IN GEL + NW=IDIMG-(LX2-1) +C LOOP FOR EACH START POINT + DO 200 I=1,NW +C IS THIS WORD A ZERO? + W1=GELN(I) + IF(W1.EQ.0)GO TO 200 +C POINT TO NEXT WORD OF PAIR + W2=GELN(I+LENGTH) + IF(W2.EQ.0)GO TO 200 +C DOES W1 EXIST IN SEQ? + N1S1=WORDN(W1) + IF(N1S1.EQ.0)GO TO 200 + N2S1=WORDN(W2) + IF(N2S1.EQ.0)GO TO 200 +C BOTH EXIST, SO POINT TO THE FIRST + LENGTH + PS1W1=WORDP(W1)+LENGTH +C LOOP FOR ALL PAIRS + DO 50 J=1,N1S1 +C POINT TO FIRST W2 BECAUSE IT IS IN WORDP NOT POSNS + PS1W2=WORDP(W2) +C LOOP FOR THESE + DO 40 K=1,N2S1 +C ARE THIS PAIR LENGTH APART? + N=PS1W1-PS1W2 + IF(N.NE.0)GO TO 20 +C THEY ARE SO, IF REQUIRED LOOK FOR REST OF MATCH + LMAT=LX2 + IF(MINMAT.EQ.LX2)GO TO 15 + IPC=PS1W2+LENGTH + IPG=I+LX2 +16 CONTINUE + IF(IPG.GT.IDIMG)GO TO 15 + IF(IPC.GT.IDIM)GO TO 15 +C + IF(CTONUM(SEQ(IPC)).NE.CTONUM(GEL(IPG)))GO TO 15 + LMAT=LMAT+1 + IPC=IPC+1 + IPG=IPG+1 + GO TO 16 +15 CONTINUE +C IS MATCH LONG ENOUGH? + IF(LMAT.LT.MINMAT)GO TO 20 + IDSAV=IDSAV+1 + IF(IDSAV.LE.IDSAVM)GO TO 18 + WRITE(KBOUT,1000)IDSAVM +1000 FORMAT(' More than ',I6,' matches. Search aborted') + IFAIL=1 + RETURN +18 CONTINUE + SAVL(IDSAV)=LMAT + SAVPG(IDSAV)=I + SAVPS(IDSAV)=PS1W1-LENGTH +20 CONTINUE +C POINT TO NEXT W2 + PS1W2=POSNS(PS1W2) +40 CONTINUE +C ALL TRIED THIS PS1W1, TRY NEXT + PS1W1=POSNS(PS1W1-LENGTH)+LENGTH +50 CONTINUE +200 CONTINUE + IFAIL=0 + RETURN + END + INTEGER FUNCTION CHAINL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,IIN) +C AUTHOR: RODGER STADEN +C RETURNS CONTIG LEFT GEL NUMBER OR ZERO FOR ERROR + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + I = IIN + J = I + CHAINL = 0 +10 CONTINUE + IF(I.NE.0)THEN + J = I + I = LNBR(I) + IF(I.EQ.IIN)RETURN + GO TO 10 + END IF + CHAINL = J + END +C +C CHANGE +C +C ROUTINE TO EXCHANGE ALL THE CHARS IN A CHARACTER ARRAY USING +C A PAIR OF LOOKUP ARRAYS SENT BY CALLING PROG +C +C + SUBROUTINE CHANGE(SEQ,IDIM1,CHAR1,CHAR2,IDIM2,ELSE) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1) + CHARACTER CHAR1(IDIM2),CHAR2(IDIM2) + CHARACTER ELSE + DO 100 I=1,IDIM1 +C + DO 50 J=1,IDIM2 +C + IF(SEQ(I).NE.CHAR1(J))GO TO 50 +C MATCH SO EXCHANGE CHARS + SEQ(I)=CHAR2(J) + GO TO 100 +50 CONTINUE + SEQ(I)=ELSE +100 CONTINUE +C + RETURN + END + CHARACTER*1 FUNCTION CHARSL(I) + CHARACTER C*6 + SAVE C + DATA C/'ctag*-'/ + CHARSL = C(I:I) + END + CHARACTER*1 FUNCTION CHARSU(I) + CHARACTER C*6 + SAVE C + DATA C/'CTAG*-'/ + CHARSU = C(I:I) + END + INTEGER FUNCTION CHNRP(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LGEL,NCONT, + +LREG) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + I = LGEL + CHNRP = 0 +10 CONTINUE + IF(I.NE.0) THEN + IF(RELPG(I).LE.LREG) THEN + I = RNBR(I) + GO TO 10 + END IF + CHNRP = I + RETURN + END IF + END + INTEGER FUNCTION CHNRP1(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ, + +LGEL,LREG) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + I = LGEL + CHNRP1 = 0 +10 CONTINUE + IF(I.NE.0) THEN + IF(RELPG(I)+ABS(LNGTHG(I))-1.LT.LREG) THEN + I = RNBR(I) + GO TO 10 + END IF + CHNRP1 = I + RETURN + END IF + END + INTEGER FUNCTION CLEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,IIN) +C AUTHOR: RODGER STADEN +C RETURNS CONTIG LEFT GEL NUMBER OR ZERO FOR ERROR + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + I = IIN + CLEN= 0 + LEN = 0 +10 CONTINUE + IF(I.NE.0)THEN + LEN = MAX(LEN,(RELPG(I) + ABS(LNGTHG(I)) - 1)) + I = RNBR(I) + IF(I.EQ.IIN)RETURN + GO TO 10 + END IF + CLEN = LEN + END + SUBROUTINE CLIST(GELNO1,LINNO1,IGEL1,GELNO2,LINNO2, + +IGEL2,GELNOS,GELSTR,GELEND,IUNIQ1,IUNIQ,KBOUT,IOK) +C AUTHOR: RODGER STADEN + INTEGER GELNO1(IGEL1),GELNO2(IGEL2),GELNOS(IUNIQ1) + INTEGER GELSTR(IUNIQ1),GELEND(IUNIQ1) + INTEGER LINNO1(IGEL1),LINNO2(IGEL2) + EXTERNAL INLIST +C GELNOS === GEL NUMBERS (GELNOS) +C GELSTR === GEL START LINES +C GELEND === GEL END LINES +C GELNO === GEL NUMBERS PER STRIP +C LINNO === GEL LINE NUMBERS PER STRIP +C IGEL === NUMBER OF GELS PER STRIP +C LINENO === CURRENT LINE NUMBER +C +C WHICH GELS IN GELNO2 DO NOT APPEAR IN GELNO1 +C IE HAVE STARTED IN GELNO2 + DO 20 I=1,IGEL2 + MATCH=INLIST(GELNO1,IGEL1,GELNO2(I)) + IF(MATCH.EQ.0)THEN +C NO MATCH SO NEW +C PUT IN GELSTR + IUNIQ=IUNIQ+1 + GELNOS(IUNIQ)=GELNO2(I) + GELSTR(IUNIQ)=LINNO2(I) + END IF +20 CONTINUE +C WHICH GELS IN GELNO1 DO NOT APPEAR IN GELNO2 +C IE WHICH HAVE ENDED IN GELNO1 + DO 10 I=1,IGEL1 + MATCH=INLIST(GELNO2,IGEL2,GELNO1(I)) + IF(MATCH.EQ.0)THEN +C NO MATCH SO MUST HAVE ENDED +C WHERE IS IT STORED IN GELNOS? + MATCH=INLIST(GELNOS,IUNIQ,GELNO1(I)) + IF(MATCH.NE.0)THEN + GELEND(MATCH)=LINNO1(I) + GO TO 10 + END IF +C ERROR + WRITE(KBOUT,1000)GELNO1(I) +1000 FORMAT( ' Error: gel number ',I5, + + ' expected but not found in list') + IOK = 1 + RETURN + END IF +10 CONTINUE + IOK = 0 + RETURN + END +C +C CMPLMT +C +C SUBROUTINE TO REVERSE AND COMPLEMENT GELS AND DATA BASE +C THE POSITIONS OF THE RIGHT ENDS OF GELS ARE FIRST STORED +C IN RELPG THEN WE DO A BUBBLE SORT ON THESE POSITIONS +C UPDATING RELATIONSHIPS AS WE GO +C ALSO SEQUENCES ARE COMPLEMENTED, SIGNS OF LENGTH ARE +C MULTIPLIED BY -1 AND THE CONTIG LINE IS ALTERED + SUBROUTINE CMPLMT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LINCON,LLINO,GEL,IDBSIZ,KBOUT,IDEVR,IDEVW,MAXGEL) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + INTEGER X +C + WRITE(KBOUT,1000)LLINO +1000 FORMAT( ' Complementing contig',I6) +C CHAIN THRU AND PUT RIGHT ENDS IN RELPG + N=LLINO +10 CONTINUE + RELPG(N)=RELPG(N)+(ABS(LNGTHG(N)))-1 + IF(RNBR(N).EQ.0)GO TO 20 + N=RNBR(N) + GO TO 10 +20 CONTINUE +C +C NOW EFFECTIVELY BUBBLE SORT ON RELPG + N=RNBR(LINCON) + GO TO 22 +21 CONTINUE + N=NL + IF(I1.GT.0)N=I2 +22 CONTINUE + NL=LNBR(N) + IF(NL.EQ.0)GO TO 30 + I1=0 +23 CONTINUE + IF(RELPG(N).GE.RELPG(NL))GO TO 21 +C NOT IN CORRECT ORDER SO CHAIN ALONG UNTIL CORRECT,THEN COME +C BACK TO THIS POINT AND CONTINUE +C IF FIRST MOVE THIS LINE SET POINTER TO CURRENT POSITION + IF(I1.EQ.0)I2=N + I1=1 +C +C EXCHANGE NEIGHBOURS. CURRENTLY LOOKING AT N AND ITS LEFT +C NBR, AND THE LEFT NBR IS FURTHER RIGHT THAN N +C FIX UP POINTERS TO LEFT AND RIGHT OF THESE TWO + M=LNBR(NL) + IF(M.NE.0)RNBR(M)=N + M=RNBR(N) + IF(M.NE.0)LNBR(M)=NL + LNBR(N)=LNBR(NL) + LNBR(NL)=N + RNBR(NL)=RNBR(N) + RNBR(N)=NL +C CHAIN BACK THRU LIST WITH THIS LINE + N=RNBR(NL) + IF(N.EQ.0)GO TO 21 +C IE END MET + GO TO 23 +30 CONTINUE +C FINISH WITH LEFT END IN N +40 CONTINUE +C NOW REVERSE NBRS SO CHAIN BACK RIGHT + NL=RNBR(N) + IF(NL.EQ.0)GO TO 50 + RNBR(N)=LNBR(N) + LNBR(N)=NL + N=NL + GO TO 40 +50 CONTINUE +C NEED TO FIX UP NEW LEFT END + RNBR(N)=LNBR(N) + LNBR(N)=0 +C ALL POINTERS FIXED NOW DO RELATIVE POSITION +C FINISH WITH LEFT END IN N +C SO CHAIN BACK RIGHT +C SAVE RIGHT LINE NUMBER + NL=N + X=RELPG(N) +60 CONTINUE + RELPG(N)=1+(-1*(RELPG(N)-X)) + IF(RNBR(N).EQ.0)GO TO 70 + N=RNBR(N) + GO TO 60 +70 CONTINUE +C NOW FIX CONTIG LINE + LNBR(LINCON)=NL + RNBR(LINCON)=N +C WRITE NEW CONTIG LINE + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) +C WRITE(IDEVR,REC=LINCON)RELPG(LINCON),LNGTHG(LINCON),LNBR(LINCON), +C 1RNBR(LINCON) +C NOW REVERSE AND COMPLEMENT GELS + N=NL +80 CONTINUE +C READ(IDEVW,REC=N)GEL + CALL READW(IDEVW,N,GEL,MAXGEL) + M=ABS(LNGTHG(N)) + CALL SQREV(GEL,M) + CALL SQCOM(GEL,M) + CALL WRITEW(IDEVW,N,GEL,MAXGEL) +C WRITE(IDEVW,REC=N)GEL +C CHANGE SIGNS + LNGTHG(N)=-1*LNGTHG(N) +C WRITE NEW GEL LINE + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) +C WRITE(IDEVR,REC=N)RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) +C ANY MORE? + N=RNBR(N) + IF(N.NE.0)GO TO 80 +C NO MORE + RETURN + END +C CONSEN +C CALCULATES A CONSENSUS USING THE RULES OUTLINED IN THE DOCUMENTATION +C AND SUBROUTINE SUMMER +C UNIT IDEV IS USED FOR OUTPUT + SUBROUTINE CONSEN(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,IDBSIZ,TEMP,CHRSIZ,MAXGL2, + +KBIN,KBOUT,IDEVW,IDEV,NAMCON, + +IHELPS,IHELPE,FILEH,IDEVH,MAXGEL,IDM,PERCD,IDEVN,LLINO) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),ANS,CHRSIZ + INTEGER LREG,RREG,X,Y,TEMP(CHRSIZ,MAXGL2) + CHARACTER SEQ1(IDIM1) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + CHARACTER NAMPRO*(*) + CHARACTER NAMCON*(*) +100 CONTINUE + ISTART=1 + NAMCON = ' ' + CALL OPENF1(IDEV,NAMCON,1,IOK,KBIN,KBOUT, + +'Name for consensus file', + +IHELPS,IHELPE,FILEH,IDEVH) + IF(IOK.NE.0)RETURN + CALL YESNO(ANS,'Make consensus for whole database', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) RETURN + IF(ANS.EQ.1)GO TO 150 + N=IDBSIZ-NCONTS + CALL BUSY(KBOUT) + DO 110 I=N,IDBSIZ-1 + J=LNBR(I) + X=1 + Y=RELPG(I) + IF((ISTART+19+Y).GT.IDIM1)THEN + WRITE(KBOUT,1009)IDIM1 +1009 FORMAT( + + ' Maximum consensus length(',I6,') exceeded,',/, + + ' calculation aborted') + RETURN + END IF + CALL ADDTIT(SEQ1(ISTART),NAMPRO,J,ISTART) + CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + SEQ1(ISTART),Y,GEL,X,Y,J,IDBSIZ,TEMP,CHRSIZ,MAXGL2, + + IDEVW,MAXGEL,IDM,PERCD) + ISTART=ISTART+Y +110 CONTINUE + ISTART=ISTART-1 + CALL FMTDK(IDEV,SEQ1,ISTART) + GO TO 400 +150 CONTINUE + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LINCON,LLINO,IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) + IF(IERR.NE.0)GO TO 400 + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IERR) + IF(IERR.NE.0)GO TO 400 + IDIM2=RREG-LREG+1 + IF((ISTART+19+IDIM2).GT.IDIM1)THEN + WRITE(KBOUT,1009)IDIM1 + RETURN + END IF + CALL BUSY(KBOUT) + CALL ADDTIT(SEQ1(ISTART),NAMPRO,LLINO,ISTART) + CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ1(ISTART),IDIM2,GEL,LREG,RREG,LLINO,IDBSIZ,TEMP, + +CHRSIZ,MAXGL2,IDEVW,MAXGEL,IDM,PERCD) + ISTART=ISTART+IDIM2 +300 CONTINUE + CALL YESNO(ANS,'Select another contig', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.EQ.0) GO TO 150 + ISTART=ISTART-1 + CALL FMTDK(IDEV,SEQ1,ISTART) +400 CONTINUE + CALL YESNO(ANS,'Make another consensus', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.EQ.0)GO TO 100 + RETURN + END + SUBROUTINE COPYM(JLEFTS,ILEFTS,JLC,ILC, + +JPOSC,IPOSC,JSENSE,ISENSE,JLLINO,LLINO, + +JJOINT,JOINT,JTOTPC,ITOTPC,JTOTPG,ITOTPG, + +JTYPE,ITYPE,JDOUT,IDOUT,JDIM22,IDIM22, + +SEQG3,SEQG2,SEQC3,SEQC2,PERMS,PERMIS) + CHARACTER SEQG3(JDIM22),SEQG2(JDIM22),SEQC3(JDOUT),SEQC2(JDOUT) + ILEFTS = JLEFTS + ILC = JLC + IPOSC = JPOSC + IPOSG = JPOSG + ISENSE = JSENSE + LLINO = JLLINO + JOINT = JJOINT + ITOTPC = JTOTPC + ITOTPG = JTOTPG + ITYPE = JTYPE + IDOUT = JDOUT + IDIM22 = JDIM22 + CALL SQCOPY(SEQG3,SEQG2,JDIM22) + CALL SQCOPY(SEQC3,SEQC2,JDOUT) + PERMIS = PERMS + END + SUBROUTINE CRUNS(GEL,IFIRST,ILAST,RUN) + CHARACTER GEL*50,RUN*50,PAD + SAVE PAD + DATA PAD/'*'/ +C MARK RUNS IN THIS CONSENSUS SO THAT +C ATGGGCC BECOMES +C ** * + RUN(1:ILAST) = ' ' + DO 10 I = IFIRST+1,ILAST +C MUST ALLOW FOR THE EXTEMELY UNLIKELY CASE OF A RUN OF DASHES +C IN THE CONSENSUS + IF(GEL(I:I).NE.'-')THEN + IF(GEL(I:I).EQ.GEL(I-1:I-1)) RUN(I:I) = PAD + END IF +10 CONTINUE + END +C SUBROUTINE DALIGN +C +C COUNTS MISMATCHES AND DISPLAYS OVERLAP. + SUBROUTINE DALIGN(SEQC2,SEQG2,SEQ3,MAXGEL,IDOUT,IDIM2, + +JOINT,ITYPE,X,KBOUT,IFAIL) +C AUTHOR: RODGER STADEN + CHARACTER SEQC2(MAXGEL),SEQG2(MAXGEL),SEQ3(MAXGEL) + CHARACTER PAD,DASH + SAVE PAD,DASH + DATA PAD,DASH/',','-'/ + IENDG=1 + IENDC=JOINT +C ONLY LOOK AT OVERLAP WHICH IS FROM JOINT FOR LEFT TYPE JOIN + IF(ITYPE.EQ.1)THEN + IENDG=JOINT + IENDC=1 + END IF +100 CONTINUE +C LENGTH OF OVERLAP? + LG=IDIM2-IENDG+1 + LO=MIN(IDOUT,LG) +C SAVE RAW DATA + CALL SQCOPY(SEQG2,SEQ3,IDIM2) + CALL MSTLKL(SEQ3,IDIM2) + X=FLOAT(LO) + Y=X + K=IENDG+LO-1 +C POINT TO CONSENSUS + J=0 +C CHECK FOR OVERFLOW + IF(K.GT.MAXGEL)THEN + WRITE(KBOUT,1001) +1001 FORMAT( + +' Matching region too long for routine dalign. Alignment aborted') + IFAIL=1 + RETURN + END IF + DO 200 I=IENDG,K + J=J+1 + IF(SEQC2(J).EQ.SEQ3(I))GO TO 200 +C IF(SEQ3(I).EQ.DASH)GO TO 200 +C IF(SEQC2(J).EQ.DASH)GO TO 200 +C IF(SEQC2(J).EQ.PAD)GO TO 200 + X=X-1. +200 CONTINUE + X=(Y-X)*100./Y + WRITE(KBOUT,1000)X +1000 FORMAT(' Percentage mismatch after alignment = ',F4.1) + WRITE(KBOUT,1002) +1002 FORMAT(' Best alignment found') + CALL SQMTCH(SEQC2(1),SEQG2(IENDG),SEQ3,LO) + CALL FMT4LN(SEQC2(1),SEQG2(IENDG),SEQ3,LO,IENDC,IENDG,KBOUT) + IFAIL=0 + END +C DBCHEK +C TO CHECK THE SELF CONSISTENCY OF A DB +C ARE ALL NEIGHBOURS HOLDING HANDS OR DO SOME HAVE +C GELS WHICH DO NOT BELONG TO ANY CONTIG? OR WORSE BELONG TO MORE +C THAN ONE CONTIG? +C ARE THERE ANY LOOPS IN CONTIGS (THE WORST PROBLEM) +C ARE ALL RELATIVE POSITIONS IN SAME ORDER AS NEIGHBOURS? +C ARE THERE ANY CONTIGS OF LENGTH <1 ? +C ARE THERE ANY GELS OF ZERO LENGTH ? +C RETURN ERROR CODE OF ZERO FOR ALL OK, 1 FOR GELS NOT USED +C AND 2 FOR ALL OTHER ERRORS + SUBROUTINE DBCHEK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +TEMP,IERR,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER TEMP(IDBSIZ) + IERR=0 +C HAND HOLDING OK? + DO 100 I=1,NGELS + K=LNBR(I) + IF(K.EQ.0)GO TO 50 + IF(RNBR(K).EQ.I)GO TO 50 + WRITE(KBOUT,1000)I +1000 FORMAT(' Hand holding problem for gel reading',I6) + WRITE(KBOUT,1001)I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) + WRITE(KBOUT,1001)K,RELPG(K),LNGTHG(K),LNBR(K),RNBR(K) +1001 FORMAT(' ',5I6) + IERR=2 +50 CONTINUE + K=RNBR(I) + IF(K.EQ.0)GO TO 100 + IF(LNBR(K).EQ.I)GO TO 100 + WRITE(KBOUT,1000)I + WRITE(KBOUT,1001)I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) + WRITE(KBOUT,1001)K,RELPG(K),LNGTHG(K),LNBR(K),RNBR(K) + IERR=2 +100 CONTINUE +C +C ALL GELS IN CONTIGS OR IN MORE THAN ONE CONTIG? +C TEMP IS USED TO COUNT THE NUMBER OF TIMES EACH GEL IS USED + CALL FILLI(TEMP,IDBSIZ,0) + N=IDBSIZ-NCONTS +C COUNT NUMBER OF PASSES THRU LOOP 320 TO 300. IF THIS EXCEEDS +C THE DATABASE SIZE A LOOP HAS BEEN FOUND! + ICOUNT=0 + DO 300 I=N,IDBSIZ-1 + II=I + K=LNBR(I) + J=RNBR(I) + IF((K.NE.0).AND.(J.NE.0))GO TO 310 +C THIS CONTIG POINTS TO ZERO GEL NUMBER AS LEFT OR RIGHT END! + WRITE(KBOUT,1002)I +1002 FORMAT(' Contig',I4,' has zero gel numbers') + IERR=2 + GO TO 290 +310 CONTINUE + IF((LNBR(K).EQ.0).AND.(RNBR(J).EQ.0))GO TO 290 +C THIS LEFT END IS NOT A LEFT END OR THIS RIGHT END IS NOT A RIGHT END + WRITE(KBOUT,1004)I +1004 FORMAT(' The end gels of contig',I4,' have outward neighbours') + IERR=2 +290 CONTINUE +C DOES THE CONTIG HAVE SOME +VE LENGTH? + IF(RELPG(I).GT.0)GO TO 320 + WRITE(KBOUT,1010)I +1010 FORMAT(' The contig on line number',I4,' has zero length') + IERR=2 +320 CONTINUE + TEMP(K)=TEMP(K)+1 + ICOUNT=ICOUNT+1 + IF(ICOUNT.GT.IDBSIZ)GO TO 601 + K=RNBR(K) + IF(K.NE.0)GO TO 320 +300 CONTINUE + DO 400 I=1,NGELS + IF(TEMP(I).EQ.1)GO TO 390 + IF(TEMP(I).EQ.0)GO TO 410 + WRITE(KBOUT,1005)I,TEMP(I) +1005 FORMAT(' Gel number ',I6,' is used ',I6,' times') + IERR=2 + GO TO 400 +390 CONTINUE +C DOES THE GEL HAVE NONZERO LENGTH? (ONLY CHECK THOSE USED ONCE) + IF(LNGTHG(I).NE.0)GO TO 400 + WRITE(KBOUT,1011)I +1011 FORMAT(' Gel number',I6,' has zero length') + IERR=2 + GO TO 400 +410 CONTINUE + WRITE(KBOUT,1006)I +1006 FORMAT(' Gel number ',I6,' is not used') +C NEED TO INCREMENT THE ERROR COUNT NOT RESET IT TO A LOWER VALUE! + IF(IERR.LT.2)IERR=1 +400 CONTINUE +C ALL RELATIVE POSNS OK? + N=IDBSIZ-NCONTS + DO 500 I=N,IDBSIZ-1 + K=LNBR(I) + IF(K.EQ.0)GO TO 500 +510 CONTINUE + J=RNBR(K) + IF(J.EQ.0)GO TO 500 + IF(RELPG(K).GT.RELPG(J))GO TO 520 + K=J + GO TO 510 +520 CONTINUE + WRITE(KBOUT,1007)K,RELPG(K),J,RELPG(J) +1007 FORMAT(' Gel number',I6,' with position',I6, + + ' is the left neighbour of', + + /,' gel number',I6,' with position',I6) + K=J + IERR=2 + GO TO 510 +500 CONTINUE + IF(IERR.EQ.0)WRITE(KBOUT,1013) +1013 FORMAT(' Database is logically consistent') + RETURN +601 CONTINUE + IERR=2 + WRITE(KBOUT,1008)II +1008 FORMAT(' Loop in contig',I6,/, + +' No further checking done but gel numbers follow') + CALL FILLI(TEMP,IDBSIZ,0) + K=LNBR(II) +710 CONTINUE + TEMP(K)=TEMP(K)+1 + WRITE(KBOUT,1009)K +1009 FORMAT(' ',I6) + IF(TEMP(K).GT.1)RETURN + K=RNBR(K) + GO TO 710 + END + SUBROUTINE DBEDIT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,LINCON,IMAXL,IMAXR,IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW, + +IHELPS,IHELPE,FILEH,IDEVH,MAXGEL) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN +C SUBROUTINE TO EDIT GEL READINGS IN DATA BASE + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER POSN,Y,X + CHARACTER GEL(MAXGEL) + CHARACTER CHARS(80),PROMPT*32 + PARAMETER (MAXPRM = 32) + CHARACTER PRMPT(3)*(MAXPRM) + SAVE PROMPT + DATA PROMPT/'Characters for gel reading '/ +1 CONTINUE +C CALL BELL(1,KBOUT) +C CALL DBMENU(5,NOPT,IHELPS,IHELPE,FILEH,IDEVH, +C +KBIN,KBOUT) + PRMPT(1) = 'Insert' + PRMPT(2) = 'Delete' + PRMPT(3) = 'Change' + NOPT = 1 + CALL RADION('Edit options', PRMPT, 3, NOPT, IHELPS, + + IHELPE, FILEH, IDEVH, KBIN, KBOUT) + IF(NOPT.EQ.-1)RETURN + IF(NOPT.EQ.3)GO TO 200 +10 CONTINUE + MN = 1 + MX = RELPG(LINCON) + POSN = 0 + IF(IMAXL.GT.0) THEN + MN = IMAXL + MX = IMAXR + END IF + CALL GETINT(MN,MX,POSN, + +'Position to edit', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + IF(IVAL.EQ.0) GO TO 1 + POSN = IVAL +20 CONTINUE + MN = 1 + MX = 50 + NC = 1 + CALL GETINT(MN,MX,NC, + +'Number of characters', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + NC = IVAL +C NEED TO CHECK FOR DELETION TO END OF CONTIG + Y=POSN+NC + K=RELPG(LINCON)-POSN+1 + IF((NOPT.EQ.2).AND.(Y.GT.RELPG(LINCON)))NC=K +C NOW FIND FIRST CHAR THAT OVERLAPS REGION + LLINO=LNBR(LINCON) +30 CONTINUE + X=RELPG(LLINO)+ABS(LNGTHG(LLINO))-1 + IF(X.GE.POSN)GO TO 40 + LLINO=RNBR(LLINO) + GO TO 30 +40 CONTINUE + CALL READW(IDEVW,LLINO,GEL,MAXGEL) +C CALC POSN IN THIS GEL TO EDIT + K=POSN-RELPG(LLINO)+1 + IF(NOPT.EQ.2)GO TO 100 +50 CONTINUE + WRITE(PROMPT(28:),1005)LLINO +1005 FORMAT(I5) + LC = NC + CALL FILLC(CHARS,NC,'*') + CALL GETSTR(PROMPT,CHARS,CHARS,NC,LC,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) GO TO 1 + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 50 + END IF +C MOVE THE DATA RIGHT + M=ABS(LNGTHG(LLINO)) + LNGTHG(LLINO)=LNGTHG(LLINO)+SIGN(NC,LNGTHG(LLINO)) + N=ABS(LNGTHG(LLINO)) + IF(N.GT.MAXGEL)THEN + WRITE(KBOUT,2000)LLINO +2000 FORMAT( + +' Data pushed off end of gel',I5,' During insertion') + NM512=N-MAXGEL + N=MAXGEL +C SET M SO THAT THE DATA AT THE END IS LOST + M=M-NM512 + LNGTHG(LLINO)=SIGN(N,LNGTHG(LLINO)) + END IF + J=M-K+1 + DO 55 I=1,J + GEL(N)=GEL(M) + N=N-1 + M=M-1 +55 CONTINUE +C PERFORM THE INSERTION + IC=0 + DO 60 I=K,MIN(MAXGEL,K+NC-1) + IC=IC+1 +C DONT ALLOW SPACES - SET THEM TO *'S + IF(CHARS(IC).EQ.' ')CHARS(IC)='*' + GEL(I)=CHARS(IC) +60 CONTINUE + CALL WRITEW(IDEVW,LLINO,GEL,MAXGEL) + CALL WRITER(IDEVR,LLINO,RELPG(LLINO),LNGTHG(LLINO), + +LNBR(LLINO),RNBR(LLINO)) +65 CONTINUE + LLINO=RNBR(LLINO) + IF(LLINO.EQ.0)GO TO 70 + IF(RELPG(LLINO).GT.POSN)GO TO 70 + X=RELPG(LLINO)+ABS(LNGTHG(LLINO))-1 + IF(X.LT.POSN)GO TO 65 + GO TO 40 +70 CONTINUE +C INSERTS FINISHED SO NEED TO INCREMENT ALL THOSE GELS TO RIGHT + LLINO=LNBR(LINCON) +75 CONTINUE + IF(RELPG(LLINO).GT.POSN)GO TO 80 +76 CONTINUE + LLINO=RNBR(LLINO) + IF(LLINO.EQ.0)GO TO 90 + GO TO 75 +80 CONTINUE + RELPG(LLINO)=RELPG(LLINO)+(NC) + CALL WRITER(IDEVR,LLINO,RELPG(LLINO),LNGTHG(LLINO), + +LNBR(LLINO),RNBR(LLINO)) + GO TO 76 +90 CONTINUE + RELPG(LINCON)=RELPG(LINCON)+(NC) + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) + GO TO 1 +CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC +C DELETE +100 CONTINUE +C +C MOVE DATA TO LEFT + M=K+NC +C POSN TO MOVE TO + N=MAX(1,K) +C NUMBER TO MOVE + L=ABS(LNGTHG(LLINO))-M+1 +C NONE TO MOVE? + IF(L.GT.0) THEN + DO 160 I=1,L + GEL(N)=GEL(M) + N=N+1 + M=M+1 +160 CONTINUE + END IF +C NEDE TO FIX LENGTHS AND REL POSNS OF GELS EDITED +C 3 CLASSES GELS LOSING LEFT ENDS,RIGHT ENDS,CENTRES +C FOR LEFT ENDS K<1,RIGHT ENDS L<1 +C SO SET RELPG FOR THOSE LOSING LEFT ENDS + IF(K.LT.1)RELPG(LLINO)=POSN +C FIX LENGTHS + N=N-1 + IF(L.GT.0)LNGTHG(LLINO)=SIGN(N,LNGTHG(LLINO)) +C THOSE LOSING RIGHT ENDS + M=(POSN-RELPG(LLINO)) + IF(L.LT.1)LNGTHG(LLINO)=SIGN(M,LNGTHG(LLINO)) + CALL WRITER(IDEVR,LLINO,RELPG(LLINO),LNGTHG(LLINO), + +LNBR(LLINO),RNBR(LLINO)) + CALL WRITEW(IDEVW,LLINO,GEL,MAXGEL) +165 CONTINUE + LLINO=RNBR(LLINO) + IF(LLINO.EQ.0)GO TO 170 +C DOES IT HAVE DATA IN REGION? + X=POSN+(NC) + IF(RELPG(LLINO).GE.X)GO TO 170 + X=RELPG(LLINO)+ABS(LNGTHG(LLINO))-1 + IF(X.LT.POSN)GO TO 165 +C WITHIN + GO TO 40 +170 CONTINUE +C DELETIONS DONE SO DECREMENT ALL GELS TO RIGHT + LLINO=LNBR(LINCON) + X=POSN+(NC) +175 CONTINUE + IF(RELPG(LLINO).GE.X)GO TO 180 +176 CONTINUE + LLINO=RNBR(LLINO) + IF(LLINO.EQ.0)GO TO 190 + GO TO 175 +180 CONTINUE + RELPG(LLINO)=RELPG(LLINO)-(NC) + CALL WRITER(IDEVR,LLINO,RELPG(LLINO),LNGTHG(LLINO), + +LNBR(LLINO),RNBR(LLINO)) + GO TO 176 +190 CONTINUE + RELPG(LINCON)=RELPG(LINCON)-(NC) + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) + GO TO 1 +200 CONTINUE +C +CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC +C CHANGE + MN = 1 + MX = NGELS + LLINO = 0 + CALL GETINT(MN,MX,LLINO,'Gel number', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + IF(IVAL.EQ.0) GO TO 200 + LLINO = IVAL + WRITE(KBOUT,1022)RELPG(LLINO),LNGTHG(LLINO) +1022 FORMAT( ' This gels relative position =',I6,' and length =',I6) +220 CONTINUE + MN = RELPG(LLINO) + MX = RELPG(LLINO) + ABS(LNGTHG(LLINO)) - 1 + POSN = 0 + CALL GETINT(MN,MX,POSN, + +'Position to edit', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + IF(IVAL.EQ.0) GO TO 220 + POSN = IVAL + CALL READW(IDEVW,LLINO,GEL,MAXGEL) +225 CONTINUE + LC = 50 + NC = 0 + CALL GETSTR('New chararacters',' ',CHARS,LC,NC,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) GO TO 1 + IF(NC.EQ.0) GO TO 1 + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 225 + END IF + K=POSN-RELPG(LLINO)+1 +C COPY CHARS + DO 230 I=1,NC + IF(CHARS(I).EQ.' ')CHARS(I)='*' + GEL(K)=CHARS(I) + K=K+1 + IF(K.GT.MAXGEL) GO TO 231 +230 CONTINUE +231 CONTINUE + CALL WRITEW(IDEVW,LLINO,GEL,MAXGEL) + GO TO 1 + END +C DBPRNT +C PRINTS A DATABASE. IE ITS RELATIONSHIPS + SUBROUTINE DBPRNT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +IDEV,KBIN,KBOUT,IDEVN,LLINO, + +IHELPS,IHELPE,FILEH,IDEVH) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),LREG,RREG,ANS + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER NAMARC*10 + IF(NGELS.GT.0)CALL DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS, + +NCONTS,IDBSIZ,IDEV) + WRITE(IDEV,10011)NGELS,NCONTS +10011 FORMAT(' Number of gel readings',I5,' Number of contigs',I5) +20 CONTINUE + CALL YESNO(ANS,'Select contigs', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) RETURN + IF(ANS.EQ.0) GO TO 45 + N=IDBSIZ-NCONTS +25 CONTINUE + CALL YESNO(ANS,'Show gel readings in positional order', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) RETURN + IF(ANS.EQ.0)GO TO 41 + WRITE(IDEV,1009) +1009 FORMAT(' CONTIG LINES') + WRITE(IDEV,1000) +1000 FORMAT(' CONTIG LINE LENGTH ENDS'/ + +' LEFT RIGHT') + DO 30 I=N,IDBSIZ-1 + WRITE(IDEV,1007)I,RELPG(I),LNBR(I),RNBR(I) +30 CONTINUE +1007 FORMAT( ' ',12X,I4,2X,I6,10X,I6,2X,I6) + WRITE(IDEV,1008) +1008 FORMAT(' GEL LINES') + WRITE(IDEV,1001) +1001 FORMAT(' NAME NUMBER POSITION LENGTH NEIGHBOURS'/ + +' LEFT RIGHT') + DO 40 I=1,NGELS + CALL READN(IDEVN,I,NAMARC) + WRITE(IDEV,1006)NAMARC,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) +1006 FORMAT( ' ',A,2X,I4,2X,I7,2X,I5,2X,I6,2X,I6) +40 CONTINUE + RETURN +C +41 CONTINUE +C +C SORTED DATA + DO 43 I=N,IDBSIZ-1 + WRITE(IDEV,1021) +1021 FORMAT( ) + WRITE(IDEV,1000) + WRITE(IDEV,1007)I,RELPG(I),LNBR(I),RNBR(I) + J=LNBR(I) + WRITE(IDEV,1001) +42 CONTINUE + CALL READN(IDEVN,J,NAMARC) + WRITE(IDEV,1006)NAMARC,J,RELPG(J),LNGTHG(J),LNBR(J),RNBR(J) + J=RNBR(J) + IF(J.NE.0)GO TO 42 +43 CONTINUE + RETURN +45 CONTINUE +C SELECTED CONTIGS ONLY +C +C GET GEL NUMBER AND CONTIG NUMBER + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + +LLINO,IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) + IF(IERR.NE.0)RETURN + WRITE(IDEV,1009) + WRITE(IDEV,1000) + WRITE(IDEV,1007)LINCON,RELPG(LINCON),LNBR(LINCON),RNBR(LINCON) + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IERR) + IF(IERR.NE.0)RETURN + WRITE(IDEV,1008) + N=LLINO + WRITE(IDEV,1001) +46 CONTINUE + CALL READN(IDEVN,N,NAMARC) + WRITE(IDEV,1006)NAMARC,N,RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) + IF(RNBR(N).EQ.0)GO TO 48 + N=RNBR(N) + IF(RELPG(N).GT.RREG)GO TO 48 + GO TO 46 +48 CONTINUE + GO TO 45 + END + SUBROUTINE DBSCAN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1, + +IDIM1,GEL,IDBSIZ,TEMP3,ID1,CHRSIZ,MAXGL2,KBIN,KBOUT,IDEVW, + +IDEV,LINLEN,PERCD, + +IHELPS,IHELPE,FILEH,IDEVH,MAXGEL,LINOU1,LINOU2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEVN, + + LLINO,LINCON,LREG,RREG,MXGOOD) +C 28-7-91 added extra parameter mxgood: the max length of read +C we have confidence in + CHARACTER FILEH*(*) + PARAMETER (MAXPRM = 10) + CHARACTER PROMPT(2)*(MAXPRM) +C AUTHOR: RODGER STADEN + INTEGER RREG, RELPG(IDBSIZ),CHRSIZ + INTEGER LREG,TEMP3(ID1,CHRSIZ,MAXGL2),ANS + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),LINOU1(MAXGEL),LINOU2(MAXGEL) + CHARACTER SEQ1(IDIM1) + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LINCON,LLINO,IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) + IF(IERR.NE.0) RETURN + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IERR) + IF(IERR.NE.0) RETURN + IDIM2=RREG-LREG+1 + CALL SUMMAR(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,LREG,RREG,LLINO,PERCD,IDBSIZ, + +TEMP3,ID1,CHRSIZ,MAXGL2,IDEVW, + +MAXGEL,LINOU1,LINOU2,MXGOOD) + CALL DBSCSM(SEQ1(LREG),IDIM2,KBOUT) +160 CONTINUE + ANS = 1 + PROMPT(1) = 'List codes' + PROMPT(2) = 'Plot codes' + CALL RADION('Select results display mode',PROMPT,2,ANS, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANS.LT.1) RETURN + IF(ANS.EQ.1) THEN + CALL FMTDB(SEQ1,IDIM1,LREG,RREG,LINLEN,IDEV) + RETURN + ELSE + CALL PLTQ(SEQ1(LREG),IDIM2, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + END + SUBROUTINE DBSCNP(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1, + +IDIM1,GEL,IDBSIZ,TEMP3,ID1,CHRSIZ,MAXGL2,IDEVW,LLINO, + +PERCD,MAXGEL,LINOU1,LINOU2,LREG,RREG, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,MXGOOD) +C AUTHOR: RODGER STADEN + INTEGER RREG, RELPG(IDBSIZ),CHRSIZ + INTEGER LREG,TEMP3(ID1,CHRSIZ,MAXGL2) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),LINOU1(MAXGEL),LINOU2(MAXGEL) + CHARACTER SEQ1(IDIM1) + IDIM2=RREG-LREG+1 +C 28-7-91 added extra parameter mxgood: the max length of read +C we have confidence in + CALL SUMMAR(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,LREG,RREG,LLINO,PERCD,IDBSIZ, + +TEMP3,ID1,CHRSIZ,MAXGL2,IDEVW, + +MAXGEL,LINOU1,LINOU2,MXGOOD) + CALL PLTQ(SEQ1(LREG),IDIM2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END + SUBROUTINE DBSCSM(SEQ1,IDIM1,KBOUT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM1) + CHARACTER CODES(5) + REAL X(5) + SAVE CODES + DATA CODES/'0','1','2','3','4'/ + DO 50 J=1,5 + X(J)=0. +50 CONTINUE + DO 100 I=1,IDIM1 + DO 60 J=1,5 + IF(SEQ1(I).NE.CODES(J))GO TO 60 + X(J)=X(J)+1. + GO TO 61 +60 CONTINUE +61 CONTINUE +100 CONTINUE + SUM=0. + DO 130 J=1,5 + SUM=SUM+X(J) +130 CONTINUE + DO 140 J=1,5 + IF(SUM.NE.0)X(J)=X(J)*100./SUM +140 CONTINUE + WRITE(KBOUT,1001)X(1) +1001 FORMAT(' ',F6.2,'% OK on both strands and they agree(0)') + WRITE(KBOUT,1002)X(2) +1002 FORMAT(' ',F6.2,'% OK on plus strand only(1)') + WRITE(KBOUT,1003)X(3) +1003 FORMAT(' ',F6.2,'% OK on minus strand only(2)') + WRITE(KBOUT,1004)X(4) +1004 FORMAT(' ',F6.2,'% Bad on both strands(3)') + WRITE(KBOUT,1005)X(5) +1005 FORMAT(' ',F6.2,'% OK on both strands but they disagree(4)') + RETURN + END +C DBSTAT + SUBROUTINE DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +KBOUT) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + N=IDBSIZ-NCONTS + SUM=0. + DO 20 I=N,IDBSIZ-1 + SUM=SUM+RELPG(I) +20 CONTINUE + AV=SUM/NCONTS + WRITE(KBOUT,1020)SUM,AV +1020 FORMAT( ' Total contig length ',F10.0,' Average', + +' length ',F10.1) + SUMG=0. + DO 30 I=1,NGELS + SUMG=SUMG+FLOAT(ABS(LNGTHG(I))) +30 CONTINUE + AV=SUMG/SUM + WRITE(KBOUT,1021)SUMG +1021 FORMAT( ' Total characters in gel readings ',F10.0) + WRITE(KBOUT,1022)AV +1022 FORMAT + +( ' Average gel characters per consensus character ',F10.2) +99 CONTINUE + RETURN + END +C DELCON +C +C DELETES CONTIG FROM CONSENSUS SEQUENCE + SUBROUTINE DELCON(SEQ1,ILEFT,ILC,IDIM1) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM1) +C FIRST CHAR TO REPLACE + I1=ILEFT-20 +C FIRST CHAR TO MOVE + I2=ILEFT+ILC +C IS THIS RIGHTMOST CONTIG ANYWAY? + IF(I2.GT.IDIM1)GO TO 10 +C NUMBER TO MOVE + ID=IDIM1-I2+1 +C MOVE + CALL SQCOPY(SEQ1(I2),SEQ1(I1),ID) +C RESET LENGTH + IDIM1=I1+ID-1 + RETURN +10 CONTINUE +C RIGHTMOST CONTIG SO DONT MOVE + IDIM1=I1-1 +C + RETURN + END + SUBROUTINE DISMAT(SEQ,IDIM,GEL,IDIMG,SAVPS,SAVPG,IDSAV, + +CENDS,NENDS,IDCEND,MAXCON,KBOUT,MATCH) +C AUTHOR: RODGER STADEN + INTEGER CENDS(MAXCON) + INTEGER NENDS(MAXCON) + INTEGER SAVPS(IDSAV),SAVPG(IDSAV) + CHARACTER SEQ(IDIM),GEL(IDIMG),MATCH(IDIMG) +C EDIT 07-02-83 TO CHECK FOR CASE WHEN GEL OVERLAPS ADJACENT +C CONTIGS WITHIN THE LENGTH OF THE GEL! DONE BY HAVING A +C PARAMETER THAT STORES THE POSITION OF THE LEFT END OF THE +C NEXT CONTIG (IE THE ONE AFTER THE ONE THE CURRENT GEL OVERLAPS) +C SET IT TO A VERY LARGE VALUE INITIALLY + NEXTC=99999 +C SORT THE MATCHING WORDS INTO ASCENDING ORDER ON POSITION IN SEQ + CALL BUB2AS(SAVPS,SAVPG,IDSAV) +C LOOK FOR SEPARATE MATCHES + LEND=IDIMG-SAVPG(1)+SAVPS(1) +C WRITE(KBOUT,1000)SAVPG(1),SAVPS(1) + CALL DISMAU(SEQ,IDIM,GEL,IDIMG,SAVPS(1), + +SAVPG(1),CENDS,NENDS,IDCEND,MAXCON, + +NEXTC,KBOUT,MATCH) + DO 10 I=2,IDSAV + IF((SAVPS(I).LT.LEND).AND.(SAVPS(I).LT.NEXTC))GO TO 10 +C NEW MATCH, DISPLAY IT +C WRITE(KBOUT,1000)SAVPG(I),SAVPS(I) +C1000 FORMAT(' ',2I6) + CALL DISMAU(SEQ,IDIM,GEL,IDIMG,SAVPS(I), + +SAVPG(I),CENDS,NENDS,IDCEND,MAXCON, + +NEXTC,KBOUT,MATCH) +C RESET LEND + LEND=IDIMG-SAVPG(I)+SAVPS(I) +10 CONTINUE + RETURN + END +C +C DISMAU +C ROUTINE TO DISPLAY MATCHES +C EDITED 17-12-81 TO NOT SUBTRACT 1 FROM LCL AND LGR + SUBROUTINE DISMAU(SEQ,IDIM1,GEL,IDIMG,ISAVPS,SAVPG,CENDS,NENDS, + +IDCEND,MAXCON,NEXTC,KBOUT,MATCH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),GEL(IDIMG),MATCH(IDIMG) + INTEGER SAVPS,SAVPG,CENDS(MAXCON) + INTEGER NENDS(MAXCON) +C EDITED 07-02-83 FOR NEXTC (SEE DISMAT) +C DELETE 20 FROM END OF CONSENSUS MATCH + SAVPS=ISAVPS-19 +C FIND CONTIG CONSENSUS ENDS + JJ=1 + DO 5 J=2,IDCEND + IF(SAVPS.GT.CENDS(J))GO TO 5 +C GONE PAST SO LAST IS THE ONE + JJ=J-1 + GO TO 6 +5 CONTINUE + JJ=IDCEND +6 CONTINUE +C SUBTRACT 1 FROM END + SAVPS=SAVPS-1 +C LENGTH FROM MATCH TO LEFT OF CONTIG + LCL=SAVPS-CENDS(JJ) +C RIGHT + LCR=CENDS(JJ+1)-ISAVPS-1 +C LEFT GEL + LGL=SAVPG-1 + LGR=IDIMG-SAVPG +C NEED MIN OF EACH PAIR + LL=MIN(LCL,LGL) + LR=MIN(LCR,LGR) +C LENGTH OF OVERLAP + LM=LR+LL+1 +C DISPLAY STARTS + ICL=ISAVPS-LL + IGL=SAVPG-LL + WRITE(KBOUT,1000)NENDS(JJ) +1000 FORMAT(' Match found with vector number =',I6) + CALL SQMTCH(SEQ(ICL),GEL(IGL),MATCH,LM) + L=ICL-CENDS(JJ)-19 + CALL FMT4LN(SEQ(ICL),GEL(IGL),MATCH,LM,L,IGL,KBOUT) +C SAVE POSN OF END OF NEXT CONTIG + NEXTC=CENDS(JJ+1)+20 + RETURN + END + SUBROUTINE DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + +GEL,LLINOO,LINCON,LREG,RREG,GEL2,I1,IDIM,NOPT, + +LLINOR,IDBSIZ,IDEV,KBOUT,IDEVW,IDEVN,LINLEN,PERCD, + +MAXGEL,IDM) +C AUTHOR: RODGER STADEN + INTEGER CHRSIZ + PARAMETER (CHRSIZ = 6) + PARAMETER (IDC1 = CHRSIZ*100) + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER LREG,RREG,X,XLS2,XLS1,XRS2,XRS1,RREG2 + CHARACTER MATCH(100) + INTEGER CHARS(CHRSIZ,100),CHARS1(IDC1) + CHARACTER NAMARC*10 + CHARACTER GEL(MAXGEL) + CHARACTER GEL2(MAXGEL) + INTEGER RELPOS(10),RELPO2(10) + INTEGER GELC + INTEGER RP + INTEGER LSEQNO,RSEQNO + CHARACTER LINOUT(100) + CHARACTER MUNOTP + CHARACTER GTCONC + EXTERNAL GTCONC + EQUIVALENCE (CHARS1,CHARS) + CALL FILLI(CHARS1,IDC1,0) +C SET CONTIG NUMBER + ICON=1 + LLINO=LLINOO + NLEN=LINLEN/10 + LSEQNO=LREG + X=LINLEN+LSEQNO-1 + RSEQNO=MIN(RREG,X) +C SET LEFT GEL NUMBER FOR RIGHT CONTIG + LN2=LLINOR +C FIRST GEL NO IS LLINOO +C SET RREG FOR RIGHT CONTIG + RREG2=IDIM +C SET UP LSEQNO,RSEQNO FOR FOR NOPT=3 + XLS2=I1 + XRS2=RSEQNO-LSEQNO+XLS2 +9 CONTINUE +C IF RIGHT CONTIG SKIP NUMBER PRINTING + IF(ICON.EQ.2)GO TO 8 +C NEED TO KEEP LONGEST LINE LENGTH FOR OUTPUT OF CONSENSUS + IE=0 +C SETUP AND WRITE NUMBERS + RELPOS(1)=LSEQNO+9 + DO 5 I=2,NLEN + RELPOS(I)=RELPOS(I-1)+10 +5 CONTINUE + WRITE(IDEV,1023) + +(RELPOS(K),K=1,MIN(NLEN,MAX(1,(RSEQNO-LSEQNO+1)/10))) +1023 FORMAT( ' ',19X,10(I9,1X)) +C SET CURRENT LINE NUMBER +8 CONTINUE + GELC=LLINO +10 CONTINUE +C IS LEFT END OF CURRENT GEL >RREG + IF(RELPG(GELC).GT.RSEQNO)GO TO 200 +C ALSO NEED TO KNOW IF RIGHT END ON THIS LINE (IF .LT. NO DATA +C TO DISPLAY) + X=RELPG(GELC)+ABS(LNGTHG(GELC))-1 + IF(X.LT.LSEQNO)GO TO 190 + CALL READW(IDEVW,GELC,GEL,MAXGEL) + CALL FILLC(LINOUT,LINLEN,' ') + CALL READN(IDEVN,GELC,NAMARC) +C +C NEED TO KNOW HOW MANY CHARS TO COPY OVER TO OUTPUT LINE +C AND WHERE IN LINE TO PUT THEM +C CURRENT LINE LEFT END IS LSEQNO,RIGHT END RSEQNO +C SO LEFT START CHAR IS + X=MAX(LSEQNO,RELPG(GELC)) +C POSITION IN ARRAY LINE + LP=X-LSEQNO+1 +C RIGHT END CHAR IS + X=RELPG(GELC)+ABS(LNGTHG(GELC))-1 + X=MIN(RSEQNO,X) +C POSITION IN ARRAY LINE + RP=X-LSEQNO+1 +C LOOK FOR LONGEST LINE + IF(RP.GT.IE)IE=RP +C NEED LEFT START IN GEL + K=LSEQNO-RELPG(GELC)+1 + IF(K.LT.1)K=1 + NCOP=RP-LP+1 + IF(NCOP.GT.0)CALL SQCOPY(GEL(K),LINOUT(LP),NCOP) + N=LP+NCOP-1 + II=K-1 + IF(IDM.EQ.26)THEN + DO 50 I = LP,N + II = II + 1 + CALL PCON1(GEL(II),CHARS(1,I)) +50 CONTINUE + ELSE + DO 70 I=LP,N + II=II+1 + JJ = INDEXS(GEL(II),JSCORE) + CHARS(JJ,I) = CHARS(JJ,I) + JSCORE +70 CONTINUE + END IF + I=SIGN(GELC,LNGTHG(GELC)) + WRITE(IDEV,1020)I,NAMARC,(LINOUT(K),K=1,RP) +1020 FORMAT( ' ',I4,2X,A,2X,100A1) +C +190 CONTINUE +C NOW GET NEXT GEL TO RIGHT + GELC=RNBR(GELC) + IF(GELC.NE.0)GO TO 10 +200 CONTINUE +C CALC CONSENSUS AND WRITE IT + IF(IDM.EQ.26)THEN + DO 49 I = 1,LINLEN + LINOUT(I) = MUNOTP(CHARS(1,I)) + CHARS(1,I) = 0 +49 CONTINUE + ELSE + DO 230 I=1,LINLEN + LINOUT(I) = GTCONC(CHARS(1,I),CHRSIZ,PERCD) + CALL FILLI(CHARS(1,I),CHRSIZ,0) +230 CONTINUE + END IF + WRITE(IDEV,1019)(LINOUT(K),K=1,IE) +C IF REQUIRED WRITE COMPARISON GEL +C WHICH OPTION IN OPERATION? + IF(NOPT.EQ.2)GO TO 52 + IF(NOPT.NE.3)GO TO 250 +53 CONTINUE +C ALREADY DONE THIS LINE CONTIG2? + IF(ICON.EQ.2)GO TO 54 + ICON=2 +C NEED TO SAVE CONSENSUS FROM LEFT CONTIG + CALL SQCOPY(LINOUT,GEL2,IE) +C SAVE VALUES FROM LEFT CONTIG + XLS1=LSEQNO + XRS1=RSEQNO +C SAVE CURRENT LEFT GEL NUMBER + LN1=LLINO +C SET UP VALUES FOR RIGHT CONTIG + LSEQNO=XLS2 + RSEQNO=XRS2 +C SET LEFT GEL NUMBER + LLINO=LN2 +C GET NEXT GEL + GO TO 150 +54 CONTINUE +C SAVE CURRENT LEFT GEL NUMBER + LN2=LLINO +C SET VALUES FOR RIGHT CONTIG NEXT PASS + XLS2=XRS2+1 + XRS2=XLS2+LINLEN-1 + IF(XRS2.GT.RREG2)XRS2=RREG2 +C SET UP VALUES FOR LEFT CONTIG + LLINO=LN1 + ICON=1 + LSEQNO=XLS1 + RSEQNO=XRS1 +C SET DECREMENT FOR POINTER TO GEL2 + MMM=I1-1 +52 CONTINUE +1017 FORMAT(' NEWGEL ',100A1) +1018 FORMAT(' MISMATCH ',100A1) +1019 FORMAT(' CONSENSUS ',100A1) +1022 FORMAT( ' ',18X,100A1) + I2=I1+LINLEN-1 + IF(I2.GT.IDIM)I2=IDIM + IF(NOPT.EQ.2)WRITE(IDEV,1017)(GEL2(K),K=I1,I2) +C SET DECREMENT + IF(NOPT.EQ.2)MMM=0 +55 CONTINUE + CALL FILLC(MATCH,LINLEN,'*') + K=0 + DO 667 J=I1,I2 + K=K+1 + IF(GEL2(J-MMM).EQ.LINOUT(K))MATCH(K) = ' ' +667 CONTINUE + WRITE(IDEV,1018)(MATCH(K),K=1,IE) + RELPO2(1)=(I1)+9 + DO 240 I=2,NLEN + RELPO2(I)=RELPO2(I-1)+10 +240 CONTINUE + WRITE(IDEV,1023)(RELPO2(K),K=1,NLEN) + I1=I2+1 + I2=I2+LINLEN + IF(I2.GT.IDIM)I2=IDIM + IF(I1.GT.I2)RETURN +250 CONTINUE +C + WRITE(IDEV,1021) +1021 FORMAT( ) +C NEXT LINE LENGTH +C NEXT LENGTH IS OLD RIGHT +1 + LSEQNO=RSEQNO+1 +C NEW RIGHT IS LEFT +LENGTH + RSEQNO=LSEQNO+(LINLEN)-1 +C ARE WE OVER END OF REGION + IF(RSEQNO.GT.RREG)RSEQNO=RREG +C HAVE WE FINISHED REGION COMPLETELY + IF(RSEQNO.LT.LSEQNO) RETURN +C NOT FINISHED SO NEED TO FIND CURRENT LEFT GEL NO +C CURRENT LEFT GEL IS LLINO +C +150 CONTINUE +C NEED TO KNOW IF CURRENT LEFT GELS RIGHT END IS INSIDE REGION + X=RELPG(LLINO)+ABS(LNGTHG(LLINO))-1 + IF(X.GE.LSEQNO)GO TO 9 +C LOOK AT NEXT GEL TO RIGHT + LLINO=RNBR(LLINO) +C MAY HAVE GONE OVER END OF CONTIG????? + IF(LLINO.GT.0)GO TO 150 +300 CONTINUE + RETURN + END + SUBROUTINE EC(GEL,IDG,CON,IDC,K) + CHARACTER GEL(IDG),CON(IDC),CHARSL + EXTERNAL CHARSL,INDEXS + PARAMETER (IDASH = 6) + K = 0 + DO 10 I = 1,MIN(IDC,IDG) + JC = INDEXS(CON(I),J) + IF(JC.NE.IDASH) THEN + JG = INDEXS(GEL(I),J) + IF(JG.NE.JC) THEN + GEL(I) = CHARSL(JC) + K = K + 1 + END IF + END IF +10 CONTINUE +C WRITE(*,*)'NUMBER OF CHARS CORRECTED=',K + END + SUBROUTINE ED(GEL,IDG,CON,IDC,K) + CHARACTER GEL(IDG),CON(IDC),CHARSL + EXTERNAL CHARSL,INDEXS + K = 0 + DO 10 I = MIN(IDC,IDG),1,-1 + JC = INDEXS(CON(I),J) + IF(JC.EQ.5) THEN + IF(I.LT.IDG) CALL SQCOPY(GEL(I+1),GEL(I),IDG-I) + K = K + 1 + END IF +10 CONTINUE +C WRITE(*,*)'NUMBER OF CHARS DELETED=',K + END + SUBROUTINE EDITGL(GEL,CHARS,IDIMG,KBIN,KBOUT,MAXGEL, + +IHELPS,IHELPE,FILEH,IDEVH,ISTART) +C AUTHOR: RODGER STADEN + CHARACTER FILEH*(*) + INTEGER POSN,Y + CHARACTER GEL(MAXGEL),CHARS(MAXGEL) + PARAMETER (MAXPRM = 32) + CHARACTER PRMPT(3)*(MAXPRM) +1 CONTINUE +C CALL BELL(1,KBOUT) +C CALL DBMENU(5,NOPT,IHELPS,IHELPE,FILEH,IDEVH, +C +KBIN,KBOUT) + PRMPT(1) = 'Insert' + PRMPT(2) = 'Delete' + PRMPT(3) = 'Change' + NOPT = 1 + CALL RADION('Edit options', PRMPT, 3, NOPT, IHELPS, + + IHELPE, FILEH, IDEVH, KBIN, KBOUT) + IF(NOPT.EQ.1)THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(NOPT.EQ.-1)RETURN + IF(NOPT.EQ.3)GO TO 200 +C INSERT, DELETE + MN = ISTART + MX = ISTART + IDIMG + POSN = 0 + CALL GETINT(MN,MX,POSN, + +'Position to edit', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + IF(IVAL.EQ.0) GO TO 1 + POSN = IVAL + POSN=POSN-ISTART+1 + MN = 1 + MX = 50 + NC = 1 + CALL GETINT(MN,MX,NC, + +'Number of characters', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + NC = IVAL + Y=POSN+NC + K=IDIMG-POSN+1 + IF((NOPT.EQ.2).AND.(Y.GT.IDIMG))NC=K +C CALC POSN IN THIS GEL TO EDIT + K=POSN +C IS THIS DELETE? + IF(NOPT.EQ.4)GO TO 100 +50 CONTINUE + LC = NC + CALL FILLC(CHARS,NC,'*') + CALL GETSTR('characters to insert',CHARS,CHARS,NC,LC,KBOUT,KBIN, + +INFLAG) + IF(INFLAG.EQ.2) GO TO 1 + IF(IFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 50 + END IF +C MOVE THE DATA RIGHT + M=IDIMG + N=IDIMG+NC +C CHECK FOR OVERFLOW OF GEL + IF(N.GT.MAXGEL)THEN + WRITE(KBOUT,2000) +2000 FORMAT(' Data pushed off end of gel during insertion') +C HOW MUCH OVER? + NM512=N-MAXGEL + N=MAXGEL +C SET M SO THAT THE DATA AT THE END IS LOST + M=M-NM512 + END IF + J=M-K+1 + DO 55 I=1,J + GEL(N)=GEL(M) + N=N-1 + M=M-1 +55 CONTINUE +C PERFORM THE INSERTION + IC=0 + DO 60 I=K,MIN(MAXGEL,K+NC-1) + IC=IC+1 +C DONT ALLOW SPACES - SET THEM TO *'S + IF(CHARS(IC).EQ.' ')CHARS(IC)='*' + GEL(I)=CHARS(IC) +60 CONTINUE + IDIMG=MIN((IDIMG+NC),MAXGEL) + GO TO 1 +CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC +C DELETE +100 CONTINUE +C +C MOVE DATA TO LEFT + M=K+NC +C POSN TO MOVE TO + N=MAX(1,K) +C NUMBER TO MOVE + L=IDIMG-M+1 +C NONE TO MOVE? + IF(L.GT.0) THEN +C MOVE THE DATA + DO 160 I=1,L + GEL(N)=GEL(M) + N=N+1 + M=M+1 +160 CONTINUE + END IF +C RESET LENGTH + IDIMG=IDIMG-NC + GO TO 1 +200 CONTINUE +C +CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC +C CHANGE + MN = ISTART + MX = ISTART + IDIMG + POSN = 0 + CALL GETINT(MN,MX,POSN, + +'Position to edit', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + IF(IVAL.EQ.0) GO TO 1 + POSN = IVAL +C ADD IN START POSITION HERE + POSN=POSN-ISTART+1 +225 CONTINUE + LC = 50 + NC = 0 + CALL GETSTR('New characters',' ',CHARS,LC,NC,KBOUT,KBIN,INFLAG) + IF(NC.EQ.0) GO TO 1 + IF(INFLAG.EQ.2) GO TO 1 + IF(IFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 225 + END IF +C CALC POSITION IN THIS GEL TO EDIT + K=POSN +C COPY CHARS + DO 230 I=1,NC + GEL(K)=CHARS(I) + K=K+1 +230 CONTINUE + GO TO 1 + END + SUBROUTINE EDR(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LGEL,NCONT, + +CON,IDC,IDEVW,IDEVR,LREG) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER CON(IDC) + INTEGER CHNRP + EXTERNAL CHNRP +C CHANGE RELATIVE POSITIONS FOR AE + ND = 0 + DO 10 I = IDC,1,-1 + IF(CON(I).EQ.'*') THEN + ND = ND + 1 + K = I + LREG - 1 + J = CHNRP(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LGEL,NCONT,K) + IF(J.NE.0) THEN + CALL SHIFTC(RELPG,LNGTHG,LNBR,RNBR,IDUM,JDUM,IDEVR, + + IDBSIZ,J,NCONT,-1) + END IF + END IF +10 CONTINUE +C WRITE(*,*)' NUMBER OF DELETIONS=',ND + END + SUBROUTINE ET(GEL,IDG,CON,IDC,K) + CHARACTER GEL(IDG),CON(IDC),CHARSL + EXTERNAL CHARSL,INDEXS + K = 0 + DO 10 I = 2,MIN(IDC,IDG) + JC = INDEXS(CON(I),J) + IF(JC.NE.6) THEN + JG = INDEXS(GEL(I),J) + IF(JG.NE.JC) THEN + JNG = INDEXS(GEL(I-1),J) + JNC = INDEXS(CON(I-1),J) + IF(JNC.NE.JNG) THEN + IF((JNG.EQ.JC).AND.(JNC.EQ.JG)) THEN + GEL(I) = CHARSL(JNG) + GEL(I-1) = CHARSL(JG) + K = K + 1 + END IF + END IF + END IF + END IF +10 CONTINUE +C WRITE(*,*)' NUMBER OF CHARS TRANSPOSED=',K + END + SUBROUTINE FDEPTH(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,LENCON, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG,DEPTHP,DEPTHM,STRAND + STRAND = 1 + CALL FDPTH(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,LENCON,STRAND,DEPTHP) + IF(DEPTHP.LT.0) RETURN + STRAND = -1 + CALL FDPTH(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,LENCON,STRAND,DEPTHM) + IF(DEPTHM.LT.0) RETURN + CALL PLTCON(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ, + +MARGL,MARGR,MARGB, + +MARGT,ISXMAX,ISYMAX,LGEL,LREG,RREG,DEPTHP,DEPTHM) + END + SUBROUTINE FDPTH(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,LENCON,STRAND,DEPTH) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG,DEPTH,STRAND + EXTERNAL NCDEP +C LREG = left contig position +C RREG = right '' '' +C LENCON = RREG-LREG+1 + I = LGEL + DEPTH = 0 +5 CONTINUE + IF(I.NE.0) THEN + IF((RELPG(I)+ABS(LNGTHG(I))-1).LT.LREG) THEN + I = RNBR(I) + GO TO 5 + END IF + ELSE + DEPTH = -1 + RETURN + END IF +C WRITE(*,*)'LGEL',LGEL +10 CONTINUE + IF(I.NE.0)THEN + IF(RELPG(I).LE.RREG) THEN + IF(SIGN(1,LNGTHG(I)).EQ.STRAND) THEN + K = RELPG(I) + ABS(LNGTHG(I)) -1 + DEPTH = MAX(NCDEP(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,I, + + STRAND,K),DEPTH) + END IF + I = RNBR(I) + GO TO 10 + END IF + END IF +C WRITE(*,*)'DEPTH',DEPTH + END +C FIND +C +C SUBROUTINE TO FIND THE FIRST OCCURENCE OF A GIVEN STRING +C IN A GIVEN ARRAY +C + SUBROUTINE FIND(SEQ,IDIM1,STRING,IDIM2,IMATCH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2),DASH + INTEGER PSEQ,PSTR + SAVE DASH + DATA DASH/'-'/ + PSEQ=0 + PSTR=1 + IMATCH=0 +C +100 CONTINUE +C +C PUT PSEQ TO WHERE THIS FAILED MATCH STARTED + PSEQ=PSEQ+1-PSTR +C +400 CONTINUE +C + PSTR=0 +C +500 CONTINUE +C +C POINT TO NEXT SEQ CHAR + PSEQ=PSEQ+1 +C TEST FOR END + IF(PSEQ.GT.IDIM1)GO TO 300 +C POINT TO NEXT STRING CHAR + PSTR=PSTR+1 +C TEST FOR DASH IN STRING + IF(STRING(PSTR).EQ.DASH)GO TO 450 +C TEST FOR DASH IN SEQ + IF(SEQ(PSEQ).EQ.DASH)GO TO 400 +C TEST FOR MATCH + IF(SEQ(PSEQ).NE.STRING(PSTR))GO TO 100 +C +450 CONTINUE +C +C TEST FOR END OF STRING IE. WHOLE STRING MATCH + IF(PSTR.LT.IDIM2)GO TO 500 +C HAVE MATCH. GET POINTER TO WHERE IT STARTED + IMATCH=PSEQ-IDIM2+1 +C +300 CONTINUE + RETURN + END + SUBROUTINE FMT4LP(SEQ1,SEQ2,IDIM,ISW,ISX,IDEV,NAME1,NAME2) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM),SEQ2(IDIM),MATCH(60),NAME1*(*),NAME2*(*) + INTEGER KL(6) + ISXX=ISX + ISWW=ISW + IE=0 +10 CONTINUE + IS=IE+1 + IE=IE+60 + IF(IE.GT.IDIM)IE=IDIM + N=IE-IS+1 + N=1+(N-1)/10 +C SET UP DECIMAL COUNTERS + DO 50 J=1,N + KL(J)=ISWW + ISWW=ISWW+10 +50 CONTINUE + WRITE(IDEV,1001)(KL(K),K=1,N) + WRITE(IDEV,1002)NAME1,(SEQ1(K),K=IS,IE) + IL = IE - IS + 1 + CALL SQMTCH(SEQ1(IS),SEQ2(IS),MATCH,IL) + WRITE(IDEV,1003)(MATCH(K),K=1,IL) + WRITE(IDEV,1002)NAME2,(SEQ2(K),K=IS,IE) +1002 FORMAT(2X,A,2X,6(10A1,1X)) + 1003 FORMAT(10X,6(10A1,1X)) +C SET UP DECIMAL COUNTERS + DO 60 J=1,N + KL(J)=ISXX + ISXX=ISXX+10 +60 CONTINUE + WRITE(IDEV,1001)(KL(K),K=1,N) +1001 FORMAT( 5X,6(I6,5X)) + IF(IE.LT.IDIM) GO TO 10 + END + SUBROUTINE FMTDB(SEQ1,IDIM,ISW,ISE,LINLEN,IDEV) +C NOTE SAME AS FMTSEP! +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM) + INTEGER KL(12) + ISWW=ISW-1 + IE=ISW-1 +1 CONTINUE + WRITE(IDEV,1003) +1003 FORMAT( ) +C SET UP DECIMAL COUNTERS + DO 50 J=1,LINLEN/10 + ISWW=ISWW+10 + KL(J)=ISWW +50 CONTINUE + IS=IE+1 + IE=IE+LINLEN + IF(IE.GT.ISE)IE=ISE + WRITE(IDEV,1001)(KL(KKK),KKK=1,MIN(IE-IS+1,LINLEN)/10) + WRITE(IDEV,1002)(SEQ1(K),K=IS,IE) +1002 FORMAT( ' ',12(10A1,1X)) +1001 FORMAT( ' ',12(5X,I6)) + IF(IE.EQ.ISE)RETURN + GO TO 1 + END + SUBROUTINE FNDCON(SEQ,IDIM,CENDS,NENDS,IDCEND,MAXCON,KBOUT) +C AUTHOR: RODGER STADEN +C STORES THEIR POSITIONS IN CENDS AND THEIR LEFT LINE NUMBERS IN NENDS + CHARACTER SEQ(IDIM),DC(3) + INTEGER CENDS(MAXCON) + INTEGER NENDS(MAXCON) + EXTERNAL IFROMC,INDEXA + IDCEND=0 + DO 10 I=1,IDIM + IF(SEQ(I).NE.'<')GO TO 10 + IDCEND=IDCEND+1 +C PUT POSITION OF LEFT END OF CONTIG IN CENDS + CENDS(IDCEND)=I + K = INDEXA(SEQ(I),20,'.') + IF(K.EQ.0) THEN + WRITE(KBOUT,*)'Error in contig title: no dot!' + IDCEND = 0 + RETURN + END IF + K = K + I +C K=I+11 + DO 5 J=1,3 + DC(J)=SEQ(K) + K=K+1 +5 CONTINUE + NENDS(IDCEND)=IFROMC(DC,3,KBOUT) +10 CONTINUE +C STORE POSITION OF LAST CHAR +1 TO SIMPLIFY DISPLAY ROUTINES + CENDS(IDCEND+1)=IDIM+1 + RETURN + END + INTEGER FUNCTION GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,IIN) +C AUTHOR: RODGER STADEN +C RETURNS CONTIG LINE NUMBER OR ZERO FOR ERROR + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + GCLIN = 0 + N=IDBSIZ-NCONTS + DO 10 J=N,IDBSIZ-1 + IF(LNBR(J).EQ.IIN) THEN + GCLIN = J + RETURN + END IF +10 CONTINUE + END + INTEGER FUNCTION GELID(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LLINO,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH,INFLAG) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN +C SEARCHES FOR ARCHIVE NAMES + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER NAME1*11,NAME2*10,NAME3*11,NFLAG + PARAMETER (NFLAG='/') + NAME3 = ' ' + IF(LLINO.NE.0) THEN + NAME3(1:1) = NFLAG + CALL READN(IDEVN,LLINO,NAME3(2:)) + END IF + GELID = 0 +10 CONTINUE + L = 0 + IF(LLINO.NE.0) L = 11 + CALL GTSTR('Contig identfier',NAME3, + +NAME1,L,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.4) RETURN + IF(INFLAG.EQ.3) THEN + GELID = LLINO + RETURN + END IF + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(NAME1(1:1).EQ.NFLAG) THEN + CALL CCASE(NAME1,1) + DO 20 I=1,NGELS + CALL READN(IDEVN,I,NAME2) + CALL CCASE(NAME2,1) + IF(NAME1(2:11).EQ.NAME2) THEN + GELID = I + RETURN + END IF +20 CONTINUE + WRITE(KBOUT,1004)NAME1(2:) +1004 FORMAT(' ',A,' is not in the database!') + ELSE + CALL RJST(NAME1) + READ(NAME1,1001,ERR=10,END=10)GELID +1001 FORMAT(I11) + IF((GELID.LT.1).OR.(GELID.GT.NGELS)) THEN + CALL ERROM(KBOUT,'Illegal gel reading number') + GO TO 10 + END IF + END IF + END + SUBROUTINE GELOUT(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ,NGELS, + +NCONTS,GEL,MAXGEL,IDEV3,IDEV4,IDEV5,IDEV1,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,FILNAM) + INTEGER RELPG(MAXDB) + INTEGER LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER GEL(MAXGEL) + CHARACTER FILNAM*(*),HELPF*(*) + CHARACTER NAMARC*10 + FILNAM = ' ' + CALL OPENF1(IDEV5,FILNAM,1,IOK,KBIN,KBOUT, + +'File for names of extracted gel readings', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + CALL YESNO(I,'Extract ends of contigs only', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(I.LT.0) RETURN + IF(I.EQ.0) GO TO 15 + DO 10 I=1,NGELS + L=ABS(LNGTHG(I)) + IF(L.GT.0)THEN + CALL READN(IDEV4,I,NAMARC) + WRITE(KBOUT,1002)NAMARC +1002 FORMAT(' ',A) + WRITE(IDEV5,1003)NAMARC +1003 FORMAT(A) + FILNAM = NAMARC + CALL OPENRS(IDEV1,FILNAM,IOK,LRECL,1) + IF(IOK.NE.0) GO TO 100 + CALL READW(IDEV3,I,GEL,MAXGEL) + IF(LNGTHG(I).LT.0)THEN + CALL SQREV(GEL,L) + CALL SQCOM(GEL,L) + END IF + CALL FMTDKN(IDEV1,GEL,L) + CLOSE(UNIT=IDEV1) + END IF +10 CONTINUE + RETURN +15 CONTINUE +C NUMBER OF LINES TO PROCESS + N=IDBSIZ-NCONTS + DO 20 I=N,IDBSIZ-1 + JL=LNBR(I) + JR=RNBR(I) + CALL READN(IDEV4,JL,NAMARC) + WRITE(KBOUT,1002)NAMARC + WRITE(IDEV5,1003)NAMARC + FILNAM = NAMARC + CALL OPENRS(IDEV1,NAMARC,IOK,LRECL,1) + IF(IOK.NE.0) GO TO 100 + CALL READW(IDEV3,JL,GEL,MAXGEL) + L=ABS(LNGTHG(JL)) + IF(LNGTHG(JL).LT.0)THEN + CALL SQREV(GEL,L) + CALL SQCOM(GEL,L) + END IF + CALL FMTDKN(IDEV1,GEL,L) + CLOSE(UNIT=IDEV1) + IF(JR.EQ.JL)GO TO 20 + CALL READN(IDEV4,JR,NAMARC) + WRITE(KBOUT,1002)NAMARC + WRITE(IDEV5,1003)NAMARC + CALL OPENRS(IDEV1,NAMARC,IOK,LRECL,1) + IF(IOK.NE.0) GO TO 100 + CALL READW(IDEV3,JR,GEL,MAXGEL) + L=ABS(LNGTHG(JR)) + IF(LNGTHG(JR).LT.0)THEN + CALL SQREV(GEL,L) + CALL SQCOM(GEL,L) + END IF + CALL FMTDKN(IDEV1,GEL,L) + CLOSE(UNIT=IDEV1) +20 CONTINUE + RETURN +100 CONTINUE + WRITE(KBOUT,*)'Error opening file for extracted gel reading' + RETURN + END + SUBROUTINE GETLN2(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LINCON,LLINO,IGELNO,IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),GELID + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER FILEH*(*) + EXTERNAL GELID + IERR = 1 + NCONTC = GELID(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LLINO, + +IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH,INFLAG) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.4) RETURN + IF(NCONTC.EQ.0) RETURN + IGELNO = NCONTC + IF(LNBR(NCONTC).NE.0) THEN + WRITE(KBOUT,1013)RELPG(NCONTC) +1013 FORMAT(' Position of this reading=',I6) +25 CONTINUE + NCONTC = LNBR(NCONTC) + IF(LNBR(NCONTC).NE.0) GO TO 25 + WRITE(KBOUT,1014)NCONTC +1014 FORMAT( ' Number of leftmost reading this contig=',I6) + END IF +30 CONTINUE + N = IDBSIZ - NCONTS + DO 20 J=N,IDBSIZ-1 + IF(LNBR(J).EQ.NCONTC) THEN + LINCON=J + GO TO 21 + END IF +20 CONTINUE + WRITE(KBOUT,9999) +9999 FORMAT(' No contig line for this gel! Fix the database') + RETURN +21 CONTINUE + LLINO = NCONTC + IERR = 0 + END + SUBROUTINE GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LINCON,LLINO,IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) + CALL GETLN2(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LINCON,LLINO,IGELNO,IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) + END + SUBROUTINE GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LEFTMN,RIGHTM,LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER LREG,RREG,RIGHTM + CHARACTER FILEH*(*) +40 CONTINUE + MN = LEFTMN + MX = RIGHTM + LREG = MN + CALL GETINT(MN,MX,LREG, + +'Start position in contig', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + LREG = IVAL + MN = LREG + MX = RIGHTM + RREG = MX + CALL GETINT(MN,MX,RREG, + +'End position in contig', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + RREG = IVAL +C NOW FIND FIRST GEL THAT OVER LAPS +50 CONTINUE + X=RELPG(LLINO)+(ABS(LNGTHG(LLINO)))-1 + IF(X.GE.LREG)GO TO 60 +C NOT IN REGION + LLINO=RNBR(LLINO) + GO TO 50 +60 CONTINUE + RETURN + END + SUBROUTINE GLEVEL(T,YF,YT,Y0,YP1,YP2,YM1,YM2) + CHARACTER T + IF(T.EQ.'0') THEN + YF = Y0 + YT = Y0 + ELSE IF(T.EQ.'1') THEN + YF = Y0 + YT = YM1 + ELSE IF(T.EQ.'2') THEN + YF = Y0 + YT = YP1 + ELSE IF(T.EQ.'3') THEN + YF = YP1 + YT = YM1 + ELSE IF(T.EQ.'4') THEN + YF = YP2 + YT = YM2 + END IF + END + CHARACTER*1 FUNCTION GTCONC(COUNTS,IDM,CUT) + INTEGER IDM + INTEGER COUNTS(IDM) + CHARACTER CHARSU + EXTERNAL CHARSU +C 8-4-92 made this routine sum counts + GTCONC = '-' + ISUM = 0 + DO 5 I=1,IDM + ISUM = ISUM + COUNTS(I) + 5 CONTINUE + IF(ISUM.EQ.0.) RETURN + Y = ISUM + DO 10 I = 1,IDM - 1 + X = REAL(COUNTS(I))/Y + IF(X.GE.CUT) THEN + GTCONC = CHARSU(I) + RETURN + END IF +10 CONTINUE + END + SUBROUTINE HIGHLT(GELSAV,NAMSAV,NUMSAV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IDEV1,IDEV2, + +FILNAM,IOK) + CHARACTER LINEIN*119,CONSEN*100 + CHARACTER GELNO*4,GEL*100,GELSAV*100,GELNAM*10 + CHARACTER NAMSAV*10,NUMSAV*4 + CHARACTER FILNAM*(*),HELPF*(*) + DIMENSION GELSAV(50),NAMSAV(50),NUMSAV(50) + CHARACTER PLUS*4,MINUS*4 + EQUIVALENCE (LINEIN(2:2),GELNO),(LINEIN(8:8),GELNAM) + EQUIVALENCE (LINEIN(20:20),GEL) + EXTERNAL NOTIRL + CALL OPENF1(IDEV1,FILNAM,0,IOK,KBIN,KBOUT, + +'File containing contig display', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,1,IOK,KBIN,KBOUT, + +'File for problem display', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN +C +C FORMAT: +C +C12345678901234567890 10 20 30 ETC +C 12 GELNAM0000 CAGACGCGCGCGCGCGCGGATATAGTCTCTCCGCTCT +C 100 GELNAM0000 TGATACGCTCGCTCTCTCTCTCTCTCTCTTTC +C AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA +C +C 70 80 ETC +C 12 GELNAM0000 AAAAAAAAAAAAAAAAAAAAAAAAAAAA +C +C + LIN = 1 + CALL GTSTR('plus strand symbol',':',PLUS,LIN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + IF(LIN.EQ.0) PLUS = ':' + LIN = 1 + CALL GTSTR('minus strand symbol','.',MINUS,LIN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + IF(LIN.EQ.0) MINUS = '.' +C COUNT LINE NUMBERS + LINNO=0 +10 CONTINUE +C READ LINE OF NOS + READ(IDEV1,1003,END=100)LINEIN + LINNO=LINNO+1 +1003 FORMAT(A) +C WRITE IT OUT AGAIN + WRITE(IDEV2,1003)LINEIN +C ZERO GEL COUNT FOR THIS STRIP + IGEL=0 +20 CONTINUE +C +C READ A LINE, COULD BE 1 GEL, 2 CONSENSUS OR BLANK +C LINEIN=' ' + READ(IDEV1,1003,END=100)LINEIN + LINNO=LINNO+1 +C WHAT SORT OF LINE? ONLY A GEL WILL HAVE NON BLANK CHARS AT THE LEFT END + IF(LINEIN(2:5).NE.' ')THEN +C GEL LINE SO SAVE + IGEL=IGEL+1 + GELSAV(IGEL)=GEL + NAMSAV(IGEL)=GELNAM + NUMSAV(IGEL)=GELNO + GO TO 20 + END IF +C MUST BE CONSENSUS + CONSEN=GEL +C PROCESS THIS STRIP OF GELS (IGEL OF THEM) + DO 50 I=1,IGEL +C WHERE DOES DATA START AND END? + IFIRST=1 +40 CONTINUE + IF(GELSAV(I)(IFIRST:IFIRST).NE.' ')GO TO 45 + IFIRST=IFIRST+1 + IF(IFIRST.LE.100)GO TO 40 +C ERROR --- NO DATA FOUND + WRITE(KBOUT,1004)LINNO +1004 FORMAT(' Error on line',I6,' of file') + RETURN +45 CONTINUE +C NOW WHERE DOES IT END + ILAST=NOTIRL(GELSAV(I),100,' ') +C COMPARE WITH CONSENSUS + READ(NUMSAV(I),'(I4)',ERR=900)INTEG + IF(INTEG.GE.0)CALL IDTOD(CONSEN,GELSAV(I),IFIRST,ILAST,PLUS) + IF(INTEG.LT.0)CALL IDTOD(CONSEN,GELSAV(I),IFIRST,ILAST,MINUS) + WRITE(IDEV2,1008)NUMSAV(I),NAMSAV(I),GELSAV(I)(1:ILAST) +1008 FORMAT(' ',A,2X,A,2X,A) +50 CONTINUE + WRITE(IDEV2,1009)CONSEN +1009 FORMAT(' ',18X,A) +1006 FORMAT( ) +C READ A BLANK LINE + READ(IDEV1,1003,END=100)LINEIN + LINNO=LINNO+1 + WRITE(IDEV2,1003)LINEIN +C NO GO BACK FOR THE NEXT LINE OF NUMBERS + GO TO 10 +100 CONTINUE + WRITE(KBOUT,1005) +1005 FORMAT(' Finished') + RETURN +900 WRITE(KBOUT,*)'Error reading gel number' + END + SUBROUTINE IDPLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ, + +NCONTS,IX,IY,MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX, + +DBTDUX,DBTDUY,NCONT,IGEL,IS) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER CHNRP1 + EXTERNAL CWORLD,CHNRP1 + YMAX = ISYMAX + YMIN = 0. + XMIN = 0. + LENCON = 0 + DO 10 I = IDBSIZ-NCONTS,IDBSIZ-1 + LENCON = LENCON + RELPG(I) +10 CONTINUE + XMAX = LENCON + XX = CWORLD(IX,MARGL,MARGR,XMIN,XMAX) + YX = CWORLD(IY,MARGB,MARGT,YMIN,YMAX) + YINC = (YMAX-YMIN)/3. + Y = 0. + XF = XMIN + N = 0 + DO 20 I = IDBSIZ-NCONTS,IDBSIZ-1 + N = N + 1 + XT = XF + RELPG(I) + Y = Y + YINC + IF((XX.GT.XF).AND.(XX.LT.XT)) THEN + IS = NINT(((XX-XF)/(XT-XF)) * RELPG(I)) + JGEL = LNBR(I) + IGEL = CHNRP1(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,JGEL,IS) + NCONT = I + RETURN + END IF + XF = XT + IF(N.EQ.2) THEN + N = 0 + Y = 0. + END IF +20 CONTINUE + IGEL = 0 + NCONT = 0 + END + SUBROUTINE IDTOD(TOPLIN,GEL,IFIRST,ILAST,SYMBOL) + CHARACTER TOPLIN*100,GEL*100,SYMBOL*4 + DO 10 I=IFIRST,ILAST + IF(GEL(I:I).EQ.TOPLIN(I:I))GEL(I:I)=SYMBOL(1:1) +10 CONTINUE + END + INTEGER FUNCTION INDEXS(C,S) + PARAMETER (IDM = 29) + CHARACTER C + INTEGER POINTS(0:255),SCORES(IDM),IND(IDM),S + COMMON /SHOTC/POINTS + SAVE /SHOTC/ + SAVE SCORES,IND + DATA + +IND/1,2,3,4,1,2,3,4,1,2,3,4,1,2,3,4,6,6,6,6,6,6,1,2,3,4,5,5,6/ +C DATA DUP/'CTAG1234DVBHKLMNRY5678ctag*,-'/ +C changed 28-7-91 to give 10 to old zeroes and 100 to lowercase + DATA SCORES/ + +100,100,100,100, + +75,75,75,75, + +100,100,100,100, + +100,100,100,100, + +10,10,10,10,10,10, + +100,100,100,100,100,100,10/ + I = ICHAR(C) + I = POINTS(I) + S = SCORES(I) + INDEXS = IND(I) + END +C ROUTINES TO CONTROL CHARACTER LOOKUP FOR SHOTGUN SEQUENCING + SUBROUTINE INITS +C AUTHOR RODGER STADEN + INTEGER POINTS(0:255) + PARAMETER (IDM = 29) + CHARACTER DUP*29 + COMMON /SHOTC/POINTS + SAVE /SHOTC/ + DATA DUP/'CTAG1234DVBHKLMNRY5678ctag*,-'/ +C ICHAR RETURNS THE COLLATING SEQUENCE NUMBER +C I WANT 1-4 FOR ACGT +C acgt +C 1234 +C BDHV +C KLMN +C 5 FOR * +C 6 FOR 5678- AND ELSE +C THE ACTUAL VALUE RETURNED BY ICHAR IS NOT PORTABLE +C SO I NEED TO INITIALIZE POINTR SO THAT THE CORRECT +C ELEMENTS CONTAIN VALUES 1 - 6 +C + DO 30 I = 0,255 + POINTS(I) = IDM +30 CONTINUE + DO 35 I = 1,IDM + J = ICHAR(DUP(I:I)) + POINTS(J) = I +35 CONTINUE + END + FUNCTION INLIST(LIST,IDLIST,ITEM) +C AUTHOR: RODGER STADEN +C SENT LIST LIST, AND ITEM ITEM. IF IN LIST RETURNS ELEMENT NUMBER, ELSE 0 + INTEGER LIST(IDLIST) + INLIST=0 + DO 1 I=1,IDLIST + IF(LIST(I).NE.ITEM)GO TO 1 + INLIST=I + RETURN +1 CONTINUE + RETURN + END + SUBROUTINE IPLTC(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,STRAND, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,YMID,YINC,DEPTH,X,Y,KBOUT, + +IGEL,IOK) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG,STRAND,DEPTH + IOK = 1 + XMIN = LREG + XMAX = RREG + YMAX = ISYMAX + YMIN = 0. + YINCO2 = STRAND*YINC/2. + I = LGEL + IGEL = 0 +5 CONTINUE + IF(I.NE.0) THEN + IF((RELPG(I)+ABS(LNGTHG(I))-1).LT.LREG) THEN + I = RNBR(I) + GO TO 5 + END IF + END IF + N = 0 +10 CONTINUE + IF(I.NE.0)THEN + IF(RELPG(I).LE.RREG) THEN + IF(SIGN(1,LNGTHG(I)).EQ.STRAND) THEN + XF = MAX(RELPG(I),LREG) + XT = MIN(ABS(LNGTHG(I))+RELPG(I)-1,RREG) + N = N + 1 + IF(N.GT.DEPTH) N = 1 + YF = YMID + N * YINC + IF((X.GE.XF).AND.(X.LE.XT)) THEN + IGEL = I + IF((Y.GE.YF-YINCO2).AND.(Y.LE.YF+YINCO2)) THEN + IOK = 0 + RETURN + END IF + END IF + END IF + I = RNBR(I) + GO TO 10 + END IF + END IF + END + SUBROUTINE JOIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LNCONL,LLINOL,LNCONR,LLINOR,GEL,GEL2, + +IDBSIZ,IDEV,KBIN,KBOUT,IDEVR,IDEVW,IDEVN,LINLEN,PERCD, + +HELPS1,HELPE1,HELPS2,HELPE2,FILEH,IDEVH,MAXGEL,IDM) + CHARACTER FILEH*(*) + INTEGER HELPS1,HELPE1,HELPS2,HELPE2 +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),ANS + INTEGER LREG,RREG,X,RELX,RIGHTM + INTEGER LNGTHG(IDBSIZ),RNBR(IDBSIZ),LNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),GEL2(MAXGEL) + PARAMETER (MAXPRM = 32) + CHARACTER PRMPT(5)*(MAXPRM) +15 CONTINUE + WRITE(KBOUT,1001) +1001 FORMAT( + +' The joint is the position in the left contig that',/, + +' the first character of the right contig overlaps.') + MN = 1 + MX = RELPG(LNCONL) + RELX = 0 + CALL GETINT(MN,MX,RELX, + +'Position of join', + +IVAL,KBIN,KBOUT,HELPS1,HELPE1,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF(IVAL.EQ.0) RETURN + RELX = IVAL + I1=1 + I2=LINLEN + IF((RELPG(LNCONR)).LT.I2)I2=(RELPG(LNCONR)) + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR,GEL2, + +LLINOL,LNCONL,RELX,RELPG(LNCONL),GEL,I1,I2,3,LLINOR, + +IDBSIZ,IDEV,KBOUT,IDEVW,IDEVN,LINLEN,PERCD,MAXGEL,IDM) +30 CONTINUE + CALL BELL(1,KBOUT) +C CALL DBMENU(3,NOPT,HELPS1,HELPE1,FILEH,IDEVH, +C +KBIN,KBOUT) + PRMPT(1) = 'Complete join' + PRMPT(2) = 'Edit left contig' + PRMPT(3) = 'Display join' + PRMPT(4) = 'Edit right contig' + PRMPT(5) = 'Move join' + NOPT = 1 + CALL RADION('Join options', PRMPT, 4, NOPT, IHELPS, + + IHELPE, FILEH, IDEVH, KBIN, KBOUT) + IF(NOPT.LT.1)RETURN +100 CONTINUE + IF(NOPT.EQ.5)GO TO 15 + IF(NOPT.EQ.2)THEN +C EDIT LEFT CONTIG + IMAXL=RELX + IMAXR=MIN(RELPG(LNCONL),(RELX+RELPG(LNCONR)-1)) + CALL DBEDIT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,GEL2, + + LNCONL,IMAXL,IMAXR,IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW, + + HELPS2,HELPE2,FILEH,IDEVH,MAXGEL) + END IF +200 CONTINUE + IF(NOPT.EQ.4)THEN +C EDIT RIGHT CONTIG + IMAXL=1 + IMAXR=MIN((RELPG(LNCONL)-RELX+1),RELPG(LNCONR)) + CALL DBEDIT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,GEL2, + + LNCONR,IMAXL,IMAXR,IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW, + + HELPS2,HELPE2,FILEH,IDEVH,MAXGEL) + END IF +300 CONTINUE + IF(NOPT.EQ.3)THEN +C DISPLAY + LLINO=LLINOL + LEFTMN=RELX + RIGHTM=RELPG(LNCONL) + IF((RIGHTM-LEFTMN+1).GT.RELPG(LNCONR))RIGHTM= + + LEFTMN+RELPG(LNCONR)-1 + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + LEFTMN,RIGHTM,LREG,RREG, + + LNCONL,LLINO,IDBSIZ,KBIN,KBOUT, + + HELPS1,HELPE1,FILEH,IDEVH,IERR) + IF(IERR.NE.0) GO TO 30 + I1=(LREG-RELX)+1 + I2=(RREG-RELX)+1 + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR,GEL2,LLINO, + + LNCONL,LREG,RREG,GEL,I1,I2,3,LLINOR,IDBSIZ,IDEV,KBOUT, + + IDEVW,IDEVN,LINLEN,PERCD,MAXGEL,IDM) + END IF +40 CONTINUE + IF(NOPT.EQ.1)THEN +C COMPLETE JOIN + CALL YESNO(ANS,'Sure', + + HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) RETURN + IF(ANS.NE.0) GO TO 30 +C ADJUST ALL RELATIVE POSITIONS IN RIGHT CONTIG + N=LLINOR + RELPG(N)=RELX +50 CONTINUE + IF(RNBR(N).EQ.0)GO TO 60 + N=RNBR(N) + RELPG(N)=RELPG(N)+RELX-1 + GO TO 50 +60 CONTINUE +C +C FIX UP NEW GEL LINE FOR OLD LEFT OF RIGHT CONTIG + LNBR(LLINOR)=RNBR(LNCONL) +C FIX UP RIGHT GEL OF LEFT CONTIG + N=RNBR(LNCONL) + RNBR(N)=LLINOR + CALL MERGE(RELPG,LNGTHG,LNBR,RNBR,LNCONL,IDBSIZ) +C MERGE DOES NOT WRITE TO DISK + N=LNBR(LNCONL) +65 CONTINUE + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) + N=RNBR(N) + IF(N.NE.0)GO TO 65 +C CONTIG LINES + X=RELPG(LNCONR)+RELX-1 + IF(X.GT.RELPG(LNCONL))RELPG(LNCONL)=X + CALL WRITER(IDEVR,LNCONL,RELPG(LNCONL),LNGTHG(LNCONL), + +LNBR(LNCONL),RNBR(LNCONL)) +C NOW MOVE ALL DATA DOWN TO DELETE OLD RIGHT END + N=IDBSIZ-NCONTS + M=LNCONR-N + IF(M.EQ.0)GO TO 80 + K=LNCONR + J=LNCONR-1 + DO 70 I=1,M + RELPG(K)=RELPG(J) + LNGTHG(K)=LNGTHG(J) + LNBR(K)=LNBR(J) + RNBR(K)=RNBR(J) + CALL WRITER(IDEVR,K,RELPG(K),LNGTHG(K), + +LNBR(K),RNBR(K)) + K=K-1 + J=J-1 +70 CONTINUE +80 CONTINUE + NCONTS=NCONTS-1 + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + RETURN + END IF + GO TO 30 + END +C LINEUP +C +C TAKES 2 SEQS SET OF MATCHES AND PRODUCES LINED UP SEQS +C FINDS IF WE HAVE A LEFT OVERLAP +C RETURNS POSITION OF JOINT. THIS IS RELATIVE TO THE CONTIG +C FOR MOST MATCHES BUT I RELATIVE TO THE GEL FOR A LEFT OVERLAP + SUBROUTINE LINEUP(SEQG,SEQC,SEQG2,SEQC2,IDC,IDG,IDOUT, + 1MATG,MATC,MATL,IP,ITOTPC,ITOTPG,JOINT,ITYPE,KBOUT,MAXGEL,IFAIL) +C AUTHOR: RODGER STADEN + CHARACTER SEQG(IDG),SEQC(IDC),SEQG2(IDOUT),SEQC2(IDOUT),PAD + INTEGER MATG(IP),MATC(IP),MATL(IP) + SAVE PAD + DATA PAD/','/ + IFAIL=0 +C ZERO PADDING CHARS IN CONTIG (GEL DONE AT END BY DIFFERENCE +C IN INPUT AND OUTPUT LENGTHS) + ITOTPC=0 +C FILL OUTPUT WITH PADDING + DO 10 I=1,IDOUT + SEQG2(I)=PAD + SEQC2(I)=PAD +10 CONTINUE + NMTCH=0 +C SET INITIAL POINTERS TO OUTPUT +C CONSENSUS + IS1=1 +C GEL + IS2=1 +C FIND DISTANCE FROM LEFT MATCH IN GEL TO LEFT OF GEL + IG2=MATG(1)-1 + IF(IG2.EQ.0)THEN +C THE LEFT END OF THE GEL MATCHES SO THIS IS NOT A LEFT OVERLAP +C SET TYPE + ITYPE=-1 +C SET JOINT + JOINT=MATC(1) +C SKIP NEXT SECTION + GO TO 50 + END IF +C FIND DISTANCE FROM LEFT MATCH IN CONTIG TO LEFT OF CONTIG + IC2=MATC(1)-1 +C GET DISTANCE FROM FIRST MATCH IN CONTIG TO FIRST MATCH IN GEL. +C IF THIS DISTANCE <0 THEN WE HAVE A LEFT OVERLAP + IC1=IC2-IG2+1 + IF(IC1.GT.0)THEN +C THIS IS NOT A LEFT OVERLAP +C SET TYPE + ITYPE=-1 +C SET LEFT END + JOINT=IC1 +C COPY THE GEL UPTO THE FIRST MATCH, INTO THE OUTPUT ARRAY +C CHECK FOR OVERFLOW + IF(IG2.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQG(1),SEQG2(1),IG2) +C COPY THE CONTIG FOR THE SAME REGION + IF(IG2.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQC(IC1),SEQC2(1),IG2) + IS1=IS1+IG2 + IS2=IS2+IG2 + GO TO 50 + END IF +C MUST BE LEFT END OVERLAP +C SET TYPE + ITYPE=1 +C SET POSITION OF JOINT RELATIVE TO GEL + JOINT=ABS(IC1)+2 +C COPY OVER THE GEL UPTO THE JOINT +C CHECK FOR OVERFLOW + IF(IG2.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQG(1),SEQG2(1),IG2) + IS2=IS2+IG2 +C WE MAY ALSO HAVE MISMATCHING +C DATA AT THE JOIN SO DEAL WITH THAT NOW +C IF IC2 >0 THE LEFT END OF THE CONTIG MATCHES THE GEL BUT OTHERWISE +C WE HAVE SOME MISMATCHED DATA TO DEAL WITH - WE NEED TO TRANSFER +C THE MISMATCHED REGION OF THE CONTIG TO THE OUTPUT ARRAY + IF(IC2.GT.0)THEN + IF(IC2.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQC(1),SEQC2(1),IC2) + IS1=IS1+IC2 + END IF +C WHEN WE GET HERE WE HAVE SORTED OUT THE LEFT ENDS FOR LEFT OVERLAP +C AND MISMATCHED LEFT ENDS, WE NOW DEAL WITH THE REST OF THE SEQUENCE +C STARTING WITH THE FIRST BLOCK OF IDENTITY +C +C IG1 POSITION IN INPUT GEL +C IS2 POSITION IN OUTPUT GEL +C IC1 POSITION IN INPUT CONTIG +C IS1 POSITION IN OUTPUT CONTIG +C LG1 POSITION OF END OF CURRENT MATCH IN OUTPUT GEL +C LC1 POSITION OF END OF CURRENT MATCH IN OUTPUT CONTIG +C LG2 DISTANCE FROM CURRENT MATCH IN INPUT GEL TO NEXT MATCH +C LC2 DISTANCE FROM CURRENT MATCH IN INPUT CONTIG TO NEXT MATCH +C +50 CONTINUE +C POINT TO NEXT MATCH + NMTCH=NMTCH+1 +C COPY NEXT MATCH + IG1=MATG(NMTCH) + IC1=MATC(NMTCH) + L=MATL(NMTCH) +C CHECK FOR OVERFLOW + IF(IS2+L-1.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQG(IG1),SEQG2(IS2),L) +C CHECK FOR OVERFLOW + IF(IS1+L-1.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQC(IC1),SEQC2(IS1),L) +C POINT TO NEXT OUTPUT POSITIONS + IS1=IS1+L + IS2=IS2+L +C END OF CURRENT MATCH + LG1=IG1+L + LC1=IC1+L +C ANY MORE MATCHES + IF(NMTCH.EQ.IP)GO TO 500 + K=NMTCH+1 + LG2=MATG(K)-LG1 + LC2=MATC(K)-LC1 +C ANY DIFFERENCE IN LENGTH? IF SO WE HAVE TO PAD SO THEY BECOME THE SAME + L5=ABS(LG2-LC2) +C COUNT PADDING CHARS IN CONTIG + IF(LG2.GT.LC2)ITOTPC=ITOTPC+L5 +C IF DIFFERENCE INCREMENT SHORTER + IF(LG2.GT.LC2)IS1=IS1+L5 +C IF GEL NEEDS PADDING TRY TO PUT PADS NEXT TO DOUBLE CODES + IF(LC2.GT.LG2)CALL PADCOP(SEQG,SEQG2, + +LG1,MATG(K),L5,IS2,LG2,MAXGEL,IFAIL,KBOUT,SEQC,LC1) +C CHECK FOR OVERFLOW + IF(IFAIL.EQ.1)GO TO 700 +C NOW COPY MISSMATCHED REGION +C CHECK FOR OVERFLOW + IF(IS2+LG2-1.GT.MAXGEL)GO TO 700 + IF(LG2.GT.0)CALL SQCOPY(SEQG(LG1),SEQG2(IS2),LG2) +C CHECK FOR OVERFLOW + IF(IS1+LC2-1.GT.MAXGEL)GO TO 700 + IF(LC2.GT.0)CALL SQCOPY(SEQC(LC1),SEQC2(IS1),LC2) +C POINT TO NEXT OUTPUT POSITIONS + IS1=IS1+LC2 + IS2=IS2+LG2 +C GET NEXT MATCH + GO TO 50 +500 CONTINUE +C +C FINISH RIGHT ENDS +C ONLY COPY TO END OF GEL IN GEL AND TO THE SAME RELATIVE POSITION +C IN THE CONTIG FOR DISPLAY PURPOSES AND FOR COUNTING MISMATCH +C CURRENT ENDS AT LG1,LC1 +C HOW FAR TO END OF GEL? +C SET M + M=0 + L=IDG-LG1+1 + IF(L.LT.1)GO TO 600 +C CHECK FOR OVERFLOW + IF(IS2+L-1.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQG(LG1),SEQG2(IS2),L) +C NEED TO COPY TO END OF GEL IN CONTIG FOR DISPLAY +C POINT TO POSN IN CONTIG LEVEL WITH END OF GEL + M=LC1+L-1 +C IS THIS OVER END OF CONTIG? + IF(M.GT.IDC)M=IDC +C NUMBER TO COPY + M=M-LC1+1 +C CHECK FOR OVERFLOW + IF(IS1+M-1.GT.MAXGEL)GO TO 700 + IF(M.GT.0)CALL SQCOPY(SEQC(LC1),SEQC2(IS1),M) +600 CONTINUE +C COUNT PADDING IN GEL + ITOTPG=IS2+L-1-IDG +C SET NEW LENGTHS FOR RETURN TO CALLING ROUTINE + IDOUT=IS1+M-1 + IDG=IS2+L-1 + IFAIL=0 + RETURN +700 CONTINUE + WRITE(KBOUT,1000) +1000 FORMAT(' Matching region too long for routine lineup,', + +' alignment aborted') + IFAIL=1 + RETURN + END + SUBROUTINE LSTCON(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LLINO, + +RREG,IDEV,IDEVN,NAMARC) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG + CHARACTER NAMARC*(*) + N = LLINO + WRITE(IDEV,1001) +10 CONTINUE + CALL READN(IDEVN,N,NAMARC) + WRITE(IDEV,1006)NAMARC,N,RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) + IF(RNBR(N).NE.0) THEN + N = RNBR(N) + IF(RELPG(N).LE.RREG) GO TO 10 + END IF +1001 FORMAT(' NAME NUMBER POSITION LENGTH NEIGHBOURS'/ + +' LEFT RIGHT') +1006 FORMAT( ' ',A,2X,I4,2X,I7,2X,I5,2X,I6,2X,I6) + END +C12345678901234567890 +C 710 720 730 740 750 +C -1 HINW.004 CGTCAGACGCACGCTGGAAAA + INTEGER FUNCTION LTYPE(LINE,LL,J1,J2,N,MAXDB,KBOUT) + CHARACTER LINE*(*),NUM*5,SPACE + EXTERNAL NOTRL,NOTLR + PARAMETER (SPACE= ' ') + J1 = NOTLR(LINE,LL,SPACE) + IF(J1.EQ.0) THEN +C BLANK LINE + LTYPE = 1 + RETURN + END IF + IF(J1.GT.20) THEN +C LINE OF NUMBERS + LTYPE = 2 + RETURN + END IF + IF(J1.GT.5) THEN +C CONSENSUS LINE + LTYPE = 3 + RETURN + END IF +C SHOULD BE A SEQUENCE LINE + J = INDEX(LINE(J1:),SPACE) + NUM = SPACE + NUM = LINE(J1:J1+J-2) + CALL RJST(NUM) +C N = IFROMS(NUM,5,KBOUT) + READ(NUM,'(I5)',ERR=10) N + IF(N.GT.MAXDB-2) GO TO 10 +C NUMBER ENDS AT J1+J-2 + J1 = J1 + J - 1 +C LOOK FOR BEGINNING OF NAME + J = NOTLR(LINE(J1:),LL-J1+1,SPACE) + N1 = J1 + J - 1 +C LOOK FOR END OF NAME + J = INDEX(LINE(N1:),SPACE) + N2 = N1 + J - 2 +C LOOK FOR BEGINNING OF SEQ + J = NOTLR(LINE(N2+1:),LL-N2,SPACE) + J1 = N2 + J + LTYPE = 4 +C LOOK FOR END OF SEQ + J2 = NOTRL(LINE,LL,SPACE) + IF(J2.GT.N2) RETURN +10 CONTINUE + LTYPE = 0 + END + INTEGER FUNCTION LWRAPS(I,J) + K = MOD(I,J) + IF(K.EQ.0) K = J + LWRAPS = K + END +C MERGE +C +C ROUTINE SENT CONTIG WHOSE GELS MAY BE OUT OF ORDER +C REORDERS GELS ON POSITION OF LEFT ENDS AND SETS LEFT +C GEL NUMBER FOR THE REORDERED CONTIG +C + SUBROUTINE MERGE(RELPG,LNGTHG,LNBR,RNBR,LINCON,IDBSIZ) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) +C +C START AT LEFT END + N=LNBR(LINCON) + GO TO 22 +21 CONTINUE +C SET POINTER TO NEXT GEL TO RIGHT IN LIST + N=NR + IF(I1.GT.0)N=I2 +22 CONTINUE +C SET POINTER TO NEXT GEL TO RIGHT + NR=RNBR(N) + IF(NR.EQ.0)GO TO 30 +C HAVENT REACHED END YET + I1=0 +23 CONTINUE +C ARE THESE 2 IN CORRECT ORDER IE N<=NR ? + IF(RELPG(N).LE.RELPG(NR))GO TO 21 +C NOT IN ORDER SO CHAIN LEFT UNTIL CORRECTLY POSITIONED +C THEN COME BACK TO THIS POINT AND CONTINUE +C IF FIRST MOVE SAVE POSITION + IF(I1.EQ.0)I2=N + I1=1 +C EXCHANGE NEIGHBOURS + M=RNBR(NR) + IF(M.NE.0)LNBR(M)=N + M=LNBR(N) + IF(M.NE.0)RNBR(M)=NR + RNBR(N)=RNBR(NR) + RNBR(NR)=N + LNBR(NR)=LNBR(N) + LNBR(N)=NR +C CHAIN BACK THRU LIST + N=LNBR(NR) + IF(N.EQ.0)GO TO 21 +C END NOT REACHED + GO TO 23 +30 CONTINUE +C ALL DONE POINTER AT RIGHT GEL + RNBR(LINCON)=N + RETURN + END + SUBROUTINE MINCOM(SEQ1,IDIM1,SEQ2,IDIM2,SAV1,SAV2,SAV3, + +IP,MINM,KBOUT) +C AUTHOR: RODGER STADEN +C + CHARACTER SEQ1(IDIM1),SEQ2(IDIM2) + INTEGER SAV1(IP),SAV2(IP),SAV3(IP) +C + IP1=IP + IP=0 +C +C SITUATION 1 + NT1=IDIM2-MINM + IES1=MINM-1 + ISS2=NT1+1 +C + DO 100 I=1,NT1 +C +C POINT TO FIRST CHAR-1 OF SEQ2 + ISS2=ISS2-1 +C POINT TO LAST CHAR SEQ1 + IES1=IES1+1 +C + N=0 +C + DO 200 J=1,IES1 +C STORE POINTER + JJ=J +C +C POINT TO SEQ2 + K=ISS2+J +C TEST FOR EQUALITY + IF(SEQ1(J).NE.SEQ2(K))GO TO 220 +C INCREMENT N + N=N+1 + GO TO 200 +220 CONTINUE +C TEST FOR SUFFICENTLY LARGE N + IF(N.GE.MINM)CALL SAVIT(N,J,K,IP,SAV1,SAV2,SAV3,IP1) +C TEST FOR OVERFLOW + IF(IP.GT.IP1)GO TO 5000 +C RESET N TO ZERO + N=0 +200 CONTINUE +C +C GOOD SCORE AT END? +C NEED TO INCREMENT POINTERS AS SAVIT EXPECTS TO BE POINTING AT NEXT +C MISMATCH + JJ=JJ+1 + KK=K+1 + IF(N.GE.MINM)CALL SAVIT(N,JJ,KK,IP,SAV1,SAV2,SAV3,IP1) +C TEST FOR OVERFLOW + IF(IP.GT.IP1)GO TO 5000 +C +100 CONTINUE +C +C +C SITUATION 2 + NT2=IDIM1-IDIM2+1 +C + DO 300 I=1,NT2 + N=0 +C + DO 400 J=1,IDIM2 +C SAVE POINTER + JJ=J +C +C SET POINTER TO SEQ1 + L=I+J-1 + IF(SEQ1(L).NE.SEQ2(J))GO TO 420 + N=N+1 + GO TO 400 +420 CONTINUE + IF(N.GE.MINM)CALL SAVIT(N,L,J,IP,SAV1,SAV2,SAV3,IP1) +C TEST FOR OVERFLOW + IF(IP.GT.IP1)GO TO 5000 + N=0 +400 CONTINUE + LL=L+1 + JJ=JJ+1 + IF(N.GE.MINM)CALL SAVIT(N,LL,JJ,IP,SAV1,SAV2,SAV3,IP1) +C TEST FOR OVERFLOW + IF(IP.GT.IP1)GO TO 5000 +300 CONTINUE +C +C +C SITUATION 3 + ISS1=IDIM1-IDIM2 +C + DO 500 I=1,NT1 +C +C POINT TO FIRST CHAR SEQ1 + K=ISS1+I + IES2=IDIM2-I + N=0 +C + DO 600 J=1,IES2 +C SAVE POINTER + JJ=J +C +C POINT TO SEQ1 + L=K+J + IF(SEQ1(L).NE.SEQ2(J))GO TO 620 + N=N+1 + GO TO 600 +620 CONTINUE + IF(N.GE.MINM)CALL SAVIT(N,L,J,IP,SAV1,SAV2,SAV3,IP1) +C TEST FOR OVERFLOW + IF(IP.GT.IP1)GO TO 5000 + N=0 +600 CONTINUE +C + LL=L+1 + JJ=JJ+1 + IF(N.GE.MINM)CALL SAVIT(N,LL,JJ,IP,SAV1,SAV2,SAV3,IP1) +C TEST FOR OVERFLOW + IF(IP.GT.IP1)GO TO 5000 +500 CONTINUE +C + RETURN +5000 CONTINUE +C OVERFLOW +C + WRITE(KBOUT,1000)IP1 +1000 FORMAT(/' TOO MANY MATCHES. LIMIT = ',I6) + RETURN + END + SUBROUTINE ML(PC,PG,L,N,J) + INTEGER PC(N),PG(N),L(N) + DO 10 I = J,N-1 + PC(I) = PC(I+1) + PG(I) = PG(I+1) + L(I) = L(I+1) +10 CONTINUE + END + SUBROUTINE MSTLKL(SEQ,IDIM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + CHARACTER CHARSU + EXTERNAL CHARSU,INDEXS + DO 100 I=1,IDIM + J = INDEXS(SEQ(I),K) + SEQ(I) = CHARSU(J) +100 CONTINUE + END + CHARACTER FUNCTION MUNOTP(IP) +C AUTHOR RODGER STADEN + CHARACTER PUP*26 + SAVE PUP + DATA PUP/'CSTPAGNDEQBZHRKMILVFYW-X? '/ + MUNOTP = '-' + IF((IP.GT.0).AND.(IP.LT.23))MUNOTP = PUP(IP:IP) + END + INTEGER FUNCTION NCDEP(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,IGEL, + +STRAND,RREG) + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG,STRAND + NCDEP = 0 + N = 0 + I = IGEL +10 CONTINUE + IF(I.NE.0) THEN + IF(RELPG(I).LE.RREG) THEN + IF(SIGN(1,LNGTHG(I)).EQ.STRAND) N = N + 1 + I = RNBR(I) + GO TO 10 + END IF + END IF + NCDEP = N + END + SUBROUTINE NEWDB(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,KBOUT, + +IDEVF,IDEVR,IDEVW,IDEVN,MAXDB,WGEL,MAXGEL,LINCON,KGEL,IOK) +C AUTHOR: RODGER STADEN + INTEGER RELPG(MAXDB),LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER LINEIN*80,GEL*62,WGEL(MAXGEL),GELNAM*10 + EQUIVALENCE (LINEIN(18:18),GEL) + EQUIVALENCE (LINEIN(8:8),GELNAM) + EXTERNAL LTYPE + LINLEN = 80 + LINENO=0 +C SET LENGTH OF CONTIG + LENCON=0 +C SET POSITION OF RIGHTMOST CHAR OF LAST BLOCK + ILASTR=0 +C SET NUMBER OF LAST GEL TO START SO WE CAN IDENTIFY RIGHT AND LEFT +C NEIGHBOURS + LASTS=0 +C NEED TO WHERE THE LEFT OF EACH BLOCK IS. ASSUME THE FIRST GEL IN A BLOCK +C IS LEVEL WITH THE LEFT EDGE FOR THAT BLOCK (IT SHOULD BE). SO NEED A FLAG +C (IGF)=0 IF FIRST =1 IF NOT, AND A POSITION (ILEFT) TO MARK THE LEFT EDGE +C NEED TO SET LEFT EDGE TO 1 SO LENGTH CALCULATION WORKS FOR FIRST BLOCK + ILEFT=1 +10 CONTINUE +C NEED TO ADD LENGTH OF LONGEST LINE IN LAST BLOCK TO CONSENSUS LENGTH + LENCON=LENCON+ILASTR-ILEFT+1 +C RESET POSITION OF RIGHTMOST CHAR OF LAST BLOCK + ILASTR=0 +C READ LINE OF NOS + READ(IDEVF,1003,END=100)LINEIN +1003 FORMAT(A) + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) +C WRITE(*,*)LINENO,LT + IF(LT.NE.2) GO TO 400 +C SET FLAG FOR NEW BLOCK + IGF=0 +20 CONTINUE +C +C READ A LINE, COULD BE A GEL OR A CONSENSUS + READ(IDEVF,1003,END=100)LINEIN + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) +C WRITE(*,*)LINENO,LT + IF(LT.EQ.0) GO TO 400 +C WHAT SORT OF LINE? + IF(LT.EQ.1) GO TO 400 + IF(LT.EQ.4) THEN + IFIRST = J1 - 17 + ILAST = J2 - 17 + NPOS=ABS(N) +C GEL LINE SO SAVE +C NEED TO FIND THE RIGHTMOST POSITION FOR EACH BLOCK + IF(ILAST.GT.ILASTR)ILASTR=ILAST +C IF FIRST GEL THIS BLOCK SAVE POSITION OF LEFT EDGE + IF(IGF.EQ.0)ILEFT=IFIRST +C SET FLAG FOR NOT FIRST GEL THIS BLOCK + IGF=1 +C START OF GEL? + IF(LNGTHG(NPOS).EQ.0)THEN +C YES SO SET RELPG + RELPG(NPOS)=LENCON+IFIRST-ILEFT+1 +C LNBR(NPOS)=LASTS +C SET RIGHT NEIGHBOUR OF LAST GEL TO START +C IF(LASTS.NE.0)RNBR(LASTS)=NPOS + LASTS=NPOS + CALL WRITEN(IDEVN,NPOS,GELNAM) + END IF +C COPY NEW SEQUENCE TO DISK + CALL READW(IDEVW,NPOS,WGEL,MAXGEL) + K1=ABS(LNGTHG(NPOS))+1 + K2=K1+ILAST-IFIRST + K11=IFIRST-1 +C WRITE(*,*)K1,K2,K11 + DO 25 I=K1,K2 + K11=K11+1 + WGEL(I)=GEL(K11:K11) +25 CONTINUE + CALL WRITEW(IDEVW,NPOS,WGEL,MAXGEL) +C UPDATE LENGTH + LNGTHG(NPOS)=SIGN(K2,N) +C GO BACK FOR NEXT LINE (CONSENSUS OR NEW GEL) + GO TO 20 + END IF +C SHOULD BE CONSENSUS, CHECK + IF(LT.NE.3) GO TO 400 +C READ WHAT SHOULD BE A BLANK LINE + READ(IDEVF,1003,END=100)LINEIN + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,IFIRST,ILAST,N,MAXDB,KBOUT) +C WRITE(*,*)LINENO,LT + IF(LT.NE.1) GO TO 400 + GO TO 10 +100 CONTINUE +C CHECK FOR MISSING BLANK AT END OF FILE + IF(LT.NE.1) LENCON=LENCON+ILASTR-ILEFT+1 + LNBR(LINCON) = KGEL + CALL MERGE(RELPG,LNGTHG,LNBR,RNBR,LINCON,IDBSIZ) +C ON INPUT LNBR(LINCON) MAY BE WRONG, BUT ON EXIT RNBR IS CORRECT + I = RNBR(LINCON) +300 CONTINUE +C WRITE(*,*)I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + J = I + I = LNBR(I) + IF(I.NE.0) GO TO 300 +C WRITE CONTIG LINE + I = LINCON + RELPG(I) = LENCON + LNGTHG(I) = 0 + LNBR(I) = J +C WRITE(*,*)I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + WRITE(KBOUT,1005) +1005 FORMAT(' Finished, no errors found') +C + IOK = 0 + RETURN +400 CONTINUE +C ERROR DETECTED, REPORT LINE NUMBER AND STOP + WRITE(KBOUT,5002)LINENO +5002 FORMAT(' Error in line ',I6,' of file. Processing stopped', + +/,' but your database may be corrupted. Revert to a copy') + IOK = 1 + RETURN + END + SUBROUTINE PADCOP(SEQG,SEQG2,LG1,MG,L5,IS2,LG2,MAXGEL,IFAIL, + +KBOUT,SEQC,IC1) +C AUTHOR: RODGER STADEN + PARAMETER (NDUBL = 4) + CHARACTER SEQG(MAXGEL),SEQG2(MAXGEL),DUBBL(NDUBL),SEQC(MAXGEL) + SAVE DUBBL + DATA DUBBL/'D','B','V','H'/ + JC1 = IC1 +C Make seqg2 from seqg placing L5 padding chars before position MG +C which is the start of the next block of identity. Try to put the +C padding either in line with consensus pads, or next to double +C codes. The positions in seqg are LG1 to MG-1. seqg2 needs to be long +C enough to be extended from IS2 to IS2 + L5 -1 + MGM1-LG1 +1 +C ie we add L5 pads, plus the chars between and including LG1 and MGM1 + IDONE=0 +C POINT TO END OF MISMATCH + MGM1=MG-1 +C MAY BE NO CHARS TO COPY + IF(MGM1.LT.LG1)GO TO 111 +C Next check added 26-2-91 + MAXREQ = IS2 + L5 - 1 + MGM1 - LG1 + 1 + IF((MGM1.GT.MAXGEL).OR.(MAXREQ.GT.MAXGEL)) THEN + WRITE(KBOUT,1000) +1000 FORMAT(' Matching region too large for routine padcop,', + + ' alignment aborted') + IFAIL=1 + RETURN + END IF + DO 110 J=LG1,MGM1 + IF(IDONE.LT.L5) THEN + IF((JC1.GT.0).AND.(JC1.LT.MAXGEL)) THEN + IF(SEQC(JC1).EQ.'*') THEN + IS2 = IS2 + 1 + JC1 = JC1 + 1 + IDONE = IDONE + 1 + GO TO 109 + END IF + END IF + DO 108 M=1,NDUBL + IF(SEQG(J).EQ.DUBBL(M)) THEN + IS2 = IS2 + 1 + JC1 = JC1 + 1 + IDONE = IDONE + 1 + GO TO 109 + END IF +108 CONTINUE +109 CONTINUE + END IF + SEQG2(IS2) = SEQG(J) + IS2 = IS2 + 1 + JC1 = JC1 + 1 +110 CONTINUE +111 CONTINUE +C ALL CHARS COPIED. ENOUGH PADDING? + IF(IDONE.LT.L5)IS2=IS2+L5-IDONE +C IS2 SHOULD NOW BE POINTING AT NEXT CHAR +C ZERO LG2 TO SHOW CALLING ROUTINE COPYING DONE + LG2=0 + IFAIL=0 + END + SUBROUTINE PADRUN(GEL,IFIRST,ILAST,RUN) + CHARACTER PAD,GEL*50,RUN*50 + SAVE PAD + DATA PAD/'*'/ +C FIND PADS IN THIS GEL + DO 20 I = IFIRST,ILAST + IF(GEL(I:I).EQ.PAD) THEN +C IS IT AT LEAST THE SECOND IN A RUN + IF(RUN(I:I).EQ.PAD) THEN +C FIND THE LEFT END OF THE RUN + JJ = I +1 CONTINUE + IF(JJ.GT.1)THEN + JJ = JJ - 1 + IF(RUN(JJ:JJ).EQ.PAD)GO TO 1 + END IF +C FOUND LEFT END OF RUN AT JJ, SO MOVE PAD IF NOT ALREADY A PAD +5 CONTINUE + IF(GEL(JJ:JJ).NE.PAD)THEN +C EXCHANGE ELEMENTS + GEL(I:I) = GEL(JJ:JJ) + GEL(JJ:JJ) = PAD + ELSE +C ALREADY A PAD, SO TRY NEXT TO RIGHT + JJ = JJ + 1 + IF(JJ.LT.I) GO TO 5 + END IF + END IF + END IF +20 CONTINUE + END + SUBROUTINE PCON1(CHAR,CHRSUM) +C AUTHOR RODGER STADEN +C PART OF PROTEIN 'CONSENSUS' CALCULATION + CHARACTER CHAR + INTEGER CHRSUM + INTEGER CTONUM + EXTERNAL CTONUM + K = CTONUM(CHAR) + IF(K.NE.26)THEN + IF(CHRSUM.EQ.0)THEN + CHRSUM = K + ELSE + IF(K.NE.CHRSUM)CHRSUM = -1 + END IF + END IF + END + SUBROUTINE PLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LINCON,IGEL, + +NCONTS,MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + YMIN = 0. + YMAX = ISYMAX + XMIN = 0. + LENCON = 0 + DO 10 I = IDBSIZ-NCONTS,IDBSIZ-1 + LENCON = LENCON + RELPG(I) +10 CONTINUE + XMAX = LENCON + YINC = (YMAX-YMIN)/3. + Y = 0. + XF = XMIN + N = 0 + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + DO 20 I = IDBSIZ-NCONTS,IDBSIZ-1 + N = N + 1 + XT = XF + RELPG(I) + Y = Y + YINC + CALL LINE(XF,XT,Y,Y,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + IF((IGEL.NE.0).AND.(I.EQ.LINCON)) THEN + XZ = XF + RELPG(IGEL) + ABS(LNGTHG(IGEL))/2 + CALL LINE(XZ,XZ,YMAX,YMIN,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + XF = XT + IF(N.EQ.2) THEN + N = 0 + Y = 0. + END IF +20 CONTINUE + CALL VT100M + END + SUBROUTINE PLTC(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,STRAND, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,YMID,YINC,DEPTH) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG,STRAND,DEPTH + XMIN = LREG + XMAX = RREG + YMAX = ISYMAX + YMIN = 0. + I = LGEL +5 CONTINUE + IF(I.NE.0) THEN + IF((RELPG(I)+ABS(LNGTHG(I))-1).LT.LREG) THEN + I = RNBR(I) + GO TO 5 + END IF + END IF + N = 0 +10 CONTINUE + IF(I.NE.0)THEN + IF(RELPG(I).LE.RREG) THEN + IF(SIGN(1,LNGTHG(I)).EQ.STRAND) THEN + XF = MAX(RELPG(I),LREG) + XT = MIN(ABS(LNGTHG(I))+RELPG(I)-1,RREG) + N = N + 1 + IF(N.GT.DEPTH) N = 1 + YF = YMID + N * YINC + CALL LINE(XF,XT,YF,YF,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + I = RNBR(I) + GO TO 10 + END IF + END IF + END + SUBROUTINE PLTCON(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ, + +MARGL,MARGR,MARGB, + +MARGT,ISXMAX,ISYMAX,LGEL,LREG,RREG,DEPTHP,DEPTHM) + INTEGER DEPTHP,DEPTHM + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG,STRAND +C have window size margt starting at margb +C depths depthp, depthm + YMAX = ISYMAX + YMIN = 0. + XMIN = LREG + XMAX = RREG + RINC = YMAX / (DEPTHP + DEPTHM + 2) + RMID =(DEPTHM+1) * RINC + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(XMIN,XMAX,RMID,RMID,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL TEXT(XMIN,RMID,'*',1,ISIZE,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL TEXT(XMAX,RMID,'*',1,ISIZE,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + STRAND = 1 + YINC = RINC * STRAND + CALL PLTC(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,STRAND, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,RMID,YINC,DEPTHP) + STRAND = -1 + YINC = RINC * STRAND + CALL PLTC(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,STRAND, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,RMID,YINC,DEPTHM) + CALL VT100M + END + SUBROUTINE PLTQ(SEQ,IDIM2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CHARACTER SEQ(IDIM2),T + PARAMETER (Y0 = 0., + + YP1 = 1., + + YP2 = 2., + + YM1 = -1., + + YM2 = -2.) + XMIN = 0. + XMAX = IDIM2 + YMIN = YM2 + YMAX = YP2 + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(XIN,XMAX,Y0,Y0,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + I = 1 +10 CONTINUE + XF = I + T = SEQ(I) +20 CONTINUE + IF(SEQ(I).NE.T) THEN + CALL GLEVEL(T,YF,YT,Y0,YP1,YP2,YM1,YM2) + XT = I - 1 + CALL LINE(XF,XF,YF,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(XF,XT,YT,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(XT,XT,YF,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + GO TO 10 + END IF + I = I + 1 + IF(I.LT.IDIM2) GO TO 20 + CALL GLEVEL(T,YF,YT,Y0,YP1,YP2,YM1,YM2) + XT = I + CALL LINE(XF,XF,YF,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(XF,XT,YT,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(XT,XT,YF,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL VT100M + END + SUBROUTINE POSTC(GELNOS,GELSTR,GELEND,LINEIN,IERR, + +KBOUT,IDEVF,MAXDB,GELNO,LINNO,MAXLIN,RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,KGEL) +C AUTHOR: RODGER STADEN + CHARACTER LINEIN*(*) + INTEGER GELNOS(MAXDB),GELSTR(MAXDB),GELEND(MAXDB) + INTEGER GELNO(MAXLIN,2),LINNO(MAXLIN,2),IGEL(2) + INTEGER LNBR(MAXDB),RNBR(MAXDB),RELPG(MAXDB),LNGTHG(MAXDB) + EXTERNAL LTYPE +C GELNOS === GEL NUMBERS (UNIQUE) +C GELSTR === GEL START LINES +C GELEND === GEL END LINES +C LINNO === GEL LINE NUMBERS PER STRIP +C IGEL === NUMBER OF GELS PER STRIP +C LINENO === CURRENT LINE NUMBER +C IUNIQ IS NUMBER OF GELS FOUND +C +C PROBLEM IS TO FIND IF THERE ARE LINES OF WORKING VERSIONS MISSING +C WE PROCESS THE DATA IN PAIRS OF BLOCKS OR STRIPS (A BLOCK OR STRIP IS ONE 50 +C CHARACTER WIDTH OF THE CONTIG). IF WE FIND ALL THE GELS THAT APPEAR +C IN STRIP B THEN IF THEY WEREN'T PRESENT IN STRIP A THEY MUST START IN +C STRIP B, IF THEY DONT APPEAR IN STRIP C THEY END IN STRIP B. +C STRATEGY IS TO READ THRU FILE AND REMEMBER WHICH GELS APPEAR IN EACH +C STRIP. BY COMPARING THEM WITH THE LAST STRIP WE CAN KNOW WHICH HAVE ENDED +C AND WHICH HAVE STARTED. HENCE WE STORE A LIST OF GELS AND THEIR START AND +C END LINE NUMBERS. USING THIS WE CAN SEE IF THE LIST IS UNIQUE +C SUB CLIST IS SENT THE CURRENT TWO LISTS OF GEL NUMBERS AND FINDS WHICH HAVE +C STARTED AND ENDED AND BUILDS UP A LIST OF UNIQUE GELS AND THEIR START +C AND END LINE NUMBERS +C + LINLEN = 80 + IERR=0 + JSTRIP=1 + LINENO=0 + ISTRIP=1 + IUNIQ=0 +10 CONTINUE +C READ WHAT SHOULD BE A LINE OF NOS + READ(IDEVF,1003,END=100)LINEIN +1003 FORMAT(A) + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) +C IS IT A LINE OF NOS? + IF(LT.NE.2) GO TO 400 +C ZERO GEL COUNT FOR THIS STRIP + IGEL(ISTRIP)=0 +20 CONTINUE +C READ A LINE, COULD BE 1 GEL, 2 CONSENSUS OR BLANK + READ(IDEVF,1003,END=100)LINEIN + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) + IF(LT.EQ.0) GO TO 400 + IF(LT.EQ.1) GO TO 400 + IF(LT.EQ.4) THEN +C GEL LINE SO SAVE +C INCREMENT COUNT TO NUMBER OF GEL + IGEL(ISTRIP)=IGEL(ISTRIP)+1 + GELNO(IGEL(ISTRIP),ISTRIP)=N + LINNO(IGEL(ISTRIP),ISTRIP)=LINENO +C BO BACK FOR NEXT LINE (CONSENSUS OR NEW GEL) + GO TO 20 + END IF +C SHOULD THEN BE A CONSENSUS + IF(LT.NE.3) GO TO 400 +C MUST BE CONSENSUS SO PROCESS THIS STRIP +C PROCESS THIS STRIP OF GELS (IGEL OF THEM) +C WHICH GELS ARE NEW? AND WHICH HAVE ENDED +C IF FIRST EVER STRIP DONT DO THIS + IF(JSTRIP.EQ.1)THEN + DO 6 I=1,IGEL(1) + GELNOS(I)=GELNO(I,1) + GELSTR(I)=LINNO(I,1) +6 CONTINUE + JSTRIP=JSTRIP+1 + ISTRIP=2 + IUNIQ=IGEL(1) +C READ WHAT SHOULD BE A BLANK LINE + READ(IDEVF,1003,END=100)LINEIN + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) + IF(LT.EQ.0) GO TO 400 + IF(LT.NE.1)GO TO 400 + GO TO 10 + END IF +C ORER OF ARGUMENTS DEPENDS ON ISTRIP + IF(ISTRIP.EQ.2)THEN + CALL CLIST(GELNO(1,1),LINNO(1,1),IGEL(1), + + GELNO(1,2),LINNO(1,2),IGEL(2), + + GELNOS,GELSTR,GELEND,MAXDB,IUNIQ,KBOUT,IERR) + IF(IERR.NE.0) GO TO 400 + ISTRIP=1 +C READ WHAT SHOULD BE A BLANK LINE + READ(IDEVF,1003,END=100)LINEIN + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) + IF(LT.EQ.0) GO TO 400 + IF(LT.NE.1)GO TO 400 + GO TO 10 + END IF + IF(ISTRIP.EQ.1)THEN + CALL CLIST(GELNO(1,2),LINNO(1,2),IGEL(2), + + GELNO(1,1),LINNO(1,1),IGEL(1), + + GELNOS,GELSTR,GELEND,MAXDB,IUNIQ,KBOUT,IERR) + IF(IERR.NE.0) GO TO 400 + ISTRIP=2 + READ(IDEVF,1003,END=100)LINEIN + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) + IF(LT.EQ.0) GO TO 400 + IF(LT.NE.1)GO TO 400 + GO TO 10 + END IF +100 CONTINUE +C MAY BE SOME LEFT IN CURRENT STRIP THAT HAVE NOT BEEN ENDED +C BUT ISTRIP WILL JUST HAVE BEEN CHANGED SO CHANGE IT BACK + IF(ISTRIP.EQ.1)THEN + ISTRIP=2 + GO TO 101 + END IF + ISTRIP=1 +101 CONTINUE + DO 110 I=1,IGEL(ISTRIP) + MATCH=INLIST(GELNOS,IUNIQ,GELNO(I,ISTRIP)) + IF(MATCH.NE.0)THEN + GELEND(MATCH)=LINNO(I,ISTRIP) + GO TO 110 + END IF +C ERROR + WRITE(KBOUT,1300)GELNO(I,ISTRIP) +1300 FORMAT( ' Error: gel number ',I5, + + ' expected but not found in list') + IERR = 1 + GO TO 400 +110 CONTINUE +C AS A CHECK: ARE THE UNIQUE GELS UNIQUE? + DO 120 I=2,IUNIQ + KD=IUNIQ-I+1 + IN=INLIST(GELNOS(I),KD,GELNOS(I-1)) + IF(IN.EQ.0)GO TO 120 + IERR=1 + WRITE(KBOUT,1006)GELNOS(I-1),GELSTR(I-1),GELSTR(IN) +1006 FORMAT(' Error: gel',I6,' has a line of data missing!'/ + + ' and appears to start on lines',I6,' and',I6) + GO TO 400 +120 CONTINUE + IF(IERR.EQ.0)WRITE(KBOUT,1005) +1005 FORMAT(' Successfully checked line order and missing lines') +C WRITE(*,*)(GELNOS(K),K=1,IUNIQ) + KGEL1 = KGEL + KGEL = ABS(GELNOS(1)) +C CHECK THAT THE LEFT GEL NUMBER IS UNCHANGED + IF(KGEL1.NE.KGEL) THEN + WRITE(KBOUT,1001) +1001 FORMAT(' Left gel number of edited contig does not agree',/, + + ' with original, processing aborted') + IERR = 1 + RETURN + END IF + LNBR(KGEL) = 0 + RELPG(KGEL) = 0 + LNGTHG(KGEL) = 0 + DO 200 I = 2,IUNIQ + J1 = ABS(GELNOS(I-1)) + J2 = ABS(GELNOS(I)) + RNBR(J1) = J2 + LNBR(J2) = J1 + RELPG(J2) = 0 + LNGTHG(J2) = 0 +200 CONTINUE + RNBR(J2) = 0 + IERR = 0 + RETURN +400 CONTINUE +C ERROR + WRITE(KBOUT,1007)LINENO +1007 FORMAT(' Error in line',I6,', of file: lines out of order'/, + +' processing stopped') + IERR=1 + RETURN + END + SUBROUTINE READN(IDEVN,N,NAME) + CHARACTER NAME*(*) + READ(IDEVN,REC=N)NAME + RETURN + END + SUBROUTINE READR(IDEVR,N,RELPG,LNGTHG,LNBR,RNBR) + INTEGER RELPG,RNBR + READ(IDEVR,REC=N)RELPG,LNGTHG,LNBR,RNBR + RETURN + END + SUBROUTINE READW(IDEVW,N,GEL,MAXGEL) + CHARACTER GEL(MAXGEL) + READ(IDEVW,REC=N)GEL + RETURN + END + SUBROUTINE REMOVL(MATC,MATG,MATL,IP) +C AUTHOR: RODGER STADEN + INTEGER MATC(IP),MATG(IP),MATL(IP) +C +C SET POINTER TO FIRST MATCH + NMTCH=0 +10 CONTINUE +C POINT TO NEXT MATCH + NMTCH=NMTCH+1 +C SORT MATCHES ON LENGTH + IPP=IP-NMTCH+1 + CALL BUBBL3(MATL(NMTCH),MATG(NMTCH),MATC(NMTCH),IPP) +C LOOK FOR END OF POSITIVES + DO 20 I=NMTCH,IP + J=I +20 IF(MATL(I).LT.1)GO TO 30 + J=J+1 +30 CONTINUE + IP=J-1 +C END OF POSITIVES AT IP + IF(NMTCH.GE.IP)RETURN + K1=MATC(NMTCH) + K2=K1+MATL(NMTCH)-1 + K3=MATG(NMTCH) + K4=K3+MATL(NMTCH)-1 +C POINT TO FIRST MATCH TO TEST + K6=NMTCH+1 + DO 200 I=K6,IP +C DO CONSENSUS FIRST +C OVERLAP? + IF(MATC(I).GT.K2)GO TO 100 + K5=MATC(I)+MATL(I)-1 + IF(K5.LT.K1)GO TO 100 +C DOES OVERLAP +C WHICH END + IF(K5.LE.K2)GO TO 80 +C LENGTH TO REDUCE MATCH BY IS IDELT + IDELT=K2-MATC(I)+1 +C NEW LENGTH + MATL(I)=MATL(I)-IDELT +C MOVE LEFT ENDS + MATC(I)=MATC(I)+IDELT + MATG(I)=MATG(I)+IDELT + GO TO 100 +80 CONTINUE +C LENGTH + MATL(I)=K1-MATC(I) +100 CONTINUE +C NOW LOOK FOR OVERLAPS WITH GEL +C OVERLAP? + IF(MATG(I).GT.K4)GO TO 200 + K5=MATG(I)+MATL(I)-1 + IF(K5.LT.K3)GO TO 200 +C DOES OVERLAP +C WHICH END? + IF(K5.LE.K4)GO TO 180 +C LENGTH TO REDUCE MATCH BY IS IDELT + IDELT=K4-MATG(I)+1 +C NEW LENGTH + MATL(I)=MATL(I)-IDELT +C MOVE LEFT ENDS + MATC(I)=MATC(I)+IDELT + MATG(I)=MATG(I)+IDELT + GO TO 200 +180 CONTINUE +C LENGTH + MATL(I)=K3-MATG(I) +200 CONTINUE + GO TO 10 + END +C SAVIT +C + SUBROUTINE SAVIT(N,J,K,IP,S1,S2,S3,IP1) +C AUTHOR: RODGER STADEN + INTEGER S1(IP1),S2(IP1),S3(IP1) +C + IP=IP+1 +C TEST FOR OVERFLOW + IF(IP.GT.IP1)RETURN + S1(IP)=N + S2(IP)=J-N + S3(IP)=K-N +C + RETURN + END + SUBROUTINE SCRENR(GEL,MAXGEL,STRING,NAME,FILNAM, + +IDEV1,IDEV2,IDEV3,IDEV4,IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER NAME*(*),FILNAM*(*),HELPF*(*) + CHARACTER GEL(MAXGEL),STRING(60) + CALL YESNO(INF,'Use file of file names', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(INF.LT.0) RETURN + IF(INF.EQ.0) THEN + FILNAM = ' ' + CALL OPENF1(IDEV1,FILNAM,0,IOK,KBIN,KBOUT, + + 'File of gel reading names', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,1,IOK,KBIN,KBOUT, + + 'File for names of sequences that pass', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + END IF + FILNAM = ' ' + CALL OPENF1(IDEV3,FILNAM,0,IOK,KBIN,KBOUT, + +'File name of recognition sequences', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN +C + JGEL = 0 + IGEL = 0 +1 CONTINUE + IF(INF.EQ.1) THEN +31 CONTINUE + MN = 0 + CALL GTSTR('Gel reading name',' ',NAME,MN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.3) RETURN + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 31 + END IF + ELSE + READ(IDEV1,1002,END=100)NAME + END IF +1002 FORMAT(A) + JGEL = JGEL + 1 + WRITE(IDEV,*)'Processing', JGEL,' in batch' + WRITE(IDEV,1003)NAME +1003 FORMAT(' Gel reading name ',A) + IDIMG=MAXGEL + CALL OPENRS(IDEV4,NAME,IOK,LRECL,2) + IF(IOK.NE.0)THEN + IF(INF.EQ.1) RETURN + WRITE(KBOUT,*)' Error opening gel reading file' + GO TO 1 + END IF + CALL ARRFIM(IDEV4,GEL,IDIMG,KBOUT) + CLOSE(UNIT=IDEV4) +2 CONTINUE + IF(IDIMG.LT.1)THEN + WRITE(KBOUT,*)' Gel reading too short to compare' + GO TO 1 + END IF + CALL MSTLKL(GEL,IDIMG) +3 CONTINUE + READ(IDEV3,1005,END=6)STRING +1005 FORMAT(60A1) +C FIND LENGTH OF STRING ASSUMING NO SPACES + DO 4 I=1,60 + II=I + IF(STRING(I).EQ.' ')GO TO 5 +4 CONTINUE +5 CONTINUE + II=II-1 + IF(II.GT.0)CALL FIND(GEL,IDIMG,STRING,II,JMATCH) + IF(JMATCH.EQ.0)GO TO 3 +C A MATCH + WRITE(IDEV,1007)JMATCH,(STRING(K),K=1,II) +1007 FORMAT(' Match at',I6,' with ',60A1) + REWIND IDEV3 + GO TO 1 +C NO MATCH SO SAVE +6 CONTINUE + WRITE(IDEV2,1002)NAME + IGEL = IGEL + 1 + REWIND IDEV3 + GO TO 1 +100 CONTINUE + WRITE(KBOUT,*)'Batch finished' + WRITE(KBOUT,*)JGEL,' compared and ',IGEL,' passed' + RETURN + END + SUBROUTINE SCRENV(MAXGEL, + +WORDP,WORDN,LPOWRC,POSNS,GELN, + +SEQ,MAXSEQ,GEL,GELCOP,MATCH, + +LENGTH, + +SAVPS,SAVPG,SAVL,MAXMAT,CENDS,NENDS,MAXCON,CONST, + +KBIN,KBOUT,IDEV1,IDEV2,IDEV3,IDEV4,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,FILNAM,NAME,IOK) + INTEGER POSNS(MAXSEQ),GELN(MAXGEL),WORDP(LPOWRC),SAVPS(MAXMAT) + INTEGER SAVPG(MAXMAT),SAVL(MAXMAT) + INTEGER WORDN(LPOWRC) + CHARACTER FILNAM*(*),NAME*(*),HELPF*(*) + CHARACTER GELCOP(MAXGEL) + INTEGER CENDS(MAXCON) + INTEGER NENDS(MAXCON) + INTEGER CONST(LENGTH) + CHARACTER SEQ(MAXSEQ),GEL(MAXGEL),MATCH(MAXGEL) + JGEL = 0 + IGELS = 0 + CALL YESNO(INF,'Use file of file names', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(INF.LT.0) RETURN + IF(INF.EQ.0) THEN + FILNAM = ' ' + CALL OPENF1(IDEV1,FILNAM,0,IOK,KBIN,KBOUT, + + 'File of gel reading names', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,1,IOK,KBIN,KBOUT, + + 'File for names of gel readings that pass', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + END IF + FILNAM = ' ' + CALL OPENF1(IDEV4,FILNAM,0,IOK,KBIN,KBOUT, + +'File name of vector sequence', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + IDIM = MAXSEQ + CALL ARRFIM(IDEV4,SEQ,IDIM,KBOUT) + CLOSE(UNIT=IDEV4) + MN = LENGTH*2 + MX = 50 + MINMAT = MAX(15,MN) + CALL GETINT(MN,MX,MINMAT, + +'Minimum initial match', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINMAT = IVAL + IDCEND=MAXCON + CALL FNDCON(SEQ,IDIM,CENDS,NENDS,IDCEND,MAXCON,KBOUT) +C IS THE VECTOR SEQUENCE IN THE CORRECT FORMAT WITH A TITLE AT THE FRONT? + IF(IDCEND.EQ.0)THEN + CENDS(1) = -19 + NENDS(1) = 1 + CENDS(2) = IDIM + 1 + IDCEND = 1 + END IF +C WRITE(KBOUT,9999) +C9999 FORMAT(' VECTOR SEQUENCE REQUIRES A TITLE EG ', +C 1' <---M13MP7.001----->') +C RETURN +C END IF + CALL BUSY(KBOUT) + CALL ENCO(SEQ,IDIM,POSNS,CONST,LENGTH) + CALL ENCONA(POSNS,IDIM,WORDP,WORDN,LPOWRC,LENGTH) +C +1 CONTINUE + IF(INF.EQ.1) THEN +3 CONTINUE + MN = 0 + CALL GTSTR('Gel reading name',' ',NAME,MN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.3) RETURN + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 3 + END IF + ELSE + READ(IDEV1,1002,END=100)NAME + END IF + JGEL = JGEL + 1 + WRITE(IDEV,*)'Processing',JGEL,' in batch' +1002 FORMAT(A) + WRITE(IDEV,1003)NAME +1003 FORMAT(' Gel reading name ',A) + IDIMG=MAXGEL + CALL OPENRS(IDEV3,NAME,IOK,LRECL,2) + IF(IOK.NE.0)THEN + IF(INF.EQ.1) RETURN + WRITE(IDEV,*)' Gel reading file not found' + GO TO 1 + END IF + CALL ARRFIM(IDEV3,GEL,IDIMG,KBOUT) + CLOSE(UNIT=IDEV3) +C LONG ENOUGH ? + IF(IDIMG.LT.MINMAT)THEN + WRITE(IDEV,*)' Gel reading too short to compare' + GO TO 1 + END IF + CALL SQCOPY(GEL,GELCOP,IDIMG) + ISTRAN=1 + IMATCH=0 +2 CONTINUE + CALL BUSY(KBOUT) + CALL MSTLKL(GEL,IDIMG) + CALL ENCO(GEL,IDIMG,GELN,CONST,LENGTH) + WRITE(IDEV,1009)ISTRAN +1009 FORMAT(' Searching strand',I6) + IDSAV=MAXMAT + CALL CFGEL(GELN,IDIMG,POSNS,IDIM,WORDP,WORDN,LENGTH,LPOWRC, + +SAVPG,SAVPS,SAVL, + +IDSAV,SEQ,GELCOP,MINMAT,IFAIL,KBOUT) + IF(IDSAV.GT.0) THEN + IMATCH=1 + CALL DISMAT(SEQ,IDIM,GELCOP,IDIMG,SAVPS,SAVPG,IDSAV, + + CENDS,NENDS,IDCEND,MAXCON,IDEV,MATCH) + END IF + IF(ISTRAN.EQ.1) THEN + CALL SQREV(GELCOP,IDIMG) + CALL SQCOM(GELCOP,IDIMG) + CALL SQCOPY(GELCOP,GEL,IDIMG) + ISTRAN = 2 + GO TO 2 + END IF + IF(IMATCH.EQ.0) THEN + WRITE(IDEV2,1010)NAME + IGELS = IGELS + 1 + END IF + GO TO 1 +1010 FORMAT(A) +100 CONTINUE + WRITE(KBOUT,*)'Batch finished' + WRITE(KBOUT,*)JGEL,' compared and ',IGELS,' passed' + RETURN + END + SUBROUTINE SHIFTC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDEVR, + +IDBSIZ,IGN,NCONT,DIST) +C AUTHOR: RODGER STADEN +C SHIFTS PART OF A CONTIG FORM GEL IGN TO RIGHT END +C CONTIG LINE NUMBER IF NCONT + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER DIST + I = IGN +10 CONTINUE + IF(I.NE.0)THEN + RELPG(I) = RELPG(I) + DIST + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + I = RNBR(I) + GO TO 10 + END IF +C UPDATE CONTIG LENGTH + RELPG(NCONT) = RELPG(NCONT) + DIST + CALL WRITER(IDEVR,NCONT,RELPG(NCONT),LNGTHG(NCONT), + +LNBR(NCONT),RNBR(NCONT)) + END + SUBROUTINE SLIDER(SEQ1,IDC,SEQ2,IDIM2,MS1,MS2,MAXPG,MAXPC,MINSLI, + +MATL,MATC,MATG,IP) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDC),SEQ2(IDIM2) + INTEGER MATL(IP),MATC(IP),MATG(IP),P1S,P1,P2 + IP1 = IP + IP = 0 +C LEFT END S2 RELATIVE S1 - MAX PADS -2 READY FOR LOOP + P1S = MS1 - MS2 - MAXPC - 1 +C TRY NSLIDE START POSNS FOR SEQ2 + DO 100 I=1,MAXPG+MAXPC+1 +C POINT TO SEQ1 START + P1S = P1S + 1 +C POINT TO CURRENT SEQ1 POSN + P1 = P1S + N = 0 +C COMPARE WHOLE LENGTH OF SEQ2 (IF P1 WITHIN RANGE) + DO 50 J=1,IDIM2 + P2 = J + P1 = P1 + 1 + IF(P1.LT.1)GO TO 50 +C OFF RIGHT END? IF SO MAY HAVE BEEN A MATCH + IF(P1.GT.IDC)GO TO 40 + IF(SEQ1(P1).EQ.SEQ2(P2))GO TO 45 +40 CONTINUE + IF(N.GE.MINSLI)CALL SAVIT(N,P1,P2,IP,MATL,MATC,MATG,IP1) + N = 0 + GO TO 50 +45 CONTINUE + N = N + 1 +50 CONTINUE +C GOOD SCORE AT END? NEED TO INCREMENT POINTERS FOR SAVIT + P1 = P1 + 1 + P2 = P2 + 1 + IF(N.GE.MINSLI)CALL SAVIT(N,P1,P2,IP,MATL,MATC,MATG,IP1) +100 CONTINUE + END + SUBROUTINE SUBS(SEQ,IDIMS,FROM,TO) + CHARACTER SEQ(IDIMS),FROM,TO +C AUTHOR RODGER STADEN + DO 10 I = 1,IDIMS + IF(SEQ(I).EQ.FROM) SEQ(I) = TO +10 CONTINUE + END + SUBROUTINE SUMMAR(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,LREG,RREG,IGELC,PERCD,IDBSIZ,CHARS, + +ID1,CHRSIZ,MAXGL2,IDEVW,MAXGEL,LINOU1,LINOU2,MXGOOD) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),CHRSIZ + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER SEQ1(IDIM1) + CHARACTER GEL(MAXGEL) + INTEGER LREG,RREG,LSEQNO,POSN,Y,POSN1 + INTEGER GELC + CHARACTER LINOU1(MAXGEL),LINOU2(MAXGEL),GTCONC + INTEGER CHARS(CHRSIZ,ID1,MAXGL2) + EXTERNAL INDEXS,LWRAPS,GTCONC +C 28-7-91 added extra parameter: mxgood is the maximum reading +C length for which we have confidence, so only the parts of +C reads 1 to mxgood will be included in the calculation +C SET INITIAL VALUES +C hard to understand this very old code! rewrite it. +C we have a summing array of twice the length of the longest sequence +C posn is posn in contig of next to write, lseqno is current posn in contig +C we write when lseqno-posn ge the length of the longest seq + POSN=LREG + GELC=IGELC + LINLEN=MAXGEL + LSEQNO=RELPG(GELC) + IEND=0 + DO 40 I=1,MAXGL2 + DO 40 J=1,ID1 + DO 40 K=1,CHRSIZ + CHARS(K,J,I)=0 +40 CONTINUE +50 CONTINUE + ISS=1 + IF(LNGTHG(GELC).LT.0)ISS=2 + CALL READW(IDEVW,GELC,GEL,MAXGEL) +C LOOP FOR RELEVANT ELEMENTS THIS GEL +C only use mxgood characters from start of read +C + IF(ISS.EQ.1) THEN + N = MIN(MXGOOD,ABS(LNGTHG(GELC))) + IF(LSEQNO.LT.LREG)LSEQNO=LREG + IS = LSEQNO-RELPG(GELC)+1 + ELSE +C LOOP FOR RELEVANT ELEMENTS THIS GEL +C only use mxgood characters from start of read (right end for these) +C + IREND = RELPG(GELC) - LNGTHG(GELC) + 1 + IF (MXGOOD.LT.MAXGEL) THEN + LSEQNO = IREND - MXGOOD + 1 + ELSE + LSEQNO = RELPG(GELC) + END IF + LSEQNO = MAX(LSEQNO,LREG) + IS = LSEQNO - RELPG(GELC) + 1 + N = ABS(LNGTHG(GELC)) + END IF + DO 70 I=IS,N + JJ = INDEXS(GEL(I),JSCORE) + JJJ = LWRAPS(LSEQNO,MAXGL2) + CHARS(JJ,ISS,JJJ) = CHARS(JJ,ISS,JJJ) + JSCORE + LSEQNO = LSEQNO + 1 +70 CONTINUE + IF(RNBR(GELC).EQ.0)GO TO 200 + GELC=RNBR(GELC) + LSEQNO=RELPG(GELC) + IF(LSEQNO.GT.RREG)GO TO 200 +C ENOUGH TO OUTPUT? + Y=LSEQNO-POSN + IF(Y.GE.MAXGEL)GO TO 210 + GO TO 50 +200 CONTINUE +C SET FLAG TO SHOW END REACHED + IEND=1 + LINLEN=MAXGEL + Y=RREG-POSN + IF(Y.LT.MAXGEL)LINLEN=Y+1 +210 CONTINUE +C SET POINTER TO SEQ1 + POSN1=POSN-1 +C PREPARE NEXT SECTION OF CHARS FOR OUTPUT + DO 230 I=1,LINLEN + JJJ = LWRAPS(POSN,MAXGL2) + LINOU1(I) = GTCONC(CHARS(1,1,JJJ),CHRSIZ,PERCD) + LINOU2(I) = GTCONC(CHARS(1,2,JJJ),CHRSIZ,PERCD) + DO 250 J=1,CHRSIZ + CHARS(J,1,JJJ)=0 + CHARS(J,2,JJJ)=0 +250 CONTINUE + POSN=POSN+1 +230 CONTINUE +C +C COMPARE STRANDS +C + DO 500 I=1,LINLEN +C WRITE(*,*)I,LINOU1(I),LINOU2(I) + POSN1=POSN1+1 + IF(LINOU1(I).EQ.LINOU2(I)) THEN + IF(LINOU1(I).EQ.'-') THEN + SEQ1(POSN1) = '3' + GO TO 500 + END IF + IF(LINOU1(I).EQ.'*') THEN + SEQ1(POSN1) = '3' + GO TO 500 + END IF + SEQ1(POSN1) = '0' + ELSE + IF((LINOU1(I).EQ.'*').AND.(LINOU2(I).EQ.'-')) THEN + SEQ1(POSN1) = '3' + GO TO 500 + END IF + IF((LINOU2(I).EQ.'*').AND.(LINOU1(I).EQ.'-')) THEN + SEQ1(POSN1) = '3' + GO TO 500 + END IF + IF((LINOU1(I).NE.'-').AND.(LINOU1(I).NE.'*')) THEN + SEQ1(POSN1) = '1' + IF((LINOU2(I).NE.'-').AND.(LINOU2(I).NE.'*')) + + SEQ1(POSN1) = '4' + GO TO 500 + END IF + IF((LINOU2(I).NE.'-').AND.(LINOU2(I).NE.'*')) THEN + SEQ1(POSN1) = '2' + IF((LINOU1(I).NE.'-').AND.(LINOU1(I).NE.'*')) + + SEQ1(POSN1) = '4' + GO TO 500 + END IF + END IF +500 CONTINUE + IF(POSN.GT.RREG)RETURN + IF((IEND.EQ.1).AND.(POSN.LE.RREG))GO TO 200 +C ANY MORE MAXGEL CHAR LENGTHS TO OUTPUT + Y=LSEQNO-POSN + IF(Y.LT.MAXGEL)GO TO 50 +C FINISHED COMPLETELY? + GO TO 210 + END +C SUMMER +C +C SUBROUTINE TO PRODUCE A CONSENSUS FROM LINED UP GEL READINGS + SUBROUTINE SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + 1SEQ1,IDIM1,GEL,LREG,RREG,IGELC,IDBSIZ,CHARS,CHRSIZ,MAXGL2, + +IDEVW,MAXGEL,IDM,PERCD) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),CHRSIZ + INTEGER LREG,RREG,LSEQNO,POSN,Y + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + INTEGER GELC + CHARACTER SEQ1(IDIM1) + INTEGER CHARS(CHRSIZ,MAXGL2) + CHARACTER MUNOTP,GTCONC + EXTERNAL MUNOTP,INDEXS,GTCONC,LWRAPS +C +C SET INITIAL VALUES + POSN=LREG + GELC=IGELC + LINLEN=MAXGEL + LSEQNO=RELPG(GELC) + IEND=0 + IPSEQ1=0 +C +C ZERO ARRAY + DO 40 I=1,MAXGL2 + DO 40 J=1,CHRSIZ + CHARS(J,I)=0 +40 CONTINUE +50 CONTINUE + CALL READW(IDEVW,GELC,GEL,MAXGEL) +C LOOP FOR RELEVANT ELEMENTS THIS GEL + N=ABS(LNGTHG(GELC)) + IF(LSEQNO.LT.LREG)LSEQNO=LREG + IS=(LSEQNO-RELPG(GELC))+1 +**************************** + IF(IDM.EQ.26)THEN + DO 51 I = IS,N + JJJ=(MOD(LSEQNO,MAXGL2)) + IF(JJJ.EQ.0)JJJ=MAXGL2 + CALL PCON1(GEL(I),CHARS(1,JJJ)) + LSEQNO = LSEQNO + 1 +51 CONTINUE + ELSE +**************************** + DO 70 I=IS,N + JJ = INDEXS(GEL(I),JSCORE) + JJJ = LWRAPS(LSEQNO,MAXGL2) + CHARS(JJ,JJJ) = CHARS(JJ,JJJ) + JSCORE + LSEQNO = LSEQNO + 1 +70 CONTINUE + END IF +C +C LOOK AT NEXT GEL TO RIGHT + IF(RNBR(GELC).EQ.0)GO TO 200 + GELC=RNBR(GELC) +C RESET LSEQNO + LSEQNO=RELPG(GELC) +C IS THIS OVER END? + IF(LSEQNO.GT.RREG)GO TO 200 +C ENOUGH TO OUTPUT? + Y=LSEQNO-POSN + IF(Y.GE.MAXGEL)GO TO 210 + GO TO 50 +200 CONTINUE +C SET FLAG TO SHOW END REACHED + IEND=1 +C NEED TO SUM AND OUTPUT + LINLEN=MAXGEL + Y=RREG-POSN + IF(Y.LT.MAXGEL)LINLEN=Y+1 +210 CONTINUE +C SUM NEXT SECTION OF CHARS + IF(IDM.EQ.26)THEN + DO 211 I = 1,LINLEN + IPSEQ1 = IPSEQ1 + 1 + SEQ1(IPSEQ1) = '-' + JJJ = MOD(POSN,MAXGL2) + IF(JJJ.EQ.0)JJJ = MAXGL2 + SEQ1(IPSEQ1) = MUNOTP(CHARS(1,JJJ)) + CHARS(1,JJJ) = 0 + POSN = POSN + 1 +211 CONTINUE + ELSE + DO 230 I=1,LINLEN + IPSEQ1=IPSEQ1+1 + ISUM=0 + JJJ = LWRAPS(POSN,MAXGL2) + SEQ1(IPSEQ1) = GTCONC(CHARS(1,JJJ),CHRSIZ,PERCD) + CALL FILLI(CHARS(1,JJJ),CHRSIZ,0) + POSN = POSN + 1 +230 CONTINUE + END IF +C +C +C ANY MORE TO OUTPUT? + IF(POSN.GT.RREG)RETURN + IF((IEND.EQ.1).AND.(POSN.LE.RREG))GO TO 200 +C ANY MORE MAXGLEL CHAR LENGTHS TO OUTPUT + Y=LSEQNO-POSN + IF(Y.LT.MAXGEL)GO TO 50 +C FINISHED COMPLETELY? + GO TO 210 + END + SUBROUTINE TPCHEK(PC,PG,L,N) + INTEGER PC(N),PG(N),L(N) +C AUTHOR RODGER STADEN +C IF OVERLAPPING BLOCKS ARE FOUND REMOVE THE SHORTER ONE +C THEN REMOVE LARGE GAPS AT ENDS (THOSE AS LARGE AS THE END BLOCK) + K1 = 2 +1 CONTINUE + DO 10 I = K1,N + J1 = I + IF(PC(I).LE.PC(I-1)) GO TO 20 + IF(PG(I).LE.PG(I-1)) GO TO 20 +10 CONTINUE +C REMOVE LARGE GAPS FROM ENDS +C THIS RULE OF THUMB COULD BE CHANGED TO USE A DIFFERENCE +C BETWEEN THE NUMBERS OF MISMATCHING CHARACTERS + IF(N.GT.1) THEN + K1 = PC(2) - PC(1) - L(1) + J1 = PG(2) - PG(1) - L(1) + IF(MAX(K1,J1).GT.L(1)) THEN + CALL ML(PC,PG,L,N,1) + N = N - 1 + END IF + IF(N.GT.1) THEN + K1 = PC(N) - PC(N-1) - L(N-1) + J1 = PG(N) - PG(N-1) - L(N-1) + IF(MAX(K1,J1).GT.L(N)) THEN + CALL ML(PC,PG,L,N,N) + N = N - 1 + END IF + END IF + END IF + RETURN +20 CONTINUE + IF(L(J1-1).GT.L(J1)) THEN + CALL ML(PC,PG,L,N,J1) + ELSE + CALL ML(PC,PG,L,N,J1-1) + END IF +C Until 25-11-90 next line was k1=j1 but this does not deal with all +C cases: when a line is deleted we must compare it with the previous +C one before dealing with the rest, because it could be left of that +C one as well! + K1 = MAX(2,J1-1) + N = N - 1 + GO TO 1 + END + SUBROUTINE WRITEN(IDEVN,N,NAME) + CHARACTER NAME*(*) + WRITE(IDEVN,REC=N)NAME + RETURN + END + SUBROUTINE WRITER(IDEVR,N,RELPG,LNGTHG,LNBR,RNBR) + INTEGER RELPG,RNBR + WRITE(IDEVR,REC=N)RELPG,LNGTHG,LNBR,RNBR + RETURN + END + SUBROUTINE WRITEW(IDEVW,N,GEL,MAXGEL) + CHARACTER GEL(MAXGEL) + WRITE(IDEVW,REC=N)GEL + RETURN + END + SUBROUTINE XHSAP(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,NCONTS,LLINOI,LINCNI,LREG,RREG, + +WINDOW,GWIND,LENCON,DEPTHP,DEPTHM, + +MARGL,MARGR,MARGB,MARGT,MAXOPT,ISXMAX,ISYMAX,KBIN,IDEV, + +KBOUT,GEL,GEL2,IDEV2,IDEV3,LINLEN,PERCD,MAXGEL,IDM, + +SEQ1,IDIM1,NGELS,TEMP3,CHRSIZ,MAXGL2,LINOU1,LINOU2, + +NOPT1,NOPT2,NOPT3, + +IHELPS,IHELPE,HELPF,IDEVH,MXGOOD) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),WINDOW,CHRSIZ,GWIND + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER MARGB(MAXOPT),MARGT(MAXOPT) + INTEGER RREG,DEPTHP,DEPTHM,STRAND,CHNRP1,HQN + INTEGER TEMP3(2,CHRSIZ,MAXGL2) + CHARACTER GEL(MAXGEL),GEL2(MAXGEL) + CHARACTER TERM,TUPPER,NAMARC*10,HELPF*(*) + CHARACTER SEQ1(IDIM1),LINOU1(MAXGEL),LINOU2(MAXGEL) + EXTERNAL NOPWIN,CWORLD,TUPPER,CHNRP1,HQN +C nopt1 = single contig +C nopt2 = all contigs +C nopt3 = scan +10 CONTINUE + LLINO = LLINOI + LINCON = LINCNI + LOCLR = 0 + LOCRR = 0 + CALL BPAUSE(KBIN,KBOUT,IOK) + CALL CLEARV + CALL XHAIRR(ISXMAX,ISYMAX,IX,IY,TERM,DBTDUX,DBTDUY) + CALL VT100M + INFLAG = HQN(TERM) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.3) RETURN + NOPT = NOPWIN(IY,MARGB,MARGT,MAXOPT) + TERM = TUPPER(TERM) + IF(NOPT.EQ.0) RETURN + IF(NOPT.EQ.NOPT3) THEN + IF(TERM.EQ.'S') THEN + XMIN = LREG + XMAX = RREG + X = CWORLD(IX,MARGL,MARGR,XMIN,XMAX) + LOCLR = MAX(LREG,NINT(X)-WINDOW) + LOCRR = MIN(RREG,NINT(X)+WINDOW-1) + IF(LOCLR.NE.0) THEN + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + + GEL,LLINO,LINCON,LOCLR,LOCRR,GEL2,I1,I2,0,I, + + IDBSIZ,IDEV,KBOUT, + + IDEV2,IDEV3,LINLEN,PERCD,MAXGEL,IDM) + GO TO 10 + END IF + END IF + IF((TERM.EQ.'N').OR.(TERM.EQ.'Z').OR.(TERM.EQ.'I')) GO TO 10 + END IF + IF(NOPT.EQ.NOPT1) THEN + STRAND = 1 + CALL FDPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON,STRAND,DEPTHP) + IF(DEPTHP.LT.0) RETURN + STRAND = -1 + CALL FDPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON,STRAND,DEPTHM) + IF(DEPTHM.LT.0) RETURN + YMAX = ISYMAX + YMIN = 0. + XMIN = LREG + XMAX = RREG + RINC = ISYMAX / (DEPTHP + DEPTHM + 2) + RMID =(DEPTHM+1) * RINC + X = CWORLD(IX,MARGL,MARGR,XMIN,XMAX) + Y = CWORLD(IY,MARGB(NOPT),MARGT(NOPT),YMIN,YMAX) + IF(TERM.EQ.'I') THEN + STRAND = 1 + YINC = RINC * STRAND + CALL IPLTC(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,STRAND, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + + ISXMAX,ISYMAX,RMID,YINC,DEPTHP,X,Y, + + KBOUT,IGEL,ICLOSE) + IF(ICLOSE.EQ.1) THEN + STRAND = -1 + YINC = RINC * STRAND + CALL IPLTC(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,STRAND, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + + ISXMAX,ISYMAX,RMID,YINC,DEPTHM,X,Y, + + KBOUT,IGEL,ICLOSE) + END IF + IF(ICLOSE.EQ.1) GO TO 10 + CALL READN(IDEV3,IGEL,NAMARC) + WRITE(IDEV,1006)NAMARC,IGEL,RELPG(IGEL),LNGTHG(IGEL) +1006 FORMAT + + ( ' Name ',A,' Number ',I4,' Rel. Posn. ',I7,' Length ',I5) + GO TO 10 + END IF + IF(TERM.EQ.'Z') THEN + STRAND = 1 + YINC = RINC * STRAND + CALL IPLTC(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,STRAND, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + + ISXMAX,ISYMAX,RMID,YINC,DEPTHP,X,Y, + + KBOUT,IGEL,ICLOSE) + IF(ICLOSE.EQ.1) THEN + STRAND = -1 + YINC = RINC * STRAND + CALL IPLTC(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,STRAND, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + + ISXMAX,ISYMAX,RMID,YINC,DEPTHM,X,Y, + + KBOUT,IGEL,ICLOSE) + END IF + IF(IGEL.EQ.0) GO TO 10 + CALL CLEARG + CALL PLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LINCON,IGEL, + + NCONTS,MARGL,MARGR,MARGB(NOPT2),MARGT(NOPT2),ISXMAX,ISYMAX) + LREG = MAX(1,RELPG(IGEL)-GWIND) + RREG = MIN(RELPG(LINCON),RELPG(IGEL)+GWIND) + LLINO = LNBR(LINCON) + LLINOI = LLINO + LINCNI = LINCON + LENCON = RREG - LREG + 1 + CALL FDEPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON, + + MARGL,MARGR,MARGB(NOPT1),MARGT(NOPT1),ISXMAX,ISYMAX) + GO TO 10 + END IF + IF(TERM.EQ.'S') THEN + LOCLR = MAX(LREG,NINT(X)-WINDOW) + LOCRR = MIN(RREG,NINT(X)+WINDOW-1) + IF(LOCLR.NE.0) THEN + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + + GEL,LLINO,LINCON,LOCLR,LOCRR,GEL2,I1,I2,0,I, + + IDBSIZ,IDEV,KBOUT, + + IDEV2,IDEV3,LINLEN,PERCD,MAXGEL,IDM) + GO TO 10 + END IF + END IF + IF(TERM.EQ.'N') THEN + LOCLR = MAX(LREG,NINT(X)-WINDOW) + LOCRR = MIN(RREG,NINT(X)+WINDOW-1) + IGEL = CHNRP1(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ, + + LLINO,LREG) + IF(LOCLR.NE.0) THEN + CALL LSTCON(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,IGEL, + + LOCRR,IDEV,IDEV3,NAMARC) + END IF + GO TO 10 + END IF + IF(TERM.EQ.'Q') THEN + CALL DBSCNP(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1, + + IDIM1,GEL,IDBSIZ,TEMP3,2,CHRSIZ,MAXGL2,IDEV2,LLINO, + + PERCD,MAXGEL,LINOU1,LINOU2,LREG,RREG, + + MARGL,MARGR,MARGB(NOPT3),MARGT(NOPT3),ISXMAX,ISYMAX, + + MXGOOD) + GO TO 10 + END IF + END IF + IF(NOPT.EQ.NOPT2) THEN + CALL IDPLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ, + + NCONTS,IX,IY,MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + + ISXMAX,ISYMAX,DBTDUX,DBTDUY, + + LINCON,IGEL,IS) + IF(IGEL.EQ.0) RETURN + IF(TERM.EQ.'Z') THEN + CALL CLEARG + CALL PLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LINCON,IGEL, + + NCONTS,MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX) + LREG = 1 + RREG = RELPG(LINCON) + LLINO = LNBR(LINCON) + LLINOI = LLINO + LINCNI = LINCON + LENCON = RREG - LREG + 1 + CALL FDEPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON, + + MARGL,MARGR,MARGB(NOPT1),MARGT(NOPT1),ISXMAX,ISYMAX) + GO TO 10 + END IF + IF(TERM.EQ.'Q') THEN + CALL CLEARG + CALL PLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LINCON,IGEL, + + NCONTS,MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX) + LREG = 1 + RREG = RELPG(LINCON) + LLINO = LNBR(LINCON) + LLINOI = LLINO + LINCNI = LINCON + LENCON = RREG - LREG + 1 + CALL FDEPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON, + + MARGL,MARGR,MARGB(NOPT1),MARGT(NOPT1),ISXMAX,ISYMAX) + CALL DBSCNP(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1, + + IDIM1,GEL,IDBSIZ,TEMP3,2,CHRSIZ,MAXGL2,IDEV2,LLINO, + + PERCD,MAXGEL,LINOU1,LINOU2,LREG,RREG, + + MARGL,MARGR,MARGB(NOPT3),MARGT(NOPT3),ISXMAX,ISYMAX, + + MXGOOD) + GO TO 10 + END IF + IF(TERM.EQ.'I') THEN + CALL READN(IDEV3,IGEL,NAMARC) + WRITE(IDEV,1006)NAMARC,IGEL,RELPG(IGEL),LNGTHG(IGEL) + GO TO 10 + END IF + IF(TERM.EQ.'S') THEN + LOCLR = MAX(1,IS-WINDOW) + LOCRR = MIN(RELPG(LINCON),IS+WINDOW-1) + LLINO = LNBR(LINCON) + IF(LOCLR.NE.0) THEN + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + + GEL,LLINO,LINCON,LOCLR,LOCRR,GEL2,I1,I2,0,I, + + IDBSIZ,IDEV,KBOUT, + + IDEV2,IDEV3,LINLEN,PERCD,MAXGEL,IDM) + END IF + GO TO 10 + END IF + IF(TERM.EQ.'N') THEN + LOCLR = MAX(1,IS-WINDOW) + LOCRR = MIN(RELPG(LINCON),IS+WINDOW-1) + LLINO = LNBR(LINCON) + IF(LOCLR.NE.0) THEN + CALL LSTCON(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,IGEL, + + LOCRR,IDEV,IDEV3,NAMARC) + END IF + GO TO 10 + END IF + END IF + END + INTEGER FUNCTION CLINNO(LNBR,IDBSIZ,NCONTS,IIN) +C AUTHOR: RODGER STADEN +C RETURNS CONTIG LINE NUMBER OR ZERO FOR ERROR + INTEGER LNBR(IDBSIZ) + CLINNO = 0 + N=IDBSIZ-NCONTS + DO 10 J=N,IDBSIZ-1 + IF(LNBR(J).EQ.IIN) THEN + CLINNO = J + RETURN + END IF +10 CONTINUE + END diff --git a/src/staden/dbsyscommon.f~ b/src/staden/dbsyscommon.f~ new file mode 100644 index 0000000..42a162b --- /dev/null +++ b/src/staden/dbsyscommon.f~ @@ -0,0 +1,5835 @@ +C DBSYS ROUTINES COMMON TO PRE AND POST .RD PROGRAMS +C 8-4-92 altered gtconc, summer,summar,dsplay +C 6-4-92 bug fix in autocn: i did not copy the correct elements +C for some cases where a better alignment was found: i was +C copying from jlefts instead of ilefts, jlc and jsense. +C Further sap routines are in dbsysold.f (pre .rd) and dbsysnew.f (post) +C the split was made by rs 23-1-91 +C 4-5-90 Change to getreg to allow escape +C 4-5-90 addition of graphics routines and changes to menus +C 9-5-90 added default gel reading: many changes +C 17-5-90 Fixed 3 bugs in screen editing: 1) rightjustified names +C caused problems; 2) beginnings of sequences starting at far +C right of lines where not seen; 3) lines with no numbers at +C the end of a contig (i.e. with <10 chars) were flagged +C as errors. Changes to ltype for 1, linlen for 2, dsplay for 3 +C 9-7-90 removed menu routines +C 20-8-90 changed gelid to add / to reading name because xsap did +C not return the INFLAG = 3 for the default +C 23-8-90 Changes to dbauto and autocn to deal with failures better +C Plus addition of calls to BUSY +C 9-11-90 Replaced call to radio with call to radion +C 19-11-90 Changed max match length in dbauto to maxglm+1 (was 50) +C 25-11-90 Very important bug fix in tpchek. Old versions could +C duplicate bits of working versions. +C 28-11-90 Modified slider to receive maxpg and maxpc and to allow exactly +C the requested number of matches at each end of the two +C sequences. +C Added two new options to dbauto: all gels to new contigs, all +C gels to contig 1; plus resurrected forbidding joins to allow +C sequences to be entered only into the contig the overlap best. +C Changed autocn to sort overlaps into order based on % mismatch +C (previously it saved the best two in any order) +C Minor change to dbstar +C 3-1-91 Discovered bug in dbopen: incorrect call to getint when the +C database is very old and needs values for the current format +C 21-1-91 GELID allowed illegal gel numbers to be returned! Fixed it. +C 22-1-91 Modified autocn, adism4,adism3 to give more info about +C overlaps, and to allow 10 overlaps. Modified dbopen to +C return version number, ditto dbstar +C 23-1-91 Split into dbsyscommon, dbsysold, dbsysnew +C 26-2-91 Improved overflow check in padcop +C 28-7-91 added extra parameter to quality calc: mxgood is the maximum +C reading length in which we have confidence, so only add this +C many chars from the start of each reading. Also changed the +C quality calc to make it the same as the consensus one. Made +C all characters have nonzero score and made lowercase = 100 +C 21-8-91 Changed arrfil to arrfim which does not display comments +C 22-8-91 Added routine to find contig line number given left gel (CLINNO) +C 8-11-91 Added fmt4lp which is used by "find internal joins" and would +C be useful elsewhere as a replacement for fmt4ln +C 18-11-91 New routine GETLN2 with returns gel number specified +C 27-2-92 Added use of ctonum to cfgel to deal with gel and vector +C being different case. +C 2-3-92 set FILNAM = ' ' for some calls to openf1 +C SUBROUTINE TO READ CHARACTER DATA FROM IDEV, REMOVE SPACES, FILL +C ARRAY AND RETURN NUMBER OF ELEMENTS USED. ANY LINES STARTING WITH +C A ; ARE TREATED AS COMMENTS + SUBROUTINE ARRFIM(IDEV,SEQNCE,J,KBOUT) +C 14-8-91 Added err= option to read, and set length to 0 if error found +C AUTHOR: RODGER STADEN + CHARACTER TEMP(80),SEQNCE(J) + CHARACTER SPACE,ENDCHR,TITCHR + SAVE ENDCHR,SPACE,TITCHR + DATA ENDCHR/'@'/ + DATA SPACE/' '/ + DATA TITCHR/';'/ + IDMX=J + J=0 +1 CONTINUE + READ(IDEV,1001,END=30,ERR=40)TEMP +1001 FORMAT(80A1) + IF(TEMP(1).EQ.TITCHR)THEN +C WRITE(KBOUT,1003)(TEMP(K),K=2,80) +C1003 FORMAT(' ',79A1) + GO TO 1 + END IF +10 CONTINUE + DO 20 I=1,80 + IF(TEMP(I).NE.SPACE)THEN + IF(TEMP(I).EQ.ENDCHR)RETURN + IF(J.EQ.IDMX)THEN + WRITE(KBOUT,1002)IDMX +1002 FORMAT( + + ' Too much data. Maximum possible', + + ' =',I6,', input stopped there') + RETURN + END IF + J=J+1 + SEQNCE(J)=TEMP(I) + END IF +20 CONTINUE + GO TO 1 +30 CONTINUE + RETURN + 40 CONTINUE + CALL ERROM(KBOUT,'Error reading file') + J = 0 + END +C ABEDIN +C +C ROUTINE TO EDIT THE DB USING A PADDED SEQ +C HAVE AN ARRAY SEQC2 LENGTH IDC OF PADDED SECTION OF CONTIG LINCON +C THE LEFT END OF THE PADDED CONTIG STARTS AT X +C THERE ARE ITOTPC PADS TO MAKE +C + SUBROUTINE ABEDIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,LINCON,X,SEQC2,ITOTPC,IDC,IDBSIZ,KBOUT,IDEVR,IDEVW, + +MAXGEL) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),X,POSN + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER SEQC2(IDC),GEL(MAXGEL),P + SAVE P + DATA P/','/ +C +C POINT TO CONTIG + POSN=X-1 +C POINT TO SEQC2 + IAT=0 +C COUNT PADS DONE + IDONE=0 +C LOOP FOR ALL SEQC2 + DO 100 J=1,IDC + POSN=POSN+1 + IAT=IAT+1 + IPAD=0 +C IS THIS A PADDING CHAR? + IF(SEQC2(IAT).NE.P)GO TO 100 +50 CONTINUE +C COUNT PADS + IPAD=IPAD+1 + IAT=IAT+1 + IF(SEQC2(IAT).EQ.P)GO TO 50 +C END OF THIS STRETCH OF PADS,DO INSERT +C HAVE IPAD INSERTS TO MAKE AT POSN + CALL PADCON(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,LINCON,POSN,IPAD,IDBSIZ,IDEVR,IDEVW,MAXGEL,KBOUT) +C MOVE POINTER TO CONTIG + POSN=POSN+IPAD +C COUNT PADS DONE + IDONE=IDONE+IPAD +C ANY MORE TO DO? + IF(IDONE.EQ.ITOTPC)GO TO 101 +100 CONTINUE +C ERROR SHOULD HAVE DONE ALL PADS + WRITE(KBOUT,1000) +1000 FORMAT(' Problem: some pads were not done!') +101 CONTINUE + END +C ACONS + SUBROUTINE ACONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,IDBSIZ,ISTART,ANS,LLINO,LREG,RREG,TEMP, + +CHRSIZ,MAXGL2,KBOUT, + +IDEVW,IFAIL,MAXGEL,IDM,PERCD) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),ANS,CHRSIZ + INTEGER LREG,RREG,X,Y,TEMP(CHRSIZ,MAXGL2) + CHARACTER SEQ1(IDIM1) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + CHARACTER NAMPRO*(*) +C WRITE(KBOUT,1000) +C1000 FORMAT(' CALCULATING A CONSENSUS') +C +C +C + CALL BUSY(KBOUT) + IFAIL=0 + IF(ANS.EQ.1)GO TO 150 + N=IDBSIZ-NCONTS + DO 110 I=N,IDBSIZ-1 + J=LNBR(I) + X=1 + Y=RELPG(I) + ISTART=ISTART+1 + IF((ISTART+19+Y).GT.IDIM1)THEN + WRITE(KBOUT,1009)IDIM1 +1009 FORMAT( + +' Database maximum consensus length(',I6,') exceeded',/, + +' calculation aborted') + IFAIL=1 + RETURN + END IF + CALL ADDTIT(SEQ1(ISTART),NAMPRO,J,ISTART) + CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + 1SEQ1(ISTART),Y,GEL,X,Y,J,IDBSIZ,TEMP,CHRSIZ,MAXGL2,IDEVW,MAXGEL, + +IDM,PERCD) + ISTART=ISTART+Y-1 +110 CONTINUE + RETURN +C SELECTED CONTIGS ONLY +150 CONTINUE + ISTART=ISTART+1 + IDIM11=RREG-LREG+1 + IF((ISTART+19+IDIM11).GT.IDIM1)THEN + WRITE(KBOUT,1009)IDIM1 + IFAIL=1 + RETURN + END IF + CALL ADDTIT(SEQ1(ISTART),NAMPRO,LLINO,ISTART) +C +C + CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + 1SEQ1(ISTART),IDIM11,GEL,LREG,RREG,LLINO,IDBSIZ,TEMP, + +CHRSIZ,MAXGL2, + 2IDEVW,MAXGEL,IDM,PERCD) + ISTART=ISTART+IDIM11-1 +C + RETURN + END + SUBROUTINE ADDTIT(SEQ1,NAMPRO,NGELS,IDIM1) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(20),NAMPRO*(*) + CHARACTER NUMS(10) + SAVE NUMS + DATA NUMS/'0','1','2','3','4','5','6','7','8','9'/ + CALL FILLC(SEQ1(2),18,'-') + SEQ1(1) = '<' + SEQ1(20) = '>' + IEND = INDEX(NAMPRO,'.') + N=NGELS + K=IEND+3 + DO 10 J=1,3 + N=MOD(N,10)+1 + NAMPRO(K:K)=NUMS(N) + N=NGELS/(10**J) + K=K-1 +10 CONTINUE + K = 18-IEND + K=K/2 + DO 20 I=1,IEND+3 + SEQ1(K)=NAMPRO(I:I) + K=K+1 +20 CONTINUE + IDIM1=IDIM1+20 + RETURN + END + SUBROUTINE ADISM1(SEQ,IDIM,GEL,IDIMG,SAVPS,SAVPG,IDSAV, + +CENDS,NENDS,IDCEND,MAXCON,ILEFTS,ILC,IPOSC,IPOSG,ISENSE, + +LLINO,IMATC, + +ISTRAN,KBOUT,MATCH) +C AUTHOR: RODGER STADEN +C NEW PARMS + INTEGER ILEFTS(2),ILC(2),IPOSC(2),IPOSG(2),ISENSE(2),LLINO(2) +CCCCCCCCCCCC + INTEGER CENDS(MAXCON) + INTEGER NENDS(MAXCON) + INTEGER SAVPS(IDSAV),SAVPG(IDSAV) + CHARACTER SEQ(IDIM),GEL(IDIMG),MATCH(IDIMG) +C +C EDITED 07-02-83 TO ALLOW FOR CASE WHERE A GEL OVERLAPS ADJACENT +C CONTIGS WITHIN THE LENGTH OF THE GEL. USE PARM THAT CONTAINS +C THE POSITION OF THE LEFT END OF THE NEXT CONTIG. SET TO VERY HIGH +C VALUE TO START + NEXTC=IDIM+1 +C SORT THE MATCHING WORDS INTO ASCENDING ORDER ON POSITION IN SEQ + CALL BUB2AS(SAVPS,SAVPG,IDSAV) +C LOOK FOR SEPERATE MATCHES + LEND=IDIMG-SAVPG(1)+SAVPS(1) +C COUNT NUMBER OF MATCHING CONTIGS + IMATC=IMATC+1 + CALL ADISM2(SEQ,IDIM,GEL,IDIMG,SAVPS(1), + 1SAVPG(1),CENDS,NENDS,IDCEND,MAXCON, + 1ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN,NEXTC,KBOUT, + 2MATCH) + DO 10 I=2,IDSAV + IF((SAVPS(I).LT.LEND).AND.(SAVPS(I).LT.NEXTC))GO TO 10 +C NEW MATCH, DISPLAY IT +C COUNT NUMBER OF MATCHING CONTIGS + IMATC=IMATC+1 + CALL ADISM2(SEQ,IDIM,GEL,IDIMG,SAVPS(I), + 1SAVPG(I),CENDS,NENDS,IDCEND,MAXCON, + 1ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN,NEXTC,KBOUT, + 2MATCH) +C +C RESET LEND + LEND=IDIMG-SAVPG(I)+SAVPS(I) +10 CONTINUE + RETURN + END +C +C ADISM2 +C ROUTINE TO DISPLAY MATCHES + SUBROUTINE ADISM2(SEQ,IDIM1,GEL,IDIMG,ISAVPS,SAVPG,CENDS,NENDS, + +IDCEND,MAXCON,ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN, + +NEXTC,KBOUT,MATCH) +C AUTHOR: RODGER STADEN +C NEW PARMS + INTEGER ILEFTS(2),ILC(2),IPOSC(2),IPOSG(2),ISENSE(2),LLINO(2) +CCCCCCCCCCC + CHARACTER SEQ(IDIM1),GEL(IDIMG),MATCH(IDIMG) + INTEGER SAVPS,SAVPG,CENDS(MAXCON) + INTEGER NENDS(MAXCON) +C EDITED 07-02-83 FOR NEXTC. SEE ADISM1. +C DELETE 20 FROM END OF CONSENSUS MATCH + SAVPS=ISAVPS-19 +C FIND CONTIG CONSENSUS ENDS + JJ=1 + DO 5 J=2,IDCEND + IF(SAVPS.GT.CENDS(J))GO TO 5 +C GONE PAST SO LAST IS THE ONE + JJ=J-1 + GO TO 6 +5 CONTINUE + JJ=IDCEND +6 CONTINUE +C SUBTRACT 1 FROM END + SAVPS=SAVPS-1 +C LENGTH FROM MATCH TO LEFT OF CONTIG + LCL=SAVPS-CENDS(JJ) +C RIGHT + LCR=CENDS(JJ+1)-ISAVPS-1 +C LEFT GEL + LGL=SAVPG-1 + LGR=IDIMG-SAVPG +C NEED MIN OF EACH PAIR + LL=MIN(LCL,LGL) + LR=MIN(LCR,LGR) +C LENGTH OF OVERLAP + LM=LR+LL+1 +C DISPLAY STARTS + ICL=ISAVPS-LL + IGL=SAVPG-LL + WRITE(KBOUT,1000)NENDS(JJ) +1000 FORMAT(' Match found with contig number =',I6) + CALL SQMTCH(SEQ(ICL),GEL(IGL),MATCH,LM) + L=ICL-CENDS(JJ)-19 + CALL FMT4LN(SEQ(ICL),GEL(IGL),MATCH,LM,L,IGL,KBOUT) +C UPDATE END OF NEXT CONTIG + NEXTC=CENDS(JJ+1)+20 + IF(IMATC.GT.2)RETURN + ILEFTS(IMATC)=CENDS(JJ)+20 + ILC(IMATC)=LCL+LCR+1 + IPOSC(IMATC)=LCL+1 + IPOSG(IMATC)=SAVPG + LLINO(IMATC)=NENDS(JJ) + ISENSE(IMATC)=1 + IF(ISTRAN.EQ.2)ISENSE(IMATC)=-1 + RETURN + END + SUBROUTINE ADISM3(ISAVPS,SAVPG,CENDS,NENDS, + +IDCEND,MAXCON,ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN, + +NEXTC,MAXC,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER ILEFTS(MAXC),ILC(MAXC),IPOSC(MAXC),IPOSG(MAXC) + INTEGER ISENSE(MAXC),LLINO(MAXC) + INTEGER SAVPS,SAVPG,CENDS(MAXCON) + INTEGER NENDS(MAXCON) + SAVPS=ISAVPS-19 + JJ=1 + DO 5 J=2,IDCEND + IF(SAVPS.GT.CENDS(J))GO TO 5 + JJ=J-1 + GO TO 6 +5 CONTINUE + JJ=IDCEND +6 CONTINUE + SAVPS=SAVPS-1 + LCL=SAVPS-CENDS(JJ) + LCR=CENDS(JJ+1)-ISAVPS-1 + NEXTC=CENDS(JJ+1)+20 + IF(IMATC.LE.MAXC) THEN + ILEFTS(IMATC)=CENDS(JJ)+20 + ILC(IMATC)=LCL+LCR+1 + IPOSC(IMATC)=LCL+1 + IPOSG(IMATC)=SAVPG + LLINO(IMATC)=NENDS(JJ) + ISENSE(IMATC)=1 + IF(ISTRAN.EQ.2)ISENSE(IMATC)=-1 + WRITE(KBOUT,1000)LLINO(IMATC),IPOSC(IMATC),ISTRAN, + + IPOSG(IMATC) + 1000 FORMAT + + (' Contig',I5,' position',I6,' matches strand',I2, + + ' at position',I5) + ELSE + CALL ERROM(KBOUT,'Warning: too many overlaps') + END IF + END + SUBROUTINE ADISM4(IDIM,IDIMG,SAVPS,SAVPG,IDSAV, + +CENDS,NENDS,IDCEND,MAXCON,ILEFTS,ILC,IPOSC,IPOSG,ISENSE, + +LLINO,IMATC,ISTRAN,MAXC,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER ILEFTS(MAXC),ILC(MAXC),IPOSC(MAXC),IPOSG(MAXC) + INTEGER ISENSE(MAXC),LLINO(MAXC) + INTEGER CENDS(MAXCON) + INTEGER NENDS(MAXCON) + INTEGER SAVPS(IDSAV),SAVPG(IDSAV) + NEXTC=IDIM+1 + CALL BUB2AS(SAVPS,SAVPG,IDSAV) + IMATC=IMATC+1 + CALL ADISM3(SAVPS(1),SAVPG(1),CENDS,NENDS,IDCEND,MAXCON, + + ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN,NEXTC,MAXC, + + KBOUT) + LEND=IDIMG-SAVPG(1)+SAVPS(1) + DO 10 I=2,IDSAV + IF((SAVPS(I).LT.LEND).AND.(SAVPS(I).LT.NEXTC))GO TO 10 + IMATC=IMATC+1 + CALL ADISM3(SAVPS(I),SAVPG(I),CENDS,NENDS,IDCEND,MAXCON, + + ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN,NEXTC,MAXC, + + KBOUT) + LEND=IDIMG-SAVPG(I)+SAVPS(I) +10 CONTINUE + IMATC = MIN(IMATC,MAXC) + RETURN + END + SUBROUTINE AEDIT(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LGEL,NCONT, + +GEL,MAXGEL,CON,IDC,IDEVW,IDEVR,LREG,RREG,KBOUT) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),CON(IDC) + INTEGER RREG,PC,PCA,PG + NG = LGEL + PG = RELPG(NG) + PC = LREG + NTT = 0 + NCT = 0 + NDT = 0 +10 CONTINUE +C WRITE(*,*)'GEL',NG + CALL READW(IDEVW,NG,GEL,MAXGEL) + LG = ABS(LNGTHG(NG)) + IF(PC.LT.LREG) PC = LREG + PCA = PC - LREG + 1 + IG = PC - PG + 1 + LC = MIN(LG,RREG-PC+1) +C WRITE(*,*)'PC,PG,IG,LG,PCA,LC',PC,PG,IG,LG,PCA,LC + CALL ET(GEL(IG),LG,CON(PCA),LC,NE) + NTT = NTT + NE + CALL EC(GEL(IG),LG,CON(PCA),LC,NE) + NCT = NCT + NE + CALL ED(GEL(IG),LG,CON(PCA),LC,ND) + NDT = NDT + ND + CALL WRITEW(IDEVW,NG,GEL,MAXGEL) + IF(ND.GT.0) THEN + K = LNGTHG(NG) + LNGTHG(NG) = ABS(LNGTHG(NG)) - ND + LNGTHG(NG) = SIGN(LNGTHG(NG),K) + CALL WRITER(IDEVR,NG,RELPG(NG),LNGTHG(NG),LNBR(NG),RNBR(NG)) + END IF + IF(RNBR(NG).NE.0) THEN + NG = RNBR(NG) + PG = RELPG(NG) + PC = PG + IF(PG.LE.RREG) GO TO 10 + END IF + CALL EDR(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LGEL,NCONT, + +CON,IDC,IDEVW,IDEVR,LREG) + WRITE(KBOUT,1000)NTT +1000 FORMAT(' Number of transpositions=',I6) + WRITE(KBOUT,1001)NCT +1001 FORMAT(' Number of changes =',I6) + WRITE(KBOUT,1002)NDT +1002 FORMAT(' Number of deletions =',I6) + END +C AJOIN2 +C COMPLETES JOIN AND RETURNS LENGTH OF NEW CONTIG IN LLINOR + SUBROUTINE AJOIN2(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +RELX,LLINOL,LLINOR,LNCONL,LNCONR,IDEVR) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNBR(IDBSIZ),RNBR(IDBSIZ),LNGTHG(IDBSIZ) + INTEGER RELX +C RELX IS THE POSITION OF THE JOINT +C LLINOL IS THE LEFT GEL NUMBER OF THE LEFT CONTIG +C LLINOR IS THE LEFT GEL OF THE RIGHT CONTIG +C LNCONL IS THE LEFT CONTIG LINE NUMBER +C LNCONR IS THE RIGHT CONTIG LINE NUMBER +C +C ADJUST ALL RELATIVE POSITIONS IN RIGHT CONTIG + N=LLINOR + RELPG(N)=RELX +50 CONTINUE + IF(RNBR(N).EQ.0)GO TO 60 + N=RNBR(N) + RELPG(N)=RELPG(N)+RELX-1 + GO TO 50 +60 CONTINUE +C +C FIX UP NEW GEL LINE FOR OLD LEFT OF RIGHT CONTIG + LNBR(LLINOR)=RNBR(LNCONL) +C FIX UP RIGHT GEL OF LEFT CONTIG + N=RNBR(LNCONL) + RNBR(N)=LLINOR +C MERGE WILL SORT OUT THE CORRECT NEIGHBOURS +C + CALL MERGE(RELPG,LNGTHG,LNBR,RNBR,LNCONL,IDBSIZ) +C MERGE DOES NOT WRITE TO DISK + N=LNBR(LNCONL) +65 CONTINUE +C WRITE(IDEVR,REC=N)RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N),LNBR(N),RNBR(N)) + N=RNBR(N) + IF(N.NE.0)GO TO 65 +C CONTIG LINES + X=RELPG(LNCONR)+RELX-1 +C LENGTH MAY NOT HAVE INCREASED! + IF(X.GT.RELPG(LNCONL))RELPG(LNCONL)=X +C SAVE LENGTH OF NEW CONTIG + RELX=RELPG(LNCONL) +C WRITE(IDEVR,REC=LNCONL)RELPG(LNCONL),LNGTHG(LNCONL),LNBR(LNCONL), +C 1RNBR(LNCONL) + CALL WRITER(IDEVR,LNCONL,RELPG(LNCONL),LNGTHG(LNCONL), + +LNBR(LNCONL),RNBR(LNCONL)) +C +C NOW MOVE ALL DATA DOWN TO DELETE OLD RIGHT END + N=IDBSIZ-NCONTS + M=LNCONR-N + IF(M.EQ.0)GO TO 80 + K=LNCONR + J=LNCONR-1 + DO 70 I=1,M + RELPG(K)=RELPG(J) + LNGTHG(K)=LNGTHG(J) + LNBR(K)=LNBR(J) + RNBR(K)=RNBR(J) +C WRITE(IDEVR,REC=K)RELPG(K),LNGTHG(K),LNBR(K),RNBR(K) + CALL WRITER(IDEVR,K,RELPG(K),LNGTHG(K),LNBR(K),RNBR(K)) + K=K-1 + J=J-1 +70 CONTINUE +80 CONTINUE + NCONTS=NCONTS-1 +C WRITE(IDEVR,REC=IDBSIZ)NGELS,NCONTS + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) +90 CONTINUE + RETURN + END +C SUBROUTINE AJOIN3 + SUBROUTINE AJOIN3(RELPG,IDBSIZ,LINCON,ITYPE,ISENSE,JOINT,IDIM22, + +KLASS,IOVER,KBOUT,PL,PR) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),LINCON(2),IDIM22(2) + INTEGER ITYPE(2),ISENSE(2),JOINT(2),PL(2),PR(2) +C +C CALC POSITIONS OF CONTIGS RELATIVE TO FIXED GEL + DO 20 I=1,2 +C R+ + IF((ITYPE(I).NE.-1).OR.(ISENSE(I).NE.1))GO TO 11 + PL(I)=-1*JOINT(I)+2 + PR(I)=PL(I)+RELPG(LINCON(I))-1 + GO TO 20 +C L+ +11 CONTINUE + IF((ITYPE(I).NE.1).OR.(ISENSE(I).NE.1))GO TO 12 + PL(I)=JOINT(I) + PR(I)=PL(I)+RELPG(LINCON(I))-1 + GO TO 20 +C R- +12 CONTINUE + IF((ITYPE(I).NE.-1).OR.(ISENSE(I).NE.-1))GO TO 13 + PR(I)=JOINT(I)+IDIM22(I)-1 + PL(I)=PR(I)-RELPG(LINCON(I))+1 + GO TO 20 +C L- +13 CONTINUE + PR(I)=IDIM22(I)-JOINT(I)+1 + PL(I)=PR(I)-RELPG(LINCON(I))+1 +20 CONTINUE +C LENGTH OF OVERLAP + IOVER=MIN(PR(1),PR(2))-MAX(PL(1),PL(2))+1 + WRITE(KBOUT,1002)IOVER +1002 FORMAT(' Length of overlap between the contigs=',I6) +C +C CLASS NUMBER 1-16 + KLASS=1 + IF(ITYPE(1).EQ.1)KLASS=KLASS+8 + IF(ISENSE(1).EQ.-1)KLASS=KLASS+4 + IF(ITYPE(2).EQ.1)KLASS=KLASS+2 + IF(ISENSE(2).EQ.-1)KLASS=KLASS+1 +C WRITE(KBOUT,1001)KLASS +C1001 FORMAT(' CLASS OF JOIN=',I6) + RETURN + END +C ALINE +C +C ROUTINE TO LINE UP 2 SEQS. +C IT SLIDES,REMOVES OVERLAPPING MATCHES, +C SORTS MATCHES INTO ASCENDING ORDER, THEN DOES DOES A TOPOLOGICAL +C CHECK, AND THEN PRODUCES 2 LINED UP SEQS WITH PADDING CHARS +C VARIABLES +C SEQ1 CONSENSUS +C SEQ2 GEL ORIGINAL IN CORRECT ORIENTATION +C SEQG2 ALIGNED GEL +C SEQC2 ALIGNED CONSENSUS +C SEQ3 SAVED GEL RAW DATA +C ISAV1,2,3 STORE MATCHES AND POSITIONS +C IDSAV NUMBER ISAV'S +C IDC LENGTH OF INPUT SEQ1 +C IDIM2 LENGTH OF INPUT SEQ2 +C IDOUT LENGTH OF OUTPUT ALIGNED SEQ1 +C IDIM2 LENGTH OF SEQ2 ON OUTPUT AFTER ALIGNMENT +C MINSLI MIN MATCH FOR SLIDING +C IFAIL FLAG TO SHOW IF ALIGNMENT FAILED DUE TO TOO +C MANY MISMATCHES OR TOPOLIGICAL CHECK OR TOO MANY OR TOO MANY +C PADDING CHARS. 1=FAIL,0=PASS +C + SUBROUTINE ALINE(SEQ1,SEQ2,SEQG2,SEQC2,ISAV1,ISAV2,ISAV3, + +IDSAV,IDC,IDIM2,IDOUT,IC1,IG1,MINSLI,JOINT, + +ITOTPC,ITOTPG,IFAIL,ITYPE,MAXPC,MAXPG,PERMAX,KBOUT,SEQ3,MAXGEL, + +PERCM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDC),SEQ2(IDIM2),SEQG2(IDOUT),SEQC2(IDOUT) + CHARACTER SEQ3(MAXGEL) + INTEGER ISAV1(IDSAV),ISAV2(IDSAV),ISAV3(IDSAV) + MINSLT=MINSLI +C SAVE SEQ2 + CALL SQCOPY(SEQ2,SEQ3,IDIM2) + CALL MSTLKL(SEQ3,IDIM2) + IFAIL=1 +C FIND MATCHES + IPP=IDSAV + CALL SLIDER(SEQ1,IDC,SEQ3,IDIM2,IC1,IG1,MAXPG,MAXPC,MINSLT, + +ISAV1,ISAV2,ISAV3,IPP) + IF(IPP.GT.IDSAV)RETURN + IF(IPP.LT.1)RETURN + CALL REMOVL(ISAV2,ISAV3,ISAV1,IPP) + CALL BUB3AS(ISAV2,ISAV3,ISAV1,IPP) +C DO TOPOLOGICAL CHECK + CALL TPCHEK(ISAV2,ISAV3,ISAV1,IPP) + IFAIL=0 + CALL LINEUP(SEQ2,SEQ1,SEQG2,SEQC2,IDC,IDIM2,IDOUT,ISAV3,ISAV2, + +ISAV1,IPP,ITOTPC,ITOTPG,JOINT,ITYPE,KBOUT,MAXGEL,IFAIL) + IF(IFAIL.NE.0)RETURN +C IDIM2 IS NOW LENGTH OF ALIGNED GEL + WRITE(KBOUT,1052)ITOTPC,ITOTPG +1052 FORMAT(' Padding in contig= ',I4,' and in gel= ',I4) + CALL DALIGN(SEQC2,SEQG2,SEQ3,MAXGEL,IDOUT,IDIM2,JOINT, + +ITYPE,PERCM,KBOUT,IFAIL) +C ARE ALL CHECKS OK? + IF(ITOTPC.GT.MAXPC)IFAIL=1 + IF(ITOTPG.GT.MAXPG)IFAIL=1 + IF(PERCM.GT.PERMAX)IFAIL=1 + END + SUBROUTINE ARCSER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN +C SEARCHES FOR ARCHIVE NAMES + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER NAME1*10,NAME2*10 +10 CONTINUE + L = 0 + CALL GTSTR('Archive name',' ',NAME1,L,KBOUT,KBIN,INFLAG) + IF(L.EQ.0) RETURN + CALL CCASE(NAME1,1) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.3) RETURN + IF(NAME1(1:1).EQ.' ') RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + DO 100 I=1,NGELS + CALL READN(IDEVN,I,NAME2) + IF(NAME1.EQ.NAME2) THEN + WRITE(KBOUT,1003)NAME2,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) +1003 FORMAT(' ',A,2X,I4,2X,I6,2X,I6,2X,I6,2X,I6/) + GO TO 10 + END IF +100 CONTINUE + WRITE(KBOUT,1004)NAME1 +1004 FORMAT(' ',A,' Not in database') + GO TO 10 + END + SUBROUTINE AUTOCN(SEQ1,IDIM,GEL,IDIMG,ILEFTS,ILC,IPOSC, + +IPOSG,ISENSE,LLINO,IMATC,IFCOMP,MINMAT,POSNS,WORDP,WORDN, + +CONST,LENGTH,LPOWRC,KBOUT,MATCH,MAXGEL,MAXGLM,GELCOP,GELN, + +SAVPS,SAVPG,SAVL,MAXSAV,CENDS,NENDS,MAXCON, + +SEQG2,SEQC2,SEQ4,IDOUT,IDIM22,ITOTPG,ITOTPC,JOINT,IFAIL, + +ITYPE,MAXPC,MAXPG,PERMAX,MINSLI,SEQG3,SEQC3,KFAIL) +C AUTHOR: RODGER STADEN +C changed 29-11-90 to make first in list of alignments the best + INTEGER ILEFTS(2),ILC(2),IPOSC(2),IPOSG(2),ISENSE(2),LLINO(2) + INTEGER POSNS(IDIM),GELN(MAXGLM),WORDP(LPOWRC),SAVPS(MAXSAV) + INTEGER SAVPG(MAXSAV),SAVL(MAXSAV) + INTEGER WORDN(LPOWRC) + CHARACTER GELCOP(MAXGLM),MATCH(MAXGLM) + INTEGER CENDS(MAXCON),NENDS(MAXCON) + INTEGER CONST(LENGTH) + CHARACTER SEQ1(IDIM),GEL(MAXGLM) +C + CHARACTER SEQG2(MAXGLM,2),SEQC2(MAXGLM,2),SEQ4(MAXGLM) + INTEGER IDOUT(2),IDIM22(2),ITOTPG(2),ITOTPC(2),JOINT(2) + INTEGER IFAIL(2),ITYPE(2) + PARAMETER (MAXC = 10) + CHARACTER SEQG3(MAXGLM),SEQC3(MAXGLM) + INTEGER JLEFTS(MAXC),JLC(MAXC),JPOSC(MAXC),JPOSG(MAXC) + INTEGER JSENSE(MAXC),JLLINO(MAXC) + REAL PERMIS(2) + IFAIL(1) = 1 + IFAIL(2) = 1 + KFAIL = 0 +C 23-8-90 Need to deal with failures in a better way. Problem is +C case where overlaps are found but fail to align. In future +C signal them with new variable KFAIL which will be nonzero +C if any alignment fails. +C 29-11-90 Changed sorting of overlaps so that the best is first in the +C list returned to caller. +C SAVE GEL + CALL SQCOPY(GEL,GELCOP,IDIMG) +C COUNT NUMBER OF CONTIGS THAT MATCH + IMATC=0 + IDCEND=MAXCON + CALL BUSY(KBOUT) + CALL FNDCON(SEQ1,IDIM,CENDS,NENDS,IDCEND,MAXCON,KBOUT) + CALL ENCO(SEQ1,IDIM,POSNS,CONST,LENGTH) + CALL ENCONA(POSNS,IDIM,WORDP,WORDN,LPOWRC,LENGTH) +1 CONTINUE + ISTRAN=1 +2 CONTINUE + CALL MSTLKL(GEL,IDIMG) + CALL ENCO(GEL,IDIMG,GELN,CONST,LENGTH) + IDSAV=MAXSAV + CALL CFGEL(GELN,IDIMG,POSNS,IDIM,WORDP,WORDN,LENGTH,LPOWRC, + +SAVPG,SAVPS,SAVL, + +IDSAV,SEQ1,GEL,MINMAT,IFCOMP,KBOUT) + IF(IFCOMP.NE.0)RETURN + IF(IDSAV.NE.0)THEN + CALL ADISM4(IDIM,IDIMG,SAVPS,SAVPG,IDSAV,CENDS,NENDS, + + IDCEND,MAXCON,JLEFTS,JLC,JPOSC,JPOSG,JSENSE,JLLINO, + + IMATC,ISTRAN,MAXC,KBOUT) + END IF + ISTRAN=ISTRAN+1 + IF(ISTRAN.EQ.2) THEN + CALL SQCOPY(GELCOP,GEL,IDIMG) + CALL SQREV(GEL,IDIMG) + CALL SQCOM(GEL,IDIMG) + GO TO 2 + END IF + CALL SQCOPY(GELCOP,GEL,IDIMG) + KSENSE = 0 + WRITE(KBOUT,*)'Total matches found',IMATC + IF(IMATC.EQ.0) THEN + IFAIL(1) = 0 + RETURN + END IF + JMATC = 0 + DO 100 I = 1,IMATC + IF(JSENSE(I).EQ.-1) THEN + IF(KSENSE.EQ.0) THEN + CALL SQREV(GEL,IDIMG) + CALL SQCOM(GEL,IDIMG) + KSENSE = 1 + END IF + END IF + JDIM22 = IDIMG + JDOUT = MAXGEL + IDSAV = MAXSAV + WRITE(KBOUT,*)'Trying to align with contig',JLLINO(I) + CALL ALINE(SEQ1(JLEFTS(I)),GEL,SEQG3,SEQC3, + + SAVPS,SAVPG,SAVL,IDSAV,JLC(I),JDIM22,JDOUT, + + JPOSC(I),JPOSG(I),MINSLI,JJOINT,JTOTPC,JTOTPG, + + JFAIL,JTYPE,MAXPC,MAXPG,PERMAX,KBOUT,SEQ4,MAXGEL,PERMS) + IF(JFAIL.EQ.0) THEN + JMATC = JMATC + 1 + IF(JMATC.EQ.1) THEN +C Save in elements 1 + CALL COPYM(JLEFTS(I),ILEFTS(1),JLC(I),ILC(1), + + JPOSC(I),IPOSC(1),JSENSE(I),ISENSE(1), + + JLLINO(I),LLINO(1),JJOINT,JOINT(1),JTOTPC, + + ITOTPC(1),JTOTPG,ITOTPG(1),JTYPE,ITYPE(1), + + JDOUT,IDOUT(1),JDIM22,IDIM22(1), + + SEQG3,SEQG2(1,1),SEQC3,SEQC2(1,1), + + PERMS,PERMIS(1)) + IFAIL(1) = 0 + ELSE IF(JMATC.EQ.2) THEN + IF(PERMS.LT.PERMIS(1)) THEN +C Better match so save in elements 1, so copy 1 to 2 first + CALL COPYM(ILEFTS(1),ILEFTS(2),ILC(1),ILC(2), + + IPOSC(1),IPOSC(2),ISENSE(1),ISENSE(2), + + LLINO(1),LLINO(2),JOINT(1),JOINT(2),ITOTPC(1), + + ITOTPC(2),ITOTPG(1),ITOTPG(2),ITYPE(1),ITYPE(2), + + IDOUT(1),IDOUT(2),IDIM22(1),IDIM22(2), + + SEQG2(1,1),SEQG2(1,2),SEQC2(1,1),SEQC2(1,2), + + PERMIS(1),PERMIS(2)) + IFAIL(2) = 0 +C Now save in 1 + CALL COPYM(JLEFTS(I),ILEFTS(1),JLC(I),ILC(1), + + JPOSC(I),IPOSC(1),JSENSE(I),ISENSE(1), + + JLLINO(I),LLINO(1),JJOINT,JOINT(1),JTOTPC, + + ITOTPC(1),JTOTPG,ITOTPG(1),JTYPE,ITYPE(1), + + JDOUT,IDOUT(1),JDIM22,IDIM22(1), + + SEQG3,SEQG2(1,1),SEQC3,SEQC2(1,1), + + PERMS,PERMIS(1)) + ELSE +C Save in element 2 + CALL COPYM(JLEFTS(I),ILEFTS(2),JLC(I),ILC(2), + + JPOSC(I),IPOSC(2),JSENSE(I),ISENSE(2), + + JLLINO(I),LLINO(2),JJOINT,JOINT(2),JTOTPC, + + ITOTPC(2),JTOTPG,ITOTPG(2),JTYPE,ITYPE(2), + + JDOUT,IDOUT(2),JDIM22,IDIM22(2), + + SEQG3,SEQG2(1,2),SEQC3,SEQC2(1,2), + + PERMS,PERMIS(2)) + IFAIL(2) = 0 + END IF + ELSE + IF(PERMS.LT.PERMIS(1)) THEN +C Better match so save in elements 1, so copy 1 to 2 first + CALL COPYM(ILEFTS(1),ILEFTS(2),ILC(1),ILC(2), + + IPOSC(1),IPOSC(2),ISENSE(1),ISENSE(2), + + LLINO(1),LLINO(2),JOINT(1),JOINT(2),ITOTPC(1), + + ITOTPC(2),ITOTPG(1),ITOTPG(2),ITYPE(1),ITYPE(2), + + IDOUT(1),IDOUT(2),IDIM22(1),IDIM22(2), + + SEQG2(1,1),SEQG2(1,2),SEQC2(1,1),SEQC2(1,2), + + PERMIS(1),PERMIS(2)) + IFAIL(2) = 0 +C Now save in 1 + CALL COPYM(JLEFTS(I),ILEFTS(1),JLC(I),ILC(1), + + JPOSC(I),IPOSC(1),JSENSE(I),ISENSE(1), + + JLLINO(I),LLINO(1),JJOINT,JOINT(1),JTOTPC, + + ITOTPC(1),JTOTPG,ITOTPG(1),JTYPE,ITYPE(1), + + JDOUT,IDOUT(1),JDIM22,IDIM22(1), + + SEQG3,SEQG2(1,1),SEQC3,SEQC2(1,1), + + PERMS,PERMIS(1)) + ELSE IF(PERMS.LT.PERMIS(2)) THEN +C Save in element 2 + CALL COPYM(JLEFTS(I),ILEFTS(2),JLC(I),ILC(2), + + JPOSC(I),IPOSC(2),JSENSE(I),ISENSE(2), + + JLLINO(I),LLINO(2),JJOINT,JOINT(2),JTOTPC, + + ITOTPC(2),JTOTPG,ITOTPG(2),JTYPE,ITYPE(2), + + JDOUT,IDOUT(2),JDIM22,IDIM22(2), + + SEQG3,SEQG2(1,2),SEQC3,SEQC2(1,2), + + PERMS,PERMIS(2)) + END IF + END IF + ELSE + KFAIL = 1 + END IF +100 CONTINUE + IMATC = MIN(2,JMATC) + END + SUBROUTINE BATIN(SEQ,MAXSEQ,KBIN,KBOUT,IDEV1,IDEV2,IDEV3, + +FILNAM,IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR RODGER STADEN + CHARACTER SEQ(MAXSEQ) + CHARACTER FILNAM*(*),HELPF*(*) + CHARACTER BASE(4),BKEY(4) + SAVE BASE,BKEY + DATA BASE/'A','C','G','T'/ + DATA BKEY/'N','M',',','.'/ + CALL CBASE(BASE,BKEY,IOK,ICBASE, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + FILNAM = ' ' + CALL OPENF1(IDEV1,FILNAM,1,IOK,KBIN,KBOUT, + +'File of file names', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + NGELS=0 +10 CONTINUE + IDIM=MAXSEQ + WRITE(KBOUT,1003) +1003 FORMAT(' Type data on lines of < 80 characters, finish with @'/) + CALL ARRFIM(KBIN,SEQ,IDIM,KBOUT) + IF(IDIM.GT.0)THEN + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,1,IOK,KBIN,KBOUT, + + 'File name for gel reading', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.EQ.0) THEN + IF(ICBASE.EQ.0) THEN + CALL CCASEA(SEQ,IDIM,2) + DO 5 J = 1,4 + CALL SUBS(SEQ,IDIM,BKEY(J),BASE(J)) +5 CONTINUE + END IF + CALL CCASEA(SEQ,IDIM,1) + CALL FMTDKN(IDEV2,SEQ,IDIM) + CLOSE(UNIT=IDEV2) + NGELS=NGELS+1 + WRITE(IDEV1,1002)FILNAM +1002 FORMAT( A) + END IF + END IF + CALL YESNO(IANS,'Type in another gel reading', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IANS.EQ.0) GO TO 10 + CALL YESNO(IANS,'List gel readings', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IANS.EQ.0) THEN + REWIND IDEV1 + DO 50 I=1,NGELS + IDIM=MAXSEQ + READ(IDEV1,1002)FILNAM + CALL OPENRS(IDEV2,FILNAM,IOK,LRECL,2) + IF(IOK.NE.0)THEN + WRITE(KBOUT,*)' Error opening file' + GO TO 50 + END IF + WRITE(IDEV3,1007)FILNAM +1007 FORMAT(' ',A) + CALL ARRFIM(IDEV2,SEQ,IDIM,KBOUT) + IF(IDIM.GT.0)CALL FMTDB(SEQ,IDIM,1,IDIM,60,IDEV3) + CLOSE(UNIT=IDEV2) +50 CONTINUE + END IF + CLOSE(UNIT=IDEV1) + WRITE(KBOUT,*)'Number of gel readings entered',NGELS + END +C BEDIN +C +C SUBROUTINE TO BED A NEW GEL INTO THE DATABASE. IT DISPLAYS +C THE JOIN AND ALLOWS CHANGES TO IT, THEN IT ALLOWS EDITING OF +C THE NEW GEL USING EDITG OR THE GELS IN THE DATABASE USING +C DBEDIT. ANY CHANGES CAN BE DISPLAYED USING DSPLAY. THIS SUBROUTINE +C RETURNS NOPT TO TELL OF THE OUTCOME OF ITS USE:3=OK,1=REJOIN,4=GIVE UP +C ON INPUT NOPT=2 MEANS LEFT END OVERLAP + SUBROUTINE BEDIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,GEL2,LINCON,LLINO,NOPT,X,IDBSIZ,IDEV,KBIN,KBOUT, + +IDEVR,IDEVW,IDEVN,LINLEN,PERCD, + +HELPS1,HELPE1,HELPS2,HELPE2,FILEH,IDEVH,MAXGEL,IDM) + CHARACTER FILEH*(*) + INTEGER HELPS1,HELPE1,HELPS2,HELPE2 +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),ANS + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + CHARACTER GEL2(MAXGEL) + PARAMETER (MAXPRM = 32) + CHARACTER PROMPT(5)*(MAXPRM) + INTEGER LREG,RIGHTM + INTEGER X,RREG +C SAVE NOPT FOR LEFT END OR RIGHT JOIN + NTYPE=NOPT + LREG=X + IF(NTYPE .EQ.2)LREG=1 + RREG=LREG+49 + IF(RREG.GT.RELPG(LINCON))RREG=RELPG(LINCON) + I1=1 + IF(NTYPE.EQ.2)I1=X + I2=I1+49 + IF(I2.GT.ABS(LNGTHG(NGELS)))I2=ABS(LNGTHG(NGELS)) + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR,GEL2, + +LLINO,LINCON,LREG,RREG,GEL,I1,I2,2,LLINOR,IDBSIZ,IDEV, + +KBOUT,IDEVW,IDEVN,LINLEN,PERCD,MAXGEL,IDM) + CALL YESNO(ANS,'Joint correct', + +HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.NE.0) THEN + NOPT = 1 + RETURN + END IF +20 CONTINUE + CALL BELL(1,KBOUT) +C DBMENU now defunct for sap - so we use RADION instead +C CALL DBMENU(2,JOPT,HELPS1,HELPE1,FILEH,IDEVH, +C +KBIN,KBOUT) + PROMPT(1) = 'Complete entry' + PROMPT(2) = 'Edit contig' + PROMPT(3) = 'Display' + PROMPT(4) = 'Edit new gel reading' + JOPT = 1 + CALL RADION('Enter options', PROMPT, 4, JOPT, IHELPS, + + IHELPE, FILEH, IDEVH, KBIN, KBOUT) +C GIVE UP + IF(JOPT.EQ.-1)THEN + CALL YESNO(ANS,'Quit', + + HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.EQ.0) THEN + NOPT = 4 + RETURN + END IF + END IF +C COMPLETE JOIN + IF(JOPT.EQ.1)THEN + CALL YESNO(ANS,'Complete entry', + + HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.NE.0) GO TO 20 + NOPT=3 + RETURN + END IF +100 CONTINUE + IF(JOPT.EQ.4)THEN +C EDIT NEW GEL + IDIM=ABS(LNGTHG(NGELS)) + CALL EDITGL(GEL,GEL2,IDIM,KBIN,KBOUT,MAXGEL, + + HELPS2,HELPE2,FILEH,IDEVH,1) + LNGTHG(NGELS)=SIGN(IDIM,LNGTHG(NGELS)) + END IF +200 CONTINUE + IF(JOPT.EQ.3)THEN +C DISPLAY + LLINO=LNBR(LINCON) + LEFTMN=X + RIGHTM=X+ABS(LNGTHG(NGELS))-1 + RIGHTM=MIN(RIGHTM,RELPG(LINCON)) + IF(NTYPE.EQ.2)THEN + LEFTMN=1 + RIGHTM=ABS(LNGTHG(NGELS))-X+1 + RIGHTM=MIN(RIGHTM,RELPG(LINCON)) + END IF + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LEFTMN, + + RIGHTM,LREG, + + RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + HELPS1,HELPE1,FILEH,IDEVH,IERR) + IF(IERR.NE.0) GO TO 20 + IF(NTYPE.EQ.2)GO TO 210 + I1=LREG-X+1 + IF(I1.GT.ABS(LNGTHG(NGELS)))GO TO 200 + I2=RREG-X+1 + IF(I2.GT.ABS(LNGTHG(NGELS)))GO TO 200 + GO TO 230 +210 CONTINUE + I1=X+LREG-1 + I2=X+RREG-1 + IF(I1.GT.ABS(LNGTHG(NGELS)))GO TO 200 + IF(I2.GT.ABS(LNGTHG(NGELS)))GO TO 200 +230 CONTINUE + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR,GEL2, + + LLINO,LINCON,LREG,RREG,GEL,I1,I2,2,LLINOR,IDBSIZ,IDEV, + + KBOUT,IDEVW,IDEVN,LINLEN,PERCD,MAXGEL,IDM) + LLINO=LNBR(LINCON) + END IF +300 CONTINUE + IF(JOPT.EQ.2)THEN +C EDIT DATABASE + IF(NTYPE.EQ.2)THEN + IMAXL=1 + IMAXR=ABS(LNGTHG(NGELS))-X+1 + ELSE IF(NTYPE.EQ.1)THEN + IMAXL=X + IMAXR=X+ABS(LNGTHG(NGELS))-1 + END IF + CALL DBEDIT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,GEL2, + + LINCON,IMAXL,IMAXR,IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW, + + HELPS2,HELPE2,FILEH,IDEVH,MAXGEL) + END IF + IF(JOPT.EQ.1)THEN + CALL HELP2(HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + END IF + GO TO 20 + END + SUBROUTINE BREAKC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW,IDEVN, + +IHELPS,IHELPE,IHELP1,IHELP2,FILEH,IDEVH,IOK) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER CLEN,CHAINL,GCLIN + EXTERNAL CLEN,CHAINL,GCLIN +C ROUTINE TO BREAK A CONTIG INTO 2 +C LEFT GEL OF NEW RIGHT CONTIG IS IR +C RIGHT GEL OF NEW LEFT CONTIG IS IL +C LEFT GEL OF OLD LEFT CONTIG IS ILO +C CONTIG LINE OF OLD CONTIG IS NCONTO +C CONTIG LINE OF NEW RIGHT CONTIG IS NCONTR +C CONTIG LINE OF NEW LEFT CONTIG IS NCONTO +C LENGTH OF OLD CONTIG IS LCONTO + IOK = 1 + NCONTR = IDBSIZ - NCONTS - 1 + IF(NCONTR.LE.NGELS) THEN + WRITE(KBOUT,*)'Insufficient space for new contig line.' + WRITE(KBOUT,*)'Increase database size with copy' + RETURN + END IF +10 CONTINUE + MN = 0 + MX = NGELS + IR = 0 + CALL GETINT(MN,MX,IR, + + 'Number of gel reading that will become a left end', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF(IVAL.LT.1) RETURN + IR = IVAL + IL = LNBR(IR) + IF(IL.EQ.0)THEN + WRITE(KBOUT,*)'Gel number',IR,' is already a left end' + GO TO 10 + END IF + ILO = CHAINL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,IR) + IF(ILO.EQ.0)THEN + WRITE(KBOUT,*) + +'Problem with this contig. Check logical consistency' + WRITE(KBOUT,*)'of database. Break not made' + RETURN + END IF + NCONTO = GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,ILO) + IF(NCONTO.EQ.0)THEN + WRITE(KBOUT,*)'No contig line for this contig. Check logical' + WRITE(KBOUT,*)'consistency of database. Break not made' + RETURN + END IF + LCONTO = RELPG(NCONTO) + IF(LCONTO.LT.1)THEN + WRITE(KBOUT,*)'Contig has zero length. Break not made' + RETURN + END IF + CALL CBREAK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,KBOUT,IDEVR,IDEVW,IDEVN,IR,IL,ILO,NCONTO,NCONTR,IOK) + END + SUBROUTINE CBREAK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,KBOUT,IDEVR,IDEVW,IDEVN,IR,IL,ILO,NCONTO,NCONTR,IOK) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER CLEN + EXTERNAL CLEN +C ROUTINE TO BREAK A CONTIG INTO 2 +C LEFT GEL OF NEW RIGHT CONTIG IS IR +C RIGHT GEL OF NEW LEFT CONTIG IS IL +C LEFT GEL OF OLD LEFT CONTIG IS ILO +C CONTIG LINE OF OLD CONTIG IS NCONTO +C CONTIG LINE OF NEW RIGHT CONTIG IS NCONTR +C CONTIG LINE OF NEW LEFT CONTIG IS NCONTO +C LENGTH OF OLD CONTIG IS LCONTO + IOK = 1 + NCONTS = NCONTS + 1 +C WRITE LAST LINE OF DB + WRITE(KBOUT,*)'Increasing number of contigs by 1' + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) +C MAKE NEW CONTIG A COPY OF OLD + RELPG(NCONTR) = RELPG(NCONTO) + LNGTHG(NCONTR) = LNGTHG(NCONTO) + LNBR(NCONTR) = IR + RNBR(NCONTR) = RNBR(NCONTO) + WRITE(KBOUT,*)'Writing new right contig line' + CALL WRITER(IDEVR,NCONTR,RELPG(NCONTR),LNGTHG(NCONTR), + +LNBR(NCONTR),RNBR(NCONTR)) +C NEED LENGTH FOR OLD LEFT CONTIG + RNBR(IL) = 0 + L = CLEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,IL) + IF(L.LT.1)THEN + WRITE(KBOUT,*) + + 'New left contig has zero length. Break not made' + RETURN + END IF + RELPG(NCONTO) = L + RNBR(NCONTO) = IL +C DO CONTIG LINE FOR NEW LEFT CONTIG + WRITE(KBOUT,*)'Writing new left contig line' + WRITE(KBOUT,*)'New length=',RELPG(NCONTO) + WRITE(KBOUT,*)'New right gel=',RNBR(NCONTO) + CALL WRITER(IDEVR,NCONTO,RELPG(NCONTO),LNGTHG(NCONTO), + +LNBR(NCONTO),RNBR(NCONTO)) +C DO GEL LINE FOR RIGHT GEL OF NEW LEFT CONTIG + WRITE(KBOUT,*)'Writing new right gel of left contig' + WRITE(KBOUT,*)'Gel number=',IL + CALL WRITER(IDEVR,IL,RELPG(IL),LNGTHG(IL), + +LNBR(IL),RNBR(IL)) +C DO GEL LINE FOR NEW RIGHT CONTIG + LNBR(IR) = 0 + WRITE(KBOUT,*)'Writing new left gel of right contig' + WRITE(KBOUT,*)'Gel number=',IR + CALL WRITER(IDEVR,IR,RELPG(IR),LNGTHG(IR), + +LNBR(IR),RNBR(IR)) +C NOW SHIFT + I = 1 - RELPG(IR) + WRITE(KBOUT,*)'Shifting gels in right contig by distance=',I + CALL SHIFTC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDEVR, + +IDBSIZ,IR,NCONTR,I) + WRITE(KBOUT,*)'Right contig length=',RELPG(NCONTR) + WRITE(KBOUT,*)'Break completed' + IOK = 0 + END +C BUBBL3 +C SUBROUTINE TO SORT INTEGER ARRAY (LIST) INTO ASCENDING ORDER +C + SUBROUTINE BUBBL3(LIST,LISTEL,LISTAL,IDIM) +C AUTHOR: RODGER STADEN + INTEGER LIST(IDIM),LISTEL(IDIM),LISTAL(IDIM) +C +C SET POINTERS TO ZERO + I=0 + J=0 +C +10 CONTINUE +C +C SET I=J IF WE HAVE JUST CORRECTLY POSITIONED AN ELEMENT + IF(J.GT.I)I=J +C +C INCREMENT POINTER TO NEXT ELEMENT + I=I+1 +C TEST FOR END OF ARRAY + IF(I.EQ.IDIM)RETURN +C +20 CONTINUE +C +C COMPARE ADJACENT ELEMENTS + IF(LIST(I).GE.LIST(I+1))GO TO 10 +C +C FIRST MOVE THIS ELEMENT? IF SO SET POINTER TO ITS INITIAL POSITION + IF(J.LT.I)J=I +C +C EXCHANGE ADJACENT ELEMENTS + ITEMP=LIST(I) + LIST(I)=LIST(I+1) + LIST(I+1)=ITEMP +C + ITEMP=LISTEL(I) + LISTEL(I)=LISTEL(I+1) + LISTEL(I+1)=ITEMP + ITEMP=LISTAL(I) + LISTAL(I)=LISTAL(I+1) + LISTAL(I+1)=ITEMP +C +C +C DECREMENT BACK THRU LIST WITH THIS ELEMENT + IF(I.GT.1)I=I-1 +C + GO TO 20 + END + SUBROUTINE CBASE(BASE,BKEY,IOK,ICBASE, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR RODGER STADEN + CHARACTER BASE(4),BKEY(4),HELPF*(*),NEW(4) + CHARACTER PROMPT*14 + SAVE PROMPT + DATA PROMPT/'Key for base '/ + IOK = 1 + CALL YESNO(ICBASE,'Use special keys for ACTG', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ICBASE.EQ.1) THEN + IOK = 0 + RETURN + ELSE IF(ICBASE.NE.0) THEN + RETURN + END IF + I = 1 +1 CONTINUE + IF(I.LT.5) THEN + PROMPT(14:14) = BASE(I) + L = 1 + CALL GETSTR(PROMPT,BKEY(I),NEW,4,L,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(INFLAG.EQ.2) RETURN + IF(NEW(1).EQ.';') THEN + WRITE(KBOUT,*)' ; is not allowed' + GO TO 1 + END IF + IF(L.NE.0) BKEY(I) = NEW(1) + I = I + 1 + GO TO 1 + END IF + CALL CCASEA(BKEY,4,2) + IOK = 0 + END + SUBROUTINE CCTA(SEQ,ID) + CHARACTER SEQ(ID),COM,AS + SAVE COM,AS + DATA COM/','/,AS/'*'/ + DO 10 I = 1,ID + IF(SEQ(I).EQ.COM) SEQ(I) = AS +10 CONTINUE + END + SUBROUTINE CEDT(KBIN,KBOUT, + +GELNOS,GELSTR,GELEND, + +MAXDB,GELNO,LINNO,MAXLIN,RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,NGELS,NCONTS,GEL,GEL2,MAXGEL,LINCON,PERCD,IDM, + +IHELPS,IHELPE,HELPF,IDEVH,IDEV,IDEV1,IDEV2,IDEV3,LINLEN, + +FILNAM,LLINO,IOK) + INTEGER RELPG(MAXDB),LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER LINEIN*80,GEL(MAXGEL),GEL2(MAXGEL) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),HELPF*(*) + INTEGER GELNOS(MAXDB),GELSTR(MAXDB),GELEND(MAXDB) + INTEGER GELNO(MAXLIN,2),LINNO(MAXLIN,2),RREG + WRITE(KBOUT,*)'Identify contig to edit' + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + +LLINO,IOK,IDBSIZ,KBIN,KBOUT,IDEV3, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,1,IOK,KBIN,KBOUT, + +'Name for temporary editing file', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + LREG = 1 + RREG = RELPG(LINCON) + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + +GEL,LLINO,LINCON,LREG,RREG,GEL2,I1,I2,0,I,IDBSIZ,IDEV,KBOUT, + +IDEV2,IDEV3,LINLEN,PERCD,MAXGEL,IDM) + CLOSE(UNIT=IDEV) + CALL CEDIT(FILNAM) + CALL YESNO(IWANT,'Put edited contig into database', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IWANT.NE.0) RETURN + CALL OPENRS(IDEV,FILNAM,IOK,LRECL,2) + IF(IOK.NE.0) RETURN + KGEL = LLINO + CALL BUSY(KBOUT) + CALL POSTC(GELNOS,GELSTR,GELEND,LINEIN, + +IOK,KBOUT,IDEV,MAXDB,GELNO,LINNO,MAXLIN,RELPG,LNGTHG, + +LNBR,RNBR,IDBSIZ,KGEL) + IF(IOK.NE.0) THEN + CLOSE(UNIT=IDEV) + RETURN + END IF + REWIND IDEV + CALL NEWDB(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,KBOUT,IDEV, + +IDEV1,IDEV2,IDEV3,MAXDB,GEL,MAXGEL,LINCON,KGEL,IOK) + CLOSE(UNIT=IDEV) + END +C +C CFGEL +C +C ROUTINE TO COMPARE A STRING OF WORD NUMBERS FOR A GEL WITH A SERIES +C OF ARRAYS REPRESENTING A CONSENSUS SEQUENCE. WE LOOK FOR OCCURENCES +C OF PAIRS OF WORDS (EACH WORD IS LENGTH CHARS LONG AND SO TOTAL MATCH IS +C 2*LENGTH CHARS LONG). THE ARRAYS SENT ARE OF SIZE 4**LENGTH (LE4) + SUBROUTINE CFGEL(GELN,IDIMG,POSNS,IDIM,WORDP,WORDN,LENGTH,LE4, + +SAVPG, + +SAVPS,SAVL,IDSAV,SEQ,GEL,MINMAT,IFAIL,KBOUT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),GEL(IDIMG) + INTEGER POSNS(IDIM),WORDP(LE4),SAVPS(IDSAV),SAVPG(IDSAV) + INTEGER GELN(IDIMG),SAVL(IDSAV) + INTEGER WORDN(LE4) + INTEGER W1,W2,PS1W1,PS1W2 + INTEGER CTONUM + EXTERNAL CTONUM +C bug fix 27-2-92: if sequence had different case matches would +C not be extended correctly because we look for identical characters +C switched to using ctonum + IDSAVM=IDSAV + IDSAV=0 +C LENGTH OF PAIR OF WORDS + LX2=2*LENGTH +C NUMBER OF PAIRS OF WORDS OF LENGTH LENGTH IN GEL + NW=IDIMG-(LX2-1) +C LOOP FOR EACH START POINT + DO 200 I=1,NW +C IS THIS WORD A ZERO? + W1=GELN(I) + IF(W1.EQ.0)GO TO 200 +C POINT TO NEXT WORD OF PAIR + W2=GELN(I+LENGTH) + IF(W2.EQ.0)GO TO 200 +C DOES W1 EXIST IN SEQ? + N1S1=WORDN(W1) + IF(N1S1.EQ.0)GO TO 200 + N2S1=WORDN(W2) + IF(N2S1.EQ.0)GO TO 200 +C BOTH EXIST, SO POINT TO THE FIRST + LENGTH + PS1W1=WORDP(W1)+LENGTH +C LOOP FOR ALL PAIRS + DO 50 J=1,N1S1 +C POINT TO FIRST W2 BECAUSE IT IS IN WORDP NOT POSNS + PS1W2=WORDP(W2) +C LOOP FOR THESE + DO 40 K=1,N2S1 +C ARE THIS PAIR LENGTH APART? + N=PS1W1-PS1W2 + IF(N.NE.0)GO TO 20 +C THEY ARE SO, IF REQUIRED LOOK FOR REST OF MATCH + LMAT=LX2 + IF(MINMAT.EQ.LX2)GO TO 15 + IPC=PS1W2+LENGTH + IPG=I+LX2 +16 CONTINUE + IF(IPG.GT.IDIMG)GO TO 15 + IF(IPC.GT.IDIM)GO TO 15 +C + IF(CTONUM(SEQ(IPC)).NE.CTONUM(GEL(IPG)))GO TO 15 + LMAT=LMAT+1 + IPC=IPC+1 + IPG=IPG+1 + GO TO 16 +15 CONTINUE +C IS MATCH LONG ENOUGH? + IF(LMAT.LT.MINMAT)GO TO 20 + IDSAV=IDSAV+1 + IF(IDSAV.LE.IDSAVM)GO TO 18 + WRITE(KBOUT,1000)IDSAVM +1000 FORMAT(' More than ',I6,' matches. Search aborted') + IFAIL=1 + RETURN +18 CONTINUE + SAVL(IDSAV)=LMAT + SAVPG(IDSAV)=I + SAVPS(IDSAV)=PS1W1-LENGTH +20 CONTINUE +C POINT TO NEXT W2 + PS1W2=POSNS(PS1W2) +40 CONTINUE +C ALL TRIED THIS PS1W1, TRY NEXT + PS1W1=POSNS(PS1W1-LENGTH)+LENGTH +50 CONTINUE +200 CONTINUE + IFAIL=0 + RETURN + END + INTEGER FUNCTION CHAINL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,IIN) +C AUTHOR: RODGER STADEN +C RETURNS CONTIG LEFT GEL NUMBER OR ZERO FOR ERROR + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + I = IIN + J = I + CHAINL = 0 +10 CONTINUE + IF(I.NE.0)THEN + J = I + I = LNBR(I) + IF(I.EQ.IIN)RETURN + GO TO 10 + END IF + CHAINL = J + END +C +C CHANGE +C +C ROUTINE TO EXCHANGE ALL THE CHARS IN A CHARACTER ARRAY USING +C A PAIR OF LOOKUP ARRAYS SENT BY CALLING PROG +C +C + SUBROUTINE CHANGE(SEQ,IDIM1,CHAR1,CHAR2,IDIM2,ELSE) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1) + CHARACTER CHAR1(IDIM2),CHAR2(IDIM2) + CHARACTER ELSE + DO 100 I=1,IDIM1 +C + DO 50 J=1,IDIM2 +C + IF(SEQ(I).NE.CHAR1(J))GO TO 50 +C MATCH SO EXCHANGE CHARS + SEQ(I)=CHAR2(J) + GO TO 100 +50 CONTINUE + SEQ(I)=ELSE +100 CONTINUE +C + RETURN + END + CHARACTER*1 FUNCTION CHARSL(I) + CHARACTER C*6 + SAVE C + DATA C/'ctag*-'/ + CHARSL = C(I:I) + END + CHARACTER*1 FUNCTION CHARSU(I) + CHARACTER C*6 + SAVE C + DATA C/'CTAG*-'/ + CHARSU = C(I:I) + END + INTEGER FUNCTION CHNRP(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LGEL,NCONT, + +LREG) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + I = LGEL + CHNRP = 0 +10 CONTINUE + IF(I.NE.0) THEN + IF(RELPG(I).LE.LREG) THEN + I = RNBR(I) + GO TO 10 + END IF + CHNRP = I + RETURN + END IF + END + INTEGER FUNCTION CHNRP1(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ, + +LGEL,LREG) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + I = LGEL + CHNRP1 = 0 +10 CONTINUE + IF(I.NE.0) THEN + IF(RELPG(I)+ABS(LNGTHG(I))-1.LT.LREG) THEN + I = RNBR(I) + GO TO 10 + END IF + CHNRP1 = I + RETURN + END IF + END + INTEGER FUNCTION CLEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,IIN) +C AUTHOR: RODGER STADEN +C RETURNS CONTIG LEFT GEL NUMBER OR ZERO FOR ERROR + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + I = IIN + CLEN= 0 + LEN = 0 +10 CONTINUE + IF(I.NE.0)THEN + LEN = MAX(LEN,(RELPG(I) + ABS(LNGTHG(I)) - 1)) + I = RNBR(I) + IF(I.EQ.IIN)RETURN + GO TO 10 + END IF + CLEN = LEN + END + SUBROUTINE CLIST(GELNO1,LINNO1,IGEL1,GELNO2,LINNO2, + +IGEL2,GELNOS,GELSTR,GELEND,IUNIQ1,IUNIQ,KBOUT,IOK) +C AUTHOR: RODGER STADEN + INTEGER GELNO1(IGEL1),GELNO2(IGEL2),GELNOS(IUNIQ1) + INTEGER GELSTR(IUNIQ1),GELEND(IUNIQ1) + INTEGER LINNO1(IGEL1),LINNO2(IGEL2) + EXTERNAL INLIST +C GELNOS === GEL NUMBERS (GELNOS) +C GELSTR === GEL START LINES +C GELEND === GEL END LINES +C GELNO === GEL NUMBERS PER STRIP +C LINNO === GEL LINE NUMBERS PER STRIP +C IGEL === NUMBER OF GELS PER STRIP +C LINENO === CURRENT LINE NUMBER +C +C WHICH GELS IN GELNO2 DO NOT APPEAR IN GELNO1 +C IE HAVE STARTED IN GELNO2 + DO 20 I=1,IGEL2 + MATCH=INLIST(GELNO1,IGEL1,GELNO2(I)) + IF(MATCH.EQ.0)THEN +C NO MATCH SO NEW +C PUT IN GELSTR + IUNIQ=IUNIQ+1 + GELNOS(IUNIQ)=GELNO2(I) + GELSTR(IUNIQ)=LINNO2(I) + END IF +20 CONTINUE +C WHICH GELS IN GELNO1 DO NOT APPEAR IN GELNO2 +C IE WHICH HAVE ENDED IN GELNO1 + DO 10 I=1,IGEL1 + MATCH=INLIST(GELNO2,IGEL2,GELNO1(I)) + IF(MATCH.EQ.0)THEN +C NO MATCH SO MUST HAVE ENDED +C WHERE IS IT STORED IN GELNOS? + MATCH=INLIST(GELNOS,IUNIQ,GELNO1(I)) + IF(MATCH.NE.0)THEN + GELEND(MATCH)=LINNO1(I) + GO TO 10 + END IF +C ERROR + WRITE(KBOUT,1000)GELNO1(I) +1000 FORMAT( ' Error: gel number ',I5, + + ' expected but not found in list') + IOK = 1 + RETURN + END IF +10 CONTINUE + IOK = 0 + RETURN + END +C +C CMPLMT +C +C SUBROUTINE TO REVERSE AND COMPLEMENT GELS AND DATA BASE +C THE POSITIONS OF THE RIGHT ENDS OF GELS ARE FIRST STORED +C IN RELPG THEN WE DO A BUBBLE SORT ON THESE POSITIONS +C UPDATING RELATIONSHIPS AS WE GO +C ALSO SEQUENCES ARE COMPLEMENTED, SIGNS OF LENGTH ARE +C MULTIPLIED BY -1 AND THE CONTIG LINE IS ALTERED + SUBROUTINE CMPLMT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LINCON,LLINO,GEL,IDBSIZ,KBOUT,IDEVR,IDEVW,MAXGEL) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + INTEGER X +C + WRITE(KBOUT,1000)LLINO +1000 FORMAT( ' Complementing contig',I6) +C CHAIN THRU AND PUT RIGHT ENDS IN RELPG + N=LLINO +10 CONTINUE + RELPG(N)=RELPG(N)+(ABS(LNGTHG(N)))-1 + IF(RNBR(N).EQ.0)GO TO 20 + N=RNBR(N) + GO TO 10 +20 CONTINUE +C +C NOW EFFECTIVELY BUBBLE SORT ON RELPG + N=RNBR(LINCON) + GO TO 22 +21 CONTINUE + N=NL + IF(I1.GT.0)N=I2 +22 CONTINUE + NL=LNBR(N) + IF(NL.EQ.0)GO TO 30 + I1=0 +23 CONTINUE + IF(RELPG(N).GE.RELPG(NL))GO TO 21 +C NOT IN CORRECT ORDER SO CHAIN ALONG UNTIL CORRECT,THEN COME +C BACK TO THIS POINT AND CONTINUE +C IF FIRST MOVE THIS LINE SET POINTER TO CURRENT POSITION + IF(I1.EQ.0)I2=N + I1=1 +C +C EXCHANGE NEIGHBOURS. CURRENTLY LOOKING AT N AND ITS LEFT +C NBR, AND THE LEFT NBR IS FURTHER RIGHT THAN N +C FIX UP POINTERS TO LEFT AND RIGHT OF THESE TWO + M=LNBR(NL) + IF(M.NE.0)RNBR(M)=N + M=RNBR(N) + IF(M.NE.0)LNBR(M)=NL + LNBR(N)=LNBR(NL) + LNBR(NL)=N + RNBR(NL)=RNBR(N) + RNBR(N)=NL +C CHAIN BACK THRU LIST WITH THIS LINE + N=RNBR(NL) + IF(N.EQ.0)GO TO 21 +C IE END MET + GO TO 23 +30 CONTINUE +C FINISH WITH LEFT END IN N +40 CONTINUE +C NOW REVERSE NBRS SO CHAIN BACK RIGHT + NL=RNBR(N) + IF(NL.EQ.0)GO TO 50 + RNBR(N)=LNBR(N) + LNBR(N)=NL + N=NL + GO TO 40 +50 CONTINUE +C NEED TO FIX UP NEW LEFT END + RNBR(N)=LNBR(N) + LNBR(N)=0 +C ALL POINTERS FIXED NOW DO RELATIVE POSITION +C FINISH WITH LEFT END IN N +C SO CHAIN BACK RIGHT +C SAVE RIGHT LINE NUMBER + NL=N + X=RELPG(N) +60 CONTINUE + RELPG(N)=1+(-1*(RELPG(N)-X)) + IF(RNBR(N).EQ.0)GO TO 70 + N=RNBR(N) + GO TO 60 +70 CONTINUE +C NOW FIX CONTIG LINE + LNBR(LINCON)=NL + RNBR(LINCON)=N +C WRITE NEW CONTIG LINE + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) +C WRITE(IDEVR,REC=LINCON)RELPG(LINCON),LNGTHG(LINCON),LNBR(LINCON), +C 1RNBR(LINCON) +C NOW REVERSE AND COMPLEMENT GELS + N=NL +80 CONTINUE +C READ(IDEVW,REC=N)GEL + CALL READW(IDEVW,N,GEL,MAXGEL) + M=ABS(LNGTHG(N)) + CALL SQREV(GEL,M) + CALL SQCOM(GEL,M) + CALL WRITEW(IDEVW,N,GEL,MAXGEL) +C WRITE(IDEVW,REC=N)GEL +C CHANGE SIGNS + LNGTHG(N)=-1*LNGTHG(N) +C WRITE NEW GEL LINE + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) +C WRITE(IDEVR,REC=N)RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) +C ANY MORE? + N=RNBR(N) + IF(N.NE.0)GO TO 80 +C NO MORE + RETURN + END +C CONSEN +C CALCULATES A CONSENSUS USING THE RULES OUTLINED IN THE DOCUMENTATION +C AND SUBROUTINE SUMMER +C UNIT IDEV IS USED FOR OUTPUT + SUBROUTINE CONSEN(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,IDBSIZ,TEMP,CHRSIZ,MAXGL2, + +KBIN,KBOUT,IDEVW,IDEV,NAMCON, + +IHELPS,IHELPE,FILEH,IDEVH,MAXGEL,IDM,PERCD,IDEVN,LLINO) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),ANS,CHRSIZ + INTEGER LREG,RREG,X,Y,TEMP(CHRSIZ,MAXGL2) + CHARACTER SEQ1(IDIM1) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + CHARACTER NAMPRO*(*) + CHARACTER NAMCON*(*) +100 CONTINUE + ISTART=1 + NAMCON = ' ' + CALL OPENF1(IDEV,NAMCON,1,IOK,KBIN,KBOUT, + +'Name for consensus file', + +IHELPS,IHELPE,FILEH,IDEVH) + IF(IOK.NE.0)RETURN + CALL YESNO(ANS,'Make consensus for whole database', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) RETURN + IF(ANS.EQ.1)GO TO 150 + N=IDBSIZ-NCONTS + CALL BUSY(KBOUT) + DO 110 I=N,IDBSIZ-1 + J=LNBR(I) + X=1 + Y=RELPG(I) + IF((ISTART+19+Y).GT.IDIM1)THEN + WRITE(KBOUT,1009)IDIM1 +1009 FORMAT( + + ' Maximum consensus length(',I6,') exceeded,',/, + + ' calculation aborted') + RETURN + END IF + CALL ADDTIT(SEQ1(ISTART),NAMPRO,J,ISTART) + CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + SEQ1(ISTART),Y,GEL,X,Y,J,IDBSIZ,TEMP,CHRSIZ,MAXGL2, + + IDEVW,MAXGEL,IDM,PERCD) + ISTART=ISTART+Y +110 CONTINUE + ISTART=ISTART-1 + CALL FMTDK(IDEV,SEQ1,ISTART) + GO TO 400 +150 CONTINUE + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LINCON,LLINO,IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) + IF(IERR.NE.0)GO TO 400 + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IERR) + IF(IERR.NE.0)GO TO 400 + IDIM2=RREG-LREG+1 + IF((ISTART+19+IDIM2).GT.IDIM1)THEN + WRITE(KBOUT,1009)IDIM1 + RETURN + END IF + CALL BUSY(KBOUT) + CALL ADDTIT(SEQ1(ISTART),NAMPRO,LLINO,ISTART) + CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ1(ISTART),IDIM2,GEL,LREG,RREG,LLINO,IDBSIZ,TEMP, + +CHRSIZ,MAXGL2,IDEVW,MAXGEL,IDM,PERCD) + ISTART=ISTART+IDIM2 +300 CONTINUE + CALL YESNO(ANS,'Select another contig', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.EQ.0) GO TO 150 + ISTART=ISTART-1 + CALL FMTDK(IDEV,SEQ1,ISTART) +400 CONTINUE + CALL YESNO(ANS,'Make another consensus', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.EQ.0)GO TO 100 + RETURN + END + SUBROUTINE COPYM(JLEFTS,ILEFTS,JLC,ILC, + +JPOSC,IPOSC,JSENSE,ISENSE,JLLINO,LLINO, + +JJOINT,JOINT,JTOTPC,ITOTPC,JTOTPG,ITOTPG, + +JTYPE,ITYPE,JDOUT,IDOUT,JDIM22,IDIM22, + +SEQG3,SEQG2,SEQC3,SEQC2,PERMS,PERMIS) + CHARACTER SEQG3(JDIM22),SEQG2(JDIM22),SEQC3(JDOUT),SEQC2(JDOUT) + ILEFTS = JLEFTS + ILC = JLC + IPOSC = JPOSC + IPOSG = JPOSG + ISENSE = JSENSE + LLINO = JLLINO + JOINT = JJOINT + ITOTPC = JTOTPC + ITOTPG = JTOTPG + ITYPE = JTYPE + IDOUT = JDOUT + IDIM22 = JDIM22 + CALL SQCOPY(SEQG3,SEQG2,JDIM22) + CALL SQCOPY(SEQC3,SEQC2,JDOUT) + PERMIS = PERMS + END + SUBROUTINE CRUNS(GEL,IFIRST,ILAST,RUN) + CHARACTER GEL*50,RUN*50,PAD + SAVE PAD + DATA PAD/'*'/ +C MARK RUNS IN THIS CONSENSUS SO THAT +C ATGGGCC BECOMES +C ** * + RUN(1:ILAST) = ' ' + DO 10 I = IFIRST+1,ILAST +C MUST ALLOW FOR THE EXTEMELY UNLIKELY CASE OF A RUN OF DASHES +C IN THE CONSENSUS + IF(GEL(I:I).NE.'-')THEN + IF(GEL(I:I).EQ.GEL(I-1:I-1)) RUN(I:I) = PAD + END IF +10 CONTINUE + END +C SUBROUTINE DALIGN +C +C COUNTS MISMATCHES AND DISPLAYS OVERLAP. + SUBROUTINE DALIGN(SEQC2,SEQG2,SEQ3,MAXGEL,IDOUT,IDIM2, + +JOINT,ITYPE,X,KBOUT,IFAIL) +C AUTHOR: RODGER STADEN + CHARACTER SEQC2(MAXGEL),SEQG2(MAXGEL),SEQ3(MAXGEL) + CHARACTER PAD,DASH + SAVE PAD,DASH + DATA PAD,DASH/',','-'/ + IENDG=1 + IENDC=JOINT +C ONLY LOOK AT OVERLAP WHICH IS FROM JOINT FOR LEFT TYPE JOIN + IF(ITYPE.EQ.1)THEN + IENDG=JOINT + IENDC=1 + END IF +100 CONTINUE +C LENGTH OF OVERLAP? + LG=IDIM2-IENDG+1 + LO=MIN(IDOUT,LG) +C SAVE RAW DATA + CALL SQCOPY(SEQG2,SEQ3,IDIM2) + CALL MSTLKL(SEQ3,IDIM2) + X=FLOAT(LO) + Y=X + K=IENDG+LO-1 +C POINT TO CONSENSUS + J=0 +C CHECK FOR OVERFLOW + IF(K.GT.MAXGEL)THEN + WRITE(KBOUT,1001) +1001 FORMAT( + +' Matching region too long for routine dalign. Alignment aborted') + IFAIL=1 + RETURN + END IF + DO 200 I=IENDG,K + J=J+1 + IF(SEQC2(J).EQ.SEQ3(I))GO TO 200 +C IF(SEQ3(I).EQ.DASH)GO TO 200 +C IF(SEQC2(J).EQ.DASH)GO TO 200 +C IF(SEQC2(J).EQ.PAD)GO TO 200 + X=X-1. +200 CONTINUE + X=(Y-X)*100./Y + WRITE(KBOUT,1000)X +1000 FORMAT(' Percentage mismatch after alignment = ',F4.1) + WRITE(KBOUT,1002) +1002 FORMAT(' Best alignment found') + CALL SQMTCH(SEQC2(1),SEQG2(IENDG),SEQ3,LO) + CALL FMT4LN(SEQC2(1),SEQG2(IENDG),SEQ3,LO,IENDC,IENDG,KBOUT) + IFAIL=0 + END +C DBCHEK +C TO CHECK THE SELF CONSISTENCY OF A DB +C ARE ALL NEIGHBOURS HOLDING HANDS OR DO SOME HAVE +C GELS WHICH DO NOT BELONG TO ANY CONTIG? OR WORSE BELONG TO MORE +C THAN ONE CONTIG? +C ARE THERE ANY LOOPS IN CONTIGS (THE WORST PROBLEM) +C ARE ALL RELATIVE POSITIONS IN SAME ORDER AS NEIGHBOURS? +C ARE THERE ANY CONTIGS OF LENGTH <1 ? +C ARE THERE ANY GELS OF ZERO LENGTH ? +C RETURN ERROR CODE OF ZERO FOR ALL OK, 1 FOR GELS NOT USED +C AND 2 FOR ALL OTHER ERRORS + SUBROUTINE DBCHEK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +TEMP,IERR,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER TEMP(IDBSIZ) + IERR=0 +C HAND HOLDING OK? + DO 100 I=1,NGELS + K=LNBR(I) + IF(K.EQ.0)GO TO 50 + IF(RNBR(K).EQ.I)GO TO 50 + WRITE(KBOUT,1000)I +1000 FORMAT(' Hand holding problem for gel reading',I6) + WRITE(KBOUT,1001)I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) + WRITE(KBOUT,1001)K,RELPG(K),LNGTHG(K),LNBR(K),RNBR(K) +1001 FORMAT(' ',5I6) + IERR=2 +50 CONTINUE + K=RNBR(I) + IF(K.EQ.0)GO TO 100 + IF(LNBR(K).EQ.I)GO TO 100 + WRITE(KBOUT,1000)I + WRITE(KBOUT,1001)I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) + WRITE(KBOUT,1001)K,RELPG(K),LNGTHG(K),LNBR(K),RNBR(K) + IERR=2 +100 CONTINUE +C +C ALL GELS IN CONTIGS OR IN MORE THAN ONE CONTIG? +C TEMP IS USED TO COUNT THE NUMBER OF TIMES EACH GEL IS USED + CALL FILLI(TEMP,IDBSIZ,0) + N=IDBSIZ-NCONTS +C COUNT NUMBER OF PASSES THRU LOOP 320 TO 300. IF THIS EXCEEDS +C THE DATABASE SIZE A LOOP HAS BEEN FOUND! + ICOUNT=0 + DO 300 I=N,IDBSIZ-1 + II=I + K=LNBR(I) + J=RNBR(I) + IF((K.NE.0).AND.(J.NE.0))GO TO 310 +C THIS CONTIG POINTS TO ZERO GEL NUMBER AS LEFT OR RIGHT END! + WRITE(KBOUT,1002)I +1002 FORMAT(' Contig',I4,' has zero gel numbers') + IERR=2 + GO TO 290 +310 CONTINUE + IF((LNBR(K).EQ.0).AND.(RNBR(J).EQ.0))GO TO 290 +C THIS LEFT END IS NOT A LEFT END OR THIS RIGHT END IS NOT A RIGHT END + WRITE(KBOUT,1004)I +1004 FORMAT(' The end gels of contig',I4,' have outward neighbours') + IERR=2 +290 CONTINUE +C DOES THE CONTIG HAVE SOME +VE LENGTH? + IF(RELPG(I).GT.0)GO TO 320 + WRITE(KBOUT,1010)I +1010 FORMAT(' The contig on line number',I4,' has zero length') + IERR=2 +320 CONTINUE + TEMP(K)=TEMP(K)+1 + ICOUNT=ICOUNT+1 + IF(ICOUNT.GT.IDBSIZ)GO TO 601 + K=RNBR(K) + IF(K.NE.0)GO TO 320 +300 CONTINUE + DO 400 I=1,NGELS + IF(TEMP(I).EQ.1)GO TO 390 + IF(TEMP(I).EQ.0)GO TO 410 + WRITE(KBOUT,1005)I,TEMP(I) +1005 FORMAT(' Gel number ',I6,' is used ',I6,' times') + IERR=2 + GO TO 400 +390 CONTINUE +C DOES THE GEL HAVE NONZERO LENGTH? (ONLY CHECK THOSE USED ONCE) + IF(LNGTHG(I).NE.0)GO TO 400 + WRITE(KBOUT,1011)I +1011 FORMAT(' Gel number',I6,' has zero length') + IERR=2 + GO TO 400 +410 CONTINUE + WRITE(KBOUT,1006)I +1006 FORMAT(' Gel number ',I6,' is not used') +C NEED TO INCREMENT THE ERROR COUNT NOT RESET IT TO A LOWER VALUE! + IF(IERR.LT.2)IERR=1 +400 CONTINUE +C ALL RELATIVE POSNS OK? + N=IDBSIZ-NCONTS + DO 500 I=N,IDBSIZ-1 + K=LNBR(I) + IF(K.EQ.0)GO TO 500 +510 CONTINUE + J=RNBR(K) + IF(J.EQ.0)GO TO 500 + IF(RELPG(K).GT.RELPG(J))GO TO 520 + K=J + GO TO 510 +520 CONTINUE + WRITE(KBOUT,1007)K,RELPG(K),J,RELPG(J) +1007 FORMAT(' Gel number',I6,' with position',I6, + + ' is the left neighbour of', + + /,' gel number',I6,' with position',I6) + K=J + IERR=2 + GO TO 510 +500 CONTINUE + IF(IERR.EQ.0)WRITE(KBOUT,1013) +1013 FORMAT(' Database is logically consistent') + RETURN +601 CONTINUE + IERR=2 + WRITE(KBOUT,1008)II +1008 FORMAT(' Loop in contig',I6,/, + +' No further checking done but gel numbers follow') + CALL FILLI(TEMP,IDBSIZ,0) + K=LNBR(II) +710 CONTINUE + TEMP(K)=TEMP(K)+1 + WRITE(KBOUT,1009)K +1009 FORMAT(' ',I6) + IF(TEMP(K).GT.1)RETURN + K=RNBR(K) + GO TO 710 + END + SUBROUTINE DBEDIT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,LINCON,IMAXL,IMAXR,IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW, + +IHELPS,IHELPE,FILEH,IDEVH,MAXGEL) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN +C SUBROUTINE TO EDIT GEL READINGS IN DATA BASE + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER POSN,Y,X + CHARACTER GEL(MAXGEL) + CHARACTER CHARS(80),PROMPT*32 + PARAMETER (MAXPRM = 32) + CHARACTER PRMPT(3)*(MAXPRM) + SAVE PROMPT + DATA PROMPT/'Characters for gel reading '/ +1 CONTINUE +C CALL BELL(1,KBOUT) +C CALL DBMENU(5,NOPT,IHELPS,IHELPE,FILEH,IDEVH, +C +KBIN,KBOUT) + PRMPT(1) = 'Insert' + PRMPT(2) = 'Delete' + PRMPT(3) = 'Change' + NOPT = 1 + CALL RADION('Edit options', PRMPT, 3, NOPT, IHELPS, + + IHELPE, FILEH, IDEVH, KBIN, KBOUT) + IF(NOPT.EQ.-1)RETURN + IF(NOPT.EQ.3)GO TO 200 +10 CONTINUE + MN = 1 + MX = RELPG(LINCON) + POSN = 0 + IF(IMAXL.GT.0) THEN + MN = IMAXL + MX = IMAXR + END IF + CALL GETINT(MN,MX,POSN, + +'Position to edit', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + IF(IVAL.EQ.0) GO TO 1 + POSN = IVAL +20 CONTINUE + MN = 1 + MX = 50 + NC = 1 + CALL GETINT(MN,MX,NC, + +'Number of characters', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + NC = IVAL +C NEED TO CHECK FOR DELETION TO END OF CONTIG + Y=POSN+NC + K=RELPG(LINCON)-POSN+1 + IF((NOPT.EQ.2).AND.(Y.GT.RELPG(LINCON)))NC=K +C NOW FIND FIRST CHAR THAT OVERLAPS REGION + LLINO=LNBR(LINCON) +30 CONTINUE + X=RELPG(LLINO)+ABS(LNGTHG(LLINO))-1 + IF(X.GE.POSN)GO TO 40 + LLINO=RNBR(LLINO) + GO TO 30 +40 CONTINUE + CALL READW(IDEVW,LLINO,GEL,MAXGEL) +C CALC POSN IN THIS GEL TO EDIT + K=POSN-RELPG(LLINO)+1 + IF(NOPT.EQ.2)GO TO 100 +50 CONTINUE + WRITE(PROMPT(28:),1005)LLINO +1005 FORMAT(I5) + LC = NC + CALL FILLC(CHARS,NC,'*') + CALL GETSTR(PROMPT,CHARS,CHARS,NC,LC,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) GO TO 1 + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 50 + END IF +C MOVE THE DATA RIGHT + M=ABS(LNGTHG(LLINO)) + LNGTHG(LLINO)=LNGTHG(LLINO)+SIGN(NC,LNGTHG(LLINO)) + N=ABS(LNGTHG(LLINO)) + IF(N.GT.MAXGEL)THEN + WRITE(KBOUT,2000)LLINO +2000 FORMAT( + +' Data pushed off end of gel',I5,' During insertion') + NM512=N-MAXGEL + N=MAXGEL +C SET M SO THAT THE DATA AT THE END IS LOST + M=M-NM512 + LNGTHG(LLINO)=SIGN(N,LNGTHG(LLINO)) + END IF + J=M-K+1 + DO 55 I=1,J + GEL(N)=GEL(M) + N=N-1 + M=M-1 +55 CONTINUE +C PERFORM THE INSERTION + IC=0 + DO 60 I=K,MIN(MAXGEL,K+NC-1) + IC=IC+1 +C DONT ALLOW SPACES - SET THEM TO *'S + IF(CHARS(IC).EQ.' ')CHARS(IC)='*' + GEL(I)=CHARS(IC) +60 CONTINUE + CALL WRITEW(IDEVW,LLINO,GEL,MAXGEL) + CALL WRITER(IDEVR,LLINO,RELPG(LLINO),LNGTHG(LLINO), + +LNBR(LLINO),RNBR(LLINO)) +65 CONTINUE + LLINO=RNBR(LLINO) + IF(LLINO.EQ.0)GO TO 70 + IF(RELPG(LLINO).GT.POSN)GO TO 70 + X=RELPG(LLINO)+ABS(LNGTHG(LLINO))-1 + IF(X.LT.POSN)GO TO 65 + GO TO 40 +70 CONTINUE +C INSERTS FINISHED SO NEED TO INCREMENT ALL THOSE GELS TO RIGHT + LLINO=LNBR(LINCON) +75 CONTINUE + IF(RELPG(LLINO).GT.POSN)GO TO 80 +76 CONTINUE + LLINO=RNBR(LLINO) + IF(LLINO.EQ.0)GO TO 90 + GO TO 75 +80 CONTINUE + RELPG(LLINO)=RELPG(LLINO)+(NC) + CALL WRITER(IDEVR,LLINO,RELPG(LLINO),LNGTHG(LLINO), + +LNBR(LLINO),RNBR(LLINO)) + GO TO 76 +90 CONTINUE + RELPG(LINCON)=RELPG(LINCON)+(NC) + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) + GO TO 1 +CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC +C DELETE +100 CONTINUE +C +C MOVE DATA TO LEFT + M=K+NC +C POSN TO MOVE TO + N=MAX(1,K) +C NUMBER TO MOVE + L=ABS(LNGTHG(LLINO))-M+1 +C NONE TO MOVE? + IF(L.GT.0) THEN + DO 160 I=1,L + GEL(N)=GEL(M) + N=N+1 + M=M+1 +160 CONTINUE + END IF +C NEDE TO FIX LENGTHS AND REL POSNS OF GELS EDITED +C 3 CLASSES GELS LOSING LEFT ENDS,RIGHT ENDS,CENTRES +C FOR LEFT ENDS K<1,RIGHT ENDS L<1 +C SO SET RELPG FOR THOSE LOSING LEFT ENDS + IF(K.LT.1)RELPG(LLINO)=POSN +C FIX LENGTHS + N=N-1 + IF(L.GT.0)LNGTHG(LLINO)=SIGN(N,LNGTHG(LLINO)) +C THOSE LOSING RIGHT ENDS + M=(POSN-RELPG(LLINO)) + IF(L.LT.1)LNGTHG(LLINO)=SIGN(M,LNGTHG(LLINO)) + CALL WRITER(IDEVR,LLINO,RELPG(LLINO),LNGTHG(LLINO), + +LNBR(LLINO),RNBR(LLINO)) + CALL WRITEW(IDEVW,LLINO,GEL,MAXGEL) +165 CONTINUE + LLINO=RNBR(LLINO) + IF(LLINO.EQ.0)GO TO 170 +C DOES IT HAVE DATA IN REGION? + X=POSN+(NC) + IF(RELPG(LLINO).GE.X)GO TO 170 + X=RELPG(LLINO)+ABS(LNGTHG(LLINO))-1 + IF(X.LT.POSN)GO TO 165 +C WITHIN + GO TO 40 +170 CONTINUE +C DELETIONS DONE SO DECREMENT ALL GELS TO RIGHT + LLINO=LNBR(LINCON) + X=POSN+(NC) +175 CONTINUE + IF(RELPG(LLINO).GE.X)GO TO 180 +176 CONTINUE + LLINO=RNBR(LLINO) + IF(LLINO.EQ.0)GO TO 190 + GO TO 175 +180 CONTINUE + RELPG(LLINO)=RELPG(LLINO)-(NC) + CALL WRITER(IDEVR,LLINO,RELPG(LLINO),LNGTHG(LLINO), + +LNBR(LLINO),RNBR(LLINO)) + GO TO 176 +190 CONTINUE + RELPG(LINCON)=RELPG(LINCON)-(NC) + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) + GO TO 1 +200 CONTINUE +C +CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC +C CHANGE + MN = 1 + MX = NGELS + LLINO = 0 + CALL GETINT(MN,MX,LLINO,'Gel number', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + IF(IVAL.EQ.0) GO TO 200 + LLINO = IVAL + WRITE(KBOUT,1022)RELPG(LLINO),LNGTHG(LLINO) +1022 FORMAT( ' This gels relative position =',I6,' and length =',I6) +220 CONTINUE + MN = RELPG(LLINO) + MX = RELPG(LLINO) + ABS(LNGTHG(LLINO)) - 1 + POSN = 0 + CALL GETINT(MN,MX,POSN, + +'Position to edit', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + IF(IVAL.EQ.0) GO TO 220 + POSN = IVAL + CALL READW(IDEVW,LLINO,GEL,MAXGEL) +225 CONTINUE + LC = 50 + NC = 0 + CALL GETSTR('New chararacters',' ',CHARS,LC,NC,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) GO TO 1 + IF(NC.EQ.0) GO TO 1 + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 225 + END IF + K=POSN-RELPG(LLINO)+1 +C COPY CHARS + DO 230 I=1,NC + IF(CHARS(I).EQ.' ')CHARS(I)='*' + GEL(K)=CHARS(I) + K=K+1 + IF(K.GT.MAXGEL) GO TO 231 +230 CONTINUE +231 CONTINUE + CALL WRITEW(IDEVW,LLINO,GEL,MAXGEL) + GO TO 1 + END +C DBPRNT +C PRINTS A DATABASE. IE ITS RELATIONSHIPS + SUBROUTINE DBPRNT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +IDEV,KBIN,KBOUT,IDEVN,LLINO, + +IHELPS,IHELPE,FILEH,IDEVH) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),LREG,RREG,ANS + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER NAMARC*10 + IF(NGELS.GT.0)CALL DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS, + +NCONTS,IDBSIZ,IDEV) + WRITE(IDEV,10011)NGELS,NCONTS +10011 FORMAT(' Number of gel readings',I5,' Number of contigs',I5) +20 CONTINUE + CALL YESNO(ANS,'Select contigs', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) RETURN + IF(ANS.EQ.0) GO TO 45 + N=IDBSIZ-NCONTS +25 CONTINUE + CALL YESNO(ANS,'Show gel readings in positional order', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) RETURN + IF(ANS.EQ.0)GO TO 41 + WRITE(IDEV,1009) +1009 FORMAT(' CONTIG LINES') + WRITE(IDEV,1000) +1000 FORMAT(' CONTIG LINE LENGTH ENDS'/ + +' LEFT RIGHT') + DO 30 I=N,IDBSIZ-1 + WRITE(IDEV,1007)I,RELPG(I),LNBR(I),RNBR(I) +30 CONTINUE +1007 FORMAT( ' ',12X,I4,2X,I6,10X,I6,2X,I6) + WRITE(IDEV,1008) +1008 FORMAT(' GEL LINES') + WRITE(IDEV,1001) +1001 FORMAT(' NAME NUMBER POSITION LENGTH NEIGHBOURS'/ + +' LEFT RIGHT') + DO 40 I=1,NGELS + CALL READN(IDEVN,I,NAMARC) + WRITE(IDEV,1006)NAMARC,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) +1006 FORMAT( ' ',A,2X,I4,2X,I7,2X,I5,2X,I6,2X,I6) +40 CONTINUE + RETURN +C +41 CONTINUE +C +C SORTED DATA + DO 43 I=N,IDBSIZ-1 + WRITE(IDEV,1021) +1021 FORMAT( ) + WRITE(IDEV,1000) + WRITE(IDEV,1007)I,RELPG(I),LNBR(I),RNBR(I) + J=LNBR(I) + WRITE(IDEV,1001) +42 CONTINUE + CALL READN(IDEVN,J,NAMARC) + WRITE(IDEV,1006)NAMARC,J,RELPG(J),LNGTHG(J),LNBR(J),RNBR(J) + J=RNBR(J) + IF(J.NE.0)GO TO 42 +43 CONTINUE + RETURN +45 CONTINUE +C SELECTED CONTIGS ONLY +C +C GET GEL NUMBER AND CONTIG NUMBER + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + +LLINO,IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) + IF(IERR.NE.0)RETURN + WRITE(IDEV,1009) + WRITE(IDEV,1000) + WRITE(IDEV,1007)LINCON,RELPG(LINCON),LNBR(LINCON),RNBR(LINCON) + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IERR) + IF(IERR.NE.0)RETURN + WRITE(IDEV,1008) + N=LLINO + WRITE(IDEV,1001) +46 CONTINUE + CALL READN(IDEVN,N,NAMARC) + WRITE(IDEV,1006)NAMARC,N,RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) + IF(RNBR(N).EQ.0)GO TO 48 + N=RNBR(N) + IF(RELPG(N).GT.RREG)GO TO 48 + GO TO 46 +48 CONTINUE + GO TO 45 + END + SUBROUTINE DBSCAN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1, + +IDIM1,GEL,IDBSIZ,TEMP3,ID1,CHRSIZ,MAXGL2,KBIN,KBOUT,IDEVW, + +IDEV,LINLEN,PERCD, + +IHELPS,IHELPE,FILEH,IDEVH,MAXGEL,LINOU1,LINOU2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEVN, + + LLINO,LINCON,LREG,RREG,MXGOOD) +C 28-7-91 added extra parameter mxgood: the max length of read +C we have confidence in + CHARACTER FILEH*(*) + PARAMETER (MAXPRM = 10) + CHARACTER PROMPT(2)*(MAXPRM) +C AUTHOR: RODGER STADEN + INTEGER RREG, RELPG(IDBSIZ),CHRSIZ + INTEGER LREG,TEMP3(ID1,CHRSIZ,MAXGL2),ANS + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),LINOU1(MAXGEL),LINOU2(MAXGEL) + CHARACTER SEQ1(IDIM1) + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LINCON,LLINO,IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) + IF(IERR.NE.0) RETURN + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IERR) + IF(IERR.NE.0) RETURN + IDIM2=RREG-LREG+1 + CALL SUMMAR(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,LREG,RREG,LLINO,PERCD,IDBSIZ, + +TEMP3,ID1,CHRSIZ,MAXGL2,IDEVW, + +MAXGEL,LINOU1,LINOU2,MXGOOD) + CALL DBSCSM(SEQ1(LREG),IDIM2,KBOUT) +160 CONTINUE + ANS = 1 + PROMPT(1) = 'List codes' + PROMPT(2) = 'Plot codes' + CALL RADION('Select results display mode',PROMPT,2,ANS, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANS.LT.1) RETURN + IF(ANS.EQ.1) THEN + CALL FMTDB(SEQ1,IDIM1,LREG,RREG,LINLEN,IDEV) + RETURN + ELSE + CALL PLTQ(SEQ1(LREG),IDIM2, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + END + SUBROUTINE DBSCNP(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1, + +IDIM1,GEL,IDBSIZ,TEMP3,ID1,CHRSIZ,MAXGL2,IDEVW,LLINO, + +PERCD,MAXGEL,LINOU1,LINOU2,LREG,RREG, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,MXGOOD) +C AUTHOR: RODGER STADEN + INTEGER RREG, RELPG(IDBSIZ),CHRSIZ + INTEGER LREG,TEMP3(ID1,CHRSIZ,MAXGL2) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),LINOU1(MAXGEL),LINOU2(MAXGEL) + CHARACTER SEQ1(IDIM1) + IDIM2=RREG-LREG+1 +C 28-7-91 added extra parameter mxgood: the max length of read +C we have confidence in + CALL SUMMAR(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,LREG,RREG,LLINO,PERCD,IDBSIZ, + +TEMP3,ID1,CHRSIZ,MAXGL2,IDEVW, + +MAXGEL,LINOU1,LINOU2,MXGOOD) + CALL PLTQ(SEQ1(LREG),IDIM2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END + SUBROUTINE DBSCSM(SEQ1,IDIM1,KBOUT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM1) + CHARACTER CODES(5) + REAL X(5) + SAVE CODES + DATA CODES/'0','1','2','3','4'/ + DO 50 J=1,5 + X(J)=0. +50 CONTINUE + DO 100 I=1,IDIM1 + DO 60 J=1,5 + IF(SEQ1(I).NE.CODES(J))GO TO 60 + X(J)=X(J)+1. + GO TO 61 +60 CONTINUE +61 CONTINUE +100 CONTINUE + SUM=0. + DO 130 J=1,5 + SUM=SUM+X(J) +130 CONTINUE + DO 140 J=1,5 + IF(SUM.NE.0)X(J)=X(J)*100./SUM +140 CONTINUE + WRITE(KBOUT,1001)X(1) +1001 FORMAT(' ',F6.2,'% OK on both strands and they agree(0)') + WRITE(KBOUT,1002)X(2) +1002 FORMAT(' ',F6.2,'% OK on plus strand only(1)') + WRITE(KBOUT,1003)X(3) +1003 FORMAT(' ',F6.2,'% OK on minus strand only(2)') + WRITE(KBOUT,1004)X(4) +1004 FORMAT(' ',F6.2,'% Bad on both strands(3)') + WRITE(KBOUT,1005)X(5) +1005 FORMAT(' ',F6.2,'% OK on both strands but they disagree(4)') + RETURN + END +C DBSTAT + SUBROUTINE DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +KBOUT) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + N=IDBSIZ-NCONTS + SUM=0. + DO 20 I=N,IDBSIZ-1 + SUM=SUM+RELPG(I) +20 CONTINUE + AV=SUM/NCONTS + WRITE(KBOUT,1020)SUM,AV +1020 FORMAT( ' Total contig length ',F10.0,' Average', + +' length ',F10.1) + SUMG=0. + DO 30 I=1,NGELS + SUMG=SUMG+FLOAT(ABS(LNGTHG(I))) +30 CONTINUE + AV=SUMG/SUM + WRITE(KBOUT,1021)SUMG +1021 FORMAT( ' Total characters in gel readings ',F10.0) + WRITE(KBOUT,1022)AV +1022 FORMAT + +( ' Average gel characters per consensus character ',F10.2) +99 CONTINUE + RETURN + END +C DELCON +C +C DELETES CONTIG FROM CONSENSUS SEQUENCE + SUBROUTINE DELCON(SEQ1,ILEFT,ILC,IDIM1) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM1) +C FIRST CHAR TO REPLACE + I1=ILEFT-20 +C FIRST CHAR TO MOVE + I2=ILEFT+ILC +C IS THIS RIGHTMOST CONTIG ANYWAY? + IF(I2.GT.IDIM1)GO TO 10 +C NUMBER TO MOVE + ID=IDIM1-I2+1 +C MOVE + CALL SQCOPY(SEQ1(I2),SEQ1(I1),ID) +C RESET LENGTH + IDIM1=I1+ID-1 + RETURN +10 CONTINUE +C RIGHTMOST CONTIG SO DONT MOVE + IDIM1=I1-1 +C + RETURN + END + SUBROUTINE DISMAT(SEQ,IDIM,GEL,IDIMG,SAVPS,SAVPG,IDSAV, + +CENDS,NENDS,IDCEND,MAXCON,KBOUT,MATCH) +C AUTHOR: RODGER STADEN + INTEGER CENDS(MAXCON) + INTEGER NENDS(MAXCON) + INTEGER SAVPS(IDSAV),SAVPG(IDSAV) + CHARACTER SEQ(IDIM),GEL(IDIMG),MATCH(IDIMG) +C EDIT 07-02-83 TO CHECK FOR CASE WHEN GEL OVERLAPS ADJACENT +C CONTIGS WITHIN THE LENGTH OF THE GEL! DONE BY HAVING A +C PARAMETER THAT STORES THE POSITION OF THE LEFT END OF THE +C NEXT CONTIG (IE THE ONE AFTER THE ONE THE CURRENT GEL OVERLAPS) +C SET IT TO A VERY LARGE VALUE INITIALLY + NEXTC=99999 +C SORT THE MATCHING WORDS INTO ASCENDING ORDER ON POSITION IN SEQ + CALL BUB2AS(SAVPS,SAVPG,IDSAV) +C LOOK FOR SEPARATE MATCHES + LEND=IDIMG-SAVPG(1)+SAVPS(1) +C WRITE(KBOUT,1000)SAVPG(1),SAVPS(1) + CALL DISMAU(SEQ,IDIM,GEL,IDIMG,SAVPS(1), + +SAVPG(1),CENDS,NENDS,IDCEND,MAXCON, + +NEXTC,KBOUT,MATCH) + DO 10 I=2,IDSAV + IF((SAVPS(I).LT.LEND).AND.(SAVPS(I).LT.NEXTC))GO TO 10 +C NEW MATCH, DISPLAY IT +C WRITE(KBOUT,1000)SAVPG(I),SAVPS(I) +C1000 FORMAT(' ',2I6) + CALL DISMAU(SEQ,IDIM,GEL,IDIMG,SAVPS(I), + +SAVPG(I),CENDS,NENDS,IDCEND,MAXCON, + +NEXTC,KBOUT,MATCH) +C RESET LEND + LEND=IDIMG-SAVPG(I)+SAVPS(I) +10 CONTINUE + RETURN + END +C +C DISMAU +C ROUTINE TO DISPLAY MATCHES +C EDITED 17-12-81 TO NOT SUBTRACT 1 FROM LCL AND LGR + SUBROUTINE DISMAU(SEQ,IDIM1,GEL,IDIMG,ISAVPS,SAVPG,CENDS,NENDS, + +IDCEND,MAXCON,NEXTC,KBOUT,MATCH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),GEL(IDIMG),MATCH(IDIMG) + INTEGER SAVPS,SAVPG,CENDS(MAXCON) + INTEGER NENDS(MAXCON) +C EDITED 07-02-83 FOR NEXTC (SEE DISMAT) +C DELETE 20 FROM END OF CONSENSUS MATCH + SAVPS=ISAVPS-19 +C FIND CONTIG CONSENSUS ENDS + JJ=1 + DO 5 J=2,IDCEND + IF(SAVPS.GT.CENDS(J))GO TO 5 +C GONE PAST SO LAST IS THE ONE + JJ=J-1 + GO TO 6 +5 CONTINUE + JJ=IDCEND +6 CONTINUE +C SUBTRACT 1 FROM END + SAVPS=SAVPS-1 +C LENGTH FROM MATCH TO LEFT OF CONTIG + LCL=SAVPS-CENDS(JJ) +C RIGHT + LCR=CENDS(JJ+1)-ISAVPS-1 +C LEFT GEL + LGL=SAVPG-1 + LGR=IDIMG-SAVPG +C NEED MIN OF EACH PAIR + LL=MIN(LCL,LGL) + LR=MIN(LCR,LGR) +C LENGTH OF OVERLAP + LM=LR+LL+1 +C DISPLAY STARTS + ICL=ISAVPS-LL + IGL=SAVPG-LL + WRITE(KBOUT,1000)NENDS(JJ) +1000 FORMAT(' Match found with vector number =',I6) + CALL SQMTCH(SEQ(ICL),GEL(IGL),MATCH,LM) + L=ICL-CENDS(JJ)-19 + CALL FMT4LN(SEQ(ICL),GEL(IGL),MATCH,LM,L,IGL,KBOUT) +C SAVE POSN OF END OF NEXT CONTIG + NEXTC=CENDS(JJ+1)+20 + RETURN + END + SUBROUTINE DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + +GEL,LLINOO,LINCON,LREG,RREG,GEL2,I1,IDIM,NOPT, + +LLINOR,IDBSIZ,IDEV,KBOUT,IDEVW,IDEVN,LINLEN,PERCD, + +MAXGEL,IDM) +C AUTHOR: RODGER STADEN + INTEGER CHRSIZ + PARAMETER (CHRSIZ = 6) + PARAMETER (IDC1 = CHRSIZ*100) + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER LREG,RREG,X,XLS2,XLS1,XRS2,XRS1,RREG2 + CHARACTER MATCH(100) + INTEGER CHARS(CHRSIZ,100),CHARS1(IDC1) + CHARACTER NAMARC*10 + CHARACTER GEL(MAXGEL) + CHARACTER GEL2(MAXGEL) + INTEGER RELPOS(10),RELPO2(10) + INTEGER GELC + INTEGER RP + INTEGER LSEQNO,RSEQNO + CHARACTER LINOUT(100) + CHARACTER MUNOTP + CHARACTER GTCONC + EXTERNAL GTCONC + EQUIVALENCE (CHARS1,CHARS) + CALL FILLI(CHARS1,IDC1,0) +C SET CONTIG NUMBER + ICON=1 + LLINO=LLINOO + NLEN=LINLEN/10 + LSEQNO=LREG + X=LINLEN+LSEQNO-1 + RSEQNO=MIN(RREG,X) +C SET LEFT GEL NUMBER FOR RIGHT CONTIG + LN2=LLINOR +C FIRST GEL NO IS LLINOO +C SET RREG FOR RIGHT CONTIG + RREG2=IDIM +C SET UP LSEQNO,RSEQNO FOR FOR NOPT=3 + XLS2=I1 + XRS2=RSEQNO-LSEQNO+XLS2 +9 CONTINUE +C IF RIGHT CONTIG SKIP NUMBER PRINTING + IF(ICON.EQ.2)GO TO 8 +C NEED TO KEEP LONGEST LINE LENGTH FOR OUTPUT OF CONSENSUS + IE=0 +C SETUP AND WRITE NUMBERS + RELPOS(1)=LSEQNO+9 + DO 5 I=2,NLEN + RELPOS(I)=RELPOS(I-1)+10 +5 CONTINUE + WRITE(IDEV,1023) + +(RELPOS(K),K=1,MIN(NLEN,MAX(1,(RSEQNO-LSEQNO+1)/10))) +1023 FORMAT( ' ',19X,10(I9,1X)) +C SET CURRENT LINE NUMBER +8 CONTINUE + GELC=LLINO +10 CONTINUE +C IS LEFT END OF CURRENT GEL >RREG + IF(RELPG(GELC).GT.RSEQNO)GO TO 200 +C ALSO NEED TO KNOW IF RIGHT END ON THIS LINE (IF .LT. NO DATA +C TO DISPLAY) + X=RELPG(GELC)+ABS(LNGTHG(GELC))-1 + IF(X.LT.LSEQNO)GO TO 190 + CALL READW(IDEVW,GELC,GEL,MAXGEL) + CALL FILLC(LINOUT,LINLEN,' ') + CALL READN(IDEVN,GELC,NAMARC) +C +C NEED TO KNOW HOW MANY CHARS TO COPY OVER TO OUTPUT LINE +C AND WHERE IN LINE TO PUT THEM +C CURRENT LINE LEFT END IS LSEQNO,RIGHT END RSEQNO +C SO LEFT START CHAR IS + X=MAX(LSEQNO,RELPG(GELC)) +C POSITION IN ARRAY LINE + LP=X-LSEQNO+1 +C RIGHT END CHAR IS + X=RELPG(GELC)+ABS(LNGTHG(GELC))-1 + X=MIN(RSEQNO,X) +C POSITION IN ARRAY LINE + RP=X-LSEQNO+1 +C LOOK FOR LONGEST LINE + IF(RP.GT.IE)IE=RP +C NEED LEFT START IN GEL + K=LSEQNO-RELPG(GELC)+1 + IF(K.LT.1)K=1 + NCOP=RP-LP+1 + IF(NCOP.GT.0)CALL SQCOPY(GEL(K),LINOUT(LP),NCOP) + N=LP+NCOP-1 + II=K-1 + IF(IDM.EQ.26)THEN + DO 50 I = LP,N + II = II + 1 + CALL PCON1(GEL(II),CHARS(1,I)) +50 CONTINUE + ELSE + DO 70 I=LP,N + II=II+1 + JJ = INDEXS(GEL(II),JSCORE) + CHARS(JJ,I) = CHARS(JJ,I) + JSCORE +70 CONTINUE + END IF + I=SIGN(GELC,LNGTHG(GELC)) + WRITE(IDEV,1020)I,NAMARC,(LINOUT(K),K=1,RP) +1020 FORMAT( ' ',I4,2X,A,2X,100A1) +C +190 CONTINUE +C NOW GET NEXT GEL TO RIGHT + GELC=RNBR(GELC) + IF(GELC.NE.0)GO TO 10 +200 CONTINUE +C CALC CONSENSUS AND WRITE IT + IF(IDM.EQ.26)THEN + DO 49 I = 1,LINLEN + LINOUT(I) = MUNOTP(CHARS(1,I)) + CHARS(1,I) = 0 +49 CONTINUE + ELSE + DO 230 I=1,LINLEN + LINOUT(I) = GTCONC(CHARS(1,I),CHRSIZ,PERCD) + CALL FILLI(CHARS(1,I),CHRSIZ,0) +230 CONTINUE + END IF + WRITE(IDEV,1019)(LINOUT(K),K=1,IE) +C IF REQUIRED WRITE COMPARISON GEL +C WHICH OPTION IN OPERATION? + IF(NOPT.EQ.2)GO TO 52 + IF(NOPT.NE.3)GO TO 250 +53 CONTINUE +C ALREADY DONE THIS LINE CONTIG2? + IF(ICON.EQ.2)GO TO 54 + ICON=2 +C NEED TO SAVE CONSENSUS FROM LEFT CONTIG + CALL SQCOPY(LINOUT,GEL2,IE) +C SAVE VALUES FROM LEFT CONTIG + XLS1=LSEQNO + XRS1=RSEQNO +C SAVE CURRENT LEFT GEL NUMBER + LN1=LLINO +C SET UP VALUES FOR RIGHT CONTIG + LSEQNO=XLS2 + RSEQNO=XRS2 +C SET LEFT GEL NUMBER + LLINO=LN2 +C GET NEXT GEL + GO TO 150 +54 CONTINUE +C SAVE CURRENT LEFT GEL NUMBER + LN2=LLINO +C SET VALUES FOR RIGHT CONTIG NEXT PASS + XLS2=XRS2+1 + XRS2=XLS2+LINLEN-1 + IF(XRS2.GT.RREG2)XRS2=RREG2 +C SET UP VALUES FOR LEFT CONTIG + LLINO=LN1 + ICON=1 + LSEQNO=XLS1 + RSEQNO=XRS1 +C SET DECREMENT FOR POINTER TO GEL2 + MMM=I1-1 +52 CONTINUE +1017 FORMAT(' NEWGEL ',100A1) +1018 FORMAT(' MISMATCH ',100A1) +1019 FORMAT(' CONSENSUS ',100A1) +1022 FORMAT( ' ',18X,100A1) + I2=I1+LINLEN-1 + IF(I2.GT.IDIM)I2=IDIM + IF(NOPT.EQ.2)WRITE(IDEV,1017)(GEL2(K),K=I1,I2) +C SET DECREMENT + IF(NOPT.EQ.2)MMM=0 +55 CONTINUE + CALL FILLC(MATCH,LINLEN,'*') + K=0 + DO 667 J=I1,I2 + K=K+1 + IF(GEL2(J-MMM).EQ.LINOUT(K))MATCH(K) = ' ' +667 CONTINUE + WRITE(IDEV,1018)(MATCH(K),K=1,IE) + RELPO2(1)=(I1)+9 + DO 240 I=2,NLEN + RELPO2(I)=RELPO2(I-1)+10 +240 CONTINUE + WRITE(IDEV,1023)(RELPO2(K),K=1,NLEN) + I1=I2+1 + I2=I2+LINLEN + IF(I2.GT.IDIM)I2=IDIM + IF(I1.GT.I2)RETURN +250 CONTINUE +C + WRITE(IDEV,1021) +1021 FORMAT( ) +C NEXT LINE LENGTH +C NEXT LENGTH IS OLD RIGHT +1 + LSEQNO=RSEQNO+1 +C NEW RIGHT IS LEFT +LENGTH + RSEQNO=LSEQNO+(LINLEN)-1 +C ARE WE OVER END OF REGION + IF(RSEQNO.GT.RREG)RSEQNO=RREG +C HAVE WE FINISHED REGION COMPLETELY + IF(RSEQNO.LT.LSEQNO) RETURN +C NOT FINISHED SO NEED TO FIND CURRENT LEFT GEL NO +C CURRENT LEFT GEL IS LLINO +C +150 CONTINUE +C NEED TO KNOW IF CURRENT LEFT GELS RIGHT END IS INSIDE REGION + X=RELPG(LLINO)+ABS(LNGTHG(LLINO))-1 + IF(X.GE.LSEQNO)GO TO 9 +C LOOK AT NEXT GEL TO RIGHT + LLINO=RNBR(LLINO) +C MAY HAVE GONE OVER END OF CONTIG????? + IF(LLINO.GT.0)GO TO 150 +300 CONTINUE + RETURN + END + SUBROUTINE EC(GEL,IDG,CON,IDC,K) + CHARACTER GEL(IDG),CON(IDC),CHARSL + EXTERNAL CHARSL,INDEXS + PARAMETER (IDASH = 6) + K = 0 + DO 10 I = 1,MIN(IDC,IDG) + JC = INDEXS(CON(I),J) + IF(JC.NE.IDASH) THEN + JG = INDEXS(GEL(I),J) + IF(JG.NE.JC) THEN + GEL(I) = CHARSL(JC) + K = K + 1 + END IF + END IF +10 CONTINUE +C WRITE(*,*)'NUMBER OF CHARS CORRECTED=',K + END + SUBROUTINE ED(GEL,IDG,CON,IDC,K) + CHARACTER GEL(IDG),CON(IDC),CHARSL + EXTERNAL CHARSL,INDEXS + K = 0 + DO 10 I = MIN(IDC,IDG),1,-1 + JC = INDEXS(CON(I),J) + IF(JC.EQ.5) THEN + IF(I.LT.IDG) CALL SQCOPY(GEL(I+1),GEL(I),IDG-I) + K = K + 1 + END IF +10 CONTINUE +C WRITE(*,*)'NUMBER OF CHARS DELETED=',K + END + SUBROUTINE EDITGL(GEL,CHARS,IDIMG,KBIN,KBOUT,MAXGEL, + +IHELPS,IHELPE,FILEH,IDEVH,ISTART) +C AUTHOR: RODGER STADEN + CHARACTER FILEH*(*) + INTEGER POSN,Y + CHARACTER GEL(MAXGEL),CHARS(MAXGEL) + PARAMETER (MAXPRM = 32) + CHARACTER PRMPT(3)*(MAXPRM) +1 CONTINUE +C CALL BELL(1,KBOUT) +C CALL DBMENU(5,NOPT,IHELPS,IHELPE,FILEH,IDEVH, +C +KBIN,KBOUT) + PRMPT(1) = 'Insert' + PRMPT(2) = 'Delete' + PRMPT(3) = 'Change' + NOPT = 1 + CALL RADION('Edit options', PRMPT, 3, NOPT, IHELPS, + + IHELPE, FILEH, IDEVH, KBIN, KBOUT) + IF(NOPT.EQ.1)THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(NOPT.EQ.-1)RETURN + IF(NOPT.EQ.3)GO TO 200 +C INSERT, DELETE + MN = ISTART + MX = ISTART + IDIMG + POSN = 0 + CALL GETINT(MN,MX,POSN, + +'Position to edit', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + IF(IVAL.EQ.0) GO TO 1 + POSN = IVAL + POSN=POSN-ISTART+1 + MN = 1 + MX = 50 + NC = 1 + CALL GETINT(MN,MX,NC, + +'Number of characters', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + NC = IVAL + Y=POSN+NC + K=IDIMG-POSN+1 + IF((NOPT.EQ.2).AND.(Y.GT.IDIMG))NC=K +C CALC POSN IN THIS GEL TO EDIT + K=POSN +C IS THIS DELETE? + IF(NOPT.EQ.4)GO TO 100 +50 CONTINUE + LC = NC + CALL FILLC(CHARS,NC,'*') + CALL GETSTR('characters to insert',CHARS,CHARS,NC,LC,KBOUT,KBIN, + +INFLAG) + IF(INFLAG.EQ.2) GO TO 1 + IF(IFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 50 + END IF +C MOVE THE DATA RIGHT + M=IDIMG + N=IDIMG+NC +C CHECK FOR OVERFLOW OF GEL + IF(N.GT.MAXGEL)THEN + WRITE(KBOUT,2000) +2000 FORMAT(' Data pushed off end of gel during insertion') +C HOW MUCH OVER? + NM512=N-MAXGEL + N=MAXGEL +C SET M SO THAT THE DATA AT THE END IS LOST + M=M-NM512 + END IF + J=M-K+1 + DO 55 I=1,J + GEL(N)=GEL(M) + N=N-1 + M=M-1 +55 CONTINUE +C PERFORM THE INSERTION + IC=0 + DO 60 I=K,MIN(MAXGEL,K+NC-1) + IC=IC+1 +C DONT ALLOW SPACES - SET THEM TO *'S + IF(CHARS(IC).EQ.' ')CHARS(IC)='*' + GEL(I)=CHARS(IC) +60 CONTINUE + IDIMG=MIN((IDIMG+NC),MAXGEL) + GO TO 1 +CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC +C DELETE +100 CONTINUE +C +C MOVE DATA TO LEFT + M=K+NC +C POSN TO MOVE TO + N=MAX(1,K) +C NUMBER TO MOVE + L=IDIMG-M+1 +C NONE TO MOVE? + IF(L.GT.0) THEN +C MOVE THE DATA + DO 160 I=1,L + GEL(N)=GEL(M) + N=N+1 + M=M+1 +160 CONTINUE + END IF +C RESET LENGTH + IDIMG=IDIMG-NC + GO TO 1 +200 CONTINUE +C +CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC +C CHANGE + MN = ISTART + MX = ISTART + IDIMG + POSN = 0 + CALL GETINT(MN,MX,POSN, + +'Position to edit', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 1 + IF(IVAL.EQ.0) GO TO 1 + POSN = IVAL +C ADD IN START POSITION HERE + POSN=POSN-ISTART+1 +225 CONTINUE + LC = 50 + NC = 0 + CALL GETSTR('New characters',' ',CHARS,LC,NC,KBOUT,KBIN,INFLAG) + IF(NC.EQ.0) GO TO 1 + IF(INFLAG.EQ.2) GO TO 1 + IF(IFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 225 + END IF +C CALC POSITION IN THIS GEL TO EDIT + K=POSN +C COPY CHARS + DO 230 I=1,NC + GEL(K)=CHARS(I) + K=K+1 +230 CONTINUE + GO TO 1 + END + SUBROUTINE EDR(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LGEL,NCONT, + +CON,IDC,IDEVW,IDEVR,LREG) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER CON(IDC) + INTEGER CHNRP + EXTERNAL CHNRP +C CHANGE RELATIVE POSITIONS FOR AE + ND = 0 + DO 10 I = IDC,1,-1 + IF(CON(I).EQ.'*') THEN + ND = ND + 1 + K = I + LREG - 1 + J = CHNRP(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LGEL,NCONT,K) + IF(J.NE.0) THEN + CALL SHIFTC(RELPG,LNGTHG,LNBR,RNBR,IDUM,JDUM,IDEVR, + + IDBSIZ,J,NCONT,-1) + END IF + END IF +10 CONTINUE +C WRITE(*,*)' NUMBER OF DELETIONS=',ND + END + SUBROUTINE ET(GEL,IDG,CON,IDC,K) + CHARACTER GEL(IDG),CON(IDC),CHARSL + EXTERNAL CHARSL,INDEXS + K = 0 + DO 10 I = 2,MIN(IDC,IDG) + JC = INDEXS(CON(I),J) + IF(JC.NE.6) THEN + JG = INDEXS(GEL(I),J) + IF(JG.NE.JC) THEN + JNG = INDEXS(GEL(I-1),J) + JNC = INDEXS(CON(I-1),J) + IF(JNC.NE.JNG) THEN + IF((JNG.EQ.JC).AND.(JNC.EQ.JG)) THEN + GEL(I) = CHARSL(JNG) + GEL(I-1) = CHARSL(JG) + K = K + 1 + END IF + END IF + END IF + END IF +10 CONTINUE +C WRITE(*,*)' NUMBER OF CHARS TRANSPOSED=',K + END + SUBROUTINE FDEPTH(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,LENCON, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG,DEPTHP,DEPTHM,STRAND + STRAND = 1 + CALL FDPTH(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,LENCON,STRAND,DEPTHP) + IF(DEPTHP.LT.0) RETURN + STRAND = -1 + CALL FDPTH(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,LENCON,STRAND,DEPTHM) + IF(DEPTHM.LT.0) RETURN + CALL PLTCON(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ, + +MARGL,MARGR,MARGB, + +MARGT,ISXMAX,ISYMAX,LGEL,LREG,RREG,DEPTHP,DEPTHM) + END + SUBROUTINE FDPTH(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,LENCON,STRAND,DEPTH) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG,DEPTH,STRAND + EXTERNAL NCDEP +C LREG = left contig position +C RREG = right '' '' +C LENCON = RREG-LREG+1 + I = LGEL + DEPTH = 0 +5 CONTINUE + IF(I.NE.0) THEN + IF((RELPG(I)+ABS(LNGTHG(I))-1).LT.LREG) THEN + I = RNBR(I) + GO TO 5 + END IF + ELSE + DEPTH = -1 + RETURN + END IF +C WRITE(*,*)'LGEL',LGEL +10 CONTINUE + IF(I.NE.0)THEN + IF(RELPG(I).LE.RREG) THEN + IF(SIGN(1,LNGTHG(I)).EQ.STRAND) THEN + K = RELPG(I) + ABS(LNGTHG(I)) -1 + DEPTH = MAX(NCDEP(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,I, + + STRAND,K),DEPTH) + END IF + I = RNBR(I) + GO TO 10 + END IF + END IF +C WRITE(*,*)'DEPTH',DEPTH + END +C FIND +C +C SUBROUTINE TO FIND THE FIRST OCCURENCE OF A GIVEN STRING +C IN A GIVEN ARRAY +C + SUBROUTINE FIND(SEQ,IDIM1,STRING,IDIM2,IMATCH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2),DASH + INTEGER PSEQ,PSTR + SAVE DASH + DATA DASH/'-'/ + PSEQ=0 + PSTR=1 + IMATCH=0 +C +100 CONTINUE +C +C PUT PSEQ TO WHERE THIS FAILED MATCH STARTED + PSEQ=PSEQ+1-PSTR +C +400 CONTINUE +C + PSTR=0 +C +500 CONTINUE +C +C POINT TO NEXT SEQ CHAR + PSEQ=PSEQ+1 +C TEST FOR END + IF(PSEQ.GT.IDIM1)GO TO 300 +C POINT TO NEXT STRING CHAR + PSTR=PSTR+1 +C TEST FOR DASH IN STRING + IF(STRING(PSTR).EQ.DASH)GO TO 450 +C TEST FOR DASH IN SEQ + IF(SEQ(PSEQ).EQ.DASH)GO TO 400 +C TEST FOR MATCH + IF(SEQ(PSEQ).NE.STRING(PSTR))GO TO 100 +C +450 CONTINUE +C +C TEST FOR END OF STRING IE. WHOLE STRING MATCH + IF(PSTR.LT.IDIM2)GO TO 500 +C HAVE MATCH. GET POINTER TO WHERE IT STARTED + IMATCH=PSEQ-IDIM2+1 +C +300 CONTINUE + RETURN + END + SUBROUTINE FMT4LP(SEQ1,SEQ2,IDIM,ISW,ISX,IDEV,NAME1,NAME2) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM),SEQ2(IDIM),MATCH(60),NAME1*(*),NAME2*(*) + INTEGER KL(6) + ISXX=ISX + ISWW=ISW + IE=0 +10 CONTINUE + IS=IE+1 + IE=IE+60 + IF(IE.GT.IDIM)IE=IDIM + N=IE-IS+1 + N=1+(N-1)/10 +C SET UP DECIMAL COUNTERS + DO 50 J=1,N + KL(J)=ISWW + ISWW=ISWW+10 +50 CONTINUE + WRITE(IDEV,1001)(KL(K),K=1,N) + WRITE(IDEV,1002)NAME1,(SEQ1(K),K=IS,IE) + IL = IE - IS + 1 + CALL SQMTCH(SEQ1(IS),SEQ2(IS),MATCH,IL) + WRITE(IDEV,1003)(MATCH(K),K=1,IL) + WRITE(IDEV,1002)NAME2,(SEQ2(K),K=IS,IE) +1002 FORMAT(2X,A,2X,6(10A1,1X)) + 1003 FORMAT(10X,6(10A1,1X)) +C SET UP DECIMAL COUNTERS + DO 60 J=1,N + KL(J)=ISXX + ISXX=ISXX+10 +60 CONTINUE + WRITE(IDEV,1001)(KL(K),K=1,N) +1001 FORMAT( 5X,6(I6,5X)) + IF(IE.LT.IDIM) GO TO 10 + END + SUBROUTINE FMTDB(SEQ1,IDIM,ISW,ISE,LINLEN,IDEV) +C NOTE SAME AS FMTSEP! +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM) + INTEGER KL(12) + ISWW=ISW-1 + IE=ISW-1 +1 CONTINUE + WRITE(IDEV,1003) +1003 FORMAT( ) +C SET UP DECIMAL COUNTERS + DO 50 J=1,LINLEN/10 + ISWW=ISWW+10 + KL(J)=ISWW +50 CONTINUE + IS=IE+1 + IE=IE+LINLEN + IF(IE.GT.ISE)IE=ISE + WRITE(IDEV,1001)(KL(KKK),KKK=1,MIN(IE-IS+1,LINLEN)/10) + WRITE(IDEV,1002)(SEQ1(K),K=IS,IE) +1002 FORMAT( ' ',12(10A1,1X)) +1001 FORMAT( ' ',12(5X,I6)) + IF(IE.EQ.ISE)RETURN + GO TO 1 + END + SUBROUTINE FNDCON(SEQ,IDIM,CENDS,NENDS,IDCEND,MAXCON,KBOUT) +C AUTHOR: RODGER STADEN +C STORES THEIR POSITIONS IN CENDS AND THEIR LEFT LINE NUMBERS IN NENDS + CHARACTER SEQ(IDIM),DC(3) + INTEGER CENDS(MAXCON) + INTEGER NENDS(MAXCON) + EXTERNAL IFROMC,INDEXA + IDCEND=0 + DO 10 I=1,IDIM + IF(SEQ(I).NE.'<')GO TO 10 + IDCEND=IDCEND+1 +C PUT POSITION OF LEFT END OF CONTIG IN CENDS + CENDS(IDCEND)=I + K = INDEXA(SEQ(I),20,'.') + IF(K.EQ.0) THEN + WRITE(KBOUT,*)'Error in contig title: no dot!' + IDCEND = 0 + RETURN + END IF + K = K + I +C K=I+11 + DO 5 J=1,3 + DC(J)=SEQ(K) + K=K+1 +5 CONTINUE + NENDS(IDCEND)=IFROMC(DC,3,KBOUT) +10 CONTINUE +C STORE POSITION OF LAST CHAR +1 TO SIMPLIFY DISPLAY ROUTINES + CENDS(IDCEND+1)=IDIM+1 + RETURN + END + INTEGER FUNCTION GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +IDBSIZ,IIN) +C AUTHOR: RODGER STADEN +C RETURNS CONTIG LINE NUMBER OR ZERO FOR ERROR + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + GCLIN = 0 + N=IDBSIZ-NCONTS + DO 10 J=N,IDBSIZ-1 + IF(LNBR(J).EQ.IIN) THEN + GCLIN = J + RETURN + END IF +10 CONTINUE + END + INTEGER FUNCTION GELID(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LLINO,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH,INFLAG) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN +C SEARCHES FOR ARCHIVE NAMES + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER NAME1*11,NAME2*10,NAME3*11,NFLAG + PARAMETER (NFLAG='/') + NAME3 = ' ' + IF(LLINO.NE.0) THEN + NAME3(1:1) = NFLAG + CALL READN(IDEVN,LLINO,NAME3(2:)) + END IF + GELID = 0 +10 CONTINUE + L = 0 + IF(LLINO.NE.0) L = 11 + CALL GTSTR('Contig identfier',NAME3, + +NAME1,L,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.4) RETURN + IF(INFLAG.EQ.3) THEN + GELID = LLINO + RETURN + END IF + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(NAME1(1:1).EQ.NFLAG) THEN + CALL CCASE(NAME1,1) + DO 20 I=1,NGELS + CALL READN(IDEVN,I,NAME2) + CALL CCASE(NAME2,1) + IF(NAME1(2:11).EQ.NAME2) THEN + GELID = I + RETURN + END IF +20 CONTINUE + WRITE(KBOUT,1004)NAME1(2:) +1004 FORMAT(' ',A,' is not in the database!') + ELSE + CALL RJST(NAME1) + READ(NAME1,1001,ERR=10,END=10)GELID +1001 FORMAT(I11) + IF((GELID.LT.1).OR.(GELID.GT.NGELS)) THEN + CALL ERROM(KBOUT,'Illegal gel reading number') + GO TO 10 + END IF + END IF + END + SUBROUTINE GELOUT(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ,NGELS, + +NCONTS,GEL,MAXGEL,IDEV3,IDEV4,IDEV5,IDEV1,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,FILNAM) + INTEGER RELPG(MAXDB) + INTEGER LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER GEL(MAXGEL) + CHARACTER FILNAM*(*),HELPF*(*) + CHARACTER NAMARC*10 + FILNAM = ' ' + CALL OPENF1(IDEV5,FILNAM,1,IOK,KBIN,KBOUT, + +'File for names of extracted gel readings', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + CALL YESNO(I,'Extract ends of contigs only', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(I.LT.0) RETURN + IF(I.EQ.0) GO TO 15 + DO 10 I=1,NGELS + L=ABS(LNGTHG(I)) + IF(L.GT.0)THEN + CALL READN(IDEV4,I,NAMARC) + WRITE(KBOUT,1002)NAMARC +1002 FORMAT(' ',A) + WRITE(IDEV5,1003)NAMARC +1003 FORMAT(A) + FILNAM = NAMARC + CALL OPENRS(IDEV1,FILNAM,IOK,LRECL,1) + IF(IOK.NE.0) GO TO 100 + CALL READW(IDEV3,I,GEL,MAXGEL) + IF(LNGTHG(I).LT.0)THEN + CALL SQREV(GEL,L) + CALL SQCOM(GEL,L) + END IF + CALL FMTDKN(IDEV1,GEL,L) + CLOSE(UNIT=IDEV1) + END IF +10 CONTINUE + RETURN +15 CONTINUE +C NUMBER OF LINES TO PROCESS + N=IDBSIZ-NCONTS + DO 20 I=N,IDBSIZ-1 + JL=LNBR(I) + JR=RNBR(I) + CALL READN(IDEV4,JL,NAMARC) + WRITE(KBOUT,1002)NAMARC + WRITE(IDEV5,1003)NAMARC + FILNAM = NAMARC + CALL OPENRS(IDEV1,NAMARC,IOK,LRECL,1) + IF(IOK.NE.0) GO TO 100 + CALL READW(IDEV3,JL,GEL,MAXGEL) + L=ABS(LNGTHG(JL)) + IF(LNGTHG(JL).LT.0)THEN + CALL SQREV(GEL,L) + CALL SQCOM(GEL,L) + END IF + CALL FMTDKN(IDEV1,GEL,L) + CLOSE(UNIT=IDEV1) + IF(JR.EQ.JL)GO TO 20 + CALL READN(IDEV4,JR,NAMARC) + WRITE(KBOUT,1002)NAMARC + WRITE(IDEV5,1003)NAMARC + CALL OPENRS(IDEV1,NAMARC,IOK,LRECL,1) + IF(IOK.NE.0) GO TO 100 + CALL READW(IDEV3,JR,GEL,MAXGEL) + L=ABS(LNGTHG(JR)) + IF(LNGTHG(JR).LT.0)THEN + CALL SQREV(GEL,L) + CALL SQCOM(GEL,L) + END IF + CALL FMTDKN(IDEV1,GEL,L) + CLOSE(UNIT=IDEV1) +20 CONTINUE + RETURN +100 CONTINUE + WRITE(KBOUT,*)'Error opening file for extracted gel reading' + RETURN + END + SUBROUTINE GETLN2(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LINCON,LLINO,IGELNO,IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),GELID + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER FILEH*(*) + EXTERNAL GELID + IERR = 1 + NCONTC = GELID(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LLINO, + +IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH,INFLAG) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.4) RETURN + IF(NCONTC.EQ.0) RETURN + IGELNO = NCONTC + IF(LNBR(NCONTC).NE.0) THEN + WRITE(KBOUT,1013)RELPG(NCONTC) +1013 FORMAT(' Position of this reading=',I6) +25 CONTINUE + NCONTC = LNBR(NCONTC) + IF(LNBR(NCONTC).NE.0) GO TO 25 + WRITE(KBOUT,1014)NCONTC +1014 FORMAT( ' Number of leftmost reading this contig=',I6) + END IF +30 CONTINUE + N = IDBSIZ - NCONTS + DO 20 J=N,IDBSIZ-1 + IF(LNBR(J).EQ.NCONTC) THEN + LINCON=J + GO TO 21 + END IF +20 CONTINUE + WRITE(KBOUT,9999) +9999 FORMAT(' No contig line for this gel! Fix the database') + RETURN +21 CONTINUE + LLINO = NCONTC + IERR = 0 + END + SUBROUTINE GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LINCON,LLINO,IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) + CALL GETLN2(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LINCON,LLINO,IGELNO,IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH) + END + SUBROUTINE GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LEFTMN,RIGHTM,LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER LREG,RREG,RIGHTM + CHARACTER FILEH*(*) +40 CONTINUE + MN = LEFTMN + MX = RIGHTM + LREG = MN + CALL GETINT(MN,MX,LREG, + +'Start position in contig', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + LREG = IVAL + MN = LREG + MX = RIGHTM + RREG = MX + CALL GETINT(MN,MX,RREG, + +'End position in contig', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + RREG = IVAL +C NOW FIND FIRST GEL THAT OVER LAPS +50 CONTINUE + X=RELPG(LLINO)+(ABS(LNGTHG(LLINO)))-1 + IF(X.GE.LREG)GO TO 60 +C NOT IN REGION + LLINO=RNBR(LLINO) + GO TO 50 +60 CONTINUE + RETURN + END + SUBROUTINE GLEVEL(T,YF,YT,Y0,YP1,YP2,YM1,YM2) + CHARACTER T + IF(T.EQ.'0') THEN + YF = Y0 + YT = Y0 + ELSE IF(T.EQ.'1') THEN + YF = Y0 + YT = YM1 + ELSE IF(T.EQ.'2') THEN + YF = Y0 + YT = YP1 + ELSE IF(T.EQ.'3') THEN + YF = YP1 + YT = YM1 + ELSE IF(T.EQ.'4') THEN + YF = YP2 + YT = YM2 + END IF + END + CHARACTER*1 FUNCTION GTCONC(COUNTS,IDM,CUT) + INTEGER IDM + INTEGER COUNTS(IDM) + CHARACTER CHARSU + EXTERNAL CHARSU +C 8-4-92 made this routine sum counts + GTCONC = '-' + ISUM = 0 + DO 5 I=1,IDM + ISUM = ISUM + COUNTS(I) + 5 CONTINUE + IF(ISUM.EQ.0.) RETURN + Y = ISUM + DO 10 I = 1,IDM - 1 + X = REAL(COUNTS(I))/Y + IF(X.GE.CUT) THEN + GTCONC = CHARSU(I) + RETURN + END IF +10 CONTINUE + END + SUBROUTINE HIGHLT(GELSAV,NAMSAV,NUMSAV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IDEV1,IDEV2, + +FILNAM,IOK) + CHARACTER LINEIN*119,CONSEN*100 + CHARACTER GELNO*4,GEL*100,GELSAV*100,GELNAM*10 + CHARACTER NAMSAV*10,NUMSAV*4 + CHARACTER FILNAM*(*),HELPF*(*) + DIMENSION GELSAV(50),NAMSAV(50),NUMSAV(50) + CHARACTER PLUS*4,MINUS*4 + EQUIVALENCE (LINEIN(2:2),GELNO),(LINEIN(8:8),GELNAM) + EQUIVALENCE (LINEIN(20:20),GEL) + EXTERNAL NOTIRL + CALL OPENF1(IDEV1,FILNAM,0,IOK,KBIN,KBOUT, + +'File containing contig display', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,1,IOK,KBIN,KBOUT, + +'File for problem display', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN +C +C FORMAT: +C +C12345678901234567890 10 20 30 ETC +C 12 GELNAM0000 CAGACGCGCGCGCGCGCGGATATAGTCTCTCCGCTCT +C 100 GELNAM0000 TGATACGCTCGCTCTCTCTCTCTCTCTCTTTC +C AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA +C +C 70 80 ETC +C 12 GELNAM0000 AAAAAAAAAAAAAAAAAAAAAAAAAAAA +C +C + LIN = 1 + CALL GTSTR('plus strand symbol',':',PLUS,LIN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + IF(LIN.EQ.0) PLUS = ':' + LIN = 1 + CALL GTSTR('minus strand symbol','.',MINUS,LIN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + IF(LIN.EQ.0) MINUS = '.' +C COUNT LINE NUMBERS + LINNO=0 +10 CONTINUE +C READ LINE OF NOS + READ(IDEV1,1003,END=100)LINEIN + LINNO=LINNO+1 +1003 FORMAT(A) +C WRITE IT OUT AGAIN + WRITE(IDEV2,1003)LINEIN +C ZERO GEL COUNT FOR THIS STRIP + IGEL=0 +20 CONTINUE +C +C READ A LINE, COULD BE 1 GEL, 2 CONSENSUS OR BLANK +C LINEIN=' ' + READ(IDEV1,1003,END=100)LINEIN + LINNO=LINNO+1 +C WHAT SORT OF LINE? ONLY A GEL WILL HAVE NON BLANK CHARS AT THE LEFT END + IF(LINEIN(2:5).NE.' ')THEN +C GEL LINE SO SAVE + IGEL=IGEL+1 + GELSAV(IGEL)=GEL + NAMSAV(IGEL)=GELNAM + NUMSAV(IGEL)=GELNO + GO TO 20 + END IF +C MUST BE CONSENSUS + CONSEN=GEL +C PROCESS THIS STRIP OF GELS (IGEL OF THEM) + DO 50 I=1,IGEL +C WHERE DOES DATA START AND END? + IFIRST=1 +40 CONTINUE + IF(GELSAV(I)(IFIRST:IFIRST).NE.' ')GO TO 45 + IFIRST=IFIRST+1 + IF(IFIRST.LE.100)GO TO 40 +C ERROR --- NO DATA FOUND + WRITE(KBOUT,1004)LINNO +1004 FORMAT(' Error on line',I6,' of file') + RETURN +45 CONTINUE +C NOW WHERE DOES IT END + ILAST=NOTIRL(GELSAV(I),100,' ') +C COMPARE WITH CONSENSUS + READ(NUMSAV(I),'(I4)',ERR=900)INTEG + IF(INTEG.GE.0)CALL IDTOD(CONSEN,GELSAV(I),IFIRST,ILAST,PLUS) + IF(INTEG.LT.0)CALL IDTOD(CONSEN,GELSAV(I),IFIRST,ILAST,MINUS) + WRITE(IDEV2,1008)NUMSAV(I),NAMSAV(I),GELSAV(I)(1:ILAST) +1008 FORMAT(' ',A,2X,A,2X,A) +50 CONTINUE + WRITE(IDEV2,1009)CONSEN +1009 FORMAT(' ',18X,A) +1006 FORMAT( ) +C READ A BLANK LINE + READ(IDEV1,1003,END=100)LINEIN + LINNO=LINNO+1 + WRITE(IDEV2,1003)LINEIN +C NO GO BACK FOR THE NEXT LINE OF NUMBERS + GO TO 10 +100 CONTINUE + WRITE(KBOUT,1005) +1005 FORMAT(' Finished') + RETURN +900 WRITE(KBOUT,*)'Error reading gel number' + END + SUBROUTINE IDPLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ, + +NCONTS,IX,IY,MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX, + +DBTDUX,DBTDUY,NCONT,IGEL,IS) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER CHNRP1 + EXTERNAL CWORLD,CHNRP1 + YMAX = ISYMAX + YMIN = 0. + XMIN = 0. + LENCON = 0 + DO 10 I = IDBSIZ-NCONTS,IDBSIZ-1 + LENCON = LENCON + RELPG(I) +10 CONTINUE + XMAX = LENCON + XX = CWORLD(IX,MARGL,MARGR,XMIN,XMAX) + YX = CWORLD(IY,MARGB,MARGT,YMIN,YMAX) + YINC = (YMAX-YMIN)/3. + Y = 0. + XF = XMIN + N = 0 + DO 20 I = IDBSIZ-NCONTS,IDBSIZ-1 + N = N + 1 + XT = XF + RELPG(I) + Y = Y + YINC + IF((XX.GT.XF).AND.(XX.LT.XT)) THEN + IS = NINT(((XX-XF)/(XT-XF)) * RELPG(I)) + JGEL = LNBR(I) + IGEL = CHNRP1(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,JGEL,IS) + NCONT = I + RETURN + END IF + XF = XT + IF(N.EQ.2) THEN + N = 0 + Y = 0. + END IF +20 CONTINUE + IGEL = 0 + NCONT = 0 + END + SUBROUTINE IDTOD(TOPLIN,GEL,IFIRST,ILAST,SYMBOL) + CHARACTER TOPLIN*100,GEL*100,SYMBOL*4 + DO 10 I=IFIRST,ILAST + IF(GEL(I:I).EQ.TOPLIN(I:I))GEL(I:I)=SYMBOL(1:1) +10 CONTINUE + END + INTEGER FUNCTION INDEXS(C,S) + PARAMETER (IDM = 29) + CHARACTER C + INTEGER POINTS(0:255),SCORES(IDM),IND(IDM),S + COMMON /SHOTC/POINTS + SAVE /SHOTC/ + SAVE SCORES,IND + DATA + +IND/1,2,3,4,1,2,3,4,1,2,3,4,1,2,3,4,6,6,6,6,6,6,1,2,3,4,5,5,6/ +C DATA DUP/'CTAG1234DVBHKLMNRY5678ctag*,-'/ +C changed 28-7-91 to give 10 to old zeroes and 100 to lowercase + DATA SCORES/ + +100,100,100,100, + +75,75,75,75, + +100,100,100,100, + +100,100,100,100, + +10,10,10,10,10,10, + +100,100,100,100,100,100,10/ + I = ICHAR(C) + I = POINTS(I) + S = SCORES(I) + INDEXS = IND(I) + END +C ROUTINES TO CONTROL CHARACTER LOOKUP FOR SHOTGUN SEQUENCING + SUBROUTINE INITS +C AUTHOR RODGER STADEN + INTEGER POINTS(0:255) + PARAMETER (IDM = 29) + CHARACTER DUP*29 + COMMON /SHOTC/POINTS + SAVE /SHOTC/ + DATA DUP/'CTAG1234DVBHKLMNRY5678ctag*,-'/ +C ICHAR RETURNS THE COLLATING SEQUENCE NUMBER +C I WANT 1-4 FOR ACGT +C acgt +C 1234 +C BDHV +C KLMN +C 5 FOR * +C 6 FOR 5678- AND ELSE +C THE ACTUAL VALUE RETURNED BY ICHAR IS NOT PORTABLE +C SO I NEED TO INITIALIZE POINTR SO THAT THE CORRECT +C ELEMENTS CONTAIN VALUES 1 - 6 +C + DO 30 I = 0,255 + POINTS(I) = IDM +30 CONTINUE + DO 35 I = 1,IDM + J = ICHAR(DUP(I:I)) + POINTS(J) = I +35 CONTINUE + END + FUNCTION INLIST(LIST,IDLIST,ITEM) +C AUTHOR: RODGER STADEN +C SENT LIST LIST, AND ITEM ITEM. IF IN LIST RETURNS ELEMENT NUMBER, ELSE 0 + INTEGER LIST(IDLIST) + INLIST=0 + DO 1 I=1,IDLIST + IF(LIST(I).NE.ITEM)GO TO 1 + INLIST=I + RETURN +1 CONTINUE + RETURN + END + SUBROUTINE IPLTC(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,STRAND, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,YMID,YINC,DEPTH,X,Y,KBOUT, + +IGEL,IOK) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG,STRAND,DEPTH + IOK = 1 + XMIN = LREG + XMAX = RREG + YMAX = ISYMAX + YMIN = 0. + YINCO2 = STRAND*YINC/2. + I = LGEL + IGEL = 0 +5 CONTINUE + IF(I.NE.0) THEN + IF((RELPG(I)+ABS(LNGTHG(I))-1).LT.LREG) THEN + I = RNBR(I) + GO TO 5 + END IF + END IF + N = 0 +10 CONTINUE + IF(I.NE.0)THEN + IF(RELPG(I).LE.RREG) THEN + IF(SIGN(1,LNGTHG(I)).EQ.STRAND) THEN + XF = MAX(RELPG(I),LREG) + XT = MIN(ABS(LNGTHG(I))+RELPG(I)-1,RREG) + N = N + 1 + IF(N.GT.DEPTH) N = 1 + YF = YMID + N * YINC + IF((X.GE.XF).AND.(X.LE.XT)) THEN + IGEL = I + IF((Y.GE.YF-YINCO2).AND.(Y.LE.YF+YINCO2)) THEN + IOK = 0 + RETURN + END IF + END IF + END IF + I = RNBR(I) + GO TO 10 + END IF + END IF + END + SUBROUTINE JOIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LNCONL,LLINOL,LNCONR,LLINOR,GEL,GEL2, + +IDBSIZ,IDEV,KBIN,KBOUT,IDEVR,IDEVW,IDEVN,LINLEN,PERCD, + +HELPS1,HELPE1,HELPS2,HELPE2,FILEH,IDEVH,MAXGEL,IDM) + CHARACTER FILEH*(*) + INTEGER HELPS1,HELPE1,HELPS2,HELPE2 +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),ANS + INTEGER LREG,RREG,X,RELX,RIGHTM + INTEGER LNGTHG(IDBSIZ),RNBR(IDBSIZ),LNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),GEL2(MAXGEL) + PARAMETER (MAXPRM = 32) + CHARACTER PRMPT(5)*(MAXPRM) +15 CONTINUE + WRITE(KBOUT,1001) +1001 FORMAT( + +' The joint is the position in the left contig that',/, + +' the first character of the right contig overlaps.') + MN = 1 + MX = RELPG(LNCONL) + RELX = 0 + CALL GETINT(MN,MX,RELX, + +'Position of join', + +IVAL,KBIN,KBOUT,HELPS1,HELPE1,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF(IVAL.EQ.0) RETURN + RELX = IVAL + I1=1 + I2=LINLEN + IF((RELPG(LNCONR)).LT.I2)I2=(RELPG(LNCONR)) + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR,GEL2, + +LLINOL,LNCONL,RELX,RELPG(LNCONL),GEL,I1,I2,3,LLINOR, + +IDBSIZ,IDEV,KBOUT,IDEVW,IDEVN,LINLEN,PERCD,MAXGEL,IDM) +30 CONTINUE + CALL BELL(1,KBOUT) +C CALL DBMENU(3,NOPT,HELPS1,HELPE1,FILEH,IDEVH, +C +KBIN,KBOUT) + PRMPT(1) = 'Complete join' + PRMPT(2) = 'Edit left contig' + PRMPT(3) = 'Display join' + PRMPT(4) = 'Edit right contig' + PRMPT(5) = 'Move join' + NOPT = 1 + CALL RADION('Join options', PRMPT, 4, NOPT, IHELPS, + + IHELPE, FILEH, IDEVH, KBIN, KBOUT) + IF(NOPT.LT.1)RETURN +100 CONTINUE + IF(NOPT.EQ.5)GO TO 15 + IF(NOPT.EQ.2)THEN +C EDIT LEFT CONTIG + IMAXL=RELX + IMAXR=MIN(RELPG(LNCONL),(RELX+RELPG(LNCONR)-1)) + CALL DBEDIT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,GEL2, + + LNCONL,IMAXL,IMAXR,IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW, + + HELPS2,HELPE2,FILEH,IDEVH,MAXGEL) + END IF +200 CONTINUE + IF(NOPT.EQ.4)THEN +C EDIT RIGHT CONTIG + IMAXL=1 + IMAXR=MIN((RELPG(LNCONL)-RELX+1),RELPG(LNCONR)) + CALL DBEDIT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,GEL2, + + LNCONR,IMAXL,IMAXR,IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW, + + HELPS2,HELPE2,FILEH,IDEVH,MAXGEL) + END IF +300 CONTINUE + IF(NOPT.EQ.3)THEN +C DISPLAY + LLINO=LLINOL + LEFTMN=RELX + RIGHTM=RELPG(LNCONL) + IF((RIGHTM-LEFTMN+1).GT.RELPG(LNCONR))RIGHTM= + + LEFTMN+RELPG(LNCONR)-1 + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + LEFTMN,RIGHTM,LREG,RREG, + + LNCONL,LLINO,IDBSIZ,KBIN,KBOUT, + + HELPS1,HELPE1,FILEH,IDEVH,IERR) + IF(IERR.NE.0) GO TO 30 + I1=(LREG-RELX)+1 + I2=(RREG-RELX)+1 + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR,GEL2,LLINO, + + LNCONL,LREG,RREG,GEL,I1,I2,3,LLINOR,IDBSIZ,IDEV,KBOUT, + + IDEVW,IDEVN,LINLEN,PERCD,MAXGEL,IDM) + END IF +40 CONTINUE + IF(NOPT.EQ.1)THEN +C COMPLETE JOIN + CALL YESNO(ANS,'Sure', + + HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) RETURN + IF(ANS.NE.0) GO TO 30 +C ADJUST ALL RELATIVE POSITIONS IN RIGHT CONTIG + N=LLINOR + RELPG(N)=RELX +50 CONTINUE + IF(RNBR(N).EQ.0)GO TO 60 + N=RNBR(N) + RELPG(N)=RELPG(N)+RELX-1 + GO TO 50 +60 CONTINUE +C +C FIX UP NEW GEL LINE FOR OLD LEFT OF RIGHT CONTIG + LNBR(LLINOR)=RNBR(LNCONL) +C FIX UP RIGHT GEL OF LEFT CONTIG + N=RNBR(LNCONL) + RNBR(N)=LLINOR + CALL MERGE(RELPG,LNGTHG,LNBR,RNBR,LNCONL,IDBSIZ) +C MERGE DOES NOT WRITE TO DISK + N=LNBR(LNCONL) +65 CONTINUE + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) + N=RNBR(N) + IF(N.NE.0)GO TO 65 +C CONTIG LINES + X=RELPG(LNCONR)+RELX-1 + IF(X.GT.RELPG(LNCONL))RELPG(LNCONL)=X + CALL WRITER(IDEVR,LNCONL,RELPG(LNCONL),LNGTHG(LNCONL), + +LNBR(LNCONL),RNBR(LNCONL)) +C NOW MOVE ALL DATA DOWN TO DELETE OLD RIGHT END + N=IDBSIZ-NCONTS + M=LNCONR-N + IF(M.EQ.0)GO TO 80 + K=LNCONR + J=LNCONR-1 + DO 70 I=1,M + RELPG(K)=RELPG(J) + LNGTHG(K)=LNGTHG(J) + LNBR(K)=LNBR(J) + RNBR(K)=RNBR(J) + CALL WRITER(IDEVR,K,RELPG(K),LNGTHG(K), + +LNBR(K),RNBR(K)) + K=K-1 + J=J-1 +70 CONTINUE +80 CONTINUE + NCONTS=NCONTS-1 + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + RETURN + END IF + GO TO 30 + END +C LINEUP +C +C TAKES 2 SEQS SET OF MATCHES AND PRODUCES LINED UP SEQS +C FINDS IF WE HAVE A LEFT OVERLAP +C RETURNS POSITION OF JOINT. THIS IS RELATIVE TO THE CONTIG +C FOR MOST MATCHES BUT I RELATIVE TO THE GEL FOR A LEFT OVERLAP + SUBROUTINE LINEUP(SEQG,SEQC,SEQG2,SEQC2,IDC,IDG,IDOUT, + 1MATG,MATC,MATL,IP,ITOTPC,ITOTPG,JOINT,ITYPE,KBOUT,MAXGEL,IFAIL) +C AUTHOR: RODGER STADEN + CHARACTER SEQG(IDG),SEQC(IDC),SEQG2(IDOUT),SEQC2(IDOUT),PAD + INTEGER MATG(IP),MATC(IP),MATL(IP) + SAVE PAD + DATA PAD/','/ + IFAIL=0 +C ZERO PADDING CHARS IN CONTIG (GEL DONE AT END BY DIFFERENCE +C IN INPUT AND OUTPUT LENGTHS) + ITOTPC=0 +C FILL OUTPUT WITH PADDING + DO 10 I=1,IDOUT + SEQG2(I)=PAD + SEQC2(I)=PAD +10 CONTINUE + NMTCH=0 +C SET INITIAL POINTERS TO OUTPUT +C CONSENSUS + IS1=1 +C GEL + IS2=1 +C FIND DISTANCE FROM LEFT MATCH IN GEL TO LEFT OF GEL + IG2=MATG(1)-1 + IF(IG2.EQ.0)THEN +C THE LEFT END OF THE GEL MATCHES SO THIS IS NOT A LEFT OVERLAP +C SET TYPE + ITYPE=-1 +C SET JOINT + JOINT=MATC(1) +C SKIP NEXT SECTION + GO TO 50 + END IF +C FIND DISTANCE FROM LEFT MATCH IN CONTIG TO LEFT OF CONTIG + IC2=MATC(1)-1 +C GET DISTANCE FROM FIRST MATCH IN CONTIG TO FIRST MATCH IN GEL. +C IF THIS DISTANCE <0 THEN WE HAVE A LEFT OVERLAP + IC1=IC2-IG2+1 + IF(IC1.GT.0)THEN +C THIS IS NOT A LEFT OVERLAP +C SET TYPE + ITYPE=-1 +C SET LEFT END + JOINT=IC1 +C COPY THE GEL UPTO THE FIRST MATCH, INTO THE OUTPUT ARRAY +C CHECK FOR OVERFLOW + IF(IG2.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQG(1),SEQG2(1),IG2) +C COPY THE CONTIG FOR THE SAME REGION + IF(IG2.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQC(IC1),SEQC2(1),IG2) + IS1=IS1+IG2 + IS2=IS2+IG2 + GO TO 50 + END IF +C MUST BE LEFT END OVERLAP +C SET TYPE + ITYPE=1 +C SET POSITION OF JOINT RELATIVE TO GEL + JOINT=ABS(IC1)+2 +C COPY OVER THE GEL UPTO THE JOINT +C CHECK FOR OVERFLOW + IF(IG2.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQG(1),SEQG2(1),IG2) + IS2=IS2+IG2 +C WE MAY ALSO HAVE MISMATCHING +C DATA AT THE JOIN SO DEAL WITH THAT NOW +C IF IC2 >0 THE LEFT END OF THE CONTIG MATCHES THE GEL BUT OTHERWISE +C WE HAVE SOME MISMATCHED DATA TO DEAL WITH - WE NEED TO TRANSFER +C THE MISMATCHED REGION OF THE CONTIG TO THE OUTPUT ARRAY + IF(IC2.GT.0)THEN + IF(IC2.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQC(1),SEQC2(1),IC2) + IS1=IS1+IC2 + END IF +C WHEN WE GET HERE WE HAVE SORTED OUT THE LEFT ENDS FOR LEFT OVERLAP +C AND MISMATCHED LEFT ENDS, WE NOW DEAL WITH THE REST OF THE SEQUENCE +C STARTING WITH THE FIRST BLOCK OF IDENTITY +C +C IG1 POSITION IN INPUT GEL +C IS2 POSITION IN OUTPUT GEL +C IC1 POSITION IN INPUT CONTIG +C IS1 POSITION IN OUTPUT CONTIG +C LG1 POSITION OF END OF CURRENT MATCH IN OUTPUT GEL +C LC1 POSITION OF END OF CURRENT MATCH IN OUTPUT CONTIG +C LG2 DISTANCE FROM CURRENT MATCH IN INPUT GEL TO NEXT MATCH +C LC2 DISTANCE FROM CURRENT MATCH IN INPUT CONTIG TO NEXT MATCH +C +50 CONTINUE +C POINT TO NEXT MATCH + NMTCH=NMTCH+1 +C COPY NEXT MATCH + IG1=MATG(NMTCH) + IC1=MATC(NMTCH) + L=MATL(NMTCH) +C CHECK FOR OVERFLOW + IF(IS2+L-1.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQG(IG1),SEQG2(IS2),L) +C CHECK FOR OVERFLOW + IF(IS1+L-1.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQC(IC1),SEQC2(IS1),L) +C POINT TO NEXT OUTPUT POSITIONS + IS1=IS1+L + IS2=IS2+L +C END OF CURRENT MATCH + LG1=IG1+L + LC1=IC1+L +C ANY MORE MATCHES + IF(NMTCH.EQ.IP)GO TO 500 + K=NMTCH+1 + LG2=MATG(K)-LG1 + LC2=MATC(K)-LC1 +C ANY DIFFERENCE IN LENGTH? IF SO WE HAVE TO PAD SO THEY BECOME THE SAME + L5=ABS(LG2-LC2) +C COUNT PADDING CHARS IN CONTIG + IF(LG2.GT.LC2)ITOTPC=ITOTPC+L5 +C IF DIFFERENCE INCREMENT SHORTER + IF(LG2.GT.LC2)IS1=IS1+L5 +C IF GEL NEEDS PADDING TRY TO PUT PADS NEXT TO DOUBLE CODES + IF(LC2.GT.LG2)CALL PADCOP(SEQG,SEQG2, + +LG1,MATG(K),L5,IS2,LG2,MAXGEL,IFAIL,KBOUT,SEQC,LC1) +C CHECK FOR OVERFLOW + IF(IFAIL.EQ.1)GO TO 700 +C NOW COPY MISSMATCHED REGION +C CHECK FOR OVERFLOW + IF(IS2+LG2-1.GT.MAXGEL)GO TO 700 + IF(LG2.GT.0)CALL SQCOPY(SEQG(LG1),SEQG2(IS2),LG2) +C CHECK FOR OVERFLOW + IF(IS1+LC2-1.GT.MAXGEL)GO TO 700 + IF(LC2.GT.0)CALL SQCOPY(SEQC(LC1),SEQC2(IS1),LC2) +C POINT TO NEXT OUTPUT POSITIONS + IS1=IS1+LC2 + IS2=IS2+LG2 +C GET NEXT MATCH + GO TO 50 +500 CONTINUE +C +C FINISH RIGHT ENDS +C ONLY COPY TO END OF GEL IN GEL AND TO THE SAME RELATIVE POSITION +C IN THE CONTIG FOR DISPLAY PURPOSES AND FOR COUNTING MISMATCH +C CURRENT ENDS AT LG1,LC1 +C HOW FAR TO END OF GEL? +C SET M + M=0 + L=IDG-LG1+1 + IF(L.LT.1)GO TO 600 +C CHECK FOR OVERFLOW + IF(IS2+L-1.GT.MAXGEL)GO TO 700 + CALL SQCOPY(SEQG(LG1),SEQG2(IS2),L) +C NEED TO COPY TO END OF GEL IN CONTIG FOR DISPLAY +C POINT TO POSN IN CONTIG LEVEL WITH END OF GEL + M=LC1+L-1 +C IS THIS OVER END OF CONTIG? + IF(M.GT.IDC)M=IDC +C NUMBER TO COPY + M=M-LC1+1 +C CHECK FOR OVERFLOW + IF(IS1+M-1.GT.MAXGEL)GO TO 700 + IF(M.GT.0)CALL SQCOPY(SEQC(LC1),SEQC2(IS1),M) +600 CONTINUE +C COUNT PADDING IN GEL + ITOTPG=IS2+L-1-IDG +C SET NEW LENGTHS FOR RETURN TO CALLING ROUTINE + IDOUT=IS1+M-1 + IDG=IS2+L-1 + IFAIL=0 + RETURN +700 CONTINUE + WRITE(KBOUT,1000) +1000 FORMAT(' Matching region too long for routine lineup,', + +' alignment aborted') + IFAIL=1 + RETURN + END + SUBROUTINE LSTCON(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LLINO, + +RREG,IDEV,IDEVN,NAMARC) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG + CHARACTER NAMARC*(*) + N = LLINO + WRITE(IDEV,1001) +10 CONTINUE + CALL READN(IDEVN,N,NAMARC) + WRITE(IDEV,1006)NAMARC,N,RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) + IF(RNBR(N).NE.0) THEN + N = RNBR(N) + IF(RELPG(N).LE.RREG) GO TO 10 + END IF +1001 FORMAT(' NAME NUMBER POSITION LENGTH NEIGHBOURS'/ + +' LEFT RIGHT') +1006 FORMAT( ' ',A,2X,I4,2X,I7,2X,I5,2X,I6,2X,I6) + END +C12345678901234567890 +C 710 720 730 740 750 +C -1 HINW.004 CGTCAGACGCACGCTGGAAAA + INTEGER FUNCTION LTYPE(LINE,LL,J1,J2,N,MAXDB,KBOUT) + CHARACTER LINE*(*),NUM*5,SPACE + EXTERNAL NOTRL,NOTLR + PARAMETER (SPACE= ' ') + J1 = NOTLR(LINE,LL,SPACE) + IF(J1.EQ.0) THEN +C BLANK LINE + LTYPE = 1 + RETURN + END IF + IF(J1.GT.20) THEN +C LINE OF NUMBERS + LTYPE = 2 + RETURN + END IF + IF(J1.GT.5) THEN +C CONSENSUS LINE + LTYPE = 3 + RETURN + END IF +C SHOULD BE A SEQUENCE LINE + J = INDEX(LINE(J1:),SPACE) + NUM = SPACE + NUM = LINE(J1:J1+J-2) + CALL RJST(NUM) +C N = IFROMS(NUM,5,KBOUT) + READ(NUM,'(I5)',ERR=10) N + IF(N.GT.MAXDB-2) GO TO 10 +C NUMBER ENDS AT J1+J-2 + J1 = J1 + J - 1 +C LOOK FOR BEGINNING OF NAME + J = NOTLR(LINE(J1:),LL-J1+1,SPACE) + N1 = J1 + J - 1 +C LOOK FOR END OF NAME + J = INDEX(LINE(N1:),SPACE) + N2 = N1 + J - 2 +C LOOK FOR BEGINNING OF SEQ + J = NOTLR(LINE(N2+1:),LL-N2,SPACE) + J1 = N2 + J + LTYPE = 4 +C LOOK FOR END OF SEQ + J2 = NOTRL(LINE,LL,SPACE) + IF(J2.GT.N2) RETURN +10 CONTINUE + LTYPE = 0 + END + INTEGER FUNCTION LWRAPS(I,J) + K = MOD(I,J) + IF(K.EQ.0) K = J + LWRAPS = K + END +C MERGE +C +C ROUTINE SENT CONTIG WHOSE GELS MAY BE OUT OF ORDER +C REORDERS GELS ON POSITION OF LEFT ENDS AND SETS LEFT +C GEL NUMBER FOR THE REORDERED CONTIG +C + SUBROUTINE MERGE(RELPG,LNGTHG,LNBR,RNBR,LINCON,IDBSIZ) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) +C +C START AT LEFT END + N=LNBR(LINCON) + GO TO 22 +21 CONTINUE +C SET POINTER TO NEXT GEL TO RIGHT IN LIST + N=NR + IF(I1.GT.0)N=I2 +22 CONTINUE +C SET POINTER TO NEXT GEL TO RIGHT + NR=RNBR(N) + IF(NR.EQ.0)GO TO 30 +C HAVENT REACHED END YET + I1=0 +23 CONTINUE +C ARE THESE 2 IN CORRECT ORDER IE N<=NR ? + IF(RELPG(N).LE.RELPG(NR))GO TO 21 +C NOT IN ORDER SO CHAIN LEFT UNTIL CORRECTLY POSITIONED +C THEN COME BACK TO THIS POINT AND CONTINUE +C IF FIRST MOVE SAVE POSITION + IF(I1.EQ.0)I2=N + I1=1 +C EXCHANGE NEIGHBOURS + M=RNBR(NR) + IF(M.NE.0)LNBR(M)=N + M=LNBR(N) + IF(M.NE.0)RNBR(M)=NR + RNBR(N)=RNBR(NR) + RNBR(NR)=N + LNBR(NR)=LNBR(N) + LNBR(N)=NR +C CHAIN BACK THRU LIST + N=LNBR(NR) + IF(N.EQ.0)GO TO 21 +C END NOT REACHED + GO TO 23 +30 CONTINUE +C ALL DONE POINTER AT RIGHT GEL + RNBR(LINCON)=N + RETURN + END + SUBROUTINE MINCOM(SEQ1,IDIM1,SEQ2,IDIM2,SAV1,SAV2,SAV3, + +IP,MINM,KBOUT) +C AUTHOR: RODGER STADEN +C + CHARACTER SEQ1(IDIM1),SEQ2(IDIM2) + INTEGER SAV1(IP),SAV2(IP),SAV3(IP) +C + IP1=IP + IP=0 +C +C SITUATION 1 + NT1=IDIM2-MINM + IES1=MINM-1 + ISS2=NT1+1 +C + DO 100 I=1,NT1 +C +C POINT TO FIRST CHAR-1 OF SEQ2 + ISS2=ISS2-1 +C POINT TO LAST CHAR SEQ1 + IES1=IES1+1 +C + N=0 +C + DO 200 J=1,IES1 +C STORE POINTER + JJ=J +C +C POINT TO SEQ2 + K=ISS2+J +C TEST FOR EQUALITY + IF(SEQ1(J).NE.SEQ2(K))GO TO 220 +C INCREMENT N + N=N+1 + GO TO 200 +220 CONTINUE +C TEST FOR SUFFICENTLY LARGE N + IF(N.GE.MINM)CALL SAVIT(N,J,K,IP,SAV1,SAV2,SAV3,IP1) +C TEST FOR OVERFLOW + IF(IP.GT.IP1)GO TO 5000 +C RESET N TO ZERO + N=0 +200 CONTINUE +C +C GOOD SCORE AT END? +C NEED TO INCREMENT POINTERS AS SAVIT EXPECTS TO BE POINTING AT NEXT +C MISMATCH + JJ=JJ+1 + KK=K+1 + IF(N.GE.MINM)CALL SAVIT(N,JJ,KK,IP,SAV1,SAV2,SAV3,IP1) +C TEST FOR OVERFLOW + IF(IP.GT.IP1)GO TO 5000 +C +100 CONTINUE +C +C +C SITUATION 2 + NT2=IDIM1-IDIM2+1 +C + DO 300 I=1,NT2 + N=0 +C + DO 400 J=1,IDIM2 +C SAVE POINTER + JJ=J +C +C SET POINTER TO SEQ1 + L=I+J-1 + IF(SEQ1(L).NE.SEQ2(J))GO TO 420 + N=N+1 + GO TO 400 +420 CONTINUE + IF(N.GE.MINM)CALL SAVIT(N,L,J,IP,SAV1,SAV2,SAV3,IP1) +C TEST FOR OVERFLOW + IF(IP.GT.IP1)GO TO 5000 + N=0 +400 CONTINUE + LL=L+1 + JJ=JJ+1 + IF(N.GE.MINM)CALL SAVIT(N,LL,JJ,IP,SAV1,SAV2,SAV3,IP1) +C TEST FOR OVERFLOW + IF(IP.GT.IP1)GO TO 5000 +300 CONTINUE +C +C +C SITUATION 3 + ISS1=IDIM1-IDIM2 +C + DO 500 I=1,NT1 +C +C POINT TO FIRST CHAR SEQ1 + K=ISS1+I + IES2=IDIM2-I + N=0 +C + DO 600 J=1,IES2 +C SAVE POINTER + JJ=J +C +C POINT TO SEQ1 + L=K+J + IF(SEQ1(L).NE.SEQ2(J))GO TO 620 + N=N+1 + GO TO 600 +620 CONTINUE + IF(N.GE.MINM)CALL SAVIT(N,L,J,IP,SAV1,SAV2,SAV3,IP1) +C TEST FOR OVERFLOW + IF(IP.GT.IP1)GO TO 5000 + N=0 +600 CONTINUE +C + LL=L+1 + JJ=JJ+1 + IF(N.GE.MINM)CALL SAVIT(N,LL,JJ,IP,SAV1,SAV2,SAV3,IP1) +C TEST FOR OVERFLOW + IF(IP.GT.IP1)GO TO 5000 +500 CONTINUE +C + RETURN +5000 CONTINUE +C OVERFLOW +C + WRITE(KBOUT,1000)IP1 +1000 FORMAT(/' TOO MANY MATCHES. LIMIT = ',I6) + RETURN + END + SUBROUTINE ML(PC,PG,L,N,J) + INTEGER PC(N),PG(N),L(N) + DO 10 I = J,N-1 + PC(I) = PC(I+1) + PG(I) = PG(I+1) + L(I) = L(I+1) +10 CONTINUE + END + SUBROUTINE MSTLKL(SEQ,IDIM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + CHARACTER CHARSU + EXTERNAL CHARSU,INDEXS + DO 100 I=1,IDIM + J = INDEXS(SEQ(I),K) + SEQ(I) = CHARSU(J) +100 CONTINUE + END + CHARACTER FUNCTION MUNOTP(IP) +C AUTHOR RODGER STADEN + CHARACTER PUP*26 + SAVE PUP + DATA PUP/'CSTPAGNDEQBZHRKMILVFYW-X? '/ + MUNOTP = '-' + IF((IP.GT.0).AND.(IP.LT.23))MUNOTP = PUP(IP:IP) + END + INTEGER FUNCTION NCDEP(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,IGEL, + +STRAND,RREG) + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG,STRAND + NCDEP = 0 + N = 0 + I = IGEL +10 CONTINUE + IF(I.NE.0) THEN + IF(RELPG(I).LE.RREG) THEN + IF(SIGN(1,LNGTHG(I)).EQ.STRAND) N = N + 1 + I = RNBR(I) + GO TO 10 + END IF + END IF + NCDEP = N + END + SUBROUTINE NEWDB(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,KBOUT, + +IDEVF,IDEVR,IDEVW,IDEVN,MAXDB,WGEL,MAXGEL,LINCON,KGEL,IOK) +C AUTHOR: RODGER STADEN + INTEGER RELPG(MAXDB),LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER LINEIN*80,GEL*62,WGEL(MAXGEL),GELNAM*10 + EQUIVALENCE (LINEIN(18:18),GEL) + EQUIVALENCE (LINEIN(8:8),GELNAM) + EXTERNAL LTYPE + LINLEN = 80 + LINENO=0 +C SET LENGTH OF CONTIG + LENCON=0 +C SET POSITION OF RIGHTMOST CHAR OF LAST BLOCK + ILASTR=0 +C SET NUMBER OF LAST GEL TO START SO WE CAN IDENTIFY RIGHT AND LEFT +C NEIGHBOURS + LASTS=0 +C NEED TO WHERE THE LEFT OF EACH BLOCK IS. ASSUME THE FIRST GEL IN A BLOCK +C IS LEVEL WITH THE LEFT EDGE FOR THAT BLOCK (IT SHOULD BE). SO NEED A FLAG +C (IGF)=0 IF FIRST =1 IF NOT, AND A POSITION (ILEFT) TO MARK THE LEFT EDGE +C NEED TO SET LEFT EDGE TO 1 SO LENGTH CALCULATION WORKS FOR FIRST BLOCK + ILEFT=1 +10 CONTINUE +C NEED TO ADD LENGTH OF LONGEST LINE IN LAST BLOCK TO CONSENSUS LENGTH + LENCON=LENCON+ILASTR-ILEFT+1 +C RESET POSITION OF RIGHTMOST CHAR OF LAST BLOCK + ILASTR=0 +C READ LINE OF NOS + READ(IDEVF,1003,END=100)LINEIN +1003 FORMAT(A) + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) +C WRITE(*,*)LINENO,LT + IF(LT.NE.2) GO TO 400 +C SET FLAG FOR NEW BLOCK + IGF=0 +20 CONTINUE +C +C READ A LINE, COULD BE A GEL OR A CONSENSUS + READ(IDEVF,1003,END=100)LINEIN + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) +C WRITE(*,*)LINENO,LT + IF(LT.EQ.0) GO TO 400 +C WHAT SORT OF LINE? + IF(LT.EQ.1) GO TO 400 + IF(LT.EQ.4) THEN + IFIRST = J1 - 17 + ILAST = J2 - 17 + NPOS=ABS(N) +C GEL LINE SO SAVE +C NEED TO FIND THE RIGHTMOST POSITION FOR EACH BLOCK + IF(ILAST.GT.ILASTR)ILASTR=ILAST +C IF FIRST GEL THIS BLOCK SAVE POSITION OF LEFT EDGE + IF(IGF.EQ.0)ILEFT=IFIRST +C SET FLAG FOR NOT FIRST GEL THIS BLOCK + IGF=1 +C START OF GEL? + IF(LNGTHG(NPOS).EQ.0)THEN +C YES SO SET RELPG + RELPG(NPOS)=LENCON+IFIRST-ILEFT+1 +C LNBR(NPOS)=LASTS +C SET RIGHT NEIGHBOUR OF LAST GEL TO START +C IF(LASTS.NE.0)RNBR(LASTS)=NPOS + LASTS=NPOS + CALL WRITEN(IDEVN,NPOS,GELNAM) + END IF +C COPY NEW SEQUENCE TO DISK + CALL READW(IDEVW,NPOS,WGEL,MAXGEL) + K1=ABS(LNGTHG(NPOS))+1 + K2=K1+ILAST-IFIRST + K11=IFIRST-1 +C WRITE(*,*)K1,K2,K11 + DO 25 I=K1,K2 + K11=K11+1 + WGEL(I)=GEL(K11:K11) +25 CONTINUE + CALL WRITEW(IDEVW,NPOS,WGEL,MAXGEL) +C UPDATE LENGTH + LNGTHG(NPOS)=SIGN(K2,N) +C GO BACK FOR NEXT LINE (CONSENSUS OR NEW GEL) + GO TO 20 + END IF +C SHOULD BE CONSENSUS, CHECK + IF(LT.NE.3) GO TO 400 +C READ WHAT SHOULD BE A BLANK LINE + READ(IDEVF,1003,END=100)LINEIN + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,IFIRST,ILAST,N,MAXDB,KBOUT) +C WRITE(*,*)LINENO,LT + IF(LT.NE.1) GO TO 400 + GO TO 10 +100 CONTINUE +C CHECK FOR MISSING BLANK AT END OF FILE + IF(LT.NE.1) LENCON=LENCON+ILASTR-ILEFT+1 + LNBR(LINCON) = KGEL + CALL MERGE(RELPG,LNGTHG,LNBR,RNBR,LINCON,IDBSIZ) +C ON INPUT LNBR(LINCON) MAY BE WRONG, BUT ON EXIT RNBR IS CORRECT + I = RNBR(LINCON) +300 CONTINUE +C WRITE(*,*)I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + J = I + I = LNBR(I) + IF(I.NE.0) GO TO 300 +C WRITE CONTIG LINE + I = LINCON + RELPG(I) = LENCON + LNGTHG(I) = 0 + LNBR(I) = J +C WRITE(*,*)I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + WRITE(KBOUT,1005) +1005 FORMAT(' Finished, no errors found') +C + IOK = 0 + RETURN +400 CONTINUE +C ERROR DETECTED, REPORT LINE NUMBER AND STOP + WRITE(KBOUT,5002)LINENO +5002 FORMAT(' Error in line ',I6,' of file. Processing stopped', + +/,' but your database may be corrupted. Revert to a copy') + IOK = 1 + RETURN + END + SUBROUTINE PADCOP(SEQG,SEQG2,LG1,MG,L5,IS2,LG2,MAXGEL,IFAIL, + +KBOUT,SEQC,IC1) +C AUTHOR: RODGER STADEN + PARAMETER (NDUBL = 4) + CHARACTER SEQG(MAXGEL),SEQG2(MAXGEL),DUBBL(NDUBL),SEQC(MAXGEL) + SAVE DUBBL + DATA DUBBL/'D','B','V','H'/ + JC1 = IC1 +C Make seqg2 from seqg placing L5 padding chars before position MG +C which is the start of the next block of identity. Try to put the +C padding either in line with consensus pads, or next to double +C codes. The positions in seqg are LG1 to MG-1. seqg2 needs to be long +C enough to be extended from IS2 to IS2 + L5 -1 + MGM1-LG1 +1 +C ie we add L5 pads, plus the chars between and including LG1 and MGM1 + IDONE=0 +C POINT TO END OF MISMATCH + MGM1=MG-1 +C MAY BE NO CHARS TO COPY + IF(MGM1.LT.LG1)GO TO 111 +C Next check added 26-2-91 + MAXREQ = IS2 + L5 - 1 + MGM1 - LG1 + 1 + IF((MGM1.GT.MAXGEL).OR.(MAXREQ.GT.MAXGEL)) THEN + WRITE(KBOUT,1000) +1000 FORMAT(' Matching region too large for routine padcop,', + + ' alignment aborted') + IFAIL=1 + RETURN + END IF + DO 110 J=LG1,MGM1 + IF(IDONE.LT.L5) THEN + IF((JC1.GT.0).AND.(JC1.LT.MAXGEL)) THEN + IF(SEQC(JC1).EQ.'*') THEN + IS2 = IS2 + 1 + JC1 = JC1 + 1 + IDONE = IDONE + 1 + GO TO 109 + END IF + END IF + DO 108 M=1,NDUBL + IF(SEQG(J).EQ.DUBBL(M)) THEN + IS2 = IS2 + 1 + JC1 = JC1 + 1 + IDONE = IDONE + 1 + GO TO 109 + END IF +108 CONTINUE +109 CONTINUE + END IF + SEQG2(IS2) = SEQG(J) + IS2 = IS2 + 1 + JC1 = JC1 + 1 +110 CONTINUE +111 CONTINUE +C ALL CHARS COPIED. ENOUGH PADDING? + IF(IDONE.LT.L5)IS2=IS2+L5-IDONE +C IS2 SHOULD NOW BE POINTING AT NEXT CHAR +C ZERO LG2 TO SHOW CALLING ROUTINE COPYING DONE + LG2=0 + IFAIL=0 + END + SUBROUTINE PADRUN(GEL,IFIRST,ILAST,RUN) + CHARACTER PAD,GEL*50,RUN*50 + SAVE PAD + DATA PAD/'*'/ +C FIND PADS IN THIS GEL + DO 20 I = IFIRST,ILAST + IF(GEL(I:I).EQ.PAD) THEN +C IS IT AT LEAST THE SECOND IN A RUN + IF(RUN(I:I).EQ.PAD) THEN +C FIND THE LEFT END OF THE RUN + JJ = I +1 CONTINUE + IF(JJ.GT.1)THEN + JJ = JJ - 1 + IF(RUN(JJ:JJ).EQ.PAD)GO TO 1 + END IF +C FOUND LEFT END OF RUN AT JJ, SO MOVE PAD IF NOT ALREADY A PAD +5 CONTINUE + IF(GEL(JJ:JJ).NE.PAD)THEN +C EXCHANGE ELEMENTS + GEL(I:I) = GEL(JJ:JJ) + GEL(JJ:JJ) = PAD + ELSE +C ALREADY A PAD, SO TRY NEXT TO RIGHT + JJ = JJ + 1 + IF(JJ.LT.I) GO TO 5 + END IF + END IF + END IF +20 CONTINUE + END + SUBROUTINE PCON1(CHAR,CHRSUM) +C AUTHOR RODGER STADEN +C PART OF PROTEIN 'CONSENSUS' CALCULATION + CHARACTER CHAR + INTEGER CHRSUM + INTEGER CTONUM + EXTERNAL CTONUM + K = CTONUM(CHAR) + IF(K.NE.26)THEN + IF(CHRSUM.EQ.0)THEN + CHRSUM = K + ELSE + IF(K.NE.CHRSUM)CHRSUM = -1 + END IF + END IF + END + SUBROUTINE PLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LINCON,IGEL, + +NCONTS,MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + YMIN = 0. + YMAX = ISYMAX + XMIN = 0. + LENCON = 0 + DO 10 I = IDBSIZ-NCONTS,IDBSIZ-1 + LENCON = LENCON + RELPG(I) +10 CONTINUE + XMAX = LENCON + YINC = (YMAX-YMIN)/3. + Y = 0. + XF = XMIN + N = 0 + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + DO 20 I = IDBSIZ-NCONTS,IDBSIZ-1 + N = N + 1 + XT = XF + RELPG(I) + Y = Y + YINC + CALL LINE(XF,XT,Y,Y,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + IF((IGEL.NE.0).AND.(I.EQ.LINCON)) THEN + XZ = XF + RELPG(IGEL) + ABS(LNGTHG(IGEL))/2 + CALL LINE(XZ,XZ,YMAX,YMIN,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + XF = XT + IF(N.EQ.2) THEN + N = 0 + Y = 0. + END IF +20 CONTINUE + CALL VT100M + END + SUBROUTINE PLTC(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,STRAND, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,YMID,YINC,DEPTH) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG,STRAND,DEPTH + XMIN = LREG + XMAX = RREG + YMAX = ISYMAX + YMIN = 0. + I = LGEL +5 CONTINUE + IF(I.NE.0) THEN + IF((RELPG(I)+ABS(LNGTHG(I))-1).LT.LREG) THEN + I = RNBR(I) + GO TO 5 + END IF + END IF + N = 0 +10 CONTINUE + IF(I.NE.0)THEN + IF(RELPG(I).LE.RREG) THEN + IF(SIGN(1,LNGTHG(I)).EQ.STRAND) THEN + XF = MAX(RELPG(I),LREG) + XT = MIN(ABS(LNGTHG(I))+RELPG(I)-1,RREG) + N = N + 1 + IF(N.GT.DEPTH) N = 1 + YF = YMID + N * YINC + CALL LINE(XF,XT,YF,YF,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + I = RNBR(I) + GO TO 10 + END IF + END IF + END + SUBROUTINE PLTCON(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ, + +MARGL,MARGR,MARGB, + +MARGT,ISXMAX,ISYMAX,LGEL,LREG,RREG,DEPTHP,DEPTHM) + INTEGER DEPTHP,DEPTHM + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER RREG,STRAND +C have window size margt starting at margb +C depths depthp, depthm + YMAX = ISYMAX + YMIN = 0. + XMIN = LREG + XMAX = RREG + RINC = YMAX / (DEPTHP + DEPTHM + 2) + RMID =(DEPTHM+1) * RINC + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(XMIN,XMAX,RMID,RMID,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL TEXT(XMIN,RMID,'*',1,ISIZE,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL TEXT(XMAX,RMID,'*',1,ISIZE,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + STRAND = 1 + YINC = RINC * STRAND + CALL PLTC(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,STRAND, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,RMID,YINC,DEPTHP) + STRAND = -1 + YINC = RINC * STRAND + CALL PLTC(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,LGEL,LREG,RREG,STRAND, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,RMID,YINC,DEPTHM) + CALL VT100M + END + SUBROUTINE PLTQ(SEQ,IDIM2, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CHARACTER SEQ(IDIM2),T + PARAMETER (Y0 = 0., + + YP1 = 1., + + YP2 = 2., + + YM1 = -1., + + YM2 = -2.) + XMIN = 0. + XMAX = IDIM2 + YMIN = YM2 + YMAX = YP2 + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(XIN,XMAX,Y0,Y0,XMAX,XMIN,YMAX,YMIN, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + I = 1 +10 CONTINUE + XF = I + T = SEQ(I) +20 CONTINUE + IF(SEQ(I).NE.T) THEN + CALL GLEVEL(T,YF,YT,Y0,YP1,YP2,YM1,YM2) + XT = I - 1 + CALL LINE(XF,XF,YF,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(XF,XT,YT,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(XT,XT,YF,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + GO TO 10 + END IF + I = I + 1 + IF(I.LT.IDIM2) GO TO 20 + CALL GLEVEL(T,YF,YT,Y0,YP1,YP2,YM1,YM2) + XT = I + CALL LINE(XF,XF,YF,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(XF,XT,YT,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL LINE(XT,XT,YF,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL VT100M + END + SUBROUTINE POSTC(GELNOS,GELSTR,GELEND,LINEIN,IERR, + +KBOUT,IDEVF,MAXDB,GELNO,LINNO,MAXLIN,RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,KGEL) +C AUTHOR: RODGER STADEN + CHARACTER LINEIN*(*) + INTEGER GELNOS(MAXDB),GELSTR(MAXDB),GELEND(MAXDB) + INTEGER GELNO(MAXLIN,2),LINNO(MAXLIN,2),IGEL(2) + INTEGER LNBR(MAXDB),RNBR(MAXDB),RELPG(MAXDB),LNGTHG(MAXDB) + EXTERNAL LTYPE +C GELNOS === GEL NUMBERS (UNIQUE) +C GELSTR === GEL START LINES +C GELEND === GEL END LINES +C LINNO === GEL LINE NUMBERS PER STRIP +C IGEL === NUMBER OF GELS PER STRIP +C LINENO === CURRENT LINE NUMBER +C IUNIQ IS NUMBER OF GELS FOUND +C +C PROBLEM IS TO FIND IF THERE ARE LINES OF WORKING VERSIONS MISSING +C WE PROCESS THE DATA IN PAIRS OF BLOCKS OR STRIPS (A BLOCK OR STRIP IS ONE 50 +C CHARACTER WIDTH OF THE CONTIG). IF WE FIND ALL THE GELS THAT APPEAR +C IN STRIP B THEN IF THEY WEREN'T PRESENT IN STRIP A THEY MUST START IN +C STRIP B, IF THEY DONT APPEAR IN STRIP C THEY END IN STRIP B. +C STRATEGY IS TO READ THRU FILE AND REMEMBER WHICH GELS APPEAR IN EACH +C STRIP. BY COMPARING THEM WITH THE LAST STRIP WE CAN KNOW WHICH HAVE ENDED +C AND WHICH HAVE STARTED. HENCE WE STORE A LIST OF GELS AND THEIR START AND +C END LINE NUMBERS. USING THIS WE CAN SEE IF THE LIST IS UNIQUE +C SUB CLIST IS SENT THE CURRENT TWO LISTS OF GEL NUMBERS AND FINDS WHICH HAVE +C STARTED AND ENDED AND BUILDS UP A LIST OF UNIQUE GELS AND THEIR START +C AND END LINE NUMBERS +C + LINLEN = 80 + IERR=0 + JSTRIP=1 + LINENO=0 + ISTRIP=1 + IUNIQ=0 +10 CONTINUE +C READ WHAT SHOULD BE A LINE OF NOS + READ(IDEVF,1003,END=100)LINEIN +1003 FORMAT(A) + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) +C IS IT A LINE OF NOS? + IF(LT.NE.2) GO TO 400 +C ZERO GEL COUNT FOR THIS STRIP + IGEL(ISTRIP)=0 +20 CONTINUE +C READ A LINE, COULD BE 1 GEL, 2 CONSENSUS OR BLANK + READ(IDEVF,1003,END=100)LINEIN + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) + IF(LT.EQ.0) GO TO 400 + IF(LT.EQ.1) GO TO 400 + IF(LT.EQ.4) THEN +C GEL LINE SO SAVE +C INCREMENT COUNT TO NUMBER OF GEL + IGEL(ISTRIP)=IGEL(ISTRIP)+1 + GELNO(IGEL(ISTRIP),ISTRIP)=N + LINNO(IGEL(ISTRIP),ISTRIP)=LINENO +C BO BACK FOR NEXT LINE (CONSENSUS OR NEW GEL) + GO TO 20 + END IF +C SHOULD THEN BE A CONSENSUS + IF(LT.NE.3) GO TO 400 +C MUST BE CONSENSUS SO PROCESS THIS STRIP +C PROCESS THIS STRIP OF GELS (IGEL OF THEM) +C WHICH GELS ARE NEW? AND WHICH HAVE ENDED +C IF FIRST EVER STRIP DONT DO THIS + IF(JSTRIP.EQ.1)THEN + DO 6 I=1,IGEL(1) + GELNOS(I)=GELNO(I,1) + GELSTR(I)=LINNO(I,1) +6 CONTINUE + JSTRIP=JSTRIP+1 + ISTRIP=2 + IUNIQ=IGEL(1) +C READ WHAT SHOULD BE A BLANK LINE + READ(IDEVF,1003,END=100)LINEIN + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) + IF(LT.EQ.0) GO TO 400 + IF(LT.NE.1)GO TO 400 + GO TO 10 + END IF +C ORER OF ARGUMENTS DEPENDS ON ISTRIP + IF(ISTRIP.EQ.2)THEN + CALL CLIST(GELNO(1,1),LINNO(1,1),IGEL(1), + + GELNO(1,2),LINNO(1,2),IGEL(2), + + GELNOS,GELSTR,GELEND,MAXDB,IUNIQ,KBOUT,IERR) + IF(IERR.NE.0) GO TO 400 + ISTRIP=1 +C READ WHAT SHOULD BE A BLANK LINE + READ(IDEVF,1003,END=100)LINEIN + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) + IF(LT.EQ.0) GO TO 400 + IF(LT.NE.1)GO TO 400 + GO TO 10 + END IF + IF(ISTRIP.EQ.1)THEN + CALL CLIST(GELNO(1,2),LINNO(1,2),IGEL(2), + + GELNO(1,1),LINNO(1,1),IGEL(1), + + GELNOS,GELSTR,GELEND,MAXDB,IUNIQ,KBOUT,IERR) + IF(IERR.NE.0) GO TO 400 + ISTRIP=2 + READ(IDEVF,1003,END=100)LINEIN + LINENO=LINENO+1 + LT = LTYPE(LINEIN,LINLEN,J1,J2,N,MAXDB,KBOUT) + IF(LT.EQ.0) GO TO 400 + IF(LT.NE.1)GO TO 400 + GO TO 10 + END IF +100 CONTINUE +C MAY BE SOME LEFT IN CURRENT STRIP THAT HAVE NOT BEEN ENDED +C BUT ISTRIP WILL JUST HAVE BEEN CHANGED SO CHANGE IT BACK + IF(ISTRIP.EQ.1)THEN + ISTRIP=2 + GO TO 101 + END IF + ISTRIP=1 +101 CONTINUE + DO 110 I=1,IGEL(ISTRIP) + MATCH=INLIST(GELNOS,IUNIQ,GELNO(I,ISTRIP)) + IF(MATCH.NE.0)THEN + GELEND(MATCH)=LINNO(I,ISTRIP) + GO TO 110 + END IF +C ERROR + WRITE(KBOUT,1300)GELNO(I,ISTRIP) +1300 FORMAT( ' Error: gel number ',I5, + + ' expected but not found in list') + IERR = 1 + GO TO 400 +110 CONTINUE +C AS A CHECK: ARE THE UNIQUE GELS UNIQUE? + DO 120 I=2,IUNIQ + KD=IUNIQ-I+1 + IN=INLIST(GELNOS(I),KD,GELNOS(I-1)) + IF(IN.EQ.0)GO TO 120 + IERR=1 + WRITE(KBOUT,1006)GELNOS(I-1),GELSTR(I-1),GELSTR(IN) +1006 FORMAT(' Error: gel',I6,' has a line of data missing!'/ + + ' and appears to start on lines',I6,' and',I6) + GO TO 400 +120 CONTINUE + IF(IERR.EQ.0)WRITE(KBOUT,1005) +1005 FORMAT(' Successfully checked line order and missing lines') +C WRITE(*,*)(GELNOS(K),K=1,IUNIQ) + KGEL1 = KGEL + KGEL = ABS(GELNOS(1)) +C CHECK THAT THE LEFT GEL NUMBER IS UNCHANGED + IF(KGEL1.NE.KGEL) THEN + WRITE(KBOUT,1001) +1001 FORMAT(' Left gel number of edited contig does not agree',/, + + ' with original, processing aborted') + IERR = 1 + RETURN + END IF + LNBR(KGEL) = 0 + RELPG(KGEL) = 0 + LNGTHG(KGEL) = 0 + DO 200 I = 2,IUNIQ + J1 = ABS(GELNOS(I-1)) + J2 = ABS(GELNOS(I)) + RNBR(J1) = J2 + LNBR(J2) = J1 + RELPG(J2) = 0 + LNGTHG(J2) = 0 +200 CONTINUE + RNBR(J2) = 0 + IERR = 0 + RETURN +400 CONTINUE +C ERROR + WRITE(KBOUT,1007)LINENO +1007 FORMAT(' Error in line',I6,', of file: lines out of order'/, + +' processing stopped') + IERR=1 + RETURN + END + SUBROUTINE READN(IDEVN,N,NAME) + CHARACTER NAME*(*) + READ(IDEVN,REC=N)NAME + RETURN + END + SUBROUTINE READR(IDEVR,N,RELPG,LNGTHG,LNBR,RNBR) + INTEGER RELPG,RNBR + READ(IDEVR,REC=N)RELPG,LNGTHG,LNBR,RNBR + RETURN + END + SUBROUTINE READW(IDEVW,N,GEL,MAXGEL) + CHARACTER GEL(MAXGEL) + READ(IDEVW,REC=N)GEL + RETURN + END + SUBROUTINE REMOVL(MATC,MATG,MATL,IP) +C AUTHOR: RODGER STADEN + INTEGER MATC(IP),MATG(IP),MATL(IP) +C +C SET POINTER TO FIRST MATCH + NMTCH=0 +10 CONTINUE +C POINT TO NEXT MATCH + NMTCH=NMTCH+1 +C SORT MATCHES ON LENGTH + IPP=IP-NMTCH+1 + CALL BUBBL3(MATL(NMTCH),MATG(NMTCH),MATC(NMTCH),IPP) +C LOOK FOR END OF POSITIVES + DO 20 I=NMTCH,IP + J=I +20 IF(MATL(I).LT.1)GO TO 30 + J=J+1 +30 CONTINUE + IP=J-1 +C END OF POSITIVES AT IP + IF(NMTCH.GE.IP)RETURN + K1=MATC(NMTCH) + K2=K1+MATL(NMTCH)-1 + K3=MATG(NMTCH) + K4=K3+MATL(NMTCH)-1 +C POINT TO FIRST MATCH TO TEST + K6=NMTCH+1 + DO 200 I=K6,IP +C DO CONSENSUS FIRST +C OVERLAP? + IF(MATC(I).GT.K2)GO TO 100 + K5=MATC(I)+MATL(I)-1 + IF(K5.LT.K1)GO TO 100 +C DOES OVERLAP +C WHICH END + IF(K5.LE.K2)GO TO 80 +C LENGTH TO REDUCE MATCH BY IS IDELT + IDELT=K2-MATC(I)+1 +C NEW LENGTH + MATL(I)=MATL(I)-IDELT +C MOVE LEFT ENDS + MATC(I)=MATC(I)+IDELT + MATG(I)=MATG(I)+IDELT + GO TO 100 +80 CONTINUE +C LENGTH + MATL(I)=K1-MATC(I) +100 CONTINUE +C NOW LOOK FOR OVERLAPS WITH GEL +C OVERLAP? + IF(MATG(I).GT.K4)GO TO 200 + K5=MATG(I)+MATL(I)-1 + IF(K5.LT.K3)GO TO 200 +C DOES OVERLAP +C WHICH END? + IF(K5.LE.K4)GO TO 180 +C LENGTH TO REDUCE MATCH BY IS IDELT + IDELT=K4-MATG(I)+1 +C NEW LENGTH + MATL(I)=MATL(I)-IDELT +C MOVE LEFT ENDS + MATC(I)=MATC(I)+IDELT + MATG(I)=MATG(I)+IDELT + GO TO 200 +180 CONTINUE +C LENGTH + MATL(I)=K3-MATG(I) +200 CONTINUE + GO TO 10 + END +C SAVIT +C + SUBROUTINE SAVIT(N,J,K,IP,S1,S2,S3,IP1) +C AUTHOR: RODGER STADEN + INTEGER S1(IP1),S2(IP1),S3(IP1) +C + IP=IP+1 +C TEST FOR OVERFLOW + IF(IP.GT.IP1)RETURN + S1(IP)=N + S2(IP)=J-N + S3(IP)=K-N +C + RETURN + END + SUBROUTINE SCRENR(GEL,MAXGEL,STRING,NAME,FILNAM, + +IDEV1,IDEV2,IDEV3,IDEV4,IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER NAME*(*),FILNAM*(*),HELPF*(*) + CHARACTER GEL(MAXGEL),STRING(60) + CALL YESNO(INF,'Use file of file names', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(INF.LT.0) RETURN + IF(INF.EQ.0) THEN + FILNAM = ' ' + CALL OPENF1(IDEV1,FILNAM,0,IOK,KBIN,KBOUT, + + 'File of gel reading names', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,1,IOK,KBIN,KBOUT, + + 'File for names of sequences that pass', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + END IF + FILNAM = ' ' + CALL OPENF1(IDEV3,FILNAM,0,IOK,KBIN,KBOUT, + +'File name of recognition sequences', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN +C + JGEL = 0 + IGEL = 0 +1 CONTINUE + IF(INF.EQ.1) THEN +31 CONTINUE + MN = 0 + CALL GTSTR('Gel reading name',' ',NAME,MN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.3) RETURN + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 31 + END IF + ELSE + READ(IDEV1,1002,END=100)NAME + END IF +1002 FORMAT(A) + JGEL = JGEL + 1 + WRITE(IDEV,*)'Processing', JGEL,' in batch' + WRITE(IDEV,1003)NAME +1003 FORMAT(' Gel reading name ',A) + IDIMG=MAXGEL + CALL OPENRS(IDEV4,NAME,IOK,LRECL,2) + IF(IOK.NE.0)THEN + IF(INF.EQ.1) RETURN + WRITE(KBOUT,*)' Error opening gel reading file' + GO TO 1 + END IF + CALL ARRFIM(IDEV4,GEL,IDIMG,KBOUT) + CLOSE(UNIT=IDEV4) +2 CONTINUE + IF(IDIMG.LT.1)THEN + WRITE(KBOUT,*)' Gel reading too short to compare' + GO TO 1 + END IF + CALL MSTLKL(GEL,IDIMG) +3 CONTINUE + READ(IDEV3,1005,END=6)STRING +1005 FORMAT(60A1) +C FIND LENGTH OF STRING ASSUMING NO SPACES + DO 4 I=1,60 + II=I + IF(STRING(I).EQ.' ')GO TO 5 +4 CONTINUE +5 CONTINUE + II=II-1 + IF(II.GT.0)CALL FIND(GEL,IDIMG,STRING,II,JMATCH) + IF(JMATCH.EQ.0)GO TO 3 +C A MATCH + WRITE(IDEV,1007)JMATCH,(STRING(K),K=1,II) +1007 FORMAT(' Match at',I6,' with ',60A1) + REWIND IDEV3 + GO TO 1 +C NO MATCH SO SAVE +6 CONTINUE + WRITE(IDEV2,1002)NAME + IGEL = IGEL + 1 + REWIND IDEV3 + GO TO 1 +100 CONTINUE + WRITE(KBOUT,*)'Batch finished' + WRITE(KBOUT,*)JGEL,' compared and ',IGEL,' passed' + RETURN + END + SUBROUTINE SCRENV(MAXGEL, + +WORDP,WORDN,LPOWRC,POSNS,GELN, + +SEQ,MAXSEQ,GEL,GELCOP,MATCH, + +LENGTH, + +SAVPS,SAVPG,SAVL,MAXMAT,CENDS,NENDS,MAXCON,CONST, + +KBIN,KBOUT,IDEV1,IDEV2,IDEV3,IDEV4,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,FILNAM,NAME,IOK) + INTEGER POSNS(MAXSEQ),GELN(MAXGEL),WORDP(LPOWRC),SAVPS(MAXMAT) + INTEGER SAVPG(MAXMAT),SAVL(MAXMAT) + INTEGER WORDN(LPOWRC) + CHARACTER FILNAM*(*),NAME*(*),HELPF*(*) + CHARACTER GELCOP(MAXGEL) + INTEGER CENDS(MAXCON) + INTEGER NENDS(MAXCON) + INTEGER CONST(LENGTH) + CHARACTER SEQ(MAXSEQ),GEL(MAXGEL),MATCH(MAXGEL) + JGEL = 0 + IGELS = 0 + CALL YESNO(INF,'Use file of file names', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(INF.LT.0) RETURN + IF(INF.EQ.0) THEN + FILNAM = ' ' + CALL OPENF1(IDEV1,FILNAM,0,IOK,KBIN,KBOUT, + + 'File of gel reading names', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,1,IOK,KBIN,KBOUT, + + 'File for names of gel readings that pass', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + END IF + FILNAM = ' ' + CALL OPENF1(IDEV4,FILNAM,0,IOK,KBIN,KBOUT, + +'File name of vector sequence', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + IDIM = MAXSEQ + CALL ARRFIM(IDEV4,SEQ,IDIM,KBOUT) + CLOSE(UNIT=IDEV4) + MN = LENGTH*2 + MX = 50 + MINMAT = MAX(15,MN) + CALL GETINT(MN,MX,MINMAT, + +'Minimum initial match', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINMAT = IVAL + IDCEND=MAXCON + CALL FNDCON(SEQ,IDIM,CENDS,NENDS,IDCEND,MAXCON,KBOUT) +C IS THE VECTOR SEQUENCE IN THE CORRECT FORMAT WITH A TITLE AT THE FRONT? + IF(IDCEND.EQ.0)THEN + CENDS(1) = -19 + NENDS(1) = 1 + CENDS(2) = IDIM + 1 + IDCEND = 1 + END IF +C WRITE(KBOUT,9999) +C9999 FORMAT(' VECTOR SEQUENCE REQUIRES A TITLE EG ', +C 1' <---M13MP7.001----->') +C RETURN +C END IF + CALL BUSY(KBOUT) + CALL ENCO(SEQ,IDIM,POSNS,CONST,LENGTH) + CALL ENCONA(POSNS,IDIM,WORDP,WORDN,LPOWRC,LENGTH) +C +1 CONTINUE + IF(INF.EQ.1) THEN +3 CONTINUE + MN = 0 + CALL GTSTR('Gel reading name',' ',NAME,MN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.3) RETURN + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 3 + END IF + ELSE + READ(IDEV1,1002,END=100)NAME + END IF + JGEL = JGEL + 1 + WRITE(IDEV,*)'Processing',JGEL,' in batch' +1002 FORMAT(A) + WRITE(IDEV,1003)NAME +1003 FORMAT(' Gel reading name ',A) + IDIMG=MAXGEL + CALL OPENRS(IDEV3,NAME,IOK,LRECL,2) + IF(IOK.NE.0)THEN + IF(INF.EQ.1) RETURN + WRITE(IDEV,*)' Gel reading file not found' + GO TO 1 + END IF + CALL ARRFIM(IDEV3,GEL,IDIMG,KBOUT) + CLOSE(UNIT=IDEV3) +C LONG ENOUGH ? + IF(IDIMG.LT.MINMAT)THEN + WRITE(IDEV,*)' Gel reading too short to compare' + GO TO 1 + END IF + CALL SQCOPY(GEL,GELCOP,IDIMG) + ISTRAN=1 + IMATCH=0 +2 CONTINUE + CALL BUSY(KBOUT) + CALL MSTLKL(GEL,IDIMG) + CALL ENCO(GEL,IDIMG,GELN,CONST,LENGTH) + WRITE(IDEV,1009)ISTRAN +1009 FORMAT(' Searching strand',I6) + IDSAV=MAXMAT + CALL CFGEL(GELN,IDIMG,POSNS,IDIM,WORDP,WORDN,LENGTH,LPOWRC, + +SAVPG,SAVPS,SAVL, + +IDSAV,SEQ,GELCOP,MINMAT,IFAIL,KBOUT) + IF(IDSAV.GT.0) THEN + IMATCH=1 + CALL DISMAT(SEQ,IDIM,GELCOP,IDIMG,SAVPS,SAVPG,IDSAV, + + CENDS,NENDS,IDCEND,MAXCON,IDEV,MATCH) + END IF + IF(ISTRAN.EQ.1) THEN + CALL SQREV(GELCOP,IDIMG) + CALL SQCOM(GELCOP,IDIMG) + CALL SQCOPY(GELCOP,GEL,IDIMG) + ISTRAN = 2 + GO TO 2 + END IF + IF(IMATCH.EQ.0) THEN + WRITE(IDEV2,1010)NAME + IGELS = IGELS + 1 + END IF + GO TO 1 +1010 FORMAT(A) +100 CONTINUE + WRITE(KBOUT,*)'Batch finished' + WRITE(KBOUT,*)JGEL,' compared and ',IGELS,' passed' + RETURN + END + SUBROUTINE SHIFTC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDEVR, + +IDBSIZ,IGN,NCONT,DIST) +C AUTHOR: RODGER STADEN +C SHIFTS PART OF A CONTIG FORM GEL IGN TO RIGHT END +C CONTIG LINE NUMBER IF NCONT + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER DIST + I = IGN +10 CONTINUE + IF(I.NE.0)THEN + RELPG(I) = RELPG(I) + DIST + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + I = RNBR(I) + GO TO 10 + END IF +C UPDATE CONTIG LENGTH + RELPG(NCONT) = RELPG(NCONT) + DIST + CALL WRITER(IDEVR,NCONT,RELPG(NCONT),LNGTHG(NCONT), + +LNBR(NCONT),RNBR(NCONT)) + END + SUBROUTINE SLIDER(SEQ1,IDC,SEQ2,IDIM2,MS1,MS2,MAXPG,MAXPC,MINSLI, + +MATL,MATC,MATG,IP) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDC),SEQ2(IDIM2) + INTEGER MATL(IP),MATC(IP),MATG(IP),P1S,P1,P2 + IP1 = IP + IP = 0 +C LEFT END S2 RELATIVE S1 - MAX PADS -2 READY FOR LOOP + P1S = MS1 - MS2 - MAXPC - 1 +C TRY NSLIDE START POSNS FOR SEQ2 + DO 100 I=1,MAXPG+MAXPC+1 +C POINT TO SEQ1 START + P1S = P1S + 1 +C POINT TO CURRENT SEQ1 POSN + P1 = P1S + N = 0 +C COMPARE WHOLE LENGTH OF SEQ2 (IF P1 WITHIN RANGE) + DO 50 J=1,IDIM2 + P2 = J + P1 = P1 + 1 + IF(P1.LT.1)GO TO 50 +C OFF RIGHT END? IF SO MAY HAVE BEEN A MATCH + IF(P1.GT.IDC)GO TO 40 + IF(SEQ1(P1).EQ.SEQ2(P2))GO TO 45 +40 CONTINUE + IF(N.GE.MINSLI)CALL SAVIT(N,P1,P2,IP,MATL,MATC,MATG,IP1) + N = 0 + GO TO 50 +45 CONTINUE + N = N + 1 +50 CONTINUE +C GOOD SCORE AT END? NEED TO INCREMENT POINTERS FOR SAVIT + P1 = P1 + 1 + P2 = P2 + 1 + IF(N.GE.MINSLI)CALL SAVIT(N,P1,P2,IP,MATL,MATC,MATG,IP1) +100 CONTINUE + END + SUBROUTINE SUBS(SEQ,IDIMS,FROM,TO) + CHARACTER SEQ(IDIMS),FROM,TO +C AUTHOR RODGER STADEN + DO 10 I = 1,IDIMS + IF(SEQ(I).EQ.FROM) SEQ(I) = TO +10 CONTINUE + END + SUBROUTINE SUMMAR(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,LREG,RREG,IGELC,PERCD,IDBSIZ,CHARS, + +ID1,CHRSIZ,MAXGL2,IDEVW,MAXGEL,LINOU1,LINOU2,MXGOOD) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),CHRSIZ + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER SEQ1(IDIM1) + CHARACTER GEL(MAXGEL) + INTEGER LREG,RREG,LSEQNO,POSN,Y,POSN1 + INTEGER GELC + CHARACTER LINOU1(MAXGEL),LINOU2(MAXGEL),GTCONC + INTEGER CHARS(CHRSIZ,ID1,MAXGL2) + EXTERNAL INDEXS,LWRAPS,GTCONC +C 28-7-91 added extra parameter: mxgood is the maximum reading +C length for which we have confidence, so only the parts of +C reads 1 to mxgood will be included in the calculation +C SET INITIAL VALUES +C hard to understand this very old code! rewrite it. +C we have a summing array of twice the length of the longest sequence +C posn is posn in contig of next to write, lseqno is current posn in contig +C we write when lseqno-posn ge the length of the longest seq + POSN=LREG + GELC=IGELC + LINLEN=MAXGEL + LSEQNO=RELPG(GELC) + IEND=0 + DO 40 I=1,MAXGL2 + DO 40 J=1,ID1 + DO 40 K=1,CHRSIZ + CHARS(K,J,I)=0 +40 CONTINUE +50 CONTINUE + ISS=1 + IF(LNGTHG(GELC).LT.0)ISS=2 + CALL READW(IDEVW,GELC,GEL,MAXGEL) +C LOOP FOR RELEVANT ELEMENTS THIS GEL +C only use mxgood characters from start of read +C + IF(ISS.EQ.1) THEN + N = MIN(MXGOOD,ABS(LNGTHG(GELC))) + IF(LSEQNO.LT.LREG)LSEQNO=LREG + IS = LSEQNO-RELPG(GELC)+1 + ELSE +C LOOP FOR RELEVANT ELEMENTS THIS GEL +C only use mxgood characters from start of read (right end for these) +C + IREND = RELPG(GELC) - LNGTHG(GELC) + 1 + IF (MXGOOD.LT.MAXGEL) THEN + LSEQNO = IREND - MXGOOD + 1 + ELSE + LSEQNO = RELPG(GELC) + END IF + LSEQNO = MAX(LSEQNO,LREG) + IS = LSEQNO - RELPG(GELC) + 1 + N = ABS(LNGTHG(GELC)) + END IF + DO 70 I=IS,N + JJ = INDEXS(GEL(I),JSCORE) + JJJ = LWRAPS(LSEQNO,MAXGL2) + CHARS(JJ,ISS,JJJ) = CHARS(JJ,ISS,JJJ) + JSCORE + LSEQNO = LSEQNO + 1 +70 CONTINUE + IF(RNBR(GELC).EQ.0)GO TO 200 + GELC=RNBR(GELC) + LSEQNO=RELPG(GELC) + IF(LSEQNO.GT.RREG)GO TO 200 +C ENOUGH TO OUTPUT? + Y=LSEQNO-POSN + IF(Y.GE.MAXGEL)GO TO 210 + GO TO 50 +200 CONTINUE +C SET FLAG TO SHOW END REACHED + IEND=1 + LINLEN=MAXGEL + Y=RREG-POSN + IF(Y.LT.MAXGEL)LINLEN=Y+1 +210 CONTINUE +C SET POINTER TO SEQ1 + POSN1=POSN-1 +C PREPARE NEXT SECTION OF CHARS FOR OUTPUT + DO 230 I=1,LINLEN + JJJ = LWRAPS(POSN,MAXGL2) + LINOU1(I) = GTCONC(CHARS(1,1,JJJ),CHRSIZ,PERCD) + LINOU2(I) = GTCONC(CHARS(1,2,JJJ),CHRSIZ,PERCD) + DO 250 J=1,CHRSIZ + CHARS(J,1,JJJ)=0 + CHARS(J,2,JJJ)=0 +250 CONTINUE + POSN=POSN+1 +230 CONTINUE +C +C COMPARE STRANDS +C + DO 500 I=1,LINLEN +C WRITE(*,*)I,LINOU1(I),LINOU2(I) + POSN1=POSN1+1 + IF(LINOU1(I).EQ.LINOU2(I)) THEN + IF(LINOU1(I).EQ.'-') THEN + SEQ1(POSN1) = '3' + GO TO 500 + END IF + IF(LINOU1(I).EQ.'*') THEN + SEQ1(POSN1) = '3' + GO TO 500 + END IF + SEQ1(POSN1) = '0' + ELSE + IF((LINOU1(I).EQ.'*').AND.(LINOU2(I).EQ.'-')) THEN + SEQ1(POSN1) = '3' + GO TO 500 + END IF + IF((LINOU2(I).EQ.'*').AND.(LINOU1(I).EQ.'-')) THEN + SEQ1(POSN1) = '3' + GO TO 500 + END IF + IF((LINOU1(I).NE.'-').AND.(LINOU1(I).NE.'*')) THEN + SEQ1(POSN1) = '1' + IF((LINOU2(I).NE.'-').AND.(LINOU2(I).NE.'*')) + + SEQ1(POSN1) = '4' + GO TO 500 + END IF + IF((LINOU2(I).NE.'-').AND.(LINOU2(I).NE.'*')) THEN + SEQ1(POSN1) = '2' + IF((LINOU1(I).NE.'-').AND.(LINOU1(I).NE.'*')) + + SEQ1(POSN1) = '4' + GO TO 500 + END IF + END IF +500 CONTINUE + IF(POSN.GT.RREG)RETURN + IF((IEND.EQ.1).AND.(POSN.LE.RREG))GO TO 200 +C ANY MORE MAXGEL CHAR LENGTHS TO OUTPUT + Y=LSEQNO-POSN + IF(Y.LT.MAXGEL)GO TO 50 +C FINISHED COMPLETELY? + GO TO 210 + END +C SUMMER +C +C SUBROUTINE TO PRODUCE A CONSENSUS FROM LINED UP GEL READINGS + SUBROUTINE SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + 1SEQ1,IDIM1,GEL,LREG,RREG,IGELC,IDBSIZ,CHARS,CHRSIZ,MAXGL2, + +IDEVW,MAXGEL,IDM,PERCD) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),CHRSIZ + INTEGER LREG,RREG,LSEQNO,POSN,Y + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + INTEGER GELC + CHARACTER SEQ1(IDIM1) + INTEGER CHARS(CHRSIZ,MAXGL2) + CHARACTER MUNOTP,GTCONC + EXTERNAL MUNOTP,INDEXS,GTCONC,LWRAPS +C +C SET INITIAL VALUES + POSN=LREG + GELC=IGELC + LINLEN=MAXGEL + LSEQNO=RELPG(GELC) + IEND=0 + IPSEQ1=0 +C +C ZERO ARRAY + DO 40 I=1,MAXGL2 + DO 40 J=1,CHRSIZ + CHARS(J,I)=0 +40 CONTINUE +50 CONTINUE + CALL READW(IDEVW,GELC,GEL,MAXGEL) +C LOOP FOR RELEVANT ELEMENTS THIS GEL + N=ABS(LNGTHG(GELC)) + IF(LSEQNO.LT.LREG)LSEQNO=LREG + IS=(LSEQNO-RELPG(GELC))+1 +**************************** + IF(IDM.EQ.26)THEN + DO 51 I = IS,N + JJJ=(MOD(LSEQNO,MAXGL2)) + IF(JJJ.EQ.0)JJJ=MAXGL2 + CALL PCON1(GEL(I),CHARS(1,JJJ)) + LSEQNO = LSEQNO + 1 +51 CONTINUE + ELSE +**************************** + DO 70 I=IS,N + JJ = INDEXS(GEL(I),JSCORE) + JJJ = LWRAPS(LSEQNO,MAXGL2) + CHARS(JJ,JJJ) = CHARS(JJ,JJJ) + JSCORE + LSEQNO = LSEQNO + 1 +70 CONTINUE + END IF +C +C LOOK AT NEXT GEL TO RIGHT + IF(RNBR(GELC).EQ.0)GO TO 200 + GELC=RNBR(GELC) +C RESET LSEQNO + LSEQNO=RELPG(GELC) +C IS THIS OVER END? + IF(LSEQNO.GT.RREG)GO TO 200 +C ENOUGH TO OUTPUT? + Y=LSEQNO-POSN + IF(Y.GE.MAXGEL)GO TO 210 + GO TO 50 +200 CONTINUE +C SET FLAG TO SHOW END REACHED + IEND=1 +C NEED TO SUM AND OUTPUT + LINLEN=MAXGEL + Y=RREG-POSN + IF(Y.LT.MAXGEL)LINLEN=Y+1 +210 CONTINUE +C SUM NEXT SECTION OF CHARS + IF(IDM.EQ.26)THEN + DO 211 I = 1,LINLEN + IPSEQ1 = IPSEQ1 + 1 + SEQ1(IPSEQ1) = '-' + JJJ = MOD(POSN,MAXGL2) + IF(JJJ.EQ.0)JJJ = MAXGL2 + SEQ1(IPSEQ1) = MUNOTP(CHARS(1,JJJ)) + CHARS(1,JJJ) = 0 + POSN = POSN + 1 +211 CONTINUE + ELSE + DO 230 I=1,LINLEN + IPSEQ1=IPSEQ1+1 + ISUM=0 + JJJ = LWRAPS(POSN,MAXGL2) + SEQ1(IPSEQ1) = GTCONC(CHARS(1,JJJ),CHRSIZ,PERCD) + CALL FILLI(CHARS(1,JJJ),CHRSIZ,0) + POSN = POSN + 1 +230 CONTINUE + END IF +C +C +C ANY MORE TO OUTPUT? + IF(POSN.GT.RREG)RETURN + IF((IEND.EQ.1).AND.(POSN.LE.RREG))GO TO 200 +C ANY MORE MAXGLEL CHAR LENGTHS TO OUTPUT + Y=LSEQNO-POSN + IF(Y.LT.MAXGEL)GO TO 50 +C FINISHED COMPLETELY? + GO TO 210 + END + SUBROUTINE TPCHEK(PC,PG,L,N) + INTEGER PC(N),PG(N),L(N) +C AUTHOR RODGER STADEN +C IF OVERLAPPING BLOCKS ARE FOUND REMOVE THE SHORTER ONE +C THEN REMOVE LARGE GAPS AT ENDS (THOSE AS LARGE AS THE END BLOCK) + K1 = 2 +1 CONTINUE + DO 10 I = K1,N + J1 = I + IF(PC(I).LE.PC(I-1)) GO TO 20 + IF(PG(I).LE.PG(I-1)) GO TO 20 +10 CONTINUE +C REMOVE LARGE GAPS FROM ENDS +C THIS RULE OF THUMB COULD BE CHANGED TO USE A DIFFERENCE +C BETWEEN THE NUMBERS OF MISMATCHING CHARACTERS + IF(N.GT.1) THEN + K1 = PC(2) - PC(1) - L(1) + J1 = PG(2) - PG(1) - L(1) + IF(MAX(K1,J1).GT.L(1)) THEN + CALL ML(PC,PG,L,N,1) + N = N - 1 + END IF + IF(N.GT.1) THEN + K1 = PC(N) - PC(N-1) - L(N-1) + J1 = PG(N) - PG(N-1) - L(N-1) + IF(MAX(K1,J1).GT.L(N)) THEN + CALL ML(PC,PG,L,N,N) + N = N - 1 + END IF + END IF + END IF + RETURN +20 CONTINUE + IF(L(J1-1).GT.L(J1)) THEN + CALL ML(PC,PG,L,N,J1) + ELSE + CALL ML(PC,PG,L,N,J1-1) + END IF +C Until 25-11-90 next line was k1=j1 but this does not deal with all +C cases: when a line is deleted we must compare it with the previous +C one before dealing with the rest, because it could be left of that +C one as well! + K1 = MAX(2,J1-1) + N = N - 1 + GO TO 1 + END + SUBROUTINE WRITEN(IDEVN,N,NAME) + CHARACTER NAME*(*) + WRITE(IDEVN,REC=N)NAME + RETURN + END + SUBROUTINE WRITER(IDEVR,N,RELPG,LNGTHG,LNBR,RNBR) + INTEGER RELPG,RNBR + WRITE(IDEVR,REC=N)RELPG,LNGTHG,LNBR,RNBR + RETURN + END + SUBROUTINE WRITEW(IDEVW,N,GEL,MAXGEL) + CHARACTER GEL(MAXGEL) + WRITE(IDEVW,REC=N)GEL + RETURN + END + SUBROUTINE XHSAP(RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,NCONTS,LLINOI,LINCNI,LREG,RREG, + +WINDOW,GWIND,LENCON,DEPTHP,DEPTHM, + +MARGL,MARGR,MARGB,MARGT,MAXOPT,ISXMAX,ISYMAX,KBIN,IDEV, + +KBOUT,GEL,GEL2,IDEV2,IDEV3,LINLEN,PERCD,MAXGEL,IDM, + +SEQ1,IDIM1,NGELS,TEMP3,CHRSIZ,MAXGL2,LINOU1,LINOU2, + +NOPT1,NOPT2,NOPT3, + +IHELPS,IHELPE,HELPF,IDEVH,MXGOOD) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),WINDOW,CHRSIZ,GWIND + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER MARGB(MAXOPT),MARGT(MAXOPT) + INTEGER RREG,DEPTHP,DEPTHM,STRAND,CHNRP1,HQN + INTEGER TEMP3(2,CHRSIZ,MAXGL2) + CHARACTER GEL(MAXGEL),GEL2(MAXGEL) + CHARACTER TERM,TUPPER,NAMARC*10,HELPF*(*) + CHARACTER SEQ1(IDIM1),LINOU1(MAXGEL),LINOU2(MAXGEL) + EXTERNAL NOPWIN,CWORLD,TUPPER,CHNRP1,HQN +C nopt1 = single contig +C nopt2 = all contigs +C nopt3 = scan +10 CONTINUE + LLINO = LLINOI + LINCON = LINCNI + LOCLR = 0 + LOCRR = 0 + CALL BPAUSE(KBIN,KBOUT,IOK) + CALL CLEARV + CALL XHAIRR(ISXMAX,ISYMAX,IX,IY,TERM,DBTDUX,DBTDUY) + CALL VT100M + INFLAG = HQN(TERM) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.3) RETURN + NOPT = NOPWIN(IY,MARGB,MARGT,MAXOPT) + TERM = TUPPER(TERM) + IF(NOPT.EQ.0) RETURN + IF(NOPT.EQ.NOPT3) THEN + IF(TERM.EQ.'S') THEN + XMIN = LREG + XMAX = RREG + X = CWORLD(IX,MARGL,MARGR,XMIN,XMAX) + LOCLR = MAX(LREG,NINT(X)-WINDOW) + LOCRR = MIN(RREG,NINT(X)+WINDOW-1) + IF(LOCLR.NE.0) THEN + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + + GEL,LLINO,LINCON,LOCLR,LOCRR,GEL2,I1,I2,0,I, + + IDBSIZ,IDEV,KBOUT, + + IDEV2,IDEV3,LINLEN,PERCD,MAXGEL,IDM) + GO TO 10 + END IF + END IF + IF((TERM.EQ.'N').OR.(TERM.EQ.'Z').OR.(TERM.EQ.'I')) GO TO 10 + END IF + IF(NOPT.EQ.NOPT1) THEN + STRAND = 1 + CALL FDPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON,STRAND,DEPTHP) + IF(DEPTHP.LT.0) RETURN + STRAND = -1 + CALL FDPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON,STRAND,DEPTHM) + IF(DEPTHM.LT.0) RETURN + YMAX = ISYMAX + YMIN = 0. + XMIN = LREG + XMAX = RREG + RINC = ISYMAX / (DEPTHP + DEPTHM + 2) + RMID =(DEPTHM+1) * RINC + X = CWORLD(IX,MARGL,MARGR,XMIN,XMAX) + Y = CWORLD(IY,MARGB(NOPT),MARGT(NOPT),YMIN,YMAX) + IF(TERM.EQ.'I') THEN + STRAND = 1 + YINC = RINC * STRAND + CALL IPLTC(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,STRAND, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + + ISXMAX,ISYMAX,RMID,YINC,DEPTHP,X,Y, + + KBOUT,IGEL,ICLOSE) + IF(ICLOSE.EQ.1) THEN + STRAND = -1 + YINC = RINC * STRAND + CALL IPLTC(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,STRAND, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + + ISXMAX,ISYMAX,RMID,YINC,DEPTHM,X,Y, + + KBOUT,IGEL,ICLOSE) + END IF + IF(ICLOSE.EQ.1) GO TO 10 + CALL READN(IDEV3,IGEL,NAMARC) + WRITE(IDEV,1006)NAMARC,IGEL,RELPG(IGEL),LNGTHG(IGEL) +1006 FORMAT + + ( ' Name ',A,' Number ',I4,' Rel. Posn. ',I7,' Length ',I5) + GO TO 10 + END IF + IF(TERM.EQ.'Z') THEN + STRAND = 1 + YINC = RINC * STRAND + CALL IPLTC(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,STRAND, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + + ISXMAX,ISYMAX,RMID,YINC,DEPTHP,X,Y, + + KBOUT,IGEL,ICLOSE) + IF(ICLOSE.EQ.1) THEN + STRAND = -1 + YINC = RINC * STRAND + CALL IPLTC(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,STRAND, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + + ISXMAX,ISYMAX,RMID,YINC,DEPTHM,X,Y, + + KBOUT,IGEL,ICLOSE) + END IF + IF(IGEL.EQ.0) GO TO 10 + CALL CLEARG + CALL PLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LINCON,IGEL, + + NCONTS,MARGL,MARGR,MARGB(NOPT2),MARGT(NOPT2),ISXMAX,ISYMAX) + LREG = MAX(1,RELPG(IGEL)-GWIND) + RREG = MIN(RELPG(LINCON),RELPG(IGEL)+GWIND) + LLINO = LNBR(LINCON) + LLINOI = LLINO + LINCNI = LINCON + LENCON = RREG - LREG + 1 + CALL FDEPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON, + + MARGL,MARGR,MARGB(NOPT1),MARGT(NOPT1),ISXMAX,ISYMAX) + GO TO 10 + END IF + IF(TERM.EQ.'S') THEN + LOCLR = MAX(LREG,NINT(X)-WINDOW) + LOCRR = MIN(RREG,NINT(X)+WINDOW-1) + IF(LOCLR.NE.0) THEN + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + + GEL,LLINO,LINCON,LOCLR,LOCRR,GEL2,I1,I2,0,I, + + IDBSIZ,IDEV,KBOUT, + + IDEV2,IDEV3,LINLEN,PERCD,MAXGEL,IDM) + GO TO 10 + END IF + END IF + IF(TERM.EQ.'N') THEN + LOCLR = MAX(LREG,NINT(X)-WINDOW) + LOCRR = MIN(RREG,NINT(X)+WINDOW-1) + IGEL = CHNRP1(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ, + + LLINO,LREG) + IF(LOCLR.NE.0) THEN + CALL LSTCON(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,IGEL, + + LOCRR,IDEV,IDEV3,NAMARC) + END IF + GO TO 10 + END IF + IF(TERM.EQ.'Q') THEN + CALL DBSCNP(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1, + + IDIM1,GEL,IDBSIZ,TEMP3,2,CHRSIZ,MAXGL2,IDEV2,LLINO, + + PERCD,MAXGEL,LINOU1,LINOU2,LREG,RREG, + + MARGL,MARGR,MARGB(NOPT3),MARGT(NOPT3),ISXMAX,ISYMAX, + + MXGOOD) + GO TO 10 + END IF + END IF + IF(NOPT.EQ.NOPT2) THEN + CALL IDPLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ, + + NCONTS,IX,IY,MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + + ISXMAX,ISYMAX,DBTDUX,DBTDUY, + + LINCON,IGEL,IS) + IF(IGEL.EQ.0) RETURN + IF(TERM.EQ.'Z') THEN + CALL CLEARG + CALL PLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LINCON,IGEL, + + NCONTS,MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX) + LREG = 1 + RREG = RELPG(LINCON) + LLINO = LNBR(LINCON) + LLINOI = LLINO + LINCNI = LINCON + LENCON = RREG - LREG + 1 + CALL FDEPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON, + + MARGL,MARGR,MARGB(NOPT1),MARGT(NOPT1),ISXMAX,ISYMAX) + GO TO 10 + END IF + IF(TERM.EQ.'Q') THEN + CALL CLEARG + CALL PLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LINCON,IGEL, + + NCONTS,MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX) + LREG = 1 + RREG = RELPG(LINCON) + LLINO = LNBR(LINCON) + LLINOI = LLINO + LINCNI = LINCON + LENCON = RREG - LREG + 1 + CALL FDEPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON, + + MARGL,MARGR,MARGB(NOPT1),MARGT(NOPT1),ISXMAX,ISYMAX) + CALL DBSCNP(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1, + + IDIM1,GEL,IDBSIZ,TEMP3,2,CHRSIZ,MAXGL2,IDEV2,LLINO, + + PERCD,MAXGEL,LINOU1,LINOU2,LREG,RREG, + + MARGL,MARGR,MARGB(NOPT3),MARGT(NOPT3),ISXMAX,ISYMAX, + + MXGOOD) + GO TO 10 + END IF + IF(TERM.EQ.'I') THEN + CALL READN(IDEV3,IGEL,NAMARC) + WRITE(IDEV,1006)NAMARC,IGEL,RELPG(IGEL),LNGTHG(IGEL) + GO TO 10 + END IF + IF(TERM.EQ.'S') THEN + LOCLR = MAX(1,IS-WINDOW) + LOCRR = MIN(RELPG(LINCON),IS+WINDOW-1) + LLINO = LNBR(LINCON) + IF(LOCLR.NE.0) THEN + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + + GEL,LLINO,LINCON,LOCLR,LOCRR,GEL2,I1,I2,0,I, + + IDBSIZ,IDEV,KBOUT, + + IDEV2,IDEV3,LINLEN,PERCD,MAXGEL,IDM) + END IF + GO TO 10 + END IF + IF(TERM.EQ.'N') THEN + LOCLR = MAX(1,IS-WINDOW) + LOCRR = MIN(RELPG(LINCON),IS+WINDOW-1) + LLINO = LNBR(LINCON) + IF(LOCLR.NE.0) THEN + CALL LSTCON(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,IGEL, + + LOCRR,IDEV,IDEV3,NAMARC) + END IF + GO TO 10 + END IF + END IF + END + INTEGER FUNCTION CLINNO(LNBR,IDBSIZ,NCONTS,IIN) +C AUTHOR: RODGER STADEN +C RETURNS CONTIG LINE NUMBER OR ZERO FOR ERROR + INTEGER LNBR(IDBSIZ) + CLINNO = 0 + N=IDBSIZ-NCONTS + DO 10 J=N,IDBSIZ-1 + IF(LNBR(J).EQ.IIN) THEN + CLINNO = J + RETURN + END IF +10 CONTINUE + END diff --git a/src/staden/dbsysnew.f b/src/staden/dbsysnew.f new file mode 100644 index 0000000..0a08d13 --- /dev/null +++ b/src/staden/dbsysnew.f @@ -0,0 +1,2963 @@ +C 8-4-92 removed minimum block length changing option in dbauto +C 29.05.91 IMPLEMENTED REMOVE GEL READING +C 21-8-91 Added routines to find internal overlaps +C 2-9-91 Fixed bug in copytg +C 8-11-91 fixed bugs in "find internal joins" +C 24-02-92 fixed some bugs courtesy of Kozlowski's AIX port +C 23-Jun-92 COPYCC - params to READCC and WRITCC in wrong order +C +C SUBROUTINE TO ENTER NEW GEL SEQUENCES INTO DATA BASE. +C IT READS IN AN ARCHIVE VERSION AND WRITES OUT A WORKING VERSION. +C IT ALSO SETS UP ANY RELATIONSHIPS WITH OTHER DATA IN THE DATABASE +C BOTH BY POSITION IN A CONTIG AND POINTERS TO LEFT AND RIGHT +C NEIGHBOURS. + SUBROUTINE AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,NAMARC,X,ITYPE,ISENSE,SEQC2,ITOTPC, + +IDIM,IDC,NCONTC,LINCON,IFAIL,IDBSIZ,KBOUT,IDEVR,IDEVW,IDEVN, + +IDEVT,IDEVC,IDEVG,MAXGEL) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),X,Y + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),NAMARC*(*) + CHARACTER SEQC2(IDC) + CHARACTER NAMARK*10 +C WRITE(*,*)'X,ITYPE,ISENSE,IDIM,IDC' +C WRITE(*,*)X,ITYPE,ISENSE,IDIM,IDC +C SET FAIL FLAG + IFAIL=0 +C WRITE(KBOUT,1000) +C1000 FORMAT(' TRYING TO ENTER NEW GEL READING INTO DATABASE') +C IS THERE SPACE? + IF((IDBSIZ-(NGELS+NCONTS)).GT.2)GO TO 5 +C FULL + WRITE(KBOUT,1999)IDBSIZ +1999 FORMAT(' Database full, current size=',I6,' Extend with copy') + IFAIL=7 + RETURN +5 CONTINUE +C NEED TO CHECK TO SEE IF GEL ALREADY IN DB +C LOOK THRU ARC FILE + DO 10 J=1,NGELS +C READ(IDEVN,REC=J)NAMARK + CALL READN(IDEVN,J,NAMARK) + IF(NAMARK.NE.NAMARC(1:10))GO TO 10 +C FOUND + WRITE(KBOUT,1013)J +1013 FORMAT(' New gel already in database with number',I6, + +' Entry aborted') + IFAIL=6 + RETURN +10 CONTINUE +C INCREMENT NUMBER OF GELS + NGELS=NGELS+1 +C SET LENGTH THIS GEL + LNGTHG(NGELS)=IDIM*ISENSE +C WRITE NAME OF ARCHIVE TO LIST OF ARCHIVES +C NAMPRO,ARC +C WRITE(IDEVN,REC=NGELS)NAMARC(1:10) + NAMARK=NAMARC(1:10) + CALL WRITEN(IDEVN,NGELS,NAMARK) + WRITE(KBOUT,1003)NGELS +1003 FORMAT(' This gel reading has been given the number ',I6) +C WRITE GEL TO WORKING VERSION + CALL WRITEW(IDEVW,NGELS,GEL,MAXGEL) + IF(IDEVT.GT.0) CALL ENTRD(IDEVG,IDEVT,IDEVC,NAMARC,NGELS,IOK) +C CREATE TAGS FOR THIS NASTY + CALL TAGGEL(NGELS,LNGTHG(NGELS),GEL) +C SET UP RELATIONSHIPS +C DOES THIS GEL OVERLAP? + IF(ITYPE.NE.0)GO TO 100 +C +C DOES NOT OVERLAP SO IT STARTS A CONTIG OF ITS OWN +C SET LEFT AND RIGHT POINTERS TO ZERO,RELPG TO 1 + LNBR(NGELS)=0 + RNBR(NGELS)=0 + RELPG(NGELS)=1 +C WRITE NEW GEL LINE + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) +C WRITE(IDEVR,REC=NGELS)RELPG(NGELS),LNGTHG(NGELS),LNBR(NGELS), +C 1RNBR(NGELS) +C +C SET CONTIG POINTERS AND GENERAL VALUES +C INCREMENT NUMBER OF CONTIGS + NCONTS=NCONTS+1 +C POINTER TO THIS CONTIG + N=IDBSIZ-NCONTS +C POINTER TO LEFT GEL THIS CONTIG + LNBR(N)=NGELS +C POINTER TO RIGHT GEL THIS CONTIG + RNBR(N)=NGELS +C LENGTH OF CONTIG + RELPG(N)=IDIM +C WRITE CONTIG DESCRIPTOR + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) +C WRITE(IDEVR,REC=N)RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) +C WRITE DB DESCRIPTOR + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) +C WRITE(IDEVR,REC=IDBSIZ)NGELS,NCONTS + RETURN +C +100 CONTINUE +C +C +C DOES OVERLAP +150 CONTINUE +C +C LEFT END OR RIGHT OVERLAP? + IF(ITYPE.EQ.1)GO TO 400 +C RIGHT END OR INTERNAL OVERLAP +C +160 CONTINUE +C NEED TO SEARCH THRU THIS CONTIG TO FIND LEFT AND RIGHT +C NEIGHBOURS FOR THIS NEW GEL +C LINE NUMBER OF LEFT END OF CONTIG + N=NCONTC +C LOOK THRU UNTIL CURRENT IS >= THEN IT MUST BE THE PREVIOUS ONE +200 CONTINUE + IF(RELPG(N).GT.X)GO TO 250 +C IS THIS THE LAST GEL IN CONTIG? + IF(RNBR(N).EQ.0)GO TO 350 +C NO SO LOOK AT NEXT + N=RNBR(N) + GO TO 200 +250 CONTINUE +C GEL LIES BETWEEN N AND LNBR(N) +C NEED TO EDIT DB HERE + IF(ITOTPC.GT.0)CALL ABEDIN(RELPG,LNGTHG,LNBR,RNBR, + 1NGELS,NCONTS, + 2GEL,LINCON,X,SEQC2,ITOTPC,IDC,IDBSIZ,KBOUT,IDEVR,IDEVW, + +MAXGEL) +C +C +C SET POINTERS IN NEW GEL + LNBR(NGELS)=LNBR(N) + RNBR(NGELS)=N + RELPG(NGELS)=X +C WRITE NEW GEL LINE + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) +C WRITE(IDEVR,REC=NGELS)RELPG(NGELS),LNGTHG(NGELS),LNBR(NGELS), +C 1RNBR(NGELS) +C SET POINTERS IN LEFT AND RIGHT NEIGHBOURS + K=LNBR(N) + RNBR(K)=NGELS +C RNBR(LNBR(N))=NGELS +C WRITE LEFT AND RIGHT NEIGHBOURS + CALL WRITER(IDEVR,K,RELPG(K),LNGTHG(K), + +LNBR(K),RNBR(K)) +C WRITE(IDEVR,REC=K)RELPG(K),LNGTHG(K),LNBR(K),RNBR(K) + LNBR(N)=NGELS + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) +C WRITE(IDEVR,REC=N)RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) +C WRITE NGELS NCONTS + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) +C WRITE(IDEVR,REC=IDBSIZ)NGELS,NCONTS +C HAVE WE INCREASED LENGTH OF CONTIG? +C ITS LINE NUMBER IS LINCON +C NEED TO UPDATE IDIM IN CASE OF EDITS + IDIM=ABS(LNGTHG(NGELS)) + Y=X+IDIM-1 + IF(Y.LE.RELPG(LINCON))RETURN + RELPG(LINCON)=Y +C WRITE NEW CONTIG LINE + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) +C WRITE(IDEVR,REC=LINCON)RELPG(LINCON),LNGTHG(LINCON),LNBR(LINCON), +C 1RNBR(LINCON) + RETURN +350 CONTINUE +C MUST BE A RIGHT END OVERLAP +C NEED TO EDIT DB HERE + IF(ITOTPC.GT.0)CALL ABEDIN(RELPG,LNGTHG,LNBR,RNBR, + 1NGELS,NCONTS, + 2GEL,LINCON,X,SEQC2,ITOTPC,IDC,IDBSIZ,KBOUT,IDEVR,IDEVW, + +MAXGEL) +C +C +C SET POINTERS FOR NEW GEL + LNBR(NGELS)=N + RNBR(NGELS)=0 + RELPG(NGELS)=X +C WRITE NEW GEL LINE + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) +C WRITE(IDEVR,REC=NGELS) +C 1RELPG(NGELS),LNGTHG(NGELS),LNBR(NGELS),RNBR(NGELS) +C OLD RIGHT END + RNBR(N)=NGELS +C WRITE NEW RIGHT LINE + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) +C WRITE(IDEVR,REC=N)RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) +C +C RESET RIGHT NAME IN CONTIG +C ITS LINE NUMBER IS LINCON + RNBR(LINCON)=NGELS +C HAVE WE INCREASED LENGTH OF CONTIG? +C NEED TO UPDATE LENGTH OF GEL IN CASE OF EDITS + IDIM=ABS(LNGTHG(NGELS)) + Y=X+IDIM-1 + RELPG(LINCON)=MAX(RELPG(LINCON),Y) +C WRITE HERE +C WRITE CONTIG DESCRIPTOR + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) +C WRITE(IDEVR,REC=LINCON)RELPG(LINCON),LNGTHG(LINCON),LNBR(LINCON) +C 1,RNBR(LINCON) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) +C WRITE(IDEVR,REC=IDBSIZ)NGELS,NCONTS + RETURN +C +400 CONTINUE +C +C ADDING TO LEFT END +410 CONTINUE +C NEED TO EDIT DB HERE + IF(ITOTPC.GT.0)CALL ABEDIN(RELPG,LNGTHG,LNBR,RNBR, + 1NGELS,NCONTS, + 2GEL,LINCON,1,SEQC2,ITOTPC,IDC,IDBSIZ,KBOUT,IDEVR,IDEVW, + +MAXGEL) +C +420 CONTINUE +C SET POINTERS IN NEW GEL + RELPG(NGELS)=1 + RNBR(NGELS)=NCONTC + LNBR(NGELS)=0 +C WRITE NEW GEL LINE + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) +C WRITE(IDEVR,REC=NGELS)RELPG(NGELS),LNGTHG(NGELS),LNBR(NGELS), +C 1RNBR(NGELS) +C SET POINTERS IN OLD LEFT END + LNBR(NCONTC)=NGELS + RELPG(NCONTC)=X +C WRITE NEW LEFT END + CALL WRITER(IDEVR,NCONTC,RELPG(NCONTC),LNGTHG(NCONTC), + +LNBR(NCONTC),RNBR(NCONTC)) +C WRITE(IDEVR,REC=NCONTC)RELPG(NCONTC),LNGTHG(NCONTC),LNBR(NCONTC) +C 1,RNBR(NCONTC) +C NEW LENGTH OF CONTIG + RELPG(LINCON)=RELPG(LINCON)+X-1 +C MAY HAVE JUST ADDED A GEL LONGER THAN CONTIG + IDIM=ABS(LNGTHG(NGELS)) + Y=IDIM + IF(Y.GT.RELPG(LINCON))RELPG(LINCON)=Y +C NEW NAME OF LEFT END OF CONTIG + LNBR(LINCON)=NGELS +C WRITE CONTIG DESCRIPTOR + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) +C WRITE(IDEVR,REC=LINCON)RELPG(LINCON),LNGTHG(LINCON),LNBR(LINCON) +C 1,RNBR(LINCON) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) +C WRITE(IDEVR,REC=IDBSIZ)NGELS,NCONTS +C NOW GO THRU AND CHANGE ALL RELATIVE POSITIONS + N=NCONTC +440 CONTINUE + IF(RNBR(N).EQ.0)RETURN + N=RNBR(N) + RELPG(N)=RELPG(N)+X-1 +C WRITE NEW LINE + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) +C WRITE(IDEVR,REC=N)RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) + GO TO 440 + END +C SUBROUTINE COPYRD(IDEVRD,IDEV,NGELS,NEWSIZ,IOK) +C CHARACTER MTYPE*4,NAMFIL*12 +C DO 10 I = 1,NGELS +C CALL READRD(IDEVRD,I,LENR,LCUT,LENW,MTYPE,NAMFIL) +C CALL WRITRD(IDEV,I,LENR,LCUT,LENW,MTYPE,NAMFIL) +C 10 CONTINUE +C CALL WRITRD(IDEV,NEWSIZ,LENR,LCUT,LENW,MTYPE,NAMFIL) +C IOK = 0 +C END + SUBROUTINE COPYTG(IDEVT,IDEV,IOK,IDBSIZ,NEWSIZ,NGELS) +C Read tag details + IDIFF = NEWSIZ - IDBSIZ + CALL READTG(IDEVT,IDBSIZ,ICNT,LLEN,LCOM,LTYPE,NEXT) + IF (NEXT.NE.0) NEXT = NEXT + IDIFF + CALL WRITTG(IDEV,NEWSIZ,ICNT+IDIFF,LLEN,LCOM,LTYPE,NEXT) +C Copy headers for each gels + DO 10 I = 1,NGELS + CALL READTG(IDEVT,I,LPOS,LLEN,LCOM,LTYPE,NEXT) + IF (NEXT.NE.0) NEXT = NEXT + IDIFF + CALL WRITTG(IDEV,I,LPOS,LLEN,LCOM,LTYPE,NEXT) + 10 CONTINUE +C Copy rest of tags + DO 20 I = IDBSIZ+1, ICNT + CALL READTG(IDEVT,I,LPOS,LLEN,LCOM,LTYPE,NEXT) + IF (NEXT.NE.0) NEXT = NEXT + IDIFF + CALL WRITTG(IDEV,I+IDIFF,LPOS,LLEN,LCOM,LTYPE,NEXT) + 20 CONTINUE + IOK = 0 + END + SUBROUTINE COPYCC(IDEVC,IDEV,IOK) +C COMMENT_LENGTH + CHARACTER NOTE*40 + CALL READCC(IDEVC,1,ICNT,NEXT,NOTE) + CALL WRITCC(IDEV,1,ICNT,NEXT,NOTE) + DO 10 I = 2,ICNT + CALL READCC(IDEVC,I,ICNT,NEXT,NOTE) + CALL WRITCC(IDEV,I,ICNT,NEXT,NOTE) + 10 CONTINUE + IOK = 0 + END + SUBROUTINE DBAUTO(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ, + +NGELS,NCONTS,MAXGEL, + +TEMP3,WORDP,WORDN,LPOWRC,POSNS,GELN, + +SEQ1,MAXSEQ,SEQ2,SEQ3,SEQ4,SEQ5,SEQC2,SEQG2,MATCH, + +MAXGLM,MAXGL2,CHRSIZ,ECHRSZ,LENGTH, + +SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON,CONST, + +KBIN,KBOUT,IDEV1,IDEV2,IDEV3,IDEV4,IDEV7,IDEV8,IDEV,IDEVT,IDEVC, + +IHELPS,IHELPE,HELPF,IDEVH,NAMARC,NAMPRO,FILE, + +PERCD,IOPEN,IDM,SEQG3,SEQC3,IOK) + INTEGER CHRSIZ,ECHRSZ + INTEGER RELPG(MAXDB),PL(2),PR(2),RMOST + INTEGER LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + INTEGER JOINT(2),ITOTPC(2),ITOTPG(2),IDIM22(2),IDOUT(2) + INTEGER LINCON(2),LLINO(2),ITYPE(2),IFAIL(2) + INTEGER ILEFTS(2),ILC(2),IPOSC(2),IPOSG(2),ISENSE(2) + INTEGER LREG,RREG,X,ANS,ANSJOK + INTEGER TEMP3(ECHRSZ,MAXGL2),CONST(LENGTH) + INTEGER POSNS(MAXSEQ),WORDP(LPOWRC),WORDN(LPOWRC),GELN(MAXGLM) + INTEGER CENDS(MAXCON),NENDS(MAXCON) + CHARACTER SEQ3(MAXGLM),SEQC2(MAXGLM,2),SEQG2(MAXGLM,2) + CHARACTER SEQ1(MAXSEQ),SEQ2(MAXGLM),MATCH(MAXGLM),SEQ4(MAXGLM) + INTEGER SAV1(MAXSAV),SAV2(MAXSAV),SAV3(MAXSAV) + CHARACTER NAMARC*(*),NAMPRO*(*),FILE*(*) + CHARACTER GET,SEQ5(MAXGLM),HELPF*(*),SEQG3(MAXGLM),SEQC3(MAXGLM) + PARAMETER (MAXPRM = 32) + CHARACTER PROMPT(3)*(MAXPRM) + SAVE GET + DATA GET/'>'/ + WRITE(KBOUT,*)' Automatic sequence assembler' + IFAIL(1) = 0 + IEMPTY=0 + IF(NGELS.LT.1)IEMPTY=1 + CALL DBCHEK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +TEMP3,IERR,KBOUT) + IF(IERR.GT.1) RETURN + CALL YESNO(IOKENT,'Permit entry', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOKENT.LT.0) RETURN + CALL YESNO(INF,'Use file of file names', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(INF.LT.0) RETURN + IF(INF.EQ.0) THEN + FILE = ' ' + CALL OPENF1(IDEV7,FILE,0,IOK,KBIN,KBOUT, + + 'File of gel reading names', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + END IF + IF(IOKENT.EQ.0) THEN + FILE = ' ' + CALL OPENF1(IDEV8,FILE,1,IOK,KBIN,KBOUT, + + 'File for names of failures', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + END IF + PROMPT(1) = 'Perform normal shotgun assembly' + PROMPT(2) = 'Put all sequences in one contig' + PROMPT(3) = 'Put all sequences in new contigs' + IOPT = 1 + CALL RADION('Select entry mode',PROMPT,3,IOPT, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOPT.LT.1) RETURN + IF(IOPT.EQ.1) THEN +C parameters for normal assembly + ANSJOK = 0 + CALL YESNO(ANSJOK,'Permit joins', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANSJOK.LT.0) RETURN + MN = LENGTH*2 + MX = MAXGLM + 1 + MINMAT = MAX(15,MN) + CALL GETINT(MN,MX,MINMAT, + +'Minimum initial match', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINMAT = IVAL + MINSLI = 3 + MN = 0 + MX = 25 + MAXPG = 8 + CALL GETINT(MN,MX,MAXPG, + +'Maximum pads per gel', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXPG = IVAL + MN = 0 + MX = 25 + MAXPC = 8 + CALL GETINT(MN,MX,MAXPC, + +'Maximum pads per gel in contig', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXPC = IVAL + IF(IOKENT.EQ.0) THEN + RMN = 0. + RMX = 15. + PERMAX = 8. + CALL GETRL(RMN,RMX,PERMAX, + + 'Maximum percent mismatch after alignment', + + VAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + PERMAX = VAL + END IF + IDIM1=0 + MAXOVR=MAXGEL-3*MAX(MAXPC,MAXPG) + ANS=0 + IF(IEMPTY.EQ.0) + +CALL ACONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,MAXSEQ,SEQ2,IDBSIZ,IDIM1,ANS,KDUMM,KDUMM,KDUMM,TEMP3, + +ECHRSZ,MAXGL2,KBOUT,IDEV2,IFAIL(1),MAXGEL,IDM,PERCD) + END IF + JGEL = 0 + JNGEL = 0 + JNJOIN = 0 + IMATC = 0 + IF(IFAIL(1).NE.0)GO TO 900 +C +C +1 CONTINUE +C +C + IDIM2=MAXGEL + IF(INF.EQ.1) THEN +3 CONTINUE + MN = 0 + CALL GTSTR('Gel reading name',' ',NAMARC,MN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.3) RETURN + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 3 + END IF + ELSE + READ(IDEV7,1002,END=900)NAMARC + END IF +1002 FORMAT( A) + DO 77 MM=1,80 + MATCH(MM)=GET +77 CONTINUE + WRITE(IDEV,1077)(MATCH(KK),KK=1,79) +1077 FORMAT(' ',79A1) + JGEL = JGEL + 1 + WRITE(IDEV,*)'Processing',JGEL,' in batch' +1007 FORMAT(' Gel reading name=',A) + WRITE(IDEV,1007)NAMARC + CALL OPENRS(IDEV4,NAMARC,IOK,LRECL,2) + IF(IOK.NE.0)THEN + IF(INF.EQ.1) RETURN + WRITE(IDEV,*)' Error opening gel reading file' + GO TO 1 + END IF + CALL ARRFIM(IDEV4,SEQ2,IDIM2,KBOUT) + CLOSE(UNIT=IDEV4) + WRITE(IDEV,1800)IDIM2 +1800 FORMAT(' Gel reading length=',I6) + IF(IOPT.NE.1) THEN + CALL DBAUTP(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + SEQ2,NAMARC,JOINT,ITYPE,ISENSE,SEQC2,ITOTPC, + + IDIM2,IDOUT,LLINO,LINCON,IFAIL,IDBSIZ,MAXDB,IDEV, + + IDEV1,IDEV2,IDEV3,IDEVT,IDEVC,IDEV4,MAXGEL,IMATC,IEMPTY,IOPT) + IF(IFAIL(1).NE.0) GO TO 800 + JNGEL = JNGEL + 1 + GO TO 1 + END IF + IF(IDIM2.LT.MINMAT)THEN + WRITE(IDEV,*) + +' Reading shorter than minimum match, so not processed' + GO TO 1 + END IF + CALL SQCOPY(SEQ2,SEQ3,IDIM2) + IFCOMP=0 + IMATC=0 + IF(IEMPTY.EQ.0) + +CALL AUTOCN(SEQ1,IDIM1,SEQ2,IDIM2,ILEFTS,ILC,IPOSC, + +IPOSG,ISENSE,LLINO,IMATC,IFCOMP,MINMAT,POSNS,WORDP,WORDN, + +CONST,LENGTH,LPOWRC,IDEV,MATCH,MAXGEL,MAXGLM,SEQ5,GELN, + +SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON, + +SEQG2,SEQC2,SEQ4,IDOUT,IDIM22,ITOTPG,ITOTPC,JOINT,IFAIL, + +ITYPE,MAXPC,MAXPG,PERMAX,MINSLI,SEQG3,SEQC3,KFAIL) + IF(IOKENT.NE.0) GO TO 1 +C THIS RETURNS THE FOLLOWING: +C ILEFTS POSITION IN CONSENSUS OF LEFT END OF MATCHING CONTIGS +C ILC LENGTHS OF MATCHING CONTIGS +C IPOSC POSITION OF MATCH RELATIVE TO CONTIG +C IPOSG POSITION OF MATCH RELATIVE TO NEW GEL +C ISENSE SENSE OF NEW GEL +C LLINO LEFT GEL NUMBER IN MATCHING CONTIGS +C IMATC THE NUMBER OF MATCHING CONTIGS (>2 IS ERROR!) +C IFCOMP ERROR FLAG FOR COMPARISON (COMPARISON ARRAYS OVERFLOWED) + IF(IFCOMP.NE.0)GO TO 800 + CALL SQCOPY(SEQ3,SEQ2,IDIM2) + IF(IMATC.GT.0)GO TO 200 +C +C NO OVERLAP NEW CONTIG +C +C ITYPE 0 = NO OVERLAP +C ISENSE 1 = SAME SENSE AS ARCHIVE + IF(IFAIL(1).NE.0) GO TO 800 + ITYPE(1)=0 + ISENSE(1)=1 + IDOUT(1)=MAXGEL + WRITE(IDEV,1015) +1015 FORMAT(' New gel reading does not overlap: start a new contig') + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ2,NAMARC,X,ITYPE,ISENSE,SEQC2(1,1),ITOTPC(1), + +IDIM2,IDOUT(1),LLINO,LINCON,IFAIL,IDBSIZ,IDEV, + +IDEV1,IDEV2,IDEV3,IDEVT,IDEVC,IDEV4,MAXGEL) + IF(IFAIL(1).NE.0)GO TO 800 + IEMPTY=0 + IDIM1=IDIM1+1 + IF((IDIM1+19+IDIM2).GT.MAXSEQ)THEN + WRITE(IDEV,1021)MAXSEQ +1021 FORMAT(' Database maximum consensus length (',I6,') exceeded') + GO TO 900 + END IF + CALL ADDTIT(SEQ1(IDIM1),NAMPRO,NGELS,IDIM1) + CALL MSTLKL(SEQ2,IDIM2) + CALL SQCOPY(SEQ2,SEQ1(IDIM1),IDIM2) + IDIM1=IDIM1+IDIM2-1 + JNGEL = JNGEL + 1 + GO TO 1 +C +C +C +200 CONTINUE +C +C +C OVERLAP SO TRY TO ALIGN THE SEQUENCES +C +C + DO 100 I=1,IMATC + N=IDBSIZ-NCONTS + DO 99 J=N,IDBSIZ-1 + IF(LNBR(J).NE.LLINO(I))GO TO 99 + LINCON(I)=J + GO TO 100 +99 CONTINUE + WRITE(IDEV,10077)LLINO(I) +10077 FORMAT(' Contig line for contig',I6,' not found!') + GO TO 800 +100 CONTINUE +C + IF((IMATC.EQ.2).AND.(ANSJOK.EQ.0))GO TO 400 +C +C +C SINGLE OVERLAP +C +C +C + WRITE(IDEV,1014)LLINO(1) +1014 FORMAT(' New gel reading overlaps contig',I6) + IF(ITOTPG(1).GT.0) CALL CCTA(SEQG2(1,1),IDIM22(1)) + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQG2(1,1),NAMARC,JOINT(1),ITYPE(1),ISENSE(1), + +SEQC2(1,1), + +ITOTPC(1),IDIM22(1),IDOUT(1),LLINO(1),LINCON(1), + +IFAIL(1),IDBSIZ,IDEV,IDEV1,IDEV2,IDEV3,IDEVT,IDEVC,IDEV4,MAXGEL) + IF(IFAIL(1).NE.0)GO TO 800 + CALL DELCON(SEQ1,ILEFTS(1),ILC(1),IDIM1) + LREG=1 + RREG=RELPG(LINCON(1)) + IGELC=LNBR(LINCON(1)) + ANS=1 + CALL ACONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,MAXSEQ,SEQ2,IDBSIZ,IDIM1,ANS,IGELC,LREG,RREG,TEMP3, + +ECHRSZ,MAXGL2,IDEV,IDEV2,IFAIL(1),MAXGEL,IDM,PERCD) + IF(IFAIL(1).NE.0)GO TO 900 + JNGEL = JNGEL + 1 + IF(KFAIL.NE.0) GO TO 800 + GO TO 1 +C +C +C DOUBLE OVERLAP +C +C +400 CONTINUE + WRITE(IDEV,1013)LLINO +1013 FORMAT(' Overlap between contigs',I6,' and',I6) + IF(ANSJOK.NE.0)GO TO 800 + IF(LLINO(1).EQ.LLINO(2))THEN + WRITE(IDEV,*)' Trying to form loop in contig',LLINO(1) + WRITE(IDEV,*)' Gel not entered' + GO TO 800 + END IF + CALL AJOIN3(RELPG,IDBSIZ,LINCON,ITYPE,ISENSE,JOINT, + +IDIM22,KLASS,IOVER,IDEV,PL,PR) + IF(IOVER.GT.MAXOVR)THEN + WRITE(IDEV,*)' Overlap too large: entry only' + IFAIL(2)=1 + GO TO 600 + END IF +C WHICH CONTIG IS LEFTMOST? + LMOST=1 + RMOST=2 + IF(PL(1).GT.PL(2))THEN + LMOST=2 + RMOST=1 + END IF +C SAVE LENGTH OF RMOST CONTIG FOR DELETION STEP LATER + ILCR=ILC(RMOST) + IF(ITOTPG(LMOST).GT.0) CALL CCTA(SEQG2(1,LMOST),IDIM22(LMOST)) + WRITE(IDEV,1012)LLINO(LMOST) +1012 FORMAT(' Entering the new gel reading into contig',I6) + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQG2(1,LMOST),NAMARC,JOINT(LMOST),ITYPE(LMOST), + +ISENSE(LMOST),SEQC2(1,LMOST),ITOTPC(LMOST), + +IDIM22(LMOST),IDOUT(LMOST),LLINO(LMOST),LINCON(LMOST), + +IFAIL(LMOST),IDBSIZ,IDEV,IDEV1,IDEV2,IDEV3,IDEVT,IDEVC,IDEV4, + +MAXGEL) + IF(IFAIL(LMOST).NE.0)GO TO 800 + JNGEL = JNGEL + 1 + IF(ITYPE(LMOST).EQ.1)LLINO(LMOST)=NGELS + IF(ILEFTS(LMOST).LT.ILEFTS(RMOST))THEN + ILEFTS(RMOST)=ILEFTS(RMOST)-ILC(LMOST)-20 + END IF + CALL DELCON(SEQ1,ILEFTS(LMOST),ILC(LMOST),IDIM1) + LREG=1 + RREG=RELPG(LINCON(LMOST)) + IGELC=LNBR(LINCON(LMOST)) + ANS=1 + CALL ACONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,MAXSEQ,SEQ2,IDBSIZ,IDIM1,ANS,IGELC,LREG,RREG,TEMP3, + +ECHRSZ,MAXGL2,IDEV,IDEV2,IFAIL(1),MAXGEL,IDM,PERCD) + IF(IFAIL(1).NE.0)GO TO 900 + ILC(LMOST)=RREG + ILEFTS(LMOST)=IDIM1-RREG+1 + DO 500 I=1,2 + IF(ISENSE(I).EQ.-1)THEN + CALL CMPLMT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON(I), + + LLINO(I),SEQ2,IDBSIZ,IDEV,IDEV1,IDEV2,MAXGEL) + CALL SQREV(SEQ1(ILEFTS(I)),ILC(I)) + CALL SQCOM(SEQ1(ILEFTS(I)),ILC(I)) + KT=IDIM1 + CALL ADDTIT(SEQ1((ILEFTS(I)-20)),NAMPRO,LNBR(LINCON(I)),KT) + END IF +500 CONTINUE +C NEED TO KNOW POSITION OF OVERLAP RELATIVE TO CONTIG, TO CONSENSUS +C WHICH BITS TO SEND TO ALIGNMENT ROUTINES +C SET UP FOR ALINE (NOTE RMOST IS EQUIVALENT TO THE GEL READING AND +C SO IS SLID ALONG THE LMOST CONTIG. THE SECTION SENT TO ALINE MUST +C BE OF LENGTH < MAXGEL-2*MAX(MAXPC,MAXPG) +C IT MUST START AT POSITION 1 IN THE RMOST CONTIG AND EXTEND + IPOSC(LMOST)=PL(RMOST)+RELPG(NGELS)-1 + ILCT = RELPG(LINCON(LMOST)) - RELPG(NGELS) - PL(RMOST) + 2 + ILC(RMOST)=MIN(ILCT,ILC(RMOST)) + IPOSC(RMOST)=1 + IDOUT(LMOST)=MAXGEL + IDOUT(RMOST)=MAXGEL + IDSAV=MAXSAV +C ON INPUT TO ALINE ILC(RMOST) CONTAINS THE OVERLAP LENGTH +C ON OUTPUT IT CONTAINS THE LENGTH OF THE ALIGNED SECTION (IE INCLUDING +C PADS) + WRITE(IDEV,1009) +1009 FORMAT(' Trying to align the two contigs') + CALL ALINE(SEQ1(ILEFTS(LMOST)),SEQ1(ILEFTS(RMOST)), + +SEQC2(1,RMOST),SEQC2(1,LMOST),SAV1,SAV2,SAV3,IDSAV, + +ILC(LMOST),ILC(RMOST),IDOUT(LMOST),IPOSC(LMOST),IPOSC(RMOST), + +MINSLI,JOINT(LMOST),ITOTPC(LMOST),ITOTPC(RMOST),IFAIL(1), + +ITYPE(1),MAXPC,MAXPC,PERMAX,IDEV,SEQ4,MAXGEL,Z) +C SEQC2(1,LMOST) NOW CONTAINS THE ALIGNED SECTION OF THE LMOST CONTIG +C SEQC2(1,RMOST) NOW CONTAINS THE ALIGNED SECTION OF THE RMOST CONTIG +C ILC(RMOST) IS NOW THE LENGTH OF ALIGNED SECTION OF THE RMOST CONTIG +C IDOUT(LMOST) IS NOW THE LENGTH OF ALIGNED SECTION OF THE LMOST CONTIG +C JOINT(LMOST) IS THE POSITION OF THE JOIN RLETIVE TO THE LMOST CONTIG +C ITYPE IS TYPE OF OVERLAP (-1 = RIGHT END OR INTERNAL, 1 = LEFT END) +C NB SHOULD ALWAYS BE -1 +C IF THIS HAS BEEN DONE OK WE CAN EDIT THE TWO CONTIGS THEN JOIN + IF(IFAIL(1).NE.0)THEN + WRITE(IDEV,*)' Failed to align the two overlapping contigs' + GO TO 800 + END IF + IF(ITOTPC(LMOST).GT.0)THEN + WRITE(IDEV,1017)LLINO(LMOST) +1017 FORMAT(' Editing contig',I6) + CALL ABEDIN(RELPG,LNGTHG,LNBR,RNBR, + + NGELS,NCONTS,SEQ3,LINCON(LMOST),JOINT(LMOST),SEQC2(1,LMOST), + + ITOTPC(LMOST),IDOUT(LMOST),IDBSIZ,IDEV,IDEV1,IDEV2, + + MAXGEL) + END IF + JOINT(RMOST)=1 + IDOUT(RMOST)=ILC(RMOST) + IF(ITOTPC(RMOST).GT.0)THEN + WRITE(IDEV,1017)LLINO(RMOST) + CALL ABEDIN(RELPG,LNGTHG,LNBR,RNBR, + + NGELS,NCONTS,SEQ3,LINCON(RMOST),JOINT(RMOST),SEQC2(1,RMOST), + + ITOTPC(RMOST),IDOUT(RMOST),IDBSIZ,IDEV,IDEV1,IDEV2, + + MAXGEL) + END IF + ILC(RMOST)=ILCR + LTL=LNBR(LINCON(LMOST)) + LTR=LNBR(LINCON(RMOST)) + WRITE(IDEV,1018)LNBR(LINCON(LMOST)),LNBR(LINCON(RMOST)) +1018 FORMAT(' Completing the join between contigs',I6,' and',I6) + CALL AJOIN2(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +JOINT(LMOST),LTL,LTR,LINCON(LMOST),LINCON(RMOST),IDEV1) + LLINO(1)=LTL + IF(ILEFTS(LMOST).GT.ILEFTS(RMOST))THEN + CALL DELCON(SEQ1,ILEFTS(LMOST),ILC(LMOST),IDIM1) + CALL DELCON(SEQ1,ILEFTS(RMOST),ILC(RMOST),IDIM1) + END IF + IF(ILEFTS(RMOST).GE.ILEFTS(LMOST))THEN + CALL DELCON(SEQ1,ILEFTS(RMOST),ILC(RMOST),IDIM1) + CALL DELCON(SEQ1,ILEFTS(LMOST),ILC(LMOST),IDIM1) + END IF + LREG=1 + RREG=JOINT(LMOST) + IGELC=LLINO(1) + ANS=1 + CALL ACONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,MAXSEQ,SEQ2,IDBSIZ,IDIM1,ANS,IGELC,LREG,RREG,TEMP3, + +ECHRSZ,MAXGL2,IDEV,IDEV2,IFAIL(1),MAXGEL,IDM,PERCD) + IF(IFAIL(1).NE.0)GO TO 900 +C CALL FMTDB(SEQ1,IDIM1,1,IDIM1,60,IDEV) + JNJOIN = JNJOIN + 1 + IF(KFAIL.NE.0) GO TO 800 + GO TO 1 +C +C +C JOINS THAT FAIL INITIAL ALIGNMENT CRITERIA +C +C +600 CONTINUE +C COME HERE FOR JOINS THAT FAIL: WE MIGHT BE ABLE TO ENTER THE GEL +C INTO A SINGLE CONTIG + IGOOD=0 + IF(IFAIL(1).EQ.0)IGOOD=1 + IF(IFAIL(2).EQ.0)IGOOD=2 + IF(IGOOD.EQ.0)GO TO 800 + IF(ITOTPG(IGOOD).GT.0) CALL CCTA(SEQG2(1,IGOOD),IDIM22(IGOOD)) + WRITE(IDEV,1012)LLINO(IGOOD) + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQG2(1,IGOOD),NAMARC,JOINT(IGOOD),ITYPE(IGOOD), + +ISENSE(IGOOD),SEQC2(1,IGOOD),ITOTPC(IGOOD), + +IDIM22(IGOOD),IDOUT(IGOOD),LLINO(IGOOD),LINCON(IGOOD), + +IFAIL(IGOOD),IDBSIZ,IDEV,IDEV1,IDEV2,IDEV3,IDEVT,IDEVC,IDEV4, + +MAXGEL) + IF(IFAIL(IGOOD).NE.0)GO TO 800 + JNGEL = JNGEL + 1 + CALL DELCON(SEQ1,ILEFTS(IGOOD),ILC(IGOOD),IDIM1) + ANS=1 + IGELC=LNBR(LINCON(IGOOD)) + LREG=1 + RREG=RELPG(LINCON(IGOOD)) + CALL ACONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,MAXSEQ,SEQ2,IDBSIZ,IDIM1,ANS,IGELC,LREG,RREG,TEMP3, + +ECHRSZ,MAXGL2,IDEV,IDEV2,IFAIL(1),MAXGEL,IDM,PERCD) + IF(IFAIL(1).NE.0)GO TO 900 + WRITE(IDEV,1020)LLINO,LLINO(IGOOD) +1020 FORMAT(' Could not join contigs',I4,' and',I4,' but the gel', + +' has been entered into contig',I4,/, + +' If required do the join manually.') +C +C +C ALL FAILURES +C +C +C +800 CONTINUE + WRITE(IDEV,10888) +10888 FORMAT(' Failed gel name written to error file') + WRITE(IDEV8,1002)NAMARC + GO TO 1 +900 CONTINUE + WRITE(KBOUT,*)'Batch finished' + WRITE(KBOUT,*)JGEL,' sequences processed' + WRITE(KBOUT,*)JNGEL,' sequences entered into database' + WRITE(KBOUT,*)JNJOIN,' joins made' + RETURN + END + SUBROUTINE DBAUTP(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ2,NAMARC,JOINT,ITYPE,ISENSE,SEQC2,ITOTPC, + +IDIM2,IDOUT,LLINO,LINCON,IFAIL,IDBSIZ,MAXDB,IDEV, + +IDEV1,IDEV2,IDEV3,IDEVT,IDEVC,IDEV4,MAXGEL,IMATC,IEMPTY,IOPT) + INTEGER RELPG(MAXDB) + INTEGER LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER SEQ2(MAXGEL),SEQC2(MAXGEL) + CHARACTER NAMARC*(*) +C deals with entering all readings into contig 1 (IOPT=2) +C or all readings into new contigs (IOPT=3) + IF(IOPT.EQ.2) THEN + IF(IMATC.EQ.0) THEN + ITYPE=0 + ISENSE=1 + IDOUT=MAXGEL + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + SEQ2,NAMARC,JOINT,ITYPE,ISENSE,SEQC2,ITOTPC, + + IDIM2,IDOUT,LLINO,LINCON,IFAIL,IDBSIZ,IDEV, + + IDEV1,IDEV2,IDEV3,IDEVT,IDEVC,IDEV4,MAXGEL) + IF(IFAIL.NE.0) RETURN + IEMPTY=0 + IMATC = 1 + ELSE + ITYPE= - 1 + ISENSE=1 + JOINT = 1 + LLINO = 1 + LINCON = IDBSIZ - 1 + ITOTPC = 0 + IDOUT=MAXGEL + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + SEQ2,NAMARC,JOINT,ITYPE,ISENSE,SEQC2,ITOTPC, + + IDIM2,IDOUT,LLINO,LINCON,IFAIL,IDBSIZ,IDEV, + + IDEV1,IDEV2,IDEV3,IDEVT,IDEVT,IDEV4,MAXGEL) + IF(IFAIL.NE.0) RETURN + END IF + ELSE IF(IOPT.EQ.3) THEN + ITYPE=0 + ISENSE=1 + IDOUT=MAXGEL + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + SEQ2,NAMARC,JOINT,ITYPE,ISENSE,SEQC2,ITOTPC, + + IDIM2,IDOUT,LLINO,LINCON,IFAIL,IDBSIZ,IDEV, + + IDEV1,IDEV2,IDEV3,IDEVT,IDEVC,IDEV4,MAXGEL) + IF(IFAIL.NE.0) RETURN + END IF + END + SUBROUTINE DBCOPY(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,GEL, + +NAMPRO,IDEV,IDBSIZ,IERR,KBIN,KBOUT,IDEVR,IDEVW,IDEVN, + +IDEVT,IDEVC, + +IHELPS,IHELPE,FILEH,IDEVH,MAXGEL,MAXDB,IDM) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER NAMPRO*(*),NAMARC*10,GEL(MAXGEL) + CHARACTER V2,V1 + INTEGER IWORD,DELDB + PARAMETER (IWORD=4) + PARAMETER (MAXPRM = 21) + CHARACTER PERR(2)*(MAXPRM) + EXTERNAL DELDB + IERR=1 +1 CONTINUE + L = 1 + V1='1' + CALL GTSTR('Make version',V1,V2,L,KBOUT,KBIN,INFLAG) + CALL CCASE(V2,1) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(L.LT.1) V2 = V1 + MN = NGELS + NCONTS + 1 + MX = MAXDB + NEWSIZ = IDBSIZ + CALL GETINT(MN,MX,NEWSIZ, + +'New database size', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL BUSY(KBOUT) + NEWSIZ = IVAL +C WHERE SHOULD CHARS BE CHANGED ? + LLL = INDEX(NAMPRO,'.') + 1 + NAMPRO(LLL:)='RL'//V2 + CALL OPENRS(IDEV,NAMPRO,IOK,4,3) + IF(IOK.NE.0) THEN +C problem opening file + IF(IOK.EQ.2) THEN + CALL ERROM(KBOUT,'File already exists') + PERR(1) = 'Retype version number' + PERR(2) = 'Replace database' + IDO = 1 + CALL RADION('Select action',PERR,2,IDO, + + IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(IDO.LT.1) RETURN + IF(IDO.EQ.1) GO TO 1 + IF(IDO.EQ.2) THEN + IOK = DELDB(NAMPRO,V2,IDEV,MAXGEL) + IF(IOK.EQ.0) THEN + LLL = INDEX(NAMPRO,'.') + 1 + NAMPRO(LLL:)='RL'//V2 + CALL OPENRS(IDEV,NAMPRO,IOK,4,3) + IF(IOK.EQ.0) GO TO 2 + ELSE + CALL ERROM(KBOUT,'File delete failed') + END IF + END IF + END IF + GO TO 100 + END IF + 2 CONTINUE + CALL WRITER(IDEV,NEWSIZ,NGELS,NCONTS,NGELS,NCONTS) + DO 10 I=1,NGELS + CALL WRITER(IDEV,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) +10 CONTINUE + M=NEWSIZ-NCONTS + N=IDBSIZ-NCONTS + DO 15 I=N,IDBSIZ-1 + CALL WRITER(IDEV,M,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + M=M+1 +15 CONTINUE + CLOSE(UNIT=IDEV) +C DO SEQUENCES + NAMPRO(LLL:)='SQ'//V2 + IREC=MAXGEL/IWORD + IF(MOD(MAXGEL,IWORD).NE.0)IREC=IREC+1 + CALL OPENRS(IDEV,NAMPRO,IOK,IREC,3) + IF(IOK.NE.0)GO TO 100 +C +C MAKE SURE FILE IS NEWSIZ LINES LONG! + CALL WRITEW(IDEV,NEWSIZ,GEL,MAXGEL) + DO 20 I=1,NGELS + CALL READW(IDEVW,I,GEL,MAXGEL) + CALL WRITEW(IDEV,I,GEL,MAXGEL) +20 CONTINUE + CLOSE(UNIT=IDEV) +C DO ARCHIVE NAMES + NAMPRO(LLL:)='AR'//V2 + CALL OPENRS(IDEV,NAMPRO,IOK,3,3) + IF(IOK.NE.0)GO TO 100 + DO 30 I=1,NGELS + CALL READN(IDEVN,I,NAMARC) + CALL WRITEN(IDEV,I,NAMARC) +30 CONTINUE +C MAKE SURE FILE IS 1000 LINES LONG +C ******** PROBLEMS HERE WRITING INTEGER TO NAMES FILE + WRITE(IDEV,REC=MAXDB)NEWSIZ,MAXGEL,IDM + CLOSE(UNIT=IDEV) +C IF(IDEVRD.GT.0) THEN +C NAMPRO(LLL:) = 'RD'//V2 +C CALL OPENRS(IDEV,NAMPRO,IOK,7,3) +C IF(IOK.NE.0) GO TO 100 +C CALL COPYRD(IDEVRD,IDEV,NGELS,NEWSIZ,IOK) +C IF(IOK.NE.0) GO TO 100 +C END IF +C DO TAGS + COMMENTS + IF(IDEVT.GT.0.AND.IDEVC.GT.0) THEN + NAMPRO(LLL:)='TG'//V2 + CALL OPENRS(IDEV,NAMPRO,IOK,5,3) + IF(IOK.NE.0)GO TO 100 + CALL COPYTG(IDEVT,IDEV,IOK,IDBSIZ,NEWSIZ,NGELS) + NAMPRO(LLL:)='CC'//V2 +C COMMENT_LENGTH: 11 = (40 + long)/long + CALL OPENRS(IDEV,NAMPRO,IOK,11,3) + IF(IOK.NE.0)GO TO 100 + CALL COPYCC(IDEVC,IDEV,IOK) + ENDIF + IERR=0 + RETURN +100 CONTINUE + WRITE(KBOUT,9999) +9999 FORMAT(' Error opening new database, copy aborted') + RETURN + END + INTEGER FUNCTION DELDB(NAMPRO,VERSN,IDEV,MAXGEL) + CHARACTER NAMPRO*(*),VERSN + INTEGER DELF + EXTERNAL DELF +C +C delete an xdap database +C +C assume relationships are 4 words, names are 3 and seqs are maxgel +C all recls in BYTES +C + DELDB = 1 + LLL = INDEX(NAMPRO,'.') + 1 + NAMPRO(LLL:)='RL'//VERSN + IF(DELF(NAMPRO,IDEV,16,4).NE.0) RETURN + NAMPRO(LLL:)='AR'//VERSN + IF(DELF(NAMPRO,IDEV,12,4).NE.0) RETURN + NAMPRO(LLL:)='SQ'//VERSN + IF(DELF(NAMPRO,IDEV,MAXGEL,4).NE.0) RETURN + NAMPRO(LLL:)='CC'//VERSN +C COMMENT_LENGTH: 11 = (40 + long)/long + IF(DELF(NAMPRO,IDEV,44,4).NE.0) RETURN + NAMPRO(LLL:)='TG'//VERSN + IF(DELF(NAMPRO,IDEV,20,4).NE.0) RETURN + DELDB = 0 + END + SUBROUTINE DBFIX(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,GEL2,IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW,IDEVN, + +IHELPS,IHELPE,IHELP1,IHELP2,FILEH,IDEVH,MAXGEL,IDEVT,IDEVC) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN +C 12-12-90 Added function to change raw data parameter file +C and changed menu routines accordingly + INTEGER RELPG(IDBSIZ),X + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + CHARACTER NAME*10,NEWNAM*10 + INTEGER L,M,N + CHARACTER GEL2(MAXGEL) + PARAMETER (MAXPRM = 32) + CHARACTER PROMPT(9)*(MAXPRM) + INTEGER GCLIN,CHAINL + EXTERNAL GCLIN,CHAINL + WRITE(KBOUT,1000) +1000 FORMAT( + +' Warning:', + +' make a copy first, and check logical consistency after use') +10 CONTINUE +C +C SELECT OPTION +C CALL BELL(1,KBOUT) +C DBMENU now defunct for bap - so we use RADION instead +C CALL DBMENU(4,NOPT,IHELPS,IHELPE,FILEH,IDEVH, +C +KBIN,KBOUT) + PROMPT(1) = 'Line change' + PROMPT(2) = 'Edit gel reading' + PROMPT(3) = 'Delete contig line' + PROMPT(4) = 'Shift' + PROMPT(5) = 'Move gel reading' + PROMPT(6) = 'Rename gel reading' + PROMPT(7) = 'Break a contig' + PROMPT(8) = 'Remove a gel reading' + PROMPT(9) = 'Alter raw data parameters' + NOPT = 1 + CALL RADION('Alter relationships', PROMPT, 9, NOPT, IHELPS, + + IHELPE, FILEH, IDEVH, KBIN, KBOUT) + IF(NOPT.LT.1)RETURN + IF(NOPT.EQ.1)THEN +C LINE CHANGE + MN = 0 + MX = IDBSIZ + LNO = 0 + CALL GETINT(MN,MX,LNO, + + 'Number of line to change', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + IF(IVAL.EQ.0) GO TO 10 + LNO = IVAL + IF(LNO.EQ.IDBSIZ)GO TO 19 + WRITE(KBOUT,*)'Current line' + WRITE(KBOUT,1001)RELPG(LNO),LNGTHG(LNO),LNBR(LNO),RNBR(LNO) +1001 FORMAT(' ',4I6) + MN = 0 + MX = 99999 + X = RELPG(LNO) + CALL GETINT(MN,MX,X, + + 'Relative position', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + X = IVAL + MN = -MAXGEL + MX = 99999 + L = LNGTHG(LNO) + CALL GETINT(MN,MX,L, + + 'Length', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + L = IVAL + MN = 0 + MX = IDBSIZ + M = LNBR(LNO) + CALL GETINT(MN,MX,M, + + 'Left neighbour', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + M = IVAL + MN = 0 + MX = IDBSIZ + N = RNBR(LNO) + CALL GETINT(MN,MX,N, + + 'Right neighbour', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + N = IVAL + CALL WRITER(IDEVR,LNO,X,L,M,N) + RELPG(LNO)=X + LNGTHG(LNO)=L + LNBR(LNO)=M + RNBR(LNO)=N + GO TO 10 +19 CONTINUE +C NCONTS NGELS LINES + MN = 0 + MX = IDBSIZ + LL = NGELS + CALL GETINT(MN,MX,LL, + + 'Number of gel readings', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + LL = IVAL + MN = 0 + MX = IDBSIZ + MM = NCONTS + CALL GETINT(MN,MX,MM, + + 'Number of contigs', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + MM = IVAL + CALL WRITER(IDEVR,IDBSIZ,LL,MM,LL,MM) + NGELS=LL + NCONTS=MM + GO TO 10 + END IF +C +C + IF(NOPT.EQ.4)THEN + MN = 0 + MX = NGELS + LNO = 0 + CALL GETINT(MN,MX,LNO, + + 'Number of first gel reading to shift', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + IF(IVAL.LT.1) GO TO 10 + LNO = IVAL + I = CHAINL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,LNO) + IF(I.EQ.0)THEN + WRITE(KBOUT,*) + + 'Problem with this gel reading. Check logical consistency' + WRITE(KBOUT,*)'of database. Shift not done' + GO TO 10 + END IF + NCONTO = GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,I) + IF(NCONTO.EQ.0)THEN + WRITE(KBOUT,*) + + 'No contig line for this contig. Check logical' + WRITE(KBOUT,*)'consistency of database. Shift not done' + GO TO 10 + END IF + MN = 1 - RELPG(LNO) + MX = RELPG(NCONTO) - RELPG(LNO) + X = MN + CALL GETINT(MN,MX,X, + + 'Distance to shift', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + X = IVAL + CALL SHIFTC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDEVR, + + IDBSIZ,LNO,NCONTO,X) + WRITE(KBOUT,*)'Shift complete' + GO TO 10 + END IF +C + IF(NOPT.EQ.2)THEN + MN = 0 + MX = NGELS + LNO = 0 + CALL GETINT(MN,MX,LNO, + + 'Number of gel reading to edit', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + IF(IVAL.LT.1) GO TO 10 + LNO = IVAL + IDIM=ABS(LNGTHG(LNO)) +C ALLOW EDITING TO BE RELATIVE TO CONTIG POSITION + ISTART=RELPG(LNO) + CALL READW(IDEVW,LNO,GEL,MAXGEL) +C @jkb@ +C CALL EDITGL(GEL,GEL2,IDIM,KBIN,KBOUT,MAXGEL, +C + IHELP1,IHELP2,FILEH,IDEVH,ISTART) + CALL WRITEW(IDEVW,LNO,GEL,MAXGEL) + LNGTHG(LNO)=SIGN(IDIM,LNGTHG(LNO)) + CALL WRITER(IDEVR,LNO,RELPG(LNO),LNGTHG(LNO), + + LNBR(LNO),RNBR(LNO)) + GO TO 10 + END IF + IF(NOPT.EQ.3)THEN + MN=IDBSIZ-NCONTS + MX = IDBSIZ - 1 + LNO = 0 + CALL GETINT(MN,MX,LNO, + + 'Number of contig line to delete', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + LNO = IVAL + CALL REMCNL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + LNO,IDEVR) + GO TO 10 + END IF + IF(NOPT.EQ.6)THEN + MN = 0 + MX = NGELS + LNO = 0 + CALL GETINT(MN,MX,LNO, + + 'Number of gel reading to rename', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + IF(IVAL.LT.1) GO TO 10 + LNO = IVAL + CALL READN(IDEVN,LNO,NAME) + L = 10 + CALL GTSTR('name for gel reading', + + NAME,NEWNAM,L,KBOUT,KBIN,INFLAG) + IF(L.GT.0)CALL WRITEN(IDEVN,LNO,NEWNAM) + GO TO 10 + END IF + IF(NOPT.EQ.5)THEN + MN = 0 + MX = NGELS + IFROM = 0 + CALL GETINT(MN,MX,IFROM, + + 'Number of gel reading to move', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + IF(IVAL.LT.1) GO TO 10 + IFROM = IVAL + MN = 0 + MX = NGELS + ITO = 0 + CALL GETINT(MN,MX,ITO, + + 'New number for gel reading', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + IF(IVAL.LT.1) GO TO 10 + ITO = IVAL + CALL MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + GEL,IFROM,ITO,IDEVR,IDEVW,IDEVN,MAXGEL,KBOUT) + GO TO 10 + END IF + IF(NOPT.EQ.7)THEN + CALL BREAKC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW,IDEVN, + + IHELPS,IHELPE,IHELP1,IHELP2,FILEH,IDEVH,IOK) + GO TO 10 + END IF + IF(NOPT.EQ.8) THEN + CALL REMGD(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + KBIN,KBOUT,GEL,MAXGEL,IDEVR,IDEVW,IDEVN, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + GO TO 10 + END IF + IF(NOPT.EQ.9) THEN + CALL FIXRD(IDEVT,IDEVC,IDBSIZ,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH) + GO TO 10 + END IF + GO TO 10 + END + SUBROUTINE REMGD(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +KBIN,KBOUT,GEL,MAXGEL,IDEVR,IDEVW,IDEVN, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER HELPF*(*),GEL(MAXGEL) + INTEGER REMME,GCLIN,CHAINL + EXTERNAL GCLIN,CHAINL +C assumes db is logical consistent + WRITE(KBOUT,*)'Remove reading from database' + REMME = NGELS + CALL GETINT(1,NGELS,REMME, + +'Number of reading to remove', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + REMME = IVAL + I = CHAINL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,REMME) + ICONT = GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ,I) + IF(ICONT.EQ.0) THEN + WRITE(KBOUT,*)'No contig line for this reading' + IOK = 1 + RETURN + END IF + CALL REMGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +REMME,ICONT,KBOUT,GEL,MAXGEL,IDEVR,IDEVW,IDEVN) + END + SUBROUTINE REMGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +REMME,ICONT,KBOUT,GEL,MAXGEL,IDEVR,IDEVW,IDEVN) +C Routine to remove a reading from a database +C Cases: 1 left end +C 2 right end +C 3 internal and dispensible +C 4 internal and indispensible +C if 1 change contig lnbr, contig length, lnbr of rnbr of remme, relpgs +C if 2 change contig rnbr, contig length, rnbr of lnbr of remme +C if 3 change contig length, lnbr of rnbr of remme rnbr of lnbr of remme +C if 4 need to break contig, then as for 1 +C if 1 and 2 then also remove contig line +C for all cases move gel ngels to remme (if remme/=ngels) +C and update line idbsiz +C + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER REMME,CLEN + LOGICAL LEFTE,RIGHTE,DISPEN + CHARACTER GEL(MAXGEL) + EXTERNAL CLEN + LEFTE = .FALSE. + RIGHTE = .FALSE. + DISPEN = .FALSE. +C +C Left end ? +C + IF(LNBR(REMME).EQ.0) LEFTE = .TRUE. +C +C Right end ? +C + IF(RNBR(REMME).EQ.0) RIGHTE = .TRUE. +C +C If both true remove the contig line, then overwrite the gel +C + IF(LEFTE.AND.RIGHTE) THEN + WRITE(KBOUT,*)'Removing reading and contig' + IFROM = NGELS + NGELS = NGELS - 1 + CALL REMCNL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + ICONT,IDEVR) + IF(REMME.NE.IFROM) THEN + WRITE(KBOUT,*)'Renumbering reading',IFROM,' to',REMME + CALL MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,GEL,IFROM,REMME,IDEVR,IDEVW,IDEVN,MAXGEL,KBOUT) + END IF + ELSE IF(LEFTE) THEN + WRITE(KBOUT,*)'Removing reading from left end of contig' + LNBR(ICONT) = RNBR(REMME) + I = 1 - RELPG(RNBR(REMME)) + WRITE(KBOUT,*)'Shifting readings in contig by distance=',I + CALL SHIFTC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDEVR, + + IDBSIZ,RNBR(REMME),ICONT,I) + I = LNBR(ICONT) + LNBR(I) = 0 + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + IFROM = NGELS + IF(REMME.NE.IFROM) THEN + WRITE(KBOUT,*)'Renumbering reading',IFROM,' to',REMME + CALL MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,GEL,IFROM,REMME,IDEVR,IDEVW,IDEVN,MAXGEL,KBOUT) + END IF + NGELS = NGELS - 1 + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + ELSE IF(RIGHTE) THEN + WRITE(KBOUT,*)'Removing reading from right end of contig' + RNBR(ICONT) = LNBR(REMME) + I = RNBR(ICONT) + RNBR(I) = 0 + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + RELPG(ICONT) = CLEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,LNBR(ICONT)) + CALL WRITER(IDEVR,ICONT,RELPG(ICONT),LNGTHG(ICONT), + + LNBR(ICONT),RNBR(ICONT)) + IFROM = NGELS + IF(REMME.NE.IFROM) THEN + WRITE(KBOUT,*)'Renumbering reading',IFROM,' to',REMME + CALL MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,GEL,IFROM,REMME,IDEVR,IDEVW,IDEVN,MAXGEL,KBOUT) + END IF + NGELS = NGELS - 1 + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + ELSE +C Is remme indispensible ? + NSTART = RELPG(RNBR(REMME)) + I = REMME +10 CONTINUE + I = LNBR(I) + IF(I.NE.0) THEN + IF((RELPG(I)+ABS(LNGTHG(I))-1).LT.NSTART) GO TO 10 + DISPEN = .TRUE. + END IF + IF(DISPEN) THEN + WRITE(KBOUT,*) + + 'Removing dispensible reading from middle of contig' + I = LNBR(REMME) + RNBR(I) = RNBR(REMME) + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + I = RNBR(REMME) + LNBR(I) = LNBR(REMME) + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + IFROM = NGELS + IF(REMME.NE.IFROM) THEN + WRITE(KBOUT,*)'Renumbering reading',IFROM,' to',REMME + CALL MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,GEL,IFROM,REMME,IDEVR,IDEVW,IDEVN, + + MAXGEL,KBOUT) + END IF + NGELS = NGELS - 1 + RELPG(ICONT) = CLEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,LNBR(ICONT)) + CALL WRITER(IDEVR,ICONT,RELPG(ICONT),LNGTHG(ICONT), + + LNBR(ICONT),RNBR(ICONT)) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + ELSE + WRITE(KBOUT,*) + + 'Removing indispensible reading from middle of contig' + WRITE(KBOUT,*)'So breaking contig first' + IR = REMME + IL = LNBR(REMME) + ILO = LNBR(ICONT) + NCONTO = ICONT + NCONTR = IDBSIZ - NCONTS - 1 + CALL CBREAK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + KBOUT,IDEVR,IDEVW,IDEVN,IR,IL,ILO,NCONTO,NCONTR,IOK) + IF(IOK.NE.0) RETURN + WRITE(KBOUT,*)'Removing reading from left end of contig' + ICONT = IDBSIZ - NCONTS + LNBR(ICONT) = RNBR(REMME) + I = 1 - RELPG(RNBR(REMME)) + WRITE(KBOUT,*)'Shifting readings in contig by distance=',I + CALL SHIFTC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDEVR, + + IDBSIZ,RNBR(REMME),ICONT,I) + I = LNBR(ICONT) + LNBR(I) = 0 + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + IFROM = NGELS + IF(REMME.NE.IFROM) THEN + WRITE(KBOUT,*)'Renumbering reading',IFROM,' to',REMME + CALL MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,GEL,IFROM,REMME,IDEVR,IDEVW,IDEVN, + + MAXGEL,KBOUT) + END IF + NGELS = NGELS - 1 + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + END IF + END IF + END + SUBROUTINE REMCNL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +REMME,IDEVR) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER REMME +C Routine to remove a contig line from a db +C Loop deals with case of remove top contig +C Move down all lines from above + DO 10 I = REMME,IDBSIZ-NCONTS+1,-1 + RELPG(I) = RELPG(I-1) + LNGTHG(I) = LNGTHG(I-1) + LNBR(I) = LNBR(I-1) + RNBR(I) = RNBR(I-1) + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) +10 CONTINUE + NCONTS = NCONTS - 1 + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + END + SUBROUTINE MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +GEL,FROM,TO,IDEVR,IDEVW,IDEVN,MAXGEL,KBOUT) +C Subroutine to move a gel from line from to line to +C Extended 22-5-91 +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),FROM,TO + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER NAMGEL*10,GEL(MAXGEL) + INTEGER GCLIN,CHAINL + LOGICAL LEFTE,RIGHTE + EXTERNAL GCLIN,CHAINL + LEFTE = .FALSE. + RIGHTE = .FALSE. +C +C left end ? +C + IF(LNBR(FROM).EQ.0) LEFTE = .TRUE. +C +C right end ? +C + IF(RNBR(FROM).EQ.0) RIGHTE = .TRUE. +C +C if both true remove the contig line, then overwrite the gel +C + IF(LEFTE.AND.RIGHTE) THEN + NCONTO = GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,FROM) + IF(NCONTO.EQ.0)THEN + WRITE(KBOUT,*) + + 'This gel has no left neighbour but does not' + WRITE(KBOUT,*)'appear in a contig line!' + ELSE + LNBR(NCONTO) = TO + RNBR(NCONTO) = TO + CALL WRITER(IDEVR,NCONTO,RELPG(NCONTO),LNGTHG(NCONTO), + + LNBR(NCONTO),RNBR(NCONTO)) + END IF + ELSE IF(LEFTE) THEN + NCONTO = GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,FROM) + IF(NCONTO.EQ.0)THEN + WRITE(KBOUT,*) + + 'This gel has no left neighbour but does not' + WRITE(KBOUT,*)'appear in a contig line!' + ELSE + LNBR(NCONTO) = TO + CALL WRITER(IDEVR,NCONTO,RELPG(NCONTO),LNGTHG(NCONTO), + + LNBR(NCONTO),RNBR(NCONTO)) + END IF + ELSE IF(RIGHTE) THEN + I = CHAINL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,FROM) + NCONTO = GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,I) + IF(NCONTO.EQ.0)THEN + WRITE(KBOUT,*) + + 'This gel has no right neighbour and does not' + WRITE(KBOUT,*)'appear in a contig!' + ELSE + IF(RNBR(NCONTO).NE.FROM)THEN + WRITE(KBOUT,*) + + 'This gel has no right neighbour but does not' + WRITE(KBOUT,*)'appear in a contig line!' + ELSE + RNBR(NCONTO) = TO + CALL WRITER(IDEVR,NCONTO,RELPG(NCONTO),LNGTHG(NCONTO), + + LNBR(NCONTO),RNBR(NCONTO)) + END IF + END IF + END IF + RELPG(TO)=RELPG(FROM) + LNGTHG(TO)=LNGTHG(FROM) + LNBR(TO)=LNBR(FROM) + RNBR(TO)=RNBR(FROM) + CALL READW(IDEVW,FROM,GEL,MAXGEL) + CALL WRITEW(IDEVW,TO,GEL,MAXGEL) + CALL READN(IDEVN,FROM,NAMGEL) + CALL WRITEN(IDEVN,TO,NAMGEL) + CALL WRITER(IDEVR,TO,RELPG(TO),LNGTHG(TO), + +LNBR(TO),RNBR(TO)) +C Do neighbours + IF(LNBR(FROM).NE.0) THEN + I=LNBR(FROM) + RNBR(I)=TO + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + END IF + IF(RNBR(FROM).NE.0) THEN + I=RNBR(FROM) + LNBR(I)=TO + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + END IF + CALL MOVTAG(FROM,TO) + END + SUBROUTINE DBOPEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,NAMPRO,GEL, + +IDBSIS,IDBSIZ,IERR,KBIN,KBOUT, + +IDEVR,IDEVW,IDEVN,IDEVT,IDEVC, + +MAXGEL,MAXGLM,LLINO, + +IDM,IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER GEL(MAXGLM) + INTEGER RELPG(IDBSIS) + INTEGER LNGTHG(IDBSIS),LNBR(IDBSIS),RNBR(IDBSIS) + CHARACTER NAMPRO*(*),COPYNO*4,HELPF*(*) + INTEGER IWORD,ANS + PARAMETER (IWORD=4) +C NOTE THIS IS THE MACHINES WORD LENGTH IE HOW MANY CHARS PER WORD + CALL FILLI(RELPG,IDBSIS,0) + CALL FILLI(LNGTHG,IDBSIS,0) + CALL FILLI(LNBR,IDBSIS,0) + CALL FILLI(RNBR,IDBSIS,0) + NAMPRO(1:)=' ' + IERR=1 +1 CONTINUE + L = 0 + CALL GTSTR('Project name',' ',NAMPRO,L,KBOUT,KBIN,INFLAG) + IF(L.LT.1) RETURN + LL = L + CALL CCASE(NAMPRO,1) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + L = 1 + CALL GTSTR('Version','0',COPYNO,L,KBOUT,KBIN,INFLAG) + CALL CCASE(COPYNO,1) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(L.LT.1) COPYNO(1:1) = '0' +C GET .ARN FIRST AS IT CONTAINS LENGTH OF OTHER FILES + NAMPRO(LL+1:LL+3)='.AR' + NAMPRO(LL+4:LL+4)=COPYNO(1:1) + CALL OPENRS(IDEVN,NAMPRO,IOK,3,4) + IF(IOK.NE.0)GO TO 100 +C ******* PROBLEM HERE READING RECORD 1000 AND INTEGER FROM IDEVN!!! + READ(IDEVN,REC=IDBSIS,ERR=50)IDBSIZ,MAXGEL,IDM + IF(MAXGEL.LT.512)GO TO 50 + IF(MAXGEL.GT.MAXGLM)GO TO 50 + IF(MOD(MAXGEL,512).NE.0)GO TO 50 +20 CONTINUE + IF((IDM.NE.5).AND.(IDM.NE.26))GO TO 51 +21 CONTINUE + NAMPRO(LL+2:LL+3)='RL' + CALL OPENRS(IDEVR,NAMPRO,IOK,4,4) + IF(IOK.NE.0)GO TO 100 + NAMPRO(LL+2:LL+3)='SQ' +C DEFINE RECORD LENGTH IN TERMS OF NUMBER OF CHARS PER WORD (4 ON VAX) +C AND MAXGEL SIZE + IREC=MAXGEL/IWORD + IF(MOD(MAXGEL,IWORD).NE.0)IREC=IREC+1 + CALL OPENRS(IDEVW,NAMPRO,IOK,IREC,4) + IF(IOK.NE.0)GO TO 100 +C NAMPRO(LL+2:LL+3) = 'RD' +C CALL OPENRS(IDEVRD,NAMPRO,IOK,7,4) +C IF(IOK.NE.0) IDEVRD = -9 + NAMPRO(LL+2:LL+3) = 'TG' + CALL OPENRS(IDEVT,NAMPRO,IOK,5,4) + IF(IOK.NE.0) IDEVT = -1 + NAMPRO(LL+2:LL+3) = 'CC' +C COMMENT_LENGTH: 11 = (40 + long)/long + CALL OPENRS(IDEVC,NAMPRO,IOK,11,4) + IF(IOK.NE.0) IDEVC = -1 +C READ A LINE FOR LUCK + CALL READW(IDEVW,1,GEL,MAXGEL) + CALL READR(IDEVR,IDBSIZ,NGELS,NCONTS,IDUM1,IDUM2) + WRITE(KBOUT,10011)NGELS,NCONTS,IDBSIZ,MAXGEL +10011 FORMAT(' Number of gel readings=',I3,' Number of contigs=',I3,/, + +' Database size=',I4,' Maximum gel reading length=',I4) + LLINO = 0 + IF(NGELS.LT.1)GO TO 5 + DO 3 I=1,NGELS + CALL READR(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) +3 CONTINUE + N=IDBSIZ-NCONTS + MXT = 0 + DO 4 I=N,IDBSIZ-1 + CALL READR(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + IF(RELPG(I).GT.MXT) THEN + MXT = RELPG(I) + LLINO = LNBR(I) + END IF +4 CONTINUE +5 CONTINUE + IERR=0 + NAMPRO(LL+2:LL+2) = COPYNO(1:1) + RETURN +100 CONTINUE + WRITE(KBOUT,9999) +9999 FORMAT(' Error encountered opening database files') + NAMPRO(LL+2:LL+2) = COPYNO(1:1) + RETURN +50 CONTINUE + WRITE(KBOUT,2000)MAXGLM +2000 FORMAT(' Error reading maximum gel reading length',/, + +' The maximum gel reading length must be a multiple', + +' of 512.',/,' E.G. 512, 1024, 1536, 2048, etc up to',I6) + MN = 512 + MX = MAXGLM + MAXIN1 = MN + CALL GETINT(MN,MX,MAXIN1, + +'Maximum gel reading length', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXGEL = IVAL + IF(MOD(MAXGEL,MAXIN1).NE.0)GO TO 50 +C PROBLEM WRITING TO RECORD IDBSIZ WITH NO SUBROUTINE CALL!!!!!!!!!! + WRITE(IDEVN,REC=IDBSIS)IDBSIZ,MAXGEL + GO TO 20 +51 CONTINUE + WRITE(KBOUT,2003) +2003 FORMAT(' Error reading protein or dna label') + CALL YESNO(ANS,'DNA', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) RETURN + IF(ANS.EQ.0)IDM = 5 + IF(ANS.EQ.1)IDM = 26 +C PROBLEM WRITING TO RECORD IDBSIZ WITH NO SUBROUTINE CALL!!!!!!!!!! + WRITE(IDEVN,REC=IDBSIS)IDBSIZ,MAXGEL,IDM + GO TO 21 + END + SUBROUTINE DBSTAR(NAMPRO,GEL,IDBSIS,IDBSIZ,KBIN,KBOUT, + +IDEV1,IDEV2,IDEV3,IDEVT,IDEVC, + +IERR,IHELPS,IHELPE,IDEVH,FILEH, + +MAXGEL,MAXGLM,IDM) + CHARACTER GEL(MAXGLM),FILEH*(*) + CHARACTER NAMPRO*(*) + INTEGER IWORD + PARAMETER (IWORD=4) + IERR=1 +3 CONTINUE + MN = 0 + CALL GTSTR('New project name',' ',NAMPRO,MN,KBOUT,KBIN,INFLAG) + IF(MN.LT.1) RETURN + LL = MIN(12,MN) + CALL CCASE(NAMPRO,1) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 3 + END IF + MN = 10 + MX = IDBSIS + IDBSIZ = 50 + CALL GETINT(MN,MX,IDBSIZ, + +'Database size', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IDBSIZ = IVAL +5 CONTINUE + MN = 512 + MX = MAXGLM + MAXIN1 = MIN(512,MAXGEL) + CALL GETINT(MN,MX,MAXIN1, + +'Maximum gel reading length', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXGEL = IVAL + IF(MOD(IVAL,MAXIN1).NE.0) THEN + MAXGEL = 512 + (IVAL/512)*512 + WRITE(KBOUT,*)'Maximum set to',MAXGEL + END IF + CALL YESNO(IDM,'Database is for DNA', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(IDM.LT.0) RETURN + IF(IDM.EQ.0)IDM = 5 + IF(IDM.EQ.1)IDM = 26 + NAMPRO(LL+1:LL+4)='.RL0' + CALL OPENRS(IDEV1,NAMPRO,IOK,4,3) + IF(IOK.NE.0)GO TO 100 +C IRAW = 0 +C IDEVRT = IDEVRD +C IDEVRD = -9 +C CALL YESNO(IRAW,'Create raw data pointer file', +C +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) +C IF(IRAW.LT.0) RETURN +C IF(IRAW.EQ.0) THEN +C NAMPRO(LL+1:LL+4)='.RD0' +C CALL OPENRS(IDEVRT,NAMPRO,IOK,7,3) +C IF(IOK.EQ.0) IDEVRD = IDEVRT +C END IF + WRITE(KBOUT,1004) +1004 FORMAT(' Writing new database files') + J = 0 + K = 0 + N = 0 + M = 0 + DO 10 I=1,IDBSIZ + CALL WRITER(IDEV1,I,J,K,M,N) +10 CONTINUE + NAMPRO(LL+2:LL+3)='SQ' + IREC=MAXGEL/IWORD + IF(MOD(MAXGEL,IWORD).NE.0)IREC=IREC+1 + CALL OPENRS(IDEV2,NAMPRO,IOK,IREC,3) + IF(IOK.NE.0)GO TO 100 + CALL FILLC(GEL,MAXGEL,' ') + CALL WRITEW(IDEV2,IDBSIZ,GEL,MAXGEL) + NAMPRO(LL+2:LL+3)='AR' + CALL OPENRS(IDEV3,NAMPRO,IOK,3,3) + IF(IOK.NE.0)GO TO 100 +C NOTE HERE IS A WRITE TO NAMES FILE NOT USING A SUBROUTINE CALL + WRITE(IDEV3,REC=IDBSIS)IDBSIZ,MAXGEL,IDM +C IF(IDEVRD.GT.0) THEN +C NAMFIL = ' ' +C MTYPE = ' ' +C I = 0 +C J = 0 +C K = 0 +C CALL WRITRD(IDEVRD,IDBSIZ,I,J,K,MTYPE,NAMFIL) +C ENDIF +C CREATE TAG FILES (TAGS AND COMMENTS) + IF (.TRUE.) THEN + NAMPRO(LL+2:LL+3)='TG' + CALL OPENRS(IDEVT,NAMPRO,IOK,5,3) + IF(IOK.NE.0)GO TO 100 + CALL WRITTG(IDEVT,IDBSIZ,IDBSIZ,0,0,0,0) + NAMPRO(LL+2:LL+3)='CC' +C COMMENT_LENGTH: 11 = (40 + long)/long + CALL OPENRS(IDEVC,NAMPRO,IOK,11,3) + IF(IOK.NE.0)GO TO 100 + CALL WRITCC(IDEVC,1,1,0,' ') + ENDIF + WRITE(KBOUT,1003)NAMPRO(1:LL),IDBSIZ +1003 FORMAT(' Database ',A,' version 0, size',I5, + +' successfully started') +C WRITE(KBOUT,1006)NAMPRO(1:LL) +1006 FORMAT( + +' Note the 3 database files are named ',A,'.AR0, .SQ0, .RL0') + IERR=0 + NAMPRO(LL+2:LL+2) = '0' + RETURN +100 CONTINUE + WRITE(KBOUT,9999) +9999 FORMAT(' Error when trying to open database,', + +' database not started') + RETURN + END + SUBROUTINE ENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,GEL2,IDBSIZ,IDEV,KBIN,KBOUT,IDEVR,IDEVW,IDEVN,IDEVG, + +IDEVT,IDEVC,LINLEN,PERCD,NAMARC, + +HELPS1,HELPE1,HELPS2,HELPE2,FILEH,IDEVH,MAXGEL,IDM,NCONTC) + CHARACTER FILEH*(*) + INTEGER HELPS1,HELPS2,HELPE1,HELPE2 +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER X,Y,ANS + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),NAMARC*(*) + CHARACTER NAMARK*10 + CHARACTER GEL2(MAXGEL) +C WRITE(KBOUT,1000) +C1000 FORMAT( +C +' Enter a new gel reading into the database.',//, +C +' Note that before a gel reading is entered into the',/, +C +' database it must be compared with the database to ',/, +C +' search for overlaps using a function such as auto assemble',/, +C +' The replies to all the questions that follow are given',/, +C +' by these searches',//) + IF((IDBSIZ-(NGELS+NCONTS)).GT.2)GO TO 5 + WRITE(KBOUT,1999)IDBSIZ +1999 FORMAT(' Current database size=',I5,' extend with copy') + RETURN +5 CONTINUE +C WRITE(KBOUT,1018) +C1018 FORMAT( +C +' We need the name of the file containing the gel reading') + CALL OPENF1(IDEVG,NAMARC,0,IOK,KBIN,KBOUT, + +'File name of gel reading to enter', + +HELPS1,HELPE1,FILEH,IDEVH) + IF(IOK.NE.0)RETURN + IF(NGELS.GT.0) THEN + DO 10 J=1,NGELS + CALL READN(IDEVN,J,NAMARK) + IF(NAMARC(1:10).NE.NAMARK)GO TO 10 + WRITE(KBOUT,1013)J +1013 FORMAT( ' Gel reading already in database (number,',I6, + + '), entry aborted') + RETURN +10 CONTINUE + END IF + IDIM=MAXGEL + CALL ARRFIM(IDEVG,GEL,IDIM,KBOUT) + CLOSE(UNIT=IDEVG) + NGELS=NGELS+1 + LNGTHG(NGELS)=IDIM + NAMARK=NAMARC(1:10) + CALL WRITEN(IDEVN,NGELS,NAMARK) + WRITE(KBOUT,1003)NGELS +1003 FORMAT( ' This gel reading has been given the number ',I6) + IF(IDEVT.GT.0) CALL ENTRD(IDEVG,IDEVRD,NAMARC,NGELS,IOK) + ANS=0 + IF(NGELS.GT.1)THEN +C WRITE(KBOUT,1017) +C1017 FORMAT( +C +' We need to know if the gel reading overlaps data already in', +C +' the database') + CALL YESNO(ANS,'New gel overlaps a contig', + + HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) THEN + NGELS = NGELS - 1 + RETURN + END IF + IF(ANS.EQ.0)GO TO 100 + END IF +C +C DOES NOT OVERLAP SO IT STARTS A CONTIG OF ITS OWN + LNBR(NGELS)=0 + RNBR(NGELS)=0 + RELPG(NGELS)=1 + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) + NCONTS=NCONTS+1 + N=IDBSIZ-NCONTS + LNBR(N)=NGELS + RNBR(N)=NGELS + RELPG(N)=(IDIM) + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + CALL WRITEW(IDEVW,NGELS,GEL,MAXGEL) + RETURN +C +100 CONTINUE +C WRITE(KBOUT,1019) +C1019 FORMAT( +C +' We need to know if the new gel reading overlaps in the',/, +C +' normal or complementary sense') +C COMPLEMENT GEL IF REQUIRED + CALL YESNO(ANS,'Gel overlaps in normal sense', + +HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) THEN + NGELS = NGELS - 1 + RETURN + END IF + IF(ANS.EQ.1) THEN + CALL SQREV(GEL,IDIM) + CALL SQCOM(GEL,IDIM) + LNGTHG(NGELS)=-1*LNGTHG(NGELS) + END IF +C WRITE(KBOUT,1020) +C1020 FORMAT( +C +' We need to know which contig the new gel reading overlaps') + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON,NCONTC, + +IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +HELPS1,HELPE1,FILEH,IDEVH) + IF(IERR.NE.0) THEN + NGELS=NGELS-1 + RETURN + END IF +C WRITE(KBOUT,1014) +C1014 FORMAT( +C +' We need information about the position of the overlap.',/, +C +' First there are two types: those that extend the contig',/, +C +' leftwards and those that start internally.') + CALL YESNO(ANS,'Left end of gel reading is inside contig', + + HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0)THEN + NGELS=NGELS-1 + RETURN + END IF + IF(ANS.EQ.1)GO TO 400 +C RIGHT END OR INTERNAL OVERLAP +C +C WRITE(KBOUT,1015) +C1015 FORMAT(' Now we need to know the exact position of the overlap') +160 CONTINUE + MN = 1 + MX = RELPG(LINCON) + X = 0 + CALL GETINT(MN,MX,X, + +'Position in contig of left end of gel reading', + +IVAL,KBIN,KBOUT, + +HELPS1,HELPE1,FILEH,IDEVH,IOK) + IF(IOK.NE.0) THEN + NGELS = NGELS - 1 + RETURN + END IF + X = IVAL + IF(X.EQ.0)GO TO 160 + N=NCONTC +200 CONTINUE + IF(RELPG(N).GT.X)GO TO 250 + IF(RNBR(N).EQ.0)GO TO 350 + N=RNBR(N) + GO TO 200 +250 CONTINUE +C GEL LIES BETWEEN N AND LNBR(N) +C DISPLAY JOINT + NOPT = 1 +C @jkb@ +C CALL BEDIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, +C +GEL,GEL2,LINCON,NCONTC,NOPT,X,IDBSIZ,IDEV,KBIN,KBOUT, +C +IDEVR,IDEVW,IDEVN,LINLEN,PERCD, +C +HELPS1,HELPE1,HELPS2,HELPE2,FILEH,IDEVH,MAXGEL,IDM) +C CHECK ON RESULT + IF(NOPT.EQ.1)GO TO 160 + IF(NOPT.EQ.3)GO TO 260 +C COCKUP + NGELS=NGELS-1 + RETURN +260 CONTINUE + CALL WRITEW(IDEVW,NGELS,GEL,MAXGEL) + LNBR(NGELS)=LNBR(N) + RNBR(NGELS)=N + RELPG(NGELS)=X + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) + RNBR(LNBR(N))=NGELS + K=LNBR(N) + CALL WRITER(IDEVR,K,RELPG(K),LNGTHG(K), + +LNBR(K),RNBR(K)) + LNBR(N)=NGELS + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + IDIM=ABS(LNGTHG(NGELS)) + Y=X+(IDIM)-1 + IF(Y.LE.RELPG(LINCON))RETURN + RELPG(LINCON)=Y + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) + RETURN +350 CONTINUE +C MUST BE A RIGHT END OVERLAP +C DSPLAY JOINT + NOPT = 1 +C @jkb@ +C CALL BEDIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, +C +GEL,GEL2,LINCON,NCONTC,NOPT,X,IDBSIZ,IDEV,KBIN,KBOUT, +C +IDEVR,IDEVW,IDEVN,LINLEN,PERCD, +C +HELPS1,HELPE1,HELPS2,HELPE2,FILEH,IDEVH,MAXGEL,IDM) + IF(NOPT.EQ.1)GO TO 160 + IF(NOPT.EQ.3)GO TO 360 +C COCKUP + NGELS=NGELS-1 + RETURN +360 CONTINUE + CALL WRITEW(IDEVW,NGELS,GEL,MAXGEL) + LNBR(NGELS)=N + RNBR(NGELS)=0 + RELPG(NGELS)=X + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) + RNBR(N)=NGELS + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) + RNBR(LINCON)=NGELS + IDIM=ABS(LNGTHG(NGELS)) + Y=X+(IDIM)-1 + RELPG(LINCON)=MAX(RELPG(LINCON),Y) + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + RETURN +C +400 CONTINUE +C +C ADDING TO LEFT END +C WRITE(KBOUT,1015) +410 CONTINUE + MN = 1 + MX = IDIM + X = 0 + CALL GETINT(MN,MX,X, + +'Position in new gel reading of left end of contig', + +IVAL,KBIN,KBOUT, + +HELPS1,HELPE1,FILEH,IDEVH,IOK) + IF(IOK.NE.0) THEN + NGELS = NGELS - 1 + RETURN + END IF + X = IVAL + IF(X.EQ.0)GO TO 410 +C SET FLAG TO SHOW LEFT END OVERLAP + NOPT=2 +C @jkb@ +C CALL BEDIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, +C +GEL,GEL2,LINCON,NCONTC,NOPT,X,IDBSIZ,IDEV,KBIN,KBOUT, +C +IDEVR,IDEVW,IDEVN,LINLEN,PERCD, +C +HELPS1,HELPE1,HELPS2,HELPE2,FILEH,IDEVH,MAXGEL,IDM) + IF(NOPT.EQ.1)GO TO 400 + IF(NOPT.EQ.3)GO TO 420 +C COCKUP + NGELS=NGELS-1 + RETURN +420 CONTINUE + CALL WRITEW(IDEVW,NGELS,GEL,MAXGEL) + RELPG(NGELS)=1 + RNBR(NGELS)=NCONTC + LNBR(NGELS)=0 + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) + LNBR(NCONTC)=NGELS + RELPG(NCONTC)=X + CALL WRITER(IDEVR,NCONTC,RELPG(NCONTC),LNGTHG(NCONTC), + +LNBR(NCONTC),RNBR(NCONTC)) + RELPG(LINCON)=RELPG(LINCON)+X-1 + IDIM=ABS(LNGTHG(NGELS)) + Y=IDIM + IF(Y.GT.RELPG(LINCON))RELPG(LINCON)=Y + LNBR(LINCON)=NGELS + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + N=NCONTC +440 CONTINUE + IF(RNBR(N).EQ.0)RETURN + N=RNBR(N) + RELPG(N)=RELPG(N)+X-1 + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) + GO TO 440 + END +C SUBROUTINE XENTRD(IDEVG,IDEVRD,NAMARC,NGEL,IOK) +C CHARACTER NAMARC*(*),NAMFIL*12,MTYPE*4 +CC Enters raw data info into database +C IOK = 0 +C IF(IDEVRD.GT.0) THEN +C CALL OPENRS(IDEVG,NAMARC,IOK,LRECL,2) +CC IF(IOK.EQ.0) THEN +C CALL RRD(IDEVG,LENR,LCUT,LENW,MTYPE,NAMFIL) +C CALL WRITRD(IDEVRD,NGEL,LENR,LCUT,LENW,MTYPE,NAMFIL) +CC END IF +C END IF +C END + SUBROUTINE FIXRD(IDEVT,IDEVC,IDBSIZ,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH) +C FILE_NAME_LENGTH + CHARACTER NAMFIL*18,NEWNAM*18,MTYPE*4,NEWMT*4,FILEH*(*) + IF(IDEVRD.LT.0) THEN + WRITE(KBOUT,*)'No raw data file!' + RETURN + END IF + 10 CONTINUE +C Change raw data record + MN = 0 + MX = IDBSIZ-1 + LNO = 0 + CALL GETINT(MN,MX,LNO, + + 'Number of line to change', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF(IVAL.EQ.0) RETURN + LNO = IVAL + CALL READRD(IDEVT,IDEVC,LNO,LENR,LCUT,LENW,MTYPE,NAMFIL) + WRITE(KBOUT,*)'Current line' + WRITE(KBOUT,1001)LENR,LCUT,LENW,MTYPE,NAMFIL +1001 FORMAT(' ',3I6,' ',A,' ',A) + MN = 1 + MX = 9999 + LX = LENR + CALL GETINT(MN,MX,LX, + + 'Length raw sequence', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + LX = IVAL + MN = 1 + MX = LX + L = LCUT + CALL GETINT(MN,MX,L, + + 'Left cutoff', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + L = IVAL + MN = 1 + MX = LX + M = LENW + CALL GETINT(MN,MX,M, + + 'Length of original working sequence', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + M = IVAL + 20 CONTINUE + LNAM = 4 + CALL GTSTR('Machine type', + + MTYPE,NEWMT,LNAM,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 20 + END IF + IF(INFLAG.EQ.3) NEWMT = MTYPE + 30 CONTINUE +C FILE_NAME_LENGTH + LNAM = 18 + CALL GTSTR('Name for raw data file', + + NAMFIL,NEWNAM,LNAM,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 30 + END IF + IF(INFLAG.EQ.3) NEWNAM = NAMFIL + WRITE(KBOUT,1001)LX,L,M,NEWMT,NEWNAM + CALL WRITRD(IDEVT,IDEVC,LNO,LX,L,M,NEWMT,NEWNAM) + WRITE(KBOUT,*)'New record written to disk' + GO TO 10 + END +C SUBROUTINE READRD(IDEVT,IDEVC,NGEL,LENR,LCUT,LENW,MTYPE,NAMFIL) +C CHARACTER MTYPE*(*),NAMFIL*(*) +C IF(IDEVT.GT.0)READ(IDEVRD,REC=NGEL)LENR,LCUT,LENW,MTYPE,NAMFIL +C END +C SUBROUTINE RRD(IDEVG,LENR,LCUT,LENW,MTYPE,NAMFIL) +C CHARACTER LINE*80,NAMFIL*(*),MTYPE*(*) +C Reads raw data info from reading file assuming it starts with ; +C 1 CONTINUE +C READ(IDEVG,1000,END=100,ERR=100)LINE +C 1000 FORMAT(A) +C IF(LINE(1:1).NE.';') GO TO 1 +C READ(LINE,1001,ERR=100)LENR,LCUT,LENW,MTYPE,NAMFIL +C 1001 FORMAT(1X,3I6,A,A) +C RETURN +C 100 CONTINUE +C LENR = 0 +C LCUT = 0 +C LENW = 0 +C MTYPE = ' ' +C NAMFIL = ' ' +C END +C SUBROUTINE WRITRD(IDEVT,IDEVC,NGEL,LENR,LCUT,LENW,MTYPE,NAMFIL) +C CHARACTER MTYPE*(*),NAMFIL*(*) +C IF(IDEVT.GT.0)WRITE(IDEVRD,REC=NGEL)LENR,LCUT,LENW,MTYPE,NAMFIL +C END + SUBROUTINE READRD(IDEVT,IDEVC,NGEL,LENR,LCUT,LENW,MTYPE,NAMFIL) + CHARACTER MTYPE*(*),NAMFIL*(*) +C COMMENT_LENGTH + CHARACTER NOTE*40 + IF(IDEVT.GT.0)THEN + CALL READTG(IDEVT,NGEL,LPOS,LLEN,LCOM,LTYPE,NEXT) + CALL READCC(IDEVC,LCOM,ICNT,NEXT,NOTE) + READ(NOTE,1001,ERR=100)LENR,LCUT,LENW,MTYPE,NAMFIL + 1001 FORMAT(3I6,A,A) + ENDIF + RETURN + 100 CONTINUE + LENR = 0 + LCUT = 0 + LENW = 0 + MTYPE = ' ' + NAMFIL = ' ' + END + SUBROUTINE WRITRD(IDEVT,IDEVC,NGEL,LENR,LCUT,LENW,MTYPE,NAMFIL) + CHARACTER MTYPE*(*),NAMFIL*(*) + INTEGER FREECC +C COMMENT_LENGTH + CHARACTER NOTE*40 + IF(IDEVT.GT.0)THEN + CALL READTG(IDEVT,NGEL,LPOS,LLEN,LCOM,LTYPE,NEXT) + IF(LCOM.EQ.0)THEN + LCOM = FREECC(IDEVC) + ENDIF + WRITE(NOTE,1001,ERR=100)LENR,LCUT,LENW,MTYPE,NAMFIL + 1001 FORMAT(3I6,A,A) + NEXT = 0 + CALL WRITCC(IDEVC,LCOM,ICNT,NEXT,NOTE) + ENDIF + RETURN + 100 CONTINUE + END + SUBROUTINE READTG(IDEVT,I,LPOS,LLEN,LCOM,LTYPE,NEXT) + IF(IDEVT.GT.0) THEN + READ(IDEVT,REC=I)LPOS,LLEN,LCOM,LTYPE,NEXT + ENDIF + END + SUBROUTINE WRITTG(IDEVT,I,LPOS,LLEN,LCOM,LTYPE,NEXT) + IF (IDEVT.GT.0) THEN + WRITE(IDEVT,REC=I)LPOS,LLEN,LCOM,LTYPE,NEXT + ENDIF + END + SUBROUTINE READCC(IDEVC,I,ICNT,NEXT,NOTE) +C COMMENT_LENGTH + CHARACTER NOTE*40 +C COMMENT_LENGTH - 4 + CHARACTER DUMM*36 + IF(IDEVC.GT.0)THEN + READ(IDEVC,REC=1)NEXT,ICNT,DUMM + IF(I.EQ.0.OR.I.GT.ICNT)THEN + NEXT = 0 + NOTE = ' ' + ELSE + READ(IDEVC,REC=I)NEXT,NOTE + ENDIF + ENDIF + END + SUBROUTINE WRITCC(IDEVC,I,ICNT,NEXT,NOTE) +C COMMENT_LENGTH + CHARACTER NOTE*40 +C COMMENT_LENGTH - 4 + CHARACTER DUMM*36 + IF(IDEVC.GT.0)THEN + IF(I.EQ.1) THEN + WRITE(IDEVC,REC=1)NEXT,ICNT,DUMM + ELSE + READ(IDEVC,REC=1)IDUM,ICNT,DUMM + IF(I.LE.ICNT) WRITE(IDEVC,REC=I)NEXT,NOTE + ENDIF + ENDIF + END + SUBROUTINE PADCON(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,LINCON,POSN,NC,IDBSIZ,IDEVR,IDEVW,MAXGEL,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),POSN,X + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + CHARACTER PAD + SAVE PAD + DATA PAD/'*'/ +C NOW FIND FIRST CHAR THAT OVERLAPS REGION + LLINO=LNBR(LINCON) +30 CONTINUE + X=RELPG(LLINO)+ABS(LNGTHG(LLINO))-1 + IF(X.GE.POSN)GO TO 40 +C NOT IN REGION + LLINO=RNBR(LLINO) + GO TO 30 +40 CONTINUE +C NOW GET THIS GEL FROM DISK +C READ(IDEVW,REC=LLINO)GEL + CALL READW(IDEVW,LLINO,GEL,MAXGEL) +C CALC POSN IN THIS GEL TO EDIT + X=POSN-RELPG(LLINO)+1 + K=X +C MOVE THE DATA RIGHT + M=ABS(LNGTHG(LLINO)) + LNGTHG(LLINO)=LNGTHG(LLINO)+SIGN(NC,LNGTHG(LLINO)) +C CHECK FOR OVER END OF ARRAY + N=ABS(LNGTHG(LLINO)) + IF(N.GT.MAXGEL)THEN + WRITE(KBOUT,1000)LLINO +1000 FORMAT( + +' Data pushed off end of gel',I4,' during padding') + M=M-(N-MAXGEL) + N=MAXGEL + LNGTHG(LLINO)=SIGN(MAXGEL,LNGTHG(LLINO)) + END IF + J=M-K+1 + DO 55 I=1,J + GEL(N)=GEL(M) + N=N-1 + M=M-1 +55 CONTINUE +C PERFORM THE INSERTION + DO 60 I=K,MIN(MAXGEL,K+NC-1) + GEL(I)=PAD +60 CONTINUE +C WRITE BACK TO DISK + CALL WRITEW(IDEVW,LLINO,GEL,MAXGEL) +C WRITE(IDEVW,REC=LLINO)GEL +C WRITE NEW LINE + CALL WRITER(IDEVR,LLINO,RELPG(LLINO),LNGTHG(LLINO), + +LNBR(LLINO),RNBR(LLINO)) +C WRITE(IDEVR,REC=LLINO) +C 1RELPG(LLINO),LNGTHG(LLINO),LNBR(LLINO),RNBR(LLINO) +C +C NOW UPDATE TAG FILES ACCORDINGLY + CALL PADTAG(LLINO,K,NC,LNGTHG(LLINO)) +C +65 CONTINUE +C NOW GET NEXT GEL + LLINO=RNBR(LLINO) +C LAST GEL? + IF(LLINO.EQ.0)GO TO 70 +C DOES IT HAVE DATA IN REGION? +C IE DO RELPG AND RELPG+LNGTHG-1 LIE EITHER SIDE OF POSN? + IF(RELPG(LLINO).GT.POSN)GO TO 70 + X=RELPG(LLINO)+ABS(LNGTHG(LLINO))-1 + IF(X.LT.POSN)GO TO 65 +C WITHIN + GO TO 40 +70 CONTINUE +C INSERTS FINISHED SO NEED TO INCREMENT ALL THOSE GELS TO RIGHT + LLINO=LNBR(LINCON) +75 CONTINUE + IF(RELPG(LLINO).GT.POSN)GO TO 80 +76 CONTINUE + LLINO=RNBR(LLINO) + IF(LLINO.EQ.0)GO TO 90 + GO TO 75 +80 CONTINUE + RELPG(LLINO)=RELPG(LLINO)+NC +C WRITE NEW LINE + CALL WRITER(IDEVR,LLINO,RELPG(LLINO),LNGTHG(LLINO), + +LNBR(LLINO),RNBR(LLINO)) +C WRITE(IDEVR,REC=LLINO) +C 1RELPG(LLINO),LNGTHG(LLINO),LNBR(LLINO),RNBR(LLINO) + GO TO 76 +90 CONTINUE +C NEED TO INCREMENT CONTIG LINE + RELPG(LINCON)=RELPG(LINCON)+NC + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) +C WRITE(IDEVR,REC=LINCON)RELPG(LINCON),LNGTHG(LINCON),LNBR(LINCON), +C 1RNBR(LINCON) + RETURN + END + SUBROUTINE AUTOJ(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ, + +NGELS,NCONTS,MAXGEL, + +TEMP3,WORDP,WORDN,LPOWRC,POSNS,GELN, + +SEQ1,MAXSEQ,SEQ2,SEQ3,SEQ4,SEQ5,SEQC2,SEQG2,MATCH, + +MAXGLM,MAXGL2,CHRSIZ,ECHRSZ,LENGTH, + +SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON,CONST, + +KBIN,KBOUT,IDEV1,IDEV2,IDEV3,IDEV4,IDEV7,IDEV8,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,NAMARC,NAMPRO,FILE, + +PERCD,IOPEN,IDM,SEQG3,SEQC3,IOK) + INTEGER CHRSIZ,ECHRSZ + INTEGER RELPG(MAXDB) + INTEGER LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + INTEGER JOINT(2),ITOTPC(2),ITOTPG(2),IDIM22(2),IDOUT(2) + INTEGER LLINO(2),ITYPE(2),IFAIL(2) + INTEGER ILEFTS(2),ILC(2),IPOSC(2),IPOSG(2),ISENSE(2) + INTEGER ANS,WINDOW + INTEGER TEMP3(ECHRSZ,MAXGL2),CONST(LENGTH) + INTEGER POSNS(MAXSEQ),WORDP(LPOWRC),WORDN(LPOWRC),GELN(MAXGLM) + INTEGER CENDS(MAXCON),NENDS(MAXCON) + CHARACTER SEQ3(MAXGLM),SEQC2(MAXGLM,2),SEQG2(MAXGLM,2) + CHARACTER SEQ1(MAXSEQ),SEQ2(MAXGLM),MATCH(MAXGLM),SEQ4(MAXGLM) + INTEGER SAV1(MAXSAV),SAV2(MAXSAV),SAV3(MAXSAV) + CHARACTER NAMARC*(*),NAMPRO*(*),FILE*(*) + CHARACTER SEQ5(MAXGLM),HELPF*(*),SEQG3(MAXGLM),SEQC3(MAXGLM) + CALL DBCHEK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + TEMP3,IERR,KBOUT) + IF(IERR.GT.1) RETURN + IFAIL(1) = 0 + IF(NGELS.LT.1) RETURN + MN = LENGTH*2 + MX = MAXGLM + 1 + MINMAT = MAX(15,MN) + CALL GETINT(MN,MX,MINMAT, + +'Minimum initial match', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINMAT = IVAL + MN = 2 + MX = 5 + MINSLI = MAX(3,MN) + CALL GETINT(MN,MX,MINSLI, + +'Minimum alignment block', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINSLI = IVAL + MN = 0 + MX = 50 + MAXPG = 8 + CALL GETINT(MN,MX,MAXPG, + +'Maximum pads per sequence', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXPG = IVAL + MAXPC = IVAL + RMN = 0. + RMX = 100. + PERMAX = 8. + CALL GETRL(RMN,RMX,PERMAX, + + 'Maximum percent mismatch after alignment', + + VAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + PERMAX = VAL + MN = MINMAT + MX = MAXGEL + WINDOW = 100 + CALL GETINT(MN,MX,WINDOW, + +'Probe length', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + WINDOW = IVAL + IOK = 1 + I = 0 + CALL YESNO(I,'Use clipped data', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(I.LT.0) RETURN + IWING = 0 + IF(I.EQ.0) THEN + MN = 1 + MX = MAXGEL + IWING = 100 + CALL GETINT(MN,MX,IWING, + + 'Window size for good data scan', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IWING = IVAL + MN = 1 + MX = MIN(100,IWING) +C Note nbad must be compatible with nok2 + NBAD = MIN(IWING,5) + CALL GETINT(MN,MX,NBAD, + + 'Maximum number of dashes in scan window', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + NBAD = IVAL + END IF + IDIM1=0 + MAXOVR=MAXGEL-3*MAX(MAXPC,MAXPG) + ANS=0 + CALL JCONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,MAXSEQ,SEQ2,IDBSIZ,IDIM1,ANS,KDUMM,KDUMM,KDUMM,TEMP3, + +ECHRSZ,MAXGL2,KBOUT,IDEV2,IFAIL(1),MAXGEL,IDM,PERCD,SEQ5, + +CENDS,NENDS,MAXCON,IWING,NBAD) + IDIM2=MAXGEL +C +C Note I am doing something horrible here to save space: +C sending cends and nends to jcons to get the extension lengths +C then swapping them with temp3 by sending temp to ajoin and +C receiving them as cends and nends, and sending cends nends +C and receiving them as iladd,iradd + CALL AUTOJN(SEQ1,IDIM1,SEQ2,IDIM2,ILEFTS,ILC,IPOSC, + +IPOSG,ISENSE,LLINO,IMATC,IFCOMP,MINMAT,POSNS,WORDP,WORDN, + +CONST,LENGTH,LPOWRC,IDEV,MATCH,MAXGEL,MAXGLM,SEQ5,GELN, + +SAV1,SAV2,SAV3,MAXSAV,TEMP3,TEMP3(MAXCON+1,1),MAXCON, + +SEQG2,SEQC2,SEQ4,IDOUT,IDIM22,ITOTPG,ITOTPC,JOINT,IFAIL, + +ITYPE,MAXPC,MAXPG,PERMAX,MINSLI,SEQG3,SEQC3,KFAIL, + +WINDOW,CENDS,NENDS,RELPG,LNBR,IDBSIZ,NCONTS) + END + SUBROUTINE AUTOJN(SEQ1,IDIMIN,GEL,IDIMGI,ILEFTS,ILC,IPOSC, + +IPOSG,ISENSE,LLINO,IMATC,IFCOMP,MINMAT,POSNS,WORDP,WORDN, + +CONST,LENGTH,LPOWRC,KBOUT,MATCH,MAXGEL,MAXGLM,GELCOP,GELN, + +SAVPS,SAVPG,SAVL,MAXSAV,CENDS,NENDS,MAXCON, + +SEQG2,SEQC2,SEQ4,IDOUT,IDIM22,ITOTPG,ITOTPC,JOINT,IFAIL, + +ITYPE,MAXPC,MAXPG,PERMAX,MINSLI,SEQG3,SEQC3,KFAIL, + +WINDOW,ILADD,IRADD,RELPG,LNBR,IDBSIZ,NCONTS) +C AUTHOR: RODGER STADEN + INTEGER ILEFTS(2),ILC(2),IPOSC(2),IPOSG(2),ISENSE(2),LLINO(2) + INTEGER POSNS(IDIMIN),GELN(MAXGLM),WORDP(LPOWRC),SAVPS(MAXSAV) + INTEGER SAVPG(MAXSAV),SAVL(MAXSAV) + INTEGER WORDN(LPOWRC),RELPG(IDBSIZ),LNBR(IDBSIZ) + CHARACTER GELCOP(MAXGLM),MATCH(MAXGLM) + INTEGER CENDS(MAXCON),NENDS(MAXCON),ILADD(MAXCON),IRADD(MAXCON) + INTEGER CONST(LENGTH) + CHARACTER SEQ1(IDIMIN),GEL(MAXGLM) + CHARACTER SEQG2(MAXGLM,2),SEQC2(MAXGLM,2),SEQ4(MAXGLM) + INTEGER IDOUT(2),IDIM22(2),ITOTPG(2),ITOTPC(2),JOINT(2) + INTEGER IFAIL(2),ITYPE(2) + PARAMETER (MAXC = 10) + CHARACTER SEQG3(MAXGLM),SEQC3(MAXGLM) + INTEGER JLEFTS(MAXC),JLC(MAXC),JPOSC(MAXC),JPOSG(MAXC),MCON(MAXC) + INTEGER JSENSE(MAXC),JLLINO(MAXC),WINDOW + IFAIL(1) = 1 + IFAIL(2) = 1 + KFAIL = 0 + IDCEND=MAXCON + CALL FNDCON(SEQ1,IDIMIN,CENDS,NENDS,IDCEND,MAXCON,KBOUT) +C find possible missed joins +C we have consensus in seq1 in order first contig,second contig etc +C compare the ends in reverse order, simultaneously shortening the consensus + IDIM = IDIMIN + DO 200 JCON = IDCEND,2,-1 + IDIM = CENDS(JCON) - 1 + CALL ENCO(SEQ1,IDIM,POSNS,CONST,LENGTH) + CALL ENCONA(POSNS,IDIM,WORDP,WORDN,LPOWRC,LENGTH) + JS = CENDS(JCON) + 20 + JE = CENDS(JCON+1) - 1 + IEND = 1 + IDIMG = MIN(WINDOW,JE-JS+1) +C check for case where contig is shorter than probe (window) +C in which case only compare the left hand end + IF(JE-JS+1.LE.WINDOW) IEND = 2 +C WRITE(*,*)JCON,JS,JE,IDIMG +1 CONTINUE + IMATC = 0 + IF(IEND.EQ.1) THEN + CALL SQCOPY(SEQ1(JE-IDIMG+1),GEL,IDIMG) + ELSE + CALL SQCOPY(SEQ1(JS),GEL,IDIMG) + END IF + CALL SQCOPY(GEL,GELCOP,IDIMG) + ISTRAN=1 +2 CONTINUE + CALL ENCO(GEL,IDIMG,GELN,CONST,LENGTH) + IDSAV=MAXSAV + CALL CFGEL(GELN,IDIMG,POSNS,IDIM,WORDP,WORDN,LENGTH,LPOWRC, + +SAVPG,SAVPS,SAVL, + +IDSAV,SEQ1,GEL,MINMAT,IFCOMP,KBOUT) + IF(IFCOMP.NE.0) THEN + CALL ERROM(KBOUT,'Error in CFGEL') + RETURN + END IF + IF(IDSAV.NE.0)THEN + CALL ADISM5(IDIM,IDIMG,SAVPS,SAVPG,IDSAV,CENDS,NENDS, + + IDCEND,MAXCON,JLEFTS,JLC,JPOSC,JPOSG,JSENSE,JLLINO, + + IMATC,ISTRAN,MAXC,KBOUT,MCON) + END IF + ISTRAN=ISTRAN+1 + IF(ISTRAN.EQ.2) THEN + CALL SQCOPY(GELCOP,GEL,IDIMG) + CALL SQREV(GEL,IDIMG) + CALL SQCOM(GEL,IDIMG) + GO TO 2 + END IF + CALL SQCOPY(GELCOP,GEL,IDIMG) + KSENSE = 0 +C WRITE(KBOUT,*)'Total matches found',IMATC + IF(IMATC.NE.0) THEN + JMATC = 0 + DO 100 I = 1,IMATC + IF(JSENSE(I).EQ.-1) THEN + IF(KSENSE.EQ.0) THEN + CALL SQREV(GEL,IDIMG) + CALL SQCOM(GEL,IDIMG) + KSENSE = 1 + END IF + END IF + JDIM22 = IDIMG + JDOUT = MAXGEL + IDSAV = MAXSAV +C IF(IEND.EQ.1) WRITE(KBOUT,*)'Left end of Contig',NENDS(JCON) +C IF(IEND.EQ.2) WRITE(KBOUT,*)'Right end of Contig',NENDS(JCON) +C WRITE(KBOUT,*)'Trying to align with contig',JLLINO(I) + CALL ALINEJ(SEQ1(JLEFTS(I)),GEL,SEQG3,SEQC3, + + SAVPS,SAVPG,SAVL,IDSAV,JLC(I),JDIM22,JDOUT, + + JPOSC(I),JPOSG(I),MINSLI,JJOINT,JTOTPC,JTOTPG, + + JFAIL,JTYPE,MAXPC,MAXPG,PERMAX,KBOUT,SEQ4,MAXGEL,PERMS, + + NENDS(JCON),IEND,JLLINO(I),JSENSE(I),ILADD,IRADD,MAXCON,MCON(I), + + JCON,RELPG,LNBR,IDBSIZ,NCONTS,WINDOW) +100 CONTINUE + END IF + IEND = IEND + 1 + IF(IEND.EQ.2) GO TO 1 +C IDIM = CENDS(JCON)-1 + 200 CONTINUE + END + SUBROUTINE ALINEJ(SEQ1,SEQ2,SEQG2,SEQC2,ISAV1,ISAV2,ISAV3, + +IDSAV,IDC,IDIM2,IDOUT,IC1,IG1,MINSLI,JOINT, + +ITOTPC,ITOTPG,IFAIL,ITYPE,MAXPC,MAXPG,PERMAX,KBOUT,SEQ3,MAXGEL, + +PERCM,JCONN,IEND,NCON,JSENSE,ILADD,IRADD,MAXCON,MCON,JCON, + +RELPG,LNBR,IDBSIZ,NCONTS,WINDOW) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDC),SEQ2(IDIM2),SEQG2(IDOUT),SEQC2(IDOUT) + CHARACTER SEQ3(MAXGEL) + INTEGER ISAV1(IDSAV),ISAV2(IDSAV),ISAV3(IDSAV) + INTEGER ILADD(MAXCON),IRADD(MAXCON) + INTEGER RELPG(IDBSIZ),LNBR(IDBSIZ),WINDOW + MINSLT=MINSLI +C SAVE SEQ2 + CALL SQCOPY(SEQ2,SEQ3,IDIM2) + CALL MSTLKL(SEQ3,IDIM2) + IFAIL=1 +C FIND MATCHES + IPP=IDSAV + CALL SLIDER(SEQ1,IDC,SEQ3,IDIM2,IC1,IG1,MAXPG,MAXPC,MINSLT, + +ISAV1,ISAV2,ISAV3,IPP) + IF(IPP.GT.IDSAV)RETURN + IF(IPP.LT.1)RETURN + CALL REMOVL(ISAV2,ISAV3,ISAV1,IPP) + CALL BUB3AS(ISAV2,ISAV3,ISAV1,IPP) +C DO TOPOLOGICAL CHECK + CALL TPCHEK(ISAV2,ISAV3,ISAV1,IPP) + IFAIL=0 + CALL LINEUP(SEQ2,SEQ1,SEQG2,SEQC2,IDC,IDIM2,IDOUT,ISAV3,ISAV2, + +ISAV1,IPP,ITOTPC,ITOTPG,JOINT,ITYPE,KBOUT,MAXGEL,IFAIL) + IF(IFAIL.NE.0)RETURN +C IDIM2 IS NOW LENGTH OF ALIGNED GEL +C WRITE(KBOUT,1052)ITOTPC,ITOTPG +C1052 FORMAT(' Padding in contig= ',I4,' and in gel= ',I4) + CALL JALIGN(SEQC2,SEQG2,SEQ3,MAXGEL,IDOUT,IDIM2,JOINT, + +ITYPE,PERCM,KBOUT,IFAIL,PERMAX,JCONN,IEND,NCON,JSENSE, + +ILADD,IRADD,MAXCON,MCON,JCON,RELPG,LNBR,IDBSIZ,NCONTS,WINDOW) +C ARE ALL CHECKS OK? + IF(ITOTPC.GT.MAXPC)IFAIL=1 + IF(ITOTPG.GT.MAXPG)IFAIL=1 + IF(PERCM.GT.PERMAX)IFAIL=1 + END +C SUBROUTINE DALIGN +C +C COUNTS MISMATCHES AND DISPLAYS OVERLAP. + SUBROUTINE JALIGN(SEQC2,SEQG2,SEQ3,MAXGEL,IDOUT,IDIM2, + +JOINT,ITYPE,X,KBOUT,IFAIL,PERMAX,JCONN,IEND,NCON,JSENSE, + +ILADD,IRADD,MAXCON,MCON,JCON,RELPG,LNBR,IDBSIZ,NCONTS,WINDOW) +C AUTHOR: RODGER STADEN + CHARACTER SEQC2(MAXGEL),SEQG2(MAXGEL),SEQ3(MAXGEL) + CHARACTER PAD,DASH,STRAND,NAME1*6,NAME2*6 + INTEGER ILADD(MAXCON),IRADD(MAXCON),RELPG(IDBSIZ),LNBR(IDBSIZ) + INTEGER CLINNO,WINDOW + EXTERNAL CLINNO + SAVE PAD,DASH + DATA PAD,DASH/',','-'/ +C +C where are the overlaps? +C +C +C +C if ITYPE is 1 the overlap starts within the reading at JOINT +C else it starts at the left end of the reading at JOINT in the contig + IENDG=1 + IENDC=JOINT +C ONLY LOOK AT OVERLAP WHICH IS FROM JOINT FOR LEFT TYPE JOIN + IF(ITYPE.EQ.1)THEN + IENDG=JOINT + IENDC=1 + END IF +100 CONTINUE +C LENGTH OF OVERLAP? + LG=IDIM2-IENDG+1 + LO=MIN(IDOUT,LG) +C SAVE RAW DATA + CALL SQCOPY(SEQG2,SEQ3,IDIM2) + CALL MSTLKL(SEQ3,IDIM2) + X=FLOAT(LO) + Y=X + K=IENDG+LO-1 +C POINT TO CONSENSUS + J=0 +C CHECK FOR OVERFLOW + IF(K.GT.MAXGEL)THEN + WRITE(KBOUT,1001) +1001 FORMAT( + +' Matching region too long for routine dalign. Alignment aborted') + IFAIL=1 + RETURN + END IF + DO 200 I=IENDG,K + J=J+1 + IF(SEQC2(J).EQ.SEQ3(I))GO TO 200 +C IF(SEQ3(I).EQ.DASH)GO TO 200 +C IF(SEQC2(J).EQ.DASH)GO TO 200 +C IF(SEQC2(J).EQ.PAD)GO TO 200 + X=X-1. +200 CONTINUE + X=(Y-X)*100./Y + IF(X.LT.PERMAX) THEN + IC = CLINNO(LNBR,IDBSIZ,NCONTS,JCONN) + IF(IC.EQ.0) THEN + IFAIL = 99 + RETURN + END IF + IF(JSENSE.EQ.-1) THEN + STRAND = '-' + IF(IEND.EQ.1) THEN +C +C probe is complement of right hand end of contig. Give posns assuming +C this contig is complemented. +C + IENDGT = IENDG - IRADD(JCON) +C +C Next line gives posns relative to original orientation +C IENDGT = RELPG(IC) + IRADD(JCON) - IENDG + 1 + ELSE +C +C probe is complement of left end of contig. Give posns assuming this +C contig is going to be complemented. +C + IENDGT = RELPG(IC) - WINDOW + ILADD(JCON) + IENDG + END IF + ELSE + STRAND = '+' + IF(IEND.EQ.2) THEN +C +C probe is left hand end of contig in original sense +C + IENDGT = IENDG - ILADD(JCON) + ELSE +C +C probe is right hand end of contig in original sense +C + IENDGT = RELPG(IC) + IRADD(JCON) - WINDOW + IENDG + END IF + END IF + IENDCT = IENDC - ILADD(MCON) + WRITE(KBOUT,*) + + ' Possible join between contig ',JCONN,' in the ', + + STRAND,' sense and contig ',NCON + WRITE(KBOUT,1000)X +1000 FORMAT(' Percentage mismatch after alignment = ',F4.1) + WRITE(NAME1,1002)JCONN + WRITE(NAME2,1002)NCON + 1002 FORMAT(I6) + CALL FMT4LP(SEQC2(1),SEQG2(IENDG),LO,IENDCT,IENDGT,KBOUT, + + NAME2,NAME1) + END IF + IFAIL=0 + END + SUBROUTINE ADISM5(IDIM,IDIMG,SAVPS,SAVPG,IDSAV, + +CENDS,NENDS,IDCEND,MAXCON,ILEFTS,ILC,IPOSC,IPOSG,ISENSE, + +LLINO,IMATC,ISTRAN,MAXC,KBOUT,MCON) +C AUTHOR: RODGER STADEN + INTEGER ILEFTS(MAXC),ILC(MAXC),IPOSC(MAXC),IPOSG(MAXC) + INTEGER ISENSE(MAXC),LLINO(MAXC),MCON(MAXC) + INTEGER CENDS(MAXCON) + INTEGER NENDS(MAXCON) + INTEGER SAVPS(IDSAV),SAVPG(IDSAV) + NEXTC=IDIM+1 + CALL BUB2AS(SAVPS,SAVPG,IDSAV) + IMATC=IMATC+1 + CALL ADISM6(SAVPS(1),SAVPG(1),CENDS,NENDS,IDCEND,MAXCON, + + ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN,NEXTC,MAXC, + + KBOUT,MCON) + LEND=IDIMG-SAVPG(1)+SAVPS(1) + DO 10 I=2,IDSAV + IF((SAVPS(I).LT.LEND).AND.(SAVPS(I).LT.NEXTC))GO TO 10 + IMATC=IMATC+1 + CALL ADISM6(SAVPS(I),SAVPG(I),CENDS,NENDS,IDCEND,MAXCON, + + ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN,NEXTC,MAXC, + + KBOUT,MCON) + LEND=IDIMG-SAVPG(I)+SAVPS(I) +10 CONTINUE + IMATC = MIN(IMATC,MAXC) + END + SUBROUTINE ADISM6(ISAVPS,SAVPG,CENDS,NENDS, + +IDCEND,MAXCON,ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN, + +NEXTC,MAXC,KBOUT,MCON) +C AUTHOR: RODGER STADEN + INTEGER ILEFTS(MAXC),ILC(MAXC),IPOSC(MAXC),IPOSG(MAXC) + INTEGER ISENSE(MAXC),LLINO(MAXC),MCON(MAXC) + INTEGER SAVPS,SAVPG,CENDS(MAXCON) + INTEGER NENDS(MAXCON) + SAVPS=ISAVPS-19 + JJ=1 + DO 5 J=2,IDCEND + IF(SAVPS.GT.CENDS(J))GO TO 5 + JJ=J-1 + GO TO 6 +5 CONTINUE + JJ=IDCEND +6 CONTINUE +C new bit need to save contig number for alinej + SAVPS=SAVPS-1 + LCL=SAVPS-CENDS(JJ) + LCR=CENDS(JJ+1)-ISAVPS-1 + NEXTC=CENDS(JJ+1)+20 + IF(IMATC.LE.MAXC) THEN + MCON(IMATC) = JJ + ILEFTS(IMATC)=CENDS(JJ)+20 + ILC(IMATC)=LCL+LCR+1 + IPOSC(IMATC)=LCL+1 + IPOSG(IMATC)=SAVPG + LLINO(IMATC)=NENDS(JJ) + ISENSE(IMATC)=1 + IF(ISTRAN.EQ.2)ISENSE(IMATC)=-1 + ELSE + CALL ERROM(KBOUT,'Warning: too many overlaps') + END IF + END +C JCONS + SUBROUTINE JCONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,IDBSIZ,ISTART,ANS,LLINO,LREG,RREG,TEMP, + +CHRSIZ,MAXGL2,KBOUT, + +IDEVW,IFAIL,MAXGEL,IDM,PERCD,TGEL,ILADD,IRADD,MAXCON, + +IWIN,NBAD) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),ANS,CHRSIZ,ILADD(MAXCON),IRADD(MAXCON) + INTEGER LREG,RREG,X,Y,TEMP(CHRSIZ,MAXGL2) + CHARACTER SEQ1(IDIM1) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),TGEL(MAXGEL) + CHARACTER NAMPRO*(*) + CALL BUSY(KBOUT) +C IF(IWIN.GT.0) PERC = REAL(NBAD)/REAL(IWIN) + IEND = 1 + IFAIL=0 + N=IDBSIZ-NCONTS + NCONS = 0 + DO 110 I=N,IDBSIZ-1 + J=LNBR(I) + X=1 + Y=RELPG(I) + ISTART=ISTART+1 + IF((ISTART+19+Y+2*MAXGEL).GT.IDIM1)THEN + WRITE(KBOUT,1009)IDIM1 +1009 FORMAT( + + ' Maximum consensus length(',I6,') exceeded',/, + + ' calculation aborted') + IFAIL=1 + RETURN + END IF + CALL ADDTIT(SEQ1(ISTART),NAMPRO,J,ISTART) + NCONS = NCONS + 1 + IDIN = 0 + IF(IWIN.GT.0) THEN + IDIN = MAXGEL + CALL GETEX(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,I,1, + + GEL,TGEL,IDIN,IWIN,NBAD) + IF(IDIN.GT.0)CALL SQCOPY(GEL,SEQ1(ISTART),IDIN) + END IF + ILADD(NCONS) = IDIN + ISTART = ISTART + IDIN + CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + SEQ1(ISTART),Y,GEL,X,Y,J,IDBSIZ,TEMP,CHRSIZ,MAXGL2,IDEVW,MAXGEL, + + IDM,PERCD) + ISTART=ISTART+Y-1 + IDIN = 0 + IF(IWIN.GT.0) THEN + IDIN = MAXGEL + CALL GETEX(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,I,2, + + GEL,TGEL,IDIN,IWIN,NBAD) + IF(IDIN.GT.0)CALL SQCOPY(GEL,SEQ1(ISTART+1),IDIN) + END IF + IRADD(NCONS) = IDIN + ISTART = ISTART + IDIN +110 CONTINUE + END + SUBROUTINE GETEX(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,ICONT,IEND, + +GEL,GELT,ID,IWIN,NBAD) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ),RELPG(IDBSIZ) + CHARACTER GEL(ID),GELT(ID) + EXTERNAL NOK2 + MAXGEL = ID + IDT = 0 +C routine to find a possible extension to a contig by looking in a tag file +C contig ICONT end IEND = 1 =left 2=right +C return data in GEL, of length ID +C The worst aspect of this is that if we dont choose the very end reading +C we dont know where it lies relative to the consensus. For now just assume +C there are no length difference between the extension and the consensus +C and just add it on the end + IF(IEND.EQ.1) THEN + LMOST = 1 + IGEL = LNBR(ICONT) + 10 CONTINUE + IF(IGEL.EQ.0) GO TO 100 + IF(RELPG(IGEL).GT.MAXGEL) GO TO 100 + IF(LNGTHG(IGEL).LT.0) THEN + ID = MAXGEL + CALL GETEXT(IGEL,GELT,ID,IOK) + IF(IOK.EQ.0) THEN +C look for number of n's here and change id accordingly + K = NOK2(GELT,ID,IWIN,NBAD) + LT = MIN(LMOST,RELPG(IGEL)-K) + IF(LT.LT.LMOST) THEN + IS = RELPG(IGEL) + N = K - IS + 1 + CALL SQCOPY(GELT(IS),GEL,N) + CALL SQREV(GEL,N) + CALL SQCOM(GEL,N) + IDT = N + LMOST = LT + END IF + ELSE +C WRITE(*,*)'COCKUP IN GETEXT, gel',IGEL + END IF + END IF + IGEL = RNBR(IGEL) + GO TO 10 + ELSE + IGEL = RNBR(ICONT) + LMOST = RELPG(ICONT) + IDC = RELPG(ICONT) + 20 CONTINUE + IF(IGEL.EQ.0) GO TO 100 + IF(LMOST-RELPG(IGEL).GT.MAXGEL) GO TO 100 + IF(LNGTHG(IGEL).GT.0) THEN + ID = MAXGEL + CALL GETEXT(IGEL,GELT,ID,IOK) + IF(IOK.EQ.0) THEN + K = NOK2(GELT,ID,IWIN,NBAD) + LT = MAX(LMOST,RELPG(IGEL)+LNGTHG(IGEL)+K-1) + IF(LT.GT.LMOST) THEN + IS = RELPG(ICONT) - (RELPG(IGEL) + LNGTHG(IGEL)) + 2 + N = K - IS + 1 + CALL SQCOPY(GELT(IS),GEL,N) + IDT = N + LMOST = LT + END IF + ELSE +C WRITE(*,*)'COCKUP IN GETEXT, GEL',IGEL + END IF + END IF + IGEL = LNBR(IGEL) + GO TO 20 + END IF + 100 CONTINUE + ID = IDT + END + INTEGER FUNCTION NOK2(GEL,ID,IWIN,NBADIN) + CHARACTER GEL(ID) + PARAMETER (MAXPOS = 101) + INTEGER POSNS(MAXPOS),R + EXTERNAL KWRAP +C count N's over a window of iwin, return position +C when over NBAD +C INIT + NBAD = NBADIN + 1 + I = 0 + N = 0 + NOK2 = ID + L = 1 + R = 0 + IF(NBAD.GT.MAXPOS)THEN + WRITE(*,*)'Scream: nok2 not happy' + RETURN + END IF + 10 CONTINUE + I = I + 1 + IF(I.GT.ID) RETURN + IF(GEL(I).EQ.'-') THEN + N = N + 1 + R = KWRAP(R,NBAD) + POSNS(R) = I + IF(N.GE.NBAD) THEN + IF(POSNS(R)-POSNS(L)+1.LT.IWIN) THEN + NOK2 = POSNS(L) + RETURN + END IF + L = KWRAP(L,NBAD) + END IF + END IF + GO TO 10 + END + INTEGER FUNCTION KWRAP(I,J) + IT = I + 1 + IF(IT.GT.J) IT = 1 + KWRAP = IT + END diff --git a/src/staden/dbsysold.f b/src/staden/dbsysold.f new file mode 100644 index 0000000..6bfa0e8 --- /dev/null +++ b/src/staden/dbsysold.f @@ -0,0 +1,2509 @@ +C dbsys routines that were changed when starting to use .rd files +C these versions are the pre .rd ones and afre used by sap and xsap +C post .rd equivalents are in dbsysnew.f +C 8-4-92 removed prompt for minsli in dbauto +C 22-5-91 added new remove gel reading function. Changed movgel, breakc +C 21-8-91 Added routines to find internal overlaps +C 8-11-91 Fixed bugs in routines to find internal joins +C 2-3-92 Set filnam = ' ' for some call to openf1 +C +C SUBROUTINE TO ENTER NEW GEL SEQUENCES INTO DATA BASE. +C IT READS IN AN ARCHIVE VERSION AND WRITES OUT A WORKING VERSION. +C IT ALSO SETS UP ANY RELATIONSHIPS WITH OTHER DATA IN THE DATABASE +C BOTH BY POSITION IN A CONTIG AND POINTERS TO LEFT AND RIGHT +C NEIGHBOURS. + SUBROUTINE AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,NAMARC,X,ITYPE,ISENSE,SEQC2,ITOTPC, + +IDIM,IDC,NCONTC,LINCON,IFAIL,IDBSIZ,KBOUT,IDEVR,IDEVW,IDEVN, + +MAXGEL) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),X,Y + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),NAMARC*(*) + CHARACTER SEQC2(IDC) + CHARACTER NAMARK*10 +C WRITE(*,*)'X,ITYPE,ISENSE,IDIM,IDC' +C WRITE(*,*)X,ITYPE,ISENSE,IDIM,IDC +C SET FAIL FLAG + IFAIL=0 +C WRITE(KBOUT,1000) +C1000 FORMAT(' TRYING TO ENTER NEW GEL READING INTO DATABASE') +C IS THERE SPACE? + IF((IDBSIZ-(NGELS+NCONTS)).GT.2)GO TO 5 +C FULL + WRITE(KBOUT,1999)IDBSIZ +1999 FORMAT(' Database full, current size=',I6,' Extend with copy') + IFAIL=7 + RETURN +5 CONTINUE +C NEED TO CHECK TO SEE IF GEL ALREADY IN DB +C LOOK THRU ARC FILE + DO 10 J=1,NGELS +C READ(IDEVN,REC=J)NAMARK + CALL READN(IDEVN,J,NAMARK) + IF(NAMARK.NE.NAMARC(1:10))GO TO 10 +C FOUND + WRITE(KBOUT,1013)J +1013 FORMAT(' New gel already in database with number',I6, + +' Entry aborted') + IFAIL=6 + RETURN +10 CONTINUE +C INCREMENT NUMBER OF GELS + NGELS=NGELS+1 +C SET LENGTH THIS GEL + LNGTHG(NGELS)=IDIM*ISENSE +C WRITE NAME OF ARCHIVE TO LIST OF ARCHIVES +C NAMPRO,ARC +C WRITE(IDEVN,REC=NGELS)NAMARC(1:10) + NAMARK=NAMARC(1:10) + CALL WRITEN(IDEVN,NGELS,NAMARK) + WRITE(KBOUT,1003)NGELS +1003 FORMAT(' This gel reading has been given the number ',I6) +C WRITE GEL TO WORKING VERSION + CALL WRITEW(IDEVW,NGELS,GEL,MAXGEL) +C WRITE(IDEVW,REC=NGELS)GEL +C +C +C SET UP RELATIONSHIPS +C DOES THIS GEL OVERLAP? + IF(ITYPE.NE.0)GO TO 100 +C +C DOES NOT OVERLAP SO IT STARTS A CONTIG OF ITS OWN +C SET LEFT AND RIGHT POINTERS TO ZERO,RELPG TO 1 + LNBR(NGELS)=0 + RNBR(NGELS)=0 + RELPG(NGELS)=1 +C WRITE NEW GEL LINE + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) +C WRITE(IDEVR,REC=NGELS)RELPG(NGELS),LNGTHG(NGELS),LNBR(NGELS), +C 1RNBR(NGELS) +C +C SET CONTIG POINTERS AND GENERAL VALUES +C INCREMENT NUMBER OF CONTIGS + NCONTS=NCONTS+1 +C POINTER TO THIS CONTIG + N=IDBSIZ-NCONTS +C POINTER TO LEFT GEL THIS CONTIG + LNBR(N)=NGELS +C POINTER TO RIGHT GEL THIS CONTIG + RNBR(N)=NGELS +C LENGTH OF CONTIG + RELPG(N)=IDIM +C WRITE CONTIG DESCRIPTOR + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) +C WRITE(IDEVR,REC=N)RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) +C WRITE DB DESCRIPTOR + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) +C WRITE(IDEVR,REC=IDBSIZ)NGELS,NCONTS + RETURN +C +100 CONTINUE +C +C +C DOES OVERLAP +150 CONTINUE +C +C LEFT END OR RIGHT OVERLAP? + IF(ITYPE.EQ.1)GO TO 400 +C RIGHT END OR INTERNAL OVERLAP +C +160 CONTINUE +C NEED TO SEARCH THRU THIS CONTIG TO FIND LEFT AND RIGHT +C NEIGHBOURS FOR THIS NEW GEL +C LINE NUMBER OF LEFT END OF CONTIG + N=NCONTC +C LOOK THRU UNTIL CURRENT IS >= THEN IT MUST BE THE PREVIOUS ONE +200 CONTINUE + IF(RELPG(N).GT.X)GO TO 250 +C IS THIS THE LAST GEL IN CONTIG? + IF(RNBR(N).EQ.0)GO TO 350 +C NO SO LOOK AT NEXT + N=RNBR(N) + GO TO 200 +250 CONTINUE +C GEL LIES BETWEEN N AND LNBR(N) +C NEED TO EDIT DB HERE + IF(ITOTPC.GT.0)CALL ABEDIN(RELPG,LNGTHG,LNBR,RNBR, + 1NGELS,NCONTS, + 2GEL,LINCON,X,SEQC2,ITOTPC,IDC,IDBSIZ,KBOUT,IDEVR,IDEVW, + +MAXGEL) +C +C +C SET POINTERS IN NEW GEL + LNBR(NGELS)=LNBR(N) + RNBR(NGELS)=N + RELPG(NGELS)=X +C WRITE NEW GEL LINE + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) +C WRITE(IDEVR,REC=NGELS)RELPG(NGELS),LNGTHG(NGELS),LNBR(NGELS), +C 1RNBR(NGELS) +C SET POINTERS IN LEFT AND RIGHT NEIGHBOURS + K=LNBR(N) + RNBR(K)=NGELS +C RNBR(LNBR(N))=NGELS +C WRITE LEFT AND RIGHT NEIGHBOURS + CALL WRITER(IDEVR,K,RELPG(K),LNGTHG(K), + +LNBR(K),RNBR(K)) +C WRITE(IDEVR,REC=K)RELPG(K),LNGTHG(K),LNBR(K),RNBR(K) + LNBR(N)=NGELS + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) +C WRITE(IDEVR,REC=N)RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) +C WRITE NGELS NCONTS + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) +C WRITE(IDEVR,REC=IDBSIZ)NGELS,NCONTS +C HAVE WE INCREASED LENGTH OF CONTIG? +C ITS LINE NUMBER IS LINCON +C NEED TO UPDATE IDIM IN CASE OF EDITS + IDIM=ABS(LNGTHG(NGELS)) + Y=X+IDIM-1 + IF(Y.LE.RELPG(LINCON))RETURN + RELPG(LINCON)=Y +C WRITE NEW CONTIG LINE + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) +C WRITE(IDEVR,REC=LINCON)RELPG(LINCON),LNGTHG(LINCON),LNBR(LINCON), +C 1RNBR(LINCON) + RETURN +350 CONTINUE +C MUST BE A RIGHT END OVERLAP +C NEED TO EDIT DB HERE + IF(ITOTPC.GT.0)CALL ABEDIN(RELPG,LNGTHG,LNBR,RNBR, + 1NGELS,NCONTS, + 2GEL,LINCON,X,SEQC2,ITOTPC,IDC,IDBSIZ,KBOUT,IDEVR,IDEVW, + +MAXGEL) +C +C +C SET POINTERS FOR NEW GEL + LNBR(NGELS)=N + RNBR(NGELS)=0 + RELPG(NGELS)=X +C WRITE NEW GEL LINE + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) +C WRITE(IDEVR,REC=NGELS) +C 1RELPG(NGELS),LNGTHG(NGELS),LNBR(NGELS),RNBR(NGELS) +C OLD RIGHT END + RNBR(N)=NGELS +C WRITE NEW RIGHT LINE + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) +C WRITE(IDEVR,REC=N)RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) +C +C RESET RIGHT NAME IN CONTIG +C ITS LINE NUMBER IS LINCON + RNBR(LINCON)=NGELS +C HAVE WE INCREASED LENGTH OF CONTIG? +C NEED TO UPDATE LENGTH OF GEL IN CASE OF EDITS + IDIM=ABS(LNGTHG(NGELS)) + Y=X+IDIM-1 + RELPG(LINCON)=MAX(RELPG(LINCON),Y) +C WRITE HERE +C WRITE CONTIG DESCRIPTOR + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) +C WRITE(IDEVR,REC=LINCON)RELPG(LINCON),LNGTHG(LINCON),LNBR(LINCON) +C 1,RNBR(LINCON) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) +C WRITE(IDEVR,REC=IDBSIZ)NGELS,NCONTS + RETURN +C +400 CONTINUE +C +C ADDING TO LEFT END +410 CONTINUE +C NEED TO EDIT DB HERE + IF(ITOTPC.GT.0)CALL ABEDIN(RELPG,LNGTHG,LNBR,RNBR, + 1NGELS,NCONTS, + 2GEL,LINCON,1,SEQC2,ITOTPC,IDC,IDBSIZ,KBOUT,IDEVR,IDEVW, + +MAXGEL) +C +420 CONTINUE +C SET POINTERS IN NEW GEL + RELPG(NGELS)=1 + RNBR(NGELS)=NCONTC + LNBR(NGELS)=0 +C WRITE NEW GEL LINE + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) +C WRITE(IDEVR,REC=NGELS)RELPG(NGELS),LNGTHG(NGELS),LNBR(NGELS), +C 1RNBR(NGELS) +C SET POINTERS IN OLD LEFT END + LNBR(NCONTC)=NGELS + RELPG(NCONTC)=X +C WRITE NEW LEFT END + CALL WRITER(IDEVR,NCONTC,RELPG(NCONTC),LNGTHG(NCONTC), + +LNBR(NCONTC),RNBR(NCONTC)) +C WRITE(IDEVR,REC=NCONTC)RELPG(NCONTC),LNGTHG(NCONTC),LNBR(NCONTC) +C 1,RNBR(NCONTC) +C NEW LENGTH OF CONTIG + RELPG(LINCON)=RELPG(LINCON)+X-1 +C MAY HAVE JUST ADDED A GEL LONGER THAN CONTIG + IDIM=ABS(LNGTHG(NGELS)) + Y=IDIM + IF(Y.GT.RELPG(LINCON))RELPG(LINCON)=Y +C NEW NAME OF LEFT END OF CONTIG + LNBR(LINCON)=NGELS +C WRITE CONTIG DESCRIPTOR + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) +C WRITE(IDEVR,REC=LINCON)RELPG(LINCON),LNGTHG(LINCON),LNBR(LINCON) +C 1,RNBR(LINCON) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) +C WRITE(IDEVR,REC=IDBSIZ)NGELS,NCONTS +C NOW GO THRU AND CHANGE ALL RELATIVE POSITIONS + N=NCONTC +440 CONTINUE + IF(RNBR(N).EQ.0)RETURN + N=RNBR(N) + RELPG(N)=RELPG(N)+X-1 +C WRITE NEW LINE + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) +C WRITE(IDEVR,REC=N)RELPG(N),LNGTHG(N),LNBR(N),RNBR(N) + GO TO 440 + END + SUBROUTINE DBAUTO(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ, + +NGELS,NCONTS,MAXGEL, + +TEMP3,WORDP,WORDN,LPOWRC,POSNS,GELN, + +SEQ1,MAXSEQ,SEQ2,SEQ3,SEQ4,SEQ5,SEQC2,SEQG2,MATCH, + +MAXGLM,MAXGL2,CHRSIZ,ECHRSZ,LENGTH, + +SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON,CONST, + +KBIN,KBOUT,IDEV1,IDEV2,IDEV3,IDEV4,IDEV7,IDEV8,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,NAMARC,NAMPRO,FILE, + +PERCD,IOPEN,IDM,SEQG3,SEQC3,IOK) + INTEGER CHRSIZ,ECHRSZ + INTEGER RELPG(MAXDB),PL(2),PR(2),RMOST + INTEGER LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + INTEGER JOINT(2),ITOTPC(2),ITOTPG(2),IDIM22(2),IDOUT(2) + INTEGER LINCON(2),LLINO(2),ITYPE(2),IFAIL(2) + INTEGER ILEFTS(2),ILC(2),IPOSC(2),IPOSG(2),ISENSE(2) + INTEGER LREG,RREG,X,ANS,ANSJOK + INTEGER TEMP3(ECHRSZ,MAXGL2),CONST(LENGTH) + INTEGER POSNS(MAXSEQ),WORDP(LPOWRC),WORDN(LPOWRC),GELN(MAXGLM) + INTEGER CENDS(MAXCON),NENDS(MAXCON) + CHARACTER SEQ3(MAXGLM),SEQC2(MAXGLM,2),SEQG2(MAXGLM,2) + CHARACTER SEQ1(MAXSEQ),SEQ2(MAXGLM),MATCH(MAXGLM),SEQ4(MAXGLM) + INTEGER SAV1(MAXSAV),SAV2(MAXSAV),SAV3(MAXSAV) + CHARACTER NAMARC*(*),NAMPRO*(*),FILE*(*) + CHARACTER GET,SEQ5(MAXGLM),HELPF*(*),SEQG3(MAXGLM),SEQC3(MAXGLM) + PARAMETER (MAXPRM = 32) + CHARACTER PROMPT(3)*(MAXPRM) + SAVE GET + DATA GET/'>'/ + WRITE(KBOUT,*)' Automatic sequence assembler' + IFAIL(1) = 0 + IEMPTY=0 + IF(NGELS.LT.1)IEMPTY=1 + CALL DBCHEK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +TEMP3,IERR,KBOUT) + IF(IERR.GT.1) RETURN + CALL YESNO(IOKENT,'Permit entry', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOKENT.LT.0) RETURN + CALL YESNO(INF,'Use file of file names', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(INF.LT.0) RETURN + IF(INF.EQ.0) THEN + FILE = ' ' + CALL OPENF1(IDEV7,FILE,0,IOK,KBIN,KBOUT, + + 'File of gel reading names', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + END IF + IF(IOKENT.EQ.0) THEN + FILE = ' ' + CALL OPENF1(IDEV8,FILE,1,IOK,KBIN,KBOUT, + + 'File for names of failures', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + END IF + PROMPT(1) = 'Perform normal shotgun assembly' + PROMPT(2) = 'Put all sequences in one contig' + PROMPT(3) = 'Put all sequences in new contigs' + IOPT = 1 + CALL RADION('Select entry mode',PROMPT,3,IOPT, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOPT.LT.1) RETURN + IF(IOPT.EQ.1) THEN +C parameters for normal assembly + ANSJOK = 0 + CALL YESNO(ANSJOK,'Permit joins', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANSJOK.LT.0) RETURN + MN = LENGTH*2 + MX = MAXGLM + 1 + MINMAT = MAX(15,MN) + CALL GETINT(MN,MX,MINMAT, + +'Minimum initial match', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINMAT = IVAL + MINSLI = 3 + MN = 0 + MX = 25 + MAXPG = 8 + CALL GETINT(MN,MX,MAXPG, + +'Maximum pads per gel', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXPG = IVAL + MN = 0 + MX = 25 + MAXPC = 8 + CALL GETINT(MN,MX,MAXPC, + +'Maximum pads per gel in contig', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXPC = IVAL + IF(IOKENT.EQ.0) THEN + RMN = 0. + RMX = 15. + PERMAX = 8. + CALL GETRL(RMN,RMX,PERMAX, + + 'Maximum percent mismatch after alignment', + + VAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + PERMAX = VAL + END IF + IDIM1=0 + MAXOVR=MAXGEL-3*MAX(MAXPC,MAXPG) + ANS=0 + IF(IEMPTY.EQ.0) + +CALL ACONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,MAXSEQ,SEQ2,IDBSIZ,IDIM1,ANS,KDUMM,KDUMM,KDUMM,TEMP3, + +ECHRSZ,MAXGL2,KBOUT,IDEV2,IFAIL(1),MAXGEL,IDM,PERCD) + END IF + JGEL = 0 + JNGEL = 0 + JNJOIN = 0 + IMATC = 0 + IF(IFAIL(1).NE.0)GO TO 900 +C +C +1 CONTINUE +C +C + IDIM2=MAXGEL + IF(INF.EQ.1) THEN +3 CONTINUE + MN = 0 + CALL GTSTR('Gel reading name',' ',NAMARC,MN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.3) RETURN + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 3 + END IF + ELSE + READ(IDEV7,1002,END=900)NAMARC + END IF +1002 FORMAT( A) + DO 77 MM=1,80 + MATCH(MM)=GET +77 CONTINUE + WRITE(IDEV,1077)(MATCH(KK),KK=1,79) +1077 FORMAT(' ',79A1) + JGEL = JGEL + 1 + WRITE(IDEV,*)'Processing',JGEL,' in batch' +1007 FORMAT(' Gel reading name=',A) + WRITE(IDEV,1007)NAMARC + CALL OPENRS(IDEV4,NAMARC,IOK,LRECL,2) + IF(IOK.NE.0)THEN + IF(INF.EQ.1) RETURN + WRITE(IDEV,*)' Error opening gel reading file' + GO TO 1 + END IF + CALL ARRFIM(IDEV4,SEQ2,IDIM2,KBOUT) + CLOSE(UNIT=IDEV4) + WRITE(IDEV,1800)IDIM2 +1800 FORMAT(' Gel reading length=',I6) + IF(IOPT.NE.1) THEN + CALL DBAUTP(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + SEQ2,NAMARC,JOINT,ITYPE,ISENSE,SEQC2,ITOTPC, + + IDIM2,IDOUT,LLINO,LINCON,IFAIL,IDBSIZ,MAXDB,IDEV, + + IDEV1,IDEV2,IDEV3,MAXGEL,IMATC,IEMPTY,IOPT) + IF(IFAIL(1).NE.0) GO TO 800 + JNGEL = JNGEL + 1 + GO TO 1 + END IF + IF(IDIM2.LT.MINMAT)THEN + WRITE(IDEV,*) + +' Reading shorter than minimum match, so not processed' + GO TO 1 + END IF + CALL SQCOPY(SEQ2,SEQ3,IDIM2) + IFCOMP=0 + IMATC=0 + IF(IEMPTY.EQ.0) + +CALL AUTOCN(SEQ1,IDIM1,SEQ2,IDIM2,ILEFTS,ILC,IPOSC, + +IPOSG,ISENSE,LLINO,IMATC,IFCOMP,MINMAT,POSNS,WORDP,WORDN, + +CONST,LENGTH,LPOWRC,IDEV,MATCH,MAXGEL,MAXGLM,SEQ5,GELN, + +SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON, + +SEQG2,SEQC2,SEQ4,IDOUT,IDIM22,ITOTPG,ITOTPC,JOINT,IFAIL, + +ITYPE,MAXPC,MAXPG,PERMAX,MINSLI,SEQG3,SEQC3,KFAIL) + IF(IOKENT.NE.0) GO TO 1 +C THIS RETURNS THE FOLLOWING: +C ILEFTS POSITION IN CONSENSUS OF LEFT END OF MATCHING CONTIGS +C ILC LENGTHS OF MATCHING CONTIGS +C IPOSC POSITION OF MATCH RELATIVE TO CONTIG +C IPOSG POSITION OF MATCH RELATIVE TO NEW GEL +C ISENSE SENSE OF NEW GEL +C LLINO LEFT GEL NUMBER IN MATCHING CONTIGS +C IMATC THE NUMBER OF MATCHING CONTIGS (>2 IS ERROR!) +C IFCOMP ERROR FLAG FOR COMPARISON (COMPARISON ARRAYS OVERFLOWED) + IF(IFCOMP.NE.0)GO TO 800 + CALL SQCOPY(SEQ3,SEQ2,IDIM2) + IF(IMATC.GT.0)GO TO 200 +C +C NO OVERLAP NEW CONTIG +C +C ITYPE 0 = NO OVERLAP +C ISENSE 1 = SAME SENSE AS ARCHIVE + IF(IFAIL(1).NE.0) GO TO 800 + ITYPE(1)=0 + ISENSE(1)=1 + IDOUT(1)=MAXGEL + WRITE(IDEV,1015) +1015 FORMAT(' New gel reading does not overlap: start a new contig') + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ2,NAMARC,X,ITYPE,ISENSE,SEQC2(1,1),ITOTPC(1), + +IDIM2,IDOUT(1),LLINO,LINCON,IFAIL,IDBSIZ,IDEV, + +IDEV1,IDEV2,IDEV3,MAXGEL) + IF(IFAIL(1).NE.0)GO TO 800 + IEMPTY=0 + IDIM1=IDIM1+1 + IF((IDIM1+19+IDIM2).GT.MAXSEQ)THEN + WRITE(IDEV,1021)MAXSEQ +1021 FORMAT(' Database maximum consensus length (',I6,') exceeded') + GO TO 900 + END IF + CALL ADDTIT(SEQ1(IDIM1),NAMPRO,NGELS,IDIM1) + CALL MSTLKL(SEQ2,IDIM2) + CALL SQCOPY(SEQ2,SEQ1(IDIM1),IDIM2) + IDIM1=IDIM1+IDIM2-1 + JNGEL = JNGEL + 1 + GO TO 1 +C +C +C +200 CONTINUE +C +C +C OVERLAP SO TRY TO ALIGN THE SEQUENCES +C +C + DO 100 I=1,IMATC + N=IDBSIZ-NCONTS + DO 99 J=N,IDBSIZ-1 + IF(LNBR(J).NE.LLINO(I))GO TO 99 + LINCON(I)=J + GO TO 100 +99 CONTINUE + WRITE(IDEV,10077)LLINO(I) +10077 FORMAT(' Contig line for contig',I6,' not found!') + GO TO 800 +100 CONTINUE +C + IF((IMATC.EQ.2).AND.(ANSJOK.EQ.0))GO TO 400 +C +C +C SINGLE OVERLAP +C +C +C + WRITE(IDEV,1014)LLINO(1) +1014 FORMAT(' New gel reading overlaps contig',I6) + IF(ITOTPG(1).GT.0) CALL CCTA(SEQG2(1,1),IDIM22(1)) + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQG2(1,1),NAMARC,JOINT(1),ITYPE(1),ISENSE(1), + +SEQC2(1,1), + +ITOTPC(1),IDIM22(1),IDOUT(1),LLINO(1),LINCON(1), + +IFAIL(1),IDBSIZ,IDEV,IDEV1,IDEV2,IDEV3,MAXGEL) + IF(IFAIL(1).NE.0)GO TO 800 + CALL DELCON(SEQ1,ILEFTS(1),ILC(1),IDIM1) + LREG=1 + RREG=RELPG(LINCON(1)) + IGELC=LNBR(LINCON(1)) + ANS=1 + CALL ACONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,MAXSEQ,SEQ2,IDBSIZ,IDIM1,ANS,IGELC,LREG,RREG,TEMP3, + +ECHRSZ,MAXGL2,IDEV,IDEV2,IFAIL(1),MAXGEL,IDM,PERCD) + IF(IFAIL(1).NE.0)GO TO 900 + JNGEL = JNGEL + 1 + IF(KFAIL.NE.0) GO TO 800 + GO TO 1 +C +C +C DOUBLE OVERLAP +C +C +400 CONTINUE + WRITE(IDEV,1013)LLINO +1013 FORMAT(' Overlap between contigs',I6,' and',I6) + IF(ANSJOK.NE.0)GO TO 800 + IF(LLINO(1).EQ.LLINO(2))THEN + WRITE(IDEV,*)' Trying to form loop in contig',LLINO(1) + WRITE(IDEV,*)' Gel not entered' + GO TO 800 + END IF + CALL AJOIN3(RELPG,IDBSIZ,LINCON,ITYPE,ISENSE,JOINT, + +IDIM22,KLASS,IOVER,IDEV,PL,PR) + IF(IOVER.GT.MAXOVR)THEN + WRITE(IDEV,*)' Overlap too large: entry only' + IFAIL(2)=1 + GO TO 600 + END IF +C WHICH CONTIG IS LEFTMOST? + LMOST=1 + RMOST=2 + IF(PL(1).GT.PL(2))THEN + LMOST=2 + RMOST=1 + END IF +C SAVE LENGTH OF RMOST CONTIG FOR DELETION STEP LATER + ILCR=ILC(RMOST) + IF(ITOTPG(LMOST).GT.0) CALL CCTA(SEQG2(1,LMOST),IDIM22(LMOST)) + WRITE(IDEV,1012)LLINO(LMOST) +1012 FORMAT(' Entering the new gel reading into contig',I6) + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQG2(1,LMOST),NAMARC,JOINT(LMOST),ITYPE(LMOST), + +ISENSE(LMOST),SEQC2(1,LMOST),ITOTPC(LMOST), + +IDIM22(LMOST),IDOUT(LMOST),LLINO(LMOST),LINCON(LMOST), + +IFAIL(LMOST),IDBSIZ,IDEV,IDEV1,IDEV2,IDEV3, + +MAXGEL) + IF(IFAIL(LMOST).NE.0)GO TO 800 + JNGEL = JNGEL + 1 + IF(ITYPE(LMOST).EQ.1)LLINO(LMOST)=NGELS + IF(ILEFTS(LMOST).LT.ILEFTS(RMOST))THEN + ILEFTS(RMOST)=ILEFTS(RMOST)-ILC(LMOST)-20 + END IF + CALL DELCON(SEQ1,ILEFTS(LMOST),ILC(LMOST),IDIM1) + LREG=1 + RREG=RELPG(LINCON(LMOST)) + IGELC=LNBR(LINCON(LMOST)) + ANS=1 + CALL ACONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,MAXSEQ,SEQ2,IDBSIZ,IDIM1,ANS,IGELC,LREG,RREG,TEMP3, + +ECHRSZ,MAXGL2,IDEV,IDEV2,IFAIL(1),MAXGEL,IDM,PERCD) + IF(IFAIL(1).NE.0)GO TO 900 + ILC(LMOST)=RREG + ILEFTS(LMOST)=IDIM1-RREG+1 + DO 500 I=1,2 + IF(ISENSE(I).EQ.-1)THEN + CALL CMPLMT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON(I), + + LLINO(I),SEQ2,IDBSIZ,IDEV,IDEV1,IDEV2,MAXGEL) + CALL SQREV(SEQ1(ILEFTS(I)),ILC(I)) + CALL SQCOM(SEQ1(ILEFTS(I)),ILC(I)) + KT=IDIM1 + CALL ADDTIT(SEQ1((ILEFTS(I)-20)),NAMPRO,LNBR(LINCON(I)),KT) + END IF +500 CONTINUE +C NEED TO KNOW POSITION OF OVERLAP RELATIVE TO CONTIG, TO CONSENSUS +C WHICH BITS TO SEND TO ALIGNMENT ROUTINES +C SET UP FOR ALINE (NOTE RMOST IS EQUIVALENT TO THE GEL READING AND +C SO IS SLID ALONG THE LMOST CONTIG. THE SECTION SENT TO ALINE MUST +C BE OF LENGTH < MAXGEL-2*MAX(MAXPC,MAXPG) +C IT MUST START AT POSITION 1 IN THE RMOST CONTIG AND EXTEND + IPOSC(LMOST)=PL(RMOST)+RELPG(NGELS)-1 + ILCT = RELPG(LINCON(LMOST)) - RELPG(NGELS) - PL(RMOST) + 2 + ILC(RMOST)=MIN(ILCT,ILC(RMOST)) + IPOSC(RMOST)=1 + IDOUT(LMOST)=MAXGEL + IDOUT(RMOST)=MAXGEL + IDSAV=MAXSAV +C ON INPUT TO ALINE ILC(RMOST) CONTAINS THE OVERLAP LENGTH +C ON OUTPUT IT CONTAINS THE LENGTH OF THE ALIGNED SECTION (IE INCLUDING +C PADS) + WRITE(IDEV,1009) +1009 FORMAT(' Trying to align the two contigs') + CALL ALINE(SEQ1(ILEFTS(LMOST)),SEQ1(ILEFTS(RMOST)), + +SEQC2(1,RMOST),SEQC2(1,LMOST),SAV1,SAV2,SAV3,IDSAV, + +ILC(LMOST),ILC(RMOST),IDOUT(LMOST),IPOSC(LMOST),IPOSC(RMOST), + +MINSLI,JOINT(LMOST),ITOTPC(LMOST),ITOTPC(RMOST),IFAIL(1), + +ITYPE(1),MAXPC,MAXPC,PERMAX,IDEV,SEQ4,MAXGEL,Z) +C SEQC2(1,LMOST) NOW CONTAINS THE ALIGNED SECTION OF THE LMOST CONTIG +C SEQC2(1,RMOST) NOW CONTAINS THE ALIGNED SECTION OF THE RMOST CONTIG +C ILC(RMOST) IS NOW THE LENGTH OF ALIGNED SECTION OF THE RMOST CONTIG +C IDOUT(LMOST) IS NOW THE LENGTH OF ALIGNED SECTION OF THE LMOST CONTIG +C JOINT(LMOST) IS THE POSITION OF THE JOIN RLETIVE TO THE LMOST CONTIG +C ITYPE IS TYPE OF OVERLAP (-1 = RIGHT END OR INTERNAL, 1 = LEFT END) +C NB SHOULD ALWAYS BE -1 +C IF THIS HAS BEEN DONE OK WE CAN EDIT THE TWO CONTIGS THEN JOIN + IF(IFAIL(1).NE.0)THEN + WRITE(IDEV,*)' Failed to align the two overlapping contigs' + GO TO 800 + END IF + IF(ITOTPC(LMOST).GT.0)THEN + WRITE(IDEV,1017)LLINO(LMOST) +1017 FORMAT(' Editing contig',I6) + CALL ABEDIN(RELPG,LNGTHG,LNBR,RNBR, + + NGELS,NCONTS,SEQ3,LINCON(LMOST),JOINT(LMOST),SEQC2(1,LMOST), + + ITOTPC(LMOST),IDOUT(LMOST),IDBSIZ,IDEV,IDEV1,IDEV2, + + MAXGEL) + END IF + JOINT(RMOST)=1 + IDOUT(RMOST)=ILC(RMOST) + IF(ITOTPC(RMOST).GT.0)THEN + WRITE(IDEV,1017)LLINO(RMOST) + CALL ABEDIN(RELPG,LNGTHG,LNBR,RNBR, + + NGELS,NCONTS,SEQ3,LINCON(RMOST),JOINT(RMOST),SEQC2(1,RMOST), + + ITOTPC(RMOST),IDOUT(RMOST),IDBSIZ,IDEV,IDEV1,IDEV2, + + MAXGEL) + END IF + ILC(RMOST)=ILCR + LTL=LNBR(LINCON(LMOST)) + LTR=LNBR(LINCON(RMOST)) + WRITE(IDEV,1018)LNBR(LINCON(LMOST)),LNBR(LINCON(RMOST)) +1018 FORMAT(' Completing the join between contigs',I6,' and',I6) + CALL AJOIN2(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +JOINT(LMOST),LTL,LTR,LINCON(LMOST),LINCON(RMOST),IDEV1) + LLINO(1)=LTL + IF(ILEFTS(LMOST).GT.ILEFTS(RMOST))THEN + CALL DELCON(SEQ1,ILEFTS(LMOST),ILC(LMOST),IDIM1) + CALL DELCON(SEQ1,ILEFTS(RMOST),ILC(RMOST),IDIM1) + END IF + IF(ILEFTS(RMOST).GE.ILEFTS(LMOST))THEN + CALL DELCON(SEQ1,ILEFTS(RMOST),ILC(RMOST),IDIM1) + CALL DELCON(SEQ1,ILEFTS(LMOST),ILC(LMOST),IDIM1) + END IF + LREG=1 + RREG=JOINT(LMOST) + IGELC=LLINO(1) + ANS=1 + CALL ACONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,MAXSEQ,SEQ2,IDBSIZ,IDIM1,ANS,IGELC,LREG,RREG,TEMP3, + +ECHRSZ,MAXGL2,IDEV,IDEV2,IFAIL(1),MAXGEL,IDM,PERCD) + IF(IFAIL(1).NE.0)GO TO 900 +C CALL FMTDB(SEQ1,IDIM1,1,IDIM1,60,IDEV) + JNJOIN = JNJOIN + 1 + IF(KFAIL.NE.0) GO TO 800 + GO TO 1 +C +C +C JOINS THAT FAIL INITIAL ALIGNMENT CRITERIA +C +C +600 CONTINUE +C COME HERE FOR JOINS THAT FAIL: WE MIGHT BE ABLE TO ENTER THE GEL +C INTO A SINGLE CONTIG + IGOOD=0 + IF(IFAIL(1).EQ.0)IGOOD=1 + IF(IFAIL(2).EQ.0)IGOOD=2 + IF(IGOOD.EQ.0)GO TO 800 + IF(ITOTPG(IGOOD).GT.0) CALL CCTA(SEQG2(1,IGOOD),IDIM22(IGOOD)) + WRITE(IDEV,1012)LLINO(IGOOD) + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQG2(1,IGOOD),NAMARC,JOINT(IGOOD),ITYPE(IGOOD), + +ISENSE(IGOOD),SEQC2(1,IGOOD),ITOTPC(IGOOD), + +IDIM22(IGOOD),IDOUT(IGOOD),LLINO(IGOOD),LINCON(IGOOD), + +IFAIL(IGOOD),IDBSIZ,IDEV,IDEV1,IDEV2,IDEV3, + +MAXGEL) + IF(IFAIL(IGOOD).NE.0)GO TO 800 + JNGEL = JNGEL + 1 + CALL DELCON(SEQ1,ILEFTS(IGOOD),ILC(IGOOD),IDIM1) + ANS=1 + IGELC=LNBR(LINCON(IGOOD)) + LREG=1 + RREG=RELPG(LINCON(IGOOD)) + CALL ACONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,MAXSEQ,SEQ2,IDBSIZ,IDIM1,ANS,IGELC,LREG,RREG,TEMP3, + +ECHRSZ,MAXGL2,IDEV,IDEV2,IFAIL(1),MAXGEL,IDM,PERCD) + IF(IFAIL(1).NE.0)GO TO 900 + WRITE(IDEV,1020)LLINO,LLINO(IGOOD) +1020 FORMAT(' Could not join contigs',I4,' and',I4,' but the gel', + +' has been entered into contig',I4,/, + +' If required do the join manually.') +C +C +C ALL FAILURES +C +C +C +800 CONTINUE + WRITE(IDEV,10888) +10888 FORMAT(' Failed gel name written to error file') + WRITE(IDEV8,1002)NAMARC + GO TO 1 +900 CONTINUE + WRITE(KBOUT,*)'Batch finished' + WRITE(KBOUT,*)JGEL,' sequences processed' + WRITE(KBOUT,*)JNGEL,' sequences entered into database' + WRITE(KBOUT,*)JNJOIN,' joins made' + RETURN + END + SUBROUTINE DBAUTP(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ2,NAMARC,JOINT,ITYPE,ISENSE,SEQC2,ITOTPC, + +IDIM2,IDOUT,LLINO,LINCON,IFAIL,IDBSIZ,MAXDB,IDEV, + +IDEV1,IDEV2,IDEV3,MAXGEL,IMATC,IEMPTY,IOPT) + INTEGER RELPG(MAXDB) + INTEGER LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER SEQ2(MAXGEL),SEQC2(MAXGEL) + CHARACTER NAMARC*(*) +C deals with entering all readings into contig 1 (IOPT=2) +C or all readings into new contigs (IOPT=3) + IF(IOPT.EQ.2) THEN + IF(IMATC.EQ.0) THEN + ITYPE=0 + ISENSE=1 + IDOUT=MAXGEL + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + SEQ2,NAMARC,JOINT,ITYPE,ISENSE,SEQC2,ITOTPC, + + IDIM2,IDOUT,LLINO,LINCON,IFAIL,IDBSIZ,IDEV, + + IDEV1,IDEV2,IDEV3,MAXGEL) + IF(IFAIL.NE.0) RETURN + IEMPTY=0 + IMATC = 1 + ELSE + ITYPE= - 1 + ISENSE=1 + JOINT = 1 + LLINO = 1 + LINCON = IDBSIZ - 1 + ITOTPC = 0 + IDOUT=MAXGEL + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + SEQ2,NAMARC,JOINT,ITYPE,ISENSE,SEQC2,ITOTPC, + + IDIM2,IDOUT,LLINO,LINCON,IFAIL,IDBSIZ,IDEV, + + IDEV1,IDEV2,IDEV3,MAXGEL) + IF(IFAIL.NE.0) RETURN + END IF + ELSE IF(IOPT.EQ.3) THEN + ITYPE=0 + ISENSE=1 + IDOUT=MAXGEL + CALL AENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + SEQ2,NAMARC,JOINT,ITYPE,ISENSE,SEQC2,ITOTPC, + + IDIM2,IDOUT,LLINO,LINCON,IFAIL,IDBSIZ,IDEV, + + IDEV1,IDEV2,IDEV3,MAXGEL) + IF(IFAIL.NE.0) RETURN + END IF + END + SUBROUTINE DBCOPY(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,GEL, + +NAMPRO,IDEV,IDBSIZ,IERR,KBIN,KBOUT,IDEVR,IDEVW,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH,MAXGEL,MAXDB,IDM) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER NAMPRO*(*),NAMARC*10,GEL(MAXGEL) + CHARACTER V2,V1 + INTEGER IWORD,DELDB + PARAMETER (IWORD=4) + PARAMETER (MAXPRM = 21) + CHARACTER PERR(2)*(MAXPRM) + EXTERNAL DELDB + IERR=1 +1 CONTINUE + L = 1 + V1='1' + CALL GTSTR('Make version',V1,V2,L,KBOUT,KBIN,INFLAG) + CALL CCASE(V2,1) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(L.LT.1) V2 = V1 + MN = NGELS + NCONTS + 1 + MX = MAXDB + NEWSIZ = IDBSIZ + CALL GETINT(MN,MX,NEWSIZ, + +'New database size', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CALL BUSY(KBOUT) + NEWSIZ = IVAL +C WHERE SHOULD CHARS BE CHANGED ? + LLL = INDEX(NAMPRO,'.') + 1 + NAMPRO(LLL:)='RL'//V2 + CALL OPENRS(IDEV,NAMPRO,IOK,4,3) + IF(IOK.NE.0) THEN +C problem opening file + IF(IOK.EQ.2) THEN + CALL ERROM(KBOUT,'File already exists') + PERR(1) = 'Retype version number' + PERR(2) = 'Replace database' + IDO = 1 + CALL RADION('Select action',PERR,2,IDO, + + IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(IDO.LT.1) RETURN + IF(IDO.EQ.1) GO TO 1 + IF(IDO.EQ.2) THEN + IOK = DELDB(NAMPRO,V2,IDEV,MAXGEL) + IF(IOK.EQ.0) THEN + LLL = INDEX(NAMPRO,'.') + 1 + NAMPRO(LLL:)='RL'//V2 + CALL OPENRS(IDEV,NAMPRO,IOK,4,3) + IF(IOK.EQ.0) GO TO 2 + ELSE + CALL ERROM(KBOUT,'File delete failed') + END IF + END IF + END IF + GO TO 100 + END IF + 2 CONTINUE + CALL WRITER(IDEV,NEWSIZ,NGELS,NCONTS,NGELS,NCONTS) + DO 10 I=1,NGELS + CALL WRITER(IDEV,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) +10 CONTINUE + M=NEWSIZ-NCONTS + N=IDBSIZ-NCONTS + DO 15 I=N,IDBSIZ-1 + CALL WRITER(IDEV,M,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + M=M+1 +15 CONTINUE + CLOSE(UNIT=IDEV) +C DO SEQUENCES + NAMPRO(LLL:)='SQ'//V2 + IREC=MAXGEL/IWORD + IF(MOD(MAXGEL,IWORD).NE.0)IREC=IREC+1 + CALL OPENRS(IDEV,NAMPRO,IOK,IREC,3) + IF(IOK.NE.0)GO TO 100 +C +C MAKE SURE FILE IS NEWSIZ LINES LONG! + CALL WRITEW(IDEV,NEWSIZ,GEL,MAXGEL) + DO 20 I=1,NGELS + CALL READW(IDEVW,I,GEL,MAXGEL) + CALL WRITEW(IDEV,I,GEL,MAXGEL) +20 CONTINUE + CLOSE(UNIT=IDEV) +C DO ARCHIVE NAMES + NAMPRO(LLL:)='AR'//V2 + CALL OPENRS(IDEV,NAMPRO,IOK,3,3) + IF(IOK.NE.0)GO TO 100 + DO 30 I=1,NGELS + CALL READN(IDEVN,I,NAMARC) + CALL WRITEN(IDEV,I,NAMARC) +30 CONTINUE +C MAKE SURE FILE IS 1000 LINES LONG +C ******** PROBLEMS HERE WRITING INTEGER TO NAMES FILE + WRITE(IDEV,REC=MAXDB)NEWSIZ,MAXGEL,IDM + CLOSE(UNIT=IDEV) + IERR=0 + RETURN +100 CONTINUE + WRITE(KBOUT,9999) +9999 FORMAT(' Error opening new database, copy aborted') + RETURN + END + INTEGER FUNCTION DELDB(NAMPRO,VERSN,IDEV,MAXGEL) + CHARACTER NAMPRO*(*),VERSN + INTEGER DELF + EXTERNAL DELF +C +C delete an xdap database +C +C assume relationships are 4 words, names are 3 and seqs are maxgel +C all recls in BYTES +C + DELDB = 1 + LLL = INDEX(NAMPRO,'.') + 1 + NAMPRO(LLL:)='RL'//VERSN + IF(DELF(NAMPRO,IDEV,16,4).NE.0) RETURN + NAMPRO(LLL:)='AR'//VERSN + IF(DELF(NAMPRO,IDEV,12,4).NE.0) RETURN + NAMPRO(LLL:)='SQ'//VERSN + IF(DELF(NAMPRO,IDEV,MAXGEL,4).NE.0) RETURN + DELDB = 0 + END + SUBROUTINE DBFIX(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,GEL2,IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW,IDEVN, + +IHELPS,IHELPE,IHELP1,IHELP2,FILEH,IDEVH,MAXGEL) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),X + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + CHARACTER NAME*10,NEWNAM*10 + PARAMETER (MAXPRM = 32) + CHARACTER PROMPT(8)*(MAXPRM) + INTEGER L,M,N + CHARACTER GEL2(MAXGEL) + INTEGER GCLIN,CHAINL + EXTERNAL GCLIN,CHAINL + WRITE(KBOUT,1000) +1000 FORMAT( + +' Warning:', + +' make a copy first, and check logical consistency after use') +10 CONTINUE +C +C SELECT OPTION +C CALL BELL(1,KBOUT) +C CALL DBMENU(4,NOPT,IHELPS,IHELPE,FILEH,IDEVH, +C +KBIN,KBOUT) + PROMPT(1) = 'Line change' + PROMPT(2) = 'Edit gel reading' + PROMPT(3) = 'Delete contig line' + PROMPT(4) = 'Shift' + PROMPT(5) = 'Move gel reading' + PROMPT(6) = 'Rename gel reading' + PROMPT(7) = 'Break a contig' + PROMPT(8) = 'Remove a gel reading' + NOPT = 1 + CALL RADION('Alter relationships', PROMPT, 8, NOPT, IHELPS, + + IHELPE, FILEH, IDEVH, KBIN, KBOUT) + IF(NOPT.LT.1)RETURN + IF(NOPT.EQ.-1)RETURN + IF(NOPT.EQ.1)THEN +C LINE CHANGE + MN = 0 + MX = IDBSIZ + LNO = 0 + CALL GETINT(MN,MX,LNO, + + 'Number of line to change', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + IF(IVAL.EQ.0) GO TO 10 + LNO = IVAL + IF(LNO.EQ.IDBSIZ)GO TO 19 + WRITE(KBOUT,*)'Current line' + WRITE(KBOUT,1001)RELPG(LNO),LNGTHG(LNO),LNBR(LNO),RNBR(LNO) +1001 FORMAT(' ',4I6) + MN = 0 + MX = 99999 + X = RELPG(LNO) + CALL GETINT(MN,MX,X, + + 'Relative position', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + X = IVAL + MN = -MAXGEL + MX = 99999 + L = LNGTHG(LNO) + CALL GETINT(MN,MX,L, + + 'Length', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + L = IVAL + MN = 0 + MX = IDBSIZ + M = LNBR(LNO) + CALL GETINT(MN,MX,M, + + 'Left neighbour', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + M = IVAL + MN = 0 + MX = IDBSIZ + N = RNBR(LNO) + CALL GETINT(MN,MX,N, + + 'Right neighbour', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + N = IVAL + CALL WRITER(IDEVR,LNO,X,L,M,N) + RELPG(LNO)=X + LNGTHG(LNO)=L + LNBR(LNO)=M + RNBR(LNO)=N + GO TO 10 +19 CONTINUE +C NCONTS NGELS LINES + MN = 0 + MX = IDBSIZ + LL = NGELS + CALL GETINT(MN,MX,LL, + + 'Number of gel readings', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + LL = IVAL + MN = 0 + MX = IDBSIZ + MM = NCONTS + CALL GETINT(MN,MX,MM, + + 'Number of contigs', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + MM = IVAL + CALL WRITER(IDEVR,IDBSIZ,LL,MM,LL,MM) + NGELS=LL + NCONTS=MM + GO TO 10 + END IF +C +C + IF(NOPT.EQ.4)THEN + MN = 0 + MX = NGELS + LNO = 0 + CALL GETINT(MN,MX,LNO, + + 'Number of first gel reading to shift', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + IF(IVAL.LT.1) GO TO 10 + LNO = IVAL + I = CHAINL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,LNO) + IF(I.EQ.0)THEN + WRITE(KBOUT,*) + + 'Problem with this gel reading. Check logical consistency' + WRITE(KBOUT,*)'of database. Shift not done' + GO TO 10 + END IF + NCONTO = GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,I) + IF(NCONTO.EQ.0)THEN + WRITE(KBOUT,*) + + 'No contig line for this contig. Check logical' + WRITE(KBOUT,*)'consistency of database. Shift not done' + GO TO 10 + END IF + MN = 1 - RELPG(LNO) + MX = RELPG(NCONTO) - RELPG(LNO) + X = MN + CALL GETINT(MN,MX,X, + + 'Distance to shift', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + X = IVAL + CALL SHIFTC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDEVR, + + IDBSIZ,LNO,NCONTO,X) + WRITE(KBOUT,*)'Shift complete' + GO TO 10 + END IF +C + IF(NOPT.EQ.2)THEN + MN = 0 + MX = NGELS + LNO = 0 + CALL GETINT(MN,MX,LNO, + + 'Number of gel reading to edit', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + IF(IVAL.LT.1) GO TO 10 + LNO = IVAL + IDIM=ABS(LNGTHG(LNO)) +C ALLOW EDITING TO BE RELATIVE TO CONTIG POSITION + ISTART=RELPG(LNO) + CALL READW(IDEVW,LNO,GEL,MAXGEL) + CALL EDITGL(GEL,GEL2,IDIM,KBIN,KBOUT,MAXGEL, + + IHELP1,IHELP2,FILEH,IDEVH,ISTART) + CALL WRITEW(IDEVW,LNO,GEL,MAXGEL) + LNGTHG(LNO)=SIGN(IDIM,LNGTHG(LNO)) + CALL WRITER(IDEVR,LNO,RELPG(LNO),LNGTHG(LNO), + + LNBR(LNO),RNBR(LNO)) + GO TO 10 + END IF + IF(NOPT.EQ.3)THEN + MN=IDBSIZ-NCONTS + MX = IDBSIZ - 1 + LNO = 0 + CALL GETINT(MN,MX,LNO, + + 'Number of contig line to delete', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + LNO = IVAL + CALL REMCNL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + LNO,IDEVR) + GO TO 10 + END IF + IF(NOPT.EQ.6)THEN + MN = 0 + MX = NGELS + LNO = 0 + CALL GETINT(MN,MX,LNO, + + 'Number of gel reading to rename', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + IF(IVAL.LT.1) GO TO 10 + LNO = IVAL + CALL READN(IDEVN,LNO,NAME) + L = 10 + CALL GTSTR('name for gel reading', + + NAME,NEWNAM,L,KBOUT,KBIN,INFLAG) + IF(L.GT.0)CALL WRITEN(IDEVN,LNO,NEWNAM) + GO TO 10 + END IF + IF(NOPT.EQ.5)THEN + MN = 0 + MX = NGELS + IFROM = 0 + CALL GETINT(MN,MX,IFROM, + + 'Number of gel reading to move', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + IF(IVAL.LT.1) GO TO 10 + IFROM = IVAL + MN = 0 + MX = NGELS + ITO = 0 + CALL GETINT(MN,MX,ITO, + + 'New number for gel reading', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + IF(IVAL.LT.1) GO TO 10 + ITO = IVAL + CALL MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + GEL,IFROM,ITO,IDEVR,IDEVW,IDEVN,MAXGEL,KBOUT) + GO TO 10 + END IF + IF(NOPT.EQ.7)THEN + CALL BREAKC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,KBIN,KBOUT,IDEVR,IDEVW,IDEVN, + + IHELPS,IHELPE,IHELP1,IHELP2,FILEH,IDEVH,IOK) + GO TO 10 + END IF + IF(NOPT.EQ.8) THEN + CALL REMGD(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + KBIN,KBOUT,GEL,MAXGEL,IDEVR,IDEVW,IDEVN, + + IHELPS,IHELPE,FILEH,IDEVH,IOK) + GO TO 10 + END IF + GO TO 10 + END + SUBROUTINE REMGD(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +KBIN,KBOUT,GEL,MAXGEL,IDEVR,IDEVW,IDEVN, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER HELPF*(*),GEL(MAXGEL) + INTEGER REMME,GCLIN,CHAINL + EXTERNAL GCLIN,CHAINL +C assumes db is logical consistent + WRITE(KBOUT,*)'Remove reading from database' + REMME = NGELS + CALL GETINT(1,NGELS,REMME, + +'Number of reading to remove', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + REMME = IVAL + I = CHAINL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,REMME) + ICONT = GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ,I) + IF(ICONT.EQ.0) THEN + WRITE(KBOUT,*)'No contig line for this reading' + IOK = 1 + RETURN + END IF + CALL REMGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +REMME,ICONT,KBOUT,GEL,MAXGEL,IDEVR,IDEVW,IDEVN) + END + SUBROUTINE REMGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +REMME,ICONT,KBOUT,GEL,MAXGEL,IDEVR,IDEVW,IDEVN) +C Routine to remove a reading from a database +C Cases: 1 left end +C 2 right end +C 3 internal and dispensible +C 4 internal and indispensible +C if 1 change contig lnbr, contig length, lnbr of rnbr of remme, relpgs +C if 2 change contig rnbr, contig length, rnbr of lnbr of remme +C if 3 change contig length, lnbr of rnbr of remme rnbr of lnbr of remme +C if 4 need to break contig, then as for 1 +C if 1 and 2 then also remove contig line +C for all cases move gel ngels to remme (if remme/=ngels) +C and update line idbsiz +C + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER REMME,CLEN + LOGICAL LEFTE,RIGHTE,DISPEN + CHARACTER GEL(MAXGEL) + EXTERNAL CLEN + LEFTE = .FALSE. + RIGHTE = .FALSE. + DISPEN = .FALSE. +C +C Left end ? +C + IF(LNBR(REMME).EQ.0) LEFTE = .TRUE. +C +C Right end ? +C + IF(RNBR(REMME).EQ.0) RIGHTE = .TRUE. +C +C If both true remove the contig line, then overwrite the gel +C + IF(LEFTE.AND.RIGHTE) THEN + WRITE(KBOUT,*)'Removing reading and contig' + IFROM = NGELS + NGELS = NGELS - 1 + CALL REMCNL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + ICONT,IDEVR) + IF(REMME.NE.IFROM) THEN + WRITE(KBOUT,*)'Renumbering reading',IFROM,' to',REMME + CALL MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,GEL,IFROM,REMME,IDEVR,IDEVW,IDEVN,MAXGEL,KBOUT) + END IF + ELSE IF(LEFTE) THEN + WRITE(KBOUT,*)'Removing reading from left end of contig' + LNBR(ICONT) = RNBR(REMME) + I = 1 - RELPG(RNBR(REMME)) + WRITE(KBOUT,*)'Shifting readings in contig by distance=',I + CALL SHIFTC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDEVR, + + IDBSIZ,RNBR(REMME),ICONT,I) + I = LNBR(ICONT) + LNBR(I) = 0 + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + IFROM = NGELS + IF(REMME.NE.IFROM) THEN + WRITE(KBOUT,*)'Renumbering reading',IFROM,' to',REMME + CALL MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,GEL,IFROM,REMME,IDEVR,IDEVW,IDEVN,MAXGEL,KBOUT) + END IF + NGELS = NGELS - 1 + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + ELSE IF(RIGHTE) THEN + WRITE(KBOUT,*)'Removing reading from right end of contig' + RNBR(ICONT) = LNBR(REMME) + I = RNBR(ICONT) + RNBR(I) = 0 + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + RELPG(ICONT) = CLEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,LNBR(ICONT)) + CALL WRITER(IDEVR,ICONT,RELPG(ICONT),LNGTHG(ICONT), + + LNBR(ICONT),RNBR(ICONT)) + IFROM = NGELS + IF(REMME.NE.IFROM) THEN + WRITE(KBOUT,*)'Renumbering reading',IFROM,' to',REMME + CALL MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,GEL,IFROM,REMME,IDEVR,IDEVW,IDEVN,MAXGEL,KBOUT) + END IF + NGELS = NGELS - 1 + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + ELSE +C Is remme indispensible ? + NSTART = RELPG(RNBR(REMME)) + I = REMME +10 CONTINUE + I = LNBR(I) + IF(I.NE.0) THEN + IF((RELPG(I)+ABS(LNGTHG(I))-1).LT.NSTART) GO TO 10 + DISPEN = .TRUE. + END IF + IF(DISPEN) THEN + WRITE(KBOUT,*) + + 'Removing dispensible reading from middle of contig' + I = LNBR(REMME) + RNBR(I) = RNBR(REMME) + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + I = RNBR(REMME) + LNBR(I) = LNBR(REMME) + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + IFROM = NGELS + IF(REMME.NE.IFROM) THEN + WRITE(KBOUT,*)'Renumbering reading',IFROM,' to',REMME + CALL MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,GEL,IFROM,REMME,IDEVR,IDEVW,IDEVN, + + MAXGEL,KBOUT) + END IF + NGELS = NGELS - 1 + RELPG(ICONT) = CLEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,LNBR(ICONT)) + CALL WRITER(IDEVR,ICONT,RELPG(ICONT),LNGTHG(ICONT), + + LNBR(ICONT),RNBR(ICONT)) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + ELSE + WRITE(KBOUT,*) + + 'Removing indispensible reading from middle of contig' + WRITE(KBOUT,*)'So breaking contig first' + IR = REMME + IL = LNBR(REMME) + ILO = LNBR(ICONT) + NCONTO = ICONT + NCONTR = IDBSIZ - NCONTS - 1 + CALL CBREAK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + KBOUT,IDEVR,IDEVW,IDEVN,IR,IL,ILO,NCONTO,NCONTR,IOK) + IF(IOK.NE.0) RETURN + WRITE(KBOUT,*)'Removing reading from left end of contig' + ICONT = IDBSIZ - NCONTS + LNBR(ICONT) = RNBR(REMME) + I = 1 - RELPG(RNBR(REMME)) + WRITE(KBOUT,*)'Shifting readings in contig by distance=',I + CALL SHIFTC(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDEVR, + + IDBSIZ,RNBR(REMME),ICONT,I) + I = LNBR(ICONT) + LNBR(I) = 0 + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + IFROM = NGELS + IF(REMME.NE.IFROM) THEN + WRITE(KBOUT,*)'Renumbering reading',IFROM,' to',REMME + CALL MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,GEL,IFROM,REMME,IDEVR,IDEVW,IDEVN, + + MAXGEL,KBOUT) + END IF + NGELS = NGELS - 1 + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + END IF + END IF + END + SUBROUTINE REMCNL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +REMME,IDEVR) + INTEGER RELPG(IDBSIZ),LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + INTEGER REMME +C Routine to remove a contig line from a db +C Loop deals with case of remove top contig +C Move down all lines from above + DO 10 I = REMME,IDBSIZ-NCONTS+1,-1 + RELPG(I) = RELPG(I-1) + LNGTHG(I) = LNGTHG(I-1) + LNBR(I) = LNBR(I-1) + RNBR(I) = RNBR(I-1) + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) +10 CONTINUE + NCONTS = NCONTS - 1 + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + END + SUBROUTINE MOVGEL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +GEL,FROM,TO,IDEVR,IDEVW,IDEVN,MAXGEL,KBOUT) +C Subroutine to move a gel from line from to line to +C Extended 22-5-91 +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),FROM,TO + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER NAMGEL*10,GEL(MAXGEL) + INTEGER GCLIN,CHAINL + LOGICAL LEFTE,RIGHTE + EXTERNAL GCLIN,CHAINL + LEFTE = .FALSE. + RIGHTE = .FALSE. +C +C left end ? +C + IF(LNBR(FROM).EQ.0) LEFTE = .TRUE. +C +C right end ? +C + IF(RNBR(FROM).EQ.0) RIGHTE = .TRUE. +C +C if both true remove the contig line, then overwrite the gel +C + IF(LEFTE.AND.RIGHTE) THEN + NCONTO = GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,FROM) + IF(NCONTO.EQ.0)THEN + WRITE(KBOUT,*) + + 'This gel has no left neighbour but does not' + WRITE(KBOUT,*)'appear in a contig line!' + ELSE + LNBR(NCONTO) = TO + RNBR(NCONTO) = TO + CALL WRITER(IDEVR,NCONTO,RELPG(NCONTO),LNGTHG(NCONTO), + + LNBR(NCONTO),RNBR(NCONTO)) + END IF + ELSE IF(LEFTE) THEN + NCONTO = GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,FROM) + IF(NCONTO.EQ.0)THEN + WRITE(KBOUT,*) + + 'This gel has no left neighbour but does not' + WRITE(KBOUT,*)'appear in a contig line!' + ELSE + LNBR(NCONTO) = TO + CALL WRITER(IDEVR,NCONTO,RELPG(NCONTO),LNGTHG(NCONTO), + + LNBR(NCONTO),RNBR(NCONTO)) + END IF + ELSE IF(RIGHTE) THEN + I = CHAINL(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,FROM) + NCONTO = GCLIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,I) + IF(NCONTO.EQ.0)THEN + WRITE(KBOUT,*) + + 'This gel has no right neighbour and does not' + WRITE(KBOUT,*)'appear in a contig!' + ELSE + IF(RNBR(NCONTO).NE.FROM)THEN + WRITE(KBOUT,*) + + 'This gel has no right neighbour but does not' + WRITE(KBOUT,*)'appear in a contig line!' + ELSE + RNBR(NCONTO) = TO + CALL WRITER(IDEVR,NCONTO,RELPG(NCONTO),LNGTHG(NCONTO), + + LNBR(NCONTO),RNBR(NCONTO)) + END IF + END IF + END IF + RELPG(TO)=RELPG(FROM) + LNGTHG(TO)=LNGTHG(FROM) + LNBR(TO)=LNBR(FROM) + RNBR(TO)=RNBR(FROM) + CALL READW(IDEVW,FROM,GEL,MAXGEL) + CALL WRITEW(IDEVW,TO,GEL,MAXGEL) + CALL READN(IDEVN,FROM,NAMGEL) + CALL WRITEN(IDEVN,TO,NAMGEL) + CALL WRITER(IDEVR,TO,RELPG(TO),LNGTHG(TO), + +LNBR(TO),RNBR(TO)) +C Do neighbours + IF(LNBR(FROM).NE.0) THEN + I=LNBR(FROM) + RNBR(I)=TO + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + END IF + IF(RNBR(FROM).NE.0) THEN + I=RNBR(FROM) + LNBR(I)=TO + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I), + + LNBR(I),RNBR(I)) + END IF + END + SUBROUTINE DBOPEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,NAMPRO,GEL, + +IDBSIS,IDBSIZ,IERR,KBIN,KBOUT,IDEVR,IDEVW,IDEVN,MAXGEL,MAXGLM, + +LLINO, + +IDM,IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER GEL(MAXGLM) + INTEGER RELPG(IDBSIS) + INTEGER LNGTHG(IDBSIS),LNBR(IDBSIS),RNBR(IDBSIS) + CHARACTER NAMPRO*(*),COPYNO*4,HELPF*(*) + INTEGER IWORD,ANS + PARAMETER (IWORD=4) +C NOTE THIS IS THE MACHINES WORD LENGTH IE HOW MANY CHARS PER WORD + CALL FILLI(RELPG,IDBSIS,0) + CALL FILLI(LNGTHG,IDBSIS,0) + CALL FILLI(LNBR,IDBSIS,0) + CALL FILLI(RNBR,IDBSIS,0) + NAMPRO(1:)=' ' + IERR=1 +1 CONTINUE + L = 0 + CALL GTSTR('Project name',' ',NAMPRO,L,KBOUT,KBIN,INFLAG) + IF(L.LT.1) RETURN + LL = L + CALL CCASE(NAMPRO,1) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + L = 1 + CALL GTSTR('Version','0',COPYNO,L,KBOUT,KBIN,INFLAG) + CALL CCASE(COPYNO,1) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(L.LT.1) COPYNO(1:1) = '0' +C GET .ARN FIRST AS IT CONTAINS LENGTH OF OTHER FILES + NAMPRO(LL+1:LL+1)='.' + NAMPRO(LL+2:LL+2)='A' + NAMPRO(LL+3:LL+3)='R' + NAMPRO(LL+4:LL+4)=COPYNO(1:1) + CALL OPENRS(IDEVN,NAMPRO,IOK,3,4) + IF(IOK.NE.0)GO TO 100 +C ******* PROBLEM HERE READING RECORD 1000 AND INTEGER FROM IDEVN!!! + READ(IDEVN,REC=IDBSIS,ERR=50)IDBSIZ,MAXGEL,IDM + IF(MAXGEL.LT.512)GO TO 50 + IF(MAXGEL.GT.MAXGLM)GO TO 50 + IF(MOD(MAXGEL,512).NE.0)GO TO 50 +20 CONTINUE + IF((IDM.NE.5).AND.(IDM.NE.26))GO TO 51 +21 CONTINUE + NAMPRO(LL+2:LL+2)='R' + NAMPRO(LL+3:LL+3)='L' + CALL OPENRS(IDEVR,NAMPRO,IOK,4,4) + IF(IOK.NE.0)GO TO 100 + NAMPRO(LL+2:LL+2)='S' + NAMPRO(LL+3:LL+3)='Q' +C DEFINE RECORD LENGTH IN TERMS OF NUMBER OF CHARS PER WORD (4 ON VAX) +C AND MAXGEL SIZE + IREC=MAXGEL/IWORD + IF(MOD(MAXGEL,IWORD).NE.0)IREC=IREC+1 + CALL OPENRS(IDEVW,NAMPRO,IOK,IREC,4) + IF(IOK.NE.0)GO TO 100 +C READ A LINE FOR LUCK + CALL READW(IDEVW,1,GEL,MAXGEL) + CALL READR(IDEVR,IDBSIZ,NGELS,NCONTS,IDUM1,IDUM2) + WRITE(KBOUT,10011)NGELS,NCONTS,IDBSIZ,MAXGEL +10011 FORMAT(' Number of gel readings=',I3,' Number of contigs=',I3,/, + +' Database size=',I4,' Maximum gel reading length=',I4) + LLINO = 0 + IF(NGELS.LT.1)GO TO 5 + DO 3 I=1,NGELS + CALL READR(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) +3 CONTINUE + N=IDBSIZ-NCONTS + MXT = 0 + DO 4 I=N,IDBSIZ-1 + CALL READR(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + IF(RELPG(I).GT.MXT) THEN + MXT = RELPG(I) + LLINO = LNBR(I) + END IF +4 CONTINUE +5 CONTINUE + NAMPRO(LL+2:LL+2) = COPYNO(1:1) + IERR=0 + RETURN +100 CONTINUE + WRITE(KBOUT,9999) +9999 FORMAT(' Error encountered opening database files') + NAMPRO(LL+2:LL+2) = COPYNO(1:1) + RETURN +50 CONTINUE + WRITE(KBOUT,2000)MAXGLM +2000 FORMAT(' Error reading maximum gel reading length',/, + +' The maximum gel reading length must be a multiple', + +' of 512.',/,' E.G. 512, 1024, 1536, 2048, etc up to',I6) + MN = 512 + MX = MAXGLM + MAXIN1 = MN + CALL GETINT(MN,MX,MAXIN1, + +'Maximum gel reading length', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXGEL = IVAL + IF(MOD(MAXGEL,MAXIN1).NE.0)GO TO 50 +C PROBLEM WRITING TO RECORD IDBSIZ WITH NO SUBROUTINE CALL!!!!!!!!!! + WRITE(IDEVN,REC=IDBSIS)IDBSIZ,MAXGEL + GO TO 20 +51 CONTINUE + WRITE(KBOUT,2003) +2003 FORMAT(' Error reading protein or dna label') + CALL YESNO(ANS,'DNA', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) RETURN + IF(ANS.EQ.0)IDM = 5 + IF(ANS.EQ.1)IDM = 26 +C PROBLEM WRITING TO RECORD IDBSIZ WITH NO SUBROUTINE CALL!!!!!!!!!! + WRITE(IDEVN,REC=IDBSIS)IDBSIZ,MAXGEL,IDM + GO TO 21 + END + SUBROUTINE DBSTAR(NAMPRO,GEL,IDBSIS,IDBSIZ,KBIN,KBOUT, + +IDEV1,IDEV2,IDEV3,IERR,IHELPS,IHELPE,IDEVH,FILEH,MAXGEL, + +MAXGLM,IDM) + CHARACTER GEL(MAXGLM),FILEH*(*) + CHARACTER NAMPRO*(*) + INTEGER IWORD + PARAMETER (IWORD=4) +C WRITE(KBOUT,1005) +1005 FORMAT( + +' Define:',/, + +' 1) the maximum database size ', + +'(= number of gel readings + number of contigs)',/, + +' 2) the maximum gel reading length',/, + +' 3) whether the database is for DNA or protein sequences') + IERR=1 +3 CONTINUE + MN = 0 + CALL GTSTR('New project name',' ',NAMPRO,MN,KBOUT,KBIN,INFLAG) + IF(MN.LT.1) RETURN + LL = MIN(12,MN) + CALL CCASE(NAMPRO,1) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + GO TO 3 + END IF + MN = 10 + MX = IDBSIS + IDBSIZ = 50 + CALL GETINT(MN,MX,IDBSIZ, + +'Database size', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IDBSIZ = IVAL +5 CONTINUE + MN = 512 + MX = MAXGLM + MAXIN1 = MIN(512,MAXGEL) + CALL GETINT(MN,MX,MAXIN1, + +'Maximum gel reading length', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,FILEH,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXGEL = IVAL + IF(MOD(IVAL,MAXIN1).NE.0) THEN + MAXGEL = 512 + (IVAL/512)*512 + WRITE(KBOUT,*)'Maximum set to',MAXGEL + END IF + CALL YESNO(IDM,'Database is for DNA', + +IHELPS,IHELPE,FILEH,IDEVH,KBIN,KBOUT) + IF(IDM.LT.0) RETURN + IF(IDM.EQ.0)IDM = 5 + IF(IDM.EQ.1)IDM = 26 + NAMPRO(LL+1:LL+1)='.' + NAMPRO(LL+2:LL+2)='R' + NAMPRO(LL+3:LL+3)='L' + NAMPRO(LL+4:LL+4)='0' + CALL OPENRS(IDEV1,NAMPRO,IOK,4,3) + IF(IOK.NE.0)GO TO 100 + WRITE(KBOUT,1004) +1004 FORMAT(' Writing new database files') + J = 0 + K = 0 + N = 0 + M = 0 + DO 10 I=1,IDBSIZ + CALL WRITER(IDEV1,I,J,K,M,N) +10 CONTINUE + NAMPRO(LL+2:LL+2)='S' + NAMPRO(LL+3:LL+3)='Q' + IREC=MAXGEL/IWORD + IF(MOD(MAXGEL,IWORD).NE.0)IREC=IREC+1 + CALL OPENRS(IDEV2,NAMPRO,IOK,IREC,3) + IF(IOK.NE.0)GO TO 100 + CALL FILLC(GEL,MAXGEL,' ') + CALL WRITEW(IDEV2,IDBSIZ,GEL,MAXGEL) + NAMPRO(LL+2:LL+2)='A' + NAMPRO(LL+3:LL+3)='R' + CALL OPENRS(IDEV3,NAMPRO,IOK,3,3) + IF(IOK.NE.0)GO TO 100 +C NOTE HERE IS A WRITE TO NAMES FILE NOT USING A SUBROUTINE CALL + WRITE(IDEV3,REC=IDBSIS)IDBSIZ,MAXGEL,IDM + WRITE(KBOUT,1003)NAMPRO(1:LL),IDBSIZ +1003 FORMAT(' Database ',A,' version 0, size',I5, + +' successfully started') + WRITE(KBOUT,1006)NAMPRO(1:LL) +1006 FORMAT( + +' Note the 3 database files are named ',A,'.AR0, .SQ0, .RL0') + IERR=0 + NAMPRO(LL+2:LL+2) = '0' + RETURN +100 CONTINUE + WRITE(KBOUT,9999) +9999 FORMAT(' Error when trying to open database,', + +' database not started') + RETURN + END + SUBROUTINE ENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,GEL2,IDBSIZ,IDEV,KBIN,KBOUT,IDEVR,IDEVW,IDEVN,IDEVG, + +LINLEN,PERCD,NAMARC, + +HELPS1,HELPE1,HELPS2,HELPE2,FILEH,IDEVH,MAXGEL,IDM,NCONTC) + CHARACTER FILEH*(*) + INTEGER HELPS1,HELPS2,HELPE1,HELPE2 +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER X,Y,ANS + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),NAMARC*(*) + CHARACTER NAMARK*10 + CHARACTER GEL2(MAXGEL) +C WRITE(KBOUT,1000) +C1000 FORMAT( +C +' Enter a new gel reading into the database.',//, +C +' Note that before a gel reading is entered into the',/, +C +' database it must be compared with the database to ',/, +C +' search for overlaps using a function such as auto assemble',/, +C +' The replies to all the questions that follow are given',/, +C +' by these searches',//) + IF((IDBSIZ-(NGELS+NCONTS)).GT.2)GO TO 5 + WRITE(KBOUT,1999)IDBSIZ +1999 FORMAT(' Current database size=',I5,' extend with copy') + RETURN +5 CONTINUE +C WRITE(KBOUT,1018) +C1018 FORMAT( +C +' We need the name of the file containing the gel reading') + NAMARC = ' ' + CALL OPENF1(IDEVG,NAMARC,0,IOK,KBIN,KBOUT, + +'File name of gel reading to enter', + +HELPS1,HELPE1,FILEH,IDEVH) + IF(IOK.NE.0)RETURN + IF(NGELS.GT.0) THEN + DO 10 J=1,NGELS + CALL READN(IDEVN,J,NAMARK) + IF(NAMARC(1:10).NE.NAMARK)GO TO 10 + WRITE(KBOUT,1013)J +1013 FORMAT( ' Gel reading already in database (number,',I6, + + '), entry aborted') + RETURN +10 CONTINUE + END IF + IDIM=MAXGEL + CALL ARRFIM(IDEVG,GEL,IDIM,KBOUT) + CLOSE(UNIT=IDEVG) + NGELS=NGELS+1 + LNGTHG(NGELS)=IDIM + NAMARK=NAMARC(1:10) + CALL WRITEN(IDEVN,NGELS,NAMARK) + WRITE(KBOUT,1003)NGELS +1003 FORMAT( ' This gel reading has been given the number ',I6) + ANS=0 + IF(NGELS.GT.1)THEN +C WRITE(KBOUT,1017) +C1017 FORMAT( +C +' We need to know if the gel reading overlaps data already in', +C +' the database') + CALL YESNO(ANS,'New gel overlaps a contig', + + HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) THEN + NGELS = NGELS - 1 + RETURN + END IF + IF(ANS.EQ.0)GO TO 100 + END IF +C +C DOES NOT OVERLAP SO IT STARTS A CONTIG OF ITS OWN + LNBR(NGELS)=0 + RNBR(NGELS)=0 + RELPG(NGELS)=1 + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) + NCONTS=NCONTS+1 + N=IDBSIZ-NCONTS + LNBR(N)=NGELS + RNBR(N)=NGELS + RELPG(N)=(IDIM) + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + CALL WRITEW(IDEVW,NGELS,GEL,MAXGEL) + RETURN +C +100 CONTINUE +C WRITE(KBOUT,1019) +C1019 FORMAT( +C +' We need to know if the new gel reading overlaps in the',/, +C +' normal or complementary sense') +C COMPLEMENT GEL IF REQUIRED + CALL YESNO(ANS,'Gel overlaps in normal sense', + +HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0) THEN + NGELS = NGELS - 1 + RETURN + END IF + IF(ANS.EQ.1) THEN + CALL SQREV(GEL,IDIM) + CALL SQCOM(GEL,IDIM) + LNGTHG(NGELS)=-1*LNGTHG(NGELS) + END IF +C WRITE(KBOUT,1020) +C1020 FORMAT( +C +' We need to know which contig the new gel reading overlaps') + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON,NCONTC, + +IERR,IDBSIZ,KBIN,KBOUT,IDEVN, + +HELPS1,HELPE1,FILEH,IDEVH) + IF(IERR.NE.0) THEN + NGELS=NGELS-1 + RETURN + END IF +C WRITE(KBOUT,1014) +C1014 FORMAT( +C +' We need information about the position of the overlap.',/, +C +' First there are two types: those that extend the contig',/, +C +' leftwards and those that start internally.') + CALL YESNO(ANS,'Left end of gel reading is inside contig', + + HELPS1,HELPE1,FILEH,IDEVH,KBIN,KBOUT) + IF(ANS.LT.0)THEN + NGELS=NGELS-1 + RETURN + END IF + IF(ANS.EQ.1)GO TO 400 +C RIGHT END OR INTERNAL OVERLAP +C +C WRITE(KBOUT,1015) +C1015 FORMAT(' Now we need to know the exact position of the overlap') +160 CONTINUE + MN = 1 + MX = RELPG(LINCON) + X = 0 + CALL GETINT(MN,MX,X, + +'Position in contig of left end of gel reading', + +IVAL,KBIN,KBOUT, + +HELPS1,HELPE1,FILEH,IDEVH,IOK) + IF(IOK.NE.0) THEN + NGELS = NGELS - 1 + RETURN + END IF + X = IVAL + IF(X.EQ.0)GO TO 160 + N=NCONTC +200 CONTINUE + IF(RELPG(N).GT.X)GO TO 250 + IF(RNBR(N).EQ.0)GO TO 350 + N=RNBR(N) + GO TO 200 +250 CONTINUE +C GEL LIES BETWEEN N AND LNBR(N) +C DISPLAY JOINT + NOPT = 1 + CALL BEDIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,GEL2,LINCON,NCONTC,NOPT,X,IDBSIZ,IDEV,KBIN,KBOUT, + +IDEVR,IDEVW,IDEVN,LINLEN,PERCD, + +HELPS1,HELPE1,HELPS2,HELPE2,FILEH,IDEVH,MAXGEL,IDM) +C CHECK ON RESULT + IF(NOPT.EQ.1)GO TO 160 + IF(NOPT.EQ.3)GO TO 260 +C COCKUP + NGELS=NGELS-1 + RETURN +260 CONTINUE + CALL WRITEW(IDEVW,NGELS,GEL,MAXGEL) + LNBR(NGELS)=LNBR(N) + RNBR(NGELS)=N + RELPG(NGELS)=X + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) + RNBR(LNBR(N))=NGELS + K=LNBR(N) + CALL WRITER(IDEVR,K,RELPG(K),LNGTHG(K), + +LNBR(K),RNBR(K)) + LNBR(N)=NGELS + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + IDIM=ABS(LNGTHG(NGELS)) + Y=X+(IDIM)-1 + IF(Y.LE.RELPG(LINCON))RETURN + RELPG(LINCON)=Y + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) + RETURN +350 CONTINUE +C MUST BE A RIGHT END OVERLAP +C DSPLAY JOINT + NOPT = 1 + CALL BEDIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,GEL2,LINCON,NCONTC,NOPT,X,IDBSIZ,IDEV,KBIN,KBOUT, + +IDEVR,IDEVW,IDEVN,LINLEN,PERCD, + +HELPS1,HELPE1,HELPS2,HELPE2,FILEH,IDEVH,MAXGEL,IDM) + IF(NOPT.EQ.1)GO TO 160 + IF(NOPT.EQ.3)GO TO 360 +C COCKUP + NGELS=NGELS-1 + RETURN +360 CONTINUE + CALL WRITEW(IDEVW,NGELS,GEL,MAXGEL) + LNBR(NGELS)=N + RNBR(NGELS)=0 + RELPG(NGELS)=X + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) + RNBR(N)=NGELS + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) + RNBR(LINCON)=NGELS + IDIM=ABS(LNGTHG(NGELS)) + Y=X+(IDIM)-1 + RELPG(LINCON)=MAX(RELPG(LINCON),Y) + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + RETURN +C +400 CONTINUE +C +C ADDING TO LEFT END +C WRITE(KBOUT,1015) +410 CONTINUE + MN = 1 + MX = IDIM + X = 0 + CALL GETINT(MN,MX,X, + +'Position in new gel reading of left end of contig', + +IVAL,KBIN,KBOUT, + +HELPS1,HELPE1,FILEH,IDEVH,IOK) + IF(IOK.NE.0) THEN + NGELS = NGELS - 1 + RETURN + END IF + X = IVAL + IF(X.EQ.0)GO TO 410 +C SET FLAG TO SHOW LEFT END OVERLAP + NOPT=2 + CALL BEDIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,GEL2,LINCON,NCONTC,NOPT,X,IDBSIZ,IDEV,KBIN,KBOUT, + +IDEVR,IDEVW,IDEVN,LINLEN,PERCD, + +HELPS1,HELPE1,HELPS2,HELPE2,FILEH,IDEVH,MAXGEL,IDM) + IF(NOPT.EQ.1)GO TO 400 + IF(NOPT.EQ.3)GO TO 420 +C COCKUP + NGELS=NGELS-1 + RETURN +420 CONTINUE + CALL WRITEW(IDEVW,NGELS,GEL,MAXGEL) + RELPG(NGELS)=1 + RNBR(NGELS)=NCONTC + LNBR(NGELS)=0 + CALL WRITER(IDEVR,NGELS,RELPG(NGELS),LNGTHG(NGELS), + +LNBR(NGELS),RNBR(NGELS)) + LNBR(NCONTC)=NGELS + RELPG(NCONTC)=X + CALL WRITER(IDEVR,NCONTC,RELPG(NCONTC),LNGTHG(NCONTC), + +LNBR(NCONTC),RNBR(NCONTC)) + RELPG(LINCON)=RELPG(LINCON)+X-1 + IDIM=ABS(LNGTHG(NGELS)) + Y=IDIM + IF(Y.GT.RELPG(LINCON))RELPG(LINCON)=Y + LNBR(LINCON)=NGELS + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + N=NCONTC +440 CONTINUE + IF(RNBR(N).EQ.0)RETURN + N=RNBR(N) + RELPG(N)=RELPG(N)+X-1 + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) + GO TO 440 + END + SUBROUTINE PADCON(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +GEL,LINCON,POSN,NC,IDBSIZ,IDEVR,IDEVW,MAXGEL,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),POSN,X + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL) + CHARACTER PAD + SAVE PAD + DATA PAD/'*'/ +C NOW FIND FIRST CHAR THAT OVERLAPS REGION + LLINO=LNBR(LINCON) +30 CONTINUE + X=RELPG(LLINO)+ABS(LNGTHG(LLINO))-1 + IF(X.GE.POSN)GO TO 40 +C NOT IN REGION + LLINO=RNBR(LLINO) + GO TO 30 +40 CONTINUE +C NOW GET THIS GEL FROM DISK +C READ(IDEVW,REC=LLINO)GEL + CALL READW(IDEVW,LLINO,GEL,MAXGEL) +C CALC POSN IN THIS GEL TO EDIT + X=POSN-RELPG(LLINO)+1 + K=X +C MOVE THE DATA RIGHT + M=ABS(LNGTHG(LLINO)) + LNGTHG(LLINO)=LNGTHG(LLINO)+SIGN(NC,LNGTHG(LLINO)) +C CHECK FOR OVER END OF ARRAY + N=ABS(LNGTHG(LLINO)) + IF(N.GT.MAXGEL)THEN + WRITE(KBOUT,1000)LLINO +1000 FORMAT( + +' Data pushed off end of gel',I4,' during padding') + M=M-(N-MAXGEL) + N=MAXGEL + LNGTHG(LLINO)=SIGN(MAXGEL,LNGTHG(LLINO)) + END IF + J=M-K+1 + DO 55 I=1,J + GEL(N)=GEL(M) + N=N-1 + M=M-1 +55 CONTINUE +C PERFORM THE INSERTION + DO 60 I=K,MIN(MAXGEL,K+NC-1) + GEL(I)=PAD +60 CONTINUE +C WRITE BACK TO DISK + CALL WRITEW(IDEVW,LLINO,GEL,MAXGEL) +C WRITE(IDEVW,REC=LLINO)GEL +C WRITE NEW LINE + CALL WRITER(IDEVR,LLINO,RELPG(LLINO),LNGTHG(LLINO), + +LNBR(LLINO),RNBR(LLINO)) +C WRITE(IDEVR,REC=LLINO) +C 1RELPG(LLINO),LNGTHG(LLINO),LNBR(LLINO),RNBR(LLINO) +65 CONTINUE +C NOW GET NEXT GEL + LLINO=RNBR(LLINO) +C LAST GEL? + IF(LLINO.EQ.0)GO TO 70 +C DOES IT HAVE DATA IN REGION? +C IE DO RELPG AND RELPG+LNGTHG-1 LIE EITHER SIDE OF POSN? + IF(RELPG(LLINO).GT.POSN)GO TO 70 + X=RELPG(LLINO)+ABS(LNGTHG(LLINO))-1 + IF(X.LT.POSN)GO TO 65 +C WITHIN + GO TO 40 +70 CONTINUE +C INSERTS FINISHED SO NEED TO INCREMENT ALL THOSE GELS TO RIGHT + LLINO=LNBR(LINCON) +75 CONTINUE + IF(RELPG(LLINO).GT.POSN)GO TO 80 +76 CONTINUE + LLINO=RNBR(LLINO) + IF(LLINO.EQ.0)GO TO 90 + GO TO 75 +80 CONTINUE + RELPG(LLINO)=RELPG(LLINO)+NC +C WRITE NEW LINE + CALL WRITER(IDEVR,LLINO,RELPG(LLINO),LNGTHG(LLINO), + +LNBR(LLINO),RNBR(LLINO)) +C WRITE(IDEVR,REC=LLINO) +C 1RELPG(LLINO),LNGTHG(LLINO),LNBR(LLINO),RNBR(LLINO) + GO TO 76 +90 CONTINUE +C NEED TO INCREMENT CONTIG LINE + RELPG(LINCON)=RELPG(LINCON)+NC + CALL WRITER(IDEVR,LINCON,RELPG(LINCON),LNGTHG(LINCON), + +LNBR(LINCON),RNBR(LINCON)) +C WRITE(IDEVR,REC=LINCON)RELPG(LINCON),LNGTHG(LINCON),LNBR(LINCON), +C 1RNBR(LINCON) + RETURN + END + SUBROUTINE AUTOJ(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ, + +NGELS,NCONTS,MAXGEL, + +TEMP3,WORDP,WORDN,LPOWRC,POSNS,GELN, + +SEQ1,MAXSEQ,SEQ2,SEQ3,SEQ4,SEQ5,SEQC2,SEQG2,MATCH, + +MAXGLM,MAXGL2,CHRSIZ,ECHRSZ,LENGTH, + +SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON,CONST, + +KBIN,KBOUT,IDEV1,IDEV2,IDEV3,IDEV4,IDEV7,IDEV8,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH,NAMARC,NAMPRO,FILE, + +PERCD,IOPEN,IDM,SEQG3,SEQC3,IOK) + INTEGER CHRSIZ,ECHRSZ + INTEGER RELPG(MAXDB) + INTEGER LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + INTEGER JOINT(2),ITOTPC(2),ITOTPG(2),IDIM22(2),IDOUT(2) + INTEGER LLINO(2),ITYPE(2),IFAIL(2) + INTEGER ILEFTS(2),ILC(2),IPOSC(2),IPOSG(2),ISENSE(2) + INTEGER ANS,WINDOW + INTEGER TEMP3(ECHRSZ,MAXGL2),CONST(LENGTH) + INTEGER POSNS(MAXSEQ),WORDP(LPOWRC),WORDN(LPOWRC),GELN(MAXGLM) + INTEGER CENDS(MAXCON),NENDS(MAXCON) + CHARACTER SEQ3(MAXGLM),SEQC2(MAXGLM,2),SEQG2(MAXGLM,2) + CHARACTER SEQ1(MAXSEQ),SEQ2(MAXGLM),MATCH(MAXGLM),SEQ4(MAXGLM) + INTEGER SAV1(MAXSAV),SAV2(MAXSAV),SAV3(MAXSAV) + CHARACTER NAMARC*(*),NAMPRO*(*),FILE*(*) + CHARACTER SEQ5(MAXGLM),HELPF*(*),SEQG3(MAXGLM),SEQC3(MAXGLM) + CALL DBCHEK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + TEMP3,IERR,KBOUT) + IF(IERR.GT.1) RETURN + IFAIL(1) = 0 + IF(NGELS.LT.1) RETURN + MN = LENGTH*2 + MX = MAXGLM + 1 + MINMAT = MAX(15,MN) + CALL GETINT(MN,MX,MINMAT, + +'Minimum initial match', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINMAT = IVAL + MN = 2 + MX = 5 + MINSLI = MAX(3,MN) + CALL GETINT(MN,MX,MINSLI, + +'Minimum alignment block', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINSLI = IVAL + MN = 0 + MX = 25 + MAXPG = 8 + CALL GETINT(MN,MX,MAXPG, + +'Maximum pads per sequence', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXPG = IVAL + MAXPC = IVAL + RMN = 0. + RMX = 20. + PERMAX = 8. + CALL GETRL(RMN,RMX,PERMAX, + + 'Maximum percent mismatch after alignment', + + VAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + PERMAX = VAL + MN = MINMAT + MX = MAXGEL + WINDOW = 100 + CALL GETINT(MN,MX,WINDOW, + +'Probe length', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + WINDOW = IVAL + IWING = 0 + IDIM1=0 + MAXOVR=MAXGEL-3*MAX(MAXPC,MAXPG) + ANS=0 + CALL JCONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,MAXSEQ,SEQ2,IDBSIZ,IDIM1,ANS,KDUMM,KDUMM,KDUMM,TEMP3, + +ECHRSZ,MAXGL2,KBOUT,IDEV2,IFAIL(1),MAXGEL,IDM,PERCD,SEQ5, + +CENDS,NENDS,MAXCON,IWING,NBAD) + IDIM2=MAXGEL +C +C Note I am doing something horrible here to save space: +C sending cends and nends to jcons to get the extension lengths +C then swapping them with temp3 by sending temp to ajoin and +C receiving them as cends and nends, and sending cends nends +C and receiving them as iladd,iradd + CALL AUTOJN(SEQ1,IDIM1,SEQ2,IDIM2,ILEFTS,ILC,IPOSC, + +IPOSG,ISENSE,LLINO,IMATC,IFCOMP,MINMAT,POSNS,WORDP,WORDN, + +CONST,LENGTH,LPOWRC,IDEV,MATCH,MAXGEL,MAXGLM,SEQ5,GELN, + +SAV1,SAV2,SAV3,MAXSAV,TEMP3,TEMP3(MAXCON+1,1),MAXCON, + +SEQG2,SEQC2,SEQ4,IDOUT,IDIM22,ITOTPG,ITOTPC,JOINT,IFAIL, + +ITYPE,MAXPC,MAXPG,PERMAX,MINSLI,SEQG3,SEQC3,KFAIL, + +WINDOW,CENDS,NENDS,RELPG,LNBR,IDBSIZ,NCONTS) + END + SUBROUTINE AUTOJN(SEQ1,IDIMIN,GEL,IDIMGI,ILEFTS,ILC,IPOSC, + +IPOSG,ISENSE,LLINO,IMATC,IFCOMP,MINMAT,POSNS,WORDP,WORDN, + +CONST,LENGTH,LPOWRC,KBOUT,MATCH,MAXGEL,MAXGLM,GELCOP,GELN, + +SAVPS,SAVPG,SAVL,MAXSAV,CENDS,NENDS,MAXCON, + +SEQG2,SEQC2,SEQ4,IDOUT,IDIM22,ITOTPG,ITOTPC,JOINT,IFAIL, + +ITYPE,MAXPC,MAXPG,PERMAX,MINSLI,SEQG3,SEQC3,KFAIL, + +WINDOW,ILADD,IRADD,RELPG,LNBR,IDBSIZ,NCONTS) +C AUTHOR: RODGER STADEN + INTEGER ILEFTS(2),ILC(2),IPOSC(2),IPOSG(2),ISENSE(2),LLINO(2) + INTEGER POSNS(IDIMIN),GELN(MAXGLM),WORDP(LPOWRC),SAVPS(MAXSAV) + INTEGER SAVPG(MAXSAV),SAVL(MAXSAV) + INTEGER WORDN(LPOWRC) + CHARACTER GELCOP(MAXGLM),MATCH(MAXGLM) + INTEGER CENDS(MAXCON),NENDS(MAXCON),ILADD(MAXCON),IRADD(MAXCON) + INTEGER CONST(LENGTH) + CHARACTER SEQ1(IDIMIN),GEL(MAXGLM) + CHARACTER SEQG2(MAXGLM,2),SEQC2(MAXGLM,2),SEQ4(MAXGLM) + INTEGER IDOUT(2),IDIM22(2),ITOTPG(2),ITOTPC(2),JOINT(2) + INTEGER IFAIL(2),ITYPE(2) + PARAMETER (MAXC = 10) + CHARACTER SEQG3(MAXGLM),SEQC3(MAXGLM) + INTEGER JLEFTS(MAXC),JLC(MAXC),JPOSC(MAXC),JPOSG(MAXC),MCON(MAXC) + INTEGER JSENSE(MAXC),JLLINO(MAXC),WINDOW + INTEGER RELPG(IDBSIZ),LNBR(IDBSIZ) + IFAIL(1) = 1 + IFAIL(2) = 1 + KFAIL = 0 + IDCEND=MAXCON + CALL FNDCON(SEQ1,IDIMIN,CENDS,NENDS,IDCEND,MAXCON,KBOUT) +C find possible missed joins +C we have consensus in seq1 in order first contig,second contig etc +C compare the ends in reverse order, simultaneously shortening the consensus + IDIM = IDIMIN + DO 200 JCON = IDCEND,2,-1 + IDIM = CENDS(JCON) - 1 + CALL ENCO(SEQ1,IDIM,POSNS,CONST,LENGTH) + CALL ENCONA(POSNS,IDIM,WORDP,WORDN,LPOWRC,LENGTH) + JS = CENDS(JCON) + 20 + JE = CENDS(JCON+1) - 1 + IEND = 1 + IDIMG = MIN(WINDOW,JE-JS+1) +C check for case where contig is shorter than probe (window) +C in which case only compare the left hand end + IF(JE-JS+1.LE.WINDOW) IEND = 2 +C WRITE(*,*)JCON,JS,JE,IDIMG +1 CONTINUE + IMATC = 0 + IF(IEND.EQ.1) THEN + CALL SQCOPY(SEQ1(JE-IDIMG+1),GEL,IDIMG) + ELSE + CALL SQCOPY(SEQ1(JS),GEL,IDIMG) + END IF + CALL SQCOPY(GEL,GELCOP,IDIMG) + ISTRAN=1 +2 CONTINUE + CALL ENCO(GEL,IDIMG,GELN,CONST,LENGTH) + IDSAV=MAXSAV + CALL CFGEL(GELN,IDIMG,POSNS,IDIM,WORDP,WORDN,LENGTH,LPOWRC, + +SAVPG,SAVPS,SAVL, + +IDSAV,SEQ1,GEL,MINMAT,IFCOMP,KBOUT) + IF(IFCOMP.NE.0) THEN + CALL ERROM(KBOUT,'Error in CFGEL') + RETURN + END IF + IF(IDSAV.NE.0)THEN + CALL ADISM5(IDIM,IDIMG,SAVPS,SAVPG,IDSAV,CENDS,NENDS, + + IDCEND,MAXCON,JLEFTS,JLC,JPOSC,JPOSG,JSENSE,JLLINO, + + IMATC,ISTRAN,MAXC,KBOUT,MCON) + END IF + ISTRAN=ISTRAN+1 + IF(ISTRAN.EQ.2) THEN + CALL SQCOPY(GELCOP,GEL,IDIMG) + CALL SQREV(GEL,IDIMG) + CALL SQCOM(GEL,IDIMG) + GO TO 2 + END IF + CALL SQCOPY(GELCOP,GEL,IDIMG) + KSENSE = 0 +C WRITE(KBOUT,*)'Total matches found',IMATC + IF(IMATC.NE.0) THEN + JMATC = 0 + DO 100 I = 1,IMATC + IF(JSENSE(I).EQ.-1) THEN + IF(KSENSE.EQ.0) THEN + CALL SQREV(GEL,IDIMG) + CALL SQCOM(GEL,IDIMG) + KSENSE = 1 + END IF + END IF + JDIM22 = IDIMG + JDOUT = MAXGEL + IDSAV = MAXSAV +C IF(IEND.EQ.1) WRITE(KBOUT,*)'Left end of Contig',NENDS(JCON) +C IF(IEND.EQ.2) WRITE(KBOUT,*)'Right end of Contig',NENDS(JCON) +C WRITE(KBOUT,*)'Trying to align with contig',JLLINO(I) + CALL ALINEJ(SEQ1(JLEFTS(I)),GEL,SEQG3,SEQC3, + + SAVPS,SAVPG,SAVL,IDSAV,JLC(I),JDIM22,JDOUT, + + JPOSC(I),JPOSG(I),MINSLI,JJOINT,JTOTPC,JTOTPG, + + JFAIL,JTYPE,MAXPC,MAXPG,PERMAX,KBOUT,SEQ4,MAXGEL,PERMS, + + NENDS(JCON),IEND,JLLINO(I),JSENSE(I),ILADD,IRADD,MAXCON,MCON(I), + + JCON,RELPG,LNBR,IDBSIZ,NCONTS,WINDOW) +100 CONTINUE + END IF + IEND = IEND + 1 + IF(IEND.EQ.2) GO TO 1 +C IDIM = CENDS(JCON)-1 + 200 CONTINUE + END + SUBROUTINE ALINEJ(SEQ1,SEQ2,SEQG2,SEQC2,ISAV1,ISAV2,ISAV3, + +IDSAV,IDC,IDIM2,IDOUT,IC1,IG1,MINSLI,JOINT, + +ITOTPC,ITOTPG,IFAIL,ITYPE,MAXPC,MAXPG,PERMAX,KBOUT,SEQ3,MAXGEL, + +PERCM,JCONN,IEND,NCON,JSENSE,ILADD,IRADD,MAXCON,MCON,JCON, + +RELPG,LNBR,IDBSIZ,NCONTS,WINDOW) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDC),SEQ2(IDIM2),SEQG2(IDOUT),SEQC2(IDOUT) + CHARACTER SEQ3(MAXGEL) + INTEGER ISAV1(IDSAV),ISAV2(IDSAV),ISAV3(IDSAV),WINDOW + INTEGER ILADD(MAXCON),IRADD(MAXCON),RELPG(IDBSIZ),LNBR(IDBSIZ) + MINSLT=MINSLI +C SAVE SEQ2 + CALL SQCOPY(SEQ2,SEQ3,IDIM2) + CALL MSTLKL(SEQ3,IDIM2) + IFAIL=1 +C FIND MATCHES + IPP=IDSAV + CALL SLIDER(SEQ1,IDC,SEQ3,IDIM2,IC1,IG1,MAXPG,MAXPC,MINSLT, + +ISAV1,ISAV2,ISAV3,IPP) + IF(IPP.GT.IDSAV)RETURN + IF(IPP.LT.1)RETURN + CALL REMOVL(ISAV2,ISAV3,ISAV1,IPP) + CALL BUB3AS(ISAV2,ISAV3,ISAV1,IPP) +C DO TOPOLOGICAL CHECK + CALL TPCHEK(ISAV2,ISAV3,ISAV1,IPP) + IFAIL=0 + CALL LINEUP(SEQ2,SEQ1,SEQG2,SEQC2,IDC,IDIM2,IDOUT,ISAV3,ISAV2, + +ISAV1,IPP,ITOTPC,ITOTPG,JOINT,ITYPE,KBOUT,MAXGEL,IFAIL) + IF(IFAIL.NE.0)RETURN +C IDIM2 IS NOW LENGTH OF ALIGNED GEL +C WRITE(KBOUT,1052)ITOTPC,ITOTPG +C1052 FORMAT(' Padding in contig= ',I4,' and in gel= ',I4) + CALL JALIGN(SEQC2,SEQG2,SEQ3,MAXGEL,IDOUT,IDIM2,JOINT, + +ITYPE,PERCM,KBOUT,IFAIL,PERMAX,JCONN,IEND,NCON,JSENSE, + +ILADD,IRADD,MAXCON,MCON,JCON,RELPG,LNBR,IDBSIZ,NCONTS,WINDOW) +C ARE ALL CHECKS OK? + IF(ITOTPC.GT.MAXPC)IFAIL=1 + IF(ITOTPG.GT.MAXPG)IFAIL=1 + IF(PERCM.GT.PERMAX)IFAIL=1 + END +C SUBROUTINE DALIGN +C +C COUNTS MISMATCHES AND DISPLAYS OVERLAP. + SUBROUTINE JALIGN(SEQC2,SEQG2,SEQ3,MAXGEL,IDOUT,IDIM2, + +JOINT,ITYPE,X,KBOUT,IFAIL,PERMAX,JCONN,IEND,NCON,JSENSE, + +ILADD,IRADD,MAXCON,MCON,JCON,RELPG,LNBR,IDBSIZ,NCONTS,WINDOW) +C AUTHOR: RODGER STADEN + CHARACTER SEQC2(MAXGEL),SEQG2(MAXGEL),SEQ3(MAXGEL) + CHARACTER PAD,DASH,STRAND,NAME1*6,NAME2*6 + INTEGER ILADD(MAXCON),IRADD(MAXCON),RELPG(IDBSIZ),LNBR(IDBSIZ) + INTEGER CLINNO,WINDOW + EXTERNAL CLINNO + SAVE PAD,DASH + DATA PAD,DASH/',','-'/ + IENDG=1 + IENDC=JOINT +C ONLY LOOK AT OVERLAP WHICH IS FROM JOINT FOR LEFT TYPE JOIN + IF(ITYPE.EQ.1)THEN + IENDG=JOINT + IENDC=1 + END IF +100 CONTINUE +C LENGTH OF OVERLAP? + LG=IDIM2-IENDG+1 + LO=MIN(IDOUT,LG) +C SAVE RAW DATA + CALL SQCOPY(SEQG2,SEQ3,IDIM2) + CALL MSTLKL(SEQ3,IDIM2) + X=FLOAT(LO) + Y=X + K=IENDG+LO-1 +C POINT TO CONSENSUS + J=0 +C CHECK FOR OVERFLOW + IF(K.GT.MAXGEL)THEN + WRITE(KBOUT,1001) +1001 FORMAT( + +' Matching region too long for routine dalign. Alignment aborted') + IFAIL=1 + RETURN + END IF + DO 200 I=IENDG,K + J=J+1 + IF(SEQC2(J).EQ.SEQ3(I))GO TO 200 +C IF(SEQ3(I).EQ.DASH)GO TO 200 +C IF(SEQC2(J).EQ.DASH)GO TO 200 +C IF(SEQC2(J).EQ.PAD)GO TO 200 + X=X-1. +200 CONTINUE + X=(Y-X)*100./Y + IF(X.LT.PERMAX) THEN + IC = CLINNO(LNBR,IDBSIZ,NCONTS,JCONN) + IF(IC.EQ.0) THEN + IFAIL = 99 + RETURN + END IF + IF(JSENSE.EQ.-1) THEN + STRAND = '-' + IF(IEND.EQ.1) THEN +C +C probe is complement of right hand end of contig. Give posns assuming +C this contig is complemented. +C + IENDGT = IENDG - IRADD(JCON) +C +C Next line gives posns relative to original orientation +C IENDGT = RELPG(IC) + IRADD(JCON) - IENDG + 1 + ELSE +C +C probe is complement of left end of contig. Give posns assuming this +C contig is going to be complemented. +C + IENDGT = RELPG(IC) - WINDOW + ILADD(JCON) + IENDG + END IF + ELSE + STRAND = '+' + IF(IEND.EQ.2) THEN +C +C probe is left hand end of contig in original sense +C + IENDGT = IENDG - ILADD(JCON) + ELSE +C +C probe is right hand end of contig in original sense +C + IENDGT = RELPG(IC) + IRADD(JCON) - WINDOW + IENDG + END IF + END IF + IENDCT = IENDC - ILADD(MCON) + WRITE(KBOUT,*) + + ' Possible join between contig ',JCONN,' in the ', + + STRAND,' sense and contig ',NCON + WRITE(KBOUT,1000)X +1000 FORMAT(' Percentage mismatch after alignment = ',F4.1) + WRITE(NAME1,1002)JCONN + WRITE(NAME2,1002)NCON + 1002 FORMAT(I6) + CALL FMT4LP(SEQC2(1),SEQG2(IENDG),LO,IENDCT,IENDGT,KBOUT, + + NAME2,NAME1) + END IF + IFAIL=0 + END + SUBROUTINE ADISM5(IDIM,IDIMG,SAVPS,SAVPG,IDSAV, + +CENDS,NENDS,IDCEND,MAXCON,ILEFTS,ILC,IPOSC,IPOSG,ISENSE, + +LLINO,IMATC,ISTRAN,MAXC,KBOUT,MCON) +C AUTHOR: RODGER STADEN + INTEGER ILEFTS(MAXC),ILC(MAXC),IPOSC(MAXC),IPOSG(MAXC) + INTEGER ISENSE(MAXC),LLINO(MAXC),MCON(MAXC) + INTEGER CENDS(MAXCON) + INTEGER NENDS(MAXCON) + INTEGER SAVPS(IDSAV),SAVPG(IDSAV) + NEXTC=IDIM+1 + CALL BUB2AS(SAVPS,SAVPG,IDSAV) + IMATC=IMATC+1 + CALL ADISM6(SAVPS(1),SAVPG(1),CENDS,NENDS,IDCEND,MAXCON, + + ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN,NEXTC,MAXC, + + KBOUT,MCON) + LEND=IDIMG-SAVPG(1)+SAVPS(1) + DO 10 I=2,IDSAV + IF((SAVPS(I).LT.LEND).AND.(SAVPS(I).LT.NEXTC))GO TO 10 + IMATC=IMATC+1 + CALL ADISM6(SAVPS(I),SAVPG(I),CENDS,NENDS,IDCEND,MAXCON, + + ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN,NEXTC,MAXC, + + KBOUT,MCON) + LEND=IDIMG-SAVPG(I)+SAVPS(I) +10 CONTINUE + IMATC = MIN(IMATC,MAXC) + END + SUBROUTINE ADISM6(ISAVPS,SAVPG,CENDS,NENDS, + +IDCEND,MAXCON,ILEFTS,ILC,IPOSC,IPOSG,ISENSE,LLINO,IMATC,ISTRAN, + +NEXTC,MAXC,KBOUT,MCON) +C AUTHOR: RODGER STADEN + INTEGER ILEFTS(MAXC),ILC(MAXC),IPOSC(MAXC),IPOSG(MAXC) + INTEGER ISENSE(MAXC),LLINO(MAXC),MCON(MAXC) + INTEGER SAVPS,SAVPG,CENDS(MAXCON) + INTEGER NENDS(MAXCON) + SAVPS=ISAVPS-19 + JJ=1 + DO 5 J=2,IDCEND + IF(SAVPS.GT.CENDS(J))GO TO 5 + JJ=J-1 + GO TO 6 +5 CONTINUE + JJ=IDCEND +6 CONTINUE +C new bit need to save contig number for alinej + SAVPS=SAVPS-1 + LCL=SAVPS-CENDS(JJ) + LCR=CENDS(JJ+1)-ISAVPS-1 + NEXTC=CENDS(JJ+1)+20 + IF(IMATC.LE.MAXC) THEN + MCON(IMATC) = JJ + ILEFTS(IMATC)=CENDS(JJ)+20 + ILC(IMATC)=LCL+LCR+1 + IPOSC(IMATC)=LCL+1 + IPOSG(IMATC)=SAVPG + LLINO(IMATC)=NENDS(JJ) + ISENSE(IMATC)=1 + IF(ISTRAN.EQ.2)ISENSE(IMATC)=-1 + ELSE + CALL ERROM(KBOUT,'Warning: too many overlaps') + END IF + END +C JCONS + SUBROUTINE JCONS(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,IDBSIZ,ISTART,ANS,LLINO,LREG,RREG,TEMP, + +CHRSIZ,MAXGL2,KBOUT, + +IDEVW,IFAIL,MAXGEL,IDM,PERCD,TGEL,ILADD,IRADD,MAXCON, + +IWIN,NBAD) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),ANS,CHRSIZ,ILADD(MAXCON),IRADD(MAXCON) + INTEGER LREG,RREG,X,Y,TEMP(CHRSIZ,MAXGL2) + CHARACTER SEQ1(IDIM1) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER GEL(MAXGEL),TGEL(MAXGEL) + CHARACTER NAMPRO*(*) + CALL BUSY(KBOUT) + IF(IWIN.GT.0) PERC = REAL(NBAD)/REAL(IWIN) + IEND = 1 + IFAIL=0 + N=IDBSIZ-NCONTS + NCONS = 0 + DO 110 I=N,IDBSIZ-1 + J=LNBR(I) + X=1 + Y=RELPG(I) + ISTART=ISTART+1 + IF((ISTART+19+Y+2*MAXGEL).GT.IDIM1)THEN + WRITE(KBOUT,1009)IDIM1 +1009 FORMAT( + + ' Maximum consensus length(',I6,') exceeded',/, + + ' calculation aborted') + IFAIL=1 + RETURN + END IF + CALL ADDTIT(SEQ1(ISTART),NAMPRO,J,ISTART) + NCONS = NCONS + 1 + IDIN = 0 + ILADD(NCONS) = IDIN + ISTART = ISTART + IDIN + CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + SEQ1(ISTART),Y,GEL,X,Y,J,IDBSIZ,TEMP,CHRSIZ,MAXGL2,IDEVW,MAXGEL, + + IDM,PERCD) + ISTART=ISTART+Y-1 + IDIN = 0 + IRADD(NCONS) = IDIN + ISTART = ISTART + IDIN +110 CONTINUE + END diff --git a/src/staden/dialogues.c b/src/staden/dialogues.c new file mode 100644 index 0000000..7ce5bed --- /dev/null +++ b/src/staden/dialogues.c @@ -0,0 +1,985 @@ +/* + Title: dialogues + + File: dialogues.c + Purpose: Routines to provide dialogue interaction + Last update: Wed Feb 04 1991 + + Change log: + 23/01/91 SD CRAction now operative for yesnoWid + 04/02/91 SD getint_x getrl_x getrls_x fixed bug in main while loop + +*/ + + +/* + This module provides a series of routines to enable dialogue + interaction with a user. They replace some routines removed from + subs89. + + This module is initialised by a call to CreateDialogueShellWidget. + This creates the top half of the dialogue shell and a number of + ``template'' widgets which perform particular visual dialogue + patterns. At run time, when the Fortran calls come in, the + appropriate template is filled in and appended to the bottom half + of the dialogue shell. + + After each client-called dialogue has been completed, we + call dispatchCurrentEvents() to make sure everything X-wise + is up to date before we return to the FORTRAN. + + The dialogue policy, coded in `doDialogue' is to leave the current + dialogue up until a new one throws it out. The `busy_x' function + can be called just like any other to insert the busy dialogue. + + +---- + + Error messages shouldn't go to stderr, but to the text output + Ensure each switch branch sets the result code. + The coding of openf1 is fetid. + The guessing of a width for the box in radio_x is horrid. An + application resource should be set up and retrieved. +*/ + + + + +/* ---- Includes ---- */ + +#include "fort.h" /* IMPORT: int_f definition */ +#include "dialogues.h" +#include "userface.h" +#include "help.h" /* IMPORT: help */ +#include "main.h" /* IMPORT: dispatchEventsUntil, finalx */ +#include "FtoC.h" /* IMPORT: Cstr2Fstr, Fstr2Cstr */ +#include "progSpec.h" /* IMPORT: CreateProgMenus */ +#include "textOutput.h" /* IMPORT: UpdateTextOutput */ +#include "mystdlib.h" /* IMPORT: strtod, strtol */ +#include "locks.h" +#include "helpnmenu.h" + +#include +#include /* IMPORT: fopen, fgets, fseek, fprintf, sprintf */ +#include +/*#include */ /* IMPORT: strlen */ +#include "values.h" /* IMPORT: MAXLONG */ + +#include +#include +#include +#include +#include +#include +#include +#include +#include + + + + + +/* ---- Types ---- */ + + +typedef enum +{ DialogueOK, + DialogueCancel, + DialogueYES, + DialogueNO, + DialogueDefault +} DialogueResult; + + +/* ---- Global variables ---- */ + +Dimension dialogue_width; +Dimension dialogue_height; + +/* ---- Static variables ---- */ + +/* The overall dialogue form */ +static Widget dialogueFormWid; + +/* "Current function: ..." label stuff */ +static long currFunc = 0; +static Widget currFuncWid; + +/* "Current file: ..." label stuff */ +static Widget currFileWid; + + + + +/* ---- Some internal routines ---- */ + + +/* Dialogue management */ + +static Boolean dialogueDone = False; +static DialogueResult dialogueResult; +static Boolean DialogueDone() +{ return(dialogueDone); +} + +static Widget currentDialogueWid = NULL; + +static DialogueResult doDialogue(Widget dialogueWid) +{ Widget prevDialogueWid = currentDialogueWid; + + /* Swap out the previous widget, if any, put in the new one */ + currentDialogueWid = dialogueWid; + XawFormDoLayout(dialogueFormWid, False); + if (prevDialogueWid != (Widget)NULL) XtUnmanageChild(prevDialogueWid); + XtManageChild(currentDialogueWid); + XawFormDoLayout(dialogueFormWid, True); + + /* Do the dialogue */ + dialogueDone = False; + dispatchEventsUntil(DialogueDone); + + return(dialogueResult); +} + + + + +/* ---- Callback routines ---- */ + + +static void OKCallback(Widget w, + XtPointer client_data, XtPointer call_data) +{ dialogueResult = DialogueOK; + dialogueDone = True; +} + + +static void CancelCallback(Widget w, + XtPointer client_data, XtPointer call_data) +{ dialogueResult = DialogueCancel; + dialogueDone = True; +} + + +static void QuitAppCallback(Widget w, + XtPointer client_data, XtPointer call_data) +{ + if (semaphoreFree(activeLock)) + finalx(0); +} + + +static void HelpCallback(Widget w, + XtPointer client_data, XtPointer call_data) +{ help(currFunc); +} + +static void YESCallback(Widget w, + XtPointer client_data, XtPointer call_data) +{ dialogueResult = DialogueYES; + dialogueDone = True; +} + + +static void NOCallback(Widget w, + XtPointer client_data, XtPointer call_data) +{ dialogueResult = DialogueNO; + dialogueDone = True; +} + + +/* Null dialogue */ + +static Widget nullDialogueWid; + +static void CreateNullDialogue(Widget parentWid, Widget fromVertWid) +{ Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + nullDialogueWid = XtCreateManagedWidget("null", labelWidgetClass, + parentWid, + args, nargs); +} + + +/* NChoice dialogue */ + +static Widget nChoiceWid; +static Widget nChoiceButtonsWid; + +static void CreateNChoiceDialogue(Widget parentWid, Widget fromVertWid) +{ Widget okw, cw; + Arg args[10]; + int nargs; + + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + nChoiceWid = XtCreateWidget("nChoice", formWidgetClass, parentWid, + args, nargs); + + nargs = 0; + nChoiceButtonsWid = XtCreateManagedWidget("buttons", boxWidgetClass, + nChoiceWid, args, nargs); + + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, nChoiceButtonsWid); nargs++; + okw = XtCreateManagedWidget("OK", commandWidgetClass, + nChoiceWid, args, nargs); + XtAddCallback(okw, XtNcallback, OKCallback, NULL); + + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, nChoiceButtonsWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, okw); nargs++; + cw = XtCreateManagedWidget("Cancel", commandWidgetClass, + nChoiceWid, args, nargs); + XtAddCallback(cw, XtNcallback, CancelCallback, NULL); +} + + +/* getopt dialogue */ + +static Widget getoptWid; +static long getoptDialogueRes; +static Boolean getoptExec; /* True=>execute. False=>execute with dialogue */ + +static void MenuItemCallback(Widget w, + XtPointer client_data, XtPointer call_data) +/* + the number of the function selected comes as `call_data'. +*/ +{ dialogueDone = True; + getoptDialogueRes = (long) call_data; +} + +static void getoptExecCallback(Widget w, + XtPointer client_data, XtPointer call_data) +{ Arg args[1]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNlabel, + getoptExec ? "Execute with dialogue" : "Execute"); nargs++; + XtSetValues(w, args, nargs); + getoptExec = !getoptExec; +} + +static void CreateGetoptDialogue(Widget parentWid, Widget fromVertWid) +{ Widget lw, ew, mbw; + Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNresizable, True); nargs++; + getoptWid = XtCreateWidget("getopt", formWidgetClass, parentWid, + args, nargs); + + nargs = 0; + lw = XtCreateManagedWidget("execlabel", labelWidgetClass, getoptWid, + args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromHoriz, lw); nargs++; + ew = XtCreateManagedWidget("execcommand", commandWidgetClass, + getoptWid, args, nargs); + getoptExec = False; + XtAddCallback(ew, XtNcallback, getoptExecCallback, NULL); + + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, lw); nargs++; + mbw = XtCreateManagedWidget("menubar", boxWidgetClass, getoptWid, + args, nargs); + CreateProgMenus(mbw, MenuItemCallback, NULL); +} + + +/* getstring dialogue */ + +static Widget getstringWid; + +static void CreateGetstringDialogue(Widget parentWid, Widget fromVertWid) +{ Widget valueWid; + Arg args[10]; + int nargs; + + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + getstringWid = XtCreateWidget("getstring", dialogWidgetClass, parentWid, + args, nargs); + XawDialogAddButton(getstringWid, "OK", + OKCallback, (XtPointer) getstringWid); + XawDialogAddButton(getstringWid, "Cancel", + CancelCallback,(XtPointer) getstringWid); + + /* + Prevent the value widget from resizing itself. + We cannot do this in the Xstaden default resources because + it is overriden when the dialog creates the value widget. + */ + valueWid = XtNameToWidget(getstringWid, "value"); + nargs = 0; + XtSetArg(args[nargs], XtNresizable, False); nargs++; + XtSetValues(valueWid, args, nargs); + +} + + +/* yesno dialogue */ + +static Widget yesnoWid; +static Widget yesnoLabelWid; + +static void CreateYesnoDialogue(Widget parentWid, Widget fromVertWid) +{ Widget yw, nw, cw; + Arg args[10]; + int nargs; + + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + yesnoWid = XtCreateWidget("yesno", formWidgetClass, parentWid, + args, nargs); + + + nargs = 0; + yesnoLabelWid = XtCreateManagedWidget("lab", labelWidgetClass, + yesnoWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, yesnoLabelWid); nargs++; + yw = XtCreateManagedWidget("YES", commandWidgetClass, + yesnoWid, args, nargs); + XtAddCallback(yw, XtNcallback, YESCallback, NULL); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, yesnoLabelWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, yw); nargs++; + nw = XtCreateManagedWidget("NO", commandWidgetClass, + yesnoWid, args, nargs); + XtAddCallback(nw, XtNcallback, NOCallback, NULL); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, yesnoLabelWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, nw); nargs++; + cw = XtCreateManagedWidget("Cancel", commandWidgetClass, + yesnoWid, args, nargs); + XtAddCallback(cw, XtNcallback, CancelCallback, NULL); +} + + + + +void CRAction(Widget wid, XEvent *event, + String *params, Cardinal *num_params) +/* + Action procedure to be called when CR is hit in a dialogue. + For dialogues which contain an "OK" widget, simulate a + pressing and callback of it. +*/ +{ + if (currentDialogueWid == nChoiceWid || + currentDialogueWid == getstringWid) + { dialogueResult = DialogueOK; + dialogueDone = True; + } + else if (currentDialogueWid == yesnoWid) + { dialogueResult = DialogueYES; + dialogueDone = True; + } +} + + + +/* ---- Exported routines ---- */ + + +Widget CreateDialogueShell(Widget toplevelWid) +/* + Create the dialogue shell as a child of `toplevelWid'. +*/ +{ Widget dialogueShellWid, qw, hw; + Arg args[10]; + int nargs; + XFontStruct *font; + signed int w, h; + + XtActionsRec actionTable[] = { {"CRAction", CRAction} }; + + + /* Add string to function bindings for our application actions */ + XtAppAddActions(XtWidgetToApplicationContext(toplevelWid), + actionTable, XtNumber(actionTable)); + + + nargs = 0; + dialogueShellWid = XtCreatePopupShell("Dialogue", topLevelShellWidgetClass, + toplevelWid, + args, nargs); + + dialogueFormWid = XtCreateManagedWidget("dialogueForm", formWidgetClass, + dialogueShellWid, + NULL, (Cardinal) 0); + + nargs = 0; + currFileWid = XtCreateManagedWidget("currFile", labelWidgetClass, + dialogueFormWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, currFileWid); nargs++; + currFuncWid = XtCreateManagedWidget("currFunc", labelWidgetClass, + dialogueFormWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, currFuncWid); nargs++; + hw = XtCreateManagedWidget("Help", commandWidgetClass, + dialogueFormWid, args, nargs); + XtAddCallback(hw, XtNcallback, HelpCallback, NULL); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, currFuncWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, hw); nargs++; + qw = XtCreateManagedWidget("quit", commandWidgetClass, + dialogueFormWid, args, nargs); + XtAddCallback(qw, XtNcallback, QuitAppCallback, NULL); + + + /* + There are many dialogues which can fit in here. + Create them all, but do not put them in yet. + */ + CreateNullDialogue(dialogueFormWid, hw); + CreateNChoiceDialogue(dialogueFormWid, hw); + CreateGetoptDialogue(dialogueFormWid, hw); + CreateGetstringDialogue(dialogueFormWid, hw); + CreateYesnoDialogue(dialogueFormWid, hw); + + + /* Put the null dialogue in by hand */ + XtManageChild(nullDialogueWid); + currentDialogueWid = nullDialogueWid; + + /* + * Set the size to be 80x14 text characters + */ + XtVaGetValues(currentDialogueWid, XtNfont, &font, NULL); + w = 136 - (19 * (font->max_bounds.width)); + w = 80 * font->max_bounds.width + (w>0?w:0); + h = 86 - 7 *(font->max_bounds.ascent + font->max_bounds.descent); + h = 15 *(font->max_bounds.ascent + font->max_bounds.descent) + h; + + XtVaSetValues(dialogueShellWid, + XtNwidth, w, + XtNheight, h, + NULL); + + dialogue_width = w; + dialogue_height = h; + + return(dialogueShellWid); +} + +/* --- C interface routines used by C, or FORTRAN (from userfacecom.c) --- */ + +/* + * Reads in a string from stdin. + * Args: + * prompt: obvious + * defval: default string (if user types in nothing) + * out : where to store the actual string read. + * outlen: sizeof(out) + * Returns: + * -1 : cancel + * 0 : ok + * 1 : ok, but used default (blank string if no default) + */ +int gtstr(char *prompt, char *defval, char *out, size_t outlen) { + char label[255]; + char *dialogueString; + int ret; + + /* + * Stick the label and default values in the getstring dialogue + */ + strcpy(label,prompt); + strcat(label," ?"); + + if (defval) + XtVaSetValues(getstringWid, XtNlabel, label, XtNvalue, defval, NULL); + else + XtVaSetValues(getstringWid, XtNlabel, label, XtNvalue, "", NULL); + + /* + Set the insertion point after the text + */ + XawTextSetInsertionPoint(XtNameToWidget(getstringWid, "value"), + (XawTextPosition)MAXLONG); + + switch (doDialogue(getstringWid)) { + case DialogueOK: + dialogueString = XawDialogGetValueString(getstringWid); + if (strlen(dialogueString) == 0) { + if (defval) + strncpy(out, defval, outlen); + else + *out = '\0'; + ret = 1; + } else { + strcpy(out, dialogueString); + ret = 0; + } + break; + case DialogueCancel: + *out = '\0'; + ret = -1; + break; + default:; + } + + dispatchCurrentEvents(); + return ret; +} + +/* + * Prompts the user with a yes/no question (defaults to yes). + * Args: + * prompt: what to ask. + * Returns: + * 0 = yes + * 1 = no + * -1 = cancel + */ +int yesno(char *prompt) { + char label[255]; + int ret; + + /* + Stick the prompt into the yesno widget + */ + strcpy(label, prompt); + strcat(label, " ?"); + XtVaSetValues(yesnoLabelWid, XtNlabel, label, NULL); + + switch (doDialogue(yesnoWid)) + { case DialogueYES: + ret = 0; + break; + case DialogueNO: + ret = 1; + break; + case DialogueCancel: + ret = -1; + break; + default:; + } + + dispatchCurrentEvents(); + return ret; +} + +/* + * Reads an integer in a given range from stdin. + * Args: + * minval: lower end of range (inclusive) + * maxval: upper end of range (inclusive) + * defval: default integer + * prompt: question to hassle user with + * status: returned status on validity of returned value + * 0 = ok + * -2 = quit + * -3 = error + * Returns: + * minval <= int <= maxval + */ +int getint(int minval, int maxval, int defval, char *prompt, int *status) { + char label[256], defVal[32]; + int ret; + DialogueResult dr; + char *resultString; + + /* + Generate the prompt and default value + */ + sprintf(label, "%s (%d -- %d) ?", prompt, minval, maxval); + sprintf(defVal, "%d", defval); + + /* + Stick them in the getstring widget + */ + XtVaSetValues(getstringWid, XtNlabel, label, XtNvalue, defVal, NULL); + + /* + Set the insertion point after the text + */ + XawTextSetInsertionPoint(XtNameToWidget(getstringWid, "value"), + (XawTextPosition)MAXLONG); + + do { + dr = doDialogue(getstringWid); + switch (dr) + { case DialogueOK: + resultString = XawDialogGetValueString(getstringWid); + ret = strtol(resultString,NULL,10); + *status = 0; + break; + + case DialogueCancel: + *status = -2; + break; + default:; + } + + } while(dr == DialogueOK && (ret > maxval || ret < minval)); + + dispatchCurrentEvents(); + return ret; +} + +/* + * Reads a float in a given range from stdin. + * Args: + * minval: lower end of range (inclusive) + * maxval: upper end of range (inclusive) + * defval: default float + * prompt: question to hassle user with + * status: returned status on validity of returned value + * 0 = ok + * -2 = quit + * -3 = error + * precision: how accurate to display the range and default values. + * Returns: + * minval <= float <= maxval + */ +float getfloat(float minval, float maxval, float defval, char *prompt, + int *status, int precision) { + char label[256], defVal[32]; + float ret; + DialogueResult dr; + char *resultString; + + /* + Generate the prompt and default value + */ + sprintf(label, "%s (%.*f -- %.*f) ?", prompt, precision, minval, + precision, maxval); + sprintf(defVal, "%.*f", precision, defval); + + /* + Stick them in the getstring widget + */ + XtVaSetValues(getstringWid, XtNlabel, label, XtNvalue, defVal, NULL); + + /* + Set the insertion point after the text + */ + XawTextSetInsertionPoint(XtNameToWidget(getstringWid, "value"), + (XawTextPosition)MAXLONG); + + do { + dr = doDialogue(getstringWid); + switch (dr) + { case DialogueOK: + resultString = XawDialogGetValueString(getstringWid); + ret = (float)atof(resultString); + *status = 0; + break; + + case DialogueCancel: + *status = -2; + break; + + default:; + } + + } while(dr == DialogueOK && (ret > maxval || ret < minval)); + + dispatchCurrentEvents(); + return ret; +} + +/* + * Displays a list of 'n' toggle-able items. An 'X' is displayed next to any + * currently selected items. User types in a number to toggle each item, or + * 0 to quit. + * Args: + * num : how many items to toggle + * prompts: list of names for each item + * bools : location of list of initial boolean states for items (set or + * unset). Also when returning, the final selected boolean states. + * Returns: + * -1 = cancel + * 0 = ok + */ +int checkn(int num, char **prompts, int **bools) { + int i, status; + Widget *toggles; + Boolean *states; + + toggles = (Widget *)malloc(num * sizeof(Widget)); + states = (Boolean *)malloc(num * sizeof(Boolean)); + + XtVaSetValues(nChoiceButtonsWid, XtNorientation, XtEhorizontal, NULL); + for (i=0; i numopts) + { fprintf(stderr, "Error in call to radion\n"); + return 1; + } + + toggles = (Widget *)malloc(numopts * sizeof(Widget)); + states = (Boolean *)malloc(numopts * sizeof(Boolean)); + + /* + Get the width of the surrounding dialogue. + We will set the width of the button box to be this, minus + a random amount to account for borders, spacing etc. + */ + XtVaGetValues(dialogueFormWid, XtNwidth, &dialogueWidth, NULL); + + /* + Put each toggle into nChoiceButtonsWid (a box) + */ + XawFormDoLayout(nChoiceWid, False); + for (i=0; i OK + KOPT=1 => dialogue wanted + + At the moment, the menu to display is hardwired via the generic + `progSpec.h' and associated `.c' file for each program. This + function should be rewritten so that it reads the menu data in + at run time, though it would almost certainly be best to do + this at initialisation time and then simply allow this function + to display the pre-built dialogue with the menu in it. +*/ +{ + showfu("menu selection"); + getopt_x(KBIN_p, KOPT_p, OPT_p); + if (helpindex[query_opt()].name) + showfu(helpindex[(int)*OPT_p].name); +} + +int bpause() { + XBell (GetDisplay(),100); + + /* flush and always succeed */ + updout_(); + return 0; +} diff --git a/src/staden/dialogues.h b/src/staden/dialogues.h new file mode 100644 index 0000000..66788d9 --- /dev/null +++ b/src/staden/dialogues.h @@ -0,0 +1,39 @@ +/* + Title: dialogues + + File: dialogues.h + Purpose: Routines to provide dialogue interaction + Last update: Wed Jun 27 1990 +*/ + + +/* + This module provides a series of routines to enable dialogue + interaction with a user. + + (They replace some routines removed from subs89). +*/ + + +/* + C (internal) - SUN FORTRAN (external) name conversions + This table is for the compiler specifically mentioned above, so + as well as being hostage to its horrors we can make use of its + advantages, such as long identifiers. +*/ + +/* ---- Includes ---- */ + +#include "fort.h" /* IMPORT: int_f definition */ +#include /* IMPORT: Widget */ + + + + +/* ---- Exports ---- */ + + +extern Widget CreateDialogueShell(Widget toplevelWid); +/* + Create the dialogue shell as a child of `toplevelWid'. +*/ diff --git a/src/staden/dias89.f b/src/staden/dias89.f new file mode 100644 index 0000000..db0e303 --- /dev/null +++ b/src/staden/dias89.f @@ -0,0 +1,2199 @@ +C DIASUB SUBROUTINES FOR DIAGON +C AUTHOR: RODGER STADEN +C 16-7-92 Now passing isame to qicks so that main diagonal can be ignored +C 16-7-92 Now passing isame to cfsq so that main diagonal can be ignored +C 7-2-90 mhist changed to using reals (rm and rmsq) for sd calc +C 8-2-90 switched idim1,idim2 in 2 places in mhist, once in qicks +C 14-2-90 removed call getreg from actout for compatibility with other +C programs +C 12-6-90 Changed all occurrences of lh to lf +C 9-7-90 removed menu routine +C 13-11-90 replaced all radio by radion +C 12-1-91 ALIGNM changed call to alignd to use nmax instead of maxseq +C Changed alignd to check for pout exceeding array bounds +C 25-4-91 Fixed bug in cfsq that allowed cfseq to extend off end of arrays +C 6-6-91 Added a check for pout over end of array to alignd +C added ctonum to pcon to allow for difference case letters +C 2-3-92 added filnam = ' ' for som ecalls to openf1 +C ROUTINES IN THIS LIBRARY: +C DMENU +C DIAPRW +C SHOBOX +C WRITAL +C ACTOUT +C DIAPER +C DIAPRO +C DIABOX +C DIAEXP +C DIAOBS +C DIALIN +C ALIGN +C FMT2 +C FRAME +C MOVEI +C HELPD +C FILEDG +C QVORH +C CFSQ +C ENCONB +C CFSEQ +C NCODE +C SETCON +C EXTNDM +C DSTAT +C REDEFD +C PRINTD +C MXPTHD +C SCORED +C APBIAS +C ACALCD +C GAPRM +C ALIGND + SUBROUTINE SWTCHD(SAME,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) + INTEGER SAME,OPT + CHARACTER HELPF*(*) + CALL YESNO(OPT,'Show main diagonal', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(OPT.LT.0) RETURN + SAME = 0 + IF(OPT.EQ.1) SAME = 1 + END + SUBROUTINE SWTCHI(MARKI,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) + INTEGER OPT + CHARACTER HELPF*(*) + CALL YESNO(OPT,'Plot identities in matching spans', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(OPT.LT.0) RETURN + MARKI = 1 + IF(OPT.EQ.1) MARKI = 0 + END +C DIAPRW + SUBROUTINE DIAPRW(S1M,S1P,IDIM1P,S2M,S2P,IDIM2P, + +M,IDM,LINEB, + +LINEC,LINEE,LENGTH,MINS,SEQVC,IDIM1,SEQHC,IDIM2, + +KBOUT,IDEV1,IV1,IH1,ISTARH,ISTARV,SAME) +C AUTHOR: RODGER STADEN + INTEGER S1M(IDIM1P),S1P(IDIM1P) + INTEGER S2M(IDIM2P),S2P(IDIM2P) + INTEGER LINEB(IDIM2P),LINEC(IDIM2P),M(IDM,IDM),LINEE(IDIM1P) + INTEGER TEMPB,TEMPP,SAME + CHARACTER SEQVC(IDIM1),SEQHC(IDIM2) +1 CONTINUE +C WRITE(KBOUT,1000) +C1000 FORMAT(' List matching spans') + CALL BUSY(KBOUT) + LB=LENGTH/2 + IDIM1T=IDIM1P-LENGTH + IDIM2T=IDIM2P-LENGTH +C NEED TO SET LINEB TO INITAL VALUE FOR FIRST LENGTH POSITIONS +C AND LINEE TO LEFT EDGE VALUES +C FIX LEFT EDGE BY SUMMING ALL THE VALUES FOR A LENGTH LENGTH/2 +C EITHER SIDE OF THE REAL SEQUENCE EDGE + DO 10 I=1,IDIM1T + LINEE(I)=0 + IM1 = I - 1 + DO 10 J=1,LENGTH + K = IM1 + J + LINEE(I)=LINEE(I)+M(S1M(K),S2M(J)) +10 CONTINUE +C NOW DO TOP EDGE + DO 20 I=1,IDIM2T +C SET UP POINTERS + LINEC(I)=0 + IM1 = I - 1 + DO 15 J=1,LENGTH + K = IM1 + J + LINEC(I)=LINEC(I)+M(S1M(J),S2M(K)) +15 CONTINUE +20 CONTINUE + DO 21 I=1,IDIM2T + LINEC(I)=LINEC(I+1) +21 CONTINUE + LINEC(IDIM2T)=0 +C MAIN LOOPS NOW +C LOOP FOR EACH ROW +C + ITV1P=LB+IV1-1-ISTARV+1 + ITV1M=IV1-LB-1-ISTARV+1 + ITH1P=LB+IH1-1-ISTARH+1 + ITH1M=IH1-LB-1-ISTARH+1 + ITV1MM=ITV1M+ISTARV-1 + ITH1MM=ITH1M+ISTARH-1 + DO 200 I=1,IDIM1T +C SET LINEB TO LINEC, THEN ZERO LINEC + DO 110 J=1,IDIM2T + LINEB(J+1)=LINEC(J) + LINEC(J)=0 +110 CONTINUE +C SET LINEB(1) TO EDGE VALUE AS ITS OFF PAGE + LINEB(1)=LINEE(I) +C +C NOW COMPARE THIS CHAR OF SEQ1 WITH WHOLE OF SEQ2 + TEMPP=S1P(I) + TEMPB=S1M(I) + DO 150 J=1,IDIM2T + LINEC(J)=LINEB(J)+M(TEMPP,S2P(J))-M(TEMPB,S2M(J)) + IF(LINEC(J).LT.MINS)GO TO 150 +C ABOVE CUTOFF SO WRITE + IF(I.EQ.J)THEN + IF(SAME.NE.1)THEN + WRITE(IDEV1,1006)I+ITV1MM + WRITE(IDEV1,1005)(SEQVC(K),K=MAX(1,I+ITV1M),MIN(IDIM1,I+ITV1P)) + WRITE(IDEV1,1005)(SEQHC(K),K=MAX(1,J+ITH1M),MIN(IDIM2,J+ITH1P)) + WRITE(IDEV1,1006)J+ITH1MM + END IF + ELSE + WRITE(IDEV1,1006)I+ITV1MM + WRITE(IDEV1,1005)(SEQVC(K),K=MAX(1,I+ITV1M),MIN(IDIM1,I+ITV1P)) + WRITE(IDEV1,1005)(SEQHC(K),K=MAX(1,J+ITH1M),MIN(IDIM2,J+ITH1P)) + WRITE(IDEV1,1006)J+ITH1MM + END IF +1006 FORMAT(' ',I7) +1005 FORMAT(' ',200A1) +150 CONTINUE +200 CONTINUE + END + SUBROUTINE SHOBOX(SEQVC,IDIMV,SEQHC,IDIMH,IWX1,IWX2,IWX3,IWX4, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT,KSTARH,KSTARV,IENDH,IENDV) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + CHARACTER SEQVC(IDIMV),SEQHC(IDIMH) + IF(0.EQ.0) GO TO 671 +1000 FORMAT(' Zoom-in to matrix',/) + WRITE(KBOUT,1000) +1002 FORMAT(' Horizontal sequence') +601 CONTINUE + WRITE(KBOUT,1002) + WRITE(KBOUT,10091) +10091 FORMAT(' MAX SIZE=36,DEFAULT=XHAIR POSITION - SPAN/2 TO', + +' XHAIR POSITION + SPAN/2') +C CALL FSTLST(IWB3,IWB4,KBIN,KBOUT) + IF(IWB3.LT.0)RETURN + IF(IWB4.LT.0)RETURN + IF((IWB3.EQ.0).AND.(IWB4.EQ.0))GO TO 651 + IF((IWB4-IWB3).GT.35)GO TO 601 + IF(IWB3.LT.KSTARH)GO TO 601 + IF(IWB3.GT.IENDH)GO TO 601 + IF(IWB4.GT.IENDH)GO TO 601 + IF(IWB3.GE.IWB4)GO TO 601 +C NE 0 SO USE + IWX3=IWB3 + IWX4=IWB4 +651 CONTINUE + IF((IWX4-IWX3).GT.35)GO TO 601 + IF(IWX3.LT.1)GO TO 601 + IF(IWX4.LT.IWX3)GO TO 601 +661 CONTINUE + WRITE(KBOUT,1001) +1001 FORMAT(' Vertical sequence') + WRITE(KBOUT,10091) +C CALL FSTLST(IWB1,IWB2,KBIN,KBOUT) + IF((IWB1.EQ.-99).OR.(IWB2.EQ.-99))THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 661 + END IF + IF(IWB1.LT.0)RETURN + IF(IWB2.LT.0)RETURN + IF((IWB1.EQ.0).AND.(IWB2.EQ.0))GO TO 671 + IF((IWB2-IWB1).GT.35)GO TO 661 + IF(IWB1.LT.KSTARV)GO TO 661 + IF(IWB1.GT.IENDV)GO TO 661 + IF(IWB2.GT.IENDV)GO TO 661 + IF(IWB1.GE.IWB2)GO TO 661 +C NE 0 SO USE + IWX1=IWB1 + IWX2=IWB2 +671 CONTINUE +C IF((IWX2-IWX1).GT.35)GO TO 661 +C IF(IWX1.LT.1)GOTO 661 +C IF(IWX2.LT.IWX1)GO TO 661 + IF(IWX1.LT.1) RETURN + IF(IWX2.LT.1) RETURN + IF(IWX3.LT.1) RETURN + IF(IWX4.LT.1) RETURN + IF(IWX1.GT.IDIMV) RETURN + IF(IWX2.GT.IDIMV) RETURN + IF(IWX3.GT.IDIMH) RETURN + IF(IWX4.GT.IDIMH) RETURN + IF(IWX2-IWX1.GT.35) RETURN + IF(IWX4-IWX3.GT.35) RETURN + CALL DIABOX(SEQVC(KSTARV),IDIMV,SEQHC(KSTARH), + +IDIMH,IWX1,IWX2,IWX3,IWX4,KBOUT) + RETURN + END + SUBROUTINE WRITAL(SEQHC,IDIMH,ISTARH,IENDH, + +SEQVC,IDIMV,ISTARV,IENDV,IDEVOT,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*),SEQHC(IDIMH),SEQVC(IDIMV) +C WRITE(KBOUT,*)' Write out aligned sequences' + IW3 = ISTARH + IW4 = IENDH + CALL GTREG(KBIN,KBOUT,ISTARH,IENDH,IW3,IW4, + +'Region of horizontal sequence', + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IW1 = ISTARV + IW2 = IENDV + CALL GTREG(KBIN,KBOUT,ISTARV,IENDV,IW1,IW2, + +'Region of vertical sequence', + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IDIML=MIN(IW2-IW1,IW4-IW3) + 1 + CALL FMT2(IDEVOT,SEQVC(IW1-ISTARV+1),SEQHC(IW3-ISTARH+1), + +IDIML,IW1,IW3) + RETURN + END + SUBROUTINE ACTOUT(SEQ,IDIM,ISTART,IEND,IDEVOT,FILOUT,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER FILOUT*(*),HELPF*(*),SEQ(IDIM) +C AUTHOR: RODGER STADEN +C FILE SEQUENCE TO DISK + FILOUT = ' ' + CALL OPENF1(IDEVOT,FILOUT,1,IOK,KBIN,KBOUT, + +'File name',IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + IW1 = ISTART + IW2 = IEND +C CALL GTREG(KBIN,KBOUT,ISTART,IEND,IW1,IW2, +C +'Region of sequence', +C +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C IF(IOK.NE.0) RETURN + IDIML=IW2-IW1+1 + CALL TITOUT(IDEVOT,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) + CALL FMTDK(IDEVOT,SEQ(IW1-ISTART+1),IDIML) + CLOSE(UNIT=IDEVOT) + RETURN + END +C +C DIAPER + SUBROUTINE DIAPER(SEQ1,IDIM1,SEQ2,IDIM2,LINEB,LINEC,IDL,MINS, + +MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX,KBOUT) +C AUTHOR: RODGER STADEN + INTEGER SEQ1(IDIM1),SEQ2(IDIM2) + INTEGER LINEB(IDL),LINEC(IDL) + INTEGER TEMPB + CALL BUSY(KBOUT) +C WRITE(KBOUT,*)'Working' + XMAX=IDIM2 + XMIN=1. + YMAX=IDIM1 + YMIN=1. + DO 10 I=1,IDIM2+1 + LINEB(I)=0 + LINEC(I)=0 +10 CONTINUE +C LOOP FOR EACH ROW + DO 200 I=1,IDIM1 +C ZERO LINE C AND SET LINEB TO LINEC + DO 110 J=1,IDIM2 + LINEB(J+1)=LINEC(J) +110 LINEC(J)=0 +C SET LINEB(1) TO ZERO AS ITS OFF PAGE + LINEB(1)=0 +C +C NOW COMPARE THIS CHAR OF SEQ1 WITH WHOLE OF SEQ2 + TEMPB=SEQ1(I) + DO 150 J=1,IDIM2 + IF(TEMPB.NE.SEQ2(J))GO TO 150 +C MATCH SO COUNT LENGTH BY ADDING CURRENT LENGTH OF THIS DIAGONAL + LINEC(J)=LINEB(J)+1 +C IS THIS HIGH ENOUGH? + IF(LINEC(J).LT.MINS)GO TO 150 + X=J + Y=I + CALL POINT(X,Y,XMAX,XMIN,YMAX,YMIN, + 1MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX) +150 CONTINUE +200 CONTINUE + RETURN + END +C +C DIAPRO + SUBROUTINE DIAPRO(S1M,S1P,IDIM1P,S2M,S2P,IDIM2P, + +M,IDM,LINEB, + +LINEC,LINEE,LENGTH,MINS, + +MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX,KBOUT,SAME) +C AUTHOR: RODGER STADEN + INTEGER S1M(IDIM1P),S1P(IDIM1P) + INTEGER S2M(IDIM2P),S2P(IDIM2P) + INTEGER LINEB(IDIM2P),LINEC(IDIM2P),M(IDM,IDM),LINEE(IDIM1P) + INTEGER TEMPB,TEMPP,SAME + CALL BUSY(KBOUT) +C WRITE(KBOUT,*)'Working' + IDIM1=IDIM1P-LENGTH + IDIM2=IDIM2P-LENGTH + XMAX=IDIM2 + XMIN=1. + YMAX=IDIM1 + YMIN=1. +C NEED TO SET LINEB TO INITAL VALUE FOR FIRST LENGTH POSITIONS +C AND LINEE TO LEFT EDGE VALUES +C FIX LEFT EDGE BY SUMMING ALL THE VALUES FOR A LENGTH LENGTH/2 +C EITHER SIDE OF THE REAL SEQUENCE EDGE + DO 10 I=1,IDIM1 + LINEE(I)=0 + IM1 = I - 1 + DO 9 J=1,LENGTH + K = IM1 + J + LINEE(I)=LINEE(I)+M(S1M(K),S2M(J)) +9 CONTINUE +10 CONTINUE +C NOW DO TOP EDGE + DO 20 I=1,IDIM2 +C SET UP POINTERS + LINEC(I)=0 + IM1 = I - 1 + DO 15 J=1,LENGTH + K = IM1 + J + LINEC(I)=LINEC(I)+M(S1M(J),S2M(K)) +15 CONTINUE +20 CONTINUE + DO 21 I=1,IDIM2 + LINEC(I)=LINEC(I+1) +21 CONTINUE + LINEC(IDIM2)=0 +C MAIN LOOPS NOW +C LOOP FOR EACH ROW +C + DO 200 I=1,IDIM1 +C SET LINEB TO LINEC, THEN ZERO LINEC + DO 110 J=1,IDIM2 + LINEB(J+1)=LINEC(J) + LINEC(J)=0 +110 CONTINUE +C SET LINEB(1) TO EDGE VALUE AS ITS OFF PAGE + LINEB(1)=LINEE(I) +C +C NOW COMPARE THIS CHAR OF SEQ1 WITH WHOLE OF SEQ2 + TEMPP=S1P(I) + TEMPB=S1M(I) + DO 150 J=1,IDIM2 + LINEC(J)=LINEB(J)+M(TEMPP,S2P(J))-M(TEMPB,S2M(J)) + IF(LINEC(J).LT.MINS)GO TO 150 +C ABOVE CUTOFF SO PLOT + IF(I.EQ.J)THEN + IF(SAME.NE.1)THEN + X=J + Y=I + CALL POINT(X,Y,XMAX,XMIN,YMAX,YMIN, + 1 MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX) + END IF + ELSE + X=J + Y=I + CALL POINT(X,Y,XMAX,XMIN,YMAX,YMIN, + 1 MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX) + END IF +150 CONTINUE +200 CONTINUE + END +C DIAPRI + SUBROUTINE DIAPRI(S1M,S1P,IDIM1P,S2M,S2P,IDIM2P, + +M,IDM,LINEB, + +LINEC,LINEE,LENGTH,MINS, + +MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX,KBOUT,SAME) +C AUTHOR: RODGER STADEN + INTEGER S1M(IDIM1P),S1P(IDIM1P) + INTEGER S2M(IDIM2P),S2P(IDIM2P) + INTEGER LINEB(IDIM2P),LINEC(IDIM2P),M(IDM,IDM),LINEE(IDIM1P) + INTEGER TEMPB,TEMPP,SAME +C VERSION TO MARK ALL IDENTITIES + CALL BUSY(KBOUT) + IDIM1=IDIM1P-LENGTH + IDIM2=IDIM2P-LENGTH + XMAX=IDIM2 + XMIN=1. + YMAX=IDIM1 + YMIN=1. +C NEED TO SET LINEB TO INITAL VALUE FOR FIRST LENGTH POSITIONS +C AND LINEE TO LEFT EDGE VALUES +C FIX LEFT EDGE BY SUMMING ALL THE VALUES FOR A LENGTH LENGTH/2 +C EITHER SIDE OF THE REAL SEQUENCE EDGE + DO 10 I=1,IDIM1 + LINEE(I)=0 + IM1 = I - 1 + DO 9 J=1,LENGTH + K = IM1 + J + LINEE(I)=LINEE(I)+M(S1M(K),S2M(J)) +9 CONTINUE +10 CONTINUE +C NOW DO TOP EDGE + DO 20 I=1,IDIM2 +C SET UP POINTERS + LINEC(I)=0 + IM1 = I - 1 + DO 15 J=1,LENGTH + K = IM1 + J + LINEC(I)=LINEC(I)+M(S1M(J),S2M(K)) +15 CONTINUE +20 CONTINUE + DO 21 I=1,IDIM2 + LINEC(I)=LINEC(I+1) +21 CONTINUE + LINEC(IDIM2)=0 +C MAIN LOOPS NOW +C LOOP FOR EACH ROW +C + DO 200 I=1,IDIM1 +C SET LINEB TO LINEC, THEN ZERO LINEC + DO 110 J=1,IDIM2 + LINEB(J+1)=LINEC(J) + LINEC(J)=0 +110 CONTINUE +C SET LINEB(1) TO EDGE VALUE AS ITS OFF PAGE + LINEB(1)=LINEE(I) +C +C NOW COMPARE THIS CHAR OF SEQ1 WITH WHOLE OF SEQ2 + TEMPP=S1P(I) + TEMPB=S1M(I) + DO 150 J=1,IDIM2 + LINEC(J)=LINEB(J)+M(TEMPP,S2P(J))-M(TEMPB,S2M(J)) + IF(LINEC(J).LT.MINS)GO TO 150 +C ABOVE CUTOFF SO PLOT + IF(I.EQ.J)THEN + IF(SAME.NE.1)THEN + K1 = I - 1 + K2 = J - 1 + DO 130 K = 1,LENGTH + K1 = K1 + 1 + K2 = K2 + 1 + IF(S1M(K1).EQ.S2M(K2))THEN + X = K2 + Y = K1 + CALL POINT(X,Y,XMAX,XMIN,YMAX,YMIN, + 1 MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX) + END IF +130 CONTINUE + END IF + ELSE + K1 = I - 1 + K2 = J - 1 + DO 140 K = 1,LENGTH + K1 = K1 + 1 + K2 = K2 + 1 + IF(S1M(K1).EQ.S2M(K2))THEN + X = K2 + Y = K1 + CALL POINT(X,Y,XMAX,XMIN,YMAX,YMIN, + 1 MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX) + END IF +140 CONTINUE + END IF +150 CONTINUE +200 CONTINUE + END +C ALIGN +C ROUTINE TO INSERT PADDING AS DASHES INTO DIAGON SEQS + SUBROUTINE ALIGN(SEQVV,IDIMV,SEQHH,IDIMH, + +MAXSEQ,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,ISTARV,IENDV,ISTARH,IENDH, + +FILEH,FILEV) + CHARACTER HELPF*(*),FILEH*(*),FILEV*(*) +C AUTHOR: RODGER STADEN + CHARACTER SEQVV(MAXSEQ),SEQHH(MAXSEQ) + CHARACTER VORH,DASH,IORD + PARAMETER (MAXPRM = 6) + CHARACTER PROMPT(2)*(MAXPRM) + SAVE DASH + DATA DASH/'-'/ +C ONLY ALLOW EDITING TO SEQUENCES THAT CAN BE TOTALLY CONTAINED IN THE +C RAM BUFFER, AND WHICH START AT 1 (WE DONT PLAN TO WRITE OUT TO THE +C DISK BUFFER) +C IE ISTARH=1, IDIMHISTART) + IF(I1.GT.ISTART)THEN +C NEED TO INSERT AT MOST SPAN/2 ELEMENTS + N=MIN(SPANO2+1,I1-ISTART) +C FIRST TO COPY? + J1=I1-1-ISTART+1 + K1=MSPO2 + DO 2 I=1,N + SEQN(K1)=CTONUM(SEQC(J1)) + J1=J1-1 + K1=K1-1 +2 CONTINUE + END IF +C DO RIGHT EDGE + DO 3 I=MSPO2+IDIMCA+1,MSPO2+IDIMCA+SPANO2 +3 SEQN(I)=IDCHR + IF(I2.LT.IEND)THEN + N=MIN(SPANO2,(IEND-I2)) + J1=I2+1-ISTART+1 + K1=MSPO2+1+IDIMCA + DO 4 I=1,N + SEQN(K1)=CTONUM(SEQC(J1)) + J1=J1+1 + K1=K1+1 +4 CONTINUE + END IF + RETURN + END + SUBROUTINE QVORH(VHOUT,IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT, + +FILEH,FILEV) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*),VHOUT,FILEH*(*),FILEV*(*) + PARAMETER (MAXPRM = 19) + CHARACTER PROMPT(2)*(MAXPRM) + PROMPT(1) = 'Horizontal sequence' + PROMPT(2) = 'Vertical sequence' + WRITE(KBOUT,1001)FILEH +1001 FORMAT(' Horizontal sequence is ',A) + WRITE(KBOUT,1002)FILEV +1002 FORMAT(' Vertical sequence is ',A) + VHOUT = ' ' + I = 1 + CALL RADION('Select sequence',PROMPT,2,I, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(I.LT.1) RETURN + IF(I.EQ.2) VHOUT = 'V' + IF(I.EQ.1) VHOUT = 'H' + END + SUBROUTINE CFSQ(SEQ1,IDIM1,SEQ2,IDIM2,POSN,WORDP,IDE,IDCHAR, + +CONSTS,LCONST,LENGTH,MINMAT, + +MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX,KBOUT,ISAME) +C 25-4-91 Fixed bug that allowed cfseq to extend off end of arrays +C by changing call to enconc from idim1 to idim1-minmat+1 +C +C NOTE !!!!!!! this "fix" does NOT work well for short sequences: +C enconc only process 1 to idim-length+1 so if idim = idim1-minmat+1 +C <= length-1 nothing gets hashed !!!!!!!!!! FIX IT sometime. +C should it be idim1-length+1 that gets sent to enconc ?? +C + INTEGER SEQ1(IDIM1),SEQ2(IDIM2) + INTEGER POSN(IDIM1),WORDP(IDE),CONSTS(0:LCONST) + CALL BUSY(KBOUT) + CALL SETCN(CONSTS,LENGTH,IDCHAR,LCONST) + CALL ENCONC(SEQ1,IDIM1-MINMAT+1,POSN,WORDP,IDE,IDCHAR, + +CONSTS,LENGTH,LCONST) + CALL CFSEQ(SEQ1,IDIM1,POSN,WORDP,IDE,SEQ2,IDIM2,CONSTS,LCONST, + +LENGTH,IDCHAR,MINMAT, + +MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX,ISAME) + END + SUBROUTINE CFSEQ(SEQ1,IDIM1,POSN,WORDP,IDE,SEQ2,IDIM2,CONSTS, + +LCONST, + +LENGTH,IDCHAR,MINMAT, + +MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX,ISAME) + INTEGER SEQ1(IDIM1),SEQ2(IDIM2) + INTEGER POSN(IDIM1),WORDP(IDE),CONSTS(0:LCONST) + INTEGER NCODEA,EXTNDM + EXTERNAL NCODEA,EXTNDM + LEX = MINMAT - LENGTH + XMAX=IDIM2 + XMIN=1. + YMAX=IDIM1 + YMIN=1. + DO 20 I = 1,IDIM2-MINMAT+1 + J = NCODEA(SEQ2(I),LENGTH,CONSTS,IDCHAR,LCONST) + IF(J.NE.0)THEN + J1 = WORDP(J) + IF(J1.NE.0)THEN + IF (((ISAME.EQ.1).AND.(I.NE.J1)).OR.(ISAME.EQ.0)) THEN + IMATCH = 0 + IF(MINMAT.GT.LENGTH) IMATCH = EXTNDM(SEQ1(J1+LENGTH), + + SEQ2(I+LENGTH),LEX) + IF(IMATCH.EQ.0)THEN + X=I + Y=J1 + CALL POINT(X,Y,XMAX,XMIN,YMAX,YMIN, + + MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX) + END IF + END IF +10 CONTINUE + J2 = J1 + J1 = POSN(J2) + IF(J1.NE.0)THEN + IF (((ISAME.EQ.1).AND.(I.NE.J1)).OR.(ISAME.EQ.0)) THEN + IMATCH = 0 + IF(MINMAT.GT.LENGTH) IMATCH = EXTNDM(SEQ1(J1+LENGTH), + + SEQ2(I+LENGTH),LEX) + IF(IMATCH.EQ.0)THEN + X=I + Y=J1 + CALL POINT(X,Y,XMAX,XMIN,YMAX,YMIN, + + MARGXL,MARGXR,MARGYL,MARGYR,ISXMAX,ISYMAX) + END IF + END IF + GO TO 10 + END IF + END IF + END IF +20 CONTINUE + END + INTEGER FUNCTION EXTNDM(SEQ1,SEQ2,LENGTH) + INTEGER SEQ1(LENGTH),SEQ2(LENGTH) + EXTNDM = 1 + DO 10 I = 1,LENGTH + IF(SEQ1(I).NE.SEQ2(I))RETURN +10 CONTINUE + EXTNDM = 0 + END + SUBROUTINE DSTAT(FILEH,ISH1,ISH2,FILEV,ISV1,ISV2, + +LENGTH,MINPRO,MINPER,KBOUT,ISAME,IMARK) + CHARACTER FILEH*(*),FILEV*(*) + WRITE(KBOUT,*)'Horizontal sequence' + WRITE(KBOUT,1023)FILEH + WRITE(KBOUT,10010)ISH1,ISH2 + WRITE(KBOUT,*)'Vertical sequence' + WRITE(KBOUT,1023)FILEV +1023 FORMAT(' ',A) + WRITE(KBOUT,10010)ISV1,ISV2 +10010 FORMAT(' Positions',/,' ',I6,' TO ',I6) + WRITE(KBOUT,10011)LENGTH +10011 FORMAT(' Span length=',I6) + WRITE(KBOUT,10014) +10014 FORMAT(' Scores') + WRITE(KBOUT,10012)MINPRO +10012 FORMAT(' Proportional=',I6) + WRITE(KBOUT,10013)MINPER +10013 FORMAT(' Identities=',I6) + IF(IMARK.EQ.1)THEN + WRITE(KBOUT,1001) +1001 FORMAT(' Identities on') + ELSE + WRITE(KBOUT,1002) +1002 FORMAT(' Identites off') + END IF + IF(ISAME.EQ.1)THEN + WRITE(KBOUT,1004) +1004 FORMAT(' Main diagonal blank') + ELSE + WRITE(KBOUT,1005) +1005 FORMAT(' Main diagonal shown') + END IF + END +C REDEFD + SUBROUTINE REDEFD(IDIMT,J1,J2,MAXSEQ,IDIMA,ISTART,IEND,IDIMB) +C AUTHOR RODGER STADEN +C DIAGON IS COMPLICATED BY HAVING SEVERAL ARRARY LIMITS +C THE SMALL MACHINE VERSION USES A DISK BUFFER +C ALL VERSIONS HAVE A CHUNK OF SEQUENCE IN RAM (ON THE LARGE MACHINE +C VERSION THIS WILL BE THE WHOLE SEQUENCE IF < MAXSEQ) +C ALL VERSIONS HAVE AN ACTIVE REGION FOR COMPARISON +C FOR ALL PROGRAMS OTHER THAN DIAGON THE ACTIVE REGION IS THE CHUNK IN THE +C RAM BUFFER, BUT FOR DIAGON IT MAY BE ONLY A PART OF THIS UP TO MAXCOM +C THIS CHUNK IS ALSO KEPT AS INTEGERS. +C THIS ROUTINE IS TO INITIALIZE THE ARRAY POINTERS AND SIZES +C MAXSEQ = THE DIMENSION OF THE RAM BUFFER SEQ +C IDIMT = THE ACTUAL SEQUENCE LENGTH (AND THEREFORE THE NUMBER OF ELEMENTS +C IN THE DISK BUFFER) +C ISTART = THE SEQUENCE NUMBER OF THE CHARACTER OCCUPYING SEQ(1) +C J1 = THE SEQUENCE NUMBER OF THE FIRST CHARACTER IN THE ACTIVE REGION +C J2 = THE SEQUENCE NUMBER OF THE LAST CHARACTER IN THE ACTIVE REGION +C IDIMA = J2-J1+1 I.E. THE NUMBER OF ELEMENTS IN THE ACTIVE REGION +C IEND = THE SEQUENCE NUMBER OF THE LAST ELEMENT OF SEQ +C IDIMB = IEND-ISTART+1 I.E. THE NUMBER OF ELEMENTS IN THE RAM BUFFER + K1 = 0 + K2 = 0 + IF(K1.EQ.0)K1=J1 + K2MAX=MIN((K1+MAXSEQ-1),IDIMT) + IF(K2.GT.K2MAX)K2=K2MAX + IF(K2.EQ.0)K2=MIN(J2,K2MAX) + J1=K1 + J2=K2 + IDIMA=J2-J1+1 + IDIMB=IEND-ISTART+1 + END + SUBROUTINE MATTIN(MATRIX,IDM,FILNAM,FILEP,CHRSET, + +KBIN,KBOUT,IDEV,MATMAX,IHELPS,IHELPE,HELPF,IDEVH) + INTEGER MATRIX(IDM,IDM) + CHARACTER FILEP*(*),FILNAM*(*),CHRSET(IDM),HELPF*(*) + PARAMETER (MAXPRM = 20) + CHARACTER PROMPT(3)*(MAXPRM) + IN = 1 + PROMPT(1) = 'Identity matrix' + PROMPT(2) = 'MDM78 matrix' + PROMPT(3) = 'Personal matrix file' +10 CONTINUE + CALL RADION('Select score matrix',PROMPT,3,IN, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IN.LT.1) RETURN + IF(IN.EQ.1)THEN +C SET IDENTITIES + DO 15 I = 1,IDM + DO 15 J = 1,IDM + IF(I.EQ.J)THEN + MATRIX(I,J) = 1 + ELSE + MATRIX(I,J) = 0 + END IF +15 CONTINUE + ELSE IF(IN.EQ.2)THEN +C READ IN MDM78 + CALL GETMAT(IDEV,FILEP,MATRIX,IDM,CHRSET,KBOUT,IOK) + IF(IOK.NE.0)GO TO 100 + ELSE IF(IN.EQ.3)THEN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + + 'Matrix file name', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)GO TO 10 + CALL GETMAT(IDEV,FILNAM,MATRIX,IDM,CHRSET,KBOUT,IOK) + IF(IOK.NE.0)GO TO 100 + ELSE + GO TO 10 + END IF +C NEED TO KNOW LARGEST SCORE IN MATRIX FOR STATS + MATMAX=0 + DO 20 I=1,IDM + DO 20 J=1,IDM + IF(MATMAX.LT.MATRIX(I,J))MATMAX=MATRIX(I,J) +20 CONTINUE + MATMAX=MATMAX+1 + WRITE(KBOUT,1004) +1004 FORMAT(' Remember to reset the score for',/, + + ' the proportional algorithm') + RETURN +100 CONTINUE + CALL ERROM(KBOUT,'Error in score matrix file') + GO TO 10 + END + SUBROUTINE ALIGNM(SEQ1N,SEQ2N,IDIM1,IDIM2,CC,DD,RR,SS,SOP, + +NMAX,IV1,IH1,IDIMV,IDIMH,MAXSEQ,WTS,IDM,ISCORE,IG,IH,KBIN, + +KBOUT,IDEV,SEQ1,SEQ2,SEQ1A,SEQ2A, + +IHELPS,IHELPE,HELPF,IDEVH,KEEP,STACK,MAXSTK,STKREC,IOK) + INTEGER CC(0:NMAX+1),DD(0:NMAX+1),RR(0:NMAX+1),SS(0:NMAX+1) + INTEGER WTS(0:IDM,0:IDM),SOP(0:2*NMAX),R1,R2 + INTEGER SEQ1N(NMAX),SEQ2N(NMAX),STKREC,STACK(0:MAXSTK) + CHARACTER SEQ1(MAXSEQ),SEQ2(MAXSEQ),SEQ1A(NMAX),SEQ2A(NMAX) + CHARACTER HELPF*(*),PAD + SAVE PAD + DATA PAD/','/ +C +C 12-1-91 modified call to alignd to send nmax instead of maxseq +C +C MN = 1 +C MX = NMAX +C WINDOW = NMAX +C CALL GETINT(MN,MX,WINDOW,'Window size', +C +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) +C IF(IOK.NE.0) RETURN +C WINDOW = IVAL +C10 CONTINUE + CALL BUSY(KBOUT) + LW1 = MIN(IDIM1,NMAX) + LW2 = MIN(IDIM2,NMAX) + N = MAX(LW1,LW2) + CALL DIFF(SEQ1N,SEQ2N,LW1,LW2, + +CC,DD,RR,SS,SOP,N, + +WTS,IDM,ISCORE,IG,IH,KBOUT,STACK,MAXSTK,STKREC,IOK) + IF(IOK.NE.0) RETURN + CALL ALIGND(SEQ1(IV1),SEQ2(IH1),SOP, + +SEQ1A,SEQ2A,N,NMAX,R1,R2,PAD,NP1,NP2,IDIM1,IDIM2) + KPOUT = MAX(R1,R2) + PC = PCON(SEQ1A,SEQ2A,KPOUT,PAD) + CALL FMT2(IDEV,SEQ1A,SEQ2A,KPOUT,IV1,IH1) + WRITE(IDEV,1001)PC +1001 FORMAT(' Conservation ',F5.1,'%') + WRITE(IDEV,1002)NP1,NP2 +1002 FORMAT(' Number of padding characters inserted',I6,' and',I6) + CALL YESNO(KEEP,'Keep alignment', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(KEEP.LT.0) RETURN + IF(KEEP.EQ.1) RETURN + NMOV = KPOUT - IDIM1 + IV2 = IV1 + IDIM1 + CALL MOVEC(SEQ1,MAXSEQ,IDIMV,IV2,NMOV) + IDIMV = IDIMV + NMOV + CALL SQCOPY(SEQ1A,SEQ1(IV1),KPOUT) + NMOV = KPOUT - IDIM2 + IV2 = IH1 + IDIM2 + CALL MOVEC(SEQ2,MAXSEQ,IDIMH,IV2,NMOV) + IDIMH = IDIMH + NMOV + CALL SQCOPY(SEQ2A,SEQ2(IH1),KPOUT) + END + SUBROUTINE ALIGND(SEQ1,SEQ2,S,SEQ1A,SEQ2A,NMAX,MAXSEQ, + +R1,R2,PAD,NP1,NP2,IDIM1,IDIM2) + CHARACTER SEQ1(MAXSEQ),SEQ2(MAXSEQ),SEQ1A(MAXSEQ),SEQ2A(MAXSEQ) + CHARACTER PAD + INTEGER S(0:2*NMAX),P1,P2,POUT,R1,R2 +C 12-1-91 Added checks for pout going off end of array +C 6-6-91 Added another check! + P1 = 1 + P2 = 1 + NP1 = 0 + NP2 = 0 + POUT = 1 + I = -1 +10 CONTINUE + IF((P1.LE.IDIM1).AND.(P2.LE.IDIM2).AND.(POUT.LE.MAXSEQ)) THEN + I = I + 1 + IF(S(I).EQ.0) THEN +C WRITE(*,*)P1,'=',P2,SEQ1(P1),SEQ2(P2) + SEQ1A(POUT) = SEQ1(P1) + SEQ2A(POUT) = SEQ2(P2) + P1 = P1 + 1 + P2 = P2 + 1 + POUT = POUT + 1 + ELSE + IF(S(I).LT.0) THEN + K = ABS(S(I)) +C WRITE(*,*)'INSERT ',K,' AT',P2 + CALL SQCOPY(SEQ1(P1),SEQ1A(POUT),K) + CALL FILLC(SEQ2A(POUT),K,PAD) + NP2 = NP2 + K + P1 = P1 + K + POUT = POUT + K + ELSE + K = S(I) +C WRITE(*,*)'INSERT ',K,' AT',P1 + CALL SQCOPY(SEQ2(P2),SEQ2A(POUT),K) + CALL FILLC(SEQ1A(POUT),K,PAD) + NP1 = NP1 + K + P2 = P2 + K + POUT = POUT + K + END IF + END IF + GO TO 10 + END IF + R1 = POUT - 1 + R2 = POUT - 1 + J = 0 + K = 0 + IF((P1.LE.IDIM1).AND.(POUT.LE.MAXSEQ)) THEN + J = IDIM1 - P1 + 1 + J = MIN(J,MAXSEQ-POUT+1) + CALL SQCOPY(SEQ1(P1),SEQ1A(POUT),J) + R1 = R1 + J + END IF + IF((P2.LE.IDIM2).AND.(POUT.LE.MAXSEQ)) THEN + K = IDIM2 - P2 + 1 + K = MIN(K,MAXSEQ-POUT+1) + CALL SQCOPY(SEQ2(P2),SEQ2A(POUT),K) + R2 = R2 + K + END IF + I = R1 - R2 + IF(I.GT.0) THEN + CALL FILLC(SEQ2A(POUT+K),I,PAD) + ELSE IF(I.LT.0) THEN + I = ABS(I) + CALL FILLC(SEQ1A(POUT+J),I,PAD) + END IF + END + REAL FUNCTION PCON(SEQ1,SEQ2,L,PAD) + CHARACTER SEQ1(L),SEQ2(L),PAD + INTEGER CTONUM + EXTERNAL CTONUM + N = 0 + DO 10 I = 1,L + IF(SEQ1(I).EQ.PAD) SEQ1(I) = '-' + IF(SEQ2(I).EQ.PAD) SEQ2(I) = '-' + IF(CTONUM(SEQ1(I)).EQ.CTONUM(SEQ2(I))) N = N + 1 +10 CONTINUE + PCON = 100. * REAL(N)/REAL(L) + END + SUBROUTINE DIFF(SA,SB,IDIM1,IDIM2,CC,DD,RR,SS,SOP,NMAX, + +W,IDM,SCORE,G,H,KBOUT,STACK,MAXSTK,STKREC,IOK) + IMPLICIT INTEGER(A-Z) + INTEGER CC(0:NMAX+1),DD(0:NMAX+1),RR(0:NMAX+1),SS(0:NMAX+1) + INTEGER W(0:IDM,0:IDM),SOP(0:2*NMAX),STACK(0:MAXSTK) + INTEGER SA(NMAX),SB(NMAX) + EXTERNAL GAP + IOK = 0 + CALL FILLI(SOP,2*NMAX+1,0) + M = IDIM1 + N = IDIM2 + LM = G + H + LAST = 0 + SAPP = 0 + A = 1 + B = 1 + TB = G + TE = H + MIDI = 0 + MIDJ = 0 + MIDC = 0 + TYPE = 0 +C INITIALISE THE STACK WITH A PUSH + CALL STACKH(1,A,B,M,N,TB,TE,MIDI,MIDJ,TYPE,MIDC,990, + +STACK,MAXSTK,STKREC,IOK) + IF(IOK.NE.0) GO TO 999 +90 CONTINUE + IF(N.LE.0)THEN + IF(M.GT.0) CALL DEL(M,LAST,SOP,NMAX,SAPP) + ANS = GAP(M,G,H) + GO TO 980 + END IF + IF(M.LE.1) THEN + IF(M.LE.0) THEN + CALL INS(N,LAST,SOP,NMAX,SAPP) + ANS = GAP(N,G,H) + GO TO 980 + END IF + IF(TB.GT.TE) TB = TE + MIDC = TB + H + GAP(N,G,H) + MIDJ = 0 + ITP = B - 1 + DO 100 J = 1,N + C = GAP(J-1,G,H) + W(SA(A),SB(J+ITP)) + GAP(N-J,G,H) + IF (C.LT.MIDC) THEN + MIDC = C + MIDJ = J + END IF +100 CONTINUE + IF(MIDJ.EQ.0)THEN + CALL INS(N,LAST,SOP,NMAX,SAPP) + CALL DEL(1,LAST,SOP,NMAX,SAPP) + ELSE + IF(MIDJ.GT.1) CALL INS(MIDJ-1,LAST,SOP,NMAX,SAPP) + CALL REP(LAST,SOP,NMAX,SAPP) + IF(MIDJ.LT.N) CALL INS(N-MIDJ,LAST,SOP,NMAX,SAPP) + END IF + ANS = MIDC + GO TO 980 + END IF + MIDI = M/2 +C FORWARD PHASE + CC(0) = 0 + T = G + DO 200 J=1,N + T = T + H + CC(J) = T + DD(J) = T + G +200 CONTINUE + T = TB + JTP = B - 1 + DO 400 I = 1,MIDI + S = CC(0) + T = T + H + C = T + CC(0) = C + E = T + G + ITP = I + A - 1 + DO 300 J = 1,N + C = C + LM + E = E + H + IF(C.LT.E) E = C + C = CC(J) + LM + D = DD(J) + H + IF(C.LT.D) D = C + C = S + W(SA(ITP),SB(J+JTP)) + IF(E.LT.C) C = E + IF(D.LT.C) C = D + S = CC(J) + CC(J) = C + DD(J) = D +300 CONTINUE +400 CONTINUE + DD(0) = CC(0) +C REVERSE PHASE + RR(N) = 0 + T = G + DO 500 J =N-1,0,-1 + T = T + H + RR(J) = T + SS(J) = T + G +500 CONTINUE + T = TE + DO 700 I=M-1,MIDI,-1 + S = RR(N) + T = T + H + C = T + RR(N) = C + E = T + G + ITP = I + A + DO 600 J = N-1,0,-1 + C = C + LM + E = E + H + IF(C.LT.E) E = C + C = RR(J) + LM + D = SS(J) + H + IF(C.LT.D) D = C + C = S + W(SA(ITP),SB(J+B)) + IF(E.LT.C) C = E + IF(D.LT.C) C = D + S = RR(J) + RR(J) = C + SS(J) = D +600 CONTINUE +700 CONTINUE + SS(N) = RR(N) +C FIND OPTIMAL MIDPOINT + MIDC = CC(0) + RR(0) + MIDJ = 0 + TYPE = 1 + DO 800 J = 0,N + C = CC(J) + RR(J) + IF(C.LE.MIDC) THEN + IF((C.LT.MIDC).OR.(CC(J).NE.DD(J)).AND.(RR(J).EQ.SS(J)))THEN + MIDC = C + MIDJ = J + END IF + END IF +800 CONTINUE + DO 900 J = N,0,-1 + C = DD(J) + SS(J) - G + IF(C.LT.MIDC) THEN + MIDC = C + MIDJ = J + TYPE = 2 + END IF +900 CONTINUE +C CONQUER RECURSIVELY AROUND MIDPOINT + IF(TYPE.NE.1) GO TO 960 + IF(TYPE.EQ.1) THEN + CALL STACKH(1,A,B,M,N,TB,TE,MIDI,MIDJ,TYPE,MIDC,950, + +STACK,MAXSTK,STKREC,IOK) + IF(IOK.NE.0) GO TO 999 + M = MIDI + N = MIDJ + TE = G + GO TO 90 + END IF +950 CONTINUE + CALL STACKH(1,A,B,M,N,TB,TE,MIDI,MIDJ,TYPE,MIDC,980, + +STACK,MAXSTK,STKREC,IOK) + IF(IOK.NE.0) GO TO 999 + A = A + MIDI + B = B + MIDJ + M = M - MIDI + N = N - MIDJ + TB = G + GO TO 90 +960 CONTINUE + CALL STACKH(1,A,B,M,N,TB,TE,MIDI,MIDJ,TYPE,MIDC,970, + +STACK,MAXSTK,STKREC,IOK) + IF(IOK.NE.0) GO TO 999 + M = MIDI - 1 + N = MIDJ + TE = 0 + GO TO 90 +970 CONTINUE + CALL DEL(2,LAST,SOP,NMAX,SAPP) + CALL STACKH(1,A,B,M,N,TB,TE,MIDI,MIDJ,TYPE,MIDC,980, + +STACK,MAXSTK,STKREC,IOK) + IF(IOK.NE.0) GO TO 999 + A = A + MIDI + 1 + B = B + MIDJ + M = M - MIDI - 1 + N = N - MIDJ + TB = 0 + GO TO 90 +980 CONTINUE + SCORE = MIDC + CALL STACKH(2,A,B,M,N,TB,TE,MIDI,MIDJ,TYPE,MIDC,ADDR, + +STACK,MAXSTK,STKREC,IOK) + IF(IOK.NE.0) GO TO 999 + IF(ADDR.EQ.950) GO TO 950 + IF(ADDR.EQ.970) GO TO 970 + IF(ADDR.EQ.980) GO TO 980 + IF(ADDR.EQ.990) GO TO 990 + WRITE(KBOUT,*)'Unexpected address in align' + IOK = 4 + RETURN +999 CONTINUE + IF(IOK.EQ.1) THEN + WRITE(KBOUT,*)'Stack overflow' + ELSE IF(IOK.EQ.2) THEN + WRITE(KBOUT,*)'Stack underflow' + ELSE IF (IOK.EQ.3) THEN + WRITE(KBOUT,*)'Unexpected stack task' + END IF +990 CONTINUE + END + SUBROUTINE GETGAP(KBIN,KBOUT,IG,IH, + +IHELPS,IHELPE,HELPF,IDEVH,IDM,IOK) + CHARACTER HELPF*(*) +C FOR GAP OF LENGTH K, COST IS G + H*K +C WHERE G IS COST OF STARTING GAP, AND H IS ADDED FOR EACH ELEMENT IN GAP +C WRITE(KBOUT,*)'THE COST OF A GAP OF LENGTH K = G + H*K' + MN = 1 + MX = 100 + IG = 10 + IF(IDM.EQ.5) IG = 20 + CALL GETINT(MN,MX,IG,'Penalty for starting a gap', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IG = IVAL + MN = 1 + MX = 100 + IH = 10 + IF(IDM.EQ.5) IH = 5 + CALL GETINT(MN,MX,IH,'Penalty for each residue in gap', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IH = IVAL + END + INTEGER FUNCTION GAP(K,G,H) + INTEGER K,G,H +C NOTE FOR DES: HE HAS EQ HERE + IF(K.LE.0) THEN + GAP = 0 + ELSE + GAP = G + H * K + END IF + END + SUBROUTINE DEL(K,LAST,S,NMAX,SAPP) + INTEGER S(0:NMAX*2),SAPP + IF(LAST.LT.0) THEN + S(SAPP-1) = S(SAPP-1) - K + LAST = -K + ELSE + S(SAPP) = -K + SAPP = SAPP + 1 + LAST = -K + END IF + END + SUBROUTINE INS(K,LAST,S,NMAX,SAPP) + INTEGER S(0:NMAX*2),SAPP + IF (LAST.LT.0) THEN + S(SAPP-1) = K + S(SAPP) = LAST +C DES HAS NEXT LINE. I REMOVED IT 16-5-89 +C LAST = K + SAPP = SAPP + 1 + ELSE + S(SAPP) = K + SAPP = SAPP + 1 + LAST = K + END IF + END + SUBROUTINE REP(LAST,S,NMAX,SAPP) + INTEGER S(0:NMAX*2),SAPP + S(SAPP) = 0 + SAPP = SAPP + 1 + LAST = 0 + END + SUBROUTINE STACKH(JOB,A,B,M,N,TB,TE,MIDI,MIDJ,TYPE,MIDC,ADDR, + +STACK,MAXSTK,STKREC,IOK) + IMPLICIT INTEGER (A-Z) + INTEGER STACK(0:MAXSTK) + SAVE + DATA SP/-1/ +C HANDLE STACK: 1 = PUSH, 2 = POP + IF(JOB.EQ.1) THEN + SP = SP + STKREC + IF(SP.GT.MAXSTK) THEN +C WRITE(*,*)'HELP, STACK OVERFLOW' + IOK = 1 + RETURN + END IF + STACK(SP-10) = A + STACK(SP-9) = B + STACK(SP-8) = M + STACK(SP-7) = N + STACK(SP-6) = TB + STACK(SP-5) = TE + STACK(SP-4) = MIDI + STACK(SP-3) = MIDJ + STACK(SP-2) = TYPE + STACK(SP-1) = MIDC + STACK(SP) = ADDR + ELSE IF(JOB.EQ.2) THEN + SP = SP - STKREC + IF(SP.LT.-1) THEN +C WRITE(*,*)'HELP, STACK UNDERFLOW' + IOK = 2 + RETURN + END IF + A = STACK(SP+1) + B = STACK(SP+2) + M = STACK(SP+3) + N = STACK(SP+4) + TB = STACK(SP+5) + TE = STACK(SP+6) + MIDI = STACK(SP+7) + MIDJ = STACK(SP+8) + TYPE = STACK(SP+9) + MIDC = STACK(SP+10) + ADDR = STACK(SP+11) + ELSE +C WRITE(*,*)'HELP, STACK COCKUP' + IOK = 3 +C STOP + END IF + END + SUBROUTINE PAMDIS(MATRIX,WTS,IDM) + INTEGER MATRIX(IDM,IDM),WTS(0:IDM,0:IDM) + IF(IDM.EQ.26) THEN + N = 8 + M = 0 + DO 10 I = 1,IDM + DO 5 J = 1,IDM + K = MATRIX(I,J) + M = MAX(M,K) +5 CONTINUE +10 CONTINUE + DO 20 I = 1,IDM + DO 15 J = 1,IDM + K = M - MATRIX(I,J) + WTS(I,J) = K +15 CONTINUE +20 CONTINUE + DO 30 I = 0,IDM + WTS(I,0) = N + WTS(0,I) = N +30 CONTINUE + RETURN + END IF + DO 40 I = 1,IDM + DO 40 J = 1,IDM + IF(I.EQ.J) THEN + WTS(I,J) = 0 + ELSE + WTS(I,J) = 20 + END IF +40 CONTINUE + DO 50 I = 0,IDM + WTS(I,0) = 10 + WTS(0,I) = 10 +50 CONTINUE + END + SUBROUTINE ENCONC(SEQ,IDIM,POSN,WORDP,IDE,IDCHAR,CONSTS,LENGTH, + +LCONST) +C AUTHOR RODGER STADEN + INTEGER SEQ(IDIM) + INTEGER POSN(IDIM),WORDP(IDE),CONSTS(0:LCONST) + INTEGER NCODEA + EXTERNAL NCODEA +C ENCODES A SEQUENCE OF LENGTH IDIM AND CHARACTERSET SIZE IDCHAR +C INTO TWO ARRAYS: WORDP(I) CONTAINS THE POSITION OF THE FIRST OCCURRENCE +C OF WORD(I), POSN(I) CONTAINS A LINKED LIST OF SECOND, THIRD,... OCCURENCES +C OF WORD + CALL FILLI(POSN,IDIM,0) + CALL FILLI(WORDP,IDE,0) + DO 20 I = 1, IDIM-LENGTH+1 + J = NCODEA(SEQ(I),LENGTH,CONSTS,IDCHAR,LCONST) + IF(J.NE.0)THEN + J1 = WORDP(J) + IF(J1.EQ.0)THEN + WORDP(J) = I + ELSE +10 CONTINUE + J2 = J1 + J1 = POSN(J2) + IF(J1.NE.0) GO TO 10 + POSN(J2) = I + END IF + END IF +20 CONTINUE + END + SUBROUTINE QICKS(SEQ1,IDIM1,POSN,WORDP,IDE,SEQ2,IDIM2,CONSTS, + +LENGTH,IDM,LCONST,HIST,MAXSEQ,MATRIX,SEQS,MAIND, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,SPAN,MINPRO,KBOUT,RNSD, + +ISAME) + INTEGER SEQ1(IDIM1),SEQ2(IDIM2),SEQS(IDIM1),SPAN + INTEGER POSN(IDIM1),WORDP(IDE),CONSTS(0:LCONST) + INTEGER MAIND(IDM) + INTEGER HIST(-MAXSEQ:MAXSEQ) + PARAMETER (MAXDIA = 20) + INTEGER TOPD(MAXDIA),TOPI(MAXDIA),TOPJ(MAXDIA),MATRIX(IDM,IDM) + EXTERNAL NCODEA + NDIAG = MAXDIA + CALL BUSY(KBOUT) + CALL FILLI(HIST(-IDIM1),IDIM2+IDIM1+1,0) + CALL SETCN(CONSTS,LENGTH,IDM,LCONST) +C WRITE(*,*)'CONSTS' +C WRITE(*,*)CONSTS + CALL ENCONC(SEQ1,IDIM1,POSN,WORDP,IDE,IDM,CONSTS,LENGTH, + +LCONST) + DO 4 I = 1,IDM + MAIND(I) = MATRIX(I,I) +4 CONTINUE +C WRITE(*,*)'MAIND' +C WRITE(*,*)MAIND + CALL WDSCR(SEQ1,SEQS,IDIM1,LENGTH,MAIND,IDM) +C WRITE(*,*)'SEQS' +C WRITE(*,*)(SEQS(K),K=1,30) +C WRITE(*,*)'SEQ1' +C WRITE(*,*)(SEQ1(K),K=1,30) +C WRITE(*,*)IDIM1,IDIM2,LENGTH + DO 20 I = 1,IDIM2-LENGTH+1 + J = NCODEA(SEQ2(I),LENGTH,CONSTS,IDM,LCONST) + IF(J.NE.0)THEN + J1 = WORDP(J) + IF(J1.NE.0)THEN + K = I - J1 +C FOR IDENTITIES ADD 1 ON NEXT LINE (NOT SEQS) + HIST(K) = HIST(K) + SEQS(J1) +10 CONTINUE + J2 = J1 + J1 = POSN(J2) + IF(J1.NE.0)THEN + K = I - J1 +C FOR IDENTITIES ADD 1 ON NEXT LINE (NOT SEQS) + HIST(K) = HIST(K) + SEQS(J1) + GO TO 10 + END IF + END IF + END IF +20 CONTINUE + IF (ISAME.EQ.1) HIST(0) = 0 + CALL MHIST(HIST,IDIM1,IDIM2,TOPD,TOPI,TOPJ, + +NDIAG,MAXSEQ,RNSD) + IF(NDIAG.EQ.0) THEN + WRITE(KBOUT,*)' No diagonals found scoring',RNSD, + + ' sd above mean' + RETURN + END IF + ISPO2 = SPAN/2 + XMIN = 1. + XMAX = IDIM2 + YMIN = 1. + YMAX = IDIM1 + CALL VECTOM + DO 40 I = 1,NDIAG + IF(TOPD(I).NE.0) THEN + L = MIN(IDIM1-TOPJ(I),IDIM2-TOPI(I)) + 1 + CALL DSCORP(SEQ1(TOPJ(I)),SEQ2(TOPI(I)),L,MATRIX,IDM, + + SPAN,MINPRO,ISPO2,TOPJ(I),TOPI(I), + +XMAX,XMIN,YMAX,YMIN,MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF +40 CONTINUE + CALL VT100M + END + SUBROUTINE DSCORP(SEQ1,SEQ2,L,MATRIX,IDM,SPAN,MINSCR,SPO2,JS,IS, + +XMAX,XMIN,YMAX,YMIN,MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + INTEGER SEQ1(L),SEQ2(L),MATRIX(IDM,IDM),SPAN,FRONT,BACK,SPO2 +C 8-6-91 Fixed bug that allowed span>L + M = 0 + FRONT = SPAN + BACK = 0 + DO 10 I = 1,MIN(SPAN,L) + M = M + MATRIX(SEQ1(I),SEQ2(I)) +10 CONTINUE + IF(M.GE.MINSCR) THEN + Y = JS + SPO2 + X = IS + SPO2 +C WRITE(*,*)IX,IY + CALL POINT(X,Y,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + DO 20 I = 2,L-SPAN+1 + FRONT = FRONT + 1 + BACK = BACK + 1 + MM = MATRIX(SEQ1(BACK),SEQ2(BACK)) + MP = MATRIX(SEQ1(FRONT),SEQ2(FRONT)) + M = M - MM + MP + IF(M.GE.MINSCR) THEN + Y = JS + BACK + SPO2 + X = IS + BACK + SPO2 +C WRITE(*,*)IX,IY + CALL POINT(X,Y,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF +20 CONTINUE + END + SUBROUTINE MHIST(HIST,IDIM1,IDIM2,TOPD,TOPI,TOPJ,NDIAG, + + MAXSEQ,RNSD) + INTEGER HIST(-MAXSEQ:MAXSEQ),TOPD(NDIAG),TOPI(NDIAG),TOPJ(NDIAG) +C ANALYSE HISTOGRAM TO FIND BEST NDIAG DIAGONALS +C LOOK AT THE TOP TEN SCORES, TOP DOWN +C IF ELEMENT I > TOP(J) THEN MOVE J+1 TO NDIAG-1 DOWN +C PUT I AT TOP(J) +C DIAGO FINDS THE INTERSECTION OF DIAGONAL I ON THE 2 AXES +C FIRST FIND THE TOP SCORES AND THERE HIST ELEMENT NO +C THEN GET THEIR AXES INTERSECTIONS + IDIAG = 0 + CALL FILLI(TOPD,NDIAG,0) + CALL FILLI(TOPI,NDIAG,0) + CALL FILLI(TOPJ,NDIAG,0) + RMSQ = 0. + RM = 0. + DO 20 I = -IDIM1,IDIM2 + RJ = HIST(I) + RM = RM + RJ + RMSQ = RMSQ + RJ * RJ +20 CONTINUE + N = IDIM1 + IDIM2 + RM = RM / N + RMSQ = RMSQ / N + RM2 = RM * RM + SD = 0. + T = RMSQ - RM2 + IF(T.GT.0.) SD = SQRT(T) + MINS = NINT(RM + RNSD * SD) +C TRY TO FIND HIGHEST SCORES FIRST + DO 100 I = 0,IDIM2 + M = HIST(I) + IF(M.GT.MINS) THEN + IDIAG = IDIAG + 1 + IF(M.GT.TOPD(NDIAG)) THEN + DO 50 J = 1,NDIAG + IF(M.GT.TOPD(J)) THEN + DO 40 K = NDIAG-1,J,-1 + TOPD(K+1) = TOPD(K) + TOPI(K+1) = TOPI(K) +40 CONTINUE + TOPD(J) = M + TOPI(J) = I + GO TO 60 + END IF +50 CONTINUE + END IF + END IF +60 CONTINUE +100 CONTINUE + DO 200 I = -1,-IDIM1,-1 + M = HIST(I) + IF(M.GT.MINS) THEN + IDIAG = IDIAG + 1 + IF(M.GT.TOPD(NDIAG)) THEN + DO 150 J = 1,NDIAG + IF(M.GT.TOPD(J)) THEN + DO 140 K = NDIAG-1,J,-1 + TOPD(K+1) = TOPD(K) + TOPI(K+1) = TOPI(K) +140 CONTINUE + TOPD(J) = M + TOPI(J) = I + GO TO 160 + END IF +150 CONTINUE + END IF + END IF +160 CONTINUE +200 CONTINUE + NDIAG = MIN(IDIAG,NDIAG) + DO 300 I = 1,NDIAG + J = TOPI(I) + CALL DIAGO(J,TOPI(I),TOPJ(I)) +300 CONTINUE + END + SUBROUTINE DIAGO(I,II,JJ) + IF(I.GE.0) THEN + JJ = 1 + II = I + 1 + ELSE + II = 1 + JJ = ABS(I) + 1 + END IF + END + SUBROUTINE WDSCR(SEQ1N,SEQS,IDIM1,KTUP,MAIND,IDM) + INTEGER SEQ1N(IDIM1),SEQS(IDIM1),MAIND(IDM) + DO 10 I =1,IDIM1-KTUP+1 + K = 0 + DO 5 J = I,I+KTUP-1 + K = K + MAIND(SEQ1N(J)) +5 CONTINUE + SEQS(I) = K +10 CONTINUE + END + SUBROUTINE DP21(MATMAX,AVSCOR,LENGTH,MINPRO, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER HELPF*(*) + MN = 1 + MX = MAX(1,MATMAX-1) * LENGTH + MINPRO = INT(AVSCOR*LENGTH) + CALL GETINT(MN,MX,MINPRO,'Proportional score', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.EQ.0) MINPRO = IVAL + END + SUBROUTINE DP22(IDM,MINPER, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER HELPF*(*) + MN = 1 + IF(IDM.EQ.5) THEN + MX = 100 + ELSE + MX = 20 + END IF + CALL GETINT(MN,MX,MINPER,'Identity score', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.EQ.0) MINPER = IVAL + END + SUBROUTINE DP20(LENGTH,MXSPAN,IDIMVA,IDIMHA,IDIMVP,IDIMHP, + +NOROO,NOROE,MAXSEQ, + +SEQVC,IDIMBV,ISV1,ISV2,SEQV,MXCOMP, + +CHRSET,IDM,MSPO2,ISTARV,IENDV, + +SEQHC,IDIMBH,ISH1,ISH2,SEQH, + +ISTARH,IENDH,LB,LF, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER HELPF*(*),NOROO,NOROE,CHRSET(IDM) + CHARACTER SEQHC(MAXSEQ),SEQVC(MAXSEQ) + INTEGER SEQH(MXCOMP),SEQV(MXCOMP) + LTEMP=LENGTH +5 CONTINUE + MN = 1 + MX = MIN(MXSPAN,MIN(IDIMVA,IDIMHA)) + CALL GETINT(MN,MX,LENGTH,'Odd span length', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF(MOD(IVAL,2).NE.1)GO TO 5 + LENGTH = IVAL + LB=(LENGTH+1)/2 + LF=LENGTH/2 + IDIMVP=IDIMVA+LENGTH + IDIMHP=IDIMHA+LENGTH + NOROO='N' + NOROE='N' +C IF LENGTH LONGER THAN BEFORE EXTEND INTEGER BUFFERS + IF(LTEMP.LT.LENGTH)THEN + CALL FILEDG(SEQVC,IDIMBV,ISV1,ISV2,SEQV,MXCOMP,LENGTH, + + CHRSET,IDM,MSPO2,ISTARV,IENDV) + CALL FILEDG(SEQHC,IDIMBH,ISH1,ISH2,SEQH,MXCOMP,LENGTH, + + CHRSET,IDM,MSPO2,ISTARH,IENDH) + END IF + END + SUBROUTINE DP33(RNSD, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER HELPF*(*) + RMN = 0. + RMX = 10. + CALL GETRL(RMN,RMX,RNSD,'Number of sd above mean', + + RVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.EQ.0) RNSD = RVAL + END + SUBROUTINE CFSQL(SEQ1,IDIM1,SEQ2,IDIM2,POSN,WORDP,IDE,IDCHAR, + +CONSTS,LCONST,LENGTH,MINMAT,IDEV,KBOUT,ISAME,SEQ,HIST,MAXSEQ) + INTEGER SEQ1(IDIM1),SEQ2(IDIM2),HIST(-MAXSEQ:MAXSEQ) + INTEGER POSN(IDIM1),WORDP(IDE),CONSTS(0:LCONST) + CHARACTER SEQ(IDIM1) + CALL BUSY(KBOUT) + CALL SETCN(CONSTS,LENGTH,IDCHAR,LCONST) + CALL ENCONC(SEQ1,IDIM1-MINMAT+1,POSN,WORDP,IDE,IDCHAR, + +CONSTS,LENGTH,LCONST) + CALL CFSEQL(SEQ1,IDIM1,POSN,WORDP,IDE,SEQ2,IDIM2,CONSTS,LCONST, + +LENGTH,IDCHAR,MINMAT,IDEV,ISAME,SEQ,HIST,MAXSEQ) + END + SUBROUTINE CFSEQL(SEQ1,IDIM1,POSN,WORDP,IDE,SEQ2,IDIM2,CONSTS, + +LCONST, + +LENGTH,IDCHAR,MINMAT,IDEV,ISAME,SEQ,HIST,MAXSEQ) + INTEGER SEQ1(IDIM1),SEQ2(IDIM2) + INTEGER POSN(IDIM1),WORDP(IDE),CONSTS(0:LCONST) + INTEGER NCODEA,EXTNDN + INTEGER HIST(-MAXSEQ:MAXSEQ) + CHARACTER SEQ(IDIM1) + EXTERNAL NCODEA,EXTNDN + LEX = MINMAT - LENGTH - 1 +C +C if we are looking for internal repeats we switch off half the matrix +C by making the saved score high +C + IF (ISAME.EQ.1) THEN + CALL FILLI(HIST(-IDIM1),IDIM1,IDIM1+IDIM2) + CALL FILLI(HIST(0),IDIM2,0) + ELSE + CALL FILLI(HIST(-IDIM1),IDIM1+IDIM2+1,0) + END IF + DO 20 I = 1,IDIM2-MINMAT+1 + J = NCODEA(SEQ2(I),LENGTH,CONSTS,IDCHAR,LCONST) + IF(J.NE.0)THEN + J1 = WORDP(J) + IF(J1.NE.0)THEN + IF (((ISAME.EQ.1).AND.(I.NE.J1)).OR.(ISAME.EQ.0)) THEN + LT = 1 + MIN(IDIM1-(J1+LENGTH),IDIM2-(I+LENGTH)) + IMATCH = EXTNDN(SEQ1(J1+LENGTH),SEQ2(I+LENGTH),LT) + IF(IMATCH.GT.LEX)THEN + L = I + IMATCH + LENGTH + K = I - J1 +C +C if this match ends furthest away we display it and save it +C + IF (HIST(K).LT.L) THEN + HIST(K) = L + WRITE(IDEV,1000)I,J1,IMATCH+LENGTH + 1000 FORMAT(' Positions',I7,'h',I7,'v and length',I7) + WRITE(IDEV,1001)(SEQ(K),K=J1,J1+IMATCH+LENGTH-1) + 1001 FORMAT(' ',50A1) + END IF + END IF + END IF +10 CONTINUE + J2 = J1 + J1 = POSN(J2) + IF(J1.NE.0)THEN + IF (((ISAME.EQ.1).AND.(I.NE.J1)).OR.(ISAME.EQ.0)) THEN + LT = 1 + MIN(IDIM1-(J1+LENGTH),IDIM2-(I+LENGTH)) + IMATCH = EXTNDN(SEQ1(J1+LENGTH),SEQ2(I+LENGTH),LT) + IF(IMATCH.GT.LEX)THEN + L = I + IMATCH + LENGTH + K = I - J1 + IF (HIST(K).LT.L) THEN + HIST(K) = L + WRITE(IDEV,1000)I,J1,IMATCH+LENGTH + WRITE(IDEV,1001)(SEQ(K),K=J1,J1+IMATCH+LENGTH-1) + END IF + END IF + END IF + GO TO 10 + END IF + END IF + END IF +20 CONTINUE + END + INTEGER FUNCTION EXTNDN(SEQ1,SEQ2,LENGTH) + INTEGER SEQ1(LENGTH),SEQ2(LENGTH) + DO 10 I = 1,LENGTH + IF(SEQ1(I).NE.SEQ2(I)) THEN + EXTNDN = I - 1 + RETURN + END IF +10 CONTINUE + EXTNDN = LENGTH + END diff --git a/src/staden/edMenu.c b/src/staden/edMenu.c new file mode 100644 index 0000000..aef3084 --- /dev/null +++ b/src/staden/edMenu.c @@ -0,0 +1,140 @@ +/* +** Menu driver for xdap contig editor +** +** +** To add new menu entries: +** 1. Add a new option name to menuOptions. +** 2. Create a new ed_menu_option line, providing the string to appear on +** menu, and menu option. +** 3. Add a call to your interface routine to the switch statement in +** MenuSelectCallBack. +** 4. That's all +*/ + +#include +#include + +#include +#include +#include + +#include "edMenu.h" +#include "edUtils.h" +#include "contigEditor.h" + +/* ---- Types ---- */ + +typedef struct +{ String name; /* Name of function */ + int number; /* Number of function */ +} MenuData, *MenuDataList; + + +enum menuOptions { + OptLine, + OptSearchGeneric, + OptSaveContig, + OptCreateTag, + OptEditTag, + OptDeleteTag, + OptShowDifferences, + OptDumpContig + }; + +static MenuData ed_menu_options[] = +{ + {"Search", OptSearchGeneric}, + {"Highlight Disagreements", OptShowDifferences}, + {"", OptLine}, + {"Save Contig", OptSaveContig}, + {"Dump Contig to File", OptDumpContig}, + {"", OptLine}, + {"Create Tag", OptCreateTag}, + {"Edit Tag", OptEditTag}, + {"Delete Tag", OptDeleteTag}, +}; + + + + + + + + + +static void MenuSelectCallback(Widget w, XtPointer i, XtPointer junk) +{ + EdStruct *xx = widgetToEdStruct(XtParent(XtParent(XtParent(w)))); + + switch ( (int) i ) { + case OptSaveContig: + saveDB( + xx, + saveState.idevr, + saveState.idevw, + saveState.idevn, + saveState.relpg, + saveState.lngthg, + saveState.lnbr, + saveState.rnbr, + saveState.maxgel + ); + break; + case OptCreateTag: + createTag(xx); + break; + case OptEditTag: + editTag(xx); + break; + case OptDeleteTag: + deleteTag(xx); + break; + case OptSearchGeneric: + invokeSearchGeneric(xx); + break; + case OptShowDifferences: + xx->showDifferences ^= 1; + redisplaySequences (xx,xx->namesWid, xx->sequencesWid, xx->displayPos, xx->displayWidth); + break; + case OptDumpContig: + dumpContig(xx); + break; + default: + break; + } +} + + +Widget createEdMenu(Widget parentWid) +{ + Widget menuWid; + int i; + + Cardinal num_md = XtNumber(ed_menu_options); + /* + Create the menu parent widget + */ + menuWid = XtCreatePopupShell("edMenu", simpleMenuWidgetClass, parentWid, + NULL, 0); + + /* + Put the individual items in. + When selected, each entry will generate a callback with + its associated number. + */ + for (i = 0; i < (int) num_md ; i++) + { + if (ed_menu_options[i].number == OptLine) { + (void) XtCreateManagedWidget("line", smeLineObjectClass, menuWid, NULL, (Cardinal)0); + } else { + Widget entryWid = XtCreateManagedWidget(ed_menu_options[i].name, smeBSBObjectClass, + menuWid, NULL, 0); + XtAddCallback(entryWid, XtNcallback, MenuSelectCallback, + (XtPointer) ed_menu_options[i].number); + } + } + + return menuWid; + +} + diff --git a/src/staden/edMenu.h b/src/staden/edMenu.h new file mode 100644 index 0000000..4aceab1 --- /dev/null +++ b/src/staden/edMenu.h @@ -0,0 +1,10 @@ +#ifndef _edMenu_h +#define _edMenu_h + +#include + +/* ---- Exported Functions ---- */ + +extern Widget createEdMenu(Widget parentWid); + +#endif /* _edMenu_h */ diff --git a/src/staden/edUtils.c b/src/staden/edUtils.c new file mode 100644 index 0000000..61690c1 --- /dev/null +++ b/src/staden/edUtils.c @@ -0,0 +1,2285 @@ +/* + Title: edUtils + + File: edUtils.c + Purpose: C language utilities for contig editor + Last update: 5 April 1993 +*/ + + +/* + 9/7/91 SD Added Trace Title field to call manageTrace + 26/7/91 SD Tightened up editing options: + 1. Allow only insertion and deletion of asterisks from consensus + This means no replacement. This CANNOT be overridden + 2. Do not allow edits when cursor is not visible on the screen + 28/7/91 SD Use FILE_NAME_LENGTH for trace file name length in showTrace + 1/10/91 SD Removed static from calculateConsensusLength + Removed LLINOL and LLINOR from call to dojoin_ + + 28/4/92 SD General Speedup + 20/8/92 SD save state variable for returning what happened during edit/join + 01/03/93 SD New dump contig command + 05/04/93 JKB Check for editorState (disable edits after 'Leave Editor'). + +*/ + + +#define CHKPNT(W,S) \ + { XSync(XtDisplay(W),False); fprintf(stderr,"CHKPNT: %s\n",S); } + + +/* ---- Includes ---- */ + +#include +#include +#include +#include +#include +#include +#include +#include /* IMPORT: XBell */ +#include "Sheet.h" + +#include "edUtils.h" +#include "contigEditor.h" +#include "tman_main.h" +#include "main.h" +#include "select.h" +#include "tagUtils.h" +#include "undo.h" +#include "extend.h" +#include "fortran.h" +#include "fort.h" +#include "misc.h" + + +/* ------ External variables ------- */ +extern int_f save_state; +extern enum States {StateDown=0,StateUp} editorState; + +/* +** Static variables +** defining the state of the contig editor +*/ +#define MAXEDSTATES 2 +static int EdStructCount = 0; +static EdStruct edstate[MAXEDSTATES] = { + { + /* oldMax */ 0, + /* DB_flags */ DB_ACCESS_UPDATE | + DB_DELAYED_READ | + DB_DATA_TYPE_DNA, + /* DB_gelCount */ 0, + /* DB_contigNum */ 0, + /* DBlist */ NULL, + /* DBorder */ NULL, + /* DB */ NULL, + /* displayPos */ 1, + /* displayWidth */ DEFAULT_DISPLAY_WIDTH, + /* displayHeight */ 0, + /* cursorPos */ 1, + /* cursorSeq */ 0, + /* rulerDisplayed */ 1, + /* consensusDisplayed */ 1, + /* fontWidth */ 0, + /* fontHeight */ 0, + /* edWid */ NULL, + /* namesWid */ NULL, + /* sequencesWid */ NULL, + /* scrollButtonsWid */ NULL, + /* sliderWid */ NULL, + /* displayedConsensus */ "", + /* select_made */ 0, + /* select_seq */ 0, + /* select_start_pos */ 0, + /* select_end_pos */ 0, + /* select_tag */ NULL, + /* reveal_cutoffs */ 0, + /* showDifferences */ 0 + }, + { + /* oldMax */ 0, + /* DB_flags */ DB_ACCESS_UPDATE | + DB_DELAYED_READ | + DB_DATA_TYPE_DNA, + /* DB_gelCount */ 0, + /* DB_contigNum */ 0, + /* DBlist */ NULL, + /* DBorder */ NULL, + /* DB */ NULL, + /* displayPos */ 1, + /* displayWidth */ DEFAULT_DISPLAY_WIDTH, + /* displayHeight */ 0, + /* cursorPos */ 1, + /* cursorSeq */ 0, + /* rulerDisplayed */ 1, + /* consensusDisplayed */ 1, + /* fontWidth */ 0, + /* fontHeight */ 0, + /* edWid */ NULL, + /* namesWid */ NULL, + /* sequencesWid */ NULL, + /* scrollButtonsWid */ NULL, + /* sliderWid */ NULL, + /* displayedConsensus */ "", + /* select_made */ 0, + /* select_seq */ 0, + /* select_start_pos */ 0, + /* select_end_pos */ 0, + /* select_tag */ NULL, + /* reveal_cutoffs */ 0, + /* showDifferences */ 0 + } +}; + +static void bell() +/* +** Make a silly beep +*/ +{ + XBell (GetDisplay(),100); +} + + + +void calculateConsensusLength(EdStruct *xx) +/* +** Calculate dynamic consensus length +*/ +{ + int sequenceEnd,i; + + DBsetLength(xx,0,0); + for (i=1; i <= xx->DB_gelCount; i++) { + sequenceEnd = DBgetRelPos(xx,i) + DBgetLength(xx,i) -1; + if (DBgetLength(xx,0) < sequenceEnd) DBsetLength(xx,0,sequenceEnd); + } +} + +EdStructPtr getFreeEdStruct() +/* +** Get the next free EdStruct +*/ +{ + if (EdStructCount == MAXEDSTATES) + return NULL; + else + return &edstate[EdStructCount++]; +} + +EdStructPtr intToEdStruct(int i) +/* +** Given an integer, +** return the corresponding edit structure +*/ +{ + return &edstate[i]; +} + +EdStructPtr widgetToEdStruct(Widget w) +/* +** Given a widget, +** return the corresponding edit structure +*/ +{ + int i; + if (w==NULL) + return NULL; + else + for (i=0;ishowDifferences = 0; + + /* + ** determine gel number + */ + { + int dummy,numContigs; + int firstGel; + + readr_(idevr,idbsiz,&dummy,&numContigs,&dummy,&dummy); + xx->DB_contigNum = (int)*idbsiz-numContigs; + readr_(idevr,&xx->DB_contigNum,&dummy,&dummy,&firstGel,&dummy); + while (xx->DB_contigNum < (int)*idbsiz && firstGel!=(int)*llino) { + xx->DB_contigNum++; + readr_(idevr,&xx->DB_contigNum,&dummy,&dummy,&firstGel,&dummy); + } + + } + + /* + ** count number of gels in contig + */ + for (xx->DB_gelCount=1,i=(int) *llino; + xx->DB_gelCount<(int)*idbsiz && (int)rnbr[i-1]; + xx->DB_gelCount++,i=(int)rnbr[i-1]); + + if ((xx->DB = (DBStruct *) calloc (xx->DB_gelCount+1, sizeof(DBStruct)))==NULL) + goto disaster; + if ((xx->DBlist = (int *) calloc (xx->DB_gelCount+1, sizeof(int) ))==NULL) + goto disaster; + if ((xx->DBorder = (int *) calloc (xx->DB_gelCount+1, sizeof(int) ))==NULL) + goto disaster; + + /* + ** read information into local database + */ + for (c=1,i=(int) *llino; + c<(int)*idbsiz && i; + c++,i=(int)rnbr[i-1]) { + + DBsetRelPos(xx,c,relpg[i-1]); + DBsetLength(xx,c,abs(lngthg[i-1])); + DBsetComp(xx,c,(lngthg[i-1]<0)?-1:1); + DBsetNumber(xx,c,i); + DBsetFlags(xx,c,DB_FLAG_NONE); + + xx->DBorder[c] = c; + + if (xx->DB_flags & DB_STORAGE_INTERNAL) { + if (DBgetSeq(xx,c)==NULL) goto disaster; + if (DBgetName(xx,c)==NULL) goto disaster; + (void)DBgetTags(xx,c); + } + + } + /* + ** Set up consensus + */ + { + DBsetRelPos(xx,0,1); + DBsetComp(xx,0,UNCOMPLEMENTED); + calculateConsensusLength(xx); + if ((DBsetSeq(xx,0,(char *) malloc(MAX_DISPLAY_WIDTH)))==NULL) + goto disaster; + if ((DBsetName(xx,0,(char *) malloc(sizeof(char)*(NAMELEN+1))))==NULL) + goto disaster; + sprintf(DB_Name(xx,0),"%*s %-*s", + DB_GELNOLEN," ", + DB_NAMELEN, "CONSENSUS"); + xx->DBorder[0] = 0; + } + + return 0; + +disaster: + + freeDB(xx); + return 1; +} + +void freeDB(EdStruct *xx) +/* +** Free an internal database +*/ +{ + int i; + + if (xx->DB != NULL) + for (i=0; i <= xx->DB_gelCount; i++) { + free(DB_Name(xx,i)); + free(DB_Seq(xx,i)); + destroyTagList(DB_Tags(xx,i)); + } + + free(xx->DB); + free(xx->DBlist); + free(xx->DBorder); + + xx->DB = NULL; + xx->DBlist = NULL; + xx->DBorder = NULL; +} + + +void saveDB( + EdStruct *xx, + int_f *idevr, /* unit number for relationships */ + int_f *idevw, /* unit number for working versions of sequences */ + int_f *idevn, /* unit number for sequence names */ + int_f *relpg, /* relative positions of gels in sequences */ + int_f *lngthg, /* lengths of sequences */ + int_f *lnbr, /* left neighbours */ + int_f *rnbr, /* right neighbours */ + int_f *maxgel /* maximum length of gel */ + ) +/* +** Save an internal database +*/ +{ + int i; + int N,leftN,rightN; + int flag; + + for (i=1; i<=xx->DB_gelCount; i++) { + /* + ** update relationships + */ + N = DBgetNumber(xx,xx->DBorder[i]); + relpg[N-1] = DBgetRelPos(xx,xx->DBorder[i]); + lngthg[N-1] = (DBgetComp(xx,xx->DBorder[i])==COMPLEMENTED)?-DBgetLength(xx,xx->DBorder[i]):DBgetLength(xx,xx->DBorder[i]); + if (i==1) + leftN = 0; + else + leftN = DBgetNumber(xx,xx->DBorder[i-1]); + if (i==xx->DB_gelCount) + rightN = 0; + else + rightN = DBgetNumber(xx,xx->DBorder[i+1]); + lnbr[N-1] = leftN; + rnbr[N-1] = rightN; + writer_(idevr,&N,&relpg[N-1],&lngthg[N-1],&lnbr[N-1],&rnbr[N-1]); + + flag = DBgetFlags(xx,xx->DBorder[i]); + /* + ** update working versions + */ + if (flag&(DB_FLAG_SEQ_IN_MEMORY|DB_FLAG_SEQ_MODIFIED)) + writew_(idevw,&N,DB_Seq(xx,xx->DBorder[i]),maxgel,*maxgel); + /* + ** update tag list + */ + if (flag&(DB_FLAG_TAG_IN_MEMORY|DB_FLAG_TAG_MODIFIED)) { + writeTagList(xx, xx->DBorder[i]); + destroyTagList(DB_Tags(xx,xx->DBorder[i])); + DBsetTags(xx,xx->DBorder[i],readTagList(DBgetNumber(xx,xx->DBorder[i]))); + } + + /* + ** Preserve only ``sequence in memory flag'' + */ + DBsetFlags(xx,xx->DBorder[i], + flag&(DB_FLAG_SEQ_IN_MEMORY || + DB_FLAG_NAME_IN_MEMORY || + DB_FLAG_TAG_IN_MEMORY)); + + } + + /* + ** update contig relationships + */ + calculateConsensusLength(xx); + relpg[xx->DB_contigNum-1] = DBgetLength(xx,0); + lngthg[xx->DB_contigNum-1] = 0; + lnbr[xx->DB_contigNum-1] = DBgetNumber(xx,xx->DBorder[1]); + rnbr[xx->DB_contigNum-1] = DBgetNumber(xx,xx->DBorder[xx->DB_gelCount]); + writer_(idevr, + &xx->DB_contigNum, + &relpg[xx->DB_contigNum-1], + &lngthg[xx->DB_contigNum-1], + &lnbr[xx->DB_contigNum-1], + &rnbr[xx->DB_contigNum-1] + ); + flushl_(idevr); + flushl_(idevw); + flushl_(idevn); /* not needed really */ + flushl_(&devils_.idevt); + flushl_(&devils_.idevc); + + cleanUpStack(&bucketStack); + + /* update save state */ + if(xx == intToEdStruct(0)) + save_state |= (int_f)1; + else + save_state |= (int_f)2; + + +} + +void joinDB( + int_f *idevr, /* unit number for relationships */ + int_f *idevw, /* unit number for working versions of sequences */ + int_f *idevn, /* unit number for sequence names */ + int_f *relpg, /* relative positions of gels in sequences */ + int_f *lngthg, /* lengths of sequences */ + int_f *lnbr, /* left neighbours */ + int_f *rnbr, /* right neighbours */ + int_f *maxgel, /* maximum length of gel */ + int_f *llinol, + int_f *lnconl, + int_f *llinor, + int_f *lnconr, + int_f *ngels, + int_f *nconts, + int_f *idbsiz + ) +{ + int_f relx; + int i; + + /* + ** Save an internal databases + */ + for (i=0;i<2;i++) + saveDB( + intToEdStruct(i), + idevr, + idevw, + idevn, + relpg, + lngthg, + lnbr, + rnbr, + maxgel + ); + relx = editorLockedPos(1/*force*/); + if (relx<0) { + relx = 1-relx; + dojoin_(relpg,lngthg,lnbr,rnbr,ngels,nconts,lnconl,lnconr, + idbsiz,idevr,idevw,&relx); + } else { + relx = 1+relx; + dojoin_(relpg,lngthg,lnbr,rnbr,ngels,nconts,lnconr,lnconl, + idbsiz,idevr,idevw,&relx); + } + + save_state |= (int_f)4; + +} + + +static int linesInRegion(EdStruct *xx, int pos, int width) +/* +** Return number of sequences on screen +*/ +{ + int i, count; + + for (i=1,count=0 ; + i<=xx->DB_gelCount && DBgetRelPos(xx,xx->DBorder[i])<(pos+width) ; + i++) { + if (DBgetRelPos(xx,xx->DBorder[i])+DBgetLength(xx,xx->DBorder[i])>pos && + DBgetLength(xx,xx->DBorder[i])) count++; + } + count += xx->consensusDisplayed; + + return count; + +} + + +static int linesOnScreen (EdStruct *xx, int pos, int width) +/* +** Return number of sequences on screen +*/ +{ + int i, count; + + for (i=1,count=0 ; i<=xx->DB_gelCount ; i++) { + int relPos, length; + int len_lcut, len_rcut; + + if (xx->reveal_cutoffs) { + len_lcut = lenLCut(xx,xx->DBorder[i]); + len_rcut = lenRCut(xx,xx->DBorder[i]); + } else + len_lcut = len_rcut = 0; + relPos = DBgetRelPos(xx,xx->DBorder[i]) - len_lcut; + length = DBgetLength(xx,xx->DBorder[i]) + len_lcut + len_rcut; + if (relPos < pos+width && relPos+length>pos) + count++; + } + count += xx->consensusDisplayed; + + return count; +} + + + +#ifdef notdef +static int maxLinesOnScreen_x (EdStruct *xx, int width) +/* +** Given width, return maximum number of lines on screen +*/ +{ + int i,max,linesAtX,X; + + max = linesOnScreen(xx,1,width); + for (i=1 ; i <= xx->DB_gelCount ; i++) { + X=DBgetRelPos(xx,i)-width+1; + if (X<=1) continue; + linesAtX=linesOnScreen(xx,X,width); + if (linesAtX>max) max = linesAtX; + } + max += xx->consensusDisplayed; + + return max; +} +#endif + +int *sequencesInRegion(EdStruct *xx,int pos, int width) +/* +** Return a pointer to list of sequences in region of contig +*/ +{ + int i, count; + + for (i=1,count=0 ; i<=xx->DB_gelCount && DBgetRelPos(xx,xx->DBorder[i])<(pos+width) ; i++) { + if (DBgetRelPos(xx,xx->DBorder[i])+DBgetLength(xx,xx->DBorder[i])>pos && DBgetLength(xx,xx->DBorder[i])) + xx->DBlist[count++]=xx->DBorder[i]; + } + if (xx->consensusDisplayed) xx->DBlist[count++] = 0; + + return xx->DBlist; + +} + + +int *sequencesOnScreen(EdStruct *xx,int pos, int width) +/* +** Return a pointer to list of sequences on screen +*/ +{ + int i, count; + + for (i=1,count=0 ; i<=xx->DB_gelCount ; i++) { + int relPos, length; + int len_lcut, len_rcut; + + if (xx->reveal_cutoffs) { + len_lcut = lenLCut(xx,xx->DBorder[i]); + len_rcut = lenRCut(xx,xx->DBorder[i]); + } else + len_lcut = len_rcut = 0; + relPos = DBgetRelPos(xx,xx->DBorder[i]) - len_lcut; + length = DBgetLength(xx,xx->DBorder[i]) + len_lcut + len_rcut; + if (relPos < pos+width && relPos+length>pos) + xx->DBlist[count++]=xx->DBorder[i]; + } + if (xx->consensusDisplayed) xx->DBlist[count++] = 0; + + return xx->DBlist; + +} + +void DBgetSequence(EdStruct *xx, int seq, int pos, int width, char *str) +/* +** get part of a sequence from its `pos' base for `width' bases +** Bases number from 0? +*/ +{ + char *src; + int length = DBgetLength(xx,seq); + int i; + + src = DBgetSeq(xx,seq); + + /* Lefthand cut off */ + if (pos<0) { + i = (width<-pos)?width:-pos; + getLCut(xx,seq, -pos, i, str); + } else + i=0; + + /*copy sequence*/ + for (; + iDB_flags & DB_DATA_TYPE) { + ** case DB_DATA_TYPE_DNA: do dna stuff + ** case DB_DATA_TYPE_PROTEIN: do protein stuff + ** default : do dna stuff??? + ** } + ** BASES = 6 for dna, 26 for protein (specified by fortran variable IDM?) + */ + int_f scores[MAX_DISPLAY_WIDTH][BASES]; + int_f index,score; + int_f i,j,k; + int_f seven=BASES; + int jlow,klow,jhigh /* ,khigh */; + int chunk; /* do in chucks of MAX_DISPLAY_WIDTH */ + int cwidth; + + for (chunk=0; chunkMAX_DISPLAY_WIDTH) cwidth=MAX_DISPLAY_WIDTH; + /*now do for pos+chunk to pos+chunk+cwidth*/ + + for (i=0;iDB_gelCount && + DBgetRelPos(xx,xx->DBorder[i])<(pos+chunk+cwidth) ; + i++) + { + if (DBgetRelPos(xx,xx->DBorder[i]) + + DBgetLength(xx,xx->DBorder[i]) > (pos+chunk) && + DBgetLength(xx,xx->DBorder[i]) && + (mode == BOTH_STRANDS || + mode == DBgetComp(xx,xx->DBorder[i]))) + { + if (DBgetRelPos(xx,xx->DBorder[i]) < (pos+chunk)) + { + klow = (pos+chunk) - DBgetRelPos(xx,xx->DBorder[i]); + jlow = 0; + } + else + { + klow = 0; + jlow = DBgetRelPos(xx,xx->DBorder[i]) - (pos+chunk); + } + + if (DBgetRelPos(xx,xx->DBorder[i]) + + DBgetLength(xx,xx->DBorder[i]) > pos+chunk+cwidth) + { + /*khigh = klow + cwidth;*/ + jhigh = cwidth; + } + else + { + /*khigh = DBgetLength(xx,xx->DBorder[i]);*/ + jhigh = jlow + DBgetLength(xx,xx->DBorder[i]) - klow; + } + + { + char s[MAX_DISPLAY_WIDTH+1]; + DBgetSequence(xx,xx->DBorder[i],klow,jhigh-jlow,s); + for (j=jlow,k=0; j= xx->displayPos && + posInContig < xx->displayPos + xx->displayWidth ); +} + + +#ifdef notdef +static void getJoinedData(EdStruct *xx, int *leftPos, int* rightPos) +/* +** get information about relative positions of two joined contigs +*/ +{ + int offset = editorLockedPos(0/*don't force recalculation*/); + EdStruct *otherxx; + + otherxx = intToEdStruct(0); + if (otherxx==xx) { + otherxx = intToEdStruct(1); + *leftPos = min(1,1-offset); + *rightPos = max(DBgetLength(xx,0),DBgetLength(otherxx,0)-offset); + } else { + *leftPos = min(1,1+offset); + *rightPos = max(DBgetLength(xx,0),DBgetLength(otherxx,0)+offset); + } +} +#endif /*notdef*/ + +#ifdef notdef +static int getJoinedLength(EdStruct *xx) +/* +** Get the combined length of the two joined contigs +*/ +{ + int leftPos, rightPos; + getJoinedData(xx,&leftPos,&rightPos); + return rightPos - leftPos; +} + +static int getJoinedLeftPos(EdStruct *xx) +/* +** Get the position (relative to xx) of the leftmost base in +** the joined contig +*/ +{ + int leftPos, rightPos; + getJoinedData(xx,&leftPos,&rightPos); + return leftPos; +} + +static int getJoinedRightPos(EdStruct *xx) +/* +** Get the position (relative to xx) of the rightmost base in +** the joined contig +*/ +{ + int leftPos, rightPos; + getJoinedData(xx,&leftPos,&rightPos); + return rightPos; +} +#endif + +static void extents(EdStruct *xx, int *left, int *right) +/* +** Get maximum extents of sequence, taking into account cutoffs. +*/ +{ + + if (xx->reveal_cutoffs) { + int eleft, eright; + int i; + + eleft = eright = 0; + + for (i=1; i<=xx->DB_gelCount ; i++) { + int thisleft,thisright; + + thisleft = DBgetRelPos(xx,xx->DBorder[i]) - lenLCut(xx,xx->DBorder[i]); + thisright = DBgetRelPos(xx,xx->DBorder[i]) + DBgetLength(xx,xx->DBorder[i]) + lenRCut(xx,xx->DBorder[i]) - 1; + + if (eleft > thisleft) + eleft = thisleft; + if (eright < thisright) + eright = thisright; + } + + *left = eleft; + *right = eright; + + } else { + *left = 1; + *right = DBgetLength(xx,0); + } + +} + + + + +static void joinedExtents(EdStruct *xx, int *leftPos, int* rightPos) +/* +** get information about relative positions of two joined contigs +*/ +{ + int offset = editorLockedPos(0/*don't force recalculation*/); + EdStruct *otherxx; + int left,right; + int otherleft,otherright; + + otherxx = intToEdStruct(0); + extents(xx,&left,&right); + if (otherxx==xx) { + otherxx = intToEdStruct(1); + extents(otherxx,&otherleft,&otherright); + *leftPos = min(left,otherleft-offset); + *rightPos = max(right,otherright-offset); + } else { + extents(otherxx,&otherleft,&otherright); + *leftPos = min(left,otherleft+offset); + *rightPos = max(right,otherright+offset); + } +} + + + + + + +static void getExtents(EdStruct *xx, int *left, int *right) +{ + if (inJoinMode() && editorLocked()) + joinedExtents(xx,left,right); + else { + extents(xx,left,right); + if (inJoinMode() && !editorLocked()) { + *right += xx->displayWidth - 2; + *left -= xx->displayWidth - 1; + } + } + + +} + + + +/***************************************************/ +/**/ +/**/ + + + + + +static void setSliderPos (EdStruct *xx, int pos) +/* +** set slider as a position in contig +*/ +{ + float percentSet; + float percentShown; + int left, right; + getExtents(xx,&left,&right); + percentSet = (float)(pos-left)/(float)(right-left+1+1-xx->displayWidth); + percentShown = (float)xx->displayWidth/(float)(right-left+1+1); + + XawScrollbarSetThumb(xx->sliderWid,percentSet,percentShown); +} + + + + + + +static void incDisplayPosP (EdStruct *xx, int distance) +/* +** Increase the leftmost base position on the screen by a symbolic amount +*/ +{ + int oldPos = xx->displayPos; + int left,right; + switch (distance) { + case D_screen : xx->displayPos += xx->displayWidth; break; + case D_halfScreen : xx->displayPos += xx->displayWidth/2; break; + case D_character : xx->displayPos += 1; break; + } + getExtents(xx,&left,&right); + if (xx->displayPos > right + 2 - xx->displayWidth) + xx->displayPos = right + 2 - xx->displayWidth; + if (oldPos!=xx->displayPos) + redisplaySequences (xx,xx->namesWid, xx->sequencesWid, xx->displayPos, xx->displayWidth); +} + + + + + + + + +static void decDisplayPosP (EdStruct *xx, int distance) +/* +** Decrease the leftmost base position on the screen by a symbolic ammount +*/ +{ + int oldPos = xx->displayPos; + int left,right; + switch (distance) { + case D_screen : xx->displayPos -= xx->displayWidth; break; + case D_halfScreen : xx->displayPos -= xx->displayWidth/2; break; + case D_character : xx->displayPos -= 1; break; + } + getExtents(xx,&left,&right); + if (xx->displayPos < left) + xx->displayPos = left; + if (oldPos!=xx->displayPos) + redisplaySequences (xx,xx->namesWid, xx->sequencesWid, xx->displayPos, xx->displayWidth); +} + + + + + + + + + +static void setDisplayPosPercentP (EdStruct *xx, float percent) +/* +** Set the leftmost base position on the screen given a percentage +*/ +{ + int oldPos = xx->displayPos; + int left,right; + getExtents(xx,&left,&right); + xx->displayPos = (int)((float) (right-left+1+1-xx->displayWidth) * percent) + left; + if (oldPos!=xx->displayPos) + redisplaySequences (xx,xx->namesWid, xx->sequencesWid, xx->displayPos, xx->displayWidth); +} + + + + + + + + +static void positionCursor(EdStruct *xx, Widget w, int seq, int pos) +/* +** position the cursor int sequence seq at position pos +** and indicate cursor if on screen +*/ +{ + if (onScreen(xx,seq,pos)) { + int screenRow,screenColumn; + int *seqList; + + screenColumn = positionInContig(xx,seq,pos) - xx->displayPos; + seqList = sequencesOnScreen(xx,xx->displayPos, xx->displayWidth); + for(screenRow=0; + screenRowdisplayHeight && seqList[screenRow] != seq; + screenRow++); + XawSheetDisplayCursor(w,True); + XawSheetPositionCursor(w,screenColumn,screenRow+xx->rulerDisplayed); + } else + XawSheetDisplayCursor(w,False); +} + +static void redisplayDisagreement() +/* +** Recalculate and redisplay all disagreements +*/ +{ + char spare[MAX_DISPLAY_WIDTH]; + EdStruct *xx[2]; + int i; + + if (inJoinMode()) { + xx[0] = intToEdStruct(0); + xx[1] = intToEdStruct(1); + for (i=0;idisplayWidth;i++) + spare[i]=(xx[0]->displayedConsensus[i] == + xx[1]->displayedConsensus[i])?' ':'!'; + XawSheetPutText(disagreeWid,0,0,xx[0]->displayWidth,spare); + } +} + + +void redisplaySequences (EdStruct *xx, Widget namesWid, Widget sequencesWid, + int pos, int width) +/* +** Redisplay the whole sequence display +*/ +{ + Arg args[10]; + int nargs; + int *seqList; + + setSliderPos(xx,pos); + + /* + ** Set Up Text Window sizes + */ + xx->displayHeight = linesOnScreen(xx,pos,width); + seqList = sequencesOnScreen(xx,pos, width); + + /* + ** Update names list + */ + nargs = 0; + XtSetArg(args[nargs], XtNrows, xx->displayHeight+xx->rulerDisplayed); nargs++; + XtSetValues(namesWid, args, nargs); + { + int i; + XawSheetInk splodge[NAMELEN]; + for (i=0 ; i < NAMELEN ; i++) splodge[i].sh = sh_inverse; + for (i=0 ; i < xx->displayHeight ; i++ ) { + if (DBgetFlags(xx,seqList[i]) & DB_FLAG_SELECTED) + XawSheetPutJazzyText(namesWid,0,(i+xx->rulerDisplayed),NAMELEN,DBgetName(xx,seqList[i]),splodge); + else + XawSheetPutText(namesWid,0,(i+xx->rulerDisplayed),NAMELEN,DBgetName(xx,seqList[i])); + } + } + + /* + ** Update sequence list + */ + nargs = 0; + XtSetArg(args[nargs], XtNrows, xx->displayHeight+xx->rulerDisplayed); nargs++; + XtSetValues(sequencesWid, args, nargs); + { + int i; + char spare[MAX_DISPLAY_WIDTH]; + if (xx->rulerDisplayed) { + char *k; + int j,lower,times; + lower = (pos - pos%10); + times = width/10 + 2; + for (j=0,k=spare;jdisplayedConsensus,BOTH_STRANDS); + for (i=0 ; i < xx->displayHeight ; i++ ) { + char * ptr; + XawSheetInk splodge[MAX_DISPLAY_WIDTH]; + + if (seqList[i]==0){ + ptr = xx->displayedConsensus; + }else{ + DBgetSequence(xx,seqList[i],pos-DBgetRelPos(xx,seqList[i]),width,spare); + ptr = spare; + } + if (xx->showDifferences) { + int j; + for (j=0;jdisplayedConsensus[j]) + spare[j]='.'; + } + getTagSplodge(xx,seqList[i],pos-DBgetRelPos(xx,seqList[i]),width,splodge); + XawSheetPutJazzyText(sequencesWid,0,(i+xx->rulerDisplayed),width,ptr,splodge); + } + + } + + positionCursor(xx,sequencesWid,xx->cursorSeq,xx->cursorPos); + redisplaySelection(xx); + +} + + + + +static void dumpSequence(EdStruct *xx, int seq, int pos, int width, char *str) +/* +** get part of a sequence from its `pos' base for `width' bases +** Bases number from 0? +*/ +{ + char *src; + int length = DBgetLength(xx,seq); + int i,j; + + src = DBgetSeq(xx,seq); + + /* Lefthand cut off */ + if (pos<0) { + i = (width<-pos)?width:-pos; + getLCut(xx,seq, -pos, i, str); + for(j=0;jrulerDisplayed) { + char *k; + int j,lower,times; + lower = (pos - pos%10); + times = width/10 + 2; + for (j=0,k=spare;jshowDifferences) { + int j; + for (j=0;jcursorSeq = 0; + xx->cursorPos = pos; + + for (i=1; i<=xx->DB_gelCount; i++) { + if (DBgetNumber(xx,i) == seq) { + xx->cursorSeq = i; + xx->cursorPos = pos; + break; + } + } + + /* + ** Display. + ** set xx->displayPos to force repositioning of cursor + */ + xx->displayPos = positionInContig(xx,xx->cursorSeq,xx->cursorPos) + + 2*xx->displayWidth; + redisplayWithCursor(xx); + return 0; +} + + +void incDisplayPos (EdStruct *xx, int distance) +/* +** Increase the leftmost base position on the screen by a symbolic ammount +*/ +{ + if (editorLocked()) { + incDisplayPosP(intToEdStruct(0), distance); + incDisplayPosP(intToEdStruct(1), distance); + } else + incDisplayPosP(xx, distance); + + redisplayDisagreement(); +} + + +void decDisplayPos (EdStruct *xx, int distance) +/* +** Decrease the leftmost base position on the screen by a symbolic ammount +*/ +{ + if (editorLocked()) { + decDisplayPosP(intToEdStruct(0), distance); + decDisplayPosP(intToEdStruct(1), distance); + } else + decDisplayPosP(xx, distance); + + redisplayDisagreement(); +} + + + + + +void setDisplayPosPercent (EdStruct *xx, float percent) +/* +** Set the leftmost base position on the screen given a percentage +*/ +{ + if (editorLocked()) { + setDisplayPosPercentP(intToEdStruct(0),percent); + setDisplayPosPercentP(intToEdStruct(1),percent); + } else + setDisplayPosPercentP(xx,percent); + + redisplayDisagreement(); +} + +void setDisplayPos(EdStruct *xx, int pos) +/* +** centralise pos on screen +*/ +{ + if (pos==1) pos = 2; /*if pos==1 no redraw is done. So force*/ + + if (editorLocked()) { + int offset = editorLockedPos(0/*don't force recalculation*/); + EdStruct *otherxx; + otherxx = intToEdStruct(0); + if (otherxx == xx) { + otherxx = intToEdStruct(1); + otherxx->displayPos = pos + offset; + } else { + otherxx->displayPos = pos - offset; + } + } + + xx->displayPos = pos; + decDisplayPos(xx,D_halfScreen); + +} + +static void showCursor(EdStruct *xx, int seq, int pos) +/* +** ensure that the cursor is visible on the screen +*/ +{ + if (onScreen(xx,seq,pos)) + positionCursor(xx,xx->sequencesWid,seq,pos); + else { + setDisplayPos(xx,positionInContig(xx,seq,pos)); +/* + if (xx->displayPos > positionInContig(xx,seq,pos)) + decDisplayPos(xx,D_halfScreen); + else + incDisplayPos(xx,D_halfScreen); + xx->displayPos = positionInContig(xx,seq,pos) - (xx->displayWidth/2); + if (xx->displayPos < 1) xx->displayPos = 1; + if (xx->displayPos > (DBgetLength(xx,0)+2 - xx->displayWidth)) + xx->displayPos = (DBgetLength(xx,0)+2 - xx->displayWidth); + redisplaySequences (xx,xx->namesWid, xx->sequencesWid, xx->displayPos, xx->displayWidth); +*/ + } +} + +void redisplayWithCursor(EdStruct *xx) +/* +** Redisplay screen, ensuring cursor display +*/ +{ + if (onScreen(xx,xx->cursorSeq,xx->cursorPos)) { + redisplaySequences (xx,xx->namesWid, xx->sequencesWid, xx->displayPos, xx->displayWidth); + redisplayDisagreement(); + } else + showCursor(xx,xx->cursorSeq,xx->cursorPos); +} + +void caretRight (Widget widget, XEvent *event, String *params, + Cardinal *num_params) +/* +** Move cursor right +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(widget)); + + if (xx->cursorPos<=DBgetLength(xx,xx->cursorSeq)) { + xx->cursorPos++; + showCursor(xx,xx->cursorSeq, xx->cursorPos); + } else + bell(); + +} + + +void caretLeft (Widget widget, XEvent *event, String *params, + Cardinal *num_params) +/* +** Move cursor left +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(widget)); + + + if (xx->cursorPos>1) { + xx->cursorPos--; + showCursor(xx,xx->cursorSeq, xx->cursorPos); + } else + bell(); + +} + +void caretDown (Widget widget, XEvent *event, String *params, + Cardinal *num_params) +/* +** Move cursor down, +** cycle if necessary +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(widget)); + + int *seqList,seqCount; + int posInContig; + int i; + + posInContig = positionInContig(xx,xx->cursorSeq,xx->cursorPos); + seqList = sequencesInRegion(xx,posInContig-1,2); + seqCount = linesInRegion(xx,posInContig-1,2); + for(i=0; + icursorSeq; + i++); + + i++; + if (i == seqCount) i = 0; + + xx->cursorSeq = seqList[i]; + xx->cursorPos = posInContig - DBgetRelPos(xx,xx->cursorSeq) + 1; + if (xx->cursorPos<1) + xx->cursorPos = 1; + else + if (xx->cursorPos > DBgetLength(xx,xx->cursorSeq)+1) + xx->cursorPos = DBgetLength(xx,xx->cursorSeq)+1; + showCursor(xx,xx->cursorSeq, xx->cursorPos); + +} + +void caretUp (Widget widget, XEvent *event, String *params, + Cardinal *num_params) +/* +** Move cursor up, +** cycle if necessary +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(widget)); + + int *seqList,seqCount; + int posInContig; + int i; + + posInContig = positionInContig(xx,xx->cursorSeq,xx->cursorPos); + seqList = sequencesInRegion(xx,posInContig-1,2); + seqCount = linesInRegion(xx,posInContig-1,2); + for(i=0; + icursorSeq; + i++); + + if (i==0) i = seqCount; + i--; + + xx->cursorSeq = seqList[i]; + xx->cursorPos = posInContig - DBgetRelPos(xx,xx->cursorSeq) + 1; + if (xx->cursorPos<1) + xx->cursorPos = 1; + else + if (xx->cursorPos > DBgetLength(xx,xx->cursorSeq)+1) + xx->cursorPos = DBgetLength(xx,xx->cursorSeq)+1; + showCursor(xx,xx->cursorSeq, xx->cursorPos); + +} + + +static void deleteBase (EdStruct *xx, int seq, int pos) +/* +** delete a single base in a sequence +** and update the screen if necessary +*/ +{ + if (seq && pos) { + int j; + char *s; + char deletedBase; + s=DBgetSeq(xx,seq); + deletedBase = s[pos-1]; + for (j = pos; j < DBgetLength(xx,seq); j++) + s[j-1] = s[j]; + DBsetLength(xx,seq,DBgetLength(xx,seq)-1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_SEQ_MODIFIED|DB_FLAG_REL_MODIFIED); + tagDeleteBase(xx,seq,pos,&deletedBase); + selectDeleteBase(xx, seq, pos); + } + else if (seq) { + /* + ** deletion at position 0 - shift left sequence + */ + int i,j; + + if (DBgetRelPos(xx,seq)==1) { + for (i=1; i <= xx->DB_gelCount; i++) { + if (seq == xx->DBorder[i]) { + for (j=i;j>1;j--) + xx->DBorder[j] = xx->DBorder[j-1]; + xx->DBorder[1] = seq; + } else + DBsetRelPos(xx,xx->DBorder[i],DBgetRelPos(xx,xx->DBorder[i])+1); + } + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_REL_MODIFIED); + + } else { + DBsetRelPos(xx,seq,DBgetRelPos(xx,seq)-1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_REL_MODIFIED); + + for (i=1; seq != xx->DBorder[i] && i <= xx->DB_gelCount; i++); + for (j=i; DBgetRelPos(xx,xx->DBorder[j-1]) > DBgetRelPos(xx,seq) ;j--) + xx->DBorder[j] = xx->DBorder[j-1]; + xx->DBorder[j] = seq; + } + + } + +} + +void deleteKey (Widget widget, XEvent *event, String *params, + Cardinal *num_params) +/* +** delete a character +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(widget)); + + int i; + char ch; + + if (!(xx->DB_flags & DB_ACCESS_UPDATE)) return; + if (! onScreen(xx, xx->cursorSeq, xx->cursorPos)) { + /* + ** When cursor is currently displayed on the screen, + ** give a warning tone, and recentre screen on cursor + */ + showCursor(xx,xx->cursorSeq, xx->cursorPos); + bell(); + } else if (positionInContig(xx,xx->cursorSeq,xx->cursorPos)==1 && !xx->cursorSeq) { + bell(); + } else if (xx->cursorSeq) { + if (editModeIsSuperman()) { + if (xx->cursorPos==1) + ch = ' '; + else { + char *s; + s = DBgetSeq(xx,xx->cursorSeq); + ch = s[xx->cursorPos-2]; + } + + recordEdit(&bucketStack,xx,undoDelete,xx->cursorSeq,xx->cursorPos-1,ch,0); + + deleteBase (xx,xx->cursorSeq, xx->cursorPos-1); + + calculateConsensusLength(xx); + + if (xx->cursorPos>1) xx->cursorPos--; + redisplayWithCursor(xx); + + } else bell(); + } else { + char consensus[2]; + DBcalcConsensus(xx,xx->cursorPos-1,1,consensus,BOTH_STRANDS); + if (*consensus != '*') { + /* + ** Only allow deletion from consensus when it's an asterisk + */ + bell(); + } else { + recordEdit(&bucketStack,xx,undoMark,0,xx->cursorPos,'\0',0); + for (i=1; i<=xx->DB_gelCount; i++) { + if (DBgetRelPos(xx,i) + DBgetLength(xx,i) <= xx->cursorPos) ; + else if (DBgetRelPos(xx,i) >= xx->cursorPos) { + recordEdit(&bucketStack,xx,undoDelete,i,0,' ',0); + deleteBase (xx,i, 0); + } + else { + char *s; + s=DBgetSeq(xx,i); + ch = s[xx->cursorPos-DBgetRelPos(xx,i)-1]; + recordEdit(&bucketStack,xx,undoDelete,i,xx->cursorPos-DBgetRelPos(xx,i),ch,0); + deleteBase (xx,i, xx->cursorPos-DBgetRelPos(xx,i)); + } + + } + recordEdit(&bucketStack,xx,undoMark,0,0,'\0',0); + + DBsetLength(xx,0,DBgetLength(xx,0)-1); + redisplaySequences (xx,xx->namesWid, xx->sequencesWid, xx->displayPos, xx->displayWidth); + redisplayDisagreement(); + xx->cursorPos--; + redisplayWithCursor(xx); + } + } + +} + +static void insertBase (EdStruct *xx, int seq, int pos, char base) +/* +** insert a single base in a sequence +** and update the screen if necessary +*/ +{ + if (seq && base!=' ') { + int j; + char *s; + int len = DBgetLength(xx,seq); + /* don't let user overrun maximum gel length */ + if (len<(int)*saveState.maxgel) { + s=DBgetSeq(xx,seq); + for (j = len+1; j > pos; j--) + s[j-1] = s[j-2]; + s[pos-1] = base; + DBsetLength(xx,seq,len+1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_SEQ_MODIFIED|DB_FLAG_REL_MODIFIED); + tagInsertBase(xx,seq,pos,&base); + selectInsertBase(xx, seq, pos); + } + } + else if (seq) { + /* + ** space inserted at start: shift sequence right + */ + int i,j; + + if (DBgetRelPos(xx,seq)==1) + for (i=1; iDB_gelCount && DBgetRelPos(xx,xx->DBorder[i+1])==1;i++); + else + i=0; + if (i==1) { + for (i=2; i <= xx->DB_gelCount; i++) { + DBsetRelPos(xx,xx->DBorder[i],DBgetRelPos(xx,xx->DBorder[i])-1); + } + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_REL_MODIFIED); + + } else { + DBsetRelPos(xx,seq,DBgetRelPos(xx,seq)+1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_REL_MODIFIED); + + for (i=1; seq != xx->DBorder[i] && i <= xx->DB_gelCount; i++); + for (j=i; j < xx->DB_gelCount && DBgetRelPos(xx,xx->DBorder[j+1]) < DBgetRelPos(xx,seq) ; j++) + xx->DBorder[j] = xx->DBorder[j+1]; + xx->DBorder[j] = seq; + } + + } + +} + +static void insertChar (EdStruct *xx,int seq, int pos, char key) +/* +** insert a character +*/ +{ + int i; + + /* Insert the base to the right of the caret */ + /* perform a check to see that sequences aren't too long */ + if (seq) { + /* don't let user overrun maximum gel length */ + if (editModeIsSuperman() && + DBgetLength(xx,seq)<(int)*saveState.maxgel) { + + recordEdit(&bucketStack,xx,undoInsert,seq,pos,key,0); + + insertBase(xx, seq, pos, key); + + calculateConsensusLength(xx); + + if (key!=' ') xx->cursorPos = ++pos; + redisplayWithCursor(xx); + } else bell(); + } else if (key == '*') { + /* + ** Only allow insertion of asterisks from consensus + */ + recordEdit(&bucketStack,xx,undoMark,0,pos,'\0',0); + for (i=1; i<=xx->DB_gelCount; i++) { + int len = DBgetLength(xx,i); + int relPos = DBgetRelPos(xx,i); + if (relPos + len < pos || + len>=(int)*saveState.maxgel) ; + else if (relPos > pos) { + recordEdit(&bucketStack,xx,undoInsert,i,0,key,0); + insertBase(xx, i,0,' '); + } + else if (len) { + recordEdit(&bucketStack,xx,undoInsert,i, pos-relPos+1,key,0); + insertBase(xx, i, pos-relPos+1, key); + } + } + recordEdit(&bucketStack,xx,undoMark,0,0,'\0',0); + + DBsetLength(xx,0,DBgetLength(xx,0)+1); + + xx->cursorPos = ++pos; + redisplayWithCursor(xx); + } else { + bell(); + } +} + +static void replaceBase (EdStruct *xx, int seq, int pos, char base) +/* +** replace a single base in a sequence +** and update the screen if necessary +*/ +{ + if (seq) { + char *s; + char deletedBase; + s=DBgetSeq(xx,seq); + deletedBase=s[pos-1]; + s[pos-1] = base; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_SEQ_MODIFIED); + tagReplaceBase(xx,seq,pos,&deletedBase, &base); + } + +} + +static void replaceChar (EdStruct *xx, int seq, int pos, char key) +/* +** replace a character +*/ +{ + /* int i,j; */ + char ch; + + /* + ** Don't allow replacing past end of sequence + */ + if (DBgetLength(xx,seq) < pos) { + bell(); + return; + } + if (seq) { + char *s; + s=DBgetSeq(xx,seq); + + ch = s[pos-1]; + recordEdit(&bucketStack,xx,undoReplace,seq,pos,ch,0); + + replaceBase(xx,seq, pos, key); + + xx->cursorPos = ++pos; + redisplayWithCursor(xx); + + } else { + bell(); + /* + ** Don't allow replacements on consensus at ANY stage + ** + ** recordEdit(&bucketStack,xx,undoMark,0,pos,'\0',0); + ** for (i=1; i<=xx->DB_gelCount; i++) { + ** if (DBgetRelPos(xx,i) + DBgetLength(xx,i) <= pos) ; + ** else if (DBgetRelPos(xx,i) > pos) ; + ** else if (DBgetLength(xx,i)) { + ** char *s; + ** s=DBgetSeq(xx,i); + ** ch = s[pos-DBgetRelPos(xx,i)]; + ** recordEdit(&bucketStack,xx,undoReplace,i,pos-DBgetRelPos(xx,i)+1,ch,0); + ** replaceBase(xx,i, pos-DBgetRelPos(xx,i)+1, key); + ** } + ** } + ** recordEdit(&bucketStack,xx,undoMark,0,0,'\0',0); + ** + ** + ** xx->cursorPos = ++pos; + ** redisplayWithCursor(xx); + */ + } +} + +static int validKey(char key) +{ + static char validKeys[] = "CcTtAaGg1234DVBHKLMNRY5678-*"; + if (*saveState.idm == 26) + return (key!=' '); + else + return (strchr(validKeys,key) != NULL); +} + +void keyPress (Widget widget, XEvent *event, String *params, + Cardinal *num_params) +/* +** Handle a key press +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(widget)); + + char keys[8]; + char key; + KeySym keySym; + int charCount; + XComposeStatus compose; + /* + ** validkeys is different when database is for DNA and when + ** database is for protein + ** + ** switch (xx->DB_flags & DB_DATA_TYPE) { + ** case DB_DATA_TYPE_DNA: do dna stuff + ** case DB_DATA_TYPE_PROTEIN: do protein stuff + ** default : do dna stuff??? + ** } + ** BASES = 6 for dna, 26 for protein (specified by fortran variable IDM?) + static char validKeys[] = "CcTtAaGg1234DVBHKLMNRY5678-*"; + */ + + if (!(xx->DB_flags & DB_ACCESS_UPDATE)) return; + + charCount = XLookupString((XKeyEvent *)event, keys, + (int)sizeof(keys), &keySym,&compose); + key = keys[0]; + if (charCount==1) { + if (! onScreen(xx, xx->cursorSeq, xx->cursorPos)) { + /* + ** When cursor is currently displayed on the screen, + ** give a warning tone, and recentre screen on cursor + */ + showCursor(xx,xx->cursorSeq, xx->cursorPos); + bell(); + } else if (validKey(key)) { + if (editModeIsInsert()) + insertChar (xx,xx->cursorSeq, xx->cursorPos, key); + else + replaceChar (xx,xx->cursorSeq, xx->cursorPos, key); + } + else { + if (xx->cursorSeq && xx->cursorPos==1 && key == ' ' && editModeIsInsert()) + insertChar (xx,xx->cursorSeq, xx->cursorPos, key); + else bell(); + } + } +} + +void buttonDown (Widget widget, XEvent *event, String *params, + Cardinal *num_params) +/* +** An attempt has been made to reposition the cursor +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(widget)); +#define left_margin 4 +#define top_margin 4 + + int x,y; + int *seqList; + + if (editorState == StateDown) return; + + if (widget != xx->sequencesWid) return; + + x = (event->xbutton.x-left_margin) / xx->fontWidth; + y = (event->xbutton.y-top_margin) / xx->fontHeight - xx->rulerDisplayed; + + + if (y>=0 && y < xx->displayHeight && x>=0 && x < xx->displayWidth) { + seqList = sequencesOnScreen(xx,xx->displayPos,xx->displayWidth); + xx->cursorSeq = seqList[y]; + xx->cursorPos = xx->displayPos - DBgetRelPos(xx,xx->cursorSeq) + x + 1; + if (xx->cursorPos<1) + xx->cursorPos = 1; + else + if (xx->cursorPos > DBgetLength(xx,xx->cursorSeq)+1) + xx->cursorPos = DBgetLength(xx,xx->cursorSeq)+1; + positionCursor(xx,widget,xx->cursorSeq,xx->cursorPos); + } + +} + +static void undoLastEdit (EdStruct *xx, int command, int seq, int pos, char ch, int t) +/* +** Undo a command +*/ +{ + switch (command) { + case undoDelete: + insertBase(xx, seq,pos,ch); + break; + case undoInsert: + deleteBase(xx,seq,(ch==' ')?0:pos); + break; + case undoReplace: + replaceBase(xx,seq,pos,ch); + break; + case undoExtend: + unextend(xx,seq,ch); + break; + case undoUnextend: + undo_unextend(xx,seq,ch,t); + break; + default: + break; + } +} + +void undoLastCommand() +/* +** Undo last keypress that modified contig +*/ +{ + EdStruct *xx; + int command; + int seq; + int pos; + char ch; + int t; + + retrieveEdit(&bucketStack,&xx,&command,&seq,&pos,&ch,&t); + if (command == undoNullCommand) { + bell(); + return; + } + else if (command == undoMark) { + + retrieveEdit(&bucketStack,&xx,&command,&seq,&pos,&ch,&t); + while (command != undoMark) { + undoLastEdit(xx,command,seq,pos,ch,t); + retrieveEdit(&bucketStack,&xx,&command,&seq,&pos,&ch,&t); + } + + xx->cursorSeq = 0; + xx->cursorPos = pos; + + } else { + + undoLastEdit(xx,command,seq,pos,ch,t); + + xx->cursorSeq = seq; + xx->cursorPos = pos+(command==undoDelete); + + } + + calculateConsensusLength(xx); + redisplayWithCursor(xx); +} + +static int showTrace(EdStruct *xx, int seq, int pos, int baseSpacing) +{ + + char fileName[200]; + tagStruct *t; + /* + int traceNo; + */ + int t_len; /* number of bases in trace */ + int t_lcut; /* left cut off of usable data */ + int t_ulen; /* length of usable data */ + char t_type[5]; /* type of trace */ + char t_fname[FILE_NAME_LENGTH+1]; /* file name of trace */ + char *rawData; + int baseNum; + + t = (tagStruct *) DBgetTags(xx,seq); + + force_comment(t); + + if (! t->newcommentlen) return 1; + sscanf(t->newcomment,"%6d%6d%6d%*s",&t_len,&t_lcut,&t_ulen); + strncpy(t_type,&t->newcomment[18],4); + strncpy(t_fname,&t->newcomment[22],FILE_NAME_LENGTH); + + t_type[4] = '\0'; + { /* convert fortran string to c string */ + int i; + for (i=FILE_NAME_LENGTH-1;i>=0 && (!t_fname[i] || isspace(t_fname[i]));i--) ; + t_fname[++i] = '\0'; + } + + /* skip if no raw data file for trace */ + if (t_fname[0] == '\0') return 1; + + /* check trace file exists */ + if ( ! file_exists(t_fname) ) { + char *s; + /* try in rawData */ + /* + ** get environment details + */ + rawData = (char *)getenv ("RAWDATA"); + if (rawData == NULL) return 1; + /* + sprintf (fileName,"%s/%s",rawData,t_fname); + */ + if ((s=findfile(t_fname,rawData))==NULL) return 1; + strcpy (fileName,s); + } else + /* ok */ + strcpy (fileName,t_fname); + + if (DBgetComp(xx,seq) == UNCOMPLEMENTED) { + baseNum = origpos(xx, seq, pos) + t_lcut; + manageTrace(t_type, fileName, baseNum, t_lcut, t_ulen, /*not complemented*/0, baseSpacing, DBgetName(xx,seq)); + } else { + baseNum = t_len - t_lcut - origpos(xx, seq, normalisePos(xx,seq,pos,1)); + manageTrace(t_type, fileName, baseNum, t_lcut, t_ulen, /*complemented*/1, baseSpacing, DBgetName(xx,seq)); + } + + + return 0; +} + +void invokeTrace (Widget widget, XEvent *event, String *params, + Cardinal *num_params) +{ + EdStruct *xx = widgetToEdStruct(XtParent(widget)); + int baseSpacing = xx->fontWidth * 2; + + if (xx->cursorSeq) { + showTrace( + xx, + xx->cursorSeq, + xx->cursorPos, + baseSpacing); + } else { + int *seqList; + int i; + seqList = sequencesOnScreen(xx,xx->cursorPos,1); + for (i=0; idisplayHeight && seqList[i]; i++) + showTrace( + xx, + seqList[i], + xx->cursorPos-DBgetRelPos(xx,seqList[i])+1, + baseSpacing); + + } +} + +void countDisagreements(int *overlapLength, int *wingeCount) +{ + int left0,right0; + int left1/*,right1*/; + int length0,length1; + int offset = editorLockedPos(1/*force recalculation*/); + int i; + EdStruct *xx[2]; + char *ol0,*ol1; + + xx[0] = intToEdStruct(0); + xx[1] = intToEdStruct(1); + + if (offset < 0) { + left0 = 1-offset; + left1 = 1; + } else { + left0 = 1; + left1 = 1+offset; + } + length0 = DBgetLength(xx[0],0); + length1 = DBgetLength(xx[1],0); + if (offset+length0 < length1) { + right0 = length0; + /* + right1 = length0-offset; + */ + } else { + right0 = length1-offset; + /* + right1 = length1; + */ + } + *overlapLength = right0 - left0+1; + *wingeCount = 0; + + if (*overlapLength > 0) { + ol0 = (char *) malloc(*overlapLength+1); + ol1 = (char *) malloc(*overlapLength+1); + DBcalcConsensus(xx[0],left0,*overlapLength,ol0,BOTH_STRANDS); + DBcalcConsensus(xx[1],left1,*overlapLength,ol1,BOTH_STRANDS); + for (i=0;i<*overlapLength;i++) if(ol0[i]!=ol1[i])(*wingeCount)++; + free(ol0); + free(ol1); + } +} + + +void selectRead (Widget widget, XEvent *event, String *params, + Cardinal *num_params) +/* +** A gel reading name has been selected - toggle highlighting +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(widget)); +#define left_margin 4 +#define top_margin 4 + + int y; + int *seqList; + + if (editorState == StateDown) return; + + if (widget != xx->namesWid) return; + + y = (event->xbutton.y-top_margin) / xx->fontHeight - xx->rulerDisplayed; + + if (y>=0 && y < xx->displayHeight) { + int flag; + seqList = sequencesOnScreen(xx,xx->displayPos,xx->displayWidth); + flag = DBgetFlags(xx,seqList[y]); + flag ^= DB_FLAG_SELECTED; + DBsetFlags(xx,seqList[y],flag); + redisplaySequences (xx,xx->namesWid, xx->sequencesWid, xx->displayPos, xx->displayWidth); + } + +} + + + + +/************************************************************* +** +** Fast accessing routines... utilising binary search +** +*************************************************************/ + +int posToIndex(EdStruct *xx, int pos) +/* +** Find the first sequence that starts at or to the right of a +** given position +*/ +{ + int Min, Max, Mid; + + /* binary search */ + /* Min, Max, Mid refer to pairs of numbers: ie MAX --> [MAX-1],[MAX] */ + Min = 1; + Max = xx->DB_gelCount + 1; + + do { + int r1,r2; + + Mid = (Max+Min)/2; + + /* compare */ + r1 = (Mid==1)?(pos-1):DBgetRelPos(xx,xx->DBorder[Mid-1]); + r2 = (Mid==xx->DB_gelCount+1)?(pos+1):DBgetRelPos(xx,xx->DBorder[Mid]); + + if (r1 < pos && r2 >= pos) + return (Mid==xx->DB_gelCount+1)?(0):Mid; + + if (r1 < pos) + Min = Mid+1; + else + Max = Mid-1; + + } while (Max>=Min); + + return 0; + +} + + +int posToSeq(EdStruct *xx, int pos) +/* +** Find the first sequence that starts at or to the right of a +** given position +*/ +{ + int ind; + + ind = posToIndex(xx,pos); + if (ind) + return xx->DBorder[ind]; + else + return 0; + +} + + +int seqToIndex(EdStruct *xx, int seq) +/* +** +*/ +{ + int i; + int ind; + + ind = posToIndex(xx,DBgetRelPos(xx,seq)); + + if (ind) { + for (i=ind; i<=xx->DB_gelCount && xx->DBorder[i]!=seq;i++) ; + + if (i<=xx->DB_gelCount) return i; + } + + return 0; + +} + + + + diff --git a/src/staden/edUtils.h b/src/staden/edUtils.h new file mode 100644 index 0000000..cc3b1a4 --- /dev/null +++ b/src/staden/edUtils.h @@ -0,0 +1,257 @@ +#include +#include "tagUtils.h" +#include "fort.h" + +#ifndef _edUtils_h +#define _edUtils_h + +/* +** constant definitions +*/ +#define MAX_DISPLAY_WIDTH 200 +#define DEFAULT_DISPLAY_WIDTH 80 +#define DB_NAMELEN 12 /* size of records in AR file */ +#define DB_GELNOLEN 5 +#define NAMELEN (DB_NAMELEN + DB_GELNOLEN + 1) +#define BASES 6 + +/* +** Useful distances +** (treat as symbolic rather than actual distances) +*/ +#define D_screen 80 +#define D_halfScreen 40 +#define D_character 1 + +/* +** Flags for the internal database +*/ +/* for whole database */ +#define DB_ACCESS (1L<<0) +#define DB_DATA_TYPE (1L<<1) +#define DB_STORAGE (1L<<2) +#define DB_ACCESS_READONLY (0L) +#define DB_ACCESS_UPDATE (DB_ACCESS) +#define DB_DATA_TYPE_DNA (0L) +#define DB_DATA_TYPE_PROTEIN (DB_DATA_TYPE) +#define DB_STORAGE_DISK (0L) +#define DB_STORAGE_INTERNAL (DB_STORAGE) +#define DB_DELAYED_READ (0L) +/* for each sequence */ +#define DB_FLAG_NONE (0L) +#define DB_FLAG_IN_MEMORY (1L<<0) +#define DB_FLAG_SEQ_MODIFIED (1L<<1) +#define DB_FLAG_REL_MODIFIED (1L<<2) +#define DB_FLAG_TAG_MODIFIED (1L<<3) +#define DB_FLAG_SELECTED (1L<<4) +#define DB_FLAG_TAG_IN_MEMORY (1L<<5) +#define DB_FLAG_SEQ_IN_MEMORY (1L<<0) +#define DB_FLAG_NAME_IN_MEMORY (1L<<6) +/* +** type definitions +*/ +typedef struct { + int relPos; + int length; + int number; + int complemented; + char *name; + char *sequence; + long flags; + tagStruct *tagList; + } DBStruct, *DBptr; + + +/* REMEMBER TO: update structure initialisation in edUtils.c */ +typedef struct { + int oldMax; + long DB_flags; + int DB_gelCount; + int DB_contigNum; + int *DBlist; + int *DBorder; + DBStruct *DB; + int displayPos ; + int displayWidth; + int displayHeight; + int cursorPos; + int cursorSeq; + int rulerDisplayed; + int consensusDisplayed; + int fontWidth; + int fontHeight; + Widget edWid; + Widget namesWid; + Widget sequencesWid; + Widget scrollButtonsWid; + Widget sliderWid; + char displayedConsensus[ MAX_DISPLAY_WIDTH ]; + int select_made; + int select_seq; + int select_start_pos; + int select_end_pos; + tagStruct *select_tag; + int reveal_cutoffs; + int showDifferences; + } EdStruct, *EdStructPtr; +/* REMEMBER TO: update structure initialisation in edUtils.c */ + + +/* +** Useful macros concerning internal database +*/ +#define DBgetRelPos(X,A) ((X)->DB[(A)].relPos) +#define DBgetLength(X,A) ((X)->DB[(A)].length) +#define DBgetNumber(X,A) ((X)->DB[(A)].number) +#define DBgetComp(X,A) ((X)->DB[(A)].complemented) +#define DBgetFlags(X,A) ((X)->DB[(A)].flags) +extern char *DBgetSeq(); +extern tagStruct *DBgetTags(); +extern char *DBgetName(); + +/****************/ +#define DB_RelPos(X,A) ((X)->DB[(A)].relPos) +#define DB_Length(X,A) ((X)->DB[(A)].length) +#define DB_Number(X,A) ((X)->DB[(A)].number) +#define DB_Comp(X,A) ((X)->DB[(A)].complemented) +#define DB_Name(X,A) ((X)->DB[(A)].name) +#define DB_Seq(X,A) ((X)->DB[(A)].sequence) +#define DB_Flags(X,A) ((X)->DB[(A)].flags) +#define DB_Tags(X,A) ((X)->DB[(A)].tagList) +/****************/ +#define COMPLEMENTED -1 +#define BOTH_STRANDS 0 +#define UNCOMPLEMENTED 1 +#define DBgetGelName(xx,i) ( &( DBgetName(xx,i) )[DB_GELNOLEN+1] ) + +#define DBsetRelPos(X,A,B) (X)->DB[(A)].relPos = (B) +#define DBsetLength(X,A,B) (X)->DB[(A)].length = (B) +#define DBsetNumber(X,A,B) (X)->DB[(A)].number = (B) +#define DBsetComp(X,A,B) (X)->DB[(A)].complemented = (B) +#define DBsetName(X,A,B) (X)->DB[(A)].name = (B) +#define DBsetSeq(X,A,B) (X)->DB[(A)].sequence = (B) +#define DBsetFlags(X,A,B) (X)->DB[(A)].flags = (B) +#define DBsetTags(X,A,B) (X)->DB[(A)].tagList = (B) + +/* +** Useful macros +*/ +#define normalisePos(X,S,P,L) \ + ( (DBgetComp((X),(S))==UNCOMPLEMENTED) ? (P) : (DBgetLength((X),(S)) - (P) - (L) + 2) ) + + +/* +** External definitions +*/ +extern int initialiseDB( + EdStruct *xx, + int_f *idevr, /* unit number for relationships */ + int_f *idevw, /* unit number for working versions of sequences */ + int_f *idevn, /* unit number for sequence names */ + int_f *relpg, /* relative positions of gels in sequences */ + int_f *lngthg, /* lengths of sequences */ + int_f *lnbr, /* left neighbours */ + int_f *rnbr, /* right neighbours */ + int_f *maxgel, /* maximum length of gel */ + int_f *idbsiz, /* size of database */ + int_f *llino /* left-most gel in contig */ + ); + +extern void saveDB( + EdStruct *xx, + int_f *idevr, /* unit number for relationships */ + int_f *idevw, /* unit number for working versions of sequences */ + int_f *idevn, /* unit number for sequence names */ + int_f *relpg, /* relative positions of gels in sequences */ + int_f *lngthg, /* lengths of sequences */ + int_f *lnbr, /* left neighbours */ + int_f *rnbr, /* right neighbours */ + int_f *maxgel /* maximum length of gel */ + ); + +extern void joinDB( + int_f *idevr, /* unit number for relationships */ + int_f *idevw, /* unit number for working versions of sequences */ + int_f *idevn, /* unit number for sequence names */ + int_f *relpg, /* relative positions of gels in sequences */ + int_f *lngthg, /* lengths of sequences */ + int_f *lnbr, /* left neighbours */ + int_f *rnbr, /* right neighbours */ + int_f *maxgel, /* maximum length of gel */ + int_f *llinol, + int_f *lnconl, + int_f *llinor, + int_f *lnconr, + int_f *ngels, + int_f *nconts, + int_f *idbsiz + ); + + +extern void freeDB(EdStruct *xx); +extern void countDisagreements(int *overlapLength, int *wingeCount); + +extern int createEdDisplay(EdStruct *xx,Widget namesWid, Widget sequencesWid,int seq,int pos); + +extern void incDisplayPos(EdStruct *xx, int distance); +extern void decDisplayPos(EdStruct *xx, int distance); +extern void setDisplayPos(EdStruct *xx, int pos); +extern void setDisplayPosPercent(EdStruct *xx, float percent); + +extern int posToIndex(EdStruct *xx, int pos); + +extern void undoLastCommand(); +extern void saveDatabase(EdStruct *xx); + +extern void caretRight(Widget, XEvent *, String *, Cardinal *); +extern void caretLeft(Widget, XEvent *, String *, Cardinal *); +extern void caretDown(Widget, XEvent *, String *, Cardinal *); +extern void caretUp(Widget, XEvent *, String *, Cardinal *); +extern void deleteKey(Widget, XEvent *, String *, Cardinal *); +extern void keyPress(Widget, XEvent *, String *, Cardinal *); +extern void buttonDown(Widget, XEvent *, String *, Cardinal *); +extern void invokeTrace(Widget, XEvent *, String *, Cardinal *); +extern void selectRead(Widget, XEvent *, String *, Cardinal *); + +extern EdStructPtr intToEdStruct(int i); +extern EdStructPtr widgetToEdStruct(Widget w); +extern EdStructPtr getFreeEdStruct(); + +extern void DBgetSequence(EdStruct *xx, int seq, int pos, int width, char *str); +/* +** get part of a sequence from its `pos' base for `width' bases +** Bases number from 0? +*/ +extern int *sequencesInRegion(EdStruct *xx,int pos, int width); +/* +** Return a pointer to list of sequences in region of contig +*/ +extern int *sequencesOnScreen(EdStruct *xx,int pos, int width); +/* +** Return a pointer to list of sequences on screen +*/ +extern int positionInContig(EdStruct *xx, int seq, int pos); +/* +** returns relative position in a sequence as an +** absolute position in the contig +*/ +extern void DBcalcConsensus (EdStruct *xx,int pos, int width, char *str, int strand); +/* +** calculate the consensus for position `pos' in contig, +** for `width' characters. Take into accound readings on `strand' only: +** +*/ +extern void redisplaySequences (EdStruct *xx, Widget namesWid, Widget sequencesWid, int pos, int width); +/* +** Redisplay the whole sequence display +*/ +extern void redisplayWithCursor(EdStruct *xx); +/* +** Redisplay screen, ensuring cursor display +*/ +void calculateConsensusLength(EdStruct *xx); +/* +** Calculate dynamic consensus length +*/ + +#endif /* _edUtils_h */ diff --git a/src/staden/extend.c b/src/staden/extend.c new file mode 100644 index 0000000..5890e81 --- /dev/null +++ b/src/staden/extend.c @@ -0,0 +1,722 @@ +/* +** Change log: +** +** 1/10/91 SD Added calculateConsensusLength to extend, unextend, undo_unextend +** 29/4/92 SD Changes related to general speed up in edUtils.c +** 18/5/92 SD Construct (*nc=*++nc) not liked by dec +*/ + +#include "edUtils.h" +#include "fortran.h" +#include "tagUtils.h" +#include "contigEditor.h" +#include "undo.h" +#include "select.h" +#include +#include + +#define LEFT 1 +#define RIGHT 2 + + +tagStruct *force_get_tag(EdStruct *xx, int seq, char *type) +/* +** find a control tag of a particular type +** Create one if one doesn't exist +*/ +{ + tagStruct *t; + + t = (tagStruct *)findTagByType(xx,seq,type); + + if (t == NULL) { + /* + ** create one + */ + t = newTag(); + strncpy(t->tagrec.type.c,type,4); + t->flags = TAG_INSERTED; + insertTag(xx,seq,t); + } + + /* + ** Force comment to be in memory + */ + force_comment(t); + + return t; + +} + + + +int extend(EdStruct *xx, int seq, int dir, int time) +{ + int c = (DBgetComp(xx,seq)==COMPLEMENTED); + char t_type[4]; + tagStruct *t; + int use_tag; + char extend_char; + int l; + + if (DBgetLength(xx,seq) >= (int)*saveState.maxgel) return 1; + + use_tag = (c && dir==LEFT || !c && dir==RIGHT )?RIGHT: LEFT; + strncpy(t_type, + (use_tag==RIGHT )?TAG_TYPE_RCUT:TAG_TYPE_LCUT,4); + + /* + ** Find appropriate tag, + ** create one if one doesn't exist + */ + t = force_get_tag(xx,seq,t_type); + + /* + ** No cutoff - ignore request + */ + l = t->newcommentlen; + if (! l) + return 1; + + /* + ** Determine character to extend + */ + if (use_tag==LEFT) { + /* Get character off end of cutoff */ + extend_char = t->newcomment[l-1]; + t->newcomment[l-1]='\0'; + t->newcommentlen--; + } else { + /* Get character off start of cutoff */ + int i; + extend_char = t->newcomment[0]; + for (i=0;t->newcomment[i];i++) + t->newcomment[i] = t->newcomment[i+1]; + t->newcommentlen--; + } + t->flags |= TAG_COMMENT_CHANGED; + + /* + ** complement if necessary + */ + if (use_tag != dir) { + int_f i=1; + sqcom_(&extend_char,&i,(int_f)1); + } + + + if (dir==LEFT){ + int i,j; + char *s; + + /* + ** insert base at start of sequence + */ + s=DBgetSeq(xx,seq); + for (j = DBgetLength(xx,seq)+1; j > 1; j--) + s[j-1] = s[j-2]; + s[0] = extend_char; + DBsetLength(xx,seq,DBgetLength(xx,seq)+1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_SEQ_MODIFIED|DB_FLAG_REL_MODIFIED); + selectInsertBase(xx,seq,1); + + /* + ** shift sequence left + */ + if (DBgetRelPos(xx,seq)==1) { + for (i=1; i <= xx->DB_gelCount; i++) { + if (seq == xx->DBorder[i]) { + for (j=i;j>1;j--) + xx->DBorder[j] = xx->DBorder[j-1]; + xx->DBorder[1] = seq; + } else + DBsetRelPos(xx,xx->DBorder[i],DBgetRelPos(xx,xx->DBorder[i])+1); + } + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_REL_MODIFIED); + + } else { + DBsetRelPos(xx,seq,DBgetRelPos(xx,seq)-1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_REL_MODIFIED); + + for (i=1; seq != xx->DBorder[i] && i <= xx->DB_gelCount; i++); + for (j=i; DBgetRelPos(xx,xx->DBorder[j-1]) > DBgetRelPos(xx,seq) ;j--) + xx->DBorder[j] = xx->DBorder[j-1]; + xx->DBorder[j] = seq; + } + + } else { + /* + ** insert base at end of sequence + */ + char *s; + int l=DBgetLength(xx,seq); + s=DBgetSeq(xx,seq); + s[l] = extend_char; + DBsetLength(xx,seq,l+1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_SEQ_MODIFIED|DB_FLAG_REL_MODIFIED); + selectInsertBase(xx,seq,l+1); + } + + if (use_tag == LEFT) { + /* + ** shift position of all tags right 1 + */ + tagStruct *t; + t = (tagStruct *) DBgetTags(xx,seq); + while (t != NULL) { + if (!(t->flags & TAG_DELETED) && + t->tagrec.position) { + t->tagrec.position++; + t->flags |= TAG_POSITION_CHANGED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } + t = t->next; + } + } + + /* + ** Modify raw data stuff + */ + { + tagStruct *t; + char type[5]; + char name[FILE_NAME_LENGTH+1]; + int llen,lcut,wlen; + t = (tagStruct *) DBgetTags(xx,seq); + force_comment(t); + sscanf(t->newcomment,"%6d%6d%6d%*s",&llen,&lcut,&wlen); + strncpy(type,&t->newcomment[18],4); + strncpy(name,&t->newcomment[22],FILE_NAME_LENGTH); + wlen++; + if (use_tag==LEFT) lcut--; + sprintf(t->newcomment,"%6d%6d%6d",llen,lcut,wlen); + strncat(t->newcomment,type,4); + strncat(t->newcomment,name,FILE_NAME_LENGTH); + t->newcommentlen = (int)strlen(t->newcomment); + t->flags |= TAG_COMMENT_CHANGED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + + } + + if (dir == RIGHT) xx->cursorPos++; + + /* + ** Lengths are a changing + */ + calculateConsensusLength(xx); + + return 0; + +} + + + + + +int unextend(EdStruct *xx, int seq, int dir, int time) +{ + + int c = (DBgetComp(xx,seq)==COMPLEMENTED); + char t_type[4]; + tagStruct *t; + int use_tag; + char extend_char[2]; + char external_buff[100]; + char internal_buff[100]; + char *ec = extend_char; + char *eb = external_buff; + char *ib = internal_buff; + int l; + int end_char_inserted = 0; + + use_tag = (c && dir==LEFT || !c && dir==RIGHT )?RIGHT: LEFT; + strncpy(t_type, + (use_tag==RIGHT )?TAG_TYPE_RCUT:TAG_TYPE_LCUT,4); + + /* + ** Find appropriate tag, + ** create one if one doesn't exist + */ + t = force_get_tag(xx,seq,t_type); + + /* + ** Process tag information + ** collating *DE? flags and *INS flags + ** and adjusting other flags accordingly + */ + { + tagStruct *t; + int ipos; + + t = (tagStruct *) DBgetTags(xx,seq); + ipos = normalisePos(xx,seq,(dir==LEFT)?1:DBgetLength(xx,seq),1); + + while (t != NULL) { + if (!(t->flags & TAG_DELETED) && + t->tagrec.position == ipos && + strncmp(t->tagrec.type.c,TAG_TYPE_INSERT,3)==0) { + /* + ** Handle unextending past inserts + */ + end_char_inserted++; + t->flags |= TAG_DELETED; + t->time = time; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + }else if (!(t->flags & TAG_DELETED) && + strncmp(t->tagrec.type.c, TAG_TYPE_DELETE,3)==0) { + /* + ** Handle unextending past deletes + */ + if (use_tag==LEFT && t->tagrec.position == ipos || + use_tag==RIGHT && t->tagrec.position == (ipos+1)) { + *eb++ = t->tagrec.type.c[3]; + t->time = time; + t->flags |= TAG_DELETED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } else if (use_tag==RIGHT && t->tagrec.position == ipos) { + *ib++ = t->tagrec.type.c[3]; + t->time = time; + t->flags |= TAG_DELETED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } else if (use_tag == LEFT) { + t->tagrec.position--; + t->flags |= TAG_POSITION_CHANGED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } + } else if (!(t->flags & TAG_DELETED) && + t->tagrec.position <= ipos && + t->tagrec.position + t->tagrec.length > ipos){ + /* + ** Handle unextending past other tags + */ + t->tagrec.length--; + t->flags |= TAG_LENGTH_CHANGED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } else if (!(t->flags & TAG_DELETED) && + use_tag==LEFT && t->tagrec.position) { + /* + ** Handle all other positional adjustments + */ + t->tagrec.position--; + t->flags |= TAG_POSITION_CHANGED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } + t = t->next; + } + } + + if (dir==LEFT){ + int i,j; + char *s; + int l=DBgetLength(xx,seq); + + /* + ** get base at start of sequence + */ + s=DBgetSeq(xx,seq); + *ec++ = s[0]; + for (j = 2; j <= l; j++) + s[j-2] = s[j-1]; + DBsetLength(xx,seq,l-1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_SEQ_MODIFIED|DB_FLAG_REL_MODIFIED); + selectDeleteBase(xx,seq,1); + + /* + ** shift sequence right + */ + if (DBgetRelPos(xx,seq)==1) + for (i=1; iDB_gelCount && DBgetRelPos(xx,xx->DBorder[i+1])==1;i++); + else + i=0; + if (i==1) { + for (i=2; i <= xx->DB_gelCount; i++) { + DBsetRelPos(xx,xx->DBorder[i],DBgetRelPos(xx,xx->DBorder[i])-1); + } + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_REL_MODIFIED); + + } else { + DBsetRelPos(xx,seq,DBgetRelPos(xx,seq)+1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_REL_MODIFIED); + + for (i=1; seq != xx->DBorder[i] && i <= xx->DB_gelCount; i++); + for (j=i; j < xx->DB_gelCount && + DBgetRelPos(xx,xx->DBorder[j+1]) < DBgetRelPos(xx,seq) ; j++) + xx->DBorder[j] = xx->DBorder[j+1]; + xx->DBorder[j] = seq; + } + + + } else { + /* + ** get base at end of sequence + */ + char *s; + int l=DBgetLength(xx,seq); + s=DBgetSeq(xx,seq); + *ec++ = s[l-1]; + DBsetLength(xx,seq,l-1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_SEQ_MODIFIED|DB_FLAG_REL_MODIFIED); + selectDeleteBase(xx,seq,l); + } + + *eb='\0'; + *ib='\0'; + *ec='\0'; + + if (end_char_inserted) { + /* + ** Throw it away + */ + *extend_char = '\0'; + } else { + /* + ** complement if necessary + */ + if (use_tag != dir) { + int_f i=1; + sqcom_(extend_char,&i,(int_fl)1); + } + } + + /* + ** Insert unextend character on end of appropriate tag + */ + l = (int)(strlen(extend_char) + strlen(external_buff) + + strlen(internal_buff)); + if (l) { + char *newercomment; + newercomment = (char *) TAG_MALLOC( t->newcommentlen + l + 1); + if (use_tag==LEFT) { + /* add buffers to end of cutoff */ + strcpy(newercomment,t->newcomment); + strcat(newercomment,external_buff); + strcat(newercomment,extend_char); + strcat(newercomment,internal_buff); + } else { + /* add buffer to start of cutoff */ + strcpy(newercomment,internal_buff); + strcat(newercomment,extend_char); + strcat(newercomment,external_buff); + strcat(newercomment,t->newcomment); + } + TAG_FREE(t->newcomment); + t->newcomment = newercomment; + t->newcommentlen = (int)strlen(t->newcomment); + t->flags |= TAG_COMMENT_CHANGED; + } + + /* + ** Modify raw data stuff + */ + if (l) { + tagStruct *t; + char type[5]; + char name[FILE_NAME_LENGTH]; + int llen,lcut,wlen; + t = (tagStruct *) DBgetTags(xx,seq); + force_comment(t); + sscanf(t->newcomment,"%6d%6d%6d%*s",&llen,&lcut,&wlen); + strncpy(type,&t->newcomment[18],4); + strncpy(name,&t->newcomment[22],FILE_NAME_LENGTH); + wlen -= l; + if (use_tag==LEFT) lcut+=l; + sprintf(t->newcomment,"%6d%6d%6d",llen,lcut,wlen); + strncat(t->newcomment,type,4); + strncat(t->newcomment,name,FILE_NAME_LENGTH); + t->newcommentlen = (int)strlen(t->newcomment); + t->flags |= TAG_COMMENT_CHANGED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } + + if (dir == RIGHT) xx->cursorPos--; + + /* + ** Lengths are a changing + */ + calculateConsensusLength(xx); + + return 0; + +} + +int undo_unextend(EdStruct *xx, int seq, int dir, int time) +{ + + int c = (DBgetComp(xx,seq)==COMPLEMENTED); + char t_type[4]; + tagStruct *t; + int use_tag; + char extend_char; + int l; + int lextend; + int end_char_inserted = 0; + int added_internal = 0; + int added_external = 0; + + use_tag = (c && dir==LEFT || !c && dir==RIGHT )?RIGHT: LEFT; + strncpy(t_type, + (use_tag==RIGHT )?TAG_TYPE_RCUT:TAG_TYPE_LCUT,4); + + /* + ** Process tag information + ** collating *DE? flags and *INS flags + ** and adjusting other flags accordingly + */ + { + tagStruct *t; + int ipos; + + t = (tagStruct *) DBgetTags(xx,seq); + ipos = normalisePos(xx,seq,(dir==LEFT)?1:DBgetLength(xx,seq),1); + if (use_tag==RIGHT) ipos++; + + while (t != NULL) { + if ((t->time == time) && + strncmp(t->tagrec.type.c,TAG_TYPE_INSERT,3)==0) { + /* + ** Handle unextending past inserts + */ + end_char_inserted++; + extend_char = normaliseBase(xx,seq,t->tagrec.type.c[3]); + /* + extend_char = t->tagrec.type.c[3]; + */ + t->flags &= ~TAG_DELETED; + t->time = 0; + }else if ((t->time == time) && + strncmp(t->tagrec.type.c, TAG_TYPE_DELETE,3)==0) { + /* + ** Handle unextending past deletes + */ + if (use_tag==LEFT && t->tagrec.position == ipos || + use_tag==RIGHT && t->tagrec.position == (ipos+1)) { + added_external++; + } else { + added_internal++; + } + t->flags &= ~TAG_DELETED; + t->time = 0; + } else if (!(t->flags & TAG_DELETED) && + use_tag==LEFT && t->tagrec.position) { + /* + ** Handle all other positional adjustments + */ + t->tagrec.position++; + } + t = t->next; + } + } + + /* + ** Find appropriate tag, + ** create one if one doesn't exist + */ + t = force_get_tag(xx,seq,t_type); + + /* + ** No cutoff - ignore request + */ + l = t->newcommentlen; + if (! l) + return 1; + + /* + ** Determine character to extend + */ + lextend = added_internal+added_external+1-end_char_inserted; + if (use_tag==LEFT) { + /* Get character off end of cutoff */ + if (!end_char_inserted) { + extend_char = t->newcomment[l-1-added_internal]; + if (use_tag != dir) { + int_f i=1; + sqcom_(&extend_char,&i,(int_fl)1); + } + } + t->newcomment[l-lextend]='\0'; + t->newcommentlen = l-lextend; + } else { + /* Get character off start of cutoff */ + char *nc; + if (!end_char_inserted) { + extend_char = t->newcomment[added_internal]; + if (use_tag != dir) { + int_f i=1; + sqcom_(&extend_char,&i,(int_fl)1); + } + } + for (nc=t->newcomment;*nc=nc[lextend];nc++); + t->newcommentlen -= lextend; + } + + + + if (dir==LEFT){ + int i,j; + char *s; + + /* + ** insert base at start of sequence + */ + s=DBgetSeq(xx,seq); + for (j = DBgetLength(xx,seq)+1; j > 1; j--) + s[j-1] = s[j-2]; + s[0] = extend_char; + DBsetLength(xx,seq,DBgetLength(xx,seq)+1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_SEQ_MODIFIED|DB_FLAG_REL_MODIFIED); + selectInsertBase(xx,seq,1); + + /* + ** shift sequence left + */ + if (DBgetRelPos(xx,seq)==1) { + for (i=1; i <= xx->DB_gelCount; i++) { + if (seq == xx->DBorder[i]) { + for (j=i;j>1;j--) + xx->DBorder[j] = xx->DBorder[j-1]; + xx->DBorder[1] = seq; + } else + DBsetRelPos(xx,xx->DBorder[i],DBgetRelPos(xx,xx->DBorder[i])+1); + } + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_REL_MODIFIED); + + } else { + DBsetRelPos(xx,seq,DBgetRelPos(xx,seq)-1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_REL_MODIFIED); + + for (i=1; seq != xx->DBorder[i] && i <= xx->DB_gelCount; i++); + for (j=i; DBgetRelPos(xx,xx->DBorder[j-1]) > DBgetRelPos(xx,seq) ;j--) + xx->DBorder[j] = xx->DBorder[j-1]; + xx->DBorder[j] = seq; + } + + } else { + /* + ** insert base at end of sequence + */ + char *s; + int l=DBgetLength(xx,seq); + s=DBgetSeq(xx,seq); + s[l] = extend_char; + DBsetLength(xx,seq,l+1); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_SEQ_MODIFIED|DB_FLAG_REL_MODIFIED); + selectInsertBase(xx,seq,l+1); + } + + /* + ** Modify raw data stuff + */ + { + tagStruct *t; + char type[5]; + char name[FILE_NAME_LENGTH+1]; + int llen,lcut,wlen; + t = (tagStruct *) DBgetTags(xx,seq); + force_comment(t); + sscanf(t->newcomment,"%6d%6d%6d%*s",&llen,&lcut,&wlen); + strncpy(type,&t->newcomment[18],4); + strncpy(name,&t->newcomment[22],FILE_NAME_LENGTH); + wlen+=lextend; + if (use_tag==LEFT) lcut-=lextend; + sprintf(t->newcomment,"%6d%6d%6d",llen,lcut,wlen); + strncat(t->newcomment,type,4); + strncat(t->newcomment,name,FILE_NAME_LENGTH); + t->newcommentlen = (int)strlen(t->newcomment); + t->flags |= TAG_COMMENT_CHANGED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + + } + + if (dir == RIGHT) xx->cursorPos++; + + /* + ** Lengths are a changing + */ + calculateConsensusLength(xx); + + return 0; + +} + + +void meta_arrow (Widget widget, XEvent *event, int key) +/* +** Handle cut-off adjust +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(widget)); + int e_time = get_uniq_id(NULL); + int seq,pos; + int seq_length; + + seq = xx->cursorSeq; + pos = xx->cursorPos; + seq_length = DBgetLength(xx,seq); + + /* + ** determine which operation is to take place + */ + if (key==LEFT) { + if (seq) { + if (pos==1) { + if (! extend(xx,seq,LEFT,e_time)) { + recordEdit (&bucketStack, xx, undoExtend, seq, pos,LEFT,e_time); + redisplayWithCursor(xx); + } + } else if (pos>seq_length) { + if (! unextend(xx,seq,RIGHT,e_time)) { + recordEdit (&bucketStack, xx, undoUnextend, seq, pos, RIGHT, e_time); + redisplayWithCursor(xx); + } + } else + caretLeft(widget,event,NULL,0); + } else + caretLeft(widget,event,NULL,0); + } else { + if (seq) { + if (pos==1) { + if (! unextend(xx,seq,LEFT,e_time)) { + redisplayWithCursor(xx); + recordEdit (&bucketStack, xx, undoUnextend, seq, pos, LEFT,e_time); + } + } else if (pos>seq_length) { + if (! extend(xx,seq,RIGHT,e_time)) { + redisplayWithCursor(xx); + recordEdit (&bucketStack, xx, undoExtend, seq, pos, RIGHT, e_time); + } + } else + caretRight(widget,event,NULL,0); + } else + caretRight(widget,event,NULL,0); + } + +} + + + + + + +void meta_left (Widget widget, XEvent *event, String *params, + Cardinal *num_params) +{ + meta_arrow(widget,event,LEFT); +} + +void meta_right (Widget widget, XEvent *event, String *params, + Cardinal *num_params) +{ + meta_arrow(widget,event,RIGHT); +} + +void meta_up (Widget widget, XEvent *event, String *params, + Cardinal *num_params) +/* +** Handle cut-off adjust +*/ +{ + EdStruct *xx = widgetToEdStruct(XtParent(widget)); + + dump_tags(xx,xx->cursorSeq); + +} diff --git a/src/staden/extend.h b/src/staden/extend.h new file mode 100644 index 0000000..828a70f --- /dev/null +++ b/src/staden/extend.h @@ -0,0 +1,13 @@ +#ifndef _extend_h +#define _extend_h + +#include "edUtils.h" + +extern void meta_left (Widget widget, XEvent *event, String *, Cardinal *); +extern void meta_right (Widget widget, XEvent *event, String *, Cardinal *); +extern void meta_up (Widget widget, XEvent *event, String *, Cardinal *); +extern int extend(EdStruct *xx, int seq, int dir); +extern int unextend(EdStruct *xx, int seq, int dir); +extern int undo_unextend(EdStruct *xx, int seq, int dir, int time); + +#endif /* _extend_h */ diff --git a/src/staden/fmain.f b/src/staden/fmain.f new file mode 100644 index 0000000..561491a --- /dev/null +++ b/src/staden/fmain.f @@ -0,0 +1,3 @@ + EXTERNAL FMAIN + CALL FMAIN() + END diff --git a/src/staden/fort.h b/src/staden/fort.h new file mode 100644 index 0000000..67b4952 --- /dev/null +++ b/src/staden/fort.h @@ -0,0 +1,86 @@ +#ifndef _FORT_H +#define _FORT_H + +/* + * When passing INTEGERs from Fortran to C we need to know the type they'll be + * at the C end. We could define int_f as 'int *' (or 'long *') but this may + * cause obscureness and unreadability of C. + * + * Also we define the extra argument given to C by fortran for the length of + * an array passed. This may not necessarily be the same as int_f (either + * now or the future). So we define it as a separate type (int_fl). + */ + +typedef int int_f; +typedef int int_fl; + +/* + * Prototypes for Fortran functions. This helps to establish when we pass the + * wrong arguments to a function. + * + * Terminology: + * name_a : name is an array + * name_s : name is a string + * name_l : name is the length of items in array name_a or length of string + * name_s + * name : name is simple type + */ + +/* dbsyscommon.f */ +void readw_ (int_f *idevw, int_f *n, char *gel_a, int_f *maxgel, + int_fl gel_l); /* gel_l == *maxgel */ +void writew_(int_f *idevw, int_f *n, char *gel_a, int_f *maxgel, + int_fl gel_l); + +void readn_ (int_f *idevn, int_f *n, char *name_s, int_fl name_l); + /* name_l == DB_NAMELEN */ +void writen_(int_f *idevn, int_f *n, char *name_s, int_fl name_l); + +void readr_ (int_f *idev_r, int_f *n, int_f *relpg, int_f *lngthg, + int_f *lnbr, int_f *rnbr); +void writer_(int_f *idev_r, int_f *n, int_f *relpg, int_f *lngthg, + int_f *lnbr, int_f *rnbr); + +void pcon1_ (char *chr, int_f *chrsum); + +int_f indexs_(char *c, int_f *s); + +void munotp_(char *ret_s, int_fl ret_l, int_f *ip); + +void gtconc_(char *ret_s, int_fl rel_l, int_f *counts_a,int_f *idm, + float *cut/*, int_fl counts_l */); + +/* xsapConEdit.f */ +void dojoin_(int_f *relpg_a, int_f *lngthg_a,int_f *lnbr_a, int_f *rnbr_a, + int_f *ngels, int_f *nconts, int_f *lnconl, int_f *lnconr, + int_f *idbsiz, int_f *idevr, int_f *idevw, int_f *relx/*, + int_fl relpg_l, int_fl lngthg_l,int_fl lnbr_l, int_fl rnbr_l*/); + +/* subs89.f */ +void sqcom_ (char *seq_a, int_f *idim, int_fl seq_l); + +/* dbsysnew.f */ +void readtg_(int_f *idevt, int_f *i, int_f *lpos, int_f *llen, + int_f *lcom, int_f *ltype, int_f *next); + +void writtg_(int_f *idevt, int_f *i, int_f *lpos, int_f *llen, + int_f *lcom, int_f *ltype, int_f *next); + +void readcc_(int_f *idevc, int_f *i, int_f *icnt, int_f *next, + char *note_s, int_fl note_l); + +void writcc_(int_f *idevc, int_f *i, int_f *icnt, int_f *next, + char *note_s, int_fl note_l); + +void sqrev_ (char *seqnce_a,int_f *idim, int_fl seqnce_l); + +/* seeme.f */ +void flusho_(); + +/* fmain.f */ +void fmain_ (); + +/* xspec.f / nxspec.f */ +int_f xversn_(); + +#endif diff --git a/src/staden/fortran.h b/src/staden/fortran.h new file mode 100644 index 0000000..90d44ef --- /dev/null +++ b/src/staden/fortran.h @@ -0,0 +1,26 @@ +#ifndef _fortran_h +#define _fortran_h + +#include "fort.h" + +/* This defines the interfaces between fortran and c */ + +/* +** COMMON /DEVILS/ IDEVT,IDEVC,IDBSIZ,RELPG +*/ + +/* +** Map onto fortran common block holding RELPG array and IDEVRD +*/ +/* +** Snatch Fortran Common Block +** Don't EVER EVER let Rodger see this!! +*/ +extern struct { + int_f idevt; + int_f idevc; + int_f idbsiz; + int_f relpg[1000]; + } devils_; + +#endif /* _fortran_h */ diff --git a/src/staden/fxmain.f b/src/staden/fxmain.f new file mode 100644 index 0000000..46f7f93 --- /dev/null +++ b/src/staden/fxmain.f @@ -0,0 +1,4 @@ + CALL XCMAIN() +C call handle_unaligned_traps +C call print_unaligned_summary + END diff --git a/src/staden/gip.f b/src/staden/gip.f new file mode 100644 index 0000000..fa2c599 --- /dev/null +++ b/src/staden/gip.f @@ -0,0 +1,672 @@ +C GIP +C SCREEN OF 24 LINES, 80 CHARACTERS ASSUMED +C +C +C 14 May 1991 +C Modified to work on Sparcstations - connect digitiser to RS232-C port B +C 24 Sept 1992 +C digitizer is now specified by environment variable DIGITIZER +C + SUBROUTINE FMAIN() + PARAMETER (IDMENU=30) + INTEGER XLANE(4),DY,DXMIN,XPEN,YPEN,YMEAN + INTEGER XLMENU(IDMENU),XRMENU(IDMENU) + INTEGER YBMENU(IDMENU),YTMENU(IDMENU) + CHARACTER CHARS(IDMENU),GEL(512),BASE(4),MENUE,GELNAM*40,ANS + INTEGER MENUP(IDMENU),SEQROW,SEQCOL,SEQLEN + INTEGER DEVNOS(6) + INTEGER ESC + CHARACTER*40 FILDIG,FILVT + CHARACTER SPACE,CESC,CBELL +C ALL SCREEN OUTPUT CONTROLLED USING CURSOR +C SCREEN SIZE MAXROW, MAXCOL +C DIRECTION FROM HERE IS INC (1 OR -1) + INTEGER TITLER,TITLEC,CURER,CUREC,CURFR,CURFC + COMMON /ESCCHR/ CESC + COMMON /BELCHR/ CBELL + PARAMETER (ESC=27,IBELL=7) + PARAMETER (INC=1,MAXR=24) + PARAMETER (TITLER=1,TITLEC=20,LORDR=3,LORDC=20, + +IERRR=MAXR,IERRC=10, + +INSTR1=MAXR-INC,INSTC1=10,INSTR2=MAXR-2*INC,INSTC2=10, + +CURER=MAXR-4*INC,CUREC=1,CURFR=2,CURFC=20) + PARAMETER (SEQROW=6,SEQCOL=10,SEQLEN=60) +C PARAMETER (FILDIG='/dev/ttyb') + PARAMETER (FILDIG='DIGITIZER') + DATA XLMENU/0, + +0,200,400,600,0,200,400,600, + +0,200,400,600,0,200,400,600, + +0,200,400,600,0,200,400,600, + +0,400,0,0,0/ + DATA XRMENU/800, + +200,400,600,800,200,400,600,800, + +200,400,600,800,200,400,600,800, + +200,400,600,800,200,400,600,800, + +400,800,800,800,800/ + DATA YBMENU/000, + +1800,1800,1800,1800, + +1600,1600,1600,1600, + +1400,1400,1400,1400, + +1200,1200,1200,1200, + +1000,1000,1000,1000, + +800,800,800,800, + +600,600,400,200,0/ + DATA YTMENU/2000,2000,2000,2000,2000, + +1800,1800,1800,1800, + +1600,1600,1600,1600, + +1400,1400,1400,1400, + +1200,1200,1200,1200, + +1000,1000,1000,1000, + +800,800,600,400,200/ + DATA CHARS/'W', + +'T','C','G','A', + +'2','1','4','3', + +'V','D','H','B', + +'L','K','N','M', + +'R','Y','X','-', + +'5','6','7','8', + +'D','R','S','S','C'/ + DATA MENUP/0,1,2,3,4,5,6,7,8,9,10, + + 11,12,13,14,15,16,17,18,19,20, + + 21,22,23,24,50,51,53,52,56/ + DATA SPACE/' '/ + DATA BASE/'T','C','G','A'/ +C PROBLEM WITH DEFINING ESCAPE AND BELL SO DO IT HERE AND PUT IT COMMON +C WRITE(CESC,2000)ESC + CESC=CHAR(ESC) +2000 FORMAT(A1) + CBELL=CHAR(IBELL) + CALL UNITNO(KBIN,KBOUT,DEVNOS,6) +C OPEN LOGICAL UNIT FOR SOME TERMINAL OUTPUT + CALL OPENT(DEVNOS(6),FILVT) +C CLEAR SCREEN + CALL CLEAR +C WRITE TITLE + CALL CURSOR(TITLER,TITLEC) + CALL WRITES('GIP v1.0 Author: Rodger Staden') +C define allowed Y distance from current pen position + DY=80 + CALL OPENRS(DEVNOS(1),FILDIG,IOK,LRECL,8) + IF(IOK.NE.0)THEN + CALL CURSOR(IERRR,1) + CALL BLINE + CALL CURSOR(IERRR,IERRC) + CALL WRITES('******UNABLE TO OPEN DIGITIZER******') + STOP + END IF +C FLUSH DIGITIZER +C CALL FLUSH(DEVNOS(1)) + CALL CURSOR(INSTR1,1) + CALL BLINE + CALL CURSOR(INSTR1,INSTC1) + CALL WRITES('The film must be firmly fixed to the light box') +5 CONTINUE + CALL CURSOR(CURFR,1) + CALL BLINE + CALL CURSOR(CURFR,CURFC) + CALL WRITES('? File of file names=') + READ(KBIN,1000,ERR=5)GELNAM +1000 FORMAT(A) + CALL OPENRS(DEVNOS(3),GELNAM,IOK,LRECL,1) + IF(IOK.NE.0)THEN + CALL CURSOR(IERRR,1) + CALL BLINE + CALL CURSOR(IERRR,IERRC) + CALL WRITES('******ERROR OPENING FILE******') + GO TO 5 + END IF +C CLEAR ERROR LINE IN CASE ITS BEEN USED + CALL CURSOR(IERRR,1) + CALL BLINE + CALL CURSOR(INSTR2,1) + CALL BLINE + CALL CURSOR(INSTR2,INSTC2) + CALL WRITES( + +'Hit device menu origin, program origin, then hit start') +90 CONTINUE + CALL READPN(XPEN,YPEN,1,DEVNOS(1),KBOUT) +C START? + CALL INTERP(XLMENU,XRMENU,YBMENU,YTMENU,MENUP,CHARS, + +IDMENU,XPEN,YPEN,MENU,MENUE,KBOUT) + IF(MENU.NE.52)GO TO 90 +100 CONTINUE +C CLEAR ERROR LINE IN CASE ITS BEEN USED + CALL CURSOR(IERRR,1) + CALL BLINE +C GET LANE ORDER +120 CONTINUE +C GET LANE ORDER + CALL LORDER(XLMENU,XRMENU,YBMENU,YTMENU,MENUP, + +CHARS,MENUE,IDMENU,BASE,DEVNOS(1),KBOUT,INSTR2,INSTC2, + +LORDR,LORDC) +C CLEAR ERROR LINE IN CASE ITS BEEN USED + CALL CURSOR(IERRR,1) + CALL BLINE +C GET LANE COORDS + CALL GETLAN(XLANE,YMEAN,DXMIN, + +XLMENU,XRMENU,YBMENU,YTMENU,MENUP,CHARS,MENUE,IDMENU, + +DEVNOS(1),KBOUT,INSTR2,INSTC2) +C CLEAR ERROR LINE IN CASE ITS BEEN USED + CALL CURSOR(IERRR,1) + CALL BLINE +C POINT TO GEL + IPGEL=0 +****************************************************** +200 CONTINUE + CALL CURSOR(INSTR2,1) + CALL BLINE + CALL CURSOR(INSTR2,INSTC2) + CALL WRITES('Hit start when ready to begin reading') + CALL READPN(XPEN,YPEN,1,DEVNOS(1),KBOUT) +C START? + CALL INTERP(XLMENU,XRMENU,YBMENU,YTMENU,MENUP,CHARS, + +IDMENU,XPEN,YPEN,MENU,MENUE,KBOUT) + IF(MENU.EQ.52)THEN + CALL CURSOR(INSTR2,1) + CALL BLINE + CALL CURSOR(INSTR2,INSTC2) + CALL WRITES + + ('Hit bands, uncertainty codes, DELETE, RESET or STOP') + CALL READG( + + XLMENU,XRMENU,YBMENU,YTMENU,MENUP,CHARS, + + IDMENU,XLANE,YMEAN,DY,DXMIN,BASE,GEL,IPGEL, + + SEQROW,SEQCOL,SEQLEN,DEVNOS(1),KBOUT,INSTR2,INSTC2) + CALL CURSOR(INSTR2,INSTC2) + IF(IPGEL.GT.0)THEN +50 CONTINUE + CALL CURSOR(INSTR2,1) + CALL BLINE + CALL CURSOR(INSTR2,INSTC2) + CALL WRITES('File name for this gel reading=') + READ(KBIN,1000,ERR=50)GELNAM + CALL OPENRS(DEVNOS(5),GELNAM,IOK,LRECL,1) + IF(IOK.EQ.0)THEN + CALL FMTDK(DEVNOS(5),GEL,IPGEL) + CLOSE(UNIT=DEVNOS(5)) + WRITE(DEVNOS(3),1008)GELNAM +1008 FORMAT(A) + CALL CURSOR(INSTR2,INSTC2) + ELSE + CALL CURSOR(IERRR,1) + CALL BLINE + CALL CURSOR(IERRR,IERRC) + CALL WRITES('******ERROR OPENING FILE******') + GO TO 50 + END IF +C CLEAR GEL FROM SCREEN + DO 441 I=1,IPGEL + CALL WRITEG(' ',SEQCOL,SEQROW,SEQLEN,I) +441 CONTINUE + END IF + CALL CURSOR(INSTR2,1) + CALL BLINE + CALL CURSOR(INSTR2,INSTC2) + CALL WRITES('? (y/n) (y) Read another sequence ') + READ(KBIN,1010)ANS +1010 FORMAT(A1) + IF((ANS.EQ.' ').OR.(ANS.EQ.'Y').OR.(ANS.EQ.'y'))GO TO 100 + CALL CLEAR + STOP + END IF + GO TO 200 + END +C DIGIT +C AUTHOR RODGER STADEN +C ROUTINES USED BY GELIN +C NONSTANDARD: CARRIAGE CONTROL $ USED BY ROUTINE BELL +C ROUTINES IN THIS LIB: +C READG +C INOUT +C LANE +C GETLAN +C GETMEN +C LORDER +C WRITEG +C INTERP +C READPN + SUBROUTINE READG(XLMENU,XRMENU,YBMENU,YTMENU,MENUP,CHARS, + +IDMENU,XLANE,YMEAN,DY,DXMIN,BASE,GEL,IPGEL,SEQROW,SEQCOL, + +SEQLEN,IDEVD,KBOUT,INSTR2,INSTC2) +C AUTHOR RODGER STADEN +C READS A SEQUENCE USING THE DIGITIZER UNTIL A STOP COMMAND IS GIVEN +C LANE COORDINATES CAN BE RESET. + INTEGER XLANE(4),DY,DXMIN,XPEN,YPEN,YMEAN + INTEGER XLMENU(IDMENU),XRMENU(IDMENU) + INTEGER YBMENU(IDMENU),YTMENU(IDMENU) + CHARACTER CHARS(IDMENU),GEL(512),BASE(4),MENUE,LASTCH + INTEGER MENUP(100),SEQCOL,SEQROW,SEQLEN,INSTR2,INSTC2 + EXTERNAL LANE + LASTCH=' ' +300 CONTINUE + CALL READPN(XPEN,YPEN,0,IDEVD,KBOUT) +C IN LANES? +C***************************************************** + L=LANE(XLANE,YMEAN,DY,DXMIN,XPEN,YPEN) + IF(L.NE.0)THEN + CALL GBELL(1,KBOUT) +C IN LANES +C EXPECTING A BAND SO DEAL WITH IT +C INCREMENT POINTER TO GEL CHARACTERS + IPGEL=IPGEL+1 + GEL(IPGEL)=BASE(L) +C PUT ON SCREEN ETC + CALL WRITEG(GEL(IPGEL),SEQCOL,SEQROW,SEQLEN,IPGEL) + LASTCH='C' + ELSE +C IN MENU? + CALL INTERP(XLMENU,XRMENU,YBMENU,YTMENU,MENUP,CHARS, + + IDMENU,XPEN,YPEN,MENU,MENUE,KBOUT) +C UNCERTAINTY CODE? + IF((MENU.GT.0).AND.(MENU.LT.25))THEN +C UNCERTAINTY CODE + CALL GBELL(1,KBOUT) + IPGEL=IPGEL+1 + GEL(IPGEL)=MENUE + LASTCH='C' + CALL WRITEG(GEL(IPGEL),SEQCOL,SEQROW,SEQLEN,IPGEL) +C DELETE? + ELSE IF(MENU.EQ.50)THEN +C DELETE +C IF LAST CHAR A BASE THEN NEED TO REMOVE IT FROM GEL + IF((LASTCH.EQ.'C').AND.(IPGEL.GT.0))THEN + CALL GBELL(1,KBOUT) + CALL WRITEG(' ',SEQCOL,SEQROW,SEQLEN,IPGEL) + IPGEL=IPGEL-1 + END IF +C RESET LANE CENTRES? + ELSE IF(MENU.EQ.51)THEN + CALL GBELL(1,KBOUT) + CALL GETLAN(XLANE,YMEAN,DXMIN, + + XLMENU,XRMENU,YBMENU,YTMENU, + + MENUP,CHARS,MENUE,IDMENU, + + IDEVD,KBOUT,INSTR2,INSTC2) + CALL CURSOR(INSTR2,1) + CALL BLINE + CALL CURSOR(INSTR2,INSTC2) + CALL WRITES + + ('Hit bands, uncertainty codes, DELETE, RESET or STOP') +C STOP? + ELSE IF(MENU.EQ.53)THEN + CALL GBELL(1,KBOUT) + RETURN +C FOR NOW NOT FOUND SO IGNORE + END IF + END IF +C NOT IN MENU +C NOT IN MENU OR LANES SO IGNORE + GO TO 300 + END +C INOUT +C +C FUNCTION TO RETURN VALUE 1 IF XPEN,YPEN IN BOX, 0 ELSE +C AUTHOR RODGER STADEN + INTEGER FUNCTION INOUT(X1,X2,Y1,Y2,XPEN,YPEN) + IMPLICIT INTEGER (A-Z) + INOUT=0 + IF((XPEN.LT.X1).OR.(XPEN.GT.X2))RETURN + IF((YPEN.LT.Y1).OR.(YPEN.GT.Y2))RETURN + INOUT=1 + RETURN + END +C LANEORDER +C GETS LANE ORDER FROM TABLET + SUBROUTINE LORDER(XLMENU,XRMENU,YBMENU,YTMENU,MENUP, + +CHARS,MENUE,IDMENU,BASE,IDEVD,KBOUT,INSTR2,INSTC2, + +LORDR,LORDC) +C AUTHOR RODGER STADEN + INTEGER XLMENU(IDMENU),XRMENU(IDMENU) + INTEGER YBMENU(IDMENU),YTMENU(IDMENU) + INTEGER MENUP(IDMENU),INSTR2,INSTC2 + CHARACTER BASE(4),BASES(4),CHARS(IDMENU),MENUE,SPACE + CHARACTER BASET*4 + PARAMETER (IERRR=24,IERRC=10) + SAVE BASES,SPACE + DATA SPACE/' '/ + DATA BASES/'T','C','G','A'/ +C +10 CONTINUE + DO 5 I=1,4 + BASET(I:I)=BASE(I) +5 CONTINUE +C + CALL CURSOR(LORDR,1) + CALL BLINE + CALL CURSOR(LORDR,LORDC) + CALL WRITES('Lane order is ') + CALL WRITES(BASET) + CALL CURSOR(INSTR2,1) + CALL BLINE + CALL CURSOR(INSTR2,INSTC2) + CALL WRITES('If lane order correct hit CONFIRM, else hit RESET') + CALL READPN(XPEN,YPEN,1,IDEVD,KBOUT) + CALL INTERP(XLMENU,XRMENU,YBMENU,YTMENU,MENUP,CHARS, + +IDMENU,XPEN,YPEN,MENU,MENUE,KBOUT) + IF(MENU.EQ.56)THEN +C CLEAR ERROR LINE IN CASE ITS BEEN USED + CALL CURSOR(IERRR,1) + CALL BLINE + RETURN + END IF + IF(MENU.EQ.51)THEN + CALL CURSOR(INSTR2,1) + CALL BLINE + CALL CURSOR(INSTR2,INSTC2) + CALL WRITES('Define lane order, left to right, using menu') +C COUNT POINTS + IP=1 +20 CONTINUE + IF(IP.LE.4)THEN + CALL READPN(XPEN,YPEN,1,IDEVD,KBOUT) + CALL INTERP(XLMENU,XRMENU,YBMENU,YTMENU,MENUP, + + CHARS,IDMENU,XPEN,YPEN,MENU,MENUE,KBOUT) + DO 30 I=1,4 + IF(MENUE.EQ.BASES(I))THEN + BASE(IP)=MENUE + GO TO 31 + END IF +30 CONTINUE +31 CONTINUE + IP=IP+1 + GO TO 20 + END IF +C ALL DIFFERENT? + DO 40 I=1,4 + DO 41 J=I+1,4 + IF(BASE(I).EQ.BASE(J))THEN +C SAME! + CALL CURSOR(INSTR2,1) + CALL BLINE + CALL CURSOR(IERRR,IERRC) + CALL WRITES('*****DUPLICATION IN LANE ORDER*****') + DO 35 K=1,4 + BASE(K)=BASET(K:K) +35 CONTINUE + GO TO 10 + END IF +41 CONTINUE +40 CONTINUE + END IF + GO TO 10 + END +C INTERPXY +C +C INTERPRETS X,Y COORDS FROM DIGITIZER + SUBROUTINE INTERP(XL,XR,YB,YT,MENUP,CHARS, + +IDMENU,XPEN,YPEN,MENU,MENUE,KBOUT) +C AUTHOR RODGER STADEN + INTEGER XL(IDMENU),XR(IDMENU),YB(IDMENU),YT(IDMENU) + INTEGER MENUP(IDMENU),XPEN,YPEN + CHARACTER CHARS(IDMENU),MENUE + EXTERNAL INOUT + PARAMETER (IERRR=24,IERRC=10) + MENU=0 + MENUE=CHARS(1) +C IN MENU AT ALL? + IF(INOUT(XL(1),XR(1),YB(1),YT(1),XPEN,YPEN).EQ.0)RETURN + DO 100 I=2,IDMENU + IF(INOUT(XL(I),XR(I),YB(I),YT(I),XPEN,YPEN).NE.1)GO TO 100 +C MATCH + MENU=MENUP(I) + MENUE=CHARS(I) + RETURN +100 CONTINUE +C ERROR IN MENU + MENU=0 + MENUE=CHARS(1) + CALL CURSOR(IERRR,1) + CALL BLINE + CALL CURSOR(IERRR,IERRC) + CALL WRITES('******ERROR IN MENU******') + RETURN + END +C +C READPEN GETS COORDS FROM DIGITIZER + SUBROUTINE READPN(XPEN,YPEN,IBELL,IDEVD,KBOUT) +C AUTHOR RODGER STADEN + CHARACTER INPUT*11,INPUTX*5,INPUTY*5 + INTEGER XPEN,YPEN +C EQUIVALENCE (INPUT(2:6),INPUTX),(INPUT(7:11),INPUTY) +C NOTE ON IBM NEXT LINE EQUIVALENCE USED + EQUIVALENCE (INPUT(1:5),INPUTX),(INPUT(6:10),INPUTY) + PARAMETER (IERRR=24,IERRC=10) +1000 FORMAT(A) +1 CONTINUE + READ(IDEVD,1000,ERR=1)INPUT +1002 FORMAT(I5) + READ(INPUTX,1002,ERR=100)XPEN + READ(INPUTY,1002,ERR=100)YPEN + IF(IBELL.EQ.1)CALL GBELL(1,KBOUT) +C READ 2nd NEWLINE ON SUN + READ(IDEVD,1000,ERR=1)INPUT + RETURN +100 CONTINUE + CALL CURSOR(IERRR,1) + CALL BLINE + CALL CURSOR(IERRR,IERRC) + CALL WRITES('*********ERROR READING PEN********') + RETURN + END +C WRITEGEL + SUBROUTINE WRITEG(CHAR,STARTX,STARTY,LENGTH,IPGEL) +C AUTHOR RODGER STADEN +C WRITE OUT A CHARACTER + CHARACTER CHAR + INTEGER STARTX,STARTY,ROW,COL + ROW=(IPGEL-1)/LENGTH + COL=MOD(IPGEL,LENGTH) + IF(COL.EQ.0)COL=LENGTH + ROW=STARTY+ROW + COL=STARTX+COL + CALL CURSOR(ROW,COL) + CALL WRITES(CHAR) + RETURN + END +C +C GETLANE + SUBROUTINE GETLAN(XLANE,YMEAN,DXMIN, + +XLMENU,XRMENU,YBMENU,YTMENU,MENUP,CHARS,MENUE,IDMENU, + +IDEVD,KBOUT,INSTR2,INSTC2) +C AUTHOR RODGER STADEN +C GETS LANE ORDER + INTEGER XLANE(4) + INTEGER YTEMP(4) + INTEGER XLMENU(IDMENU),XRMENU(IDMENU) + INTEGER YBMENU(IDMENU),YTMENU(IDMENU) + INTEGER MENUP(IDMENU),INSTR2,INSTC2 + INTEGER XPEN,YPEN,YMEAN,DXMIN + CHARACTER CHARS(IDMENU),MENUE,SEPAR*10 + PARAMETER (IERRR=24,IERRC=10) + INSTR3=4 + INSTC3=20 +100 CONTINUE + CALL CURSOR(INSTR2,1) + CALL BLINE + CALL CURSOR(INSTR2,INSTC2) + CALL WRITES + +('Hit START, then, left to right,') + CALL WRITES(' the start centres of this clones lanes') + CALL READPN(XPEN,YPEN,1,IDEVD,KBOUT) + CALL INTERP(XLMENU,XRMENU,YBMENU,YTMENU,MENUP,CHARS, + +IDMENU,XPEN,YPEN,MENU,MENUE,KBOUT) +C START? + IF(MENU.NE.52)GO TO 100 +C GOT START, EXPECT LANES NOW +200 CONTINUE + DO 300 I=1,4 + CALL READPN(XPEN,YPEN,1,IDEVD,KBOUT) +C IN MENU? + CALL INTERP(XLMENU,XRMENU,YBMENU,YTMENU,MENUP,CHARS, + +IDMENU,XPEN,YPEN,MENU,MENUE,KBOUT) + IF(MENU.NE.0)GO TO 100 +C NOT IN MENU SO RECORD POSITION + XLANE(I)=XPEN + YTEMP(I)=YPEN +300 CONTINUE + YMEAN=(YTEMP(1)+YTEMP(2)+YTEMP(3)+YTEMP(4))/4 +C FIND LANE SEPARATION + DXMIN=(XLANE(4)-XLANE(1))/3 + WRITE(SEPAR,1011)DXMIN + CALL CURSOR(INSTR3,1) + CALL BLINE + CALL CURSOR(INSTR3,INSTC3) + CALL WRITES('Mean lane separation = ') + CALL WRITES(SEPAR) + CALL CURSOR(INSTR2,1) + CALL BLINE + CALL CURSOR(INSTR2,INSTC2) + CALL WRITES( + +'If separation ok hit CONFIRM, else hit RESET') +1011 FORMAT(I6) + CALL READPN(XPEN,YPEN,1,IDEVD,KBOUT) +C IN MENU? + CALL INTERP(XLMENU,XRMENU,YBMENU,YTMENU,MENUP,CHARS, + +IDMENU,XPEN,YPEN,MENU,MENUE,KBOUT) +C + IF(MENU.NE.56)GO TO 100 +C SET MINIMUM LANE CENTRE CLOSENESS TO 50% OF SEPARATION + DXMIN=DXMIN/2 +C CLEAR ERROR LINE IN CASE ITS BEEN USED + CALL CURSOR(IERRR,1) + CALL BLINE + RETURN + END +C LANE +C RETURNS THE LANE NUMBER FOR GELIN + INTEGER FUNCTION LANE(XLANE,YMEAN,DY,DXMIN,XPEN,YPEN) +C AUTHOR RODGER STADEN + INTEGER XLANE(4),YMEAN,D(4) + INTEGER XPEN,YPEN,DXMIN,DY,X1,X2,Y1,Y2,DMIN + EXTERNAL INOUT + LANE=0 +C IS THE POINT IN THE LANES? + X1=XLANE(1)-DXMIN + X2=XLANE(4)+DXMIN + Y1=YMEAN-DY + Y2=YMEAN+DY + IF(INOUT(X1,X2,Y1,Y2,XPEN,YPEN).EQ.0)RETURN +C +C IN LANES +C WHICH IS NEAREST XLANE VALUE + DO 10 I=1,4 + D(I)=ABS(XLANE(I)-XPEN) +10 CONTINUE +C WHICH IS LEAST? + DMIN=D(1) + ICLOSE=1 + DO 20 I=2,4 + IF(D(I).GT.DMIN)GO TO 20 +C CLOSER + ICLOSE=I + DMIN=D(I) +20 CONTINUE +C CLOSEST TO ICLOSE SO SET THIS CHAR AND SHIFT THIS LANE CENTRE + XLANE(ICLOSE)=XPEN + YMEAN=YPEN + LANE=ICLOSE +C MAKE SURE LANES ARE NOT TOO CLOSE + DO 30 I=2,4 + IF(ABS(XLANE(I-1)-XLANE(I)).LT.DXMIN)LANE=0 +30 CONTINUE +C RESET LANE SEPARATION + DXMIN=(XLANE(4)-XLANE(1))/6 + RETURN + END +C BELL +C SUBROUTINE TO RING BELL N TIMES + SUBROUTINE GBELL(N,KBOUT) +C AUTHOR RODGER STADEN + CHARACTER CBELL + COMMON /BELCHR/ CBELL + CALL WRITES(CBELL) + RETURN + END + SUBROUTINE OPENT(IDEV,FILNAM) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*) + COMMON /TERM/IDEVT + SAVE /TERM/ + PARAMETER (IERRR=24,IERRC=10) + IDEVT=IDEV + CALL OPENRS(IDEV,FILNAM,IOK,LRECL,10) + IF(IOK.EQ.0)RETURN + CALL CURSOR(IERRR,1) + CALL BLINE + CALL CURSOR(IERRR,IERRC) + CALL WRITES('****ERROR OPENING SCREEN FOR OUTPUT****') + RETURN + END + SUBROUTINE CLEAR +C AUTHOR: RODGER STADEN + CHARACTER STRING*4,CESC + COMMON /ESCCHR/ CESC + DATA STRING(2:4)/'[2J'/ + STRING(1:1)=CESC +C CLEAR SCREEN + CALL WRITES(STRING) + RETURN + END + SUBROUTINE WRITES(CHARS) +C AUTHOR: RODGER STADEN + CHARACTER CHARS*(*) + INTEGER NUL + COMMON /TERM/IDEVT + SAVE /TERM/ + DATA NUL/0/ +C WRITE(IDEVT,1000)NUL,CHARS +C1000 FORMAT(A1,A) + WRITE(IDEVT,1000)CHARS +1000 FORMAT(A,$) + CALL FLUSH(IDEVT) + RETURN + END + SUBROUTINE CURSOR(LINE,COLUMN) +C AUTHOR: RODGER STADEN +C SETS CURSOR TO LINE AND COLUMN + INTEGER COLUMN + CHARACTER BLIN*2,BCOL*2,SPACE,ZERO,STRING*8,CESC + COMMON /ESCCHR/ CESC + EQUIVALENCE (STRING(3:4),BLIN),(STRING(6:7),BCOL) + SAVE STRING,ZERO,SPACE + DATA STRING(2:2)/'['/,STRING(8:8)/'H'/ + DATA STRING(5:5)/';'/ + DATA SPACE/' '/,ZERO/'0'/ + STRING(1:1)=CESC + WRITE(BCOL,1002,ERR=100)COLUMN + WRITE(BLIN,1002,ERR=100)LINE +1002 FORMAT(I2.2) +C NEED TO SET SPACES TO ZEROS +C IF(BCOL(1:1).EQ.SPACE)BCOL(1:1)=ZERO +C IF(BLIN(1:1).EQ.SPACE)BLIN(1:1)=ZERO + CALL WRITES(STRING) + RETURN +100 CONTINUE + WRITE(*,*)'ERROR IN CURSOR SUBROUTINE' + RETURN + END + SUBROUTINE BLINE + CHARACTER BLANK*79 + SAVE BLANK + DATA BLANK/' '/ + CALL WRITES(BLANK) + RETURN + END + SUBROUTINE FMTDK(IDEV,SEQNCE,IDIM) +C AUTHOR: RODGER STADEN + CHARACTER SEQNCE(IDIM) +C SET POINTERS TO FIRST AND LAST ELEMENTS ONE WRITE + JS=1 + JE=60 +10 CONTINUE +C SET JE TO LAST ELEMENT IF NECESSARY + IF(JE.GT.IDIM)JE=IDIM + WRITE(IDEV,1002)(SEQNCE(I),I=JS,JE) +1002 FORMAT(' ',60A1) +C TEST FOR END + IF(JE.EQ.IDIM)GO TO 20 +C INCREMENT FIRST AND LAST POINTERS + JS=JE+1 + JE=JE+60 + GO TO 10 +20 CONTINUE + RETURN + END diff --git a/src/staden/help.c b/src/staden/help.c new file mode 100644 index 0000000..2041c67 --- /dev/null +++ b/src/staden/help.c @@ -0,0 +1,305 @@ +/* + Title: help + + File: help.c + Purpose: Routines to provide a help service + Last update: Wed Jun 20 1990 +*/ + + +/* + This module provides a help service, including an independent + help widget. +*/ + + + + +/* ---- Includes ---- */ + +#include "help.h" +#include "FtoC.h" /* IMPORT: Fstr2Cstr */ +#include "progSpec.h" /* IMPORT: botHelpOpt, topHelpOpt, + helpTextFN, helpPtrsFN */ + +#include /* IMPORT: stderr, fprintf, fscanf, fseek, + fclose, EOF */ +#include "mystdlib.h" /* IMPORT: calloc */ +/*#include */ /* IMPORT: strcat, strcpy */ +#include "values.h" /* IMPORT: MAXLONG */ +#include "fort.h" +#include "helpnmenu.h" /* IMPORT: createmenu, optTransTab, helptopics */ + +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include + +/* ---- Constants ---- */ + +#define FORTRANRecLen 80 + +/* ---- Global variables ---- */ + +extern Dimension dialogue_width; +extern Dimension dialogue_height; + +/* ---- Static variables ---- */ + +static Widget helpShellWid; +static Widget labelWid; /* Which says `Currently displaying: ...' */ +static Widget formWid; /* Which holds either ... */ +static Widget panedWid; /* Which holds either ... */ +static Widget listWid; /* ... the list of topics or ... */ +static Widget listWid1; /* ... the list of topics or ... */ +static Widget textWid; /* ... which holds help on the current topic */ +static Widget topicsButton; /* 'Display/Remove topics' */ +static Boolean helpShellMapped = False; + +#define labelLen 256 +static char label[labelLen]; + +static void DisplayTopic(int index) +/* + Fill the text widget with help on the selected option. + Ensure the text widget is displayed. +*/ +{ + int i; + XawTextBlock textBlock; + char *cptr; + + /* Clear the text widget */ + textBlock.firstPos=0; + textBlock.length=0; + textBlock.ptr=0; + textBlock.format=FMT8BIT; + XawTextReplace(textWid, (long) 0, MAXLONG, &textBlock); + + /* insert the lines of text into the text widget */ + while (cptr = gethelp2(index)) { + textBlock.ptr=cptr; + textBlock.length=strlen(cptr); + XawTextReplace(textWid, MAXLONG, MAXLONG, &textBlock); + } + + strcpy(label, "Currently displaying: "); + strcat(label, helpindex[index].name); + + XtVaSetValues(labelWid, XtNlabel, label, NULL); + + /* Ensure correct topic is highlighted */ + for (i = 0; ilist_index]); +} + +static void topicsCallback(Widget w,XtPointer client_data,XtPointer call_data) +{ + static int state = 0; + + if (state ^= 1) { + XtUnmanageChild(listWid1); + XtVaSetValues(topicsButton, XtNlabel, "Display topics", NULL); + } else { + /* Make sure we have things in the correct order. */ + XtUnmanageChild(textWid); + XtVaSetValues(listWid1, XtNpreferredPaneSize, 100, NULL); + XtManageChild(listWid1); + XtManageChild(textWid); + XtVaSetValues(topicsButton, XtNlabel, "Remove topics", NULL); + } +} + +/* ---- Exported routines ---- */ + +Widget CreateHelpShell(Widget toplevelWid) +/* + Create the help shell as a child of `toplevelWid', initially with + the list of topics available. + The shell is not initially mapped. +*/ +{ Widget removeButton; + + /* + The toplevel shell widget holds a form widget + */ + helpShellWid = XtVaCreatePopupShell("Help", topLevelShellWidgetClass, + toplevelWid, NULL); + + XtVaSetValues(helpShellWid, + XtNwidth, dialogue_width, + XtNminWidth, dialogue_width, + XtNheight, dialogue_height * 4/3, + XtNminHeight, dialogue_height * 4/3, NULL); + + formWid = XtVaCreateManagedWidget("helpForm", formWidgetClass, + helpShellWid, + XtNresizable, True, + NULL); + + + /* + The form widget holds a label and two button ... + */ + strcpy(label, "Currently displaying: "); + if (helpindex[0].name) + strcat(label, helpindex[0].name); + + labelWid = XtVaCreateManagedWidget("currDisp", labelWidgetClass, formWid, + XtNlabel, label, + XtNfromVert, labelWid, + NULL); + + removeButton = XtVaCreateManagedWidget("rmHelp", commandWidgetClass, + formWid, + XtNfromVert, labelWid, + NULL); + XtAddCallback(removeButton, XtNcallback, RemoveCallback, NULL); + + topicsButton = XtVaCreateManagedWidget("topipButton", commandWidgetClass, + formWid, + XtNlabel, "Remove topics", + XtNfromVert, labelWid, + XtNfromHoriz, removeButton, + XtNresize, True, + NULL); + XtAddCallback(topicsButton, XtNcallback, topicsCallback, NULL); + + /* + * Create a form widget specifically to hold only the text widget + * or the list widget & viewport. + */ + panedWid = XtVaCreateManagedWidget("helpPane", panedWidgetClass, + formWid, + XtNfromVert, removeButton, + XtNtop, XtChainTop, + XtNbottom, XtChainBottom, + XtNleft, XtChainLeft, + XtNright, XtChainRight, + NULL); + + /* + The pane widget also holds (togglable) + (a) a list widget and a viewport.... + */ + listWid1 = XtVaCreateManagedWidget("topicsViewport", viewportWidgetClass, + panedWid, + XtNfromVert, removeButton, + XtNallowVert, True, + XtNforceBars, True, + XtNpreferredPaneSize, dialogue_height/2, + NULL); + + listWid = XtVaCreateManagedWidget("topicsList", listWidgetClass, + listWid1, + XtNlist, helptopics, + NULL); + XtAddCallback(listWid, XtNcallback, ListCallback, NULL); + + /* + ... and a ... + (b) a text widget + */ + textWid = XtVaCreateManagedWidget("text", asciiTextWidgetClass, panedWid, + XtNeditType, XawtextEdit, + XtNscrollVertical, XawtextScrollAlways, + NULL); + + return(helpShellWid); +} + + + + +void help(int topic) +/* + Ensure the help shell is displayed, with ``topic'' selected. +*/ +{ if (!helpShellMapped) + { XtPopup(helpShellWid, XtGrabNone); + helpShellMapped = True; + } + + DisplayTopic(topic); +} + + + + +void help_x(int_f *HELPS_p, + int_f *HELPE_p, + int_f *BOTOPT_p, + int_f *TOPOPT_p, + char *HELPF_p, + int_f *IDEV_p, + int_f *KBIN_p, + int_f *KBOUT_p, + int_fl HELPF_l) +/* + This function does nothing +*/ +{ +} + + + + +void help2_x(int_f *HELPS_p, int_f *HELPE_p, char *HELPF_p, + int_f *IDEV_p, + int_f *KBIN_p, int_f *KBOUT_p, + int_fl HELPF_l) +/* + This function does nothing +*/ +{ +} + + + + +void sethelp_x(int_f HELPS[], /* Array of start record numbers */ + int_f HELPE[], /* Array of end record numbers */ + int_f *BOTOPT_p, /* Lower bound */ + int_f *TOPOPT_p, /* Upper bound */ + char *POINTF_p, /* Name of help record pointer file */ + int_f *IDEV_p, + int_f *KBOUT, + int_fl POINTF_l) +/* + This function does nothing +*/ +{ +} + + + + + + + diff --git a/src/staden/help.h b/src/staden/help.h new file mode 100644 index 0000000..f5762d3 --- /dev/null +++ b/src/staden/help.h @@ -0,0 +1,93 @@ +/* + Title: help + + File: help.h + Purpose: Routines to provide a help service + Last update: Mon May 12 1990 +*/ + + +/* + This module provides a help service, including an independent + help widget. +*/ + + +/* + C (internal) - SUN FORTRAN (external) name conversions + This table is for the compiler specifically mentioned above, so + as well as being hostage to its horrors we can make use of its + advantages, such as long identifiers. +*/ +#define help_x help_ +#define help2_x help2_ +#define sethelp_x sethlp_ + + + +/* ---- Includes ---- */ + +#include "fort.h" +#include /* IMPORT: Widget */ + + + + +/* ---- Exports ---- */ + + +extern Widget CreateHelpShell(Widget toplevelWid); +/* + Create the help shell as a child of `toplevelWid', initially with + the list of topics available. + The shell is not initially mapped. +*/ + + + + +extern void help(int topic); +/* + Ensure the help shell is displayed, with ``topic'' selected. +*/ + + + + +extern void help_x(int_f *HELPS_p, int_f *HELPE_p, + int_f *BOTOPT, int_f *TOPOPT, + char *HELPF_p, + int_f *IDEV_p, + int_f *KBIN_p, int_f *KBOUT_p, + int_fl HELPF_l); +/* + This function does nothing +*/ + + + + +extern void help2_x(int_f *HELPS_p, int_f *HELPE_p, char *HELPF_p, + int_f *IDEV_p, + int_f *KBIN_p, int_f *KBOUT_p, + int_fl HELPF_l); +/* + This function does nothing +*/ + + + + + +extern void sethelp_x(int_f HELPS[], /* Array of start record numbers */ + int_f HELPE[], /* Array of end record numbers */ + int_f *BOTOPT_p, /* Lower bound */ + int_f *TOPOPT_p, /* Upper bound */ + char *POINTF_p, /* Name of help record pointer file */ + int_f *IDEV_p, + int_f *KBOUT, + int_fl POINTF_l); +/* + This function does nothing +*/ + diff --git a/src/staden/helpnmenu.c b/src/staden/helpnmenu.c new file mode 100644 index 0000000..5ddf434 --- /dev/null +++ b/src/staden/helpnmenu.c @@ -0,0 +1,285 @@ +/*--------------------------------------------------------------------------*\ +|* *| +|* Help and menu routines. *| +|* *| +\*--------------------------------------------------------------------------*/ + +#include +#include +#include +#include +#include +#include +#include /* IMPORT: strdup (hopefully!) */ +#include "mcspec.h" /* IMPORT: getenv */ +#include "FtoC.h" /* IMPORT: Fstr2Cstr */ +#include "helpnmenu.h" +#include "userface.h" +#include "fort.h" +#include "misc.h" + +#define HELPENV "STADHELP" + +/* --- statically declared global variables --- */ +static helpindex_t helpbase[MAXOPTS]; /* table of help pointers for opts */ +static int helperr; /* are we allowed to give help? */ +static FILE *helpfp; /* FILE pointer for help */ +static int linesleft; /* no. of lines left in help */ +static int currentopt; /* which option we are now using */ + +/* --- globaly declared variables --- */ + +helpindex_t *helpindex = &helpbase[MAXMENUS]; +char *helptopics[MAXOPTS]; +int optTransTab[MAXOPTS]; +int maxopts; + +/* ---------------------- help functions --------------------------------- */ + +/* + * Supplies a line of help on the current option. + * Returns: + * The next line of help for this option if one exists. + * Otherwise NULL. + */ +char *gethelp() { + static char helpbuf[256]; + + if (helperr) + return NULL; + + if (linesleft) { + /* already got the help open */ + if (!--linesleft) { + /* need to press a key at the end of the help */ + (void)bpause(); + return NULL; + } + else + return fgets(helpbuf, sizeof(helpbuf), helpfp); + } else { + /* new help topic */ + if (currentopt < -MAXMENUS || currentopt > MAXOPTS) + linesleft = 0; + else + linesleft = helpindex[currentopt].lines; + if (linesleft == 0) { + linesleft = 1; + sprintf(helpbuf, " No help available on option %d\n", + currentopt); + return helpbuf; + } + fseek(helpfp, (size_t)helpindex[currentopt].offset, SEEK_SET); + sprintf(helpbuf, " Help on '%s' (option %d)\n", + helpindex[currentopt].name, currentopt); + return helpbuf; + } +} + +/* + * Supplies a line of help on a specific function. + * Args: + * optnum: which function to find help for. + * Returns: + * a line of help. (NULL if none) + */ +char *gethelp2(int optnum) { + int co = currentopt; + char *h; + + if (optnum >= MAXOPTS-MAXMENUS) + return NULL; + /* bit of a hack - but it works fine. */ + currentopt = optnum; + h = gethelp(); + currentopt = co; + return h; +} + +/* + * Initialises the help routines. + * Args: + * progname: the name of this program (or at least which programs help + * to use) + * numopts : maximum number of options used + * Returns: + * -1 for error + * 0 for success + */ +int inithelp(char *progname, int numopts) { + FILE *hp; + int optnum, menunum, offset, lines, hind = 0; + char prog, name[256], help_file[MAXPATHLEN], menu_file[MAXPATHLEN]; + char *stadenenv, mode; + + maxopts = numopts; + + /* what type of program are we? */ + mode = "TX"[xversn_()]; + + /* generate the pathnames of the files to use */ + if ((stadenenv = getenv(HELPENV)) == NULL) { + printf("Error - couldn't find environment variable '%s'\n", + HELPENV); + exit(1); + } + sprintf(help_file, "%s/%s_help", stadenenv, progname); + sprintf(menu_file, "%s/%s_menu", stadenenv, progname); + + helperr = 0; + currentopt = 0; + linesleft = 0; + + /* open up the help files */ + if ((hp = fopen(menu_file, "r")) == NULL) { + perror(menu_file); + exit(1); + } + if ((helpfp = fopen(help_file, "r")) == NULL) { + perror(help_file); + helperr = 1; + return -1; + } + + for (optnum = 0; optnum < MAXOPTS; optnum++) { + helpbase[optnum].menus = 0; + helpbase[optnum].name = 0; + for (menunum = 0; menunum < MAXDUP; menunum++) + helpbase[optnum].menunum[menunum] = -1; + } + + /* read in the menu index for use in the help/menu functions */ + while(fscanf(hp, "%d %d %d %d %c %[^\n]", &optnum, &menunum, &offset, + &lines, &prog, name) > 0) { + if (prog == mode) { + if (optnum > MAXOPTS || optnum < -MAXMENUS) { + puts("Too many options for menu table"); + fclose(hp); + return -1; + } + helpindex[optnum].menunum[helpindex[optnum].menus] = menunum; + if (helpindex[optnum].menus == 0) { + helpindex[optnum].offset = offset; + helpindex[optnum].lines = lines; + helpindex[optnum].name = strdup(name); + } + helpindex[optnum].menus++; + } + } + + /* help table (in option number order) - no help on menus! */ + helptopics[hind] = helpindex[0].name; + optTransTab[hind++] = 0; + + for (optnum = xversn_()?3:1; optnum < MAXOPTS-MAXMENUS; optnum++) + if (helpindex[optnum].name) { + helptopics[hind] = helpindex[optnum].name; + optTransTab[hind++] = optnum; + } + + /* make sure helptopics ends in a NULL (to be of XtNlist type */ + helptopics[hind] = NULL; + + fclose(hp); + return 0; +} + +void inthlp_x(char *PROG_p, int_f *NUMOPTS_p, int_fl PROG_l) { + char prog[256]; + + Fstr2Cstr(PROG_p, PROG_l, prog, (int_fl)sizeof(prog)); + inithelp(prog, (int)*NUMOPTS_p); +} + +/* ---------------------- menu functions --------------------------------- */ + +/* + * Create a list of menu items. + * Args: + * menunum: The menu number. + * menu : pointer to menu list (as an array). Each element of the array + * is a structure containing the name and option number. + * Returns + * How many items are in this menu. + */ +int create_menu(int menunum, menuarr *menu, int menusize) { + int i, ii, m; + + if (menunum < 0) + menunum = 0; +#if 0 + /* + * It's not really our job to do the deallocation - but for tidyness + * sake we try not to waste any memory + */ + for (m=0; m-MAXMENUS; i--) + if (helpindex[i].menunum[0] == menunum && helpindex[i].name) { + /* menu[m].name = strdup(helpindex[i].name); */ + menu[m].name = helpindex[i].name; + sprintf(menu[m].optname, "m%d", -i); + menu[m].number = i; + m++; + } + for (i=0; i +#include + +Semaphore activeLock; + +Semaphore semaphoreCreate(int max) +{ + Semaphore sem; + + if ((sem = (Semaphore) malloc (sizeof(SemaphoreStruct)))!=NULL) { + sem->count = 0; + sem->max = max; + } + return sem; +} + +int semaphoreGrab(Semaphore sem) +{ + return (sem->count==sem->max)?0:sem->count++,1; +} + +int semaphoreRelease(Semaphore sem) +{ + return (sem->count==0)?0:sem->count--,1; +} + +int semaphoreGrabN(Semaphore sem, int n) +{ + return (sem->count+n>sem->max)?0:(sem->count+=n),1; +} + +int semaphoreReleaseN(Semaphore sem, int n) +{ + return (sem->countcount-=n),1; +} + +int semaphoreGrabExclusive(Semaphore sem) +{ + return semaphoreGrabN(sem, sem->max); +} + +int semaphoreUsed(Semaphore sem) +{ + return sem->count; +} + +int semaphoreFree(Semaphore sem) +{ + return (sem->count == 0); +} + +Flag flagCreate() +{ + return (Flag) semaphoreCreate(1); +} + +int flagSet(Flag flag) +{ + return semaphoreGrab((Semaphore) flag); +} + +int flagUnset(Flag flag) +{ + return semaphoreRelease((Semaphore) flag); +} + +int flagUsed(Flag flag) +{ + return (flag->count); +} + +int flagFree(Flag flag) +{ + return (flag->count == 0); +} diff --git a/src/staden/locks.h b/src/staden/locks.h new file mode 100644 index 0000000..bf0cb11 --- /dev/null +++ b/src/staden/locks.h @@ -0,0 +1,52 @@ +#ifndef _locks_h +#define _locks_h +/* + Title: locks + + File: locks.h + Purpose: General routines for locking resources + Last update: +*/ + + +typedef struct { + int count; + int max; + } SemaphoreStruct, *Semaphore, *Flag; + + +extern Semaphore activeLock; + +#ifdef noddy + +#define semaphoreCreate(S,N) (S=(Semaphore)malloc(sizeof(Semaphore)))!=NULL)?S->count=0,S->max=N,S:S +#define semaphoreGrab(S) (S->count==S->max)?0:S->count++,1 +#define semaphoreRelease(S) (S->count==0)?0:S->count--,1 +#define semaphoreGrabN(S,N) (S->count+N>S->max)?0:(S-count+=N),1 +#define semaphoreRealeaseN(S,N) (S->countcount-=N),1 +#define semaphoreGrabExclusive(S) semaphoreGrabN(S,S->max) +#define semaphoreUsed(S) S->count +#define semaphoreFree(S) (S->count==0) +#define flagCreate (Flag) semaphoreCreate(1) +#define flagSet(F) semaphoreGrab((Semaphore) F) +#define flagUnset(F) semaphoreRelease((Semaphore) F) +#define flagUsed(F) F->count +#define flagFree(F) (F->count==0) + +#else +extern Semaphore semaphoreCreate(int max); +extern int semaphoreGrab(Semaphore sem); +extern int semaphoreRelease(Semaphore sem); +extern int semaphoreGrabN(Semaphore sem, int n); +extern int semaphoreReleaseN(Semaphore sem, int n); +extern int semaphoreGrabExclusive(Semaphore sem); +extern int semaphoreUsed(Semaphore sem); +extern int semaphoreFree(Semaphore sem); +extern Flag flagCreate(); +extern int flagSet(Flag flag); +extern int flagUnset(Flag flag); +extern int flagUsed(Flag flag); +extern int flagFree(Flag flag); +#endif + +#endif /* _locks_h */ diff --git a/src/staden/main.c b/src/staden/main.c new file mode 100644 index 0000000..bb88eba --- /dev/null +++ b/src/staden/main.c @@ -0,0 +1,400 @@ +/* + Title: main + + File: main.c + Purpose: C language entry point and initialisation functions + Last update: Wed Jan 23 1991 + + Change log: + 23/01/90 SD Resorce *yesno.YES.borderWidth now 3 + +*/ + + +/* + This module contains the C language entry point `main' and + initialisation for the X system. It provides the main event loop, + as far as X is concerned. + + Three top-level shells are created: control, dialogue and help. +*/ + + + + +/* ---- Includes ---- */ + +#include /* IMPORT: freopen, stdout, stderr, fprintf */ +#include +#include "mystdlib.h" /* IMPORT: exit */ + +#include "main.h" +#include "dialogues.h" /* IMPORT: CreateDialogueShell */ +#include "help.h" /* IMPORT: CreateHelpShell */ +#include "plX.h" /* IMPORT: CreateGraphicsOutput */ +#include "textOutput.h" /* IMPORT: CreateTextOutput + UpdateTextOutput */ +#include "postscript.h" /* IMPORT: closep_x() */ + +#include "locks.h" +#include "mcspec.h" +#include +#include +#include +#include + + + + +/* ---- Types ---- */ +typedef enum {Alive, Dying, Dead} LifeForce; + + + + +/* ---- Static variables ---- */ + +static int global_argc; +static char **global_argv; +static XtAppContext app_con; +static Display *display; +static Widget toplevelWid; +static Widget dialogueShellWid, helpShellWid, outputShellWid, graphicsShellWid; +static LifeForce lifeForce = Alive; +static int exitcode; +/* +static XrmOptionDescRec options[] = +{ +}; +*/ +/* The following are used if Xstaden cannot be found */ +static String fallback_resources[] = { + /* Dialogue shell */ + /* -------------- */ + "Xstaden.Dialgue.allowShellResize: False", + "Xstaden.Dialogue.width: 550", + "Xstaden.Dialogue.minWidth: 550", + "Xstaden.Dialogue.maxWidth: 550", + "Xstaden.Dialogue.height: 180", + "Xstaden.Dialogue.minHeight: 180", + "Xstaden.Dialogue.maxHeight: 180", + "Xstaden.Dialogue.dialogueForm*translations: #override \\n\ + Return: CRAction()", + /* The default chaining for all children is Top,Top,Left,Left */ + "Xstaden.Dialogue.dialogueForm*top: chainTop", + "Xstaden.Dialogue.dialogueForm*bottom: chainTop", + "Xstaden.Dialogue.dialogueForm*left: chainLeft", + "Xstaden.Dialogue.dialogueForm*right: chainLeft", + /* currFile */ + "Xstaden.Dialogue.dialogueForm.currFile.label: Current file:", + "Xstaden.Dialogue.dialogueForm.currFile.borderWidth: 0", + "Xstaden.Dialogue.dialogueForm.currFile.resizable: True", + /* currFunc */ + "Xstaden.Dialogue.dialogueForm.currFunc.label: Current function:", + "Xstaden.Dialogue.dialogueForm.currFunc.borderWidth: 0", + "Xstaden.Dialogue.dialogueForm.currFunc.resizable: True", + /* quit */ + "Xstaden.Dialogue.dialogueForm.quit.label: Quit application", + "Xstaden.Dialogue.dialogueForm.quit.foreground: red", + "Xstaden.Dialogue.dialogueForm.quit.borderColor: red", + + /* null */ + "Xstaden.Dialogue.dialogueForm.null.borderWidth: 0", + "Xstaden.Dialogue.dialogueForm.null.label: Working ... ", + + /* nChoice */ + "Xstaden.Dialogue.dialogueForm.nChoice.resizable: True", + /* nChoice buttons */ + "Xstaden.Dialogue.dialogueForm.nChoice.buttons.resizable: True", + /* nChoice OK */ + "Xstaden.Dialogue.dialogueForm.nChoice.OK.borderWidth: 3", + + /* getopt */ + "Xstaden.Dialogue.dialogueForm.getopt.width: 540", + /* getopt execlabel */ + "Xstaden.Dialogue.dialogueForm.getopt.execlabel.label: Select a function to:", + "Xstaden.Dialogue.dialogueForm.getopt.execlabel.borderWidth: 0", + /* getopt execcommand */ + "Xstaden.Dialogue.dialogueForm.getopt.execcommand.label: Execute with dialogue", + "Xstaden.Dialogue.dialogueForm.getopt.execcommand.resizable: True", + /* getopt menubar */ + "Xstaden.Dialogue.dialogueForm.getopt.menubar.width: 530", + "Xstaden.Dialogue.dialogueForm.getopt.menubar.height: 25", + + /* getstring */ + "Xstaden.Dialogue.dialogueForm.getstring.label:", + "Xstaden.Dialogue.dialogueForm.getstring.value:", + /* getstring label */ + "Xstaden.Dialogue.dialogueForm.getstring.label.resizable: True", + /* getstring value */ + "Xstaden.Dialogue.dialogueForm.getstring.value.width: 350", + /* getstring OK */ + "Xstaden.Dialogue.dialogueForm.getstring.OK.borderWidth: 3", + + /* yesno */ + "Xstaden.Dialogue.dialogueForm.yesno.resizable: True", + /* yesno label */ + "Xstaden.Dialogue.dialogueForm.yesno.lab.label:", + "Xstaden.Dialogue.dialogueForm.yesno.lab.borderWidth: 0", + "Xstaden.Dialogue.dialogueForm.yesno.lab.resizable: True", + "Xstaden.Dialogue.dialogueForm.yesno.YES.borderWidth: 3", + + /* Help shell */ + /* ---------- */ + "Xstaden.Help.width: 550", + "Xstaden.Help.minWidth: 550", + "Xstaden.Help.height: 400", + "Xstaden.Help.minHeight: 150", + "Xstaden.Help*top: chainTop", + "Xstaden.Help*bottom: chainTop", + "Xstaden.Help*left: chainLeft", + "Xstaden.Help*right: chainLeft", + /* currDisp */ + "Xstaden.Help.helpForm.currDisp.resizable: True", + "Xstaden.Help.helpForm.currDisp.borderWidth: 0", + /* rmHelp */ + "Xstaden.Help.helpForm.rmHelp.label: Remove help", + /* topicsViewport */ + "Xstaden.Help.helpForm.topicsViewport.allowVert: True", + "Xstaden.Help.helpForm.topicsViewport.bottom: chainBottom", + "Xstaden.Help.helpForm.topicsViewport.right: chainRight", + "Xstaden.Help.helpForm.topicsViewport.topicsList.defaultColumns: 1", + /* text */ + "Xstaden.Help.helpForm.text.editType: edit", + "Xstaden.Help.helpForm.text.scrollVertical: always", + "Xstaden.Help.helpForm.text.bottom: chainBottom", + "Xstaden.Help.helpForm.text.right: chainRight", + + /* Output shell */ + /* ------------ */ + "Xstaden.Graphics.graph.width: 550", + "Xstaden.Graphics.graph.height: 400", + "Xstaden.Output.text.width: 550", + "Xstaden.Output.text.height: 100", + + NULL, +}; + + + + +/* ---- Callback routines ---- */ + + +static void destroyCallback(Widget w, XtPointer client_data, XtPointer call_data) +{ lifeForce = Dead; +} + + + + +/* ---- Other functions ---- */ + + +static void usage(int argc, char **argv) +{ int i; + + fprintf(stderr, "%s: unknown options:", argv[0]); + for (i=1; i /* IMPORT: Boolean */ + + + + +/* ---- Exports ---- */ + + +extern void initx_(void); +/* + Initialise the X system, creating all necessary fixtures and + fittings for the application. + `stdout' may be redirected. +*/ + + +extern void finalx(int status); +/* + Recover all X resources and exit with `status'. +*/ + + +extern void dispatchEventsUntil(Boolean (*stopPred)(void)); +/* + X events are cycled until the stopping predicate returns true or + we are in the process of dying. If this is the case then this + function does not return. +*/ + + +extern void dispatchCurrentEvents(); +/* + All X events in the queue are cycled. If we are in the process of + dying, then the second phase of finalisation is carried out and + this function does not return. +*/ + + +extern Display *GetDisplay(void); +/* + Return the X display running. + This function can only be called after initx_(). +*/ diff --git a/src/staden/makefile-aix b/src/staden/makefile-aix new file mode 100644 index 0000000..d369cfa --- /dev/null +++ b/src/staden/makefile-aix @@ -0,0 +1,589 @@ +# Makefile for staden X programs (IBM AIX RS/6000) +# ------------------------------------------------ +# Many thanks to Klaus Hartmuth for making this version possible +# + +# This makefile generates the following staden programs which +# run under xterm (normal, `N') and those which run directly under X. +NPROGS= mep nip pip dap sap sip splitp1 splitp2 splitp3 gip sethelp convert_project sapf nipf vep +LPROGS= nipl pipl sipl +XPROGS= xmep xnip xpip xdap xsap xsip +PROGS= $(NPROGS) $(LPROGS) $(XPROGS) + + + + + + + +# +# ``Interesting'' suffices that make needs to know about +# +#.SUFFIXES: .a $(SUFFIXES) + + +# +# Where the wild things are +# +SRC=. +TEDSRC=$(SRC)/../ted +MISCSRC=$(SRC)/../Misc +MISCBIN=$(MISCSRC) +XINCLUDE= /usr/local/X11R5/include +INCLUDES= -I$(SRC) -I$(TEDSRC) -I$(MISCSRC) -I$(XINCLUDE) + + +# +# X11R5 & UTILITIES lib +# + +X11R5LIBS= -L/usr/local/X11R5/lib -lXaw -lXt -lXmu -lXext -lX11 +UTILITIES= -L$(HOME)/lib + +LIBS= $(UTILITIES) $(X11R5LIBS) + + +# +# Compilers and flags +# +CC= cc +CFLAGS= $(INCLUDES) -DAIX -qlanglvl=extended +CLFLAGS=$(LIBS) + +F77= xlf +FFLAGS= -qcharlen=1024 -NN500 -NP500 +FLFLAGS= + +LFLAGS= -lxlf -lc -lxlfutil $(ADDLIBS) +XLFLAGS= $(LIBS) + +# +# type of linking requested +# +LD=$(F77) +LDFLAGS=$(FLFLAGS) -bnodelcsect + + + +# +# Sequence library handling routines +# +SEQLIB=\ + seqlibsubs.o + + +# +# The C objects, needed by every X program +# +CCORE=\ + Graph.o\ + plotLog.o\ + help.o\ + mcspec.o\ + dialogues.o\ + locks.o + +COBJS=\ + main.o\ + $(CCORE) + +XDAPCOBJS=\ + xdapmain.o\ + $(CCORE) + + +# +# Common objects, needed by every program +# +COMMONOBJS=\ + fmain.o\ + seeme-aix.o\ + aix-bits.o\ + nxspec.o\ + userface.a\ + subs89.a\ + userface.a\ + subs89.a + +XCOMMONOBJS=\ + seeme-aix.o\ + aix-bits.o\ + xspec.o\ + subs89.a\ + $(COBJS) + +XDAPCOMMONOBJS=\ + seeme-aix.o\ + aix-bits.o\ + xspec.o\ + subs89.a\ + $(XDAPCOBJS) + + +# +# Building the programs +# This should be just a linking phase because all of the object +# files and library files are generated using implicit rules. +# We use the fortran compiler to do linking. +# +GIP=\ + gip.o + +GIPOBJS=\ + $(GIP)\ + $(COMMONOBJS) + +gip: $(GIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(GIPOBJS) $(LFLAGS) + + + + +MEP=\ + mep.o\ + mepsub.o\ + asubs89.a\ + plot89.a + +MEPOBJS=\ + $(MEP)\ + mepmen.o\ + $(COMMONOBJS)\ + pl4010.o + +XMEPOBJS=\ + $(MEP)\ + xmepSpec.o\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +mep: $(MEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(MEPOBJS) $(LFLAGS) + +xmep: $(XMEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XMEPOBJS) $(XLFLAGS) + + + + +NIP=\ + nip.o\ + patternn.a\ + patternnc.a\ + anals89.a\ + asubs89.a\ + plot89.a $(SEQLIB) + +NIPOBJS=\ + $(NIP)\ + nipmen.o\ + $(COMMONOBJS)\ + pl4010.o + +XNIPOBJS=\ + $(NIP)\ + xnipSpec.o\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +nip: $(NIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPOBJS) $(LFLAGS) + +xnip: $(XNIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XNIPOBJS) $(XLFLAGS) + + + + +NIPL=\ + nipl.o\ + patternnc.a\ + anals89.a\ + asubs89.a\ + $(SEQLIB) + +NIPLOBJS=\ + $(NIPL)\ + $(COMMONOBJS) + +nipl: $(NIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPLOBJS) $(LFLAGS) + + + +NIPF=\ + nipf.o\ + asubs89.a\ + plot89.a + +NIPFOBJS=\ + $(NIPF)\ + $(COMMONOBJS)\ + pl4010.o + + +nipf: $(NIPFOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPFOBJS) $(LFLAGS) + + + + + +PIP=\ + pip.o\ + analps89.a\ + patternp.a\ + patternpc.a\ + asubs89.a\ + plot89.a\ + $(SEQLIB) + +PIPOBJS=\ + $(PIP)\ + pipmen.o\ + $(COMMONOBJS)\ + pl4010.o + +XPIPOBJS=\ + $(PIP)\ + xpipSpec.o\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +pip: $(PIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(PIPOBJS) $(LFLAGS) + +xpip:$(XPIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XPIPOBJS) $(XLFLAGS) + + + + +PIPL=\ + pipl.o\ + patternpc.a\ + analps89.a\ + asubs89.a\ + $(SEQLIB) + +PIPLOBJS=\ + $(PIPL)\ + $(COMMONOBJS) + +pipl: $(PIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(PIPLOBJS) $(LFLAGS) + + + +# +# Trace manager objects +# +STDTEDFILES=\ + seq.o\ + seqIOABI.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqIOPlain.o\ + opp.o\ + fpoint.o\ + mach-io.o + +TMANOBJS=\ + tman_main.o\ + tman_display.o\ + tman_traceDisplay.o\ + tman_basesDisplay.o\ + tman_context.o\ + tman_gadgets.o\ + $(STDTEDFILES) + +# Some versions of X11R4 may have a bug in SmeLine.c +XHACK=\ + SmeLine.o + +EDITOR=\ + $(XHACK)\ + xsapConEdit.o\ + contigEditor.o\ + edUtils.o\ + undo.o\ + Sheet.o\ + select.o\ + extend.o\ + searchUtils.o\ + edMenu.o\ + trans.o\ + $(MISCBIN)/misc.a + +TAGEDITOR=\ + tagEditor.o\ + tagdbparse.o\ + tagU2.o\ + tagU1.o + +DAP=\ + dap.o\ + dbsysnew.o\ + dbsyscommon.o\ + asubs89.a\ + plot89.a + +DAPOBJS=\ + $(DAP)\ + sapmen.o\ + $(COMMONOBJS)\ + pl4010.o\ + conEdit.o\ + tagU2.o + +XDAPOBJS=\ + $(DAP)\ + xdapSpec.o\ + $(XDAPCOMMONOBJS)\ + plX.o\ + textOutput.o\ + $(EDITOR)\ + $(TMANOBJS)\ + $(TAGEDITOR) + +dap: $(DAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(DAPOBJS) $(LFLAGS) + +xdap: $(XDAPOBJS) + $(CC) $(CFLAGS) -o $@ $(XDAPOBJS) $(XLFLAGS) + +convert_project: cvt.o + $(CC) $(CFLAGS) -o $@ cvt.o + +SAP=\ + sap.o\ + dbsyscommon.o\ + dbsysold.o\ + asubs89.a\ + plot89.a + +SAPOBJS=\ + $(SAP)\ + sapmen.o\ + $(COMMONOBJS)\ + pl4010.o + +XSAPOBJS=\ + $(SAP)\ + xsapSpec.o\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +sap: $(SAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SAPOBJS) $(LFLAGS) + +xsap: $(XSAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XSAPOBJS) $(XLFLAGS) + + + + +SAPF=\ + sapf.o\ + dbsyscommon.o\ + dbsysold.o\ + asubs89.a\ + plot89.a + +SAPFOBJS=\ + $(SAPF)\ + sapmen.o\ + $(COMMONOBJS)\ + pl4010.o + +sapf: $(SAPFOBJS) + $(LD) $(LDFLAGS) -o $@ $(SAPFOBJS) $(LFLAGS) + + + + + + +SIP=\ + sip.o\ + dias89.a\ + plot89.a\ + $(SEQLIB) + +SIPOBJS=\ + $(SIP)\ + sipmen.o\ + $(COMMONOBJS)\ + pl4010.o + +XSIPOBJS=\ + $(SIP)\ + xsipSpec.o\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +sip: $(SIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SIPOBJS) $(LFLAGS) + +xsip: $(XSIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XSIPOBJS) $(XLFLAGS) + + + + +SIPL=\ + sipl.o\ + dias89.a\ + $(SEQLIB) + +SIPLOBJS=\ + $(SIPL)\ + $(COMMONOBJS) + +sipl: $(SIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(SIPLOBJS) $(LFLAGS) + + + + +SETHELP=\ + sethelp.o + +SETHELPOBJS=\ + $(SETHELP)\ + $(COMMONOBJS) + +sethelp: $(SETHELPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SETHELPOBJS) $(LFLAGS) + + + + +SPLITP1=\ + splitp1.o +SPLITP1OBJS=\ + $(SPLITP1)\ + $(COMMONOBJS) + +splitp1: $(SPLITP1OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP1OBJS) $(LFLAGS) + + + + +SPLITP2=\ + splitp2.o +SPLITP2OBJS=\ + $(SPLITP2)\ + $(COMMONOBJS) + +splitp2: $(SPLITP2OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP2OBJS) $(LFLAGS) + + + + + +SPLITP3=\ + splitp3.o +SPLITP3OBJS=\ + $(SPLITP3)\ + $(COMMONOBJS) + +splitp3: $(SPLITP3OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP3OBJS) $(LFLAGS) + + + + + + + + + +VEP=\ + vep.o\ + dias89.a\ + subs89.a + + +VEPOBJS=\ + $(VEP)\ + $(COMMONOBJS) + + +vep: $(VEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPOBJS) $(LFLAGS) + + +# +# Special dependencies +# +# + +# Files requiring simple FORTRAN compilation (thing.f -> thing.o) +# +.f.o: + $(F77) $(FFLAGS) -c $< + + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +.c.o: + $(CC) $(CFLAGS) -c $< + + + +# +# The UNIX library (archive) mechanism is fairly weak so the following +# procedure is used to generate libraries. +# * split the source file up into files which each contain +# a single function or subroutine +# * compile each of those files +# * insert the object files individually into the library +# Each library is built by using a temporary directory. +# The fortran compilations must be done sequentially in order to +# avoid filling up the (small on this machine) /tmp directory +# with compiler debugging information. +# +#.f.a: +# BINDIR=`pwd`; \ +# cd $(SRC); \ +# SRCDIR=`pwd`; \ +# TMPDIR=/tmp/staden$$$$; \ +# if test ! -d $$TMPDIR; \ +# then mkdir $$TMPDIR; \ +# fi; \ +# cd $$TMPDIR; \ +# rm -f *.f *.o; \ +# fsplit $$SRCDIR/`expr $< : '.*/\(.*\)' \| $<`; \ +# $(F77) $(FFLAGS) -c *.f; \ +# rm *.f; \ +# rm -f $$BINDIR/$@; \ +# ar rcv $$BINDIR/$@ *.o; \ +# ranlib $$BINDIR/$@; \ +# rm *.o; \ +# cd $$BINDIR; \ +# rm -r $$TMPDIR +# + + + + +all: $(PROGS) + +nprogs: $(NPROGS) + +xprogs: $(XPROGS) + +lprogs: $(LPROGS) + +clean: + rm -f $(PROGS) *.a *.o *~ + diff --git a/src/staden/makefile-alpha b/src/staden/makefile-alpha new file mode 100644 index 0000000..f9b9043 --- /dev/null +++ b/src/staden/makefile-alpha @@ -0,0 +1,592 @@ +# Makefile for staden X programs (Alpha OSF/1.2 version) +# ------------------------------------------------------ + +# This makefile generates the following staden programs which +# run under xterm (normal, `N') and those which run directly under X. +NPROGS= mep nip pip dap sap sip splitp1 splitp2 splitp3 gip sethelp convert_project rep lip sapf nipf vep +LPROGS= nipl pipl sipl +XPROGS= xmep xnip xpip xdap xsap xsip +PROGS= $(NPROGS) $(LPROGS) $(XPROGS) + +# +# ``Interesting'' suffices that make needs to know about +# +.SUFFIXES: .a $(SUFFIXES) + +# +# Where the wild things are +# +SRC=. +TEDSRC=$(SRC)/../ted +MISCSRC=$(SRC)/../Misc +MISCBIN=$(MISCSRC)/alpha-binaries +INCLUDES= -I$(SRC) -I$(TEDSRC) -I$(MISCSRC) + +# +# Compilers and flags +# +CC= cc +CFLAGS= -g -std1 -ieee_with_inexact $(INCLUDES) +F77= f77 +FFLAGS= -g -C + +FLIBS= -lfor -lutil -lUfor -lots -lFutil -lm -lfor -lutil -lUfor -lots -lFutil +XLIBS= -lXaw -lXt -lXmu -lXext -lX11 -ldnet_stub +LFLAGS= $(FLIBS) +XLFLAGS= $(FLIBS) $(XLIBS) + +LD = f77 +CLD = cc +LDFLAGS = -g -non_shared # Comment out for faster linking when debugging + + + +# +# Sequence library handling routines +# +SEQLIB=\ + seqlibsubs.o + + +# +# The C objects, needed by every X program +# +CCORE=\ + postscript.o\ + Graph.o\ + plotLog.o\ + help.o\ + dialogues.o\ + userfacecom.o\ + helpnmenu.o\ + xmenu.o\ + FtoC.o\ + mcspec.o\ + locks.o + +COBJS=\ + main.o\ + $(CCORE) + +XDAPCOBJS=\ + xdapmain.o\ + $(CCORE) + + +# +# Common objects, needed by every program +# +COMMONOBJSB=\ + fmain.o\ + seeme-dec.o\ + nxspec.o\ + userface.o\ + userfacecom.o\ + nxhelpmenu.o\ + helpnmenu.o\ + FtoC.o\ + subs89.a + +COMMONOBJS=\ + $(COMMONOBJSB)\ + postscript.o + +SCOMMONOBJS=\ + $(COMMONOBJSB) + +XCOMMONOBJS=\ + seeme-dec.o\ + xspec.o\ + subs89.a\ + $(COBJS) + +XDAPCOMMONOBJS=\ + seeme-dec.o\ + xspec.o\ + subs89.a\ + $(XDAPCOBJS) + + +# +# Building the programs +# This should be just a linking phase because all of the object +# files and library files are generated using implicit rules. +# We use the fortran compiler to do linking. +# +GIP=\ + gip.o + +GIPOBJS=\ + $(GIP)\ + $(SCOMMONOBJS) + +gip: $(GIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(GIPOBJS) $(LFLAGS) + + +LIP=\ + lip.o + +LIPOBJS=\ + $(LIP)\ + $(SEQLIB)\ + $(COMMONOBJS) + +lip: $(LIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(LIPOBJS) $(LFLAGS) + + +MEP=\ + mep.o\ + mepsub.o\ + asubs89.a\ + plot92.a + +MEPOBJS=\ + $(MEP)\ + $(COMMONOBJS)\ + pl4010.o + +XMEPOBJS=\ + $(MEP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +mep: $(MEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(MEPOBJS) $(LFLAGS) + +xmep: $(XMEPOBJS) + $(CLD) $(LDFLAGS) -o $@ $(XMEPOBJS) $(XLFLAGS) + + + + +NIP=\ + nip.o\ + patternn.a\ + patternnc.a\ + anals89.a\ + asubs89.a\ + plot92.a \ + $(SEQLIB) + +NIPOBJS=\ + $(NIP)\ + $(COMMONOBJS)\ + pl4010.o + +XNIPOBJS=\ + $(NIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +nip: $(NIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPOBJS) $(LFLAGS) + +xnip: $(XNIPOBJS) + $(CLD) $(LDFLAGS) -o $@ $(XNIPOBJS) $(XLFLAGS) + + + + +NIPL=\ + nipl.o\ + patternnc.a\ + anals89.a\ + asubs89.a\ + $(SEQLIB) + +NIPLOBJS=\ + $(NIPL)\ + $(SCOMMONOBJS) + +nipl: $(NIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPLOBJS) $(LFLAGS) + + + +NIPF=\ + nipf.o\ + asubs89.a\ + plot92.a + +NIPFOBJS=\ + $(NIPF)\ + $(COMMONOBJS)\ + pl4010.o + + +nipf: $(NIPFOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPFOBJS) $(LFLAGS) + + + + + +PIP=\ + pip.o\ + analps89.a\ + patternp.a\ + patternpc.a\ + asubs89.a\ + plot92.a\ + $(SEQLIB) + +PIPOBJS=\ + $(PIP)\ + $(COMMONOBJS)\ + pl4010.o + +XPIPOBJS=\ + $(PIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +pip: $(PIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(PIPOBJS) $(LFLAGS) + +xpip:$(XPIPOBJS) + $(CLD) $(LDFLAGS) -o $@ $(XPIPOBJS) $(XLFLAGS) + + + + +PIPL=\ + pipl.o\ + patternpc.a\ + analps89.a\ + asubs89.a\ + $(SEQLIB) + +PIPLOBJS=\ + $(PIPL)\ + $(SCOMMONOBJS) + +pipl: $(PIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(PIPLOBJS) $(LFLAGS) + + + +# +# Trace manager objects +# +STDTEDFILES=\ + seq.o\ + seqIOABI.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqIOPlain.o\ + opp.o\ + fpoint.o\ + mach-io.o + +TMANOBJS=\ + tman_main.o\ + tman_display.o\ + tman_traceDisplay.o\ + tman_basesDisplay.o\ + tman_context.o\ + tman_gadgets.o\ + $(STDTEDFILES) + +# Some versions of X11R4 may have a bug in SmeLine.c +XHACK=\ + SmeLine.o + +EDITOR=\ + $(XHACK)\ + xsapConEdit.o\ + contigEditor.o\ + edUtils.o\ + undo.o\ + Sheet.o\ + select.o\ + extend.o\ + searchUtils.o\ + edMenu.o\ + trans.o\ + $(MISCBIN)/misc.a + +TAGEDITOR=\ + tagEditor.o\ + tagdbparse.o\ + tagU2.o\ + tagU1.o + +DAP=\ + dap.o\ + dbsysnew.o\ + dbsyscommon.o\ + asubs89.a\ + plot92.a + +DAPOBJS=\ + $(DAP)\ + $(COMMONOBJS)\ + pl4010.o\ + conEdit.o\ + tagU2.o + +XDAPOBJS=\ + $(DAP)\ + $(XDAPCOMMONOBJS)\ + plX.o\ + textOutput.o\ + $(EDITOR)\ + $(TMANOBJS)\ + $(TAGEDITOR) + +dap: $(DAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(DAPOBJS) $(LFLAGS) + +xdap: $(XDAPOBJS) + $(CLD) $(LDFLAGS) -o $@ $(XDAPOBJS) $(XLFLAGS) + +convert_project: cvt.o + $(CC) $(CFLAGS) -o $@ cvt.o + +SAP=\ + sap.o\ + dbsyscommon.o\ + dbsysold.o\ + asubs89.a\ + plot92.a + +SAPOBJS=\ + $(SAP)\ + $(COMMONOBJS)\ + pl4010.o + +XSAPOBJS=\ + $(SAP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +sap: $(SAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SAPOBJS) $(LFLAGS) + +xsap: $(XSAPOBJS) + $(CLD) $(LDFLAGS) -o $@ $(XSAPOBJS) $(XLFLAGS) + + + + +SAPF=\ + sapf.o\ + dbsyscommon.o\ + dbsysold.o\ + asubs89.a\ + plot92.a + +SAPFOBJS=\ + $(SAPF)\ + sapmen.o\ + $(COMMONOBJS)\ + pl4010.o + +sapf: $(SAPFOBJS) + $(LD) $(LDFLAGS) -o $@ $(SAPFOBJS) $(LFLAGS) + + + + + + +SIP=\ + sip.o\ + dias89.a\ + plot92.a\ + $(SEQLIB) + +SIPOBJS=\ + $(SIP)\ + $(COMMONOBJS)\ + pl4010.o + +XSIPOBJS=\ + $(SIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +sip: $(SIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SIPOBJS) $(LFLAGS) + +xsip: $(XSIPOBJS) + $(CLD) $(LDFLAGS) -o $@ $(XSIPOBJS) $(XLFLAGS) + + + + +SIPL=\ + sipl.o\ + dias89.a\ + $(SEQLIB) + +SIPLOBJS=\ + $(SIPL)\ + $(SCOMMONOBJS) + +sipl: $(SIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(SIPLOBJS) $(LFLAGS) + + + + +SETHELP=\ + sethelp.o + +SETHELPOBJS=\ + $(SETHELP) + +sethelp: $(SETHELPOBJS) + $(CLD) $(LDFLAGS) -o $@ $(SETHELPOBJS) $(LFLAGS) + + + + +SPLITP1=\ + splitp1.o +SPLITP1OBJS=\ + $(SPLITP1)\ + $(SCOMMONOBJS) + +splitp1: $(SPLITP1OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP1OBJS) $(LFLAGS) + + + + +SPLITP2=\ + splitp2.o +SPLITP2OBJS=\ + $(SPLITP2)\ + $(SCOMMONOBJS) + +splitp2: $(SPLITP2OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP2OBJS) $(LFLAGS) + + + + + +SPLITP3=\ + splitp3.o +SPLITP3OBJS=\ + $(SPLITP3)\ + $(SCOMMONOBJS) + +splitp3: $(SPLITP3OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP3OBJS) $(LFLAGS) + + + +REP=\ + rep.o\ + dias89.a\ + subs89.a + +REPOBJS=\ + $(REP)\ + $(COMMONOBJS) + +rep: $(REPOBJS) + $(F77) $(LDFLAGS) -o $@ $(REPOBJS) $(LFLAGS) + + + + + +VEP=\ + vep.o\ + dias89.a\ + subs89.a + + +VEPOBJS=\ + $(VEP)\ + $(SCOMMONOBJS) + + +vep: $(VEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPOBJS) $(LFLAGS) + + + + +# +# Special dependencies +# +# + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< +%.o: $(TEDSRC)/%.c + $(CC) $(CFLAGS) -c $< + +# Files requiring simple FORTRAN compilation (thing.f -> thing.o) +# +%.o: $(SRC)/%.f + $(F77) $(FFLAGS) -c $< + + +# +# The UNIX library (archive) mechanism is fairly weak so the following +# procedure is used to generate libraries. +# * split the source file up into files which each contain +# a single function or subroutine +# * compile each of those files +# * insert the object files individually into the library +# Each library is built by using a temporary directory. +# The fortran compilations must be done sequentially in order to +# avoid filling up the (small on this machine) /tmp directory +# with compiler debugging information. +# +%.a: $(SRC)/%.f + BINDIR=`pwd`; \ + cd $(SRC); \ + SRCDIR=`pwd`; \ + TMPDIR=/tmp/staden$$$$; \ + if test ! -d $$TMPDIR; \ + then mkdir $$TMPDIR; \ + fi; \ + cd $$TMPDIR; \ + rm -f *.f *.o; \ + fsplit $$SRCDIR/`expr $< : '.*/\(.*\)' \| $<`; \ + $(F77) $(FFLAGS) -c *.f; \ + rm *.f; \ + rm -f $$BINDIR/$@; \ + ar rcv $$BINDIR/$@ *.o; \ + ranlib $$BINDIR/$@; \ + rm *.o; \ + cd $$BINDIR; \ + rm -r $$TMPDIR + +#.f.a: +# $(F77) $(FFLAGS) -c $< +# ar rcv $@ $*.o +# ranlib $@ +# rm $*.o + +all: $(PROGS) + +nprogs: $(NPROGS) + +xprogs: $(XPROGS) + +lprogs: $(LPROGS) + +clean: + rm -f $(PROGS) *.a *.o *~ + +lint: + lint -Q -Nn5000 -DNOSTRDUP $(INCLUDES) *.c + + diff --git a/src/staden/makefile-dec b/src/staden/makefile-dec new file mode 100644 index 0000000..2cfd305 --- /dev/null +++ b/src/staden/makefile-dec @@ -0,0 +1,595 @@ +# Makefile for staden X programs (Dec version) +# -------------------------------------------- + +# This makefile generates the following staden programs which +# run under xterm (normal, `N') and those which run directly under X. +NPROGS= mep nip pip dap sap sip splitp1 splitp2 splitp3 gip sethelp convert_project rep lip sapf nipf vep +LPROGS= nipl pipl sipl +XPROGS= xmep xnip xpip xdap xsap xsip +PROGS= $(NPROGS) $(LPROGS) $(XPROGS) + + + + + + + +# +# ``Interesting'' suffices that make needs to know about +# +.SUFFIXES: .a $(SUFFIXES) + + +# +# Where the wild things are +# +SRC=. +TEDSRC=$(SRC)/../ted +MISCSRC=$(SRC)/../Misc +MISCBIN=$(MISCSRC) +INCLUDES= -I/usr/include/mit -I$(SRC) -I$(TEDSRC) -I$(MISCSRC) + + +# +# Compilers and flags +# +CC= c89 +CFLAGS= -g -common -DNOSTRDUP $(INCLUDES) +F77= f77 +FFLAGS= -g -c + +#FLIBS= -lfor -lutil -lUfor -li -lots -lm +FLIBS= $(MISCBIN)/misc.a -lfor -lutil -lUfor -li -lots -lm -lfor +XLIBS= -lXaw -lXt -lXmu -lXext -lX11 +LFLAGS= $(FLIBS) +XLFLAGS= $(FLIBS) $(XLIBS) +#LFLAGS= +#XLFLAGS= $(XLIBS) +LD = c89 +LDFLAGS = -g + + + +# +# Sequence library handling routines +# +SEQLIB=\ + seqlibsubs.o + + +# +# The C objects, needed by every X program +# +CCORE=\ + postscript.o\ + Graph.o\ + plotLog.o\ + help.o\ + dialogues.o\ + userfacecom.o\ + helpnmenu.o\ + xmenu.o\ + FtoC.o\ + mcspec.o\ + locks.o + +COBJS=\ + main.o\ + $(CCORE) + +XDAPCOBJS=\ + xdapmain.o\ + $(CCORE) + + +# +# Common objects, needed by every program +# +COMMONOBJSB=\ + fmain.o\ + seeme-dec.o\ + nxspec.o\ + userface.o\ + userfacecom.o\ + nxhelpmenu.o\ + helpnmenu.o\ + FtoC.o\ + subs89.a + +COMMONOBJS=\ + $(COMMONOBJSB)\ + postscript.o + +SCOMMONOBJS=\ + $(COMMONOBJSB) + +XCOMMONOBJS=\ + seeme-dec.o\ + xspec.o\ + subs89.a\ + $(COBJS) + +XDAPCOMMONOBJS=\ + seeme-dec.o\ + xspec.o\ + subs89.a\ + $(XDAPCOBJS) + + +# +# Building the programs +# This should be just a linking phase because all of the object +# files and library files are generated using implicit rules. +# We use the fortran compiler to do linking. +# +GIP=\ + gip.o + +GIPOBJS=\ + $(GIP)\ + $(SCOMMONOBJS) + +gip: $(GIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(GIPOBJS) $(LFLAGS) + + +LIP=\ + lip.o + +LIPOBJS=\ + $(LIP)\ + $(SEQLIB)\ + $(COMMONOBJS) + +lip: $(LIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(LIPOBJS) $(LFLAGS) + + +MEP=\ + mep.o\ + mepsub.o\ + asubs89.a\ + plot92.a + +MEPOBJS=\ + $(MEP)\ + $(COMMONOBJS)\ + pl4010.o + +XMEPOBJS=\ + $(MEP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +mep: $(MEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(MEPOBJS) $(LFLAGS) + +xmep: $(XMEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XMEPOBJS) $(XLFLAGS) + + + + +NIP=\ + nip.o\ + patternn.a\ + patternnc.a\ + anals89.a\ + asubs89.a\ + plot92.a $(SEQLIB) + +NIPOBJS=\ + $(NIP)\ + $(COMMONOBJS)\ + pl4010.o + +XNIPOBJS=\ + $(NIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +nip: $(NIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPOBJS) $(LFLAGS) + +xnip: $(XNIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XNIPOBJS) $(XLFLAGS) + + + + +NIPL=\ + nipl.o\ + patternnc.a\ + anals89.a\ + asubs89.a\ + $(SEQLIB) + +NIPLOBJS=\ + $(NIPL)\ + $(SCOMMONOBJS) + +nipl: $(NIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPLOBJS) $(LFLAGS) + + + +NIPF=\ + nipf.o\ + asubs89.a\ + plot92.a + +NIPFOBJS=\ + $(NIPF)\ + $(COMMONOBJS)\ + pl4010.o + + +nipf: $(NIPFOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPFOBJS) $(LFLAGS) + + + + + +PIP=\ + pip.o\ + analps89.a\ + patternp.a\ + patternpc.a\ + asubs89.a\ + plot92.a\ + $(SEQLIB) + +PIPOBJS=\ + $(PIP)\ + $(COMMONOBJS)\ + pl4010.o + +XPIPOBJS=\ + $(PIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +pip: $(PIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(PIPOBJS) $(LFLAGS) + +xpip:$(XPIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XPIPOBJS) $(XLFLAGS) + + + + +PIPL=\ + pipl.o\ + patternpc.a\ + analps89.a\ + asubs89.a\ + $(SEQLIB) + +PIPLOBJS=\ + $(PIPL)\ + $(SCOMMONOBJS) + +pipl: $(PIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(PIPLOBJS) $(LFLAGS) + + + +# +# Trace manager objects +# +STDTEDFILES=\ + seq.o\ + seqIOABI.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqIOPlain.o\ + opp.o\ + fpoint.o\ + mach-io.o + +TMANOBJS=\ + tman_main.o\ + tman_display.o\ + tman_traceDisplay.o\ + tman_basesDisplay.o\ + tman_context.o\ + tman_gadgets.o\ + $(STDTEDFILES) + +# Some versions of X11R4 may have a bug in SmeLine.c +XHACK=\ + SmeLine.o + +EDITOR=\ + $(XHACK)\ + xsapConEdit.o\ + contigEditor.o\ + edUtils.o\ + undo.o\ + Sheet.o\ + select.o\ + extend.o\ + searchUtils.o\ + edMenu.o\ + trans.o\ + $(MISCBIN)/misc.a + +TAGEDITOR=\ + tagEditor.o\ + tagdbparse.o\ + tagU2.o\ + tagU1.o + +DAP=\ + dap.o\ + dbsysnew.o\ + dbsyscommon.o\ + asubs89.a\ + plot92.a + +DAPOBJS=\ + $(DAP)\ + $(COMMONOBJS)\ + pl4010.o\ + conEdit.o\ + tagU2.o + +XDAPOBJS=\ + $(DAP)\ + $(XDAPCOMMONOBJS)\ + plX.o\ + textOutput.o\ + $(EDITOR)\ + $(TMANOBJS)\ + $(TAGEDITOR) + +dap: $(DAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(DAPOBJS) $(LFLAGS) + +xdap: $(XDAPOBJS) + $(CC) $(CFLAGS) -o $@ $(XDAPOBJS) $(XLFLAGS) + +convert_project: cvt.o + $(CC) $(CFLAGS) -o $@ cvt.o + +SAP=\ + sap.o\ + dbsyscommon.o\ + dbsysold.o\ + asubs89.a\ + plot92.a + +SAPOBJS=\ + $(SAP)\ + $(COMMONOBJS)\ + pl4010.o + +XSAPOBJS=\ + $(SAP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +sap: $(SAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SAPOBJS) $(LFLAGS) + +xsap: $(XSAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XSAPOBJS) $(XLFLAGS) + + + + +SAPF=\ + sapf.o\ + dbsyscommon.o\ + dbsysold.o\ + asubs89.a\ + plot92.a + +SAPFOBJS=\ + $(SAPF)\ + sapmen.o\ + $(COMMONOBJS)\ + pl4010.o + +sapf: $(SAPFOBJS) + $(LD) $(LDFLAGS) -o $@ $(SAPFOBJS) $(LFLAGS) + + + + + + +SIP=\ + sip.o\ + dias89.a\ + plot92.a\ + $(SEQLIB) + +SIPOBJS=\ + $(SIP)\ + $(COMMONOBJS)\ + pl4010.o + +XSIPOBJS=\ + $(SIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +sip: $(SIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SIPOBJS) $(LFLAGS) + +xsip: $(XSIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XSIPOBJS) $(XLFLAGS) + + + + +SIPL=\ + sipl.o\ + dias89.a\ + $(SEQLIB) + +SIPLOBJS=\ + $(SIPL)\ + $(SCOMMONOBJS) + +sipl: $(SIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(SIPLOBJS) $(LFLAGS) + + + + +SETHELP=\ + sethelp.o + +SETHELPOBJS=\ + $(SETHELP) + +sethelp: $(SETHELPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SETHELPOBJS) $(LFLAGS) + + + + +SPLITP1=\ + splitp1.o +SPLITP1OBJS=\ + $(SPLITP1)\ + $(SCOMMONOBJS) + +splitp1: $(SPLITP1OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP1OBJS) $(LFLAGS) + + + + +SPLITP2=\ + splitp2.o +SPLITP2OBJS=\ + $(SPLITP2)\ + $(SCOMMONOBJS) + +splitp2: $(SPLITP2OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP2OBJS) $(LFLAGS) + + + + + +SPLITP3=\ + splitp3.o +SPLITP3OBJS=\ + $(SPLITP3)\ + $(SCOMMONOBJS) + +splitp3: $(SPLITP3OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP3OBJS) $(LFLAGS) + + +REP=\ + rep.o\ + dias89.a\ + subs89.a + +REPOBJS=\ + $(REP)\ + $(COMMONOBJS) + +rep: $(REPOBJS) + $(F77) $(LDFLAGS) -o $@ $(REPOBJS) $(LFLAGS) + + + +VEP=\ + vep.o\ + dias89.a\ + subs89.a + + +VEPOBJS=\ + $(VEP)\ + $(SCOMMONOBJS) + + +vep: $(VEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPOBJS) $(LFLAGS) + + + +# +# Special dependencies +# +# + + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o: %.c + $(CC) $(CFLAGS) -c $< +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< +%.o: $(TEDSRC)/%.c + $(CC) $(CFLAGS) -c $< + +# Files requiring simple FORTRAN compilation (thing.f -> thing.o) +# +%.o: $(SRC)/%.f + $(F77) $(FFLAGS) -c $< + + + +# +# The UNIX library (archive) mechanism is fairly weak so the following +# procedure is used to generate libraries. +# * split the source file up into files which each contain +# a single function or subroutine +# * compile each of those files +# * insert the object files individually into the library +# Each library is built by using a temporary directory. +# The fortran compilations must be done sequentially in order to +# avoid filling up the (small on this machine) /tmp directory +# with compiler debugging information. +# +%.a: $(SRC)/%.f + BINDIR=`pwd`; \ + cd $(SRC); \ + SRCDIR=`pwd`; \ + TMPDIR=/tmp/staden$$$$; \ + if test ! -d $$TMPDIR; \ + then mkdir $$TMPDIR; \ + fi; \ + cd $$TMPDIR; \ + rm -f *.f *.o; \ + fsplit $$SRCDIR/`expr $< : '.*/\(.*\)' \| $<`; \ + $(F77) $(FFLAGS) -c *.f; \ + rm *.f; \ + rm -f $$BINDIR/$@; \ + ar rcv $$BINDIR/$@ *.o; \ + ranlib $$BINDIR/$@; \ + rm *.o; \ + cd $$BINDIR; \ + rm -r $$TMPDIR + + + + + + +all: $(PROGS) + +nprogs: $(NPROGS) + +xprogs: $(XPROGS) + +lprogs: $(LPROGS) + +clean: + rm -f $(PROGS) *.a *.o *~ + diff --git a/src/staden/makefile-sgi b/src/staden/makefile-sgi new file mode 100644 index 0000000..b7bb6b4 --- /dev/null +++ b/src/staden/makefile-sgi @@ -0,0 +1,604 @@ +# Makefile for staden X programs (Silicon Graphics version) +# --------------------------------------------------------- + +# This makefile generates the following staden programs which +# run under xterm (normal, `N') and those which run directly under X. +NPROGS= mep nip pip dap sap sip splitp1 splitp2 splitp3 gip sethelp convert_project sapf nipf vep rep lip +LPROGS= nipl pipl sipl +XPROGS= xmep xnip xpip xdap xsap xsip +PROGS= $(NPROGS) $(LPROGS) $(XPROGS) + + + + + + + +# +# ``Interesting'' suffices that make needs to know about +# +.SUFFIXES: .a $(SUFFIXES) + + +# +# Where the wild things are +# +SRC=. +TEDSRC=$(SRC)/../ted +MISCSRC=$(SRC)/../Misc +MISCBIN=$(MISCSRC) +INCLUDES= -I$(SRC) -I$(TEDSRC) -I$(MISCSRC) + + +# +# Compilers and flags +# +CC= cc +CFLAGS= -g -ansi -prototypes $(INCLUDES) +F77= f77 +FFLAGS= -g -c -old_rl + +FLIBS= -lF77 -lU77 -lm -lI77 -lisam +XLIBS= -lXaw -lXt -lXmu -lXext -lX11 +LFLAGS= $(FLIBS) +XLFLAGS= $(XLIBS) $(FLIBS) + +LD = cc +LDFLAGS = -g + +# +# Shell to perform make commands +# +SHELL=/bin/sh + +# +# Sequence library handling routines +# +SEQLIB=\ + seqlibsubs.o +# /usr/lib/fixade.o + + +# +# The C objects, needed by every X program +# +CCORE=\ + postscript.o\ + Graph.o\ + plotLog.o\ + help.o\ + dialogues.o\ + userfacecom.o\ + helpnmenu.o\ + xmenu.o\ + FtoC.o\ + mcspec.o\ + locks.o + +COBJS=\ + main.o\ + $(CCORE) + +XDAPCOBJS=\ + xdapmain.o\ + $(CCORE) + + +# +# Common objects, needed by every program +# +COMMONOBJSB=\ + fmain.o\ + seeme-sg.o\ + nxspec.o\ + userface.o\ + userfacecom.o\ + nxhelpmenu.o\ + helpnmenu.o\ + FtoC.o\ + subs89.a + +COMMONOBJS=\ + $(COMMONOBJSB)\ + postscript.o + +SCOMMONOBJS=\ + $(COMMONOBJSB) + +XCOMMONOBJS=\ + seeme-sg.o\ + xspec.o\ + subs89.a\ + $(COBJS) + +XDAPCOMMONOBJS=\ + seeme-sg.o\ + xspec.o\ + subs89.a\ + $(XDAPCOBJS) + + +# +# Building the programs +# This should be just a linking phase because all of the object +# files and library files are generated using implicit rules. +# We use the fortran compiler to do linking. +# +GIP=\ + gip.o + +GIPOBJS=\ + $(GIP)\ + $(SCOMMONOBJS) + +gip: $(GIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(GIPOBJS) $(LFLAGS) + + +LIP=\ + lip.o + +LIPOBJS=\ + $(LIP)\ + $(SEQLIB)\ + $(COMMONOBJS) + +lip: $(LIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(LIPOBJS) $(LFLAGS) + + + + +MEP=\ + mep.o\ + mepsub.o\ + asubs89.a\ + plot92.a + +MEPOBJS=\ + $(MEP)\ + $(COMMONOBJS)\ + pl4010.o + +XMEPOBJS=\ + $(MEP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +mep: $(MEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(MEPOBJS) $(LFLAGS) + +xmep: $(XMEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XMEPOBJS) $(XLFLAGS) + + + + +NIP=\ + nip.o\ + patternn.a\ + patternnc.a\ + anals89.a\ + asubs89.a\ + plot92.a \ + $(SEQLIB) + +NIPOBJS=\ + $(NIP)\ + $(COMMONOBJS)\ + pl4010.o + +XNIPOBJS=\ + $(NIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +nip: $(NIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPOBJS) $(LFLAGS) + +xnip: $(XNIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XNIPOBJS) $(XLFLAGS) + + + + +NIPL=\ + nipl.o\ + patternnc.a\ + anals89.a\ + asubs89.a\ + $(SEQLIB) + +NIPLOBJS=\ + $(NIPL)\ + $(SCOMMONOBJS) + +nipl: $(NIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPLOBJS) $(LFLAGS) + + + +NIPF=\ + nipf.o\ + asubs89.a\ + plot92.a + +NIPFOBJS=\ + $(NIPF)\ + $(COMMONOBJS)\ + pl4010.o + + +nipf: $(NIPFOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPFOBJS) $(LFLAGS) + + + + + +PIP=\ + pip.o\ + analps89.a\ + patternp.a\ + patternpc.a\ + asubs89.a\ + plot92.a\ + $(SEQLIB) + +PIPOBJS=\ + $(PIP)\ + $(COMMONOBJS)\ + pl4010.o + +XPIPOBJS=\ + $(PIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +pip: $(PIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(PIPOBJS) $(LFLAGS) + +xpip:$(XPIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XPIPOBJS) $(XLFLAGS) + + + + +PIPL=\ + pipl.o\ + patternpc.a\ + analps89.a\ + asubs89.a\ + $(SEQLIB) + +PIPLOBJS=\ + $(PIPL)\ + $(SCOMMONOBJS) + +pipl: $(PIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(PIPLOBJS) $(LFLAGS) + + + +# +# Trace manager objects +# +STDTEDFILES=\ + seq.o\ + seqIOABI.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqIOPlain.o\ + opp.o\ + fpoint.o\ + mach-io.o + +TMANOBJS=\ + tman_main.o\ + tman_display.o\ + tman_traceDisplay.o\ + tman_basesDisplay.o\ + tman_context.o\ + tman_gadgets.o\ + $(STDTEDFILES) + +# Some versions of X11R4 may have a bug in SmeLine.c +XHACK=\ + SmeLine.o + +EDITOR=\ + $(XHACK)\ + xsapConEdit.o\ + contigEditor.o\ + edUtils.o\ + undo.o\ + Sheet.o\ + select.o\ + extend.o\ + searchUtils.o\ + edMenu.o\ + trans.o\ + $(MISCBIN)/misc.a + +TAGEDITOR=\ + tagEditor.o\ + tagdbparse.o\ + tagU2.o\ + tagU1.o + +DAP=\ + dap.o\ + dbsysnew.o\ + dbsyscommon.o\ + asubs89.a\ + plot92.a + +DAPOBJS=\ + $(DAP)\ + $(COMMONOBJS)\ + pl4010.o\ + conEdit.o\ + tagU2.o + +XDAPOBJS=\ + $(DAP)\ + $(XDAPCOMMONOBJS)\ + plX.o\ + textOutput.o\ + $(EDITOR)\ + $(TMANOBJS)\ + $(TAGEDITOR) + +dap: $(DAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(DAPOBJS) $(LFLAGS) + +xdap: $(XDAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XDAPOBJS) $(XLFLAGS) + +convert_project: cvt.o + $(CC) $(CFLAGS) -o $@ cvt.o + +SAP=\ + sap.o\ + dbsyscommon.o\ + dbsysold.o\ + asubs89.a\ + plot92.a + +SAPOBJS=\ + $(SAP)\ + $(COMMONOBJS)\ + pl4010.o + +XSAPOBJS=\ + $(SAP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +sap: $(SAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SAPOBJS) $(LFLAGS) + +xsap: $(XSAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XSAPOBJS) $(XLFLAGS) + + + + +SAPF=\ + sapf.o\ + dbsyscommon.o\ + dbsysold.o\ + asubs89.a\ + plot92.a + +SAPFOBJS=\ + $(SAPF)\ + sapmen.o\ + $(COMMONOBJS)\ + pl4010.o + +sapf: $(SAPFOBJS) + $(LD) $(LDFLAGS) -o $@ $(SAPFOBJS) $(LFLAGS) + + + + + + +SIP=\ + sip.o\ + dias89.a\ + plot92.a\ + $(SEQLIB) + +SIPOBJS=\ + $(SIP)\ + $(COMMONOBJS)\ + pl4010.o + +XSIPOBJS=\ + $(SIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +sip: $(SIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SIPOBJS) $(LFLAGS) + +xsip: $(XSIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XSIPOBJS) $(XLFLAGS) + + + + +SIPL=\ + sipl.o\ + dias89.a\ + $(SEQLIB) + +SIPLOBJS=\ + $(SIPL)\ + $(SCOMMONOBJS) + +sipl: $(SIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(SIPLOBJS) $(LFLAGS) + + + + +SETHELP=\ + sethelp.o + +SETHELPOBJS=\ + $(SETHELP)\ + +sethelp: $(SETHELPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SETHELPOBJS) $(LFLAGS) + + + + +SPLITP1=\ + splitp1.o +SPLITP1OBJS=\ + $(SPLITP1)\ + $(SCOMMONOBJS) + +splitp1: $(SPLITP1OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP1OBJS) $(LFLAGS) + + + + +SPLITP2=\ + splitp2.o +SPLITP2OBJS=\ + $(SPLITP2)\ + $(SCOMMONOBJS) + +splitp2: $(SPLITP2OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP2OBJS) $(LFLAGS) + + + + + +SPLITP3=\ + splitp3.o +SPLITP3OBJS=\ + $(SPLITP3)\ + $(SCOMMONOBJS) + +splitp3: $(SPLITP3OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP3OBJS) $(LFLAGS) + + + +REP=\ + rep.o\ + dias89.a\ + subs89.a + +REPOBJS=\ + $(REP)\ + $(COMMONOBJS) + +rep: $(REPOBJS) + $(F77) $(LDFLAGS) -o $@ $(REPOBJS) $(LFLAGS) + + + + + +VEP=\ + vep.o\ + dias89.a\ + subs89.a + + +VEPOBJS=\ + $(VEP)\ + $(SCOMMONOBJS) + + +vep: $(VEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPOBJS) $(LFLAGS) + + + + +# +# Special dependencies +# +# + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o: %.c + $(CC) $(CFLAGS) -c $< +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< +%.o: $(TEDSRC)/%.c + $(CC) $(CFLAGS) -c $< + +# Files requiring simple FORTRAN compilation (thing.f -> thing.o) +# +%.o: $(SRC)/%.f + $(F77) $(FFLAGS) -c $< + + +# +# The UNIX library (archive) mechanism is fairly weak so the following +# procedure is used to generate libraries. +# * split the source file up into files which each contain +# a single function or subroutine +# * compile each of those files +# * insert the object files individually into the library +# Each library is built by using a temporary directory. +# The fortran compilations must be done sequentially in order to +# avoid filling up the (small on this machine) /tmp directory +# with compiler debugging information. +# +%.a: $(SRC)/%.f + BINDIR=`pwd`; \ + cd $(SRC); \ + SRCDIR=`pwd`; \ + TMPDIR=/tmp/staden$$$$; \ + if test ! -d $$TMPDIR; \ + then mkdir $$TMPDIR; \ + fi; \ + cd $$TMPDIR; \ + rm -f *.f *.o; \ + fsplit $$SRCDIR/`expr $< : '.*/\(.*\)' \| $<`; \ + $(F77) $(FFLAGS) -c *.f; \ + rm *.f; \ + rm -f $$BINDIR/$@; \ + ar rcv $$BINDIR/$@ *.o; \ + echo ranlib $$BINDIR/$@; \ + rm *.o; \ + cd $$BINDIR; \ + rm -r $$TMPDIR + + + + + + +all: $(PROGS) + +nprogs: $(NPROGS) + +xprogs: $(XPROGS) + +lprogs: $(LPROGS) + +clean: + rm -f $(PROGS) *.a *.o *~ + + + diff --git a/src/staden/makefile-solaris b/src/staden/makefile-solaris new file mode 100644 index 0000000..3e91b59 --- /dev/null +++ b/src/staden/makefile-solaris @@ -0,0 +1,617 @@ +# Makefile for staden X programs (Solaris 2.1 version) +# ---------------------------------------------------- + +# This makefile generates the following staden programs which +# run under xterm (normal, `N') and those which run directly under X. +NPROGS= mep nip pip dap sap sip splitp1 splitp2 splitp3 gip sethelp convert_project rep lip sapf nipf vep +LPROGS= nipl pipl sipl +XPROGS= xmep xnip xpip xdap xsap xsip +PROGS= $(NPROGS) $(LPROGS) $(XPROGS) + + + + + + + +# +# ``Interesting'' suffices that make needs to know about +# +.SUFFIXES: .a $(SUFFIXES) + + +# +# Where the wild things are +# +SRC=. +TEDSRC=$(SRC)/../ted +MISCSRC=$(SRC)/../Misc +MISCBIN=$(MISCSRC) +OPENWININC=/usr/openwin/include +INCLUDES= -I$(SRC) -I$(TEDSRC) -I$(MISCSRC) -I$(OPENWININC) + + +# +# Compilers and flags +# +CC= gcc +GCCWARNINGS = -Wreturn-type -Wunused -Wswitch -Wcomment -Wformat +CFLAGS= -g -ansi -pedantic $(GCCWARNINGS) $(INCLUDES) +F77= f77 +FFLAGS= -ansi -g -C -Nx500 + +# +# Linking flags +# +# +#LD= ld +#LDFLAGS=-t $(LIBPATH) -Bstatic \ +# $(SPRO)/crti.o $(SPRO)/crt1.o $(SPRO)/cg89/__fstd.o \ +# $(SPRO)/values-xi.o -Y P,$(SPRO)/../lib:$(SPRO)/:/usr/ccs/lib:/usr/lib +# +#SPRO= /opt/SUNWspro/SC2.0.1 +#LIBPATH=-L$(SPRO) -L/usr/ccs/lib -L/usr/ucblib +#XLIBS= -lXaw -lXt -lXmu -lXext -lX11 +#FLIBS= -lV77 -lF77 -lM77 -lm +#LDOBJ= $(SPRO)/crtn.o +#LIBS= -lm -lc -lsocket -lnsl -lintl -Bdynamic -ldl -Bstatic -lucb -lelf \ +# $(LDOBJ) +#LFLAGS= $(FLIBS) $(LIBS) +#XLFLAGS=$(FLIBS) $(XLIBS) $(LIBS) + +LD = f77 +SPRO = /opt/SUNWspro/SC2.0.1 +LDFLAGS = -Bstatic -g -L$(SPRO) -L/usr/ccs/lib -L/usr/ucblib -L/usr/openwin/lib +LIBS = -lm -lucb -lelf -Bdynamic -ldl -Bstatic -lsocket -lnsl -lintl +FLIBS = -lV77 -lF77 -lM77 +XLIBS = -lXaw -lXt -lXmu -lXext -lX11 +LFLAGS = $(FLIBS) $(LIBS) +XLFLAGS = $(FLIBS) $(XLIBS) $(LIBS) + +# +# Sequence library handling routines +# +SEQLIB=\ + seqlibsubs.o + +# +# The C objects, needed by every X program +# +CCORE=\ + postscript.o\ + Graph.o\ + plotLog.o\ + help.o\ + dialogues.o\ + userfacecom.o\ + helpnmenu.o\ + xmenu.o\ + FtoC.o\ + mcspec.o\ + locks.o + +COBJS=\ + main.o\ + $(CCORE) + +XDAPCOBJS=\ + xdapmain.o\ + $(CCORE) + + +# +# Common objects, needed by every program +# +COMMONOBJSB=\ + fmain.o\ + seeme.o\ + nxspec.o\ + userface.o\ + userfacecom.o\ + nxhelpmenu.o\ + helpnmenu.o\ + FtoC.o\ + subs89.a + +COMMONOBJS=\ + $(COMMONOBJSB)\ + postscript.o + +SCOMMONOBJS=\ + $(COMMONOBJSB) + +XCOMMONOBJS=\ + seeme.o\ + xspec.o\ + subs89.a\ + $(COBJS) + +XDAPCOMMONOBJS=\ + seeme.o\ + xspec.o\ + subs89.a\ + $(XDAPCOBJS) + + +# +# Building the programs +# This should be just a linking phase because all of the object +# files and library files are generated using implicit rules. +# We use the fortran compiler to do linking. +# +GIP=\ + gip.o + +GIPOBJS=\ + $(GIP)\ + $(SCOMMONOBJS) + +gip: $(GIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(GIPOBJS) $(LFLAGS) + + +LIP=\ + lip.o + +LIPOBJS=\ + $(LIP)\ + $(SEQLIB)\ + $(COMMONOBJS) + +lip: $(LIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(LIPOBJS) $(LFLAGS) + + + + +MEP=\ + mep.o\ + mepsub.o\ + asubs89.a\ + plot92.a + +MEPOBJS=\ + $(MEP)\ + $(COMMONOBJS)\ + pl4010.o + +XMEPOBJS=\ + $(MEP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +mep: $(MEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(MEPOBJS) $(LFLAGS) + +xmep: $(XMEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XMEPOBJS) $(XLFLAGS) + + + + +NIP=\ + nip.o\ + patternn.a\ + patternnc.a\ + anals89.a\ + asubs89.a\ + plot92.a $(SEQLIB) + +NIPOBJS=\ + $(NIP)\ + $(COMMONOBJS)\ + pl4010.o + +XNIPOBJS=\ + $(NIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +nip: $(NIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPOBJS) $(LFLAGS) + +xnip: $(XNIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XNIPOBJS) $(XLFLAGS) + + + + +NIPL=\ + nipl.o\ + patternnc.a\ + anals89.a\ + asubs89.a\ + $(SEQLIB) + +NIPLOBJS=\ + $(NIPL)\ + $(SCOMMONOBJS) + +nipl: $(NIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPLOBJS) $(LFLAGS) + + + +NIPF=\ + nipf.o\ + asubs89.a\ + plot92.a + +NIPFOBJS=\ + $(NIPF)\ + $(COMMONOBJS)\ + pl4010.o + + +nipf: $(NIPFOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPFOBJS) $(LFLAGS) + + + + + +PIP=\ + pip.o\ + analps89.a\ + patternp.a\ + patternpc.a\ + asubs89.a\ + plot92.a\ + $(SEQLIB) + +PIPOBJS=\ + $(PIP)\ + $(COMMONOBJS)\ + pl4010.o + +XPIPOBJS=\ + $(PIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +pip: $(PIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(PIPOBJS) $(LFLAGS) + +xpip:$(XPIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XPIPOBJS) $(XLFLAGS) + + + + +PIPL=\ + pipl.o\ + patternpc.a\ + analps89.a\ + asubs89.a\ + $(SEQLIB) + +PIPLOBJS=\ + $(PIPL)\ + $(SCOMMONOBJS) + +pipl: $(PIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(PIPLOBJS) $(LFLAGS) + + + +# +# Trace manager objects +# +STDTEDFILES=\ + seq.o\ + seqIOABI.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqIOPlain.o\ + opp.o\ + fpoint.o\ + mach-io.o + +TMANOBJS=\ + tman_main.o\ + tman_display.o\ + tman_traceDisplay.o\ + tman_basesDisplay.o\ + tman_context.o\ + tman_gadgets.o\ + $(STDTEDFILES) + +# Some versions of X11R4 may have a bug in SmeLine.c +XHACK=\ + SmeLine.o + +EDITOR=\ + $(XHACK)\ + xsapConEdit.o\ + contigEditor.o\ + edUtils.o\ + undo.o\ + Sheet.o\ + select.o\ + extend.o\ + searchUtils.o\ + edMenu.o\ + trans.o\ + $(MISCBIN)/misc.a + +TAGEDITOR=\ + tagEditor.o\ + tagdbparse.o\ + tagU2.o\ + tagU1.o + +DAP=\ + dap.o\ + dbsysnew.o\ + dbsyscommon.o\ + asubs89.a\ + plot92.a + +DAPOBJS=\ + $(DAP)\ + $(COMMONOBJS)\ + pl4010.o\ + conEdit.o\ + tagU2.o + +XDAPOBJS=\ + $(DAP)\ + $(XDAPCOMMONOBJS)\ + plX.o\ + textOutput.o\ + $(EDITOR)\ + $(TMANOBJS)\ + $(TAGEDITOR) + +dap: $(DAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(DAPOBJS) $(LFLAGS) + +xdap: $(XDAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XDAPOBJS) $(XLFLAGS) + +convert_project: cvt.o + $(CC) $(CFLAGS) -o $@ cvt.o + +SAP=\ + sap.o\ + dbsyscommon.o\ + dbsysold.o\ + asubs89.a\ + plot92.a + +SAPOBJS=\ + $(SAP)\ + $(COMMONOBJS)\ + pl4010.o + +XSAPOBJS=\ + $(SAP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +sap: $(SAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SAPOBJS) $(LFLAGS) + +xsap: $(XSAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XSAPOBJS) $(XLFLAGS) + + + + +SAPF=\ + sapf.o\ + dbsyscommon.o\ + dbsysold.o\ + asubs89.a\ + plot92.a + +SAPFOBJS=\ + $(SAPF)\ + sapmen.o\ + $(COMMONOBJS)\ + pl4010.o + +sapf: $(SAPFOBJS) + $(LD) $(LDFLAGS) -o $@ $(SAPFOBJS) $(LFLAGS) + + + + + + +SIP=\ + sip.o\ + dias89.a\ + plot92.a\ + $(SEQLIB) + +SIPOBJS=\ + $(SIP)\ + $(COMMONOBJS)\ + pl4010.o + +XSIPOBJS=\ + $(SIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +sip: $(SIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SIPOBJS) $(LFLAGS) + +xsip: $(XSIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XSIPOBJS) $(XLFLAGS) + + + + +SIPL=\ + sipl.o\ + dias89.a\ + $(SEQLIB) + +SIPLOBJS=\ + $(SIPL)\ + $(SCOMMONOBJS) + +sipl: $(SIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(SIPLOBJS) $(LFLAGS) + + + + +SETHELP=\ + sethelp.o + +SETHELPOBJS=\ + $(SETHELP) + +sethelp: $(SETHELPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SETHELPOBJS) $(LFLAGS) + + + + +SPLITP1=\ + splitp1.o +SPLITP1OBJS=\ + $(SPLITP1)\ + $(SCOMMONOBJS) + +splitp1: $(SPLITP1OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP1OBJS) $(LFLAGS) + + + + +SPLITP2=\ + splitp2.o +SPLITP2OBJS=\ + $(SPLITP2)\ + $(SCOMMONOBJS) + +splitp2: $(SPLITP2OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP2OBJS) $(LFLAGS) + + + + + +SPLITP3=\ + splitp3.o +SPLITP3OBJS=\ + $(SPLITP3)\ + $(SCOMMONOBJS) + +splitp3: $(SPLITP3OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP3OBJS) $(LFLAGS) + + + +REP=\ + rep.o\ + dias89.a\ + subs89.a + +REPOBJS=\ + $(REP)\ + $(COMMONOBJS) + +rep: $(REPOBJS) + $(F77) $(LDFLAGS) -o $@ $(REPOBJS) $(LFLAGS) + + + + + +VEP=\ + vep.o\ + dias89.a\ + subs89.a + + +VEPOBJS=\ + $(VEP)\ + $(SCOMMONOBJS) + + +vep: $(VEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPOBJS) $(LFLAGS) + + + + +# +# Special dependencies +# +# + + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o: %.c + $(CC) $(CFLAGS) -c $< +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< +%.o: $(TEDSRC)/%.c + $(CC) $(CFLAGS) -c $< + +# Files requiring simple FORTRAN compilation (thing.f -> thing.o) +# +%.o: $(SRC)/%.f + $(F77) $(FFLAGS) -c $< + + + +# +# The UNIX library (archive) mechanism is fairly weak so the following +# procedure is used to generate libraries. +# * split the source file up into files which each contain +# a single function or subroutine +# * compile each of those files +# * insert the object files individually into the library +# Each library is built by using a temporary directory. +# The fortran compilations must be done sequentially in order to +# avoid filling up the (small on this machine) /tmp directory +# with compiler debugging information. +# +%.a: $(SRC)/%.f + BINDIR=`pwd`; \ + cd $(SRC); \ + SRCDIR=`pwd`; \ + TMPDIR=/tmp/staden$$$$; \ + if test ! -d $$TMPDIR; \ + then mkdir $$TMPDIR; \ + fi; \ + cd $$TMPDIR; \ + rm -f *.f *.o; \ + fsplit $$SRCDIR/`expr $< : '.*/\(.*\)' \| $<`; \ + $(F77) $(FFLAGS) -c *.f; \ + rm *.f; \ + rm -f $$BINDIR/$@; \ + ar rcv $$BINDIR/$@ *.o; \ + rm *.o; \ + cd $$BINDIR; \ + rm -r $$TMPDIR + + + + + + +all: $(PROGS) + +nprogs: $(NPROGS) + +xprogs: $(XPROGS) + +lprogs: $(LPROGS) + +clean: + rm -f $(PROGS) *.a *.o *~ + diff --git a/src/staden/makefile-sun b/src/staden/makefile-sun new file mode 100644 index 0000000..82262fd --- /dev/null +++ b/src/staden/makefile-sun @@ -0,0 +1,602 @@ +# Makefile for staden X programs (Sun version) +# -------------------------------------------- + +# This makefile generates the following staden programs which +# run under xterm (normal, `N') and those which run directly under X. +NPROGS= mep nip pip dap sap sip splitp1 splitp2 splitp3 gip sethelp convert_project rep lip sapf nipf vep +LPROGS= nipl pipl sipl +XPROGS= xmep xnip xpip xdap xsap xsip +PROGS= $(NPROGS) $(LPROGS) $(XPROGS) + + + + + + + +# +# ``Interesting'' suffices that make needs to know about +# +.SUFFIXES: .a $(SUFFIXES) + + +# +# Where the wild things are +# +SRC=. +TEDSRC=$(SRC)/../ted +MISCSRC=$(SRC)/../Misc +MISCBIN=$(MISCSRC) +INCLUDES= -I$(SRC) -I$(TEDSRC) -I$(MISCSRC) + + +# +# Compilers and flags +# +CC= gcc +GCCWARNINGS = -Wreturn-type -Wunused -Wswitch -Wcomment -W -Wformat \ + -Wuninitialized +CFLAGS= -g -ansi -pedantic $(GCCWARNINGS) $(INCLUDES) +# KFLAGS - for compiling non ANSI C modules +KFLAGS= -g $(INCLUDES) +F77= f77 +FFLAGS= -ansi -g -C -Nx500 + +XLIBS= -lXaw -lXt -lXmu -lXext -lX11 +# cc -Bstatic, gcc-2.x -static, else nothing +LFLAGS= -static -lF77 -lI77 -lU77 -lm +XLFLAGS= $(LFLAGS) $(XLIBS) + +#LD=cc +# If using gcc-2.x, better link with it +LD=gcc +LDFLAGS= -g +# Alternative for those with problems with libraries +#XLIBS= -lXaw -lXt -lXmu -lXext -lX11 +#LFLAGS= -Bstatic +#XLFLAGS= $(LFLAGS) $(XLIBS) +#LD=f77 +#LDFLAGS= -g + +# +# Sequence library handling routines +# +SEQLIB=\ + seqlibsubs.o + +# +# The C objects, needed by every X program +# +CCORE=\ + postscript.o\ + Graph.o\ + plotLog.o\ + help.o\ + dialogues.o\ + userfacecom.o\ + helpnmenu.o\ + xmenu.o\ + FtoC.o\ + mcspec.o\ + locks.o + +COBJS=\ + main.o\ + $(CCORE) + +XDAPCOBJS=\ + xdapmain.o\ + $(CCORE) + + +# +# Common objects, needed by every program +# +COMMONOBJSB=\ + fmain.o\ + seeme.o\ + nxspec.o\ + userface.o\ + userfacecom.o\ + nxhelpmenu.o\ + helpnmenu.o\ + FtoC.o\ + subs89.a + +COMMONOBJS=\ + $(COMMONOBJSB)\ + postscript.o + +SCOMMONOBJS=\ + $(COMMONOBJSB) + +XCOMMONOBJS=\ + seeme.o\ + xspec.o\ + subs89.a\ + $(COBJS) + +XDAPCOMMONOBJS=\ + seeme.o\ + xspec.o\ + subs89.a\ + $(XDAPCOBJS) + + +# +# Building the programs +# This should be just a linking phase because all of the object +# files and library files are generated using implicit rules. +# We use the fortran compiler to do linking. +# +GIP=\ + gip.o + +GIPOBJS=\ + $(GIP)\ + $(SCOMMONOBJS) + +gip: $(GIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(GIPOBJS) $(LFLAGS) + + +LIP=\ + lip.o + +LIPOBJS=\ + $(LIP)\ + $(SEQLIB)\ + $(COMMONOBJS) + +lip: $(LIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(LIPOBJS) $(LFLAGS) + + +MEP=\ + mep.o\ + mepsub.o\ + asubs89.a\ + plot92.a + +MEPOBJS=\ + $(MEP)\ + $(COMMONOBJS)\ + pl4010.o + +XMEPOBJS=\ + $(MEP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +mep: $(MEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(MEPOBJS) $(LFLAGS) + +xmep: $(XMEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XMEPOBJS) $(XLFLAGS) + + + + +NIP=\ + nip.o\ + patternn.a\ + patternnc.a\ + anals89.a\ + asubs89.a\ + plot92.a $(SEQLIB) + +NIPOBJS=\ + $(NIP)\ + $(COMMONOBJS)\ + pl4010.o + +XNIPOBJS=\ + $(NIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +nip: $(NIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPOBJS) $(LFLAGS) + +xnip: $(XNIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XNIPOBJS) $(XLFLAGS) + + + + +NIPL=\ + nipl.o\ + patternnc.a\ + anals89.a\ + asubs89.a\ + $(SEQLIB) + +NIPLOBJS=\ + $(NIPL)\ + $(SCOMMONOBJS) + +nipl: $(NIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPLOBJS) $(LFLAGS) + + + +NIPF=\ + nipf.o\ + asubs89.a\ + plot92.a + +NIPFOBJS=\ + $(NIPF)\ + $(COMMONOBJS)\ + pl4010.o + + +nipf: $(NIPFOBJS) + $(LD) $(LDFLAGS) -o $@ $(NIPFOBJS) $(LFLAGS) + + + + + +PIP=\ + pip.o\ + analps89.a\ + patternp.a\ + patternpc.a\ + asubs89.a\ + plot92.a\ + $(SEQLIB) + +PIPOBJS=\ + $(PIP)\ + $(COMMONOBJS)\ + pl4010.o + +XPIPOBJS=\ + $(PIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +pip: $(PIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(PIPOBJS) $(LFLAGS) + +xpip:$(XPIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XPIPOBJS) $(XLFLAGS) + + + + +PIPL=\ + pipl.o\ + patternpc.a\ + analps89.a\ + asubs89.a\ + $(SEQLIB) + +PIPLOBJS=\ + $(PIPL)\ + $(SCOMMONOBJS) + +pipl: $(PIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(PIPLOBJS) $(LFLAGS) + + + +# +# Trace manager objects +# +STDTEDFILES=\ + seq.o\ + seqIOABI.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqIOPlain.o\ + opp.o\ + fpoint.o\ + mach-io.o + +TMANOBJS=\ + tman_main.o\ + tman_display.o\ + tman_traceDisplay.o\ + tman_basesDisplay.o\ + tman_context.o\ + tman_gadgets.o\ + $(STDTEDFILES) + +# Some versions of X11R4 may have a bug in SmeLine.c +XHACK=\ + SmeLine.o + +EDITOR=\ + $(XHACK)\ + xsapConEdit.o\ + contigEditor.o\ + edUtils.o\ + undo.o\ + Sheet.o\ + select.o\ + extend.o\ + searchUtils.o\ + edMenu.o\ + trans.o\ + $(MISCBIN)/misc.a + +TAGEDITOR=\ + tagEditor.o\ + tagdbparse.o\ + tagU2.o\ + tagU1.o + +DAP=\ + dap.o\ + dbsysnew.o\ + dbsyscommon.o\ + asubs89.a\ + plot92.a + +DAPOBJS=\ + $(DAP)\ + $(COMMONOBJS)\ + pl4010.o\ + conEdit.o\ + tagU2.o + +XDAPOBJS=\ + $(DAP)\ + $(XDAPCOMMONOBJS)\ + plX.o\ + textOutput.o\ + $(EDITOR)\ + $(TMANOBJS)\ + $(TAGEDITOR) + +dap: $(DAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(DAPOBJS) $(LFLAGS) + +xdap: $(XDAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XDAPOBJS) $(XLFLAGS) + +convert_project: cvt.o + $(CC) $(CFLAGS) -o $@ cvt.o + +SAP=\ + sap.o\ + dbsyscommon.o\ + dbsysold.o\ + asubs89.a\ + plot92.a + +SAPOBJS=\ + $(SAP)\ + $(COMMONOBJS)\ + pl4010.o + +XSAPOBJS=\ + $(SAP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +sap: $(SAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SAPOBJS) $(LFLAGS) + +xsap: $(XSAPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XSAPOBJS) $(XLFLAGS) + + + + +SAPF=\ + sapf.o\ + dbsyscommon.o\ + dbsysold.o\ + asubs89.a\ + plot92.a + +SAPFOBJS=\ + $(SAPF)\ + sapmen.o\ + $(COMMONOBJS)\ + pl4010.o + +sapf: $(SAPFOBJS) + $(LD) $(LDFLAGS) -o $@ $(SAPFOBJS) $(LFLAGS) + + + + + + +SIP=\ + sip.o\ + dias89.a\ + plot92.a\ + $(SEQLIB) + +SIPOBJS=\ + $(SIP)\ + $(COMMONOBJS)\ + pl4010.o + +XSIPOBJS=\ + $(SIP)\ + $(XCOMMONOBJS)\ + plX.o\ + textOutput.o + +sip: $(SIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SIPOBJS) $(LFLAGS) + +xsip: $(XSIPOBJS) + $(LD) $(LDFLAGS) -o $@ $(XSIPOBJS) $(XLFLAGS) + + + + +SIPL=\ + sipl.o\ + dias89.a\ + $(SEQLIB) + +SIPLOBJS=\ + $(SIPL)\ + $(SCOMMONOBJS) + +sipl: $(SIPLOBJS) + $(LD) $(LDFLAGS) -o $@ $(SIPLOBJS) $(LFLAGS) + + + + +SETHELP=\ + sethelp.o + +SETHELPOBJS=\ + $(SETHELP) + +sethelp: $(SETHELPOBJS) + $(LD) $(LDFLAGS) -o $@ $(SETHELPOBJS) $(LFLAGS) + + + + +SPLITP1=\ + splitp1.o +SPLITP1OBJS=\ + $(SPLITP1)\ + $(SCOMMONOBJS) + +splitp1: $(SPLITP1OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP1OBJS) $(LFLAGS) + + + + +SPLITP2=\ + splitp2.o +SPLITP2OBJS=\ + $(SPLITP2)\ + $(SCOMMONOBJS) + +splitp2: $(SPLITP2OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP2OBJS) $(LFLAGS) + + + + + +SPLITP3=\ + splitp3.o +SPLITP3OBJS=\ + $(SPLITP3)\ + $(SCOMMONOBJS) + +splitp3: $(SPLITP3OBJS) + $(LD) $(LDFLAGS) -o $@ $(SPLITP3OBJS) $(LFLAGS) + +REP=\ + rep.o\ + dias89.a\ + subs89.a + +REPOBJS=\ + $(REP)\ + $(COMMONOBJS) + +rep: $(REPOBJS) + $(LD) $(LDFLAGS) -o $@ $(REPOBJS) $(LFLAGS) + + + +VEP=\ + vep.o\ + dias89.a\ + subs89.a + + +VEPOBJS=\ + $(VEP)\ + $(SCOMMONOBJS) + + +vep: $(VEPOBJS) + $(LD) $(LDFLAGS) -o $@ $(VEPOBJS) $(LFLAGS) + + + + +# +# Special dependencies +# +# + + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o: %.c + $(CC) $(CFLAGS) -c $< +%.o: $(SRC)/%.c + $(CC) $(CFLAGS) -c $< +%.o: $(TEDSRC)/%.c + $(CC) $(CFLAGS) -c $< + +# Files requiring simple FORTRAN compilation (thing.f -> thing.o) +# +%.o: $(SRC)/%.f + $(F77) $(FFLAGS) -c $< + + + +# +# The UNIX library (archive) mechanism is fairly weak so the following +# procedure is used to generate libraries. +# * split the source file up into files which each contain +# a single function or subroutine +# * compile each of those files +# * insert the object files individually into the library +# Each library is built by using a temporary directory. +# The fortran compilations must be done sequentially in order to +# avoid filling up the (small on this machine) /tmp directory +# with compiler debugging information. +# +%.a: $(SRC)/%.f + BINDIR=`pwd`; \ + cd $(SRC); \ + SRCDIR=`pwd`; \ + TMPDIR=/tmp/staden$$$$; \ + if test ! -d $$TMPDIR; \ + then mkdir $$TMPDIR; \ + fi; \ + cd $$TMPDIR; \ + rm -f *.f *.o; \ + fsplit $$SRCDIR/`expr $< : '.*/\(.*\)' \| $<`; \ + $(F77) $(FFLAGS) -c *.f; \ + rm *.f; \ + rm -f $$BINDIR/$@; \ + ar rcv $$BINDIR/$@ *.o; \ + ranlib $$BINDIR/$@; \ + rm *.o; \ + cd $$BINDIR; \ + rm -r $$TMPDIR + + + + + + +all: $(PROGS) + +nprogs: $(NPROGS) + +xprogs: $(XPROGS) + +lprogs: $(LPROGS) + +clean: + rm -f $(PROGS) *.a *.o *~ + diff --git a/src/staden/mcspec.c b/src/staden/mcspec.c new file mode 100644 index 0000000..edee3ea --- /dev/null +++ b/src/staden/mcspec.c @@ -0,0 +1,42 @@ +/* Title: mcspec + + File: mcspec.c + Purpose: Machine, OS and language specific features + Last update: Mon Mar 19th 1990 +*/ + +#include "fort.h" +#include +#include + + +/* ---- Xlib includes ---- */ +#include /* IMPORT: XBell */ + + +/* ---- Application includes ---- */ +#include "mcspec.h" +#include "main.h" /* IMPORT: GetDisplay */ + + + + +void bell_(int_f *n_ptr, int_f *kbout_ptr) +/* Ring the bell `n' times +*/ +{ int_f i; + + for (i=1; i<=*n_ptr; i++) { + XBell(GetDisplay(), 100); + } +} + +#ifdef NOREMOVE +int remove(const char *filename) +/* This is an ANSI standard function. + It is implemented here because few UNIX systems support it. +*/ +{ + return(unlink(filename)); +} +#endif diff --git a/src/staden/mcspec.h b/src/staden/mcspec.h new file mode 100644 index 0000000..8471119 --- /dev/null +++ b/src/staden/mcspec.h @@ -0,0 +1,37 @@ +/* + Title: mcspec + + File: mcspec.h + Purpose: Machine, OS and language specific features + Last update: Mon Jun 10 1993 +*/ + +#ifndef _MCSPEC_H_ +#define _MCSPEC_H_ + + +#include "fort.h" + +extern void bell_(int_f *n_ptr, int_f *kbout_ptr); +/* + Ring the bell `n' times +*/ + + +extern int remove(const char *filename); +/* + This is an ANSI standard function. + It is implemented here because few UNIX systems support it. +*/ + + +extern char *getenv(const char *name); +/* + This is an ANSI standard function. + It's type is declared here becuase there is no stdlib.h + on this machine. +*/ + + + +#endif /*_MCSPEC_H_*/ diff --git a/src/staden/mep.f b/src/staden/mep.f new file mode 100644 index 0000000..5c1dd95 --- /dev/null +++ b/src/staden/mep.f @@ -0,0 +1,394 @@ +C author Rodger Staden +C 22-oct-1992 introduced mask +C 8-jul-1992 allowed words as short as 2 letters +C 16-mar-1990 removed escape after bpause +C 5-4 90 changed getdev to redir (see redir) +C 22-6-90 Added work for use by makeds (it had its own array +C but it was too small.) +C 14-11-90 replaced radio by radion +C 13-04-91 removed initial CLEARV + SUBROUTINE FMAIN() + PARAMETER ( MAXSEQ = 1000, + + MAXWRD = 8, + + IDM = 5, + + MAXDEV = 7, + + MAXDIC = (IDM-1)**MAXWRD, + + MAXLIS = 100, + + MAXCHR = 20000, + + MINMEN = -3, + + MAXMEN = 33, + + MAXMAS = 25) + CHARACTER SEQ(MAXCHR) + CHARACTER*60 FILNAM,HELPF,POINTF,FILMAR + PARAMETER ( HELPF = 'MEPHELP', + + POINTF = 'MEPHPNT', + + FILMAR = 'MEPMARG') + INTEGER SEQN(MAXCHR),DW(MAXDIC),DEVNOS(MAXDEV),DS(MAXDIC) + INTEGER LENSEQ(MAXSEQ),DM(MAXDIC),DT(MAXDIC),DH(MAXDIC) + INTEGER TEMPC(MAXLIS),TEMPP(MAXLIS),TEMPI(MAXLIS),TEMPN(MAXLIS) + INTEGER HELPS(0:MAXMEN),HELPE(0:MAXMEN),WT(4,MAXWRD) + INTEGER MARGB(MAXMEN),MARGT(MAXMEN),WORK(MAXCHR) + REAL COMP(5) + PARAMETER (MAXPRM = 27) + CHARACTER PROMPT(3)*(MAXPRM) + LOGICAL MASK(MAXMAS) + CALL INTHLP('mep', MAXMEN) + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + CALL OPENGR(DEVNOS(7)) + CALL INITLU(IDM) + WRITE(KBOUT,*)' MEP (Motif exploration program) V2.0 Oct 1992' + WRITE(KBOUT,*)' Author Rodger Staden' + IGORT = 0 + IDEV = KBOUT + IDEVH = DEVNOS(3) + IDEVO = DEVNOS(5) + CALL SETHLP(HELPS,HELPE,0,MAXMEN,POINTF,IDEVH,KBOUT) +C GET SCREEN AND MARGIN SIZES + CALL GETMRG(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + +MAXMEN,DEVNOS(2),FILMAR) + CALL INITGR(KBIN,KBOUT,HELPS(0),HELPE(0),HELPF,IDEVH) +1 CONTINUE + CALL RSEQ(DEVNOS(1),DEVNOS(2),FILNAM,SEQ,MAXCHR,MAXSEQ,LENSEQ, + +NFILE,LMAX,KBIN,KBOUT,HELPS(3),HELPE(3),HELPF,IDEVH,IOK) + IF(IOK.NE.0) STOP + CALL CONNUM(SEQ,SEQN,LENSEQ(NFILE)) + I1 = 1 + I2 = LMAX + XMIN = I1 + XMAX = I2 + YMIN = 1 + YMAX = NFILE - 1 + CALL GETCOM(SEQN,LENSEQ(NFILE),COMP) + ICOMP = 1 + KCOMP = 0 + LENGTH = 6 + LMASK = LENGTH + MAXPOS = (IDM-1)**LENGTH + NFUZ = 1 + IDICW = 1 + IDICM = 0 + IDICH = 0 + IDICS = 1 + DO 2 I=1,LMASK + MASK(I) = .TRUE. + 2 CONTINUE +10 CONTINUE + CALL VT100M + CALL BPAUSE(KBIN,KBOUT,IOK) +C IF(IOK.NE.0) GO TO 9999 + CALL MENU(NOPT,KOPT,MOPT,MAXMEN,MINMEN,KBIN,KBOUT, + +HELPS,HELPE,HELPF,IDEVH) + IF(NOPT.EQ.2) GO TO 9999 + IF(NOPT.EQ.3)GO TO 1 +C +C help +C + IF(NOPT.EQ.1) THEN + CALL HELP(HELPS,HELPE,0,MAXMEN,HELPF,IDEVH, + + KBIN,KBOUT) + GO TO 10 + END IF + IF(NOPT.EQ.4)THEN + MN = 1 + MX = LMAX + CALL GETINT(MN,MX,I1,'Start position', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + I1 = IVAL + MN = I1 + LENGTH - 1 + MX = LMAX + CALL GETINT(MN,MX,I2,'End position', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + I2 = IVAL + IDICW = 1 + IDICM = 0 + IDICH = 0 + IDICS = 1 + GO TO 10 + END IF + IF(NOPT.EQ.5) THEN + LINLEN = 50 + CALL LISTN(SEQ,MAXCHR,LENSEQ,NFILE,LMAX,LINLEN,I1,I2, + + IDEV,KBOUT) + END IF + IF(NOPT.EQ.6)THEN + CALL TTEXT(DEVNOS(4),FILNAM,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + GO TO 10 + END IF + IF(NOPT.EQ.7)THEN +C GET DISK OUTPUT FILE ON UNIT DEVNOS(2) IF REQUIRED + CALL REDIR(IDEV,DEVNOS(6),DEVNOS(7),IGORT,FILNAM,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + GO TO 10 + END IF + IF(NOPT.EQ.33)THEN + CALL HAIRPN(SEQ,MAXCHR,LENSEQ,NFILE,LMAX, + + DW,DM,DT,LMAX, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX, + + KBOUT,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + IDICW = 1 + IDICH = 0 + IDICM = 0 + GO TO 10 + END IF + IF(NOPT.EQ.17)THEN +C PLOT + IB = 1 + PROMPT(1) = 'Plot match positions' + PROMPT(2) = 'Plot histogram of matches' + CALL RADION('Select plot mode',PROMPT,2,IB, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH,KBIN,KBOUT) + IF(IB.LT.1) GO TO 10 + IF(IB.EQ.1) THEN + CALL PLOTP(SEQ,MAXCHR,LENSEQ,NFILE,DT,LMAX,KBIN,KBOUT, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX, + + I1,I2,IDEV, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + ELSE + CALL PLOTH(SEQ,MAXCHR,LENSEQ,NFILE,DT,LMAX,KBIN,KBOUT, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX,I1,I2, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + END IF + GO TO 10 + END IF +C XHAIRS + IF(NOPT.EQ.13)THEN + CALL CLEARV + CALL XHAIRS(XMAX,XMIN,YMAX,YMIN,MARGL,MARGR, + +MARGB(NOPT),MARGT(NOPT), + +ISXMAX,ISYMAX,IHX,IHY,N,KBOUT) + GO TO 10 + END IF +C RULER + IF(NOPT.EQ.12)THEN + WRITE(KBOUT,*)' Horizontal scale' + ISH1=XMIN + ISH2=XMAX + CALL RULER(ISH1,ISH2,MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + + ISXMAX,ISYMAX,KBIN,KBOUT,1, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(3)) + WRITE(KBOUT,*)' Vertical scale' + ISV1=YMIN + ISV2=YMAX + CALL RULER(ISV1,ISV2,MARGL,MARGR,MARGB(NOPT),MARGT(NOPT), + + ISXMAX,ISYMAX,KBIN,KBOUT,2, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(3)) + GO TO 10 + END IF +C CLEAR GRAPHICS + IF(NOPT.EQ.10)THEN + CALL CLEARG + GO TO 10 + END IF +C KLEAR TEXT + IF(NOPT.EQ.11)THEN + CALL CLEARV + GO TO 10 + END IF +C CHANGE MARGINS + IF(NOPT.EQ.14)THEN + CALL MARGC(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + + HELPS(NOPT),HELPE(NOPT),MAXMEN,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF +C LABEL + IF(NOPT.EQ.15)THEN + CALL LABLER(KBIN,KBOUT,ISXMAX,ISYMAX, + +HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(3)) + GO TO 10 + END IF + IF(NOPT.EQ.20)THEN +C MN = 2 +C MX = MAXWRD +C CALL GETINT(MN,MX,LENGTH,'Word length', +C + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH,IOK) +C IF(IOK.NE.0) GO TO 10 +C LENGTH = IVAL +C +C DEFINE A MASK +C + WRITE(KBOUT,*)'Define word as a mask' + LMASK = MAXMAS + LENGTH = MAXWRD + CALL GMASK(KBIN,KBOUT,MASK,LMASK,LENGTH, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + MAXPOS = (IDM-1)**LENGTH + IDICS = 1 + IDICW = 1 + IDICM = 0 + IDICH = 0 + ELSE IF (NOPT.EQ.22) THEN + CALL SHOWST(NFUZ,I1,I2,KCOMP,ICOMP,IDICW,IDICS, + + IDICM,IDICH,MASK,LMASK,KBOUT) + ELSE IF (NOPT.EQ.21)THEN + MN = 0 + MX = 2 + CALL GETINT(MN,MX,NFUZ,'Number of mismatches', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH,IOK) + IF(IOK.NE.0) GO TO 10 + NFUZ = IVAL + IDICW = 1 + IDICM = 0 + IDICH = 0 + ELSE IF (NOPT.EQ.18)THEN + IB = KCOMP + 1 + PROMPT(1) = 'Search input strand' + PROMPT(2) = 'Search complementary strand' + PROMPT(3) = 'Search both strands' + CALL RADION('Select search mode',PROMPT,3,IB, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH,KBIN,KBOUT) + IF(IB.LT.1) GO TO 10 + KCOMP = IB - 1 + CALL TCOMP(COMP,KCOMP) + ELSE IF (NOPT.EQ.19) THEN + CALL GETCM(COMP,KBIN,KBOUT,SEQN,LENSEQ(NFILE),ICOMP, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + ELSE IF (NOPT.EQ.23)THEN +C +C MAKE DW +C +C GENERATE THE DICTIONARY FOR THIS WORD LENGTH + CALL FILLI(DW,MAXPOS,0) + WRITE(KBOUT,*)'Making Dw' + CALL MAKEDW(SEQN,MAXCHR,DW,DT,MAXPOS,LENSEQ, + + MAXSEQ,NFILE,LENGTH,KCOMP,I1,I2,MASK,LMASK) + IDICW = 0 + ELSE IF (NOPT.EQ.24) THEN +C +C MAKE DS +C + WRITE(KBOUT,*)'Making Ds' + CALL FILLI(DS,MAXPOS,0) + CALL MAKEDS(SEQN,MAXCHR,DS,DT,MAXPOS,LENSEQ, + + MAXSEQ,NFILE,LENGTH,KCOMP,I1,I2,WORK,MASK,LMASK) + IDICS = 0 + ELSE IF (NOPT.EQ.25) THEN +C +C MAKE DM FROM DW +C + IF(IDICW.NE.0) THEN + WRITE(KBOUT,*)'Please make Dw' + ELSE + WRITE(KBOUT,*)'Making Dm from Dw with ',NFUZ,' mismatch' + CALL MAKEDM(DW,DM,LENGTH,NFUZ,MAXPOS) + IDICM = 1 + IDICH = 0 + END IF + ELSE IF (NOPT.EQ.26) THEN +C +C MAKE DM FROM DS +C + IF(IDICS.NE.0) THEN + WRITE(KBOUT,*)'Please make Ds' + ELSE + WRITE(KBOUT,*)'Making Dm from Ds with ',NFUZ,' mismatch' + CALL MAKEDM(DS,DM,LENGTH,NFUZ,MAXPOS) + IDICM = 2 + IDICH = 0 + END IF + ELSE IF (NOPT.EQ.27)THEN +C +C MAKE DH +C + IF(IDICM.EQ.0)THEN + WRITE(KBOUT,*)'Please make dictionary' + ELSE + IF(IDICM.NE.0)THEN + WRITE(KBOUT,*)'Making Dh' + CALL MAKEDH(DH,DM,LENGTH,NFUZ,MAXPOS) + IDICH = IDICM + END IF + END IF + ELSE IF (NOPT.EQ.28)THEN + IF(IDICM.EQ.0) THEN + WRITE(KBOUT,*)'Please make dictionary' + ELSE IF (IDICM.EQ.1) THEN + CALL EXAMDM(DM,DW, + + MAXPOS,LENGTH,KBIN,KBOUT,WT,NFUZ,IOK,COMP,NFILE,IDEV, + + TEMPC,TEMPI,TEMPP,TEMPN,MAXLIS,MASK,LMASK) + ELSE IF (IDICM.EQ.2) THEN + CALL EXAMDM(DM,DS, + + MAXPOS,LENGTH,KBIN,KBOUT,WT,NFUZ,IOK,COMP,NFILE,IDEV, + + TEMPC,TEMPI,TEMPP,TEMPN,MAXLIS,MASK,LMASK) + END IF + ELSE IF (NOPT.EQ.29)THEN + IF(IDICH.EQ.0) THEN + WRITE(KBOUT,*)'Please make dictionary' + ELSE IF (IDICH.EQ.1) THEN + CALL EXAMDM(DH,DW, + + MAXPOS,LENGTH,KBIN,KBOUT,WT,NFUZ,IOK,COMP,NFILE,IDEV, + + TEMPC,TEMPI,TEMPP,TEMPN,MAXLIS,MASK,LMASK) + ELSE IF (IDICH.EQ.2) THEN + CALL EXAMDM(DH,DS, + + MAXPOS,LENGTH,KBIN,KBOUT,WT,NFUZ,IOK,COMP,NFILE,IDEV, + + TEMPC,TEMPI,TEMPP,TEMPN,MAXLIS,MASK,LMASK) + END IF + ELSE IF (NOPT.EQ.30)THEN + IF(IDICM.EQ.0) THEN + WRITE(KBOUT,*)'Please make dictionary' + ELSE IF (IDICM.EQ.1) THEN + CALL EXAMDW(DM,DW, + + MAXPOS,LENGTH,KBIN,KBOUT,WT,NFUZ,IOK,COMP,NFILE,IDEV, + + TEMPC,TEMPI,TEMPP,TEMPN,MAXLIS,MASK,LMASK) + ELSE IF (IDICM.EQ.2) THEN + CALL EXAMDW(DM,DS, + + MAXPOS,LENGTH,KBIN,KBOUT,WT,NFUZ,IOK,COMP,NFILE,IDEV, + + TEMPC,TEMPI,TEMPP,TEMPN,MAXLIS,MASK,LMASK) + END IF + ELSE IF (NOPT.EQ.31)THEN + IF(IDICH.EQ.0) THEN + WRITE(KBOUT,*)'Please make dictionary' + ELSE IF (IDICH.EQ.1) THEN + CALL EXAMDW(DH,DW, + + MAXPOS,LENGTH,KBIN,KBOUT,WT,NFUZ,IOK,COMP,NFILE,IDEV, + + TEMPC,TEMPI,TEMPP,TEMPN,MAXLIS,MASK,LMASK) + ELSE IF (IDICH.EQ.2) THEN + CALL EXAMDW(DH,DS, + + MAXPOS,LENGTH,KBIN,KBOUT,WT,NFUZ,IOK,COMP,NFILE,IDEV, + + TEMPC,TEMPI,TEMPP,TEMPN,MAXLIS,MASK,LMASK) + END IF + ELSE IF (NOPT.EQ.32) THEN + CALL DICIO(KBIN,KBOUT,IOPT,IHELPS,IHELPE,HELPF,IDEVH) + IF(IOPT.EQ.1) THEN + CALL WDIC(IDEVO,FILNAM,DW,MAXPOS,KBIN,KBOUT,IOK, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + ELSE IF(IOPT.EQ.2) THEN + CALL WDIC(IDEVO,FILNAM,DS,MAXPOS,KBIN,KBOUT,IOK, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + ELSE IF(IOPT.EQ.3) THEN + CALL WDIC(IDEVO,FILNAM,DM,MAXPOS,KBIN,KBOUT,IOK, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + ELSE IF(IOPT.EQ.4) THEN + CALL WDIC(IDEVO,FILNAM,DH,MAXPOS,KBIN,KBOUT,IOK, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + ELSE IF(IOPT.EQ.5) THEN + CALL RDIC(IDEVO,FILNAM,DW,MAXPOS,KBIN,KBOUT,IOK, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + IF(IOK.EQ.0)IDICW = 0 +C IDICM = 1 +C IDICH = 1 + ELSE IF(IOPT.EQ.6) THEN + CALL RDIC(IDEVO,FILNAM,DS,MAXPOS,KBIN,KBOUT,IOK, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + IF(IOK.EQ.0)IDICS = 0 +C IDICH = 1 + ELSE IF(IOPT.EQ.7) THEN + WRITE(KBOUT,*)'Assuming from Dw' + CALL RDIC(IDEVO,FILNAM,DM,MAXPOS,KBIN,KBOUT,IOK, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + IF(IOK.EQ.0)IDICM = 1 + ELSE IF(IOPT.EQ.8) THEN + WRITE(KBOUT,*)'Assuming from Dw' + CALL RDIC(IDEVO,FILNAM,DH,MAXPOS,KBIN,KBOUT,IOK, + + HELPS(NOPT),HELPE(NOPT),HELPF,IDEVH) + IF(IOK.EQ.0)IDICH = 1 + END IF + END IF + GO TO 10 +9999 CONTINUE + CALL VT100M + CALL SHUTD + END diff --git a/src/staden/mepmen.f b/src/staden/mepmen.f new file mode 100644 index 0000000..5db4d9a --- /dev/null +++ b/src/staden/mepmen.f @@ -0,0 +1,81 @@ + SUBROUTINE MENU(OPT,KOPT,MOPT,MAXOPT,MINMEN,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + INTEGER IHELPS(0:MAXOPT),IHELPE(0:MAXOPT) + INTEGER OPT + CHARACTER HELPF*(*) +1 CONTINUE + CALL CLEARV + IF(MOPT.EQ.0) THEN + WRITE(KBOUT,1000) + 1000 FORMAT(' Menus and their numbers are',/, + +' m0 = This menu',/, + +' m1 = General menu',/, + +' m2 = Screen control menu',/, + +' m3 = Dictionary analysis menu',/, + +' ? = Help',/, + +' ! = Quit') + ELSE IF (MOPT.EQ.-1) THEN + WRITE(KBOUT,1001) +1001 FORMAT(' General menu',/, + + ' 0 = List of menus',/, + + ' ? = Help',/, + + ' ! = Quit'/, + + ' 3 = Read new sequences',/, + + ' 4 = Redefine active region',/, + + ' 5 = List the sequences',/, + + ' 6 = List text file',/, + + ' 7 = Direct output to disk',/, + + ' 17 = Search for strings',/, + + ' 33 = Find inverted repeats') + ELSE IF (MOPT.EQ.-2) THEN + WRITE(KBOUT,1002) +1002 FORMAT(' Screen control menu',/, + + ' 0 = List of menus',/, + + ' ? = Help',/, + + ' ! = Quit'/, + + ' 10 = Clear graphics',/, + + ' 11 = Clear text',/, + + ' 12 = Draw ruler',/, + + ' 13 = Use cross hair',/, + + ' 14 = Reset margins',/, + + ' 15 = Label diagram',/, + + ' 16 = Draw map') + ELSE IF (MOPT.EQ.-3) THEN + WRITE(KBOUT,1003) +1003 FORMAT(' Dictionary analysis menu',/, + + ' 18 = Set strand',/, + + ' 19 = Set composition',/, + + ' 20 = Set word mask',/, + + ' 21 = Set number of mismatches',/, + + ' 22 = Show settings',/, + + ' 23 = Make dictionary Dw',/, + + ' 24 = Make dictionary Ds',/, + + ' 25 = Make fuzzy dictionary Dm from Dw',/, + + ' 26 = Make fuzzy dictionary Dm from Ds',/, + + ' 27 = Make fuzzy dictionary Dh from Dm',/, + + ' 28 = Examine fuzzy dictionary Dm',/, + + ' 29 = Examine fuzzy dictionary Dh',/, + + ' 30 = Examine words in Dm',/, + + ' 31 = Examine words in Dh',/, + + ' 32 = Save or restore a dictionary') + END IF + WRITE(KBOUT,1004) +1004 FORMAT(' ? Menu or option number=',$) + CALL GETOPT(KBIN,KOPT,OPT) + IF((OPT.LT.MINMEN).OR.(OPT.GT.MAXOPT).OR.(KOPT.GT.2)) THEN + MOPT = 0 + GO TO 1 + END IF + IF((KOPT.EQ.2).AND.(OPT.GE.0)) THEN + CALL HELP2(IHELPS(OPT),IHELPE(OPT),HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(OPT.EQ.0) THEN + MOPT = 0 + GO TO 1 + END IF + IF(OPT.LT.0) THEN + MOPT = OPT + GO TO 1 + END IF + END diff --git a/src/staden/mepsub.f b/src/staden/mepsub.f new file mode 100644 index 0000000..d76cbff --- /dev/null +++ b/src/staden/mepsub.f @@ -0,0 +1,1614 @@ +C Subroutines for program MEP +C author Rodger Staden +C 22-jun-1990 changed makeds to send work +C 9-7-90 removed menu routine +C 14-11-90 Replaced all radio by radion +C 2-3-92 set filnam = ' ' for some calls to openf1 +C +C CCODE +C DCODE +C DCODEI +C DICIO +C DRAWPN +C EXAMDM +C EXAMDW +C GETCM +C GETCOM +C GETV +C HAIRPD +C HAIRPN +C HARPN +C KTONUM +C LISTN +C LWRAP2 +C MAKEDH +C MAKEDM +C MAKEDS +C MAKEDW +C MENU +C NCODE +C NCODES +C OUTP +C PLOTH +C PLOTP +C RDDIC +C RDIC +C RINF +C RSEQ +C RSEQ1 +C RSEQ2 +C SHLSRT +C SHOWST +C SQFIT7 +C SQFIT8 +C STRNIN +C TCOMP +C WDCODE +C WDIC +C WFUZ2 +C WRTDIC +C WRTWMT +C WTMCON +C + SUBROUTINE RSEQ(IDEV1,IDEV2,FILNAM,SEQ,MAXCHR,MAXSEQ,LENSEQ, + +NFILE,LMAX,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + INTEGER LENSEQ(MAXSEQ) + CHARACTER SEQ(MAXCHR),FILNAM*(*),HELPF*(*) + PARAMETER (MAXPRM = 30) + CHARACTER PROMPT(2)*(MAXPRM) + IOK = 1 + IB = 1 + PROMPT(1) = 'Read file of aligned sequences' + PROMPT(2) = 'Use file of file names' + CALL RADION('Select input mode',PROMPT,2,IB, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IB.LT.1) RETURN + IF(IB.EQ.2)THEN + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,0,IOK,KBIN,KBOUT, + + 'File of sequence names', + + HELPS,HELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL RSEQ1(SEQ,MAXCHR,MAXSEQ,LENSEQ, + + IDEV2,IDEV1,KBIN,KBOUT,FILNAM,NFILE,LMAX,IOK) + RETURN + END IF + IF(IB.EQ.1) THEN + FILNAM = ' ' + CALL OPENF1(IDEV2,FILNAM,0,IOK,KBIN,KBOUT, + + 'File of aligned sequences', + + IHELPS,HELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL RSEQ2(SEQ,MAXCHR,MAXSEQ,LENSEQ, + + IDEV2,KBIN,KBOUT,FILNAM,NFILE,LMAX,IOK) + END IF + END + SUBROUTINE RSEQ1(SEQ,MAXCHR,MAXSEQ,LENSEQ, + +IDEVN,IDEVSQ,KBIN,KBOUT,FILNAM,NFILE,LMAX,IOK) + INTEGER LENSEQ(MAXSEQ) + CHARACTER SEQ(MAXCHR),FILNAM*(*) +C CONCATENATE SEQUENCES IN SEQ, PUT THE START OF SEQ(J) IN LENSEQ(J) +C SO THE LENGTH OF SEQ(J) IS LENSEQ(J+1) - LENSEQ(J) + LMAX = 0 + NFILE = 1 + LENSEQ(1) = 1 + ISEQ = 1 + REWIND IDEVN +10 CONTINUE + IF(NFILE.LT.MAXSEQ)THEN + READ(IDEVN,1000,END=20,ERR=30)FILNAM +1000 FORMAT(A) + CALL OPENRS(IDEVSQ,FILNAM,IOK,LRECL,2) + IF(IOK.NE.0)THEN + WRITE(KBOUT,1002)FILNAM +1002 FORMAT(' Error opening file ',A) + GO TO 10 + END IF + WRITE(KBOUT,1001)FILNAM +1001 FORMAT(' Reading file ',A) + IDIM = MAXCHR - ISEQ + 1 + CALL ARRFIL(IDEVSQ,SEQ(ISEQ),IDIM,KBOUT) + CLOSE(UNIT=IDEVSQ) + NFILE = NFILE + 1 + ISEQ = ISEQ + IDIM + LENSEQ(NFILE) = ISEQ + LMAX = MAX(LMAX,IDIM) + GO TO 10 + END IF +20 CONTINUE + CLOSE(UNIT=IDEVN) + IOK = 0 + WRITE(KBOUT,*)'Number of files ',NFILE - 1 + RETURN +30 CONTINUE + WRITE(KBOUT,*)' Error reading file of file names' + IOK = 1 + END + SUBROUTINE RSEQ2(SEQ,MAXCHR,MAXSEQ,LENSEQ, + +IDEVSQ,KBIN,KBOUT,FILNAM,NFILE,LMAX,IOK) + INTEGER LENSEQ(MAXSEQ) + CHARACTER SEQ(MAXCHR),LINE*80 + INTEGER NOTIRL + EXTERNAL NOTIRL + LMAX = 0 + NFILE = 0 + ISEQ = 1 +10 CONTINUE + IF(NFILE.LT.MAXSEQ)THEN + NFILE = NFILE + 1 + LENSEQ(NFILE) = ISEQ + READ(IDEVSQ,1000,ERR=40,END=30)LINE +1000 FORMAT(1X,A) + IDIM = NOTIRL(LINE,80,' ') + LMAX = MAX(LMAX,IDIM) + K = MAXCHR - ISEQ + 1 + IDIM = MIN(IDIM,K) + K = ISEQ + DO 20 I = 1,IDIM + SEQ(K) = LINE(I:I) + K = K + 1 +20 CONTINUE + ISEQ = K + GO TO 10 + END IF +30 CONTINUE + CLOSE(UNIT=IDEVSQ) + IOK = 0 + WRITE(KBOUT,*)'Number of files ',NFILE - 1 + RETURN +40 CONTINUE + WRITE(KBOUT,*)' Error reading file' + IOK = 1 + CLOSE(UNIT=IDEVSQ) + END + SUBROUTINE PLOTH(SEQ,MAXCHR,LENSEQ,NFILE,HIS,LMAX,KBIN,KBOUT, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,I1,I2, + +IHELPS,IHELPE,HELPF,IDEVH) + PARAMETER (MAXSTR = 11) + CHARACTER SEQ(MAXCHR),STRING(MAXSTR),HELPF*(*) + INTEGER LENSEQ(NFILE),HIS(LMAX) +30 CONTINUE + IDIM2 = 0 + CALL GETSTR('Word to search for',SEQ,STRING,MAXSTR,IDIM2, + +KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 30 + END IF + IF(INFLAG.NE.0) RETURN + MN = 1 + MX = IDIM2 + MINMAT = IDIM2 + CALL GETINT(MN,MX,MINMAT,'Minimum match', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINMAT = IVAL + CALL YESONO(MOPT,'Join dots','Separate dots', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(MOPT.LT.0)RETURN + XMAX=LMAX + XMIN=1 + YMIN=0 + CALL FILLI(HIS,LMAX,0) + DO 40 I = 1,NFILE-1 + K = LENSEQ(I) + I4 = LENSEQ(I+1) - K + I3 = MIN(I2,I4) + CALL SQFIT7(SEQ(K),I4,STRING,IDIM2,HIS,LMAX, + + I1,I3,MINMAT,1) +40 CONTINUE + MAXHIS = 0 + DO 50 I = 1,LMAX + IF(HIS(I).GT.MAXHIS)MAXHIS = HIS(I) +50 CONTINUE + YMAX = MAXHIS + WRITE(KBOUT,*)' Maximum in histogram=',MAXHIS + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XF=XMIN + YF=YMIN + DO 100 I = 1,LMAX + XT = I + YT = HIS(I) + IF(MOPT.EQ.0)CALL LINE(XF,XT,YF,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + IF(MOPT.EQ.1)CALL POINT(XT,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XF=XT + YF=YT +100 CONTINUE + CALL VT100M + END +C SQFIT7 + SUBROUTINE SQFIT7(SEQ,IDIM1,STRING,IDIM2,ITOTEL,ITOTID, + +IS,IE,MINS,KSTART) +C AUTHOR: RODGER STADEN +C COMPARE POINTERS, NOT ACTUAL CHARACTERS + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER ITOTEL(ITOTID) + INTEGER CTONUM + EXTERNAL CTONUM + IDIF=(IE-IS+2)-IDIM2 + IPSEQ=IS-KSTART+1 + DO 200 I=1,IDIF + NTOT = 0 + IP=IPSEQ + DO 100 J=1,IDIM2 + IF(STRING(J).EQ.'-')THEN + NTOT = NTOT + 1 + ELSE + K = CTONUM(STRING(J)) + IF(K.EQ.CTONUM(SEQ(IP)))NTOT = NTOT + 1 + END IF + IP=IP+1 +100 CONTINUE + IF(NTOT.GE.MINS)THEN + K = IP - IDIM2+KSTART-1 + ITOTEL(K) = ITOTEL(K) + 1 + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + END + SUBROUTINE PLOTP(SEQ,MAXCHR,LENSEQ,NFILE,HIS,LMAX,KBIN,KBOUT, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,I1,I2,IDEV, + +IHELPS,IHELPE,HELPF,IDEVH) + PARAMETER (MAXSTR=40) + CHARACTER SEQ(MAXCHR),STRING(MAXSTR),HELPF*(*) + INTEGER LENSEQ(NFILE),HIS(LMAX) +30 CONTINUE + IDIM2 = 0 + CALL GETSTR('Word to search for',SEQ,STRING,MAXSTR,IDIM2, + +KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 30 + END IF + IF(INFLAG.NE.0) RETURN + MN = 1 + MX = IDIM2 + MINMAT = IDIM2 + CALL GETINT(MN,MX,MINMAT,'Minimum match', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINMAT = IVAL + IGON = 0 + CALL YESONO(IGON,'Plot results','List results', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IGON.LT.0)RETURN +C CHECK RANGE + XMAX=LMAX + XMIN=1 + YMIN=0 + YMAX = 1 + IF(IGON.EQ.0)THEN + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + XMARG = REAL(MARGT)/(NFILE-1) + MARGT1 = NINT(XMARG) + MARGB1 = MARGB + END IF + NMATT = 0 + DO 200 I = 1,NFILE-1 + CALL FILLI(HIS,LMAX,0) + K = LENSEQ(I) + I4 = LENSEQ(I+1) - K + I3 = MIN(I2,I4) + CALL SQFIT8(SEQ(K),I4,STRING,IDIM2,HIS,LMAX, + + I1,I3,MINMAT,NMAT,1) + NMATT = NMATT + NMAT + YF=YMIN + YT = YMAX + DO 100 J = 1,NMAT + XT = HIS(J) + XF = XT + IF(IGON.EQ.0)THEN + CALL LINE(XF,XT,YF,YT,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB1,MARGT1,ISXMAX,ISYMAX) + END IF + IF(IGON.EQ.1)THEN + K1 = LENSEQ(I) + HIS(J) - 1 + K2 = K1 + IDIM2 - 1 + WRITE(IDEV,2000)I,HIS(J),(SEQ(K),K=K1,K2) +2000 FORMAT(' ',I6,I6,' ',20A1) + END IF +100 CONTINUE + MARGB1 = MARGB1 + MARGT1 +200 CONTINUE + CALL VT100M + END +C SQFIT8 + SUBROUTINE SQFIT8(SEQ,IDIM1,STRING,IDIM2,ITOTEL,ITOTID, + +IS,IE,MINS,ITOTP,KSTART) +C AUTHOR: RODGER STADEN +C COMPARE POINTERS, NOT ACTUAL CHARACTERS + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER ITOTEL(ITOTID) + INTEGER CTONUM + EXTERNAL CTONUM + IDIF=(IE-IS+2)-IDIM2 + IPSEQ=IS-KSTART+1 + ITOTP=0 + DO 200 I=1,IDIF + NTOT = 0 + IP=IPSEQ + DO 100 J=1,IDIM2 + IF(STRING(J).EQ.'-')THEN + NTOT = NTOT + 1 + ELSE + K = CTONUM(STRING(J)) + IF(K.EQ.CTONUM(SEQ(IP)))NTOT = NTOT + 1 + END IF + IP=IP+1 +100 CONTINUE + IF(NTOT.GE.MINS)THEN + ITOTP=ITOTP+1 + IF(ITOTP.GT.ITOTID)RETURN +C ITOT(ITOTP)=NTOT + ITOTEL(ITOTP)=IP-IDIM2+KSTART-1 + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + END + SUBROUTINE HAIRPN(SEQ,MAXCHR,LENSEQ,NFILE,LMAX, + + ITOT,ITOTEL,ITOTAL,IDTOT, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IDEV,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + INTEGER SCORES(30) + CHARACTER SEQ(MAXCHR) + INTEGER LENSEQ(NFILE) + INTEGER ITOT(IDTOT),ITOTEL(IDTOT),ITOTAL(IDTOT) + DO 1 I=1,30 + SCORES(I)=0 +1 CONTINUE + SCORES(16)=1 + SCORES(21)=1 + SCORES(22)=1 + SCORES(8)=1 + SCORES(9)=1 + SCORES(14)=1 + LEVEL=2 + IS = 1 + IN = LMAX + MINLPI = 0 + MAXLPI = 10 + MINLP = 3 + MINLPX = 1 + MAXLPX = 20 + MAXLP = 6 + MINBP = 1 + MAXBP = 20 + MININ = 6 + CALL HAIRPD(IS,IN,KBIN,KBOUT, + +MINLPI,MAXLPI,MINLP,MINLPX,MAXLPX,MAXLP,MINBP,MAXBP,MININ, + +IGON,IOK, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + XMARG = REAL(MARGT)/(NFILE-1) + MARGT1 = NINT(XMARG) + MARGB1 = MARGB + WRITE(KBOUT,*)' Searching' + IF(IGON.EQ.0) THEN + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + END IF + DO 900 II = 1,NFILE-1 + CALL FILLI(ITOT,IDTOT,0) + CALL FILLI(ITOTEL,IDTOT,0) + CALL FILLI(ITOTAL,IDTOT,0) + K = LENSEQ(II) + IDIM = LENSEQ(II+1) - LENSEQ(II) + CALL HARPN(SEQ(K),IDIM,ITOT,ITOTEL,ITOTAL,IDTOT, + + 1,IDIM,KBOUT,IP,NOUT,MINLP,MAXLP,MININ,SCORES) + IF(IP.GT.0)THEN + XMAX=LMAX + XMIN=1 +C PLOT ON SCALE 0 TO 4*MININ IN Y + YMIN=0. + YMAX=MININ*4 + YF=0. + DO 800 I=NOUT+1,IP + IF(IGON.EQ.0)CALL VECTOM + XF=ITOTEL(I)+ITOTAL(I)/2 + XT=XF + YT=ITOT(I) + IF(IGON.EQ.0)CALL LINE(XF,XT,YF,YT, + + XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB1,MARGT1,ISXMAX,ISYMAX) + IF(IGON.EQ.1)THEN + WRITE(IDEV,1000)II,ITOTEL(I)-ITOT(I)+1 +1000 FORMAT(/,' Sequence',I6,I6) + CALL DRAWPN( + + SEQ(K),IDIM,ITOT(I),ITOTAL(I),ITOTEL(I), + + IDEV,IDIM) + END IF +800 CONTINUE + END IF + MARGB1 = MARGB1 + MARGT1 +900 CONTINUE + CALL VT100M + END + SUBROUTINE HARPN(SEQ,IDIM,ITOT,ITOTEL,ITOTAL,IDTOT, + +IS,IN,KBOUT,IP,NOUT,MIN,MAX,MININ,SCORES) +C AUTHOR: RODGER STADEN + INTEGER SCORES(30) + CHARACTER SEQ(IDIM) + INTEGER ITOT(IDTOT),ITOTEL(IDTOT),ITOTAL(IDTOT) + INTEGER CTONUM + EXTERNAL CTONUM,LWRAP2 + IDO=IN-IS+1 + IF(IN.LE.IS)IDO=IDIM+IDO + I=IS-1 + IP=0 + DO 301 K=1,IDO + I=I+1 + DO 300 J=MIN,MAX + N=0 +100 CONTINUE + IMN=I-N + IPNPJ=I+N+J + IT=CTONUM(SEQ(LWRAP2(IN,IMN)))+ + + 5*CTONUM(SEQ(LWRAP2(IN,IPNPJ))) + IT=SCORES(IT) + IF(IT.NE.0)THEN + N=N+IT + GO TO 100 + END IF + IF(N.GE.MININ)THEN + IP=IP+1 + IF(IP.GT.IDTOT)THEN + WRITE(KBOUT,1234)IP,I +1234 FORMAT(' Maximum of',I5, + + ' loops found up to',I6,', no more searching') + GO TO 302 + END IF + ITOT(IP)=N + ITOTEL(IP)=LWRAP2(IN,I) + ITOTAL(IP)=J-1 + END IF +300 CONTINUE +301 CONTINUE +302 CONTINUE + IF(IP.GT.0)THEN + NOUT=0 + DO 700 J=1,IP + JP1=J+1 + D1=FLOAT(ITOTEL(J))+FLOAT(ITOTAL(J))/2. + DO 690 I=JP1,IP + D2=FLOAT(ITOTEL(I))+FLOAT(ITOTAL(I))/2. + IF(D1.NE.D2)GO TO 690 + LP2=ITOTAL(I)+2*ITOT(I) + IF(ITOTAL(J).GT.LP2)GO TO 690 + ITOT(J)=0 + ITOTAL(J)=0 + ITOTEL(J)=0 + NOUT=NOUT+1 + GO TO 700 +690 CONTINUE +700 CONTINUE + CALL BUB3AS(ITOTEL,ITOT,ITOTAL,IP) + END IF + END +C +C subroutine to draw hairpin loops on device idev +C hairpins are defined by: +C 1) nstem the number of basepairs in the stem +C 2) nloop the number of bases in the loop +C 3) ip the position of the rightmost base in the left side of the stem +C +C draw the stems very simply, just standing up with a few bases +C before and after +C maximum size is 20 up and 14 across + SUBROUTINE DRAWPN(SEQ,IDIM,NSTEM,NLOOP,IP,IDEV,IN) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),ARRAY(14,20),SCORE(5,5),SPACE + INTEGER CTONUM + EXTERNAL CTONUM + DATA SPACE/' '/ +C CHECK FOR WRAP AROUND + IF((IP-NSTEM).LT.0)RETURN + IF((IP+NLOOP+NSTEM+1).GT.IN)RETURN + DO 1 I=1,5 + DO 1 J=1,5 + SCORE(I,J)=' ' +1 CONTINUE + SCORE(1,3)='-' + SCORE(3,1)='-' + SCORE(2,4)='-' + SCORE(4,2)='-' + SCORE(1,4)='.' + SCORE(4,1)='.' + DO 2 I=1,14 + DO 2 J=1,20 + ARRAY(I,J)=SPACE +2 CONTINUE +C point to left stem start + IPL=IP-NSTEM +C point to right stem start + IPR=IP+NSTEM+NLOOP+1 +C point to temporary array + IAL=6 + IAR=8 + KA=1 +C fill in stem and loop + DO 10 I=1,MIN(NSTEM+NLOOP/2,19) + IPL=IPL+1 + IPR=IPR-1 + KA=KA+1 + ARRAY(IAL,KA)=SEQ(IPL) + ARRAY(IAR,KA)=SEQ(IPR) +C basepaired? + ARRAY(IAL+1,KA)= + + SCORE(CTONUM(SEQ(IPL)),CTONUM(SEQ(IPR))) +10 CONTINUE +C odd number of bases in loop? + IF(MOD(NLOOP,2).NE.0)THEN +C put in extra base + IF((KA+1).LE.20)ARRAY((IAL+1),KA+1)=SEQ(IPL+1) + END IF +C now do 6 bases before the stem and 7 after + IPL=IP-NSTEM-6 + DO 30 I=1,6 + IPL=IPL+1 +30 IF(IPL.GT.0)ARRAY(I,1)=SEQ(IPL) + IPR=IP+NSTEM+NLOOP + DO 40 I=8,14 + IPR=IPR+1 + IF(IPR.LT.IN)ARRAY(I,1)=SEQ(IPR) +40 CONTINUE + DO 60 I=1,20 + K=21-I + DO 50 JEMPT = 1,14 + IF(ARRAY(JEMPT,K).NE.' ') THEN + WRITE(IDEV,1000)(ARRAY(L,K),L=1,14) + GO TO 51 + END IF +50 CONTINUE +51 CONTINUE +60 CONTINUE +1000 FORMAT(' ',5X,14A1) +1001 FORMAT(' ',5X,I6) + WRITE(IDEV,1001)IP-NSTEM + END + SUBROUTINE SHOWST(NFUZ,I1,I2,KCOMP,ICOMP,IDICW,IDICS, + +IDICM,IDICH,MASK,LMASK,KBOUT) + LOGICAL MASK(LMASK) + CHARACTER STRING*25 + STRING = ' ' + DO 10 I=1,LMASK + IF (MASK(I)) THEN + STRING(I:I) = 'x' + ELSE + STRING(I:I) = '-' + END IF + 10 CONTINUE + WRITE(KBOUT,1001)STRING(1:LMASK),NFUZ,I1,I2 +1001 FORMAT(' Current mask = ',A,/, + + ' Number of mismatches =',I4,/, + + ' Start position =',I6,/, + + ' End position =',I6) + IF(KCOMP.EQ.0)WRITE(KBOUT,*)' Input strand only' + IF(KCOMP.EQ.1)WRITE(KBOUT,*)' Complementary strand only' + IF(KCOMP.EQ.2)WRITE(KBOUT,*)' Both strands' + IF(ICOMP.EQ.0)WRITE(KBOUT,*)' Even composition' + IF(ICOMP.EQ.1)WRITE(KBOUT,*)' Observed composition' + IF(ICOMP.EQ.2)WRITE(KBOUT,*)' Entered composition' + IF(IDICW.EQ.0)WRITE(KBOUT,*)' Dictionary Dw made' + IF(IDICW.NE.0)WRITE(KBOUT,*)' Dictionary Dw unmade' + IF(IDICS.NE.0)WRITE(KBOUT,*)' Dictionary Ds unmade' + IF(IDICS.EQ.0)WRITE(KBOUT,*)' Dictionary Ds made' + IF(IDICM.EQ.0)WRITE(KBOUT,*)' Dictionary Dm unmade' + IF(IDICM.EQ.1)WRITE(KBOUT,*)' Dictionary Dm made from Dw' + IF(IDICM.EQ.2)WRITE(KBOUT,*)' Dictionary Dm made from Ds' + IF(IDICH.EQ.0)WRITE(KBOUT,*)' Dictionary Dh unmade' + IF(IDICH.EQ.1)WRITE(KBOUT,*)' Dictionary Dh made from Dw' + IF(IDICH.EQ.2)WRITE(KBOUT,*)' Dictionary Dh made from Ds' + END + SUBROUTINE GETCM(COMP,KBIN,KBOUT,SEQN,LENSEQ,ICOMP, + +IHELPS,IHELPE,HELPF,IDEVH) + INTEGER SEQN(LENSEQ) + REAL COMP(5),COMPT(4) + CHARACTER BASE(4),HELPF*(*),STRING*22 + PARAMETER (MAXPRM = 20) + CHARACTER PROMPT(3)*(MAXPRM) + SAVE BASE + DATA BASE/'T','C','A','G'/ + NOPT = ICOMP + 1 + PROMPT(1) = 'Even composition' + PROMPT(2) = 'Observed composition' + PROMPT(3) = 'Type in composition' + CALL RADION('Select composition',PROMPT,3,NOPT, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(NOPT.LT.1) RETURN + ICOMP = NOPT - 1 + IF(NOPT.EQ.1) THEN + CALL FILLR(COMP,5,0.25) + RETURN + ELSE IF (NOPT.EQ.2) THEN + CALL GETCOM(SEQN,LENSEQ,COMP) + RETURN + ELSE IF (NOPT.EQ.3) THEN + RMN = 0. + TOT = 0. + DO 30 I = 1,4 + RMX = 1.0 - TOT + RMM = MIN(0.25,RMX) + WRITE(STRING,1002)BASE(I) +1002 FORMAT('Composition for base ',A1) + CALL GETRL(RMN,RMX,RMM,STRING,VAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + TOT = TOT + VAL + COMPT(I) = VAL +30 CONTINUE + CALL COPYR(COMPT,COMP,4) + RETURN + END IF + END + SUBROUTINE EXAMDW(DICTOT,WINDEX, + +MAXPOS,LENGTH,KBIN,KBOUT,WT,NFUZ,IOK,COMP,NFILE,IDEV, + +TEMPC,TEMPI,TEMPP,TEMPN,MAXLIS,MASK,LMASK) + INTEGER DICTOT(MAXPOS),WINDEX(MAXPOS),WT(4,LENGTH) + CHARACTER DCODE*8,STRING*8,STRNIN*8,UNMASK*25,SOUT*25,ST*8 + CHARACTER MMASK*25 + REAL COMP(5) + INTEGER TEMPC(MAXLIS),TEMPP(MAXLIS),TEMPI(MAXLIS),TEMPN(MAXLIS) + LOGICAL MASK(LMASK) + EXTERNAL DCODE,NCODES,STRNIN,RINF,UNMASK,MMASK + IOK = 1 + IFILL = 4 * LENGTH +30 CONTINUE + L = 0 + CALL GTSTR('Word to examine',' ',ST,L,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 30 + END IF + IF(INFLAG.NE.0) RETURN + STRING = MMASK(ST,MASK,LMASK,L) + IF(L.NE.LENGTH) RETURN + CALL FILLI(WT,IFILL,1) + CALL WFUZ2(STRING,LENGTH,NFUZ,WINDEX,MAXPOS,WT) + I = NCODES(STRING,LENGTH) + R = RINF(WT,LENGTH,COMP) + ST = STRNIN(STRING,LENGTH) + SOUT = UNMASK(ST,MASK,LMASK) + WRITE(IDEV,*)SOUT(1:LMASK),' ',DICTOT(I),R + CALL WRTWMT(WT,WT,4,LENGTH,IDEV,1) + GO TO 30 + END + SUBROUTINE EXAMDM(DICTOT,WINDEX, + +MAXPOS,LENGTH,KBIN,KBOUT,WT,NFUZ,IOK,COMP,NFILE,IDEV, + +TEMPC,TEMPI,TEMPP,TEMPN,MAXLIS,MASK,LMASK) + INTEGER DICTOT(MAXPOS),WINDEX(MAXPOS),WT(4,LENGTH) + CHARACTER DCODE*8,STRING*8,STRNIN*8 + REAL COMP(5) + LOGICAL MASK(LMASK) + INTEGER TEMPC(MAXLIS),TEMPP(MAXLIS),TEMPI(MAXLIS),TEMPN(MAXLIS) + PARAMETER (MAXPRM = 19) + CHARACTER PROMPT(2)*(MAXPRM) + EXTERNAL DCODE,NCODES,STRNIN,RINF + MAXTOT = 0 + IOK = 1 + WRITE(KBOUT,*)'Looking for highest scoring words' + IFILL = 4 * LENGTH + DO 10 I = 1,MAXPOS + IF(DICTOT(I).GT.MAXTOT)MAXTOT = DICTOT(I) +10 CONTINUE +15 CONTINUE + WRITE(KBOUT,*) + +'The highest word score = ',MAXTOT + MN = 0 + MX = MAXTOT + I = MIN(NFILE/2,MAXTOT) + CALL GETINT(MN,MX,I,'Minimum word score', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINTOT = IVAL + RI = 0. + RMN = 0. + RMX = 1.0 + CALL GETRL(RMN,RMX,RI,'Minimum information', + + VAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + XINF = VAL + I = 1 + PROMPT(1) = 'Sort on information' + PROMPT(2) = 'Sort word scores' + CALL RADION('Select sort mode',PROMPT,2,I, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(I.LT.1) RETURN + ISORT = I - 1 + MN = 0 + MX = MAXLIS + I = MAXLIS + CALL GETINT(MN,MX,I,'Maximum number to list', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXL = IVAL + IF((MINTOT.GT.0).OR.(XINF.GT.0.0))THEN + IF(MAXTOT.GE.MINTOT)THEN + WRITE(KBOUT,*)'The words are' + N = 0 + XINFM = 0. + DO 20 I = 1,MAXPOS + IF(DICTOT(I).GE.MINTOT)THEN + STRING = DCODE(I,LENGTH) + CALL FILLI(WT,IFILL,1) + CALL WFUZ2(STRING,LENGTH,NFUZ,WINDEX,MAXPOS,WT) + R = RINF(WT,LENGTH,COMP) + IF(R.GE.XINF)THEN + XINFM = MAX(XINFM,R) +C WRITE(IDEV,*)STRNIN(STRING,LENGTH),DICTOT(I),R + N = N + 1 + IF(N.LE.MAXL) THEN + TEMPI(N) = R * 10000. + TEMPC(N) = DICTOT(I) + TEMPP(N) = I + END IF + END IF + END IF +20 CONTINUE + WRITE(KBOUT,*)' Total words=',N,' Maximum information=',XINFM + MLIS = MIN(MAXL,N) + INCDEC = -1 + IF(ISORT.EQ.0) THEN + CALL SHLSRT(TEMPI,MLIS,TEMPN,INCDEC) + CALL OUTP(TEMPI,TEMPC,TEMPP,TEMPN,MLIS,LENGTH,IDEV,MASK, + + LMASK) + ELSE IF(ISORT.EQ.1) THEN + CALL SHLSRT(TEMPC,MLIS,TEMPN,INCDEC) + CALL OUTP(TEMPI,TEMPC,TEMPP,TEMPN,MLIS,LENGTH,IDEV,MASK, + + LMASK) + END IF + GO TO 15 + END IF + END IF + END + CHARACTER*(*) FUNCTION STRNIN(STRING,LENGTH) + CHARACTER STRING*(*),TUPPER + EXTERNAL TUPPER,KTONUM + CALL CCASE(STRING,2) + STRNIN(1:LENGTH) = STRING(1:LENGTH) + LO2 = LENGTH / 2 + J = LENGTH + DO 10 I = 1,LO2 + K = KTONUM(STRING(I:I)) + KTONUM(STRING(J:J)) + IF (K.EQ.5) THEN + STRNIN(I:I) = TUPPER(STRING(I:I)) + STRNIN(J:J) = TUPPER(STRING(J:J)) + END IF + J = J - 1 +10 CONTINUE + END + INTEGER FUNCTION KTONUM(CHAR) + CHARACTER CHAR + INTEGER CTONUM,MAP(5) + EXTERNAL CTONUM + SAVE MAP + DATA MAP/1,2,4,3,5/ + KTONUM = MAP(CTONUM(CHAR)) + END + SUBROUTINE MAKEDW(SEQ,MAXCHR,WINDEX,TMPDIC,MAXPOS, + +LENSEQ,MAXSEQ,NFILE,LENGTH,KCOMP,I1,I2,MASK,LMASK) + INTEGER SEQ(MAXCHR),WINDEX(MAXPOS),TMPDIC(MAXPOS) + INTEGER LENSEQ(MAXSEQ),CCODEM + LOGICAL MASK(LMASK) + EXTERNAL NCODEM,CCODEM + CALL FILLI(WINDEX,MAXPOS,0) + IF((KCOMP.EQ.0).OR.(KCOMP.EQ.2))THEN + DO 10 I = 1,NFILE-1 + I11 = LENSEQ(I) + I1 - 1 + I22 = MIN(LENSEQ(I) + I2 - 1, LENSEQ(I+1)) - LMASK + DO 5 J = I11,I22 + N = NCODEM(SEQ(J),LENGTH,MASK,LMASK) + IF(N.NE.0) WINDEX(N) = WINDEX(N) + 1 +5 CONTINUE +10 CONTINUE + END IF + IF((KCOMP.EQ.1).OR.(KCOMP.EQ.2)) THEN + DO 20 I = 1,NFILE-1 + I11 = LENSEQ(I) + I1 - 1 + I22 = MIN(LENSEQ(I) + I2 - 1, LENSEQ(I+1)) - LMASK + DO 15 J = I11,I22 + N = CCODEM(SEQ(J),LENGTH,MASK,LMASK) + IF(N.NE.0) WINDEX(N) = WINDEX(N) + 1 +15 CONTINUE +20 CONTINUE + END IF + END + INTEGER FUNCTION CCODE(STRING,LENGTH) + IMPLICIT INTEGER (A-Z) + DIMENSION CONSTS(8),STARTS(8),STRING(LENGTH),COMP(5) + SAVE CONSTS,STARTS,COMP + DATA CONSTS/1,4,16,64,256,1024,4096,16384/ + DATA STARTS/0,-4,-20,-84,-340,-1364,-5460,-21844/ + DATA COMP/3,4,1,2,5/ + CCODE = 0 + N = STARTS(LENGTH) + L = LENGTH + DO 10 I = LENGTH,1,-1 + J = STRING(I) + IF(J.NE.5) THEN + N = N + COMP(J) * CONSTS(L) + L = L - 1 + ELSE + RETURN + END IF +10 CONTINUE + CCODE = N + END + INTEGER FUNCTION CCODEM(STRING,LENGTH,MASK,LMASK) + IMPLICIT INTEGER (A-Z) + DIMENSION CONSTS(8),STARTS(8),STRING(LENGTH),COMP(5) + LOGICAL MASK(LMASK) + SAVE CONSTS,STARTS,COMP + DATA CONSTS/1,4,16,64,256,1024,4096,16384/ + DATA STARTS/0,-4,-20,-84,-340,-1364,-5460,-21844/ + DATA COMP/3,4,1,2,5/ + CCODEM = 0 + N = STARTS(LENGTH) + L = LENGTH + DO 10 I = LENGTH,1,-1 + IF (MASK(I)) THEN + J = STRING(I) + IF(J.NE.5) THEN + N = N + COMP(J) * CONSTS(L) + L = L - 1 + ELSE + RETURN + END IF + END IF +10 CONTINUE + CCODEM = N + END + INTEGER FUNCTION NCODEM(STRING,LENGTH,MASK,LMASK) + IMPLICIT INTEGER (A-Z) + DIMENSION CONSTS(8),STARTS(8),STRING(LENGTH) + LOGICAL MASK(LMASK) + SAVE CONSTS,STARTS + DATA CONSTS/1,4,16,64,256,1024,4096,16384/ + DATA STARTS/0,-4,-20,-84,-340,-1364,-5460,-21844/ + NCODEM = 0 + N = STARTS(LENGTH) + L = LENGTH + DO 10 I = 1,LMASK + IF (MASK(I)) THEN + J = STRING(I) + IF(J.NE.5) THEN + N = N + J * CONSTS(L) + L = L - 1 + ELSE + RETURN + END IF + END IF +10 CONTINUE + NCODEM = N + END + INTEGER FUNCTION NCODE(STRING,LENGTH) + IMPLICIT INTEGER (A-Z) + DIMENSION CONSTS(8),STARTS(8),STRING(LENGTH) + SAVE CONSTS,STARTS + DATA CONSTS/1,4,16,64,256,1024,4096,16384/ + DATA STARTS/0,-4,-20,-84,-340,-1364,-5460,-21844/ + NCODE = 0 + N = STARTS(LENGTH) + L = LENGTH + DO 10 I = 1,LENGTH + J = STRING(I) + IF(J.NE.5) THEN + N = N + J * CONSTS(L) + L = L - 1 + ELSE + RETURN + END IF +10 CONTINUE + NCODE = N + END + INTEGER FUNCTION NCODES(STRING,LENGTH) + INTEGER CONSTS(8),STARTS(8) + CHARACTER STRING*(*) + INTEGER CTONUM + EXTERNAL CTONUM + SAVE CONSTS,STARTS + DATA CONSTS/1,4,16,64,256,1024,4096,16384/ + DATA STARTS/0,-4,-20,-84,-340,-1364,-5460,-21844/ + NCODES = STARTS(LENGTH) + DO 10 I = 1,LENGTH + NCODES = NCODES + CTONUM(STRING(I:I)) * CONSTS(LENGTH-I+1) +10 CONTINUE + END + SUBROUTINE WFUZ2(WORD,LENGTH,NFUZ,WINDEX,MAXPOS,WT) + INTEGER STRING(8),WINDEX(MAXPOS) + INTEGER DIF(3,4),WT(4,LENGTH) + INTEGER CTONUM + CHARACTER WORD*(*),CHAR + EXTERNAL CTONUM,NCODE + SAVE DIF + DATA DIF/2,3,4, + + 1,3,4, + + 1,2,4, + + 1,2,3/ + DO 1 I = 1,LENGTH + CHAR = WORD(I:I) + STRING(I) = CTONUM(CHAR) +1 CONTINUE +C KEEP ADDING COUNTS UNTIL LEVEL OF FUZ EXCEEDED + NALL = NCODE(STRING(1),LENGTH) + IF(WINDEX(NALL).NE.0) + + CALL WDCODE(WT,STRING,LENGTH,WINDEX(NALL)) + IF(NFUZ.EQ.0)RETURN +C ONLY ONE POSITION MUST CHANGE, CHANGE THEM ALL IN TURN + N = 1 + DO 10 I1 = 1,LENGTH + DO 5 I2 = 1,3 + N = N + 1 + IT = STRING(I1) + NC1 = DIF(I2,STRING(I1)) + STRING(I1) = NC1 + NALL = NCODE(STRING(1),LENGTH) + IF(WINDEX(NALL).NE.0) + + CALL WDCODE(WT,STRING,LENGTH,WINDEX(NALL)) + STRING(I1) = IT +5 CONTINUE +10 CONTINUE + IF(NFUZ.EQ.1)RETURN +C TWO POSITIONS MUST CHANGE, CHANGE THEM ALL IN TURN + DO 40 I1 = 1,LENGTH + DO 30 I2 = I1+1,LENGTH + DO 25 I3 = 1,3 + IT1 = STRING(I1) + NC1 = DIF(I3,STRING(I1)) + STRING(I1) = NC1 + DO 24 I4 = 1,3 + IT2 = STRING(I2) + NC2 = DIF(I4,STRING(I2)) + STRING(I2) = NC2 + N = N + 1 + NALL = NCODE(STRING(1),LENGTH) + IF(WINDEX(NALL).NE.0) + + CALL WDCODE(WT,STRING,LENGTH,WINDEX(NALL)) + STRING(I2) = IT2 +24 CONTINUE + STRING(I1) = IT1 +25 CONTINUE +30 CONTINUE +40 CONTINUE + END + SUBROUTINE MAKEDM(WINDEX,DM,LENGTH,NFUZ,MAXPOS) + INTEGER STRING(8),WINDEX(MAXPOS),DM(MAXPOS),DIF(3,4) + SAVE DIF + EXTERNAL NCODE + DATA DIF/2,3,4, + + 1,3,4, + + 1,2,4, + + 1,2,3/ +C IF(NFUZ.EQ.0)THEN + DO 100 IWORD = 1,MAXPOS +C CHANGED NEXT LINE 16-7-89 +C DICTOT(IWORD) = DICTOT(IWORD) + WINDEX(IWORD) + DM(IWORD) = WINDEX(IWORD) +100 CONTINUE + IF(NFUZ.EQ.0) RETURN + DO 200 IWORD = 1,MAXPOS +C ONLY ONE POSITION MUST CHANGE, CHANGE THEM ALL IN TURN +C IF THIS WORD EXISTS IN SEQ + KT = WINDEX(IWORD) + IF(KT.NE.0)THEN + CALL DCODEI(IWORD,LENGTH,STRING) + DO 10 I1 = 1,LENGTH + DO 5 I2 = 1,3 + IT = STRING(I1) + NC1 = DIF(I2,STRING(I1)) + STRING(I1) = NC1 + JWORD = NCODE(STRING(1),LENGTH) + DM(JWORD) = DM(JWORD) + KT + STRING(I1) = IT +5 CONTINUE +10 CONTINUE + END IF +200 CONTINUE + IF(NFUZ.EQ.1)RETURN + DO 300 IWORD = 1,MAXPOS +C TWO POSITIONS MUST CHANGE, CHANGE THEM ALL IN TURN + KT = WINDEX(IWORD) + IF(KT.NE.0)THEN + CALL DCODEI(IWORD,LENGTH,STRING) + DO 40 I1 = 1,LENGTH + DO 30 I2 = I1+1,LENGTH + DO 25 I3 = 1,3 + IT1 = STRING(I1) + NC1 = DIF(I3,STRING(I1)) + STRING(I1) = NC1 + DO 24 I4 = 1,3 + IT2 = STRING(I2) + NC2 = DIF(I4,STRING(I2)) + STRING(I2) = NC2 + JWORD = NCODE(STRING(1),LENGTH) + DM(JWORD) = DM(JWORD) + KT + STRING(I2) = IT2 +24 CONTINUE + STRING(I1) = IT1 +25 CONTINUE +30 CONTINUE +40 CONTINUE + END IF +300 CONTINUE + RETURN +C END IF + END + SUBROUTINE MAKEDH(TMPDIC,DICTOT,LENGTH,NFUZ,MAXPOS) + INTEGER STRING(8),TMPDIC(MAXPOS),DICTOT(MAXPOS) + INTEGER GETV + EXTERNAL GETV + DO 100 IWORD = 1,MAXPOS + TMPDIC(IWORD) = DICTOT(IWORD) +100 CONTINUE + IF(NFUZ.EQ.0) RETURN + DO 200 IWORD = 1,MAXPOS +C ONLY ONE POSITION MUST CHANGE, CHANGE THEM ALL IN TURN +C IF THIS WORD EXISTS IN SEQ + IF(DICTOT(IWORD).NE.0)THEN + CALL DCODEI(IWORD,LENGTH,STRING) + IV = GETV(IWORD,LENGTH,STRING,DICTOT,MAXPOS,NFUZ) + IF(IV.GT.TMPDIC(IWORD))TMPDIC(IWORD) = 0 + END IF +200 CONTINUE + IF(NFUZ.EQ.1)RETURN + DO 300 IWORD = 1,MAXPOS +C TWO POSITIONS MUST CHANGE, CHANGE THEM ALL IN TURN + IF(DICTOT(IWORD).NE.0)THEN + CALL DCODEI(IWORD,LENGTH,STRING) + IV = GETV(IWORD,LENGTH,STRING,DICTOT,MAXPOS,NFUZ) + IF(IV.GT.TMPDIC(IWORD))TMPDIC(IWORD) = 0 + END IF +300 CONTINUE + RETURN + END + INTEGER FUNCTION GETV(IWORD,LENGTH,STRING,DICTOT,MAXPOS,NFUZ) + INTEGER DICTOT(MAXPOS),STRING(8) + INTEGER DIF(3,4) + EXTERNAL NCODE + SAVE DIF + DATA DIF/2,3,4, + + 1,3,4, + + 1,2,4, + + 1,2,3/ + IV = DICTOT(IWORD) + DO 10 I1 = 1,LENGTH + DO 5 I2 = 1,3 + IT = STRING(I1) + NC1 = DIF(I2,STRING(I1)) + STRING(I1) = NC1 + JWORD = NCODE(STRING(1),LENGTH) + IV = MAX(IV,DICTOT(JWORD)) + STRING(I1) = IT +5 CONTINUE +10 CONTINUE + IF(NFUZ.EQ.1)THEN + GETV = IV + RETURN + END IF + DO 40 I1 = 1,LENGTH + DO 30 I2 = I1+1,LENGTH + DO 25 I3 = 1,3 + IT1 = STRING(I1) + NC1 = DIF(I3,STRING(I1)) + STRING(I1) = NC1 + DO 24 I4 = 1,3 + IT2 = STRING(I2) + NC2 = DIF(I4,STRING(I2)) + STRING(I2) = NC2 + JWORD = NCODE(STRING(1),LENGTH) + IV = MAX(IV,DICTOT(JWORD)) + STRING(I2) = IT2 +24 CONTINUE + STRING(I1) = IT1 +25 CONTINUE +30 CONTINUE +40 CONTINUE + GETV = IV + END + SUBROUTINE WDCODE(WT,STRING,LENGTH,IADD) + INTEGER WT(4,LENGTH),STRING(LENGTH) + DO 10 I = 1,LENGTH + WT(STRING(I),I) = WT(STRING(I),I) + IADD +10 CONTINUE + END + REAL FUNCTION RINF(WT,LENGTH,COMP) + INTEGER WT(4,LENGTH) + REAL COMP(5) + HSS = 0. + T = 0. + DO 1 I = 1,4 + T = T + WT(I,1) +1 CONTINUE + DO 10 I = 1,LENGTH + DO 5 J = 1,4 + HS = 0. + Y = COMP(J) + X = REAL(WT(J,I))/T + HS = X * LOG(X/Y) + HSS = HSS + HS +5 CONTINUE +10 CONTINUE + RINF = HSS/REAL(LENGTH) + END + SUBROUTINE DCODEI(A,LENGTH,STRING) + INTEGER A,CONSTS(8),STRING(LENGTH) + SAVE CONSTS + DATA CONSTS/1,4,16,64,256,1024,4096,16384/ + K = 0 + J = A + DO 10 I = LENGTH,1,-1 + J1 = (J-1) / CONSTS(I) + K = K + 1 + STRING(K) = J1 + 1 + J = J - J1*CONSTS(I) +10 CONTINUE + END + SUBROUTINE WRTWMT(WEIGHT,IWT,MAXCHR,LENGTH,IDEV,IFLAG) + REAL WEIGHT(MAXCHR,LENGTH) + INTEGER IWT(MAXCHR,LENGTH) + CHARACTER STRING*8 + CHARACTER WTMCON + EXTERNAL WTMCON + DO 10 I = 1,MAXCHR + IF(IFLAG.EQ.0) + + WRITE(IDEV,1000,ERR=100)(WEIGHT(I,J),J=1,LENGTH) + IF(IFLAG.EQ.1) + + WRITE(IDEV,1001,ERR=100)(IWT(I,J),J=1,LENGTH) +10 CONTINUE +1000 FORMAT(' ',20F5.2) +1001 FORMAT(' ',20I6) +100 CONTINUE + STRING = ' ' + DO 20 I = 1,LENGTH + STRING(I:I) = WTMCON(IWT,LENGTH,MAXCHR,I) +20 CONTINUE + WRITE(IDEV,1002)STRING +1002 FORMAT(' ',A) + END + CHARACTER*1 FUNCTION WTMCON(IWT,LENGTH,MAXCHR,IP) + INTEGER IWT(MAXCHR,LENGTH),C(5) + CHARACTER STRING(5,5) + SAVE STRING + DATA STRING/ + +'T','Y','W','K','T', + +'Y','C','M','S','C', + +'W','M','A','R','A', + +'K','S','R','G','G', + +'T','C','A','G','-'/ + N = 0 + DO 10 J = 1,MAXCHR + I = IWT(J,IP) + IF (I.GT.N) N = I +10 CONTINUE + NC = 0 + DO 20 J = 1,MAXCHR + C(J) = 0 + I = IWT(J,IP) + IF (I.EQ.N) THEN + M = J + NC = NC + 1 + C(NC) = J + END IF +20 CONTINUE + IF(NC.EQ.1)THEN + J = C(1) + WTMCON = STRING(J,J) + RETURN + END IF + IF(NC.EQ.2)THEN + J = C(1) + I = C(2) + WTMCON = STRING(I,J) + RETURN + END IF + IF(NC.EQ.3)THEN + J = 0 + DO 30 I = 1,3 + J = J + C(I) +30 CONTINUE + IF(J.EQ.6) WTMCON = 'H' + IF(J.EQ.7) WTMCON = 'B' + IF(J.EQ.8) WTMCON = 'D' + IF(J.EQ.9) WTMCON = 'V' + RETURN + END IF + WTMCON = 'N' + END + CHARACTER*(*) FUNCTION DCODE(A,LENGTH) + IMPLICIT INTEGER (A-Z) + DIMENSION CONSTS(8) + CHARACTER BASE(5) + SAVE CONSTS,BASE + DATA BASE/'T','C','A','G','-'/ + DATA CONSTS/1,4,16,64,256,1024,4096,16384/ + K = 0 + J = A + DCODE = ' ' + DO 10 I = LENGTH,1,-1 + J1 = (J-1) / CONSTS(I) + K = K + 1 + DCODE(K:K) = BASE(J1+1) + J = J - J1*CONSTS(I) +10 CONTINUE + END + SUBROUTINE GETCOM(SEQN,IDIM,COMP) + INTEGER SEQN(IDIM) + REAL COMP(5) +C NB ADD 1 TO EACH BASE FOR LATER + CALL FILLR(COMP,5,1.) + DO 10 I = 1,IDIM + J = SEQN(I) + COMP(J) = COMP(J) + 1. +10 CONTINUE + X = REAL(IDIM) + 4. + DO 20 I = 1,4 + COMP(I) = COMP(I)/X +20 CONTINUE + END + SUBROUTINE TCOMP(COMP,KCOMP) + REAL COMP(5) + IF(KCOMP.EQ.1)THEN + T1 = COMP(1) + COMP(1) = COMP(3) + COMP(3) = T1 + T1 = COMP(2) + COMP(2) = COMP(4) + COMP(4) = T1 + ELSE IF (KCOMP.EQ.2)THEN + T1 = COMP(1) + T2 = COMP(3) + T1 = (T1 + T2) / 2. + COMP(1) = T1 + COMP(3) = T1 + T1 = COMP(2) + T2 = COMP(4) + T1 = (T1 + T2) / 2. + COMP(2) = T1 + COMP(4) = T1 + END IF + END + SUBROUTINE WRTDIC(IDEV,FILNAM,DIC,MAXPOS,IOK) + INTEGER DIC(MAXPOS) + CHARACTER FILNAM*(*) + CALL OPENRS(IDEV,FILNAM,IOK,LRECL,6) + IF(IOK.NE.0)RETURN + WRITE(IDEV)DIC + CLOSE(UNIT=IDEV) + END + SUBROUTINE RDDIC(IDEV,FILNAM,DIC,MAXPOS,IOK) + INTEGER DIC(MAXPOS) + CHARACTER FILNAM*(*) + CALL OPENRS(IDEV,FILNAM,IOK,LRECL,7) + IF(IOK.NE.0)RETURN + READ(IDEV)DIC + CLOSE(UNIT=IDEV) + END + SUBROUTINE RDIC(IDEV,FILNAM,DIC,MAXPOS,KBIN,KBOUT,IOK, + +IHELPS,IHELPE,HELPF,IDEVH) + INTEGER DIC(MAXPOS) + CHARACTER FILNAM*(*),HELPF*(*) +30 CONTINUE + L = 0 + CALL GTSTR( + +'Name of dictionary file',' ',FILNAM,L,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 30 + END IF + IF(INFLAG.NE.0) RETURN + CALL RDDIC(IDEV,FILNAM,DIC,MAXPOS,IOK) + END + SUBROUTINE WDIC(IDEV,FILNAM,DIC,MAXPOS,KBIN,KBOUT,IOK, + +IHELPS,IHELPE,HELPF,IDEVH) + INTEGER DIC(MAXPOS) + CHARACTER FILNAM*(*),HELPF*(*) +30 CONTINUE + L = 0 + CALL GTSTR( + +'Name for dictionary file',' ',FILNAM,L,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 30 + END IF + IF(INFLAG.NE.0) RETURN + CALL WRTDIC(IDEV,FILNAM,DIC,MAXPOS,IOK) + END + SUBROUTINE DICIO(KBIN,KBOUT,NOPT,IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + PARAMETER (MAXPRM = 10) + CHARACTER PROMPT(8)*(MAXPRM) + WRITE(KBOUT,*)'Save or restore a dictionary' + NOPT = 0 + IB = 1 + PROMPT(1) = 'Save Dw' + PROMPT(2) = 'Save Ds' + PROMPT(3) = 'Save Dm' + PROMPT(4) = 'Save Dh' + PROMPT(5) = 'Restore Dw' + PROMPT(6) = 'Restore Ds' + PROMPT(7) = 'Restore Dm' + PROMPT(8) = 'Restore Dh' + CALL RADION('Select save or restore mode',PROMPT,8,IB, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IB.LT.1) RETURN + NOPT = IB + END + SUBROUTINE SHLSRT(KEY,N,IPOINT,INCDEC) +C SHELL SORT +C REFERENCES: D.L. SHELL, CACM 2, 32 (JULY 1959) +C D.E. KNUTH, TAOCP III, SECT. 5.2.1 +C CALLING SEQUENCE: +C +C KEY IS AN ARRAY OF KEYS ON WHICH TO SORT +C N IS THE NUMBER OF ITEMS +C IPOINT IS THE ARRAY OF POINTERS +C (ONLY THE POINTERS WILL MOVE) +C INCDEC .GE. 0 FOR SORTING INTO INCREASING ORDER; +C .LT. 0 FOR SORTING INTO DECREASING ORDER +C + INTEGER KEY(N),IPOINT(N),K + INTEGER H,S,T + IPOINT(1) = 1 + IF(N.LT.2)RETURN +C INITIALIZE POINTER ARRAY + DO 2 I = 2,N + IPOINT(I) = I + 2 CONTINUE +C +C CHOICE OF SEQUENCE OF INCREMENTS SUGGESTED +C BY KNUTH III, EQ. 8, P. 95. HIS FORMULA +C IS EQUIVALENT TO: +C +C H(S) = (3**S - 1)/2 +C INITIAL VALUE OF S IS MINIMAL INTEGER +C SUCH THAT H(S+2) .GE. N +C +C +C SMAX = (ALOG(2N + 1)/ALOG(3)) - 2 + 1 + S = INT( (ALOG(FLOAT(2*N+1))/1.09861229) - 0.95 ) + S = MAX(S,1) + H = (3**S - 1)/2 + DO 7 T = 1,S + JMIN = H + 1 + DO 6 J = JMIN,N + I = J - H + JJ = IPOINT(J) + K = KEY(JJ) + IPT = IPOINT(J) + 3 CONTINUE + II = IPOINT(I) + IF((K-KEY(II)).LE.0)THEN + IPLUSH = I + H + IPOINT(IPLUSH) = IPOINT(I) + I = I - H + IF(I.GT.0) GO TO 3 + END IF + IPLUSH = I + H + IPOINT(IPLUSH) = IPT + 6 CONTINUE +C CHANGE INCREMENT + IF(H.LT.2) GO TO 8 + H = (H-1)/3 +7 CONTINUE +C CHECK INCDEC: IF NEGATIVE, SWITCH POINTER ARRAY +8 CONTINUE + IF(INCDEC.LT.0)THEN + M = N/2 + NP1MI = N + DO 10 I = 1,M + NTEMP = IPOINT(I) + IPOINT(I) = IPOINT(NP1MI) + IPOINT(NP1MI) = NTEMP + NP1MI = NP1MI - 1 + 10 CONTINUE + END IF + END + SUBROUTINE MAKEDS(SEQ,MAXCHR,DS,DT,MAXPOS, + +LENSEQ,MAXSEQ,NFILE,LENGTH,KCOMP,I1,I2,TEMP,MASK,LMASK) + INTEGER SEQ(MAXCHR),DS(MAXPOS),DT(MAXPOS) + INTEGER LENSEQ(MAXSEQ),CCODEM,TEMP(MAXCHR) + LOGICAL MASK(LMASK) + EXTERNAL NCODEM,CCODEM + CALL FILLI(DS,MAXPOS,0) + CALL FILLI(DT,MAXPOS,0) + ITEMP = 0 + IF((KCOMP.EQ.0).OR.(KCOMP.EQ.2))THEN + DO 10 I = 1,NFILE-1 + I11 = LENSEQ(I) + I1 - 1 + I22 = MIN(LENSEQ(I) + I2 - 1, LENSEQ(I+1)) - LMASK + IF(ITEMP.GT.0) THEN + DO 4 J = 1,ITEMP + DT(TEMP(J)) = 0 +4 CONTINUE + ITEMP = 0 + END IF + DO 5 J = I11,I22 + N = NCODEM(SEQ(J),LENGTH,MASK,LMASK) + IF(N.NE.0)THEN + IF(DT(N).EQ.0) THEN + DT(N) = 1 + ITEMP = ITEMP + 1 + TEMP(ITEMP) = N + DS(N) = DS(N) + 1 + END IF + END IF +5 CONTINUE +10 CONTINUE + END IF + IF((KCOMP.EQ.1).OR.(KCOMP.EQ.2)) THEN + DO 20 I = 1,NFILE-1 + I11 = LENSEQ(I) + I1 - 1 + I22 = MIN(LENSEQ(I) + I2 - 1, LENSEQ(I+1)) - LMASK + IF(ITEMP.GT.0) THEN + DO 14 J = 1,ITEMP + DT(TEMP(J)) = 0 +14 CONTINUE + ITEMP = 0 + END IF + DO 15 J = I11,I22 + N = CCODEM(SEQ(J),LENGTH,MASK,LMASK) + IF(N.NE.0)THEN + IF(DT(N).EQ.0) THEN + DT(N) = 1 + ITEMP = ITEMP + 1 + TEMP(ITEMP) = N + DS(N) = DS(N) + 1 + END IF + END IF +15 CONTINUE +20 CONTINUE + END IF + END + SUBROUTINE OUTP(TEMPI,TEMPC,TEMPP,TEMPN,MLIS,LENGTH,IDEV, + +MASK,LMASK) + INTEGER TEMPI(MLIS),TEMPC(MLIS),TEMPP(MLIS),TEMPN(MLIS) + CHARACTER*8 STRING,DCODE + CHARACTER UNMASK*25,SOUT*25 + LOGICAL MASK(LMASK) + EXTERNAL DCODE,UMASK + DO 100 I = 1,MLIS + N = TEMPN(I) + NW = TEMPP(N) + STRING = DCODE(NW,LENGTH) + SOUT = UNMASK(STRING,MASK,LMASK) + WRITE(IDEV,1000)SOUT(1:LMASK),TEMPC(N),REAL(TEMPI(N))/10000. +1000 FORMAT(' ',A,' ',I6,F10.5) +100 CONTINUE + END + SUBROUTINE HAIRPD(IS,IN,KBIN,KBOUT, + +MINLPI,MAXLPI,MINLP,MINLPX,MAXLPX,MAXLP,MINBP,MAXBP,MININ, + +IGON,IOK, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN + WRITE(KBOUT,1004) +1004 FORMAT(' Define the range of loop sizes') + CALL GETINT(MINLPI,MAXLPI,MINLP,'Minimum loop size', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MINLP = IVAL + MINLPX = MAX(MINLPX,MINLP) + MAXLP = MINLPX + CALL GETINT(MINLPX,MAXLPX,MAXLP,'Maximum loop size', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXLP = IVAL + MINLP = MINLP + 1 + MAXLP = MAXLP + 1 + CALL GETINT(MINBP,MAXBP,MININ,'Minimum number of basepairs', + +IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MININ = IVAL + CALL YESONO(IGON,'Plot results','List results', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) +C IF(IGON.LT.0)RETURN + END +C lwrap2 + INTEGER FUNCTION LWRAP2(IDIM,I) +C AUTHOR: RODGER STADEN +C test for end of array,ifso wrap around + LWRAP2=I + IF(LWRAP2.LT.1)LWRAP2=LWRAP2+IDIM+1 + IF(LWRAP2.GT.IDIM)LWRAP2=LWRAP2-IDIM + END + SUBROUTINE LISTN(SEQ,MAXCHR,LENSEQ,NFILE,LMAX,LINLEN,I1,I2, + +IDEV,KBOUT) + CHARACTER SEQ(MAXCHR) + INTEGER LENSEQ(NFILE) +C SET WIDTH FOR LAST PAGE + LPAGE=MOD(I2-I1+1,LINLEN) +C HOW MANY PAGE WIDTHS? + NPAGE=1+(I2-I1+1)/LINLEN + IF(MOD(I2-I1+1,LINLEN).EQ.0)THEN + NPAGE=NPAGE-1 + LPAGE=LINLEN + END IF + ISTART=I1-LINLEN + DO 50 I=1,NPAGE + ISTART=ISTART+LINLEN + IF(I.EQ.NPAGE)LINLEN=LPAGE + WRITE(IDEV,1006)(K,K=ISTART+9,ISTART+LINLEN-1,10) + DO 40 J=1,NFILE-1 + KF = ISTART + LENSEQ(J) - 1 + KT = MIN(KF+LINLEN,LENSEQ(J+1)) - 1 + WRITE(IDEV,1003,ERR=60)J,(SEQ(K),K=KF,KT) +1003 FORMAT(' ',I4,' ',100A1) +1006 FORMAT(' ',10I10) +40 CONTINUE + WRITE(IDEV,1008) +1008 FORMAT(/) +50 CONTINUE + RETURN +60 CONTINUE + WRITE(KBOUT,*)' Error writing file' + END + SUBROUTINE GMASK(KBIN,KBOUT,MASK,LMASK1,LENGTH, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER CMASK*25,HELPF*(*),TUPPER + LOGICAL MASK(LMASK1) + EXTERNAL TUPPER + 1 CONTINUE + LMASK = LMASK1 + DO 2 I=1,LMASK + MASK(I) = .TRUE. + 2 CONTINUE + LMASK = LENGTH +5 CONTINUE + WRITE(KBOUT,1002) +1002 FORMAT(' x means use, - means ignore',/, + + ' e.g. xx-x---x-x means use positions 1,2,4,8,10') + LIN = 0 + CALL GTSTR('Mask',' ',CMASK,LIN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 5 + END IF + IF(INFLAG.EQ.2) RETURN + IF(LIN.EQ.0) RETURN + LMASK = LIN + LENGTH = 0 + DO 70 I=1,LIN + IF(TUPPER(CMASK(I:I)).EQ.'X') THEN + LENGTH = LENGTH + 1 + MASK(I) = .TRUE. + ELSE + MASK(I) = .FALSE. + END IF +70 CONTINUE + IF (LENGTH.GT.8) THEN + WRITE(KBOUT,*)'More than 8 active bases' + GO TO 1 + END IF + LMASK1 = LMASK + END + CHARACTER*(*) FUNCTION UNMASK(STRING,MASK,LMASK) + CHARACTER STRING*(*) + LOGICAL MASK(LMASK) + UNMASK = ' ' + DO 5 I=1,LMASK + UNMASK(I:I) = '-' + 5 CONTINUE + J = 0 + DO 10 I=1,LMASK + IF (MASK(I)) THEN + J = J + 1 + UNMASK(I:I) = STRING(J:J) + END IF + 10 CONTINUE + END + CHARACTER*(*) FUNCTION MMASK(STRING,MASK,LMASK,J) + CHARACTER STRING*(*) + LOGICAL MASK(LMASK) + J = 0 + DO 10 I=1,LMASK + IF (MASK(I)) THEN + J = J + 1 + MMASK(J:J) = STRING(I:I) + END IF + 10 CONTINUE + END diff --git a/src/staden/mystdlib.h b/src/staden/mystdlib.h new file mode 100644 index 0000000..7a51472 --- /dev/null +++ b/src/staden/mystdlib.h @@ -0,0 +1,38 @@ +/* + Title: stdlib + + File: mystdlib.h + Purpose: ANSII C stdlib.h replacement + Last update: Thu May 24 1990 +*/ + + +/* + The SUN does not provide the ANSII C stdlib.h header file, so + so this file provides some of the definitions which should be + found there. + + Note that we cannot override existing type definitions. + + (3/3/93) For this reason this file is now no longer needed. If you are on + a system needing this then define NOSTDLIB in the makefile. +*/ + +#ifdef NOSTDLIB + + +/* String processing functions */ +extern double strtod(char *str, char **ptr); +extern double atof(char *str); +extern long strtol(char *str, char **ptr, int base); + + +/* Control functions */ +extern void exit(int status); + + +/* Storage allocation */ +extern char *malloc(unsigned size); +extern char *calloc(unsigned elt_count, unsigned elt_size); + +#endif diff --git a/src/staden/nip.f b/src/staden/nip.f new file mode 100644 index 0000000..0d49c49 --- /dev/null +++ b/src/staden/nip.f @@ -0,0 +1,1023 @@ +C NIP Nucleotide interpretation program +C +C author: Rodger Staden, Medical Research Council Centre, +C Laboratory of Molecular Biology, Hills Road, +C Cambridge, England +C 12-1-90 Closed idev if.ne.kbout after rdseq +C 16-3-90 Removed escape after bpause +C 5-4-90 Changed getdev to redir and removed all close(unit=idev) +C etc (see redir) +C 6-7-90 Added showfi +C 9-7-90 Renamed amenu to menu +C 14-8-90 Modified 6 phase translation +C 22-8-90 Modified 6 phase translation so it will do all listings +C 24-9-90 Fixed bug in graphics output for pattern search +C 5-11-90 Very many changes for addition of file of file names +C search for patterns - affects patternn,patternnc,nipl, +C nip,anals89,asubs89 (rdwmt) +C 11-12-90 Chabges relating to new way of handling pir libs. New filnll +C here and new parameter +C 11-1-91 Modified positional base preferences to allow standard to be +C read form a file: call from main has extra arguments +C 22-2-91 For find open frames and trandk send maxseq instead of idim3 +C in odrer to give sufficient space for 6 phase translation +C 17-4-91 New splice junction search +C 7-5-91 Many changes to do with use of feature tables +C including the argument lists to: codons, codtdk, trandk, opentr, pltmap, +C codimp. Also forced out use of option 38 by caling 39 instead +C 7-6-91 New sequence library routines for cdrom format. PIR stuff junked +C 18-7-91 trndom did not write title! +C 18-7-91 Added titles to pattern files +C 25-2-92 Changed call to rdseq !!!!!!!! +C 8-5-92 upped maxseq to 330000 +C 17-6-92 Declared SIGNAL as an external as there's an intrinsic with +C the same name under AIX + SUBROUTINE FMAIN() + INTEGER BOTOPT,TOPOPT + PARAMETER (NAMLEN = 60) + CHARACTER*(NAMLEN) FILE1,FILE2,FILE3,FILE4,FILE5,FILE6,FILE7 + CHARACTER*(NAMLEN) HELPF,POINTF,FILMAR,FILNAM,FILEIN + CHARACTER*(NAMLEN) FOFNAM + CHARACTER*(NAMLEN) LIBLF + PARAMETER (BOTOPT=-10,TOPOPT=70, + + MAXSEQ=330000, + + MXSPAN=603, + + MAXWIN=MAXSEQ+MXSPAN, + + MAXWIR=330000, + + MAXD48=MAXWIR/48, + + MAXD2=MAXWIR/2, + + MAXD3=MAXWIR/3, + + MAXSD2=MAXSEQ/2, + + MAXSD3=MAXSEQ/3, + + MAXMEN=-8, + + MAXOPT=67, + + MAXDEV=9, + + MXWTLN = 120, + + IDM = 5, + + IDME = 17) + PARAMETER (FILE1='EUKRIBS', + + FILE2='INTRONS', + + FILE3='ECPROMS', + + FILE4='ECRIBS', + + FILE5='RENZYM4', + + FILE6='RENZYM6', + + FILE7='RENZYMAL', + + HELPF='NIPHELP', + + POINTF='NIPHPNT', + + FILMAR='NIPMARG', + + LIBLF = 'SEQUENCELIBRARIES') + PARAMETER (MAXMOT = 50, MAXWTS = 4000) + CHARACTER*(NAMLEN) NAMSAV(MAXMOT) + CHARACTER*8 KEYNS(MAXMOT) + INTEGER HELPS(BOTOPT:TOPOPT),HELPE(BOTOPT:TOPOPT),DEVNOS(MAXDEV) + REAL WORKR(MAXWIR) + INTEGER WORKI(MAXWIR),OPT,MARGB(MAXOPT),MARGT(MAXOPT) + PARAMETER (MAXWRD = 6) + PARAMETER (MAXDIC = 4**MAXWRD) + INTEGER WORDP(MAXDIC),WORDN(MAXDIC) + PARAMETER (MAXNZ1 = MAXSEQ/10, + + MAXEN = 1000, + + MAXEN8 = MAXEN*8) + INTEGER ENZYM1(MAXNZ1),ENZYM2(MAXNZ1),ENZYM3(MAXNZ1) + INTEGER ENZYME(MAXEN8),ENZYM4(MAXNZ1) +C MAT1 SIMPLE IDENTITY +C MAT2 IUB SCORES 0-1 +C MAT3 IUB SCORES 0-36 +C MAT4 INVERTED REPEAT + INTEGER MAT1(IDM,IDM),MAT2(IDME,IDME) + INTEGER MAT3(IDME,IDME),MAT4(IDM,IDM) + CHARACTER SEQ(MAXWIN),SEQW(MAXSEQ),PAA(5,5,5),PAAS(5,5,5) + CHARACTER CHRSET(IDM) + EQUIVALENCE (WORKR,WORKI) + EQUIVALENCE (ENZYME,WORDN) +C EXTERNALS + EXTERNAL SIGNAL + DATA CHRSET/'T','C','A','G','-'/ + DATA MAT1/ + + 1,0,0,0,0, + + 0,1,0,0,0, + + 0,0,1,0,0, + + 0,0,0,1,0, + + 0,0,0,0,0/ + DATA MAT2/ + +1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,0,1,0,0,0,0,0,0,0,0,0,0,0, + +1,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0,0, + +1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0, + +0,1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0, + +0,1,1,0,0,0,0,0,0,1,0,0,0,0,0,0,0, + +1,0,0,1,0,0,0,0,0,0,1,0,0,0,0,0,0, + +1,1,1,0,0,0,0,1,0,1,0,1,0,0,0,0,0, + +1,1,0,1,0,0,1,0,1,0,1,0,1,0,0,0,0, + +0,1,1,1,0,1,0,0,1,1,0,0,0,1,0,0,0, + +1,0,1,1,0,1,0,1,0,0,1,0,0,0,1,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ + DATA MAT3/ + + 36, 0, 0, 0, 9, 0,18,18, 0, 0,18,12,12, 0,12, 9, 0, + + 0,36, 0, 0, 9, 0,18, 0,18,18, 0,12,12,12, 0, 9, 0, + + 0, 0,36, 0, 9,18, 0,18, 0,18, 0,12, 0,12,12, 9, 0, + + 0, 0, 0,36, 9,18, 0, 0,18, 0,18, 0,12,12,12, 9, 0, + + 9, 9, 9, 9,36,18,18,18,18,18,18,27,27,27,27,36, 0, + + 0, 0,18,18,18,36, 0, 9, 9, 9, 9, 6, 6,12,12,18, 0, + + 18,18, 0, 0,18, 0,36, 9, 9, 9, 9,12,12, 6, 6,18, 0, + + 18, 0,18, 0,18, 9, 9,36, 0, 9, 9,12, 6, 6,12,18, 0, + + 0,18, 0,18,18, 9, 9, 0,36, 9, 9, 6,12,12, 6,18, 0, + + 0,18,18, 0,18, 9, 9, 9, 9,36, 0,12, 6,12, 6,18, 0, + + 18, 0, 0,18,18, 9, 9, 9, 9, 0,36, 6,12, 6,12,18, 0, + + 12,12,12, 0,27, 6,12,12, 6,12, 6,36, 8, 8, 8,27, 0, + + 12,12, 0,12,27, 6,12, 6,12, 6,12, 8,36, 8, 8,27, 0, + + 0,12,12,12,27,12, 6, 6,12,12, 6, 8, 8,36, 8,27, 0, + + 12, 0,12,12,27,12, 6,12, 6, 6,12, 8, 8, 8,36,27, 0, + + 9, 9, 9, 9,36,18,18,18,18,18,18,27,27,27,27,36, 0, + + 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0/ + DATA MAT4/ + + 0,0,2,1,0, + + 0,0,0,2,0, + + 2,0,0,0,0, + + 1,2,0,0,0, + + 0,0,0,0,0/ + DATA PAAS/'F','F','L','L','-','S','S','S','S','S', + 1'Y','Y','*','*','-','C','C','*','W','-', + 1'-','-','-','-','-','L','L','L','L','L', + 1'P','P','P','P','P','H','H','Q','Q','-', + 1'R','R','R','R','R','-','-','-','-','-','I','I','I','M','-', + 1'T','T','T','T','T', + 1'N','N','K','K','-','S','S','R','R','-','-','-','-','-','-', + 1'V','V','V','V','V','A','A','A','A','A','D','D','E','E','-', + 1'G','G','G','G','G', + 1'-','-','-','-','-','-','-','-','-','-', + 1'-','-','-','-','-','-','-','-','-','-', + 1'-','-','-','-','-','-','-','-','-','-'/ + DATA PAA/'F','F','L','L','-','S','S','S','S','S', + 1'Y','Y','*','*','-','C','C','*','W','-', + 1'-','-','-','-','-','L','L','L','L','L', + 1'P','P','P','P','P','H','H','Q','Q','-', + 1'R','R','R','R','R','-','-','-','-','-','I','I','I','M','-', + 1'T','T','T','T','T', + 1'N','N','K','K','-','S','S','R','R','-','-','-','-','-','-', + 1'V','V','V','V','V','A','A','A','A','A','D','D','E','E','-', + 1'G','G','G','G','G', + 1'-','-','-','-','-','-','-','-','-','-', + 1'-','-','-','-','-','-','-','-','-','-', + 1'-','-','-','-','-','-','-','-','-','-'/ +C Initialise help + CALL INTHLP('nip', TOPOPT) +C GET DEVICE NUMBERS + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + CALL OPENGR(DEVNOS(3)) + IFORNO = 0 + LIBNO = 1 + IGORT = 0 +C CALL CLEARV + WRITE(KBOUT,1000) +1000 FORMAT( + +' NIP (Nucleotide interpretation program) V7.0 July 1992',/, + +' Author: Rodger Staden'/) +C READ IN THE POINTERS TO THE HELP FILE + CALL SETHLP(HELPS,HELPE,BOTOPT,TOPOPT,POINTF,DEVNOS(4),KBOUT) + CALL INITGR(KBIN,KBOUT,HELPS(0),HELPE(0),HELPF,DEVNOS(4)) + IOK=0 + CALL INITLU(IDM) + CALL SETPAR(IOK) + IF(IOK.NE.0) GO TO 9999 +C GET SCREEN AND MARGIN SIZES + CALL GETMRG(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + +MAXOPT,DEVNOS(1),FILMAR) + IDEV=KBOUT + MOPT=0 + IDIMT = 0 +2 CONTINUE + IDEVLL = DEVNOS(5) + IDEVEN = DEVNOS(6) + IDEVAN = DEVNOS(7) + IDEVDL = DEVNOS(8) + IDEVLF = DEVNOS(9) + CALL RDSEQ( + +SEQ(2+MXSPAN/2),MAXSEQ,IDIMT,J1,J2,ISTART,IEND,IDIM1,IDIMB, + +DEVNOS(1),FILNAM,KBIN,KBOUT, + +HELPS(3),HELPE(3),HELPF,DEVNOS(4),IDEV,IFORNO, + +IDEVLL,IDEVEN,IDEVAN,IDEVDL, + +IDEVLF,LIBNO,LIBLF,WORKI,MAXWIR,IOK) +C SAVE FILE NAME + FILEIN=FILNAM + IF(IOK.NE.0)GO TO 1 + CALL SHOWFI(KBOUT,FILEIN) +C GIVE COMPOSITION AS A CHECK + IF(IDIMB.GT.0)CALL BCOMP(SEQ(2+MXSPAN/2),IDIMB,J1,J2,ISTART, + +KBOUT) +C set pointers to sequence: all this stuff relates to buffering sequences +C in disk files, which is no longer done. As it was such a pain to put in +C and may become useful again it is being left, although newer functions +C cannot be sure of conforming. +******** +C MAXSEQ = THE DIMENSION OF THE RAM BUFFER SEQ +C IDIMT = THE ACTUAL SEQUENCE LENGTH (AND THEREFORE THE NUMBER OF ELEMENTS +C IN THE DISK BUFFER) +C ISTART = THE SEQUENCE NUMBER OF THE CHARACTER OCCUPYING SEQ(1) +C J1 = THE SEQUENCE NUMBER OF THE FIRST CHARACTER IN THE ACTIVE REGION +C J2 = THE SEQUENCE NUMBER OF THE LAST CHARACTER IN THE ACTIVE REGION +C IDIM1 = J2-J1+1 I.E. THE NUMBER OF ELEMENTS IN THE ACTIVE REGION +C IEND = THE SEQUENCE NUMBER OF THE LAST ELEMENT OF SEQ +C IDIMB = IEND-ISTART+1 I.E. THE NUMBER OF ELEMENTS IN THE RAM BUFFER +******** + IDIM1P=IDIMB+MXSPAN +C need array size divisible by 3 for translation + IDIM3=3*((IDIMB+3)/3) +1 CONTINUE + CALL BPAUSE(KBIN,KBOUT,IOK) +C IF(IOK.NE.0)GO TO 9999 +C give menu, get option +C + CALL MENU(OPT,KOPT,MOPT,MAXOPT,MAXMEN,KBIN,KBOUT, + +HELPS(0),HELPE(0),HELPF,DEVNOS(4)) +C +C change region +C + IF((OPT.EQ.4).AND.(IDIMT.GT.0))THEN +C CALL REDEFA +C + (SEQ(2+MXSPAN/2),IDIMT,J1,J2,MAXSEQ,IDIM1,ISTART,IEND,IDIMB, +C + DEVNOS(5),KBIN,KBOUT, +C + IOK,SEQW,LREC,NREC, +C + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),MACTYP,MACSHT,MACLNG) + CALL GTREG(KBIN,KBOUT,ISTART,IEND,J1,J2, + + 'Define active region', + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),IOK) + IDIM1 = J2 - J1 + 1 + GO TO 1 + END IF +C +C STOP +C + IF(OPT.EQ.2)GO TO 9999 +C +C LIST +C + IF((OPT.EQ.5).AND.(IDIMB.GT.0))THEN + CALL LSTSEQ(SEQ(2+MXSPAN/2),IDIMB,IDEV,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),ISTART,IEND,IDIMT,KOPT) + GO TO 1 + END IF +C +C codon usage method to find genes +C + IF((OPT.EQ.42).AND.(IDIM1.GT.0))THEN + CALL PCODUS( + + SEQ,IDIM1P,IEND,MXSPAN, + + ISXMAX,ISYMAX,J1,J2,ISTART,MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + DEVNOS(1),FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),PAA) + GO TO 1 + END IF +C +C fickett +C + IF((OPT.EQ.48).AND.(IDIM1.GT.0)) THEN + CALL FICKET(SEQ,IDIM1P,IDIM1,MXSPAN, + + ISXMAX,ISYMAX,MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + J1-ISTART+1,J2-ISTART+1,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C dinucfreq +C + IF((OPT.EQ.22).AND.(IDIM1.GT.0))THEN + CALL DINUCF(SEQ(2+MXSPAN/2),IDIMB, + + J1-ISTART+1,J2-ISTART+1,IDEV,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C BASE COMPOSITION +C + IF((OPT.EQ.21).AND.(IDIM1.GT.0))THEN + CALL BCOMP(SEQ(2+MXSPAN/2),IDIMB, + + J1,J2,ISTART,IDEV) + GO TO 1 + END IF +C +C WRITE OUT ACTIVE SEQUENCE +C + IF((OPT.EQ.8).AND.(IDIM1.GT.0))THEN + CALL WRTACT(DEVNOS(1),FILNAM,KBIN,KBOUT, + + SEQ(J1+1-ISTART+1+MXSPAN/2),IDIM1, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C ruler +C + IF((OPT.EQ.12).AND.(IDIM1.GT.0))THEN + CALL RULER(J1,J2,MARGL,MARGR, + + MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,KBIN,KBOUT,1, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C composition +C + IF((OPT.EQ.24).AND.(IDIM1.GT.0))THEN + CALL COMPN(SEQ,IDIM1P,MXSPAN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1-ISTART+1,J2-ISTART+1,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C composition chi +C + IF((OPT.EQ.25).AND.(IDIM1.GT.0))THEN + CALL CCHI(SEQ,IDIM1P,MXSPAN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1,J2,ISTART,IEND,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),WORKR,KOPT) + GO TO 1 + END IF +C +C dichi +C + IF((OPT.EQ.26).AND.(IDIM1.GT.0))THEN + CALL DICHI(SEQ,IDIM1P,MXSPAN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1,J2,ISTART,IEND,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),WORKR,KOPT) + GO TO 1 + END IF +C +C trichi +C + IF((OPT.EQ.27).AND.(IDIM1.GT.0))THEN + CALL TRICHI(SEQ,IDIM1P,MXSPAN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1,J2,ISTART,IEND,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),WORKR,KOPT) + GO TO 1 + END IF +C +C negentropy +C + IF((OPT.EQ.59).AND.(IDIM1.GT.0))THEN + CALL NEGENT(SEQ,IDIM1P,MXSPAN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1,J2,ISTART,IEND,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),WORKR,KOPT) + GO TO 1 + END IF +C +C ribosomes (prokaryotic) +C + IF((OPT.EQ.58).AND.(IDIM1.GT.0))THEN + CALL PRIBS(SEQ(2+MXSPAN/2),IDIMB, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + DEVNOS(1),FILE4,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C clear all +C + IF(OPT.EQ.10) THEN + CALL CLEARG + GO TO 1 + END IF +C +C xhairs +C + IF((OPT.EQ.13).AND.(IDIM1.GT.0))THEN + XMAX=J2 + XMIN=J1 + YMAX=ISYMAX + YMIN=0. + IIIIX=0 + IIIIY=0 + CALL CLEARV + CALL XHAIRN(XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + ISXMAX,ISYMAX,IIIIX,IIIIY,N,KBOUT, + + SEQ(2+MXSPAN/2),ISTART,IDIMB, + + SEQ(2+MXSPAN/2),ISTART,IDIMB,1) + GO TO 1 + END IF +C +C clear vt100 +C + IF(OPT.EQ.11)THEN + CALL CLEARV + GO TO 1 + END IF +C +C CHANGE MARGINS +C + IF(OPT.EQ.14)THEN + CALL MARGC(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + + HELPS(OPT),HELPE(OPT),MAXOPT,HELPF,DEVNOS(4),KBIN,KBOUT) + GO TO 1 + END IF +C +C reverse +C + IF((OPT.EQ.29).AND.(IDIMB.GT.0))THEN + WRITE(KBOUT,4001)J1,J2 +4001 FORMAT(' Reverse and complement sequence from',I7,' to',I7) + CALL SQREV(SEQ(2+(MXSPAN/2)-ISTART+J1),IDIM1) + CALL SQCOM(SEQ(2+(MXSPAN/2)-ISTART+J1),IDIM1) + GO TO 1 + END IF +C +C translation list +C +C IF((OPT.EQ.38).AND.(IDIMB.GT.0))THEN +C CALL TRAN3(SEQ(2+MXSPAN/2),IDIMB,SEQW,IDIM3, +C + IDEV,DEVNOS(1),FILNAM,KBIN,KBOUT, +C + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),ISTART,IEND,IDIMT, +C + PAA) +C GO TO 1 +C END IF +C +C Analyse frequencies of repeats +C + IF((OPT.EQ.38).AND.(IDIM1.GT.0))THEN + CALL SQTREE(SEQ(J1-ISTART+2+MXSPAN/2),WORKI,IDIM1, + + WORDP,MAXDIC,WORKI(IDIM1+1),MAXWIR-IDIM1+1, + + ENZYME,MAXEN8,IDM,ISTART, + + IDEV,KBIN,KBOUT,HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C translation to disk +C + IF((OPT.EQ.40).AND.(IDIM1.GT.0))THEN + CALL TRANDK(SEQ(2+MXSPAN/2),IDIMB,SEQW,MAXSEQ, + + ISTART,IEND,IDIMT, + + DEVNOS(1),DEVNOS(8),FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),PAA,J1,J2, + + WORKI,MAXSEQ) + GO TO 1 + END IF +C +C longest open frame +C + IF((OPT.EQ.54).AND.(IDIM1.GT.0))THEN + CALL OPENFR(SEQ(2+MXSPAN/2),IDIMB,J1,J2,ISTART, + + IDEV,KBIN,KBOUT,PAA, + + SEQW,MAXSEQ, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C codons and base composition and molecular weights +C + IF((OPT.EQ.23).AND.(IDIMB.GT.0))THEN + CALL CODONS(SEQ(2+MXSPAN/2),IDIMB,J1,J2,IDEV, + + DEVNOS(1),FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),PAA,WORKI,MAXSEQ,SEQW) + GO TO 1 + END IF +C +C shepherd +C + IF((OPT.EQ.47).AND.(IDIM1.GT.0)) THEN + CALL SHEPED( + + SEQ,IDIM1P,IDIM1,MXSPAN, + + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1,MARGL,MARGR, + + MARGB(OPT),MARGT(OPT),KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),PAA,KOPT) + GO TO 1 + END IF +C +C positional base preferences for average amino acid composition +C + IF((OPT.EQ.43).AND.(IDIM1.GT.0)) THEN + CALL PBASEP( + + SEQ,IDIM1P,MXSPAN, + + ISXMAX,ISYMAX,J1,J2,ISTART,IEND, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),PAA,DEVNOS(1), + + FILNAM,KOPT) + GO TO 1 + END IF +C +C hairpins +C + IF((OPT.EQ.30).AND.(IDIM1.GT.0))THEN + CALL HAIRPN(SEQ(2+MXSPAN/2),IDIMB,WORKI(1), + + WORKI(1+MAXD3), + + WORKI(1+2*MAXD3),MAXD3,MARGL,MARGR,MARGB(OPT), + + MARGT(OPT),ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + ISTART,IDEV,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C plot map +C + IF((OPT.EQ.16).AND.(IDIM1.GT.0))THEN + CALL PLTMAQ(DEVNOS(1),FILNAM,WORKI,MAXSEQ,MARGL,MARGR, + + MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,J1,J2,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C reading frames using codon improbability expecting codon frequencies +C to depend on base composition +C + IF((OPT.EQ.45).AND.(IDIM1.GT.0)) THEN + CALL IMPBC( + + SEQ,IDIM1P,IDIM1,MXSPAN,WORKR, + + IDIM1P,ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + MARGL,MARGR,MARGB(OPT), + + MARGT(OPT),KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),PAA,KOPT) + GO TO 1 + END IF +C +C new file +C + IF(OPT.EQ.3)GO TO 2 +C +C SPLICE JUNCTIONS +C + IF((OPT.EQ.62).AND.(IDIM1.GT.0)) THEN +C CALL SPLIC1( +C + SEQ(2+MXSPAN/2),IDIMB, +C + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, +C + MARGL,MARGR,MARGB(OPT),MARGT(OPT), +C + DEVNOS(1), +C + FILE2,KBOUT) +C GO TO 1 + CALL FSPLIC( + + SEQ(2+MXSPAN/2),IDIMB,MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + DEVNOS(1),FILE2,FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),IDEV, + + WORKI,WORKR(1+MXWTLN*IDM),WORKR(1+2*MXWTLN*IDM), + + WORKI(1+3*MXWTLN*IDM),WORKI(1+3*MXWTLN*IDM+MXWTLN), + + WORKI(1+3*MXWTLN*IDM+2*MXWTLN), + + WORKI(1+3*MXWTLN*IDM+3*MXWTLN), + + WORKI(1+3*MXWTLN*IDM+4*MXWTLN), + + IDM,MXWTLN,SEQW,CHRSET,KOPT) + GO TO 1 + END IF +C +HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) +C +C SPLICE JUNCTIONS AG,GT NOT OBLIGATORY +C +C IF((OPT.EQ.63).AND.(IDIM1.GT.0))CALL SPLIC2( +C +SEQ(2+MXSPAN/2),IDIMB, +C 1ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, +C +MARGL,MARGR,MARGB(OPT),MARGT(OPT), +C +DEVNOS(1),FILE2,KBOUT) +C +HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) +C SPLICE JUNCTIONS AG,GT REMOVED +C +C IF((OPT.EQ.64).AND.(IDIM1.GT.0))CALL SPLIC3( +C +SEQ(2+MXSPAN/2),IDIMB, +C 1ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, +C +MARGL,MARGR,MARGB(OPT),MARGT(OPT), +C +DEVNOS(1),FILE2,KBOUT) +C +HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) +C +C signal searches from named plot files +C + IF((OPT.EQ.63).AND.(IDIM1.GT.0))THEN + CALL SIGNAL( + + SEQ(2+MXSPAN/2),IDIMB,MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + DEVNOS(1),FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),IDEV, + + WORKI,WORKR(1+MXWTLN*IDM),WORKI(1+2*MXWTLN*IDM), + + IDM,MXWTLN,SEQW,CHRSET,KOPT,2) + GO TO 1 + END IF +C +C PLOT WORD FREQ/ EXPECTED WORD FREQ +C + IF((OPT.EQ.64).AND.(IDIM1.GT.0))THEN + CALL WORDFP(SEQ,IDIM1P,IDIMB,MXSPAN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1,J2,ISTART,IEND,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C PROKARYOTIC PROMOTERS +C + IF((OPT.EQ.55).AND.(IDIM1.GT.0)) THEN + CALL PPROM1( + + SEQ(2+MXSPAN/2),IDIMB, + + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + DEVNOS(1),FILE3,KBOUT) + GO TO 1 + END IF +C +HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) +C +C PROKARYOTIC PROMOTERS ON COMPLEMENTARY STRAND +C + IF((OPT.EQ.56).AND.(IDIM1.GT.0)) THEN + CALL PPROM3( + + SEQ(2+MXSPAN/2),IDIMB, + + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + DEVNOS(1),FILE3,KBOUT) + GO TO 1 + END IF +C +HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) +C +C PROKARYOTIC PROMOTERS -35 AND -10 SEPARATELY +C + IF((OPT.EQ.57).AND.(IDIM1.GT.0)) THEN + CALL PPROM2( + + SEQ(2+MXSPAN/2),IDIMB, + + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + DEVNOS(1),FILE3,KBOUT) + GO TO 1 + END IF +C +HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) +C +C ribosomes (eukaryotic) +C + IF((OPT.EQ.61).AND.(IDIM1.GT.0)) THEN + CALL RIBEUK( + + SEQ(2+MXSPAN/2),IDIMB, + + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + DEVNOS(1),FILE1,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C polyA +C + IF((OPT.EQ.65).AND.(IDIM1.GT.0)) THEN + CALL SRCHP( + + SEQ(2+MXSPAN/2), + + IDIMB,MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1,KBOUT) + GO TO 1 + END IF +C +C type text +C + IF(OPT.EQ.6) THEN + CALL TTEXT(DEVNOS(1),FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C help +C + IF(OPT.EQ.1) THEN + CALL HELP(HELPS,HELPE,BOTOPT,TOPOPT,HELPF,DEVNOS(4), + + KBIN,KBOUT) + GO TO 1 + END IF +C +C start codons +C + IF((OPT.EQ.50).AND.(IDIM1.GT.0))THEN + CALL CLEARV + CALL STARTS(SEQ(2+MXSPAN/2),IDIMB,J1-ISTART+1,J2-ISTART+1, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,PAA) + GO TO 1 + END IF +C +C stop codons +C + IF((OPT.EQ.51).AND.(IDIM1.GT.0))THEN + CALL CLEARV + CALL STOPS(SEQ(2+MXSPAN/2),IDIMB,J1-ISTART+1,J2-ISTART+1, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,PAA) + GO TO 1 + END IF +C +C stop codons complementary +C + IF((OPT.EQ.52).AND.(IDIM1.GT.0))THEN + CALL CLEARV + CALL STOPSC(SEQ(2+MXSPAN/2),IDIMB,J1-ISTART+1,J2-ISTART+1, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,PAA) + GO TO 1 + END IF +C +C stop codons complementary and normal +C + IF((OPT.EQ.53).AND.(IDIM1.GT.0))THEN + CALL CLEARV + CALL STOPSB(SEQ(2+MXSPAN/2),IDIMB,J1-ISTART+1,J2-ISTART+1, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + ISXMAX,ISYMAX,PAA) + GO TO 1 + END IF +C +C codon improbability +C + IF((OPT.EQ.28).AND.(IDIMB.GT.0))THEN + CALL CODIMP(SEQ(2+MXSPAN/2),IDIMB,WORKR,IDIMB,DEVNOS(1), + + FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),J1,J2,IDEV, + = WORKI(101),MAXSEQ-101,SEQW) + GO TO 1 + END IF +C +C REPEATS +C + IF((OPT.EQ.32).AND.(IDIM1.GT.0))THEN + IF(IDIM1.LE.MAXWIR)THEN + CALL PREPT1( + + SEQ(2+MXSPAN/2),WORKI,IDIMB,J1,J2,ISTART, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),IDEV,KOPT, + + WORDP,WORDN,MAXWRD,MAXDIC) + ELSE + WRITE(KBOUT,1001)MAXWIR +1001 FORMAT(' Maximum length of active region for this option=', + + I7) + END IF + GO TO 1 + END IF +C +C codons to disk +C + IF((OPT.EQ.41).AND.(IDIMB.GT.0)) THEN + CALL CODTDK( + + SEQ(2+MXSPAN/2),IDIMB,J1,J2, + + DEVNOS(1),FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),PAA,WORKI,MAXSEQ,SEQW) + GO TO 1 + END IF +C +C zdna +C + IF((OPT.EQ.33).AND.(IDIM1.GT.0))THEN + CALL ZDNA(SEQ,IDIM1P,MXSPAN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1-ISTART+1,J2-ISTART+1,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C zdna runs of RY or YR +C + IF((OPT.EQ.34).AND.(IDIM1.GT.0)) THEN + CALL ZDNARN( + + SEQ(2+MXSPAN/2),IDIMB, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1-ISTART+1,J2-ISTART+1,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C zdnaROD +C + IF((OPT.EQ.35).AND.(IDIM1.GT.0))THEN + CALL ZDNARD(SEQ,IDIM1P,MXSPAN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1-ISTART+1,J2-ISTART+1,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C base phasing due to coding +C + IF((OPT.EQ.44).AND.(IDIM1.GT.0)) THEN + CALL BPHASE( + + SEQ,IDIM1P,IDIM1,MXSPAN, + + ISXMAX,ISYMAX,MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + J1-ISTART+1,J2-ISTART+1,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C trna +C + IF((OPT.EQ.49).AND.(IDIM1.GT.0)) THEN + CALL TRNA(SEQ(2+MXSPAN/2),IDIMB,J1-ISTART+1,J2-ISTART+1, + + ISTART, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + IDEV,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C REPEATS +C + IF((OPT.EQ.31).AND.(IDIM1.GT.0))THEN + IF(IDIM1.LE.MAXWIR)THEN + CALL PREPT2( + + SEQ(2+MXSPAN/2),WORKI,IDIMB,J1,J2,ISTART, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),IDEV,KOPT, + + WORDP,WORDN,MAXWRD,MAXDIC) + ELSE + WRITE(KBOUT,1001)MAXWIR + END IF + GO TO 1 + END IF +C +C reading frame search using codon improbability expecting even codons per +C acid +C + IF((OPT.EQ.46).AND.(IDIM1.GT.0)) THEN + CALL IMPAC( + + SEQ,IDIM1P,IDIM1,MXSPAN,WORKR, + + IDIM1P,ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),PAA,KOPT) + GO TO 1 + END IF +C +C new search +C + IF((OPT.EQ.17).AND.(IDIM1.GT.0)) THEN + KOPT = 1 + CALL SEARCH(SEQ(2+MXSPAN/2),IDIMB,J1,J2,ISTART,SEQW,MAXSD3, + + SEQW(1+MAXSD3),MAXSD3, + + SEQW(1+2*MAXSD3),MAXSD3, + + ENZYM1,ENZYM2,ENZYM3,ENZYM4,MAXNZ1, + + ENZYME,ENZYME(1+MAXEN),ENZYME(1+2*MAXEN),ENZYME(1+3*MAXEN), + + ENZYME(1+4*MAXEN),ENZYME(1+5*MAXEN),ENZYME(1+6*MAXEN), + + ENZYME(1+7*MAXEN),MAXEN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,IDEV, + + DEVNOS(1),FILNAM,FILE5,FILE6,FILE7,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),PAA,WORKI,WORDP,MAXDIC, + + KOPT) + GO TO 1 + END IF +C +C Translate and list 0-6 phases +C + IF((OPT.EQ.39).AND.(IDIM1.GT.0))THEN + CALL OPENTR(SEQ(2+MXSPAN/2),IDIMB,ISTART, + + IDEV,KBIN,KBOUT,PAA, + + SEQW,MAXSEQ,WORKI,WORKI(IDIMB+1),MAXSEQ-IDIMB, + + DEVNOS(1),FILNAM, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C GET DISK OUTPUT FILE ON UNIT DEVNOS(2) IF REQUIRED +C + IF(OPT.EQ.7)THEN + CALL REDIR(IDEV,DEVNOS(2),DEVNOS(3),IGORT,FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C signal searches from named plot files +C + IF((OPT.EQ.20).AND.(IDIM1.GT.0))THEN + CALL SIGNAL( + + SEQ(2+MXSPAN/2),IDIMB,MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + DEVNOS(1),FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),IDEV, + + WORKI,WORKR(1+MXWTLN*IDM),WORKI(1+2*MXWTLN*IDM), + + IDM,MXWTLN,SEQW,CHRSET,KOPT,1) + GO TO 1 + END IF +C +C SIGNAL SEARCH ON DINUCLEOTIDES +C + IF((OPT.EQ.60).AND.(IDIM1.GT.0)) THEN + CALL DISIG( + + SEQ(2+MXSPAN/2),IDIMB,MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + DEVNOS(1),FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),IDEV, + + WORKI,WORKR(1+MXWTLN*IDM*IDM),WORKI(1+2*MXWTLN*IDM*IDM), + + IDM,MXWTLN,SEQW,CHRSET,KOPT) + GO TO 1 + END IF +C +C SEQFIT +C + IF((OPT.EQ.18).AND.(IDIM1.GT.0))THEN + CALL SEQFIT(SEQ(2+MXSPAN/2),IDIMB,SEQW,MAXSEQ, + + WORKI,WORKI(1+MAXD2),MAXD2, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1,J2,ISTART,IDEV, + + DEVNOS(1),FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C SEPFIT +C + IF((OPT.EQ.19).AND.(IDIM1.GT.0))THEN + CALL SEPFIT(SEQ(2+MXSPAN/2),IDIMB,SEQW,MAXSEQ, + + WORKI,WORKI(1+MAXD2),MAXD2, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,J1,J2, + + ISTART,IDEV, + + DEVNOS(1),FILNAM,KBIN,KBOUT,MAT3,IDME, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C LOCAL SIMILARITY OR COMPLEMENTARITY +C + IF((OPT.EQ.36).AND.(IDIM1.GT.0))THEN + CALL LOCALF(SEQ(2+MXSPAN/2),IDIMB,SEQW,SEQW(MAXSD2+1),MAXSD2, + + WORKI,WORKI(1+MAXD2),MAXD2, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + ISXMAX,ISYMAX,J1,J2,ISTART, + + IDEV,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),DIALOG) + GO TO 1 + END IF +C +C SET GENETIC CODE +C + IF(OPT.EQ.37) THEN + CALL SETGEN(PAAS,PAA,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C WRITE LABELS +C + IF(OPT.EQ.15) THEN + CALL LABLER(KBIN,KBOUT,ISXMAX,ISYMAX, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C CHANGE DNA TO RNA OR RNA TO DNA +C + IF((OPT.EQ.66).AND.(IDIM1.GT.0))THEN + WRITE(KBOUT,1002)ISTART,IEND +1002 FORMAT(' Exchange T and U for the region',I7,' TO',I7) + CALL DNARNA(SEQ(2+MXSPAN/2),IDIMB) + GO TO 1 + END IF +C +C SHOW SETTINGS +C + IF(OPT.EQ.9)THEN + WRITE(KBOUT,4000)FILEIN,J1,J2,IDIM1 +4000 FORMAT(' Sequence=',A,/,' Start=',I7,' End=',I7, + +' LENGTH=',I7) +C THIS NEXT LINE IS BECAUSE SOMETIMES THE FILE IS NOT READY TO EDIT! + CLOSE(UNIT=DEVNOS(2)) + CALL SEQEDT(SEQ(2+MXSPAN/2),MAXSEQ,IDIMB,KBIN, + + KBOUT,HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),DEVNOS(1), + + FILNAM,FILEIN,IDM,IOK) + IF(IOK.NE.0) GO TO 1 +******** +C MAXSEQ = THE DIMENSION OF THE RAM BUFFER SEQ +C IDIMT = THE ACTUAL SEQUENCE LENGTH (AND THEREFORE THE NUMBER OF ELEMENTS +C IN THE DISK BUFFER) +C ISTART = THE SEQUENCE NUMBER OF THE CHARACTER OCCUPYING SEQ(1) +C J1 = THE SEQUENCE NUMBER OF THE FIRST CHARACTER IN THE ACTIVE REGION +C J2 = THE SEQUENCE NUMBER OF THE LAST CHARACTER IN THE ACTIVE REGION +C IDIM1 = J2-J1+1 I.E. THE NUMBER OF ELEMENTS IN THE ACTIVE REGION +C IEND = THE SEQUENCE NUMBER OF THE LAST ELEMENT OF SEQ +C IDIMB = IEND-ISTART+1 I.E. THE NUMBER OF ELEMENTS IN THE RAM BUFFER +C IDIM3 = A BUFFER SIZE USED BY TRANSLATION ROUTINE +C THIS IS A FUDGE AND ONLY WORKS FOR UNBUFFERED SEQUENCES!!!!!!!!!!! + J1 = 1 + J2 = IDIMB + IDIMT = IDIMB + IEND = IDIMB + IDIM1 = IDIMB + IDIM3=3*((IDIMB+3)/3) + WRITE(KBOUT,4000)FILEIN,J1,J2,IDIM1 + GO TO 1 + END IF +C +C PATTERN SEARCH +C + IF((OPT.EQ.67).AND.(IDIM1.GT.0))THEN + CALL PATTEN(SEQ(J1-ISTART+2+MXSPAN/2),IDIM1,SEQW,MAXSEQ, + + WORKI(1),WORKI(MAXMOT+1),WORKI(2*MAXMOT+1),WORKI(3*MAXMOT+1), + + WORKI(4*MAXMOT+1),WORKI(5*MAXMOT+1),WORKI(6*MAXMOT+1), + + WORKI(7*MAXMOT+1),WORKI(8*MAXMOT+1),WORKI(9*MAXMOT+1), + + WORKI(10*MAXMOT+1),WORKI(11*MAXMOT+1),WORKI(12*MAXMOT+1), + + WORKI(13*MAXMOT+1),WORKI(14*MAXMOT+1),WORKI(15*MAXMOT+1), + + WORKI(16*MAXMOT+1),WORKI(17*MAXMOT+1),WORKI(18*MAXMOT+1), + + WORKI(19*MAXMOT+1),WORKI(20*MAXMOT+1), + + WORKR(22*MAXMOT+1),WORKR(23*MAXMOT+1), + + FILNAM,MAXMOT,MAXWTS,IDEV,DEVNOS(6),DEVNOS(7), + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,J1, + + KBIN,KBOUT,DEVNOS(8),IDM,SEQ(1),IDME, + + MAT1,MAT2,MAT3,MAT4,NAMSAV,KEYNS, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),FOFNAM,DEVNOS(1)) +C NB IVE SENT THE START OF SEQ TO BE USED IN PATTEN. THIS IS OK +C AS LONG AS MAXMOTT + IF(0.EQ.0) RETURN +C WRITE(*,*)'D(1),M(B1,B2)',D(1),M(B1,B2) +C +C add in the observed mutation to the appropriate mutation rate +C which is equivalent to adding (1 - the corrections) +C + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X +C +C now add in the corrections for the barred bases +C + E(B1,C) = E(B1,C) + ADD*M(B1,C)/X + E(B1,A) = E(B1,A) + ADD*M(B1,A)/X + E(B1,G) = E(B1,G) + ADD*M(B1,G)/X + ELSE IF (MC.EQ.2) THEN + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + E(B1,A) = E(B1,A) + ADD*M(B1,A)/X + E(B1,G) = E(B1,G) + ADD*M(B1,G)/X + ELSE IF (MC.EQ.3) THEN +C WRITE(*,*)'D(3),M(B1,B2)',D(3),M(B1,B2) + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + E(B1,G) = E(B1,G) + ADD*M(B1,G)/X + ELSE IF (MC.EQ.4) THEN +C T>TCAG +C WRITE(*,*)'D(),M(B1,B2)',D(4),M(B1,B2) + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + ELSE IF (MC.EQ.5) THEN +C C>C + IF(0.EQ.0) RETURN + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + E(B1,T) = E(B1,T) + ADD*M(B1,T)/X + E(B1,A) = E(B1,A) + ADD*M(B1,A)/X + E(B1,G) = E(B1,G) + ADD*M(B1,G)/X + ELSE IF (MC.EQ.6) THEN +C C>CT + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + E(B1,A) = E(B1,A) + ADD*M(B1,A)/X + E(B1,G) = E(B1,G) + ADD*M(B1,G)/X + ELSE IF (MC.EQ.7) THEN +C C>TCAG + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + ELSE IF (MC.EQ.8) THEN +C A>A + IF(0.EQ.0) RETURN +C WRITE(*,*)'D(8),M(B1,B2)',D(8),M(B1,B2) + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + E(B1,T) = E(B1,T) + ADD*M(B1,T)/X + E(B1,C) = E(B1,C) + ADD*M(B1,C)/X + E(B1,G) = E(B1,G) + ADD*M(B1,G)/X + ELSE IF (MC.EQ.9) THEN +C A>AG + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + E(B1,T) = E(B1,T) + ADD*M(B1,T)/X + E(B1,C) = E(B1,C) + ADD*M(B1,C)/X + ELSE IF (MC.EQ.10) THEN +C A>TCA + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + E(B1,G) = E(B1,G) + ADD*M(B1,G)/X + ELSE IF (MC.EQ.11) THEN +C A>TCAG + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + ELSE IF (MC.EQ.12) THEN +C G>G + IF(0.EQ.0) RETURN +C WRITE(*,*)'D(12),M(B1,B2)',D(12),M(B1,B2) + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + E(B1,T) = E(B1,T) + ADD*M(B1,T)/X + E(B1,C) = E(B1,C) + ADD*M(B1,C)/X + E(B1,A) = E(B1,A) + ADD*M(B1,A)/X + ELSE IF (MC.EQ.13) THEN +C G>AG + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + E(B1,T) = E(B1,T) + ADD*M(B1,T)/X + E(B1,C) = E(B1,C) + ADD*M(B1,C)/X + ELSE IF (MC.EQ.14) THEN +C G>TCAG + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + ELSE IF (MC.EQ.15) THEN +C C>CA + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + E(B1,T) = E(B1,T) + ADD*M(B1,T)/X + E(B1,G) = E(B1,G) + ADD*M(B1,G)/X + ELSE IF (MC.EQ.16) THEN +C C>TCA + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + E(B1,G) = E(B1,G) + ADD*M(B1,G)/X + ELSE IF (MC.EQ.17) THEN +C A>CA + E(B1,B2) = E(B1,B2) + ADD*M(B1,B2)/X + E(B1,T) = E(B1,T) + ADD*M(B1,T)/X + E(B1,G) = E(B1,G) + ADD*M(B1,G)/X + ELSE + WRITE(*,*)'SCREAM UPDM',MC + END IF + END + SUBROUTINE UPDE(M,E,MC,SUMS,PAA) + REAL M(4,4),D(17),E(4,4),T(4),PREV(4,4),DELTA + INTEGER SUMS(4,4,4,4,4,4),MC(4,4,4,3),CYCLE,ND(17) + CHARACTER PAA(5,5,5) + PARAMETER (SMALL = 1E-10) + SAVE DELTA + DATA DELTA/0.01/ + DO 1 I=1,4 + DO 1 J=1,4 + PREV(I,J) = M(I,J) + 1 CONTINUE + DO 3 I=1,17 + ND(I) = 0 + 3 CONTINUE + IR = 0 + 5 CONTINUE + IR = IR + 1 + CALL MAKED(M,D) + DO 10 I=1,4 + DO 10 J=1,4 + E(I,J) = 0. + 10 CONTINUE + DO 20 I=1,4 + DO 20 J=1,4 + DO 20 K=1,4 + DO 15 II=1,4 + DO 15 JJ=1,4 + DO 15 KK=1,4 + IF(PAA(K,J,I).EQ.PAA(KK,JJ,II)) THEN + IF((I.NE.II).OR.(J.NE.JJ).OR.(K.NE.KK)) THEN + CALL UPDM(M,D,E,MC(K,J,I,1),I,II,SUMS(I,J,K,II,JJ,KK),ND) + CALL UPDM(M,D,E,MC(K,J,I,2),J,JJ,SUMS(I,J,K,II,JJ,KK),ND) + CALL UPDM(M,D,E,MC(K,J,I,3),K,KK,SUMS(I,J,K,II,JJ,KK),ND) + END IF + END IF + 15 CONTINUE + 20 CONTINUE +C WRITE(*,*)'NEW E ' +C DO 55 I=1,4 +C WRITE(*,*)(E(I,J),J=1,4) +C 55 CONTINUE + CALL FILLR(T,4,SMALL) + DO 30 I=1,4 + DO 30 J=1,4 + T(I) = T(I) + E(I,J) + 30 CONTINUE +C WRITE(*,*)'ROW TOTS',T + DO 40 I=1,4 + DO 40 J=1,4 + M(I,J) = E(I,J)/T(I) + 40 CONTINUE +C WRITE(*,*)' NEW M' +C DO 50 I=1,4 +C WRITE(*,1000)(M(I,J),J=1,4) +C WRITE(*,1000)(PREV(I,J),J=1,4) +C 50 CONTINUE +C 1000 FORMAT(4F8.5) + CYCLE = 0 + DO 60 I=1,4 + DO 60 J=1,4 + IF (ABS(M(I,J)-PREV(I,J)).GT.DELTA) CYCLE = 1 +C PREV(I,J) = M(I,J) + 60 CONTINUE +C WRITE(*,*)IR + DO 70 I=1,4 + DO 70 J=1,4 + PREV(I,J) = M(I,J) + 70 CONTINUE + IF(CYCLE.EQ.1) GO TO 5 + WRITE(*,*)'Number of iterations',IR +C WRITE(*,*)(K,ND(K),K=1,17) + END + SUBROUTINE UPDET(SUMS,PAA,MC) + INTEGER SUMS(4,4,4,4,4,4),MC(4,4,4,3) + CHARACTER PAA(5,5,5),BASES(4) + SAVE BASES + DATA BASES/'T','C','A','G'/ + DO 20 I=1,4 + DO 20 J=1,4 + DO 20 K=1,4 + DO 15 II=1,4 + DO 15 JJ=1,4 + DO 15 KK=1,4 + IF(PAA(K,J,I).EQ.PAA(KK,JJ,II)) THEN +C IF(PAA(I,J,K).EQ.PAA(II,JJ,KK)) THEN +C WRITE(*,*)I,J,K,' to',II,JJ,KK,SUMS(I,J,K,II,JJ,KK) + WRITE(*,*)BASES(I),BASES(J),BASES(K), + +BASES(II),BASES(JJ),BASES(KK),MC(K,J,I,1),MC(K,J,I,2), + +MC(K,J,I,3),SUMS(I,J,K,II,JJ,KK) + END IF + 15 CONTINUE + 20 CONTINUE + END + SUBROUTINE COCHN4(SEQ1,IDIM1,I1,N1,SEQ2,IDIM2,I2,N2,CHANGE) + CHARACTER SEQ1(IDIM1),SEQ2(IDIM2) + INTEGER CHANGE(4,4,4,4,4,4),CTONUM,K1(3),K2(3) + EXTERNAL CTONUM + IDIM3=N1-I1+1 + IDIM4=N2-I2+1 +C GET MINIMUM LENGTH TO SCAN + MINL=MIN(IDIM3,IDIM4) + J1=I1 - 3 + J2 = I2 - 3 + DO 10 I=1,MINL-2,3 + J1 = J1 + 3 + J2 = J2 + 3 + DO 5 JJ=1,3 + KK = CTONUM(SEQ1(J1+JJ-1)) + IF(KK.EQ.5) GO TO 10 + K1(JJ) = KK + KK = CTONUM(SEQ2(J2+JJ-1)) + IF(KK.EQ.5) GO TO 10 + K2(JJ) = KK + 5 CONTINUE +C WRITE(*,*)I,K1,K2 + CHANGE(K1(1),K1(2),K1(3),K2(1),K2(2),K2(3)) = + + CHANGE(K1(1),K1(2),K1(3),K2(1),K2(2),K2(3)) + 1 +10 CONTINUE + END + SUBROUTINE EMT(MTT) + INTEGER MTT(64,3) + CHARACTER MTTC(64)*3 + SAVE MTTC + DATA MTTC/ + +'112','116','219','21D', + +'154','157','15B','15E', + +'182','186','199','19D', + +'1C2','1C6','1D8','1CC', + +'514','517','61B','61E', + +'554','557','55B','55E', + +'582','586','589','58D', + +'5C4','5C7','FCB','FCE', + +'813','81G','81A','81C', + +'854','857','85B','85E', + +'882','886','889','88D', + +'8C2','8C6','HC9','HCD', + +'C14','C17','C1B','C1E', + +'C54','C57','C5B','C5E', + +'C82','C86','C89','C8D', + +'CC4','CC7','CCB','CCE'/ + CALL CTI(MTTC,MTT) +C CALL WRTCOI(SUM,6,PAA,MTTC) +C CALL EM(PAA,KBOUT) + END + SUBROUTINE CTI(MTTC,MTT) + CHARACTER MTTC(64)*3 + INTEGER MTT(64,3) + CHARACTER C*17 + DATA C/'123456789ABCDEFGH'/ + DO 10 J=1,64 + DO 8 M=1,3 + DO 5 K=1,17 + IF(MTTC(J)(M:M).EQ.C(K:K)) THEN + MTT(J,M) = K + GO TO 8 + END IF + 5 CONTINUE + 8 CONTINUE + 10 CONTINUE + END + SUBROUTINE WRTCOI(SUM,IDEV,PAA,MTT) +C AUTHOR: RODGER STADEN + INTEGER SUM(4,4,4) + CHARACTER BASE(4),PAA(5,5,5),MTT(4,4,4)*3 + SAVE BASE + DATA BASE/'T','C','A','G'/ +C + WRITE(IDEV,1001) +1001 FORMAT(6X,'===========================================') + DO 10 I=1,4 + DO 20 K=1,4 + WRITE(IDEV,1000)(PAA(K,J,I), + +BASE(I),BASE(J),BASE(K),SUM(I,J,K),J=1,4) + WRITE(IDEV,1002)(MTT(K,J,I),J=1,4) + 1002 FORMAT(5X,4(1X,1X,A,5X)) +20 CONTINUE +10 WRITE(IDEV,1001) +1000 FORMAT(5X,4(1X,A1,1X,3A1,I5)) + END diff --git a/src/staden/nipl.f b/src/staden/nipl.f new file mode 100644 index 0000000..f1afda5 --- /dev/null +++ b/src/staden/nipl.f @@ -0,0 +1,1207 @@ +C NIPL (Nucleotide interpretation program (library)) +C 2-12-92 upped maxseq, maxwir to 360,000 +C 9-7-92 added fasta format capability +C 29-3-1990 DSPLAZ thought repeat was class 5 corrected to 8 +C 11-4-1990 Changed title to be string rather than array +C 18-4-1990 Changed to allow use of library index when appropriate +C 8-11-90 Changed call to getmf for compatibility with nip +C and replaced all calls to radio by radion +C 11-12-90 Changed library handling so added filnll and a parameter +C 3-7-91 set namlen = 60 +C 4-7-91 inlined weight matrix search (copied from pipl). Should do rest! +C 18-7-91 Added titles to pattern files +C 16-12-91 Added access to pir library in codata form to pipl so make this the same +C 2-3-92 set filnam = ' ' for some calls to openf1 + SUBROUTINE FMAIN() + INTEGER BOTOPT,TOPOPT + PARAMETER (NAMLEN = 60) + CHARACTER*(NAMLEN) FILNAM,HELPF,LIBLF + PARAMETER ( + + MAXSEQ=360000, + + MXSPAN=603, + + MAXWIN=MAXSEQ+MXSPAN, + + MAXWIR=360000, + + MAXD36=MAXWIR/36, + + MAXD2=MAXWIR/2, + + MAXD3=MAXWIR/3, + + MAXSD2=MAXSEQ/2, + + MAXSD3=MAXSEQ/3, + + MAXDEV=10, + + LIBLF = 'SEQUENCELIBRARIES', + + LENNAM = 10) + PARAMETER (MAXMOT = 50, + + MAXWTS = 5000, + + IDM = 5, + + IDME = 17) + INTEGER BESTP(MAXMOT),BESTQ(MAXMOT) + REAL BESTS(MAXMOT) + CHARACTER*(LENNAM) ENAMEL,ENAME,NAMSAV(MAXMOT) + PARAMETER (MAXDIV = 15) + INTEGER DIVDEV(MAXDIV),RSIZEN +C COMPATIBILITY WITH ANALYSEQ HELP STUFF + PARAMETER (BOTOPT=0,TOPOPT=1) + INTEGER HELPS(BOTOPT:TOPOPT),HELPE(BOTOPT:TOPOPT) + INTEGER DEVNOS(MAXDEV) + REAL WORKR(MAXWIR) + INTEGER WORKI(MAXWIR) + CHARACTER SEQ(MAXWIN),SEQW(MAXSEQ) +C MAT1 SIMPLE IDENTITY +C MAT2 IUB SCORES 0-1 +C MAT3 IUB SCORES 0-36 +C MAT4 INVERTED REPEAT + INTEGER MAT1(IDM,IDM),MAT2(IDME,IDME) + INTEGER MAT3(IDME,IDME),MAT4(IDM,IDM) + CHARACTER*8 KEYNS(MAXMOT) + CHARACTER LTYPE + EQUIVALENCE (WORKR,WORKI) + DATA MAT1/ + + 1,0,0,0,0, + + 0,1,0,0,0, + + 0,0,1,0,0, + + 0,0,0,1,0, + + 0,0,0,0,0/ + DATA MAT2/ + +1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,0,1,0,0,0,0,0,0,0,0,0,0,0, + +1,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0,0, + +1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0, + +0,1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0, + +0,1,1,0,0,0,0,0,0,1,0,0,0,0,0,0,0, + +1,0,0,1,0,0,0,0,0,0,1,0,0,0,0,0,0, + +1,1,1,0,0,0,0,1,0,1,0,1,0,0,0,0,0, + +1,1,0,1,0,0,1,0,1,0,1,0,1,0,0,0,0, + +0,1,1,1,0,1,0,0,1,1,0,0,0,1,0,0,0, + +1,0,1,1,0,1,0,1,0,0,1,0,0,0,1,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ + DATA MAT3/ + + 36, 0, 0, 0, 9, 0,18,18, 0, 0,18,12,12, 0,12, 9, 0, + + 0,36, 0, 0, 9, 0,18, 0,18,18, 0,12,12,12, 0, 9, 0, + + 0, 0,36, 0, 9,18, 0,18, 0,18, 0,12, 0,12,12, 9, 0, + + 0, 0, 0,36, 9,18, 0, 0,18, 0,18, 0,12,12,12, 9, 0, + + 9, 9, 9, 9,36,18,18,18,18,18,18,27,27,27,27,36, 0, + + 0, 0,18,18,18,36, 0, 9, 9, 9, 9, 6, 6,12,12,18, 0, + + 18,18, 0, 0,18, 0,36, 9, 9, 9, 9,12,12, 6, 6,18, 0, + + 18, 0,18, 0,18, 9, 9,36, 0, 9, 9,12, 6, 6,12,18, 0, + + 0,18, 0,18,18, 9, 9, 0,36, 9, 9, 6,12,12, 6,18, 0, + + 0,18,18, 0,18, 9, 9, 9, 9,36, 0,12, 6,12, 6,18, 0, + + 18, 0, 0,18,18, 9, 9, 9, 9, 0,36, 6,12, 6,12,18, 0, + + 12,12,12, 0,27, 6,12,12, 6,12, 6,36, 8, 8, 8,27, 0, + + 12,12, 0,12,27, 6,12, 6,12, 6,12, 8,36, 8, 8,27, 0, + + 0,12,12,12,27,12, 6, 6,12,12, 6, 8, 8,36, 8,27, 0, + + 12, 0,12,12,27,12, 6,12, 6, 6,12, 8, 8, 8,36,27, 0, + + 9, 9, 9, 9,36,18,18,18,18,18,18,27,27,27,27,36, 0, + + 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0/ + DATA MAT4/ + + 0,0,2,1,0, + + 0,0,0,2,0, + + 2,0,0,0,0, + + 1,2,0,0,0, + + 0,0,0,0,0/ +C GET DEVICE NUMBERS + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + WRITE(KBOUT,1000) +1000 FORMAT(/, + +' NIPL (Nucleotide interpretation program (Library))', + +' V4.1 Jul 1991',/, + +' Author: Rodger Staden',/, + +' Searches nucleotide libraries for patterns of motifs',/) + CALL INITLU(IDM) + FILNAM = ' ' + CALL OPENF1(DEVNOS(1),FILNAM,1,IOK,KBIN,KBOUT, + +'Name for results file', + +HELPS(1),HELPE(1),HELPF,DEVNOS(2)) + IF(IOK.NE.0)STOP +C OPEN LIBRARY + LIBIN = 1 + IDEVNL = DEVNOS(7) + IDEVLL = DEVNOS(8) + IDEVEN = DEVNOS(9) + IDEVD = DEVNOS(MAXDEV) + CALL RDLIBL(FILNAM,KBIN,KBOUT, + +HELPS(1),HELPE(1),HELPF,DEVNOS(2),IDEVLL,IDEVEN,IDEVNL, + +LIBLF,LIBIN,DIVDEV,MAXDIV,IDEVD, + +LIST,ENAMEL,LIBTYP,LTYPE,NDIV,RSIZEN,NRECEN,IOK) + IF(IOK.NE.0)STOP + IDEVOT=DEVNOS(1) + J1 = 1 + CALL PATTEO(SEQ(MXSPAN+1),MAXSEQ,SEQW,MAXSEQ, + + WORKI(1),WORKI(MAXMOT+1),WORKI(2*MAXMOT+1),WORKI(3*MAXMOT+1), + + WORKI(4*MAXMOT+1),WORKI(5*MAXMOT+1),WORKI(6*MAXMOT+1), + + WORKI(7*MAXMOT+1),WORKI(8*MAXMOT+1),WORKI(9*MAXMOT+1), + + WORKI(10*MAXMOT+1),WORKI(11*MAXMOT+1),WORKI(12*MAXMOT+1), + + WORKI(13*MAXMOT+1),WORKI(14*MAXMOT+1),WORKI(15*MAXMOT+1), + + WORKI(16*MAXMOT+1),WORKI(17*MAXMOT+1),WORKI(18*MAXMOT+1), + + WORKI(19*MAXMOT+1),WORKI(20*MAXMOT+1), + + WORKR(22*MAXMOT+1),WORKR(23*MAXMOT+1), + + FILNAM,MAXMOT,MAXWTS,IDEVOT,DEVNOS(3),DEVNOS(4),J1, + + KBIN,KBOUT,DEVNOS(5),IDM,SEQ(1),IDME, + + MAT1,MAT2,MAT3,MAT4,NAMSAV,KEYNS, + + WORKI(23*MAXMOT+1+MAXWTS), + + WORKI(23*MAXMOT+MAXWTS+3001),LIST, + + HELPS,HELPE,HELPF,DEVNOS(2),DEVNOS(6),ENAMEL,ENAME,LIBTYP, + + IDEVEN,RSIZEN,NRECEN,IDEVNL,DIVDEV,NDIV,BESTP,BESTQ,BESTS, + + LTYPE) +C NB HAVE SET MAXIMUM COMBINED STRING LENGTH TO 3000 ON PREVIOUS LINE +C AND MAX INTEGER VERSION OF SEQUENCE IS WHATEVER IS LEFT AND THIS IS NOT +C SENT OR CHECKED BY THE CODE !!!!!!!!!!!!!!!!!!!!!!!!!! +C +900 CONTINUE + END + SUBROUTINE PATTEO(SEQ,MAXSEQ,STRING,MAXSTR, + +LENGTH,CLASS,RELMOT,RANGES,RANGEL,RANGET,RANGEM,IENTRY, + +START2,IEND2,WTSTR,START,IEND,MATCHQ,RELEND,MATCHP, + +STRNGS,LAST5,LAST3S,LAST3E,MATCHS,CUTOFF,WEIGHT,FILNAM, + +MAXMOT,MAXWTS, + +IDEV1,IDEV2,IDEV3, + +KSTART,KBIN,KBOUT, + +IDEV4,IDM,COMBIN,IDME, + + MAT1,MAT2,MAT3,MAT4,NAMSAV,KEYNS,STRNGI,SEQI,LIST, + + IHELPS,IHELPE,HELPF,IDEVH,IDEVN,ENAMEL,NAMIN,LIBTYP, + + IDEVEN,RSIZEN,NRECEN,IDEVNL,DIVDEV,NDIV,BESTP,BESTQ,BESTS, + + LTYPE) + INTEGER DIVDEV(NDIV),RSIZEN + INTEGER LENGTH(MAXMOT),CLASS(MAXMOT),RELMOT(MAXMOT) + INTEGER RANGES(MAXMOT),RANGEL(MAXMOT) + INTEGER RANGET(MAXMOT),RANGEM(MAXMOT),IENTRY(MAXMOT) + INTEGER START2(MAXMOT),IEND2(MAXMOT) + CHARACTER SEQ(MAXSEQ),STRING(MAXSTR) + INTEGER WTSTR(MAXMOT),START(MAXMOT),IEND(MAXMOT) + INTEGER MATCHQ(MAXMOT),RELEND(MAXMOT) + INTEGER MATCHP(MAXMOT),STRNGS(MAXMOT) + INTEGER LAST5(MAXMOT),LAST3S(MAXMOT),LAST3E(MAXMOT) + REAL WEIGHT(MAXWTS),CUTOFF(MAXMOT),MATCHS(MAXMOT) + REAL MINSCR,MAXSCR + CHARACTER FILNAM*(*),NAMIN*(*),ENAMEL*(*),HELPF*(*) + CHARACTER*(*) KEYNS(MAXMOT),NAMSAV(MAXMOT) + CHARACTER COMBIN(MAXMOT),LTYPE +C NB PROBLEM ABOUT USING MAXSEQ AS DIMENSION!!!!!!!!!!!! + INTEGER STRNGI(MAXSTR),SEQI(MAXSEQ) + INTEGER BESTP(MAXMOT),BESTQ(MAXMOT),ENTRYN + REAL BESTS(MAXMOT) + CHARACTER TITLE*60,TITLEP*80 +C MAT1 SIMPLE IDENTITY +C MAT2 IUB SCORES 0-1 +C MAT3 IUB SCORES 0-36 +C MAT4 INVERTED REPEAT + INTEGER MAT1(IDM,IDM),MAT2(IDME,IDME) + INTEGER MAT3(IDME,IDME),MAT4(IDM,IDM) + REAL EXPECC(4) + PARAMETER (MAXCLS = 8) + PARAMETER (MAXPRM = 25) + CHARACTER PROMPT(5)*(MAXPRM) + SAVE EXPECC + DATA EXPECC/.25,.25,.25,.25/ + IDSEQ = 1000 +C ZERO ARRAYS +C + CALL FILLI(LENGTH,MAXMOT,0) + CALL FILLI(CLASS,MAXMOT,0) + CALL FILLI(RELMOT,MAXMOT,0) + CALL FILLI(RANGES,MAXMOT,0) + CALL FILLI(RANGEL,MAXMOT,0) + CALL FILLI(RANGET,MAXMOT,0) + CALL FILLI(RANGEM,MAXMOT,0) + CALL FILLI(IENTRY,MAXMOT,0) + CALL FILLI(START2,MAXMOT,0) + CALL FILLI(IEND2,MAXMOT,0) + CALL FILLI(WTSTR,MAXMOT,0) + CALL FILLI(START,MAXMOT,0) + CALL FILLI(IEND,MAXMOT,0) + CALL FILLI(MATCHQ,MAXMOT,0) + CALL FILLI(RELEND,MAXMOT,0) + CALL FILLI(MATCHP,MAXMOT,0) + CALL FILLI(STRNGS,MAXMOT,0) + CALL FILLI(LAST5,MAXMOT,0) + CALL FILLI(LAST3S,MAXMOT,0) + CALL FILLI(LAST3E,MAXMOT,0) + CALL FILLR(CUTOFF,MAXMOT,0.0) + CALL FILLR(MATCHS,MAXMOT,0.0) + CALL FILLR(WEIGHT,MAXWTS,0.0) + CALL FILLC(COMBIN,MAXMOT,'A') + CALL FILLI(BESTP,MAXMOT,0) + CALL FILLC(BESTQ,MAXMOT,0) + CALL FILLC(BESTS,MAXMOT,0.) + ITOTAL = 0 + CALL SETCMP(EXPECC,4) + PROMPT(1) = 'Motif by motif' + PROMPT(2) = 'Inclusive' + PROMPT(3) = 'Scores only' + PROMPT(4) = 'Complete padded sequences' + PROMPT(5) = 'Padded sections' + IOPT = 1 + CALL RADION('Select results display mode',PROMPT,5,IOPT, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOPT.LT.1) RETURN + IF(IOPT.EQ.4)THEN + MININ = 1 + MAXIN = 9999 + JSTART = 1 + WRITE(KBOUT,1020) +1020 FORMAT( + +' For output option 4, we need to position the first motifs',/, + +' in a pattern so that they are aligned with one another') + CALL GETINT(MININ,MAXIN,JSTART,'Position of first motif', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + JSTART = IVAL + END IF + JOPT = 0 + CALL YESNO(JOPT,'Report all matches', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(JOPT.LT.0) RETURN + FILNAM = ' ' + CALL OPENF1(IDEV3,FILNAM,0,IOK,KBIN,KBOUT, + + 'Pattern definition file', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + JDEV = IDEV3 +C +C GET MOTIF DEFINITIONS +C +C RETURN STRING LENGTH FOR COMPATIBILITY WITH LIB SEARCH + NSTRNG = MAXSTR + CALL GETMF(KBIN,KBOUT,STRING,NSTRNG,ISTRNG, + + LENGTH,MAXMOT,CLASS,RELMOT,RANGES,RANGEL, + + RANGET,RANGEM, + + STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF,IDEV2, + + WTSTR,JDEV,IOK,RELEND,IDSEQ,IDEV4,IDM,COMBIN, + + MAXCLS, + + MAT1,IDM,MAT2,IDME,MAT3,IDME,MAT4,IDM, + + PMINT,PMAXT,PROBT,EXPTT, + + IHELPS,IHELPE,HELPF,IDEVH,KEYNS,NAMSAV,FILNAM,0,TITLEP) + IF(IOK.NE.0)RETURN + IF(NMOT.LT.1)RETURN +C +C +C DISPLAY THE SIGNAL DESCRIPTION +C + RANGES(1) = 1 + CALL DESSIG( + + KBOUT,STRING,MAXSTR, + + LENGTH,CLASS,RELMOT,RANGES,RANGEL, + + RANGET,RANGEM, + + STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF, + + WTSTR,RELEND,COMBIN,KEYNS,TITLEP) + WRITE(KBOUT,2003)PROBT +2003 FORMAT(' Probability of finding pattern = ',E10.4) + WRITE(KBOUT,2004)EXPTT +2004 FORMAT(' Expected number of matches per 1000 residues = ',E10.4) + CALL GETRL(0.,1.,1.,'Maximum pattern probability', + +XP,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + PMINC = XP + CALL GETRL(-9999.,9999.,-9999.,'Minimum pattern score', + +XP,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CUTSCR = XP + MINSCR = 9999999. + MAXSCR = -9999999. + IF(NSTRNG.GT.0)CALL CONNUM(STRING,STRNGI,NSTRNG) +C +C + ICREC = 0 + IFINEX = 0 + NENTRY = 0 + ENTRYN = 0 +20 CONTINUE + IDSEQ = MAXSEQ + IF(LIBTYP.EQ.1) THEN + CALL CDROML(LIST,NAMIN,ENAMEL, + + IDEVEN,RSIZEN,NRECEN,IDEVNL,SEQ,IDSEQ, + + DIVDEV,NDIV,ICREC,IFINEX,TITLE,KBOUT,LTYPE,IOK) + IF(IOK.NE.0) GO TO 900 + FILNAM = NAMIN + ELSE IF(LIBTYP.EQ.2) THEN + CALL RDPIRA(SEQ,IDSEQ, + + IDEVEN,KBOUT,TITLE,FILNAM,LIST,ENAMEL,IDEVNL) + ELSE IF(LIBTYP.EQ.3) THEN + CALL RDFASA(SEQ,IDSEQ, + + IDEVEN,KBOUT,TITLE,FILNAM,LIST,ENAMEL,IDEVNL,ENTRYN) + ELSE + RETURN + END IF + IF(IDSEQ.LT.1)GO TO 900 + NENTRY = NENTRY + 1 +C CONVERT TO INTEGER + IF(IDSEQ.GT.0)CALL CONNUM(SEQ,SEQI,IDSEQ) +C + RANGES(1) = 1 + RANGEL(1) = IDSEQ +C +C DO THE SEARCH +C + CALL SRCSIH(KBIN,KBOUT,WTSTR,LENGTH,CLASS, + +RANGES,RANGEL,START,IEND,RELMOT,MATCHP,STRNGS,WEIGHT, + +MAXWTS,CUTOFF,MATCHS,NMOT,STRING,MAXSTR,SEQ,IDSEQ, + +RANGET,RANGEM,IENTRY,START2,IEND2,MATCHQ,RELEND, + +IDEV1,LAST5,LAST3S,LAST3E,IOPT, + +ITOTAL,KSTART,IDM,COMBIN, + +CUTSCR,MINSCR,MAXSCR,IDME, + +SEQI,STRNGI,TITLE,FILNAM, + +PMINT,PMAXT,PROBT,MAT1,MAT2,MAT3, + +MAT4,PMINC,IDEVN,KEYNS,BESTP,BESTQ,BESTS,JOPT) +C +C + GO TO 20 +C +C +900 CONTINUE + WRITE(KBOUT,1002)ITOTAL +1002 FORMAT(' Total matches found',I7) + WRITE(KBOUT,1006)MINSCR,MAXSCR +1006 FORMAT(' Minimum and maximum observed scores=',2F12.2) + WRITE(KBOUT,1009)NENTRY +1009 FORMAT(' ',I7,' Entries processed') + END + SUBROUTINE SRCSIH(KBIN,KBOUT,WTSTR,LENGTH,CLASS, + +RANGES,RANGEL,START,IEND,RELMOT,MATCHP,STRNGS,WEIGHT, + +MAXWTS,CUTOFF,MATCHS,NMOT,STRING,MAXSTR,SEQ,IDSEQ, + +RANGET,RANGEM,IENTRY,START2,IEND2,MATCHQ,RELEND, + +IDEVOT,LAST5,LAST3S,LAST3E, + +IOPT,ITOTAL,KSTART,IDM,COMBIN, + +CUTSCR,MINSCR,MAXSCR,IDME,SEQI,STRNGI,TITLE,FILNAM, + +PMINT,PMAXT,PROBT,MAT1,MAT2,MAT3, + +MAT4,PMINC,IDEVN,KEYNS,BESTP,BESTQ,BESTS,JOPT) +C ROUTINE TO SEARCH FOR SIGNALS COMPOSED OF MOTIFS +C WEIGHT = WEIGHTS FOR MATRICES +C CUTOFF = CUTOFF SCORES +C LENGTH = MOTIF LENGTHS +C CLASS = MOTIF CLASS +C COMBIN = LOGICAL COMBINATION A, O, N +C WTSTR = POINTER TO WEIGHT STARTS +C RANGES = RANGES START +C RANGEL = RANGE LENGTH (A DISTANCE MEASURED FROM RANGES) +C RELMOT = MOTIF NUMBER THAT A RANGE IS RELATIVE TO IE THE +C FIRST MOTIF'S RANGE IS RELATIVE TO MOTIF 0, BUT +C ANY OTHER MOTIF MAY HAVE TO BE DEFINED RELATIVE +C TO ANY OTHER. THE MOST COMMON WOULD BE THE FIRST +C MOTIF OR THE LAST ONE SEARCHED FOR. +C RELEND = IS A SPECIAL CASE FLAG FOR STEMS. IT ALLOWS OTHER +C MOTIFS TO HAVE THEIR POSITIONS RELATIVE TO THE 3' SIDE +C OF A STEM. IT IS 5 FOR THE 5 PRIME SIDE, 3 FOR 3' SIDE +C START = RANGE START DURING SEARCH (SOME POSITIONS MAY HAVE +C BEEN TRIED) +C IEND = RANGE END POSITION FOR CURRENT INITIAL START (WHEN IT +C IS RESET DEPENDS ON WHETHER IT IS DEFINED RELATIVE TO +C THE FIRST OR THE PREVIOUS MOTIF. IF IT IS DEFINED RELATIVE +C TO THE FIRST MOTIF IT IS RESET WHEN WE FIND A MATCH FOR THE +C FIRST MOTIF. IF IT IS DEFINED RELATIVE TO THE PREVIOUS MOTIF +C WE MUST RESET WHEN WE MOVE FORWARD ONE MOTIF. I THINK THIS +C CAN BE TAKEN CARE OF BY UPDATING ALL THOSE MOTIFS THAT ARE +C DEFINED TO THE CURRENT MOTIF EVERY TIME WE MOVE FORWARD +C ONE MOTIF (IE IT INCLUDES THE FIRST MOTIF SO IT IS NOT A +C SPECIAL CASE). OTHERWISE WE UPDATE POSITIONS WHEN WE FIND +C A MATCH FOR THEM (WE SET TO THE MATCH POSITION PLUS 1) +C MATCHP = LIST OF CURRENT MATCH POSITIONS FOR EACH MOTIF +C MATCHS = LIST OF CURRENT MATCH SCORES FOR EACH MOTIF +C IFOUND = A FLAG TO INDICATE SUCCESS OR FAILURE OF A SEARCH ROUTINE +C 1 = SUCCESS, 0 = FAIL +C STRNGS = POINTER TO STRING STARTS IN CHARACTER ARRAY STRING +C TEMPORARY VALUES ARE: +C MOTIF = ACTUAL MOTIF NUMBER +C ICLASS = CLASS +C ILEN = LENGTH OF MOTIF +C CUT = CUTOFF +C WT = START OF WEIGHTS FOR THIS MOTIF +C ISTRST = START OF STRING +C RANGET = START OF 3' RANGE FOR STEM SEARCHES +C RANGEM = END OF 3' RANGE FOR STEM SEARCHES +C IENTRY = FLAG TO SIGNIFY MORE 3' STEM POSITIONS FOR LAST 5' START +C 0 = NONE, ON RETURN FROM MOTIF6 IT CONTAINS THE 3' MATCH +C POSITION +C MATCHQ = MATCH POSITION FOR STEM SEARCH +C COMB = LOGICAL COMBINATION A, O, N + INTEGER WTSTR(NMOT),LENGTH(NMOT),CLASS(NMOT) + INTEGER RANGES(NMOT),RANGEL(NMOT),START(NMOT),IEND(NMOT) + INTEGER RELMOT(NMOT),MATCHP(NMOT),STRNGS(NMOT) + INTEGER RANGET(NMOT),RANGEM(NMOT),IENTRY(NMOT),RELEND(NMOT) + INTEGER START2(NMOT),IEND2(NMOT),MATCHQ(NMOT) + INTEGER LAST5(NMOT),LAST3S(NMOT),LAST3E(NMOT) + REAL WEIGHT(MAXWTS),CUTOFF(NMOT),MATCHS(NMOT) + REAL MINSCR,MAXSCR + CHARACTER SEQ(IDSEQ),STRING(MAXSTR) + CHARACTER COMBIN(NMOT),COMB + INTEGER STRNGI(MAXSTR),SEQI(IDSEQ) + CHARACTER TITLE*(*),FILNAM*(*) + CHARACTER*(*) KEYNS(NMOT) +C MAT1 SIMPLE IDENTITY +C MAT2 IUB SCORES 0-1 +C MAT3 IUB SCORES 0-36 +C MAT4 INVERTED REPEAT + INTEGER MAT1(IDM,IDM),MAT2(IDME,IDME) + INTEGER MAT3(IDME,IDME),MAT4(IDM,IDM) + INTEGER BESTP(NMOT),BESTQ(NMOT) + REAL BESTS(NMOT),MAXSS,MINMSS + PARAMETER (MINMSS = -999999.) +C +C +C INITIALIZE + JMOT = 0 + IRET = 0 +5 CONTINUE + JMOT = JMOT + 1 + IF(JMOT.LE.NMOT)THEN + IF(RELMOT(JMOT).EQ.0)THEN + START(JMOT) = RANGES(1) + IEND(JMOT) = RANGES(1) + RANGEL(1) -1 + GO TO 5 + END IF + END IF + MOTIF = 1 + ICLASS = CLASS(1) + ILEN = LENGTH(1) + CUT = CUTOFF(1) + IWT = WTSTR(1) + ISTRST = STRNGS(1) + IENTRY(1) = 0 + COMB = COMBIN(1) + DO 10 I = 1,NMOT + MATCHP(I) = 0 +10 CONTINUE + MAXSS = MINMSS +C +C +C +C +C +100 CONTINUE +C +C +C THIS A CLASS CLASS MOTIF, PERFORM THE APPROPRIATE SEARCH IF THE START +C POSITION IS >0. (IF IT IS NOT THE CURRENT MOTIF IS A NOT THAT HAS +C ALREADY BEEN SEARCHED FOR +C + IFOUND = 0 + IF(START(MOTIF).GT.0)THEN +C +C + IF(ICLASS.EQ.1)THEN + CALL MOTIF1(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF),IFOUND, + + CUTOFF(MOTIF),0) + ELSE IF(ICLASS.EQ.2)THEN + CALL MOTIF2(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),CUT,MATCHP(MOTIF),MATCHS(MOTIF),IFOUND) + ELSE IF(ICLASS.EQ.3)THEN + CALL MOTFI3(SEQI,IDSEQ,STRNGI(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),CUT,MATCHP(MOTIF),MATCHS(MOTIF),IFOUND,MAT3, + + IDME) + ELSE IF(ICLASS.EQ.4)THEN + CALL MOTFI4(SEQI,IDSEQ,ILEN,START(MOTIF), + + IEND(MOTIF),WEIGHT(IWT),CUT,MATCHP(MOTIF),MATCHS(MOTIF), + + IFOUND,IDM) + ELSE IF(ICLASS.EQ.5)THEN + CALL MOTFI4(SEQI,IDSEQ,ILEN,START(MOTIF), + + IEND(MOTIF),WEIGHT(IWT),CUT,MATCHP(MOTIF),MATCHS(MOTIF), + + IFOUND,IDM) + ELSE IF(ICLASS.EQ.6)THEN + CALL MOTIF6(SEQI,IDSEQ,MAT4,LENGTH(MOTIF),START(MOTIF), + + IEND(MOTIF),RANGET(MOTIF),RANGEM(MOTIF), + + CUTOFF(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF), + + IENTRY(MOTIF),IFOUND,MATCHQ(MOTIF), + + LAST5(MOTIF),LAST3S(MOTIF),LAST3E(MOTIF),IDM) + ELSE IF(ICLASS.EQ.7)THEN + CALL MOTIF1(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF),IFOUND, + + CUTOFF(MOTIF),1) + ELSE IF(ICLASS.EQ.8)THEN + CALL MOTIF8(SEQI,IDSEQ,MAT1,LENGTH(MOTIF),START(MOTIF), + + IEND(MOTIF),RANGET(MOTIF),RANGEM(MOTIF), + + CUTOFF(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF), + + IENTRY(MOTIF),IFOUND,MATCHQ(MOTIF), + + LAST5(MOTIF),LAST3S(MOTIF),LAST3E(MOTIF),IDM) + ELSE + WRITE(KBOUT,*)'UNKNOWN CLASS!!' + END IF +C +C + END IF +C +C +C MATCH FOUND WHEN MATCH WANTED ? +C +C +C + IF(((IFOUND.EQ.0).AND.(COMB.NE.'N')).OR. + + ((IFOUND.GT.0).AND.(COMB.EQ.'N')))THEN +C +C NO SO GO BACK OR SIDEWAYS ONE MOTIF +C +C + CALL BAKSID(CLASS,LENGTH,CUTOFF,STRNGS,NMOT, + + MOTIF,ICLASS,ILEN,CUT,IWT,ISTRST,WTSTR, + + RELMOT,START,IEND,MATCHQ,RANGES,RANGEL,RELEND,IRET,MATCHP, + + COMBIN,COMB) +C +C +C IF CANT GO BACK ANY FURTHER QUIT + IF(IRET.NE.0) THEN + IF(MAXSS.GT.MINMSS) THEN + CALL DSPLAZ(MATCHP,LENGTH,NMOT,SEQ,IDSEQ,IDEVOT, + + CLASS,MATCHQ,IOPT,KSTART,MATCHS,CUTSCR,MINSCR,MAXSCR, + + TITLE,FILNAM, + + PMINT,PMAXT,PROBT,IDM,MAT1,IDM,MAT2,IDME,MAT3,IDM, + + MAT4,IDM,WEIGHT,MAXWTS,WTSTR,CUTOFF,PMINC,RANGES,RANGEL, + + IDEVN,KEYNS,BESTP,BESTQ,BESTS,2,MAXSS) + ITOTAL = ITOTAL + 1 + END IF + RETURN + END IF +C +C + ELSE +C +C +C MATCH FOUND. +C +C +C PREPARE FOR NEXT SEARCH THIS MOTIF BY INCREMENTING POINTER +C TO SEARCH RANGE (NOT FOR CLASS 6 WHICH IS HANDLED BY MOTIF6) +C +C + IF(COMB.EQ.'N')THEN + START(MOTIF) = -9 + ELSE + IF((ICLASS.NE.6).AND.(ICLASS.NE.8)) + + START(MOTIF) = MATCHP(MOTIF) + 1 + IF(ICLASS.EQ.7)START(MOTIF) = MATCHP(MOTIF) + CUTOFF(MOTIF) + END IF +C +C TRY GOING FORWARD ONE MOTIF +C +C + CALL FORWAD(CLASS,LENGTH,CUTOFF,NMOT, + + MOTIF,ICLASS,ILEN,CUT,IWT,RELMOT,START,IEND, + + RANGES,RANGEL,STRNGS,ISTRST,WTSTR,IDSEQ,IENTRY, + + RANGET,RANGEM,START2,IEND2,MATCHQ,RELEND,MATCHP,IDSPLY, + + COMBIN,COMB) +C +C +C +C IS THIS THE LAST MOTIF? IF SO DISPLAY THE MATCH +C +C + IF(IDSPLY.EQ.1)THEN + IF(JOPT.NE.1) ITOTAL = ITOTAL + 1 + CALL DSPLAZ(MATCHP,LENGTH,NMOT,SEQ,IDSEQ,IDEVOT, + + CLASS,MATCHQ,IOPT,KSTART,MATCHS,CUTSCR,MINSCR,MAXSCR, + + TITLE,FILNAM, + + PMINT,PMAXT,PROBT,IDM,MAT1,IDM,MAT2,IDME,MAT3,IDM, + + MAT4,IDM,WEIGHT,MAXWTS,WTSTR,CUTOFF,PMINC,RANGES,RANGEL, + + IDEVN,KEYNS,BESTP,BESTQ,BESTS,JOPT,MAXSS) +C +C +C HORRIBLE SPECIAL CASE - IF LAST MOTIF IS NOTTED WE MUST MOVE BACK AFTER +C DISPLAY + IF(COMB.EQ.'N')THEN + CALL BAKSID(CLASS,LENGTH,CUTOFF,STRNGS,NMOT, + + MOTIF,ICLASS,ILEN,CUT,IWT,ISTRST,WTSTR, + + RELMOT,START,IEND,MATCHQ,RANGES,RANGEL,RELEND,IRET,MATCHP, + + COMBIN,COMB) +C +C +C IF CANT GO BACK ANY FURTHER QUIT + IF(IRET.NE.0) THEN + IF(MAXSS.GT.MINMSS) THEN + CALL DSPLAZ(MATCHP,LENGTH,NMOT,SEQ,IDSEQ,IDEVOT, + + CLASS,MATCHQ,IOPT,KSTART,MATCHS,CUTSCR,MINSCR,MAXSCR, + + TITLE,FILNAM, + + PMINT,PMAXT,PROBT,IDM,MAT1,IDM,MAT2,IDME,MAT3,IDM, + + MAT4,IDM,WEIGHT,MAXWTS,WTSTR,CUTOFF,PMINC,RANGES,RANGEL, + + IDEVN,KEYNS,BESTP,BESTQ,BESTS,2,MAXSS) + ITOTAL = ITOTAL + 1 + END IF + RETURN + END IF + END IF + END IF +C +C + END IF +C +C +C GO BACK FOR NEXT SEARCH +C +C + GO TO 100 + END +C********************************************************************* + SUBROUTINE MOTIF8(SEQ,IDSEQ,MATRIX,LENGTH,I5STAR,I5END, + + I3STAR,I3END,CUTOFF,MATCHP,MATCHS, + + IENTRY,IFOUND,MATCHQ, + + LAST5,LAST3S,LAST3E,IDM) +C AUTHOR: RODGER STADEN + INTEGER SEQ(IDSEQ) + INTEGER MATRIX(IDM,IDM),REPEET + REAL MATCHS + EXTERNAL REPEET +C WE HAVE A START POSITION FOR THE 5' END OF THE 5' END OF +C A POTENTIAL REPEAT I5STAR AND AN END DEFINED BY A RANGE I5END +C WE HAVE A REPEAT LENGTH LENGTH +C WE HAVE A RANGE OF POSITIONS FOR THE 3' STEM TO START +C I3STAR TO I3END +C TRY THE TIGHTEST LOOPS FIRST +C BUT FIRST WE MAY HAVE TO FINISH A PREVIOUS SEARCH +C THIS IS DENOTED BY IENTRY NE 0. +C NOTE IENTRY IS ALSO USED TO RETURN THE 3' MATCH POSITION + ICUT = CUTOFF +C WRITE(*,*)'ICUT',ICUT + IFOUND = 0 + JENTRY = IENTRY + IENTRY = 0 + IF(I5STAR.LT.1)I5STAR=1 + IF((I5STAR+I3STAR+LENGTH-2).GT.IDSEQ)RETURN + IF(JENTRY.NE.0)THEN + I1 = LAST5 +C WRITE(*,*)'I1,LAST3S,LAST3E',I1,LAST3S,LAST3E + DO 50 J=LAST3S+1,LAST3E + J1 = J + ISUM = REPEET(SEQ,IDSEQ,MATRIX,LENGTH,I1,J1,IDM) +C RETURN IF GOOD ENOUGH + IF(ISUM.GE.ICUT)THEN + MATCHP = I1 + IENTRY = J1 + MATCHQ = J1 + MATCHS = ISUM + IFOUND = MATCHP + LAST3S = J1 + RETURN + END IF +50 CONTINUE +C NOW MOVE 5' STEM START POSITION (WE HAVE JUST FINISHED THE LAST) +C TO THE LAST MATCH + 1 + I5STAR = MATCHP + 1 + END IF +C SET ENTRY FLAG TO ZERO TO SIGNIFY LAST SEARCH NOW COMPLETED + IENTRY = 0 + ISUM = 0 + LOOPI1 = I5STAR + IF((I5STAR+I3STAR+LENGTH-2).GT.IDSEQ)RETURN + LOOPI2 = MIN(IDSEQ-2*LENGTH+1,I5END) +C WRITE(*,*)'IDSEQ,LENGTH,I5STAR,I5END', +C +IDSEQ,LENGTH,I5STAR,I5END +C +C TRY ALL STEM STARTS FROM 5' START TO 5' END +C +C + DO 200 I = LOOPI1,LOOPI2 +C +C + I1 = I +C +C TRY ALL LOOPS FROM 3' START TO 3' END +C + LOOPJ1 = I + I3STAR -1 + IF((LOOPJ1+LENGTH-1).GT.IDSEQ)RETURN + LOOPJ2 = MIN(IDSEQ-LENGTH+1,I+I3END-1) +C WRITE(*,*)'I3STAR,I3END',I3STAR,I3END +C +C +C + DO 100 J = LOOPJ1,LOOPJ2 +C +C +C + J1 = J +C IN REPEAT NOTE THAT +C THE 5' END POINTER I1 GOES FORWARDS +C THE 3' END POINTER J1 GOES FORWARDS +C + ISUM = REPEET(SEQ,IDSEQ,MATRIX,LENGTH,I1,J1,IDM) +C RETURN IF GOOD ENOUGH +C WRITE(*,*)ISUM + IF(ISUM.GE.ICUT)THEN + MATCHP = I1 + IENTRY = J1 + MATCHQ = J1 + MATCHS = ISUM + IFOUND = MATCHP +C SAVE CURRENT POSITION FOR LATER ENTRIES + LAST5 = I1 + LAST3S = J1 + LAST3E = LOOPJ2 + RETURN + END IF +100 CONTINUE +200 CONTINUE + END +C********************************************************************* + INTEGER FUNCTION REPEET(SEQ,IDSEQ,MATRIX,LENGTH,I5P,I3P,IDM) + INTEGER SEQ(IDSEQ) + INTEGER MATRIX(IDM,IDM) +C THE 5' END POINTER GOES FORWARDS +C THE 3' END POINTER GOES FORWARDS + L=0 + I5=I5P-1 + I3=I3P-1 + DO 100 I=1,LENGTH + I5 = I5 + 1 + I3 = I3 + 1 +C WRITE(*,*)'I5,I3',I5,I3 + L5 = SEQ(I5) + L3 = SEQ(I3) + L = L + MATRIX(L5,L3) +100 CONTINUE + REPEET = L + END + SUBROUTINE PADOUT(IDEV,CHAR,LINLEN,NCHAR) + CHARACTER CHAR +C HOW MANY LINES? + NLINE = 1 + (NCHAR-1)/LINLEN + K1 = 1 + K2 = MIN(NCHAR,LINLEN) + DO 10 I = 1,NLINE + WRITE(IDEV,1000,ERR=20)(CHAR,K=K1,K2) + K1 = K2 + 1 + K2 = K1 + LINLEN - 1 + K2 = MIN(K2,NCHAR) +10 CONTINUE +20 CONTINUE +1000 FORMAT(' ',60A1) + END +C********************************************************************* + SUBROUTINE MOTFI3(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND,CUTOFF, + +MATCHP,MATCHS,IFOUND,MATRIX,IDM) + INTEGER SEQ(IDIM1),STRING(IDIM2) + INTEGER MATRIX(IDM,IDM) + REAL MATCHS + IFOUND = 0 + IF(ISTART.LT.1)ISTART=1 + IF(ISTART.GT.IDIM1)RETURN + CALL SQFTI5(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND,CUTOFF,MATCHS, + +IFOUND,MATRIX,IDM) + IF(IFOUND.EQ.0)RETURN +C SAVE MATCH POSITION + MATCHP = IFOUND + RETURN + END +C********************************************************************* + SUBROUTINE SQFTI5(SEQ,IDIM1,STRING,IDIM2, + 1IS,IE,MINSC,MATCHS,IFOUND,MATRIX,IDM) +C AUTHOR: RODGER STADEN + INTEGER SEQ(IDIM1),STRING(IDIM2) + REAL MATCHS,MINSC + INTEGER MATRIX(IDM,IDM) + MINSCR = MINSC +C + IDIF=(IE-IS+2)-IDIM2 +C IDIF IS THE NUMBER OF POSNS TO TRY +C IPSTR GOES FROM 1 TO IDIM2 IDIF TIMES +C TRY ALL POSSIBLE POSITIONS FOR MATCHING AND SCORE FOR EACH +C POINT TO ARRAY ELEMENT CORRESPONDING TO FIRST BASE + IPSEQ=IS + DO 200 I=1,IDIF + NTOT=0 + IP=IPSEQ + DO 100 J=1,IDIM2 + NTOT = NTOT + MATRIX(SEQ(IP),STRING(J)) + IP=IP+1 +100 CONTINUE +C END OF COUNTING FOR THIS POSITION.IS TOTAL HIGH ENOUGH? + IF(NTOT.GE.MINSCR)THEN + MATCHS = NTOT + IFOUND = IP-IDIM2 + RETURN + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + IFOUND = 0 + RETURN + END +C********************************************************************* + SUBROUTINE MOTFI4(SEQ,IDIM1,LENGTH,ISTART,IEND, + +WEIGHT,CUTOFF,MATCHP,MATCHS,IFOUND,IDM) + REAL WEIGHT(IDM,LENGTH) + REAL MATCHS + INTEGER SEQ(IDIM1) + IFOUND = 0 + IF(ISTART.LT.1)ISTART=1 + L1 = IEND-ISTART+1 + IF(ISTART.GT.IDIM1)RETURN + IF(L1.LT.LENGTH)RETURN + DO 10 I=ISTART,ISTART+L1-LENGTH + SUM = 0. + K = 0 + DO 5 J=I,I+LENGTH-1 + K = K + 1 + SUM = SUM + WEIGHT(SEQ(J),K) + 5 CONTINUE + IF(SUM.GE.CUTOFF) THEN + MATCHP = I + MATCHS = SUM + IFOUND = I + RETURN + END IF + 10 CONTINUE + IFOUND = 0 + END +C********************************************************************* + SUBROUTINE FMOTI4(SEQ,IDIM,WT,LENGTH,CUTOFF,SUM,IFOUND,IDM) +C AUTHOR: RODGER STADEN + INTEGER SEQ(IDIM) + REAL WT(IDM,LENGTH) + DO 10 I=1,IDIM-LENGTH+1 + SUM=0. + K=0 + DO 5 J=I,I+LENGTH-1 + K=K+1 + SUM=SUM+WT(SEQ(J),K) +5 CONTINUE + IF(SUM.GE.CUTOFF)THEN + IFOUND = I + RETURN + END IF +10 CONTINUE + IFOUND = 0 + RETURN + END +C********************************************************************* + SUBROUTINE MOTIF6(SEQ,IDSEQ,MATRIX,LENGTH,I5STAR,I5END, + + I3STAR,I3END,CUTOFF,MATCHP,MATCHS, + + IENTRY,IFOUND,MATCHQ, + + LAST5,LAST3S,LAST3E,IDM) +C AUTHOR: RODGER STADEN + INTEGER SEQ(IDSEQ) + INTEGER MATRIX(IDM,IDM) + REAL MATCHS + EXTERNAL LOOP +C WE HAVE A START POSITION FOR THE 5' END OF THE 5' END OF +C A POTENTIAL STEM I5STAR AND AN END DEFINED BY A RANGE I5END +C WE HAVE A STEM LENGTH LENGTH +C WE HAVE A RANGE OF POSITIONS FOR THE 3' STEM TO START +C I3STAR TO I3END +C TRY THE TIGHTEST LOOPS FIRST +C BUT FIRST WE MAY HAVE TO FINISH A PREVIOUS SEARCH +C THIS IS DENOTED BY IENTRY NE 0. +C NOTE IENTRY IS ALSO USED TO RETURN THE 3' MATCH POSITION + ICUT = CUTOFF +C WRITE(*,*)'ICUT',ICUT + IFOUND = 0 + JENTRY = IENTRY + IENTRY = 0 + IF(I5STAR.LT.1)I5STAR=1 + IF((I5STAR+I3STAR-1).GT.IDSEQ)RETURN + IF(JENTRY.NE.0)THEN + I1 = LAST5 +C WRITE(*,*)'I1,LAST3S,LAST3E',I1,LAST3S,LAST3E + DO 50 J=LAST3S+1,LAST3E + J1 = J + ISUM = LOOP(SEQ,IDSEQ,MATRIX,LENGTH,I1,J1,IDM) +C RETURN IF GOOD ENOUGH + IF(ISUM.GE.CUTOFF)THEN + MATCHP = I1 + IENTRY = J1 + MATCHQ = J1 + MATCHS = ISUM + IFOUND = MATCHP + LAST3S = J1 + RETURN + END IF +50 CONTINUE +C NOW MOVE 5' STEM START POSITION (WE HAVE JUST FINISHED THE LAST) +C TO THE LAST MATCH + 1 + I5STAR = MATCHP + 1 + END IF +C SET ENTRY FLAG TO ZERO TO SIGNIFY LAST SEARCH NOW COMPLETED + IENTRY = 0 + ISUM = 0 + LOOPI1 = I5STAR + IF((I5STAR+I3STAR-1).GT.IDSEQ)RETURN + LOOPI2 = MIN(IDSEQ,I5END) +C WRITE(*,*)'IDSEQ,LENGTH,I5STAR,I5END', +C +IDSEQ,LENGTH,I5STAR,I5END +C +C TRY ALL STEM STARTS FROM 5' START TO 5' END +C +C + DO 200 I = LOOPI1,LOOPI2 +C +C + I1 = I +C +C TRY ALL LOOPS FROM 3' START TO 3' END +C + LOOPJ1 = I+I3STAR-1 + IF(LOOPJ1.GT.IDSEQ)RETURN + LOOPJ2 = MIN(IDSEQ,I+I3END-1) +C WRITE(*,*)'I3STAR,I3END',I3STAR,I3END +C +C +C + DO 100 J = LOOPJ1,LOOPJ2 +C +C +C + J1 = J +C IN LOOP NOTE THAT +C THE 5' END POINTER I1 GOES FORWARDS +C THE 3' END POINTER J1 GOES BACKWARDS +C + ISUM = LOOP(SEQ,IDSEQ,MATRIX,LENGTH,I1,J1,IDM) +C RETURN IF GOOD ENOUGH +C WRITE(*,*)ISUM + IF(ISUM.GE.ICUT)THEN + MATCHP = I1 + IENTRY = J1 + MATCHQ = J1 + MATCHS = ISUM + IFOUND = MATCHP +C SAVE CURRENT POSITION FOR LATER ENTRIES + LAST5 = I1 + LAST3S = J1 + LAST3E = LOOPJ2 + RETURN + END IF +100 CONTINUE +200 CONTINUE + END +C********************************************************************* + INTEGER FUNCTION LOOP(SEQ,IDSEQ,MATRIX,LENGTH,I5P,I3P,IDM) + INTEGER SEQ(IDSEQ) + INTEGER MATRIX(IDM,IDM) +C THE 5' END POINTER GOES FORWARDS +C THE 3' END POINTER GOES BACKWARDS + L=0 + I5=I5P-1 + I3=I3P+1 + DO 100 I=1,LENGTH + I5 = I5 + 1 + I3 = I3 - 1 + L5 = SEQ(I5) + L3 = SEQ(I3) + L = L + MATRIX(L5,L3) +100 CONTINUE + LOOP = L + END + SUBROUTINE SETCMP(COMPIN,IDM) + REAL COMPIN(IDM) + PARAMETER (MAXCHR = 17) + COMMON /COMPC/COMP(MAXCHR) + SAVE /COMPC/ + DO 10 I = 1,MAXCHR + COMP(I) = 0.0 +10 CONTINUE + T = 0. + DO 20 I = 1,IDM + COMP(I) = COMPIN(I) + T = T + COMPIN(I) +20 CONTINUE + DO 30 I = 1,IDM + COMP(I) = COMP(I) / T +30 CONTINUE + END +C***************************************************************** + SUBROUTINE DSPLAZ(MATCHP,LENGTH,NMOT,SEQ,IDSEQ,IDEV, + +CLASS,MATCHQ,IOPT,KSTART,MATCHS,CUTSCR,MINSCR,MAXSCR, + +TITLE,FILNAM, + + PMINT,PMAXT,PROBT,IDM,MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3, + + MAT4,IDMAT4,WEIGHT,MAXWTS,WTSTR,CUTOFF,PMINC, + + RANGES,RANGEL,IDEV1,KEYNS,BESTP,BESTQ,BESTS,JOPT,MAXSS) + INTEGER RANGES(NMOT),RANGEL(NMOT) + INTEGER MATCHP(NMOT),LENGTH(NMOT),CLASS(NMOT) + INTEGER MATCHQ(NMOT) + CHARACTER SEQ(IDSEQ),TITLE*(*),FILNAM*(*) + CHARACTER*(*) KEYNS(NMOT) + REAL MATCHS(NMOT),MINSCR,MAXSCR + INTEGER WTSTR(NMOT) + REAL WEIGHT(MAXWTS),CUTOFF(NMOT) +C MAT1 SIMPLE IDENTITY +C MAT2 IUB SCORES 0-1 +C MAT3 IUB SCORES 0-36 +C MAT4 INVERTED REPEAT + INTEGER MAT1(IDMAT1,IDMAT1),MAT2(IDMAT2,IDMAT2) + INTEGER MAT3(IDMAT3,IDMAT3),MAT4(IDMAT4,IDMAT4) + CHARACTER DASH +C stuff for best + INTEGER BESTP(NMOT),BESTQ(NMOT) + REAL BESTS(NMOT),MAXSS + EXTERNAL PSCORE + SAVE DASH + DATA DASH/'-'/ +C jopt 1 get best match for any individual sequence, then +C display it. So check each match for being best (>maxss), if it is +C save its coords in bestp, bestq. Keep a note that a score +C has been recorded for this sequence (actually noted by bestp(1) ne.0) +C when we finish a sequence (denoted by jopt = 2) put all the +C saved values into matchp, matchq and process as normal. + IF(JOPT.NE.0) THEN + IF(JOPT.EQ.2) THEN + DO 5 I=1,NMOT + MATCHP(I) = BESTP(I) + MATCHQ(I) = BESTQ(I) + MATCHS(I) = BESTS(I) + 5 CONTINUE + ELSE IF(JOPT.EQ.1) THEN +C Add scores + T = 0. + DO 6 I = 1,NMOT + IF(MATCHP(I).NE.0) T = T + MATCHS(I) + 6 CONTINUE + IF(T.GT.MAXSS) THEN + DO 7 I=1,NMOT + BESTP(I) = MATCHP(I) + BESTQ(I) = MATCHQ(I) + BESTS(I) = MATCHS(I) + 7 CONTINUE + MAXSS = T + END IF + RETURN + END IF + END IF +C Add scores + T = 0. + DO 10 I = 1,NMOT + IF(MATCHP(I).NE.0) T = T + MATCHS(I) +10 CONTINUE +C +C If a probability cut off set calc its value +C + POBS = 1.0 + IF(PMINC.LT.1.0)THEN + DO 20 I = 1,NMOT + IF(MATCHP(I).NE.0)THEN + CALL GETP(CLASS(I),SEQ(MATCHP(I)+KSTART-1),LENGTH(I), + + IDM,MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + + WEIGHT(MAX(1,WTSTR(I)))) + PROB = PSCORE(MATCHS(I)) + POBS = POBS * PROB + END IF +20 CONTINUE + IF((PMINC.LT.1.0).AND.(POBS.GT.PMINC))RETURN + END IF +C + IF(T.GT.MAXSCR) MAXSCR = T + IF(T.LT.MINSCR) MINSCR = T + IF(T.LT.CUTSCR) RETURN +C +C Write out mofif by motif +C + IF(IOPT.EQ.1)THEN + WRITE(IDEV,1001)FILNAM(1:10),T,TITLE +1001 FORMAT(' >',A,' ',F10.3,' ',A) + DO 100 I=1,NMOT + J = I +C Check for no match (needed for ored motifs) + IF(MATCHP(J).NE.0)THEN + WRITE(IDEV,1000)MATCHP(J)+KSTART-1,MATCHS(J),KEYNS(I) + WRITE(IDEV,1002)(SEQ(K),K=MATCHP(J),MATCHP(J)+LENGTH(J)-1) +C Repeat ? + IF(CLASS(J).EQ.8)THEN + WRITE(IDEV,1002)(SEQ(K),K=MATCHQ(J),MATCHQ(J)+LENGTH(J)-1) + WRITE(IDEV,1000)MATCHQ(J)+KSTART-1 + END IF +C Stem ? + IF(CLASS(J).EQ.6)THEN + WRITE(IDEV,1002) + + (SEQ(K),K=MATCHQ(J),MATCHQ(J)-LENGTH(J)+1,-1) + WRITE(IDEV,1000)MATCHQ(J)+KSTART-1 + END IF + END IF +100 CONTINUE +1000 FORMAT(' ',I7,' ',F10.3,' ',A8) +1002 FORMAT(' ',60A1) + IF(PMINC.LT.1.0)WRITE(IDEV,1004)POBS +1004 FORMAT(' Probability =',E10.4) + RETURN + END IF +C +C write only name, score, title +C + IF(IOPT.EQ.3)THEN + WRITE(IDEV,1001)FILNAM(1:10),T,TITLE + IF(PMINC.LT.1.0)WRITE(IDEV,1004)POBS + END IF +C +C write name, score, title and the inclusive match +C + IF(IOPT.EQ.2)THEN + WRITE(IDEV,1001)FILNAM(1:10),T,TITLE + MINP = 999999 + MAXP = -999999 + DO 300 I = 1, NMOT + K = MATCHP(I) + IF(K.NE.0)THEN + IF(K.LT.MINP)MINP = K + K = K + LENGTH(I) - 1 +C Inverted repeat ? + IF(CLASS(I).EQ.6) K = MATCHQ(I) +C Repeat ? + IF(CLASS(I).EQ.8) K = MATCHQ(I) + LENGTH(I) - 1 + IF(K.GT.MAXP)MAXP = K + END IF +300 CONTINUE + WRITE(IDEV,1000)MINP+KSTART-1 + WRITE(IDEV,1002) + + ((SEQ(K1),K1=K2,MIN(K2+59,MAXP)),K2=MINP,MAXP,60) + IF(PMINC.LT.1.0)WRITE(IDEV,1004)POBS + RETURN + END IF +C +C write out whole seq to a new file +C + IF(IOPT.EQ.4)THEN + WRITE(IDEV,1001)FILNAM(1:10),T,TITLE + CALL OPENRS(IDEV1,FILNAM,IOK,LLL,1) + IF(IOK.NE.0)THEN + WRITE(IDEV,*)' Error opening sequence file' + RETURN + END IF +C Write up to and including first motif + J1 = MATCHP(1)+LENGTH(1)-1 + WRITE(IDEV1,1005,ERR=401)(SEQ(K),K=1,J1) + DO 400 I=2,NMOT + J = I +C Put dashes for next gap in now. what is max gap? + IMAXG = RANGES(J) - LENGTH(J-1) + RANGEL(J) - LENGTH(J) +C What is actual gap? + IG = MATCHP(J) - MATCHP(J-1) - LENGTH(J-1) +C Want to put in difference number of dashes + IDASH = IMAXG - IG + IF(IDASH.GT.0)CALL PADOUT(IDEV1,DASH,60,IDASH) +C Write up to and including next motif + J2 = MATCHP(J) + LENGTH(J) - 1 + WRITE(IDEV1,1005,ERR=401)(SEQ(K),K=J1+1,J2) + J1 = J2 +400 CONTINUE +C Write to end of sequence + WRITE(IDEV1,1005,ERR=401)(SEQ(K),K=J1+1,IDSEQ) +401 CONTINUE + CLOSE(UNIT=IDEV1) +1005 FORMAT(' ',60A1) + END IF +C +C write inclusive to new file +C + IF(IOPT.EQ.5)THEN + WRITE(IDEV,1001)FILNAM(1:10),T,TITLE + CALL OPENRS(IDEV1,FILNAM,IOK,LLL,1) + IF(IOK.NE.0)THEN + WRITE(IDEV,*)' Error opening sequence file' + RETURN + END IF +C Write first motif + J1 = MATCHP(1)+LENGTH(1)-1 + WRITE(IDEV1,1005,ERR=501)(SEQ(K),K=MATCHP(1),J1) + DO 500 I=2,NMOT + J = I +C Put dashes for next gap in now. what is max gap? + IMAXG = RANGES(J) - LENGTH(J-1) + RANGEL(J) - LENGTH(J) +C What is actual gap? + IG = MATCHP(J) - MATCHP(J-1) - LENGTH(J-1) +C Want to put in difference number of dashes + IDASH = IMAXG - IG + IF(IDASH.GT.0)CALL PADOUT(IDEV1,DASH,60,IDASH) +C Write up to and including next motif + J2 = MATCHP(J) + LENGTH(J) - 1 + WRITE(IDEV1,1005,ERR=501)(SEQ(K),K=J1+1,J2) + J1 = J2 +500 CONTINUE +501 CONTINUE + CLOSE(UNIT=IDEV1) + END IF + END diff --git a/src/staden/nipmen.f b/src/staden/nipmen.f new file mode 100644 index 0000000..24e98d8 --- /dev/null +++ b/src/staden/nipmen.f @@ -0,0 +1,151 @@ +C nipmen +C 9-7-90 Reorganised lists + SUBROUTINE MENU(OPT,KOPT,MOPT,MAXOPT,MINMEN,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + INTEGER IHELPS(0:MAXOPT),IHELPE(0:MAXOPT) + INTEGER OPT + CHARACTER HELPF*(*) +1 CONTINUE + CALL CLEARV + IF(MOPT.EQ.0)THEN + WRITE(KBOUT,5000) +5000 FORMAT(' Menus and their numbers are '/ + +' m0 = This menu'/ + +' m1 = General'/ + +' m2 = Screen control'/ + +' m3 = Statistical analysis of content'/ + +' m4 = Structures and repeats'/ + +' m5 = Translation and codons'/ + +' m6 = Gene search by content'/ + +' m7 = General signals'/ + +' m8 = Specific signals'/ + +' ? = Help'/ + +' ! = Quit') +1001 FORMAT( + +' ? Menu or option number=',$) + ELSE IF(MOPT.EQ.-1)THEN + WRITE(KBOUT,5001) +5001 FORMAT(' General menu'/ + + ' 0 = List of menus'/ + + ' ? = Help'/ + + ' ! = Quit'/ + 1 ' 3 = Read a new sequence'/ + 1 ' 4 = Define active region'/ + + ' 5 = List the sequence'/ + + ' 6 = List a text file'/ + + ' 7 = Direct output to disk'/ + + ' 8 = Write active sequence to disk'/ + + ' 9 = Edit the sequence'/ + + ' 17 = Search for restriction enzymes'/ + + ' 29 = Reverse and complement the active sequence'/ + + ' 66 = Exchange t and u') + ELSE IF(MOPT.EQ.-2)THEN + WRITE(KBOUT,5002) +5002 FORMAT(' Screen control menu'/ + + ' 0 = List of menus'/ + + ' 10 = Clear graphics'/ + + ' 11 = Clear text'/ + + ' 12 = Draw a ruler'/ + + ' 13 = Use crosshair'/ + + ' 14 = Reposition plots'/ + + ' 15 = Label diagram'/ + + ' 16 = Display a map') + ELSE IF(MOPT.EQ.-3)THEN + WRITE(KBOUT,5005) +5005 FORMAT(' Statistical analysis of content'/ + + ' 0 = List of menus'/ + + ' 21 = Count bases'/ + + ' 22 = Count dinucleotides'/ + + ' 24 = Plot base composition'/ + 2 ' 25 = Plot local deviations in base composition'/ + 3 ' 26 = Plot local deviations in dinucleotide', + + ' composition'/ + 4 ' 27 = Plot local deviations in trinucleotide', + + ' composition'/ + 3 ' 38 = Examine repeats'/ + 5 ' 59 = Plot negentropy'/ + 6 ' 64 = Plot observed-expected word frequencies') + ELSE IF(MOPT.EQ.-4)THEN + WRITE(KBOUT,5007) +5007 FORMAT(' Structures and repeats menu'/ + + ' 0 = List of menus'/ + 5 ' 30 = Search for hairpin loops'/ + 1 ' 31 = Search for long range inverted repeats'/ + 1 ' 32 = Search for repeats'/ + 1 ' 33 = Search for z dna (total ry,yr)'/ + 1 ' 34 = Search for z dna (runs of ry or yr)'/ + 1 ' 35 = Search for z dna (best phased value)'/ + 1 ' 36 = Search for local similarity or complementarity'/ + 3 ' 38 = Examine repeats') + ELSE IF(MOPT.EQ.-5)THEN + WRITE(KBOUT,5006) +5006 FORMAT(' Translation and codon menu'/ + + ' 0 = List of menus'/ + + ' 23 = Count codons, amino acids and bases',/, + + ' 28 = Calculate codon constraint',/, + + ' 37 = Set genetic code'/ + 1 ' 39 = Translate and list in up to six phases'/ + 5 ' 40 = Translate and write protein sequence to disk'/ + 5 ' 41 = Write codon table to disk',/, + + ' 54 = Search for open reading frames') + ELSE IF(MOPT.EQ.-6)THEN + WRITE(KBOUT,5003) +5003 FORMAT(' Gene search by content'/ + + ' 0 = List of menus'/ + 1 ' 42 = Codon usage method'/ + 5 ' 43 = Positional base preferences method'/ + 5 ' 44 = Uneven positional base frequencies method'/ + 4 ' 45 = Codon improbability on base composition'/ + 4 ' 46 = Codon improbability on amino acid composition'/ + 2 ' 47 = Shepherd RNY preference method'/ + 3 ' 48 = Ficketts method'/ + 5 ' 49 = tRNA gene search') + ELSE IF(MOPT.EQ.-7)THEN + WRITE(KBOUT,5004) +5004 FORMAT(' General signals menu'/ + + ' 0 = List of menus'/ + + ' 18 = Compare a short sequence'/ + + ' 19 = Compare a short sequence using a score matrix'/ + + ' 20 = Search using a weight matrix'/ + 1 ' 50 = Plot start codons'/ + 2 ' 51 = Plot stop codons'/ + 3 ' 52 = Plot stop codons on the complementary strand'/ + 3 ' 53 = Plot stop codons on both strands'/ + + ' 60 = Search using a dinucleotide weight matrix'/ + + ' 63 = Search the complementary strand using a ', + + 'weight matrix'/ + + ' 67 = Search for patterns of motifs') + ELSE IF(MOPT.EQ.-8)THEN + WRITE(KBOUT,5008) +5008 FORMAT(' Eukaryotic signal search'/ + + ' 0 = List of menus'/ + 3 ' 55 = Search for E. coli promoters'/ + 3 ' 56 = Search for E. coli promoters', + + ' on the complementary strand'/ + 3 ' 57 = Search for E. coli promoters (-35 and -10)'/ + 4 ' 58 = Search for prokaryotic ribosome binding sites'/ + 5 ' 61 = Search for eukaryotic ribosome binding sites'/ + 6 ' 62 = Search for splice junctions'/ + 6 ' 65 = Search for polya sites') + END IF +3 CONTINUE + WRITE(KBOUT,1001) + CALL GETOPT(KBIN,KOPT,OPT) + IF((OPT.LT.MINMEN).OR.(OPT.GT.MAXOPT).OR.(KOPT.GT.2)) THEN + MOPT = 0 + GO TO 1 + END IF + IF((KOPT.EQ.2).AND.(OPT.GE.0))THEN + CALL HELP2(IHELPS(OPT),IHELPE(OPT),HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(OPT.EQ.0) THEN + MOPT = 0 + GO TO 1 + END IF + IF(OPT.LT.0) THEN + MOPT = OPT + GO TO 1 + END IF + END diff --git a/src/staden/nxhelpmenu.c b/src/staden/nxhelpmenu.c new file mode 100644 index 0000000..90a16b2 --- /dev/null +++ b/src/staden/nxhelpmenu.c @@ -0,0 +1,157 @@ +#include +#include +#include +#include +#include "helpnmenu.h" +#include "nxhelpmenu.h" +#include "userface.h" +#include "fort.h" + +static int linecount = 0; +static int currentmenu = 0; + +/* + * Returns 0 for ok, + * -1 for cancel. + */ +static int scroll(char *text) { + printf("%s", text); + linecount++; + + if (linecount > 20) { + linecount = 0; + return bpause(); + } + return 0; +} + +void menu_x(int_f *OPT_p, + int_f *KOPT_p, + int_f *MOPT_p, + int_f *MAXOPT_p, + int_f *MINMEN_p, + int_f *KBIN_p, + int_f *KBOUT_p, + int_f *IHELPS_p, + int_f *IHELPE_p, + char *HELPF_p, + int_f *IDEVH_p, + int_fl HELPF_l) +/* + * Display the menu dialogue and get the number of a function. + * OPT number of function selected + * KOPT=0 => OK + * KOPT=1 => dialogue wanted + */ +{ + int m, i, mnum; + menuarr menu[MAXOPTS]; + + memset((void *)menu, 0, MAXOPTS*sizeof(menuarr)); + /* should we ignore this arg totally!? */ + /* mnum = *MOPT_p; */ + mnum = currentmenu; + do { + m = create_menu(mnum, menu, MAXOPTS); + do { + puts(""); + if (mnum == 0) + puts(" Menus and their numbers are"); + else + printf(" %s menu\n", (helpindex - mnum)->name); + for (i=0; i0. (IF IT IS NOT THE CURRENT MOTIF IS A NOT THAT HAS +C ALREADY BEEN SEARCHED FOR +C + IFOUND = 0 + IF(START(MOTIF).GT.0)THEN +C +C + IF(ICLASS.EQ.1)THEN +C WRITE(*,*)'SRCSIG,MOTIF,ICLASS,START,END' +C WRITE(*,*)MOTIF,ICLASS,START(MOTIF),IEND(MOTIF) + CALL MOTIF1(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF),IFOUND, + + CUTOFF(MOTIF),0) + ELSE IF(ICLASS.EQ.2)THEN + CALL MOTIF2(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),CUT,MATCHP(MOTIF),MATCHS(MOTIF),IFOUND) + ELSE IF(ICLASS.EQ.3)THEN + CALL MOTIF3(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),CUT,MATCHP(MOTIF),MATCHS(MOTIF),IFOUND,MAT3, + + IDME) + ELSE IF(ICLASS.EQ.4)THEN + CALL MOTIF4(SEQ,IDSEQ,ILEN,START(MOTIF), + + IEND(MOTIF),WEIGHT(IWT),CUT,MATCHP(MOTIF),MATCHS(MOTIF), + + IFOUND,IDM) + ELSE IF(ICLASS.EQ.5)THEN + CALL MOTIF4(SEQ,IDSEQ,ILEN,START(MOTIF), + + IEND(MOTIF),WEIGHT(IWT),CUT,MATCHP(MOTIF),MATCHS(MOTIF), + + IFOUND,IDM) + ELSE IF(ICLASS.EQ.6)THEN + CALL MOTIF6(SEQ,IDSEQ,MAT4,LENGTH(MOTIF),START(MOTIF), + + IEND(MOTIF),RANGET(MOTIF),RANGEM(MOTIF), + + CUTOFF(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF), + + IENTRY(MOTIF),IFOUND,MATCHQ(MOTIF), + + LAST5(MOTIF),LAST3S(MOTIF),LAST3E(MOTIF),IDM) + ELSE IF(ICLASS.EQ.7)THEN + CALL MOTIF1(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF),IFOUND, + + CUTOFF(MOTIF),1) + ELSE IF(ICLASS.EQ.8)THEN + CALL MOTIF8(SEQ,IDSEQ,MAT2,LENGTH(MOTIF),START(MOTIF), + + IEND(MOTIF),RANGET(MOTIF),RANGEM(MOTIF), + + CUTOFF(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF), + + IENTRY(MOTIF),IFOUND,MATCHQ(MOTIF), + + LAST5(MOTIF),LAST3S(MOTIF),LAST3E(MOTIF),IDME) + ELSE + WRITE(KBOUT,*)'UNKNOWN CLASS!!' + END IF +C +C + END IF +C +C +C MATCH FOUND WHEN MATCH WANTED ? +C +C +C + IF(((IFOUND.EQ.0).AND.(COMB.NE.'N')).OR. + + ((IFOUND.GT.0).AND.(COMB.EQ.'N')))THEN +C +C NO SO GO BACK OR SIDEWAYS ONE MOTIF +C +C + CALL BAKSID(CLASS,LENGTH,CUTOFF,STRNGS,NMOT, + + MOTIF,ICLASS,ILEN,CUT,IWT,ISTRST,WTSTR, + + RELMOT,START,IEND,MATCHQ,RANGES,RANGEL,RELEND,IRET,MATCHP, + + COMBIN,COMB) +C +C +C IF CANT GO BACK ANY FURTHER QUIT + IF(IRET.NE.0)RETURN +C +C + ELSE +C +C +C MATCH FOUND. +C +C +C PREPARE FOR NEXT SEARCH THIS MOTIF BY INCREMENTING POINTER +C TO SEARCH RANGE (NOT FOR CLASS 6 WHICH IS HANDLED BY MOTIF6) +C +C + IF(COMB.EQ.'N')THEN + START(MOTIF) = -9 + ELSE +C IF((ICLASS.NE.6).AND.(ICLASS.NE.8)) +C REPLACE LAST LINE 7-7-89 + IF(ICLASS.NE.6)START(MOTIF) = MATCHP(MOTIF) + 1 +C ERROR????????? + IF(ICLASS.EQ.7)START(MOTIF) = MATCHP(MOTIF) + CUTOFF(MOTIF) + END IF +C +C TRY GOING FORWARD ONE MOTIF +C +C + CALL FORWAD(CLASS,LENGTH,CUTOFF,NMOT, + + MOTIF,ICLASS,ILEN,CUT,IWT,RELMOT,START,IEND, + + RANGES,RANGEL,STRNGS,ISTRST,WTSTR,IDSEQ,IENTRY, + + RANGET,RANGEM,START2,IEND2,MATCHQ,RELEND,MATCHP,IDSPLY, + + COMBIN,COMB) +C +C +C +C IS THIS THE LAST MOTIF? IF SO DISPLAY THE MATCH +C +C + IF(IDSPLY.EQ.1)THEN + ITOTAL = ITOTAL + 1 + CALL DSPLAY(MATCHP,LENGTH,NMOT,SEQ,IDSEQ,IDEVOT, + + CLASS,MATCHQ,MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IOPT, + + XMAX,XMIN,YMAX,YMIN,KSTART,MATCHS,CUTSCR,MINSCR,MAXSCR, + + PMINT,PMAXT,PROBT,IDM,MAT1,IDM,MAT2,IDME,MAT3,IDM, + + MAT4,IDM,WEIGHT,MAXWTS,WTSTR,CUTOFF,PMINC,KEYNS) +C +C +C HORRIBLE SPECIAL CASE - IF LAST MOTIF IS NOTTED WE MUST MOVE BACK AFTER +C DISPLAY + IF(COMB.EQ.'N')THEN + CALL BAKSID(CLASS,LENGTH,CUTOFF,STRNGS,NMOT, + + MOTIF,ICLASS,ILEN,CUT,IWT,ISTRST,WTSTR, + + RELMOT,START,IEND,MATCHQ,RANGES,RANGEL,RELEND,IRET,MATCHP, + + COMBIN,COMB) +C +C +C IF CANT GO BACK ANY FURTHER QUIT + IF(IRET.NE.0)RETURN + END IF + END IF +C +C + END IF +C +C +C GO BACK FOR NEXT SEARCH +C +C + GO TO 100 + END +C********************************************************************* + SUBROUTINE DSPLAY(MATCHP,LENGTH,NMOT,SEQ,IDSEQ,IDEV, + +CLASS,MATCHQ,MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IOPT, + +XMAX,XMIN,YMAX,YMIN,KSTART,MATCHS,CUTSCR,MINSCR,MAXSCR, + +PMINT,PMAXT,PROBT,IDM,MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3, + +MAT4,IDMAT4,WEIGHT,MAXWTS,WTSTR,CUTOFF,PMINC,KEYNS) + INTEGER MATCHP(NMOT),LENGTH(NMOT),CLASS(NMOT) + INTEGER MATCHQ(NMOT) + CHARACTER SEQ(IDSEQ) + REAL MATCHS(NMOT),MINSCR,MAXSCR + REAL WEIGHT(MAXWTS),CUTOFF(NMOT) + INTEGER MAT1(IDMAT1,IDMAT1),MAT2(IDMAT2,IDMAT2) + INTEGER MAT3(IDMAT3,IDMAT3),MAT4(IDMAT4,IDMAT4) + INTEGER WTSTR(NMOT) + CHARACTER*(*) KEYNS(NMOT) + EXTERNAL PSCORE +C ADD SCORES + T = 0. + DO 10 I = 1,NMOT + IF(MATCHP(I).NE.0) T = T + MATCHS(I) +10 CONTINUE + POBS = 1.0 + IF((PMINC.LT.1.0).OR.(IOPT.EQ.3))THEN +C CALC PROB + DO 20 I = 1,NMOT + IF(MATCHP(I).NE.0)THEN + CALL GETP(CLASS(I),SEQ(MATCHP(I)+KSTART-1),LENGTH(I), + + IDM,MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + + WEIGHT(MAX(1,WTSTR(I)))) + PROB = PSCORE(MATCHS(I)) + POBS = POBS * PROB + END IF +20 CONTINUE + IF((PMINC.LT.1.0).AND.(POBS.GT.PMINC))RETURN + END IF +C + IF(T.GT.MAXSCR) MAXSCR = T + IF(T.LT.MINSCR) MINSCR = T + IF(T.LT.CUTSCR) RETURN +C + IF(IOPT.EQ.1)THEN + WRITE(IDEV,1001) +1001 FORMAT(' Match') + DO 100 I=1,NMOT + J = I +C CHECK FOR NO MATCH (NEEDED FOR ORED MOTIFS) + IF(MATCHP(J).NE.0)THEN + WRITE(IDEV,1000)MATCHP(J)+KSTART-1,KEYNS(I) + WRITE(IDEV,1002)(SEQ(K),K=MATCHP(J),MATCHP(J)+LENGTH(J)-1) +C STEM ? + IF(CLASS(J).EQ.6)THEN + WRITE(IDEV,1002)(SEQ(K),K=MATCHQ(J),MATCHQ(J)-LENGTH(J)+1,-1) + WRITE(IDEV,1000)MATCHQ(J)+KSTART-1 + END IF +C REPEAT ? + IF(CLASS(J).EQ.8)THEN + WRITE(IDEV,1002)(SEQ(K),K=MATCHQ(J),MATCHQ(J)+LENGTH(J)-1) + WRITE(IDEV,1000)MATCHQ(J)+KSTART-1 + END IF + END IF +100 CONTINUE +1000 FORMAT(' ',I7,' ',A8) +1002 FORMAT(' ',60A1) + IF(PMINC.LT.1.0)WRITE(IDEV,1004)POBS +1004 FORMAT(' Probability =',E10.4) + RETURN + END IF + IF(IOPT.EQ.3)THEN +C MARK THE POSITION OF THE FIRST NON-ZERO MATCH ONLY + DO 200 I = 1,NMOT + IF(MATCHP(I).NE.0)THEN + X = MATCHP(I) + KSTART - 1 + Y = LOG(1.-POBS) + CALL VECTOM + CALL LINE(X,X,YMIN,Y,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL VT100M + GO TO 201 + END IF +200 CONTINUE +201 CONTINUE + END IF + IF(IOPT.EQ.2)THEN + MINP = 999999 + MAXP = -999999 + DO 300 I = 1, NMOT + K = MATCHP(I) + IF(K.NE.0)THEN + IF(K.LT.MINP)MINP = K + K = K + LENGTH(I) - 1 +C INVERTED REPEAT ? + IF(CLASS(I).EQ.6) K = MATCHQ(I) +C REPEAT ? + IF(CLASS(I).EQ.8) K = MATCHQ(I) + LENGTH(I) - 1 + IF(K.GT.MAXP)MAXP = K + END IF +300 CONTINUE + WRITE(IDEV,1000)MINP+KSTART-1,KEYNS(1) + WRITE(IDEV,1002) + + ((SEQ(K1),K1=K2,MIN(K2+59,MAXP)),K2=MINP,MAXP,60) + IF(PMINC.LT.1.0)WRITE(IDEV,1004)POBS + RETURN + END IF + IF(IOPT.EQ.4)THEN + MINP = 999999 + MAXP = -999999 + K1 = 0 + DO 400 I = 1, NMOT + K = MATCHP(I) + IF(K.NE.0)THEN + IF(K1.EQ.0) INAM = I + K1 = 1 + IF(K.LT.MINP)MINP = K + K = K + LENGTH(I) - 1 +C INVERTED REPEAT ? + IF(CLASS(I).EQ.6) K = MATCHQ(I) +C REPEAT ? + IF(CLASS(I).EQ.8) K = MATCHQ(I) + LENGTH(I) - 1 + IF(K.GT.MAXP)MAXP = K + END IF +400 CONTINUE + CALL EFTOUT(KEYNS(INAM),MINP+KSTART-1,MAXP+KSTART-1,IDEV) + END IF + END +C********************************************************************* + SUBROUTINE MOTIF3(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND,CUTOFF, + +MATCHP,MATCHS,IFOUND,MATRIC,IDM) + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER MATRIC(IDM,IDM) + REAL MATCHS + IFOUND = 0 + IF(ISTART.LT.1)ISTART=1 + IF(ISTART.GT.IDIM1)RETURN + CALL SQFIT5(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND,CUTOFF,MATCHS, + +IFOUND,MATRIC,IDM) + IF(IFOUND.EQ.0)RETURN +C SAVE MATCH POSITION + MATCHP = IFOUND + RETURN + END +C********************************************************************* + SUBROUTINE MOTIF4(SEQ,IDIM1,LENGTH,ISTART,IEND, + +WEIGHT,CUTOFF,MATCHP,MATCHS,IFOUND,IDM) + REAL MATCHS + CHARACTER SEQ(IDIM1) + IFOUND = 0 + IF(ISTART.LT.1)ISTART=1 + L1 = IEND-ISTART+1 + IF(ISTART.GT.IDIM1)RETURN + IF(L1.LT.LENGTH)RETURN + CALL FMOT4(SEQ(ISTART),L1,WEIGHT,LENGTH,CUTOFF,SCORE,IFOUND,IDM) + IF(IFOUND.EQ.0)RETURN +C SAVE MATCH POSITION + MATCHP = ISTART+IFOUND-1 + MATCHS = SCORE + RETURN + END +C********************************************************************* + SUBROUTINE FMOT4(SEQ,IDIM,WT,LENGTH,CUTOFF,SUM,IFOUND,IDM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + REAL WT(IDM,LENGTH) + INTEGER CTONUM + EXTERNAL CTONUM + DO 10 I=1,IDIM-LENGTH+1 + SUM=0. + K=0 + DO 5 J=I,I+LENGTH-1 + K=K+1 + IP = CTONUM(SEQ(J)) + SUM=SUM+WT(IP,K) +5 CONTINUE + IF(SUM.GE.CUTOFF)THEN + IFOUND = I + RETURN + END IF +10 CONTINUE + IFOUND = 0 + RETURN + END +C********************************************************************* + SUBROUTINE MOTIF6(SEQ,IDSEQ,MATRIX,LENGTH,I5STAR,I5END, + + I3STAR,I3END,CUTOFF,MATCHP,MATCHS, + + IENTRY,IFOUND,MATCHQ, + + LAST5,LAST3S,LAST3E,IDM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ) + INTEGER MATRIX(IDM,IDM) + REAL MATCHS + EXTERNAL LOOP +C WE HAVE A START POSITION FOR THE 5' END OF THE 5' END OF +C A POTENTIAL STEM I5STAR AND AN END DEFINED BY A RANGE I5END +C WE HAVE A STEM LENGTH LENGTH +C WE HAVE A RANGE OF POSITIONS FOR THE 3' STEM TO START +C I3STAR TO I3END +C TRY THE TIGHTEST LOOPS FIRST +C BUT FIRST WE MAY HAVE TO FINISH A PREVIOUS SEARCH +C THIS IS DENOTED BY IENTRY NE 0. +C NOTE IENTRY IS ALSO USED TO RETURN THE 3' MATCH POSITION + ICUT = CUTOFF +C WRITE(*,*)'ICUT',ICUT + IFOUND = 0 + JENTRY = IENTRY + IENTRY = 0 + IF(I5STAR.LT.1)I5STAR=1 + IF((I5STAR+I3STAR-1).GT.IDSEQ)RETURN + IF(JENTRY.NE.0)THEN + I1 = LAST5 +C WRITE(*,*)'I5STAR,I5END',I5STAR,I5END +C WRITE(*,*)'I1,LAST3S,LAST3E',I1,LAST3S,LAST3E + DO 50 J=LAST3S+1,LAST3E + J1 = J + ISUM = LOOP(SEQ,IDSEQ,MATRIX,LENGTH,I1,J1,IDM) +C RETURN IF GOOD ENOUGH + IF(ISUM.GE.CUTOFF)THEN + MATCHP = I1 + IENTRY = J1 + MATCHQ = J1 + MATCHS = ISUM + IFOUND = MATCHP + LAST3S = J1 +CDEBUG +C WRITE(*,*)'MATCHP',MATCHP + RETURN + END IF +50 CONTINUE +C NOW MOVE 5' STEM START POSITION (WE HAVE JUST FINISHED THE LAST) +C TO THE LAST MATCH + 1 + I5STAR = MATCHP + 1 + END IF +C SET ENTRY FLAG TO ZERO TO SIGNIFY LAST SEARCH NOW COMPLETED + IENTRY = 0 + ISUM = 0 + LOOPI1 = I5STAR + IF((I5STAR+I3STAR-1).GT.IDSEQ)RETURN + LOOPI2 = MIN(IDSEQ,I5END) +C WRITE(*,*)'IDSEQ,LENGTH,I5STAR,I5END', +C +IDSEQ,LENGTH,I5STAR,I5END +C +C TRY ALL STEM STARTS FROM 5' START TO 5' END +C +C + DO 200 I = LOOPI1,LOOPI2 +C +C + I1 = I +C +C TRY ALL LOOPS FROM 3' START TO 3' END +C + LOOPJ1 = I+I3STAR-1 + IF(LOOPJ1.GT.IDSEQ)RETURN + LOOPJ2 = MIN(IDSEQ,I+I3END-1) +C WRITE(*,*)'I3STAR,I3END',I3STAR,I3END +C +C +C + DO 100 J = LOOPJ1,LOOPJ2 +C +C +C + J1 = J +C IN LOOP NOTE THAT +C THE 5' END POINTER I1 GOES FORWARDS +C THE 3' END POINTER J1 GOES BACKWARDS +C + ISUM = LOOP(SEQ,IDSEQ,MATRIX,LENGTH,I1,J1,IDM) +C RETURN IF GOOD ENOUGH +C WRITE(*,*)ISUM + IF(ISUM.GE.ICUT)THEN + MATCHP = I1 + IENTRY = J1 + MATCHQ = J1 + MATCHS = ISUM + IFOUND = MATCHP +C SAVE CURRENT POSITION FOR LATER ENTRIES + LAST5 = I1 + LAST3S = J1 + LAST3E = LOOPJ2 +CDEBUG +C WRITE(*,*)'MATCHP',MATCHP + RETURN + END IF +100 CONTINUE +200 CONTINUE +CDEBUG +C WRITE(*,*)'MATCHPEND',MATCHP + END +C********************************************************************* + INTEGER FUNCTION LOOP(SEQ,IDSEQ,MATRIX,LENGTH,I5P,I3P,IDM) + CHARACTER SEQ(IDSEQ) + INTEGER MATRIX(IDM,IDM),CTONUM + EXTERNAL CTONUM +C THE 5' END POINTER GOES FORWARDS +C THE 3' END POINTER GOES BACKWARDS + L=0 + I5=I5P-1 + I3=I3P+1 + DO 100 I=1,LENGTH + I5 = I5 + 1 + I3 = I3 - 1 +C WRITE(*,*)'I5,I3',I5,I3 + L5 = CTONUM(SEQ(I5)) + L3 = CTONUM(SEQ(I3)) + L = L + MATRIX(L5,L3) +100 CONTINUE + LOOP = L + END + SUBROUTINE MOTIF8(SEQ,IDSEQ,MATRIX,LENGTH,I5STAR,I5END, + + I3STAR,I3END,CUTOFF,MATCHP,MATCHS, + + IENTRY,IFOUND,MATCHQ, + + LAST5,LAST3S,LAST3E,IDM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ) + INTEGER MATRIX(IDM,IDM),REPEET + REAL MATCHS + EXTERNAL REPEET +C WE HAVE A START POSITION FOR THE 5' END OF THE 5' END OF +C A POTENTIAL REPEAT I5STAR AND AN END DEFINED BY A RANGE I5END +C WE HAVE A REPEAT LENGTH LENGTH +C WE HAVE A RANGE OF POSITIONS FOR THE 3' STEM TO START +C I3STAR TO I3END +C TRY THE TIGHTEST LOOPS FIRST +C BUT FIRST WE MAY HAVE TO FINISH A PREVIOUS SEARCH +C THIS IS DENOTED BY IENTRY NE 0. +C NOTE IENTRY IS ALSO USED TO RETURN THE 3' MATCH POSITION + ICUT = CUTOFF +C WRITE(*,*)'ICUT',ICUT + IFOUND = 0 + JENTRY = IENTRY + IENTRY = 0 + IF(I5STAR.LT.1)I5STAR=1 + IF((I5STAR+I3STAR+LENGTH-2).GT.IDSEQ)RETURN + IF(JENTRY.NE.0)THEN + I1 = LAST5 +C WRITE(*,*)'I1,LAST3S,LAST3E',I1,LAST3S,LAST3E + DO 50 J=LAST3S+1,LAST3E + J1 = J + ISUM = REPEET(SEQ,IDSEQ,MATRIX,LENGTH,I1,J1,IDM) +C RETURN IF GOOD ENOUGH + IF(ISUM.GE.ICUT)THEN + MATCHP = I1 + IENTRY = J1 + MATCHQ = J1 + MATCHS = ISUM + IFOUND = MATCHP + LAST3S = J1 + RETURN + END IF +50 CONTINUE +C NOW MOVE 5' STEM START POSITION (WE HAVE JUST FINISHED THE LAST) +C TO THE LAST MATCH + 1 + I5STAR = MATCHP + 1 + END IF +C SET ENTRY FLAG TO ZERO TO SIGNIFY LAST SEARCH NOW COMPLETED + IENTRY = 0 + ISUM = 0 + LOOPI1 = I5STAR + IF((I5STAR+I3STAR+LENGTH-2).GT.IDSEQ)RETURN + LOOPI2 = MIN(IDSEQ-2*LENGTH+1,I5END) +C WRITE(*,*)'IDSEQ,LENGTH,I5STAR,I5END', +C +IDSEQ,LENGTH,I5STAR,I5END +C +C TRY ALL STEM STARTS FROM 5' START TO 5' END +C +C + DO 200 I = LOOPI1,LOOPI2 +C +C + I1 = I +C +C TRY ALL LOOPS FROM 3' START TO 3' END +C + LOOPJ1 = I + I3STAR -1 + IF((LOOPJ1+LENGTH-1).GT.IDSEQ)RETURN + LOOPJ2 = MIN(IDSEQ-LENGTH+1,I+I3END-1) +C WRITE(*,*)'I3STAR,I3END',I3STAR,I3END +C +C +C + DO 100 J = LOOPJ1,LOOPJ2 +C +C +C + J1 = J +C IN REPEAT NOTE THAT +C THE 5' END POINTER I1 GOES FORWARDS +C THE 3' END POINTER J1 GOES FORWARDS +C + ISUM = REPEET(SEQ,IDSEQ,MATRIX,LENGTH,I1,J1,IDM) +C RETURN IF GOOD ENOUGH +C WRITE(*,*)ISUM + IF(ISUM.GE.ICUT)THEN + MATCHP = I1 + IENTRY = J1 + MATCHQ = J1 + MATCHS = ISUM + IFOUND = MATCHP +C SAVE CURRENT POSITION FOR LATER ENTRIES + LAST5 = I1 + LAST3S = J1 + LAST3E = LOOPJ2 + RETURN + END IF +100 CONTINUE +200 CONTINUE + END +C********************************************************************* + INTEGER FUNCTION REPEET(SEQ,IDSEQ,MATRIX,LENGTH,I5P,I3P,IDM) + CHARACTER SEQ(IDSEQ) + INTEGER MATRIX(IDM,IDM),DTONUM + EXTERNAL DTONUM +C THE 5' END POINTER GOES FORWARDS +C THE 3' END POINTER GOES FORWARDS + L=0 + I5=I5P-1 + I3=I3P-1 + DO 100 I=1,LENGTH + I5 = I5 + 1 + I3 = I3 + 1 +C WRITE(*,*)'I5,I3',I5,I3 + L5 = DTONUM(SEQ(I5)) + L3 = DTONUM(SEQ(I3)) + L = L + MATRIX(L5,L3) +100 CONTINUE + REPEET = L + END + SUBROUTINE SQFIT5(SEQ,IDIM1,STRING,IDIM2, + 1IS,IE,MINSC,MATCHS,IFOUND,MATRIC,IDM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2) + REAL MATCHS,MINSC + INTEGER MATRIC(IDM,IDM),DTONUM + EXTERNAL DTONUM + MINSCR = MINSC + IDIF=(IE-IS+2)-IDIM2 + IPSEQ=IS + DO 200 I=1,IDIF + NTOT=0 + IP=IPSEQ + DO 100 J=1,IDIM2 + NTOT = NTOT + MATRIC(DTONUM(SEQ(IP)),DTONUM(STRING(J))) + IP=IP+1 +100 CONTINUE +C END OF COUNTING FOR THIS POSITION.IS TOTAL HIGH ENOUGH? + IF(NTOT.GE.MINSCR)THEN + MATCHS = NTOT + IFOUND = IP-IDIM2 + RETURN + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + IFOUND = 0 + RETURN + END + SUBROUTINE SETCMP(SEQ,IDIM,IDM) + CHARACTER SEQ(IDIM) + PARAMETER (MAXCHR = 17) + INTEGER DTONUM + EXTERNAL DTONUM + COMMON /COMPC/COMP(MAXCHR) + SAVE /COMPC/ + DO 10 I = 1,IDM + COMP(I) = 0.0 +10 CONTINUE + DO 20 I = 1,IDIM + J = DTONUM(SEQ(I)) + COMP(J) = COMP(J) + 1. +20 CONTINUE + T = MAX(1,IDIM) + DO 30 I = 1,IDM + COMP(I) = COMP(I) / T +30 CONTINUE + END diff --git a/src/staden/patternnc.f b/src/staden/patternnc.f new file mode 100644 index 0000000..d4afb63 --- /dev/null +++ b/src/staden/patternnc.f @@ -0,0 +1,1668 @@ +C 9-11-90 very many changes concerning use of file of file names +C and switching radio to radion +C 3-7-91 Removed annotation "filename" from pattern files +C 4-7-91 replaced embout by eftout +C 18-7-91 added titles to pattern files +C 2-3-92 set filnam = ' ' for some calls to openf1 + SUBROUTINE GETMF(KBIN,KBOUT,STRING,MAXSTR,ISTRNG, + +LENGTH,MAXMOT,CLASS,RELMOT,RANGES,RANGEL,RANGET,RANGEM, + +STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF,IDEV,WTSTR,JDEV,IOK, + +RELEND,IDSEQ,IDEVSV,IDM,COMBIN,MAXCLS, + +MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + +PMINT,PMAXT,PROBT,EXPTT, + +IHELPS,IHELPE,HELPF,IDEVH,KEYNS,NAMSAV,FILNAM,IPROB,TITLE) +C MAT1 SIMPLE IDENTITY +C MAT2 IUB SCORES 0-1 +C MAT3 IUB SCORES 0-36 +C MAT4 INVERTED REPEAT + INTEGER LENGTH(MAXMOT),CLASS(MAXMOT),RELMOT(MAXMOT) + INTEGER RANGES(MAXMOT),RANGEL(MAXMOT),STRNGS(MAXMOT) + INTEGER WTSTR(MAXMOT),RANGET(MAXMOT),RANGEM(MAXMOT) + INTEGER RELEND(MAXMOT) + CHARACTER STRING(MAXSTR),FILNAM*(*),HELPF*(*) + CHARACTER*(*) KEYNS(MAXMOT),TITLE + REAL WEIGHT(MAXWTS),CUTOFF(MAXMOT) + CHARACTER COMBIN(MAXMOT),TCLASS + INTEGER MAT1(IDMAT1,IDMAT1),MAT2(IDMAT2,IDMAT2) + INTEGER MAT3(IDMAT3,IDMAT3),MAT4(IDMAT4,IDMAT4) + CHARACTER*(*) NAMSAV(MAXMOT) + EXTERNAL PSCORE +C GETS PATTERN DEFINITIONS IN TERMS OF MOTIFS + PMINT = 1.0 + PMAXT = 1.0 + PROBT = 1.0 + EXPTS = 0.0 + PROBS = 0.0 + PMINS = 0.0 + PMAXS = 0.0 + EXPTT = 1.0 + IOK = 1 + DO 10 I=1,MAXMOT + RELEND(I) = 5 + COMBIN(I) = 'A' +10 CONTINUE + RANGES(1) = 0 + RANGEL(1) = IDSEQ + IREL = 0 +C COUNT MOTIFS + NMOT = 0 + MOTIF = 0 +C SET POINTER TO SEARCH STRINGS ARRAY + ISTRNG = 1 +C SET POINTER TO WEIGHT ARRAY + IWT = 1 +C get title + READ(JDEV,1000,ERR=901,END=901)TITLE + 1000 FORMAT(A) +100 CONTINUE + CALL GETCLS(JDEV,KBOUT,ICLASS,TCLASS,KEYNS(MOTIF+1)) +1001 FORMAT(I7) + IF(ICLASS.LT.0) GO TO 901 + IF(ICLASS.GT.MAXCLS)GO TO 901 + IF(ICLASS.EQ.0)GO TO 900 + IF((TCLASS.EQ.'O').AND.(NMOT.LT.1))THEN + WRITE(KBOUT,*)'CANNOT OR FIRST MOTIF' + GO TO 901 + END IF + IF((TCLASS.EQ.'N').AND.(NMOT.LT.1))THEN + WRITE(KBOUT,*)'CANNOT NOT FIRST MOTIF' + GO TO 901 + END IF + IF((TCLASS.EQ.'O').AND.(COMBIN(MOTIF).EQ.'N'))THEN + WRITE(KBOUT,*)'CANNOT OR WITH NOTTED MOTIF' + GO TO 901 + END IF + NMOT = NMOT + 1 + MOTIF = MOTIF + 1 + IF(NMOT.GT.MAXMOT)THEN + WRITE(KBOUT,*)'MAXIMUM NUMBER OF MOTIFS EXCEEDED' + GO TO 901 + END IF + CLASS(MOTIF) = ICLASS + COMBIN(MOTIF) = TCLASS +C IF NOT THE FIRST MOTIF, AND AN ANDED MOTIF OR THE FIRST IN A LIST OF ORS +C GET ITS RANGE ETC + IF((MOTIF.GT.1).AND.(COMBIN(MOTIF).NE.'O'))THEN + READ(JDEV,1001,ERR=901)IREL + IF(IREL.LT.0)GO TO 901 + IF(IREL.LT.1)GO TO 901 + IF(IREL.GT.MOTIF-1)THEN + WRITE(KBOUT,*)'CAN ONLY REFER BACK' + GO TO 901 + END IF + IF(COMBIN(IREL).NE.'A')THEN + WRITE(KBOUT,*)'CAN ONLY REFER TO AN ANDED MOTIF' + GO TO 901 + END IF + RELMOT(MOTIF) = IREL + IF(CLASS(IREL).EQ.6)THEN + READ(JDEV,1001,ERR=901)MEND + IF(MEND.LT.0)GO TO 901 + RELEND(MOTIF) = 5 + IF(MEND.EQ.3)RELEND(MOTIF) = 3 + READ(JDEV,1001,ERR=901)ID + IF(ID.LT.0)GO TO 901 + ELSE + READ(JDEV,1001,ERR=901)ID + END IF + READ(JDEV,1001,ERR=901)IR + IF(IR.LT.0)GO TO 901 + RANGES(MOTIF) = ID - 1 + RANGEL(MOTIF) = IR + ELSE IF(COMBIN(MOTIF).EQ.'O')THEN +C NEED TO SET RANGES TO THOSE OF THE FIRST IN A SET OF ORED MOTIFS +C SET TO THOSE OF THE PREVIOUS MOTIF BECAUSE IT MUST BE THE SAME + RANGES(MOTIF) = RANGES(MOTIF-1) + RANGEL(MOTIF) = RANGEL(MOTIF-1) + RELEND(MOTIF) = RELEND(MOTIF-1) + RELMOT(MOTIF) = IREL + END IF +C KEYNS(MOTIF) = KEYNAM + XRAN = 1.0 +C NOW GET DETAILS SPECIFIC TO EACH CLASS OF MOTIF + CALL GETMC(KBIN,KBOUT,STRING,MAXSTR,ISTRNG, + +LENGTH,MAXMOT,CLASS,RELMOT,RANGES,RANGEL,RANGET,RANGEM, + +STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF,IDEV,WTSTR,JDEV,IOK, + +RELEND,IDSEQ,IDEVSV,IDM,COMBIN,MAXCLS, + +MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + +PMINT,PMAXT,PROBT,EXPTT,EXPTS,PROBS,PMINS,PMAXS, + +IHELPS,IHELPE,HELPF,IDEVH,MOTIF,IWT,NAMSAV,FILNAM,IPROB) + IF(IOK.EQ.0) GO TO 100 +901 CONTINUE + WRITE(KBOUT,*)' Error in pattern definition' + IOK = 1 + CLOSE(UNIT=JDEV) + RETURN +900 CONTINUE + EXPTT = EXPTT * EXPTS + PROBT = PROBT * PROBS + PMINT = PMINT * PMINS + PMAXT = PMAXT * PMAXS + CLOSE(UNIT=JDEV) + DO 899 I = 2,NMOT + IF((CLASS(I).EQ.6).OR.(CLASS(I).EQ.8)) THEN + RANGEL(I) = RANGEL(I) + 1 + ELSE + RANGEL(I) = RANGEL(I) + LENGTH(I) + END IF +899 CONTINUE +C RETURN STRING LENGTH + MAXSTR = ISTRNG - 1 + IOK = 0 + END + SUBROUTINE GETMC(KBIN,KBOUT,STRING,MAXSTR,ISTRNG, + +LENGTH,MAXMOT,CLASS,RELMOT,RANGES,RANGEL,RANGET,RANGEM, + +STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF,IDEV,WTSTR,JDEV,IOK, + +RELEND,IDSEQ,IDEVSV,IDM,COMBIN,MAXCLS, + +MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + +PMINT,PMAXT,PROBT,EXPTT,EXPTS,PROBS,PMINS,PMAXS, + +IHELPS,IHELPE,HELPF,IDEVH,MOTIF,IWT,NAMSAV,FILNAM,IPROB) + INTEGER LENGTH(MAXMOT),CLASS(MAXMOT),RELMOT(MAXMOT) + INTEGER RANGES(MAXMOT),RANGEL(MAXMOT),STRNGS(MAXMOT) + INTEGER WTSTR(MAXMOT),RANGET(MAXMOT),RANGEM(MAXMOT) + INTEGER RELEND(MAXMOT) + CHARACTER STRING(MAXSTR),FILNAM*(*),HELPF*(*) + REAL WEIGHT(MAXWTS),CUTOFF(MAXMOT) + CHARACTER COMBIN(MAXMOT) + INTEGER MAT1(IDMAT1,IDMAT1),MAT2(IDMAT2,IDMAT2) + INTEGER MAT3(IDMAT3,IDMAT3),MAT4(IDMAT4,IDMAT4) + CHARACTER*(*) NAMSAV(MAXMOT) + EXTERNAL PSCORE + XRAN = 1.0 + PMIN = 1. + PMAX = 1. + PROB = 1. +C NOW GET DETAILS SPECIFIC TO EACH CLASS OF MOTIF + IF(CLASS(MOTIF).EQ.1)THEN + MXSTR = MAXSTR - ISTRNG + 1 + CALL GETMT1(STRING(ISTRNG),MXSTR,LENGTH(MOTIF),KBIN,KBOUT, + + IOK,JDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM, + + MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + + WEIGHT(IWT)) + SCMIN = LENGTH(MOTIF) + PROB = PSCORE(SCMIN) + PMIN = PROB + PMAX = PROB + END IF + STRNGS(MOTIF) = ISTRNG + ISTRNG = ISTRNG + LENGTH(MOTIF) + CUTOFF(MOTIF) = LENGTH(MOTIF) + ELSE IF(CLASS(MOTIF).EQ.2)THEN + MXSTR = MAXSTR - ISTRNG + 1 + CALL GETMT2(STRING(ISTRNG),MXSTR,LENGTH(MOTIF), + + CUTOFF(MOTIF),KBIN,KBOUT,IOK,JDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM, + + MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + + WEIGHT(IWT)) + SCMIN = CUTOFF(MOTIF) + SCMAX = LENGTH(MOTIF) + PROB = PSCORE(SCMIN) + PMIN = PROB + PMAX = PSCORE(SCMAX) + END IF + STRNGS(MOTIF) = ISTRNG + ISTRNG = ISTRNG + LENGTH(MOTIF) + ELSE IF(CLASS(MOTIF).EQ.3)THEN + MXSTR = MAXSTR - ISTRNG + 1 + CALL GETMT3(STRING(ISTRNG),MXSTR,LENGTH(MOTIF), + + CUTOFF(MOTIF),MAT3,IDMAT3,KBIN,KBOUT,IOK,JDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM, + + MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + + WEIGHT(IWT)) + SCMIN = CUTOFF(MOTIF) + SCMAX = LENGTH(MOTIF) + PROB = PSCORE(SCMIN) + PMAX = PSCORE(SCMAX) + PMIN = PROB + END IF + STRNGS(MOTIF) = ISTRNG + ISTRNG = ISTRNG + LENGTH(MOTIF) + ELSE IF(CLASS(MOTIF).EQ.4)THEN + MXWT = MAXWTS - IWT + 1 + CALL GETMT4(WEIGHT(IWT),MXWT,LENGTH(MOTIF),CUTOFF(MOTIF), + + SCMAX,IDEV,KBIN,KBOUT,IOK,JDEV,FILNAM,IDM, + + IHELPS,IHELPE,HELPF,IDEVH,IPROB) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM, + + MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + + WEIGHT(IWT)) + SCMIN = CUTOFF(MOTIF) + PROB = PSCORE(SCMIN) + PMAX = PSCORE(SCMAX) + PMIN = PROB + END IF + WTSTR(MOTIF) = IWT + IWT = IWT + LENGTH(MOTIF)*IDM + NAMSAV(MOTIF) = FILNAM + ELSE IF(CLASS(MOTIF).EQ.5)THEN + MXWT = MAXWTS - IWT + 1 + CALL GETMT5(WEIGHT(IWT),MXWT,LENGTH(MOTIF),CUTOFF(MOTIF), + + SCMAX,IDEV,KBIN,KBOUT,IOK,JDEV,FILNAM,IDM, + + IHELPS,IHELPE,HELPF,IDEVH,IPROB) + IF(IOK.NE.0)GO TO 901 + SCMIN = CUTOFF(MOTIF) + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM, + + MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + + WEIGHT(IWT)) + SCMIN = CUTOFF(MOTIF) + PROB = PSCORE(SCMIN) + PMAX = PSCORE(SCMAX) + PMIN = PROB + END IF + WTSTR(MOTIF) = IWT + IWT = IWT + LENGTH(MOTIF)*IDM + NAMSAV(MOTIF) = FILNAM + ELSE IF(CLASS(MOTIF).EQ.6)THEN + CALL GETMT6(RANGET(MOTIF),RANGEM(MOTIF),LENGTH(MOTIF), + + CUTOFF(MOTIF),KBIN,KBOUT,JDEV,IOK, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM, + + MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + + WEIGHT(IWT)) + SCMIN = CUTOFF(MOTIF) + SCMAX = LENGTH(MOTIF)*2 + PROB = PSCORE(SCMIN) + PMAX = PSCORE(SCMAX) + PMIN = PROB + END IF + XRAN = ABS(RANGEM(MOTIF))-ABS(RANGET(MOTIF))+1 + ELSE IF(CLASS(MOTIF).EQ.7)THEN + MXSTR = MAXSTR - ISTRNG + 1 + CALL GETMT7(STRING(ISTRNG),MXSTR,LENGTH(MOTIF),KBIN,KBOUT, + + IOK,JDEV,CUTOFF(MOTIF), + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM, + + MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + + WEIGHT(IWT)) + STRNGS(MOTIF) = ISTRNG + ISTRNG = ISTRNG + LENGTH(MOTIF) + SCMIN = LENGTH(MOTIF) + PROB = PSCORE(SCMIN) + PMIN = PROB + PMAX = PROB + END IF + XRAN = 1.0 / CUTOFF(MOTIF) + ELSE IF(CLASS(MOTIF).EQ.8)THEN + CALL GETMT8(RANGET(MOTIF),RANGEM(MOTIF),LENGTH(MOTIF), + + CUTOFF(MOTIF),KBIN,KBOUT,JDEV,IOK, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM, + + MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4, + + WEIGHT(IWT)) + SCMIN = CUTOFF(MOTIF) + SCMAX = LENGTH(MOTIF) + PROB = PSCORE(SCMIN) + PMAX = PSCORE(SCMAX) + PMIN = PROB + END IF + XRAN = ABS(RANGEM(MOTIF))-ABS(RANGET(MOTIF))+1 + END IF +C GET RANGE OF POSITIONS FOR PROBABILITY CALC + LRANGE = RANGEL(MOTIF) + 1 + IF(COMBIN(MOTIF).EQ.'A')THEN + IF(EXPTS.GT.0.0)THEN + EXPTT = EXPTT * EXPTS + PROBT = PROBT * PROBS + PMINT = PMINT * PMINS + PMAXT = PMAXT * PMAXS + END IF + EXPTS = LRANGE * XRAN * PROB + PROBS = PROB + PMINS = PMIN + PMAXS = PMAX + ELSE IF(COMBIN(MOTIF).EQ.'N')THEN + IF(EXPTS.GT.0.0)THEN + EXPTT = EXPTT * EXPTS + PROBT = PROBT * PROBS + PMINT = PMINT * PMINS + PMAXT = PMAXT * PMAXS + END IF + PMIN = 1.0 - PMIN + PMAX = 1.0 - PMAX + PROB = 1.0 - PROB + EXPTS = PROB ** (LRANGE * XRAN) + PROBS = PROB + PMINS = PMIN + PMAXS = PMAX + ELSE IF(COMBIN(MOTIF).EQ.'O')THEN + EXPTS = EXPTS + LRANGE * XRAN * PROB + PROBS = PROBS + PROB + PMINS = PMINS + PMIN + PMAXS = PMAXS + PMAX + END IF + IF(IPROB.EQ.0) CALL WRTSCR(SCMIN,PROB,KBOUT) + IOK = 0 + RETURN +901 CONTINUE + IOK = 1 + END +C********************************************************************* + SUBROUTINE GETMT1(STRING,MAXSTR,LENGTH,KBIN,KBOUT,IOK,JDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + CHARACTER NEW(50),STRING(MAXSTR) +C GETS DETAILS FOR MOTIF CLASS 1 + IOK = 1 + IF(KBIN.EQ.JDEV)THEN +10 CONTINUE + LENGTH = 0 + CALL GETSTR('String',STRING,NEW,50,LENGTH,KBOUT,KBIN,INFLAG) + IF(LENGTH.LT.1) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + CALL SQCOPY(NEW,STRING,LENGTH) + ELSE + LENGTH = MAXSTR + CALL ARRFIL(JDEV,STRING,LENGTH,KBOUT) + IF(LENGTH.LT.1)THEN + WRITE(KBOUT,*)'ZERO LENGTH STRING' + RETURN + END IF + END IF + IOK = 0 + RETURN + END +C********************************************************************* + SUBROUTINE GETMT2(STRING,MAXSTR,LENGTH,CUTOFF,KBIN,KBOUT,IOK,JDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + CHARACTER NEW(50),STRING(MAXSTR) + REAL MININ,MAXIN +C GETS DETAILS FOR MOTIF CLASS 2 + IOK = 1 + IF(KBIN.EQ.JDEV)THEN +10 CONTINUE + LENGTH = 0 + CALL GETSTR('string',STRING,NEW,50,LENGTH,KBOUT,KBIN,INFLAG) + IF(LENGTH.LT.1) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + CALL SQCOPY(NEW,STRING,LENGTH) + MININ = 1. + MAXIN = LENGTH + CUTOFF = LENGTH + CALL GETRL(MININ,MAXIN,CUTOFF,'Minimum matches', + + VALUE,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CUTOFF = VALUE + ELSE + LENGTH = MAXSTR + CALL ARRFIL(JDEV,STRING,LENGTH,KBOUT) + IF(LENGTH.LT.1)THEN + WRITE(KBOUT,*)'ZERO LENGTH STRING' + RETURN + END IF + READ(JDEV,1002,ERR=901)CUTOFF +1002 FORMAT(F10.0) + END IF + IOK = 0 + RETURN +901 CONTINUE + IOK = 1 + END +C********************************************************************* + SUBROUTINE GETMT3(STRING,MAXSTR,LENGTH,CUTOFF,MAT3,IDMAT3, + +KBIN,KBOUT,IOK,JDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + CHARACTER NEW(50),STRING(MAXSTR) + INTEGER MAT3(IDMAT3,IDMAT3) + REAL MININ,MAXIN +C GETS DETAILS FOR MOTIF CLASS 3 + IOK = 1 + IF(KBIN.EQ.JDEV)THEN +10 CONTINUE + LENGTH = 0 + CALL GETSTR('String',STRING,NEW,50,LENGTH,KBOUT,KBIN,INFLAG) + IF(LENGTH.LT.1) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + CALL SQCOPY(NEW,STRING,LENGTH) + CALL GTSCR(STRING,LENGTH,MAT3,IDMAT3,ISMIN,ISMAX) + MININ = ISMIN + MAXIN = ISMAX + CUTOFF = MAXIN + CALL GETRL(MININ,MAXIN,CUTOFF,'Minimum score', + + VALUE,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CUTOFF = VALUE + ELSE + LENGTH = MAXSTR + CALL ARRFIL(JDEV,STRING,LENGTH,KBOUT) + IF(LENGTH.LT.1)THEN + WRITE(KBOUT,*)'ZERO LENGTH STRING' + RETURN + END IF + READ(JDEV,1002,ERR=901)CUTOFF +1002 FORMAT(F10.0) + END IF + IOK = 0 + RETURN +901 CONTINUE + IOK = 1 + END + SUBROUTINE GETMT4(WEIGHT,MAXWTS,LENGTH,CUTOFF,YMAX,IDEV, + +KBIN,KBOUT,IOK,JDEV,FILNAM,IDM, + + IHELPS,IHELPE,HELPF,IDEVH,IPROB) + CHARACTER HELPF*(*) + PARAMETER (MAXLEN = 120, MAXCHR = 5) + REAL WEIGHT(MAXWTS) + CHARACTER FILNAM*(*) + INTEGER TOT(MAXLEN),SUM(MAXCHR,MAXLEN) + IF(JDEV.EQ.KBIN) THEN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,JDEV,KBOUT, + + 'Weight matrix file name', + + IHELPS,IHELPE,HELPF,IDEVH) + ELSE + CALL OPENF(IDEV,FILNAM,0,IOK,JDEV,KBOUT) + END IF + IF(IOK.NE.0)RETURN + LENGTH=MAXLEN + CALL RDWMT(TOT,SUM,MIDDLE,LENGTH,MAXLEN,CUTOFF,YMAX,IDEV, + + IOK,IDM,KBOUT,IPROB) + IF(IOK.NE.0)THEN + WRITE(KBOUT,*)' Error in weight matrix, option left' + RETURN + END IF + IF(CUTOFF.LT.0.0)CALL GETW(TOT,SUM,WEIGHT,LENGTH,IDM,MAXLEN) + IF(CUTOFF.GE.0.0)CALL GETW2(SUM,WEIGHT,LENGTH,IDM,MAXLEN) + END +C********************************************************************* + SUBROUTINE GETMT5(WEIGHT,MAXWTS,LENGTH,CUTOFF,SCMAX,IDEV, + +KBIN,KBOUT,IOK,JDEV,FILNAM,IDM, + + IHELPS,IHELPE,HELPF,IDEVH,IPROB) + CHARACTER HELPF*(*) + REAL WEIGHT(MAXWTS) + CHARACTER FILNAM*(*) + IF(JDEV.EQ.KBIN) THEN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,JDEV,KBOUT, + + 'Weight matrix file name', + + IHELPS,IHELPE,HELPF,IDEVH) + ELSE + CALL OPENF(IDEV,FILNAM,0,IOK,JDEV,KBOUT) + END IF + IF(IOK.NE.0)RETURN + CALL RDMT5(WEIGHT,MAXWTS,LENGTH,CUTOFF,SCMAX,IDEV,IOK,IDM,KBOUT, + +IPROB) + END +C********************************************************************* + SUBROUTINE RDMT5(WEIGHT,MAXWTS,LENGTH,CUTOFF,YMAX,IDEV,IOK,IDM, + +KBOUT,IPROB) +C AUTHOR: RODGER STADEN + PARAMETER (MAXLEN = 120, MAXCHR = 5) + REAL WEIGHT(MAXWTS) + INTEGER TOT(MAXLEN),SUM(MAXCHR,MAXLEN) + LENGTH=MAXLEN + CALL RDWMT(TOT,SUM,MIDDLE,LENGTH,MAXLEN,CUTOFF,YMAX,IDEV, + + IOK,IDM,KBOUT,IPROB) + IF(IOK.NE.0)THEN + WRITE(KBOUT,*)' Error in weight matrix, option left' + RETURN + END IF + CALL GETW(TOT,SUM,WEIGHT,LENGTH,IDM,MAXLEN) +C NOW COMPLEMENT THE WEIGHT MATRIX + CALL COMPWM(WEIGHT,LENGTH,MAXLEN,IDM) + END +C********************************************************************* + SUBROUTINE GETMT6(RANGES,RANGEL,LENGTH,CUTOFF, + +KBIN,KBOUT,JDEV,IOK, + + IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + INTEGER RANGES,RANGEL +C GETS DETAILS FOR MOTIF CLASS 6 + IF(JDEV.EQ.KBIN)THEN + IOK = 1 + MININ = 1 + MAXIN = 60 + LENGTH = 6 + CALL GETINT(MININ,MAXIN,LENGTH,'Stem length',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + LENGTH = IVAL + MININ = -LENGTH + MAXIN = 60 + ID = 0 + CALL GETINT(MININ,MAXIN,ID,'Minimum loop length', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + ID = IVAL + MININ = ID + MAXIN = 60 + IR = ID + CALL GETINT(MININ,MAXIN,IR,'Maximum loop length', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IR = IVAL + SMININ = 1 + SMAXIN = 2 * LENGTH + CUTOFF = SMAXIN + CALL GETRL(SMININ,SMAXIN,CUTOFF,'Minimum score', + + VALUE,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CUTOFF = VALUE + ELSE + READ(JDEV,1001,ERR=901)LENGTH + IF(LENGTH.LT.1)RETURN + READ(JDEV,1001,ERR=901)ID +1001 FORMAT(I7) +C IF(ID.LT.0)GO TO 220 + READ(JDEV,1001,ERR=901)IR +C COMMENT NEXT LINE TO ALLOW PALLINDROMES +C IF(IR.LT.0)GO TO 230 + READ(JDEV,1006,ERR=901)CUTOFF +1006 FORMAT(F10.0) + END IF + RANGES = ID + 2*LENGTH + RANGEL = IR + 2*LENGTH + IOK = 0 + RETURN +901 CONTINUE + IOK = 1 + END +C********************************************************************* + SUBROUTINE GETMT7(STRING,MAXSTR,LENGTH,KBIN,KBOUT,IOK,JDEV,STEP, + + IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + CHARACTER NEW(50),STRING(MAXSTR) + REAL STEP +C GETS DETAILS FOR MOTIF CLASS 1 + IOK = 1 + IF(KBIN.EQ.JDEV)THEN +10 CONTINUE + LENGTH = 0 + CALL GETSTR('String',STRING,NEW,50,LENGTH,KBOUT,KBIN,INFLAG) + IF(LENGTH.LT.1) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + CALL SQCOPY(NEW,STRING,LENGTH) + MININ = 1 + MAXIN = 20 + ISTEP = 3 + CALL GETINT(MININ,MAXIN,ISTEP,'Step',IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + ISTEP = IVAL + ELSE + LENGTH = MAXSTR + CALL ARRFIL(JDEV,STRING,LENGTH,KBOUT) + IF(LENGTH.LT.1)THEN + WRITE(KBOUT,*)'ZERO LENGTH STRING' + RETURN + END IF + READ(JDEV,1002,ERR=901)ISTEP +1002 FORMAT(I6) + END IF + STEP = ISTEP + IF(ISTEP.LT.1)STEP = 3.0 + IOK = 0 + RETURN +901 CONTINUE + IOK = 1 + END + SUBROUTINE GETMT8(RANGES,RANGEL,LENGTH,CUTOFF, + +KBIN,KBOUT,JDEV,IOK, + + IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + INTEGER RANGES,RANGEL +C GETS DETAILS FOR MOTIF CLASS 8 + IF(JDEV.EQ.KBIN)THEN + IOK = 1 + MININ = 1 + MAXIN = 60 + LENGTH = 6 + CALL GETINT(MININ,MAXIN,LENGTH,'Repeat length',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + LENGTH = IVAL + MININ = 0 + MAXIN = 60 + ID = MININ + CALL GETINT(MININ,MAXIN,ID,'Minimum gap', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + ID = IVAL + MININ = ID + MAXIN = MININ + 60 + IR = ID + CALL GETINT(MININ,MAXIN,IR,'Maximum gap', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IR = IVAL + SMININ = 1 + SMAXIN = LENGTH + CUTOFF = SMAXIN + CALL GETRL(SMININ,SMAXIN,CUTOFF,'Minimum score', + + VALUE,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CUTOFF = VALUE + ELSE + READ(JDEV,1001,ERR=901)LENGTH + IF(LENGTH.LT.1)RETURN + READ(JDEV,1001,ERR=901)ID +1001 FORMAT(I7) +C IF(ID.LT.0)GO TO 220 + READ(JDEV,1001,ERR=901)IR + READ(JDEV,1006,ERR=901)CUTOFF +1006 FORMAT(F10.0) + END IF + RANGES = ID + LENGTH + 1 + RANGEL = IR + LENGTH + 1 + IOK = 0 + RETURN +901 CONTINUE + IOK = 1 + END + SUBROUTINE DESSIG( + + KBOUT,STRING,MAXSTR, + + LENGTH,CLASS,RELMOT,RANGES,RANGEL, + + RANGET,RANGEM, + + STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF, + + WTSTR,RELEND,COMBIN,KEYNS,TITLE) + CHARACTER STRING(MAXSTR) + INTEGER LENGTH(NMOT),CLASS(NMOT),RELMOT(NMOT),RELEND(NMOT) + INTEGER RANGES(NMOT),RANGEL(NMOT),RANGET(NMOT),RANGEM(NMOT) + INTEGER WTSTR(NMOT),STRNGS(NMOT) + REAL WEIGHT(MAXWTS),CUTOFF(NMOT) + CHARACTER COMBIN(NMOT) + CHARACTER*(*) KEYNS(NMOT),TITLE +C +C +C DESCRIBE THE SIGNAL +C + WRITE(KBOUT,1000) +1000 FORMAT(/' Pattern description',/) + WRITE(KBOUT,1007)TITLE + 1007 FORMAT(' ',A) + DO 100 I = 1,NMOT + WRITE(KBOUT,1001)I,KEYNS(I),CLASS(I) +1001 FORMAT(' Motif ',I2,' named ',A8,' is of class ',I4) +C + IF(CLASS(I).EQ.1)THEN + WRITE(KBOUT,1002) + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) +1002 FORMAT(' Which is an exact match to the string', + + /,(' ',50A1)) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I)-LENGTH(I)+1,RELEND(I),RELMOT(I) +1008 FORMAT(' and the 5 prime base can take positions', + + I7,' to ',I7,/, + + ' relative to the ',I1,' prime end of motif',I4) + ELSE IF(CLASS(I).EQ.2)THEN + WRITE(KBOUT,1003)CUTOFF(I), + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) +1003 FORMAT(' which is a match of score ',F6.0, + + ' to the string',/,(' ',50A1)) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I)-LENGTH(I)+1,RELEND(I),RELMOT(I) + ELSE IF(CLASS(I).EQ.3)THEN + WRITE(KBOUT,1003)CUTOFF(I), + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I)-LENGTH(I)+1,RELEND(I),RELMOT(I) + ELSE IF(CLASS(I).EQ.4)THEN + WRITE(KBOUT,1004)CUTOFF(I) +1004 FORMAT(' Which is a match to a weight matrix with score', + + F8.3) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I)-LENGTH(I)+1,RELEND(I),RELMOT(I) + ELSE IF(CLASS(I).EQ.5)THEN + WRITE(KBOUT,1011)CUTOFF(I) +1011 FORMAT(' Which is a match to the complement of a', + + ' weight matrix with score', + + F8.3) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I)-LENGTH(I)+1,RELEND(I),RELMOT(I) + ELSE IF(CLASS(I).EQ.6)THEN + WRITE(KBOUT,1005)LENGTH(I),CUTOFF(I) +1005 FORMAT(' Which is a stem-loop structure with stem length',I5, + + ' and score ',F6.0) + WRITE(KBOUT,1006)RANGET(I)-2*LENGTH(I),RANGEM(I)-2*LENGTH(I) +1006 FORMAT(' The loop can have sizes ',I6,' to ',I6) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I),RELEND(I),RELMOT(I) + ELSE IF(CLASS(I).EQ.7)THEN + WRITE(KBOUT,1002) + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) + WRITE(KBOUT,1013)INT(CUTOFF(I)) +1013 FORMAT(' with a step size of',I6) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I)-LENGTH(I)+1,RELEND(I),RELMOT(I) + ELSE IF(CLASS(I).EQ.8)THEN + WRITE(KBOUT,1014)LENGTH(I),CUTOFF(I) +1014 FORMAT(' Which is a repeat with repeat length',I5, + + ' and score ',F6.0) + WRITE(KBOUT,1015)RANGET(I)-LENGTH(I)-1,RANGEM(I)-LENGTH(I)-1 +1015 FORMAT(' The loop-out can have sizes ',I6,' to ',I6) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I),RELEND(I),RELMOT(I) + END IF + IF(COMBIN(I).EQ.'O')WRITE(KBOUT,1010) +1010 FORMAT(' It is orred with the previous motif.') + IF(COMBIN(I).EQ.'N')WRITE(KBOUT,1012) +1012 FORMAT(' It is notted with the previous motif.') + IF((COMBIN(I).EQ.'A').AND.(I.GT.1))WRITE(KBOUT,1009) +1009 FORMAT(' It is anded with the previous motif.') +100 CONTINUE + END +C********************************************************************* + SUBROUTINE SAVSIG( + + IDEV,STRING,MAXSTR, + + LENGTH,CLASS,RELMOT,RANGES,RANGEL, + + RANGET,RANGEM, + + STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF, + + WTSTR,RELEND,NAMSAV,COMBIN,KEYNS,TITLE,KBIN,KBOUT) + CHARACTER STRING(MAXSTR) + INTEGER LENGTH(NMOT),CLASS(NMOT),RELMOT(NMOT),RELEND(NMOT) + INTEGER RANGES(NMOT),RANGEL(NMOT),RANGET(NMOT),RANGEM(NMOT) + INTEGER WTSTR(NMOT),STRNGS(NMOT) + REAL WEIGHT(MAXWTS),CUTOFF(NMOT) + CHARACTER COMBIN(NMOT),SCLASS*10 + CHARACTER*(*) KEYNS(NMOT) + CHARACTER*(*) NAMSAV(NMOT),TITLE +C +C +C SAVE THE SIGNAL +C + 1 CONTINUE + LIN = 0 + CALL GTSTR('Pattern title',' ',TITLE,LIN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(INFLAG.EQ.2) RETURN + WRITE(IDEV,1000)TITLE + 1000 FORMAT(' ',A) + DO 100 I = 1,NMOT + WRITE(SCLASS,1014)CLASS(I) +1014 FORMAT(I10) + DO 10 K = 1,10 + IF(SCLASS(K:K).NE.' ')THEN + J = 0 + DO 5 K1 = K,10 + J = J+1 + SCLASS(J:J) = SCLASS(K:K) + SCLASS(K:K) = ' ' +5 CONTINUE + END IF +10 CONTINUE + WRITE(IDEV,1001)COMBIN(I),SCLASS,KEYNS(I) +1001 FORMAT(' ',A,A,' ',A8,' Class ') + IF((I.NE.1).AND.(COMBIN(I).NE.'O'))THEN + WRITE(IDEV,1008)RELMOT(I) +1008 FORMAT(I7,' Relative motif') + IF(CLASS(RELMOT(I)).EQ.6)WRITE(IDEV,1007)RELEND(I) +1007 FORMAT(I7,' Relative end') +1003 FORMAT(I7,' Relative start position') +1004 FORMAT(I7,' Number of extra positions') + WRITE(IDEV,1003)RANGES(I)+1 + WRITE(IDEV,1004)RANGEL(I) + END IF + IF(CLASS(I).EQ.1)THEN + WRITE(IDEV,1002) + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) +1002 FORMAT((' ',50A1)) + WRITE(IDEV,1006) +1006 FORMAT(' @ End of string') + ELSE IF(CLASS(I).EQ.2)THEN + WRITE(IDEV,1002) + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) + WRITE(IDEV,1006) + WRITE(IDEV,1009)CUTOFF(I) + ELSE IF(CLASS(I).EQ.3)THEN + WRITE(IDEV,1002) + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) + WRITE(IDEV,1006) + WRITE(IDEV,1009)CUTOFF(I) + ELSE IF(CLASS(I).EQ.4)THEN + WRITE(IDEV,1005)NAMSAV(I) +1005 FORMAT(A) + ELSE IF(CLASS(I).EQ.5)THEN + WRITE(IDEV,1005)NAMSAV(I) + ELSE IF(CLASS(I).EQ.6)THEN + WRITE(IDEV,1010)LENGTH(I) +1010 FORMAT(I7,' Length') + WRITE(IDEV,1012)RANGET(I)-2*LENGTH(I) + WRITE(IDEV,1013)RANGEM(I)-2*LENGTH(I) +1012 FORMAT(I7,' Minimum loop') +1013 FORMAT(I7,' Maximum loop') + WRITE(IDEV,1009)CUTOFF(I) +1009 FORMAT(F10.5,' Cutoff') + ELSE IF(CLASS(I).EQ.7)THEN + WRITE(IDEV,1002) + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) + WRITE(IDEV,1006) + WRITE(IDEV,1015)INT(CUTOFF(I)) +1015 FORMAT(I7,' Step size') + ELSE IF(CLASS(I).EQ.8)THEN + WRITE(IDEV,1010)LENGTH(I) + WRITE(IDEV,1012)RANGET(I)-LENGTH(I)-1 + WRITE(IDEV,1013)RANGEM(I)-LENGTH(I)-1 + WRITE(IDEV,1009)CUTOFF(I) + END IF +100 CONTINUE + END + SUBROUTINE FORWAD(CLASS,LENGTH,CUTOFF,NMOT, + +MOTIF,ICLASS,ILEN,CUT,IWT,RELMOT,START,IEND, + +RANGES,RANGEL,STRNGS,ISTRST,WTSTR,IDSEQ,IENTRY, + +RANGET,RANGEM,START2,IEND2,MATCHQ,RELEND,MATCHP,IDSPLY, + +COMBIN,COMB) + INTEGER CLASS(NMOT),LENGTH(NMOT),WTSTR(NMOT) + INTEGER STRNGS(NMOT),START(NMOT),IEND(NMOT),IENTRY(NMOT) + INTEGER RELMOT(NMOT),RANGES(NMOT),RANGEL(NMOT) + INTEGER RANGET(NMOT),RANGEM(NMOT),START2(NMOT),IEND2(NMOT) + INTEGER MATCHQ(NMOT),RELEND(NMOT),MATCHP(NMOT) + REAL CUTOFF(NMOT) + CHARACTER COMBIN(NMOT),COMB +CDEBUG +C WRITE(*,*)'IN FORWAD FOR MOTIF',MOTIF +C ROUTINE TO MOVE FORWARDS ONE MOTIF +C FIRST IT UPDATES THE RANGES FOR ALL THE MOTIFS THAT DEPEND +C ON THE CURRENT MOTIF +C IT POINTS TO THE NEXT MOTIF AND GETS ALL THE REQUIRED VARIABLES +C WE MUST FIRST LOOK BACK THRU THE LIST UNTIL WE FIND THE FIRST +C NON ORED MOTIF. THEN WE MUST SET THE RELATIVE POSITIONS FOR ALL +C THOSE MOTIFS THAT DEPEND ON THIS SET OF ORED MOTIFS. THE RELATIVE +C POSITIONS MUST BE SET RELATIVE TO THE POSITION OF THE MATCH FOUND +C FOR THE CURRENT MOTIF EVEN IF IT IS NOT THE FIRST IN THE LIST OF ORS +C THIS IS BECAUSE RELATIVE POSITIONS CAN ONLY BE DEFINED RELATIVE TO +C THE FIRST OF A SET OF ORS, BUT IF A MATCH IS FOUND FOR ANY OF THE OTHERS +C IN THE SET THEN ITS POSITION DEFINES THE RANGE. +C +C +C +C IF THIS IS THE LAST MOTIF RETURN TO DISPLAY THE MATCH + IF(MOTIF.EQ.NMOT)THEN + IDSPLY = 1 + RETURN + END IF +C +C NOT THE LAST +C + IDSPLY = 0 +C +C IF THIS IS A NOTTED MOTIF THEN IT WILL NOT BE ORED OR HAVE ANY +C OTHER MOTIFS RELATIVE TO IT, SO DEAL WITH IT HERE +C + IF(COMB.EQ.'N')THEN + MOTIF = MOTIF + 1 + ICLASS = CLASS(MOTIF) + ILEN = LENGTH(MOTIF) + CUT = CUTOFF(MOTIF) + IWT = WTSTR(MOTIF) + ISTRST = STRNGS(MOTIF) + COMB = COMBIN(MOTIF) + RETURN + END IF +C +C +C NOW LOOK FOR THE FIRST NON ORRED CLASS FROM HERE BACK +C + NOTIF = MOTIF +10 CONTINUE +C + IF(COMBIN(NOTIF).EQ.'O')THEN + NOTIF = NOTIF - 1 + GO TO 10 + END IF +C +C NOTIF IS A NON-ORED MOTIF, OR THE FIRST OF A LIST OF ORS +C SO SET RELATIVE POSITIONS FOR ALL THOSE THAT DEPEND ON IT +C + DO 20 I = MOTIF+1,NMOT + JMOT = I + IF(RELMOT(JMOT).EQ.NOTIF)THEN + IF(CLASS(NOTIF).EQ.6)THEN + IF(RELEND(JMOT).EQ.3) START(JMOT) = MATCHQ(MOTIF) + + + RANGES(JMOT) + IF(RELEND(JMOT).EQ.5) START(JMOT) = MATCHP(MOTIF) + + + RANGES(JMOT) + ELSE + START(JMOT) = START(MOTIF) + RANGES(JMOT) - 1 + END IF +C NEXT LINE FOR RAH BUG + START(JMOT) = MAX(START(JMOT),1) + IEND(JMOT) = START(JMOT) + RANGEL(JMOT) - 1 + IF(IEND(JMOT).GT.IDSEQ)IEND(JMOT)=IDSEQ +C SET FLAG TO SAY THAT FOR STEMS WE DO NOT HAVE TO CONTINUE A PREVIOUS +C 5' STEM START POSITION (IE TRY ALL ITS REMAINING LOOPS) + IENTRY(JMOT) = 0 + END IF +C +20 CONTINUE +C +C +C OK WEVE FOUND THE FIRST NON-ORRED CLASS AND RESET RANGES +C RANGES ACCORDINGLY. NOW WE HAVE TO GO FORARDS THRU THE LIST UNTIL +C WE FIND THE NEXT NON-ORRED CLASS. IF WE SET THIS TO BE THE +C CURRENT MOTIF WE WILL HAVE MOVED FORWARDS. WE MUST BE CAREFUL THAT +C WE DONT FALL OFF THE END OF THE LIST! +C + JMOT = MOTIF +30 CONTINUE + JMOT = JMOT + 1 + IF(JMOT.GT.NMOT)THEN + IDSPLY = 1 + RETURN + END IF + IF(COMBIN(JMOT).EQ.'O')GO TO 30 + MOTIF = JMOT + ICLASS = CLASS(MOTIF) + ILEN = LENGTH(MOTIF) + CUT = CUTOFF(MOTIF) + IWT = WTSTR(MOTIF) + ISTRST = STRNGS(MOTIF) + COMB = COMBIN(MOTIF) +CDEBUG +C DO 1000 I = 1,NMOT +C WRITE(*,*)'START',START(I) +C1000 CONTINUE + END +C********************************************************************* + SUBROUTINE BAKSID(CLASS,LENGTH,CUTOFF,STRNGS,NMOT, + +MOTIF,ICLASS,ILEN,CUT,IWT,ISTRST,WTSTR, + +RELMOT,START,IEND,MATCHQ,RANGES,RANGEL,RELEND,IRET,MATCHP, + +COMBIN,COMB) + INTEGER CLASS(NMOT),LENGTH(NMOT),WTSTR(NMOT) + INTEGER STRNGS(NMOT),RELMOT(NMOT),START(NMOT),IEND(NMOT) + INTEGER MATCHQ(NMOT),RANGES(NMOT),RANGEL(NMOT),RELEND(NMOT) + INTEGER MATCHP(NMOT) + REAL CUTOFF(NMOT) + CHARACTER COMBIN(NMOT),COMB +CDEBUG +C WRITE(*,*)'IN BAKSID FOR MOTIF',MOTIF +C ROUTINE TO MOVE BAKWARDS OR SIDEWAYS +C IE WE COME HERE AFTER FAILING TO FIND A MATCH FOR THE PREVIOUS MOTIF +C WE CHECK IF WE CAN MOVE SIDEWAYS BY LOOKING TO SEE IF THE NEXT MOTIF +C IN THE LIST IS ORRED. +C IF IT IS WE LEAVE THE CURRENT MOTIFS POSITION UNCHANGED SO THAT IT +C IS READY TO SIGNIFIY TO A SUBSEQUENT MOVE BACKWARDS THAT ALL ITS +C POSITIONS HAVE BEEN TRIED FOR THE MATCHES FOUND FURTHER UP THE LIST. +C +C IF THE NEXT MOTIF IN THE LIST IS NOT ORED THEN WE MUST MOVE BACKWARDS. +C BEFORE DOING SO WE MUST RESET THE RANGES FOR ALL THE MOTIFS IN THE +C CURRENT SET OF ORS. THEY MUST BE RESET TO THEIR ORIGINAL FULL RANGE +C BECAUSE WE ARE TRYING TO FIND ALL POSSIBLE COMBINATIONS OF MATCH AND +C WHEN WE GO BACK WE MIGHT NOT GO BACK AS FAR AS THE MOTIF THE CURRENT +C SET DEPEND ON, AND SO THEY WILL NOT BE RESET COMING FORWARDS. SO IF WE +C FIND ANOTHER MATCH TO A MOTIF IN A PREVIOUS SET WE MUST ALLOW THE SAME +C MATCHES TO BE REFOUND IN THE CURRENT SET BECAUSE IT GIVES A DIFFERENT +C COMBINATION TO THOSE ALREADY FOUND. TO FIND THE ONES TO RESET WE MUST +C MOVE BACK THRU THE LIST UNTIL A NON-ORRED CLASS IS FOUND: THIS +C IS THE LAST ONE TO RESET. +C HAVING DONE THIS, TO MOVE BACK WE MUST LOOK BACK FROM THE LAST ONE +C RESET, UNTIL WE FIND THE FIRST NON-ORRED CLASS. THIS WILL +C BE AN ANDED OR NOTTED MOTIF OR THE FIRST IN A LIST OF ORS. +C WE MUST ALWAYS GO BACK +C TO THE BEGINNING OF A LIST OF ORS. IF THEY HAVE NO FURTHER POSITIONS TO +C TRY WE CAN THEN GO SIDEWAYS UNTIL EITHER ONE WITH SOME PLACES TO TRY +C IS FOUND, OR WE HAVE TO GIVE UP AND GO BACKWARDS. +C MOTIF 1 AND ANY MOTIFS ORED WITH IT ARE SPECIAL CASES FOR THE ROUTINES +C BECAUSE THEIR RANGES MUST NOT BE RESET. THEIR SPECIALNESS IS SIGNIFIED +C BY THEIR HAVING A ZERO RELATIVE MOTIF NUMBER. ALSO IF WE TRY TO GO BACK +C FOR MOTIF 1, WE MUST HAVE FINISHED TRYING ALL POSSIBLE COMBINATIONS, +C SO WE ESCAPE. +C +C +C +C RESET ITS MATCH POSITION TO ZERO AS A FLAG TO THE DISPLAY ROUTINES +C +C + MATCHP(MOTIF) = 0 +C +C + IF(MOTIF.LT.NMOT)THEN + IF(COMBIN(MOTIF+1).EQ.'O')THEN +C +C MOVE SIDEWAYS +C + MOTIF = MOTIF + 1 + ICLASS = CLASS(MOTIF) + ILEN = LENGTH(MOTIF) + CUT = CUTOFF(MOTIF) + IWT = WTSTR(MOTIF) + ISTRST = STRNGS(MOTIF) + MATCHP(MOTIF) = 0 + COMB = COMBIN(MOTIF) + RETURN + END IF + END IF +C +C +C +C WE MUST MOVE BACKWARDS. RESET RANGE FOR CURRENT MOTIF IF IT IS NOT +C NOTTED +C +C + IF(COMB.NE.'N')THEN +C +C + IREL = RELMOT(MOTIF) + IF(IREL.NE.0)THEN +C CHANGE ON NEXT LINE MADE 30-07-7 +C IF((CLASS(MOTIF).EQ.6).AND.(RELEND(MOTIF).EQ.3)) +C + START(MOTIF) = MATCHQ(IREL) + RANGES(MOTIF) + IF(CLASS(IREL).EQ.6)THEN + IF(RELEND(MOTIF).EQ.3) + + START(MOTIF) = MATCHQ(IREL) + RANGES(MOTIF) + IF(RELEND(MOTIF).EQ.5) + + START(MOTIF) = MATCHP(IREL) + RANGES(MOTIF) + ELSE + START(MOTIF) = START(IREL) + RANGES(MOTIF) + END IF +C NEXT LINE FOR RAH BUG + START(MOTIF) = MAX(START(MOTIF),1) + IEND(MOTIF) = START(MOTIF) + RANGEL(MOTIF) - 1 + END IF +C +C +C +10 CONTINUE +C +C + IF(MOTIF.EQ.1)THEN + IRET = 1 + RETURN + END IF + IF(COMBIN(MOTIF).EQ.'O')THEN + MOTIF = MOTIF - 1 + MATCHP(MOTIF) = 0 + IREL = RELMOT(MOTIF) + IF(IREL.NE.0)THEN +C CHANGE ON NEXT LINE MADE 30-07-87 +C IF((CLASS(MOTIF).EQ.6).AND.(RELEND(MOTIF).EQ.3)) +C + START(MOTIF) = MATCHQ(IREL) + RANGES(MOTIF) + IF(CLASS(IREL).EQ.6)THEN + IF(RELEND(MOTIF).EQ.3) + + START(MOTIF) = MATCHQ(IREL) + RANGES(MOTIF) + IF(RELEND(MOTIF).EQ.5) + + START(MOTIF) = MATCHP(IREL) + RANGES(MOTIF) + ELSE + START(MOTIF) = START(IREL) + RANGES(MOTIF) + END IF +C NEXT LINE FOR RAH BUG + START(MOTIF) = MAX(START(MOTIF),1) + IEND(MOTIF) = START(MOTIF) + RANGEL(MOTIF) - 1 + END IF + GO TO 10 + END IF +C + END IF +C +C IF WE GET HERE WE HAVE FOUND A NON-ORRED CLASS AND IF THE MOTIF WE CAME +C IN WITH WAS NOT NOTTED +C WE HAVE RESET ITS RANGE AND +C ALL THOSE UP THE LIST THAT ARE ORRED, AND THE CURRENT ONE +C NOW WE MUST FIND THE TOP OF THE NEXT LIST SIGNIFIED BY AN ANDED +C CLASS +C +20 CONTINUE + MOTIF = MOTIF - 1 + IF(COMBIN(MOTIF).NE.'A')GO TO 20 +C IF(COMBIN(MOTIF).EQ.'O')GO TO 20 + ICLASS = CLASS(MOTIF) + ILEN = LENGTH(MOTIF) + CUT = CUTOFF(MOTIF) + IWT = WTSTR(MOTIF) + ISTRST = STRNGS(MOTIF) + COMB = COMBIN(MOTIF) +CDEBUG +C DO 1000 I = 1,NMOT +C WRITE(*,*)'START',START(I) +C1000 CONTINUE + END + SUBROUTINE MOTIF1(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND, + +MATCHP,MATCHS,IFOUND,CUT,JOB) + CHARACTER SEQ(IDIM1),STRING(IDIM2) + REAL MATCHS + IFOUND = 0 + ISTEP = 1 + IF(JOB.EQ.1)ISTEP = CUT + IF(ISTART.LT.1)ISTART=1 + L1 = IEND-ISTART+1 + IF(ISTART.GT.IDIM1)RETURN + IF(L1.LT.IDIM2)RETURN + CALL FIND6(SEQ(ISTART),L1,STRING,IDIM2,ISTEP,IFOUND) + IF(IFOUND.EQ.0)RETURN +C SAVE MATCH POSITION + MATCHP = ISTART+IFOUND-1 + MATCHS = IDIM2 + RETURN + END +C********************************************************************* + SUBROUTINE MOTIF2(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND,CUTOFF, + +MATCHP,MATCHS,IFOUND) + CHARACTER SEQ(IDIM1),STRING(IDIM2) + REAL MATCHS + IFOUND = 0 + IF(ISTART.LT.1)ISTART=1 + IF(ISTART.GT.IDIM1)RETURN + CALL SQFIT4(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND,CUTOFF,MATCHS, + +IFOUND) + IF(IFOUND.EQ.0)RETURN +C SAVE MATCH POSITION + MATCHP = IFOUND + RETURN + END + SUBROUTINE GETCLS(JDEV,KBOUT,ICLASS,TCLASS,KEYNAM) + CHARACTER TCLASS,STRING*21,NUMBER*10,STRNG2*10,KEYNAM*(*) + SAVE NUMBER + DATA NUMBER/'0987654321'/ +C WRITE(*,*)'JDEV,KBOUT,ICLASS,TCLASS',JDEV,KBOUT,ICLASS,TCLASS + ICLASS = 0 +10 CONTINUE + STRING = ' ' + READ(JDEV,1000,ERR=10,END=70)STRING +1000 FORMAT(A) + KEYNAM = STRING(14:21) + DO 20 I = 10,1,-1 + K = I + IF(STRING(I:I).NE.' ')GO TO 21 +20 CONTINUE + RETURN +21 CONTINUE + L = 1 + DO 30 I = 1,K + L = I + IF(STRING(I:I).NE.' ')GO TO 31 +30 CONTINUE +31 CONTINUE + TCLASS = STRING(L:L) + DO 40 I = 1,10 + IF(TCLASS.EQ.NUMBER(I:I))THEN + TCLASS = 'A' + GO TO 41 + END IF +40 CONTINUE + IF((TCLASS.NE.'O').AND.(TCLASS.NE.'N').AND.(TCLASS.NE.'A') + +.AND.(TCLASS.NE.'-'))THEN + ICLASS = 99999 + RETURN + END IF + L = L + 1 +41 CONTINUE + J = 11 + STRNG2 = ' ' + DO 50 I = K,L,-1 + J = J-1 + STRNG2(J:J) = STRING(I:I) +50 CONTINUE + READ(STRNG2,1001,ERR=60)ICLASS +1001 FORMAT(I10) + IF(TCLASS.EQ.'-')ICLASS=-1*ICLASS + RETURN +60 CONTINUE + ICLASS = 99999 + RETURN +70 CONTINUE + END +C********************************************************************* +C********************************************************************* + SUBROUTINE SQFIT4(SEQ,IDIM1,STRING,IDIM2, + 1IS,IE,MINSC,MATCHS,IFOUND) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2) + REAL MATCHS,MINSC + INTEGER IUBM1 + EXTERNAL IUBM1 + MINSCR = MINSC + IDIF=(IE-IS+2)-IDIM2 + IPSEQ=IS + DO 200 I=1,IDIF + NTOT=0 + IP=IPSEQ + DO 100 J=1,IDIM2 + NTOT = NTOT + IUBM1(STRING(J),SEQ(IP)) + IP=IP+1 +100 CONTINUE +C END OF COUNTING FOR THIS POSITION.IS TOTAL HIGH ENOUGH? + IF(NTOT.GE.MINSCR)THEN + MATCHS = NTOT + IFOUND = IP-IDIM2 + RETURN + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + IFOUND = 0 + RETURN + END + SUBROUTINE GETP(ICLASS,STRING,LENGTH,IDM, + +MAT1,IDMAT1,MAT2,IDMAT2,MAT3,IDMAT3,MAT4,IDMAT4,WEIGHT) +C MAT1 SIMPLE IDENTITY +C MAT2 IUB SCORES 0-1 +C MAT3 IUB SCORES 0-36 +C MAT4 INVERTED REPEAT + PARAMETER ( + + MAXCHR=17, + + MAXSIG=120) + INTEGER MAT1(IDMAT1,IDMAT1),MAT2(IDMAT2,IDMAT2) + INTEGER MAT3(IDMAT3,IDMAT3),MAT4(IDMAT4,IDMAT4) + INTEGER IWT(MAXCHR,MAXSIG) + REAL WEIGHT(IDM,LENGTH),FB(MAXCHR),F(MAXCHR) + CHARACTER STRING(LENGTH) + COMMON /COMPC/COMP(MAXCHR) + DO 10 I = 1,MAXCHR + F(I) = COMP(I) +10 CONTINUE + IF(ICLASS.EQ.1)THEN + CALL GETP1(STRING,LENGTH,IDMAT3,MAT2,IDMAT2,IWT,F) + RETURN + END IF + IF(ICLASS.EQ.2)THEN + CALL GETP1(STRING,LENGTH,IDMAT3,MAT2,IDMAT2,IWT,F) + RETURN + END IF + IF(ICLASS.EQ.3)THEN + CALL GETP3(STRING,LENGTH,IDMAT3,MAT3,IWT,IDM,F) + RETURN + END IF + IF(ICLASS.EQ.4)THEN + CALL GETP4(WEIGHT,IWT,IDM,LENGTH,F) + RETURN + END IF + IF(ICLASS.EQ.5)THEN + CALL GETP4(WEIGHT,IWT,IDM,LENGTH,F) + RETURN + END IF + IF(ICLASS.EQ.6)THEN + CALL GETP8(MAT4,IDMAT4,IWT,IDM,LENGTH,F,FB) + RETURN + END IF + IF(ICLASS.EQ.7)THEN + CALL GETP1(STRING,LENGTH,IDMAT3,MAT2,IDMAT2,IWT,F) + RETURN + END IF + IF(ICLASS.EQ.8)THEN + CALL GETP8(MAT1,IDMAT1,IWT,IDM,LENGTH,F,FB) + RETURN + END IF + END + SUBROUTINE WTGEN(IWT,MAXCHR,LENGTH,F,MAXS,SMIN,SCALE,JOB) + PARAMETER (MAXPOL = 4000) + INTEGER IWT(MAXCHR,LENGTH) + REAL F(MAXCHR) + COMMON /POLY/POLYA(0:MAXPOL),POLYB(0:MAXPOL),POLYC(0:MAXPOL), + +CMIN,CSCALE,LENTHC + SAVE /POLY/ + CMIN = SMIN + CSCALE = SCALE + LENTHC = LENGTH + DO 10 I = 0, MAXPOL + POLYA(I) = 0.0 +10 CONTINUE + DO 400 I = 1,MAXCHR + K = IWT(I,1) + POLYA(K) = POLYA(K) + F(I) +400 CONTINUE + MAXS1 = MAXS + DO 600 J = 2,LENGTH + DO 490 I = 0, MAXPOL + POLYB(I) = 0.0 +490 CONTINUE + DO 500 I = 1,MAXCHR + K = IWT(I,J) + POLYB(K) = POLYB(K) + F(I) +500 CONTINUE + CALL POLMUL(MAXS1,MAXS,MAXSP1) + MAXS1 = MAXSP1 +600 CONTINUE +C IF JOB = 1 WANT DISTRIBUTION ONLY +C JOB = 2 WANT CUMMULATIVE VALUES +C JOB = 3 WANT DISTRIBUTION AND UNSCALED SCORES +C JOB = 4 WANT CUMMULATIVE VALUES AND UNSCALED SCORES + IF((JOB.EQ.2).OR.(JOB.EQ.4))THEN + DO 610 I = 1,MAXS1 + J = MAXS1 - I + POLYA(J) = POLYA(J) + POLYA(J+1) +610 CONTINUE + END IF + IF((JOB.EQ.3).OR.(JOB.EQ.4))THEN + T = SMIN * LENGTH + DO 620 I = 0,MAXS1 + POLYB(I) = (REAL(I)/SCALE) + T +620 CONTINUE + END IF + END + SUBROUTINE SCGEN(IWT,MAXCHR,LENGTH,FA,FB,MAXS, + +SCALE,SMIN,JOB) +C AUTHOR RODGER STADEN +C PROBABILITIES FOR DIRECT AND INVERTED REPEATS + PARAMETER (MAXPOL = 4000) + INTEGER IWT(MAXCHR,MAXCHR) + REAL FA(MAXCHR),FB(MAXCHR) + COMMON /POLY/POLYA(0:MAXPOL),POLYB(0:MAXPOL),POLYC(0:MAXPOL), + +CMIN,CSCALE,LENTHC + SAVE /POLY/ + CMIN = SMIN + CSCALE = SCALE + LENTHC = LENGTH + DO 10 I = 0, MAXPOL + POLYA(I) = 0.0 + POLYB(I) = 0.0 +10 CONTINUE + DO 400 I = 1,MAXCHR + DO 300 J = 1,MAXCHR + K = IWT(I,J) + POLYA(K) = POLYA(K) + FA(I) * FB(J) + POLYB(K) = POLYA(K) +300 CONTINUE +400 CONTINUE + MAXS1 = MAXS + DO 600 J = 2,LENGTH + CALL POLMUL(MAXS1,MAXS,MAXSP1) + MAXS1 = MAXSP1 +600 CONTINUE +C IF JOB = 1 WANT DISTRIBUTION ONLY +C JOB = 2 WANT CUMMULATIVE VALUES +C JOB = 3 WANT DISTRIBUTION AND UNSCALED SCORES +C JOB = 4 WANT CUMMULATIVE VALUES AND UNSCALED SCORES + IF((JOB.EQ.2).OR.(JOB.EQ.4))THEN + DO 610 I = 1,MAXS1 + J = MAXS1 - I + POLYA(J) = POLYA(J) + POLYA(J+1) +610 CONTINUE + END IF + IF((JOB.EQ.3).OR.(JOB.EQ.4))THEN + T = SMIN * LENGTH + DO 620 I = 0,MAXS1 + POLYB(I) = (REAL(I)/SCALE) + T +620 CONTINUE + END IF + END + SUBROUTINE SCGEN1(IWT,MAXCHR,LENGTH,FA,MAXS, + +SCALE,SMIN,JOB,STRING) +C AUTHOR RODGER STADEN +C PROBABILITY FOR STRINGS + CHARACTER STRING(LENGTH) + INTEGER DTONUM + PARAMETER (MAXPOL = 4000) + INTEGER IWT(MAXCHR,MAXCHR) + REAL FA(MAXCHR) + COMMON /POLY/POLYA(0:MAXPOL),POLYB(0:MAXPOL),POLYC(0:MAXPOL), + +CMIN,CSCALE,LENTHC + EXTERNAL DTONUM + SAVE /POLY/ + CMIN = SMIN + CSCALE = SCALE + LENTHC = LENGTH + DO 10 I = 0, MAXPOL + POLYA(I) = 0.0 +10 CONTINUE + K1 = DTONUM(STRING(1)) + DO 400 I = 1,MAXCHR + K = IWT(I,K1) + POLYA(K) = POLYA(K) + FA(I) +400 CONTINUE + MAXS1 = MAXS + DO 600 J = 2,LENGTH + DO 490 I = 0,MAXPOL + POLYB(I) = 0. +490 CONTINUE + K1 = DTONUM(STRING(J)) + DO 500 I = 1,MAXCHR + K = IWT(I,K1) + POLYB(K) = POLYB(K) + FA(I) +500 CONTINUE + CALL POLMUL(MAXS1,MAXS,MAXSP1) + MAXS1 = MAXSP1 +600 CONTINUE +C IF JOB = 1 WANT DISTRIBUTION ONLY +C JOB = 2 WANT CUMMULATIVE VALUES +C JOB = 3 WANT DISTRIBUTION AND UNSCALED SCORES +C JOB = 4 WANT CUMMULATIVE VALUES AND UNSCALED SCORES + IF((JOB.EQ.2).OR.(JOB.EQ.4))THEN + DO 610 I = 1,MAXS1 + J = MAXS1 - I + POLYA(J) = POLYA(J) + POLYA(J+1) +610 CONTINUE + END IF + IF((JOB.EQ.3).OR.(JOB.EQ.4))THEN + T = SMIN * LENGTH + DO 620 I = 0,MAXS1 + POLYB(I) = (REAL(I)/SCALE) + T +620 CONTINUE + END IF + END + SUBROUTINE POLMUL(NA,NB,NC) + PARAMETER (MAXPOL = 4000) + COMMON /POLY/POLYA(0:MAXPOL),POLYB(0:MAXPOL),POLYC(0:MAXPOL), + +CMIN,CSCALE,LENTHC + PARAMETER (ZERO=0.0,SMALL=1E-10) + SAVE /POLY/ +C POLYA, POLYB ARE INPUT POLYNOMIAL COEEFICIENTS +C POLYC IS OUTPUT POLYNOMIAL COEEFICIENTS +C + NC = NA + NB + DO 210 I = 0,MAXPOL + POLYC(I) = ZERO +210 CONTINUE + IF(NC.GT.MAXPOL)RETURN + DO 230 I = 0,NA + DO 220 J = 0,NB + POLYC(I+J) = POLYC(I+J) + POLYA(I) * POLYB(J) +220 CONTINUE +230 CONTINUE + DO 240 I = 0,NC + T = POLYC(I) + IF(T.LT.SMALL) T = ZERO + POLYA(I) = T +240 CONTINUE + END + SUBROUTINE WRTWMT(WEIGHT,IWT,MAXCHR,LENGTH,IDEV,IFLAG) + REAL WEIGHT(MAXCHR,LENGTH) + INTEGER IWT(MAXCHR,LENGTH) + DO 10 I = 1,MAXCHR + IF(IFLAG.EQ.0) + + WRITE(IDEV,1000,ERR=100)(WEIGHT(I,J),J=1,LENGTH) + IF(IFLAG.EQ.1) + + WRITE(IDEV,1001,ERR=100)(IWT(I,J),J=1,LENGTH) +10 CONTINUE +1000 FORMAT(' ',20F5.2) +1001 FORMAT(' ',20I4) +100 CONTINUE + END + SUBROUTINE WTSC(WEIGHT,IWT,MAXCHR,LENGTH,MAXS,SCALE,SMIN) + INTEGER IWT(MAXCHR,LENGTH) + REAL WEIGHT(MAXCHR,LENGTH) + PARAMETER (SMALL=1E-10) + PARAMETER (MAXPOL = 4000) + SMIN = 9999999.9 + SMAX = -99999999.9 + DO 100 J = 1,LENGTH + DO 90 I = 1,MAXCHR + T = WEIGHT(I,J) + SMIN = MIN(SMIN,T) + SMAX = MAX(SMAX,T) +90 CONTINUE +100 CONTINUE + SMAX = SMAX - SMIN + IF(SMAX.EQ.0.0)SMAX = SMALL +C USE LENGTH+1 TO DIVIDE MAXPOL. THIS IS AN ATTEMPT TO AVOID +C THE USE OF NINT GIVING A SET OF SCORES THAT SUM TO TOO +C HIGH A VALUE (I.E GT MAXPOL) + MAXS = MAXPOL / (LENGTH+1) + MAXS = MIN(MAXS,40) + SCALE = REAL(MAXS)/SMAX + DO 200 J = 1,LENGTH + DO 190 I = 1,MAXCHR + K = NINT((WEIGHT(I,J)-SMIN)*SCALE) + IWT(I,J) = K +190 CONTINUE +200 CONTINUE + END + SUBROUTINE GETCMP(SEQ,IDIM,COMP,IDM) + CHARACTER SEQ(IDIM) + REAL COMP(IDM) + INTEGER DTONUM + EXTERNAL DTONUM + DO 10 I = 1,IDM + COMP(I) = 0.0 +10 CONTINUE + DO 20 I = 1,IDIM + J = DTONUM(SEQ(I)) + COMP(J) = COMP(J) + 1. +20 CONTINUE + T = MAX(1,IDIM) + DO 30 I = 1,IDM + COMP(I) = COMP(I) / T +30 CONTINUE + END + SUBROUTINE MATSC(MAT,IDMAT,IWT,MAXCHR,LENGTH,MAXS, + +SMIN,SCALE) + INTEGER IWT(IDMAT,IDMAT) + INTEGER MAT(IDMAT,IDMAT) + PARAMETER (SMALL=1E-10) + PARAMETER (MAXPOL = 4000) + ISMIN = 99999999 + ISMAX = -99999999 + DO 100 J = 1,IDMAT + DO 90 I = 1,IDMAT + K = MAT(I,J) + ISMIN = MIN(ISMIN,K) + ISMAX = MAX(ISMAX,K) +90 CONTINUE +100 CONTINUE +C SMAX = ISMAX - ISMIN +C IF(SMAX.EQ.0.0)SMAX = SMALL +C DO NOTHING HERE - ASSUME VALUES ARE IN RANGE AND JUST COPY +C USE LENGTH+1 TO DIVIDE MAXPOL. THIS IS AN ATTEMPT TO AVOID +C THE USE OF NINT GIVING A SET OF SCORES THAT SUM TO TOO +C HIGH A VALUE ( IE GT MAXPOL) +C MAXS = MAXPOL(LENGTH+1) +C SCALE = REAL(MAXS)/SMAX + DO 200 J = 1,IDMAT + DO 190 I = 1,IDMAT +C K = NINT((REAL(WEIGHT(I,J))-ISMIN)*SCALE) + IWT(I,J) = MAT(I,J) - ISMIN +190 CONTINUE +200 CONTINUE + SCALE = 1.0 + SMIN = ISMIN + MAXS = ISMAX + END + REAL FUNCTION PSCORE(SCORE) + PARAMETER (MAXPOL = 4000) + COMMON /POLY/POLYA(0:MAXPOL),POLYB(0:MAXPOL),POLYC(0:MAXPOL), + +SMIN,SCALE,LENGTH + SAVE /POLY/ +C WANT PROBABILITY OF SCORING AT LEAST SCORE +C CUMMULATIVE PROBABILITIES IN CUMP +C SCALE FACTORS SMIN AND SCALE AND MOTIF LENGTH IS LENGTH + I = NINT((SCORE - SMIN * LENGTH) * SCALE) + PSCORE = -1. + IF((I.GE.0).AND.(I.LE.MAXPOL)) PSCORE = POLYA(I) + END + SUBROUTINE GETP1(STRING,LENGTH,IDMAT3,MAT2,IDMAT2,IWT,F) + INTEGER MAT2(IDMAT2,IDMAT2) + INTEGER IWT(IDMAT2,IDMAT2) + REAL F(IDMAT3) + CHARACTER STRING(LENGTH) + JOB = 2 + CALL MATSC(MAT2,IDMAT2,IWT,IDMAT3,LENGTH,MAXS,SMIN,SCALE) + CALL SCGEN1(IWT,IDMAT2,LENGTH,F,MAXS,SCALE,SMIN,JOB,STRING) + END + SUBROUTINE GETP3(STRING,LENGTH,IDMAT3,MAT3,IWT,IDM,F) + INTEGER MAT3(IDMAT3,IDMAT3) + INTEGER IWT(IDMAT3,IDMAT3) + REAL F(IDMAT3) + CHARACTER STRING(LENGTH) + JOB = 2 + CALL MATSC(MAT3,IDMAT3,IWT,IDM,LENGTH,MAXS,SMIN,SCALE) + CALL SCGEN1(IWT,IDMAT3,LENGTH,F,MAXS,SCALE,SMIN,JOB, + + STRING) + END + SUBROUTINE GETP4(WEIGHT,IWT,IDM,LENGTH,F) + REAL F(IDM) + REAL WEIGHT(IDM,LENGTH) + INTEGER IWT(IDM,LENGTH) + JOB = 2 + CALL WTSC(WEIGHT,IWT,IDM,LENGTH,MAXS,SCALE,SMIN) + CALL WTGEN(IWT,IDM,LENGTH,F,MAXS,SMIN,SCALE,JOB) + END + SUBROUTINE GETP8(MAT1,IDMAT1,IWT,IDM,LENGTH,F,FB) + REAL F(IDM),FB(IDM) + INTEGER MAT1(IDMAT1,IDMAT1) + INTEGER IWT(IDM,LENGTH) + DO 30 I = 1,IDM + FB(I) = F(I) +30 CONTINUE + JOB = 2 + CALL MATSC(MAT1,IDMAT1,IWT,IDM,LENGTH,MAXS,SMIN,SCALE) + CALL SCGEN(IWT,IDMAT1,LENGTH,F,FB,MAXS,SCALE,SMIN,JOB) + END + SUBROUTINE WRTSCR(SCMIN,PROB,KBOUT) + WRITE(KBOUT,1000)SCMIN,PROB +1000 FORMAT(' Probability of score ',F10.4,' =',E10.3) + END + SUBROUTINE EFTOUT(FTNAME,I1,I2,IDEV) + CHARACTER LINOUT*80,FTNAME*(*) + EXTERNAL ITOSL +C produces ft lines from a pair of positions + LINOUT = 'FT ' + LINOUT(6:80) = FTNAME + L = 22 + J = ITOSL(LINOUT(L:),I1) + IF(J.EQ.0) GO TO 100 + L = L + J + LINOUT(L:L+1) = '..' + L = L + 2 + J = ITOSL(LINOUT(L:),I2) + IF(J.EQ.0) GO TO 100 + WRITE(IDEV,1000,ERR=100)LINOUT + 1000 FORMAT(A) + RETURN +100 CONTINUE + WRITE(*,*)'Error in EFTOUT' + END diff --git a/src/staden/patternp.f b/src/staden/patternp.f new file mode 100644 index 0000000..411c571 --- /dev/null +++ b/src/staden/patternp.f @@ -0,0 +1,1027 @@ +C 22-7-91 fixed bug in dsplay: graphics did not work +C 2-3-92 set filnam = ' ' for some calls to openf1 + SUBROUTINE PATTEN(SEQ,IDSEQ,STRING,MAXSTR, + +LENGTH,CLASS,RELMOT,RANGES,RANGEL,RANGET,RANGEM,IENTRY, + +START2,IEND2,WTSTR,START,IEND,MATCHQ,RELEND,MATCHP, + +STRNGS,LAST5,LAST3S,LAST3E,MATCHS,CUTOFF,WEIGHT,FILNAM, + +MAXMOT,MAXWTS,MATRIX, + +IDEV1,IDEV2,IDEV3, + +MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,KSTART,KBIN,KBOUT, + +IDEV4,IDM,COMBIN,MAT1,NAMSAV,KEYNS,CHRSET, + +IHELPS,IHELPE,HELPF,IDEVH,FOFNAM,IDEVFF) + INTEGER LENGTH(MAXMOT),CLASS(MAXMOT),RELMOT(MAXMOT) + INTEGER RANGES(MAXMOT),RANGEL(MAXMOT) + INTEGER RANGET(MAXMOT),RANGEM(MAXMOT),IENTRY(MAXMOT) + INTEGER START2(MAXMOT),IEND2(MAXMOT) + CHARACTER SEQ(IDSEQ),STRING(MAXSTR) + INTEGER WTSTR(MAXMOT),START(MAXMOT),IEND(MAXMOT) + INTEGER MATCHQ(MAXMOT),RELEND(MAXMOT) + INTEGER MATCHP(MAXMOT),STRNGS(MAXMOT) + INTEGER LAST5(MAXMOT),LAST3S(MAXMOT),LAST3E(MAXMOT) + INTEGER MAT1(IDM,IDM),MATRIX(IDM,IDM) + REAL WEIGHT(MAXWTS),CUTOFF(MAXMOT),MATCHS(MAXMOT) + REAL MINSCR,MAXSCR + CHARACTER FILNAM*(*),HELPF*(*),FOFNAM*(*),TITLE*80 + CHARACTER COMBIN(MAXMOT),CHRSET(IDM) + CHARACTER*(*) NAMSAV(MAXMOT),KEYNS(MAXMOT) + PARAMETER (MAXCLS = 6) + PARAMETER (SMALL=1.0E-5) + PARAMETER (MAXPRM = 30) + CHARACTER PROMPT(4)*(MAXPRM) + WRITE(KBOUT,*)' Pattern searcher' + JDEV = KBIN + PROMPT(1) = 'Use keyboard' + PROMPT(2) = 'Use pattern file' + PROMPT(3) = 'Use file of pattern file names' + IN = 1 + CALL RADION('Select pattern definition mode',PROMPT,3,IN, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IN.LT.1) RETURN + IF(IN.EQ.2)THEN + FILNAM = ' ' + CALL OPENF1(IDEV3,FILNAM,0,IOK,KBIN,KBOUT, + + 'Pattern definition file', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + JDEV = IDEV3 + ELSE IF(IN.EQ.3) THEN + FOFNAM = ' ' + CALL OPENF1(IDEVFF,FOFNAM,0,IOK,KBIN,KBOUT, + + 'File of pattern file names', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + JDEV = IDEV3 + END IF + IOPT = 1 + PROMPT(1) = 'Motif by motif' + PROMPT(2) = 'Inclusive' + PROMPT(3) = 'Graphical' + PROMPT(4) = 'EMBL feature table' + CALL RADION('Select results display mode',PROMPT,4,IOPT, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOPT.LT.1) GO TO 201 + IF((IN.EQ.3).AND.(IOPT.EQ.3)) IOPT = 2 + PMINC = 1. + CUTSCR = -99999. + NPAT = 0 + IPROB = 0 + NTOTAL = 0 + JTOTAL = 0 + CALL SETCMP(SEQ,IDSEQ,IDM) + IF(IN.EQ.3) THEN + IPROB = 1 + CALL BUSY(KBOUT) + END IF +10 CONTINUE + IF(IN.EQ.3) THEN + READ(IDEVFF,1000,ERR=100,END=200)FOFNAM + CALL OPENRS(IDEV3,FOFNAM,IOK,LRECL,2) + IF(IOK.NE.0) GO TO 200 + NPAT = NPAT + 1 + END IF +1000 FORMAT(A) + CALL FILLI(LENGTH,MAXMOT,0) + CALL FILLI(CLASS,MAXMOT,0) + CALL FILLI(RELMOT,MAXMOT,0) + CALL FILLI(RANGES,MAXMOT,0) + CALL FILLI(RANGEL,MAXMOT,0) + CALL FILLI(RANGET,MAXMOT,0) + CALL FILLI(RANGEM,MAXMOT,0) + CALL FILLI(IENTRY,MAXMOT,0) + CALL FILLI(START2,MAXMOT,0) + CALL FILLI(IEND2,MAXMOT,0) + CALL FILLI(WTSTR,MAXMOT,0) + CALL FILLI(START,MAXMOT,0) + CALL FILLI(IEND,MAXMOT,0) + CALL FILLI(MATCHQ,MAXMOT,0) + CALL FILLI(RELEND,MAXMOT,0) + CALL FILLI(MATCHP,MAXMOT,0) + CALL FILLI(STRNGS,MAXMOT,0) + CALL FILLI(LAST5,MAXMOT,0) + CALL FILLI(LAST3S,MAXMOT,0) + CALL FILLI(LAST3E,MAXMOT,0) + CALL FILLR(CUTOFF,MAXMOT,0.0) + CALL FILLR(MATCHS,MAXMOT,0.0) + CALL FILLR(WEIGHT,MAXWTS,0.0) + CALL FILLC(COMBIN,MAXMOT,'A') + DO 1 I = 1,MAXMOT + NAMSAV(I) = 'FILENOTUSED' +C WRITE(*,*)NAMSAV(I) +1 CONTINUE + ITOTAL = 0 +C +C +C +C GET MOTIF DEFINITIONS +C +C RETURN STRING LENGTH FOR COMPATIBILITY WITH LIB SEARCH + NSTRNG = MAXSTR + IF(IN.EQ.1) THEN + CALL GETMK(KBIN,KBOUT,STRING,NSTRNG,ISTRNG, + + LENGTH,MAXMOT,CLASS,RELMOT,RANGES,RANGEL, + + RANGET,RANGEM, + + STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF,IDEV2, + + WTSTR,JDEV,IOK,RELEND,IDSEQ,IDEV4,IDM,COMBIN, + + MAXCLS,MATRIX,MAT1, + + PMINT,PMAXT,PROBT,EXPTT,CHRSET, + + IHELPS,IHELPE,HELPF,IDEVH,KEYNS,NAMSAV,FILNAM,TITLE) + ELSE + CALL GETMF(KBIN,KBOUT,STRING,NSTRNG,ISTRNG, + + LENGTH,MAXMOT,CLASS,RELMOT,RANGES,RANGEL, + + RANGET,RANGEM, + + STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF,IDEV2, + + WTSTR,JDEV,IOK,RELEND,IDSEQ,IDEV4,IDM,COMBIN, + + MAXCLS,MATRIX,MAT1, + + PMINT,PMAXT,PROBT,EXPTT,CHRSET, + + IHELPS,IHELPE,HELPF,IDEVH,KEYNS,NAMSAV,FILNAM,IPROB,TITLE) + END IF + IF(IOK.NE.0) GO TO 201 + IF(NMOT.LT.1) GO TO 201 +C +C +C DISPLAY THE SIGNAL DESCRIPTION +C + RANGES(1) = 1 + IF(IN.NE.3) THEN + CALL DESSIG( + + KBOUT,STRING,MAXSTR, + + LENGTH,CLASS,RELMOT,RANGES,RANGEL, + + RANGET,RANGEM, + + STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF, + + WTSTR,RELEND,COMBIN,KEYNS,TITLE) +C +C + WRITE(KBOUT,2003)PROBT +2003 FORMAT(' Probability of finding pattern = ',E10.4) + WRITE(KBOUT,2004)EXPTT +2004 FORMAT(' Expected number of matches = ',E10.4) + CALL GETRL(0.,1.,1.0,'Maximum pattern probability', + + XP,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) GO TO 201 + PMINC = XP + CALL GETRL(-9999.,9999.,-9999.,'Minimum pattern score', + + XP,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) GO TO 201 + CUTSCR = XP + MINSCR = 9999999. + MAXSCR = -9999999. + YMIN = LOG(1.-PMINT) + YMAX = LOG(1.-PMAXT) + YDEL = ABS(YMIN - YMAX) + IF(YDEL.LT.SMALL)THEN + YMAX = YMAX + SMALL + YMIN = YMIN - SMALL + END IF + YDEL = ABS(YMIN - YMAX) * 0.1 + YMIN = YMIN - YDEL + YMAX = YMAX + YDEL + CALL BUSY(KBOUT) + END IF + IF(IOPT.EQ.3)THEN + XMAX = IDSEQ + XMIN = KSTART + CALL VECTOM + CALL FRAME(MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL CLEARV + END IF +C +C DO THE SEARCH +C + CALL SRCSIG(KBIN,KBOUT,WTSTR,LENGTH,CLASS, + +RANGES,RANGEL,START,IEND,RELMOT,MATCHP,STRNGS,WEIGHT, + +MAXWTS,CUTOFF,MATCHS,NMOT,STRING,MAXSTR,SEQ,IDSEQ, + +RANGET,RANGEM,IENTRY,START2,IEND2,MATRIX,MATCHQ,RELEND, + +IDEV1,LAST5,LAST3S,LAST3E,MARGL,MARGR,MARGB,MARGT,ISXMAX, + +ISYMAX,IOPT,XMAX,XMIN,YMAX,YMIN,ITOTAL,KSTART,IDM,COMBIN, + +CUTSCR,MINSCR,MAXSCR,MAT1,PMINT,PMAXT,PROBT,PMINC,KEYNS) + CALL VT100M + IF(IN.EQ.3) THEN + IF(ITOTAL.NE.0) THEN + WRITE(IDEV1,1003)TITLE + 1003 FORMAT(' ',A) + WRITE(IDEV1,1001)FOFNAM,ITOTAL +1001 FORMAT(' ',A,' ',I6,/) + NTOTAL = NTOTAL + ITOTAL + JTOTAL = JTOTAL + 1 + END IF + GO TO 10 + END IF + WRITE(KBOUT,1002)ITOTAL +1002 FORMAT(' Total matches found',I7) + IF((ITOTAL.GT.0).AND.(IN.NE.3))WRITE(KBOUT,1006)MINSCR,MAXSCR +1006 FORMAT(' Minimum and maximum observed scores',2F12.2) + RETURN +100 CONTINUE + WRITE(KBOUT,*)'Error reading file of file names' +200 CONTINUE + WRITE(KBOUT,*)'Of the ',NPAT,' patterns processed' + WRITE(KBOUT,*)JTOTAL,' gave a total of',NTOTAL,' matches' +201 CONTINUE + CLOSE(UNIT=IDEVFF) + END + SUBROUTINE SRCSIG(KBIN,KBOUT,WTSTR,LENGTH,CLASS, + +RANGES,RANGEL,START,IEND,RELMOT,MATCHP,STRNGS,WEIGHT, + +MAXWTS,CUTOFF,MATCHS,NMOT,STRING,MAXSTR,SEQ,IDSEQ, + +RANGET,RANGEM,IENTRY,START2,IEND2,MATRIX,MATCHQ,RELEND, + +IDEVOT,LAST5,LAST3S,LAST3E,MARGL,MARGR,MARGB,MARGT,ISXMAX, + +ISYMAX,IOPT,XMAX,XMIN,YMAX,YMIN,ITOTAL,KSTART,IDM,COMBIN, + +CUTSCR,MINSCR,MAXSCR,MAT1,PMINT,PMAXT,PROBT,PMINC,KEYNS) +C ROUTINE TO SEARCH FOR SIGNALS COMPOSED OF MOTIFS +C WEIGHT = WEIGHTS FOR MATRICES +C CUTOFF = CUTOFF SCORES +C LENGTH = MOTIF LENGTHS +C CLASS = MOTIF CLASS +C COMBIN = LOGICAL COMBINATION A, O, N +C WTSTR = POINTER TO WEIGHT STARTS +C RANGES = RANGES START +C RANGEL = RANGE LENGTH (A DISTANCE MEASURED FROM RANGES) +C RELMOT = MOTIF NUMBER THAT A RANGE IS RELATIVE TO IE THE +C FIRST MOTIF'S RANGE IS RELATIVE TO MOTIF 0, BUT +C ANY OTHER MOTIF MAY HAVE TO BE DEFINED RELATIVE +C TO ANY OTHER. THE MOST COMMON WOULD BE THE FIRST +C MOTIF OR THE LAST ONE SEARCHED FOR. +C RELEND = IS A SPECIAL CASE FLAG FOR STEMS. IT ALLOWS OTHER +C MOTIFS TO HAVE THEIR POSITIONS RELATIVE TO THE 3' SIDE +C OF A STEM. IT IS 5 FOR THE 5 PRIME SIDE, 3 FOR 3' SIDE +C START = RANGE START DURING SEARCH (SOME POSITIONS MAY HAVE +C BEEN TRIED) +C IEND = RANGE END POSITION FOR CURRENT INITIAL START (WHEN IT +C IS RESET DEPENDS ON WHETHER IT IS DEFINED RELATIVE TO +C THE FIRST OR THE PREVIOUS MOTIF. IF IT IS DEFINED RELATIVE +C TO THE FIRST MOTIF IT IS RESET WHEN WE FIND A MATCH FOR THE +C FIRST MOTIF. IF IT IS DEFINED RELATIVE TO THE PREVIOUS MOTIF +C WE MUST RESET WHEN WE MOVE FORWARD ONE MOTIF. I THINK THIS +C CAN BE TAKEN CARE OF BY UPDATING ALL THOSE MOTIFS THAT ARE +C DEFINED TO THE CURRENT MOTIF EVERY TIME WE MOVE FORWARD +C ONE MOTIF (IE IT INCLUDES THE FIRST MOTIF SO IT IS NOT A +C SPECIAL CASE). OTHERWISE WE UPDATE POSITIONS WHEN WE FIND +C A MATCH FOR THEM (WE SET TO THE MATCH POSITION PLUS 1) +C MATCHP = LIST OF CURRENT MATCH POSITIONS FOR EACH MOTIF +C MATCHS = LIST OF CURRENT MATCH SCORES FOR EACH MOTIF +C IFOUND = A FLAG TO INDICATE SUCCESS OR FAILURE OF A SEARCH ROUTINE +C 1 = SUCCESS, 0 = FAIL +C STRNGS = POINTER TO STRING STARTS IN CHARACTER ARRAY STRING +C TEMPORARY VALUES ARE: +C MOTIF = ACTUAL MOTIF NUMBER +C ICLASS = CLASS +C ILEN = LENGTH OF MOTIF +C CUT = CUTOFF +C WT = START OF WEIGHTS FOR THIS MOTIF +C ISTRST = START OF STRING +C RANGET = START OF 3' RANGE FOR STEM SEARCHES +C RANGEM = END OF 3' RANGE FOR STEM SEARCHES +C IENTRY = FLAG TO SIGNIFY MORE 3' STEM POSITIONS FOR LAST 5' START +C 0 = NONE, ON RETURN FROM MOTIF6 IT CONTAINS THE 3' MATCH +C POSITION +C MATCHQ = MATCH POSITION FOR STEM SEARCH +C COMB = LOGICAL COMBINATION A, O, N + INTEGER WTSTR(NMOT),LENGTH(NMOT),CLASS(NMOT) + INTEGER RANGES(NMOT),RANGEL(NMOT),START(NMOT),IEND(NMOT) + INTEGER RELMOT(NMOT),MATCHP(NMOT),STRNGS(NMOT) + INTEGER RANGET(NMOT),RANGEM(NMOT),IENTRY(NMOT),RELEND(NMOT) + INTEGER START2(NMOT),IEND2(NMOT),MATRIX(IDM,IDM),MATCHQ(NMOT) + INTEGER LAST5(NMOT),LAST3S(NMOT),LAST3E(NMOT),MAT1(IDM,IDM) + REAL WEIGHT(MAXWTS),CUTOFF(NMOT),MATCHS(NMOT) + REAL MINSCR,MAXSCR + CHARACTER SEQ(IDSEQ),STRING(MAXSTR) + CHARACTER COMBIN(NMOT),COMB + CHARACTER*(*) KEYNS(NMOT) +C +C +C INITIALIZE + JMOT = 0 + IRET = 0 +5 CONTINUE + JMOT = JMOT + 1 + IF(JMOT.LE.NMOT)THEN + IF(RELMOT(JMOT).EQ.0)THEN + START(JMOT) = RANGES(1) + IEND(JMOT) = RANGES(1) + RANGEL(1) -1 + GO TO 5 + END IF + END IF + MOTIF = 1 + ICLASS = CLASS(1) + ILEN = LENGTH(1) + CUT = CUTOFF(1) + IWT = WTSTR(1) + ISTRST = STRNGS(1) + IENTRY(1) = 0 + COMB = COMBIN(1) + DO 10 I = 1,NMOT + MATCHP(I) = 0 +10 CONTINUE +C +C +C +C +C +100 CONTINUE +C +C +C THIS A CLASS CLASS MOTIF, PERFORM THE APPROPRIATE SEARCH IF THE START +C POSITION IS >0. (IF IT IS NOT THE CURRENT MOTIF IS A NOT THAT HAS +C ALREADY BEEN SEARCHED FOR +C + IFOUND = 0 + IF(START(MOTIF).GT.0)THEN +C +C + IF(ICLASS.EQ.1)THEN + CALL MOTIF1(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF),IFOUND, + + CUTOFF(MOTIF),0) + ELSE IF(ICLASS.EQ.2)THEN + CALL MOTIF2(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),CUT,MATCHP(MOTIF),MATCHS(MOTIF),IFOUND) + ELSE IF(ICLASS.EQ.3)THEN + CALL MOTIF3(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),CUT,MATCHP(MOTIF),MATCHS(MOTIF),IFOUND,MATRIX,IDM) + ELSE IF(ICLASS.EQ.4)THEN + CALL MOTIF4(SEQ,IDSEQ,ILEN,START(MOTIF), + + IEND(MOTIF),WEIGHT(IWT),CUT,MATCHP(MOTIF),MATCHS(MOTIF), + + IFOUND,IDM) + ELSE IF(ICLASS.EQ.5)THEN + CALL MOTIF8(SEQ,IDSEQ,MATRIX,LENGTH(MOTIF),START(MOTIF), + + IEND(MOTIF),RANGET(MOTIF),RANGEM(MOTIF), + + CUTOFF(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF), + + IENTRY(MOTIF),IFOUND,MATCHQ(MOTIF), + + LAST5(MOTIF),LAST3S(MOTIF),LAST3E(MOTIF),IDM) + ELSE IF(ICLASS.EQ.6)THEN + CALL MOTIF4(SEQ,IDSEQ,ILEN,START(MOTIF), + + IEND(MOTIF),WEIGHT(IWT),CUT,MATCHP(MOTIF),MATCHS(MOTIF), + + IFOUND,IDM) +C CALL MOTIF6(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), +C + IEND(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF),IFOUND, +C + CUTOFF(MOTIF),0) + ELSE + WRITE(KBOUT,*)'UNKNOWN CLASS!!' + END IF +C +C + END IF +C +C +C MATCH FOUND WHEN MATCH WANTED ? +C +C +C + IF(((IFOUND.EQ.0).AND.(COMB.NE.'N')).OR. + + ((IFOUND.GT.0).AND.(COMB.EQ.'N')))THEN +C +C NO SO GO BACK OR SIDEWAYS ONE MOTIF +C +C + CALL BAKSID(CLASS,LENGTH,CUTOFF,STRNGS,NMOT, + + MOTIF,ICLASS,ILEN,CUT,IWT,ISTRST,WTSTR, + + RELMOT,START,IEND,MATCHQ,RANGES,RANGEL,RELEND,IRET,MATCHP, + + COMBIN,COMB) +C +C +C IF CANT GO BACK ANY FURTHER QUIT + IF(IRET.NE.0)RETURN +C +C + ELSE +C +C +C MATCH FOUND. +C +C +C PREPARE FOR NEXT SEARCH THIS MOTIF BY INCREMENTING POINTER +C TO SEARCH RANGE (NOT FOR CLASS 6 WHICH IS HANDLED BY MOTIF6) +C +C + IF(COMB.EQ.'N')THEN + START(MOTIF) = -9 + ELSE + IF(ICLASS.NE.5) START(MOTIF) = MATCHP(MOTIF) + 1 + END IF +C +C TRY GOING FORWARD ONE MOTIF +C +C + CALL FORWAD(CLASS,LENGTH,CUTOFF,NMOT, + + MOTIF,ICLASS,ILEN,CUT,IWT,RELMOT,START,IEND, + + RANGES,RANGEL,STRNGS,ISTRST,WTSTR,IDSEQ,IENTRY, + + RANGET,RANGEM,START2,IEND2,MATCHQ,RELEND,MATCHP,IDSPLY, + + COMBIN,COMB) +C +C +C +C IS THIS THE LAST MOTIF? IF SO DISPLAY THE MATCH +C +C + IF(IDSPLY.EQ.1)THEN + ITOTAL = ITOTAL + 1 + CALL DSPLAY(MATCHP,LENGTH,NMOT,SEQ,IDSEQ,IDEVOT, + + CLASS,MATCHQ,MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IOPT, + + XMAX,XMIN,YMAX,YMIN,KSTART,MATCHS,CUTSCR,MINSCR,MAXSCR, + + MATRIX,MAT1,IDM,PMINT,PMAXT,PROBT, + + WEIGHT,MAXWTS,WTSTR,CUTOFF,PMINC,KEYNS) +C +C +C HORRIBLE SPECIAL CASE - IF LAST MOTIF IS NOTTED WE MUST MOVE BACK AFTER +C DISPLAY + IF(COMB.EQ.'N')THEN + CALL BAKSID(CLASS,LENGTH,CUTOFF,STRNGS,NMOT, + + MOTIF,ICLASS,ILEN,CUT,IWT,ISTRST,WTSTR, + + RELMOT,START,IEND,MATCHQ,RANGES,RANGEL,RELEND,IRET,MATCHP, + + COMBIN,COMB) +C +C +C IF CANT GO BACK ANY FURTHER QUIT + IF(IRET.NE.0)RETURN + END IF + END IF +C +C + END IF +C +C +C GO BACK FOR NEXT SEARCH +C +C + GO TO 100 + END + SUBROUTINE GETMK(KBIN,KBOUT,STRING,MAXSTR,ISTRNG, + +LENGTH,MAXMOT,CLASS,RELMOT,RANGES,RANGEL,RANGET,RANGEM, + +STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF,IDEV,WTSTR,JDEV,IOK, + +RELEND,IDSEQ,IDEVSV,IDM,COMBIN,MAXCLS,MATRIX,MAT1, + +PMINT,PMAXT,PROBT,EXPTT,CHRSET, + +IHELPS,IHELPE,HELPF,IDEVH,KEYNS,NAMSAV,FILNAM,TITLE) +C MAT1 SIMPLE IDENTITY +C MAT2 IUB SCORES 0-1 +C MAT3 IUB SCORES 0-36 +C MAT4 INVERTED REPEAT + INTEGER LENGTH(MAXMOT),CLASS(MAXMOT),RELMOT(MAXMOT) + INTEGER RANGES(MAXMOT),RANGEL(MAXMOT),STRNGS(MAXMOT) + INTEGER WTSTR(MAXMOT),RANGET(MAXMOT),RANGEM(MAXMOT) + INTEGER RELEND(MAXMOT),MATRIX(IDM,IDM),MAT1(IDM,IDM) + CHARACTER STRING(MAXSTR),FILNAM*(*),HELPF*(*) + REAL WEIGHT(MAXWTS),CUTOFF(MAXMOT) + CHARACTER COMBIN(MAXMOT),TCLASS,CHRSET(IDM) + CHARACTER*(*) KEYNS(MAXMOT) + CHARACTER*(*) NAMSAV(MAXMOT),TITLE + PARAMETER (MAXPRM = 31) + CHARACTER PROMPT(7)*(MAXPRM) + EXTERNAL PSCORE +C GETS PATTERN DEFINITIONS IN TERMS OF MOTIFS + PMINT = 1.0 + PMAXT = 1.0 + PROBT = 1.0 + EXPTS = 0.0 + PROBS = 0.0 + PMINS = 0.0 + PMAXS = 0.0 + EXPTT = 1.0 + IOK = 0 + IPROB = 0 + DO 10 I=1,MAXMOT + RELEND(I) = 5 + COMBIN(I) = 'A' +10 CONTINUE + RANGES(1) = 0 + RANGEL(1) = IDSEQ + IREL = 0 +C COUNT MOTIFS + NMOT = 0 + MOTIF = 0 +C SET POINTER TO SEARCH STRINGS ARRAY + ISTRNG = 1 +C SET POINTER TO WEIGHT ARRAY + IWT = 1 + TCLASS = 'A' +C SET CLASS TO EXACT MATCH + ICLASS = 1 +100 CONTINUE + IOK = 1 + PROMPT(1) = 'Exact match' + PROMPT(2) = 'Percentage match' + PROMPT(3) = 'Cut-off score and score matrix' + PROMPT(4) = 'Cut-off score and weight matrix' + PROMPT(5) = 'Direct repeat' + PROMPT(6) = 'Membership of set' + PROMPT(7) = 'Pattern complete' + CALL RADION('Select motif definition mode',PROMPT,7,ICLASS, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ICLASS.LT.1) RETURN + IF(ICLASS.EQ.7)GO TO 900 +101 CONTINUE + LKEY = 0 + CALL GTSTR('Motif name',' ',KEYNS(MOTIF+1),LKEY, + +KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 101 + END IF + IF(MOTIF.GT.0) THEN + IF(COMBIN(MOTIF).NE.'N') THEN + IC = 1 + PROMPT(1) = 'And' + PROMPT(2) = 'Or' + PROMPT(3) = 'Not' + CALL RADION('Select logical operator',PROMPT,3,IC, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IC.LT.1) RETURN + IF(IC.EQ.1) TCLASS = 'A' + IF(IC.EQ.2) TCLASS = 'O' + IF(IC.EQ.3) TCLASS = 'N' + ELSE + IC = 1 + PROMPT(1) = 'And' + PROMPT(2) = 'Not' + CALL RADION('Select logical operator',PROMPT,2,IC, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IC.LT.1) RETURN + IF(IC.EQ.1) TCLASS = 'A' + IF(IC.EQ.2) TCLASS = 'N' + END IF + END IF + NMOT = NMOT + 1 + MOTIF = MOTIF + 1 + IF(NMOT.GT.MAXMOT)THEN + WRITE(KBOUT,*)'Maximum number of motifs exceeded' + GO TO 901 + END IF + CLASS(MOTIF) = ICLASS + COMBIN(MOTIF) = TCLASS +C IF NOT THE FIRST MOTIF, AND AN ANDED MOTIF OR THE FIRST IN A LIST OF ORS +C GET ITS RANGE ETC + IOK = 0 + IF((MOTIF.GT.1).AND.(COMBIN(MOTIF).NE.'O'))THEN +C WRITE(KBOUT,1002) +C1002 FORMAT(' RANGES ARE DEFINED RELATIVE TO OTHER MOTIFS',/, +C +' AND BY STARTS AND DISTANCES.') + IOK = 1 + MININ = 1 + MAXIN = MOTIF +211 CONTINUE + MAXIN = MAXIN - 1 + IF(COMBIN(MAXIN).NE.'A') GO TO 211 + IREL = MAXIN + CALL GETINT(MININ,MAXIN,IREL,'Number of reference motif', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IREL = IVAL + RELMOT(MOTIF) = IREL + IOK = 1 + RELEND(MOTIF) = 5 + MININ = -1000 + MAXIN = 1000 + ID = LENGTH(IREL) + 1 + IF(CLASS(IREL).EQ.5) ID = 1 + CALL GETINT(MININ,MAXIN,ID,'Relative start position', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + ID = IVAL + RANGES(MOTIF) = ID - 1 + MININ = 0 + MAXIN = 1000 + IR = 0 + CALL GETINT(MININ,MAXIN,IR,'Number of extra positions', + + IVAL,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IR = IVAL + RANGEL(MOTIF) = IR + ELSE IF(COMBIN(MOTIF).EQ.'O')THEN +C NEED TO SET RANGES TO THOSE OF THE FIRST IN A SET OF ORED MOTIFS +C SET TO THOSE OF THE PREVIOUS MOTIF BECAUSE IT MUST BE THE SAME + RANGES(MOTIF) = RANGES(MOTIF-1) + RANGEL(MOTIF) = RANGEL(MOTIF-1) + RELEND(MOTIF) = RELEND(MOTIF-1) + RELMOT(MOTIF) = IREL + END IF + CALL GETMC(KBIN,KBOUT,STRING,MAXSTR,ISTRNG, + +LENGTH,MAXMOT,CLASS,RELMOT,RANGES,RANGEL,RANGET,RANGEM, + +STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF,IDEV,WTSTR,JDEV,IOK, + +RELEND,IDSEQ,IDEVSV,IDM,COMBIN,MAXCLS,MATRIX,MAT1, + +PMINT,PMAXT,PROBT,EXPTT,EXPTS,PROBS,PMINS,PMAXS,CHRSET, + +IHELPS,IHELPE,HELPF,IDEVH,MOTIF,IWT,NAMSAV,FILNAM,IPROB) + IF(IOK.EQ.0) GO TO 100 +901 CONTINUE + WRITE(KBOUT,*)' Error in pattern definition' + IOK = 1 + RETURN +900 CONTINUE + EXPTT = EXPTT * EXPTS + PROBT = PROBT * PROBS + PMINT = PMINT * PMINS + PMAXT = PMAXT * PMAXS + CALL YESNO(IN,'Save pattern in a file', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IN.LT.0)RETURN + IF(IN.EQ.0)THEN + FILNAM = ' ' + CALL OPENF1(IDEVSV,FILNAM,1,IOK,KBIN,KBOUT, + + 'Pattern definition file', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + CALL SAVSIG( + + IDEVSV,STRING,MAXSTR, + + LENGTH,CLASS,RELMOT,RANGES,RANGEL, + + RANGET,RANGEM, + + STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF, + + WTSTR,RELEND,NAMSAV,COMBIN,KEYNS,IDEV,FILNAM, + + KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,CHRSET,IDM,TITLE) + CLOSE(UNIT=IDEVSV) + END IF + DO 899 I = 2,NMOT + IF(CLASS(I).EQ.5) THEN + RANGEL(I) = RANGEL(I) + 1 + ELSE + RANGEL(I) = RANGEL(I) + LENGTH(I) + END IF +899 CONTINUE +C RETURN STRING LENGTH + MAXSTR = ISTRNG - 1 + IOK = 0 + END + SUBROUTINE DSPLAY(MATCHP,LENGTH,NMOT,SEQ,IDSEQ,IDEV, + +CLASS,MATCHQ,MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX,IOPT, + +XMAX,XMIN,YMAX,YMIN,KSTART,MATCHS,CUTSCR,MINSCR,MAXSCR, + +MATRIX,MAT1,IDM,PMINT,PMAXT,PROBT, + +WEIGHT,MAXWTS,WTSTR,CUTOFF,PMINC,KEYNS) + INTEGER MATCHP(NMOT),LENGTH(NMOT),CLASS(NMOT) + INTEGER MATCHQ(NMOT) + CHARACTER SEQ(IDSEQ) + REAL MATCHS(NMOT),MINSCR,MAXSCR + REAL WEIGHT(MAXWTS),CUTOFF(NMOT) + INTEGER MAT1(IDM,IDM),MATRIX(IDM,IDM) + INTEGER WTSTR(NMOT) + CHARACTER*(*) KEYNS(NMOT) + EXTERNAL PSCORE +C ADD SCORES + T = 0. + DO 10 I = 1,NMOT + IF(MATCHP(I).NE.0) T = T + MATCHS(I) +10 CONTINUE + POBS = 1.0 + IF((PMINC.LT.1.0).OR.(IOPT.EQ.3))THEN +C CALC PROB + DO 20 I = 1,NMOT + IF(MATCHP(I).NE.0)THEN + CALL GETP(CLASS(I),SEQ(MATCHP(I)+KSTART-1),LENGTH(I), + + IDM,MATRIX,MAT1,WEIGHT(MAX(1,WTSTR(I)))) + PROB = PSCORE(MATCHS(I)) + POBS = POBS * PROB + END IF +20 CONTINUE + IF((PMINC.LT.1.0).AND.(POBS.GT.PMINC))RETURN + END IF +C + IF(T.GT.MAXSCR) MAXSCR = T + IF(T.LT.MINSCR) MINSCR = T + IF(T.LT.CUTSCR) RETURN +C + IF(IOPT.EQ.1)THEN + WRITE(IDEV,1001) +1001 FORMAT(' Match') + DO 100 I=1,NMOT + J = I +C CHECK FOR NO MATCH (NEEDED FOR ORED MOTIFS) + IF(MATCHP(J).NE.0)THEN + WRITE(IDEV,1000)MATCHP(J)+KSTART-1,KEYNS(I) + WRITE(IDEV,1002)(SEQ(K),K=MATCHP(J),MATCHP(J)+LENGTH(J)-1) +C REPEAT ? + IF(CLASS(J).EQ.5)THEN + WRITE(IDEV,1002)(SEQ(K),K=MATCHQ(J),MATCHQ(J)+LENGTH(J)-1) + WRITE(IDEV,1000)MATCHQ(J)+KSTART-1 + END IF + END IF +100 CONTINUE +1000 FORMAT(' ',I7,' ',A8) +1002 FORMAT(' ',60A1) + IF(PMINC.LT.1.0)WRITE(IDEV,1004)POBS +1004 FORMAT(' Probability =',E10.4) + RETURN + END IF + IF(IOPT.EQ.3)THEN +C MARK THE POSITION OF THE FIRST NON-ZERO MATCH ONLY + DO 200 I = 1,NMOT + IF(MATCHP(I).NE.0)THEN + X = MATCHP(I) + KSTART - 1 + Y = LOG(1.-POBS) + CALL VECTOM + CALL LINE(X,X,YMIN,Y,XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB,MARGT,ISXMAX,ISYMAX) + CALL VT100M + GO TO 201 + END IF +200 CONTINUE +201 CONTINUE + END IF + IF(IOPT.EQ.2)THEN + MINP = 999999 + MAXP = -999999 + DO 300 I = 1, NMOT + K = MATCHP(I) + IF(K.NE.0)THEN + IF(K.LT.MINP)MINP = K + K = K + LENGTH(I) - 1 +C REPEAT ? + IF(CLASS(I).EQ.5) K = MATCHQ(I) + LENGTH(I) - 1 + IF(K.GT.MAXP)MAXP = K + END IF +300 CONTINUE + WRITE(IDEV,1000)MINP+KSTART-1 + WRITE(IDEV,1002) + + ((SEQ(K1),K1=K2,MIN(K2+59,MAXP)),K2=MINP,MAXP,60) + IF(PMINC.LT.1.0)WRITE(IDEV,1004)POBS + RETURN + END IF + IF(IOPT.EQ.4)THEN + MINP = 999999 + MAXP = -999999 + K1 = 0 + DO 400 I = 1, NMOT + K = MATCHP(I) + IF(K.NE.0)THEN + IF(K1.EQ.0) INAM = I + K1 = 1 + IF(K.LT.MINP)MINP = K + K = K + LENGTH(I) - 1 +C REPEAT ? + IF(CLASS(I).EQ.5) K = MATCHQ(I) + LENGTH(I) - 1 + IF(K.GT.MAXP)MAXP = K + END IF +400 CONTINUE + CALL EMBOUT(KEYNS(INAM),MINP+KSTART-1,MAXP+KSTART-1,' ', + + 'Program',IDEV) + END IF + END + SUBROUTINE MOTIF3(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND,CUTOFF, + +MATCHP,MATCHS,IFOUND,MATRIX,IDM) + CHARACTER SEQ(IDIM1),STRING(IDIM2) + INTEGER MATRIX(IDM,IDM) + REAL MATCHS + IFOUND = 0 + IF(ISTART.LT.1)ISTART=1 + IF(ISTART.GT.IDIM1)RETURN + CALL SQFIT5(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND,CUTOFF,MATCHS, + +IFOUND,MATRIX,IDM) + IF(IFOUND.EQ.0)RETURN +C SAVE MATCH POSITION + MATCHP = IFOUND + RETURN + END +C********************************************************************* + SUBROUTINE SQFIT5(SEQ,IDIM1,STRING,IDIM2, + 1IS,IE,MINSC,MATCHS,IFOUND,MATRIX,IDM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2) + REAL MATCHS,MINSC + INTEGER MATRIX(IDM,IDM),DTONUM + EXTERNAL DTONUM + MINSCR = MINSC +C + IDIF=(IE-IS+2)-IDIM2 +C IDIF IS THE NUMBER OF POSNS TO TRY +C IPSTR GOES FROM 1 TO IDIM2 IDIF TIMES +C TRY ALL POSSIBLE POSITIONS FOR MATCHING AND SCORE FOR EACH +C POINT TO ARRAY ELEMENT CORRESPONDING TO FIRST BASE + IPSEQ=IS + DO 200 I=1,IDIF + NTOT=0 + IP=IPSEQ + DO 100 J=1,IDIM2 + NTOT = NTOT + MATRIX(DTONUM(SEQ(IP)),DTONUM(STRING(J))) + IP=IP+1 +100 CONTINUE +C END OF COUNTING FOR THIS POSITION.IS TOTAL HIGH ENOUGH? + IF(NTOT.GE.MINSCR)THEN + MATCHS = NTOT + IFOUND = IP-IDIM2 + RETURN + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + IFOUND = 0 + RETURN + END +C********************************************************************* + SUBROUTINE MOTIF4(SEQ,IDIM1,LENGTH,ISTART,IEND, + +WEIGHT,CUTOFF,MATCHP,MATCHS,IFOUND,IDM) + REAL WEIGHT(IDM,LENGTH) + REAL MATCHS + CHARACTER SEQ(IDIM1) + IFOUND = 0 + IF(ISTART.LT.1)ISTART=1 + L1 = IEND-ISTART+1 + IF(ISTART.GT.IDIM1)RETURN + IF(L1.LT.LENGTH)RETURN + CALL FMOT4(SEQ(ISTART),L1,WEIGHT,LENGTH,CUTOFF,SCORE,IFOUND,IDM) + IF(IFOUND.EQ.0)RETURN +C SAVE MATCH POSITION + MATCHP = ISTART+IFOUND-1 + MATCHS = SCORE + END +C********************************************************************* + SUBROUTINE FMOT4(SEQ,IDIM,WT,LENGTH,CUTOFF,SUM,IFOUND,IDM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + REAL WT(IDM,LENGTH) + INTEGER CTONUM + EXTERNAL CTONUM + DO 10 I=1,IDIM-LENGTH+1 + SUM=0. + K=0 + DO 5 J=I,I+LENGTH-1 + K=K+1 + IP = CTONUM(SEQ(J)) + SUM=SUM+WT(IP,K) +5 CONTINUE + IF(SUM.GE.CUTOFF)THEN + IFOUND = I + RETURN + END IF +10 CONTINUE + IFOUND = 0 + END +C********************************************************************* + SUBROUTINE MOTIF8(SEQ,IDSEQ,MATRIX,LENGTH,I5STAR,I5END, + + I3STAR,I3END,CUTOFF,MATCHP,MATCHS, + + IENTRY,IFOUND,MATCHQ, + + LAST5,LAST3S,LAST3E,IDM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ) + INTEGER MATRIX(IDM,IDM),REPEAT + REAL MATCHS + EXTERNAL REPEAT +C WE HAVE A START POSITION FOR THE 5' END OF THE 5' END OF +C A POTENTIAL REPEAT I5STAR AND AN END DEFINED BY A RANGE I5END +C WE HAVE A REPEAT LENGTH LENGTH +C WE HAVE A RANGE OF POSITIONS FOR THE 3' STEM TO START +C I3STAR TO I3END +C TRY THE TIGHTEST LOOPS FIRST +C BUT FIRST WE MAY HAVE TO FINISH A PREVIOUS SEARCH +C THIS IS DENOTED BY IENTRY NE 0. +C NOTE IENTRY IS ALSO USED TO RETURN THE 3' MATCH POSITION + ICUT = CUTOFF +C WRITE(*,*)'ICUT',ICUT + IFOUND = 0 + JENTRY = IENTRY + IENTRY = 0 + IF(I5STAR.LT.1)I5STAR=1 + IF((I5STAR+I3STAR+LENGTH-2).GT.IDSEQ)RETURN + IF(JENTRY.NE.0)THEN + I1 = LAST5 +C WRITE(*,*)'I1,LAST3S,LAST3E',I1,LAST3S,LAST3E + DO 50 J=LAST3S+1,LAST3E + J1 = J + ISUM = REPEAT(SEQ,IDSEQ,MATRIX,LENGTH,I1,J1,IDM) +C RETURN IF GOOD ENOUGH + IF(ISUM.GE.ICUT)THEN + MATCHP = I1 + IENTRY = J1 + MATCHQ = J1 + MATCHS = ISUM + IFOUND = MATCHP + LAST3S = J1 + RETURN + END IF +50 CONTINUE +C NOW MOVE 5' STEM START POSITION (WE HAVE JUST FINISHED THE LAST) +C TO THE LAST MATCH + 1 + I5STAR = MATCHP + 1 + END IF +C SET ENTRY FLAG TO ZERO TO SIGNIFY LAST SEARCH NOW COMPLETED + IENTRY = 0 + ISUM = 0 + LOOPI1 = I5STAR + IF((I5STAR+I3STAR+LENGTH-2).GT.IDSEQ)RETURN + LOOPI2 = MIN(IDSEQ-2*LENGTH+1,I5END) +C WRITE(*,*)'IDSEQ,LENGTH,I5STAR,I5END', +C +IDSEQ,LENGTH,I5STAR,I5END +C +C TRY ALL STEM STARTS FROM 5' START TO 5' END +C +C + DO 200 I = LOOPI1,LOOPI2 +C +C + I1 = I +C +C TRY ALL LOOPS FROM 3' START TO 3' END +C + LOOPJ1 = I + I3STAR -1 + IF((LOOPJ1+LENGTH-1).GT.IDSEQ)RETURN + LOOPJ2 = MIN(IDSEQ-LENGTH+1,I+I3END-1) +C WRITE(*,*)'I3STAR,I3END',I3STAR,I3END +C +C +C + DO 100 J = LOOPJ1,LOOPJ2 +C +C +C + J1 = J +C IN REPEAT NOTE THAT +C THE 5' END POINTER I1 GOES FORWARDS +C THE 3' END POINTER J1 GOES FORWARDS +C + ISUM = REPEAT(SEQ,IDSEQ,MATRIX,LENGTH,I1,J1,IDM) +C RETURN IF GOOD ENOUGH +C WRITE(*,*)ISUM + IF(ISUM.GE.ICUT)THEN + MATCHP = I1 + IENTRY = J1 + MATCHQ = J1 + MATCHS = ISUM + IFOUND = MATCHP +C SAVE CURRENT POSITION FOR LATER ENTRIES + LAST5 = I1 + LAST3S = J1 + LAST3E = LOOPJ2 + RETURN + END IF +100 CONTINUE +200 CONTINUE + END +C********************************************************************* + INTEGER FUNCTION REPEAT(SEQ,IDSEQ,MATRIX,LENGTH,I5P,I3P,IDM) + CHARACTER SEQ(IDSEQ) + INTEGER MATRIX(IDM,IDM),CTONUM + EXTERNAL CTONUM +C THE 5' END POINTER GOES FORWARDS +C THE 3' END POINTER GOES FORWARDS + L=0 + I5=I5P-1 + I3=I3P-1 + DO 100 I=1,LENGTH + I5 = I5 + 1 + I3 = I3 + 1 +C WRITE(*,*)'I5,I3',I5,I3 + L5 = CTONUM(SEQ(I5)) + L3 = CTONUM(SEQ(I3)) + L = L + MATRIX(L5,L3) +100 CONTINUE + REPEAT = L + END + SUBROUTINE MOTIF6(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND, + +MATCHP,MATCHS,IFOUND,CUT,JOB) + CHARACTER SEQ(IDIM1),STRING(IDIM2) + REAL MATCHS + INTEGER CUT + INTEGER INSET1 + EXTERNAL INSET1 + IFOUND = 0 + ISTEP = 1 +C IF(JOB.EQ.1)ISTEP = CUT + IF(ISTART.LT.1)ISTART=1 + L1 = IEND-ISTART+1 + IF(ISTART.GT.IDIM1)RETURN + IF(L1.LT.1)RETURN + IFOUND = INSET1(SEQ(ISTART),L1,STRING,IDIM2,ISTEP) + IF(IFOUND.EQ.0)RETURN +C SAVE MATCH POSITION + MATCHP = ISTART+IFOUND-1 + MATCHS = 1. + END + INTEGER FUNCTION INSET1(SEQ,IDIM,STRING,IDS,INC) + CHARACTER SEQ(IDIM),STRING(IDS) + INTEGER INSET + EXTERNAL INSET + INSET1 = 0 + ISEQ = 1 - INC +10 CONTINUE + ISEQ = ISEQ + INC + IF(ISEQ.GT.IDIM)RETURN + IMATCH = INSET(SEQ(ISEQ),STRING,IDS) + IF(IMATCH.EQ.1)THEN + INSET1 = ISEQ + RETURN + END IF + GO TO 10 + END + INTEGER FUNCTION INSET(SEQ,STRING,IDS) + CHARACTER SEQ,STRING(IDS) + INTEGER CTONUM + EXTERNAL CTONUM + INSET = 1 + KSEQ = CTONUM(SEQ) + DO 20 I = 1,IDS + IF(KSEQ.EQ.CTONUM(STRING(I)))RETURN +20 CONTINUE + INSET = 0 + END + SUBROUTINE SETCMP(SEQ,IDIM,IDM) + PARAMETER (MAXCHR = 26) + CHARACTER SEQ(IDIM) + INTEGER DTONUM + EXTERNAL DTONUM + COMMON /COMPC/COMP(MAXCHR) + SAVE /COMPC/ + DO 10 I = 1,IDM + COMP(I) = 0.0 +10 CONTINUE + DO 20 I = 1,IDIM + J = DTONUM(SEQ(I)) + COMP(J) = COMP(J) + 1. +20 CONTINUE + T = MAX(1,IDIM) + DO 30 I = 1,IDM + COMP(I) = COMP(I) / T +30 CONTINUE + END diff --git a/src/staden/patternpc.f b/src/staden/patternpc.f new file mode 100644 index 0000000..56e11d4 --- /dev/null +++ b/src/staden/patternpc.f @@ -0,0 +1,1593 @@ +C 9-11-90 Large number of changes relating to use of file of +C file names and removal of radio +C 3-7-91 Removed annotation "filename" from pattern files +C 18-7-91 Added titles to pattern files +C 2-3-92 set filnam = ' ' for calls to openf1 + SUBROUTINE GETMF(KBIN,KBOUT,STRING,MAXSTR,ISTRNG, + +LENGTH,MAXMOT,CLASS,RELMOT,RANGES,RANGEL,RANGET,RANGEM, + +STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF,IDEV,WTSTR,JDEV,IOK, + +RELEND,IDSEQ,IDEVSV,IDM,COMBIN,MAXCLS,MATRIX,MAT1, + +PMINT,PMAXT,PROBT,EXPTT,CHRSET, + +IHELPS,IHELPE,HELPF,IDEVH,KEYNS,NAMSAV,FILNAM,IPROB,TITLE) + INTEGER LENGTH(MAXMOT),CLASS(MAXMOT),RELMOT(MAXMOT) + INTEGER RANGES(MAXMOT),RANGEL(MAXMOT),STRNGS(MAXMOT) + INTEGER WTSTR(MAXMOT),RANGET(MAXMOT),RANGEM(MAXMOT) + INTEGER RELEND(MAXMOT),MATRIX(IDM,IDM),MAT1(IDM,IDM) + CHARACTER STRING(MAXSTR),FILNAM*(*),HELPF*(*) + REAL WEIGHT(MAXWTS),CUTOFF(MAXMOT) + CHARACTER COMBIN(MAXMOT),TCLASS,CHRSET(IDM) + CHARACTER*(*) NAMSAV(MAXMOT),KEYNS(MAXMOT),TITLE + EXTERNAL PSCORE +C GETS PATTERN DEFINITIONS IN TERMS OF MOTIFS + PMINT = 1.0 + PMAXT = 1.0 + PROBT = 1.0 + EXPTS = 0.0 + PROBS = 0.0 + PMINS = 0.0 + PMAXS = 0.0 + EXPTT = 1.0 + IOK = 1 + DO 10 I=1,MAXMOT + RELEND(I) = 5 + COMBIN(I) = 'A' +10 CONTINUE + RANGES(1) = 0 + RANGEL(1) = IDSEQ + IREL = 0 +C COUNT MOTIFS + NMOT = 0 + MOTIF = 0 +C SET POINTER TO SEARCH STRINGS ARRAY + ISTRNG = 1 +C SET POINTER TO WEIGHT ARRAY + IWT = 1 +C get title + READ(JDEV,1000,ERR=901,END=901)TITLE + 1000 FORMAT(A) +100 CONTINUE + CALL GETCLS(JDEV,KBOUT,ICLASS,TCLASS,KEYNS(MOTIF+1)) +1001 FORMAT(I7) + IF(ICLASS.LT.0) GO TO 901 + IF(ICLASS.GT.MAXCLS)GO TO 901 + IF(ICLASS.EQ.0)GO TO 900 + IF((TCLASS.EQ.'O').AND.(NMOT.LT.1))THEN + WRITE(KBOUT,*)'CANNOT OR FIRST MOTIF' + GO TO 901 + END IF + IF((TCLASS.EQ.'N').AND.(NMOT.LT.1))THEN + WRITE(KBOUT,*)'CANNOT NOT FIRST MOTIF' + GO TO 901 + END IF + IF((TCLASS.EQ.'O').AND.(COMBIN(MOTIF).EQ.'N'))THEN + WRITE(KBOUT,*)'CANNOT OR WITH NOTTED MOTIF' + GO TO 901 + END IF + NMOT = NMOT + 1 + MOTIF = MOTIF + 1 + IF(NMOT.GT.MAXMOT)THEN + WRITE(KBOUT,*)'MAXIMUM NUMBER OF MOTIFS EXCEEDED' + GO TO 901 + END IF + CLASS(MOTIF) = ICLASS + COMBIN(MOTIF) = TCLASS +C IF NOT THE FIRST MOTIF, AND AN ANDED MOTIF OR THE FIRST IN A LIST OF ORS +C GET ITS RANGE ETC + IF((MOTIF.GT.1).AND.(COMBIN(MOTIF).NE.'O'))THEN + READ(JDEV,1001,ERR=901)IREL + IF(IREL.LT.0)GO TO 901 + IF(IREL.LT.1)GO TO 901 + IF(IREL.GT.MOTIF-1)THEN + WRITE(KBOUT,*)'CAN ONLY REFER BACK' + GO TO 901 + END IF + IF(COMBIN(IREL).NE.'A')THEN + WRITE(KBOUT,*)'CAN ONLY REFER TO AN ANDED MOTIF' + GO TO 901 + END IF + RELMOT(MOTIF) = IREL + READ(JDEV,1001,ERR=901)ID + READ(JDEV,1001,ERR=901)IR + IF(IR.LT.0)GO TO 901 + RANGES(MOTIF) = ID - 1 + RANGEL(MOTIF) = IR + ELSE IF(COMBIN(MOTIF).EQ.'O')THEN +C NEED TO SET RANGES TO THOSE OF THE FIRST IN A SET OF ORED MOTIFS +C SET TO THOSE OF THE PREVIOUS MOTIF BECAUSE IT MUST BE THE SAME + RANGES(MOTIF) = RANGES(MOTIF-1) + RANGEL(MOTIF) = RANGEL(MOTIF-1) + RELEND(MOTIF) = RELEND(MOTIF-1) + RELMOT(MOTIF) = IREL + END IF + XRAN = 1.0 +C NOW GET DETAILS SPECIFIC TO EACH CLASS OF MOTIF + CALL GETMC(KBIN,KBOUT,STRING,MAXSTR,ISTRNG, + +LENGTH,MAXMOT,CLASS,RELMOT,RANGES,RANGEL,RANGET,RANGEM, + +STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF,IDEV,WTSTR,JDEV,IOK, + +RELEND,IDSEQ,IDEVSV,IDM,COMBIN,MAXCLS,MATRIX,MAT1, + +PMINT,PMAXT,PROBT,EXPTT,EXPTS,PROBS,PMINS,PMAXS,CHRSET, + +IHELPS,IHELPE,HELPF,IDEVH,MOTIF,IWT,NAMSAV,FILNAM,IPROB) + IF(IOK.EQ.0) GO TO 100 +901 CONTINUE + WRITE(KBOUT,*)' Error in pattern definition' + IOK = 1 + CLOSE(UNIT=JDEV) + RETURN +900 CONTINUE + EXPTT = EXPTT * EXPTS + PROBT = PROBT * PROBS + PMINT = PMINT * PMINS + PMAXT = PMAXT * PMAXS + CLOSE(UNIT=JDEV) + DO 899 I = 2,NMOT + IF(CLASS(I).EQ.5) THEN + RANGEL(I) = RANGEL(I) + 1 + ELSE + RANGEL(I) = RANGEL(I) + LENGTH(I) + END IF +899 CONTINUE +C RETURN STRING LENGTH + MAXSTR = ISTRNG - 1 + IOK = 0 + END + SUBROUTINE GETMC(KBIN,KBOUT,STRING,MAXSTR,ISTRNG, + +LENGTH,MAXMOT,CLASS,RELMOT,RANGES,RANGEL,RANGET,RANGEM, + +STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF,IDEV,WTSTR,JDEV,IOK, + +RELEND,IDSEQ,IDEVSV,IDM,COMBIN,MAXCLS,MATRIX,MAT1, + +PMINT,PMAXT,PROBT,EXPTT,EXPTS,PROBS,PMINS,PMAXS,CHRSET, + +IHELPS,IHELPE,HELPF,IDEVH,MOTIF,IWT,NAMSAV,FILNAM,IPROB) + INTEGER LENGTH(MAXMOT),CLASS(MAXMOT),RELMOT(MAXMOT) + INTEGER RANGES(MAXMOT),RANGEL(MAXMOT),STRNGS(MAXMOT) + INTEGER WTSTR(MAXMOT),RANGET(MAXMOT),RANGEM(MAXMOT) + INTEGER RELEND(MAXMOT),MATRIX(IDM,IDM),MAT1(IDM,IDM) + CHARACTER STRING(MAXSTR),FILNAM*(*),HELPF*(*) + REAL WEIGHT(MAXWTS),CUTOFF(MAXMOT) + CHARACTER COMBIN(MAXMOT),CHRSET(IDM) + CHARACTER*(*) NAMSAV(MAXMOT) + EXTERNAL PSCORE + XRAN = 1.0 + PMIN = 1. + PMAX = 1. + PROB = 1. +C NOW GET DETAILS SPECIFIC TO EACH CLASS OF MOTIF + IF(CLASS(MOTIF).EQ.1)THEN + MXSTR = MAXSTR - ISTRNG + 1 + CALL GETMT1(STRING(ISTRNG),MXSTR,LENGTH(MOTIF),KBIN,KBOUT, + + IOK,JDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM,MATRIX,MAT1,WEIGHT(IWT)) + SCMIN = LENGTH(MOTIF) + PROB = PSCORE(SCMIN) + PMIN = PROB + PMAX = PROB + END IF + STRNGS(MOTIF) = ISTRNG + ISTRNG = ISTRNG + LENGTH(MOTIF) + CUTOFF(MOTIF) = LENGTH(MOTIF) + ELSE IF(CLASS(MOTIF).EQ.2)THEN + MXSTR = MAXSTR - ISTRNG + 1 + CALL GETMT2(STRING(ISTRNG),MXSTR,LENGTH(MOTIF), + + CUTOFF(MOTIF),KBIN,KBOUT,IOK,JDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM,MATRIX,MAT1,WEIGHT(IWT)) + SCMIN = CUTOFF(MOTIF) + SCMAX = LENGTH(MOTIF) + PROB = PSCORE(SCMIN) + PMIN = PROB + PMAX = PSCORE(SCMAX) + END IF + STRNGS(MOTIF) = ISTRNG + ISTRNG = ISTRNG + LENGTH(MOTIF) + ELSE IF(CLASS(MOTIF).EQ.3)THEN + MXSTR = MAXSTR - ISTRNG + 1 + CALL GETMT3(STRING(ISTRNG),MXSTR,LENGTH(MOTIF), + + CUTOFF(MOTIF),MATRIX,IDM,KBIN,KBOUT,IOK,JDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM,MATRIX,MAT1,WEIGHT(IWT)) + SCMIN = CUTOFF(MOTIF) + SCMAX = LENGTH(MOTIF) * 15.7 + PROB = PSCORE(SCMIN) + PMAX = PSCORE(SCMAX) + PMIN = PROB + END IF + STRNGS(MOTIF) = ISTRNG + ISTRNG = ISTRNG + LENGTH(MOTIF) + ELSE IF(CLASS(MOTIF).EQ.4)THEN + MXWT = MAXWTS - IWT + 1 + CALL GETMT4(WEIGHT(IWT),MXWT,LENGTH(MOTIF),CUTOFF(MOTIF), + + SCMAX,IDEV,KBIN,KBOUT,IOK,JDEV,FILNAM,IDM, + + IHELPS,IHELPE,HELPF,IDEVH,IPROB) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM,MATRIX,MAT1,WEIGHT(IWT)) + SCMIN = CUTOFF(MOTIF) + PROB = PSCORE(SCMIN) + PMAX = PSCORE(SCMAX) + PMIN = PROB + END IF + WTSTR(MOTIF) = IWT + IWT = IWT + LENGTH(MOTIF)*IDM + NAMSAV(MOTIF) = FILNAM + ELSE IF(CLASS(MOTIF).EQ.5)THEN + CALL GETMT8(RANGET(MOTIF),RANGEM(MOTIF),LENGTH(MOTIF), + + CUTOFF(MOTIF),KBIN,KBOUT,JDEV,IOK, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM,MATRIX,MAT1,WEIGHT(IWT)) + SCMIN = CUTOFF(MOTIF) + SCMAX = LENGTH(MOTIF) * 15.7 + PROB = PSCORE(SCMIN) + PMAX = PSCORE(SCMAX) + PMIN = PROB + END IF + XRAN = ABS(RANGEM(MOTIF))-ABS(RANGET(MOTIF))+1 + ELSE IF(CLASS(MOTIF).EQ.6)THEN + MXSTR = MAXSTR - ISTRNG + 1 + MXWT = MAXWTS - IWT + 1 + FILNAM = ' ' + CALL GETMT6(STRING(ISTRNG),MXSTR,LENGTH(MOTIF),WEIGHT(IWT), + + MXWT,CUTOFF(MOTIF),KBIN,KBOUT,JDEV,IDEV,IDM, + + FILNAM,IOK, + + IHELPS,IHELPE,HELPF,IDEVH,IPROB) + IF(IOK.NE.0)GO TO 901 + IF(IPROB.EQ.0) THEN + CALL GETP(CLASS(MOTIF),STRING(ISTRNG), + + LENGTH(MOTIF),IDM,MATRIX,MAT1,WEIGHT(IWT)) + SCMIN = CUTOFF(MOTIF) + SCMAX = SCMIN + PROB = PSCORE(SCMIN) + PMAX = PSCORE(SCMAX) + PMIN = PROB + END IF + WTSTR(MOTIF) = IWT + IWT = IWT + LENGTH(MOTIF)*IDM + IF(FILNAM.NE.' ') NAMSAV(MOTIF) = FILNAM + END IF +C GET RANGE OF POSITIONS FOR PROBABILITY CALC + LRANGE = RANGEL(MOTIF) + 1 + IF(COMBIN(MOTIF).EQ.'A')THEN + IF(EXPTS.GT.0.0)THEN + EXPTT = EXPTT * EXPTS + PROBT = PROBT * PROBS + PMINT = PMINT * PMINS + PMAXT = PMAXT * PMAXS + END IF + EXPTS = LRANGE * XRAN * PROB + PROBS = PROB + PMINS = PMIN + PMAXS = PMAX + ELSE IF(COMBIN(MOTIF).EQ.'N')THEN + IF(EXPTS.GT.0.0)THEN + EXPTT = EXPTT * EXPTS + PROBT = PROBT * PROBS + PMINT = PMINT * PMINS + PMAXT = PMAXT * PMAXS + END IF + PMIN = 1.0 - PMIN + PMAX = 1.0 - PMAX + PROB = 1.0 - PROB + EXPTS = PROB ** (LRANGE * XRAN) + PROBS = PROB + PMINS = PMIN + PMAXS = PMAX + ELSE IF(COMBIN(MOTIF).EQ.'O')THEN + EXPTS = EXPTS + LRANGE * XRAN * PROB + PROBS = PROBS + PROB + PMINS = PMINS + PMIN + PMAXS = PMAXS + PMAX + END IF + IF(IPROB.EQ.0) CALL WRTSCR(SCMIN,PROB,KBOUT) + IOK = 0 + RETURN +901 CONTINUE + IOK = 1 + END +C********************************************************************* + SUBROUTINE DESSIG( + + KBOUT,STRING,MAXSTR, + + LENGTH,CLASS,RELMOT,RANGES,RANGEL, + + RANGET,RANGEM, + + STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF, + + WTSTR,RELEND,COMBIN,KEYNS,TITLE) + CHARACTER STRING(MAXSTR) + INTEGER LENGTH(NMOT),CLASS(NMOT),RELMOT(NMOT),RELEND(NMOT) + INTEGER RANGES(NMOT),RANGEL(NMOT),RANGET(NMOT),RANGEM(NMOT) + INTEGER WTSTR(NMOT),STRNGS(NMOT) + REAL WEIGHT(MAXWTS),CUTOFF(NMOT) + CHARACTER COMBIN(NMOT) + CHARACTER*(*) KEYNS(NMOT),TITLE +C +C +C DESCRIBE THE SIGNAL +C + WRITE(KBOUT,1000) +1000 FORMAT(/' Pattern description',/) + WRITE(KBOUT,1006)TITLE + 1006 FORMAT(' ',A) + DO 100 I = 1,NMOT + WRITE(KBOUT,1001)I,KEYNS(I),CLASS(I) +1001 FORMAT(' Motif ',I2,' named ',A8,' is of class ',I4) +C + IF(CLASS(I).EQ.1)THEN + WRITE(KBOUT,1002) + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) +1002 FORMAT(' Which is an exact match to the string', + + /,(' ',50A1)) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I)-LENGTH(I)+1,RELMOT(I) +1008 FORMAT(' and the N-terminal residue can take positions', + + I7,' to ',I7,/, + + ' relative to the N-terminal end of motif',I4) + ELSE IF(CLASS(I).EQ.2)THEN + WRITE(KBOUT,1003)CUTOFF(I), + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) +1003 FORMAT(' which is a match of score ',F6.0, + + ' to the string',/,(' ',50A1)) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I)-LENGTH(I)+1,RELMOT(I) + ELSE IF(CLASS(I).EQ.3)THEN + WRITE(KBOUT,1003)CUTOFF(I), + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I)-LENGTH(I)+1,RELMOT(I) + ELSE IF(CLASS(I).EQ.4)THEN + WRITE(KBOUT,1004)CUTOFF(I) +1004 FORMAT(' Which is a match to a weight matrix with score', + + F8.3) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I)-LENGTH(I)+1,RELMOT(I) + ELSE IF(CLASS(I).EQ.5)THEN + WRITE(KBOUT,1014)LENGTH(I),CUTOFF(I) +1014 FORMAT(' Which is a repeat with repeat length',I5, + + ' and score ',F6.0) + WRITE(KBOUT,1015)RANGET(I)-LENGTH(I)-1,RANGEM(I)-LENGTH(I)-1 +1015 FORMAT(' The loop-out can have sizes ',I6,' to ',I6) + IF(I.NE.1) + + WRITE(KBOUT,1008)RANGES(I)+1, + + RANGES(I)+RANGEL(I),RELMOT(I) + ELSE IF(CLASS(I).EQ.6)THEN + WRITE(KBOUT,1005)CUTOFF(I) +1005 FORMAT(' Which is membership of a set with score',F8.3) + END IF + IF(COMBIN(I).EQ.'O')WRITE(KBOUT,1010) +1010 FORMAT(' It is orred with the previous motif.') + IF(COMBIN(I).EQ.'N')WRITE(KBOUT,1012) +1012 FORMAT(' It is notted with the previous motif.') + IF((COMBIN(I).EQ.'A').AND.(I.GT.1))WRITE(KBOUT,1009) +1009 FORMAT(' It is anded with the previous motif.') +100 CONTINUE + END +C********************************************************************* + SUBROUTINE SAVSIG( + + IDEV,STRING,MAXSTR, + + LENGTH,CLASS,RELMOT,RANGES,RANGEL, + + RANGET,RANGEM, + + STRNGS,NMOT,WEIGHT,MAXWTS,CUTOFF, + + WTSTR,RELEND,NAMSAV,COMBIN,KEYNS,IDEVW,FILET, + +KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,CHRSET,IDM,TITLEP) +C 18-7-91 Added pattern title + CHARACTER STRING(MAXSTR),FILET*(*) + INTEGER LENGTH(NMOT),CLASS(NMOT),RELMOT(NMOT),RELEND(NMOT) + INTEGER RANGES(NMOT),RANGEL(NMOT),RANGET(NMOT),RANGEM(NMOT) + INTEGER WTSTR(NMOT),STRNGS(NMOT) + REAL WEIGHT(MAXWTS),CUTOFF(NMOT) + CHARACTER COMBIN(NMOT),SCLASS*10,TITLE(60),NEWT(60) + CHARACTER HELPF*(*),CHRSET(IDM) + CHARACTER*(*) KEYNS(NMOT) + CHARACTER*(*) NAMSAV(NMOT),TITLEP + EXTERNAL NOTIRL +C +C +C SAVE THE SIGNAL +C + 1 CONTINUE + LIN = 0 + CALL GTSTR('Pattern title',' ',TITLEP,LIN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(INFLAG.EQ.2) RETURN + WRITE(IDEV,1000)TITLEP + 1000 FORMAT(' ',A) + DO 100 I = 1,NMOT + WRITE(SCLASS,1014)CLASS(I) +1014 FORMAT(I10) + DO 10 K = 1,10 + IF(SCLASS(K:K).NE.' ')THEN + J = 0 + DO 5 K1 = K,10 + J = J+1 + SCLASS(J:J) = SCLASS(K:K) + SCLASS(K:K) = ' ' +5 CONTINUE + END IF +10 CONTINUE + WRITE(IDEV,1001)COMBIN(I),SCLASS,KEYNS(I) +1001 FORMAT(' ',A,A,' ',A8,' Class ') + IF((I.NE.1).AND.(COMBIN(I).NE.'O'))THEN + WRITE(IDEV,1008)RELMOT(I) +1008 FORMAT(I7,' Relative motif') +1003 FORMAT(I7,' Relative start position') +1004 FORMAT(I7,' Number of extra positions') + WRITE(IDEV,1003)RANGES(I)+1 + WRITE(IDEV,1004)RANGEL(I) + END IF + IF(CLASS(I).EQ.1)THEN + WRITE(IDEV,1002) + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) +1002 FORMAT((' ',50A1)) + WRITE(IDEV,1006) +1006 FORMAT(' @ End of string') + ELSE IF(CLASS(I).EQ.2)THEN + WRITE(IDEV,1002) + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) + WRITE(IDEV,1006) + WRITE(IDEV,1009)CUTOFF(I) + ELSE IF(CLASS(I).EQ.3)THEN + WRITE(IDEV,1002) + + (STRING(K),K=STRNGS(I),STRNGS(I)+LENGTH(I)-1) + WRITE(IDEV,1006) + WRITE(IDEV,1009)CUTOFF(I) + ELSE IF(CLASS(I).EQ.4)THEN + WRITE(IDEV,1005)NAMSAV(I) +1005 FORMAT(A) +1010 FORMAT(I7,' Length') +1012 FORMAT(I7,' Minimum loop') +1013 FORMAT(I7,' Maximum loop') +1009 FORMAT(F10.5,' Cutoff') + ELSE IF(CLASS(I).EQ.5)THEN + WRITE(IDEV,1010)LENGTH(I) + WRITE(IDEV,1012)RANGET(I)-LENGTH(I)-1 + WRITE(IDEV,1013)RANGEM(I)-LENGTH(I)-1 + WRITE(IDEV,1009)CUTOFF(I) + ELSE IF(CLASS(I).EQ.6)THEN +C WRITE(*,*)NAMSAV(I) + IF(NAMSAV(I)(1:11).EQ.'FILENOTUSED') THEN + WRITE(KBOUT,1023)I +1023 FORMAT(' Motif',I3, + + ' needs a file name to store set as a weight matrix') + FILET = ' ' + CALL OPENF1(IDEVW,FILET,1,IOK,KBIN,KBOUT, + + 'Weight matrix file name', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + NAMSAV(I) = FILET + CALL FILLC(TITLE,60,' ') +20 CONTINUE + WRITE(KBOUT,1026) +1026 FORMAT(' Weight matrix needs a title') + L = 0 + CALL GETSTR('Title',TITLE,NEWT,60,L,KBOUT,KBIN,INFLAG) + IF(L.LT.0) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 20 + END IF + IF(INFLAG.EQ.2) RETURN + IF(L.GT.0)CALL SQCOPY(NEWT,TITLE,L) + MIDDLE = 0 + TOP = LENGTH(I) + CALL WRTSCN(TITLE,LENGTH(I),MIDDLE,CUTOFF(I),TOP, + + IDM,WEIGHT(WTSTR(I)),CHRSET, + + IDEVW) + END IF + WRITE(IDEV,1005)NAMSAV(I) + END IF +100 CONTINUE + END + SUBROUTINE GETMT1(STRING,MAXSTR,LENGTH,KBIN,KBOUT,IOK,JDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + CHARACTER NEW(50),STRING(MAXSTR) +C GETS DETAILS FOR MOTIF CLASS 1 + IOK = 1 + IF(KBIN.EQ.JDEV)THEN +10 CONTINUE + LENGTH = 0 + CALL GETSTR('String',STRING,NEW,50,LENGTH,KBOUT,KBIN,INFLAG) + IF(LENGTH.LT.1) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + CALL SQCOPY(NEW,STRING,LENGTH) + ELSE + LENGTH = MAXSTR + CALL ARRFIL(JDEV,STRING,LENGTH,KBOUT) + IF(LENGTH.LT.1)THEN + WRITE(KBOUT,*)'ZERO LENGTH STRING' + RETURN + END IF + END IF + IOK = 0 + RETURN + END +C********************************************************************* + SUBROUTINE GETMT2(STRING,MAXSTR,LENGTH,CUTOFF,KBIN,KBOUT,IOK,JDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + CHARACTER NEW(50),STRING(MAXSTR) + REAL MININ,MAXIN +C GETS DETAILS FOR MOTIF CLASS 2 + IOK = 1 + IF(KBIN.EQ.JDEV)THEN +10 CONTINUE + LENGTH = 0 + CALL GETSTR('String',STRING,NEW,50,LENGTH,KBOUT,KBIN,INFLAG) + IF(LENGTH.LT.1) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + CALL SQCOPY(NEW,STRING,LENGTH) + MININ = 1. + MAXIN = LENGTH + CUTOFF = LENGTH + CALL GETRL(MININ,MAXIN,CUTOFF,'Minimum matches', + + VALUE,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CUTOFF = VALUE + ELSE + LENGTH = MAXSTR + CALL ARRFIL(JDEV,STRING,LENGTH,KBOUT) + IF(LENGTH.LT.1)THEN + WRITE(KBOUT,*)'ZERO LENGTH STRING' + RETURN + END IF + READ(JDEV,1002,ERR=901)CUTOFF +1002 FORMAT(F10.0) + END IF + IOK = 0 + RETURN +901 CONTINUE + IOK = 1 + END +C********************************************************************* + SUBROUTINE GETMT3(STRING,MAXSTR,LENGTH,CUTOFF,MAT3,IDMAT3, + +KBIN,KBOUT,IOK,JDEV, + + IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + CHARACTER NEW(50),STRING(MAXSTR) + INTEGER MAT3(IDMAT3,IDMAT3) + REAL MININ,MAXIN +C GETS DETAILS FOR MOTIF CLASS 3 + IOK = 1 + IF(KBIN.EQ.JDEV)THEN +10 CONTINUE + LENGTH = 0 + CALL GETSTR('String',STRING,NEW,50,LENGTH,KBOUT,KBIN,INFLAG) + IF(LENGTH.LT.1) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + CALL SQCOPY(NEW,STRING,LENGTH) + CALL GTSCR(STRING,LENGTH,MAT3,IDMAT3,ISMIN,ISMAX) + MININ = ISMIN + MAXIN = ISMAX + CUTOFF = MAXIN + CALL GETRL(MININ,MAXIN,CUTOFF,'Minimum score', + + VALUE,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CUTOFF = VALUE + ELSE + LENGTH = MAXSTR + CALL ARRFIL(JDEV,STRING,LENGTH,KBOUT) + IF(LENGTH.LT.1)THEN + WRITE(KBOUT,*)'ZERO LENGTH STRING' + RETURN + END IF + READ(JDEV,1002,ERR=901)CUTOFF +1002 FORMAT(F10.0) + END IF + IOK = 0 + RETURN +901 CONTINUE + IOK = 1 + END + SUBROUTINE GETMT6(STRING,MAXSTR,LENGTH, + +WEIGHT,MAXWTS,CUTOFF,KBIN,KBOUT,JDEV,IDEV,IDM, + +FILNAM,IOK, + + IHELPS,IHELPE,HELPF,IDEVH,IPROB) + CHARACTER HELPF*(*) + PARAMETER (MAXS = 75) + CHARACTER STRING(MAXSTR),FILNAM*(*),NEW(MAXS) + REAL WEIGHT(MAXWTS),MININ,MAXIN + PARAMETER (MAXCHR = 26,MAXLEN = 120) + INTEGER SUM(MAXCHR,MAXLEN),TOT(MAXLEN) + PARAMETER (MAXPRM = 8) + CHARACTER PROMPT(2)*(MAXPRM) +C GETS DETAILS FOR MOTIF CLASS 6 + IOK = 1 + IOPT = 0 + DO 10 I = 1,MAXLEN + TOT(I) = 0 +10 CONTINUE +100 CONTINUE + IF(KBIN.EQ.JDEV)THEN + IOPT = 1 + PROMPT(1) = 'Keyboard' + PROMPT(2) = 'File' + CALL RADION('Select input mode',PROMPT,2,IOPT, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOPT.LT.1) RETURN + IF(IOPT.EQ.1)THEN +20 CONTINUE + WRITE(KBOUT,1000) +1000 FORMAT(' Separate sets with commas') + LENGTH = 0 + CALL GETSTR('String',STRING,NEW,MAXS,LENGTH,KBOUT,KBIN,INFLAG) + IF(LENGTH.LT.1) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 20 + END IF + IF(INFLAG.EQ.2) RETURN + CALL SQCOPY(NEW,STRING,LENGTH) + L = LENGTH + CALL INTRP6(STRING,L,LENGTH,SUM,MAXCHR,MAXLEN,CUTOFF,IOK) + IF(IOK.NE.0)RETURN + MININ = 1. + MAXIN = LENGTH +C CUTOFF = LENGTH + CALL GETRL(MININ,MAXIN,CUTOFF,'Minimum matches', + + VALUE,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CUTOFF = VALUE + ELSE + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + + 'Weight matrix file name', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + LENGTH=MAXLEN + CALL RDWMT(TOT,SUM,MIDDLE,LENGTH,MAXLEN,CUTOFF,YMAX,IDEV, + + IOK,IDM,KBOUT,IPROB) + IF(IOK.NE.0)THEN + WRITE(KBOUT,1006) +1006 FORMAT(' Error in weight matrix') + RETURN + END IF + END IF + ELSE + CALL OPENF(IDEV,FILNAM,0,IOK,JDEV,KBOUT) + IF(IOK.NE.0)RETURN + LENGTH=MAXLEN + CALL RDWMT(TOT,SUM,MIDDLE,LENGTH,MAXLEN,CUTOFF,YMAX,IDEV, + + IOK,IDM,KBOUT,IPROB) + IF(IOK.NE.0)THEN + WRITE(KBOUT,1006) + RETURN + END IF + END IF + CALL GETW1(SUM,WEIGHT,LENGTH,MAXCHR,MAXLEN) + END + SUBROUTINE GETMT4(WEIGHT,MAXWTS,LENGTH,CUTOFF,YMAX,IDEV, + +KBIN,KBOUT,IOK,JDEV,FILNAM,IDM, + + IHELPS,IHELPE,HELPF,IDEVH,IPROB) + CHARACTER HELPF*(*) + PARAMETER (MAXLEN = 120, MAXCHR = 26) + REAL WEIGHT(MAXWTS) + CHARACTER FILNAM*(*) + INTEGER TOT(MAXLEN),SUM(MAXCHR,MAXLEN) + IF(JDEV.EQ.KBIN) THEN + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,JDEV,KBOUT, + + 'Weight matrix file name', + + IHELPS,IHELPE,HELPF,IDEVH) + ELSE + CALL OPENF(IDEV,FILNAM,0,IOK,JDEV,KBOUT) + END IF + IF(IOK.NE.0)RETURN + LENGTH=MAXLEN + CALL RDWMT(TOT,SUM,MIDDLE,LENGTH,MAXLEN,CUTOFF,YMAX,IDEV, + + IOK,IDM,KBOUT,IPROB) + IF(IOK.NE.0)THEN + WRITE(KBOUT,*)' Error in weight matrix, option left' + RETURN + END IF + IF(CUTOFF.LT.0.0)CALL GETW(TOT,SUM,WEIGHT,LENGTH,IDM,MAXLEN) + IF(CUTOFF.GE.0.0)CALL GETW2(SUM,WEIGHT,LENGTH,IDM,MAXLEN) + END +C********************************************************************* + SUBROUTINE INTRP6(STRING,ISEND,LENGTH,WT,MAXCHR,MAXLEN, + +CUTOFF,IOK) + CHARACTER STRING(ISEND),TERM + INTEGER WT(MAXCHR,MAXLEN) + INTEGER CTONUM + EXTERNAL CTONUM + PARAMETER (TERM = ',') + IOK = 0 +C POINT TO STRING + IS = 1 + ICOL = 1 +C COUNT FILLED COLUMNS + CUTOFF = 0. +10 CONTINUE + CALL FILLI(WT(1,ICOL),MAXCHR,0) + CUTOFF = CUTOFF + 1. +20 CONTINUE + IF(IS.LE.ISEND)THEN + IF(STRING(IS).NE.TERM)THEN + IROW = CTONUM(STRING(IS)) + WT(IROW,ICOL) = 1 + IS = IS + 1 + GO TO 20 + END IF + NC = 1 +30 CONTINUE + IF(IS.LE.ISEND)THEN + IF(STRING(IS).EQ.TERM)THEN + ICOL = ICOL + 1 + CALL FILLI(WT(1,ICOL),MAXCHR,0) + NC = NC + 1 + IS = IS + 1 + GO TO 30 + END IF +C +C END OF TERMINATORS +C + IF(ICOL.GT.1) GO TO 10 +C ERROR TERMINATOR BEFORE ANY GOOD COLUMNS + IOK = 1 + RETURN + END IF +C STRING ENDED WITH TERMINATOR + LENGTH = ICOL - NC + 1 + RETURN + END IF +C +C END REACHED WITH NO TERMINATOR (NORMAL) + IF(ICOL.GT.0)THEN + LENGTH = ICOL + IOK = 0 + RETURN + END IF + IOK = 1 + END + SUBROUTINE GETMT8(RANGES,RANGEL,LENGTH,CUTOFF, + +KBIN,KBOUT,JDEV,IOK, + + IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER HELPF*(*) + INTEGER RANGES,RANGEL +C GETS DETAILS FOR MOTIF CLASS 8 + IF(JDEV.EQ.KBIN)THEN + IOK = 1 + MININ = 1 + MAXIN = 60 + LENGTH = 6 + CALL GETINT(MININ,MAXIN,LENGTH,'Repeat length',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + LENGTH = IVAL + MININ = 0 + MAXIN = 60 + ID = MININ + CALL GETINT(MININ,MAXIN,ID,'Minimum gap', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + ID = IVAL + MININ = ID + MAXIN = MININ + 60 + IR = ID + CALL GETINT(MININ,MAXIN,IR,'Maximum gap', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IR = IVAL +C FUDGE HERE: SHOULD REALLY LOOK AT SEQUENCE COMPOSITION +C AND SCORE MATRIX VALUES. ASSUMING MDM78 + SMININ = 11.0 + SMAXIN = LENGTH*20. + CUTOFF = LENGTH * 12. + CALL GETRL(SMININ,SMAXIN,CUTOFF,'Minimum score', + + VALUE,KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + CUTOFF = VALUE + ELSE + READ(JDEV,1001,ERR=901)LENGTH + IF(LENGTH.LT.1)RETURN + READ(JDEV,1001,ERR=901)ID +1001 FORMAT(I7) +C IF(ID.LT.0)GO TO 220 + READ(JDEV,1001,ERR=901)IR + READ(JDEV,1006,ERR=901)CUTOFF +1006 FORMAT(F10.0) + END IF + RANGES = ID + LENGTH + 1 + RANGEL = IR + LENGTH + 1 + IOK = 0 + RETURN +901 CONTINUE + IOK = 1 + END + SUBROUTINE WRTSCN(TITLE,LENGTH,MIDDLE,BOT,TOP,IDM, + +SUM,CHRSET,IDEV) + INTEGER TOT(120) + REAL SUM(IDM,LENGTH) + CHARACTER CHRSET(IDM),TITLE(60) +C PROTEIN MATRICES DONT WRITE ROWS FOR -X? AND SPACE SO SET DIMENSION +C TO IDM-4 + CALL FILLI(TOT,120,0) + WRITE(IDEV,1018)TITLE +1018 FORMAT(' ',60A1) +1019 FORMAT(' P',20I4) +1020 FORMAT(' N',20I4) +1021 FORMAT(' ',A,20I4) +1022 FORMAT(' ',2I6,2F10.3) + WRITE(IDEV,1022)LENGTH,MIDDLE,BOT,TOP + NLINES=1+(LENGTH-1)/20 + K1=1 + DO 400 J=1,NLINES + K2=MIN((K1+19),LENGTH) + WRITE(IDEV,1019)(K,K=K1-MIDDLE,K2-MIDDLE) + WRITE(IDEV,1020)(TOT(K),K=K1,K2) + DO 390 I=1,IDM-4 + WRITE(IDEV,1021)CHRSET(I),(INT(SUM(I,K)),K=K1,K2) +390 CONTINUE + K1=K1+20 + IF(K1.GT.LENGTH)K1=LENGTH +400 CONTINUE + CLOSE(UNIT=IDEV) + END + SUBROUTINE GETCLS(JDEV,KBOUT,ICLASS,TCLASS,KEYNAM) + CHARACTER TCLASS,STRING*21,NUMBER*10,STRNG2*10,KEYNAM*(*) + SAVE NUMBER + DATA NUMBER/'0987654321'/ + ICLASS = 0 +10 CONTINUE + STRING = ' ' + READ(JDEV,1000,ERR=10,END=70)STRING +1000 FORMAT(A) + KEYNAM = STRING(14:21) + DO 20 I = 10,1,-1 + K = I + IF(STRING(I:I).NE.' ')GO TO 21 +20 CONTINUE + RETURN +21 CONTINUE + L = 1 + DO 30 I = 1,K + L = I + IF(STRING(I:I).NE.' ')GO TO 31 +30 CONTINUE +31 CONTINUE + TCLASS = STRING(L:L) + DO 40 I = 1,10 + IF(TCLASS.EQ.NUMBER(I:I))THEN + TCLASS = 'A' + GO TO 41 + END IF +40 CONTINUE + IF((TCLASS.NE.'O').AND.(TCLASS.NE.'N').AND.(TCLASS.NE.'A') + +.AND.(TCLASS.NE.'-'))THEN + ICLASS = 99999 + RETURN + END IF + L = L + 1 +41 CONTINUE + J = 11 + STRNG2 = ' ' + DO 50 I = K,L,-1 + J = J-1 + STRNG2(J:J) = STRING(I:I) +50 CONTINUE + READ(STRNG2,1001,ERR=60)ICLASS +1001 FORMAT(I10) + IF(TCLASS.EQ.'-')ICLASS=-1*ICLASS + RETURN +60 CONTINUE + ICLASS = 99999 + RETURN +70 CONTINUE + END + SUBROUTINE GETP(ICLASS,STRING,LENGTH,IDM, + +MATRIX,MAT1,WEIGHT) +C MAT1 SIMPLE IDENTITY +C MATRIX MDM78 + PARAMETER ( + + MAXCHR=26, + + MAXSIG=120) + INTEGER MAT1(IDM,IDM),MATRIX(IDM,IDM) + INTEGER IWT(MAXCHR,MAXSIG) + REAL WEIGHT(IDM,LENGTH),FB(MAXCHR),F(MAXCHR) + CHARACTER STRING(LENGTH) + COMMON /COMPC/COMP(MAXCHR) + DO 10 I = 1,IDM + F(I) = COMP(I) +10 CONTINUE + IF(ICLASS.EQ.1)THEN + CALL GETP1(STRING,LENGTH,IDM,MAT1,IDM,IWT,F) + RETURN + END IF + IF(ICLASS.EQ.2)THEN + CALL GETP1(STRING,LENGTH,IDM,MAT1,IDM,IWT,F) + RETURN + END IF + IF(ICLASS.EQ.3)THEN + CALL GETP3(STRING,LENGTH,IDM,MATRIX,IWT,IDM,F) + RETURN + END IF + IF(ICLASS.EQ.4)THEN + CALL GETP4(WEIGHT,IWT,IDM,LENGTH,F) + RETURN + END IF + IF(ICLASS.EQ.5)THEN + CALL GETP8(MATRIX,IDM,IWT,IDM,LENGTH,F,FB) + RETURN + END IF + IF(ICLASS.EQ.6)THEN + CALL GETP4(WEIGHT,IWT,IDM,LENGTH,F) + RETURN + END IF + END + SUBROUTINE WTGEN(IWT,MAXCHR,LENGTH,F,MAXS,SMIN,SCALE,JOB) + PARAMETER (MAXPOL = 4000) + INTEGER IWT(MAXCHR,LENGTH) + REAL F(MAXCHR) + COMMON /POLY/POLYA(0:MAXPOL),POLYB(0:MAXPOL),POLYC(0:MAXPOL), + +CMIN,CSCALE,LENTHC + SAVE /POLY/ + CMIN = SMIN + CSCALE = SCALE + LENTHC = LENGTH + DO 10 I = 0, MAXPOL + POLYA(I) = 0.0 +10 CONTINUE + DO 400 I = 1,MAXCHR + K = IWT(I,1) + POLYA(K) = POLYA(K) + F(I) +400 CONTINUE + MAXS1 = MAXS + DO 600 J = 2,LENGTH + DO 490 I = 0, MAXPOL + POLYB(I) = 0.0 +490 CONTINUE + DO 500 I = 1,MAXCHR + K = IWT(I,J) + POLYB(K) = POLYB(K) + F(I) +500 CONTINUE + CALL POLMUL(MAXS1,MAXS,MAXSP1) + MAXS1 = MAXSP1 +600 CONTINUE +C IF JOB = 1 WANT DISTRIBUTION ONLY +C JOB = 2 WANT CUMMULATIVE VALUES +C JOB = 3 WANT DISTRIBUTION AND UNSCALED SCORES +C JOB = 4 WANT CUMMULATIVE VALUES AND UNSCALED SCORES + IF((JOB.EQ.2).OR.(JOB.EQ.4))THEN + DO 610 I = 1,MAXS1 + J = MAXS1 - I + POLYA(J) = POLYA(J) + POLYA(J+1) +610 CONTINUE + END IF + IF((JOB.EQ.3).OR.(JOB.EQ.4))THEN + T = SMIN * LENGTH + DO 620 I = 0,MAXS1 + POLYB(I) = (REAL(I)/SCALE) + T +620 CONTINUE + END IF + END + SUBROUTINE SCGEN(IWT,MAXCHR,LENGTH,FA,FB,MAXS, + +SCALE,SMIN,JOB) + PARAMETER (MAXPOL = 4000) + INTEGER IWT(MAXCHR,MAXCHR) + REAL FA(MAXCHR),FB(MAXCHR) + COMMON /POLY/POLYA(0:MAXPOL),POLYB(0:MAXPOL),POLYC(0:MAXPOL), + +CMIN,CSCALE,LENTHC + SAVE /POLY/ + CMIN = SMIN + CSCALE = SCALE + LENTHC = LENGTH + DO 10 I = 0, MAXPOL + POLYA(I) = 0.0 + POLYB(I) = 0.0 +10 CONTINUE + DO 400 I = 1,MAXCHR + DO 300 J = 1,MAXCHR + K = IWT(I,J) + POLYA(K) = POLYA(K) + FA(I) * FB(J) + POLYB(K) = POLYA(K) +300 CONTINUE +400 CONTINUE + MAXS1 = MAXS + DO 600 J = 2,LENGTH + CALL POLMUL(MAXS1,MAXS,MAXSP1) + MAXS1 = MAXSP1 +600 CONTINUE +C IF JOB = 1 WANT DISTRIBUTION ONLY +C JOB = 2 WANT CUMMULATIVE VALUES +C JOB = 3 WANT DISTRIBUTION AND UNSCALED SCORES +C JOB = 4 WANT CUMMULATIVE VALUES AND UNSCALED SCORES + IF((JOB.EQ.2).OR.(JOB.EQ.4))THEN + DO 610 I = 1,MAXS1 + J = MAXS1 - I + POLYA(J) = POLYA(J) + POLYA(J+1) +610 CONTINUE + END IF + IF((JOB.EQ.3).OR.(JOB.EQ.4))THEN + T = SMIN * LENGTH + DO 620 I = 0,MAXS1 + POLYB(I) = (REAL(I)/SCALE) + T +620 CONTINUE + END IF + END + SUBROUTINE SCGEN1(IWT,MAXCHR,LENGTH,FA,MAXS, + +SCALE,SMIN,JOB,STRING) +C AUTHOR RODGER STADEN +C PROBABILITY FOR STRINGS + CHARACTER STRING(LENGTH) + INTEGER DTONUM + PARAMETER (MAXPOL = 4000) + INTEGER IWT(MAXCHR,MAXCHR) + REAL FA(MAXCHR) + COMMON /POLY/POLYA(0:MAXPOL),POLYB(0:MAXPOL),POLYC(0:MAXPOL), + +CMIN,CSCALE,LENTHC + EXTERNAL DTONUM + SAVE /POLY/ + CMIN = SMIN + CSCALE = SCALE + LENTHC = LENGTH + DO 10 I = 0, MAXPOL + POLYA(I) = 0.0 +10 CONTINUE + K1 = DTONUM(STRING(1)) + DO 400 I = 1,MAXCHR + K = IWT(I,K1) + POLYA(K) = POLYA(K) + FA(I) +400 CONTINUE + MAXS1 = MAXS + DO 600 J = 2,LENGTH + DO 490 I = 0,MAXPOL + POLYB(I) = 0. +490 CONTINUE + K1 = DTONUM(STRING(J)) + DO 500 I = 1,MAXCHR + K = IWT(I,K1) + POLYB(K) = POLYB(K) + FA(I) +500 CONTINUE + CALL POLMUL(MAXS1,MAXS,MAXSP1) + MAXS1 = MAXSP1 +600 CONTINUE +C IF JOB = 1 WANT DISTRIBUTION ONLY +C JOB = 2 WANT CUMMULATIVE VALUES +C JOB = 3 WANT DISTRIBUTION AND UNSCALED SCORES +C JOB = 4 WANT CUMMULATIVE VALUES AND UNSCALED SCORES + IF((JOB.EQ.2).OR.(JOB.EQ.4))THEN + DO 610 I = 1,MAXS1 + J = MAXS1 - I + POLYA(J) = POLYA(J) + POLYA(J+1) +610 CONTINUE + END IF + IF((JOB.EQ.3).OR.(JOB.EQ.4))THEN + T = SMIN * LENGTH + DO 620 I = 0,MAXS1 + POLYB(I) = (REAL(I)/SCALE) + T +620 CONTINUE + END IF + END + SUBROUTINE POLMUL(NA,NB,NC) + PARAMETER (MAXPOL = 4000) + COMMON /POLY/POLYA(0:MAXPOL),POLYB(0:MAXPOL),POLYC(0:MAXPOL), + +CMIN,CSCALE,LENTHC + PARAMETER (ZERO=0.0,SMALL=1E-10) + SAVE /POLY/ +C POLYA, POLYB ARE INPUT POLYNOMIAL COEEFICIENTS +C POLYC IS OUTPUT POLYNOMIAL COEEFICIENTS +C + NC = NA + NB + DO 210 I = 0,MAXPOL + POLYC(I) = ZERO +210 CONTINUE + IF(NC.GT.MAXPOL)RETURN + DO 230 I = 0,NA + DO 220 J = 0,NB + POLYC(I+J) = POLYC(I+J) + POLYA(I) * POLYB(J) +220 CONTINUE +230 CONTINUE + DO 240 I = 0,NC + T = POLYC(I) + IF(T.LT.SMALL) T = ZERO + POLYA(I) = T +240 CONTINUE + END + SUBROUTINE WRTWMT(WEIGHT,IWT,MAXCHR,LENGTH,IDEV,IFLAG) + REAL WEIGHT(MAXCHR,LENGTH) + INTEGER IWT(MAXCHR,LENGTH) + DO 10 I = 1,MAXCHR + IF(IFLAG.EQ.0) + + WRITE(IDEV,1000,ERR=100)(WEIGHT(I,J),J=1,LENGTH) + IF(IFLAG.EQ.1) + + WRITE(IDEV,1001,ERR=100)(IWT(I,J),J=1,LENGTH) +10 CONTINUE +1000 FORMAT(' ',20F5.2) +1001 FORMAT(' ',20I4) +100 CONTINUE + END + SUBROUTINE WTSC(WEIGHT,IWT,MAXCHR,LENGTH,MAXS,SCALE,SMIN) + INTEGER IWT(MAXCHR,LENGTH) + REAL WEIGHT(MAXCHR,LENGTH) + PARAMETER (SMALL=1E-10) + PARAMETER (MAXPOL = 4000) + SMIN = 9999999.9 + SMAX = -99999999.9 + DO 100 J = 1,LENGTH + DO 90 I = 1,MAXCHR + T = WEIGHT(I,J) + IF(T.LT.SMIN)SMIN = T + IF(T.GT.SMAX)SMAX = T +90 CONTINUE +100 CONTINUE + SMAX = SMAX - SMIN + IF(SMAX.EQ.0.0)SMAX = SMALL +C USE LENGTH+1 TO DIVIDE MAXPOL. THIS IS AN ATTEMPT TO AVOID +C THE USE OF NINT GIVING A SET OF SCORES THAT SUM TO TOO +C HIGH A VALUE (IE GT MAXPOL) + MAXS = MAXPOL/(LENGTH+1) + MAXS = MIN(MAXS,40) + SCALE = REAL(MAXS)/SMAX + DO 200 J = 1,LENGTH + DO 190 I = 1,MAXCHR + K = NINT((WEIGHT(I,J)-SMIN)*SCALE) + IWT(I,J) = K +190 CONTINUE +200 CONTINUE + END + SUBROUTINE GETCMP(SEQ,IDIM,COMP,IDM) + CHARACTER SEQ(IDIM) + REAL COMP(IDM) + INTEGER DTONUM + EXTERNAL DTONUM + DO 10 I = 1,IDM + COMP(I) = 0.0 +10 CONTINUE + T = 0. + DO 20 I = 1,IDIM + J = DTONUM(SEQ(I)) + IF(J.LT.23)THEN + COMP(J) = COMP(J) + 1. + T = T + 1. + END IF +20 CONTINUE + IF(T.GT.0.0)THEN + DO 30 I = 1,IDM + COMP(I) = COMP(I) / T +30 CONTINUE + END IF + END + SUBROUTINE MATSC(WEIGHT,IDMAT,IWT,MAXCHR,LENGTH,MAXS, + +SMIN,SCALE) + INTEGER IWT(IDMAT,IDMAT) + INTEGER WEIGHT(IDMAT,IDMAT) + PARAMETER (SMALL=1E-10) + PARAMETER (MAXPOL = 4000) + ISMIN = 99999999 + ISMAX = -99999999 + DO 100 J = 1,IDMAT + DO 90 I = 1,IDMAT + K = WEIGHT(I,J) + IF(K.LT.ISMIN) ISMIN = K + IF(K.GT.ISMAX) ISMAX = K +90 CONTINUE +100 CONTINUE +C SMAX = ISMAX - ISMIN +C IF(SMAX.EQ.0.0)SMAX = SMALL +C DO NOTHING HERE - ASSUME VALUES ARE IN RANGE AND JUST COPY +C USE LENGTH+1 TO DIVIDE MAXPOL. THIS IS AN ATTEMPT TO AVOID +C THE USE OF NINT GIVING A SET OF SCORES THAT SUM TO TOO +C HIGH A VALUE (IE GT MAXPOL) +C MAXS = MAXPOL/(LENGTH+1) +C SCALE = REAL(MAXS)/SMAX + DO 200 J = 1,IDMAT + DO 190 I = 1,IDMAT +C K = NINT((REAL(WEIGHT(I,J))-ISMIN)*SCALE) + IWT(I,J) = WEIGHT(I,J) - ISMIN +190 CONTINUE +200 CONTINUE + SCALE = 1.0 + SMIN = ISMIN + MAXS = ISMAX + END + REAL FUNCTION PSCORE(SCORE) + PARAMETER (MAXPOL = 4000) + COMMON /POLY/POLYA(0:MAXPOL),POLYB(0:MAXPOL),POLYC(0:MAXPOL), + +SMIN,SCALE,LENGTH + SAVE /POLY/ +C WANT PROBABILITY OF SCORING AT LEAST SCORE +C CUMMULATIVE PROBABILITIES IN CUMP +C SCALE FACTORS SMIN AND SCALE AND MOTIF LENGTH IS LENGTH + I = NINT((SCORE - SMIN * LENGTH) * SCALE) + PSCORE = -1. + IF((I.GE.0).AND.(I.LE.MAXPOL)) PSCORE = POLYA(I) + END + SUBROUTINE GETP1(STRING,LENGTH,IDMAT3,MAT2,IDMAT2,IWT,F) + INTEGER MAT2(IDMAT2,IDMAT2) + INTEGER IWT(IDMAT2,IDMAT2) + REAL F(IDMAT3) + CHARACTER STRING(LENGTH) + JOB = 2 + CALL MATSC(MAT2,IDMAT2,IWT,IDMAT3,LENGTH,MAXS,SMIN,SCALE) + CALL SCGEN1(IWT,IDMAT2,LENGTH,F,MAXS,SCALE,SMIN,JOB,STRING) + END + SUBROUTINE GETP3(STRING,LENGTH,IDMAT3,MAT3,IWT,IDM,F) + INTEGER MAT3(IDMAT3,IDMAT3) + INTEGER IWT(IDMAT3,IDMAT3) + REAL F(IDMAT3) + CHARACTER STRING(LENGTH) + JOB = 2 + CALL MATSC(MAT3,IDMAT3,IWT,IDM,LENGTH,MAXS,SMIN,SCALE) + CALL SCGEN1(IWT,IDMAT3,LENGTH,F,MAXS,SCALE,SMIN,JOB, + + STRING) + END + SUBROUTINE GETP4(WEIGHT,IWT,IDM,LENGTH,F) + REAL F(IDM) + REAL WEIGHT(IDM,LENGTH) + INTEGER IWT(IDM,LENGTH) + JOB = 2 + CALL WTSC(WEIGHT,IWT,IDM,LENGTH,MAXS,SCALE,SMIN) + CALL WTGEN(IWT,IDM,LENGTH,F,MAXS,SMIN,SCALE,JOB) + END + SUBROUTINE GETP8(MAT1,IDMAT1,IWT,IDM,LENGTH,F,FB) + REAL F(IDM),FB(IDM) + INTEGER MAT1(IDMAT1,IDMAT1) + INTEGER IWT(IDM,LENGTH) + DO 30 I = 1,IDM + FB(I) = F(I) +30 CONTINUE + JOB = 2 + CALL MATSC(MAT1,IDMAT1,IWT,IDM,LENGTH,MAXS,SMIN,SCALE) + CALL SCGEN(IWT,IDMAT1,LENGTH,F,FB,MAXS,SCALE,SMIN,JOB) + END + SUBROUTINE WRTSCR(SCMIN,PROB,KBOUT) + WRITE(KBOUT,1000)SCMIN,PROB +1000 FORMAT(' Probability of score ',F10.4,' =',E10.3) + END + SUBROUTINE GIDMAT(MAT,IDM,IDMX) + INTEGER MAT(IDM,IDM) +C AUTHOR RODGER STADEN +C ALLOW ONLY - AS WILD CARD FOR PROTEINS + DO 10 I = 1,IDM + DO 5 J = 1,IDM + MAT(I,J) = 0 +5 CONTINUE +10 CONTINUE + DO 20 I = 1,IDMX+1 + DO 15 J = 1,IDMX+1 + IF(I.EQ.J)MAT(I,J) = 1 +15 CONTINUE +20 CONTINUE + DO 30 I = 1,IDMX + MAT(I,IDMX+1) = 1 + MAT(IDMX+1,I) = 1 +30 CONTINUE + END + SUBROUTINE FORWAD(CLASS,LENGTH,CUTOFF,NMOT, + +MOTIF,ICLASS,ILEN,CUT,IWT,RELMOT,START,IEND, + +RANGES,RANGEL,STRNGS,ISTRST,WTSTR,IDSEQ,IENTRY, + +RANGET,RANGEM,START2,IEND2,MATCHQ,RELEND,MATCHP,IDSPLY, + +COMBIN,COMB) + INTEGER CLASS(NMOT),LENGTH(NMOT),WTSTR(NMOT) + INTEGER STRNGS(NMOT),START(NMOT),IEND(NMOT),IENTRY(NMOT) + INTEGER RELMOT(NMOT),RANGES(NMOT),RANGEL(NMOT) + INTEGER RANGET(NMOT),RANGEM(NMOT),START2(NMOT),IEND2(NMOT) + INTEGER MATCHQ(NMOT),RELEND(NMOT),MATCHP(NMOT) + REAL CUTOFF(NMOT) + CHARACTER COMBIN(NMOT),COMB +C WRITE(*,*)'IN FORWAD FOR MOTIF',MOTIF +C ROUTINE TO MOVE FORWARDS ONE MOTIF +C FIRST IT UPDATES THE RANGES FOR ALL THE MOTIFS THAT DEPEND +C ON THE CURRENT MOTIF +C IT POINTS TO THE NEXT MOTIF AND GETS ALL THE REQUIRED VARIABLES +C WE MUST FIRST LOOK BACK THRU THE LIST UNTIL WE FIND THE FIRST +C NON ORED MOTIF. THEN WE MUST SET THE RELATIVE POSITIONS FOR ALL +C THOSE MOTIFS THAT DEPEND ON THIS SET OF ORED MOTIFS. THE RELATIVE +C POSITIONS MUST BE SET RELATIVE TO THE POSITION OF THE MATCH FOUND +C FOR THE CURRENT MOTIF EVEN IF IT IS NOT THE FIRST IN THE LIST OF ORS +C THIS IS BECAUSE RELATIVE POSITIONS CAN ONLY BE DEFINED RELATIVE TO +C THE FIRST OF A SET OF ORS, BUT IF A MATCH IS FOUND FOR ANY OF THE OTHERS +C IN THE SET THEN ITS POSITION DEFINES THE RANGE. +C +C +C +C IF THIS IS THE LAST MOTIF RETURN TO DISPLAY THE MATCH + IF(MOTIF.EQ.NMOT)THEN + IDSPLY = 1 + RETURN + END IF +C +C NOT THE LAST +C + IDSPLY = 0 +C +C IF THIS IS A NOTTED MOTIF THEN IT WILL NOT BE ORED OR HAVE ANY +C OTHER MOTIFS RELATIVE TO IT, SO DEAL WITH IT HERE +C + IF(COMB.EQ.'N')THEN + MOTIF = MOTIF + 1 + ICLASS = CLASS(MOTIF) + ILEN = LENGTH(MOTIF) + CUT = CUTOFF(MOTIF) + IWT = WTSTR(MOTIF) + ISTRST = STRNGS(MOTIF) + COMB = COMBIN(MOTIF) + RETURN + END IF +C +C +C NOW LOOK FOR THE FIRST NON ORRED CLASS FROM HERE BACK +C + NOTIF = MOTIF +10 CONTINUE +C + IF(COMBIN(NOTIF).EQ.'O')THEN + NOTIF = NOTIF - 1 + GO TO 10 + END IF +C +C NOTIF IS A NON-ORED MOTIF, OR THE FIRST OF A LIST OF ORS +C SO SET RELATIVE POSITIONS FOR ALL THOSE THAT DEPEND ON IT +C + DO 20 I = MOTIF+1,NMOT + JMOT = I + IF(RELMOT(JMOT).EQ.NOTIF)THEN + START(JMOT) = START(MOTIF) + RANGES(JMOT) - 1 +C NEXT LINE IS FOR RAH BUG + START(JMOT) = MAX(START(JMOT),1) + IEND(JMOT) = START(JMOT) + RANGEL(JMOT) - 1 + IF(IEND(JMOT).GT.IDSEQ)IEND(JMOT)=IDSEQ +C SET FLAG TO SAY THAT FOR STEMS WE DO NOT HAVE TO CONTINUE A PREVIOUS +C 5' STEM START POSITION (IE TRY ALL ITS REMAINING LOOPS) + IENTRY(JMOT) = 0 + END IF +C +20 CONTINUE +C +C +C OK WEVE FOUND THE FIRST NON-ORRED CLASS AND RESET RANGES +C RANGES ACCORDINGLY. NOW WE HAVE TO GO FORARDS THRU THE LIST UNTIL +C WE FIND THE NEXT NON-ORRED CLASS. IF WE SET THIS TO BE THE +C CURRENT MOTIF WE WILL HAVE MOVED FORWARDS. WE MUST BE CAREFUL THAT +C WE DONT FALL OFF THE END OF THE LIST! +C + JMOT = MOTIF +30 CONTINUE + JMOT = JMOT + 1 + IF(JMOT.GT.NMOT)THEN + IDSPLY = 1 + RETURN + END IF + IF(COMBIN(JMOT).EQ.'O')GO TO 30 + MOTIF = JMOT + ICLASS = CLASS(MOTIF) + ILEN = LENGTH(MOTIF) + CUT = CUTOFF(MOTIF) + IWT = WTSTR(MOTIF) + ISTRST = STRNGS(MOTIF) + COMB = COMBIN(MOTIF) + END +C********************************************************************* + SUBROUTINE BAKSID(CLASS,LENGTH,CUTOFF,STRNGS,NMOT, + +MOTIF,ICLASS,ILEN,CUT,IWT,ISTRST,WTSTR, + +RELMOT,START,IEND,MATCHQ,RANGES,RANGEL,RELEND,IRET,MATCHP, + +COMBIN,COMB) + INTEGER CLASS(NMOT),LENGTH(NMOT),WTSTR(NMOT) + INTEGER STRNGS(NMOT),RELMOT(NMOT),START(NMOT),IEND(NMOT) + INTEGER MATCHQ(NMOT),RANGES(NMOT),RANGEL(NMOT),RELEND(NMOT) + INTEGER MATCHP(NMOT) + REAL CUTOFF(NMOT) + CHARACTER COMBIN(NMOT),COMB +C WRITE(*,*)'IN BAKSID FOR MOTIF',MOTIF +C ROUTINE TO MOVE BAKWARDS OR SIDEWAYS +C IE WE COME HERE AFTER FAILING TO FIND A MATCH FOR THE PREVIOUS MOTIF +C WE CHECK IF WE CAN MOVE SIDEWAYS BY LOOKING TO SEE IF THE NEXT MOTIF +C IN THE LIST IS ORRED. +C IF IT IS WE LEAVE THE CURRENT MOTIFS POSITION UNCHANGED SO THAT IT +C IS READY TO SIGNIFIY TO A SUBSEQUENT MOVE BACKWARDS THAT ALL ITS +C POSITIONS HAVE BEEN TRIED FOR THE MATCHES FOUND FURTHER UP THE LIST. +C +C IF THE NEXT MOTIF IN THE LIST IS NOT ORED THEN WE MUST MOVE BACKWARDS. +C BEFORE DOING SO WE MUST RESET THE RANGES FOR ALL THE MOTIFS IN THE +C CURRENT SET OF ORS. THEY MUST BE RESET TO THEIR ORIGINAL FULL RANGE +C BECAUSE WE ARE TRYING TO FIND ALL POSSIBLE COMBINATIONS OF MATCH AND +C WHEN WE GO BACK WE MIGHT NOT GO BACK AS FAR AS THE MOTIF THE CURRENT +C SET DEPEND ON, AND SO THEY WILL NOT BE RESET COMING FORWARDS. SO IF WE +C FIND ANOTHER MATCH TO A MOTIF IN A PREVIOUS SET WE MUST ALLOW THE SAME +C MATCHES TO BE REFOUND IN THE CURRENT SET BECAUSE IT GIVES A DIFFERENT +C COMBINATION TO THOSE ALREADY FOUND. TO FIND THE ONES TO RESET WE MUST +C MOVE BACK THRU THE LIST UNTIL A NON-ORRED CLASS IS FOUND: THIS +C IS THE LAST ONE TO RESET. +C HAVING DONE THIS, TO MOVE BACK WE MUST LOOK BACK FROM THE LAST ONE +C RESET, UNTIL WE FIND THE FIRST NON-ORRED CLASS. THIS WILL +C BE AN ANDED OR NOTTED MOTIF OR THE FIRST IN A LIST OF ORS. +C WE MUST ALWAYS GO BACK +C TO THE BEGINNING OF A LIST OF ORS. IF THEY HAVE NO FURTHER POSITIONS TO +C TRY WE CAN THEN GO SIDEWAYS UNTIL EITHER ONE WITH SOME PLACES TO TRY +C IS FOUND, OR WE HAVE TO GIVE UP AND GO BACKWARDS. +C MOTIF 1 AND ANY MOTIFS ORED WITH IT ARE SPECIAL CASES FOR THE ROUTINES +C BECAUSE THEIR RANGES MUST NOT BE RESET. THEIR SPECIALNESS IS SIGNIFIED +C BY THEIR HAVING A ZERO RELATIVE MOTIF NUMBER. ALSO IF WE TRY TO GO BACK +C FOR MOTIF 1, WE MUST HAVE FINISHED TRYING ALL POSSIBLE COMBINATIONS, +C SO WE ESCAPE. +C +C +C +C RESET ITS MATCH POSITION TO ZERO AS A FLAG TO THE DISPLAY ROUTINES +C +C + MATCHP(MOTIF) = 0 +C +C + IF(MOTIF.LT.NMOT)THEN + IF(COMBIN(MOTIF+1).EQ.'O')THEN +C +C MOVE SIDEWAYS +C + MOTIF = MOTIF + 1 + ICLASS = CLASS(MOTIF) + ILEN = LENGTH(MOTIF) + CUT = CUTOFF(MOTIF) + IWT = WTSTR(MOTIF) + ISTRST = STRNGS(MOTIF) + MATCHP(MOTIF) = 0 + COMB = COMBIN(MOTIF) + RETURN + END IF + END IF +C +C +C +C WE MUST MOVE BACKWARDS. RESET RANGE FOR CURRENT MOTIF IF IT IS NOT +C NOTTED +C +C + IF(COMB.NE.'N')THEN +C +C + IREL = RELMOT(MOTIF) + IF(IREL.NE.0)THEN + START(MOTIF) = START(IREL) + RANGES(MOTIF) +C NEXT LINE FOR RAH BUG + START(MOTIF) = MAX(START(MOTIF),1) + IEND(MOTIF) = START(MOTIF) + RANGEL(MOTIF) - 1 + END IF +C +C +C +10 CONTINUE +C +C + IF(MOTIF.EQ.1)THEN + IRET = 1 + RETURN + END IF + IF(COMBIN(MOTIF).EQ.'O')THEN + MOTIF = MOTIF - 1 + MATCHP(MOTIF) = 0 + IREL = RELMOT(MOTIF) + IF(IREL.NE.0)THEN + START(MOTIF) = START(IREL) + RANGES(MOTIF) +C NEXT LINE FOR RAH BUG + START(MOTIF) = MAX(START(MOTIF),1) + IEND(MOTIF) = START(MOTIF) + RANGEL(MOTIF) - 1 + END IF + GO TO 10 + END IF +C + END IF +C +C IF WE GET HERE WE HAVE FOUND A NON-ORRED CLASS AND IF THE MOTIF WE CAME +C IN WITH WAS NOT NOTTED +C WE HAVE RESET ITS RANGE AND +C ALL THOSE UP THE LIST THAT ARE ORRED, AND THE CURRENT ONE +C NOW WE MUST FIND THE TOP OF THE NEXT LIST SIGNIFIED BY AN ANDED +C CLASS +C +20 CONTINUE + MOTIF = MOTIF - 1 + IF(COMBIN(MOTIF).NE.'A')GO TO 20 +C IF(COMBIN(MOTIF).EQ.'O')GO TO 20 + ICLASS = CLASS(MOTIF) + ILEN = LENGTH(MOTIF) + CUT = CUTOFF(MOTIF) + IWT = WTSTR(MOTIF) + ISTRST = STRNGS(MOTIF) + COMB = COMBIN(MOTIF) + END +C********************************************************************* + SUBROUTINE MOTIF1(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND, + +MATCHP,MATCHS,IFOUND,CUT,JOB) + CHARACTER SEQ(IDIM1),STRING(IDIM2) + REAL MATCHS + IFOUND = 0 + ISTEP = 1 + IF(JOB.EQ.1)ISTEP = CUT + IF(ISTART.LT.1)ISTART=1 + L1 = IEND-ISTART+1 + IF(ISTART.GT.IDIM1)RETURN + IF(L1.LT.IDIM2)RETURN + CALL FIND8(SEQ(ISTART),L1,STRING,IDIM2,ISTEP,IFOUND) + IF(IFOUND.EQ.0)RETURN +C SAVE MATCH POSITION + MATCHP = ISTART+IFOUND-1 + MATCHS = IDIM2 + END +C********************************************************************* + SUBROUTINE MOTIF2(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND,CUTOFF, + +MATCHP,MATCHS,IFOUND) + CHARACTER SEQ(IDIM1),STRING(IDIM2) + REAL MATCHS + IFOUND = 0 + IF(ISTART.LT.1)ISTART=1 + IF(ISTART.GT.IDIM1)RETURN + CALL SQFIT4(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND,CUTOFF,MATCHS, + +IFOUND) + IF(IFOUND.EQ.0)RETURN +C SAVE MATCH POSITION + MATCHP = IFOUND + END +C********************************************************************* + SUBROUTINE SQFIT4(SEQ,IDIM1,STRING,IDIM2, + 1IS,IE,MINSC,MATCHS,IFOUND) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM1),STRING(IDIM2) + REAL MATCHS,MINSC + INTEGER CTONUM + EXTERNAL CTONUM + MINSCR = MINSC +C + IDIF=(IE-IS+2)-IDIM2 +C IDIF IS THE NUMBER OF POSNS TO TRY +C IPSTR GOES FROM 1 TO IDIM2 IDIF TIMES +C TRY ALL POSSIBLE POSITIONS FOR MATCHING AND SCORE FOR EACH +C POINT TO ARRAY ELEMENT CORRESPONDING TO FIRST BASE + IPSEQ=IS + DO 200 I=1,IDIF + NTOT=0 + IP=IPSEQ + DO 100 J=1,IDIM2 + IF(CTONUM(SEQ(IP)).EQ.CTONUM(STRING(J)))THEN + NTOT = NTOT + 1 + ELSE + IF(STRING(J).EQ.'-')NTOT = NTOT + 1 + END IF + IP=IP+1 +100 CONTINUE +C END OF COUNTING FOR THIS POSITION.IS TOTAL HIGH ENOUGH? + IF(NTOT.GE.MINSCR)THEN + MATCHS = NTOT + IFOUND = IP-IDIM2 + RETURN + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + IFOUND = 0 + END + SUBROUTINE EMBOUT(KEYNAM,FROM,TO,STRAND,DESCRP,IDEV) + CHARACTER KEYNAM*(*),STRAND,DESCRP*(*) + INTEGER FROM,TO +C note keynam*8, strand*1 descrp*38 + WRITE(IDEV,1000,ERR=100)KEYNAM,FROM,TO,STRAND,DESCRP +1000 FORMAT('FT',' ',A8,' ',I6,' ',I6,' ',A,' ',A) + RETURN +100 CONTINUE + END diff --git a/src/staden/pip.f b/src/staden/pip.f new file mode 100644 index 0000000..8fd4b0b --- /dev/null +++ b/src/staden/pip.f @@ -0,0 +1,469 @@ +C PIP (protein interpretation program) +C +C author: Rodger Staden, Medical Research Council Centre, +C Laboratory of Molecular Biology, Hills Road, +C Cambridge, England +C 16-3-90 Removed escape after bpause +C 5-4-90 Changed getdev to redir and all that implies (see redir) +C 27-6-90 added hopp and woods hydrophilicity table. Not used yet! +C but could be sent to compc +C 6-7-90 added showfi +C 9-7-90 Renamed menu routine +C 5-11-90 Very many changes for addition of file of file names +C search for patterns - affects patternp,patternpc,pipl, +C pip,nip,nipl,patternn,patternnc,analps89 and subs89 (rdwmt) +C 4-12-90 CHANGED NAME OF ROUTINE COMPC TO COMPCP because the sun +C linker cannot cope with subroutines and common blocks +C having the same name +C 11-12-90 Changed handling of sequence libraries. New logical name +C set here and passed to rdseq +C 13-04-91 removed initial CLEARV +C The maximum sequence length is defined by the parameter MAXSEQ +C and MAXWIN. +C +C Either Staden (ie. no heading and effectively free format with +C lines of any length <81 and allowing gaps that will be removed) +C or EMBL or GENBANK format files can be read. +C +C 7-6-91 New sequence library routines for cdrom format. PIR stuff junked +C 3-7-91 set namlen = 60 +C 18-7-91 added titles to pattern files. Fixed graphics output for patterns +C 25-2-92 changed call to rdseq + SUBROUTINE FMAIN() + INTEGER BOTOPT,TOPOPT + PARAMETER (NAMLEN = 60) + CHARACTER*(NAMLEN) HELPF,POINTF,FILMAR,FILNAM,FILEP,FILEA,FILEAA + CHARACTER*(NAMLEN) FILEIN,FILE12,FOFNAM + CHARACTER*(NAMLEN) LIBLF + PARAMETER (BOTOPT=0,TOPOPT=28, + + MAXSEQ=100000, + + MXSPAN=603, + + MAXWIN=MAXSEQ+MXSPAN, + + MAXSD2=MAXSEQ/2, + + MAXSD3=MAXSEQ/3, + + MAXWIR=100000, + + MAXD36=MAXWIR/36, + + MAXD2=MAXWIR/2, + + MAXD3=MAXWIR/3, + + MAXMEN=-5, + + MAXOPT=28, + + MAXDEV=9, + + MXWTLN = 120, + + IDM = 26) + PARAMETER ( + + HELPF='PIPHELP', + + POINTF='PIPHPNT', + + FILMAR='PIPMARG', + + FILEA='PROTGRP', + + FILEAA='PROTALL', + + FILEP='PROTMAT', + + FILE12='ROBSON', + + LIBLF='SEQUENCELIBRARIES') + PARAMETER ( MAXMOT = 50,MAXWTS = 4000) + INTEGER HELPS(BOTOPT:TOPOPT),HELPE(BOTOPT:TOPOPT),DEVNOS(MAXDEV) + INTEGER WORKI(MAXWIR),OPT,MARGB(MAXOPT),MARGT(MAXOPT) + INTEGER MATRIX(IDM,IDM),MAT1(IDM,IDM) + REAL WORKR(MAXWIR) + CHARACTER*(NAMLEN) NAMSAV(MAXMOT) + CHARACTER*8 KEYNS(MAXMOT) + CHARACTER SEQ(MAXWIN),SEQW(MAXSEQ),CHRSET(IDM) + REAL HYDRO(IDM),ISOP(IDM),HYDRE(IDM),HYDHW(IDM) + CHARACTER*3 CODONS(26) + EQUIVALENCE (WORKI,WORKR) +C 1,2,3,4,5,6,7,8,9,0,1,2,3,4,5,6,7,8,9,0,1,2,3,4,5,6 +C c,s,t,p,a,g,n,d,e,q,?,?,h,r,k,m,i,l,v,f,y,w + DATA HYDHW/-1.0,0.3,-0.4,0.0,-0.5,0.0,0.2,3.0,3.0,0.2,0.0,0.0, + +-0.5,3.0,3.0,-1.3,-1.8,-1.8,-1.5,-2.5,-2.3,-3.4,0.0,0.0,0.0,0.0/ + DATA HYDRO/70.,36.,38.,29.,63.,41.,10.,10.,10.,10.,10.,10., + +13.,0.,6.,64.,90.,82.,87.,72.,32.,36.,41.,41.,41.,41./ + DATA HYDRE/0.29,-0.18,-0.05,0.12,0.62,0.48,-0.78,-0.9,-0.74, + +-0.85,-0.74,-0.85,-0.4,-2.53,-1.5,0.64,1.38,1.06,1.08,1.19, + +0.26,0.81,0.0,0.0,0.0,0.0/ +C DATA ISOP/5.02,6.68,6.53,6.10,6.02,5.97,5.41,2.87,3.22, +C +5.65,5.00,5.00,7.58,10.76,9.74,5.75,6.02,5.98,5.97,5.98, +C +5.65,5.88,5.00,5.00,5.00,5.00/ + DATA ISOP/0.0,0.0,0.0,0.0,0.0,0.0,0.0,-1.0,-1.0, + +0.0,0.0,0.0,0.0,1.0,1.0,0.0,0.0,0.0,0.0,0.0, + +0.0,0.0,0.0,0.0,0.0,0.0/ + DATA CODONS/'TGY','WSN','ACN','CCN', + +'GCN','GGN','AAY','GAY','GAR','CAR', + +'RAY','SAR','CAY','MGN','AAR','ATG', + +'ATH','YTN','GTN','TTY','TAY','TGG', + +'---','---','---','---'/ +C Initialise help + CALL INTHLP('pip', TOPOPT) +C GET DEVICE NUMBERS + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + CALL OPENGR(DEVNOS(3)) + CALL GIDMAT(MAT1,IDM,22) + IGORT = 0 + IFORNO = 0 + LIBNO = 2 + WRITE(KBOUT,1000) +1000 FORMAT( + +' PIP (Protein interpretation program) V5.0 Feb 1992',/, + +' Author: Rodger Staden'/) +C READ IN THE POINTERS TO THE HELP FILE + CALL SETHLP(HELPS,HELPE,BOTOPT,TOPOPT,POINTF,DEVNOS(4),KBOUT) + CALL INITGR(KBIN,KBOUT,HELPS(0),HELPE(0),HELPF,DEVNOS(4)) + CALL INITLU(IDM) + IOK=0 +C IF(MACTYP.EQ.MACSHT)CALL OPENB(DEVNOS(5),FILBUF,LREC,NREC,IOK) +C IF(IOK.NE.0)STOP +C GET SCREEN AND MARGIN SIZES + CALL GETMRG(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + +MAXOPT,DEVNOS(1),FILMAR) + IIIIX=0 + IIIIY=0 + IDEV=KBOUT + MOPT=0 + IDIMT = 0 +C READ IN THE SCORE MATRIX (USUALLY MDM78) + CALL GETMAT(DEVNOS(1),FILEP,MATRIX,IDM,CHRSET,KBOUT,IOK) + IF(IOK.NE.0) GO TO 9999 + CALL SETPAR(IOK) + IF(IOK.NE.0) GO TO 9999 +2 CONTINUE + IDEVLL = DEVNOS(5) + IDEVEN = DEVNOS(6) + IDEVAN = DEVNOS(7) + IDEVDL = DEVNOS(8) + IDEVLF = DEVNOS(9) + CALL RDSEQ( + +SEQ(2+MXSPAN/2),MAXSEQ,IDIMT,J1,J2,ISTART,IEND,IDIM1,IDIMB, + +DEVNOS(1),FILNAM,KBIN,KBOUT, + +HELPS(3),HELPE(3),HELPF,DEVNOS(4),IDEV,IFORNO, + +IDEVLL,IDEVEN,IDEVAN,IDEVDL, + +IDEVLF,LIBNO,LIBLF,WORKI,MAXWIR,IOK) + IF(IOK.NE.0)GO TO 1 + FILEIN=FILNAM + CALL SHOWFI(KBOUT,FILEIN) +C GIVE COMPOSITION AS A CHECK + IF(IDIMB.GT.0)CALL MWCALC(SEQ(2+MXSPAN/2),IDIMB, + +J1-ISTART+1,J2-ISTART+1,KBOUT,KBOUT) +C set pointers to sequence +******** +C MAXSEQ = THE DIMENSION OF THE RAM BUFFER SEQ +C IDIMT = THE ACTUAL SEQUENCE LENGTH (AND THEREFORE THE NUMBER OF ELEMENTS +C IN THE DISK BUFFER) +C ISTART = THE SEQUENCE NUMBER OF THE CHARACTER OCCUPYING SEQ(1) +C J1 = THE SEQUENCE NUMBER OF THE FIRST CHARACTER IN THE ACTIVE REGION +C J2 = THE SEQUENCE NUMBER OF THE LAST CHARACTER IN THE ACTIVE REGION +C IDIM1 = J2-J1+1 I.E. THE NUMBER OF ELEMENTS IN THE ACTIVE REGION +C IEND = THE SEQUENCE NUMBER OF THE LAST ELEMENT OF SEQ +C IDIMB = IEND-ISTART+1 I.E. THE NUMBER OF ELEMENTS IN THE RAM BUFFER +******** + IDIM1P=IDIMB+MXSPAN +1 CONTINUE + CALL BPAUSE(KBIN,KBOUT,IOK) +C IF(IOK.NE.0) GO TO 9999 +C give menu, get option +C + CALL MENU(OPT,KOPT,MOPT,MAXOPT,MAXMEN,KBIN,KBOUT, + + HELPS(0),HELPE(0),HELPF,DEVNOS(4)) +C change region + IF((OPT.EQ.4).AND.(IDIMT.GT.0))THEN +C CALL REDEFA +C + (SEQ(2+MXSPAN/2),IDIMT,J1,J2,MAXSEQ,IDIM1,ISTART,IEND,IDIMB, +C + DEVNOS(5),KBIN,KBOUT,IOK,SEQW,LREC,NREC,HELPS(OPT), +C + HELPE(OPT),HELPF,DEVNOS(4),MACTYP,MACSHT,MACLNG) + CALL GTREG(KBIN,KBOUT,ISTART,IEND,J1,J2, + + 'Define active region', + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),IOK) + IDIM1 = J2 - J1 + 1 + GO TO 1 + END IF +C STOP + IF(OPT.EQ.2)GO TO 9999 +C +C LIST +C + IF((OPT.EQ.5).AND.(IDIMB.GT.0))THEN + CALL LSTSEP(SEQ(2+MXSPAN/2),IDIMB,IDEV,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C WRITE OUT ACTIVE SEQUENCE +C + IF((OPT.EQ.8).AND.(IDIM1.GT.0))THEN + CALL WRTACT(DEVNOS(1),FILNAM,KBIN,KBOUT, + + SEQ(J1+1-ISTART+1+MXSPAN/2),IDIM1, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C ruler +C + IF((OPT.EQ.12).AND.(IDIM1.GT.0))THEN + CALL RULER(J1,J2,MARGL,MARGR, + + MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,KBIN,KBOUT,1, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C composition +C + IF((OPT.EQ.22).AND.(IDIM1.GT.0))THEN + CALL COMPH(SEQ,IDIM1P,MXSPAN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1,J2,KBIN,KBOUT,HYDRO,IDM, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C clear all +C + IF(OPT.EQ.10)THEN + CALL CLEARG + GO TO 1 + END IF +C +C xhairs +C + IF((OPT.EQ.13).AND.(IDIM1.GT.0))THEN + XMAX=J2 + XMIN=J1 + YMAX=ISYMAX + YMIN=0. + CALL CLEARV + CALL XHAIRN(XMAX,XMIN,YMAX,YMIN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + ISXMAX,ISYMAX,IIIIX,IIIIY,N,KBOUT, + + SEQ(2+MXSPAN/2),ISTART,IDIMB, + + SEQ(2+MXSPAN/2),ISTART,IDIMB,1) + GO TO 1 + END IF +C +C clear vt100 +C + IF(OPT.EQ.11)THEN + CALL CLEARV + GO TO 1 + END IF +C +C CHANGE MARGINS +C + IF(OPT.EQ.14)THEN + CALL MARGC(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + + HELPS(OPT),HELPE(OPT),MAXOPT,HELPF,DEVNOS(4),KBIN,KBOUT) + GO TO 1 + END IF +C +C plot map +C + IF((OPT.EQ.16).AND.(IDIM1.GT.0))THEN + CALL PLTMAP(DEVNOS(1),FILNAM,IDIM1,MARGL,MARGR,MARGB(OPT), + + MARGT(OPT),ISXMAX,ISYMAX,J1,J2,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C new file +C + IF(OPT.EQ.3)GO TO 2 +C +C type text +C + IF(OPT.EQ.6)THEN + CALL TTEXT(DEVNOS(1),FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C help +C + IF(OPT.EQ.1)THEN + CALL HELP(HELPS,HELPE,BOTOPT,TOPOPT,HELPF,DEVNOS(4), + + KBIN,KBOUT) + GO TO 1 + END IF +C +C search +C + IF((OPT.EQ.17).AND.(IDIM1.GT.0))THEN + KOPT = 1 + CALL SERCHP(SEQ(2+MXSPAN/2),IDIMB,J1,J2,ISTART,SEQW,MAXSD3, + + SEQW(1+MAXSD3),MAXSD3, + + SEQW(1+2*MAXSD3),MAXSD3, + + WORKI,WORKI(1+10*MAXD36),WORKI(1+20*MAXD36),10*MAXD36, + + WORKI(1+30*MAXD36),WORKI(1+31*MAXD36),WORKI(1+32*MAXD36), + + WORKI(1+33*MAXD36), + + WORKI(1+34*MAXD36),WORKI(1+35*MAXD36),MAXD36, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + IDEV,DEVNOS(1),FILNAM,FILEA,FILEAA,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C direct output to disk +C + IF(OPT.EQ.7)THEN + CALL REDIR(IDEV,DEVNOS(2),DEVNOS(3),IGORT,FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C signal searches from named plot files +C + IF((OPT.EQ.20).AND.(IDIM1.GT.0))THEN + CALL SIGNLP(SEQ(2+MXSPAN/2),IDIMB, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + ISXMAX,ISYMAX,J1-ISTART+1,J2-ISTART+1, + + DEVNOS(1),FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),IDEV, + + WORKI,WORKR(1+IDM*MXWTLN),WORKI(1+2*IDM*MXWTLN),IDM, + + MXWTLN,SEQW,CHRSET,KOPT) + GO TO 1 + END IF +C +C SEQFIT +C + IF((OPT.EQ.18).AND.(IDIM1.GT.0))THEN + CALL SQPFIT(SEQ(2+MXSPAN/2),IDIMB,SEQW,MAXSEQ, + + WORKI,WORKI(1+MAXD2),MAXD2, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1,J2,ISTART,IDEV, + + DEVNOS(1),FILNAM,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C calc mol wt +C + IF((OPT.EQ.21).AND.(IDIM1.GT.0))THEN + CALL MWCALC(SEQ(2+MXSPAN/2),IDIMB, + + J1-ISTART+1,J2-ISTART+1,KBOUT,IDEV) + GO TO 1 + END IF +C +C plot charge +C + IF((OPT.EQ.23).AND.(IDIM1.GT.0))THEN + CALL COMPCP(SEQ,IDIM1P,MXSPAN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + J1-ISTART+1,J2-ISTART+1,KBIN,KBOUT,ISOP,IDM, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C sepfit +C + IF((OPT.EQ.19).AND.(IDIM1.GT.0))THEN + CALL SEPFIT(SEQ(2+MXSPAN/2),IDIMB,SEQW,MAXSEQ, + + WORKI,WORKI(1+MAXD2),MAXD2, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,J1,J2, + + ISTART,IDEV, + + DEVNOS(1),FILNAM,KBIN,KBOUT,MATRIX,IDM, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C write labels +C + IF(OPT.EQ.15)THEN + CALL LABLER(KBIN,KBOUT,ISXMAX,ISYMAX, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4)) + GO TO 1 + END IF +C +C show settings and edit +C + IF(OPT.EQ.9)THEN + WRITE(KBOUT,4000)FILEIN,J1,J2,IDIM1 +4000 FORMAT(' Sequence=',A,/,' Start=',I7,' End=',I7,' Length=',I7) +C THIS NEXT LINE IS BECAUSE SOMETIMES THE FILE IS NOT READY TO EDIT! + CLOSE(UNIT=DEVNOS(2)) + CALL SEQEDT(SEQ(2+MXSPAN/2),MAXSEQ,IDIMB,KBIN, + + KBOUT,HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),DEVNOS(1), + + FILNAM,FILEIN,IDM,IOK) + IF(IOK.NE.0) GO TO 1 +C CALL EDITSQ(SEQ(2+MXSPAN/2),MAXSEQ,SEQW,MAXSEQ,IDIMB,KBIN, +C + KBOUT,HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),1,DEVNOS(1), +C + FILNAM,PAA,IDM) +******** +C MAXSEQ = THE DIMENSION OF THE RAM BUFFER SEQ +C IDIMT = THE ACTUAL SEQUENCE LENGTH (AND THEREFORE THE NUMBER OF ELEMENTS +C IN THE DISK BUFFER) +C ISTART = THE SEQUENCE NUMBER OF THE CHARACTER OCCUPYING SEQ(1) +C J1 = THE SEQUENCE NUMBER OF THE FIRST CHARACTER IN THE ACTIVE REGION +C J2 = THE SEQUENCE NUMBER OF THE LAST CHARACTER IN THE ACTIVE REGION +C IDIM1 = J2-J1+1 I.E. THE NUMBER OF ELEMENTS IN THE ACTIVE REGION +C IEND = THE SEQUENCE NUMBER OF THE LAST ELEMENT OF SEQ +C IDIMB = IEND-ISTART+1 I.E. THE NUMBER OF ELEMENTS IN THE RAM BUFFER +C THIS IS A FUDGE AND ONLY WORKS FOR UNBUFFERED SEQUENCES!!!!!!!!!!! + J1 = 1 + J2 = IDIMB + IDIMT = IDIMB + IEND = IDIMB + IDIM1 = IDIMB + WRITE(KBOUT,4000)FILEIN,J1,J2,IDIM1 + GO TO 1 + END IF +C +C robson +C + IF((OPT.EQ.24).AND.(IDIM1.GT.0))THEN + CALL ROBSON(SEQ(2+MXSPAN/2),IDIMB, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,J1-ISTART+1, + + J2-ISTART+1,KBIN,KBOUT, + + DEVNOS(1),FILE12, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),IDEV,KOPT) + GO TO 1 + END IF +C +C hydrophobic moment +C + IF((OPT.EQ.25).AND.(IDIM1.GT.0))THEN + CALL HYDMOM(SEQ(J1+1-ISTART+1+MXSPAN/2),IDIM1,HYDRE,IDM, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT), + + ISXMAX,ISYMAX,KBIN,KBOUT, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C helix wheel +C + IF((OPT.EQ.26).AND.(IDIM1.GT.0))THEN + CALL HELIXW(SEQ(2+MXSPAN/2),IDIMB,ISTART,J1,J2, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,KBIN,KBOUT, + + HYDRE,IDM, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C back translate +C + IF((OPT.EQ.27).AND.(IDIM1.GT.0))THEN + CALL BACK(SEQ(J1+1-ISTART+1+MXSPAN/2),IDIM1, + + CODONS,KBIN,KBOUT,FILNAM, + + DEVNOS(1),SEQW,MAXSEQ,WORKR,IDIM1P,MXSPAN, + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),KOPT) + GO TO 1 + END IF +C +C pattern search +C + IF((OPT.EQ.28).AND.(IDIM1.GT.0))THEN + CALL PATTEN(SEQ(J1-ISTART+2+MXSPAN/2),IDIM1,SEQW,MAXSEQ, + + WORKI(1),WORKI(MAXMOT+1),WORKI(2*MAXMOT+1),WORKI(3*MAXMOT+1), + + WORKI(4*MAXMOT+1),WORKI(5*MAXMOT+1),WORKI(6*MAXMOT+1), + + WORKI(7*MAXMOT+1),WORKI(8*MAXMOT+1),WORKI(9*MAXMOT+1), + + WORKI(10*MAXMOT+1),WORKI(11*MAXMOT+1),WORKI(12*MAXMOT+1), + + WORKI(13*MAXMOT+1),WORKI(14*MAXMOT+1),WORKI(15*MAXMOT+1), + + WORKI(16*MAXMOT+1),WORKI(17*MAXMOT+1),WORKI(18*MAXMOT+1), + + WORKI(19*MAXMOT+1),WORKI(20*MAXMOT+1), + + WORKR(22*MAXMOT+1),WORKR(23*MAXMOT+1), + + FILNAM,MAXMOT,MAXWTS,MATRIX,IDEV,DEVNOS(6),DEVNOS(7), + + MARGL,MARGR,MARGB(OPT),MARGT(OPT),ISXMAX,ISYMAX,J1, + + KBIN,KBOUT,DEVNOS(8),IDM,SEQ(1),MAT1,NAMSAV,KEYNS,CHRSET, + + HELPS(OPT),HELPE(OPT),HELPF,DEVNOS(4),FOFNAM,DEVNOS(1)) +C NB IVE SENT THE START OF SEQ TO BE USED IN PATTEN. THIS IS OK +C AS LONG AS MAXMOT0. (IF IT IS NOT THE CURRENT MOTIF IS A NOT THAT HAS +C ALREADY BEEN SEARCHED FOR +C + IFOUND = 0 + IF(START(MOTIF).GT.0)THEN +C +C + IF(ICLASS.EQ.1)THEN + CALL MOTIF1(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF),IFOUND, + + CUTOFF(MOTIF),0) + ELSE IF(ICLASS.EQ.2)THEN + CALL MOTIF2(SEQ,IDSEQ,STRING(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),CUT,MATCHP(MOTIF),MATCHS(MOTIF),IFOUND) + ELSE IF(ICLASS.EQ.3)THEN + CALL MOTFI3(SEQI,IDSEQ,STRNGI(ISTRST),ILEN,START(MOTIF), + + IEND(MOTIF),CUT,MATCHP(MOTIF),MATCHS(MOTIF),IFOUND,MATRIX,IDM) + ELSE IF(ICLASS.EQ.4)THEN + CALL MOTFI4(SEQI,IDSEQ,ILEN,START(MOTIF), + + IEND(MOTIF),WEIGHT(IWT),CUT,MATCHP(MOTIF),MATCHS(MOTIF), + + IFOUND,IDM) + ELSE IF(ICLASS.EQ.5)THEN + CALL MOTIF8(SEQI,IDSEQ,MATRIX,LENGTH(MOTIF),START(MOTIF), + + IEND(MOTIF),RANGET(MOTIF),RANGEM(MOTIF), + + CUTOFF(MOTIF),MATCHP(MOTIF),MATCHS(MOTIF), + + IENTRY(MOTIF),IFOUND,MATCHQ(MOTIF), + + LAST5(MOTIF),LAST3S(MOTIF),LAST3E(MOTIF),IDM) + ELSE IF(ICLASS.EQ.6)THEN + CALL MOTFI4(SEQI,IDSEQ,ILEN,START(MOTIF), + + IEND(MOTIF),WEIGHT(IWT),CUT,MATCHP(MOTIF),MATCHS(MOTIF), + + IFOUND,IDM) + ELSE + WRITE(KBOUT,*)'UNKNOWN CLASS!!' + END IF +C +C + END IF +C +C +C MATCH FOUND WHEN MATCH WANTED ? +C +C +C + IF(((IFOUND.EQ.0).AND.(COMB.NE.'N')).OR. + + ((IFOUND.GT.0).AND.(COMB.EQ.'N')))THEN +C +C NO SO GO BACK OR SIDEWAYS ONE MOTIF +C +C + CALL BAKSID(CLASS,LENGTH,CUTOFF,STRNGS,NMOT, + + MOTIF,ICLASS,ILEN,CUT,IWT,ISTRST,WTSTR, + + RELMOT,START,IEND,MATCHQ,RANGES,RANGEL,RELEND,IRET,MATCHP, + + COMBIN,COMB) +C +C +C IF CANT GO BACK ANY FURTHER QUIT + IF(IRET.NE.0) THEN + IF(MAXSS.GT.MINMSS) THEN + CALL DSPLAZ(MATCHP,LENGTH,NMOT,SEQ,IDSEQ,IDEVOT, + + CLASS,MATCHQ,IOPT, + + KSTART,MATCHS,CUTSCR,MINSCR,MAXSCR, + + TITLE,FILNAM, + + MATRIX,MAT1,IDM,PMINT,PMAXT,PROBT, + + WEIGHT,MAXWTS,WTSTR,CUTOFF,PMINC,RANGES,RANGEL,IDEVN, + + JSTART,KEYNS,BESTP,BESTQ,BESTS,2,MAXSS) + ITOTAL = ITOTAL + 1 + END IF + RETURN + END IF +C +C + ELSE +C +C +C MATCH FOUND. +C +C +C PREPARE FOR NEXT SEARCH THIS MOTIF BY INCREMENTING POINTER +C TO SEARCH RANGE (NOT FOR CLASS 6 WHICH IS HANDLED BY MOTIF6) +C +C + IF(COMB.EQ.'N')THEN + START(MOTIF) = -9 + ELSE + IF(ICLASS.NE.5) START(MOTIF) = MATCHP(MOTIF) + 1 + END IF +C +C TRY GOING FORWARD ONE MOTIF +C +C + CALL FORWAD(CLASS,LENGTH,CUTOFF,NMOT, + + MOTIF,ICLASS,ILEN,CUT,IWT,RELMOT,START,IEND, + + RANGES,RANGEL,STRNGS,ISTRST,WTSTR,IDSEQ,IENTRY, + + RANGET,RANGEM,START2,IEND2,MATCHQ,RELEND,MATCHP,IDSPLY, + + COMBIN,COMB) +C +C +C +C IS THIS THE LAST MOTIF? IF SO DISPLAY THE MATCH +C +C + IF(IDSPLY.EQ.1)THEN + IF(JOPT.NE.1) ITOTAL = ITOTAL + 1 + CALL DSPLAZ(MATCHP,LENGTH,NMOT,SEQ,IDSEQ,IDEVOT, + + CLASS,MATCHQ,IOPT, + + KSTART,MATCHS,CUTSCR,MINSCR,MAXSCR, + + TITLE,FILNAM, + + MATRIX,MAT1,IDM,PMINT,PMAXT,PROBT, + + WEIGHT,MAXWTS,WTSTR,CUTOFF,PMINC,RANGES,RANGEL,IDEVN, + + JSTART,KEYNS,BESTP,BESTQ,BESTS,JOPT,MAXSS) +C +C +C HORRIBLE SPECIAL CASE - IF LAST MOTIF IS NOTTED WE MUST MOVE BACK AFTER +C DISPLAY + IF(COMB.EQ.'N')THEN + CALL BAKSID(CLASS,LENGTH,CUTOFF,STRNGS,NMOT, + + MOTIF,ICLASS,ILEN,CUT,IWT,ISTRST,WTSTR, + + RELMOT,START,IEND,MATCHQ,RANGES,RANGEL,RELEND,IRET,MATCHP, + + COMBIN,COMB) +C +C +C IF CANT GO BACK ANY FURTHER QUIT + IF(IRET.NE.0) THEN + IF(MAXSS.GT.MINMSS) THEN + CALL DSPLAZ(MATCHP,LENGTH,NMOT,SEQ,IDSEQ,IDEVOT, + + CLASS,MATCHQ,IOPT, + + KSTART,MATCHS,CUTSCR,MINSCR,MAXSCR, + + TITLE,FILNAM, + + MATRIX,MAT1,IDM,PMINT,PMAXT,PROBT, + + WEIGHT,MAXWTS,WTSTR,CUTOFF,PMINC,RANGES,RANGEL,IDEVN, + + JSTART,KEYNS,BESTP,BESTQ,BESTS,2,MAXSS) + ITOTAL = ITOTAL + 1 + END IF + RETURN + END IF + END IF + END IF +C +C + END IF +C +C +C GO BACK FOR NEXT SEARCH +C +C + GO TO 100 + END +C********************************************************************* + SUBROUTINE MOTFI3(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND,CUTOFF, + +MATCHP,MATCHS,IFOUND,MATRIC,IDM) + INTEGER SEQ(IDIM1),STRING(IDIM2) + INTEGER MATRIC(IDM,IDM) + REAL MATCHS + IFOUND = 0 + IF(ISTART.LT.1)ISTART=1 + IF(ISTART.GT.IDIM1)RETURN + CALL SQFTI5(SEQ,IDIM1,STRING,IDIM2,ISTART,IEND,CUTOFF,MATCHS, + +IFOUND,MATRIC,IDM) + IF(IFOUND.EQ.0)RETURN +C SAVE MATCH POSITION + MATCHP = IFOUND + RETURN + END +C********************************************************************* + SUBROUTINE SQFTI5(SEQ,IDIM1,STRING,IDIM2, + 1IS,IE,MINSC,MATCHS,IFOUND,MATRIC,IDM) +C AUTHOR: RODGER STADEN + INTEGER SEQ(IDIM1),STRING(IDIM2) + REAL MATCHS,MINSC + INTEGER MATRIC(IDM,IDM) + MINSCR = MINSC +C + IDIF=(IE-IS+2)-IDIM2 +C IDIF IS THE NUMBER OF POSNS TO TRY +C IPSTR GOES FROM 1 TO IDIM2 IDIF TIMES +C TRY ALL POSSIBLE POSITIONS FOR MATCHING AND SCORE FOR EACH +C POINT TO ARRAY ELEMENT CORRESPONDING TO FIRST BASE + IPSEQ=IS + DO 200 I=1,IDIF + NTOT=0 + IP=IPSEQ + DO 100 J=1,IDIM2 + NTOT = NTOT + MATRIC(SEQ(IP),STRING(J)) + IP=IP+1 +100 CONTINUE +C END OF COUNTING FOR THIS POSITION.IS TOTAL HIGH ENOUGH? + IF(NTOT.GE.MINSCR)THEN + MATCHS = NTOT + IFOUND = IP-IDIM2 + RETURN + END IF + IPSEQ=IPSEQ+1 +200 CONTINUE + IFOUND = 0 + RETURN + END +C********************************************************************* + SUBROUTINE MOTFI4(SEQ,IDIM1,LENGTH,ISTART,IEND, + +WEIGHT,CUTOFF,MATCHP,MATCHS,IFOUND,IDM) + REAL WEIGHT(IDM,LENGTH) + REAL MATCHS + INTEGER SEQ(IDIM1) + IFOUND = 0 + IF(ISTART.LT.1)ISTART=1 + L1 = IEND-ISTART+1 + IF(ISTART.GT.IDIM1)RETURN + IF(L1.LT.LENGTH)RETURN + DO 10 I=ISTART,ISTART+L1-LENGTH + SUM = 0. + K = 0 + DO 5 J=I,I+LENGTH-1 + K = K + 1 + SUM = SUM + WEIGHT(SEQ(J),K) + 5 CONTINUE + IF(SUM.GE.CUTOFF) THEN + MATCHP = I + MATCHS = SUM + IFOUND = I + RETURN + END IF + 10 CONTINUE + IFOUND = 0 + END +C********************************************************************* + SUBROUTINE FMOTI4(SEQ,IDIM,WT,LENGTH,CUTOFF,SUM,IFOUND,IDM) +C AUTHOR: RODGER STADEN + INTEGER SEQ(IDIM) + REAL WT(IDM,LENGTH) + DO 10 I=1,IDIM-LENGTH+1 + SUM=0. + K=0 + DO 5 J=I,I+LENGTH-1 + K=K+1 + SUM=SUM+WT(SEQ(J),K) +5 CONTINUE + IF(SUM.GE.CUTOFF)THEN + IFOUND = I + RETURN + END IF +10 CONTINUE + IFOUND = 0 + END +C********************************************************************* + SUBROUTINE MOTIF8(SEQ,IDSEQ,MATRIX,LENGTH,I5STAR,I5END, + + I3STAR,I3END,CUTOFF,MATCHP,MATCHS, + + IENTRY,IFOUND,MATCHQ, + + LAST5,LAST3S,LAST3E,IDM) +C AUTHOR: RODGER STADEN + INTEGER SEQ(IDSEQ) + INTEGER MATRIX(IDM,IDM),REPEET + REAL MATCHS + EXTERNAL REPEET +C WE HAVE A START POSITION FOR THE 5' END OF THE 5' END OF +C A POTENTIAL REPEAT I5STAR AND AN END DEFINED BY A RANGE I5END +C WE HAVE A REPEAT LENGTH LENGTH +C WE HAVE A RANGE OF POSITIONS FOR THE 3' STEM TO START +C I3STAR TO I3END +C TRY THE TIGHTEST LOOPS FIRST +C BUT FIRST WE MAY HAVE TO FINISH A PREVIOUS SEARCH +C THIS IS DENOTED BY IENTRY NE 0. +C NOTE IENTRY IS ALSO USED TO RETURN THE 3' MATCH POSITION + ICUT = CUTOFF +C WRITE(*,*)'ICUT',ICUT + IFOUND = 0 + JENTRY = IENTRY + IENTRY = 0 + IF(I5STAR.LT.1)I5STAR=1 + IF((I5STAR+I3STAR+LENGTH-2).GT.IDSEQ)RETURN + IF(JENTRY.NE.0)THEN + I1 = LAST5 +C WRITE(*,*)'I1,LAST3S,LAST3E',I1,LAST3S,LAST3E + DO 50 J=LAST3S+1,LAST3E + J1 = J + ISUM = REPEET(SEQ,IDSEQ,MATRIX,LENGTH,I1,J1,IDM) +C RETURN IF GOOD ENOUGH + IF(ISUM.GE.ICUT)THEN + MATCHP = I1 + IENTRY = J1 + MATCHQ = J1 + MATCHS = ISUM + IFOUND = MATCHP + LAST3S = J1 + RETURN + END IF +50 CONTINUE +C NOW MOVE 5' STEM START POSITION (WE HAVE JUST FINISHED THE LAST) +C TO THE LAST MATCH + 1 + I5STAR = MATCHP + 1 + END IF +C SET ENTRY FLAG TO ZERO TO SIGNIFY LAST SEARCH NOW COMPLETED + IENTRY = 0 + ISUM = 0 + LOOPI1 = I5STAR + IF((I5STAR+I3STAR+LENGTH-2).GT.IDSEQ)RETURN + LOOPI2 = MIN(IDSEQ-2*LENGTH+1,I5END) +C WRITE(*,*)'IDSEQ,LENGTH,I5STAR,I5END', +C +IDSEQ,LENGTH,I5STAR,I5END +C +C TRY ALL STEM STARTS FROM 5' START TO 5' END +C +C + DO 200 I = LOOPI1,LOOPI2 +C +C + I1 = I +C +C TRY ALL LOOPS FROM 3' START TO 3' END +C + LOOPJ1 = I + I3STAR -1 + IF((LOOPJ1+LENGTH-1).GT.IDSEQ)RETURN + LOOPJ2 = MIN(IDSEQ-LENGTH+1,I+I3END-1) + +C WRITE(*,*)'I3STAR,I3END',I3STAR,I3END +C +C +C + DO 100 J = LOOPJ1,LOOPJ2 +C +C +C + J1 = J +C IN REPEAT NOTE THAT +C THE 5' END POINTER I1 GOES FORWARDS +C THE 3' END POINTER J1 GOES FORWARDS +C + ISUM = REPEET(SEQ,IDSEQ,MATRIX,LENGTH,I1,J1,IDM) +C RETURN IF GOOD ENOUGH +C WRITE(*,*)ISUM + IF(ISUM.GE.ICUT)THEN + MATCHP = I1 + IENTRY = J1 + MATCHQ = J1 + MATCHS = ISUM + IFOUND = MATCHP +C SAVE CURRENT POSITION FOR LATER ENTRIES + LAST5 = I1 + LAST3S = J1 + LAST3E = LOOPJ2 + RETURN + END IF +100 CONTINUE +200 CONTINUE + END +C********************************************************************* + INTEGER FUNCTION REPEET(SEQ,IDSEQ,MATRIX,LENGTH,I5P,I3P,IDM) + INTEGER SEQ(IDSEQ) + INTEGER MATRIX(IDM,IDM) +C THE 5' END POINTER GOES FORWARDS +C THE 3' END POINTER GOES FORWARDS + L=0 + I5=I5P-1 + I3=I3P-1 + DO 100 I=1,LENGTH + I5 = I5 + 1 + I3 = I3 + 1 +C WRITE(*,*)'I5,I3',I5,I3 + L5 = SEQ(I5) + L3 = SEQ(I3) + L = L + MATRIX(L5,L3) +100 CONTINUE + REPEET = L + END + SUBROUTINE DSPLAZ(MATCHP,LENGTH,NMOT,SEQ,IDSEQ,IDEV, + +CLASS,MATCHQ,IOPT,KSTART,MATCHS,CUTSCR,MINSCR,MAXSCR, + +TITLE,FILNAM, + + MATRIX,MAT1,IDM,PMINT,PMAXT,PROBT, + + WEIGHT,MAXWTS,WTSTR,CUTOFF,PMINC,RANGES,RANGEL, + + IDEV1,JSTART,KEYNS,BESTP,BESTQ,BESTS,JOPT,MAXSS) + INTEGER MATCHP(NMOT),LENGTH(NMOT),CLASS(NMOT) + INTEGER MATCHQ(NMOT) + CHARACTER SEQ(IDSEQ),TITLE*(*),FILNAM*(*) + REAL MATCHS(NMOT),MINSCR,MAXSCR + INTEGER MATRIX(IDM,IDM),MAT1(IDM,IDM),WTSTR(NMOT) + REAL WEIGHT(MAXWTS),CUTOFF(NMOT) + INTEGER RANGES(NMOT),RANGEL(NMOT) + CHARACTER DASH + CHARACTER*(*) KEYNS(NMOT) +C stuff for best + INTEGER BESTP(NMOT),BESTQ(NMOT) + REAL BESTS(NMOT),MAXSS + EXTERNAL PSCORE + SAVE DASH + DATA DASH/'-'/ +C jopt 1 get best match for any individual sequence, then +C display it. So check each match for being best (>maxss), if it is +C save its coords in bestp, bestq. Keep a note that a score +C has been recorded for this sequence (actually noted by bestp(1) ne.0) +C when we finish a sequence (denoted by jopt = 2) put all the +C saved values into matchp, matchq and process as normal. + IF(JOPT.NE.0) THEN + IF(JOPT.EQ.2) THEN + DO 5 I=1,NMOT + MATCHP(I) = BESTP(I) + MATCHQ(I) = BESTQ(I) + MATCHS(I) = BESTS(I) + 5 CONTINUE + ELSE IF(JOPT.EQ.1) THEN +C Add scores + T = 0. + DO 6 I = 1,NMOT + IF(MATCHP(I).NE.0) T = T + MATCHS(I) + 6 CONTINUE + IF(T.GT.MAXSS) THEN + DO 7 I=1,NMOT + BESTP(I) = MATCHP(I) + BESTQ(I) = MATCHQ(I) + BESTS(I) = MATCHS(I) + 7 CONTINUE + MAXSS = T + END IF + RETURN + END IF + END IF +C Add scores + T = 0. + DO 10 I = 1,NMOT + IF(MATCHP(I).NE.0) T = T + MATCHS(I) +10 CONTINUE + POBS = 1.0 + IF(PMINC.LT.1.0)THEN +C Calc prob + DO 20 I = 1,NMOT + IF(MATCHP(I).NE.0)THEN + CALL GETP(CLASS(I),SEQ(MATCHP(I)+KSTART-1),LENGTH(I), + + IDM,MATRIX,MAT1,WEIGHT(MAX(1,WTSTR(I)))) + PROB = PSCORE(MATCHS(I)) + POBS = POBS * PROB + END IF +20 CONTINUE + IF((PMINC.LT.1.0).AND.(POBS.GT.PMINC))RETURN + END IF +C + IF(T.GT.MAXSCR) MAXSCR = T + IF(T.LT.MINSCR) MINSCR = T + IF(T.LT.CUTSCR) RETURN +C +C Motif by motif +C + IF(IOPT.EQ.1)THEN + WRITE(IDEV,1001)FILNAM(1:10),T,TITLE +1001 FORMAT(' >',A,' ',F10.3,' ',A) + DO 100 I=1,NMOT + J = I +C +C Check for no match (needed for ored motifs) +C + IF(MATCHP(J).NE.0)THEN + WRITE(IDEV,1000)MATCHP(J)+KSTART-1,MATCHS(J),KEYNS(I) + WRITE(IDEV,1002) + + (SEQ(K),K=MATCHP(J),MATCHP(J)+LENGTH(J)-1) +C Repeat ? + IF(CLASS(J).EQ.5)THEN + WRITE(IDEV,1002) + + (SEQ(K),K=MATCHQ(J),MATCHQ(J)+LENGTH(J)-1) + WRITE(IDEV,1000)MATCHQ(J)+KSTART-1 + END IF + END IF +100 CONTINUE +1000 FORMAT(' ',I7,' ',F10.3,' ',A8) +1002 FORMAT(' ',60A1) + IF(PMINC.LT.1.0)WRITE(IDEV,1004)POBS +1004 FORMAT(' Probability =',E10.4) + RETURN + END IF +C +C Title,score only +C + IF(IOPT.EQ.3)THEN + WRITE(IDEV,1001)FILNAM(1:10),T,TITLE + IF(PMINC.LT.1.0)WRITE(IDEV,1004)POBS +1003 FORMAT(' ',F12.5) + END IF +C +C Inclusive +C + IF(IOPT.EQ.2)THEN + WRITE(IDEV,1001)FILNAM(1:10),T,TITLE + MINP = 999999 + MAXP = -999999 + DO 300 I = 1, NMOT + K = MATCHP(I) + IF(K.NE.0)THEN + IF(K.LT.MINP)MINP = K + K = K + LENGTH(I) - 1 +C Repeat ? + IF(CLASS(I).EQ.5) K = MATCHQ(I) + LENGTH(I) - 1 + IF(K.GT.MAXP)MAXP = K + END IF +300 CONTINUE + WRITE(IDEV,1000)MINP+KSTART-1 + WRITE(IDEV,1002) + + ((SEQ(K1),K1=K2,MIN(K2+59,MAXP)),K2=MINP,MAXP,60) + IF(PMINC.LT.1.0)WRITE(IDEV,1004)POBS + RETURN + END IF +C +C write file of whole seq +C + IF(IOPT.EQ.4)THEN + WRITE(IDEV,1001)FILNAM(1:10),T,TITLE + CALL OPENRS(IDEV1,FILNAM,IOK,LLL,1) + IF(IOK.NE.0)THEN + WRITE(IDEV,*)' Error opening sequence file' + RETURN + END IF +C Want first character from sequence to start at jstart +C So how many dashes required? + IDASH = JSTART - MATCHP(1) + IF(IDASH.GT.0)CALL PADOUT(IDEV1,DASH,60,IDASH) +C Write up to and including first motif + J1 = MATCHP(1)+LENGTH(1)-1 + WRITE(IDEV1,1005,ERR=401)(SEQ(K),K=1,J1) + DO 400 I=2,NMOT + J = I +C Put dashes for next gap in now. what is max gap? + IMAXG = RANGES(J) - LENGTH(J-1) + RANGEL(J) - LENGTH(J) +C What is actual gap? + IG = MATCHP(J) - MATCHP(J-1) - LENGTH(J-1) +C Want to put in difference number of dashes + IDASH = IMAXG - IG + IF(IDASH.GT.0)CALL PADOUT(IDEV1,DASH,60,IDASH) +C Write up to and including next motif + J2 = MATCHP(J) + LENGTH(J) - 1 + WRITE(IDEV1,1005,ERR=401)(SEQ(K),K=J1+1,J2) + J1 = J2 +400 CONTINUE +C Write to end of sequence + WRITE(IDEV1,1005,ERR=401)(SEQ(K),K=J1+1,IDSEQ) +401 CONTINUE + CLOSE(UNIT=IDEV1) +1005 FORMAT(' ',60A1) + END IF +C +C write file inclusive +C + IF(IOPT.EQ.5)THEN + WRITE(IDEV,1001)FILNAM(1:10),T,TITLE + CALL OPENRS(IDEV1,FILNAM,IOK,LLL,1) + IF(IOK.NE.0)THEN + WRITE(IDEV,*)' Error opening sequence file' + RETURN + END IF +C Write first motif + J1 = MATCHP(1)+LENGTH(1)-1 + WRITE(IDEV1,1005,ERR=501)(SEQ(K),K=MATCHP(1),J1) + DO 500 I=2,NMOT + J = I +C Put dashes for next gap in now. what is max gap? + IMAXG = RANGES(J) - LENGTH(J-1) + RANGEL(J) - LENGTH(J) +C What is actual gap? + IG = MATCHP(J) - MATCHP(J-1) - LENGTH(J-1) +C Want to put in difference number of dashes + IDASH = IMAXG - IG + IF(IDASH.GT.0)CALL PADOUT(IDEV1,DASH,60,IDASH) +C Write up to and including next motif + J2 = MATCHP(J) + LENGTH(J) - 1 + WRITE(IDEV1,1005,ERR=501)(SEQ(K),K=J1+1,J2) + J1 = J2 +500 CONTINUE +501 CONTINUE + CLOSE(UNIT=IDEV1) + END IF + END + SUBROUTINE PADOUT(IDEV,CHAR,LINLEN,NCHAR) + CHARACTER CHAR +C HOW MANY LINES? + NLINE = 1 + (NCHAR-1)/LINLEN + K1 = 1 + K2 = MIN(NCHAR,LINLEN) + DO 10 I = 1,NLINE + WRITE(IDEV,1000,ERR=20)(CHAR,K=K1,K2) + K1 = K2 + 1 + K2 = K1 + LINLEN - 1 + K2 = MIN(K2,NCHAR) +10 CONTINUE +20 CONTINUE +1000 FORMAT(' ',60A1) + END + SUBROUTINE SETCMP(COMPIN,IDM) + PARAMETER (MAXCHR = 26) + REAL COMPIN(IDM) + COMMON /COMPC/COMP(MAXCHR) + SAVE /COMPC/ + DO 10 I = 1,MAXCHR + COMP(I) = 0.0 +10 CONTINUE + T = 0. + DO 20 I = 1,IDM + COMP(I) = COMPIN(I) + T = T + COMPIN(I) +20 CONTINUE + DO 30 I = 1,IDM + COMP(I) = COMP(I) / T +30 CONTINUE + END diff --git a/src/staden/pipmen.f b/src/staden/pipmen.f new file mode 100644 index 0000000..06b7fec --- /dev/null +++ b/src/staden/pipmen.f @@ -0,0 +1,88 @@ + SUBROUTINE MENU(OPT,KOPT,MOPT,MAXOPT,MINMEN,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + INTEGER OPT + INTEGER IHELPS(0:MAXOPT),IHELPE(0:MAXOPT) + CHARACTER HELPF*(*) +1 CONTINUE + CALL CLEARV + IF(MOPT.EQ.0)THEN + WRITE(KBOUT,5000) +5000 FORMAT(' Menus and their numbers are '/ + +' m0 = This menu'/ + 1' m1 = General'/ + 2' m2 = Screen control'/ + 5' m3 = Statistical analysis of content'/ + 1' m4 = Structure'/ + 3' m5 = Search'/ + 6' ? = Help'/ + 1' ! = Quit') + ELSE IF(MOPT.EQ.-1)THEN + WRITE(KBOUT,5001) +5001 FORMAT(' General menu'/ + + ' 0 = List of menus'/ + 1 ' 3 = Read new sequence'/ + 1 ' 4 = Redefine active region'/ + + ' 5 = List the sequence'/ + + ' 6 = List a text file'/ + + ' 7 = Direct output to disk'/ + + ' 8 = Write active sequence to disk'/ + + ' 9 = Edit the sequence'/ + + ' 17 = Short sequence search'/ + + ' 18 = Compare a sequence'/ + + ' 19 = Compare a sequence using a score matrix'/ + + ' 27 = Back translate to dna') + ELSE IF(MOPT.EQ.-2)THEN + WRITE(KBOUT,5002) +5002 FORMAT(' Screen control menu'/ + + ' 0 = List of menus'/ + + ' 10 = Clear graphics'/ + + ' 11 = Clear text'/ + + ' 12 = Draw a ruler'/ + + ' 13 = Use cross hair'/ + + ' 14 = Reset margins'/ + + ' 15 = Label diagram'/ + + ' 16 = Display a map') + ELSE IF(MOPT.EQ.-3)THEN + WRITE(KBOUT,5005) +5005 FORMAT(' Statistical analysis of content'/ + + ' 0 = List of menus'/ + + ' 21 = Count amino acid composition'/ + + ' 22 = Plot hydrophobicity'/ + + ' 23 = Plot charge',/ + + ' 25 = Plot hydrophobic moment') + ELSE IF(MOPT.EQ.-4)THEN + WRITE(KBOUT,5007) +5007 FORMAT(' Structure menu'/ + + ' 0 = List of menus'/ + + ' 22 = Plot hydrophobicity'/ + + ' 23 = Plot charge'/, + + ' 24 = Plot robson prediction',/ + + ' 25 = Plot hydrophobic moment',/ + + ' 26 = Draw helix wheel') + ELSE IF(MOPT.EQ.-5)THEN + WRITE(KBOUT,5004) +5004 FORMAT(' Search menu'/ + + ' 0 = List of menus'/ + 1 ' 17 = Search for short sequences'/ + 2 ' 18 = Compare a sequence'/ + 2 ' 19 = Compare a sequence using a score matrix'/ + 2 ' 20 = Search for a sequence using a weight matrix'/ + 2 ' 28 = Search for patterns of motifs') + END IF + WRITE(KBOUT,1001) +1001 FORMAT(' ? Menu or option number=',$) + CALL GETOPT(KBIN,KOPT,OPT) + IF((OPT.LT.MINMEN).OR.(OPT.GT.MAXOPT).OR.(KOPT.GT.2)) THEN + MOPT = 0 + GO TO 1 + END IF + IF((KOPT.EQ.2).AND.(OPT.GE.0)) THEN + CALL HELP2(IHELPS(OPT),IHELPE(OPT),HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(OPT.LE.0) THEN + MOPT = OPT + GO TO 1 + END IF + END diff --git a/src/staden/pl4010.f b/src/staden/pl4010.f new file mode 100644 index 0000000..ac1a4cc --- /dev/null +++ b/src/staden/pl4010.f @@ -0,0 +1,554 @@ +C PL4010 +C AUTHOR: RODGER STADEN +C 5-4-90 Changed to allow redirection of graphics to a file +C and included ndc which was in plot89 +C 15-10-91 Moved IANDRS, IORRS and WRITEB to seeme.f +C SET OF FORTRAN77 GRAPHICS ROUTINES FOR TEKTRONIX 4010 +C AS IMPLEMENTED ON THE VT640 AND CIFER T4 +C OPENGR OPENS GRAPHICS OUTPUT ON DEVICE IDEVGR +C IANDRS PERFORMS LOGICAL .AND. ON INTEGERS +C IORRS PERFORMS LOGICAL .OR. ON INTEGERS +C WRITEI CONVERTS INTEGER VARIABLES TO CHARACTER STRINGS +C WRITEB WRITES OUT CHARACTERS STORED IN A CHARACTER STRING +C WRITEB IS THE ONLY ROUTINE THAT WRITES TO THE GRAPHICS DEVICE +C AND NEEDS TO SUPPRESS CARRIAGE RETURN, LINE FEED. +C THIS IS DONE ON THE VAX BY SENDING A NUL CHARACTER, +C OR ON THE SUN USING PUTC, OR ON THE IBM USING TRANSPARENT +C I/O. NOTE ON SOME MACHINES E.G.THE SUN BYTE SWITCHING MAY BE +C REQUIRED. +C XY4010 RETURNS THE CODES FOR 4010 X,Y COORDS +C ALPHAM PUTS THE TERMINAL IN ALPHA MODE +C VECTOM PUTS THE TERMINAL IN VECTOR MODE +C VT100M PUTS THE TERMINAL IN VT100 OR TRANSPARENT MODE +C BUFFGR RECEIVES AN INTEGER VALUE AND BUFFERS IT, IF +C THE BUFFER IS FULL OR A FLAG (FLSH) IS SET +C THE BUFFER IF FLUSHED +C CLEARG CLEARS THE GRAPHICS SCREEN (BOTH ON VT640) +C MOVEG MOVES TO X,Y +C DRAWG DRAWS TO X,Y +C POINTG DRAWS POINT AT X,Y USING MOVEG AND DRAWG +C XHAIR PUTS UP X HAIR AND RETURNS X,Y +C DELAY DELAYS PROCESS BY DOING NOTHING (REQUIRED FOR MODE SWITCHING) +C CLEARV CLEARS VT100 SCREEN WITHOUT BLANKING GRAPHICS +C WRITEG WRITES TEXT SENT AT GIVEN POSITION IX,IY +C INITGR INITIALISES MODE SWITCHING, WHICH IS DIFFERENT ON DIFFERENT +C MACHINES. SO FAR ONLY CATERS FOR VERSATERM PRO BEING DIFFERENT +C FROM ALL OTHERS WHEN GOING INTO VT100 MODE. ALSO DOES NOT CLEAR +C TEXT PLANE WHEN REQUESTED TO DO SO FOR VERSATERM. +C FLUSHG FLUSHES GRAPHICS BUFFER +C IN TEKTRONIX 4010 THERE ARE 4 MODES: +C ALPHA FOR POSITIONING TEXT +C GRAPHIC PLOT FOR PLOTTING +C GRAPHIC INPUT FOR READING CURSOR POSITIONS +* THESE ROUTINES ARE FOR THE VT640 TERMINAL AND THE CIFER T4 +* BOTH OF WHICH HAVE TWO PLANES: ONE A TEKTRONIX PLANE, THE OTHER +* A SCROLLING TEXT PLANE THAT CAN BE TREATED AS A VT100. +* THEY BOTH OFFER ENHANCEMENTS ON THE 4010 INCLUDING EXTRA MODES +* BUT THEY RESTRICT CHANGES BETWEEN MODES. SO FAR I HAVE ALWAYS +* GONE THROUGH WHAT IVE CALLED VECTOR MODE (EQUIVALENT TO GRAPHIC +* PLOT MODE) +* THE T4 NEEDS TO HAVE A HIGHER LEVEL OF MODES SETTING: +* TO RESPOND TO TEKTRONIX COMMANDS IT REQUIRES A PREVIOUS ESC P t THAT +* HAS NOT BEEN FOLLOWED BY ESC \ WHICH IS THE COMMAND TO GET OUT OF +* THIS HIGHER LEVEL MODE OR STATUS. THIS REQUIRES ALL ENTRIES TO VECTOR +* MODE TO BE PRECEDED BY ESC P T AND ENTRY TO VT100 MODE TO BE CHANGED. +* THESE EXTRA COMMANDS ARE INCLUDED IN THE CODE BUT ARE COMMENTED OUT +* IF YOU HAVE CIFER T4 TERMINALS REMOVE THE C'S FROM THE APPROPRIATE +* LINES IN VT100M AND VECTOM +* STANDARD COMMANDS: +* TEKTRONIX VT640 T4 +* +* INTO GRAPHIC MODE GS GS GS +* (MUST BE IN +* TEKTRONIX MODE) +* INTO ALPHA MODE US +* PUT UP XHAIR ESC SUB ESC SUB +* (MUST BE IN VECTOR MODE) +* ERASE SCREEN, +* ENTER ALPHA MODE, +* HOME ESC FF ESC FF +* (MUST BE IN VECTOR MODE) +* HARD COPY ESC ETB ESC ETB +* (MUST BE IN ALPHA +* MODE) +* ENTER VT100 MODE CAN ESC \ +* (THIS TAKES US +* RIGHT OUT OF +* TEKTRONIX MODE) +* CLEAR VT100 ESC [ 2 J ESC [ 2 J +* + SUBROUTINE XHAIR(IX,IY,TERM) +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 + INTEGER ESC,SUB,FLSH,CHARS(5) + CHARACTER TERM + EXTERNAL IANDRS + SAVE ESC,SUB,FLSH,NO + DATA ESC/27/,SUB/26/,FLSH/1/,NO/0/ +C MUST START IN VECTOR MODES + IF(IGORF.NE.0) RETURN + IF(MODE.NE.VECTOR)CALL VECTOM + CALL BUFFGR(ESC,NO) + CALL BUFFGR(SUB,FLSH) +C GET COORDS FROM TERMINAL + CALL XHM(CHARS,TERM,IDEVGR) +C DECODE THEM + IX=IANDRS(CHARS(2),31) + IX=IX*32 + IX=IX+IANDRS(CHARS(3),31) + IY=IANDRS(CHARS(4),31) + IY=IY*32 + IY=IY+IANDRS(CHARS(5),31) +C +C convert to drawing board units +C + IX = NINT(REAL(IX)/DBTDUX) + IY = NINT(REAL(IY)/DBTDUY) +C THIS EXITS TO ALPHA MODE + MODE=ALPHA + END + SUBROUTINE XY4010(IX,IY,LSX,MSX,LSY,MSY) +C AUTHOR: RODGER STADEN + EXTERNAL IANDRS,IORRS +C ENCODES IX AND IY INTO +C THEIR LEAST AND MOST SIGNIFICANT PARTS FOR 4010 CODES +C MSX=((IX.AND.992)/32).OR.32 +C LSX=(IX.AND.31).OR.64 +C MSY=((IY.AND.992)/32).OR.32 +C LSY=(IY.AND.31).OR.96 + MSX=IANDRS(IX,992) + MSX=MSX/32 + MSX=IORRS(MSX,32) + LSX=IANDRS(IX,31) + LSX=IORRS(LSX,64) + MSY=IANDRS(IY,992) + MSY=MSY/32 + MSY=IORRS(MSY,32) + LSY=IANDRS(IY,31) + LSY=IORRS(LSY,96) + END + SUBROUTINE OPENGR(IDEVG) +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 + CHARACTER*40 FILNAM +C SET DEVICE NAME + IDEVGR=IDEVG +C SET BUFFER POINTER TO ZERO + NBUFF=0 +C SET MAXIMUM BUFFER SIZE + MAXBUF=80 +C SET ARBITRARY VALUES TO MODES + VT100=0 + VECTOR=1 + ALPHA=2 + MODE=0 + CLOSE(UNIT=IDEVGR) + CALL OPENRS(IDEVG,FILNAM,IOK,LRECL,9) + IF(IOK.NE.0)WRITE(*,*)' ERROR OPENING GRAPHICS DEVICE' +C INITIALISE LAST VALUES + LMSY=0 + LLSY=0 + LMSX=0 + LLSX=0 + IGORF = 0 + RETURN + END + SUBROUTINE OPENGF(IDEVG) +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 + IDEVGR = IDEVG + IGORF = 1 + END + SUBROUTINE ALPHAM +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 +C PUTS TERMINAL IN ALPHA MODE + INTEGER US,NO + SAVE US,NO + DATA US/31/,NO/0/ + IF(MODE.EQ.ALPHA)RETURN + CALL DELAY(100000) + IF((ITERM.EQ.2).AND.(MODE.EQ.VT100)) THEN +C SEND ESC [ ? 3 8 h + CALL BUFFGR(27,NO) + CALL BUFFGR(91,NO) + CALL BUFFGR(63,NO) + CALL BUFFGR(51,NO) + CALL BUFFGR(56,NO) + CALL BUFFGR(104,NO) + END IF +C SEND TO OUTPUT ROUTINE + CALL BUFFGR(US,NO) +C SET MODE TO ALHPA + MODE=ALPHA + END + SUBROUTINE VECTOM +C PUTS TERMINAL IN VECTOR MODE +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 + INTEGER GS,NO,UPPERP,LOWERT,ESC + SAVE GS,NO,UPPERP,LOWERT,ESC + DATA GS/29/,NO/0/,UPPERP/80/,LOWERT/116/,ESC/27/ + IF(MODE.EQ.VECTOR)RETURN + CALL DELAY(100000) +C SEND TO OUTPUT ROUTINE +C NEXT THREE LINE REQUIRED FOR CIFER T4 +C CALL BUFFGR(ESC,NO) +C CALL BUFFGR(UPPERP,NO) +C CALL BUFFGR(LOWERT,NO) + IF((ITERM.EQ.2).AND.(MODE.EQ.VT100)) THEN +C SEND ESC [ ? 3 8 h + CALL BUFFGR(27,NO) + CALL BUFFGR(91,NO) + CALL BUFFGR(63,NO) + CALL BUFFGR(51,NO) + CALL BUFFGR(56,NO) + CALL BUFFGR(104,NO) + ELSE IF((ITERM.EQ.3).AND.(MODE.EQ.VT100)) THEN +C SEND ESC [ ? 3 8 h + CALL BUFFGR(27,NO) + CALL BUFFGR(91,NO) + CALL BUFFGR(63,NO) + CALL BUFFGR(51,NO) + CALL BUFFGR(56,NO) + CALL BUFFGR(104,NO) + END IF + CALL BUFFGR(GS,NO) +C SET MODE TO VECTOR + MODE=VECTOR + END + SUBROUTINE VT100M +C PUTS TERMINAL IN VT100 MODE +C NOTE FOR CIFER T4 SEND ESC\ +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 + INTEGER ESC,FLSH,BSLASH,NO + SAVE ESC,FLSH,BSLASH,NO + DATA ESC/27/,FLSH/1/,BSLASH/92/,NO/0/ + IF(MODE.EQ.VT100)RETURN +C CALL DELAY(100000) +C SEND TO OUTPUT ROUTINE +C NEXT TWO LINES RQUIRED FOR CIFER T4 +C CALL BUFFGR(ESC,NO) +C CALL BUFFGR(BSLASH,NO) + IF(ITERM.EQ.0) THEN +C VT640 CAN NUL + CALL BUFFGR(24,NO) + CALL BUFFGR(0,FLSH) + ELSE IF(ITERM.EQ.1) THEN +C VERSATERM ESC 2 + CALL BUFFGR(ESC,NO) + CALL BUFFGR(50,FLSH) + ELSE IF(ITERM.EQ.2) THEN +C THE XTERM BUFFER WILL ONLY BE FLUSHED IF WE CHANGE FROM +C FROM GRAPHICS TO ALPHA MODE + CALL BUFFGR(31,NO) +C XTERM ESC ETX + CALL BUFFGR(ESC,NO) + CALL BUFFGR(3,FLSH) + ELSE IF(ITERM.EQ.3) THEN +C SEND ESC [ ? 3 8 l + CALL BUFFGR(27,NO) + CALL BUFFGR(91,NO) + CALL BUFFGR(63,NO) + CALL BUFFGR(51,NO) + CALL BUFFGR(56,NO) + CALL BUFFGR(108,FLSH) + END IF +C SET MODE TO VT100 + MODE=VT100 + CALL DELAY(100000) + END + SUBROUTINE BLANKG +C CLEARS SCREEN +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 + INTEGER ESC,FF,NO + SAVE ESC,FF,NO + DATA ESC/27/,FF/12/,NO/0/ + CALL DELAY(100000) +C FIRST PUT IN VECTOR MODE + IF(MODE.NE.VECTOR)CALL VECTOM + CALL BUFFGR(ESC,NO) + IF(ITERM.EQ.2) THEN + CALL BUFFGR(12,NO) + ELSE + CALL BUFFGR(FF,NO) + END IF +C SETS MODE TO ALHPA SO PUT TO VT100 + CALL VT100M + END + SUBROUTINE BUFFGR(VALUE,FLSH) +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 + INTEGER VALUE,FLSH,ARRAY(1) +C FLSH=0 MEANS ONLY FLUSH BUFFER IF IT IS FULL +C FLSH=1 MEANS FLUSH BUFFER AFTER ADDING VALUE +C FLSH=2 MEANS FLUSH BUFFER WITHOUT ADDING VALUE + IF(FLSH.EQ.0)THEN + NBUFF=NBUFF+1 +C BUFFER FULL? + IF(NBUFF.GT.MAXBUF)THEN + CALL WRITEI(BUFFER,MAXBUF,IDEVGR) + CALL FLUSHS(IDEVGR) +C RESET BUFFER POINTER TO START FOR NEW VALUE + NBUFF=1 + END IF + BUFFER(NBUFF)=VALUE + ELSE IF(FLSH.EQ.1)THEN + NBUFF=NBUFF+1 + IF(NBUFF.GT.MAXBUF)THEN + CALL WRITEI(BUFFER,MAXBUF,IDEVGR) +C NOW WRITE VALUE +C NOTE SOME COMPILERS OBJECT IF A SINGLE VARIABLE IS SENT +C WHEN A DUMMY ARRAY OF DIMENSION 1 IS EXPECTED. SO USE ARRAY(1) + ARRAY(1)=VALUE + CALL WRITEI(ARRAY,1,IDEVGR) + CALL FLUSHS(IDEVGR) + NBUFF=0 + ELSE +C PUT VALUE IN BUFFER + BUFFER(NBUFF)=VALUE + CALL WRITEI(BUFFER,NBUFF,IDEVGR) + CALL FLUSHS(IDEVGR) + NBUFF=0 + END IF + ELSE IF((FLSH.EQ.2).AND.(NBUFF.GT.0)) THEN + CALL WRITEI(BUFFER,NBUFF,IDEVGR) + CALL FLUSHS(IDEVGR) + NBUFF=0 + END IF + END + SUBROUTINE MOVEXY(IX,IY) +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 + INTEGER NO,GS + SAVE NO,GS + DATA NO/0/,GS/29/ +C SEND GS TO OUTPUT ROUTINE TO PUT INTO VECTOR MODE + CALL BUFFGR(GS,NO) +C +C convert from drawing board units +C + IXT = NINT(REAL(IX)*DBTDUX) + IYT = NINT(REAL(IY)*DBTDUY) + CALL XY4010(IXT,IYT,LSX,MSX,LSY,MSY) +C REMEMBER ORDER IMPORTANT + CALL BUFFGR(GS,NO) + CALL BUFFGR(MSY,NO) + CALL BUFFGR(LSY,NO) + CALL BUFFGR(MSX,NO) + CALL BUFFGR(LSX,NO) +C UPDATE LAST VALUES + LMSY=MSY + LLSY=LSY + LMSX=MSX + LLSX=LSX +C LEAVES IN VECTOR MODE + MODE=VECTOR + END + SUBROUTINE DRAWXY(IX,IY) +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 + INTEGER NO + SAVE NO + DATA NO/0/ + IF(MODE.NE.VECTOR)CALL VECTOM +C +C convert from drawing board units +C + IXT = NINT(REAL(IX)*DBTDUX) + IYT = NINT(REAL(IY)*DBTDUY) + CALL XY4010(IXT,IYT,LSX,MSX,LSY,MSY) +C IF BYTES DO NOT CHANGE THEY DONT NEED TO BE SENT +C EXCEPT LSX WHICH ALWAYS REQUIRED + IF(MSY.NE.LMSY)CALL BUFFGR(MSY,NO) + IF(LSY.NE.LLSY)CALL BUFFGR(LSY,NO) + IF(MSX.NE.LMSX)THEN +C MUST SEND LSY IF MSX CHANGED (BUT NOT IF ALREADY SENT) + IF(LSY.EQ.LLSY)CALL BUFFGR(LSY,NO) + CALL BUFFGR(MSX,NO) + END IF +C ALWAYS SEND LSX + CALL BUFFGR(LSX,NO) +C UPDATE LAST VALUES + LMSY=MSY + LLSY=LSY + LMSX=MSX + LLSX=LSX + END + SUBROUTINE CLEARV +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 + INTEGER ESC,LB,TWO,J,FLSH,NO + SAVE ESC,LB,TWO,J,FLSH,NO + DATA ESC/27/,LB/91/,TWO/50/,J/74/,FLSH/1/,NO/0/ +****** THIS IS A VT100 COMMAND ESC[2J + IF(MODE.NE.VT100)CALL VT100M + IF(IGORF.NE.0) RETURN +C DONT CLEAR FOR VERSATERM OR XTERM AS IT ISNT REQUIRED + IF((ITERM.EQ.1).OR.(ITERM.EQ.2)) RETURN + CALL BUFFGR(ESC,NO) + CALL BUFFGR(LB,NO) + CALL BUFFGR(TWO,NO) + CALL BUFFGR(J,FLSH) + END + SUBROUTINE WRITXY(IX,IY,TEXT,NCHAR) +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 + CHARACTER TEXT*(*) +C ROUTINE TO OUTPUT TEXT AT POSITION IX,IY + CALL MOVEXY(IX,IY) + CALL ALPHAM +C FLUSH BUFFER + CALL BUFFGR(IVAL,2) + CALL WRITEB(TEXT,NCHAR,IDEVGR) + END + SUBROUTINE INITGR(KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH) + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 + CHARACTER HELPF*(*) + PARAMETER (MAXPRM = 12) + CHARACTER PROMPT(4)*(MAXPRM) + PARAMETER (DEVX = 1023.0, + + DEVY = 779.0, + + DBUNIT = 10000) + DBTDUX = DEVX/DBUNIT + DBTDUY = DEVY/DBUNIT +C SET TO VT640 + ITERM = 0 + IN = 3 + PROMPT(1) = 'VT640' + PROMPT(2) = 'VersatermPro' + PROMPT(3) = 'Xterm' + PROMPT(4) = 'MS-Kermit' + CALL RADION('Select graphics mode',PROMPT,4,IN, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IN = IN - 1 + ITERM = MAX(0,IN) + END + SUBROUTINE FLUSHG +C AUTHOR: RODGER STADEN + COMMON /GRAPHS/BUFFER,NBUFF,MODE,VECTOR,ALPHA,VT100,IDEVGR, + + LMSY,LLSY,LMSX,LLSX,MAXBUF,IGORF,ITERM, + + DBTDUX,DBTDUY + SAVE /GRAPHS/ + INTEGER BUFFER(80),VECTOR,ALPHA,VT100 +C ROUTINE TO FLUSH GRAPHICS BUFFER +C FLUSH BUFFER + CALL BUFFGR(IVAL,2) + END + SUBROUTINE NDC(ISXMAX,ISYMAX,XNDC,YNDC) +C AUTHOR RODGER STADEN +C CONVERTS FROM DRAWING BOARD SCALES ISXMAX,ISYMAX TO NORMALIZED DEVICE +C COORDINATES DEFINED BY A PARAMETER STATEMENT + PARAMETER (XMAXDV=1023.,YMAXDV=779.) + XNDC=XMAXDV/REAL(ISXMAX) + YNDC=YMAXDV/REAL(ISYMAX) + END +C SUN/UNIX PL4010 MACHINE SPECIFIC + SUBROUTINE XHM(CHARS,TERM,IDEVGR) + INTEGER CHARS(5) + CHARACTER CCHARS*5,TERM,CHAR + INTRINSIC ICHAR,CHAR +10 CONTINUE + READ(IDEVGR,1000,ERR=10,END=10)CCHARS +1000 FORMAT(A) +C IF THE USER HITS CR WITHOUT A PRECEDING CHARACTER WE GET +C A BLANK RECORD --- WE DETECT THIS BY LOOKING FOR SPACE +C CHARACTERS, THOUGH THIS COULD BE A VALID POSITION! THIS +C ALSO DEPENDS ON THE STRIPPING THAT GOES ON. NOTE: +C (A) WE GET SOME GARBAGE ON THE SCREEN (TOUGH LUCK) +C (B) WE HAVE TO STICK THE TERMINATOR CHAR (CR) IN BY HAND + IF(CCHARS.EQ.' ') THEN + READ(IDEVGR,1000,ERR=10,END=10)CCHARS(2:5) + CCHARS(1:1)=CHAR(13) + END IF +C warning following only works for ascii !!!!!!!!! +C WRITE(*,*)'CHARS 1',CCHARS(1:1) + DO 42 I=1,5 + CHARS(I)= ICHAR(CCHARS(I:I)) +C WRITE(*,*)'CHARS1',I,CCHARS(1:1) +42 CONTINUE +C XTERM CAN GET TIED UP IN ALPHA MODE UNLESS SOMETHING IS PRINTED + CALL BUFFGR(10,0) + TERM=CCHARS(1:1) + END + SUBROUTINE WRITEI(BUFFER,IBUFF,IDEVGR) +C AUTHOR: RODGER STADEN + INTEGER BUFFER(IBUFF) + BYTE BA(80) + CHARACTER STRING*80 + EQUIVALENCE (BA,STRING) + DO 10 I=1,IBUFF + BA(I) = BUFFER(I) +10 CONTINUE + CALL WRITEB(STRING,IBUFF,IDEVGR) + RETURN + END + SUBROUTINE DELAY(TIME) +C AUTHOR: RODGER STADEN + INTEGER TIME + DO 1 I=1,TIME +1 CONTINUE + RETURN + END + SUBROUTINE FLUSHS(IDEVGR) + CALL FLUSH(IDEVGR) + END + SUBROUTINE DOTXY(IX,IY) +C AUTHOR: RODGER STADEN + CALL MOVEXY(IX,IY) + CALL DRAWXY(IX,IY) + END diff --git a/src/staden/plX.c b/src/staden/plX.c new file mode 100644 index 0000000..ddf573d --- /dev/null +++ b/src/staden/plX.c @@ -0,0 +1,513 @@ +/* + Title: plX + + File: plX.c + Purpose: A pseudo-device for graphics under X + Last update: Tue May 22 1990 +*/ + + +/* + This module exists to be syntactically compatible with the + pl4010 pseudo-device for Tektronics terminals. However, it + only implements a single graphics plane. + + This ``device'' operates in a space XMINDEV - XMAXDEV and + YMINDEV - YMAXDEV. Values in an external scale can be converted + to an appropriate scale using ndc_x(). + + There is a notional pen which has a ``current position'' in + the graphics space. + + This is implemented using a Graph widget as a window in which + to plot. This gives us back suitable resize and expose callbacks. + The state of the plot is stored, and recalled when replotting + is necessary, from a plotLog. + + Plotting is actually carried out in the window coordinate system + and this can change dynamically: its current size is given by + (currWidth,currHeight) which are static variables updated when + necessary. There are routines to convert between the two coordinate + systems. + + For the XHAIR call, both key and button events are collected, but + they are all turned into key events. +*/ + + + + +#include + +/* ---- Includes ---- */ + +#include "fort.h" /* IMPORT: int_f definition */ +#include "plX.h" +#include "Graph.h" /* IMPORT: graphWidgetClass */ +#include "main.h" /* IMPORT: dispatchEventsUntil */ +#include "plotLog.h" /* IMPORT: all */ +#include "textOutput.h" /* IMPORT: UpdateTextOutput */ + +#include +#include +#include /* IMPORT: XC_crosshair */ + + + +/* ---- Constants ---- */ + +#define DEVXMIN 0 +#define DEVXMAX 10000 +#define DEVWIDTH (DEVXMAX-DEVXMIN) +#define DEVYMIN 0 +#define DEVYMAX 10000 +#define DEVHEIGHT (DEVYMAX-DEVYMIN) + + + + +/* ---- Types ---- */ + +typedef struct _AppResources { + Pixel fg; +} AppResources; + + +/* ---- Static variables ---- */ + +/* Unchanging state */ +static Display *display; +static Widget goWid; +static GC goGC; + +/* Current window size */ +static Dimension currWidth, currHeight; + +/* Current pen position */ +static int penX = 0; +static int penY = 0; + +/* Graphics input cursor */ +static Cursor ginCursor; + +/* Keypress information for GIN mode */ +Boolean keyPressed = False; +char keyVal; +int keyX; +int keyY; + + + + + +/* ---- Internal routines ---- */ + + +static int scaleX(int x) +/* Convert (fixed) device coordinate to (variable) window coordinate */ +{ return((x*currWidth)/DEVWIDTH); +} + + +static int scaleY(int y) +/* Convert (fixed) device coordinate to (variable) window coordinate */ +{ return(currHeight - ((y*currHeight)/DEVHEIGHT)); +} + + +static int invScaleX(int x) +/* Convert (variable) window coordinate to (fixed) device coordinate */ +{ return((x*DEVWIDTH)/currWidth); +} + + +static int invScaleY(int y) +/* Convert (variable) window coordinate to (fixed) device coordinate */ +{ return(DEVHEIGHT - ((y*DEVHEIGHT)/currHeight)); +} + + +static Boolean KeyPressed(void) +{ return(keyPressed); +} + + + + +/* ---- Callback routines ---- */ + + +static void destroyCallback(Widget w, XtPointer client_data, XtPointer call_data) +{ (void) DestroyPL(); + XFreeCursor(display, ginCursor); +} + + +static void exposeCallback(Widget w, XtPointer client_data, XtPointer call_data) +{ PLItem i; + PLResult res; + char textBuf[256]; + + + /* Replot, using logged commands */ + if (ResetReadPL() != OK) + { fprintf(stderr, "\n*** Trouble with plot temporary file. Exiting.\n"); + finalx(1); + } + i.u.t.textp = textBuf; + while ((res = ReadPL(&i)) == OK) + { switch (i.op) + { case DrawLineOp: + XDrawLine(display, XtWindow(goWid), goGC, + scaleX(i.x1), scaleY(i.y1), + scaleX(i.u.p2.x2), scaleY(i.u.p2.y2)); + break; + + case DrawPointOp: + XDrawPoint(display, XtWindow(goWid), goGC, + scaleX(i.x1), scaleY(i.y1)); + break; + + case DrawStringOp: + XDrawString(display, XtWindow(goWid), goGC, + scaleX(i.x1), scaleY(i.y1), + i.u.t.textp, (int)i.u.t.textl); + break; + }/*endswitch*/; + i.u.t.textp = textBuf; + }/*endwhile*/; + + if (res != EndOfPL) + { fprintf(stderr, "\n*** Trouble with plot temporary file. Exiting.\n"); + finalx(1); + } +} + + +static void inputCallback(Widget w, XtPointer client_data, XtPointer call_data) +{ XEvent *eventP = (XEvent *) call_data; + char buffer[1]; + KeySym key; + int charCount; + XComposeStatus compose; + + keyPressed = True; + + /* Get the key value and the pointer location */ + switch (eventP->type) + { case KeyPress: + charCount = XLookupString((XKeyEvent *)eventP, buffer, 1, + &key, &compose); + keyVal = buffer[0]; + keyX = eventP->xkey.x; + keyY = eventP->xkey.y; + break; + case ButtonPress: + /* We turn a button event into a key event sending an 'S' */ + keyVal = 'S'; + keyX = eventP->xbutton.x; + keyY = eventP->xbutton.y; + break; + } +} + +static Arg arglist_1[] = { + {XtNwidth, (XtArgVal) &currWidth}, + {XtNheight, (XtArgVal) &currHeight}, +}; + + +static void resizeCallback(Widget w, XtPointer client_data, XtPointer call_data) +{ + XtGetValues(goWid, arglist_1, XtNumber(arglist_1)); + XClearWindow(display, XtWindow(goWid)); +} + + + + +/* ---- Exported routines ---- */ + + +/* ---- Initialisation routines ---- */ + + +static Arg getSizeArglist_2[] = +{ {XtNwidth, (XtArgVal) &currWidth}, + {XtNheight, (XtArgVal) &currHeight}, + }; + +static XtResource resources_2[] = +{ {XtNforeground, XtCForeground, XtRPixel, (Cardinal)sizeof(Pixel), + XtOffset(AppResources *, fg), XtRString, "XtdefaultForeground"}, + }; + + +Widget CreateGraphicsOutput(Widget parentWid) +/* + Create and return a Graph widget as a child of `parentWid'. + Must be called before opengr_ and initgr_. + + This routine initializes the statics `display', `goGC' and `goWid'. +*/ +{ AppResources app_resources; + + static Arg createArglist[2]; + + /* Find the application foreground colour to use. + */ + XtGetApplicationResources(parentWid, (XtPointer) &app_resources, + resources_2, XtNumber(resources_2), NULL, 0); + + + /* Create the graph widget and add its callbacks. + */ + XtSetArg(createArglist[0], XtNgraphColour1, app_resources.fg); + goWid = XtCreateManagedWidget("graph", graphWidgetClass, parentWid, + createArglist, 1); + XtAddCallback(goWid, XtNcallback, inputCallback, NULL); + XtAddCallback(goWid, XtNdestroyCallback, destroyCallback, NULL); + XtAddCallback(goWid, XtNexposeCallback, exposeCallback, NULL); + XtAddCallback(goWid, XtNresizeCallback, resizeCallback, NULL); + + + /* Get some permanent state. + */ + goGC = GraphGC1(goWid); + display = XtDisplay(goWid); + ginCursor = XCreateFontCursor(display, XC_crosshair); + XtGetValues(goWid, getSizeArglist_2, XtNumber(getSizeArglist_2)); + + + if (CreatePL() != OK) + { fprintf(stderr, "\n*** Can't create plot temporary file. Exiting.\n"); + finalx(1); + } + + + return(goWid); +} + + + + +void opengr_x(int_f *IDEVG_p) +/* + Open graphics output. This routine must be called before + initgr_x. The argument is ignored. +*/ +{ +} + + +void initgr_x(int_f *KBIN_p, int_f *KBOUT_p, + int_f *IHELPS_p, int_f *IHELPE_p, char *HELPF_p, + int_f *IDEVH_p, + int_fl HELPF_l) +/* + This routine must be called before any of the subsequent + routines are used. All arguments are ignored. +*/ +{ +} + + +void opengf_x(int_f *IDEVG_p) +/* + This routine does nothing. +*/ +{ +} + + + + +/* ---- Mode routines ---- */ + + +void alpham_x(void) +/* + This function has no effect. +*/ +{ +} + + +void vectom_x(void) +/* + This function has no effect. +*/ +{ +} + + +void vt100m_x(void) +/* + This function has no effect. +*/ +{ +} + + +/* Clearing routines +*/ + + +void clearv_x(void) +/* + This function does nothing. +*/ +{ +} + + +void blankg_x(void) +/* + This function clears the graphics screen. +*/ +{ XClearWindow(display, XtWindow(goWid)); + if (ResetWritePL() != OK) + { fprintf(stderr, "\n*** Trouble with plot temporary file. Exiting.\n"); + finalx(1); + } +} + + + + +/* ---- Moving and plotting routines ---- */ + + +void movexy_x(int_f *IX_p, int_f *IY_p) +/* + Move the graphics pen to the indicated position. +*/ +{ penX = *IX_p; + penY = *IY_p; +} + + +void drawxy_x(int_f *IX_p, int_f *IY_p) +/* + Draw a line from the current position of the graphics + pen to the indicated position. Leave the pen there. +*/ +{ PLItem i; + + XDrawLine(display, XtWindow(goWid), goGC, + scaleX(penX), scaleY(penY), + scaleX(*IX_p), scaleY(*IY_p)); + + i.op = DrawLineOp; + i.x1 = penX; + i.y1 = penY; + i.u.p2.x2 = *IX_p; + i.u.p2.y2 = *IY_p; + if (WritePL(i) != OK) + { fprintf(stderr, "\n*** Trouble with plot temporary file. Exiting.\n"); + finalx(1); + } + + penX = *IX_p; + penY = *IY_p; +} + + +void dotxy_x(int_f *IX_p, int_f *IY_p) +/* + Plot a single point at (IX,IY). Leave the pen there. +*/ +{ PLItem i; + + XDrawPoint(display, XtWindow(goWid), goGC, + scaleX(*IX_p), scaleY(*IY_p)); + + i.op = DrawPointOp; + i.x1 = *IX_p; + i.y1 = *IY_p; + if (WritePL(i) != OK) + { fprintf(stderr, "\n*** Trouble with plot temporary file. Exiting.\n"); + finalx(1); + } + + penX = *IX_p; + penY = *IY_p; +} + + +void writxy_x(int_f *IX_p, int_f *IY_p, + char *TEXT_p, int_f *NCHAR_p, + int_fl TEXT_l) +/* + NCHAR characters from TEXT are written at position (IX,IY). + The pen position is left undefined. +*/ +{ PLItem i; + + XDrawString(display, XtWindow(goWid), goGC, + scaleX(*IX_p), scaleY(*IY_p), TEXT_p, *NCHAR_p); + + i.op = DrawStringOp; + i.x1 = *IX_p; + i.y1 = *IY_p; + i.u.t.textp = TEXT_p; + i.u.t.textl = *NCHAR_p; + if (WritePL(i) != OK) + { fprintf(stderr, "\n*** Trouble with plot temporary file. Exiting.\n"); + finalx(1); + } +} + + +void flushg_x(void) +/* + Try very hard to ensure all graphics are visible. +*/ +{ XFlush(display); +} + + + + +/* ---- Input routines ---- */ + + +void xhair_x(int_f *IX_p, int_f *IY_p, char *TERM_p, int_fl TERM_l) +/* + The cursor in the graphics window is converted to a crosshair + and the function waits for a key or button to be pressed. When + this occurs the function returns with the cursor location (in + device space) and the key hit. Pressing a mouse button is + equivalent to hitting the 'S' key. The location returned is + not defined if the cursor was out of the graphics window. + + The text output widget is refreshed with all output since this + was last called. + X events are cycled until a key event has occurred. The location + of this is converted to device space and returned along with the + terminating character. +*/ +{ Arg oldCursorArg[1]; + Arg newCursorArg[1]; + Cursor oldCursor; + + + /* Get the old cursor */ + XtSetArg(oldCursorArg[0], XtNcursor, &oldCursor); + XtGetValues(goWid, oldCursorArg, 1); + + /* Set the new cursor */ + XtSetArg(newCursorArg[0], XtNcursor, ginCursor); + XtSetValues(goWid, newCursorArg, 1); + + UpdateTextOutput(); + keyPressed = False; + dispatchEventsUntil(KeyPressed); + *IX_p = (int_f) invScaleX(keyX); + *IY_p = (int_f) invScaleY(keyY); + *TERM_p = keyVal; + + /* Reset the old cursor */ + XtSetArg(oldCursorArg[0], XtNcursor, oldCursor); + XtSetValues(goWid, oldCursorArg, 1); +} diff --git a/src/staden/plX.h b/src/staden/plX.h new file mode 100644 index 0000000..b37cf43 --- /dev/null +++ b/src/staden/plX.h @@ -0,0 +1,196 @@ +/* + Title: plX + + File: plX.h + Purpose: A pseudo-device for graphics under X + Last update: Tue May 15 1990 +*/ + + +/* + This module exists to be syntactically compatible with the + pl4010 pseudo-device for Tektronics terminals. However, it + only implements a single graphics plane. + + This ``device'' operates in a space XMINDEV - XMAXDEV and + YMINDEV - YMAXDEV. Values in an external scale can be converted + to an appropriate scale using ndc_x(). + + There is a notional pen which has a ``current position'' in + the graphics space. +*/ + + + + +/* + C (internal) - SUN FORTRAN (external) name conversions + This table is for the compiler specifically mentioned above, so + as well as being hostage to its horrors we can make use of its + advantages, such as long identifiers. +*/ + +#define opengr_x opengr_ +#define initgr_x initgr_ +#define opengf_x opengf_ +#define alpham_x alpham_ +#define vectom_x vectom_ +#define vt100m_x vt100m_ +#define clearv_x clearv_ +#define blankg_x blankg_ +#define movexy_x movexy_ +#define drawxy_x drawxy_ +#define dotxy_x dotxy_ +#define writxy_x writxy_ +#define flushg_x flushg_ +#define xhair_x xhair_ +#define ndc_x ndc_ + + + + +/* ---- Includes ---- */ + +#include "fort.h" +#include /* IMPORT: Widget */ + + + + +/* ---- Initialisation routines ---- */ + + +extern Widget CreateGraphicsOutput(Widget parentWid); +/* + Create and return a Graph widget as a child of `parentWid'. + Must be called before opengr_ and initgr_. +*/ + + +extern void opengr_x(int_f *IDEV_p); +/* + Open graphics output. This routine must be called before + initgr_x. The argument is ignored. +*/ + + +extern void initgr_x(int_f *KBIN_p, int_f *KBOUT_p, + int_f *IHELPS_p, int_f *IHELPE_p, char *HELPF_p, + int_f *IDEVH_p, + int_fl HELPF_l); +/* + This routine must be called before any of the subsequent + routines are used. All arguments are ignored. +*/ + + +extern void opengf_x(int_f *IDEVG_p); +/* + This routine does nothing. +*/ + + + + +/* ---- Mode routines ---- */ + + +extern void alpham_x(void); +/* + This function has no effect. +*/ + + +extern void vectom_x(void); +/* + This function has no effect. +*/ + + +extern void vt100m_x(void); +/* + This function has no effect. +*/ + + +/* Clearing routines. +*/ + +extern void clearv_x(void); +/* + This function has no effect. +*/ + + +extern void clearg_x(void); +/* + This function clears the graphics screen. +*/ + + + + +/* ---- Moving and plotting routines ---- */ + + +extern void movegr_x(int_f *IX_p, int_f *IY_p); +/* + Move the graphics pen to the indicated position. +*/ + + +extern void drawgr_x(int_f *IX_p, int_f *IY_p); +/* + Draw a line from the current position of the graphics + pen to the indicated position. Leave the pen there. +*/ + + +extern void pointg_x(int_f *IX_p, int_f *IY_p); +/* + Plot a single point at (IX,IY). Leave the pen there. +*/ + + +extern void writet_x(int_f *IX_p, int_f *IY_p, + char *TEXT_p, int_f *NCHAR_p, + int_fl TEXT_l); +/* + NCHAR characters from TEXT are written at position (IX,IY). + The pen position is left undefined. +*/ + + +extern void flushg_x(void); +/* + Try very hard to ensure all graphics are visible. +*/ + + + + +/* ---- Input routines ---- */ + + +extern void xhair_x(int_f *IX_p, int_f *IY_p, char *TERM_p, int_fl TERM_l); +/* + The cursor in the graphics window is converted to a crosshair + and the function waits for a key or button to be pressed. When + this occurs the function returns with the cursor location (in + device space) and the key hit. Pressing a mouse button is + equivalent to hitting the 'S' key. The location returned is + not defined if the cursor was out of the graphics window. +*/ + + + + +/* ---- Enquiry routines ---- */ + + +extern void ndc_x(int_f *ISXMAX_p, int_f *ISYMAX_p, /* in: external scale */ + float *XNDC_p, float *YNDC_p); /* out: scaling factor */ +/* + Return a scaling factor between the external and internal scales. +*/ + diff --git a/src/staden/plot92.f b/src/staden/plot92.f new file mode 100644 index 0000000..1b9147d --- /dev/null +++ b/src/staden/plot92.f @@ -0,0 +1,699 @@ +C PLOT89 +C AUTHOR: RODGER STADEN +C +C 13-8-92 new organisation: let pl4010 do device scaling and hardwire +C it for 1023x768 +C +C +C 25-1-90 XHAIRN changed order of arguments to diabox and fmt4ln +C so that horizontal and vertical sequences were consistent +C with rest of sip +C 5-4-90 Removed ndc and put it in pl4010. added redir +C 4-5-90 Added the new routines for sap +C 14-11-90 Replaced radio by radion +C 2-3-92 set filnam = ' ' for calls to openf1 +C ROUTINES IN THIS LIBRARY +C +C LINE +C POINT +C FRAME +C TEXT +C RULER +C SCALES +C BLIP +C XHAIRS +C XHAIRN +C INQGT +C REDIR +C line + SUBROUTINE LINE(XF,XT,YF,YT,XMAX,XMIN,YMAX,YMIN, + +IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) +C AUTHOR: RODGER STADEN +C DRAWS A LINE FROM XF,YF TO XT,YT. COORDINATE SYSTEMS ARE +C 1) WORLD SYSTEM: XMIN TO XMAX, YMIN TO YMAX +C 2) DRAWING BOARD 0 TO ISXMAX, 0 TO ISYMAX +C 3) DEVICE COORDINATES 0 TO MAXDVX, 0 TO MAXDVY +C THE POSITION OF THE PLOT IS DEFINED IN THE DRAWING BOARD SYSTEM BY A +C BOX STARTING AT IX0,IY0 WITH SIDES IXLEN, IYLEN (IN DRAWING BOARD UNITS) +C RANGE IN WORLD COORDS + RANGWX=XMAX-XMIN + IF(RANGWX.EQ.0.0)RETURN + RANGWY=YMAX-YMIN + IF(RANGWY.EQ.0.0)RETURN +C CLIP + CALL CLIP(XF,XMAX,XMIN,YF,YMAX,YMIN,XFF,YFF) + CALL CLIP(XT,XMAX,XMIN,YT,YMAX,YMIN,XTT,YTT) +C PROPORTIONAL X POSITIONS + XWF=(XFF-XMIN)/RANGWX + XWT=(XTT-XMIN)/RANGWX +C PROPORTIONAL Y POSITIONS + YWF=(YFF-YMIN)/RANGWY + YWT=(YTT-YMIN)/RANGWY +C POSITIONS IN DRAWING BOARD UNITS + XDF=IX0+XWF*IXLEN + XDT=IX0+XWT*IXLEN + YDF=IY0+YWF*IYLEN + YDT=IY0+YWT*IYLEN +C POSITIONS IN DEVICE UNITS + IXF=NINT(XDF) + IXT=NINT(XDT) + IYF=NINT(YDF) + IYT=NINT(YDT) +C DRAW IT + CALL MOVEGR(IXF,IYF) + CALL DRAWGR(IXT,IYT) + END +C POINT + SUBROUTINE POINT(XF,YF,XMAX,XMIN,YMAX,YMIN, + +IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) +C AUTHOR: RODGER STADEN +C DRAWS A POINT AT XF,YF. COORDINATE SYSTEMS ARE +C 1) WORLD SYSTEM: XMIN TO XMAX, YMIN TO YMAX +C 2) DRAWING BOARD 0 TO ISXMAX, 0 TO ISYMAX +C 3) DEVICE COORDINATES 0 TO MAXDVX, 0 TO MAXDVY +C THE POSITION OF THE PLOT IS DEFINED IN THE DRAWING BOARD SYSTEM BY A +C BOX STARTING AT IX0,IY0 WITH SIDES IXLEN, IYLEN (IN DRAWING BOARD UNITS) +C RANGE IN WORLD COORDS + RANGWX=XMAX-XMIN + IF(RANGWX.EQ.0.0)RETURN + RANGWY=YMAX-YMIN + IF(RANGWY.EQ.0.0)RETURN +C CLIP + CALL CLIP(XF,XMAX,XMIN,YF,YMAX,YMIN,XFF,YFF) +C PROPORTIONAL X POSITIONS + XWF=(XFF-XMIN)/RANGWX +C PROPORTIONAL Y POSITIONS + YWF=(YFF-YMIN)/RANGWY +C POSITIONS IN DRAWING BOARD UNITS + XDF=IX0+XWF*IXLEN + YDF=IY0+YWF*IYLEN + IXF=NINT(XDF) + IYF=NINT(YDF) +C PLOT IT + CALL POINTG(IXF,IYF) + END + SUBROUTINE FRAME(IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) +C AUTHOR: RODGER STADEN +C DRAWS A BOX FROM IX0,IY0 OF SIDES IXLEN,IYLEN COORDINATE SYSTEMS ARE +C 2) DRAWING BOARD 0 TO ISXMAX, 0 TO ISYMAX +C 3) DEVICE COORDINATES 0 TO MAXDVX, 0 TO MAXDVY + IXF=IX0 + IXT=IX0+IXLEN + IYF=IY0 + IYT=IY0+IYLEN + CALL MOVEGR(IXF,IYF) + CALL DRAWGR(IXF,IYT) + CALL DRAWGR(IXT,IYT) + CALL DRAWGR(IXT,IYF) + CALL DRAWGR(IXF,IYF) + END +C TEXT + SUBROUTINE TEXT(XF,YF,CHARS,NCHARS,ISIZE,XMAX,XMIN,YMAX,YMIN, + +IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX) +C AUTHOR: RODGER STADEN +C CHARACTER CHARS(NCHARS) + CHARACTER CHARS*(*) +C WRITE TEXT AT XF,YF. COORDINATE SYSTEMS ARE +C 1) WORLD SYSTEM: XMIN TO XMAX, YMIN TO YMAX +C 2) DRAWING BOARD 0 TO ISXMAX, 0 TO ISYMAX +C 3) DEVICE COORDINATES 0 TO MAXDVX, 0 TO MAXDVY +C THE POSITION OF THE PLOT IS DEFINED IN THE DRAWING BOARD SYSTEM BY A +C BOX STARTING AT IX0,IY0 WITH SIDES IXLEN, IYLEN (IN DRAWING BOARD UNITS) +C RANGE IN WORLD COORDS + RANGWX=XMAX-XMIN + IF(RANGWX.EQ.0.0)RETURN + RANGWY=YMAX-YMIN + IF(RANGWY.EQ.0.0)RETURN +C CLIP +C IF OFF SCREEN DONT WRITE + IF(XF.GT.XMAX)RETURN + IF(XF.LT.XMIN)RETURN + IF(YF.GT.YMAX)RETURN + IF(YF.LT.YMIN)RETURN +C PROPORTIONAL X POSITIONS + XWF=(XF-XMIN)/RANGWX +C PROPORTIONAL Y POSITIONS + YWF=(YF-YMIN)/RANGWY +C POSITIONS IN DRAWING BOARD UNITS + XDF=IX0+XWF*IXLEN + YDF=IY0+YWF*IYLEN + IXF=NINT(XDF) + IYF=NINT(YDF) +C PLOT IT + CALL WRITET(IXF,IYF,CHARS,NCHARS) + END + SUBROUTINE SCALES(XMAX,XMIN,YMAX,YMIN, + +IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX, + +BIT,IBLIPH,FIRST,ISCALE) +C AUTHOR: RODGER STADEN +C ROUTINE TO PUT A SCALE ALONG AN AXIS EITHER X OR Y +C DEPENDING ON ISCALE (1=X,2=Y AXES) +C BLIPS OF HEIGHT IBLIPH ARE PLACED ALONG THE AXIS EVERY +C BIT OF LENGTH STARTING FROM FIRST. FIRST CAN BE USED +C TO MAKE SURE THAT A BLIP OCCURS AT A PARTICULAR POSITION +C FOR EXAMPLE AT A ZERO POINT, OTHERWISE FIRST IS SET TO +C XMIN OR YMIN +C 1) WORLD SYSTEM: XMIN TO XMAX, YMIN TO YMAX +C 2) DRAWING BOARD 0 TO ISXMAX, 0 TO ISYMAX +C 3) DEVICE COORDINATES 0 TO MAXDVX, 0 TO MAXDVY +C THE POSITION OF THE PLOT IS DEFINED IN THE DRAWING BOARD SYSTEM BY A +C BOX STARTING AT IX0,IY0 WITH SIDES IXLEN, IYLEN (IN DRAWING BOARD UNITS) + IF(ISCALE.EQ.1)THEN +C NUMBER OF POINTS + NP=1+(XMAX-FIRST)/BIT +C RANGE IN WORLD COORDS + RANGWX=XMAX-XMIN + IF(RANGWX.EQ.0.0)RETURN +C GET END POINTS IN Y + IYF=IY0 + IYT=IY0+IBLIPH +C CALC AND PLOT POINTS + DO 10 I=1,NP +C PROPORTIONAL X POSITIONS + XFF=FIRST+(I-1)*BIT + XWF=(XFF-XMIN)/RANGWX +C POSITION IN DRAWING BOARD UNITS + XDF=IX0+XWF*IXLEN + IXF=NINT(XDF) + CALL MOVEGR(IXF,IYF) + CALL DRAWGR(IXF,IYT) +10 CONTINUE + END IF + IF(ISCALE.EQ.2)THEN +C NUMBER OF POINTS + NP=1+(YMAX-FIRST)/BIT +C RANGE IN WORLD COORDS + RANGWY=YMAX-YMIN + IF(RANGWY.EQ.0.0)RETURN +C GET END POINTS IN X + IXF=IX0 + IXT=IX0+IBLIPH +C CALC AND PLOT POINTS + DO 20 I=1,NP +C PROPORTIONAL Y POSITIONS + YFF=FIRST+(I-1)*BIT + YWF=(YFF-YMIN)/RANGWY +C POSITION IN DRAWING BOARD UNITS + YDF=IY0+YWF*IYLEN + IYF=NINT(YDF) + CALL MOVEGR(IXF,IYF) + CALL DRAWGR(IXT,IYF) +20 CONTINUE + END IF + END + INTEGER FUNCTION IPSEP(XMAX,XMIN,NMAX) +C FIND SENSIBLE DEFAULT BLIP SEPARATION (SAY NMAX BLIPS PER PLOT) + D=XMAX-XMIN + IPSEP=D + IF(NMAX.LT.1)RETURN + NSEP=0 +1 CONTINUE + NSEP=NSEP+1 + D=D/NMAX + IF(D.GT.1.0)GO TO 1 + IPSEP=NMAX**(NSEP-1) + END + SUBROUTINE XHAIRS(XMAX,XMIN,YMAX,YMIN,IX0,IXLEN,IY0, + +IYLEN,ISXMAX,ISYMAX,IHX,IHY,NCHAR,KBOUT) +C AUTHOR: RODGER STADEN +C WRITE(KBOUT,*)' CROSS HAIRS' + CHARACTER TERM,COMMA + PARAMETER (COMMA=',') +C RANGE IN WORLD COORDS + RANGWX=XMAX-XMIN + IF(RANGWX.EQ.0.0)RETURN + RANGWY=YMAX-YMIN + IF(RANGWY.EQ.0.0)RETURN + XFF=IHX + YFF=IHY +C PROPORTIONAL X POSITIONS + XWF=(XFF-XMIN)/RANGWX +C PROPORTIONAL Y POSITIONS + YWF=(YFF-YMIN)/RANGWY +C POSITIONS IN DRAWING BOARD UNITS + XDF=IX0+XWF*IXLEN + YDF=IY0+YWF*IYLEN + IXF=NINT(XDF) + IYF=NINT(YDF) +10 CONTINUE + CALL XHAIR(IXF,IYF,TERM) +C RETURNS POSITION IN DB UNITS. CONVERT TO DRAWING BOARD UNITS +C RELATIVE POSITIONS + XFF=IXF-IX0 + YFF=IYF-IY0 + XFF=XFF/IXLEN + YFF=YFF/IYLEN +C CONVERT TO WORLD UNITS + XFW=XMIN+XFF*RANGWX + YFW=YMIN+YFF*RANGWY + IHX=NINT(XFW) + IHY=NINT(YFW) +C BACK TO VT100 MODE + CALL VT100M + WRITE(KBOUT,1014)IHX,IHY +1014 FORMAT(' Cross hair at',2I7) + IF(TERM.EQ.COMMA)GO TO 10 + END + SUBROUTINE LABLER(KBIN,KBOUT, + +ISXMAX,ISYMAX, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER LABEL*80,HELPF*(*) + CHARACTER LEFT,RIGHT,TERM,TUPPER + INTEGER CSIZE + EXTERNAL NOTIRL,TUPPER + PARAMETER (CSIZE=80) + PARAMETER (LEFT='L',RIGHT='R') +C FIDDLE TO CHECK ON GOING OFF SCREEN. ASSUME 80 CHARS PER SCREEN WIDTH +C NEED TO FIND WIDTH OF SCREEN IN DEVICE UNITS + CSCALE=ISXMAX/CSIZE + CALL CLEARV + WRITE(KBOUT,1004) +1004 FORMAT( + +' Type label then drive cross hair to left or right end',/, + +' of label position then hit "L" to write label left',/, + +' justified or "R" to write label right justified or',/, + +' the space bar to quit') + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0) RETURN +10 CONTINUE + CALL VT100M + CALL CLEARV + LENGTH = 0 + CALL GTSTR('Label',' ',LABEL,LENGTH,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + IF(LENGTH.EQ.0) RETURN +20 CONTINUE + CALL XHAIR(IHX,IHY,TERM) + TERM = TUPPER(TERM) + IF((TERM.EQ.RIGHT).OR.(TERM.EQ.LEFT))THEN + IF(TERM.EQ.RIGHT)IHX=NINT(REAL(IHX)-LENGTH*CSCALE) + IF((IHX.LT.1).OR.((IHX+LENGTH*CSCALE).GT.ISXMAX))GO TO 10 + CALL WRITET(IHX,IHY,LABEL,LENGTH) + GO TO 20 + END IF + GO TO 10 + END + SUBROUTINE CLIP(X1,XMAX,XMIN,Y1,YMAX,YMIN,X2,Y2) +C AUTHOR RODGER STADEN +C CLIPS COORDINATES IN USER UNITS + X2=MAX(X1,XMIN) + X2=MIN(X2,XMAX) + Y2=MAX(Y1,YMIN) + Y2=MIN(Y2,YMAX) + RETURN + END + SUBROUTINE XHAIRN(XMAX,XMIN,YMAX,YMIN,IX0,IXLEN,IY0, + +IYLEN,ISXMAX,ISYMAX,IHX,IHY,NCHAR,KBOUT, + +SEQ1,ISTAR1,IDIM1,SEQ2,ISTAR2,IDIM2,NSEQ) +C AUTHOR: RODGER STADEN +C WRITE(KBOUT,*)' CROSS HAIRS' + CHARACTER TERM,COMMA + PARAMETER (COMMA=',',ISPAN = 19) + CHARACTER SEQ1(IDIM1),SEQ2(IDIM2),MATCH(ISPAN) +C RANGE IN WORLD COORDS + RANGWX=XMAX-XMIN + IF(RANGWX.EQ.0.0)RETURN + RANGWY=YMAX-YMIN + IF(RANGWY.EQ.0.0)RETURN + XFF=IHX + YFF=IHY +C PROPORTIONAL X POSITIONS + XWF=(XFF-XMIN)/RANGWX +C PROPORTIONAL Y POSITIONS + YWF=(YFF-YMIN)/RANGWY +C POSITIONS IN DRAWING BOARD UNITS + XDF=IX0+XWF*IXLEN + YDF=IY0+YWF*IYLEN + IXF=NINT(XDF) + IYF=NINT(YDF) +10 CONTINUE + CALL XHAIR(IXF,IYF,TERM) +C RETURNS POSITION IN Db UNITS. CONVERT TO DRAWING BOARD UNITS +C RELATIVE POSITIONS + XFF=IXF-IX0 + YFF=IYF-IY0 + XFF=XFF/IXLEN + YFF=YFF/IYLEN +C CONVERT TO WORLD UNITS + XFW=XMIN+XFF*RANGWX + YFW=YMIN+YFF*RANGWY + IHX=NINT(XFW) + IHY=NINT(YFW) +C BACK TO VT100 MODE + CALL CLEARV + IF((TERM.EQ.'S').OR.(TERM.EQ.'s'))THEN + K1 = MAX(1,IHX) - ISTAR1 + 1 + K2 = MIN(IHX+ISPAN,IDIM1) - ISTAR1 + 1 + IF(NSEQ.NE.2)THEN + WRITE(KBOUT,1015)K1+ISTAR1-1,(SEQ1(K),K=K1,K2) +1015 FORMAT(' ',I7,' ',21A1) + ELSE IF(NSEQ.EQ.2)THEN + KK1 = MAX(1,IHY) - ISTAR2 + 1 + KK2 = MIN(IHY+ISPAN,IDIM2) - ISTAR2 + 1 + L = MIN(K2-K1,KK2-KK1) + 1 + IF(L.GT.0) THEN + CALL SQMTCH(SEQ1(K1),SEQ2(KK1),MATCH,L) + CALL FMT4LN(SEQ2(KK1),SEQ1(K1),MATCH,L, + + KK1+ISTAR2-1,K1+ISTAR1-1,KBOUT) + END IF + END IF + GO TO 10 + END IF + IF((TERM.EQ.'M').OR.(TERM.EQ.'m').AND.(NSEQ.EQ.2))THEN + K1 = MAX(1,IHX) - ISTAR1 + 1 + K2 = MIN(IHX+ISPAN,IDIM1) - ISTAR1 + 1 + KK1 = MAX(1,IHY) - ISTAR2 + 1 + KK2 = MIN(IHY+ISPAN,IDIM2) - ISTAR2 + 1 + L = MIN(K2-K1,KK2-KK1) + 1 + IF(L.GT.0) THEN + CALL DIABOX(SEQ2,IDIM2,SEQ1,IDIM1,KK1,K1,L,MATCH,KBOUT) + END IF + GO TO 10 + END IF + WRITE(KBOUT,1014)IHX,IHY +1014 FORMAT(' Cross hair at',2I7) + IF(TERM.EQ.COMMA)GO TO 10 + RETURN + END + SUBROUTINE DIABOX(SEQ1,IDIM1,SEQ2,IDIM2,I11,I21, + +LENGTH,LINE,KBOUT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM1),SEQ2(IDIM2),LINE(LENGTH) + INTEGER CTONUM + EXTERNAL CTONUM + WRITE(KBOUT,*) + I12 = I11 + LENGTH - 1 + I22 = I21 + LENGTH - 1 + WRITE(KBOUT,1000)(SEQ2(K),K=I21,I22) + DO 100 I=I12,I11,-1 + CALL FILLC(LINE,LENGTH,'.') + L=0 + DO 50 J=I21,I22 + L=L+1 + IF(CTONUM(SEQ1(I)).EQ.CTONUM(SEQ2(J))) + + LINE(L) = SEQ1(I) +50 CONTINUE + WRITE(KBOUT,1001)SEQ1(I),(LINE(K),K=1,L),SEQ1(I) +100 CONTINUE + WRITE(KBOUT,1000)(SEQ2(K),K=I21,I22) +1000 FORMAT(4X,36A1) +1001 FORMAT(3X,A1,36A1,A1) + END + SUBROUTINE RULER(J1,J2,IX0,IXLEN,IY0,IYLEN, + +ISXMAX,ISYMAX,KBIN,KBOUT,ISCALE, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + EXTERNAL IPSEP +C RULER DRAWS A SCALE ON THE X OR Y AXIS. THE CURRENT ACTIVE REGION IS +C J1,J2. THE USER CAN GIVE THE COORDINATE OF THE FIRST CHARACTER POSIITON +C TO BE MARKED (THE DEFAULT IS J1). THE SEPARATION AND HEIGHT IS ALSO +C DEFINED BY THE USER + IBH1=ISYMAX/100 + IF(ISCALE.EQ.2)IBH1=ISXMAX/100 + XMAX=J2 + XMIN=J1 + YMAX=J2 + YMIN=J1 + WRITE(KBOUT,1000) +1000 FORMAT(' Draw a scale') + MMN = J1 + MMX = J2 + ISTART = J1 + CALL GETINT(MMN,MMX,ISTART,'Put first tick at',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF((IOK.NE.0).OR.(IVAL.EQ.0)) THEN + CALL CLEARV + RETURN + END IF + ISTART = IVAL + FIRST=J1 +C SET DEFAULT NUMBER OF BLIPS TO 10 + NMAX=10 +C CHECK ON SEPARATION (NO CLOSER THAT 1 EVERY 5 PIXELS) + UMIN=ABS(XMIN-XMAX) + SMIN=IXLEN + ISEP=IPSEP(XMAX,XMIN,NMAX) + IF(ISCALE.EQ.2)THEN + UMIN=ABS(YMAX-YMIN) + SMIN=IYLEN + ISEP=IPSEP(YMAX,YMIN,NMAX) + END IF + IF(SMIN.EQ.0.0)THEN + WRITE(KBOUT,*)' ERROR IN SCREEN DEFINITION' + RETURN + END IF +C FIVE PIXELS IS + ONEPIX=UMIN/SMIN + IFIVEP=ONEPIX*5.0 +100 CONTINUE + MMN = IFIVEP + MMX = J2 - J1 + INC = ISEP + CALL GETINT(MMN,MMX,INC,'Tick spacing',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF((IOK.NE.0).OR.(IVAL.EQ.0)) THEN + CALL CLEARV + RETURN + END IF + INC = IVAL + BIT=INC + MMN = 0 + IF(ISCALE.EQ.1) MMX = IYLEN + IF(ISCALE.EQ.2) MMX = IXLEN + IBH = IBH1 + CALL GETINT(MMN,MMX,IBH,'Tick height',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF((IOK.NE.0).OR.(IVAL.EQ.0)) THEN + CALL CLEARV + RETURN + END IF + IBH = IVAL + IF(ISTART.GT.0)FIRST=ISTART + CALL CLEARV + CALL VECTOM + CALL SCALES(XMAX,XMIN,YMAX,YMIN, + +IX0,IXLEN,IY0,IYLEN,ISXMAX,ISYMAX, + +BIT,IBH,FIRST,ISCALE) + CALL VT100M + GO TO 100 + END + SUBROUTINE REDIR(IDEV,DEVOUT,IDEVGR,GORT,FILNAM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,KOPT) + CHARACTER HELPF*(*) +C AUTHOR: RODGER STADEN +C routine to allow the user to select disk output +C if GORT = 0 redirection off +C if GORT = 1 redirection of text +C if GORT = 2 redirection of graphics +C +C notice the redirection is handled differently for text and graphics: +C text is done by changing idev to devout and opening idev +C graphics is done by closing idevgr and opening devout and changing +C the value of idevgr in the graphics common block. Note also that +C IGORF is used by the graphics to suppress clearv and xhair +C +C Postscript output: file opened here, flag is posto: 0 off, 1 open +C All output controlled from c but implemented by modifying move +C and draw subroutines in pl4010 +C +C Can do one job at a time: if opening only one, if closing only one +C ie 2 visits to close both postcript and redirection +C if either is open and we come in to open the other we will first +C be asked if we want to close the open one +C + CHARACTER FILNAM*(*) + INTEGER DEVOUT,GORT,POSTO + PARAMETER (MAXPRM = 19) + CHARACTER PROMPT(3)*(MAXPRM) + CHARACTER FNDFIL*1024, FIL2*1024 + INTEGER OPENP + EXTERNAL FNDFIL, OPENP + SAVE POSTO + DATA POSTO/0/ + IF(GORT.NE.0) THEN + CALL YESNO(IN,'Close redirection file', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IN.EQ.0) THEN + IF(GORT.EQ.1) THEN + CLOSE(UNIT=DEVOUT) + IDEV = KBOUT + ELSE IF(GORT.EQ.2) THEN + CLOSE(UNIT=DEVOUT) + CALL OPENGR(IDEVGR) + END IF + GORT = 0 + RETURN + END IF + END IF + IF(POSTO.NE.0) THEN + CALL YESNO(IN,'Close postscript file', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IN.EQ.0) THEN + IJUNK=OPENP(' ',0,0,0,0,0,0) + POSTO = 0 + RETURN + END IF + END IF + IN = 1 + PROMPT(1) = 'Redirect text' + PROMPT(2) = 'Redirect graphics' + PROMPT(3) = 'Open postscipt file' + CALL RADION('Select redirection',PROMPT,3,IN, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IN.EQ.1) THEN + IF (GORT.NE.0) THEN + CALL ERROM(KBOUT, + + 'Output already redirected: please close file') + RETURN + END IF + FILNAM = ' ' + CALL OPENF1(DEVOUT,FILNAM,1,IOK,KBIN,KBOUT, + + 'Text file name',IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + IDEV=DEVOUT + GORT = 1 + ELSE IF(IN.EQ.2) THEN + IF (GORT.NE.0) THEN + CALL ERROM(KBOUT, + + 'Output already redirected: please close file') + RETURN + END IF + CLOSE(UNIT = IDEVGR) + FILNAM = ' ' + CALL OPENF1(DEVOUT,FILNAM,1,IOK,KBIN,KBOUT, + + 'Graphics file name',IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) THEN + CALL OPENGR(IDEVGR) + GORT = 0 + RETURN + END IF + CALL OPENGF(DEVOUT) + GORT = 2 + ELSE IF(IN.EQ.3) THEN + IF (POSTO.NE.0) THEN + CALL ERROM(KBOUT, + + 'Postscript already selected: please close file') + RETURN + END IF + 10 CONTINUE + FILNAM = ' ' + LIN = 0 + CALL GTSTR('Postcript file name',' ',FILNAM,LIN, + + KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + POSTO = 0 + IF ((INFLAG.EQ.2).OR.(LIN.EQ.0)) THEN + RETURN + END IF +CALL CROUTINE + IF (KOPT.EQ.1) THEN + CALL YESNO(LNDSCP, 'Print in portrait mode', + + IHELPS, IHELPE, HELPF, IDEVH, KBIN, KBOUT) + IF (LNDSCP.EQ.-1) THEN + RETURN + ENDIF + CALL GETINT(0, 9999, 0, 'Bottom left X co-ord of region', + + IX1, KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF (IOK.EQ.1) THEN + RETURN + ENDIF + CALL GETINT(0, 9999, 0, 'Bottom left Y co-ord of region', + + IY1, KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF (IOK.EQ.1) THEN + RETURN + ENDIF + CALL GETINT(IX1,9999,9999,'Top right X co-ord of region', + + IX2, KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF (IOK.EQ.1) THEN + RETURN + ENDIF + CALL GETINT(IY1,9999,9999,'Top right Y co-ord of region', + + IY2, KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF (IOK.EQ.1) THEN + RETURN + ENDIF + CALL GETINT(1,20,5,'Line thickness', + + IWIDTH, KBIN,KBOUT,IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF (IOK.EQ.1) THEN + RETURN + ENDIF + ELSE + LNDSCP = 0 + IX1 = 0 + IX2 = 9999 + IY1 = 0 + IY2 = 9999 + IWIDTH = 5 + END IF + FIL2 = FNDFIL(FILNAM) + POSTO = OPENP(FIL2, IX1, IY1, IX2, IY2, IWIDTH, LNDSCP) + END IF + END + SUBROUTINE SHUTD +C +C routine to contain all necessary shutdown procedures +C + IJUNK=OPENP(' ',0,0,0,0,0,0) + END + SUBROUTINE XHAIRR(ISXMAX,ISYMAX,IX,IY,TERM,DBTDUX,DBTDUY) + CHARACTER TERM + CALL XHAIR(IX,IY,TERM) + END + REAL FUNCTION CWORLD(I,I0,ILEN,RMIN,RMAX) + CWORLD = RMIN + (REAL(I-I0)/ILEN) * (RMAX-RMIN) + END + INTEGER FUNCTION NOPWIN(IY,IY0,IYLEN,MAXOPT) + INTEGER IY0(MAXOPT),IYLEN(MAXOPT) + DO 20 I = 29,MAXOPT + IF(IY.GT.IY0(I)) THEN + IF(IY.LT.(IY0(I)+IYLEN(I))) THEN + NOPWIN = I + RETURN + END IF + END IF +20 CONTINUE + NOPWIN = 0 + END + SUBROUTINE MOVEGR(IX,IY) +C +C +C +CALL CROUTINE + CALL MOVEP(IX,IY) + CALL MOVEXY(IX,IY) + END + SUBROUTINE DRAWGR(IX,IY) +C +C +C +CALL CROUTINE + CALL DRAWP(IX,IY) + CALL DRAWXY(IX,IY) + END + SUBROUTINE WRITET(IX,IY,CHARS,NCHARS) + CHARACTER CHARS*(*) +C +C +C +CALL CROUTINE + CALL WRITEP(IX,IY,CHARS,NCHARS) + CALL WRITXY(IX,IY,CHARS,NCHARS) + END + SUBROUTINE POINTG(IX,IY) +CALL C ROUTINE + CALL POINTP(IX,IY) + CALL DOTXY(IX,IY) + END + SUBROUTINE CLEARG +CALL C ROUTINE HERE + CALL CLEARP + CALL BLANKG + END diff --git a/src/staden/plotLog.c b/src/staden/plotLog.c new file mode 100644 index 0000000..260487a --- /dev/null +++ b/src/staden/plotLog.c @@ -0,0 +1,176 @@ +/* + Title: plotLog + + File: plotLog.c + Purpose: Manage a log of plotting commands + Last update: Thursday May 9 1991 +*/ + + +/* + The log is implemented using two files. The main (binary) one + simply stores the PLItem's whilst the auxilliary one stores + the text for text plotting commands. This split is essential + so that the items in the main file are all of the same size + and can thus be processed in bulk. This decision relies on the + fact that text plot commands are a tiny minority. + + As far as the abstraction of this module goes, the write pointer + is simply given by the end of file, the read pointer is held in + the file stream when `mode = reading'. +*/ + + + + +/* ---- Includes ---- */ + +#include "plotLog.h" +#include "fort.h" + +#include /* IMPORT: fclose, remove, fseek, fwrite, + fputc, fread, fgetc, + FILE, tmpnam, L_tmpnam, EOF, NULL */ + +#include /* IMPORT: Dimension */ + + + + +/* ---- Types ---- */ + + +typedef enum {reading, writing} Mode; + + + + +/* ---- Static variables ---- */ +#ifndef L_tmpnam +#define L_tmpnam 25 /* (sizeof(P_tmpdir) + 15) */ +#endif + +static char PLFileName[L_tmpnam]; +static char TFileName[L_tmpnam]; +static FILE *PLFileP; +static FILE *TFileP; +static Mode mode = writing; + + + + +/* ---- Exported functions ---- */ + + +PLResult CreatePL(void) +/* Create the log, which is initially empty. + Read and write pointers are set to the start. +*/ +{ /* Open the log files. + PlotLog - Update, truncated to empty, binary + Text - Update, truncated to empty, text + */ + PLFileP = fopen(tmpnam(PLFileName), "w+b"); + TFileP = fopen(tmpnam(TFileName), "w+"); + + if ((PLFileP==NULL) || (TFileP==NULL)) + return(CantCreate); + else + return(OK); +} + + +PLResult DestroyPL(void) +/* Delete the log. + Free up resources. +*/ +{ (void) fclose(PLFileP); + (void) remove(PLFileName); + (void) fclose(TFileP); + (void) remove(TFileName); + + return(OK); +} + + +PLResult ResetWritePL(void) +/* Reset the write pointer to the start of the log. + All previously written items are discarded. + The read pointer is left undefined. +*/ +{ mode = writing; + PLFileP = freopen(PLFileName, "w+b", PLFileP); + TFileP = freopen(TFileName, "w+", TFileP); + + if ((PLFileP==NULL) || (TFileP==NULL)) + return(Failed); + else + return(OK); +} + + +PLResult WritePL(PLItem i) +/* Append item `i' to the log. + The write pointer is incremented. + The read pointer is left undefined. +*/ +{ int_f x; + + if (mode == reading) + { mode = writing; + if (fseek(PLFileP, (off_t)0, 2) == EOF) return(Failed); + if (fseek(TFileP, (off_t)0, 2) == EOF) return(Failed); + } + + if (fwrite((char *) &i, sizeof(PLItem), 1, PLFileP) != 1) return(Failed); + + if (i.op == DrawStringOp) + { x = 0; + while (x < i.u.t.textl) + { if (fputc(i.u.t.textp[x], TFileP) == EOF) return(Failed); + x++; + } + } + + return(OK); +} + + +PLResult ResetReadPL() +/* Reset the read pointer to the start of the log. +*/ +{ mode = reading; + if (fseek(PLFileP, (off_t)0, 0) == EOF) return(Failed); + if (fseek(TFileP, (off_t)0, 0) == EOF) return(Failed); + + return(OK); +} + + +PLResult ReadPL(PLItem *i) +/* Read an item from the log. and return in `i'. + The field `i.u.t.textp' must point to a character array into which + any `DrawStringOp' parameter is copied. + Increment the read pointer. + If the read pointer was past the last item in the log, + then return `EndOfPL'. + + We must preserve the text pointer, because this will be overwritten + when we read the main record in. +*/ +{ char *textp=i->u.t.textp; + + if (fread((char *) i, sizeof(PLItem), 1, PLFileP) == 1) + { if (i->op == DrawStringOp) + { int_f x = 0; + i->u.t.textp = textp; + while (x < i->u.t.textl) + { if ((i->u.t.textp[x] = fgetc(TFileP)) == EOF) return(Failed); + x++; + } + } + return(OK); + } + else + return(EndOfPL); +} diff --git a/src/staden/plotLog.h b/src/staden/plotLog.h new file mode 100644 index 0000000..59c7a05 --- /dev/null +++ b/src/staden/plotLog.h @@ -0,0 +1,100 @@ +/* Title: plotLog + + File: plotLog.h + Purpose: Manage a log of plotting commands + Last update: Mon Mar 19th 1990 +*/ + + +/* + This module manages a single log of plotting commands. + + The structure of such commands is given by the PLItem type; + it is intended that the `PLOp op' item should indicate the + kind of operation, and the (x1,y1) position should indicate + where it starts. Further parameters may be required, depending + upon the operation; these are indicated by (x2,y2) or by `text'. + + createPL() must be called before any operation, and deletePL() + should be called at the end to release all resources used. + + The log maintains two pointers, a read pointer and a write pointer. + Items can be appended at the write pointer position with writePL() + and read from the read pointer position with readPL(). The + read and write pointers can be reset to the start of the log + using resetWritePL() and resetReadPL() respectively. + + The log can only be read sequentially, in a single sweep, because + the read pointer becomes undefined when anything is written. +*/ + + +#include /* IMPORT: Dimsension */ +#include "fort.h" + + +typedef enum +{ DrawLineOp, + DrawPointOp, + DrawStringOp +} PLOp; + + + +typedef struct +{ PLOp op; + Position x1, y1; + union {struct {Position x2, y2;} p2;/* For DrawLIneOp */ + struct {char *textp; /* For DrawStringOp */ + int_f textl;} t; + } u; +} PLItem; + + +typedef enum +{ OK, /* Normal result */ + EndOfPL, /* End of plot log reached. PLItem is undefined */ + CantCreate, /* The log could not be created */ + Failed /* Non-specific, fatal, error */ +} PLResult; + + +extern PLResult CreatePL(void); +/* Create the log, which is initally empty. + Read and write pointers are set to the start. +*/ + + +extern PLResult DestroyPL(void); +/* Delete the log. + Free up resources. +*/ + + +extern PLResult ResetWritePL(void); +/* Reset the write pointer to the start of the log. + All previously written items are discarded. + The read pointer is left undefined. +*/ + + +extern PLResult WritePL(PLItem i); +/* Append item `i'to the log. + The write pointer is incremented. + The read pointer is left undefined. +*/ + + +extern PLResult ResetReadPL(void); +/* Reset the read pointer to the start of the log. +*/ + + +extern PLResult ReadPL(PLItem *i); +/* Read an item from the log. and return in `i'. + The field `i.u.t.textp' must point to a character array into which + any `DrawStringOp' parameter is copied. + Increment the read pointer. + If the read pointer was past the last item in the log, + then return `EndOfPL'. +*/ diff --git a/src/staden/postscript.c b/src/staden/postscript.c new file mode 100644 index 0000000..48a6a13 --- /dev/null +++ b/src/staden/postscript.c @@ -0,0 +1,209 @@ +#include +#include +#include +#include +#include "FtoC.h" /* IMPORT: Fstr2Cstr */ +#include "postscript.h" +#include "fort.h" + +static FILE *psfp = NULL; /* pointer to ps output file */ +static int pspages; /* num of pages in current file */ +static int psupdated = 0; /* any updates since last 'showpage'? */ + +/* --- externable functions --- */ + +/* returns 1 for open, 0 for closed */ +int_f openp_x(char *FILE_p, + int_f *x1_p, int_f *y1_p, int_f *x2_p, int_f *y2_p, + int_f *width_p, int_f *lndscp_p, int_fl FILE_l) { + char fname[256]; + struct stat buf; + + Fstr2Cstr(FILE_p, FILE_l, fname, 256); + /* + * If given null filename then do nothing - gives us a chance to + * close the file without opening another! + */ + if (fname[0] == '\0') { + /*fprintf(stdout, "Closing PostScript output file\n");*/ + closep_x(); + return 0; + } + + /* + * Otherwise if we've already a file open then close it too before + * opening a new one. + */ + if (psfp != NULL) { + closep_x(); + psfp = NULL; + } + + /* now open our file */ + if (stat(fname, &buf) != -1) { + fprintf(stdout, "*** Error - file already exists\n"); + return 0; + } + if ((psfp = fopen(fname,"w")) == NULL) { + fprintf(stdout, + "*** Error - could not open postscript output file '%s'\n", + fname); + return 0; + } /*else + fprintf(stdout, "Sucessfully opened PostScript file '%s'.\n", fname); + */ + /* and write out the standard PostScript header */ + fputs("%!PS-Adobe-1.0\n\ +%%DocumentFonts: Times-Roman\n\ +%%For: Staden software package\n\ +%%Title: graphics_output.ps\n\ +%%Creator: Staden software package\n\ +%%CreationDate: (of header) 10/08/92\n\ +%%Pages: (atend)\n\ +%%EndComments\n\ +\n\ +% User tunable variables\n\ +", psfp); + fprintf(psfp, "/landscape %d def %% portrait vs landscape mode\n\ +/linewidth %2d def %% thickness of lines\n\ +\n\ +%% (x1,y1) must be bottom left and (x2,y2) must be top right\n\ +/x1 %4d def %% size and\n\ +/y1 %4d def %% location of the\n\ +/x2 %4d def %% box on graphics\n\ +/y2 %4d def %% window to draw\n\ +", *lndscp_p, *width_p, *x1_p, *y1_p, *x2_p, *y2_p); + fputs("\n\ +% location on postscript page\n\ +/inch {72 mul} def\n\ +/leftborder {.5 inch} def % left/right margin in inches\n\ +/botborder {.5 inch} def % top/bottom margin in inches\n\ +\n\ +% size of an A4 page\n\ +/pagew {11.75 inch} def\n\ +/pageh { 8.25 inch} def\n\ +\n\ +botborder leftborder\n\ +landscape 1 eq {\n\ + 90 rotate\n\ + /width {pagew botborder 2 mul sub} def\n\ + /height {pageh leftborder 2 mul sub} def\n\ + 0 -612 translate\n\ + exch\n\ +} {\n\ + /width {pageh botborder 2 mul sub} def\n\ + /height {pagew leftborder 2 mul sub} def\n\ +} ifelse translate\n\ +/conv {width 10000 div mul} def\n\ +\n\ +x2 x1 sub conv width div\n\ +y2 y1 sub conv height div\n\ +dup 3 2 roll dup 4 1 roll exch\n\ +\n\ +% centre drawing\n\ +dup 3 2 roll dup 4 1 roll exch\n\ +gt {width mul height exch sub 2 div 0 exch 3 -1 roll pop}\n\ + {pop width mul 2 div 0} ifelse pop pop 0 0 translate\n\ +\n\ +% scale drawing to large as possible, but still maintaining the aspect ratio\n\ +dup 3 2 roll dup 4 1 roll exch\n\ +gt {pop 1 exch div}\n\ + {1 exch div exch pop} ifelse dup scale\n\ +\n\ +x1 conv neg y1 conv neg translate\n\ +\n\ +% abbreiviations to shrink the drawing data\n\ +/s {stroke} def\n\ +/l {2 copy lineto s moveto} def\n\ +/m {moveto} def\n\ +/r {currentlinewidth 0 rlineto s} def\n\ +/p {showpage} def\n\ +/t {show} def\n\ +/n {newpath} def\n\ +/i {\n\ + /Times-Roman findfont 290 scalefont setfont\n\ + 2 setlinecap % square caps => equiv to square pixels\n\ + linewidth setlinewidth\n\ + 1 conv dup scale\n\ +} def\n\ +\n\ +%%EndProlog\n\ +", psfp); + pspages = 0; + psupdated = 0; + + return 1; +} + +void closep_x() { + if (psfp != NULL) { + /* do showpage etc if we've written any more since last showpage */ + if (psupdated) + clearp_x(); + fputs("%%Trailer\n", psfp); + fprintf(psfp, "%%%%Pages: %d\n", pspages); + fclose(psfp); + } +} + +void clearp_x() { + if (psfp != NULL) { + fputs("s\n", psfp); + fputs("p\n", psfp); + psupdated = 0; + } +} + +/* + * starts a new page - called when we do the first piece of PostScript + * after clearp_x(). + */ +static void psnext() { + if (psupdated == 0) { + psupdated = 1; + pspages++; + fprintf(psfp, "%%%%Page: %d %d\n", pspages, pspages); + fputs("i n\n", psfp); + } +} + +void movep_x(int_f *IX_p, int_f *IY_p) { + if (psfp) { + psnext(); + fprintf(psfp, "%d %d m\n", (int)*IX_p, (int)*IY_p); + } +} + +void drawp_x(int_f *IX_p, int_f *IY_p) { + if (psfp) { + psnext(); + fprintf(psfp, "%d %d l\n", (int)*IX_p, (int)*IY_p); + } +} + +void pointp_x(int_f *IX_p, int_f *IY_p) { + if (psfp) { + psnext(); + fprintf(psfp, "%d %d m\nr\n", (int)*IX_p, (int)*IY_p); + } +} + +void writep_x(int_f *IX_p, int_f *IY_p, char *TEXT_p, int_f *NCHAR_p, + int_fl TEXT_l) { + int_f len = *NCHAR_p, i; + char c; + + if (psfp) { + psnext(); + fprintf(psfp, "%d %d m\n(", (int)*IX_p, (int)*IY_p); + /* need to check for '\', '(' and ')' */ + for (i=0; iSpec.c. + + Menu creation and help facilities are included. +*/ + + +#include /* IMPORT: Widget, XtCallbackProc */ + + + + +extern void CreateProgMenus(Widget parentWid, + XtCallbackProc cbp, XtPointer client_data); +/* + Install the menus for this program into `parentWid'. + When pressed, each item will call `cbp' providing `client_data' + and the number of the item as `call_data'. +*/ + + + + +extern const int botHelpOpt; +extern const int topHelpOpt; +/* + The range of option numbers for the help system. +*/ + + +extern const char helpTextFN[]; +extern const char helpPtrsFN[]; +/* + File names for the help text and pointer files. +*/ + + +extern const char *helpTopics[]; +/* + Help topics, indexed in C between 0 and topHelpOpt-botHelpOpt + but referring to topics botHelpOpt to topHelpOpt +*/ diff --git a/src/staden/rep.f b/src/staden/rep.f new file mode 100644 index 0000000..89440ec --- /dev/null +++ b/src/staden/rep.f @@ -0,0 +1,954 @@ + SUBROUTINE FMAIN() +C +C rep compares a set of sequence readings against a library of alu +C sequences in both orientations. It creates 3 output files: +C a list of files that pass +C a list of files that fail +C a log file which contains the highest score found for each reading +C WARNING: rep also modifies all files in which it finds a match +C by writing a record that is translated by the assembly program into +C a tag to mark alu sequences. (First it copies the original file, say +C called fred, to fred.ALU then it modifies the original). +C The list of matching sequences includes: +C the file name, the top score, the top score for the other end of the read (0 +C if not above the cutoff), and the number of bases that does not appear to +C contain ALU. +C One assembly strategy would assemble the file of passes, and then sort the +C fail file on the last column so that the reads with the most nonALU sequence +C are at the top of the list, then assemble those. +C The log file is simply for checking the sensitivity of the program. +C + PARAMETER (MAXSEQ = 50000, + + MAXRD = 1000, + + MAXWLN = 4, + + LCONST = 4*MAXWLN, + + MAXWRD = 4**MAXWLN, + + MAXFIL = 150, + + MAXRSQ = 400, + + MAXCF = 4000, + + MAXDEV = 7, + + NAMLEN = 80) +C +C MAXFIL MAX NUMBER REPEAT FILES +C MAXRSQ MAX LENGTH OF EACH REPEAT FILE +C MAXCF MAX COMPARISON LENGTH +C + CHARACTER SEQV(MAXSEQ),SEQG(MAXRD) + INTEGER WORDP(MAXWRD,MAXFIL),POSN(MAXRSQ,MAXFIL) + REAL HIST(-MAXCF:MAXCF) + CHARACTER*(NAMLEN) NAMES(MAXFIL) + INTEGER STARTS(MAXFIL),MATRIX(5,5),RC,BESTRS(MAXRD),BESTRC(MAXRD) + REAL BESTSC(MAXRD) + CHARACTER BESTD(MAXRD) + INTEGER CONSTS(0:LCONST),DEVNOS(MAXDEV) + INTEGER SEQVI(MAXSEQ),SEQGI(MAXRD),SEQR(MAXRD) + CHARACTER*(NAMLEN) FILNAM,HELPF,NAME,TNAME,VNAME + INTEGER GNFFOF + EXTERNAL GNFFOF + PARAMETER (VNAME='.ALU') + ICG = 0 + ICB = 0 + ICB2 = 0 + IDM = 5 + CALL INITLU(IDM) + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + CALL MAKEID(MATRIX,IDM) + IDEVNI = DEVNOS(1) + IDEVV = DEVNOS(2) + IDEVNO = DEVNOS(3) + IDEVNF = DEVNOS(5) + IDEVNR = DEVNOS(6) + IDEVNL = DEVNOS(7) + WRITE(KBOUT,*)'rep v1.1: repeat examination program. April 93' + WRITE(KBOUT,*)'Author: Rodger Staden' + WRITE(KBOUT,*)'Copyright: Medical Research Council, UK' + FILNAM = ' ' + CALL OPENF1(IDEVNI,FILNAM,0,IOK,KBIN,KBOUT, + +'Input file of gel reading file names', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) STOP + FILNAM = ' ' + CALL OPENF1(IDEVNR,FILNAM,0,IOK,KBIN,KBOUT, + +'Input file of repeat file names', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) STOP + FILNAM = ' ' + CALL OPENF1(IDEVNO,FILNAM,1,IOK,KBIN,KBOUT, + +'Output file of passed file names', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) STOP + FILNAM = ' ' + CALL OPENF1(IDEVNF,FILNAM,1,IOK,KBIN,KBOUT, + +'Output file of failed file names', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) STOP + FILNAM = ' ' + CALL OPENF1(IDEVNL,FILNAM,1,IOK,KBIN,KBOUT, + +'Log file name', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) STOP + MN = 2 + MX = MAXWLN + LENGTH = 4 +C CALL GETINT(MN,MX,LENGTH, +C +'Word length', +C +IVAL,KBIN,KBOUT, +C +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C IF(IOK.NE.0) STOP +C LENGTH = IVAL + MN = 1 + MX = 11 + LW = 7 +C CALL GETINT(MN,MX,LW, +C +'Number of diagonals to combine', +C +IVAL,KBIN,KBOUT, +C +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C IF(IOK.NE.0) STOP +C LW = IVAL + XMN = 0.0 + XMX = LW*1.0 + XMX = 1. + CUT = 0.6 + CALL GETRL(XMN,XMX,CUT, + +'Cutoff score', + +VAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) STOP + CUT = VAL +C +C set initial values so we hash the first vector +C + ICSITT = 0 + IPSITT = 0 + ICSITE = ICSITT + IPSITE = IPSITT + IDE = (IDM-1)**LENGTH + CALL SETCN(CONSTS,LENGTH,IDM,LCONST) + CALL GETSQS(IDEVNR,IDEVV,SEQV,MAXSEQ,STARTS,NAMES, + +FILNAM,NFILE,MAXFIL,MAXNAM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + IDIMV = STARTS(NFILE+1)-1 + NFILES = NFILE + 1 + CALL CONNUM(SEQV,SEQVI,IDIMV) + DO 3 NSEQ = 1,NFILE + IS = STARTS(NSEQ) + IE = STARTS(NSEQ+1) - 1 + IDIMV = IE - IS + 1 +C WRITE(*,*)(SEQVI(K),K=IS,IE) + CALL ENCONC(SEQVI(IS),IDIMV,POSN(1,NSEQ),WORDP(1,NSEQ), + + IDE,IDM,CONSTS,LENGTH, + + LCONST) + 3 CONTINUE + CALL BLATA(WORDP,IDE,MAXFIL,LENGTH,NSEQ,CONSTS,LCONST) +C CALL LISTN(SEQV,MAXSEQ,STARTS,NFILE,60,1,200,IDEV,KBOUT, +C +NAMES,MAXFIL) + IDEVE = 0 + IDEVG = IDEVV + MINR = 20 + JGEL = 0 + 10 CONTINUE +C Get next experiment file name + IOK = GNFFOF(IDEVNI,NAME) + IF(IOK.EQ.1) THEN + WRITE(KBOUT,*) + + 'Finished after processing',JGEL,' files and finding' + WRITE(KBOUT,*)ICG,' free of the repeat' + WRITE(KBOUT,*)ICB,' contain the repeat' + WRITE(KBOUT,*)ICB2,' contain the repeat at both ends' + STOP + ELSE IF(IOK.EQ.2) THEN + CALL ERROM(KBOUT,'Empty line in file of file names') + GO TO 10 + ELSE IF(IOK.EQ.3) THEN + CALL ERROM(KBOUT,'Error reading file of file names') + GO TO 10 + END IF + IDIMG = MAXRD + CALL OPENRS(IDEVG,NAME,IOK,LRECL,2) + CALL ARRFIM(IDEVG,SEQG,IDIMG,KBOUT) + IF (IDIMG.GT.MINR) THEN + JGEL = JGEL + 1 + CALL CONNUM(SEQG,SEQGI,IDIMG) + CALL VCUTT(SEQR, + + POSN,WORDP,IDE,MAXRSQ,MAXFIL,SEQGI,IDIMG,CONSTS, + + LENGTH,IDM,LCONST,HIST,MAXCF,KBOUT,CUT,LC,RC, + + STARTS,NFILES,LW, + + IDEVNF,IDEVNO,IDEVNL, + + NAME,NAMES,MATRIX,SEQVI,MAXSEQ,SCORE,ICG,ICB, + + ICB2, + + BESTRS,BESTRC,BESTSC,BESTD,MAXRD,KCUT,LCUT,EMAX) +C write(*,*)lc,rc + IF(LC.EQ.0) THEN + CLOSE(UNIT=IDEVG) + ELSE + CALL VPOUTR(IDEVG,IDEVNR,NAME,LC,RC, + + SCORE,VNAME,TNAME,KCUT,LCUT,EMAX,IOK) + IF(IOK.NE.0) STOP + END IF + END IF + GO TO 10 + END + SUBROUTINE SQCOMI(SEQ,ID) + INTEGER SEQ(ID),COMP(5) + SAVE COMP + DATA COMP/3,4,1,2,5/ + DO 10 I=1,ID + SEQ(I) = COMP(SEQ(I)) + 10 CONTINUE + END + SUBROUTINE SQREVI(SEQ,ID) + INTEGER SEQ(ID) + IEND = ID/2 + DO 100 I=1,IEND + J = SEQ(I) + SEQ(I) = SEQ(ID+1-I) + SEQ(ID+1-I) = J + 100 CONTINUE + END + SUBROUTINE VCUTT(SEQR, + +POSN,WORDP,IDE,MAXRSQ,MAXFIL,SEQH,IDIMH,CONSTS, + +LENGTH,IDM,LCONST,HIST,MAXSEQ,KBOUT,CUT,ICUT,JCUT, + +STARTS,NFILES,LW, + +IDEVNF,IDEVNO,IDEVNL, + +NAME,NAMES,MATRIX,SEQV,MAXVSQ,DMAX,ICG,ICB,ICB2, + +BESTRS,BESTRC,BESTSC,BESTD,MAXRD,KCUT,LCUT,EMAX) + INTEGER SEQH(IDIMH),STARTS(NFILES),SEQR(IDIMH),RC,MATRIX(IDM,IDM) + INTEGER POSN(MAXRSQ,MAXFIL),WORDP(IDE,MAXFIL),CONSTS(0:LCONST) + REAL HIST(-MAXSEQ:MAXSEQ) + INTEGER BESTRS(MAXRD),BESTRC(MAXRD),SEQV(MAXVSQ) + REAL BESTSC(MAXRD) + CHARACTER BESTD(MAXRD) + CHARACTER NAME*(*),ORIEN*9 + CHARACTER*(*) NAMES(NFILES) +C WRITE(*,*)IDIMH + CALL COPYI(SEQH,SEQR,IDIMH) + CALL SQREVI(SEQR,IDIMH) + CALL SQCOMI(SEQR,IDIMH) + CALL FILLI(BESTRS,IDIMH,0) + CALL FILLI(BESTRC,IDIMH,0) + CALL FILLR(BESTSC,IDIMH,0) + CALL FILLC(BESTD,IDIMH,'F') + DMAX = -1. +C WRITE(*,*)NFILES + IBLANK = INDEX(NAME,' ') + DO 30 NSEQ = 1,NFILES-1 + IS = STARTS(NSEQ) + IE = STARTS(NSEQ+1) - 1 + IDIMV = IE - IS + 1 + IDIMHT = IDIMH + IBLANK = INDEX(NAME,' ') + JBLANK = INDEX(NAMES(NSEQ),' ') + CALL VCUT(IDIMV,POSN(1,NSEQ),WORDP(1,NSEQ),MAXRSQ, + + IDE,SEQH,IDIMHT,CONSTS, + + LENGTH,IDM,LCONST,HIST,MAXSEQ,KBOUT,CUT,LC,RC,LW,DSCORE, + + SDS,MATRIX,SEQV(STARTS(NSEQ))) +C WRITE(*,*)CUT,DMAX,DSCORE + IF(DSCORE.GT.CUT) THEN + IF(DSCORE.GT.BESTSC(LC)) THEN + BESTSC(LC) = DSCORE + BESTRC(LC) = RC + BESTRS(LC) = NSEQ + END IF + END IF + IF(DSCORE.GT.DMAX) THEN + DMAX = DSCORE + ORIEN = 'Left' + NBSEQ = NSEQ + ICUT = LC + JCUT = RC + END IF + CALL VCUT(IDIMV,POSN(1,NSEQ),WORDP(1,NSEQ),MAXRSQ, + + IDE,SEQR,IDIMHT,CONSTS, + + LENGTH,IDM,LCONST,HIST,MAXSEQ,KBOUT,CUT,LC,RC,LW,DSCORE, + + SDS,MATRIX,SEQV(STARTS(NSEQ))) +C WRITE(*,*)DMAX,DSCORE + IF(DSCORE.GT.CUT) THEN + JCUT = IDIMH - LC + 1 + ICUT = IDIMH - RC + 1 + LC = ICUT + RC = JCUT + IF(DSCORE.GT.BESTSC(LC)) THEN + BESTSC(LC) = DSCORE + BESTRC(LC) = RC + BESTRS(LC) = NSEQ + BESTD(LC) = 'R' + END IF + END IF + IF(DSCORE.GT.DMAX) THEN + DMAX = DSCORE + ORIEN = 'Rev' + NBSEQ = NSEQ + JCUT = IDIMH - LC + 1 + ICUT = IDIMH - RC + 1 + END IF + 30 CONTINUE + IF(DMAX.LT.CUT) THEN + EMAX = 0. + JBLANK = INDEX(NAMES(NBSEQ),' ') + WRITE(IDEVNO,1001)NAME(1:IBLANK),DMAX,EMAX,IDIMH + 1001 FORMAT(A,2F5.2,I5) + WRITE(IDEVNL,1000)NAME(1:IBLANK),NAMES(NBSEQ)(1:JBLANK), + + ORIEN,ICUT,JCUT,IDIMH,DMAX + ICG = ICG + 1 + ICUT = 0 + RETURN + END IF +C +C find max value +C + DMAX = CUT + DO 40 I=1,IDIMH + IF(BESTSC(I).GT.DMAX) THEN + DMAX = BESTSC(I) + ICUT = I + JCUT = BESTRC(I) + NBSEQ = BESTRS(I) + IF(BESTD(I).EQ.'F') THEN + ORIEN = 'Left' + ELSE + ORIEN = 'Rev' + END IF + END IF + 40 CONTINUE + JBLANK = INDEX(NAMES(NBSEQ),' ') + WRITE(IDEVNL,1000)NAME(1:IBLANK),NAMES(NBSEQ)(1:JBLANK), + + ORIEN,ICUT,JCUT,IDIMH,DMAX + 1000 FORMAT(A,' ',A,' ',A,3I6,F7.3) + ICB = ICB + 1 +C +C now find best value in biggest bit thats left +C + EMAX = CUT + IF(ICUT.GT.IDIMH-JCUT) THEN + LEFT = ICUT - 1 + DO 50 I=1,ICUT-1 + IF((BESTSC(I).GT.EMAX).AND.(BESTRC(I).LT.ICUT)) THEN + EMAX = BESTSC(I) + KCUT = I + LCUT = BESTRC(I) + MBSEQ = BESTRS(I) + LEFT = KCUT - 1 + IF(BESTD(I).EQ.'F') THEN + ORIEN = 'Left' + ELSE + ORIEN = 'Rev' + END IF + END IF + 50 CONTINUE + ELSE + LEFT = IDIMH - JCUT + DO 60 I=JCUT,IDIMH + IF(BESTSC(I).GT.EMAX) THEN + EMAX = BESTSC(I) + KCUT = I + LCUT = BESTRC(I) + MBSEQ = BESTRS(I) + LEFT = IDIMH - LCUT + IF(BESTD(I).EQ.'F') THEN + ORIEN = 'Left' + ELSE + ORIEN = 'Rev' + END IF + END IF + 60 CONTINUE + END IF + IF(EMAX.GT.CUT) THEN + JBLANK = INDEX(NAMES(MBSEQ),' ') + WRITE(IDEVNL,1000)NAME(1:IBLANK),NAMES(MBSEQ)(1:JBLANK), + + ORIEN,KCUT,LCUT,IDIMH,EMAX + WRITE(IDEVNF,1001)NAME(1:IBLANK),DMAX,EMAX,LEFT + ICB2 = ICB2 + 1 + ELSE + EMAX = 0. + WRITE(IDEVNF,1001)NAME(1:IBLANK),DMAX,EMAX,LEFT + KCUT = 0 + END IF + END + SUBROUTINE VCUT(IDIMV, + +POSN,WORDP,MAXRSQ,IDE,SEQH,IDIMH,CONSTS, + +LENGTH,IDM,LCONST,HIST,MAXSEQ,KBOUT,CUT,LC,RC,LW,DSCORE, + +SDS,MATRIX,SEQV) + INTEGER SEQH(IDIMH),RC,MATRIX(IDM,IDM),SEQV(IDIMV) + INTEGER POSN(MAXRSQ),WORDP(IDE),CONSTS(0:LCONST) + REAL HIST(-MAXSEQ:MAXSEQ) + EXTERNAL NCODEA +C WRITE(*,*)'VCUT' +C WRITE(*,*)(SEQH(KKK),KKK=1,IDIMH) +C WRITE(*,*)(SEQV(KKK),KKK=1,IDIMV) +C +C when we get here idimh is the length we want to screen +C + CALL FILLR(HIST(LENGTH-IDIMV),IDIMH+IDIMV+1,0.) + DO 20 I = 1,IDIMH-LENGTH+1 + J = NCODEA(SEQH(I),LENGTH,CONSTS,IDM,LCONST) + IF(J.NE.0)THEN + J1 = WORDP(J) +C write(*,*)i,seqh(i),j,j1 + IF(J1.NE.0)THEN + K = I - J1 + HIST(K) = HIST(K) + 1. +10 CONTINUE + J2 = J1 + J1 = POSN(J2) + IF(J1.NE.0)THEN + K = I - J1 + HIST(K) = HIST(K) + 1. + GO TO 10 + END IF + END IF + END IF +20 CONTINUE +C I1 = LENGTH - IDIMV +C I2 = IDIMH - LENGTH +C DO 55 IJK=I1,I2 +C WRITE(33,*)IJK,HIST(IJK) +C 55 CONTINUE + CALL PHIST(HIST,IDIMV,IDIMH,LENGTH,MAXSEQ) +C DO 56 IJK=I1,I2 +C WRITE(33,*)IJK,HIST(IJK) +C 56 CONTINUE + CALL FCUT(HIST,IDIMV,IDIMH,SEQV,SEQH,LENGTH, + + MAXSEQ,CUT,LC,RC,LW,DSCORE,SDS,MATRIX,IDM) + END + SUBROUTINE PHIST(HIST,IDIMV,IDIMH,LENGTH,MAXSEQ) + REAL HIST(-MAXSEQ:MAXSEQ) +C +C modified 29-3-93 to correct for diagonal length being shorter by length -1 +C + IF(IDIMV.GE.IDIMH) THEN + D = 1 + DO 10 I=LENGTH-IDIMV,IDIMH-IDIMV-1 + HIST(I) = HIST(I)/D + D = D + 1 + 10 CONTINUE + D = IDIMH - LENGTH + 1 + DO 20 I=IDIMH-IDIMV,0 + HIST(I) = HIST(I)/D + 20 CONTINUE + D = IDIMH - 1 - LENGTH + 1 + DO 30 I=1,IDIMH-LENGTH + HIST(I) = HIST(I)/D + D = D - 1 + 30 CONTINUE + ELSE + D = 1 + DO 40 I=LENGTH-IDIMV,-1 + HIST(I) = HIST(I)/D + D = D + 1 + 40 CONTINUE + D = IDIMV - LENGTH + 1 + DO 50 I=0,IDIMH-IDIMV + HIST(I) = HIST(I)/D + 50 CONTINUE + D = IDIMV - 1 - LENGTH + 1 + DO 60 I=IDIMH-IDIMV+1,IDIMH-LENGTH + HIST(I) = HIST(I)/D + D = D - 1 + 60 CONTINUE + END IF + END + SUBROUTINE FCUT(HIST,IDIMV,IDIMH,SEQV,SEQH,LENGTH, + + MAXSEQ,CUT,LC,RC,LW,D,SDS,MATRIX,IDM) + REAL HIST(-MAXSEQ:MAXSEQ) + INTEGER RC,SEQH(IDIMH),MATRIX(IDM,IDM),SEQV(IDIMV) + PARAMETER (MINDIA = 25) + REAL DSCAN + EXTERNAL DSCAN +C +C dont look within mindia of edges +C +C and rotate over a window of length lw +C + LB = 1 + LW/2 + LF = LW/2 + I1 = LENGTH - IDIMV + MINDIA + I2 = IDIMH - LENGTH - MINDIA +C first we do the first window length + DT = 0. + DO 1 I=I1-LB,I1+LF-1 + DT = DT + HIST(I) + 1 CONTINUE + LC = 0 + RC = 0 + DMAX = 0. + ID = 0 +C WRITE(*,*)'LOOKING AT ',I1,I2 +C RMSQ = 0. +C RM = 0. +C write(*,*)i1,i2 + DO 10 I=I1,I2 + DT = DT - HIST(I-LB) + HIST(I+LF) +C DT = HIST(I) +C WRITE(*,*)I,DT +C RM = RM + DT +C RMSQ = RMSQ + DT*DT + IF(DT.GT.DMAX) THEN + DMAX = DT + ID = I +C write(*,*)'i,dt',i,dt + END IF + 10 CONTINUE +C N = I2 - I1 +C RM = RM / N +C RMSQ = RMSQ / N +C RM2 = RM * RM +C SD = 0. +C T = RMSQ - RM2 +C IF (T.GT.0.) SD = SQRT(T) +C SDS = (DMAX - RM ) / SD +C D = DMAX +C WRITE(*,*)'Best diagonal, score and local sum',ID,DMAX,D +C WRITE(*,1000)ID,DMAX,D +C 1000 FORMAT(I6,2F10.3) +C IF(D.LT.CUT) RETURN + IF(IDIMV.GE.IDIMH) THEN + IF(ID.GE.0) THEN + LC = ID + 1 + RC = IDIMH + ELSE + LC = 1 + RC = MIN(IDIMH,IDIMV + ID) + END IF + ELSE + IF(ID.GE.0) THEN + LC = ID + 1 + RC = MIN(ID+IDIMV,IDIMH) + ELSE + LC = 1 + RC = ID + IDIMV + END IF + END IF +C WRITE(*,*)' Discard ',LC, ' to ',RC + LS = 5 + MS = 4 + CALL DIAGO(ID,IX,IY) +C write(*,*)'ix,iy',ix,iy + D = DSCAN(SEQH,IDIMH,IX,SEQV,IDIMV,IY,LW,LS,MATRIX,IDM,MS) +C WRITE(*,*)IX,IY,D + END + INTEGER FUNCTION GNFFOF(IDEV,NAME) + CHARACTER NAME*(*) + EXTERNAL NOTLR +C +C routine to read a file of file names and return a name +C deals with leading spaces and trims names at first space +C after name: eg ' fred is a bum' is returned as 'fred' +C needed because file names can contain spaces (not our file names!) +C and the open statement expects the names to match precisely +C +C return 0 = ok, 2 = empty line in file, 3 = error in read, 1 = end of file +C + READ(IDEV,1000,ERR=100,END=200)NAME + 1000 FORMAT(A) +C +C get first non space position +C + LENGTH = LEN(NAME) + I = NOTLR(NAME,LENGTH,' ') +C empty line ? + IF(I.EQ.0) THEN + GNFFOF = 2 + RETURN + END IF +C now want first space after I + J = INDEX(NAME(I+1:),' ') + IF(J.EQ.0) THEN + J = LENGTH + ELSE + J = J + I - 1 + END IF + CALL SHFTLS(NAME,I,1,J) + NAME(J-I+2:) = ' ' + GNFFOF = 0 + RETURN + 100 CONTINUE + GNFFOF = 3 + RETURN + 200 CONTINUE + GNFFOF = 1 + END + SUBROUTINE SHFLCA(STRING,MAXAR,FROMS,TO,FROME) + CHARACTER STRING(MAXAR) + INTEGER FROMS,FROME,TO +C +C shift left from from to to +C + J = TO + DO 10 I=FROMS,FROME + STRING(J) = STRING(I) + J = J + 1 + 10 CONTINUE + END + SUBROUTINE AERROR(IDEVS,IDEVF,NAME,IERR) + CHARACTER NAME*(*) +C +C handle errors for assembly +C +C errors are: +C 0 file not found +C 1 read too short +C 2 failed to align and not entered +C 3 failed on entry +C 4 failed to align but entered + WRITE(IDEVF,1000)NAME(1:INDEX(NAME,' ')),IERR + 1000 FORMAT(A,I2) + CALL ERROM(IDEVS,'Failed reading written to error file') + END + SUBROUTINE GETSQS(IDEV1,IDEV2,SEQ,MAXSEQ,STARTS,NAMES, + +FILNAM,NFILE,MAXFIL,MAXNAM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER SEQ(MAXSEQ),HELPF*(*) + CHARACTER*(*) FILNAM,NAMES(MAXFIL) + INTEGER STARTS(MAXFIL) +C +C concatenate sequences into seq +C number of them is nfile +C sequence i starts at starts(i) and has file name names(i) +C and starts(nfile+1) is the position of the first unused element + NFILE = 0 + IEND = 0 + FILNAM = ' ' +C CALL OPENF1(IDEV1,FILNAM,0,IOK,KBIN,KBOUT, +C +'File of file names', +C +IHELPS,IHELPE,HELPF,IDEVH) +2 CONTINUE + IF (NFILE.LT.MAXFIL) THEN + READ(IDEV1,1000,END=3)FILNAM + 1000 FORMAT(A) +C WRITE(*,*)NFILE,IEND,FILNAM + NFILE = NFILE + 1 + STARTS(NFILE) = IEND + 1 + NAMES(NFILE) = FILNAM + IDIM2 = MAXSEQ - IEND + IF (IDIM2.GT.0) THEN + CALL OPENRS(IDEV2,FILNAM,IOK,LRECL,2) + CALL ARRFIL(IDEV2,SEQ(STARTS(NFILE)),IDIM2,KBOUT) + CLOSE(UNIT=IDEV2) + IEND = IEND + IDIM2 + GO TO 2 + END IF +C +C if we get here weve run out of array space +C + NFILE = NFILE - 1 + WRITE(KBOUT,*)'Only ',NFILE,' files read: no more memory' + CLOSE(UNIT=IDEV1) + RETURN + ELSE +C +C if we get here weve got too many files +C + NFILE = NFILE - 1 + CLOSE(UNIT=IDEV1) + WRITE(KBOUT,*)'Maximum files ',NFILE,' read' + RETURN + END IF + 3 CONTINUE + STARTS(NFILE+1) = IEND + 1 + CLOSE(UNIT=IDEV1) + END + SUBROUTINE LISTN(SEQ,MAXCHR,LENSEQ,NFILE,LINLEN,I1,I2, + +IDEV,KBOUT,NAMES,MAXFIL) + CHARACTER*(*) NAMES(MAXFIL) + PARAMETER (MAXLIN = 120) + CHARACTER SEQ(MAXCHR),LINE*(MAXLIN) + INTEGER LENSEQ(MAXFIL) + EXTERNAL NOTIRL +C SET WIDTH FOR LAST PAGE + LPAGE=MOD(I2-I1+1,LINLEN) +C HOW MANY PAGE WIDTHS? + NPAGE=1+(I2-I1+1)/LINLEN + IF(MOD(I2-I1+1,LINLEN).EQ.0)THEN + NPAGE=NPAGE-1 + LPAGE=LINLEN + END IF + ISTART=I1-LINLEN + DO 50 I=1,NPAGE + ISTART=ISTART+LINLEN + IF(I.EQ.NPAGE)LINLEN=LPAGE + WRITE(IDEV,1006)(K,K=ISTART+9,ISTART+LINLEN-1,10) + DO 40 J=1,NFILE + KF = ISTART + LENSEQ(J) - 1 + KT = MIN(KF+LINLEN,LENSEQ(J+1)) - 1 + LINE(1:) = ' ' + WRITE(LINE,1003,ERR=60)(SEQ(K),K=KF,KT) +1003 FORMAT(' ',60A1) + LINE(KT-KF+4:) = NAMES(J)(1:15) + WRITE(IDEV,1004,ERR=60)LINE(1:NOTIRL(LINE,MAXLIN,' ')) + 1004 FORMAT(A) +1006 FORMAT(' ',10I10) +40 CONTINUE + WRITE(IDEV,1008) +1008 FORMAT( ) +50 CONTINUE + RETURN +60 CONTINUE + WRITE(KBOUT,*)' Error writing file' + END +C SUBROUTINE TO READ CHARACTER DATA FROM IDEV, REMOVE SPACES, FILL +C ARRAY AND RETURN NUMBER OF ELEMENTS USED. ANY LINES STARTING WITH +C A ; ARE TREATED AS COMMENTS + SUBROUTINE ARRFIM(IDEV,SEQNCE,J,KBOUT) +C 14-8-91 Added err= option to read, and set length to 0 if error found +C AUTHOR: RODGER STADEN + CHARACTER TEMP(80),SEQNCE(J) + CHARACTER SPACE,ENDCHR,TITCHR + SAVE ENDCHR,SPACE,TITCHR + DATA ENDCHR/'@'/ + DATA SPACE/' '/ + DATA TITCHR/';'/ + IDMX=J + J=0 +1 CONTINUE + READ(IDEV,1001,END=30,ERR=40)TEMP +1001 FORMAT(80A1) + IF(TEMP(1).EQ.TITCHR)THEN +C WRITE(KBOUT,1003)(TEMP(K),K=2,80) +C1003 FORMAT(' ',79A1) + GO TO 1 + END IF +10 CONTINUE + DO 20 I=1,80 + IF(TEMP(I).NE.SPACE)THEN + IF(TEMP(I).EQ.ENDCHR)RETURN + IF(J.EQ.IDMX)THEN + WRITE(KBOUT,1002)IDMX +1002 FORMAT( + + ' Too much data. Maximum possible', + + ' =',I6,', input stopped there') + RETURN + END IF + J=J+1 + SEQNCE(J)=TEMP(I) + END IF +20 CONTINUE + GO TO 1 +30 CONTINUE + RETURN + 40 CONTINUE + CALL ERROM(KBOUT,'Error reading file') + J = 0 + END + SUBROUTINE BLATA(WORDP,IDE,MAXFIL,LENGTH,NSEQ,CONSTS,LCONST) + INTEGER WORDP(IDE,MAXFIL) + INTEGER SEQA(4),CONSTS(0:LCONST) + EXTERNAL NCODEA +C +C fudge: set xyxy to 0 +C + DO 10 I = 1,4 + DO 10 J = 1,4 + SEQA(1) = I + SEQA(3) = I + SEQA(2) = J + SEQA(4) = J + JA = NCODEA(SEQA,LENGTH,CONSTS,5,LCONST) + DO 5 K = 1,NSEQ + WORDP(JA,K) = 0 + 5 CONTINUE + 10 CONTINUE + END + SUBROUTINE MAKEID(MATRIX,IDM) + INTEGER MATRIX(IDM,IDM) +C +C set main diagonal to 1 except idm,idm +C + DO 10 I=1,IDM + DO 10 J=1,IDM + IF(I.EQ.J) THEN + MATRIX(I,J) = 1 + ELSE + MATRIX(I,J) = 0 + END IF + 10 CONTINUE + MATRIX(IDM,IDM) = 0 + END + SUBROUTINE DIAGO(D,I,J) + INTEGER D + IF(D.GE.0) THEN + J = 1 + I = D + 1 + ELSE + I = 1 + J = ABS(D) + 1 + END IF + END + REAL FUNCTION DSCAN(SX,LX,PXIN,SY,LY,PYIN,NDD, + +LW,SCORES,MAXC,MS) +C seqs sx,sy length lx,ly positions px,py +C number of diagonals nd +C length of windw lw +C min score ms + PARAMETER (MAXD = 21) + INTEGER SX(LX),PXIN,PYIN,SCORES(MAXC,MAXC),SY(LY) + INTEGER PX,PY,CS(-MAXD:MAXD),CX(-MAXD:MAXD),CY(-MAXD:MAXD) + PX = PXIN + PY = PYIN +C WRITE(*,*)'PXIN,PYIN,ND,LW,MS,MAXC,MAXW', +C +PXIN,PYIN,ND,LW,MS,MAXC +C +C new scheme for choosing number of diagonals and reducing chnace +C of finding matches close to edge. Let the number of diagonals nd +C depend on the length of the diagonal we are going to scan along. +C <29 nd = 3, >269 = 15 +C + L = MIN(LX-PX,LY-PY) + IF(L.LT.31) THEN + ND = 3 + ELSE + ND = MIN(MAXD,3+6*((L-30)/40)) + END IF +C WRITE(*,*)L,ND + ND2 = ND/2 +C +C get start safely positioned by shifting the original p's +C + I = 0 + IF(PX.LT.ND2+1) I = ND2 - PX + 1 + PX = PX + I + PY = PY + I +C WRITE(*,*)PX,PY +C +C set current x +C + DO 10 I=-ND2,ND2 + CX(I) = PX + I + CY(I) = PY + 10 CONTINUE +C WRITE(*,*)(CX(K),CY(K),K=-ND2,ND2) + DO 33 I=-MAXD,MAXD + CS(I) = 0 + 33 CONTINUE +C CALL FILLI(CS(-ND2),ND,0) +C +C do the first window length +C +C WRITE(*,*)(SY(K),K=1,10) + DO 30 J=0,LW-1 + DO 20 I=-ND2,ND2 +C WRITE(*,*)'J,I,I+J,CX,CY,SX,SY',J,I,I+J,CX(I)+J,CY(I)+J, +C + SX(CX(I)+J),SY(CY(I)+J) +C WRITE(*,*)CX(I)+J,CY(I)+J +C WRITE(*,*)SX(CX(I)+J),SY(CY(I)+J) + CS(I) = CS(I) + SCORES(SX(CX(I)+J),SY(CY(I)+J)) + 20 CONTINUE + 30 CONTINUE +C WRITE(*,*)CS + IDSCAN = 0 + IS = IMAXA(CS(-ND2),ND) + IF(IS.GE.MS) DSCAN = DSCAN + 1 + NSTEPS = MIN(LX - (PX+LW-1+ND2),LY - (PY+LW-1+ND2)) +C NSTEPS = MIN(LX - (PX+LW-1),LY - (PY+LW-1+ND2)) +C WRITE(*,*)'NSTEPS',NSTEPS + DO 50 J=1,NSTEPS + DO 40 I=-ND2,ND2 + ICX = CX(I) + ICY = CY(I) + CS(I) = CS(I) - SCORES(SX(ICX),SY(ICY)) + + + SCORES(SX(ICX+LW),SY(ICY+LW)) +C CS(I) = CS(I) - SCORES(SX(CX(I)),CTONUM(SY(CY(I)))) +C + + SCORES(SX(CX(I)+LW),CTONUM(SY(CY(I)+LW))) +C WRITE(*,*)'I,CX(I),CY(I)',I,CX(I),CY(I) + CX(I) = CX(I) + 1 + CY(I) = CY(I) + 1 + 40 CONTINUE +C WRITE(*,*)J,CX(0),CY(0),(CS(K),K=-ND2,ND2) + IS = IMAXA(CS(-ND2),ND) + IF(IS.GE.MS) IDSCAN = IDSCAN + 1 +C WRITE(*,*)IS,IDSCAN +50 CONTINUE + DSCAN = REAL(IDSCAN)/NSTEPS + END + INTEGER FUNCTION IMAXA(I,N) + INTEGER I(N) + IMAXA = I(1) + DO 10 J=2,N + IMAXA = MAX(IMAXA,I(J)) + 10 CONTINUE + END + SUBROUTINE VPOUTR(IDEVO,IDEVN,NAME,LC,RC, + +SCORE,VNAME,TNAME,KCUT,LCUT,EMAX,IOK) + CHARACTER LINE*80,NAME*(*),VNAME*(*),TNAME*(*) + INTEGER RC + EXTERNAL NOTRL +C +C IDEVO original file +C IDEVN new reading file +C IDEVNG fofn for good data +C + 1005 FORMAT(A) +C +C Some ALU, so rename original file, and use old name for new file +C + TNAME = NAME + CLOSE(IDEVO) + K = INDEX(NAME,' ') + NAME(K:) = VNAME + IOK = NCFRS(TNAME,NAME) + IF(IOK.NE.0) THEN + WRITE(*,*)'Error renaming',TNAME + RETURN + END IF +C +C Reopen the file with its new name +C + CALL OPENRS(IDEVO,NAME,IOK,LRECL,2) + IF(IOK.NE.0) THEN + WRITE(*,*)'Error opening file',NAME + RETURN + END IF +C +C Open the new file with the original name +C + CALL OPENRS(IDEVN,TNAME,IOK,LRECL,1) + IF(IOK.NE.0) THEN + WRITE(*,*)'Error opening file',NAME + RETURN + END IF + 5 CONTINUE + READ(IDEVO,1005,ERR=6,END=7)LINE + IF(LINE(1:1).EQ.';') THEN + WRITE(IDEVN,1005)LINE(1:NOTRL(LINE,80,' ')) + GO TO 5 + END IF + 7 CONTINUE + WRITE(IDEVN,1000)LC,RC-LC+1,SCORE + IF(KCUT.GT.0) THEN + WRITE(IDEVN,1000)KCUT,LCUT-KCUT+1,EMAX + END IF + 1000 FORMAT(';;ALUS',2I7,F10.3) + WRITE(IDEVN,1005,ERR=6)LINE(1:NOTRL(LINE,80,' ')) + 8 CONTINUE + READ(IDEVO,1005,ERR=6,END=9)LINE + WRITE(IDEVN,1005,ERR=6)LINE(1:NOTRL(LINE,80,' ')) + GO TO 8 + 9 CONTINUE + CLOSE(IDEVO) + CLOSE(IDEVN) + IOK = 0 + RETURN + 6 CONTINUE + WRITE(*,*)'Error in VPOUTR' + IOK = 1 + END + INTEGER FUNCTION NCFRS(FROM,TO) + CHARACTER FROM*(*),TO*(*) + INTEGER RENAME + EXTERNAL RENAME +C +C rename file using system call to RENAME +C + IFD = 0 + ITD = 0 + IF(INDEX(FROM,'/').EQ.0) THEN + FROM = './'//FROM + IFD = 1 + END IF + IF(INDEX(TO,'/').EQ.0) THEN + TO = './'//TO + ITD = 1 + END IF + NCFRS = RENAME(FROM,TO) + IF(IFD.EQ.1) FROM = FROM(3:) + IF(ITD.EQ.1) TO = TO(3:) + END diff --git a/src/staden/sap.f b/src/staden/sap.f new file mode 100644 index 0000000..32fd7d8 --- /dev/null +++ b/src/staden/sap.f @@ -0,0 +1,622 @@ +C SAP (Sequence assembly program) +C AUTHOR RODGER STADEN +C 9-1-90 sent idev instead of kbout to join and enter +C 16-3-90 removed escape after bpause +C 2-5-90 Many changes related to introducing graphics +C 4-5-90 Allowed escape from getreg +C 9-5-90 Introduced a default gel reading +C 23-8-90 Added calls to SHOWFU +C 28-11-90 See dbsys89 +C 22-1-91 Changed use of showfi to include version number +C 27-1-91 Changed maxsav from 1000 to maxglm +C 13-04-91 removed initial CLEARV +C 28-7-91 added extra parameter for quality calc: mxgood, only the parts +C of reads upto this length are used +C 21-8-91 Added function to find internal joins +C 27-8-91 Added buffer flushing for database files + SUBROUTINE FMAIN() + INTEGER CHRSIZ,ECHRSZ + PARAMETER ( + + NAMLEN = 40, + + MAXDEV = 10, + + MAXSEQ=50000, + + MAXGLM=4096, + + MAXGL2=MAXGLM*2, + + MINMEN=-3, + + CHRSIZ=4, + + ECHRSZ = 6, + + IDT1=ECHRSZ*MAXGL2, + + IDT2=2*CHRSIZ*MAXGL2, + + IDT3=2*MAXGL2, + + IDT4=2*ECHRSZ*MAXGL2, + + MAXDB=1000) + INTEGER BOTOPT,TOPOPT,DEVNOS(MAXDEV) + INTEGER RELPG(MAXDB),LREG,RREG,ANS + INTEGER TEMP1(IDT1),TEMP2(IDT2),TEMP3(IDT4) + INTEGER LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER*(NAMLEN) HELPF,POINTF,FILNAM,NAMPRO,FILE,NAMARC,FILMAR + CHARACTER*(NAMLEN) SHONAM + CHARACTER GEL(MAXGLM) + CHARACTER GEL2(MAXGLM),GEL3(MAXGLM),GEL4(MAXGLM) + PARAMETER (BOTOPT=0,TOPOPT=35) + PARAMETER (HELPF='SAPHELP') + PARAMETER (POINTF='SAPHPNT') + PARAMETER (FILMAR='SAPMARG') + INTEGER HELPS(BOTOPT:TOPOPT),HELPE(BOTOPT:TOPOPT) + INTEGER MARGB(TOPOPT),MARGT(TOPOPT) + CHARACTER SEQ1(MAXSEQ) +C DBAUTO TEMP3A = TEMP1, SEQ3A = GEL3, SEQ2A = GEL2 +C MATCHA = GEL, SEQ4A = GEL4 + PARAMETER (LENGTH = 6, + + MAXCON = 500, + + MAXSAV = MAXGLM, + + LPOWRC = CHRSIZ**LENGTH) + INTEGER CONST(LENGTH) + INTEGER POSNS(MAXSEQ),WORDP(LPOWRC),WORDN(LPOWRC),GELN(MAXGLM) + INTEGER CENDS(MAXCON),NENDS(MAXCON) + CHARACTER SEQC2(MAXGLM,2),SEQG2(MAXGLM,2) + CHARACTER SEQ5(MAXGLM),SEQG3(MAXGLM),SEQC3(MAXGLM) + INTEGER SAV1(MAXSAV),SAV2(MAXSAV),SAV3(MAXSAV) + INTEGER WINDOW +C DBAUTO + EQUIVALENCE (TEMP2,TEMP1) + EQUIVALENCE (TEMP1,TEMP3) + IDM = 5 + CALL INITS + CALL INITLU(IDM) + CALL INTHLP('sap', TOPOPT) + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + CALL OPENGR(DEVNOS(10)) + WRITE(KBOUT,1000) +1000 FORMAT( + +' SAP (Sequence assembly program) V5.0 Aug 1991',/, + +' Author: Rodger Staden') + IGORT = 0 + IDEV = KBOUT +C GET SCREEN AND MARGIN SIZES + CALL GETMRG(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + +TOPOPT,DEVNOS(5),FILMAR) + CALL SETHLP(HELPS,HELPE,BOTOPT,TOPOPT,POINTF,DEVNOS(5),KBOUT) + CALL INITGR(KBIN,KBOUT,HELPS(0),HELPE(0),HELPF,IDEVH) + IOPEN=1 + LINLEN=50 + PERCD=0.75 + WINDOW = 25 + MXGOOD = MAXGLM + IGWIND = 1000 + FILE = ' ' + MAXGEL = MAXGLM + SHONAM = ' ' + CALL SHOWFI(KBOUT,SHONAM) + ANS = 0 + CALL YESONO(ANS,'Open existing database','Start new database', + + HELPS(3),HELPE(3),HELPF,DEVNOS(5),KBIN,KBOUT) + IF(ANS.LT.0)GO TO 10 + IF(ANS.GT.1)GO TO 10 + MAXGEL = MAXGLM + IF(IOPEN.EQ.0)THEN + CLOSE(UNIT=DEVNOS(1)) + CLOSE(UNIT=DEVNOS(2)) + CLOSE(UNIT=DEVNOS(3)) + IOPEN=1 + END IF + IDBSIZ=MAXDB + IDBSIS=IDBSIZ + IF(ANS.EQ.1)THEN +C CALL SHOWFU(KBOUT,'Start new database') + CALL DBSTAR(NAMPRO,GEL,IDBSIS,IDBSIZ,KBIN,KBOUT,DEVNOS(1), + + DEVNOS(2),DEVNOS(3),IERR,HELPS(NOPT),HELPE(NOPT), + + DEVNOS(5),HELPF,MAXGEL,MAXGLM,IDM) + NGELS=0 + NCONTS=0 + LLINO = 0 + IF(IERR.EQ.0) THEN + IOPEN=0 + SHONAM = NAMPRO(1:MAX(1,INDEX(NAMPRO,'.'))+1) + CALL SHOWFI(KBOUT,SHONAM) + END IF + CALL INITLU(IDM) + GO TO 10 + END IF + IF(ANS.EQ.0)THEN +C CALL SHOWFU(KBOUT,'Open database') + NGELS=0 + NCONTS=0 + CALL DBOPEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,NAMPRO,GEL, + + IDBSIS,IDBSIZ,JERR,KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + + MAXGEL,MAXGLM,LLINO,IDM, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(JERR.EQ.0)IOPEN=0 + IF(NGELS.GT.0)CALL DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,KBOUT) + CALL INITLU(IDM) + SHONAM = NAMPRO(1:MAX(1,INDEX(NAMPRO,'.'))+1) + CALL SHOWFI(KBOUT,SHONAM) + GO TO 10 + END IF + MENUN = 0 +10 CONTINUE +C +C Flush the database buffers, just in case! +C + CALL FLUSHL(DEVNOS(1)) + CALL FLUSHL(DEVNOS(2)) + CALL FLUSHL(DEVNOS(3)) + CALL BPAUSE(KBIN,KBOUT,IOK) +C CALL SHOWFU(KBOUT,' ') +C IF(IOK.NE.0) GO TO 9999 +C CALL DBMENT(MENU,NOPT,TOPOPT,HELPS(0),HELPE(0),HELPF,DEVNOS(5), +C +KBIN,KBOUT) + CALL MENU(NOPT, KOPT, MENUN, TOPOPT, MINMEN, KBIN, KBOUT, + + HELPS(0), HELPE(0), HELPF, DEVNOS(5)) + IF(NOPT.EQ.3)THEN + ANS = 0 + CALL YESONO(ANS,'Open existing database','Start new database', + + HELPS(3),HELPE(3),HELPF,DEVNOS(5),KBIN,KBOUT) + IF(ANS.LT.0)GO TO 10 + IF(ANS.GT.1)GO TO 10 + MAXGEL = MAXGLM + IF(IOPEN.EQ.0)THEN + CLOSE(UNIT=DEVNOS(1)) + CLOSE(UNIT=DEVNOS(2)) + CLOSE(UNIT=DEVNOS(3)) + IOPEN=1 + END IF + IDBSIZ=MAXDB + IDBSIS=IDBSIZ + IF(ANS.EQ.1)THEN +C CALL SHOWFU(KBOUT,'Start a database') + CALL DBSTAR(NAMPRO,GEL,IDBSIS,IDBSIZ,KBIN,KBOUT,DEVNOS(1), + + DEVNOS(2),DEVNOS(3),IERR,HELPS(NOPT),HELPE(NOPT), + + DEVNOS(5),HELPF,MAXGEL,MAXGLM,IDM) + NGELS=0 + NCONTS=0 + IF(IERR.EQ.0)IOPEN=0 + CALL INITLU(IDM) + SHONAM = NAMPRO(1:MAX(1,INDEX(NAMPRO,'.'))+1) + CALL SHOWFI(KBOUT,SHONAM) + GO TO 10 + END IF + IF(ANS.EQ.0)THEN +C CALL SHOWFU(KBOUT,'Open database') + NGELS=0 + NCONTS=0 + CALL DBOPEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,NAMPRO,GEL, + + IDBSIS,IDBSIZ,JERR,KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + + MAXGEL,MAXGLM,LLINO,IDM, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(JERR.EQ.0)IOPEN=0 + IF(NGELS.GT.0)CALL DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,KBOUT) + CALL INITLU(IDM) + SHONAM = NAMPRO(1:MAX(1,INDEX(NAMPRO,'.'))+1) + CALL SHOWFI(KBOUT,SHONAM) + GO TO 10 + END IF + END IF +C STOP + IF(NOPT.EQ.2)THEN + IF(NGELS.GT.0)CALL DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,KBOUT) + GO TO 9999 + END IF +C clear all +C + IF(NOPT.EQ.10)THEN +C CALL SHOWFU(KBOUT,'Clear graphics') + CALL CLEARG + GO TO 10 + END IF +C clear VT100 +C + IF(NOPT.EQ.11)THEN +C CALL SHOWFU(KBOUT,'Clear text') + CALL CLEARV + GO TO 10 + END IF +C +C xhairs +C + IF(NOPT.EQ.13) THEN +C CALL SHOWFU(KBOUT,'Use xhair') + CALL XHSAP(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,NCONTS,LLINO,LINCON,LREG,RREG, + + WINDOW,IGWIND,LENCON,IDEPTH,JDEPTH, + + MARGL,MARGR,MARGB,MARGT,TOPOPT,ISXMAX,ISYMAX,KBIN,IDEV, + + KBOUT,GEL,GEL2,DEVNOS(2),DEVNOS(3),LINLEN,PERCD,MAXGEL,IDM, + + SEQ1,MAXSEQ,NGELS,TEMP3,ECHRSZ,MAXGL2,GEL3,GEL4,33,34,29, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MXGOOD) + GO TO 10 + END IF +C +C CHANGE MARGINS +C + IF(NOPT.EQ.14)THEN +C CALL SHOWFU(KBOUT,'Change margins') + CALL MARGC(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + + HELPS(NOPT),HELPE(NOPT),TOPOPT,HELPF,DEVNOS(5),KBIN,KBOUT) + GO TO 10 + END IF +C ruler +C + IF(NOPT.EQ.12) THEN + LENCON = RREG - LREG + 1 + IF(LENCON.GT.0) THEN +C CALL SHOWFU(KBOUT,'Show ruler') + CALL RULER(LREG,RREG,MARGL,MARGR, + + MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX,KBIN,KBOUT,1, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + GO TO 10 + END IF + END IF +C labler + IF(NOPT.EQ.15)THEN +C CALL SHOWFU(KBOUT,'Label diagram') + CALL LABLER(KBIN,KBOUT,ISXMAX,ISYMAX, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + GO TO 10 + END IF + IF(NOPT.EQ.16) THEN +C CALL SHOWFU(KBOUT,'Plot map') +C map + LENCON = RREG - LREG + 1 + IF(LENCON.GT.0) THEN + CALL PLTMAP(DEVNOS(4),FILNAM,LENCON,MARGL,MARGR,MARGB(NOPT), + + MARGT(NOPT),ISXMAX,ISYMAX,LREG,RREG,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + END IF + GO TO 10 + END IF + IF((NOPT.EQ.21).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Enter new gel reading') +C ENTER + CALL ENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + GEL,GEL2,IDBSIZ,IDEV,KBIN,KBOUT,DEVNOS(1),DEVNOS(2), + + DEVNOS(3),DEVNOS(4),LINLEN,PERCD,FILNAM, + + HELPS(NOPT),HELPE(NOPT),HELPS(4),HELPE(4),HELPF,DEVNOS(5), + + MAXGEL,IDM,LLINO) + GO TO 10 + END IF + IF((NOPT.EQ.25).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Show relationships') +C PRINT DB + CALL DBPRNT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ,IDEV, + + KBIN,KBOUT,DEVNOS(3),LLINO, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + GO TO 10 + END IF + IF((NOPT.EQ.5).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Display contig') +C DISPLAY + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + + LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + 1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IERR) + IF(IERR.EQ.0)THEN + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + + GEL,LLINO,LINCON,LREG,RREG,GEL2,I1,I2,0,I,IDBSIZ,IDEV,KBOUT, + + DEVNOS(2),DEVNOS(3),LINLEN,PERCD,MAXGEL,IDM) + END IF + END IF + GO TO 10 + END IF +200 CONTINUE + IF((NOPT.EQ.22).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Join contigs') +C JOIN + WRITE(KBOUT,1006) +1006 FORMAT(' Which contigs do you want to join ?') + WRITE(KBOUT,1017) +1017 FORMAT( ' Left contig') + LLINOL = 0 + LLINOR = 0 + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LNCONL,LLINOL, + + IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + WRITE(KBOUT,1018) +1018 FORMAT( ' Right contig') + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + LNCONR,LLINOR,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN +C CHECK TO SEE IF MAKING A CIRCLE! + IF(LLINOL.EQ.LLINOR)THEN + WRITE(KBOUT,*)' Making circles is not allowed!' + GO TO 200 + END IF + CALL JOIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + LNCONL,LLINOL,LNCONR,LLINOR,GEL,GEL2, + + IDBSIZ,IDEV,KBIN,KBOUT,DEVNOS(1),DEVNOS(2), + + DEVNOS(3),LINLEN,PERCD, + + HELPS(NOPT),HELPE(NOPT),HELPS(4),HELPE(4),HELPF,DEVNOS(5), + + MAXGEL,IDM) + END IF + END IF + GO TO 10 + END IF + IF((NOPT.EQ.23).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Complement contig') +C COMPLEMENT + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + LINCON,LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL CMPLMT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + LINCON,LLINO,GEL,IDBSIZ,KBOUT,DEVNOS(1),DEVNOS(2), + + MAXGEL) + END IF + GO TO 10 + END IF +C EDIT + IF((NOPT.EQ.4).AND.(IOPEN.EQ.0))THEN +C EDIT DB + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + + LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL DBEDIT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + GEL,LINCON,0,0,IDBSIZ,KBIN,KBOUT,DEVNOS(1),DEVNOS(2), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MAXGEL) + END IF + GO TO 10 + END IF +C PLOT SINGLE CONTIG + IF((NOPT.EQ.33).AND.(IOPEN.EQ.0))THEN + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + + LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + 1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IERR) + IF(IERR.EQ.0)THEN + LENCON = RREG - LREG + 1 + CALL FDEPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX) + END IF + END IF + GO TO 10 + END IF + IF((NOPT.EQ.26).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Alter relationships') +C FIX + CALL DBFIX(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + GEL,GEL2,IDBSIZ,KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT), + + HELPS(4),HELPE(4),HELPF,DEVNOS(5),MAXGEL) + GO TO 10 + END IF + IF((NOPT.EQ.24).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Copy database') +C COPY + CALL DBCOPY(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,GEL, + + NAMPRO,DEVNOS(4),IDBSIZ,JERR,KBIN,KBOUT,DEVNOS(1), + + DEVNOS(2),DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MAXGEL,MAXDB,IDM) + GO TO 10 + END IF + IF((NOPT.EQ.19).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Check database for consistency') +C CHECK + CALL DBCHEK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + TEMP1,IERR,KBOUT) + GO TO 10 + END IF + IF((NOPT.EQ.29).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Examine quality') +C SCAN + IDIM1=MAXSEQ + CALL DBSCAN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1, + + IDIM1,GEL,IDBSIZ,TEMP3,2,ECHRSZ,MAXGL2, + + KBIN,KBOUT,DEVNOS(2),IDEV,LINLEN,PERCD, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MAXGEL,GEL3,GEL4, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX,DEVNOS(3), + + LLINO,LINCON,LREG,RREG,MXGOOD) + GO TO 10 + END IF + IF((NOPT.EQ.8).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Make consensus') +C CONSENSUS + IDIM1=MAXSEQ + CALL CONSEN(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS,SEQ1, + + IDIM1,GEL,IDBSIZ,TEMP1,ECHRSZ,MAXGL2, + + KBIN,KBOUT,DEVNOS(2),DEVNOS(4),FILNAM, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MAXGEL,IDM,PERCD, + + DEVNOS(3),LLINO) + CLOSE(UNIT=DEVNOS(4)) + GO TO 10 + END IF + IF(NOPT.EQ.7)THEN +C CALL SHOWFU(KBOUT,'Redirect output') + CALL REDIR(IDEV,DEVNOS(6),DEVNOS(10),IGORT,FILNAM,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),KOPT) + GO TO 10 + END IF +C HELP + IF(NOPT.EQ.1)THEN +C CALL SHOWFU(KBOUT,'Help') +C HELP + CALL HELP(HELPS,HELPE,BOTOPT,TOPOPT, + + HELPF,DEVNOS(5),KBIN,KBOUT) + GO TO 10 + END IF + IF(NOPT.EQ.27)THEN +C CALL SHOWFU(KBOUT,'Set parameters') +C SET DISPLAY PARAMETERS + MN = 10 + MX = 100 + CALL GETINT(MN,MX,LINLEN,'Display line length', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) LINLEN = IVAL + IPCMIN = 51 + IPCMAX = 100 + IPCD = INT(PERCD*100.) + CALL GETINT(IPCMIN,IPCMAX,IPCD, + + 'Percentage score for consensus', + + IPVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) THEN + PERCD = REAL(IPVAL)/100. + END IF + MN = 1 + MX = MAXGLM + CALL GETINT(MN,MX,MXGOOD, + + 'Maximum read length for quality analysis', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) MXGOOD = IVAL + MN = 1 + MX = MAXSEQ + CALL GETINT(MN,MX,WINDOW,'Text window length', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) WINDOW = IVAL + MN = 1 + MX = MAXSEQ + CALL GETINT(MN,MX,IGWIND,'Graphics window length', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) IGWIND = IVAL + GO TO 10 + END IF + IF(NOPT.EQ.6)THEN +C CALL SHOWFU(KBOUT,'Display text file') + CALL TTEXT(DEVNOS(4),FILNAM,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + CLOSE(UNIT=DEVNOS(4)) + GO TO 10 + END IF + IF(NOPT.EQ.28) THEN +C CALL SHOWFU(KBOUT,'Highlight disagreements') + CALL HIGHLT(SEQ1,SEQ1(6001),SEQ1(10001),KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),DEVNOS(7), + + DEVNOS(8),FILE,IOK) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + GO TO 10 + END IF + IF((NOPT.EQ.34).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'Plot contigs') +C PLOT ALL CONTIGS + CALL PLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,0,0, + + NCONTS,MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX) + GO TO 10 + END IF +C AUTO EDIT + IF((NOPT.EQ.30).AND.(IOPEN.EQ.0))THEN +C CALL SHOWFU(KBOUT,'AutoEdit a contig') + WRITE(KBOUT,*)'Make a copy first!' + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + + LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + 1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IERR) + IF(IERR.EQ.0) THEN + IDIM1=MAXSEQ + CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1,IDIM1, + + GEL,LREG,RREG,LLINO,IDBSIZ,TEMP1,ECHRSZ,MAXGL2, + + DEVNOS(2),MAXGEL,IDM,PERCD) + CALL AEDIT(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LLINO,LINCON, + + GEL,MAXGEL,SEQ1,IDIM1,DEVNOS(2),DEVNOS(1),LREG,RREG, + + KBOUT) + END IF + END IF + GO TO 10 + END IF + IF((NOPT.EQ.9).AND.(IOPEN.EQ.0)) THEN +C CALL SHOWFU(KBOUT,'Screen Edit a contig') + CALL CEDT(KBIN,KBOUT, + + POSNS(1),POSNS(1001),POSNS(2001), + + MAXDB,POSNS(3001),POSNS(3101),50, + + RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,NGELS,NCONTS,GEL,GEL2,MAXGEL,LINCON,PERCD,IDM, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),DEVNOS(4), + + DEVNOS(1),DEVNOS(2),DEVNOS(3),LINLEN,FILNAM,LLINO,IOK) + GO TO 10 + END IF + IF(NOPT.EQ.17) THEN +C CALL SHOWFU(KBOUT,'Screen for restriction sites') + CALL SCRENR(GEL2,MAXGEL,GEL3,NAMARC,FILE, + + DEVNOS(4),DEVNOS(7),DEVNOS(8),DEVNOS(9),IDEV,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + CLOSE(UNIT=DEVNOS(4)) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + CLOSE(UNIT=DEVNOS(9)) + GO TO 10 + END IF + IF(NOPT.EQ.18) THEN +C CALL SHOWFU(KBOUT,'Screen against vector') + CALL SCRENV(MAXGEL,WORDP,WORDN,LPOWRC,POSNS,GELN, + + SEQ1,MAXSEQ,GEL2,GEL3,GEL4,LENGTH, + + SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON,CONST, + + KBIN,KBOUT,DEVNOS(4),DEVNOS(7),DEVNOS(8),DEVNOS(9),IDEV, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),NAMARC,FILE,IOK) + CLOSE(UNIT=DEVNOS(4)) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + CLOSE(UNIT=DEVNOS(9)) + GO TO 10 + END IF + IF((NOPT.EQ.20).AND.(IOPEN.EQ.0)) THEN +C CALL SHOWFU(KBOUT,'Auto assemble sequences') + CALL DBAUTO(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ, + +NGELS,NCONTS,MAXGEL, + +TEMP1,WORDP,WORDN,LPOWRC,POSNS,GELN, + +SEQ1,MAXSEQ,GEL2,GEL3,GEL4,SEQ5,SEQC2,SEQG2,GEL, + +MAXGLM,MAXGL2,CHRSIZ,ECHRSZ,LENGTH, + +SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON,CONST, + +KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + +DEVNOS(4),DEVNOS(7),DEVNOS(8),IDEV, + +HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),NAMARC,NAMPRO,FILE, + +PERCD,IOPEN,IDM,SEQG3,SEQC3,IOK) + CLOSE(UNIT=DEVNOS(4)) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + GO TO 10 + END IF + IF((NOPT.EQ.35).AND.(IOPEN.EQ.0).AND.(IDM.EQ.5)) THEN +C CALL SHOWFU(KBOUT,'Find internal joins') + CALL AUTOJ(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ, + +NGELS,NCONTS,MAXGEL, + +TEMP1,WORDP,WORDN,LPOWRC,POSNS,GELN, + +SEQ1,MAXSEQ,GEL2,GEL3,GEL4,SEQ5,SEQC2,SEQG2,GEL, + +MAXGLM,MAXGL2,CHRSIZ,ECHRSZ,LENGTH, + +SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON,CONST, + +KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + +DEVNOS(4),DEVNOS(7),DEVNOS(8),IDEV, + +HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),NAMARC,NAMPRO,FILE, + +PERCD,IOPEN,IDM,SEQG3,SEQC3,IOK) + CLOSE(UNIT=DEVNOS(4)) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + GO TO 10 + END IF + IF((NOPT.EQ.32).AND.(IOPEN.EQ.0)) THEN +C CALL SHOWFU(KBOUT,'Extract gel readings') + CALL GELOUT(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ,NGELS, + + NCONTS,GEL,MAXGEL,DEVNOS(2),DEVNOS(3),DEVNOS(4),DEVNOS(7), + + KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),FILE) + CLOSE(UNIT=DEVNOS(4)) + GO TO 10 + END IF + IF(NOPT.EQ.31) THEN +C CALL SHOWFU(KBOUT,'Type in gel readings') + CALL BATIN(SEQ1,MAXSEQ,KBIN,KBOUT, + + DEVNOS(4),DEVNOS(7),IDEV, + + FILNAM,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + GO TO 10 + END IF + IF((NOPT.GT.3).AND.(IOPEN.NE.0))THEN + WRITE(KBOUT,1012) +1012 FORMAT(' You have not opened a database!') + GO TO 10 + END IF + GO TO 10 +9999 CONTINUE + CALL SHUTD + END diff --git a/src/staden/sapf.f b/src/staden/sapf.f new file mode 100644 index 0000000..1f2885d --- /dev/null +++ b/src/staden/sapf.f @@ -0,0 +1,775 @@ +C SAPF (Sequence assembly program) +C AUTHOR RODGER STADEN +C 9-1-90 sent idev instead of kbout to join and enter +C 16-3-90 removed escape after bpause +C 2-5-90 Many changes related to introducing graphics +C 4-5-90 Allowed escape from getreg +C 9-5-90 Introduced a default gel reading +C 23-8-90 Added calls to SHOWFU +C 28-11-90 See dbsys89 +C 22-1-91 Changed use of showfi to include version number + SUBROUTINE FMAIN() + INTEGER CHRSIZ,ECHRSZ + PARAMETER ( + + NAMLEN = 40, + + MAXDEV = 10, + + MAXSEQ=50000, + + MAXGLM=4096, + + MAXGL2=MAXGLM*2, + + CHRSIZ=4, + + ECHRSZ = 6, + + IDT1=ECHRSZ*MAXGL2, + + IDT2=2*CHRSIZ*MAXGL2, + + IDT3=2*MAXGL2, + + IDT4=2*ECHRSZ*MAXGL2, + + MAXDB=1000) + INTEGER BOTOPT,TOPOPT,DEVNOS(MAXDEV) + INTEGER RELPG(MAXDB),LREG,RREG,ANS + INTEGER TEMP1(IDT1),TEMP2(IDT2),TEMP3(IDT4) + INTEGER LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER*(NAMLEN) HELPF,POINTF,FILNAM,NAMPRO,FILE,NAMARC,FILMAR + CHARACTER*(NAMLEN) SHONAM + CHARACTER GEL(MAXGLM) + CHARACTER GEL2(MAXGLM),GEL3(MAXGLM),GEL4(MAXGLM) + PARAMETER (BOTOPT=0,TOPOPT=34) + PARAMETER (HELPF='SAPHELP') + PARAMETER (POINTF='SAPHPNT') + PARAMETER (FILMAR='SAPMARG') + INTEGER HELPS(BOTOPT:TOPOPT),HELPE(BOTOPT:TOPOPT) + INTEGER MARGB(TOPOPT),MARGT(TOPOPT) + CHARACTER SEQ1(MAXSEQ) +C DBAUTO TEMP3A = TEMP1, SEQ3A = GEL3, SEQ2A = GEL2 +C MATCHA = GEL, SEQ4A = GEL4 + PARAMETER (LENGTH = 6, + + MAXCON = 500, + + MAXSAV = 1000, + + LPOWRC = CHRSIZ**LENGTH) + INTEGER CONST(LENGTH) + INTEGER POSNS(MAXSEQ),WORDP(LPOWRC),WORDN(LPOWRC),GELN(MAXGLM) + INTEGER CENDS(MAXCON),NENDS(MAXCON) + CHARACTER SEQC2(MAXGLM,2),SEQG2(MAXGLM,2) + CHARACTER SEQ5(MAXGLM),SEQG3(MAXGLM),SEQC3(MAXGLM) + INTEGER SAV1(MAXSAV),SAV2(MAXSAV),SAV3(MAXSAV) + INTEGER WINDOW +C DBAUTO + EQUIVALENCE (TEMP2,TEMP1) + EQUIVALENCE (TEMP1,TEMP3) + IDM = 5 + CALL INITS + CALL INITLU(IDM) + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + CALL OPENGR(DEVNOS(10)) + CALL CLEARV + WRITE(KBOUT,1000) +1000 FORMAT(/, + +' SAPF (Sequence assembly program (flat files) V3.1 Jan 1991',/, + +' Author: Rodger Staden') + IGORT = 0 + IDEV = KBOUT +C GET SCREEN AND MARGIN SIZES + CALL GETMRG(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + +TOPOPT,DEVNOS(5),FILMAR) + CALL SETHLP(HELPS,HELPE,BOTOPT,TOPOPT,POINTF,DEVNOS(5),KBOUT) + CALL INITGR(KBIN,KBOUT,HELPS(0),HELPE(0),HELPF,IDEVH) + IOPEN=1 + LINLEN=50 + PERCD=0.75 + WINDOW = 25 + IGWIND = 1000 + FILE = ' ' + MAXGEL = MAXGLM + SHONAM = ' ' + CALL SHOWFI(KBOUT,SHONAM) + ANS = 0 + CALL YESONO(ANS,'Open existing database','Start new database', + + HELPS(3),HELPE(3),HELPF,DEVNOS(5),KBIN,KBOUT) + IF(ANS.LT.0)GO TO 10 + IF(ANS.GT.1)GO TO 10 + MAXGEL = MAXGLM + IF(IOPEN.EQ.0)THEN + CLOSE(UNIT=DEVNOS(1)) + CLOSE(UNIT=DEVNOS(2)) + CLOSE(UNIT=DEVNOS(3)) + IOPEN=1 + END IF + IDBSIZ=MAXDB + IDBSIS=IDBSIZ + IF(ANS.EQ.1)THEN + CALL SHOWFU(KBOUT,'Start new database from flat file') + CALL DBSTAF(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,NAMPRO,GEL, + + IDBSIS,IDBSIZ,JERR,KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + + MAXGEL,MAXGLM,LLINO,IDM, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(JERR.EQ.0)IOPEN=0 + IF(NGELS.GT.0)CALL DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,KBOUT) + CALL INITLU(IDM) + SHONAM = NAMPRO(1:MAX(1,INDEX(NAMPRO,'.'))+1) + CALL SHOWFI(KBOUT,SHONAM) + GO TO 10 + END IF + IF(ANS.EQ.0)THEN + CALL SHOWFU(KBOUT,'Open database') + NGELS=0 + NCONTS=0 + CALL DBOPEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,NAMPRO,GEL, + + IDBSIS,IDBSIZ,JERR,KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + + MAXGEL,MAXGLM,LLINO,IDM, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(JERR.EQ.0)IOPEN=0 + IF(NGELS.GT.0)CALL DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,KBOUT) + CALL INITLU(IDM) + SHONAM = NAMPRO(1:MAX(1,INDEX(NAMPRO,'.'))+1) + CALL SHOWFI(KBOUT,SHONAM) + GO TO 10 + END IF + MENU = 0 +10 CONTINUE + CALL BPAUSE(KBIN,KBOUT,IOK) + CALL SHOWFU(KBOUT,' ') +C IF(IOK.NE.0) GO TO 9999 + CALL DBMENT(MENU,NOPT,TOPOPT,HELPS(0),HELPE(0),HELPF,DEVNOS(5), + +KBIN,KBOUT) + IF(NOPT.EQ.3)THEN + ANS = 0 + CALL YESONO(ANS,'Open existing database','Start new database', + + HELPS(3),HELPE(3),HELPF,DEVNOS(5),KBIN,KBOUT) + IF(ANS.LT.0)GO TO 10 + IF(ANS.GT.1)GO TO 10 + MAXGEL = MAXGLM + IF(IOPEN.EQ.0)THEN + CLOSE(UNIT=DEVNOS(1)) + CLOSE(UNIT=DEVNOS(2)) + CLOSE(UNIT=DEVNOS(3)) + IOPEN=1 + END IF + IDBSIZ=MAXDB + IDBSIS=IDBSIZ + IF(ANS.EQ.1)THEN + CALL SHOWFU(KBOUT,'Start a database') + CALL DBSTAR(NAMPRO,GEL,IDBSIS,IDBSIZ,KBIN,KBOUT,DEVNOS(1), + + DEVNOS(2),DEVNOS(3),IERR,HELPS(NOPT),HELPE(NOPT), + + DEVNOS(5),HELPF,MAXGEL,MAXGLM,IDM) + NGELS=0 + NCONTS=0 + IF(IERR.EQ.0)IOPEN=0 + CALL INITLU(IDM) + SHONAM = NAMPRO(1:MAX(1,INDEX(NAMPRO,'.'))+1) + CALL SHOWFI(KBOUT,SHONAM) + GO TO 10 + END IF + IF(ANS.EQ.0)THEN + CALL SHOWFU(KBOUT,'Open database') + NGELS=0 + NCONTS=0 + CALL DBOPEN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,NAMPRO,GEL, + + IDBSIS,IDBSIZ,JERR,KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + + MAXGEL,MAXGLM,LLINO,IDM, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(JERR.EQ.0)IOPEN=0 + IF(NGELS.GT.0)CALL DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS, + + NCONTS,IDBSIZ,KBOUT) + CALL INITLU(IDM) + SHONAM = NAMPRO(1:MAX(1,INDEX(NAMPRO,'.'))+1) + CALL SHOWFI(KBOUT,SHONAM) + GO TO 10 + END IF + END IF +C STOP + IF(NOPT.EQ.2)THEN + IF(NGELS.GT.0)CALL DBSTAT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + IDBSIZ,KBOUT) + GO TO 9999 + END IF +C clear all +C + IF(NOPT.EQ.10)THEN + CALL SHOWFU(KBOUT,'Clear graphics') + CALL CLEARG + GO TO 10 + END IF +C clear VT100 +C + IF(NOPT.EQ.11)THEN + CALL SHOWFU(KBOUT,'Clear text') + CALL CLEARV + GO TO 10 + END IF +C +C xhairs +C + IF(NOPT.EQ.13) THEN + CALL SHOWFU(KBOUT,'Use xhair') + CALL XHSAP(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,NCONTS,LLINO,LINCON,LREG,RREG, + + WINDOW,IGWIND,LENCON,IDEPTH,JDEPTH, + + MARGL,MARGR,MARGB,MARGT,TOPOPT,ISXMAX,ISYMAX,KBIN,IDEV, + + KBOUT,GEL,GEL2,DEVNOS(2),DEVNOS(3),LINLEN,PERCD,MAXGEL,IDM, + + SEQ1,MAXSEQ,NGELS,TEMP3,ECHRSZ,MAXGL2,GEL3,GEL4,33,34,29, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + GO TO 10 + END IF +C +C CHANGE MARGINS +C + IF(NOPT.EQ.14)THEN + CALL SHOWFU(KBOUT,'Change margins') + CALL MARGC(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + + HELPS(NOPT),HELPE(NOPT),TOPOPT,HELPF,DEVNOS(5),KBIN,KBOUT) + GO TO 10 + END IF +C ruler +C + IF(NOPT.EQ.12) THEN + LENCON = RREG - LREG + 1 + IF(LENCON.GT.0) THEN + CALL SHOWFU(KBOUT,'Show ruler') + CALL RULER(LREG,RREG,MARGL,MARGR, + + MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX,KBIN,KBOUT,1, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + GO TO 10 + END IF + END IF +C labler + IF(NOPT.EQ.15)THEN + CALL SHOWFU(KBOUT,'Label diagram') + CALL LABLER(KBIN,KBOUT,ISXMAX,ISYMAX, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + GO TO 10 + END IF + IF(NOPT.EQ.16) THEN + CALL SHOWFU(KBOUT,'Plot map') +C map + LENCON = RREG - LREG + 1 + IF(LENCON.GT.0) THEN + CALL PLTMAP(DEVNOS(4),FILNAM,LENCON,MARGL,MARGR,MARGB(NOPT), + + MARGT(NOPT),ISXMAX,ISYMAX,LREG,RREG,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + END IF + GO TO 10 + END IF + IF((NOPT.EQ.21).AND.(IOPEN.EQ.0))THEN + CALL SHOWFU(KBOUT,'Enter new gel reading') +C ENTER + CALL ENTER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + GEL,GEL2,IDBSIZ,IDEV,KBIN,KBOUT,DEVNOS(1),DEVNOS(2), + + DEVNOS(3),DEVNOS(4),LINLEN,PERCD,FILNAM, + + HELPS(NOPT),HELPE(NOPT),HELPS(4),HELPE(4),HELPF,DEVNOS(5), + + MAXGEL,IDM,LLINO) + GO TO 10 + END IF + IF((NOPT.EQ.25).AND.(IOPEN.EQ.0))THEN + CALL SHOWFU(KBOUT,'Show relationships') +C PRINT DB + CALL DBPRNT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ,IDEV, + + KBIN,KBOUT,DEVNOS(3),LLINO, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + GO TO 10 + END IF + IF((NOPT.EQ.5).AND.(IOPEN.EQ.0))THEN + CALL SHOWFU(KBOUT,'Display contig') +C DISPLAY + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + + LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + 1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IERR) + IF(IERR.EQ.0)THEN + CALL DSPLAY(RELPG,LNGTHG,LNBR,RNBR, + + GEL,LLINO,LINCON,LREG,RREG,GEL2,I1,I2,0,I,IDBSIZ,IDEV,KBOUT, + + DEVNOS(2),DEVNOS(3),LINLEN,PERCD,MAXGEL,IDM) + END IF + END IF + GO TO 10 + END IF +200 CONTINUE + IF((NOPT.EQ.22).AND.(IOPEN.EQ.0))THEN + CALL SHOWFU(KBOUT,'Join contigs') +C JOIN + WRITE(KBOUT,1006) +1006 FORMAT(' Which contigs do you want to join ?') + WRITE(KBOUT,1017) +1017 FORMAT( ' Left contig') + LLINOL = 0 + LLINOR = 0 + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LNCONL,LLINOL, + + IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + WRITE(KBOUT,1018) +1018 FORMAT( ' Right contig') + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + LNCONR,LLINOR,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN +C CHECK TO SEE IF MAKING A CIRCLE! + IF(LLINOL.EQ.LLINOR)THEN + WRITE(KBOUT,*)' Making circles is not allowed!' + GO TO 200 + END IF + CALL JOIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + LNCONL,LLINOL,LNCONR,LLINOR,GEL,GEL2, + + IDBSIZ,IDEV,KBIN,KBOUT,DEVNOS(1),DEVNOS(2), + + DEVNOS(3),LINLEN,PERCD, + + HELPS(NOPT),HELPE(NOPT),HELPS(4),HELPE(4),HELPF,DEVNOS(5), + + MAXGEL,IDM) + END IF + END IF + GO TO 10 + END IF + IF((NOPT.EQ.23).AND.(IOPEN.EQ.0))THEN + CALL SHOWFU(KBOUT,'Complement contig') +C COMPLEMENT + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + LINCON,LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL CMPLMT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + LINCON,LLINO,GEL,IDBSIZ,KBOUT,DEVNOS(1),DEVNOS(2), + + MAXGEL) + END IF + GO TO 10 + END IF +C EDIT + IF((NOPT.EQ.4).AND.(IOPEN.EQ.0))THEN +C EDIT DB + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + + LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL DBEDIT(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + GEL,LINCON,0,0,IDBSIZ,KBIN,KBOUT,DEVNOS(1),DEVNOS(2), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MAXGEL) + END IF + GO TO 10 + END IF +C PLOT SINGLE CONTIG + IF((NOPT.EQ.33).AND.(IOPEN.EQ.0))THEN + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + + LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + 1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IERR) + IF(IERR.EQ.0)THEN + LENCON = RREG - LREG + 1 + CALL FDEPTH(RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,LLINO,LREG,RREG,LENCON, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX) + END IF + END IF + GO TO 10 + END IF + IF((NOPT.EQ.26).AND.(IOPEN.EQ.0))THEN + CALL SHOWFU(KBOUT,'Alter relationships') +C FIX + CALL DBFIX(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + GEL,GEL2,IDBSIZ,KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT), + + HELPS(4),HELPE(4),HELPF,DEVNOS(5),MAXGEL) + GO TO 10 + END IF + IF((NOPT.EQ.24).AND.(IOPEN.EQ.0))THEN + CALL SHOWFU(KBOUT,'Copy database to flat format') +C COPY + CALL DBCOPF(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,GEL, + + NAMPRO,DEVNOS(4),IDBSIZ,JERR,KBIN,KBOUT,DEVNOS(1), + + DEVNOS(2),DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MAXGEL,MAXDB,IDM) + GO TO 10 + END IF + IF((NOPT.EQ.19).AND.(IOPEN.EQ.0))THEN + CALL SHOWFU(KBOUT,'Check database for consistency') +C CHECK + CALL DBCHEK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + TEMP1,IERR,KBOUT) + GO TO 10 + END IF + IF((NOPT.EQ.29).AND.(IOPEN.EQ.0))THEN + CALL SHOWFU(KBOUT,'Examine quality') +C SCAN + IDIM1=MAXSEQ + CALL DBSCAN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1, + + IDIM1,GEL,IDBSIZ,TEMP3,2,ECHRSZ,MAXGL2, + + KBIN,KBOUT,DEVNOS(2),IDEV,LINLEN,PERCD, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MAXGEL,GEL3,GEL4, + + MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX,DEVNOS(3), + + LLINO,LINCON,LREG,RREG) + GO TO 10 + END IF + IF((NOPT.EQ.8).AND.(IOPEN.EQ.0))THEN + CALL SHOWFU(KBOUT,'Make consensus') +C CONSENSUS + IDIM1=MAXSEQ + CALL CONSEN(RELPG,LNGTHG,LNBR,RNBR,NAMPRO,NGELS,NCONTS,SEQ1, + + IDIM1,GEL,IDBSIZ,TEMP1,ECHRSZ,MAXGL2, + + KBIN,KBOUT,DEVNOS(2),DEVNOS(4),FILNAM, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),MAXGEL,IDM,PERCD, + + DEVNOS(3),LLINO) + CLOSE(UNIT=DEVNOS(4)) + GO TO 10 + END IF + IF(NOPT.EQ.7)THEN + CALL SHOWFU(KBOUT,'Redirect output') + CALL REDIR(IDEV,DEVNOS(6),DEVNOS(10),IGORT,FILNAM,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),KOPT) + GO TO 10 + END IF +C HELP + IF(NOPT.EQ.1)THEN + CALL SHOWFU(KBOUT,'Help') +C HELP + CALL HELP(HELPS,HELPE,BOTOPT,TOPOPT, + + HELPF,DEVNOS(5),KBIN,KBOUT) + GO TO 10 + END IF + IF(NOPT.EQ.27)THEN + CALL SHOWFU(KBOUT,'Set parameters') +C SET DISPLAY PARAMETERS + MN = 10 + MX = 100 + LINLEN = 50 + CALL GETINT(MN,MX,LINLEN,'Display line length', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) LINLEN = IVAL + IPCMIN = 51 + IPCMAX = 100 + IPCD = 75 + CALL GETINT(IPCMIN,IPCMAX,IPCD, + + 'Percentage score for consensus', + + IPVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) THEN + PERCD = REAL(IPVAL)/100. + END IF + MN = 1 + MX = MAXSEQ + CALL GETINT(MN,MX,WINDOW,'Text window length', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) WINDOW = IVAL + MN = 1 + MX = MAXSEQ + CALL GETINT(MN,MX,IGWIND,'Graphics window length', + + IVAL,KBIN,KBOUT,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IOK) + IF(IOK.EQ.0) IGWIND = IVAL + GO TO 10 + END IF + IF(NOPT.EQ.6)THEN + CALL SHOWFU(KBOUT,'Display text file') + CALL TTEXT(DEVNOS(4),FILNAM,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + CLOSE(UNIT=DEVNOS(4)) + GO TO 10 + END IF + IF(NOPT.EQ.28) THEN + CALL SHOWFU(KBOUT,'Highlight disagreements') + CALL HIGHLT(SEQ1,SEQ1(6001),SEQ1(10001),KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),DEVNOS(7), + + DEVNOS(8),FILE,IOK) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + GO TO 10 + END IF + IF((NOPT.EQ.34).AND.(IOPEN.EQ.0))THEN + CALL SHOWFU(KBOUT,'Plot contigs') +C PLOT ALL CONTIGS + CALL PLC(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,0,0, + + NCONTS,MARGL,MARGR,MARGB(NOPT),MARGT(NOPT),ISXMAX,ISYMAX) + GO TO 10 + END IF +C AUTO EDIT + IF((NOPT.EQ.30).AND.(IOPEN.EQ.0))THEN + CALL SHOWFU(KBOUT,'AutoEdit a contig') + WRITE(KBOUT,*)'Make a copy first!' + CALL GETLN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + + LLINO,IERR,IDBSIZ,KBIN,KBOUT,DEVNOS(3), + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + IF(IERR.EQ.0)THEN + CALL GETREG(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + + 1,RELPG(LINCON),LREG,RREG,LINCON,LLINO,IDBSIZ,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),IERR) + IF(IERR.EQ.0) THEN + IDIM1=MAXSEQ + CALL SUMMER(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,SEQ1,IDIM1, + + GEL,LREG,RREG,LLINO,IDBSIZ,TEMP1,ECHRSZ,MAXGL2, + + DEVNOS(2),MAXGEL,IDM,PERCD) + CALL AEDIT(RELPG,LNGTHG,LNBR,RNBR,IDBSIZ,LLINO,LINCON, + + GEL,MAXGEL,SEQ1,IDIM1,DEVNOS(2),DEVNOS(1),LREG,RREG, + + KBOUT) + END IF + END IF + GO TO 10 + END IF + IF((NOPT.EQ.9).AND.(IOPEN.EQ.0)) THEN + CALL SHOWFU(KBOUT,'Screen Edit a contig') + CALL CEDT(KBIN,KBOUT, + + POSNS(1),POSNS(1001),POSNS(2001), + + MAXDB,POSNS(3001),POSNS(3101),50, + + RELPG,LNGTHG,LNBR,RNBR, + + IDBSIZ,NGELS,NCONTS,GEL,GEL2,MAXGEL,LINCON,PERCD,IDM, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),DEVNOS(4), + + DEVNOS(1),DEVNOS(2),DEVNOS(3),LINLEN,FILNAM,LLINO,IOK) + GO TO 10 + END IF + IF(NOPT.EQ.17) THEN + CALL SHOWFU(KBOUT,'Screen for restriction sites') + CALL SCRENR(GEL2,MAXGEL,GEL3,NAMARC,FILE, + + DEVNOS(4),DEVNOS(7),DEVNOS(8),DEVNOS(9),IDEV,KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + CLOSE(UNIT=DEVNOS(4)) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + CLOSE(UNIT=DEVNOS(9)) + GO TO 10 + END IF + IF(NOPT.EQ.18) THEN + CALL SHOWFU(KBOUT,'Screen against vector') + CALL SCRENV(MAXGEL,WORDP,WORDN,LPOWRC,POSNS,GELN, + + SEQ1,MAXSEQ,GEL2,GEL3,GEL4,LENGTH, + + SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON,CONST, + + KBIN,KBOUT,DEVNOS(4),DEVNOS(7),DEVNOS(8),DEVNOS(9),IDEV, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),NAMARC,FILE,IOK) + CLOSE(UNIT=DEVNOS(4)) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + CLOSE(UNIT=DEVNOS(9)) + GO TO 10 + END IF + IF((NOPT.EQ.20).AND.(IOPEN.EQ.0).AND.(IDM.EQ.5)) THEN + CALL SHOWFU(KBOUT,'Auto assemble sequences') + CALL DBAUTO(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ, + +NGELS,NCONTS,MAXGEL, + +TEMP1,WORDP,WORDN,LPOWRC,POSNS,GELN, + +SEQ1,MAXSEQ,GEL2,GEL3,GEL4,SEQ5,SEQC2,SEQG2,GEL, + +MAXGLM,MAXGL2,CHRSIZ,ECHRSZ,LENGTH, + +SAV1,SAV2,SAV3,MAXSAV,CENDS,NENDS,MAXCON,CONST, + +KBIN,KBOUT,DEVNOS(1),DEVNOS(2),DEVNOS(3), + +DEVNOS(4),DEVNOS(7),DEVNOS(8),IDEV, + +HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),NAMARC,NAMPRO,FILE, + +PERCD,IOPEN,IDM,SEQG3,SEQC3,IOK) + CLOSE(UNIT=DEVNOS(4)) + CLOSE(UNIT=DEVNOS(7)) + CLOSE(UNIT=DEVNOS(8)) + GO TO 10 + END IF + IF((NOPT.EQ.32).AND.(IOPEN.EQ.0)) THEN + CALL SHOWFU(KBOUT,'Extract gel readings') + CALL GELOUT(RELPG,LNGTHG,LNBR,RNBR,MAXDB,IDBSIZ,NGELS, + + NCONTS,GEL,MAXGEL,DEVNOS(2),DEVNOS(3),DEVNOS(4),DEVNOS(7), + + KBIN,KBOUT, + + HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5),FILE) + CLOSE(UNIT=DEVNOS(4)) + GO TO 10 + END IF + IF(NOPT.EQ.31) THEN + CALL SHOWFU(KBOUT,'Type in gel readings') + CALL BATIN(SEQ1,MAXSEQ,KBIN,KBOUT, + + DEVNOS(4),DEVNOS(7),IDEV, + + FILNAM,HELPS(NOPT),HELPE(NOPT),HELPF,DEVNOS(5)) + GO TO 10 + END IF + IF((NOPT.GT.3).AND.(IOPEN.NE.0))THEN + WRITE(KBOUT,1012) +1012 FORMAT(' You have not opened a database!') + GO TO 10 + END IF + GO TO 10 +9999 CONTINUE + CALL SHUTD + END + SUBROUTINE DBCOPF(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,GEL, + +NAMPRO,IDEV,IDBSIZ,IERR,KBIN,KBOUT,IDEVR,IDEVW,IDEVN, + +IHELPS,IHELPE,FILEH,IDEVH,MAXGEL,MAXDB,IDM) + CHARACTER FILEH*(*) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ) + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER NAMPRO*(*),NAMARC*10,GEL(MAXGEL) +C CHARACTER NAMFIL*12,MTYPE*4 + INTEGER IWORD + PARAMETER (IWORD=4) + IERR=1 +1 CONTINUE + L = 1 + CALL BUSY(KBOUT) + NEWSIZ = IVAL + LLL = INDEX(NAMPRO,'.') + 1 + NAMPRO(LLL:)='FLAT' + CALL OPENRS(IDEV,NAMPRO,IOK,LRECL,1) + IF(IOK.NE.0)GO TO 100 + IRAW = 0 + IF(IDEVRD.LT.0) IRAW = 1 + WRITE(IDEV,1000,ERR=999)IDBSIZ,MAXGEL,IDM,NGELS,NCONTS,IRAW + 1000 FORMAT(6I6) + DO 10 I=1,NGELS + WRITE(IDEV,1000,ERR=999)RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) +10 CONTINUE + N=IDBSIZ-NCONTS + DO 15 I=N,IDBSIZ-1 + WRITE(IDEV,1000,ERR=999)RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) +15 CONTINUE +C + DO 20 I=1,NGELS + CALL READN(IDEVN,I,NAMARC) + CALL READW(IDEVW,I,GEL,MAXGEL) + WRITE(IDEV,1001,ERR=999)NAMARC + WRITE(IDEV,1002,ERR=999)(GEL(K),K=1,ABS(LNGTHG(I))) + WRITE(IDEV,1004) +20 CONTINUE + 1001 FORMAT(A) + 1002 FORMAT(64A1) + 1004 FORMAT('/') +C IF(IDEVRD.GT.0) THEN +C DO 30 I=1,NGELS +C CALL READRD(IDEVRD,I,LENR,LCUT,LENW,MTYPE,NAMFIL) +C WRITE(IDEV,1003,ERR=999)LENR,LCUT,LENW,MTYPE,NAMFIL +C 30 CONTINUE +C 1003 FORMAT(3I6,' ',A,' ',A) +C END IF + IERR=0 + RETURN +100 CONTINUE + WRITE(KBOUT,9998) + 9998 FORMAT(' Error opening file, copy aborted') + RETURN + 999 CONTINUE + WRITE(KBOUT,9999) +9999 FORMAT(' Error writing data, copy aborted') + RETURN + END + SUBROUTINE DBSTAF(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,NAMPRO,GEL, + +IDBSIS,IDBSIZ,IERR,KBIN,KBOUT,IDEVR,IDEVW,IDEVN,MAXGEL, + +MAXGLM,LLINO, + +IDM,IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER GEL(MAXGLM) + INTEGER RELPG(IDBSIS) + INTEGER LNGTHG(IDBSIS),LNBR(IDBSIS),RNBR(IDBSIS) + CHARACTER NAMPRO*(*),MTYPE*4,NAMFIL*12 + INTEGER IWORD + PARAMETER (IWORD=4) +C SETTING DEVICE NO HERE !!!!!!!!!!!!! + IDEV = 33 + NAMPRO(1:)=' ' + IERR=1 +1 CONTINUE + L = 0 + CALL GTSTR('Project name of flat database', + +' ',NAMPRO,L,KBOUT,KBIN,INFLAG) + IF(L.LT.1) RETURN + LL = L + CALL CCASE(NAMPRO,1) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + LL = INDEX(NAMPRO,' ') + NAMPRO(LL:)='.FLAT' + CALL OPENRS(IDEV,NAMPRO,IOK,LRECL,2) + IF(IOK.NE.0) RETURN + LL = LL - 1 + NAMPRO(LL+1:LL+5)='.RL0 ' + CALL OPENRS(IDEVR,NAMPRO,IOK,4,3) + IF(IOK.NE.0)GO TO 100 + READ(IDEV,1000,ERR=999)IDBSIZ,MAXGEL,IDM,NGELS,NCONTS,IRAW + 1000 FORMAT(6I6) + DO 10 I=1,NGELS + READ(IDEV,1000,ERR=999)RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + 10 CONTINUE + MXT = 0 + DO 20 I=IDBSIZ-NCONTS,IDBSIZ-1 + READ(IDEV,1000,ERR=999)RELPG(I),LNGTHG(I),LNBR(I),RNBR(I) + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + IF(RELPG(I).GT.MXT) THEN + MXT = RELPG(I) + LLINO = LNBR(I) + END IF + 20 CONTINUE + IR = 0 + IL = 0 + DO 30 I=NGELS+1,IDBSIZ-NCONTS-1 + RELPG(I) = 0 + LNGTHG(I) = 0 + LNBR(I) = 0 + RNBR(I) = 0 + CALL WRITER(IDEVR,I,RELPG(I),LNGTHG(I),LNBR(I),RNBR(I)) + 30 CONTINUE + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,IR,IL) +5 CONTINUE + MN = 512 + MX = MAXGLM + CALL GETINT(MN,MX,MAXGEL, + +'Maximum gel reading length', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MAXGEL = IVAL + IF(MOD(IVAL,MN).NE.0) THEN + MAXGEL = 512 + (IVAL/512)*512 + WRITE(KBOUT,*)'Maximum set to',MAXGEL + END IF + IREC=MAXGEL/IWORD + IF(MOD(MAXGEL,IWORD).NE.0)IREC=IREC+1 + NAMPRO(LL+2:LL+3) = 'SQ' + CALL OPENRS(IDEVW,NAMPRO,IOK,IREC,3) + IF(IOK.NE.0)GO TO 100 + CALL FILLC(GEL,MAXGEL,' ') + CALL WRITEW(IDEVW,IDBSIZ,GEL,MAXGEL) + NAMPRO(LL+2:LL+3)='AR' + CALL OPENRS(IDEVN,NAMPRO,IOK,3,3) + IF(IOK.NE.0)GO TO 100 +C NOTE HERE IS A WRITE TO NAMES FILE NOT USING A SUBROUTINE CALL + WRITE(IDEVN,REC=IDBSIS)IDBSIZ,MAXGEL,IDM + DO 40 I = 1,NGELS + READ(IDEV,1001,ERR=999)NAMFIL + CALL WRITEN(IDEVN,I,NAMFIL) + K1 = -63 + CALL FILLC(GEL,MAXGEL,' ') + 35 CONTINUE + K1 = K1 + 64 + K1 = MIN(K1,MAXGEL) + K2 = K1 + 63 + K2 = MIN(K2,MAXGEL) + READ(IDEV,1002,ERR=999)(GEL(K),K=K1,K2) + IF(GEL(K1).NE.'/') GO TO 35 + CALL WRITEW(IDEVW,I,GEL,MAXGEL) + 40 CONTINUE + 1001 FORMAT(A) + 1002 FORMAT(64A1) + IRAW = 1 + IF(IRAW.EQ.0) THEN + NAMPRO(LL+2:LL+3)='RD' + CALL OPENRS(IDEVRD,NAMPRO,IOK,7,3) + ELSE + IDEVRD = -1 + END IF + IF(IDEVRD.GT.0) THEN + DO 50 I=1,NGELS + READ(IDEV,1003,ERR=999)II,J,K,MTYPE,NAMFIL +C CALL WRITRD(IDEVRD,I,II,J,K,MTYPE,NAMFIL) + 50 CONTINUE + 1003 FORMAT(3I6,1X,A,1X,A) + END IF + WRITE(KBOUT,1004)NAMPRO(1:LL),IDBSIZ + 1004 FORMAT(' Database ',A,' version 0, size',I5, + +' successfully started') + IERR=0 + NAMPRO(LL+2:LL+2) = '0' + CLOSE(UNIT=IDEV) + RETURN + 999 CONTINUE + WRITE(KBOUT,*)'Error reading flat file' + CLOSE(UNIT=IDEV) + RETURN +100 CONTINUE + WRITE(KBOUT,9999) +9999 FORMAT(' Error when trying to open database,', + +' database not started') + CLOSE(UNIT=IDEV) + RETURN + END diff --git a/src/staden/sapmen.f b/src/staden/sapmen.f new file mode 100644 index 0000000..a05c586 --- /dev/null +++ b/src/staden/sapmen.f @@ -0,0 +1,188 @@ + SUBROUTINE DBMENT(MENU,NOPT,MAXOPT, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + CHARACTER HELPF*(*) + INTEGER IHELPS(0:MAXOPT),IHELPE(0:MAXOPT) +1 CONTINUE + IF(MENU.EQ.0) THEN + WRITE(KBOUT,5000) +5000 FORMAT(' Menus and their numbers are',/, + + ' m0 = This menu',/, + + ' m1 = General',/, + + ' m2 = Screen control',/, + + ' m3 = Modification',/, + + ' ? = Help',/, + + ' ! = Quit') + ELSE IF(MENU.EQ.-1) THEN + WRITE(KBOUT,1001) +1001 FORMAT(' General menu',/, + +' 0 = List of menus',/, + +' ? = Help',/, + +' ! = Quit',/, + +' 3 = Open a database',/, + +' 5 = Display a contig',/, + +' 6 = List a text file',/, + +' 7 = Redirect output',/, + +' 8 = Calculate a consensus',/, + +' 17 = Screen against restriction enzymes',/, + +' 18 = Screen against vector',/, + +' 19 = Check database',/, + +' 24 = Copy database',/, + +' 25 = Show relationships',/, + +' 27 = Set parameters',/, + +' 28 = Highlight disagreements',/, + +' 29 = Examine quality',/, + +' 35 = Find internal joins') + ELSE IF(MENU.EQ.-2) THEN + WRITE(KBOUT,1002) +1002 FORMAT(' Screen control menu',/, + +' 0 = List of menus',/, + +' ? = Help',/, + +' ! = Quit',/, + +' 10 = Clear graphics',/, + +' 11 = Clear text',/, + +' 12 = Draw ruler',/, + +' 13 = Use cross hair',/, + +' 14 = Change margins',/, + +' 15 = Label diagram',/, + +' 16 = Plot map',/, + +' 33 = Plot single contig',/, + +' 34 = Plot all contigs') + ELSE IF(MENU.EQ.-3) THEN + WRITE(KBOUT,1003) +1003 FORMAT(' Modification menu',/, + +' 0 = List of menus',/, + +' ? = Help',/, + +' ! = Quit',/, + +' 4 = Edit contig',/, + +' 9 = Screen edit',/, + +' 20 = Auto assemble',/, + +' 21 = Enter new gel reading',/, + +' 22 = Join contigs',/, + +' 23 = Complement a contig',/, + +' 26 = Alter relationships',/, + +' 30 = Auto edit a contig',/, + +' 31 = Type in gel readings',/, + +' 32 = Extract gel readings') + END IF + WRITE(KBOUT,1010) + CALL GETOPT(KBIN,KOPT,NOPT) + IF((NOPT.LT.-3).OR.(NOPT.GT.MAXOPT).OR.(KOPT.GT.2)) THEN + MENU = 0 + GO TO 1 + END IF + IF((KOPT.EQ.2).AND.(NOPT.GE.0))THEN + CALL HELP2(IHELPS(NOPT),IHELPE(NOPT),HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(NOPT.EQ.0) THEN + MENU = 0 + GO TO 1 + END IF + IF(NOPT.LT.0) THEN + MENU = NOPT + GO TO 1 + END IF +1010 FORMAT( + +' ? Option number=',$) + RETURN + END + SUBROUTINE DBMENU(MENU,NOPT,IHELPS,IHELPE,HELPF,IDEVH, + +KBIN,KBOUT) + CHARACTER HELPF*(*) + INTEGER IHELPS,IHELPE +1 CONTINUE + IF(MENU.EQ.2)THEN +20 CONTINUE + CALL BPAUSE(KBIN,KBOUT,IOK) + WRITE(KBOUT,1002) +1002 FORMAT( ' Enter menu options are:',/, + +' ? = Help',/, + +' ! = Give up',/, + +' 3 = Complete entry',/, + +' 4 = Edit contig',/, + +' 5 = Display',/, + +' 6 = Edit new gel reading') + WRITE(KBOUT,1010) + CALL GETOPT(KBIN,KOPT,NOPT) + MAXOPT = 6 + IF((NOPT.LT.0).OR.(NOPT.GT.MAXOPT).OR.(KOPT.GT.2)) THEN + GO TO 20 + END IF + IF((KOPT.EQ.2).AND.(NOPT.GE.0))THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 20 + END IF + ELSE IF(MENU.EQ.3)THEN +30 CONTINUE + CALL BPAUSE(KBIN,KBOUT,IOK) + WRITE(KBOUT,1003) +1003 FORMAT( ' Join menu options are:'/ + +' ? = Help',/, + +' ! = Give up',/, + +' 3 = Complete join',/, + +' 4 = Edit left contig',/, + +' 5 = Display join',/, + +' 6 = Edit right contig',/, + +' 7 = Move join') + WRITE(KBOUT,1010) + CALL GETOPT(KBIN,KOPT,NOPT) + MAXOPT = 7 + IF((NOPT.LT.0).OR.(NOPT.GT.MAXOPT).OR.(KOPT.GT.2)) THEN + GO TO 30 + END IF + IF((KOPT.EQ.2).AND.(NOPT.GE.0))THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 30 + END IF + ELSE IF(MENU.EQ.4)THEN +40 CONTINUE + CALL BPAUSE(KBIN,KBOUT,IOK) + WRITE(KBOUT,1004) +1004 FORMAT(' Alter relationships menu options are:'/ + +' ? = Help',/, + +' ! = Quit',/, + +' 3 = Line change',/, + +' 4 = Edit gel reading',/, + +' 5 = Delete contig line',/, + +' 6 = Shift',/, + +' 7 = Move gel reading',/, + +' 8 = Rename gel reading',/, + +' 9 = Break a contig',/, + +' 10 = Remove a gel reading') + WRITE(KBOUT,1010) + CALL GETOPT(KBIN,KOPT,NOPT) + MAXOPT = 10 + IF((NOPT.LT.0).OR.(NOPT.GT.MAXOPT).OR.(KOPT.GT.2)) THEN + GO TO 40 + END IF + IF((KOPT.EQ.2).AND.(NOPT.GE.0))THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 40 + END IF + ELSE IF(MENU.EQ.5)THEN +50 CONTINUE + CALL BPAUSE(KBIN,KBOUT,IOK) + WRITE(KBOUT,1005) +1005 FORMAT( + +' Edit options are:',/, + +' ? = Help',/, + +' ! = Quit',/, + +' 3 = Insert',/, + +' 4 = Delete',/, + +' 5 = Change') + WRITE(KBOUT,1010) + CALL GETOPT(KBIN,KOPT,NOPT) + MAXOPT = 5 + IF((NOPT.LT.0).OR.(NOPT.GT.MAXOPT).OR.(KOPT.GT.2)) THEN + GO TO 50 + END IF + IF((KOPT.EQ.2).AND.(NOPT.GE.0))THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 50 + END IF + END IF +1010 FORMAT( + +' ? Option number=',$) + IF(NOPT.LE.0) GO TO 1 + RETURN + END diff --git a/src/staden/searchUtils.c b/src/staden/searchUtils.c new file mode 100644 index 0000000..216518d --- /dev/null +++ b/src/staden/searchUtils.c @@ -0,0 +1,1254 @@ +/* +** Search functions for the contig editor +** +** This file is split into two parts, probably implying that it should be +** divided into two files. The first half consists of all the search +** engines, the second half the user interface code. +** +** Observations: +** Only one search window can be up at one time. +** +** Changes: +** +** 7-Nov-1991 SD Select the tag when searching on tag. +** 20-Feb-1992 SD Renamed "OK" button to "Quit" +** 29-Apr-1992 SD Changes relevant to general speedup in edUtils.c +** 11-May-1992 SD Search by name now dependant on cursor position +** 15-May-1992 SD now NOSTRSTR and NOSTRDUP +** +*/ + + +#define REDISPLAY(X) setDisplayPos((X),positionInContig((X),(X)->cursorSeq,(X)->cursorPos)) + +/* +** The first half +*/ +#include +#include +#include "edUtils.h" +#include "tagUtils.h" +#include "searchUtils.h" +#include "select.h" +#include "string.h" +#include "ctype.h" +#include "misc.h" + +/* +** Prototypes +*/ +extern char *re_comp(char *s); +extern int re_exec(char *s); + +#define in_interval(A,B,C) ( ((A)<(B))?((A)<=(C) && (C)<=(B)):((B)<=(C) && (C)<=(A)) ) + +/* +** We don't want to have all the textwidget translations +** Here are the list of the ones we want. +** This is taken from Xaw/TextTr.c source +*/ +static char *defaultTranslations = "\ +Right: forward-character() \n\ +Left: backward-character() \n\ +Delete: delete-previous-character() \n\ +BackSpace: delete-previous-character() \n\ +: insert-char() \n\ +: focus-in() \n\ +: focus-out() \n\ +: select-start() \n\ +: extend-adjust() \n\ +: extend-end(PRIMARY, CUT_BUFFER0) \n\ +: insert-selection(PRIMARY, CUT_BUFFER0) \n\ +: extend-start() \n\ +: extend-adjust() \n\ +: extend-end(PRIMARY, CUT_BUFFER0) \ +"; + +static void my_translations(Widget w) +/* +** Use my default translations +*/ +{ + XtTranslations parsedTranslations; + + parsedTranslations = XtParseTranslationTable(defaultTranslations); + + XtUninstallTranslations(w); + XtAugmentTranslations(w,parsedTranslations); +} + +static int findGelByNumber (EdStruct *xx, char *s) +/* +** Position cursor on left end of gel sequence matched by string s. +** If s starts with a slash '/' assume a gel name is specified. +** Otherwise assume a gel number is specified. +*/ +{ + int i; + + int gel; + gel = atoi(s); + for (i=1; i <= xx->DB_gelCount; i++) { + if (DBgetNumber(xx,i) == gel) { + xx->cursorSeq = i; + xx->cursorPos = 1; + REDISPLAY(xx); + return 1; + } + } + + return 0; +} + +static int findNextByName(EdStruct *xx, char *s) +/* +** Search forwards from the cursor position until the sequence specified +** is found. The cursor is positioned at the left end of the sequence, +** if found. +** +*/ +{ + int spos = positionInContig(xx,xx->cursorSeq,xx->cursorPos)+1; + int i; + int n; + + if (!*s) return 0; + n = strlen(s); + + if ((i = posToIndex(xx,spos))==0) return 0; + + for (; i <= xx->DB_gelCount; i++) { + /* search through tag list for sequence */ + + if (strncmp(DBgetGelName(xx,xx->DBorder[i]),s,n)==0) { + xx->cursorSeq = xx->DBorder[i]; + xx->cursorPos = 1; + REDISPLAY(xx); + return 1; + } + } + + + return 0; +} + + +static int findPrevByName(EdStruct *xx, char *s) +/* +** Search forwards from the cursor position until the sequence specified +** is found. The cursor is positioned at the left end of the sequence, +** if found. +** +*/ +{ + int spos = positionInContig(xx,xx->cursorSeq,xx->cursorPos)-1; + int i; + int n; + + if (!*s) return 0; + n = strlen(s); + + if ((i = posToIndex(xx,spos))==0) return 0; + + for (i--; i >= 0 ; i--) { + /* search through tag list for sequence */ + + if (strncmp(DBgetGelName(xx,xx->DBorder[i]),s,n)==0) { + xx->cursorSeq = xx->DBorder[i]; + xx->cursorPos = 1; + REDISPLAY(xx); + return 1; + } + } + + + return 0; +} + +static int findNextGelByName (EdStruct *xx, char *s) +/* +** Position cursor on left end of gel sequence matched by string s. +** If s starts with a slash '/' assume a gel name is specified. +** Otherwise assume a gel number is specified. +*/ +{ + if (*s) { + if (*s == '/') { + s++; + return findNextByName(xx,s); + } else + return findGelByNumber(xx,s); + } + return 0; +} + + +static int findPrevGelByName (EdStruct *xx, char *s) +/* +** Position cursor on left end of gel sequence matched by string s. +** If s starts with a slash '/' assume a gel name is specified. +** Otherwise assume a gel number is specified. +*/ +{ + if (*s) { + if (*s == '/') { + s++; + return findPrevByName(xx,s); + } else + return findGelByNumber(xx,s); + } + return 0; +} + + + + + +static int findNextTagByType (EdStruct *xx, char *type) +/* +** Search forwards from the cursor position until a tag of a specified +** type is encountered. The cursor is positioned at the left end of the +** tag, if found. +*/ +{ + int spos = positionInContig(xx,xx->cursorSeq,xx->cursorPos)+1; + int epos = DBgetLength(xx,0); + int fpos,fseq,i; + int fseqpos; + int *seqList; + tagStruct *found_tag; + + seqList = sequencesInRegion(xx,spos, epos); + fseq = 0; + fseqpos = 0; + fpos = epos + 1; + + for (i=0; seqList[i] && DBgetRelPos(xx,seqList[i]) < fpos ; i++) { + /* search through tag list for sequence */ + int seq = seqList[i]; + tagStruct *t; + + t = (tagStruct *) DBgetTags(xx,seq); + while (t != NULL) { + int normpos; + int tagpos; + normpos = normalisePos(xx,seq,t->tagrec.position,t->tagrec.length); + tagpos=positionInContig(xx,seq,normpos); + if (!(t->flags & TAG_DELETED) && + in_interval(spos,fpos,tagpos) && + strncmp(t->tagrec.type.c,type,4)==0) { + fseq = seq; + fseqpos = normpos; + fpos = tagpos; + found_tag = t; + if (DBgetComp(xx,seq) == COMPLEMENTED) + /* Keep looking */ + t = t->next; + else + /* Stop now */ + t = NULL; + } else + t = t->next; + } + + } + + + if (fseq) { + xx->cursorSeq = fseq; + xx->cursorPos = fseqpos; + _select_tag(xx,fseq,found_tag); + REDISPLAY(xx); + } + + return fseq; +} + + + + +static int findPrevTagByType (EdStruct *xx, char *type) +/* +** Search backwards from the current cursor position until a tag of the +** specified type is encountered. The cursor is positioned at the left +** end of the tag, if found. +*/ +{ + int spos = positionInContig(xx,xx->cursorSeq,xx->cursorPos)-1; + int epos = 1; + int fpos,fseq,i; + int fseqpos; + int *seqList; + tagStruct *found_tag; + + seqList = sequencesInRegion(xx,epos, spos); + fseq = 0; + fseqpos = 0; + fpos = epos - 1; + + for (i=0; seqList[i]; i++) ; + for (i--; i>=0 && DBgetRelPos(xx,seqList[i])+DBgetLength(xx,seqList[i]) > fpos ; i--) { + /* search through tag list for sequence */ + int seq = seqList[i]; + tagStruct *t; + + t = (tagStruct *) DBgetTags(xx,seq); + while (t != NULL) { + int normpos; + int tagpos; + normpos = normalisePos(xx,seq,t->tagrec.position,t->tagrec.length); + tagpos = positionInContig(xx,seq,normpos); + if (!(t->flags & TAG_DELETED) && + in_interval(spos,fpos,tagpos) && + strncmp(t->tagrec.type.c,type,4)==0) { + fseq = seq; + fseqpos = normpos; + fpos = tagpos; + found_tag = t; + if (DBgetComp(xx,seq) != COMPLEMENTED) + /* Keep looking */ + t = t->next; + else + /* Stop now */ + t = NULL; + } else + t = t->next; + } + + } + + + if (fseq) { + xx->cursorSeq = fseq; + xx->cursorPos = fseqpos; + _select_tag(xx,fseq,found_tag); + REDISPLAY(xx); + } + + return fseq; +} + + + + + +static int findNextSequence(EdStruct *xx, char *s) +/* +** Search forwards from the cursor position until the sequence specified +** is found. The cursor is positioned at the left end of the sequence, +** if found. +** +** Observations: +** The search is done on a reading by reading basis, rather than from +** the consensus. +** The search is not case sensitive. +*/ +{ + int spos = positionInContig(xx,xx->cursorSeq,xx->cursorPos)+1; + int epos = DBgetLength(xx,0); + int fpos,fseq,i; + int fseqpos; + int *seqList; + char *reading; + int maxlen; + + seqList = sequencesInRegion(xx,spos, epos); + fseq = 0; + fseqpos = 0; + fpos = epos + 1; + + + for (maxlen = 0, i=0; seqList[i] ; i++) + maxlen = max(maxlen, DBgetLength(xx,seqList[i])); + + reading = malloc(maxlen+1); + + for (i=0; seqList[i] && DBgetRelPos(xx,seqList[i]) < fpos ; i++) { + /* search through tag list for sequence */ + int seq = seqList[i]; + char *str; + char *ind; + + str = DBgetSeq(xx,seq); + strncpy(reading,str,DBgetLength(xx,seq)); + reading[DBgetLength(xx,seq)] = '\0'; + + if (in_interval(1,DBgetLength(xx,seq),spos - DBgetRelPos(xx,seq)+1)) { + int offset = spos - DBgetRelPos(xx,seq); + ind = strstr(reading + offset,s); + } else + ind = strstr(reading,s); + + if (ind != NULL) { + int pos; + pos = positionInContig(xx,seq,(int) (ind - reading) + 1); + if (in_interval(spos,fpos,pos)) { + fseqpos = (int) (ind - reading) + 1; + fpos = pos; + fseq = seq; + } + + } + + } + + if (fseq) { + xx->cursorSeq = fseq; + xx->cursorPos = fseqpos; + REDISPLAY(xx); + } + + free(reading); + return fseq; +} + + + + +static void reverse_str(char *s, int len) +/* +** Verse the character sequence of a string +*/ +{ + int i; + char temp; + + for (i=0; i < len/2; i++) { + temp = s[i]; + s[i] = s[len-i-1]; + s[len-i-1] = temp; + } + +} + + + + +static int findPrevSequence(EdStruct *xx, char *inseq) +/* +** Search backwards from the cursor position until the sequence specified +** is found. The cursor is positioned at the left end of the sequence, +** if found. +** +** Observations: +** The search is done on a reading by reading basis, rather than from +** the consensus. +** The search is not case sensitive. +*/ +{ + int spos = positionInContig(xx,xx->cursorSeq,xx->cursorPos)+strlen(inseq)-2; + int epos = 1; + int fpos,fseq,i; + int fseqpos; + int *seqList; + char *reading; + char *s; + int maxlen; + + s = strdup(inseq); + reverse_str(s,strlen(s)); + + seqList = sequencesInRegion(xx,epos, spos); + fseq = 0; + fseqpos = 0; + fpos = epos - 1; + + + for (maxlen = 0, i=0; seqList[i] ; i++) + maxlen = max(maxlen, DBgetLength(xx,seqList[i])); + + reading = malloc(maxlen+1); + + for (i=0; seqList[i]; i++) ; + for (i--; i>=0 && DBgetRelPos(xx,seqList[i])+DBgetLength(xx,seqList[i]) > fpos ; i--) { + /* search through tag list for sequence */ + int seq = seqList[i]; + char *str; + char *ind; + + str = DBgetSeq(xx,seq); + strncpy(reading,str,DBgetLength(xx,seq)); + reading[DBgetLength(xx,seq)] = '\0'; + reverse_str(reading,DBgetLength(xx,seq)); + + if (in_interval(1,DBgetLength(xx,seq),spos - DBgetRelPos(xx,seq)+1)) { + int offset = DBgetLength(xx,seq) - (spos - DBgetRelPos(xx,seq)+1); + ind = strstr(reading + offset,s); + } else + ind = strstr(reading,s); + + if (ind != NULL) { + int pos; + pos = positionInContig(xx, + seq, + DBgetLength(xx,seq) - (int)(ind - reading) - strlen(s) + 1); + if (in_interval(spos,fpos,pos)) { + fseqpos = pos - DBgetRelPos(xx,seq) + 1; + fpos = pos; + fseq = seq; + } + + } + + } + + if (fseq) { + xx->cursorSeq = fseq; + xx->cursorPos = fseqpos; + REDISPLAY(xx); + } + + free(reading); + return fseq; +} + + + + + +static int findNextAnno(EdStruct *xx, char *anno) +/* +** Search forwards from the cursor position until a tag containing the +** specified annotation is found. The cursor is positioned at the left +** end of the tag, if found. +** +** Observations: +** A regular expression search is found, giving unpredictable results +** to people unfamiliar with such searches +*/ +{ + int spos = positionInContig(xx,xx->cursorSeq,xx->cursorPos)+1; + int epos = DBgetLength(xx,0); + int fpos,fseq,i; + int fseqpos; + int *seqList; + char *error; + char *find_all = "$"; + tagStruct *found_tag; + + if (! *anno) anno = find_all; + + error = re_comp(anno); + + if (error != NULL) { + fprintf(stderr,"findNextAnno: %s\n",error); + return 0; + } + + + seqList = sequencesInRegion(xx,spos, epos); + fseq = 0; + fseqpos = 0; + fpos = epos + 1; + + for (i=0; seqList[i] && DBgetRelPos(xx,seqList[i]) < fpos ; i++) { + /* search through tag list for sequence */ + int seq = seqList[i]; + tagStruct *t; + + t = (tagStruct *) DBgetTags(xx,seq); + while (t != NULL) { + int normpos; + int tagpos; + int match; + /* Code isn't very efficient here! */ + normpos = normalisePos(xx,seq,t->tagrec.position,t->tagrec.length); + tagpos=positionInContig(xx,seq,normpos); + /* + ** Get annotation + */ + force_comment(t); + match = ( re_exec(t->newcomment) == 1); + if (!(t->flags & TAG_DELETED) && + in_interval(spos,fpos,tagpos) && + t->tagrec.type.c[0] != '*' && /* avoid special tags */ + t->tagrec.position != 0 && + match) { + fseq = seq; + fseqpos = normpos; + fpos = tagpos; + found_tag = t; + if (DBgetComp(xx,seq) == COMPLEMENTED) + /* Keep looking */ + t = t->next; + else + /* Stop now */ + t = NULL; + } else + t = t->next; + } + + } + + + if (fseq) { + xx->cursorSeq = fseq; + xx->cursorPos = fseqpos; + _select_tag(xx,fseq,found_tag); + REDISPLAY(xx); + } + + return fseq; +} + +static int findPrevAnno(EdStruct *xx, char *anno) +/* +** Search backwards from the cursor position until a tag containing the +** specified annotation is found. The cursor is positioned at the left +** end of the tag, if found. +** +** Observations: +** A regular expression search is found, giving unpredictable results +** to people unfamiliar with such searches +*/ +{ + int spos = positionInContig(xx,xx->cursorSeq,xx->cursorPos)-1; + int epos = 1; + int fpos,fseq,i; + int fseqpos; + int *seqList; + char *error; + char *find_all = "$"; + tagStruct *found_tag; + + if (! *anno) anno = find_all; + + error = re_comp(anno); + + if (error != NULL) { + fprintf(stderr,"findPrevAnno: %s\n",error); + return 0; + } + + + seqList = sequencesInRegion(xx,epos, spos); + fseq = 0; + fseqpos = 0; + fpos = epos - 1; + + for (i=0; seqList[i]; i++) ; + for (i--; i>=0 && DBgetRelPos(xx,seqList[i])+DBgetLength(xx,seqList[i]) > fpos ; i--) { + /* search through tag list for sequence */ + int seq = seqList[i]; + tagStruct *t; + + t = (tagStruct *) DBgetTags(xx,seq); + while (t != NULL) { + int normpos; + int tagpos; + int match; + /* Code isn't very efficient here! */ + normpos = normalisePos(xx,seq,t->tagrec.position,t->tagrec.length); + tagpos=positionInContig(xx,seq,normpos); + /* + ** Get annotation + */ + force_comment(t); + match = ( re_exec(t->newcomment) == 1); + if (!(t->flags & TAG_DELETED) && + in_interval(spos,fpos,tagpos) && + t->tagrec.type.c[0] != '*' && /* avoid special tags */ + t->tagrec.position != 0 && + match) { + fseq = seq; + fseqpos = normpos; + fpos = tagpos; + found_tag = t; + if (DBgetComp(xx,seq) != COMPLEMENTED) + /* Keep looking */ + t = t->next; + else + /* Stop now */ + t = NULL; + } else + t = t->next; + } + + } + + + if (fseq) { + xx->cursorSeq = fseq; + xx->cursorPos = fseqpos; + _select_tag(xx,fseq,found_tag); + REDISPLAY(xx); + } + + return fseq; +} + + + +static int findPosition(EdStruct *xx, char *text_pos) +/* +** Position the cursor at the position specified. +** There are three modes: +** 1. By position in contig. eg 30717 +** 2. By position in a gel. eg @100 +** 3. By a relative offset. eg +1000 eg -1000 +** +** Observations: +** The cursor is positioned in the same gel if possible. +** If it possible to specify negative or large numbers when +** specifying position in gel. +*/ +{ + + int pos; + int cseq = xx->cursorSeq; + int cpos = xx->cursorPos; + + for(; *text_pos && isspace(*text_pos) ; text_pos++) ; + + switch (*text_pos) { + case '\0': + return 0; + case '+': + case '-': + pos = positionInContig(xx,cseq,cpos) + atoi(text_pos); + break; + case '@': + pos = DBgetRelPos(xx,cseq) + atoi(++text_pos) - 1; + break; + default: + pos = atoi(text_pos); + break; + } + + if (pos > 0 && pos <= DBgetLength(xx,0)) { + if ( in_interval(DBgetRelPos(xx,cseq),DBgetRelPos(xx,cseq)+DBgetLength(xx,cseq)-1,pos ) ) { + xx->cursorPos = pos - DBgetRelPos(xx,cseq) + 1; + } else { + xx->cursorSeq = 0; + xx->cursorPos = pos; + } + REDISPLAY(xx); + return 1; + } + return 0; + +} + + + +int findNextProblem (EdStruct *xx) +/* +** Search forward from the cursor position until a the consensus is not +** A, C, G or T. The cursor is positioned on the problem base, if found. +*/ +{ +#define SEARCH_CHUNKS MAX_DISPLAY_WIDTH + int spos = positionInContig(xx,xx->cursorSeq,xx->cursorPos)+1; + int epos = DBgetLength(xx,0); + char buffer[SEARCH_CHUNKS+1]; + int i,width; + + for (i=spos; i<= epos; i+=SEARCH_CHUNKS) { + width = min(epos-i+1,SEARCH_CHUNKS); + DBcalcConsensus (xx,i,width, buffer,BOTH_STRANDS); + { + int check = strcspn(buffer,"-*"); + if (check != width) { /* we have problem! */ + xx->cursorSeq = 0; + xx->cursorPos = i+check; + REDISPLAY(xx); + return 1; + } + } + } + return 0; +} + + + +static int findPrevProblem (EdStruct *xx) +/* +** Search forward from the cursor position until a the consensus is not +** A, C, G or T. The cursor is positioned on the problem base, if found. +*/ +{ +#define SEARCH_CHUNKS MAX_DISPLAY_WIDTH + int spos = positionInContig(xx,xx->cursorSeq,xx->cursorPos)-1; + int epos = 1; + char buffer[SEARCH_CHUNKS+1]; + int i,width; + int j; + + for (i=spos; i>= epos; i-=SEARCH_CHUNKS) { + width = min(i-epos+1,SEARCH_CHUNKS); + DBcalcConsensus (xx,i-width+1,width, buffer, BOTH_STRANDS); + for (j=width-1;j>=0;j--) { + char *check = strchr("-*",buffer[j]); + if (check != NULL) { /* we have problem! */ + xx->cursorSeq = 0; + xx->cursorPos = i-width+1+j; + REDISPLAY(xx); + return 1; + } + } + } + return 0; +} + + +static int qual_prob(char a, char b) +/* +** Returns true if character 'a' is out of sorts with character 'b' +*/ +{ + switch (a) { + case ' ': + return 0; + case '*': + case '-': + return 1; + default: + switch (b) { + case ' ': + return 0; + case '*': + case '-': + return 1; + default: + return (a != b); + } + } +} + + + + +static int findNextQualProb (EdStruct *xx) +/* +** Search forwards from the cursor position until a problem relating to +** quality is found. The cursor is positioned on the problematic base, if found. +** +** Observations: +** Large stretches of sequence on one strand only could cause frustration +*/ +{ +#define SEARCH_CHUNKS MAX_DISPLAY_WIDTH + int spos = positionInContig(xx,xx->cursorSeq,xx->cursorPos)+1; + int epos = DBgetLength(xx,0); + char buffer1[SEARCH_CHUNKS+1]; + char buffer2[SEARCH_CHUNKS+1]; + int i,width; + int j; + + for (i=spos; i<= epos; i+=SEARCH_CHUNKS) { + width = min(epos-i+1,SEARCH_CHUNKS); + DBcalcConsensus (xx,i,width,buffer1,COMPLEMENTED); + DBcalcConsensus (xx,i,width,buffer2,UNCOMPLEMENTED); + for (j=0;jcursorSeq = 0; + xx->cursorPos = i+j; + REDISPLAY(xx); + return 1; + } + } + } + return 0; +} + + + +static int findPrevQualProb (EdStruct *xx) +/* +** Search backwards from the cursor position until a problem relating to +** quality is found. The cursor is positioned on the problematic base, if found. +** +** Observations: +** Large stretches of sequence on one strand only could cause frustration +*/ +{ +#define SEARCH_CHUNKS MAX_DISPLAY_WIDTH + int spos = positionInContig(xx,xx->cursorSeq,xx->cursorPos)-1; + int epos = 1; + char buffer1[SEARCH_CHUNKS+1]; + char buffer2[SEARCH_CHUNKS+1]; + int i,width; + int j; + + for (i=spos; i>= epos; i-=SEARCH_CHUNKS) { + width = min(i-epos+1,SEARCH_CHUNKS); + DBcalcConsensus (xx,i-width+1,width,buffer1,COMPLEMENTED); + DBcalcConsensus (xx,i-width+1,width,buffer2,UNCOMPLEMENTED); + for (j=width-1;j>=0;j--) { + if (qual_prob(buffer1[j],buffer2[j])) { /* we have problem! */ + xx->cursorSeq = 0; + xx->cursorPos = i-width+1+j; + REDISPLAY(xx); + return 1; + } + } + } + return 0; +} + + + + + +/* +** The second half +*/ + +/* +** Widgets and things +*/ +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include + +#include "edMenu.h" +#include "main.h" + +#define FORWARDS 1 +#define BACKWARDS 2 + +static EdStruct *XX; +static char tag_type[4]; +static int up = 0; +static Widget oldFogieWid; + +static Widget searchWid = NULL; +static Widget form; +static Widget label; +static Widget dir_box, backwards, forwards; +static Widget opt_box, by_pos, by_name, by_tag_type, by_tag_val, by_seq, by_prob, by_qual; +static Widget val_box, text, type; +static Widget act_box, search,quit; + + + + +static void searchCallback(Widget w, XtPointer client_data, XtPointer call_data) +/* +** Interrogate buttons to determine search direct and mode, and call +** the appropriate routine. +*/ +{ + char *text_str; + Arg args[10]; + Cardinal nargs; + int found; + + int option, isforwards; + + nargs = 0; + XtSetArg(args[nargs], XtNstring, &text_str); nargs++; + XtGetValues(text,args,nargs); + + option = (int) XawToggleGetCurrent(by_pos); + isforwards = ( FORWARDS == (int) XawToggleGetCurrent(forwards) ); + + if (isforwards) { + + switch(option){ + case OptByGelName: + found = findNextGelByName(XX,text_str); + break; + case OptByAnnotation: + found = findNextAnno(XX,text_str); + break; + case OptBySequence: + found = findNextSequence(XX,text_str); + break; + case OptByTagType: + found = findNextTagByType (XX, tag_type); + break; + case OptByPosition: + found = findPosition(XX,text_str); + break; + case OptByProblem: + found = findNextProblem(XX); + break; + case OptByQuality: + found = findNextQualProb(XX); + break; + } + + } else { + + switch(option){ + case OptByGelName: + found = findPrevGelByName(XX,text_str); + break; + case OptByAnnotation: + found = findPrevAnno(XX,text_str); + break; + case OptBySequence: + found = findPrevSequence(XX,text_str); + break; + case OptByTagType: + found = findPrevTagByType (XX, tag_type); + break; + case OptByPosition: + found = findPosition(XX,text_str); + break; + case OptByProblem: + found = findPrevProblem(XX); + break; + case OptByQuality: + found = findPrevQualProb(XX); + break; + } + + } + + if (!found) XBell(GetDisplay(),100); + +} + + + + +static void quitCallback(Widget w, XtPointer client_data, XtPointer call_data) +/* +** Leave the search window gracefully +*/ +{ + up = 0; + XtPopdown(searchWid); +} + + +#include "tagdb.h" +static void tagMenuCallback(Widget w, XtPointer client_data, XtPointer call_data) +/* +** The changing of the tag type - don't miss it! +*/ +{ + tag_db_struct *t = (tag_db_struct *) client_data; + setButtonName(type,t->type); + strncpy(tag_type,t->id,4); + +} + + + + +static void create_search_wid(Widget parentWid) +/* +** Create the widgets for the search window. +*/ +{ + /* + ** Label + ** Search: [Backwards] [Forwards] + ** Operation: + ** [Position] [Gel Name] + ** [Annotation Type] [Annotation Content] + ** [Sequence] [Problems] + ** Value: [ ] [Type] + ** [Search] [Quit] + */ + + + + Cardinal nargs; + Arg args[10]; + Position x, y; /* top-left hand corner of new widget */ + Dimension height; /* height of parent widget */ + + /* + ** Determine the position on the screen for this widget + */ +#define fromVertWid (XX->edWid) + nargs = 0; + XtSetArg(args[nargs], XtNheight, &height); nargs++; + XtGetValues(fromVertWid, args, nargs); + XtTranslateCoords(fromVertWid, (Position) 0, (Position) height, &x, &y); + + /* + ** Create popup shell + */ + nargs = 0; + XtSetArg(args[nargs], XtNx, x); nargs++; + XtSetArg(args[nargs], XtNy, y); nargs++; + searchWid = XtCreatePopupShell("search", transientShellWidgetClass, parentWid, args, nargs); + + /* + ** Create main form + */ + nargs = 0; + form = XtCreateManagedWidget("form", formWidgetClass, searchWid, args, nargs); + + /* + ** Create title for form + */ + nargs = 0; + XtSetArg(args[nargs], XtNborderWidth, 0); nargs++; + XtSetArg(args[nargs], XtNlabel, "Search Window"); nargs++; + label = XtCreateManagedWidget("label", labelWidgetClass, form, args, nargs); + + /* + ** Create buttons for search direction + */ + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, label); nargs++; + XtSetArg(args[nargs], XtNborderWidth, 0); nargs++; +#define XtOrientHorizontal "horizontal" + XtSetArg(args[nargs], XtNorientation, XtOrientHorizontal); nargs++; + dir_box = XtCreateManagedWidget("dir_box", boxWidgetClass, form, args, nargs); + nargs = 0; + XtSetArg(args[nargs], XtNborderWidth, 0); nargs++; + XtSetArg(args[nargs], XtNlabel, "Direction:"); nargs++; + (void) XtCreateManagedWidget("label", labelWidgetClass, dir_box, args, nargs); + nargs = 0; + XtSetArg(args[nargs],XtNradioData,BACKWARDS); nargs++; + backwards = XtCreateManagedWidget("backwards", toggleWidgetClass, dir_box, args, nargs); + nargs = 0; + XtSetArg(args[nargs],XtNradioGroup, backwards); nargs++; + XtSetArg(args[nargs],XtNstate, True); nargs++; + XtSetArg(args[nargs],XtNradioData,FORWARDS); nargs++; + forwards = XtCreateManagedWidget("forwards", toggleWidgetClass, dir_box, args, nargs); + + /* + ** Create buttons for operation selection + */ + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, dir_box); nargs++; + XtSetArg(args[nargs], XtNborderWidth, 0); nargs++; +#define XtOrientHorizontal "horizontal" + XtSetArg(args[nargs], XtNorientation, XtOrientHorizontal); nargs++; + opt_box = XtCreateManagedWidget("opt_box", boxWidgetClass, form, args, nargs); + nargs = 0; + XtSetArg(args[nargs], XtNborderWidth, 0); nargs++; + XtSetArg(args[nargs], XtNlabel, "Search by:"); nargs++; + (void) XtCreateManagedWidget("label", labelWidgetClass, opt_box, args, nargs); + nargs = 0; + XtSetArg(args[nargs],XtNstate, True); nargs++; + XtSetArg(args[nargs],XtNlabel,"Position"); nargs++; + XtSetArg(args[nargs],XtNradioData,OptByPosition); nargs++; + by_pos = XtCreateManagedWidget("by_pos", toggleWidgetClass, opt_box, args, nargs); + nargs = 0; + XtSetArg(args[nargs],XtNradioGroup, by_pos); nargs++; + XtSetArg(args[nargs],XtNlabel,"Reading Name"); nargs++; + XtSetArg(args[nargs],XtNradioData,OptByGelName); nargs++; + by_name = XtCreateManagedWidget("by_name", toggleWidgetClass, opt_box, args, nargs); + nargs = 0; + XtSetArg(args[nargs],XtNradioGroup, by_pos); nargs++; + XtSetArg(args[nargs],XtNlabel,"Tag Type"); nargs++; + XtSetArg(args[nargs],XtNradioData,OptByTagType); nargs++; + by_tag_type = XtCreateManagedWidget("by_tag_type", toggleWidgetClass, opt_box, args, nargs); + nargs = 0; + XtSetArg(args[nargs],XtNradioGroup, by_pos); nargs++; + XtSetArg(args[nargs],XtNlabel,"Annotation"); nargs++; + XtSetArg(args[nargs],XtNradioData,OptByAnnotation); nargs++; + by_tag_val = XtCreateManagedWidget("by_tag_val", toggleWidgetClass, opt_box, args, nargs); + nargs = 0; + XtSetArg(args[nargs],XtNradioGroup, by_pos); nargs++; + XtSetArg(args[nargs],XtNlabel,"Sequence"); nargs++; + XtSetArg(args[nargs],XtNradioData,OptBySequence); nargs++; + by_seq = XtCreateManagedWidget("by_seq", toggleWidgetClass, opt_box, args, nargs); + nargs = 0; + XtSetArg(args[nargs],XtNradioGroup, by_pos); nargs++; + XtSetArg(args[nargs],XtNlabel,"Problem"); nargs++; + XtSetArg(args[nargs],XtNradioData,OptByProblem); nargs++; + by_prob = XtCreateManagedWidget("by_prob", toggleWidgetClass, opt_box, args, nargs); + nargs = 0; + XtSetArg(args[nargs],XtNradioGroup, by_pos); nargs++; + XtSetArg(args[nargs],XtNlabel,"Quality"); nargs++; + XtSetArg(args[nargs],XtNradioData,OptByQuality); nargs++; + by_qual = XtCreateManagedWidget("by_qual", toggleWidgetClass, opt_box, args, nargs); + + + /* + ** Create selection criteria box + */ + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, opt_box); nargs++; + XtSetArg(args[nargs], XtNborderWidth, 0); nargs++; + XtSetArg(args[nargs], XtNorientation, XtOrientHorizontal); nargs++; + val_box = XtCreateManagedWidget("val_box", boxWidgetClass, form, args, nargs); + nargs = 0; + XtSetArg(args[nargs], XtNborderWidth, 0); nargs++; + XtSetArg(args[nargs], XtNlabel, " Value:"); nargs++; + (void) XtCreateManagedWidget("label", labelWidgetClass, val_box, args, nargs); + nargs = 0; + XtSetArg(args[nargs], XtNresizable, True); nargs++; + XtSetArg(args[nargs], XtNresize, XawtextResizeBoth); nargs++; + XtSetArg(args[nargs], XtNeditType, XawtextEdit); nargs++; + text = XtCreateManagedWidget("text",asciiTextWidgetClass, val_box, args, nargs); + my_translations(text); + nargs = 0; + XtSetArg(args[nargs], XtNmenuName, "tagMenu"); nargs++; + type = XtCreateManagedWidget("type",menuButtonWidgetClass, val_box, args, nargs); + createTagTypeMenu(type,tagMenuCallback); + /* Set default type */ + setButtonName(type, tag_db[0].type); + strncpy(tag_type,tag_db[0].id,4); + + /* + ** Create Exit Action Buttons + */ + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, val_box); nargs++; + XtSetArg(args[nargs], XtNborderWidth, 0); nargs++; + XtSetArg(args[nargs], XtNorientation, XtOrientHorizontal); nargs++; + act_box = XtCreateManagedWidget("act_box", boxWidgetClass, form, args, nargs); + nargs = 0; + XtSetArg(args[nargs], XtNlabel, "Search"); nargs++; + search = XtCreateManagedWidget("search", commandWidgetClass, act_box, args, nargs); + XtAddCallback(search, XtNcallback, searchCallback, (XtPointer) NULL); + nargs = 0; + XtSetArg(args[nargs], XtNlabel, "Quit"); nargs++; + quit = XtCreateManagedWidget("quit", commandWidgetClass, act_box, args, nargs); + XtAddCallback(quit, XtNcallback, quitCallback, (XtPointer) NULL); + +} + + + +int invokeSearchGeneric(EdStruct *xx) +/* +** Pop up the search window, if it isn't already up +*/ +{ + if (up) return 0; + XX = xx; + + if (searchWid == NULL) { + create_search_wid(oldFogieWid); + } + + XtPopup(searchWid, XtGrabNone); + + up = 1; + + return 0; +} + + + +int destroySearchWindow() +/* +** Shut this baby down +** Called by the routine that takes the contig editor down. +*/ +{ + if (up) + XtCallCallbacks(quit, XtNcallback, (XtPointer) NULL); + + return 0; +} + +void createSearchWidget(Widget parentWid) +/* +** Prtend to create it now +*/ +{ + oldFogieWid = parentWid; +} diff --git a/src/staden/searchUtils.h b/src/staden/searchUtils.h new file mode 100644 index 0000000..5cd52dd --- /dev/null +++ b/src/staden/searchUtils.h @@ -0,0 +1,24 @@ +#ifndef _searchUtils_h +#define _searchUtils_h + +#include +#include "edUtils.h" + + +enum searchOptions { + OptByGelName=1, + OptByTagType, + OptByAnnotation, + OptBySequence, + OptByPosition, + OptByProblem, + OptByQuality +}; + +extern void createSearchWidget(Widget parentWid); + +extern int invokeSearchGeneric(EdStruct *xx); + +extern int destroySearchWindow(); + +#endif /* _searchUtils_h */ diff --git a/src/staden/seeme-aix.f b/src/staden/seeme-aix.f new file mode 100644 index 0000000..17c8c0b --- /dev/null +++ b/src/staden/seeme-aix.f @@ -0,0 +1,684 @@ +C SEEME: MACHINE SPECIFIC ROUTINES +C 14-8-91 Added check in openrs for empty file names +C 27-8-91 Started files xspec.f and nxspec.f and split out the nonx +C and X specific bits from here. +C 27-8-91 Added FLUSHL to flush unit idev +C 15-10-91 Moved IANDRS, IORRS and WRITEB from pl4010.f +C 29-10-91 Replaced lnblnk ( a sun routine) by notrl +C 15-11-91 Added routines to call inquire to see if a file exists +C and another to delete a file +C 25-2-92 added more bit handling routines for use by sequence library +C searching routines +C 16-6-92 KH: added CALL NULLSB() in FNDFIL and SUBROUTINE NULLSB() for AIX; +C AUTHOR: RODGER STADEN +C 25-JAN-1990 INILU: SAVE DUP,PUP,DLOW,PLOW +C UNITNO +C OPENRS +C THE FOLLOWING ARE PROBABLY COMPLETELY PORTABLE +C BUT ARE INCLUDED ANY WAY +C INITLU +C IFROMP +C CTONUM +C DTONUM +C IUBM +C IUBM1 +C IUBM2 +C bsw4 +C bsw2 + SUBROUTINE UNITNO(KBIN,KBOUT,DEVNOS,NDEVS) +C AUTHOR: RODGER STADEN + INTEGER DEVNOS(NDEVS) +C SETS CONSOLE I/O UNITS TO 5,6. OTHERS 10 ONWARDS + KBIN=5 + KBOUT=6 + ISTART=10 + DO 1 I=1,NDEVS + DEVNOS(I)=ISTART+I-1 +1 CONTINUE + CALL INITRS() + END + SUBROUTINE OPENRS(IDEV,FN,IOK,LRECL,JOB) +C AUTHOR: RODGER STADEN + CHARACTER FN*(*) + CHARACTER*1024 FILNAM,FNDFIL + EXTERNAL FNDFIL,NOTRL,INQF +C 14-8-91 Added check for blank file names +C 14-11-91 Added routines to check for file existence +C ROUTINE TO OPEN FILES +C JOB=1 SEQUENTIAL FORMATTED NEW, DEFAULT RECORD LENGTH +C =2 SEQUENTIAL FORMATTED OLD, DEFAULT RECORD LENGTH +C =3 DIRECT ACCESS, UNFORMATTED NEW +C =4 DIRECT ACCESS, UNFORMATTED OLD +C =5 DIRECT ACCESS, UNFORMATTED OLD +C =6 UNFORMATTED NEW +C =7 UNFORMATTED OLD +C =8 STATUS='UNKNOWN' +C =9 TERMINAL FOR GRAPHICS +C =10 TERMINAL FOR VT100 +C =11 direct access, single byte record length, old +C RECORD LENGTHS SENT IN WORDS +C READONLY IS VAX SPECIFIC********************* +C NOTE THAT ONLY FOR DIRECT ACCESS FILES ARE RECORD +C LENGTHS SPECIFIED. ON THE VAX UNFORMATTED DIRECT ACCESS +C RECORDS ARE DEFINED IN WORDS; ON OTHER MACHINES E.G. SOME UNIX +C MACHINES THEY ARE SPECIFIED IN BYTES. THE VARIABLE IWORDL IS +C USED TO MULTIPLY RECORD LENGTHS SPECIFIED IN WORDS TO GIVE +C THE CORRECT VALUE FOR THE MACHINE. ON A VAX IWORDL=1; ON +C THE UNIX MACHINE IWORDL=4 + PARAMETER (IWORDL=4) + IF(NOTRL(FN,LEN(FN),' ').EQ.0) GO TO 100 + IF (JOB.LT.9 .OR. JOB.GT.10) THEN + FILNAM = FNDFIL(FN) + INQJ = INQF(FILNAM) + END IF + IF(JOB.EQ.1) THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + ACCESS='SEQUENTIAL',ERR=100) + ELSE IF(JOB.EQ.2)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='SEQUENTIAL',ERR=100) + ELSE IF(JOB.EQ.3)THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.4)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.5)THEN + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.6)THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + FORM='UNFORMATTED',ERR=100) + ELSE IF(JOB.EQ.7)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + FORM='UNFORMATTED',ERR=100) + ELSE IF(JOB.EQ.8)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.9)THEN +C DEVICE FOR GRAPHICS OUTPUT IS 'TT' IF USING A +C VT640 ON A VAX. IF USING A SEPARATE TERMINAL FOR +C GRAPHICS OUTPUT THE TERMINAL PROTECTION MUST BE +C SET W:RW AND THE DEVICE WILL HAVE A DIFFERENT NAME +C FOR EXAMPLE 'GRAPHICS' WHERE THE LOGICAL NAME 'GRAPHICS' +C IS ASSIGNED TO THE APPROPRIATE DEVICE. A VT240 SHOULD +C BE COMPATIBLE WITH THE TEKTRONIX PLOT COMMANDS. + OPEN(UNIT=IDEV,FILE='/dev/tty',STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.10)THEN +C DEVICE FOR VT100 COMPATIBLE TERMINAL WHEN USED +C FOR GELIN WHICH REQUIRES COMPLETE CONTROL OF +C CURSOR POSITIONING. ON A VAX IT IS CALLED 'TT' + OPEN(UNIT=IDEV,FILE='/dev/tty',STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.11)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + JRECL=1 + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + END IF + IOK=0 + RETURN +100 CONTINUE + IOK=1 + RETURN + END + CHARACTER*1024 FUNCTION FNDFIL(NAME) + CHARACTER NAME*(*),TMPNAM*1024,PREFIX*1024 + EXTERNAL NOTRL +C LOOKS IN ENVIRONMENT FOR FILE PATHS +C ? CASES DEALT WITH: +C 1) IF NAME STARTS WITH / IT IS ABSOLUTE AND SO UNCHANGED +C 2) IF NAME STARTS WITH %/ REPLACE WITH HOME DIRECTORY NAME +C 3) IF NAME INCLUDES ANY OTHER / LOOK IN ENVIRONMENT FOR +C NAME UPTO /, AND ADD PATH TO REST IF FOUND +C IF NOT FOUND ADD PATH TO CURRENT WORKING DIRECTORY +C 4) ELSE LOOK IN ENVIRONMENT FOR WHOLE NAME +C IF NOT FOUND ADD PATH TO CURRENT DIRECTORY +C IF FOUND MAKE IT FILE NAME +C + call NULLSB(NAME) + IF(NAME(1:1).EQ.'/') THEN + FNDFIL = NAME + ELSE IF (NAME(1:2).EQ.'%/') THEN + CALL GETENV('HOME',TMPNAM) + CALL NULLSB(TMPNAM) + FNDFIL = TMPNAM(:NOTRL(TMPNAM,LEN(TMPNAM),' ')) // + + NAME(2:NOTRL(NAME,LEN(NAME),' ')) + ELSE IF (INDEX(NAME,'/').NE.0) THEN + TMPNAM = NAME(1:INDEX(NAME,'/')-1) + CALL GETENV(TMPNAM,PREFIX) + CALL NULLSB(PREFIX) + I = NOTRL(PREFIX,LEN(PREFIX),' ') + IF(I.EQ.0) THEN + CALL GETCWD(PREFIX) + call NULLSB(PREFIX) + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + '/' // NAME(:NOTRL(NAME,LEN(NAME),' ')) + ELSE + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + NAME(INDEX(NAME,'/'):) + END IF + ELSE + CALL GETENV(NAME,TMPNAM) + CALL NULLSB(TMPNAM) + I = NOTRL(TMPNAM,LEN(TMPNAM),' ') + IF (I.EQ.0) THEN + CALL GETCWD(PREFIX) + CALL NULLSB(PREFIX) + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + '/' // NAME(:NOTRL(NAME,LEN(NAME),' ')) + ELSE + FNDFIL = TMPNAM + END IF + END IF + END + SUBROUTINE NULLSB(STRING) +C +C KH: simply convert all char(0) to blanks in a given string +C + CHARACTER STRING*(*), Z*1 + Z=CHAR(0) + DO K=1, LEN(STRING) + IF (STRING(K:K) .EQ. Z) STRING(K:K)=' ' + END DO + RETURN + END + INTEGER FUNCTION INQF(NAME) + CHARACTER NAME*(*) + LOGICAL EX + INQUIRE(FILE=NAME,EXIST=EX) +C +C if the file exists return 1, else 0 +C + IF (EX) THEN + INQF = 1 + ELSE + INQF = 0 + END IF + END + INTEGER FUNCTION DELF(FN,IDEV,JRECL,JOB) + CHARACTER FN*(*) + CHARACTER FILNAM*1024,FNDFIL*1024 + EXTERNAL FNDFIL +C +C routine to delete files in the most horrible way i could find +C deal with job 2: old sequential formatted +C deal with job 4: old direct unformatted +C deal with job 7: old sequential unformatted +C if the file exists and is not one of these error =-1 +C if the file exists and cannot be deleted error =-2 +C if the file cannot be opened error =-3 +C +C on the sun this technique will delete any file if the directory +C priviliges are set. +C I dont know if i need to worry about the file types: could +C i just open them all the same way? Although its a mess leave it! + FILNAM = FNDFIL(FN) + IF(JOB.EQ.2) THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='SEQUENTIAL',ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE IF(JOB.EQ.4)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE IF(JOB.EQ.7)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + FORM='UNFORMATTED',ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE + DELF = -1 + RETURN + END IF + RETURN + 100 CONTINUE + DELF = -3 + RETURN + 200 CONTINUE + DELF = -2 + END +C ROUTINES TO CONTROL CHARACTER LOOKUP +C FOR BOTH DNA AND PROTEIN SEQUENCES +C THE INITIALISING ROUTINES ARE SENT THE CHARACTERSET SIZE IDM +C WHICH DETERMINES WHICH CHARACTERSET IS USED + SUBROUTINE INITLU(IDM) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255),POINT2(0:255) + CHARACTER DUP*16,DLOW*16,PUP*26,PLOW*26 + COMMON /IASCI1/POINT1 + COMMON /IASCI2/POINT2 + SAVE /IASCI1/ + SAVE /IASCI2/ + SAVE DUP,PUP,DLOW,PLOW + DATA DUP/'TCAG-RYWSMKHBVDN'/ + DATA PUP/'CSTPAGNDEQBZHRKMILVFYW-X? '/ + DATA DLOW/'tcag-rywsmkhbvdn'/ + DATA PLOW/'cstpagndeqbzhrkmilvfyw-x? '/ +C ICHAR RETURNS THE COLLATING SEQUENCE NUMBER +C I WANT 1-5 FOR ACGT OR 1-26 FOR AMINO ACIDS BY USING ICHAR. +C THE ACTUAL VALUE RETURNED BY ICHAR IS NOT PORTABLE +C SO I NEED TO INITIALIZE POINTR SO THAT THE CORRECT +C ELEMENTS CONTAIN VALUES 1 - 5, OR 1 - 26 +C WORKS ON UPPER AND LOWER CASE - REMOVE DLOW,PLOW AND LOOPS 41 AND 51 +C IF LOWERCASE NOT ALLOWED +C + IF(IDM.EQ.5)THEN + DO 30 I = 0,255 + POINT1(I) = IDM + POINT2(I) = 17 +30 CONTINUE + DO 35 I = 1,5 + J = ICHAR(DUP(I:I)) + POINT1(J) = I +35 CONTINUE + DO 36 I = 1,5 + J = ICHAR(DLOW(I:I)) + POINT1(J) = I +36 CONTINUE + DO 40 I = 1,16 + J = ICHAR(DUP(I:I)) + POINT2(J) = I +40 CONTINUE +C DEAL WITH U + J = ICHAR('U') + POINT1(J) = 1 + POINT2(J) = 1 + DO 41 I = 1,16 + J = ICHAR(DLOW(I:I)) + POINT2(J) = I +41 CONTINUE +C DEAL WITH U + J = ICHAR('u') + POINT1(J) = 1 + POINT2(J) = 1 + ELSE IF(IDM.EQ.26)THEN + DO 45 I = 0,255 + POINT1(I) = IDM +45 CONTINUE +C + DO 50 I = 1,26 + J = ICHAR(PUP(I:I)) + POINT1(J) = I +50 CONTINUE + DO 51 I = 1,26 + J = ICHAR(PLOW(I:I)) + POINT1(J) = I +51 CONTINUE + DO 60 I = 0,255 + POINT2(I) = POINT1(I) +60 CONTINUE + ELSE + WRITE(*,*)'ERROR INITIALISING CHARACTER LOOKUP POINTERS' + END IF + END + INTEGER FUNCTION IFROMP(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255) + CHARACTER CHAR + COMMON /IASCI1/POINT1 + SAVE /IASCI1/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + IFROMP = POINT1(ICOL) + END + INTEGER FUNCTION CTONUM(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255) + CHARACTER CHAR + COMMON /IASCI1/POINT1 + SAVE /IASCI1/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + CTONUM = POINT1(ICOL) + END + INTEGER FUNCTION DTONUM(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT2(0:255) + CHARACTER CHAR + COMMON /IASCI2/POINT2 + SAVE /IASCI2/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + DTONUM = POINT2(ICOL) + END + INTEGER FUNCTION IUBM(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A DEFINITE MATCH, 2 FOR POSSIBLE, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,2,0,2,2,0,0,2,2,2,0,2,2,0, + +0,1,0,0,2,0,2,0,2,2,0,2,2,2,0,2,0, + +0,0,1,0,2,2,0,2,0,2,0,2,0,2,2,2,0, + +0,0,0,1,2,2,0,0,2,0,2,0,2,2,2,2,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,2,1,0,2,2,2,2,2,2,2,2,2,0, + +1,1,0,0,2,0,1,2,2,2,2,2,2,2,2,2,0, + +1,0,1,0,2,2,2,1,0,2,2,2,2,2,2,2,0, + +0,1,0,1,2,2,2,0,1,2,2,2,2,2,2,2,0, + +0,1,1,0,2,2,2,2,2,1,0,2,2,2,2,2,0, + +1,0,0,1,2,2,2,2,2,2,1,2,2,2,2,2,0, + +1,1,1,0,2,2,2,1,2,1,2,1,2,2,2,2,0, + +1,1,0,1,2,2,1,2,1,2,1,2,1,2,2,2,0, + +0,1,1,1,2,1,2,2,1,1,2,2,2,1,2,2,0, + +1,0,1,1,2,1,2,1,2,2,1,2,2,2,1,2,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END + INTEGER FUNCTION IUBM2(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A POSSIBLE MATCH, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,1,0,1,1,0,0,1,1,1,0,1,1,0, + +0,1,0,0,1,0,1,0,1,1,0,1,1,1,0,1,0, + +0,0,1,0,1,1,0,1,0,1,0,1,0,1,1,1,0, + +0,0,0,1,1,1,0,0,1,0,1,0,1,1,1,1,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,1,1,0,1,1,1,1,1,1,1,1,1,0, + +1,1,0,0,1,0,1,1,1,1,1,1,1,1,1,1,0, + +1,0,1,0,1,1,1,1,0,1,1,1,1,1,1,1,0, + +0,1,0,1,1,1,1,0,1,1,1,1,1,1,1,1,0, + +0,1,1,0,1,1,1,1,1,1,0,1,1,1,1,1,0, + +1,0,0,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,1,0,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,0,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +0,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,0,1,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM2 = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END + INTEGER FUNCTION IUBM1(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A DEFINITE MATCH, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,0,1,0,0,0,0,0,0,0,0,0,0,0, + +1,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0,0, + +1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0, + +0,1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0, + +0,1,1,0,0,0,0,0,0,1,0,0,0,0,0,0,0, + +1,0,0,1,0,0,0,0,0,0,1,0,0,0,0,0,0, + +1,1,1,0,0,0,0,1,0,1,0,1,0,0,0,0,0, + +1,1,0,1,0,0,1,0,1,0,1,0,1,0,0,0,0, + +0,1,1,1,0,1,0,0,1,1,0,0,0,1,0,0,0, + +1,0,1,1,0,1,0,1,0,0,1,0,0,0,1,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM1 = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END +C +C + SUBROUTINE FLUSHL(IDEV) + CALL FLUSH(IDEV) +C This implementation uses the Sun Fortran FLUSH statement. + END + SUBROUTINE FLUSHO +C The (single) unit number is currently hard-wired, but must +C be the same as KBOUT in UNITNO. + CALL FLUSHL(6) + END +C + SUBROUTINE CEDIT(FILNAM) + CHARACTER COMAND*256,EDITOR*256,FILNAM*(*) + INTEGER SYSTEM,I + EXTERNAL NOTRL + CALL GETENV('SEQEDT',EDITOR) + I=NOTRL(EDITOR,LEN(EDITOR),' ') + IF (I.EQ.0) THEN + EDITOR='vi' + I=2 + ENDIF + COMAND = EDITOR(1:I)//' '//FILNAM + ISTAT = SYSTEM(COMAND) + END + SUBROUTINE BSW2(IN,OUT) +C byte order on cd is least significant first +C on some machines this routine should be changed to do nothing + CHARACTER*2 IN,OUT +C Sun + OUT(1:1) = IN(2:2) + OUT(2:2) = IN(1:2) +C Alliant +C OUT = IN + END + SUBROUTINE BSW4(IN,OUT) +C byte order on cd is least significant first +C on some machines this routine should be changed to do nothing + CHARACTER IN*4,OUT*4 +C Sun + DO 10 I = 1,2 + OUT(I:I) = IN(5-I:5-I) + OUT(5-I:5-I) = IN(I:I) + 10 CONTINUE +C Alliant +C OUT = IN + END + INTEGER FUNCTION IANDRS(IIN,JIN) +C AUTHOR: RODGER STADEN +C LOGICAL LIIN,LJIN +C INTEGER IN,JN +C EQUIVALENCE (LIIN,IN),(LJIN,JN) +******* PERFORMS LOGICAL OPERATIONS ON INTEGER VARIABLES +******* SO COULD BE FLAGGED AS ILLEGAL OR GIVE INCORRECT RESULTS +******* ON MANY MACHINES CAN BE REPLACED BY LOCAL INTRINSIC IAND +C IN=IIN +C JN=JIN +C LIIN=LIIN.AND.LJIN +C Sun + IANDRS=AND(IIN,JIN) +C Alliant +C IANDRS=IAND(IIN,JIN) + END + INTEGER FUNCTION IORRS(IIN,JIN) +C AUTHOR: RODGER STADEN +C LOGICAL LIIN,LJIN +C INTEGER IN,JN +C EQUIVALENCE (LIIN,IN),(LJIN,JN) +******* PERFORMS LOGICAL OPERATIONS ON INTEGER VARIABLES +******* SO COULD BE FLAGGED AS ILLEGAL OR GIVE INCORRECT RESULTS +******* ON MANY MACHINES CAN BE REPLACED BY LOCAL INTRINSIC IOR +C IN=IIN +C JN=JIN +C LIIN=LIIN.OR.LJIN +C Sun + IORRS=OR(IIN,JIN) +C Alliant +C IORRS=IOR(IIN,JIN) + END + SUBROUTINE WRITEB(CHARS,NCHAR,IDEVGR) +C AUTHOR: RODGER STADEN + CHARACTER CHARS*(*) + SAVE NUL + DATA NUL/0/ +C NOTE NULS STOP CARRIAGE RETURN, LINE FEED +C Sun + WRITE(IDEVGR,1000)CHARS(1:NCHAR) +1000 FORMAT(A,$) +C Alliant +C WRITE(IDEVGR,1000)NUL,CHARS(1:NCHAR) +C1000 FORMAT(A1,A) + END + SUBROUTINE SABIT(ARRAY,MAXAR,POSN) + INTEGER ARRAY(0:MAXAR),POSN,ELEMNT,BITNUM +C +C set bit corresponding to posn in array (bits 0-31 per word) +C + I = POSN - 1 + ELEMNT = I / 32 + BITNUM = MOD(I,32) +C WRITE(*,*)'ELEMENT, BIT',ELEMNT,BITNUM + CALL SBITRS(ARRAY(ELEMNT),BITNUM) + END + SUBROUTINE AAWORD(ARRAY1,ARRAY2,MAXAR) + INTEGER ARRAY1(0:MAXAR),ARRAY2(0:MAXAR) + INTEGER IANDRS + EXTERNAL IANDRS +C +C and array1 and array2 and put result in array1 +C + DO 10 I=0,MAXAR + ARRAY1(I) = IANDRS(ARRAY1(I),ARRAY2(I)) + 10 CONTINUE + END + SUBROUTINE OAWORD(ARRAY1,ARRAY2,MAXAR) + INTEGER ARRAY1(0:MAXAR),ARRAY2(0:MAXAR) + INTEGER IORRS + EXTERNAL IORRS +C +C OR array1 and array2 and put result in array1 +C + DO 10 I=0,MAXAR + ARRAY1(I) = IORRS(ARRAY1(I),ARRAY2(I)) + 10 CONTINUE + END + LOGICAL FUNCTION TABIT(ARRAY,MAXAR,POSN) + INTEGER ARRAY(0:MAXAR),POSN,ELEMNT,BIT + LOGICAL TBITRS + EXTERNAL TBITRS +C +C test bit corresponding to posn in array (bits 0-31 per word) +C + I = POSN - 1 + ELEMNT = I / 32 + BIT = MOD(I,32) +C WRITE(*,*)'TEST ELEMENT, BIT',ELEMNT,BIT + TABIT = TBITRS(ARRAY(ELEMNT),BIT) + END + SUBROUTINE CABIT(ARRAY,MAXAR) + INTEGER ARRAY(0:MAXAR),POSN +C +C clear all bits in array (1 at a time!!!!!!!!) +C + DO 10 POSN=0,MAXAR + DO 5 J = 0,31 + CALL CBITRS(ARRAY(POSN),J) + 5 CONTINUE + 10 CONTINUE + END + SUBROUTINE TBITA(ARRAY,MAXAR,IDEV) + INTEGER ARRAY(0:MAXAR) + LOGICAL TBITRS + EXTERNAL TBITRS +C +C test all bits in array to see if they are set +C + DO 10 I=0,MAXAR + DO 5 J=0,31 + IF(TBITRS(ARRAY(I),J)) THEN + K = I*32 + 1 + MOD(J,32) + WRITE(IDEV,*)' BIT SET FOR POSN',K + END IF + 5 CONTINUE + 10 CONTINUE + END +C routines to handle bits (machine specific) for use when +C searching through keyword indexes +C +C positions for marking are numbered from 1 onwards +C but array elements and bits are numbered from 0 onwards +C + SUBROUTINE SBITRS(WORD,BITNUM) +C +C set bit bitnum in word +C + INTEGER WORD,BITNUM +C alliant & AIX + INTEGER IBSET + INTRINSIC IBSET + WORD = IBSET(WORD,BITNUM) +C sun +C CALL BIS(BITNUM,WORD) + END + SUBROUTINE CBITRS(WORD,BITNUM) +C +C clear bit bitnum in word +C + INTEGER WORD,BITNUM +C alliant & AIX + INTEGER IBCLR + INTRINSIC IBCLR + WORD = IBCLR(WORD,BITNUM) +C sun +C CALL BIC(BITNUM,WORD) + END + LOGICAL FUNCTION TBITRS(WORD,BITNUM) +C +C test bit bitnum in word +C + INTEGER WORD,BITNUM +C alliant & AIX + LOGICAL BTEST + INTRINSIC BTEST + TBITRS = BTEST(WORD,BITNUM) +C sun +C LOGICAL BIT +C EXTERNAL BIT +C TBITRS = BIT(BITNUM,WORD) + END diff --git a/src/staden/seeme-al.f b/src/staden/seeme-al.f new file mode 100644 index 0000000..c940ee6 --- /dev/null +++ b/src/staden/seeme-al.f @@ -0,0 +1,715 @@ +C SEEME: MACHINE SPECIFIC ROUTINES +C 11-12-92 fseek fix for al new bug +C 14-8-91 Added check in openrs for empty file names +C 27-8-91 Started files xspec.f and nxspec.f and split out the nonx +C and X specific bits from here. +C 27-8-91 Added FLUSHL to flush unit idev +C 15-10-91 Moved IANDRS, IORRS and WRITEB from pl4010.f +C 29-10-91 Replaced lnblnk ( a sun routine) by notrl +C 15-11-91 Added routines to call inquire to see if a file exists +C and another to delete a file +C 25-2-92 added more bit handling routines for use by sequence library +C searching routines +C 20-5-92 moved getrs, getrsl and seekrs to here from seqlibsubs.f +C AUTHOR: RODGER STADEN +C 25-JAN-1990 INILU: SAVE DUP,PUP,DLOW,PLOW +C UNITNO +C OPENRS +C THE FOLLOWING ARE PROBABLY COMPLETELY PORTABLE +C BUT ARE INCLUDED ANY WAY +C INITLU +C IFROMP +C CTONUM +C DTONUM +C IUBM +C IUBM1 +C IUBM2 +C bsw4 +C bsw2 + SUBROUTINE UNITNO(KBIN,KBOUT,DEVNOS,NDEVS) +C AUTHOR: RODGER STADEN + INTEGER DEVNOS(NDEVS) +C SETS CONSOLE I/O UNITS TO 5,6. OTHERS 10 ONWARDS + KBIN=5 + KBOUT=6 + ISTART=10 + DO 1 I=1,NDEVS + DEVNOS(I)=ISTART+I-1 +1 CONTINUE + CALL INITRS() + END + SUBROUTINE OPENRS(IDEV,FN,IOK,LRECL,JOB) +C AUTHOR: RODGER STADEN + CHARACTER FN*(*) + CHARACTER*1024 FILNAM,FNDFIL + EXTERNAL FNDFIL,NOTRL,INQF +C 14-8-91 Added check for blank file names +C 14-11-91 Added routines to check for file existence +C ROUTINE TO OPEN FILES +C JOB=1 SEQUENTIAL FORMATTED NEW, DEFAULT RECORD LENGTH +C =2 SEQUENTIAL FORMATTED OLD, DEFAULT RECORD LENGTH +C =3 DIRECT ACCESS, UNFORMATTED NEW +C =4 DIRECT ACCESS, UNFORMATTED OLD +C =5 DIRECT ACCESS, UNFORMATTED OLD +C =6 UNFORMATTED NEW +C =7 UNFORMATTED OLD +C =8 STATUS='UNKNOWN' +C =9 TERMINAL FOR GRAPHICS +C =10 TERMINAL FOR VT100 +C =11 direct access, single byte record length, old +C RECORD LENGTHS SENT IN WORDS +C READONLY IS VAX SPECIFIC********************* +C NOTE THAT ONLY FOR DIRECT ACCESS FILES ARE RECORD +C LENGTHS SPECIFIED. ON THE VAX UNFORMATTED DIRECT ACCESS +C RECORDS ARE DEFINED IN WORDS; ON OTHER MACHINES E.G. SOME UNIX +C MACHINES THEY ARE SPECIFIED IN BYTES. THE VARIABLE IWORDL IS +C USED TO MULTIPLY RECORD LENGTHS SPECIFIED IN WORDS TO GIVE +C THE CORRECT VALUE FOR THE MACHINE. ON A VAX IWORDL=1; ON +C THE UNIX MACHINE IWORDL=4 + PARAMETER (IWORDL=4) + IF(NOTRL(FN,LEN(FN),' ').EQ.0) GO TO 100 + IF (JOB.LT.9 .OR. JOB.GT.10) THEN + FILNAM = FNDFIL(FN) + INQJ = INQF(FILNAM) + END IF + IF(JOB.EQ.1) THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + ACCESS='SEQUENTIAL',ERR=100) + ELSE IF(JOB.EQ.2)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='SEQUENTIAL',ERR=100) + ELSE IF(JOB.EQ.3)THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.4)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.5)THEN + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.6)THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + FORM='UNFORMATTED',ERR=100) + ELSE IF(JOB.EQ.7)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + FORM='UNFORMATTED',ERR=100) + ELSE IF(JOB.EQ.8)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.9)THEN +C DEVICE FOR GRAPHICS OUTPUT IS 'TT' IF USING A +C VT640 ON A VAX. IF USING A SEPARATE TERMINAL FOR +C GRAPHICS OUTPUT THE TERMINAL PROTECTION MUST BE +C SET W:RW AND THE DEVICE WILL HAVE A DIFFERENT NAME +C FOR EXAMPLE 'GRAPHICS' WHERE THE LOGICAL NAME 'GRAPHICS' +C IS ASSIGNED TO THE APPROPRIATE DEVICE. A VT240 SHOULD +C BE COMPATIBLE WITH THE TEKTRONIX PLOT COMMANDS. + OPEN(UNIT=IDEV,FILE='/dev/tty',STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.10)THEN +C DEVICE FOR VT100 COMPATIBLE TERMINAL WHEN USED +C FOR GELIN WHICH REQUIRES COMPLETE CONTROL OF +C CURSOR POSITIONING. ON A VAX IT IS CALLED 'TT' + OPEN(UNIT=IDEV,FILE='/dev/tty',STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.11)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + JRECL=1 + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + END IF + IOK=0 + RETURN +100 CONTINUE + IOK=1 + RETURN + END + CHARACTER*1024 FUNCTION FNDFIL(NAME) + CHARACTER NAME*(*),TMPNAM*1024,PREFIX*1024 + EXTERNAL NOTRL +C LOOKS IN ENVIRONMENT FOR FILE PATHS +C ? CASES DEALT WITH: +C 1) IF NAME STARTS WITH / IT IS ABSOLUTE AND SO UNCHANGED +C 2) IF NAME STARTS WITH ~/ REPLACE WITH HOME DIRECTORY NAME +C 3) IF NAME INCLUDES ANY OTHER / LOOK IN ENVIRONMENT FOR +C NAME UPTO /, AND ADD PATH TO REST IF FOUND +C IF NOT FOUND ADD PATH TO CURRENT WORKING DIRECTORY +C 4) ELSE LOOK IN ENVIRONMENT FOR WHOLE NAME +C IF NOT FOUND ADD PATH TO CURRENT DIRECTORY +C IF FOUND MAKE IT FILE NAME +C + IF(NAME(1:1).EQ.'/') THEN + FNDFIL = NAME + ELSE IF (NAME(1:2).EQ.'~/') THEN + CALL GETENV('HOME',TMPNAM) + FNDFIL = TMPNAM(:NOTRL(TMPNAM,LEN(TMPNAM),' ')) // + + NAME(2:NOTRL(NAME,LEN(NAME),' ')) + ELSE IF (INDEX(NAME,'/').NE.0) THEN + TMPNAM = NAME(1:INDEX(NAME,'/')-1) + CALL GETENV(TMPNAM,PREFIX) + I = NOTRL(PREFIX,LEN(PREFIX),' ') + IF(I.EQ.0) THEN + CALL GETCWD(PREFIX) + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + '/' // NAME(:NOTRL(NAME,LEN(NAME),' ')) + ELSE + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + NAME(INDEX(NAME,'/'):) + END IF + ELSE + CALL GETENV(NAME,TMPNAM) + I = NOTRL(TMPNAM,LEN(TMPNAM),' ') + IF (I.EQ.0) THEN + CALL GETCWD(PREFIX) + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + '/' // NAME(:NOTRL(NAME,LEN(NAME),' ')) + ELSE + FNDFIL = TMPNAM + END IF + END IF + END + INTEGER FUNCTION INQF(NAME) + CHARACTER NAME*(*) + LOGICAL EX + INQUIRE(FILE=NAME,EXIST=EX) +C +C if the file exists return 1, else 0 +C + IF (EX) THEN + INQF = 1 + ELSE + INQF = 0 + END IF + END + INTEGER FUNCTION DELF(FN,IDEV,JRECL,JOB) + CHARACTER FN*(*) + CHARACTER FILNAM*1024,FNDFIL*1024 + EXTERNAL FNDFIL +C +C routine to delete files in the most horrible way i could find +C deal with job 2: old sequential formatted +C deal with job 4: old direct unformatted +C deal with job 7: old sequential unformatted +C if the file exists and is not one of these error =-1 +C if the file exists and cannot be deleted error =-2 +C if the file cannot be opened error =-3 +C +C on the sun this technique will delete any file if the directory +C priviliges are set. +C I dont know if i need to worry about the file types: could +C i just open them all the same way? Although its a mess leave it! + FILNAM = FNDFIL(FN) + IF(JOB.EQ.2) THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='SEQUENTIAL',ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE IF(JOB.EQ.4)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE IF(JOB.EQ.7)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + FORM='UNFORMATTED',ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE + DELF = -1 + RETURN + END IF + RETURN + 100 CONTINUE + DELF = -3 + RETURN + 200 CONTINUE + DELF = -2 + END +C ROUTINES TO CONTROL CHARACTER LOOKUP +C FOR BOTH DNA AND PROTEIN SEQUENCES +C THE INITIALISING ROUTINES ARE SENT THE CHARACTERSET SIZE IDM +C WHICH DETERMINES WHICH CHARACTERSET IS USED + SUBROUTINE INITLU(IDM) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255),POINT2(0:255) + CHARACTER DUP*16,DLOW*16,PUP*26,PLOW*26 + COMMON /IASCI1/POINT1 + COMMON /IASCI2/POINT2 + SAVE /IASCI1/ + SAVE /IASCI2/ + SAVE DUP,PUP,DLOW,PLOW + DATA DUP/'TCAG-RYWSMKHBVDN'/ + DATA PUP/'CSTPAGNDEQBZHRKMILVFYW-X? '/ + DATA DLOW/'tcag-rywsmkhbvdn'/ + DATA PLOW/'cstpagndeqbzhrkmilvfyw-x? '/ +C ICHAR RETURNS THE COLLATING SEQUENCE NUMBER +C I WANT 1-5 FOR ACGT OR 1-26 FOR AMINO ACIDS BY USING ICHAR. +C THE ACTUAL VALUE RETURNED BY ICHAR IS NOT PORTABLE +C SO I NEED TO INITIALIZE POINTR SO THAT THE CORRECT +C ELEMENTS CONTAIN VALUES 1 - 5, OR 1 - 26 +C WORKS ON UPPER AND LOWER CASE - REMOVE DLOW,PLOW AND LOOPS 41 AND 51 +C IF LOWERCASE NOT ALLOWED +C + IF(IDM.EQ.5)THEN + DO 30 I = 0,255 + POINT1(I) = IDM + POINT2(I) = 17 +30 CONTINUE + DO 35 I = 1,5 + J = ICHAR(DUP(I:I)) + POINT1(J) = I +35 CONTINUE + DO 36 I = 1,5 + J = ICHAR(DLOW(I:I)) + POINT1(J) = I +36 CONTINUE + DO 40 I = 1,16 + J = ICHAR(DUP(I:I)) + POINT2(J) = I +40 CONTINUE +C DEAL WITH U + J = ICHAR('U') + POINT1(J) = 1 + POINT2(J) = 1 + DO 41 I = 1,16 + J = ICHAR(DLOW(I:I)) + POINT2(J) = I +41 CONTINUE +C DEAL WITH U + J = ICHAR('u') + POINT1(J) = 1 + POINT2(J) = 1 + ELSE IF(IDM.EQ.26)THEN + DO 45 I = 0,255 + POINT1(I) = IDM +45 CONTINUE +C + DO 50 I = 1,26 + J = ICHAR(PUP(I:I)) + POINT1(J) = I +50 CONTINUE + DO 51 I = 1,26 + J = ICHAR(PLOW(I:I)) + POINT1(J) = I +51 CONTINUE + DO 60 I = 0,255 + POINT2(I) = POINT1(I) +60 CONTINUE + ELSE + WRITE(*,*)'ERROR INITIALISING CHARACTER LOOKUP POINTERS' + END IF + END + INTEGER FUNCTION IFROMP(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255) + CHARACTER CHAR + COMMON /IASCI1/POINT1 + SAVE /IASCI1/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + IFROMP = POINT1(ICOL) + END + INTEGER FUNCTION CTONUM(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255) + CHARACTER CHAR + COMMON /IASCI1/POINT1 + SAVE /IASCI1/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + CTONUM = POINT1(ICOL) + END + INTEGER FUNCTION DTONUM(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT2(0:255) + CHARACTER CHAR + COMMON /IASCI2/POINT2 + SAVE /IASCI2/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + DTONUM = POINT2(ICOL) + END + INTEGER FUNCTION IUBM(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A DEFINITE MATCH, 2 FOR POSSIBLE, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,2,0,2,2,0,0,2,2,2,0,2,2,0, + +0,1,0,0,2,0,2,0,2,2,0,2,2,2,0,2,0, + +0,0,1,0,2,2,0,2,0,2,0,2,0,2,2,2,0, + +0,0,0,1,2,2,0,0,2,0,2,0,2,2,2,2,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,2,1,0,2,2,2,2,2,2,2,2,2,0, + +1,1,0,0,2,0,1,2,2,2,2,2,2,2,2,2,0, + +1,0,1,0,2,2,2,1,0,2,2,2,2,2,2,2,0, + +0,1,0,1,2,2,2,0,1,2,2,2,2,2,2,2,0, + +0,1,1,0,2,2,2,2,2,1,0,2,2,2,2,2,0, + +1,0,0,1,2,2,2,2,2,2,1,2,2,2,2,2,0, + +1,1,1,0,2,2,2,1,2,1,2,1,2,2,2,2,0, + +1,1,0,1,2,2,1,2,1,2,1,2,1,2,2,2,0, + +0,1,1,1,2,1,2,2,1,1,2,2,2,1,2,2,0, + +1,0,1,1,2,1,2,1,2,2,1,2,2,2,1,2,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END + INTEGER FUNCTION IUBM2(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A POSSIBLE MATCH, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,1,0,1,1,0,0,1,1,1,0,1,1,0, + +0,1,0,0,1,0,1,0,1,1,0,1,1,1,0,1,0, + +0,0,1,0,1,1,0,1,0,1,0,1,0,1,1,1,0, + +0,0,0,1,1,1,0,0,1,0,1,0,1,1,1,1,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,1,1,0,1,1,1,1,1,1,1,1,1,0, + +1,1,0,0,1,0,1,1,1,1,1,1,1,1,1,1,0, + +1,0,1,0,1,1,1,1,0,1,1,1,1,1,1,1,0, + +0,1,0,1,1,1,1,0,1,1,1,1,1,1,1,1,0, + +0,1,1,0,1,1,1,1,1,1,0,1,1,1,1,1,0, + +1,0,0,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,1,0,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,0,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +0,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,0,1,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM2 = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END + INTEGER FUNCTION IUBM1(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A DEFINITE MATCH, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,0,1,0,0,0,0,0,0,0,0,0,0,0, + +1,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0,0, + +1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0, + +0,1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0, + +0,1,1,0,0,0,0,0,0,1,0,0,0,0,0,0,0, + +1,0,0,1,0,0,0,0,0,0,1,0,0,0,0,0,0, + +1,1,1,0,0,0,0,1,0,1,0,1,0,0,0,0,0, + +1,1,0,1,0,0,1,0,1,0,1,0,1,0,0,0,0, + +0,1,1,1,0,1,0,0,1,1,0,0,0,1,0,0,0, + +1,0,1,1,0,1,0,1,0,0,1,0,0,0,1,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM1 = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END +C +C + SUBROUTINE FLUSHL(IDEV) + CALL FLUSH(IDEV) +C This implementation uses the Sun Fortran FLUSH statement. + END + SUBROUTINE FLUSHO +C The (single) unit number is currently hard-wired, but must +C be the same as KBOUT in UNITNO. + CALL FLUSHL(6) + END +C + SUBROUTINE CEDIT(FILNAM) + CHARACTER COMAND*256,EDITOR*256,FILNAM*(*) + INTEGER SYSTEM,I + EXTERNAL NOTRL + CALL GETENV('SEQEDT',EDITOR) + I=NOTRL(EDITOR,LEN(EDITOR),' ') + IF (I.EQ.0) THEN + EDITOR='vi' + I=2 + ENDIF + COMAND = EDITOR(1:I)//' '//FILNAM + ISTAT = SYSTEM(COMAND) + END + SUBROUTINE BSW2(IN,OUT) +C byte order on cd is least significant first +C on some machines this routine should be changed to do nothing + CHARACTER*2 IN,OUT +C Sun +C OUT(1:1) = IN(2:2) +C OUT(2:2) = IN(1:2) +C Alliant + OUT = IN + END + SUBROUTINE BSW4(IN,OUT) +C byte order on cd is least significant first +C on some machines this routine should be changed to do nothing + CHARACTER IN*4,OUT*4 +C Sun +C DO 10 I = 1,2 +C OUT(I:I) = IN(5-I:5-I) +C OUT(5-I:5-I) = IN(I:I) +C 10 CONTINUE +C Alliant + OUT = IN + END + INTEGER FUNCTION IANDRS(IIN,JIN) +C AUTHOR: RODGER STADEN +C LOGICAL LIIN,LJIN + INTEGER IN,JN +C EQUIVALENCE (LIIN,IN),(LJIN,JN) +******* PERFORMS LOGICAL OPERATIONS ON INTEGER VARIABLES +******* SO COULD BE FLAGGED AS ILLEGAL OR GIVE INCORRECT RESULTS +******* ON MANY MACHINES CAN BE REPLACED BY LOCAL INTRINSIC IAND +C IN=IIN +C JN=JIN +C LIIN=LIIN.AND.LJIN +C Sun +C IANDRS=AND(IIN,JIN) +C Alliant + IANDRS=IAND(IIN,JIN) + RETURN + END + INTEGER FUNCTION IORRS(IIN,JIN) +C AUTHOR: RODGER STADEN +C LOGICAL LIIN,LJIN +C INTEGER IN,JN +C EQUIVALENCE (LIIN,IN),(LJIN,JN) +******* PERFORMS LOGICAL OPERATIONS ON INTEGER VARIABLES +******* SO COULD BE FLAGGED AS ILLEGAL OR GIVE INCORRECT RESULTS +******* ON MANY MACHINES CAN BE REPLACED BY LOCAL INTRINSIC IOR +C IN=IIN +C JN=JIN +C LIIN=LIIN.OR.LJIN +C Sun +C IORRS=OR(IIN,JIN) +C Alliant + IORRS=IOR(IIN,JIN) + RETURN + END + SUBROUTINE WRITEB(CHARS,NCHAR,IDEVGR) +C AUTHOR: RODGER STADEN + CHARACTER CHARS*(*) + SAVE NUL + DATA NUL/0/ +C NOTE NULS STOP CARRIAGE RETURN, LINE FEED +C Sun +C WRITE(IDEVGR,1000)CHARS(1:NCHAR) +C1000 FORMAT(A,$) +C Alliant + WRITE(IDEVGR,1000)NUL,CHARS(1:NCHAR) +1000 FORMAT(A1,A) + RETURN + END + SUBROUTINE SABIT(ARRAY,MAXAR,POSN) + INTEGER ARRAY(0:MAXAR),POSN,ELEMNT,BITNUM +C +C set bit corresponding to posn in array (bits 0-31 per word) +C + I = POSN - 1 + ELEMNT = I / 32 + BITNUM = MOD(I,32) +C WRITE(*,*)'ELEMENT, BIT',ELEMNT,BITNUM + CALL SBITRS(ARRAY(ELEMNT),BITNUM) + END + SUBROUTINE AAWORD(ARRAY1,ARRAY2,MAXAR) + INTEGER ARRAY1(0:MAXAR),ARRAY2(0:MAXAR) + INTEGER IANDRS + EXTERNAL IANDRS +C +C and array1 and array2 and put result in array1 +C + DO 10 I=0,MAXAR + ARRAY1(I) = IANDRS(ARRAY1(I),ARRAY2(I)) + 10 CONTINUE + END + SUBROUTINE OAWORD(ARRAY1,ARRAY2,MAXAR) + INTEGER ARRAY1(0:MAXAR),ARRAY2(0:MAXAR) + INTEGER IORRS + EXTERNAL IORRS +C +C OR array1 and array2 and put result in array1 +C + DO 10 I=0,MAXAR + ARRAY1(I) = IORRS(ARRAY1(I),ARRAY2(I)) + 10 CONTINUE + END + LOGICAL FUNCTION TABIT(ARRAY,MAXAR,POSN) + INTEGER ARRAY(0:MAXAR),POSN,ELEMNT,BIT + LOGICAL TBITRS + EXTERNAL TBITRS +C +C test bit corresponding to posn in array (bits 0-31 per word) +C + I = POSN - 1 + ELEMNT = I / 32 + BIT = MOD(I,32) +C WRITE(*,*)'TEST ELEMENT, BIT',ELEMNT,BIT + TABIT = TBITRS(ARRAY(ELEMNT),BIT) + END + SUBROUTINE CABIT(ARRAY,MAXAR) + INTEGER ARRAY(0:MAXAR),POSN +C +C clear all bits in array (1 at a time!!!!!!!!) +C + DO 10 POSN=0,MAXAR + DO 5 J = 0,31 + CALL CBITRS(ARRAY(POSN),J) + 5 CONTINUE + 10 CONTINUE + END + SUBROUTINE TBITA(ARRAY,MAXAR,IDEV) + INTEGER ARRAY(0:MAXAR) + LOGICAL TBITRS + EXTERNAL TBITRS +C +C test all bits in array to see if they are set +C + DO 10 I=0,MAXAR + DO 5 J=0,31 + IF(TBITRS(ARRAY(I),J)) THEN + K = I*32 + 1 + MOD(J,32) + WRITE(IDEV,*)' BIT SET FOR POSN',K + END IF + 5 CONTINUE + 10 CONTINUE + END +C routines to handle bits (machine specific) for use when +C searching through keyword indexes +C +C positions for marking are numbered from 1 onwards +C but array elements and bits are numbered from 0 onwards +C + SUBROUTINE SBITRS(WORD,BITNUM) +C +C set bit bitnum in word +C + INTEGER WORD,BITNUM +C alliant + INTEGER IBSET + INTRINSIC IBSET + WORD = IBSET(WORD,BITNUM) +C sun +C CALL BIS(BITNUM,WORD) + END + SUBROUTINE CBITRS(WORD,BITNUM) +C +C clear bit bitnum in word +C + INTEGER WORD,BITNUM +C alliant + INTEGER IBCLR + INTRINSIC IBCLR + WORD = IBCLR(WORD,BITNUM) +C sun +C CALL BIC(BITNUM,WORD) + END + LOGICAL FUNCTION TBITRS(WORD,BITNUM) +C +C test bit bitnum in word +C + INTEGER WORD,BITNUM +C alliant + LOGICAL BTEST + INTRINSIC BTEST + TBITRS = BTEST(WORD,BITNUM) +C sun +C LOGICAL BIT +C EXTERNAL BIT +C TBITRS = BIT(BITNUM,WORD) + END + INTEGER FUNCTION GETRS(IDEV,STRING,NSTRNG,IBYTE) + CHARACTER STRING*(*) + IREC = IBYTE + DO 10 I=1,NSTRNG + READ(IDEV,REC=IREC,ERR=20)STRING(I:I) + IREC = IREC + 1 + 10 CONTINUE + GETRS = 0 + RETURN + 20 CONTINUE + GETRS = -1 + END + INTEGER FUNCTION GETRSL(IDEV,STRING,NSTRNG) + CHARACTER STRING*(*) + CHARACTER CR + PARAMETER (CR=CHAR(13)) + READ(IDEV,1000,ERR=100,END=200)STRING(1:NSTRNG) + 1000 FORMAT(A) + DO 10 I=1,NSTRNG + IF(STRING(I:I).EQ.CR) THEN + GETRSL = I-1 + RETURN + END IF + 10 CONTINUE + GETRSL = NSTRNG + RETURN + 100 CONTINUE + WRITE(*,*)'ERROR READING IN GETRSL' + GETRSL = -2 + RETURN + 200 CONTINUE + GETRSL = -1 + WRITE(*,*)'END IN GETRSL' + END + INTEGER FUNCTION SEEKRS(IDEV,IREC) + INTEGER FSEEK +C OLD VERSION THIS LINE ONLY SEEKRS = FSEEK(IDEV,IREC,0) +C following 4 lines apparently fix the bug! + COMMON /ALBUGS/I,J,K + K = 0 + J = IREC + I = FSEEK(IDEV,J,K) + SEEKRS = I + END diff --git a/src/staden/seeme-dec.f b/src/staden/seeme-dec.f new file mode 100644 index 0000000..0196c51 --- /dev/null +++ b/src/staden/seeme-dec.f @@ -0,0 +1,674 @@ +C SEEME: MACHINE SPECIFIC ROUTINES for dec !!!!!!!!! +C DEC ULTRIX cahnges: iwordl = 1, seqlibs open 'stream' +C 14-8-91 Added check in openrs for empty file names +C 27-8-91 Started files xspec.f and nxspec.f and split out the nonx +C and X specific bits from here. +C 27-8-91 Added FLUSHL to flush unit idev +C 15-10-91 Moved IANDRS, IORRS and WRITEB from pl4010.f +C 29-10-91 Replaced lnblnk ( a sun routine) by notrl +C 15-11-91 Added routines to call inquire to see if a file exists +C and another to delete a file +C 25-2-92 added more bit handling routines for use by sequence library +C searching routines +C 20-5-92 Added readonly where required for dec! +C moved getrs, getrsl and seekrs from seqlibsubs.f to here +C AUTHOR: RODGER STADEN +C 25-JAN-1990 INILU: SAVE DUP,PUP,DLOW,PLOW +C UNITNO +C OPENRS +C THE FOLLOWING ARE PROBABLY COMPLETELY PORTABLE +C BUT ARE INCLUDED ANY WAY +C INITLU +C IFROMP +C CTONUM +C DTONUM +C IUBM +C IUBM1 +C IUBM2 +C bsw4 +C bsw2 + SUBROUTINE UNITNO(KBIN,KBOUT,DEVNOS,NDEVS) +C AUTHOR: RODGER STADEN + INTEGER DEVNOS(NDEVS) +C SETS CONSOLE I/O UNITS TO 5,6. OTHERS 10 ONWARDS + KBIN=5 + KBOUT=6 + ISTART=10 + DO 1 I=1,NDEVS + DEVNOS(I)=ISTART+I-1 +1 CONTINUE + CALL INITRS() + END + SUBROUTINE OPENRS(IDEV,FN,IOK,LRECL,JOB) +C AUTHOR: RODGER STADEN + CHARACTER FN*(*) + CHARACTER*1024 FILNAM,FNDFIL + EXTERNAL FNDFIL,NOTRL,INQF +C 14-8-91 Added check for blank file names +C 14-11-91 Added routines to check for file existence +C ROUTINE TO OPEN FILES +C JOB=1 SEQUENTIAL FORMATTED NEW, DEFAULT RECORD LENGTH +C =2 SEQUENTIAL FORMATTED OLD, DEFAULT RECORD LENGTH +C =3 DIRECT ACCESS, UNFORMATTED NEW +C =4 DIRECT ACCESS, UNFORMATTED OLD +C =5 DIRECT ACCESS, UNFORMATTED OLD +C =6 UNFORMATTED NEW +C =7 UNFORMATTED OLD +C =8 STATUS='UNKNOWN' +C =9 TERMINAL FOR GRAPHICS +C =10 TERMINAL FOR VT100 +C =11 direct access, single byte record length, old +C RECORD LENGTHS SENT IN WORDS +C READONLY IS VAX SPECIFIC********************* +C NOTE THAT ONLY FOR DIRECT ACCESS FILES ARE RECORD +C LENGTHS SPECIFIED. ON THE VAX UNFORMATTED DIRECT ACCESS +C RECORDS ARE DEFINED IN WORDS; ON OTHER MACHINES E.G. SOME UNIX +C MACHINES THEY ARE SPECIFIED IN BYTES. THE VARIABLE IWORDL IS +C USED TO MULTIPLY RECORD LENGTHS SPECIFIED IN WORDS TO GIVE +C THE CORRECT VALUE FOR THE MACHINE. ON A VAX IWORDL=1; ON +C THE UNIX MACHINE IWORDL=4 + PARAMETER (IWORDL=1) + IF(NOTRL(FN,LEN(FN),' ').EQ.0) GO TO 100 + IF (JOB.LT.9 .OR. JOB.GT.10) THEN + FILNAM = FNDFIL(FN) + INQJ = INQF(FILNAM) + END IF + IF(JOB.EQ.1) THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + ACCESS='SEQUENTIAL',ERR=100) + ELSE IF(JOB.EQ.2)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + READONLY, + + ACCESS='SEQUENTIAL',ERR=100) + ELSE IF(JOB.EQ.3)THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.4)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.5)THEN + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + READONLY, + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.6)THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + FORM='UNFORMATTED',ERR=100) + ELSE IF(JOB.EQ.7)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + READONLY, + + FORM='UNFORMATTED',ERR=100) + ELSE IF(JOB.EQ.8)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.9)THEN +C DEVICE FOR GRAPHICS OUTPUT IS 'TT' IF USING A +C VT640 ON A VAX. IF USING A SEPARATE TERMINAL FOR +C GRAPHICS OUTPUT THE TERMINAL PROTECTION MUST BE +C SET W:RW AND THE DEVICE WILL HAVE A DIFFERENT NAME +C FOR EXAMPLE 'GRAPHICS' WHERE THE LOGICAL NAME 'GRAPHICS' +C IS ASSIGNED TO THE APPROPRIATE DEVICE. A VT240 SHOULD +C BE COMPATIBLE WITH THE TEKTRONIX PLOT COMMANDS. + OPEN(UNIT=IDEV,FILE='/dev/tty',STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.10)THEN +C DEVICE FOR VT100 COMPATIBLE TERMINAL WHEN USED +C FOR GELIN WHICH REQUIRES COMPLETE CONTROL OF +C CURSOR POSITIONING. ON A VAX IT IS CALLED 'TT' + OPEN(UNIT=IDEV,FILE='/dev/tty',STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.11)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + JRECL=1 + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + READONLY, + + RECORDTYPE='STREAM',ERR=100) + END IF + IOK=0 + RETURN +100 CONTINUE + IOK=1 + RETURN + END + CHARACTER*1024 FUNCTION FNDFIL(NAME) + CHARACTER NAME*(*),TMPNAM*1024,PREFIX*1024 + EXTERNAL NOTRL +C LOOKS IN ENVIRONMENT FOR FILE PATHS +C ? CASES DEALT WITH: +C 1) IF NAME STARTS WITH / IT IS ABSOLUTE AND SO UNCHANGED +C 2) IF NAME STARTS WITH ~/ REPLACE WITH HOME DIRECTORY NAME +C 3) IF NAME INCLUDES ANY OTHER / LOOK IN ENVIRONMENT FOR +C NAME UPTO /, AND ADD PATH TO REST IF FOUND +C IF NOT FOUND ADD PATH TO CURRENT WORKING DIRECTORY +C 4) ELSE LOOK IN ENVIRONMENT FOR WHOLE NAME +C IF NOT FOUND ADD PATH TO CURRENT DIRECTORY +C IF FOUND MAKE IT FILE NAME +C + IF(NAME(1:1).EQ.'/') THEN + FNDFIL = NAME + ELSE IF (NAME(1:2).EQ.'~/') THEN + CALL GETENV('HOME',TMPNAM) + FNDFIL = TMPNAM(:NOTRL(TMPNAM,LEN(TMPNAM),' ')) // + + NAME(2:NOTRL(NAME,LEN(NAME),' ')) + ELSE IF (INDEX(NAME,'/').NE.0) THEN + TMPNAM = NAME(1:INDEX(NAME,'/')-1) + CALL GETENV(TMPNAM,PREFIX) + I = NOTRL(PREFIX,LEN(PREFIX),' ') + IF(I.EQ.0) THEN + CALL GETCWD(PREFIX) + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + '/' // NAME(:NOTRL(NAME,LEN(NAME),' ')) + ELSE + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + NAME(INDEX(NAME,'/'):) + END IF + ELSE + CALL GETENV(NAME,TMPNAM) + I = NOTRL(TMPNAM,LEN(TMPNAM),' ') + IF (I.EQ.0) THEN + CALL GETCWD(PREFIX) + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + '/' // NAME(:NOTRL(NAME,LEN(NAME),' ')) + ELSE + FNDFIL = TMPNAM + END IF + END IF + END + INTEGER FUNCTION INQF(NAME) + CHARACTER NAME*(*) + LOGICAL EX + INQUIRE(FILE=NAME,EXIST=EX) +C +C if the file exists return 1, else 0 +C + IF (EX) THEN + INQF = 1 + ELSE + INQF = 0 + END IF + END + INTEGER FUNCTION DELF(FN,IDEV,JRECL,JOB) + CHARACTER FN*(*) + CHARACTER FILNAM*1024,FNDFIL*1024 + EXTERNAL FNDFIL +C +C routine to delete files in the most horrible way i could find +C deal with job 2: old sequential formatted +C deal with job 4: old direct unformatted +C deal with job 7: old sequential unformatted +C if the file exists and is not one of these error =-1 +C if the file exists and cannot be deleted error =-2 +C if the file cannot be opened error =-3 +C +C on the sun this technique will delete any file if the directory +C priviliges are set. +C I dont know if i need to worry about the file types: could +C i just open them all the same way? Although its a mess leave it! + FILNAM = FNDFIL(FN) + IF(JOB.EQ.2) THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='SEQUENTIAL',ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE IF(JOB.EQ.4)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE IF(JOB.EQ.7)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + FORM='UNFORMATTED',ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE + DELF = -1 + RETURN + END IF + RETURN + 100 CONTINUE + DELF = -3 + RETURN + 200 CONTINUE + DELF = -2 + END +C ROUTINES TO CONTROL CHARACTER LOOKUP +C FOR BOTH DNA AND PROTEIN SEQUENCES +C THE INITIALISING ROUTINES ARE SENT THE CHARACTERSET SIZE IDM +C WHICH DETERMINES WHICH CHARACTERSET IS USED + SUBROUTINE INITLU(IDM) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255),POINT2(0:255) + CHARACTER DUP*16,DLOW*16,PUP*26,PLOW*26 + COMMON /IASCI1/POINT1 + COMMON /IASCI2/POINT2 + SAVE /IASCI1/ + SAVE /IASCI2/ + SAVE DUP,PUP,DLOW,PLOW + DATA DUP/'TCAG-RYWSMKHBVDN'/ + DATA PUP/'CSTPAGNDEQBZHRKMILVFYW-X? '/ + DATA DLOW/'tcag-rywsmkhbvdn'/ + DATA PLOW/'cstpagndeqbzhrkmilvfyw-x? '/ +C ICHAR RETURNS THE COLLATING SEQUENCE NUMBER +C I WANT 1-5 FOR ACGT OR 1-26 FOR AMINO ACIDS BY USING ICHAR. +C THE ACTUAL VALUE RETURNED BY ICHAR IS NOT PORTABLE +C SO I NEED TO INITIALIZE POINTR SO THAT THE CORRECT +C ELEMENTS CONTAIN VALUES 1 - 5, OR 1 - 26 +C WORKS ON UPPER AND LOWER CASE - REMOVE DLOW,PLOW AND LOOPS 41 AND 51 +C IF LOWERCASE NOT ALLOWED +C + IF(IDM.EQ.5)THEN + DO 30 I = 0,255 + POINT1(I) = IDM + POINT2(I) = 17 +30 CONTINUE + DO 35 I = 1,5 + J = ICHAR(DUP(I:I)) + POINT1(J) = I +35 CONTINUE + DO 36 I = 1,5 + J = ICHAR(DLOW(I:I)) + POINT1(J) = I +36 CONTINUE + DO 40 I = 1,16 + J = ICHAR(DUP(I:I)) + POINT2(J) = I +40 CONTINUE +C DEAL WITH U + J = ICHAR('U') + POINT1(J) = 1 + POINT2(J) = 1 + DO 41 I = 1,16 + J = ICHAR(DLOW(I:I)) + POINT2(J) = I +41 CONTINUE +C DEAL WITH U + J = ICHAR('u') + POINT1(J) = 1 + POINT2(J) = 1 + ELSE IF(IDM.EQ.26)THEN + DO 45 I = 0,255 + POINT1(I) = IDM +45 CONTINUE +C + DO 50 I = 1,26 + J = ICHAR(PUP(I:I)) + POINT1(J) = I +50 CONTINUE + DO 51 I = 1,26 + J = ICHAR(PLOW(I:I)) + POINT1(J) = I +51 CONTINUE + DO 60 I = 0,255 + POINT2(I) = POINT1(I) +60 CONTINUE + ELSE + WRITE(*,*)'ERROR INITIALISING CHARACTER LOOKUP POINTERS' + END IF + END + INTEGER FUNCTION IFROMP(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255) + CHARACTER CHAR + COMMON /IASCI1/POINT1 + SAVE /IASCI1/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + IFROMP = POINT1(ICOL) + END + INTEGER FUNCTION CTONUM(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255) + CHARACTER CHAR + COMMON /IASCI1/POINT1 + SAVE /IASCI1/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + CTONUM = POINT1(ICOL) + END + INTEGER FUNCTION DTONUM(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT2(0:255) + CHARACTER CHAR + COMMON /IASCI2/POINT2 + SAVE /IASCI2/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + DTONUM = POINT2(ICOL) + END + INTEGER FUNCTION IUBM(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A DEFINITE MATCH, 2 FOR POSSIBLE, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,2,0,2,2,0,0,2,2,2,0,2,2,0, + +0,1,0,0,2,0,2,0,2,2,0,2,2,2,0,2,0, + +0,0,1,0,2,2,0,2,0,2,0,2,0,2,2,2,0, + +0,0,0,1,2,2,0,0,2,0,2,0,2,2,2,2,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,2,1,0,2,2,2,2,2,2,2,2,2,0, + +1,1,0,0,2,0,1,2,2,2,2,2,2,2,2,2,0, + +1,0,1,0,2,2,2,1,0,2,2,2,2,2,2,2,0, + +0,1,0,1,2,2,2,0,1,2,2,2,2,2,2,2,0, + +0,1,1,0,2,2,2,2,2,1,0,2,2,2,2,2,0, + +1,0,0,1,2,2,2,2,2,2,1,2,2,2,2,2,0, + +1,1,1,0,2,2,2,1,2,1,2,1,2,2,2,2,0, + +1,1,0,1,2,2,1,2,1,2,1,2,1,2,2,2,0, + +0,1,1,1,2,1,2,2,1,1,2,2,2,1,2,2,0, + +1,0,1,1,2,1,2,1,2,2,1,2,2,2,1,2,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END + INTEGER FUNCTION IUBM2(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A POSSIBLE MATCH, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,1,0,1,1,0,0,1,1,1,0,1,1,0, + +0,1,0,0,1,0,1,0,1,1,0,1,1,1,0,1,0, + +0,0,1,0,1,1,0,1,0,1,0,1,0,1,1,1,0, + +0,0,0,1,1,1,0,0,1,0,1,0,1,1,1,1,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,1,1,0,1,1,1,1,1,1,1,1,1,0, + +1,1,0,0,1,0,1,1,1,1,1,1,1,1,1,1,0, + +1,0,1,0,1,1,1,1,0,1,1,1,1,1,1,1,0, + +0,1,0,1,1,1,1,0,1,1,1,1,1,1,1,1,0, + +0,1,1,0,1,1,1,1,1,1,0,1,1,1,1,1,0, + +1,0,0,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,1,0,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,0,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +0,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,0,1,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM2 = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END + INTEGER FUNCTION IUBM1(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A DEFINITE MATCH, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,0,1,0,0,0,0,0,0,0,0,0,0,0, + +1,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0,0, + +1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0, + +0,1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0, + +0,1,1,0,0,0,0,0,0,1,0,0,0,0,0,0,0, + +1,0,0,1,0,0,0,0,0,0,1,0,0,0,0,0,0, + +1,1,1,0,0,0,0,1,0,1,0,1,0,0,0,0,0, + +1,1,0,1,0,0,1,0,1,0,1,0,1,0,0,0,0, + +0,1,1,1,0,1,0,0,1,1,0,0,0,1,0,0,0, + +1,0,1,1,0,1,0,1,0,0,1,0,0,0,1,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM1 = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END +C +C + SUBROUTINE FLUSHL(IDEV) + CALL FLUSH(IDEV) +C This implementation uses the Sun Fortran FLUSH statement. + END + SUBROUTINE FLUSHO +C The (single) unit number is currently hard-wired, but must +C be the same as KBOUT in UNITNO. + CALL FLUSHL(6) + END +C + SUBROUTINE CEDIT(FILNAM) + CHARACTER COMAND*256,EDITOR*256,FILNAM*(*) + INTEGER SYSTEM,I + EXTERNAL NOTRL + CALL GETENV('SEQEDT',EDITOR) + I=NOTRL(EDITOR,LEN(EDITOR),' ') + IF (I.EQ.0) THEN + EDITOR='vi' + I=2 + ENDIF + COMAND = EDITOR(1:I)//' '//FILNAM + ISTAT = SYSTEM(COMAND) + END + SUBROUTINE BSW2(IN,OUT) +C byte order on cd is least significant first +C on some machines this routine should be changed to do nothing + CHARACTER*2 IN,OUT +C Sun +C OUT(1:1) = IN(2:2) +C OUT(2:2) = IN(1:2) +C Alliant + OUT = IN + END + SUBROUTINE BSW4(IN,OUT) +C byte order on cd is least significant first +C on some machines this routine should be changed to do nothing + CHARACTER IN*4,OUT*4 +C Sun +C DO 10 I = 1,2 +C OUT(I:I) = IN(5-I:5-I) +C OUT(5-I:5-I) = IN(I:I) +C 10 CONTINUE +C Alliant + OUT = IN + END + INTEGER FUNCTION IANDRS(IIN,JIN) +C AUTHOR: RODGER STADEN +C LOGICAL LIIN,LJIN +C INTEGER IN,JN +C EQUIVALENCE (LIIN,IN),(LJIN,JN) +******* PERFORMS LOGICAL OPERATIONS ON INTEGER VARIABLES +******* SO COULD BE FLAGGED AS ILLEGAL OR GIVE INCORRECT RESULTS +******* ON MANY MACHINES CAN BE REPLACED BY LOCAL INTRINSIC IAND +C IN=IIN +C JN=JIN +C LIIN=LIIN.AND.LJIN +C dec + IANDRS=IAND(IIN,JIN) +C Alliant +C IANDRS=IAND(IIN,JIN) + END + INTEGER FUNCTION IORRS(IIN,JIN) +C AUTHOR: RODGER STADEN +C LOGICAL LIIN,LJIN +C INTEGER IN,JN +C EQUIVALENCE (LIIN,IN),(LJIN,JN) +******* PERFORMS LOGICAL OPERATIONS ON INTEGER VARIABLES +******* SO COULD BE FLAGGED AS ILLEGAL OR GIVE INCORRECT RESULTS +******* ON MANY MACHINES CAN BE REPLACED BY LOCAL INTRINSIC IOR +C IN=IIN +C JN=JIN +C LIIN=LIIN.OR.LJIN +C dec + IORRS=IOR(IIN,JIN) +C Alliant +C IORRS=IOR(IIN,JIN) + END + SUBROUTINE WRITEB(CHARS,NCHAR,IDEVGR) +C AUTHOR: RODGER STADEN + CHARACTER CHARS*(*) + SAVE NUL + DATA NUL/0/ +C NOTE NULS STOP CARRIAGE RETURN, LINE FEED +C Sun + WRITE(IDEVGR,1000)CHARS(1:NCHAR) +1000 FORMAT(A,$) +C Alliant +C WRITE(IDEVGR,1000)NUL,CHARS(1:NCHAR) +C1000 FORMAT(A1,A) + END + SUBROUTINE SABIT(ARRAY,MAXAR,POSN) + INTEGER ARRAY(0:MAXAR),POSN,ELEMNT,BITNUM +C +C set bit corresponding to posn in array (bits 0-31 per word) +C + I = POSN - 1 + ELEMNT = I / 32 + BITNUM = MOD(I,32) +C WRITE(*,*)'ELEMENT, BIT',ELEMNT,BITNUM + CALL SBITRS(ARRAY(ELEMNT),BITNUM) + END + SUBROUTINE AAWORD(ARRAY1,ARRAY2,MAXAR) + INTEGER ARRAY1(0:MAXAR),ARRAY2(0:MAXAR) + INTEGER IANDRS + EXTERNAL IANDRS +C +C and array1 and array2 and put result in array1 +C + DO 10 I=0,MAXAR + ARRAY1(I) = IANDRS(ARRAY1(I),ARRAY2(I)) + 10 CONTINUE + END + SUBROUTINE OAWORD(ARRAY1,ARRAY2,MAXAR) + INTEGER ARRAY1(0:MAXAR),ARRAY2(0:MAXAR) + INTEGER IORRS + EXTERNAL IORRS +C +C OR array1 and array2 and put result in array1 +C + DO 10 I=0,MAXAR + ARRAY1(I) = IORRS(ARRAY1(I),ARRAY2(I)) + 10 CONTINUE + END + LOGICAL FUNCTION TABIT(ARRAY,MAXAR,POSN) + INTEGER ARRAY(0:MAXAR),POSN,ELEMNT,BIT + LOGICAL TBITRS + EXTERNAL TBITRS +C +C test bit corresponding to posn in array (bits 0-31 per word) +C + I = POSN - 1 + ELEMNT = I / 32 + BIT = MOD(I,32) +C WRITE(*,*)'TEST ELEMENT, BIT',ELEMNT,BIT + TABIT = .FALSE. + IF (ARRAY(ELEMNT).NE.0) THEN + TABIT = TBITRS(ARRAY(ELEMNT),BIT) + END IF + END + SUBROUTINE CABIT(ARRAY,MAXAR) + INTEGER ARRAY(0:MAXAR),POSN +C +C clear all bits in array (1 word at a time) +C + DO 10 POSN=0,MAXAR + ARRAY(POSN) = 0 + 10 CONTINUE + END + SUBROUTINE SBITRS(WORD,BITNUM) + INTEGER MASKS(0:31),WORD,BITNUM + EXTERNAL IORRS + SAVE MASKS + DATA MASKS/1,2,4,8,16,32,64,128,256,512,1024,2048,4096, + +8192,16384,32768,65536,131072,262144,524288,1048576, + +2097152,4194304,8388608,16777216,33554432,67108864, + +134217728,268435456,536870912,1073741824,-2147483648/ + WORD = IORRS(WORD,MASKS(BITNUM)) + END + LOGICAL FUNCTION TBITRS(WORD,BITNUM) + INTEGER MASKS(0:31),WORD,BITNUM + EXTERNAL IANDRS + SAVE MASKS + DATA MASKS/1,2,4,8,16,32,64,128,256,512,1024,2048,4096, + +8192,16384,32768,65536,131072,262144,524288,1048576, + +2097152,4194304,8388608,16777216,33554432,67108864, + +134217728,268435456,536870912,1073741824,-2147483648/ + I = MASKS(BITNUM) + TBITRS = (IANDRS(WORD,I).EQ.I) + END + INTEGER FUNCTION GETRS(IDEV,STRING,NSTRNG,IBYTE) + CHARACTER STRING*(*) + INTEGER SEEKRS + EXTERNAL SEEKRS + GETRS = SEEKRS(IDEV,IBYTE-1) + IF(GETRS.NE.0) RETURN + READ(IDEV,1000,ERR=20,END=20)STRING(1:NSTRNG) + 1000 FORMAT(A) + GETRS = 0 + RETURN + 20 CONTINUE + GETRS = -1 + END + INTEGER FUNCTION GETRSL(IDEV,STRING,NSTRNG) + CHARACTER STRING*(*) + CHARACTER CR + PARAMETER (CR=CHAR(13)) + READ(IDEV,1000,ERR=100,END=200)STRING(1:NSTRNG) + 1000 FORMAT(A) + DO 10 I=1,NSTRNG + IF(STRING(I:I).EQ.CR) THEN + GETRSL = I-1 + RETURN + END IF + 10 CONTINUE + GETRSL = NSTRNG + RETURN + 100 CONTINUE + WRITE(*,*)'ERROR READING IN GETRSL' + GETRSL = -2 + RETURN + 200 CONTINUE + GETRSL = -1 + WRITE(*,*)'END IN GETRSL' + END + INTEGER FUNCTION SEEKRS(IDEV,IREC) + INTEGER FSEEK + SEEKRS = FSEEK(IDEV,IREC,0) + END diff --git a/src/staden/seeme-sg.f b/src/staden/seeme-sg.f new file mode 100644 index 0000000..28bed53 --- /dev/null +++ b/src/staden/seeme-sg.f @@ -0,0 +1,680 @@ +C SEEME: MACHINE SPECIFIC ROUTINES for silicon graphics +C 14-8-91 Added check in openrs for empty file names +C 27-8-91 Started files xspec.f and nxspec.f and split out the nonx +C and X specific bits from here. +C 27-8-91 Added FLUSHL to flush unit idev +C 15-10-91 Moved IANDRS, IORRS and WRITEB from pl4010.f +C 29-10-91 Replaced lnblnk ( a sun routine) by notrl +C 15-11-91 Added routines to call inquire to see if a file exists +C and another to delete a file +C 25-2-92 added more bit handling routines for use by sequence library +C searching routines +C 20-5-92 Added readonly where required for dec! +C moved getrs, getrsl and seekrs from seqlibsubs.f to here +C AUTHOR: RODGER STADEN +C 25-JAN-1990 INILU: SAVE DUP,PUP,DLOW,PLOW +C UNITNO +C OPENRS +C THE FOLLOWING ARE PROBABLY COMPLETELY PORTABLE +C BUT ARE INCLUDED ANY WAY +C INITLU +C IFROMP +C CTONUM +C DTONUM +C IUBM +C IUBM1 +C IUBM2 +C bsw4 +C bsw2 + SUBROUTINE UNITNO(KBIN,KBOUT,DEVNOS,NDEVS) +C AUTHOR: RODGER STADEN + INTEGER DEVNOS(NDEVS) +C SETS CONSOLE I/O UNITS TO 5,6. OTHERS 10 ONWARDS + KBIN=5 + KBOUT=6 + ISTART=10 + DO 1 I=1,NDEVS + DEVNOS(I)=ISTART+I-1 +1 CONTINUE + CALL INITRS() + END + SUBROUTINE OPENRS(IDEV,FN,IOK,LRECL,JOB) +C AUTHOR: RODGER STADEN + CHARACTER FN*(*) + CHARACTER*1024 FILNAM,FNDFIL + EXTERNAL FNDFIL,NOTRL,INQF +C 14-8-91 Added check for blank file names +C 14-11-91 Added routines to check for file existence +C ROUTINE TO OPEN FILES +C JOB=1 SEQUENTIAL FORMATTED NEW, DEFAULT RECORD LENGTH +C =2 SEQUENTIAL FORMATTED OLD, DEFAULT RECORD LENGTH +C =3 DIRECT ACCESS, UNFORMATTED NEW +C =4 DIRECT ACCESS, UNFORMATTED OLD +C =5 DIRECT ACCESS, UNFORMATTED OLD +C =6 UNFORMATTED NEW +C =7 UNFORMATTED OLD +C =8 STATUS='UNKNOWN' +C =9 TERMINAL FOR GRAPHICS +C =10 TERMINAL FOR VT100 +C =11 direct access, single byte record length, old +C RECORD LENGTHS SENT IN WORDS +C READONLY IS VAX SPECIFIC********************* +C NOTE THAT ONLY FOR DIRECT ACCESS FILES ARE RECORD +C LENGTHS SPECIFIED. ON THE VAX UNFORMATTED DIRECT ACCESS +C RECORDS ARE DEFINED IN WORDS; ON OTHER MACHINES E.G. SOME UNIX +C MACHINES THEY ARE SPECIFIED IN BYTES. THE VARIABLE IWORDL IS +C USED TO MULTIPLY RECORD LENGTHS SPECIFIED IN WORDS TO GIVE +C THE CORRECT VALUE FOR THE MACHINE. ON A VAX IWORDL=1; ON +C THE UNIX MACHINE IWORDL=4 + PARAMETER (IWORDL=4) + IF(NOTRL(FN,LEN(FN),' ').EQ.0) GO TO 100 + IF (JOB.LT.9 .OR. JOB.GT.10) THEN + FILNAM = FNDFIL(FN) + INQJ = INQF(FILNAM) + END IF + IF(JOB.EQ.1) THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + ACCESS='SEQUENTIAL',ERR=100) + ELSE IF(JOB.EQ.2)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + READONLY, + + ACCESS='SEQUENTIAL',ERR=100) + ELSE IF(JOB.EQ.3)THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.4)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.5)THEN + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + READONLY, + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.6)THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + FORM='UNFORMATTED',ERR=100) + ELSE IF(JOB.EQ.7)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + READONLY, + + FORM='UNFORMATTED',ERR=100) + ELSE IF(JOB.EQ.8)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.9)THEN +C DEVICE FOR GRAPHICS OUTPUT IS 'TT' IF USING A +C VT640 ON A VAX. IF USING A SEPARATE TERMINAL FOR +C GRAPHICS OUTPUT THE TERMINAL PROTECTION MUST BE +C SET W:RW AND THE DEVICE WILL HAVE A DIFFERENT NAME +C FOR EXAMPLE 'GRAPHICS' WHERE THE LOGICAL NAME 'GRAPHICS' +C IS ASSIGNED TO THE APPROPRIATE DEVICE. A VT240 SHOULD +C BE COMPATIBLE WITH THE TEKTRONIX PLOT COMMANDS. + OPEN(UNIT=IDEV,FILE='/dev/tty',STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.10)THEN +C DEVICE FOR VT100 COMPATIBLE TERMINAL WHEN USED +C FOR GELIN WHICH REQUIRES COMPLETE CONTROL OF +C CURSOR POSITIONING. ON A VAX IT IS CALLED 'TT' + OPEN(UNIT=IDEV,FILE='/dev/tty',STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.11)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + JRECL=1 + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + READONLY,ACCESS='DIRECT',RECL=1,ERR=100) + END IF + IOK=0 + RETURN +100 CONTINUE + IOK=1 + RETURN + END + CHARACTER*1024 FUNCTION FNDFIL(NAME) + CHARACTER NAME*(*),TMPNAM*1024,PREFIX*1024 + EXTERNAL NOTRL +C LOOKS IN ENVIRONMENT FOR FILE PATHS +C ? CASES DEALT WITH: +C 1) IF NAME STARTS WITH / IT IS ABSOLUTE AND SO UNCHANGED +C 2) IF NAME STARTS WITH ~/ REPLACE WITH HOME DIRECTORY NAME +C 3) IF NAME INCLUDES ANY OTHER / LOOK IN ENVIRONMENT FOR +C NAME UPTO /, AND ADD PATH TO REST IF FOUND +C IF NOT FOUND ADD PATH TO CURRENT WORKING DIRECTORY +C 4) ELSE LOOK IN ENVIRONMENT FOR WHOLE NAME +C IF NOT FOUND ADD PATH TO CURRENT DIRECTORY +C IF FOUND MAKE IT FILE NAME +C + IF(NAME(1:1).EQ.'/') THEN + FNDFIL = NAME + ELSE IF (NAME(1:2).EQ.'~/') THEN + CALL GETENV('HOME',TMPNAM) + FNDFIL = TMPNAM(:NOTRL(TMPNAM,LEN(TMPNAM),' ')) // + + NAME(2:NOTRL(NAME,LEN(NAME),' ')) + ELSE IF (INDEX(NAME,'/').NE.0) THEN + TMPNAM = NAME(1:INDEX(NAME,'/')-1) + CALL GETENV(TMPNAM,PREFIX) + I = NOTRL(PREFIX,LEN(PREFIX),' ') + IF(I.EQ.0) THEN + CALL GETCWD(PREFIX) + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + '/' // NAME(:NOTRL(NAME,LEN(NAME),' ')) + ELSE + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + NAME(INDEX(NAME,'/'):) + END IF + ELSE + CALL GETENV(NAME,TMPNAM) + I = NOTRL(TMPNAM,LEN(TMPNAM),' ') + IF (I.EQ.0) THEN + CALL GETCWD(PREFIX) + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + '/' // NAME(:NOTRL(NAME,LEN(NAME),' ')) + ELSE + FNDFIL = TMPNAM + END IF + END IF + END + INTEGER FUNCTION INQF(NAME) + CHARACTER NAME*(*) + LOGICAL EX + INQUIRE(FILE=NAME,EXIST=EX) +C +C if the file exists return 1, else 0 +C + IF (EX) THEN + INQF = 1 + ELSE + INQF = 0 + END IF + END + INTEGER FUNCTION DELF(FN,IDEV,JRECL,JOB) + CHARACTER FN*(*) + CHARACTER FILNAM*1024,FNDFIL*1024 + EXTERNAL FNDFIL +C +C routine to delete files in the most horrible way i could find +C deal with job 2: old sequential formatted +C deal with job 4: old direct unformatted +C deal with job 7: old sequential unformatted +C if the file exists and is not one of these error =-1 +C if the file exists and cannot be deleted error =-2 +C if the file cannot be opened error =-3 +C +C on the sun this technique will delete any file if the directory +C priviliges are set. +C I dont know if i need to worry about the file types: could +C i just open them all the same way? Although its a mess leave it! + FILNAM = FNDFIL(FN) + IF(JOB.EQ.2) THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='SEQUENTIAL',ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE IF(JOB.EQ.4)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE IF(JOB.EQ.7)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + FORM='UNFORMATTED',ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE + DELF = -1 + RETURN + END IF + RETURN + 100 CONTINUE + DELF = -3 + RETURN + 200 CONTINUE + DELF = -2 + END +C ROUTINES TO CONTROL CHARACTER LOOKUP +C FOR BOTH DNA AND PROTEIN SEQUENCES +C THE INITIALISING ROUTINES ARE SENT THE CHARACTERSET SIZE IDM +C WHICH DETERMINES WHICH CHARACTERSET IS USED + SUBROUTINE INITLU(IDM) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255),POINT2(0:255) + CHARACTER DUP*16,DLOW*16,PUP*26,PLOW*26 + COMMON /IASCI1/POINT1 + COMMON /IASCI2/POINT2 + SAVE /IASCI1/ + SAVE /IASCI2/ + SAVE DUP,PUP,DLOW,PLOW + DATA DUP/'TCAG-RYWSMKHBVDN'/ + DATA PUP/'CSTPAGNDEQBZHRKMILVFYW-X? '/ + DATA DLOW/'tcag-rywsmkhbvdn'/ + DATA PLOW/'cstpagndeqbzhrkmilvfyw-x? '/ +C ICHAR RETURNS THE COLLATING SEQUENCE NUMBER +C I WANT 1-5 FOR ACGT OR 1-26 FOR AMINO ACIDS BY USING ICHAR. +C THE ACTUAL VALUE RETURNED BY ICHAR IS NOT PORTABLE +C SO I NEED TO INITIALIZE POINTR SO THAT THE CORRECT +C ELEMENTS CONTAIN VALUES 1 - 5, OR 1 - 26 +C WORKS ON UPPER AND LOWER CASE - REMOVE DLOW,PLOW AND LOOPS 41 AND 51 +C IF LOWERCASE NOT ALLOWED +C + IF(IDM.EQ.5)THEN + DO 30 I = 0,255 + POINT1(I) = IDM + POINT2(I) = 17 +30 CONTINUE + DO 35 I = 1,5 + J = ICHAR(DUP(I:I)) + POINT1(J) = I +35 CONTINUE + DO 36 I = 1,5 + J = ICHAR(DLOW(I:I)) + POINT1(J) = I +36 CONTINUE + DO 40 I = 1,16 + J = ICHAR(DUP(I:I)) + POINT2(J) = I +40 CONTINUE +C DEAL WITH U + J = ICHAR('U') + POINT1(J) = 1 + POINT2(J) = 1 + DO 41 I = 1,16 + J = ICHAR(DLOW(I:I)) + POINT2(J) = I +41 CONTINUE +C DEAL WITH U + J = ICHAR('u') + POINT1(J) = 1 + POINT2(J) = 1 + ELSE IF(IDM.EQ.26)THEN + DO 45 I = 0,255 + POINT1(I) = IDM +45 CONTINUE +C + DO 50 I = 1,26 + J = ICHAR(PUP(I:I)) + POINT1(J) = I +50 CONTINUE + DO 51 I = 1,26 + J = ICHAR(PLOW(I:I)) + POINT1(J) = I +51 CONTINUE + DO 60 I = 0,255 + POINT2(I) = POINT1(I) +60 CONTINUE + ELSE + WRITE(*,*)'ERROR INITIALISING CHARACTER LOOKUP POINTERS' + END IF + END + INTEGER FUNCTION IFROMP(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255) + CHARACTER CHAR + COMMON /IASCI1/POINT1 + SAVE /IASCI1/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + IFROMP = POINT1(ICOL) + END + INTEGER FUNCTION CTONUM(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255) + CHARACTER CHAR + COMMON /IASCI1/POINT1 + SAVE /IASCI1/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + CTONUM = POINT1(ICOL) + END + INTEGER FUNCTION DTONUM(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT2(0:255) + CHARACTER CHAR + COMMON /IASCI2/POINT2 + SAVE /IASCI2/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + DTONUM = POINT2(ICOL) + END + INTEGER FUNCTION IUBM(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A DEFINITE MATCH, 2 FOR POSSIBLE, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,2,0,2,2,0,0,2,2,2,0,2,2,0, + +0,1,0,0,2,0,2,0,2,2,0,2,2,2,0,2,0, + +0,0,1,0,2,2,0,2,0,2,0,2,0,2,2,2,0, + +0,0,0,1,2,2,0,0,2,0,2,0,2,2,2,2,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,2,1,0,2,2,2,2,2,2,2,2,2,0, + +1,1,0,0,2,0,1,2,2,2,2,2,2,2,2,2,0, + +1,0,1,0,2,2,2,1,0,2,2,2,2,2,2,2,0, + +0,1,0,1,2,2,2,0,1,2,2,2,2,2,2,2,0, + +0,1,1,0,2,2,2,2,2,1,0,2,2,2,2,2,0, + +1,0,0,1,2,2,2,2,2,2,1,2,2,2,2,2,0, + +1,1,1,0,2,2,2,1,2,1,2,1,2,2,2,2,0, + +1,1,0,1,2,2,1,2,1,2,1,2,1,2,2,2,0, + +0,1,1,1,2,1,2,2,1,1,2,2,2,1,2,2,0, + +1,0,1,1,2,1,2,1,2,2,1,2,2,2,1,2,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END + INTEGER FUNCTION IUBM2(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A POSSIBLE MATCH, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,1,0,1,1,0,0,1,1,1,0,1,1,0, + +0,1,0,0,1,0,1,0,1,1,0,1,1,1,0,1,0, + +0,0,1,0,1,1,0,1,0,1,0,1,0,1,1,1,0, + +0,0,0,1,1,1,0,0,1,0,1,0,1,1,1,1,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,1,1,0,1,1,1,1,1,1,1,1,1,0, + +1,1,0,0,1,0,1,1,1,1,1,1,1,1,1,1,0, + +1,0,1,0,1,1,1,1,0,1,1,1,1,1,1,1,0, + +0,1,0,1,1,1,1,0,1,1,1,1,1,1,1,1,0, + +0,1,1,0,1,1,1,1,1,1,0,1,1,1,1,1,0, + +1,0,0,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,1,0,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,0,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +0,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,0,1,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM2 = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END + INTEGER FUNCTION IUBM1(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A DEFINITE MATCH, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,0,1,0,0,0,0,0,0,0,0,0,0,0, + +1,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0,0, + +1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0, + +0,1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0, + +0,1,1,0,0,0,0,0,0,1,0,0,0,0,0,0,0, + +1,0,0,1,0,0,0,0,0,0,1,0,0,0,0,0,0, + +1,1,1,0,0,0,0,1,0,1,0,1,0,0,0,0,0, + +1,1,0,1,0,0,1,0,1,0,1,0,1,0,0,0,0, + +0,1,1,1,0,1,0,0,1,1,0,0,0,1,0,0,0, + +1,0,1,1,0,1,0,1,0,0,1,0,0,0,1,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM1 = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END +C +C + SUBROUTINE FLUSHL(IDEV) + CALL FLUSH(IDEV) +C This implementation uses the Sun Fortran FLUSH statement. + END + SUBROUTINE FLUSHO +C The (single) unit number is currently hard-wired, but must +C be the same as KBOUT in UNITNO. + CALL FLUSHL(6) + END +C + SUBROUTINE CEDIT(FILNAM) + CHARACTER COMAND*256,EDITOR*256,FILNAM*(*) + INTEGER SYSTEM,I + EXTERNAL NOTRL + CALL GETENV('SEQEDT',EDITOR) + I=NOTRL(EDITOR,LEN(EDITOR),' ') + IF (I.EQ.0) THEN + EDITOR='vi' + I=2 + ENDIF + COMAND = EDITOR(1:I)//' '//FILNAM + ISTAT = SYSTEM(COMAND) + END + SUBROUTINE BSW2(IN,OUT) +C byte order on cd is least significant first +C on some machines this routine should be changed to do nothing + CHARACTER*2 IN,OUT +C Sun + OUT(1:1) = IN(2:2) + OUT(2:2) = IN(1:2) +C Alliant +C OUT = IN + END + SUBROUTINE BSW4(IN,OUT) +C byte order on cd is least significant first +C on some machines this routine should be changed to do nothing + CHARACTER IN*4,OUT*4 +C Sun + DO 10 I = 1,2 + OUT(I:I) = IN(5-I:5-I) + OUT(5-I:5-I) = IN(I:I) + 10 CONTINUE +C Alliant +C OUT = IN + END + INTEGER FUNCTION IANDRS(IIN,JIN) +C AUTHOR: RODGER STADEN +C LOGICAL LIIN,LJIN +C INTEGER IN,JN +C EQUIVALENCE (LIIN,IN),(LJIN,JN) +******* PERFORMS LOGICAL OPERATIONS ON INTEGER VARIABLES +******* SO COULD BE FLAGGED AS ILLEGAL OR GIVE INCORRECT RESULTS +******* ON MANY MACHINES CAN BE REPLACED BY LOCAL INTRINSIC IAND +C IN=IIN +C JN=JIN +C LIIN=LIIN.AND.LJIN +C dec + IANDRS=IAND(IIN,JIN) +C Alliant +C IANDRS=IAND(IIN,JIN) + END + INTEGER FUNCTION IORRS(IIN,JIN) +C AUTHOR: RODGER STADEN +C LOGICAL LIIN,LJIN +C INTEGER IN,JN +C EQUIVALENCE (LIIN,IN),(LJIN,JN) +******* PERFORMS LOGICAL OPERATIONS ON INTEGER VARIABLES +******* SO COULD BE FLAGGED AS ILLEGAL OR GIVE INCORRECT RESULTS +******* ON MANY MACHINES CAN BE REPLACED BY LOCAL INTRINSIC IOR +C IN=IIN +C JN=JIN +C LIIN=LIIN.OR.LJIN +C dec + IORRS=IOR(IIN,JIN) +C Alliant +C IORRS=IOR(IIN,JIN) + END + SUBROUTINE WRITEB(CHARS,NCHAR,IDEVGR) +C AUTHOR: RODGER STADEN + CHARACTER CHARS*(*) + SAVE NUL + DATA NUL/0/ +C NOTE NULS STOP CARRIAGE RETURN, LINE FEED +C Sun + WRITE(IDEVGR,1000)CHARS(1:NCHAR) +1000 FORMAT(A,$) +C Alliant +C WRITE(IDEVGR,1000)NUL,CHARS(1:NCHAR) +C1000 FORMAT(A1,A) + END + SUBROUTINE SABIT(ARRAY,MAXAR,POSN) + INTEGER ARRAY(0:MAXAR),POSN,ELEMNT,BITNUM +C +C set bit corresponding to posn in array (bits 0-31 per word) +C + I = POSN - 1 + ELEMNT = I / 32 + BITNUM = MOD(I,32) +C WRITE(*,*)'ELEMENT, BIT',ELEMNT,BITNUM + CALL SBITRS(ARRAY(ELEMNT),BITNUM) + END + SUBROUTINE AAWORD(ARRAY1,ARRAY2,MAXAR) + INTEGER ARRAY1(0:MAXAR),ARRAY2(0:MAXAR) + INTEGER IANDRS + EXTERNAL IANDRS +C +C and array1 and array2 and put result in array1 +C + DO 10 I=0,MAXAR + ARRAY1(I) = IANDRS(ARRAY1(I),ARRAY2(I)) + 10 CONTINUE + END + SUBROUTINE OAWORD(ARRAY1,ARRAY2,MAXAR) + INTEGER ARRAY1(0:MAXAR),ARRAY2(0:MAXAR) + INTEGER IORRS + EXTERNAL IORRS +C +C OR array1 and array2 and put result in array1 +C + DO 10 I=0,MAXAR + ARRAY1(I) = IORRS(ARRAY1(I),ARRAY2(I)) + 10 CONTINUE + END + LOGICAL FUNCTION TABIT(ARRAY,MAXAR,POSN) + INTEGER ARRAY(0:MAXAR),POSN,ELEMNT,BIT + LOGICAL TBITRS + EXTERNAL TBITRS +C +C test bit corresponding to posn in array (bits 0-31 per word) +C + I = POSN - 1 + ELEMNT = I / 32 + BIT = MOD(I,32) +C WRITE(*,*)'TEST ELEMENT, BIT',ELEMNT,BIT + TABIT = .FALSE. + IF (ARRAY(ELEMNT).NE.0) THEN + TABIT = TBITRS(ARRAY(ELEMNT),BIT) + END IF + END + SUBROUTINE CABIT(ARRAY,MAXAR) + INTEGER ARRAY(0:MAXAR),POSN +C +C clear all bits in array (1 word at a time) +C + DO 10 POSN=0,MAXAR + ARRAY(POSN) = 0 + 10 CONTINUE + END + SUBROUTINE SBITRS(WORD,BITNUM) + INTEGER MASKS(0:31),WORD,BITNUM + EXTERNAL IORRS + SAVE MASKS + DATA MASKS/1,2,4,8,16,32,64,128,256,512,1024,2048,4096, + +8192,16384,32768,65536,131072,262144,524288,1048576, + +2097152,4194304,8388608,16777216,33554432,67108864, + +134217728,268435456,536870912,1073741824,-2147483648/ + WORD = IORRS(WORD,MASKS(BITNUM)) + END + LOGICAL FUNCTION TBITRS(WORD,BITNUM) + INTEGER MASKS(0:31),WORD,BITNUM + EXTERNAL IANDRS + SAVE MASKS + DATA MASKS/1,2,4,8,16,32,64,128,256,512,1024,2048,4096, + +8192,16384,32768,65536,131072,262144,524288,1048576, + +2097152,4194304,8388608,16777216,33554432,67108864, + +134217728,268435456,536870912,1073741824,-2147483648/ + I = MASKS(BITNUM) + TBITRS = (IANDRS(WORD,I).EQ.I) + END + INTEGER FUNCTION GETRS(IDEV,STRING,NSTRNG,IBYTE) + CHARACTER STRING*(*) +CRS_YUK +C CHARACTER ST + INTEGER FTELL + EXTERNAL FTELL + IREC = IBYTE + DO 10 I = 1,NSTRNG + READ(IDEV,REC=IREC,ERR=20)STRING(I:I) +CRS_YUK-START +C READ(IDEV,REC=IREC)st +C STRING(I:I) = st +C WRITE(*,*)IREC,STRING(I:I),FTELL(IDEV) +CRS_YUK-END + IREC = IREC + 1 + 10 CONTINUE + GETRS = 0 + RETURN + 20 CONTINUE + GETRS = -1 + END + INTEGER FUNCTION GETRSL(IDEV,STRING,NSTRNG) + CHARACTER STRING*(*) + CHARACTER CR + PARAMETER (CR=CHAR(13)) + READ(IDEV,1000,ERR=100,END=200)STRING(1:NSTRNG) + 1000 FORMAT(A) + DO 10 I=1,NSTRNG + IF(STRING(I:I).EQ.CR) THEN + GETRSL = I-1 + RETURN + END IF + 10 CONTINUE + GETRSL = NSTRNG + RETURN + 100 CONTINUE + WRITE(*,*)'ERROR READING IN GETRSL' + GETRSL = -2 + RETURN + 200 CONTINUE + GETRSL = -1 + WRITE(*,*)'END IN GETRSL' + END + INTEGER FUNCTION SEEKRS(IDEV,IREC) + INTEGER FSEEK + SEEKRS = FSEEK(IDEV,IREC,0) + END diff --git a/src/staden/seeme.f b/src/staden/seeme.f new file mode 100644 index 0000000..9e35d7a --- /dev/null +++ b/src/staden/seeme.f @@ -0,0 +1,705 @@ +C SEEME: MACHINE SPECIFIC ROUTINES +C 14-8-91 Added check in openrs for empty file names +C 27-8-91 Started files xspec.f and nxspec.f and split out the nonx +C and X specific bits from here. +C 27-8-91 Added FLUSHL to flush unit idev +C 15-10-91 Moved IANDRS, IORRS and WRITEB from pl4010.f +C 29-10-91 Replaced lnblnk ( a sun routine) by notrl +C 15-11-91 Added routines to call inquire to see if a file exists +C and another to delete a file +C 25-2-92 added more bit handling routines for use by sequence library +C searching routines +C moved getrs, getrsl and fseekrs here from seqlibsubs.f +C AUTHOR: RODGER STADEN +C 25-JAN-1990 INILU: SAVE DUP,PUP,DLOW,PLOW +C UNITNO +C OPENRS +C THE FOLLOWING ARE PROBABLY COMPLETELY PORTABLE +C BUT ARE INCLUDED ANY WAY +C INITLU +C IFROMP +C CTONUM +C DTONUM +C IUBM +C IUBM1 +C IUBM2 +C bsw4 +C bsw2 + SUBROUTINE UNITNO(KBIN,KBOUT,DEVNOS,NDEVS) +C AUTHOR: RODGER STADEN + INTEGER DEVNOS(NDEVS) +C SETS CONSOLE I/O UNITS TO 5,6. OTHERS 10 ONWARDS + KBIN=5 + KBOUT=6 + ISTART=10 + DO 1 I=1,NDEVS + DEVNOS(I)=ISTART+I-1 +1 CONTINUE + CALL INITRS() + END + SUBROUTINE OPENRS(IDEV,FN,IOK,LRECL,JOB) +C AUTHOR: RODGER STADEN + CHARACTER FN*(*) + CHARACTER*1024 FILNAM,FNDFIL + EXTERNAL FNDFIL,NOTRL,INQF +C 14-8-91 Added check for blank file names +C 14-11-91 Added routines to check for file existence +C ROUTINE TO OPEN FILES +C JOB=1 SEQUENTIAL FORMATTED NEW, DEFAULT RECORD LENGTH +C =2 SEQUENTIAL FORMATTED OLD, DEFAULT RECORD LENGTH +C =3 DIRECT ACCESS, UNFORMATTED NEW +C =4 DIRECT ACCESS, UNFORMATTED OLD +C =5 DIRECT ACCESS, UNFORMATTED OLD +C =6 UNFORMATTED NEW +C =7 UNFORMATTED OLD +C =8 STATUS='UNKNOWN' +C =9 TERMINAL FOR GRAPHICS +C =10 TERMINAL FOR VT100 +C =11 direct access, single byte record length, old +C RECORD LENGTHS SENT IN WORDS +C READONLY IS VAX SPECIFIC********************* +C NOTE THAT ONLY FOR DIRECT ACCESS FILES ARE RECORD +C LENGTHS SPECIFIED. ON THE VAX UNFORMATTED DIRECT ACCESS +C RECORDS ARE DEFINED IN WORDS; ON OTHER MACHINES E.G. SOME UNIX +C MACHINES THEY ARE SPECIFIED IN BYTES. THE VARIABLE IWORDL IS +C USED TO MULTIPLY RECORD LENGTHS SPECIFIED IN WORDS TO GIVE +C THE CORRECT VALUE FOR THE MACHINE. ON A VAX IWORDL=1; ON +C THE UNIX MACHINE IWORDL=4 + PARAMETER (IWORDL=4) + IF(NOTRL(FN,LEN(FN),' ').EQ.0) GO TO 100 + IF (JOB.LT.9 .OR. JOB.GT.10) THEN + FILNAM = FNDFIL(FN) + INQJ = INQF(FILNAM) + END IF + IF(JOB.EQ.1) THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + ACCESS='SEQUENTIAL',ERR=100) + ELSE IF(JOB.EQ.2)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='SEQUENTIAL',ERR=100) + ELSE IF(JOB.EQ.3)THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.4)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.5)THEN + JRECL=LRECL*IWORDL + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + ELSE IF(JOB.EQ.6)THEN + IF(INQJ.NE.0) THEN +C file exists so tell the user + IOK = 2 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='NEW', + + FORM='UNFORMATTED',ERR=100) + ELSE IF(JOB.EQ.7)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + FORM='UNFORMATTED',ERR=100) + ELSE IF(JOB.EQ.8)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.9)THEN +C DEVICE FOR GRAPHICS OUTPUT IS 'TT' IF USING A +C VT640 ON A VAX. IF USING A SEPARATE TERMINAL FOR +C GRAPHICS OUTPUT THE TERMINAL PROTECTION MUST BE +C SET W:RW AND THE DEVICE WILL HAVE A DIFFERENT NAME +C FOR EXAMPLE 'GRAPHICS' WHERE THE LOGICAL NAME 'GRAPHICS' +C IS ASSIGNED TO THE APPROPRIATE DEVICE. A VT240 SHOULD +C BE COMPATIBLE WITH THE TEKTRONIX PLOT COMMANDS. + OPEN(UNIT=IDEV,FILE='/dev/tty',STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.10)THEN +C DEVICE FOR VT100 COMPATIBLE TERMINAL WHEN USED +C FOR GELIN WHICH REQUIRES COMPLETE CONTROL OF +C CURSOR POSITIONING. ON A VAX IT IS CALLED 'TT' + OPEN(UNIT=IDEV,FILE='/dev/tty',STATUS='UNKNOWN', + + ERR=100) + ELSE IF(JOB.EQ.11)THEN + IF(INQJ.NE.1) THEN +C file inquire shows file does not exist +C so tell the user + IOK = 3 + RETURN + END IF + JRECL=1 + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + END IF + IOK=0 + RETURN +100 CONTINUE + IOK=1 + RETURN + END + CHARACTER*1024 FUNCTION FNDFIL(NAME) + CHARACTER NAME*(*),TMPNAM*1024,PREFIX*1024 + EXTERNAL NOTRL +C LOOKS IN ENVIRONMENT FOR FILE PATHS +C ? CASES DEALT WITH: +C 1) IF NAME STARTS WITH / IT IS ABSOLUTE AND SO UNCHANGED +C 2) IF NAME STARTS WITH ~/ REPLACE WITH HOME DIRECTORY NAME +C 3) IF NAME INCLUDES ANY OTHER / LOOK IN ENVIRONMENT FOR +C NAME UPTO /, AND ADD PATH TO REST IF FOUND +C IF NOT FOUND ADD PATH TO CURRENT WORKING DIRECTORY +C 4) ELSE LOOK IN ENVIRONMENT FOR WHOLE NAME +C IF NOT FOUND ADD PATH TO CURRENT DIRECTORY +C IF FOUND MAKE IT FILE NAME +C + IF(NAME(1:1).EQ.'/') THEN + FNDFIL = NAME + ELSE IF (NAME(1:2).EQ.'~/') THEN + CALL GETENV('HOME',TMPNAM) + FNDFIL = TMPNAM(:NOTRL(TMPNAM,LEN(TMPNAM),' ')) // + + NAME(2:NOTRL(NAME,LEN(NAME),' ')) + ELSE IF (INDEX(NAME,'/').NE.0) THEN + TMPNAM = NAME(1:INDEX(NAME,'/')-1) + CALL GETENV(TMPNAM,PREFIX) + I = NOTRL(PREFIX,LEN(PREFIX),' ') + IF(I.EQ.0) THEN + CALL GETCWD(PREFIX) + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + '/' // NAME(:NOTRL(NAME,LEN(NAME),' ')) + ELSE + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + NAME(INDEX(NAME,'/'):) + END IF + ELSE + CALL GETENV(NAME,TMPNAM) + I = NOTRL(TMPNAM,LEN(TMPNAM),' ') + IF (I.EQ.0) THEN + CALL GETCWD(PREFIX) + FNDFIL = PREFIX(:NOTRL(PREFIX,LEN(PREFIX),' ')) // + + '/' // NAME(:NOTRL(NAME,LEN(NAME),' ')) + ELSE + FNDFIL = TMPNAM + END IF + END IF + END + INTEGER FUNCTION INQF(NAME) + CHARACTER NAME*(*) + LOGICAL EX + INQUIRE(FILE=NAME,EXIST=EX) +C +C if the file exists return 1, else 0 +C + IF (EX) THEN + INQF = 1 + ELSE + INQF = 0 + END IF + END + INTEGER FUNCTION DELF(FN,IDEV,JRECL,JOB) + CHARACTER FN*(*) + CHARACTER FILNAM*1024,FNDFIL*1024 + EXTERNAL FNDFIL +C +C routine to delete files in the most horrible way i could find +C deal with job 2: old sequential formatted +C deal with job 4: old direct unformatted +C deal with job 7: old sequential unformatted +C if the file exists and is not one of these error =-1 +C if the file exists and cannot be deleted error =-2 +C if the file cannot be opened error =-3 +C +C on the sun this technique will delete any file if the directory +C priviliges are set. +C I dont know if i need to worry about the file types: could +C i just open them all the same way? Although its a mess leave it! + FILNAM = FNDFIL(FN) + IF(JOB.EQ.2) THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='SEQUENTIAL',ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE IF(JOB.EQ.4)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + ACCESS='DIRECT',RECL=JRECL,ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE IF(JOB.EQ.7)THEN + OPEN(UNIT=IDEV,FILE=FILNAM,STATUS='OLD', + + FORM='UNFORMATTED',ERR=100) + CLOSE(UNIT=IDEV,STATUS='DELETE',ERR=200) + DELF = 0 + ELSE + DELF = -1 + RETURN + END IF + RETURN + 100 CONTINUE + DELF = -3 + RETURN + 200 CONTINUE + DELF = -2 + END +C ROUTINES TO CONTROL CHARACTER LOOKUP +C FOR BOTH DNA AND PROTEIN SEQUENCES +C THE INITIALISING ROUTINES ARE SENT THE CHARACTERSET SIZE IDM +C WHICH DETERMINES WHICH CHARACTERSET IS USED + SUBROUTINE INITLU(IDM) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255),POINT2(0:255) + CHARACTER DUP*16,DLOW*16,PUP*26,PLOW*26 + COMMON /IASCI1/POINT1 + COMMON /IASCI2/POINT2 + SAVE /IASCI1/ + SAVE /IASCI2/ + SAVE DUP,PUP,DLOW,PLOW + DATA DUP/'TCAG-RYWSMKHBVDN'/ + DATA PUP/'CSTPAGNDEQBZHRKMILVFYW-X? '/ + DATA DLOW/'tcag-rywsmkhbvdn'/ + DATA PLOW/'cstpagndeqbzhrkmilvfyw-x? '/ +C ICHAR RETURNS THE COLLATING SEQUENCE NUMBER +C I WANT 1-5 FOR ACGT OR 1-26 FOR AMINO ACIDS BY USING ICHAR. +C THE ACTUAL VALUE RETURNED BY ICHAR IS NOT PORTABLE +C SO I NEED TO INITIALIZE POINTR SO THAT THE CORRECT +C ELEMENTS CONTAIN VALUES 1 - 5, OR 1 - 26 +C WORKS ON UPPER AND LOWER CASE - REMOVE DLOW,PLOW AND LOOPS 41 AND 51 +C IF LOWERCASE NOT ALLOWED +C + IF(IDM.EQ.5)THEN + DO 30 I = 0,255 + POINT1(I) = IDM + POINT2(I) = 17 +30 CONTINUE + DO 35 I = 1,5 + J = ICHAR(DUP(I:I)) + POINT1(J) = I +35 CONTINUE + DO 36 I = 1,5 + J = ICHAR(DLOW(I:I)) + POINT1(J) = I +36 CONTINUE + DO 40 I = 1,16 + J = ICHAR(DUP(I:I)) + POINT2(J) = I +40 CONTINUE +C DEAL WITH U + J = ICHAR('U') + POINT1(J) = 1 + POINT2(J) = 1 + DO 41 I = 1,16 + J = ICHAR(DLOW(I:I)) + POINT2(J) = I +41 CONTINUE +C DEAL WITH U + J = ICHAR('u') + POINT1(J) = 1 + POINT2(J) = 1 + ELSE IF(IDM.EQ.26)THEN + DO 45 I = 0,255 + POINT1(I) = IDM +45 CONTINUE +C + DO 50 I = 1,26 + J = ICHAR(PUP(I:I)) + POINT1(J) = I +50 CONTINUE + DO 51 I = 1,26 + J = ICHAR(PLOW(I:I)) + POINT1(J) = I +51 CONTINUE + DO 60 I = 0,255 + POINT2(I) = POINT1(I) +60 CONTINUE + ELSE + WRITE(*,*)'ERROR INITIALISING CHARACTER LOOKUP POINTERS' + END IF + END + INTEGER FUNCTION IFROMP(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255) + CHARACTER CHAR + COMMON /IASCI1/POINT1 + SAVE /IASCI1/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + IFROMP = POINT1(ICOL) + END + INTEGER FUNCTION CTONUM(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT1(0:255) + CHARACTER CHAR + COMMON /IASCI1/POINT1 + SAVE /IASCI1/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + CTONUM = POINT1(ICOL) + END + INTEGER FUNCTION DTONUM(CHAR) +C AUTHOR RODGER STADEN + INTEGER POINT2(0:255) + CHARACTER CHAR + COMMON /IASCI2/POINT2 + SAVE /IASCI2/ +C +C GET COLLATING SEQUENCE VALUE + ICOL = ICHAR(CHAR) +C THIS POINTS TO A VALUE IN POINTR + DTONUM = POINT2(ICOL) + END + INTEGER FUNCTION IUBM(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A DEFINITE MATCH, 2 FOR POSSIBLE, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,2,0,2,2,0,0,2,2,2,0,2,2,0, + +0,1,0,0,2,0,2,0,2,2,0,2,2,2,0,2,0, + +0,0,1,0,2,2,0,2,0,2,0,2,0,2,2,2,0, + +0,0,0,1,2,2,0,0,2,0,2,0,2,2,2,2,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,2,1,0,2,2,2,2,2,2,2,2,2,0, + +1,1,0,0,2,0,1,2,2,2,2,2,2,2,2,2,0, + +1,0,1,0,2,2,2,1,0,2,2,2,2,2,2,2,0, + +0,1,0,1,2,2,2,0,1,2,2,2,2,2,2,2,0, + +0,1,1,0,2,2,2,2,2,1,0,2,2,2,2,2,0, + +1,0,0,1,2,2,2,2,2,2,1,2,2,2,2,2,0, + +1,1,1,0,2,2,2,1,2,1,2,1,2,2,2,2,0, + +1,1,0,1,2,2,1,2,1,2,1,2,1,2,2,2,0, + +0,1,1,1,2,1,2,2,1,1,2,2,2,1,2,2,0, + +1,0,1,1,2,1,2,1,2,2,1,2,2,2,1,2,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END + INTEGER FUNCTION IUBM2(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A POSSIBLE MATCH, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,1,0,1,1,0,0,1,1,1,0,1,1,0, + +0,1,0,0,1,0,1,0,1,1,0,1,1,1,0,1,0, + +0,0,1,0,1,1,0,1,0,1,0,1,0,1,1,1,0, + +0,0,0,1,1,1,0,0,1,0,1,0,1,1,1,1,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,1,1,0,1,1,1,1,1,1,1,1,1,0, + +1,1,0,0,1,0,1,1,1,1,1,1,1,1,1,1,0, + +1,0,1,0,1,1,1,1,0,1,1,1,1,1,1,1,0, + +0,1,0,1,1,1,1,0,1,1,1,1,1,1,1,1,0, + +0,1,1,0,1,1,1,1,1,1,0,1,1,1,1,1,0, + +1,0,0,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,1,0,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,0,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +0,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,0,1,1,1,1,1,1,1,1,1,1,1,1,1,1,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM2 = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END + INTEGER FUNCTION IUBM1(SEQ,ENZ) +C AUTHOR: RODGER STADEN +C RETURNS 1 FOR A DEFINITE MATCH, ELSE 0 + CHARACTER SEQ,ENZ + INTEGER TABLE(17,17),DTONUM + EXTERNAL DTONUM + DATA TABLE/ + +1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0,0, + +0,0,0,1,0,0,0,0,0,0,0,0,0,0,0,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,1,1,0,1,0,0,0,0,0,0,0,0,0,0,0, + +1,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0,0, + +1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0,0, + +0,1,0,1,0,0,0,0,1,0,0,0,0,0,0,0,0, + +0,1,1,0,0,0,0,0,0,1,0,0,0,0,0,0,0, + +1,0,0,1,0,0,0,0,0,0,1,0,0,0,0,0,0, + +1,1,1,0,0,0,0,1,0,1,0,1,0,0,0,0,0, + +1,1,0,1,0,0,1,0,1,0,1,0,1,0,0,0,0, + +0,1,1,1,0,1,0,0,1,1,0,0,0,1,0,0,0, + +1,0,1,1,0,1,0,1,0,0,1,0,0,0,1,0,0, + +1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1,1, + +0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0,0/ +C + IUBM1 = TABLE(DTONUM(ENZ),DTONUM(SEQ)) + END +C +C + SUBROUTINE FLUSHL(IDEV) + CALL FLUSH(IDEV) +C This implementation uses the Sun Fortran FLUSH statement. + END + SUBROUTINE FLUSHO +C The (single) unit number is currently hard-wired, but must +C be the same as KBOUT in UNITNO. + CALL FLUSHL(6) + END +C + SUBROUTINE CEDIT(FILNAM) + CHARACTER COMAND*256,EDITOR*256,FILNAM*(*) + INTEGER SYSTEM,I + EXTERNAL NOTRL + CALL GETENV('SEQEDT',EDITOR) + I=NOTRL(EDITOR,LEN(EDITOR),' ') + IF (I.EQ.0) THEN + EDITOR='vi' + I=2 + ENDIF + COMAND = EDITOR(1:I)//' '//FILNAM + ISTAT = SYSTEM(COMAND) + END + SUBROUTINE BSW2(IN,OUT) +C byte order on cd is least significant first +C on some machines this routine should be changed to do nothing + CHARACTER*2 IN,OUT +C Sun + OUT(1:1) = IN(2:2) + OUT(2:2) = IN(1:2) +C Alliant +C OUT = IN + END + SUBROUTINE BSW4(IN,OUT) +C byte order on cd is least significant first +C on some machines this routine should be changed to do nothing + CHARACTER IN*4,OUT*4 +C Sun + DO 10 I = 1,2 + OUT(I:I) = IN(5-I:5-I) + OUT(5-I:5-I) = IN(I:I) + 10 CONTINUE +C Alliant +C OUT = IN + END + INTEGER FUNCTION IANDRS(IIN,JIN) +C AUTHOR: RODGER STADEN +C LOGICAL LIIN,LJIN +C INTEGER IN,JN +C EQUIVALENCE (LIIN,IN),(LJIN,JN) +******* PERFORMS LOGICAL OPERATIONS ON INTEGER VARIABLES +******* SO COULD BE FLAGGED AS ILLEGAL OR GIVE INCORRECT RESULTS +******* ON MANY MACHINES CAN BE REPLACED BY LOCAL INTRINSIC IAND +C IN=IIN +C JN=JIN +C LIIN=LIIN.AND.LJIN +C Sun + IANDRS=AND(IIN,JIN) +C Alliant +C IANDRS=IAND(IIN,JIN) + END + INTEGER FUNCTION IORRS(IIN,JIN) +C AUTHOR: RODGER STADEN +C LOGICAL LIIN,LJIN +C INTEGER IN,JN +C EQUIVALENCE (LIIN,IN),(LJIN,JN) +******* PERFORMS LOGICAL OPERATIONS ON INTEGER VARIABLES +******* SO COULD BE FLAGGED AS ILLEGAL OR GIVE INCORRECT RESULTS +******* ON MANY MACHINES CAN BE REPLACED BY LOCAL INTRINSIC IOR +C IN=IIN +C JN=JIN +C LIIN=LIIN.OR.LJIN +C Sun + IORRS=OR(IIN,JIN) +C Alliant +C IORRS=IOR(IIN,JIN) + END + SUBROUTINE WRITEB(CHARS,NCHAR,IDEVGR) +C AUTHOR: RODGER STADEN + CHARACTER CHARS*(*) + SAVE NUL + DATA NUL/0/ +C NOTE NULS STOP CARRIAGE RETURN, LINE FEED +C Sun + WRITE(IDEVGR,1000)CHARS(1:NCHAR) +1000 FORMAT(A,$) +C Alliant +C WRITE(IDEVGR,1000)NUL,CHARS(1:NCHAR) +C1000 FORMAT(A1,A) + END + SUBROUTINE SABIT(ARRAY,MAXAR,POSN) + INTEGER ARRAY(0:MAXAR),POSN,ELEMNT,BITNUM +C +C set bit corresponding to posn in array (bits 0-31 per word) +C + I = POSN - 1 + ELEMNT = I / 32 + BITNUM = MOD(I,32) +C WRITE(*,*)'ELEMENT, BIT',ELEMNT,BITNUM + CALL SBITRS(ARRAY(ELEMNT),BITNUM) + END + SUBROUTINE AAWORD(ARRAY1,ARRAY2,MAXAR) + INTEGER ARRAY1(0:MAXAR),ARRAY2(0:MAXAR) + INTEGER IANDRS + EXTERNAL IANDRS +C +C and array1 and array2 and put result in array1 +C + DO 10 I=0,MAXAR + ARRAY1(I) = IANDRS(ARRAY1(I),ARRAY2(I)) + 10 CONTINUE + END + SUBROUTINE OAWORD(ARRAY1,ARRAY2,MAXAR) + INTEGER ARRAY1(0:MAXAR),ARRAY2(0:MAXAR) + INTEGER IORRS + EXTERNAL IORRS +C +C OR array1 and array2 and put result in array1 +C + DO 10 I=0,MAXAR + ARRAY1(I) = IORRS(ARRAY1(I),ARRAY2(I)) + 10 CONTINUE + END + LOGICAL FUNCTION TABIT(ARRAY,MAXAR,POSN) + INTEGER ARRAY(0:MAXAR),POSN,ELEMNT,BIT + LOGICAL TBITRS + EXTERNAL TBITRS +C +C test bit corresponding to posn in array (bits 0-31 per word) +C + I = POSN - 1 + ELEMNT = I / 32 + BIT = MOD(I,32) +C WRITE(*,*)'TEST ELEMENT, BIT',ELEMNT,BIT + TABIT = TBITRS(ARRAY(ELEMNT),BIT) + END + SUBROUTINE CABIT(ARRAY,MAXAR) + INTEGER ARRAY(0:MAXAR),POSN +C +C clear all bits in array (1 at a time!!!!!!!!) +C + DO 10 POSN=0,MAXAR + DO 5 J = 0,31 + CALL CBITRS(ARRAY(POSN),J) + 5 CONTINUE + 10 CONTINUE + END + SUBROUTINE TBITA(ARRAY,MAXAR,IDEV) + INTEGER ARRAY(0:MAXAR) + LOGICAL TBITRS + EXTERNAL TBITRS +C +C test all bits in array to see if they are set +C + DO 10 I=0,MAXAR + DO 5 J=0,31 + IF(TBITRS(ARRAY(I),J)) THEN + K = I*32 + 1 + MOD(J,32) + WRITE(IDEV,*)' BIT SET FOR POSN',K + END IF + 5 CONTINUE + 10 CONTINUE + END +C routines to handle bits (machine specific) for use when +C searching through keyword indexes +C +C positions for marking are numbered from 1 onwards +C but array elements and bits are numbered from 0 onwards +C + SUBROUTINE SBITRS(WORD,BITNUM) +C +C set bit bitnum in word +C + INTEGER WORD,BITNUM +C alliant +C INTEGER IBSET +C INTRINSIC IBSET +C WORD = IBSET(WORD,BITNUM) +C sun + CALL BIS(BITNUM,WORD) + END + SUBROUTINE CBITRS(WORD,BITNUM) +C +C clear bit bitnum in word +C + INTEGER WORD,BITNUM +C alliant +C INTEGER IBCLR +C INTRINSIC IBCLR +C WORD = IBCLR(WORD,BITNUM) +C sun + CALL BIC(BITNUM,WORD) + END + LOGICAL FUNCTION TBITRS(WORD,BITNUM) +C +C test bit bitnum in word +C + INTEGER WORD,BITNUM +C alliant +C LOGICAL BTEST +C INTRINSIC BTEST +C TBITRS = BTEST(WORD,BITNUM) +C sun + LOGICAL BIT + EXTERNAL BIT + TBITRS = BIT(BITNUM,WORD) + END + INTEGER FUNCTION GETRS(IDEV,STRING,NSTRNG,IBYTE) + CHARACTER STRING*(*) + IREC = IBYTE + DO 10 I=1,NSTRNG + READ(IDEV,REC=IREC,ERR=20)STRING(I:I) + IREC = IREC + 1 + 10 CONTINUE + GETRS = 0 + RETURN + 20 CONTINUE + GETRS = -1 + END + INTEGER FUNCTION GETRSL(IDEV,STRING,NSTRNG) + CHARACTER STRING*(*) + CHARACTER CR + PARAMETER (CR=CHAR(13)) + READ(IDEV,1000,ERR=100,END=200)STRING(1:NSTRNG) + 1000 FORMAT(A) + DO 10 I=1,NSTRNG + IF(STRING(I:I).EQ.CR) THEN + GETRSL = I-1 + RETURN + END IF + 10 CONTINUE + GETRSL = NSTRNG + RETURN + 100 CONTINUE + WRITE(*,*)'ERROR READING IN GETRSL' + GETRSL = -2 + RETURN + 200 CONTINUE + GETRSL = -1 + WRITE(*,*)'END IN GETRSL' + END + INTEGER FUNCTION SEEKRS(IDEV,IREC) + INTEGER FSEEK + SEEKRS = FSEEK(IDEV,IREC,0) + END diff --git a/src/staden/select.c b/src/staden/select.c new file mode 100644 index 0000000..0effe5e --- /dev/null +++ b/src/staden/select.c @@ -0,0 +1,318 @@ +#include "edUtils.h" +#include "select.h" +#include "Sheet.h" +#include "tagUtils.h" +#include +#include + +extern enum States {StateDown=0,StateUp} editorState; + +/* ----- private subroutines ----- */ +#define left_margin 4 +#define top_margin 4 + +static void draw_select(EdStruct *xx, int seq, int from_pos, int to_pos) +{ + int *seqList; + int s_from,s_to; + int temp; + int screenRow; + + /* sort from_pos, to_pos */ + if (from_pos > to_pos) { temp = from_pos; from_pos = to_pos; to_pos = temp; } + + /* clip to screen */ + s_from = positionInContig(xx,seq,from_pos) - xx->displayPos; + if (s_from>=xx->displayWidth) return; + if (s_from<0) s_from=0; + s_to = positionInContig(xx,seq,to_pos) - xx->displayPos; + if (s_to<0) return; + if (s_to>=xx->displayWidth) s_to = xx->displayWidth-1; + + seqList = sequencesOnScreen(xx,xx->displayPos, xx->displayWidth); + for(screenRow=0; + screenRowdisplayHeight && seqList[screenRow] != seq; + screenRow++); + if (screenRow==xx->displayHeight) return; + XawSheetOpHilightText(xx->sequencesWid,s_from,screenRow+xx->rulerDisplayed,s_to-s_from+1,sh_select,HOP_TOG); + +} + +static void undoSelection(EdStruct *xx) +{ + redisplaySelection(xx); + xx->select_made = 0; +} + +static void lose_ownership_proc(Widget w, Atom *sel) +{ + EdStruct *xx = widgetToEdStruct(XtParent(w)); + undoSelection(xx); +} + +static Boolean convert_proc( + Widget w, + Atom *selection, + Atom *target, + Atom *type_return, + XtPointer *value_return, + unsigned long *length_return, + int *format_return) +{ + EdStruct *xx = widgetToEdStruct(XtParent(w)); + int temp; + + if (*target == XA_STRING ) { + int start_pos,end_pos; + start_pos = xx->select_start_pos; + end_pos = xx->select_end_pos; + if (start_pos > end_pos) { + temp = start_pos; + start_pos = end_pos; + end_pos = temp; + } + *length_return = end_pos - start_pos; + *value_return = XtMalloc(*length_return+1); + if (xx->select_seq == 0) + DBcalcConsensus(xx,start_pos,*length_return,*value_return,BOTH_STRANDS); + else + DBgetSequence(xx,xx->select_seq,start_pos-1,*length_return,*value_return); + *type_return = XA_STRING; + *format_return = 8; + return (TRUE); + } + return (FALSE); +} + +/* ----- exported subroutines ----- */ +void start_highlight (Widget w, XButtonEvent *event, String *params, + Cardinal *num_params) +{ + EdStruct *xx = widgetToEdStruct(XtParent(w)); + int x,y; + int *seqList; + + if (editorState == StateDown) return; + + if (xx->select_made) undoSelection(xx); + xx->select_made = 1; + + x = (event->x-left_margin) / xx->fontWidth; + y = (event->y-top_margin) / xx->fontHeight - xx->rulerDisplayed; + if (y<0) y=0; + if (y>=xx->displayHeight) y=xx->displayHeight-1; + if (x<0) x=0; + if (x>=xx->displayWidth) x=xx->displayWidth-1; + + seqList = sequencesOnScreen(xx,xx->displayPos,xx->displayWidth); + xx->select_seq= seqList[y]; + xx->select_start_pos = xx->displayPos - DBgetRelPos(xx,xx->select_seq) + x + 1; + if (xx->select_start_pos<1) + xx->select_start_pos = 1; + else + if (xx->select_start_pos > DBgetLength(xx,xx->select_seq)+1) + xx->select_start_pos = DBgetLength(xx,xx->select_seq)+1; + xx->select_end_pos = xx->select_start_pos; + xx->select_tag = NULL; + +} + + + +void make_selection (Widget w, XButtonEvent *event, String *params, + Cardinal *num_params) +{ + EdStruct *xx = widgetToEdStruct(XtParent(w)); + + if (XtOwnSelection(w,XA_PRIMARY,event->time,convert_proc, + lose_ownership_proc, NULL) == FALSE) { + XtWarning("Editor: failed to become selection owner; make a new selection.\n"); + undoSelection(xx); + } + +} + +void disown_selection(EdStruct *xx) +{ + if (xx->select_made) + XtDisownSelection(xx->sequencesWid,XA_PRIMARY,CurrentTime); + xx->select_made = 0; +} + +void extend_highlight (Widget w, XButtonEvent *event, String *params, + Cardinal *num_params) +{ + EdStruct *xx = widgetToEdStruct(XtParent(w)); + + int pos; + int x; + + if ( ! xx->select_made) return; + x = (event->x-left_margin) / xx->fontWidth; + if (x<0) x=0; + if (x>=xx->displayWidth) x=xx->displayWidth-1; + + pos = xx->displayPos - DBgetRelPos(xx,xx->select_seq) + x + 1; + if (pos<1) + pos = 1; + else + if (pos > DBgetLength(xx,xx->select_seq)+1) + pos = DBgetLength(xx,xx->select_seq)+1; + + if (pos == xx->select_end_pos) return; + + if (xx->select_start_pos < xx->select_end_pos) { + if (xx->select_end_pos < pos) + draw_select(xx, xx->select_seq, xx->select_end_pos,pos-1); + else + draw_select(xx, xx->select_seq, pos,xx->select_end_pos-1); + } else { + if (xx->select_end_pos > pos) + draw_select(xx, xx->select_seq, pos,xx->select_end_pos-1); + else + draw_select(xx, xx->select_seq, xx->select_end_pos,pos-1); + } + + xx->select_end_pos = pos; +} + + + +void redisplaySelection(EdStruct *xx) +{ + if (!xx->select_made) return; + if (xx->select_start_pos == xx->select_end_pos) ; + else if (xx->select_start_pos < xx->select_end_pos) { + draw_select(xx, xx->select_seq, xx->select_start_pos,xx->select_end_pos-1); + } else { + draw_select(xx, xx->select_seq, xx->select_end_pos,xx->select_start_pos-1); + } +} + + +int getSelection(EdStruct *xx, int *seq, int *start, int *length, tagStruct **t) +{ + if (! xx->select_made) return 0; + if (xx->select_start_pos <= xx->select_end_pos) { + *seq = xx->select_seq; + *start = xx->select_start_pos; + *length = xx->select_end_pos - xx->select_start_pos; + *t = xx->select_tag; + } else { + *seq = xx->select_seq; + *start = xx->select_end_pos; + *length = xx->select_start_pos - xx->select_end_pos; + *t = xx->select_tag; + } + return 1; +} + +void _select_tag(EdStruct *xx, int seq, tagStruct *t) +{ + XButtonEvent event; + if (t==NULL) return; + + if (xx->select_made) undoSelection(xx); + xx->select_made = 1; + xx->select_seq = seq; + xx->select_start_pos = normalisePos(xx,seq,t->tagrec.position,t->tagrec.length); + xx->select_end_pos = xx->select_start_pos + t->tagrec.length; + xx->select_tag = t; + + redisplaySelection(xx); + event.time = CurrentTime; + make_selection (xx->sequencesWid, &event, NULL, 0); + +} + +void select_tag(Widget w, XButtonEvent *event, String *params, + Cardinal *num_params) +{ + EdStruct *xx = widgetToEdStruct(XtParent(w)); + int x,y; + int *seqList; + int seq,pos; + tagStruct *t; + + x = (event->x-left_margin) / xx->fontWidth; + y = (event->y-top_margin) / xx->fontHeight - xx->rulerDisplayed; + if (y<0) y=0; + if (y>=xx->displayHeight) y=xx->displayHeight-1; + if (x<0) x=0; + if (x>=xx->displayWidth) x=xx->displayWidth-1; + + seqList = sequencesOnScreen(xx,xx->displayPos,xx->displayWidth); + seq = seqList[y]; + pos = xx->displayPos - DBgetRelPos(xx,seq) + x + 1; + + if ( (t=findTag(xx,seq,pos)) != NULL) { + if (xx->select_made) undoSelection(xx); + xx->select_made = 1; + xx->select_seq = seq; + xx->select_start_pos = normalisePos(xx,seq,t->tagrec.position,t->tagrec.length); + xx->select_end_pos = xx->select_start_pos + t->tagrec.length; + xx->select_tag = t; + + redisplaySelection(xx); + make_selection (w, event, NULL, 0); + } + +} + +void selectInsertBase(EdStruct *xx, int seq, int pos) +/* +** Adjust selection on insertion +*/ +{ + if (xx->select_made && xx->select_seq==seq) { + int inverted=(xx->select_end_pos < xx->select_start_pos); + int start,end; + + if (inverted) { + start=xx->select_end_pos; + end =xx->select_start_pos; + } else { + end =xx->select_end_pos; + start=xx->select_start_pos; + } + + if (pos <= start) { + xx->select_start_pos++; + xx->select_end_pos++; + } else if (pos < end) { + if (inverted) + xx->select_start_pos++; + else + xx->select_end_pos++; + } + } +} + +void selectDeleteBase(EdStruct *xx, int seq, int pos) +/* +** Adjust selection on deletion +*/ +{ + if (xx->select_made && xx->select_seq==seq) { + int inverted=(xx->select_end_pos < xx->select_start_pos); + int start,end; + + if (inverted) { + start=xx->select_end_pos; + end =xx->select_start_pos; + } else { + end =xx->select_end_pos; + start=xx->select_start_pos; + } + + if (pos < start) { + xx->select_start_pos--; + xx->select_end_pos--; + } else if (pos < end) { + if (inverted) + xx->select_start_pos--; + else + xx->select_end_pos--; + } + } +} diff --git a/src/staden/select.h b/src/staden/select.h new file mode 100644 index 0000000..728f029 --- /dev/null +++ b/src/staden/select.h @@ -0,0 +1,16 @@ +#ifndef _select_h +#define _select_h + +#include "tagUtils.h" + +extern void start_highlight (Widget, XButtonEvent *, String *, Cardinal *); +extern void make_selection (Widget, XButtonEvent *, String *, Cardinal *); +extern void extend_highlight(Widget, XButtonEvent *, String *, Cardinal *); +extern void select_tag(Widget, XButtonEvent *, String *, Cardinal *); +extern void _select_tag(EdStruct *xx, int seq, tagStruct *t); +extern void redisplaySelection(EdStruct *xx); +extern void disown_selection(EdStruct *xx); +extern int getSelection(EdStruct *xx, int *seq, int *start, int *length, tagStruct **t); +extern void selectInsertBase(EdStruct *xx, int seq, int pos); +extern void selectDeleteBase(EdStruct *xx, int seq, int pos); +#endif /* _select_h */ diff --git a/src/staden/seqlibsubs.f b/src/staden/seqlibsubs.f new file mode 100644 index 0000000..7206bd1 --- /dev/null +++ b/src/staden/seqlibsubs.f @@ -0,0 +1,3290 @@ +C seqlibsubs +C 4-5-93 Added lip routine RDLIBB and lots of diagnostics +C 17-2-93 Stopped keyword and author searches giving "error reading index" +C by changes to ikwrd and ianum +C 28-9-92 Stopped rdlibl from giving "error reading index" +C for end of library - sipl,pipl,nipl +C 17-6-92 Added routines to deal with fasta format (Not +C done very carefully!). Requires a change to library +C searching programs (sipl, pipl, nipl) to denote the +C first entry. +C 30-5-91 changed major library format to embl cd +C 1-10-91 removed references to cdseqs +C 21-10-91 added keyword index search for what it is currently worth +C and changed to new format for brief.idx files +C In the future I hope the keyword index will be a full +C text index, then i will activate more of the code ive +C just added for the keywords. Also I ought to list out the +C contents of the brief.idx entry for any matches found. +C 16-12-91 Added routines to deal with codata format and modified +C others accordingly. Externally only calls to cdroml are +C affected. At the moment am only dealing with an entryname +C accession number, and brief directory indexes for codata and, +C untidily offering more to the user +C which wil reslut in error messages (eg keyword searches). +C Have called codata format 'ltype b' +C 25-2-92 Added routines to deal with genbank format (as for codata) +C Added full text index search for embl and swissprot. This +C needs two integer arrays of size nrecen/32 which +C are passed down from main. Also removed filename strings from +C main. +C Have replaced the keyword search by the full text search. +C 26-2-92 Fixed bug: ftype is now cleared in rdlb1 +C 2-3-92 set filnam = ' ' for calls to openf1 +C 3-3-92 correction to keyword access - use (irec-1)*recordsize +C NOT irec*recordsize +C 11-3-92 Added more error messages, allowed file names of 80 characters +C 12-5-92 added author searches (replaced brief.idx search) +C 14-5-92 minimised the number of array elements used by the bit arrays +C 20-5-92 moved getrs, getrsl seekrs to seeme + SUBROUTINE RDSEQ(SEQ,MAXSEQ,IDIMT,J1,J2,ISTART,IEND, + +IDIM1,IDIMB,IDEV,FILNAM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH, + +IDEVOT,IFORNO,IDEVLL,IDEVEN,IDEVAN,IDEVDL, + +IDEVLF,LIBIN,LIBLF,WORKI,MAXWOR,IOK) + CHARACTER SEQ(MAXSEQ) + CHARACTER FILNAM*(*),LIBLF*(*) + CHARACTER HELPF*(*) + INTEGER ANSF,STYPE,WORKI(MAXWOR) + PARAMETER (MAXPRM = 16) + CHARACTER PROMPT(6)*(MAXPRM) + IDIMIN=IDIMT +1 CONTINUE + PROMPT(1) = 'Personal file' + PROMPT(2) = 'Sequence library' + STYPE = IFORNO + 1 + CALL RADION('Select sequence source',PROMPT,2,STYPE, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(STYPE.LT.1) GO TO 10 + IF(STYPE.EQ.1) THEN + PROMPT(1) = 'Staden' + PROMPT(2) = 'EMBL' + PROMPT(3) = 'GenBank' + PROMPT(4) = 'PIR' + PROMPT(5) = 'GCG' + PROMPT(6) = 'FASTA' + ANSF = 1 + CALL RADION('Select sequence file format',PROMPT,6,ANSF, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANSF.LT.1)GO TO 10 + FILNAM = ' ' + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + + 'Sequence file name', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)GO TO 10 + IDIMT=MAXSEQ + IF(ANSF.EQ.2)THEN +C READ FROM EMBL FILE + CALL REMBL(IDEV,SEQ(1),IDIMT,KBOUT) + ELSE IF(ANSF.EQ.5)THEN + CALL RGCG(IDEV,SEQ(1),IDIMT,KBOUT) + ELSE IF(ANSF.EQ.3)THEN +C READ FROM GENBANK FILE + CALL RGEN(IDEV,SEQ(1),IDIMT,KBOUT) + ELSE IF(ANSF.EQ.1)THEN +C READ FROM STADEN FILE + CALL ARRFIL(IDEV,SEQ,IDIMT,KBOUT) +C REMOVE CONTIG NAME IF PRESENT + IF(SEQ(20).EQ.'>')THEN + CALL MOVEC( + + SEQ(1),IDIMT,IDIMT,1,-20) + IDIMT=IDIMT-20 + WRITE(KBOUT,*)' Contig title removed' + END IF + ELSE IF(ANSF.EQ.4)THEN +C READ FROM A PIR FILE + IDIMT=MAXSEQ + CALL RDPIRP(SEQ(1),IDIMT, + + FILNAM,IDEV,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + ELSE IF(ANSF.EQ.6)THEN +C READ FROM A FASTA FILE + IDIMT=MAXSEQ + CALL RDFASP(SEQ(1),IDIMT, + + FILNAM,IDEV,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + END IF + ELSE IF(STYPE.EQ.2)THEN +C READ FROM A LIBRARY FILE + IDIMT=MAXSEQ + CALL RDLIB(SEQ,IDIMT,FILNAM,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IDEVOT,IDEVLL,IDEVEN,IDEVAN,IDEVDL, + + IDEVLF,LIBIN,LIBLF,WORKI,MAXWOR,IOK) + END IF + CLOSE (UNIT=IDEV) +10 CONTINUE + IF(IDIMT.EQ.0)IDIMT = IDIMIN + WRITE(KBOUT,1001)IDIMT +1001 FORMAT(' Sequence length ',I6) + J1=1 + J2=MIN(MAXSEQ,IDIMT) + ISTART=J1 + IEND=J2 + IDIMB=IEND-ISTART+1 + IDIM1=J2-J1+1 + END + SUBROUTINE REMBL(IDEV,SEQ,IDIM,KBOUT) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),CODE*2 +10 CONTINUE + READ(IDEV,1000,END=50)CODE +1000 FORMAT(A) +C sequence? + IF(CODE.NE.'SQ')GO TO 10 + IP1=1 + IP2=60 +20 CONTINUE + READ(IDEV,1002,END=30)CODE,(SEQ(K),K=IP1,IP2) +1002 FORMAT(A,3X,6(10A1,1X)) +C end of data? + IF(CODE(1:1).NE.'/')THEN +C sequence + IP1=IP1+60 + IP2=IP2+60 +C check for overflow + IF(IP2.GT.IDIM)IP2=IDIM + IF(IP1.LE.IP2)GO TO 20 + WRITE(KBOUT,1005)IDIM +1005 FORMAT(' Maximum sequence length',I6, + + ' exceeded, no more read') + RETURN + END IF +30 CONTINUE +C find end of data + IP3=IP2+1 +40 IP3=IP3-1 + IF(IP3.GT.0)THEN + IF(SEQ(IP3).EQ.' ')GO TO 40 + END IF +C end found + IDIM=IP3 + RETURN +50 CONTINUE +C NO DATA FOUND + IDIM=0 + END + SUBROUTINE RGEN(IDEV,SEQ,IDIM,KBOUT) +C AUTHOR: RODGER STADEN + CHARACTER LINE*80,LINE2*60,SEQ(IDIM) +C THIS ROUTINE READS A GENBANK FILE. +C IT ASSUMES THE WORD ORIGIN APPEARS ON THE LINE +C IMMEDIATELY PRECEDING THE SEQUENCE, AND THAT +C THE LAST LINE CONTAINS // + IDIMIN=IDIM + IDIM=0 +10 READ(IDEV,1000,END=40)LINE +1000 FORMAT(A) + IF(LINE(1:6).NE.'ORIGIN')GO TO 10 +20 READ(IDEV,1000,END=40)LINE + IF(LINE(1:2).NE.'//')THEN + LINE2(1:60)= + + LINE(11:20)//LINE(22:31)//LINE(33:42)//LINE(44:53)// + + LINE(55:64)//LINE(66:75) + DO 30 I=1,60 + IF(LINE2(I:I).EQ.' ')GO TO 40 + IF(IDIM.LT.IDIMIN)THEN + IDIM=IDIM+1 + SEQ(IDIM)=LINE2(I:I) + ELSE +C OVERFLOW! + WRITE(KBOUT,1001)IDIMIN +1001 FORMAT(' Maximum sequence length=',I6,'. Only this much read') + GO TO 40 + END IF +30 CONTINUE + GO TO 20 + END IF +40 CONTINUE +100 CONTINUE + CLOSE(UNIT=IDEV) + END + SUBROUTINE RDPIRP(SEQ,IDIM,FILNAM,IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C AUTHOR RODGER STADEN + CHARACTER FILNAM*(*) + CHARACTER SEQ(IDIM),TEMP(80),NAME*20,NAMIN*20,NEWNAM*20 + CHARACTER HELPF*(*) + EXTERNAL NOTRL,NMMTCH + IDIMIN = IDIM + IDIM = 0 + IOK = 1 + CALL YESNO(LIST,'Skip listing of entry names', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(LIST.LT.0) RETURN +4 CONTINUE + LENGTH = NOTRL(NAMIN,20,' ') + CALL GTSTR('Entry name',NAMIN,NEWNAM, + + LENGTH,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 4 + END IF + IF (LENGTH.GT.0) NAMIN = NEWNAM + CALL CCASE(NAMIN,1) +1005 FORMAT(A) +10 CONTINUE + READ(IDEV,1005,ERR=200,END=300)NAME + IF(NAME(1:1).NE.'>')GO TO 10 + IF(LIST.EQ.1)WRITE(KBOUT,1003)NAME +1003 FORMAT(' ',A) +C IS THIS THE ENTRY WE WANT? +C IF(NAME(5:).NE.NAMIN(1:12))GO TO 10 + IF(NMMTCH(NAME(5:),NAMIN).NE.0) GO TO 10 + FILNAM(1:16)=NAME(5:20) + CALL RPIR(SEQ,IDIMIN,IDEV,KBOUT,TEMP,IOK) + IDIM = IDIMIN + RETURN +200 CONTINUE + IOK = 1 + CALL ERROM(KBOUT,'Error reading file') + RETURN +300 CONTINUE + IOK = 1 + CALL ERROM(KBOUT,'Unexpected end of file') + END + SUBROUTINE RDPIRA(SEQ,IDIM, + + IDEV,KBOUT,TITLE,FILNAM,LIST,NAMIN,IDEVL) +C AUTHOR RODGER STADEN + CHARACTER TITLE*(*),FILNAM*(*) + PARAMETER (NAMLEN = 10) + CHARACTER SEQ(IDIM),TEMP(80),NAMIN*(*) + EXTERNAL NMMTCH + IF(LIST.EQ.1) THEN + READ(IDEVL,1005,ERR=999,END=950)NAMIN + END IF +10 CONTINUE + READ(IDEV,1005,ERR=200,END=300)TITLE +1005 FORMAT(A) + IF(TITLE(1:1).NE.'>')GO TO 10 + IF(LIST.EQ.1)THEN + IF(NMMTCH(TITLE(5:),NAMIN).NE.0) GO TO 10 + END IF + IF(LIST.EQ.-1)THEN + IF(NMMTCH(TITLE(5:),NAMIN).EQ.0) THEN + READ(IDEVL,1005,ERR=999,END=900)NAMIN + GO TO 10 + END IF + END IF + L = INDEX(TITLE(5:),' ') + IF(L.NE.0) THEN + L = 4 + L - 1 + ELSE + L = NAMLEN + END IF + FILNAM = TITLE(5:L) +C GOT WANTED SEQUENCE SO READ IT. FIRST READ 1 LINE TITLE + CALL RDPIRS(SEQ,IDIM,TEMP,TITLE,IDEV,KBOUT,IOK) + IF(IOK.EQ.1) GO TO 200 + IF(IOK.EQ.2) GO TO 300 + RETURN +200 CONTINUE + CALL ERROM(KBOUT,'Error reading library file') + IOK = 1 + IDIM = -9 + RETURN +300 CONTINUE + CALL ERROM(KBOUT,'End of library file reached') + IDIM = -9 + IOK = 2 + RETURN +900 CONTINUE + NAMIN = ' RODGER' + GO TO 10 +999 CONTINUE + CALL ERROM(KBOUT,'Error reading entry names file') + IDIM = -9 + RETURN + 950 CONTINUE + IOK = 3 + IDIM = -9 + END + SUBROUTINE RDPIRD(SEQ,IDIM, + + IDEV,KBOUT,TITLE,NAMIN) +C AUTHOR RODGER STADEN +C 20-12-90 Removed lines setting idim to 0. Could it have ever worked! + CHARACTER TITLE*(*) + CHARACTER SEQ(IDIM),TEMP(80),NAMIN*(*) + EXTERNAL NMMTCH +10 CONTINUE + READ(IDEV,1001,ERR=200,END=300)TITLE + IF(TITLE(1:1).NE.'>')GO TO 10 + IF(NMMTCH(TITLE(5:),NAMIN).NE.0) GO TO 10 +1001 FORMAT(A) + CALL RDPIRS(SEQ,IDIM,TEMP,TITLE,IDEV,KBOUT,IOK) + IF(IOK.EQ.1) GO TO 200 + IF(IOK.EQ.2) GO TO 300 + RETURN +200 CONTINUE + CALL ERROM(KBOUT,'Error reading library file') + IOK = 1 + IDIM = -9 + RETURN +300 CONTINUE + CALL ERROM(KBOUT,'End of library file reached') + IOK = 2 + IDIM = -9 + END + SUBROUTINE RDPIRS(SEQ,IDIM,TEMP,TITLE,IDEV,KBOUT,IOK) + CHARACTER SEQ(IDIM),TEMP(80),TITLE*(*) + IDIMIN = IDIM + IDIM = 0 + ISEQ = 0 + IOK = 0 + READ(IDEV,1005,ERR=200,END=300)TITLE +1005 FORMAT(A) + NCHRS = 80 +20 CONTINUE + READ(IDEV,1000,ERR=200,END=300)TEMP +1000 FORMAT(80A1) + DO 40 J=1,NCHRS + IF(TEMP(J).NE.' ')THEN +C IS THIS THE END OF THE ENTRY SHOWN BY A * ? + IF(TEMP(J).EQ.'*')THEN + IDIM = ISEQ + RETURN + END IF + ISEQ = ISEQ + 1 + IF(ISEQ.GT.IDIMIN)THEN + WRITE(KBOUT,1010)IDIMIN +1010 FORMAT(' Maximum sequence length (',I7,') reached', + + ' no more read') + IDIM = IDIMIN + RETURN + END IF + SEQ(ISEQ) = TEMP(J) + END IF +40 CONTINUE + GO TO 20 +200 CONTINUE + IOK = 1 + IDIM = -9 + RETURN +300 CONTINUE + IDIM = -9 + IOK = 2 + END + SUBROUTINE RPIR(SEQ,IDIM,IDEV,KBOUT,TEMP,IOK) + CHARACTER SEQ(IDIM),TEMP(80) + IDIMIN = IDIM + ISEQ = 0 + IOK = 0 +1000 FORMAT(80A1) + READ(IDEV,1000,ERR=200,END=200)TEMP + WRITE(KBOUT,1014)(TEMP(K),K=1,60) +1014 FORMAT(' ',60A) +20 CONTINUE + READ(IDEV,1000,ERR=200,END=300)TEMP + DO 40 J=1,80 + IF(TEMP(J).NE.' ')THEN + IF(TEMP(J).EQ.'*')GO TO 100 + ISEQ=ISEQ+1 + IF(ISEQ.GT.IDIMIN)THEN + WRITE(KBOUT,1010)IDIMIN +1010 FORMAT(' Maximum sequence length (',I6,') reached.', + + ' No more read') + GO TO 400 + END IF + SEQ(ISEQ)=TEMP(J) + END IF +40 CONTINUE + GO TO 20 +100 CONTINUE + IDIM=ISEQ + RETURN +200 CONTINUE + IOK = 1 + CALL ERROM(KBOUT,'Error reading file') + RETURN +300 CONTINUE + WRITE(KBOUT,*)' Warning: No * at end of entry' + IDIM = ISEQ + RETURN +400 CONTINUE + IDIM=IDIMIN + END + SUBROUTINE RDFASP(SEQ,IDIM,FILNAM,IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C AUTHOR RODGER STADEN + CHARACTER FILNAM*(*) + CHARACTER SEQ(IDIM),TEMP(80),NAME*80,NAMIN*20,NEWNAM*20 + CHARACTER HELPF*(*) + EXTERNAL NOTRL,NMMTCH + SAVE NAMIN + DATA NAMIN/' '/ + IF (NAMIN(1:1).EQ.' ')CALL GFASNM(IDEV,NAME,NAMIN) + IDIMIN = IDIM + IDIM = 0 + IOK = 1 + CALL YESNO(LIST,'Skip listing of entry names', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(LIST.LT.0) RETURN +4 CONTINUE + LENGTH = NOTRL(NAMIN,20,' ') + CALL GTSTR('Entry name',NAMIN,NEWNAM, + + LENGTH,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 4 + END IF + IF (LENGTH.GT.0) NAMIN = NEWNAM + CALL CCASE(NAMIN,1) +1005 FORMAT(A) +10 CONTINUE + READ(IDEV,1005,ERR=200,END=300)NAME + IF(NAME(1:1).NE.'>')GO TO 10 + IF(LIST.EQ.1)WRITE(KBOUT,1003)NAME +1003 FORMAT(' ',A) +C IS THIS THE ENTRY WE WANT? +C IF(NAME(5:).NE.NAMIN(1:12))GO TO 10 + IF(NMMTCH(NAME(2:),NAMIN).NE.0) GO TO 10 + FILNAM = NAME(2:INDEX(NAME,' ')) + CALL RFAS(SEQ,IDIMIN,IDEV,KBOUT,TEMP,IOK) + IDIM = IDIMIN + RETURN +200 CONTINUE + IOK = 1 + CALL ERROM(KBOUT,'Error reading file') + RETURN +300 CONTINUE + IOK = 1 + CALL ERROM(KBOUT,'Unexpected end of file') + END + SUBROUTINE GFASNM(IDEV,LINE,NAMIN) + CHARACTER LINE*(*),NAMIN*(*) + EXTERNAL NOTRL + 10 CONTINUE + READ(IDEV,1000,END=30,ERR=30)LINE + IF (LINE(1:1).NE.'>') GO TO 10 + I = MIN(INDEX(LINE,' ')-1,LEN(NAMIN)) + NAMIN = LINE(2:I) + 30 CONTINUE + REWIND(UNIT=IDEV) + 1000 FORMAT(A) + END + SUBROUTINE RDFASA(SEQ,IDIM, + + IDEV,KBOUT,TITLE,FILNAM,LIST,NAMIN,IDEVL,ENTRYN) +C AUTHOR RODGER STADEN + CHARACTER TITLE*(*),FILNAM*(*) + PARAMETER (NAMLEN = 10) + CHARACTER SEQ(IDIM),TEMP*80,NAMIN*(*) + INTEGER ENTRYN + EXTERNAL NMMTCH + SAVE TEMP +C +C for first entry we must find the entry name, but on subsequent visits +C we assume we have an entryname in title. This is because we have no +C terminator and have to keep reading until we meet > +C + IF(LIST.EQ.1) THEN + READ(IDEVL,1005,ERR=999,END=950)NAMIN + END IF + IF (ENTRYN.EQ.0) THEN + 5 CONTINUE + READ(IDEV,1005,ERR=200,END=300)TITLE +1005 FORMAT(A) + IF(TITLE(1:1).NE.'>')GO TO 5 + ELSE + TITLE = TEMP + END IF + ENTRYN = 1 +10 CONTINUE +C write(*,*)namin +C write(*,*)title + IF(LIST.EQ.1)THEN + IF(NMMTCH(TITLE(2:),NAMIN).NE.0) THEN +20 CONTINUE + READ(IDEV,1005,ERR=200,END=300)TITLE + IF(TITLE(1:1).NE.'>')GO TO 20 + IF(NMMTCH(TITLE(2:),NAMIN).NE.0) GO TO 20 + END IF + END IF + IF(LIST.EQ.-1)THEN + IF(NMMTCH(TITLE(2:),NAMIN).EQ.0) THEN +30 CONTINUE + READ(IDEVL,1005,ERR=999,END=900)NAMIN +C write(*,*)'newnam',namin +40 CONTINUE + READ(IDEV,1005,ERR=200,END=300)TITLE + IF(TITLE(1:1).NE.'>')GO TO 40 +C write(*,*)'newtit',title + IF(NMMTCH(TITLE(2:),NAMIN).EQ.0) GO TO 30 + END IF + END IF + L = INDEX(TITLE,' ') + IF(L.NE.0) THEN + L = L - 1 + ELSE + L = NAMLEN + END IF + FILNAM = TITLE(2:L) +C GOT WANTED SEQUENCE SO READ IT. FIRST READ 1 LINE TITLE + CALL RDFASS(SEQ,IDIM,TEMP,TITLE,IDEV,KBOUT,IOK) + IF(IOK.EQ.1) GO TO 200 + IF(IOK.EQ.2) GO TO 300 +C write(*,*)'process',filnam + RETURN +200 CONTINUE + CALL ERROM(KBOUT,'Error reading library file') + IOK = 1 + IDIM = -9 + RETURN +300 CONTINUE + CALL ERROM(KBOUT,'End of library file reached') + IDIM = -9 + IOK = 2 + RETURN +900 CONTINUE +C +C end of names reached for list of excludes so set silly name +C and read to next entry (allows last entry to be excluded) +C +50 CONTINUE + READ(IDEV,1005,ERR=200,END=300)TITLE + IF(TITLE(1:1).NE.'>')GO TO 50 + NAMIN = ' RODGER' + GO TO 10 +999 CONTINUE + CALL ERROM(KBOUT,'Error reading entry names file') + IDIM = -9 + RETURN + 950 CONTINUE + IOK = 3 + IDIM = -9 + END + SUBROUTINE RDFASD(SEQ,IDIM, + + IDEV,KBOUT,TITLE,NAMIN) +C AUTHOR RODGER STADEN + CHARACTER TITLE*(*) + CHARACTER SEQ(IDIM),TEMP*80,NAMIN*(*) + EXTERNAL NMMTCH +10 CONTINUE + READ(IDEV,1001,ERR=200,END=300)TITLE + IF(TITLE(1:1).NE.'>')GO TO 10 + IF(NMMTCH(TITLE(2:),NAMIN).NE.0) GO TO 10 +1001 FORMAT(A) + CALL RDFASS(SEQ,IDIM,TEMP,TITLE,IDEV,KBOUT,IOK) + IF(IOK.EQ.1) GO TO 200 + IF(IOK.EQ.2) GO TO 300 + RETURN +200 CONTINUE + CALL ERROM(KBOUT,'Error reading library file') + IOK = 1 + IDIM = -9 + RETURN +300 CONTINUE + CALL ERROM(KBOUT,'End of library file reached') + IOK = 2 + IDIM = -9 + END + SUBROUTINE RDFASS(SEQ,IDIM,TEMP,TITLE,IDEV,KBOUT,IOK) + CHARACTER SEQ(IDIM),TEMP*(*),TITLE*(*) + IDIMIN = IDIM + IDIM = 0 + ISEQ = 0 + IOK = 0 + NCHRS = 80 +20 CONTINUE + READ(IDEV,1000,ERR=200,END=300)TEMP +1000 FORMAT(A) +C write(*,1000)temp + IF(TEMP(1:1).EQ.'>') THEN + IDIM = ISEQ +C write(*,*)idim + RETURN + END IF + DO 40 J=1,NCHRS + IF(TEMP(J:J).NE.' ')THEN + ISEQ = ISEQ + 1 + IF(ISEQ.GT.IDIMIN)THEN + WRITE(KBOUT,1010)IDIMIN +1010 FORMAT(' Maximum sequence length (',I7,') reached', + + ' no more read') + IDIM = IDIMIN + RETURN + END IF + SEQ(ISEQ) = TEMP(J:J) + END IF +40 CONTINUE + GO TO 20 +200 CONTINUE + IOK = 1 + IDIM = -9 + RETURN +300 CONTINUE + IDIM = ISEQ + END + SUBROUTINE RFAS(SEQ,IDIM,IDEV,KBOUT,TEMP,IOK) + CHARACTER SEQ(IDIM),TEMP(80) + IDIMIN = IDIM + ISEQ = 0 + IOK = 0 +1000 FORMAT(80A1) +20 CONTINUE + READ(IDEV,1000,ERR=200,END=100)TEMP + IF(TEMP(1).EQ.'>')GO TO 100 + DO 40 J=1,80 + IF(TEMP(J).NE.' ')THEN + ISEQ=ISEQ+1 + IF(ISEQ.GT.IDIMIN)THEN + WRITE(KBOUT,1010)IDIMIN +1010 FORMAT(' Maximum sequence length (',I6,') reached.', + + ' No more read') + GO TO 400 + END IF + SEQ(ISEQ)=TEMP(J) + END IF +40 CONTINUE + GO TO 20 +100 CONTINUE + IDIM=ISEQ + RETURN +200 CONTINUE + IOK = 1 + CALL ERROM(KBOUT,'Error reading file') + RETURN +400 CONTINUE + IDIM=IDIMIN + END + SUBROUTINE RGCG(IDEV,SEQ,MAXSEQ,KBOUT) + CHARACTER LINE*133,SEQ(MAXSEQ) + INTEGER GCGDOT + EXTERNAL GCGDOT + IOK = GCGDOT(IDEV,LINE) + IF(IOK.NE.0) THEN + WRITE(KBOUT,*)'No .. line found' + MAXSEQ = 0 + CLOSE(UNIT=IDEV) + RETURN + END IF + CALL RGCGIN(SEQ,MAXSEQ,LINE,IDEV,KBOUT) + CLOSE(UNIT=IDEV) + END + INTEGER FUNCTION GCGDOT(IDEV,LINE) + CHARACTER LINE*(*) + GCGDOT = 1 +10 CONTINUE + READ(IDEV,1000,ERR=100,END=100)LINE +1000 FORMAT(A) + I = INDEX(LINE,'..') + IF(I.EQ.0) GO TO 10 + GCGDOT = 0 + RETURN +100 CONTINUE + END + SUBROUTINE RGCGIN(SEQ,MAXSEQ,LINE,IDEV,KBOUT) + CHARACTER SEQ(MAXSEQ) + PARAMETER (NBAD=11) + CHARACTER LINE*(*),CHARB*(NBAD) + PARAMETER (CHARB='0123456789 ') + INTEGER COKBAD + EXTERNAL COKBAD + ISEQ = 0 +10 CONTINUE + READ(IDEV,1000,ERR=100,END=200)LINE +1000 FORMAT(A) + DO 5 I = 1,LEN(LINE) + IF(COKBAD(LINE(I:I),CHARB,NBAD).EQ.0) THEN + ISEQ = ISEQ + 1 + IF(ISEQ.GT.MAXSEQ) THEN + WRITE(KBOUT,1001)MAXSEQ +1001 FORMAT(' Maximum sequence length',I6,' exceeded') + RETURN + END IF + SEQ(ISEQ) = LINE(I:I) + END IF +5 CONTINUE + GO TO 10 +100 CONTINUE + CALL ERROM(KBOUT,'Error reading file') +200 CONTINUE + MAXSEQ = ISEQ + END + INTEGER FUNCTION COKBAD(CHAR,BADC,NBAD) + CHARACTER CHAR,BADC*(*) + COKBAD = 1 + DO 10 I = 1,NBAD + IF(CHAR.EQ.BADC(I:I)) RETURN +10 CONTINUE + COKBAD = 0 + END +C routines for handling embl cdrom format files +C +C RDLIB opens cdrom format library and selects options +C CDROMS performs a number of jobs on a cdrom format library: +C get a sequence, get annotations, get entryname from accession no +C OCDLBS gets the file names and types of a cdrom format lib for use by CDROMS +C CDROML performs a number of jobs on a cdrom format library relating +C to its use when library searching: read the next entry off the +C entryname file, and get the seq; get the next named seq; get +C the next seq not on a list of excluded seqs; get a named seq +C OCDLBL opens a library for use by CDROML +C RDLB0 reads a list of libraries, gets the names of their descriptor files +C and the prompt to appear on the screen +C RDLB1 gets a list of the file names and file types for a particular lib + SUBROUTINE RDLB0(LTYPE,LOGNAM,PROMPT,MAXMEN,NAMLEN,MAXPRM, + +IDEVLS,FILNLL,LINE,MAXLIN,KBOUT,ITEM,IOK) + CHARACTER PROMPT(MAXMEN)*(*),LOGNAM(MAXMEN)*(*),LTYPE*(*) + CHARACTER LINE*(*),FILNLL*(*) +C Read file until the desired line is found + CALL OPENRS(IDEVLS,FILNLL,IOK,LRECL,2) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Unable to open file of library names') + CALL ERROM(KBOUT,FILNLL) + RETURN + END IF + LINENO = 0 + ITEM = 1 +C Read and parse until end of data + CALL RDLB2(LTYPE,LOGNAM,PROMPT,MAXMEN,NAMLEN,MAXPRM,IDEVLS, + +LINE,MAXLIN,LINENO,ITEM,IOK) + IF(IOK.NE.0) + +WRITE(KBOUT,*)'Error in library menu file on line',LINENO + ITEM = ITEM - 1 + CLOSE(UNIT=IDEVLS) + END + SUBROUTINE RDLB2(LTYPE,LOGNAM,PROMPT,MAXMEN,NAMLEN,MAXPRM, + +IDEVM,LINE,MAXLIN,LINENO,ITEM,IOK) + CHARACTER PROMPT(MAXMEN)*(*),LOGNAM(MAXMEN)*(*),LTYPE*(*) + CHARACTER LINE*(*) + IOK = 0 +C Read and parse until end of data +C +10 CONTINUE + LINENO = LINENO + 1 + READ(IDEVM,1000,ERR=100,END=200)LINE +1000 FORMAT(A) + CALL RDLB3(LTYPE(ITEM:ITEM),LINE,MAXLIN,LOGNAM(ITEM), + + PROMPT(ITEM), + + NAMLEN,MAXPRM,IOK) + IF(IOK.EQ.0) THEN + ITEM = ITEM + 1 + END IF + GO TO 10 +100 CONTINUE + IOK = 1 + CALL ERROM(KBOUT,'Error reading list of library files') + RETURN +200 CONTINUE + IOK = 0 + END + SUBROUTINE RDLB3(LTYPE,LINE,MAXLIN,LOGNAM,PROMPT,NAMLEN, + +MAXPRM,IOK) + CHARACTER LTYPE,LINE*(*),LOGNAM*(*),PROMPT*(*),COMMNT + PARAMETER (COMMNT = '!') + EXTERNAL NOTILR,NOTIRL +C Extract LOGNAM, PROMPT + IOK = 1 + MAXLN = INDEX(LINE,COMMNT) - 1 + IF(MAXLN.EQ.-1) MAXLN = MAXLIN + IF(MAXLN.LT.6) RETURN + LOGS = NOTILR(LINE(2:),MAXLN-1,' ') + 1 + IF(LOGS.EQ.MAXLN) RETURN + LOGE = INDEX(LINE(LOGS:),' ') + IF(LOGE.EQ.0) RETURN + LOGE = LOGE - 1 + LOGEE = LOGE + LOGE = MIN(NAMLEN,LOGE) + LOGE = LOGE + LOGS - 1 + LOGNAM = ' ' + LOGNAM = LINE(LOGS:LOGE) + LOGEE = LOGEE + LOGS - 1 + LOGS = NOTILR(LINE(LOGEE+1:),MAXLN,' ') + LOGEE + IF(LOGS.EQ.MAXLN) RETURN + LOGE = NOTIRL(LINE,MAXLN,' ') + IF(LOGE.EQ.0) RETURN + LOGE = MIN(MAXPRM,LOGE-LOGS+1) + LOGS - 1 + PROMPT = ' ' + PROMPT = LINE(LOGS:LOGE) + LTYPE = LINE(1:1) + IOK = 0 + END + SUBROUTINE RDLB1(LIBNAM,MAXMEN,NAMLEN,IDEVLS, + +FILNAM, + +LINE,MAXLIN,FTYPE,LINENO,KBOUT,ITEM,IOK) + CHARACTER LIBNAM(MAXMEN)*(*) + CHARACTER LINE*(*),FTYPE*(*),FILNAM*(*) + IOK = 0 +C ftype(i:i) stores the file type for libnam(i) +C and libnam(i) stores the name of a library file +C Read file until the desired line is found + FTYPE = ' ' + CALL OPENRS(IDEVLS,FILNAM,IOK,LRECL,2) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Unable to open file of library file names') + CALL ERROM(KBOUT,FILNAM) + RETURN + END IF + LINENO = 0 + ITEM = 1 +C Read and parse until end of data +C +10 CONTINUE + LINENO = LINENO + 1 + READ(IDEVLS,1000,ERR=100,END=200)LINE +1000 FORMAT(A) + CALL RDLB4(LINE,MAXLIN,FTYPE(ITEM:ITEM),LIBNAM(ITEM), + + NAMLEN,IOK) + IF(IOK.EQ.0) THEN + ITEM = ITEM + 1 + END IF + GO TO 10 +100 CONTINUE + IOK = 1 + CALL ERROM(KBOUT,'Error reading names of library files') + RETURN +200 CONTINUE + ITEM = ITEM - 1 + IOK = 0 + END + SUBROUTINE RDLB4(LINE,MAXLIN,LTYPE,LIBNAM,NAMLEN,IOK) + CHARACTER LINE*(*),LTYPE,LIBNAM*(*),COMMNT + PARAMETER (COMMNT = '!') + EXTERNAL NOTILR +C Extract LTYPE, LIBNAM + IOK = 1 + MAXLN = INDEX(LINE,COMMNT) - 1 + IF(MAXLN.EQ.-1) MAXLN = MAXLIN + IF(MAXLN.LT.6) RETURN + LOGS = NOTILR(LINE(2:),MAXLN-1,' ') + 1 + IF(LOGS.EQ.MAXLN) RETURN + LOGE = INDEX(LINE(LOGS:),' ') + IF(LOGE.EQ.0) RETURN + LOGE = LOGE - 1 + LOGEE = LOGE + LOGE = MIN(NAMLEN,LOGE) + LOGE = LOGE + LOGS - 1 + LIBNAM = ' ' + LIBNAM = LINE(LOGS:LOGE) + LTYPE = LINE(1:1) + IOK = 0 + END + SUBROUTINE RDLIB(SEQ,IDIM,FILNAM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IDEVOT,IDEVLL,IDEVEN,IDEVAN,IDEVDL, + +IDEVLF,LIBIN,LIBLF,WORKI,MAXWOR,IOK) +C AUTHOR RODGER STADEN +C IDEVLL = unit for library list LIBLF +C IDEVOT = unit for output of results +C IDEVEN = entry name file ENAMEF +C IDEVAN = unit for accession number files ANUMTF ANUMHF and keywords +C IDEVDL = division lookup file DIVLUF +C IDEVLF = actual library data files DATAF +C note to me: there is really no need to pass all these character strings +C down from main: you have to declare the size here anyway. So sort it out! + PARAMETER (MAXNAM = 80,LENNAM = 10, MAXKWD = 5) + PARAMETER (MAXPRM = 50,MAXMEN=20,MAXLIN=80,MAXLBF=9) + CHARACTER*(MAXNAM) ENAMEF,DIVLUF,DATAF,ANUMTF,ANUMHF + CHARACTER*(MAXNAM) AUTHHF,AUTHTF + CHARACTER FILNAM*(*),LIBLF*(*) + CHARACTER SEQ(IDIM),NAMIN*10,NEWNAM*10 + CHARACTER HELPF*(*),ACNUM*10 + CHARACTER PROMPT(MAXMEN)*(MAXPRM),LOGNAM(MAXMEN)*(MAXNAM) + CHARACTER FTYPE*(MAXLBF),LINE*(MAXLIN),LIBNAM(MAXLBF)*(MAXNAM) + CHARACTER LTYPE*(MAXMEN) + CHARACTER*(MAXNAM) BRIEFF,KWRDTF,KWRDHF + CHARACTER KEYWDS*80,TITLE*80 + INTEGER WORKI(MAXWOR) + INTEGER KWS(MAXKWD),KWE(MAXKWD) + INTEGER DIVCOD,ANNOFF,SEQOFF + EXTERNAL NOTRL,NMMTCH + IDIMIN = IDIM + IDIM = 0 + NAMIN = ' ' + ACNUM = ' ' + NAMIN = ' ' + LIB = LIBIN +1 CONTINUE + CALL RDLB0(LTYPE,LOGNAM,PROMPT,MAXMEN,MAXNAM,MAXPRM, + +IDEVLL,LIBLF,LINE,MAXLIN,KBOUT,ITEM,IOK) + IF(IOK.NE.0) RETURN + IOK = 1 + CALL RADION('Select a library',PROMPT,ITEM,LIB, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(LIB.LT.1) RETURN + FILNAM = LOGNAM(LIB) + CALL RDLB1(LIBNAM,MAXLBF,MAXNAM,IDEVLL,FILNAM, + +LINE,MAXLIN,FTYPE,LINENO,KBOUT,ITEM,IOK) + IF(IOK.NE.0) RETURN + IF(LTYPE(LIB:LIB).EQ.'A') THEN + WRITE(KBOUT,*)'Library is in EMBL format with indexes' + CALL OCDLBS(LIBNAM,MAXNAM,ITEM,FTYPE, + + ENAMEF,DIVLUF,ANUMTF,ANUMHF,BRIEFF,KWRDTF,KWRDHF, + + AUTHHF,AUTHTF,IOK) + ELSE IF(LTYPE(LIB:LIB).EQ.'B') THEN + WRITE(KBOUT,*)'Library is in CODATA format with indexes' + CALL OCDLBS(LIBNAM,MAXNAM,ITEM,FTYPE, + + ENAMEF,DIVLUF,ANUMTF,ANUMHF,BRIEFF,KWRDTF,KWRDHF, + + AUTHHF,AUTHTF,IOK) + ELSE IF(LTYPE(LIB:LIB).EQ.'C') THEN + WRITE(KBOUT,*)'Library is in GenBank format with indexes' + CALL OCDLBS(LIBNAM,MAXNAM,ITEM,FTYPE, + + ENAMEF,DIVLUF,ANUMTF,ANUMHF,BRIEFF,KWRDTF,KWRDHF, + + AUTHHF,AUTHTF,IOK) + ELSE + WRITE(KBOUT,*)'Unknown library type' + RETURN + END IF +2 CONTINUE + IOPT = 1 + PROMPT(1) = 'Get a sequence' + PROMPT(2) = 'Get annotations' + PROMPT(3) = 'Get entry names from accession numbers' + PROMPT(4) = 'Search author index' + PROMPT(5) = 'Search text index for keywords' + CALL RADION('Select a task',PROMPT,5,IOPT, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOPT.LT.1) GO TO 1 +3 CONTINUE + IF((IOPT.EQ.1).OR.(IOPT.EQ.2)) THEN +C get seq or annot + LENGTH = NOTRL(NAMIN,LENNAM,' ') + CALL GTSTR('Entry name',NAMIN,NEWNAM, + + LENGTH,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) GO TO 2 + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 3 + END IF + IF(LENGTH.GT.0) NAMIN = NEWNAM + CALL CCASE(NAMIN,1) + CALL CDROMS(IOPT,NAMIN,ACNUM, + + ENAMEF,DIVLUF,ANUMTF,ANUMHF,DATAF,BRIEFF,KWRDTF,KWRDHF, + + IDEVEN,IDEVAN,IDEVDL,IDEVLF,KBIN,AUTHHF,AUTHTF, + + KBOUT,DIVCOD,SEQOFF,ANNOFF,SEQ,IDIMIN,IDEVOT, + + KEYWDS,KWS,KWE,MAXKEY,LTYPE(LIB:LIB),WORKI,MAXWOR,TITLE,IOK) + IF((IOPT.EQ.1).AND.(IOK.EQ.0)) IDIM = IDIMIN + FILNAM = NAMIN + CLOSE(UNIT=IDEVEN) + CLOSE(UNIT=IDEVAN) + CLOSE(UNIT=IDEVLF) + CLOSE(UNIT=IDEVDL) + ELSE IF(IOPT.EQ.3) THEN + 4 CONTINUE + LENGTH = NOTRL(ACNUM,LENNAM,' ') + CALL GTSTR('Accession number',ACNUM,NEWNAM, + + LENGTH,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.2) GO TO 2 + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 3 + END IF + IF(LENGTH.GT.0) ACNUM = NEWNAM + CALL CCASE(ACNUM,1) + CALL CDROMS(IOPT,NAMIN,ACNUM, + + ENAMEF,DIVLUF,ANUMTF,ANUMHF,DATAF,BRIEFF,KWRDTF,KWRDHF, + + IDEVEN,IDEVAN,IDEVDL,IDEVLF,KBIN,AUTHHF,AUTHTF, + + KBOUT,DIVCOD,SEQOFF,ANNOFF,SEQ,IDIMIN,IDEVOT, + + KEYWDS,KWS,KWE,MAXKEY,LTYPE(LIB:LIB),WORKI,MAXWOR,TITLE,IOK) + CLOSE(UNIT=IDEVEN) + CLOSE(UNIT=IDEVAN) + CLOSE(UNIT=IDEVLF) + CLOSE(UNIT=IDEVDL) + ELSE IF(IOPT.EQ.4) THEN + NKEYS = MAXKWD + CALL SRCKEZ(KBIN,KBOUT,KEYWDS,KWS,KWE,NKEYS,'Authors',IOK) + IF(IOK.NE.0) GO TO 2 + IF(NKEYS.LT.1) GO TO 2 + CALL CDROMS(IOPT,NAMIN,ACNUM, + + ENAMEF,DIVLUF,ANUMTF,ANUMHF,DATAF,BRIEFF,KWRDTF,KWRDHF, + + IDEVEN,IDEVAN,IDEVDL,IDEVLF,KBIN,AUTHHF,AUTHTF, + + KBOUT,DIVCOD,SEQOFF,ANNOFF,SEQ,IDIMIN,IDEVOT, + + KEYWDS,KWS,KWE,NKEYS,LTYPE(LIB:LIB),WORKI,MAXWOR,TITLE,IOK) + CLOSE(UNIT=IDEVEN) + GO TO 2 + ELSE IF(IOPT.EQ.5) THEN + NKEYS = MAXKWD + CALL SRCKEZ(KBIN,KBOUT,KEYWDS,KWS,KWE,NKEYS,'Keywords',IOK) + IF(IOK.NE.0) GO TO 2 + IF(NKEYS.LT.1) GO TO 2 + CALL CDROMS(IOPT,NAMIN,ACNUM, + + ENAMEF,DIVLUF,ANUMTF,ANUMHF,DATAF,BRIEFF,KWRDTF,KWRDHF, + + IDEVEN,IDEVAN,IDEVDL,IDEVLF,KBIN,AUTHHF,AUTHTF, + + KBOUT,DIVCOD,SEQOFF,ANNOFF,SEQ,IDIMIN,IDEVOT, + + KEYWDS,KWS,KWE,NKEYS,LTYPE(LIB:LIB),WORKI,MAXWOR,TITLE,IOK) + CLOSE(UNIT=IDEVEN) + GO TO 2 + END IF + IF(IOPT.EQ.1) RETURN + GO TO 2 + END + SUBROUTINE RDLIBB(SEQ,IDIM,FILNAM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IDEVOT,IDEVLL,IDEVEN,IDEVAN,IDEVDL, + +IDEVLF,LIBIN,LIBLF,WORKI,MAXWOR,IOK) +C AUTHOR RODGER STADEN +C routine for lip +C IDEVLL = unit for library list LIBLF +C IDEVOT = unit for output of results +C IDEVEN = entry name file ENAMEF +C IDEVAN = unit for accession number files ANUMTF ANUMHF and keywords +C IDEVDL = division lookup file DIVLUF +C IDEVLF = actual library data files DATAF +C note to me: there is really no need to pass all these character strings +C down from main: you have to declare the size here anyway. So sort it out! + PARAMETER (MAXNAM = 80,LENNAM = 10, MAXKWD = 5) + PARAMETER (MAXPRM = 50,MAXMEN=20,MAXLIN=80,MAXLBF=9) + CHARACTER*(MAXNAM) ENAMEF,DIVLUF,DATAF,ANUMTF,ANUMHF + CHARACTER*(MAXNAM) AUTHHF,AUTHTF + CHARACTER FILNAM*(*),LIBLF*(*) + CHARACTER SEQ(IDIM),NAMIN*10,NEWNAM*14 + CHARACTER HELPF*(*),ACNUM*10 + CHARACTER PROMPT(MAXMEN)*(MAXPRM),LOGNAM(MAXMEN)*(MAXNAM) + CHARACTER FTYPE*(MAXLBF),LINE*(MAXLIN),LIBNAM(MAXLBF)*(MAXNAM) + CHARACTER LTYPE*(MAXMEN) + CHARACTER*(MAXNAM) BRIEFF,KWRDTF,KWRDHF + CHARACTER KEYWDS*80,TITLE*80,EXT*4 + INTEGER WORKI(MAXWOR) + INTEGER KWS(MAXKWD),KWE(MAXKWD) + INTEGER DIVCOD,ANNOFF,SEQOFF,GNFFOF + EXTERNAL NOTRL,NMMTCH,GNFFOF + NAMIN = ' ' + ACNUM = ' ' + NAMIN = ' ' + LIB = LIBIN +1 CONTINUE + CALL RDLB0(LTYPE,LOGNAM,PROMPT,MAXMEN,MAXNAM,MAXPRM, + +IDEVLL,LIBLF,LINE,MAXLIN,KBOUT,ITEM,IOK) + IF(IOK.NE.0) RETURN + IOK = 1 + CALL RADION('Select a library',PROMPT,ITEM,LIB, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(LIB.LT.1) RETURN + FILNAM = LOGNAM(LIB) + CALL RDLB1(LIBNAM,MAXLBF,MAXNAM,IDEVLL,FILNAM, + +LINE,MAXLIN,FTYPE,LINENO,KBOUT,ITEM,IOK) + IF(IOK.NE.0) RETURN + IF(LTYPE(LIB:LIB).EQ.'A') THEN + WRITE(KBOUT,*)'Library is in EMBL format with indexes' + CALL OCDLBS(LIBNAM,MAXNAM,ITEM,FTYPE, + + ENAMEF,DIVLUF,ANUMTF,ANUMHF,BRIEFF,KWRDTF,KWRDHF, + + AUTHHF,AUTHTF,IOK) + ELSE IF(LTYPE(LIB:LIB).EQ.'B') THEN + WRITE(KBOUT,*)'Library is in CODATA format with indexes' + CALL OCDLBS(LIBNAM,MAXNAM,ITEM,FTYPE, + + ENAMEF,DIVLUF,ANUMTF,ANUMHF,BRIEFF,KWRDTF,KWRDHF, + + AUTHHF,AUTHTF,IOK) + ELSE IF(LTYPE(LIB:LIB).EQ.'C') THEN + WRITE(KBOUT,*)'Library is in GenBank format with indexes' + CALL OCDLBS(LIBNAM,MAXNAM,ITEM,FTYPE, + + ENAMEF,DIVLUF,ANUMTF,ANUMHF,BRIEFF,KWRDTF,KWRDHF, + + AUTHHF,AUTHTF,IOK) + ELSE + WRITE(KBOUT,*)'Unknown library type' + RETURN + END IF +2 CONTINUE + PROMPT(1) = 'Sequence only in FASTA format' + PROMPT(2) = 'Sequence only in STADEN format' + PROMPT(3) = 'Annotation only' + PROMPT(4) = 'Complete entries' + JOPT = 1 + CALL RADION('Select a format',PROMPT,4,JOPT, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(JOPT.LT.1) RETURN +C IOPT = 2 + FILNAM = ' ' + CALL OPENF1(IDEVLL,FILNAM,0,IOK,KBIN,KBOUT, + + 'File of entry names', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + IF (JOPT.EQ.1) THEN + EXT = '.seq' + IOPT = 1 + ELSE IF (JOPT.EQ.2) THEN + EXT = '.SEQ' + IOPT = 1 + ELSE IF (JOPT.EQ.3) THEN + EXT = '.txt' + IOPT = 2 + ELSE IF (JOPT.EQ.4) THEN + EXT = '.all' + IOPT = 6 + END IF +3 CONTINUE + IOK = GNFFOF(IDEVLL,NAMIN) + IF (IOK.EQ.1) GO TO 1 + IF (IOK.NE.0) GO TO 3 + NEWNAM = NAMIN + K = INDEX(NEWNAM,' ') + NEWNAM(K:K+3) = EXT + CALL OPENRS(IDEVOT,NEWNAM,IOK,LRECL,1) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Unable to open file') + CALL ERROM(KBOUT,NEWNAM) + ELSE + CALL CCASE(NAMIN,1) + IDIMIN = IDIM + CALL CDROMS(IOPT,NAMIN,ACNUM, + + ENAMEF,DIVLUF,ANUMTF,ANUMHF,DATAF,BRIEFF,KWRDTF,KWRDHF, + + IDEVEN,IDEVAN,IDEVDL,IDEVLF,KBIN,AUTHHF,AUTHTF, + + KBOUT,DIVCOD,SEQOFF,ANNOFF,SEQ,IDIMIN,IDEVOT, + + KEYWDS,KWS,KWE,MAXKEY,LTYPE(LIB:LIB),WORKI,MAXWOR,TITLE,IOK) + CLOSE(UNIT=IDEVEN) + CLOSE(UNIT=IDEVAN) + CLOSE(UNIT=IDEVLF) + CLOSE(UNIT=IDEVDL) + IF (JOPT.EQ.1) THEN + CALL WRITFF(IDEVOT,SEQ,IDIMIN,NAMIN,TITLE) + ELSE IF (JOPT.EQ.2) THEN + WRITE(IDEVOT,1001)NAMIN + WRITE(IDEVOT,1001)TITLE + 1001 FORMAT(';',A) + CALL FMTDKN(IDEVOT,SEQ,IDIMIN) + END IF + CLOSE(UNIT=IDEVOT) + END IF + GO TO 3 + END + SUBROUTINE OCDLBS(LIBNAM,NAMLEN,ITEMS,FTYPE, + +ENAMEF,DIVLUF,ANUMTF,ANUMHF,BRIEFF,KWRDTF,KWRDHF, + +AUTHHF,AUTHTF,IOK) +C Assigns libnam names to file name strings + CHARACTER LIBNAM(ITEMS)*(*),AUTHHF*(*),AUTHTF*(*) + CHARACTER ENAMEF*(*),DIVLUF*(*),BRIEFF*(*),KWRDTF*(*) + CHARACTER ANUMTF*(*),ANUMHF*(*),FTYPE*(*),KWRDHF*(*) + ENAMEF = ' ' + DIVLUF = ' ' + ANUMTF = ' ' + ANUMHF = ' ' + BRIEFF = ' ' + KWRDTF = ' ' + KWRDHF = ' ' + AUTHTF = ' ' + AUTHHF = ' ' + I = INDEX(FTYPE,'A') + IF(I.NE.0) DIVLUF = LIBNAM(I) + I = INDEX(FTYPE,'B') + IF(I.NE.0) ENAMEF = LIBNAM(I) + I = INDEX(FTYPE,'C') + IF(I.NE.0) ANUMTF = LIBNAM(I) + I = INDEX(FTYPE,'D') + IF(I.NE.0) ANUMHF = LIBNAM(I) + I = INDEX(FTYPE,'E') + IF(I.NE.0) BRIEFF = LIBNAM(I) + I = INDEX(FTYPE,'F') + IF(I.NE.0) KWRDTF = LIBNAM(I) + I = INDEX(FTYPE,'G') + IF(I.NE.0) KWRDHF = LIBNAM(I) + I = INDEX(FTYPE,'H') + IF(I.NE.0) AUTHTF = LIBNAM(I) + I = INDEX(FTYPE,'I') + IF(I.NE.0) AUTHHF = LIBNAM(I) + END + SUBROUTINE CDROMS(JOB,ENAME,ACNUM, + +ENAMEF,DIVLUF,ANUMTF,ANUMHF,LIBF,BRIEFF,KWRDTF,KWRDHF, + +IDEVEN,IDEVAN,IDEVDL,IDEVLF,KBIN,AUTHHF,AUTHTF, + +KBOUT,DIVCOD,SEQOFF,ANNOFF,SEQ,IDSEQ,IDEVOT, + +KEYS,SS,SE,NKEYS,LTYPE,WORKI,MAXWOR,TITLE,IOK) + CHARACTER ENAMEF*(*),DIVLUF*(*),LIBF*(*),KWRDTF*(*) + CHARACTER ANUMTF*(*),ANUMHF*(*),BRIEFF*(*),KWRDHF*(*) + CHARACTER ENAME*(*),ACNUM*(*),BARRAY*80,TERMA*5,TIT*5 + CHARACTER SEQ(IDSEQ) + INTEGER DIVCOD,ANNOFF,SEQOFF,ENTFN,RDANUM,RSIZEN,OPENFU + INTEGER CDSEQ,CDANN,CDANNT,OPENFF,SEEKRS,RDKWRD,CODSEQ + CHARACTER KEYS*(*),LTYPE*1,AUTHHF*(*),AUTHTF*(*),TITLE*(*) + INTEGER SS(NKEYS),SE(NKEYS),GENSEQ,WORKI(MAXWOR) + EXTERNAL OPENFF,SEEKRS,IENAME,RDKWRD,CODSEQ,GENSEQ + EXTERNAL ENTFN,RDANUM,IHEAD,OPENFU,CDSEQ,CDANN,CDANNT +C +C read lib data from cdrom format +C all files are opened and used as required +C job = 1 get a sequence ENAME and display its title +C job = 2 get annotation ENAME and display it +C job = 3 find entry names from accession number ACNUM +C job = 4 search author index for keywords +C job = 5 search keyword index for keywords +C job = 6 get complete entry ENAME +C +C +C LTYPE = A means embl, B means codata, C means GenBank +C + IF(LTYPE.EQ.'A') THEN + TERMA = 'SQ ' + TIT = 'DE ' + ELSE IF(LTYPE.EQ.'B') THEN + TERMA = 'SEQUE' + TIT = 'TITLE' + ELSE IF(LTYPE.EQ.'C') THEN + TERMA = 'ORIGI' + TIT = 'DEFIN' + END IF +C +C open entryname file +C + IOK = OPENFU(IDEVEN,ENAMEF) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error opening entry names index') + CALL ERROM(KBOUT,ENAMEF) + RETURN + END IF + IOK = IHEAD(IDEVEN,BARRAY,NRECEN,RSIZEN) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading entry name header') + CALL ERROM(KBOUT,ENAMEF) + RETURN + END IF + IF(JOB.EQ.1) THEN +C +C get a sequence +C +C +C open division lookup file +C + IOK = OPENFF(IDEVDL,DIVLUF) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error opening division lookup file') + CALL ERROM(KBOUT,DIVLUF) + RETURN + END IF +C +C get offsets and divcode +C + IOK = IENAME(IDEVEN,NRECEN,RSIZEN,ENAME,ANNOFF,SEQOFF, + + DIVCOD,BARRAY) + IF(IOK.NE.0) THEN + WRITE(KBOUT,*)ENAME,' not found' + RETURN + END IF +C +C get division file name +C + IOK = ENTFN(IDEVDL,DIVCOD,LIBF,BARRAY) + CLOSE(UNIT=IDEVDL) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading division lookup file') + CALL ERROM(KBOUT,DIVLUF) + RETURN + END IF +C +C open division +C + IOK = OPENFF(IDEVLF,LIBF) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error opening division file') + CALL ERROM(KBOUT,LIBF) + RETURN + END IF +C +C read seq, preceded by title +C + IOK = CDANNT(IDEVLF,ANNOFF,BARRAY,TIT) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error locating title') + RETURN + END IF + WRITE(KBOUT,*)BARRAY + TITLE = BARRAY + IOK = 99 + IF(LTYPE.EQ.'A') THEN + IOK = CDSEQ(IDEVLF,SEQOFF,SEQ,IDSEQ,LTYPE) + ELSE IF(LTYPE.EQ.'B') THEN + IOK = CODSEQ(IDEVLF,SEQOFF,SEQ,IDSEQ,LTYPE) + ELSE IF(LTYPE.EQ.'C') THEN + IOK = GENSEQ(IDEVLF,SEQOFF,SEQ,IDSEQ,LTYPE) + END IF + IF(IOK.EQ.0) RETURN + IF(IOK.EQ.2) THEN + WRITE(KBOUT,*) + + 'Only first ',IDSEQ,' characters of sequence read' + IOK = 0 + END IF + RETURN + ELSE IF(JOB.EQ.2) THEN +C +C open division lookup file +C + IOK = OPENFF(IDEVDL,DIVLUF) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error opening division lookup file') + CALL ERROM(KBOUT,DIVLUF) + RETURN + END IF +C +C get offsets and divcode +C + IOK = IENAME(IDEVEN,NRECEN,RSIZEN,ENAME,ANNOFF,SEQOFF, + + DIVCOD,BARRAY) + IF(IOK.NE.0) THEN + WRITE(KBOUT,*)ENAME,' not found' + RETURN + END IF +C +C get division file name +C + IOK = ENTFN(IDEVDL,DIVCOD,LIBF,BARRAY) + CLOSE(UNIT=IDEVDL) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading division lookup file') + CALL ERROM(KBOUT,DIVLUF) + RETURN + END IF +C +C open division +C + IOK = OPENFF(IDEVLF,LIBF) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error opening division file') + CALL ERROM(KBOUT,LIBF) + RETURN + END IF +C +C read ann +C + IOK = CDANN(IDEVLF,ANNOFF,BARRAY,IDEVOT,KBIN,KBOUT,TERMA) + IF(IOK.EQ.2) IOK = 0 + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading annotation file') + END IF + RETURN +C +C deal with accession number start point +C + ELSE IF(JOB.EQ.3) THEN +C +C read accession number +C + IOK = RDANUM(IDEVAN,ANUMTF,ANUMHF,IDEVEN,NRECEN,RSIZEN, + + ACNUM,BARRAY,ENAME,IDEVOT,KBOUT) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading accession number files') + RETURN + END IF +C +C Do author search +C + ELSE IF(JOB.EQ.4) THEN +C +C +C + MAXW2 = 1+(NRECEN-1)/32 + IF(MAXWOR/2.LT.MAXW2) THEN + CALL ERROM(KBOUT,'Too many entries for bit files') + RETURN + END IF + IOK = RDKWRD(IDEVAN,AUTHTF,AUTHHF,IDEVEN,NRECEN,RSIZEN, + + KEYS,SS,SE,NKEYS,BARRAY,ENAME,IDEVOT,KBIN,KBOUT,BRIEFF, + + WORKI,WORKI(MAXW2+1),MAXW2) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error searching author index for keywords') + RETURN + END IF +C +C Do text search +C + ELSE IF(JOB.EQ.5) THEN +C +C +C + MAXW2 = 1+(NRECEN-1)/32 + IF(MAXWOR/2.LT.MAXW2) THEN + CALL ERROM(KBOUT,'Too many entries for bit files') + RETURN + END IF + IOK = RDKWRD(IDEVAN,KWRDTF,KWRDHF,IDEVEN,NRECEN,RSIZEN, + + KEYS,SS,SE,NKEYS,BARRAY,ENAME,IDEVOT,KBIN,KBOUT,BRIEFF, + + WORKI,WORKI(MAXW2+1),MAXW2) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error searching text index for keywords') + RETURN + END IF + ELSE IF(JOB.EQ.6) THEN +C +C open division lookup file +C + IOK = OPENFF(IDEVDL,DIVLUF) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error opening division lookup file') + CALL ERROM(KBOUT,DIVLUF) + RETURN + END IF +C +C get offsets and divcode +C + IOK = IENAME(IDEVEN,NRECEN,RSIZEN,ENAME,ANNOFF,SEQOFF, + + DIVCOD,BARRAY) + IF(IOK.NE.0) THEN + WRITE(KBOUT,*)ENAME,' not found' + RETURN + END IF +C +C get division file name +C + IOK = ENTFN(IDEVDL,DIVCOD,LIBF,BARRAY) + CLOSE(UNIT=IDEVDL) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading division lookup file') + CALL ERROM(KBOUT,DIVLUF) + RETURN + END IF +C +C open division +C + IOK = OPENFF(IDEVLF,LIBF) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error opening division file') + CALL ERROM(KBOUT,LIBF) + RETURN + END IF +C +C read ann +C + IOK = CDANN(IDEVLF,ANNOFF,BARRAY,IDEVOT,KBIN,KBOUT,'//') + IF(IOK.EQ.2) IOK = 0 + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading annotation file') + END IF + RETURN + END IF + END + SUBROUTINE SRCKEY(IDEVIN,IDEVOT,KBIN,KBOUT,IOK) + CHARACTER STRING*80,NEW*80 + INTEGER J1(5),J2(5) + INTEGER NOTLR + EXTERNAL NOTLR + WRITE(KBOUT,1000) +1000 FORMAT(' Search for keywords') +10 CONTINUE + LENGTH = 0 + STRING = ' ' + NEW = ' ' + CALL GTSTR('Keywords',STRING,NEW,LENGTH,KBOUT,KBIN,INFLAG) + LENGTH = 80 + IF(INFLAG.NE.0) RETURN + STRING = NEW +C FIND SEPARATE WORDS + I = 0 + I2 = 0 +20 CONTINUE + I = I + 1 + IF(I.LT.6)THEN + I1 = I2 + 1 + LEFT = LENGTH - I1 + 1 + IF(LEFT.GT.0)THEN + IT = NOTLR(STRING(I1:LENGTH),LEFT,' ') + IF(IT.NE.0)THEN + I1 = I1 + IT - 1 + IT = INDEX(STRING(I1:LENGTH),' ') + I2 = I1 + IT - 2 + J1(I) = I1 + J2(I) = I2 + I2 = J2(I) + IF(J2(I).LT.LENGTH) GO TO 20 + END IF + GO TO 15 + END IF + GO TO 15 + END IF +15 CONTINUE + I = I - 1 + IF(I.GT.0) THEN + CALL SRCTTL(IDEVIN,IDEVOT,STRING,J1,J2,I,KBIN,KBOUT,IOK) + END IF + END + SUBROUTINE SRCKEZ(KBIN,KBOUT,STRING,J1,J2,I,PROMPT,IOK) + CHARACTER STRING*80,NEW*80,UNDER,SPACE,PROMPT*(*) + INTEGER J1(5),J2(5) + INTEGER NOTLR + EXTERNAL NOTLR + SAVE UNDER,SPACE + DATA UNDER/'_'/,SPACE/' '/ + WRITE(KBOUT,1000)PROMPT +1000 FORMAT(' Search for ',A) +10 CONTINUE + LENGTH = 0 + STRING = ' ' + NEW = ' ' + CALL GTSTR(PROMPT,STRING,NEW,LENGTH,KBOUT,KBIN,INFLAG) + LENGTH = 80 + IF(INFLAG.NE.0) RETURN + STRING = NEW +C FIND SEPARATE WORDS + I = 0 + I2 = 0 +20 CONTINUE + I = I + 1 + IF(I.LT.6)THEN + I1 = I2 + 1 + LEFT = LENGTH - I1 + 1 + IF(LEFT.GT.0)THEN + IT = NOTLR(STRING(I1:LENGTH),LEFT,' ') + IF(IT.NE.0)THEN + I1 = I1 + IT - 1 + IT = INDEX(STRING(I1:LENGTH),' ') + I2 = I1 + IT - 2 + J1(I) = I1 + J2(I) = I2 + I2 = J2(I) + IF(J2(I).LT.LENGTH) GO TO 20 + END IF + GO TO 15 + END IF + GO TO 15 + END IF +15 CONTINUE +C +C keywords are in upper case +C + CALL CCASE(STRING,1) +C +C change uinderscore to space +C + CALL EXCHNG(STRING,UNDER,SPACE) + I = I - 1 +C IF(I.GT.0) I = 1 + IOK = 0 + END + SUBROUTINE EXCHNG(STRING,FROM,TO) + CHARACTER STRING*(*),FROM,TO + DO 10 I=1,LEN(STRING) + IF (STRING(I:I).EQ.FROM) STRING(I:I) = TO + 10 CONTINUE + END + SUBROUTINE SRCTTL(IDEVIN,IDEVOT,STRING,J1,J2,NSTRNG, + +KBIN,KBOUT,IOK) + CHARACTER LINE*80, STRING*(*),NAME*10,SLEN*4,LLINE*104 + INTEGER J1(NSTRNG),J2(NSTRNG),GETRS + EXTERNAL GETRS + EQUIVALENCE (LLINE(1:1),NAME),(LLINE(25:104),LINE) + EQUIVALENCE(LLINE(21:24),SLEN) + IOK = 0 + JPAGE = 0 + IFOUND = 0 + WRITE(KBOUT,1004)STRING(1:J2(NSTRNG)) +1004 FORMAT(' Searching for ',A) + CALL CCASE(STRING,2) +1001 FORMAT(A,' ',I7,' ',A) + 1002 FORMAT(' ',A,' ',I7,' ',A) + IBYTE = 301 +10 CONTINUE + IOK = GETRS(IDEVIN,LLINE,104,IBYTE) + IF(IOK.NE.0) GO TO 30 + IBYTE = IBYTE + 104 + CALL CCASE(LINE,2) + DO 5 I = 1,NSTRNG + IF(INDEX(LINE,STRING(J1(I):J2(I))).EQ.0)GO TO 10 +5 CONTINUE + IFOUND = IFOUND + 1 + CALL BSW4(SLEN,LENS) + IF(IDEVOT.EQ.KBOUT)THEN + CALL PAGER(KBIN,KBOUT,JPAGE,IOK) + IF (IOK.NE.0) RETURN + END IF + IF(IDEVOT.EQ.KBOUT) THEN + WRITE(IDEVOT,1002,ERR=20)NAME,LENS,LINE + ELSE + WRITE(IDEVOT,1001,ERR=20)NAME,LENS,LINE + END IF + GO TO 10 +20 CONTINUE + IOK = 1 + RETURN +30 CONTINUE + WRITE(KBOUT,1003)IFOUND +1003 FORMAT(' ',I7,' entries found') + CALL BPAUSE(KBIN,KBOUT,IEX) + END + SUBROUTINE PAGER(KBIN,KBOUT,JPAGE,IOK) + PARAMETER (IPAGE = 22) + JPAGE = JPAGE + 1 + IF(JPAGE.EQ.IPAGE)THEN + CALL BPAUSE(KBIN,KBOUT,IOK) + IF(IOK.NE.0)RETURN + JPAGE = 0 + END IF + IOK = 0 + END + SUBROUTINE RDLIBL(FILNAM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IDEVLL,IDEVEN,IDEVNL, + +LIBLF,LIBIN,DIVDEV,MAXDIV,IDEVD, + +LIST,ENAMEL,LIBTYP,LTYPEP,NDIV,RSIZEN,NRECEN,IOK) +C AUTHOR RODGER STADEN +C IDEVLL = unit for library list LIBLF +C IDEVEN = entry name file ENAMEF +C IDEVD unit for division lookup file, and start for divdev + CHARACTER FILNAM*(*),LIBLF*(*) + CHARACTER HELPF*(*),LTYPEP + PARAMETER (MAXNAM = 80,LENNAM = 10) + PARAMETER (MAXPRM = 50,MAXMEN=20,MAXLIN=80) + CHARACTER PROMPT(MAXMEN)*(MAXPRM),LOGNAM(MAXMEN)*(MAXNAM) + CHARACTER FTYPE*(MAXMEN),LINE*(MAXLIN) + CHARACTER LTYPE*(MAXMEN),ENAMEL*(LENNAM) + INTEGER RSIZEN + INTEGER DIVDEV(MAXDIV) + IDIMIN = IDIM + IDIM = 0 + LIB = LIBIN +1 CONTINUE + CALL RDLB0(LTYPE,LOGNAM,PROMPT,MAXMEN,MAXNAM,MAXPRM, + +IDEVLL,LIBLF,LINE,MAXLIN,KBOUT,ITEM,IOK) + IF(IOK.NE.0) RETURN + IOK = 1 + IF(ITEM.LT.MAXMEN) THEN + ITEM = ITEM + 1 + PROMPT(ITEM) = 'Personal file in PIR format' + END IF + IF(ITEM.LT.MAXMEN) THEN + ITEM = ITEM + 1 + PROMPT(ITEM) = 'Personal file in FASTA format' + END IF + CALL RADION('Select a library',PROMPT,ITEM,LIB, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(LIB.LT.1) RETURN + IF(LIB.EQ.ITEM-1) THEN + FILNAM = ' ' + CALL OPENF1(IDEVEN,FILNAM,0,IOK,KBIN,KBOUT, + + 'Personal library in PIR format', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + LIBTYP = 2 + ELSE IF(LIB.EQ.ITEM) THEN + FILNAM = ' ' + CALL OPENF1(IDEVEN,FILNAM,0,IOK,KBIN,KBOUT, + + 'Personal library in FASTA format', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + LIBTYP = 3 + ELSE + FILNAM = LOGNAM(LIB) + CALL RDLB1(LOGNAM,MAXMEN,MAXNAM,IDEVLL,FILNAM, + + LINE,MAXLIN,FTYPE,LINENO,KBOUT,ITEM,IOK) + IF(IOK.NE.0) RETURN + LTYPEP = LTYPE(LIB:LIB) + IF(LTYPEP.EQ.'A') THEN + LIBTYP = 1 + WRITE(KBOUT,*)'Library is in EMBL format with indexes' + CALL OCDLBL(LOGNAM,MAXNAM,FTYPE,FILNAM,DIVDEV, + + MAXDIV,NDIV,IDEVEN,RSIZEN,NRECEN,IDEVD,KBOUT,IOK) + IF(IOK.NE.0) RETURN + ELSE IF(LTYPEP.EQ.'B') THEN + LIBTYP = 1 + WRITE(KBOUT,*)'Library is in CODATA format with indexes' + CALL OCDLBL(LOGNAM,MAXNAM,FTYPE,FILNAM,DIVDEV, + + MAXDIV,NDIV,IDEVEN,RSIZEN,NRECEN,IDEVD,KBOUT,IOK) + IF(IOK.NE.0) RETURN + ELSE IF(LTYPE(LIB:LIB).EQ.'C') THEN + LIBTYP = 1 + WRITE(KBOUT,*)'Library is in GenBank format with indexes' + CALL OCDLBL(LOGNAM,MAXNAM,FTYPE,FILNAM,DIVDEV, + + MAXDIV,NDIV,IDEVEN,RSIZEN,NRECEN,IDEVD,KBOUT,IOK) + IF(IOK.NE.0) RETURN + ELSE + WRITE(KBOUT,*)'Unknown library type' + IOK = 1 + RETURN + END IF + END IF +2 CONTINUE + IOPT = 1 + PROMPT(1) = 'Search whole library' + PROMPT(2) = 'Search only a list of entries' + PROMPT(3) = 'Search all but a list of entries' + CALL RADION('Select a task',PROMPT,3,IOPT, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IOPT.LT.1) GO TO 1 + IF((IOPT.EQ.2).OR.(IOPT.EQ.3)) THEN + FILNAM = ' ' + CALL OPENF1(IDEVNL,FILNAM,0,IOK,KBIN,KBOUT, + + 'File of entry names', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0)RETURN + IF(IOPT.EQ.3) THEN +C need to read in the first name for exclusion +C the rest handled by cdroml + READ(IDEVNL,1000,ERR=100,END=200)ENAMEL + 1000 FORMAT(A) + END IF + END IF + IFINEX = 0 + LIST = 0 + IF(IOPT.EQ.2) LIST = 1 + IF(IOPT.EQ.3) LIST = -1 + IOK = 0 + RETURN + 100 CONTINUE + IOK = 1 + CALL ERROM(KBOUT,'Error in names file') + RETURN + 200 CONTINUE + IOK = 2 + CALL ERROM(KBOUT,'Empty names file') + END + SUBROUTINE OCDLBL(LIBNAM,MAXLBF,FTYPE,TEMPF,DIVDEV, + +MAXDIV,NDIV,IDEVEN,RSIZEN,NRECEN,IDEVD,KBOUT,IOK) +C Assigns libnam names to file name strings, opens division lookup file, +C reads names of each division and opens the files +C seeks to first record in entry names file + CHARACTER LIBNAM(MAXLBF)*(*) + CHARACTER TEMPF*(*),BARRAY*10 + CHARACTER FTYPE*(*) + INTEGER DIVDEV(MAXDIV),RSIZEN + INTEGER OPENFU,IHEAD,OPENFF + EXTERNAL OPENFU,IHEAD,OPENFF + TEMPF = ' ' + TEMPF = LIBNAM(INDEX(FTYPE,'A')) + IOK = OPENFF(IDEVD,TEMPF) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error opening division lookup file') + CALL ERROM(KBOUT,TEMPF) + RETURN + END IF + IOK = 2 + NDIV = 0 +C use division as additions to idevd + 10 READ(IDEVD,1000,ERR=200,END=100)IDIV,TEMPF + 1000 FORMAT(I6,1X,A) + IF((IDIV.GT.0).AND.(NDIV.LT.MAXDIV)) THEN + NDIV = NDIV + 1 + DIVDEV(NDIV) = IDEVD + IDIV + IF(OPENFF(DIVDEV(NDIV),TEMPF).NE.0) THEN + CALL ERROM(KBOUT,'Error opening division file') + CALL ERROM(KBOUT,TEMPF) + RETURN + END IF + GO TO 10 + END IF + IOK = 5 + RETURN + 100 CONTINUE + CLOSE(UNIT=IDEVD) + IOK = 3 + TEMPF = LIBNAM(INDEX(FTYPE,'B')) + IF(OPENFU(IDEVEN,TEMPF).NE.0) THEN + CALL ERROM(KBOUT,'Error opening entryname index') + CALL ERROM(KBOUT,TEMPF) + RETURN + END IF + IOK = 5 + IF(IHEAD(IDEVEN,BARRAY,NRECEN,RSIZEN).NE.0) THEN + CALL ERROM(KBOUT,'Error reading entryname index header') + CALL ERROM(KBOUT,TEMPF) + RETURN + END IF + IOK = 0 + RETURN + 200 CONTINUE + CALL ERROM(KBOUT,'Error reading division file') + IOK = 4 + END + SUBROUTINE CDROML(JOB,ENAME,ENAMEL, + +IDEVEN,RSIZEN,NRECEN,IDEVNL,SEQ,IDSEQ, + +DIVDEV,NDIV,ICREC,IFIN,TITLE,KBOUT,LTYPE,IOK) + CHARACTER ENAME*(*),ENAMEL*(*),BARRAY*80,TITLE*(*) + CHARACTER SEQ(IDSEQ),LTYPE,TIT*5 + INTEGER DIVCOD,ANNOFF,SEQOFF,RSIZEN,DIVDEV(NDIV) + INTEGER CDSEQ,CDANNT,GNEXTN,CODSEQ,GENSEQ + EXTERNAL IENAME + EXTERNAL CDSEQ,CDANNT,GNEXTN,CODSEQ,GENSEQ +C +C read lib data from cdrom format +C +C all files are already open +C the entry names files is poised on the first entry, when we come in +C first time. Records are numbered 0 to nrecen. +C +C job = 0 process whole library, entry name by entryname +C 1 process a list of entry names +C -1 process all but a list of entry names +C 2 get a single entry name +C +C error returns +C iok = 0 ok +C -1 not found +C -2 error +C -3 end +C +C LTYPE = A means embl, B means codata, C means GenBank +C + IF (ICREC.EQ.NRECEN) THEN + IOK = -3 + CALL ERROM(KBOUT,'End of library reached') + RETURN + END IF + IF(LTYPE.EQ.'A') THEN + TIT = 'DE ' + ELSE IF(LTYPE.EQ.'B') THEN + TIT = 'TITLE' + ELSE IF(LTYPE.EQ.'C') THEN + TIT = 'DEFIN' + END IF + IF(JOB.EQ.0) THEN +C +C get next sequence in entry name index +C +C get offsets and divcode (error as for getrs) +C + IOK = GNEXTN(IDEVEN,RSIZEN,ENAME,ANNOFF,SEQOFF,DIVCOD, + + BARRAY,ICREC) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading entryname index') + RETURN + END IF +C +C read seq, preceded by title +C + IOK = CDANNT(DIVDEV(DIVCOD),ANNOFF,BARRAY,TIT) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading title') + RETURN + END IF + TITLE = BARRAY + IOK = 99 + IF(LTYPE.EQ.'A') THEN + IOK = CDSEQ(DIVDEV(DIVCOD),SEQOFF,SEQ,IDSEQ) + ELSE IF(LTYPE.EQ.'B') THEN + IOK = CODSEQ(DIVDEV(DIVCOD),SEQOFF,SEQ,IDSEQ) + ELSE IF(LTYPE.EQ.'C') THEN + IOK = GENSEQ(DIVDEV(DIVCOD),SEQOFF,SEQ,IDSEQ) + END IF + IF(IOK.EQ.0) RETURN + IF(IOK.EQ.2) THEN + WRITE(KBOUT,*) + + 'Only first ',IDSEQ,' characters of sequence read' + IOK = 0 + RETURN + END IF + CALL ERROM(KBOUT,'Error reading sequence') + RETURN + ELSE IF(JOB.EQ.2) THEN +C +C get entry name +C +C +C get offsets and divcode +C + IOK = IENAME(IDEVEN,NRECEN,RSIZEN,ENAME,ANNOFF,SEQOFF, + + DIVCOD,BARRAY) + IF(IOK.NE.0) THEN + WRITE(KBOUT,*)ENAME,' not found' + RETURN + END IF +C +C read seq, preceded by title +C + IOK = CDANNT(DIVDEV(DIVCOD),ANNOFF,BARRAY,TIT) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading title') + RETURN + END IF + TITLE = BARRAY + IOK = 99 + IF(LTYPE.EQ.'A') THEN + IOK = CDSEQ(DIVDEV(DIVCOD),SEQOFF,SEQ,IDSEQ) + ELSE IF(LTYPE.EQ.'B') THEN + IOK = CODSEQ(DIVDEV(DIVCOD),SEQOFF,SEQ,IDSEQ) + ELSE IF(LTYPE.EQ.'C') THEN + IOK = GENSEQ(DIVDEV(DIVCOD),SEQOFF,SEQ,IDSEQ) + END IF + IF(IOK.EQ.0) RETURN + IF(IOK.EQ.2) THEN + WRITE(KBOUT,*) + + 'Only first ',IDSEQ,' characters of sequence read' + IOK = 0 + RETURN + END IF + CALL ERROM(KBOUT,'Error reading sequence') + RETURN + ELSE IF(JOB.EQ.1) THEN +C +C get next sequence in entry name list +C + 90 CONTINUE + READ(IDEVNL,1000,ERR=100,END=200)ENAME +C +C get offsets and divcode +C + IOK = IENAME(IDEVEN,NRECEN,RSIZEN,ENAME,ANNOFF,SEQOFF, + + DIVCOD,BARRAY) + IF(IOK.NE.0) THEN + WRITE(KBOUT,*)ENAME,' not found' + GO TO 90 + END IF +C +C read seq, preceded by title +C + IOK = CDANNT(DIVDEV(DIVCOD),ANNOFF,BARRAY,TIT) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading title') + RETURN + END IF + TITLE = BARRAY + IOK = 99 + IF(LTYPE.EQ.'A') THEN + IOK = CDSEQ(DIVDEV(DIVCOD),SEQOFF,SEQ,IDSEQ) + ELSE IF(LTYPE.EQ.'B') THEN + IOK = CODSEQ(DIVDEV(DIVCOD),SEQOFF,SEQ,IDSEQ) + ELSE IF(LTYPE.EQ.'C') THEN + IOK = GENSEQ(DIVDEV(DIVCOD),SEQOFF,SEQ,IDSEQ) + END IF + IF(IOK.EQ.0) RETURN + IF(IOK.EQ.2) THEN + WRITE(KBOUT,*) + + 'Only first ',IDSEQ,' characters of sequence read' + IOK = 0 + RETURN + END IF + CALL ERROM(KBOUT,'Error reading sequence') + RETURN + 100 CONTINUE + IOK = -3 + CALL ERROM(KBOUT,'Error reading entrynames list') + RETURN + 200 CONTINUE + IOK = -1 + RETURN + ELSE IF(JOB.EQ.-1) THEN +C +C get offsets and divcode +C + 250 CONTINUE + IOK = GNEXTN(IDEVEN,RSIZEN,ENAME,ANNOFF,SEQOFF,DIVCOD, + + BARRAY,ICREC) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading entryname index') + RETURN + END IF +C +C is it the same as the one on the list? +C + IF(ENAMEL.EQ.ENAME) THEN + IF(IFIN.EQ.0) THEN + READ(IDEVNL,1000,ERR=300,END=400)ENAMEL + 1000 FORMAT(A) + END IF + GO TO 250 + END IF +C +C read seq, preceded by title +C + IOK = CDANNT(DIVDEV(DIVCOD),ANNOFF,BARRAY,TIT) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error reading title') + RETURN + END IF + TITLE = BARRAY + IOK = 99 + IF(LTYPE.EQ.'A') THEN + IOK = CDSEQ(DIVDEV(DIVCOD),SEQOFF,SEQ,IDSEQ) + ELSE IF(LTYPE.EQ.'B') THEN + IOK = CODSEQ(DIVDEV(DIVCOD),SEQOFF,SEQ,IDSEQ) + ELSE IF(LTYPE.EQ.'C') THEN + IOK = GENSEQ(DIVDEV(DIVCOD),SEQOFF,SEQ,IDSEQ) + END IF + IF(IOK.EQ.0) RETURN + IF(IOK.EQ.2) THEN + WRITE(KBOUT,*) + + 'Only first ',IDSEQ,' characters of sequence read' + IOK = 0 + RETURN + END IF + CALL ERROM(KBOUT,'Error reading sequence') + RETURN + 300 CONTINUE + IOK = -3 + CALL ERROM(KBOUT,'Error reading entrynames list') + RETURN + 400 CONTINUE +C +C end of exclude list reached, so include the rest +C + IFIN = 1 + ENAMEL = ' ' + GO TO 250 + END IF + END + INTEGER FUNCTION IENAME(IDEVEN,NREC,RSIZE, + +ENAME,ANNOFF,SEQOFF,DIVCOD,BARRAY) + INTEGER ANNOFF,SEQOFF,DIVCOD,GETRS,NREC,RSIZE,SEEKRS + INTEGER UB,B1,BEND + INTEGER*2 STAR2 + CHARACTER BARRAY*(*),ENAME*(*) + EXTERNAL GETRS,SEEKRS + B1 = 0 + BEND = NREC + LB = B1 + UB = BEND + 20 CONTINUE + IF(UB.LT.LB) THEN + IENAME = -1 + RETURN + END IF + IREC = (LB+UB)/2 + IBYTE = 301 + RSIZE*IREC + IENAME = GETRS(IDEVEN,BARRAY,20,IBYTE) + IF(IENAME.NE.0) RETURN + IF(ENAME.LT.BARRAY(1:10)) THEN + UB = IREC - 1 + ELSE IF(ENAME.GT.BARRAY(1:10)) THEN + LB = IREC + 1 + ELSE + CALL BSW4(BARRAY(11:),ANNOFF) + CALL BSW4(BARRAY(15:),SEQOFF) + CALL BSW2(BARRAY(19:),STAR2) + DIVCOD = STAR2 + RETURN + END IF + GO TO 20 + END + INTEGER FUNCTION CDSEQ(IDEV,SEQOFF,SEQ,IDSEQ) +C seek to seq and return it + CHARACTER SEQ(IDSEQ),LT + CHARACTER CR + PARAMETER (CR=CHAR(13)) + INTEGER SEQOFF + INTEGER SEEKRS + EXTERNAL SEEKRS + I = SEQOFF - 5 + CDSEQ = SEEKRS(IDEV,I) + IF(CDSEQ.NE.0) RETURN + NSEQ = 0 + 10 CONTINUE + LSEQ = MIN(NSEQ+60,IDSEQ) + READ(IDEV,1000,ERR=100,END=200)LT,(SEQ(K),K=NSEQ+1,LSEQ) + 1000 FORMAT(A,4X,6(10A1,1X)) + IF(LT.EQ.'/') THEN + CDSEQ = 0 +C +C seq must have ended on previous line +C so look for carriage return +C + DO 20 I=NSEQ-59,NSEQ + IF(SEQ(I).EQ.CR) THEN + J = I-1 + GO TO 21 + END IF + 20 CONTINUE + J = NSEQ + 21 CONTINUE +C +C now look for a space +C + DO 30 I=NSEQ-59,J + IF(SEQ(I).EQ.' ') THEN + IDSEQ = I-1 + RETURN + END IF + 30 CONTINUE + IDSEQ = J + RETURN + END IF + NSEQ = LSEQ + IF(NSEQ.LT.IDSEQ) GO TO 10 + CDSEQ = 2 + RETURN + 100 CONTINUE + WRITE(*,*)'ERROR IN CDSEQ' + CDSEQ = -2 + RETURN + 200 CONTINUE + WRITE(*,*)'END IN CDSEQ' + CDSEQ = -1 + END + INTEGER FUNCTION GENSEQ(IDEV,SEQOFF,SEQ,IDSEQ) +C seek to seq and return it + CHARACTER SEQ(IDSEQ),LT + CHARACTER CR + PARAMETER (CR=CHAR(13)) + INTEGER SEQOFF + INTEGER SEEKRS + EXTERNAL SEEKRS +C +C seek to beginning of line containing first seq data +C + I = SEQOFF - 10 + GENSEQ = SEEKRS(IDEV,I) + IF(GENSEQ.NE.0) RETURN + NSEQ = 0 + 10 CONTINUE + LSEQ = MIN(NSEQ+60,IDSEQ) + READ(IDEV,1000,ERR=100,END=200)LT,(SEQ(K),K=NSEQ+1,LSEQ) + 1000 FORMAT(A,9X,6(10A1,1X)) + IF(LT.EQ.'/') THEN + GENSEQ = 0 +C +C seq must have ended on previous line +C so look for carriage return +C + DO 20 I=NSEQ-59,NSEQ + IF(SEQ(I).EQ.CR) THEN + J = I-1 + GO TO 21 + END IF + 20 CONTINUE + J = NSEQ + 21 CONTINUE +C +C now look for a space +C + DO 30 I=NSEQ-59,J + IF(SEQ(I).EQ.' ') THEN + IDSEQ = I-1 + RETURN + END IF + 30 CONTINUE + IDSEQ = J + RETURN + END IF + NSEQ = LSEQ + IF(NSEQ.LT.IDSEQ) GO TO 10 + GENSEQ = 2 + RETURN + 100 CONTINUE + WRITE(*,*)'ERROR IN GENSEQ' + GENSEQ = -2 + RETURN + 200 CONTINUE + WRITE(*,*)'END IN GENSEQ' + GENSEQ = -1 + END + INTEGER FUNCTION CODSEQ(IDEV,SEQOFF,SEQ,IDSEQ) +C seek to seq and return it + CHARACTER SEQ(IDSEQ),LT + CHARACTER CR + PARAMETER (CR=CHAR(13)) + INTEGER SEQOFF + INTEGER SEEKRS + EXTERNAL SEEKRS +C +C seek to beginning of line containing first seq data +C + I = SEQOFF - 8 + CODSEQ = SEEKRS(IDEV,I) + IF(CODSEQ.NE.0) RETURN + NSEQ = 0 + 10 CONTINUE + LSEQ = MIN(NSEQ+30,IDSEQ) + READ(IDEV,1000,ERR=100,END=200)LT,(SEQ(K),K=NSEQ+1,LSEQ) + 1000 FORMAT(A,7X,30(A1,1X)) + IF(LT.EQ.'/') THEN + CODSEQ = 0 +C +C seq must have ended on previous line +C so look for carriage return +C + DO 20 I=NSEQ-29,NSEQ + IF(SEQ(I).EQ.CR) THEN + J = I-1 + GO TO 21 + END IF + 20 CONTINUE + J = NSEQ + 21 CONTINUE +C +C now look for a space +C + DO 30 I=NSEQ-29,J + IF(SEQ(I).EQ.' ') THEN + IDSEQ = I-1 + RETURN + END IF + 30 CONTINUE + IDSEQ = J + RETURN + END IF + NSEQ = LSEQ + IF(NSEQ.LT.IDSEQ) GO TO 10 + CODSEQ = 2 + RETURN + 100 CONTINUE + WRITE(*,*)'ERROR IN CODSEQ' + CODSEQ = -2 + RETURN + 200 CONTINUE + WRITE(*,*)'END IN CODSEQ' + CODSEQ = -1 + END + INTEGER FUNCTION CDANN(IDEV,ANNOFF,BARRAY,IDEVOT,KBIN,KBOUT,TERM) + CHARACTER BARRAY*(*),TERM*(*) + INTEGER ANNOFF + INTEGER SEEKRS,GETRSL + EXTERNAL SEEKRS,GETRSL + CDANN = SEEKRS(IDEV,ANNOFF) + IF(CDANN.NE.0) RETURN + JPAGE = 0 + 10 CONTINUE + IOK = GETRSL(IDEV,BARRAY,80) + IF(IOK.LT.1) THEN + CDANN = IOK + RETURN + END IF + IF(IDEVOT.EQ.KBOUT) THEN + CALL PAGER(KBIN,KBOUT,JPAGE,JOK) + IF (JOK.NE.0) THEN + CDANN = 2 + RETURN + END IF + END IF + IF (IDEVOT.EQ.KBOUT) THEN + WRITE(IDEVOT,1000)BARRAY(1:IOK) + ELSE + WRITE(IDEVOT,1001)BARRAY(1:IOK) + END IF + IF(BARRAY(1:LEN(TERM)).NE.TERM) GO TO 10 + CDANN = 0 + 1000 FORMAT(' ',A) + 1001 FORMAT(A) + END + INTEGER FUNCTION CDANNT(IDEV,ANNOFF,BARRAY,TIT) + CHARACTER BARRAY*(*),TIT*(*) + INTEGER ANNOFF + INTEGER SEEKRS,GETRSL + EXTERNAL SEEKRS,GETRSL + CDANNT = SEEKRS(IDEV,ANNOFF) + IF(CDANNT.NE.0) RETURN + 10 CONTINUE + IOK = GETRSL(IDEV,BARRAY,80) + IF(IOK.LT.1) THEN + CDANNT = IOK + RETURN + END IF + IF(BARRAY(1:LEN(TIT)).NE.TIT) GO TO 10 + CDANNT = 0 + IF(IOK.LT.80) BARRAY(IOK+1:80) = ' ' + END + INTEGER FUNCTION RDANUM(IDEVAN,ANUMTF,ANUMHF,IDEVEN, + +NRECEN,RSIZEN,ACNUM,BARRAY,ENAME,IDEVOT,KBOUT) + CHARACTER ANUMTF*(*),ANUMHF*(*),ACNUM*(*),BARRAY*(*) + CHARACTER ENAME*(*) + INTEGER OPENFU,ACNUMP,GETRS,ACNUMQ + INTEGER ENAMEP,RSIZAN,RSIZEN + EXTERNAL IHEAD,OPENFU,GETRS,IANUM +C start with accession number +C sent an accesion number acnum, return list of entry names +C +C open acnum.trg, +C read until acnum found, get accession number pointer ACNUMP, number of hits +C NHITS +C close acnum.trg +C open acnum.hit, seek to ACNUMP +C read a record, write to screen, for each of NHITS +C close acnum.hit +C +C open accession number target file +C +C WRITE(*,*)'OPENING' +C WRITE(*,*)ANUMTF + RDANUM = OPENFU(IDEVAN,ANUMTF) + IF(RDANUM.NE.0) THEN + CALL ERROM(KBOUT,'Error opening target file') + CALL ERROM(KBOUT,ANUMTF) + RETURN + END IF +C WRITE(*,*)'OPEN' +C +C read its header +C +C WRITE(*,*)'READING' + RDANUM = IHEAD(IDEVAN,BARRAY,NRECAN,RSIZAN) + IF(RDANUM.NE.0) RETURN +C WRITE(*,*)'NRECAN,RSIZAN',NRECAN,RSIZAN +C WRITE(*,*)'READ' +C +C get the number of hits and the record number of the first hit +C + RDANUM = IANUM(IDEVAN,ACNUM,NRECAN,RSIZAN, + + NHITS,ACNUMP,BARRAY) + CLOSE(UNIT=IDEVAN) +C WRITE(*,*)'NHITS,acnump',NHITS,ACNUMP + IF(NHITS.LT.1) THEN + WRITE(IDEVOT,*)ACNUM,' not found' + RETURN + END IF + IF(RDANUM.NE.0) RETURN +C +C open the accession number hit file +C +C WRITE(*,*)'READING' + RDANUM = OPENFU(IDEVAN,ANUMHF) + IF(RDANUM.NE.0) THEN + CALL ERROM(KBOUT,'Error opening hit file') + CALL ERROM(KBOUT,ANUMHF) + RETURN + END IF +C WRITE(*,*)'READ' +C +C the records are 4 bytes and follow a 300 byte header +C + DO 10 I = 1,NHITS + ACNUMQ = 301 + (ACNUMP-1)*4 + RDANUM = GETRS(IDEVAN,BARRAY,4,ACNUMQ) + IF(RDANUM.NE.0) RETURN + CALL BSW4(BARRAY(1:),ENAMEP) +C WRITE(*,*)'ENAMEP',ENAMEP +C +C the entry name file has records of size rsizen and the usual 300 byte header +C + ENAMEP = 301 + (ENAMEP-1)*RSIZEN + RDANUM = GETRS(IDEVEN,BARRAY,RSIZEN,ENAMEP) + IF(RDANUM.NE.0) RETURN + ENAME = BARRAY(1:10) + WRITE(IDEVOT,*)'Entry name ',ENAME +C bug fix 18-10-91: added next line + ACNUMP = ACNUMP + 1 + 10 CONTINUE + CLOSE(UNIT=IDEVAN) + END + INTEGER FUNCTION IANUM(IDEV,ACNUM,NREC,RSIZE, + +NHITS,ACNUMP,BARRAY) + INTEGER GETRS,NREC,RSIZE,ACNUMP + INTEGER UB,B1,BEND + CHARACTER BARRAY*(*),ACNUM*(*) + EXTERNAL GETRS +C given an accession number acnum, does binary search. +C returns the number of hits NHITS and the record number of the first hit ACNUMP + NHITS = 0 + B1 = 0 + BEND = NREC + 10 CONTINUE + LB = B1 + UB = BEND + 20 CONTINUE + IF(UB.LT.LB) THEN + IANUM = 0 + RETURN + END IF + IREC = (LB+UB)/2 + IBYTE = 301 + RSIZE*IREC +C WRITE(*,*)IBYTE + IANUM = GETRS(IDEV,BARRAY,RSIZE,IBYTE) +C WRITE(*,*)BARRAY(9:18) + IF(IANUM.NE.0) RETURN + IF(ACNUM.LT.BARRAY(9:18)) THEN + UB = IREC - 1 + ELSE IF(ACNUM.GT.BARRAY(9:18)) THEN + LB = IREC + 1 + ELSE + CALL BSW4(BARRAY(1:),NHITS) + CALL BSW4(BARRAY(5:),ACNUMP) + RETURN + END IF + GO TO 20 + END + INTEGER FUNCTION RDKWRD(IDEVKW,KWRDTF,KWRDHF,IDEVEN, + +NRECEN,RSIZEN,KEYS,SS,SE,NKEYS,BARRAY,ENAME,IDEVOT, + +KBIN,KBOUT,BRIEFF,BITAR0,BITAR1,MAXWRD) + CHARACTER KWRDTF*(*),KWRDHF*(*),KEYS*(*),BARRAY*(*) + CHARACTER ENAME*(*),BRIEFF*(*) + INTEGER OPENFU,GETRS + INTEGER ENAMEP,RSIZKW,RSIZEN + INTEGER BITAR0(0:MAXWRD),BITAR1(0:MAXWRD) + INTEGER NHITS(5),KWRDP(5),SS(NKEYS),SE(NKEYS) + EXTERNAL IHEAD,OPENFU,GETRS,IKWRD + RDKWRD = 1 +C MAXWRD = (NRECEN-1)/32 +C start with keywords in KEYS +C sent nkeys keywords, return list of entry names +C +C open keyword.trg, +C read until strings found, get keyword record pointer KWRDP, +C number of hits NHITS +C close KEYWORD.trg +C open KEYWORD.hit, seek to KWRDP +C read a record, write to screen, for each of NHITS +C close KEYWORD.hit + IFOUND = 0 +C +C open KEYWORD target file +C + RDKWRD = OPENFU(IDEVKW,KWRDTF) + IF(RDKWRD.NE.0) THEN + CALL ERROM(KBOUT,'Error opening target file') + CALL ERROM(KBOUT,KWRDTF) + RETURN + END IF +C +C read its header +C + RDKWRD = IHEAD(IDEVKW,BARRAY,NRECKW,RSIZKW) + IF(RDKWRD.NE.0) THEN + CLOSE(IDEVKW) + RETURN + END IF +C +C get start record number and nhits for each string +C + DO 10 I=1,NKEYS +C +C get the number of hits and the record number of the first hit +C + LS = SE(I) - SS(I) + 1 + RDKWRD = IKWRD(IDEVKW,KEYS(SS(I):),LS,NRECKW,RSIZKW, + + NHITS(I),KWRDP(I),BARRAY) + IF(NHITS(I).LT.1) THEN + WRITE(IDEVOT,*)KEYS(SS(I):SE(I)),' not found' + CLOSE(UNIT=IDEVKW) + RETURN + END IF + IF(RDKWRD.NE.0) THEN + CLOSE(UNIT=IDEVKW) + RETURN + END IF + WRITE(IDEVOT,*)KEYS(SS(I):SE(I)),' hits',NHITS(I) + 10 CONTINUE + CLOSE(UNIT=IDEVKW) + CALL BPAUSE(KBIN,KBOUT,IEX) + IF(IEX.NE.0)RETURN +C +C open the KEYWORD hit file +C + RDKWRD = OPENFU(IDEVKW,KWRDHF) + IF(RDKWRD.NE.0) THEN + CALL ERROM(KBOUT,'Error opening hit file') + CALL ERROM(KBOUT,KWRDHF) + RETURN + END IF +C +C the records are 4 bytes and follow a 300 byte header +C +C For each hit for keyword 1 find its entryname record pointer and +C then find the entryname record pointers for each hit for each of +C the other keywords. +C +C kwrdp(1) is the number of the record in the hit file that contains +C the first hit for the first keyword +C WRITE(*,*)'NHITS(1)',NHITS(1) +C +C new stuff: clear bit arrays, set bits in bitar0 for first word +C set bits in bitar1 for other words, then and/or them with array0 + CALL CABIT(BITAR0,MAXWRD) + KWPR = KWRDP(1) + DO 40 I = 1,NHITS(1) + KWP = 301 + (KWPR-1)*4 +C add to header and then read the entrynum of the first matching entry + RDKWRD = GETRS(IDEVKW,BARRAY,4,KWP) + IF(RDKWRD.NE.0) THEN + CLOSE(UNIT=IDEVKW) + RETURN + END IF + CALL BSW4(BARRAY(1:),ENAMEP) +C enamep is the record number of the first matching entry +C so set the corresponding bit + CALL SABIT(BITAR0,MAXWRD,ENAMEP) + KWPR = KWPR + 1 + 40 CONTINUE +C +C now do the other keywords +C + DO 25 J = 2,NKEYS + CALL CABIT(BITAR1,MAXWRD) + KWPR = KWRDP(J) + DO 30 I=1,NHITS(J) + KWP = 301 + (KWPR-1)*4 + RDKWRD = GETRS(IDEVKW,BARRAY,4,KWP) + IF(RDKWRD.NE.0) THEN + CLOSE(UNIT=IDEVKW) + RETURN + END IF + CALL BSW4(BARRAY(1:),ENAMEP) +C enamep is the record number of the first matching entry +C so set the corresponding bit + CALL SABIT(BITAR1,MAXWRD,ENAMEP) + KWPR = KWPR + 1 + 30 CONTINUE +C +C and the bits with bitar0 +C + CALL AAWORD(BITAR0,BITAR1,MAXWRD) + 25 CONTINUE + CLOSE(UNIT=IDEVKW) +C +C open brief directory file +C + IOK = OPENFU(IDEVKW,BRIEFF) + IF(IOK.NE.0) THEN + CALL ERROM(KBOUT,'Error opening brief directory file') + CALL ERROM(KBOUT,BRIEFF) + RETURN + END IF + CALL TBITAL(BITAR0,MAXWRD,IDEVKW,IDEVOT,KBIN,KBOUT,ENAME) + CLOSE(UNIT=IDEVKW) + END + SUBROUTINE TBITAO(BITAR,MAXENT,IDEVEN,BARRAY,RSIZEN,IDEVOT, + +ENAME) +C old routine + CHARACTER BARRAY*(*) + CHARACTER ENAME*(*) + LOGICAL TBITRS + INTEGER GETRS,BITAR(0:MAXENT) + INTEGER ENAMEP,RSIZEN + EXTERNAL GETRS,TBITRS +C +C the entry name file has records of size rsizen and the usual 300 byte header +C + IFOUND = 0 + DO 10 I =0,MAXENT + DO 5 J =0,31 + IF(TBITRS(BITAR(I),J)) THEN + ENAMEP = I*32 + 1 + MOD(J,32) + ENAMEP = 301 + (ENAMEP-1)*RSIZEN + IOK = GETRS(IDEVEN,BARRAY,RSIZEN,ENAMEP) + IF(IOK.NE.0) THEN + RETURN + END IF + ENAME = BARRAY(1:10) + IFOUND = IFOUND + 1 + WRITE(IDEVOT,*)ENAME,IFOUND + END IF + 5 CONTINUE + 10 CONTINUE + END + SUBROUTINE TBITAL(BITAR,MAXENT,IDEVIN,IDEVOT,KBIN,KBOUT,ENAME) + LOGICAL TBITRS + INTEGER GETRS,BITAR(0:MAXENT) + EXTERNAL GETRS,TBITRS,NOTIRL + CHARACTER LINE*80,NAME*10,ACNUM*10,SLEN*4,LLINE*104,ENAME*(*) + EQUIVALENCE (LLINE(1:1),NAME),(LLINE(25:104),LINE) + EQUIVALENCE(LLINE(21:24),SLEN),(LLINE(11:20),ACNUM) +C +C the entry name file has records of size 104 and the usual 300 byte header +C + IFOUND = 0 + DO 4 I =0,MAXENT + IF (BITAR(I).NE.0) THEN + DO 3 J =0,31 + IF(TBITRS(BITAR(I),J)) IFOUND = IFOUND + 1 + 3 CONTINUE + END IF + 4 CONTINUE + WRITE(KBOUT,1004)IFOUND + 1004 FORMAT(' Different entries ',I7) + IFOUND = 0 + JPAGE = 0 + DO 10 I =0,MAXENT + IF (BITAR(I).NE.0) THEN + DO 5 J =0,31 + IF(TBITRS(BITAR(I),J)) THEN + IBYTE = I*32 + 1 + MOD(J,32) + IBYTE = 301 + (IBYTE-1)*104 + IOK = GETRS(IDEVIN,LLINE,104,IBYTE) + IF(IOK.NE.0) THEN + RETURN + END IF + CALL BSW4(SLEN,LENS) + IF(IDEVOT.EQ.KBOUT)THEN + CALL PAGER(KBIN,KBOUT,JPAGE,IOK) + IF (IOK.NE.0) RETURN + END IF + ENAME = NAME + LINEND = NOTIRL(LINE,80,' ') + IF(IDEVOT.EQ.KBOUT) THEN + WRITE(IDEVOT,1002,ERR=20) + + NAME,ACNUM,LENS,LINE(1:LINEND) + ELSE + WRITE(IDEVOT,1001,ERR=20) + + NAME,ACNUM,LENS,LINE(1:LINEND) + 1001 FORMAT(A,' ',A,' ',I7,' ',A) + 1002 FORMAT(' ',A,' ',A,' ',I7,' ',A) + END IF + IFOUND = IFOUND + 1 + END IF + 5 CONTINUE + END IF + 10 CONTINUE + 20 CONTINUE + WRITE(KBOUT,1003)IFOUND +1003 FORMAT(' ',I7,' different entries found') + CALL BPAUSE(KBIN,KBOUT,IEX) + END + INTEGER FUNCTION IKWRD(IDEV,KEYW,LS,NREC,RSIZE, + +NHITS,KWRDP,BARRAY) + INTEGER GETRS,NREC,RSIZE + INTEGER UB,B1,BEND + CHARACTER BARRAY*(*),KEYW*(*) + EXTERNAL GETRS,IKWRDB +C given a KEYWORD KEYW OF LENGTH LSS, does binary search. +C returns the number of hits NHITS and the record number of the first hit KWRDP +C Note i make the assumption that the hit file will be ordered +C so that say all words beginning with sugar follow one another +C without interuption and in order. If not use the following: +C set string length to lss + 1 so we know the string found +C ends with a space: we wont find sugars if we search for sugar +C LS = LSS + 1 + NHITS = 0 + B1 = 0 + BEND = NREC + 10 CONTINUE + LB = B1 + UB = BEND + 20 CONTINUE + IF(UB.LT.LB) THEN + IKWRD = 0 + RETURN + END IF + IREC = (LB+UB)/2 + IBYTE = 301 + RSIZE*(IREC-1) +C WRITE(*,*)'IBYTE',IBYTE + IKWRD = GETRS(IDEV,BARRAY,RSIZE,IBYTE) + IF(IKWRD.NE.0) RETURN + IF(KEYW(1:LS).LT.BARRAY(9:8+LS)) THEN + UB = IREC - 1 + ELSE IF(KEYW(1:LS).GT.BARRAY(9:8+LS)) THEN + LB = IREC + 1 + ELSE +C a hit but it may not be the first! so look back until not a hit +C then go forward accumulating a count of hits until a nonmatch is found +C next 2 lines instead of call to ikwrdb if assumption mentioned above +C does not hold +C CALL BSW4(BARRAY(5:),KWRDP) +C CALL BSW4(BARRAY(1:),NHITS) + IKWRD = IKWRDB(IDEV,KEYW,LS,NREC,RSIZE, + + NHITS,KWRDP,BARRAY,IREC) +C WRITE(*,*)'NHITS,KWRDP,IKWRD',NHITS,KWRDP,IKWRD + RETURN + END IF + GO TO 20 + END + INTEGER FUNCTION IKWRDB(IDEV,KEYW,LS,NREC,RSIZE, + +NHITS,KWRDP,BARRAY,IREC) + INTEGER GETRS,NREC,RSIZE + CHARACTER BARRAY*(*),KEYW*(*) + EXTERNAL GETRS +C given a KEYWORD KEYW OF LENGTH LS, and a start record with +C a hit but it may not be the first! so look back until not a hit +C then go forward accumulating a count of hits until a nonmatch is found + NHITS = 0 + 10 CONTINUE +C WRITE(*,*)'IREC IN IKWRDB',IREC +C SHOULD THIS BE 0 OR 1? + IREC = IREC - 1 + IF(IREC.LT.0) THEN + IKWRDB = 0 + RETURN + END IF + IBYTE = 301 + RSIZE*(IREC-1) + IKWRDB = GETRS(IDEV,BARRAY,RSIZE,IBYTE) + IF(IKWRDB.NE.0) RETURN + IF(KEYW(1:LS).EQ.BARRAY(9:8+LS)) GO TO 10 +C found first occurrence + IREC = IREC + 1 + IBYTE = 301 + RSIZE*(IREC-1) + IKWRDB = GETRS(IDEV,BARRAY,RSIZE,IBYTE) + IF(IKWRDB.NE.0) RETURN + CALL BSW4(BARRAY(5:),KWRDP) + 20 CONTINUE + CALL BSW4(BARRAY(1:),JHITS) +C WRITE(*,*)'JHITS,NHITS',JHITS,NHITS + NHITS = NHITS + JHITS + IREC = IREC + 1 +C WRITE(*,*)'IREC IN IKWRDB 2',IREC + IF(IREC.GT.NREC) THEN + IKWRDB = 0 + RETURN + END IF + IBYTE = 301 + RSIZE*(IREC-1) +C WRITE(*,*)'IBYTE IN IKWRDB',IBYTE + IKWRDB = GETRS(IDEV,BARRAY,RSIZE,IBYTE) + IF(IKWRDB.NE.0) RETURN + IF(KEYW(1:LS).EQ.BARRAY(9:8+LS)) GO TO 20 +C WRITE(*,*)'OFF END IN KWRDB' + END + INTEGER FUNCTION GNEXTN(IDEVEN,RSIZE, + +ENAME,ANNOFF,SEQOFF,DIVCOD,BARRAY,ICREC) +C get next entry name from entrynam.idx leaving pointer at next name + INTEGER ANNOFF,SEQOFF,DIVCOD,GETRS,RSIZE + INTEGER*2 STAR2 + CHARACTER BARRAY*(*),ENAME*(*) + EXTERNAL GETRS +C this routine numbers records 0 to nrec-1 (unlike all the others!!!!) +C sipl,nipl,pipl would need irec = 1 if we make this consistent and use irec-1 + IBYTE = 301 + RSIZE * ICREC + GNEXTN = GETRS(IDEVEN,BARRAY,RSIZE,IBYTE) + IF(GNEXTN.NE.0) RETURN + CALL BSW4(BARRAY(11:),ANNOFF) + CALL BSW4(BARRAY(15:),SEQOFF) + CALL BSW2(BARRAY(19:),STAR2) + DIVCOD = STAR2 + ENAME = BARRAY(1:10) + ICREC = ICREC + 1 + END + INTEGER FUNCTION ENTFN(IDEV,DIVCOD,LIBF,BARRAY) + CHARACTER LIBF*(*),BARRAY*(*) + INTEGER DIVCOD + ENFTN = 1 + REWIND IDEV + 10 CONTINUE + READ(IDEV,1000,ERR=100,END=200)I,BARRAY + 1000 FORMAT(I6,1X,A) + IF(I.NE.DIVCOD) GO TO 10 + LIBF = BARRAY + ENTFN = 0 + RETURN + 100 CONTINUE + ENTFN = -2 + RETURN + 200 CONTINUE + ENTFN = -1 + END + INTEGER FUNCTION OPENFU(IDEV,NAME) + CHARACTER NAME*(*) + CLOSE(UNIT=IDEV) + LRECL = 1 + CALL OPENRS(IDEV,NAME,IOK,LRECL,11) + OPENFU = 0 + IF(IOK.EQ.0) RETURN + OPENFU = 1 + END + INTEGER FUNCTION OPENFF(IDEV,NAME) + CHARACTER NAME*(*) + CLOSE(UNIT=IDEV) + CALL OPENRS(IDEV,NAME,IOK,LRECL,2) + OPENFF = 0 + IF(IOK.EQ.0) RETURN + OPENFF = 1 + END + INTEGER FUNCTION IHEAD(IDEVEN,BARRAY,NREC,RSIZE) + INTEGER NREC,RSIZE,GETRS + INTEGER*2 IRSIZE + CHARACTER BARRAY*(*) + EXTERNAL GETRS + IHEAD = GETRS(IDEVEN,BARRAY,10,1) + IF(IHEAD.NE.0) RETURN +C CALL BSW4(BARRAY(1:),FSIZE) + CALL BSW4(BARRAY(5:),NREC) + CALL BSW2(BARRAY(9:),IRSIZE) + RSIZE = IRSIZE + END +C below are routines for handling the embl and genbank feature tables +C Not all cases are dealt with, but those for automatic translation to +C protein are done for all the straightforward cases I can think of. +C Their robustness has yet to be tested! + INTEGER FUNCTION EMBLFT(IDEV,KBOUT,KEYWRD,OPRATR,ISTRAN, + +POSNS,MAXPOS,NPOS,NOBJ) +C interpret ft looking for keyword, operator and strand +C return list of end points, number of positions and +C number of objects + CHARACTER LINEIN*80,KEYWRD*(*),OPRATR*(*) + INTEGER POSNS(MAXPOS) + INTEGER FTIN1,FTIN4,FTIN5,FTIN6,FTIN7,FTIN8 + INTEGER FTIN9,FTIN10,FTIN11,RDFTLN + LOGICAL NUMBER,STRNGM,STRNGN,WSTRND + EXTERNAL FTIN1,FTIN4,FTIN5,FTIN6,FTIN7,FTIN8 + EXTERNAL FTIN9,FTIN10,FTIN11,STRNGM,STRNGN,WSTRND,RDFTLN + NPOS = 0 + NPOS1 = 0 +C Length of opratr + LENOP = INDEX(OPRATR,' ') - 1 +C Find start +10 CONTINUE + EMBLFT = RDFTLN(IDEV,LINEIN) + IF(EMBLFT.LT.-1) RETURN + IF(EMBLFT.NE.-1) GO TO 10 +C For embl allow extra FH line + IF(LINEIN(1:2).EQ.'FH') EMBLFT = RDFTLN(IDEV,LINEIN) + IF(EMBLFT.NE.-1) RETURN +C Into ft +20 CONTINUE + IERR = RDFTLN(IDEV,LINEIN) +C End of ft ? + IF(IERR.EQ.-2) GO TO 400 +C Error ? + IF(IERR.NE.0) RETURN +25 CONTINUE + IF(.NOT.STRNGM(LINEIN(6:),KEYWRD)) GO TO 20 + IF(LENOP.GT.0) THEN + CALL CCASE(LINEIN,1) + J = INDEX(LINEIN,OPRATR(1:LENOP)) + IF(J.EQ.0) GO TO 20 + END IF +C IF(.NOT.STRNGN(LINEIN(22:),OPRATR)) GO TO 20 + IF(.NOT.WSTRND(LINEIN,ISTRAN)) GO TO 20 +C +C Only get here if keyword, operator and strand are correct +C + IF(NUMBER(LINEIN(22:22))) THEN + EMBLFT = FTIN1(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + ELSE IF(LINEIN(22:22).EQ.'<') THEN + EMBLFT = FTIN1(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + ELSE IF(LINEIN(22:22).EQ.'>') THEN + EMBLFT = FTIN1(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + ELSE IF(LINEIN(22:22).EQ.'"') THEN + WRITE(KBOUT,*)'Not handled' + EMBLFT = FTIN4(IDEV,POSNS,MAXPOS,NPOS,LINEIN) +C +C note order of elses important +C + ELSE IF(STRNGM(LINEIN(22:37),'JOIN(COMPLEMENT(')) THEN + EMBLFT = FTIN8(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + ELSE IF(STRNGM(LINEIN(22:26),'JOIN(')) THEN + EMBLFT = FTIN5(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + ELSE IF(STRNGM(LINEIN(22:38),'ORDER(COMPLEMENT(')) THEN + EMBLFT = FTIN10(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + ELSE IF(STRNGM(LINEIN(22:27),'ORDER(')) THEN + EMBLFT = FTIN9(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + ELSE IF(STRNGM(LINEIN(22:38),'COMPLEMENT(ORDER(')) THEN + EMBLFT = FTIN11(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + ELSE IF(STRNGM(LINEIN(22:32),'COMPLEMENT(')) THEN + EMBLFT = FTIN6(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + ELSE IF(LINEIN(22:22).EQ.'/') THEN + WRITE(KBOUT,*)'Not handled' + EMBLFT = FTIN7(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + ELSE + WRITE(KBOUT,*)'line of unknown type' + EMBLFT = 9 + END IF + IF(EMBLFT.NE.0) THEN +C +C -1 = start of ft +C -2 = end of entry or file +C -3 = error reading file +C -4 = error getting numbers from a line +C >0 = error in ftinN where error=N +C -9 = overflow of storage space +C + WRITE(KBOUT,*)'Error no',EMBLFT + WRITE(KBOUT,*)LINEIN + RETURN + END IF + IF(NPOS.GT.NPOS1) THEN + IF(NPOS+1.GT.MAXPOS) THEN + WRITE(KBOUT,*)'Number of endpoints and objects exceeds',MAXPOS + EMBLFT = -9 + RETURN + END IF + POSNS(NPOS+1) = NPOS - NPOS1 + NPOS = NPOS + 1 + NPOS1 = NPOS + NOBJ = NOBJ + 1 + END IF + GO TO 25 +400 CONTINUE + EMBLFT = 0 + WRITE(KBOUT,*)'End of entry' + END + LOGICAL FUNCTION WSTRND(LINEIN,WANTED) +C Return true if line is for wanted strand +C Assume complementary strands will contain string "complement" +C and that other strand wont +C Plus strand wanted = 0, minus strand wanted = 1 +C (also works if wanted =2, meaning either strand) + CHARACTER LINEIN*(*) + INTEGER WANTED + WSTRND = .FALSE. + CALL CCASE(LINEIN,1) + I = INDEX(LINEIN,'COMPLEMENT') + IF((I.EQ.0).AND.(WANTED.EQ.1)) RETURN + IF((I.NE.0).AND.(WANTED.EQ.0)) RETURN + WSTRND = .TRUE. + END + LOGICAL FUNCTION STRNGN(S1,S2) +C return true if the first L chars of s1 and s2 match +C L is the length of the shortest string or the position of +C the first space char -1. Note if one is only spaces they will match. + CHARACTER S1*(*),S2*(*),TUPPER + EXTERNAL TUPPER + STRNGN = .FALSE. + L = MIN(LEN(S1),LEN(S2)) + I = MIN(INDEX(S1,' '),INDEX(S2,' ')) + L = MIN(L,MAX(0,I-1)) + DO 10 I = 1,L + IF(TUPPER(S1(I:I)).NE.(TUPPER(S2(I:I)))) RETURN + 10 CONTINUE + STRNGN = .TRUE. + END + INTEGER FUNCTION RDFTLN(IDEV,LINEIN) + CHARACTER LINEIN*(*) +C +C -1 = start of ft +C -2 = end of entry or file +C -3 = error reading file +C +C read ft lines + READ(IDEV,1000,END=200,ERR=300)LINEIN +C check for start of data + IF((LINEIN(1:2).EQ.'FH').OR.(LINEIN(1:8).EQ.'FEATURES')) THEN + RDFTLN = -1 + RETURN + END IF +C check for end of data + IF((LINEIN(1:2).EQ.'SQ').OR.(LINEIN(1:2).EQ.'//')) GO TO 200 +C WRITE(*,*)LINEIN + RDFTLN = 0 + RETURN +1000 FORMAT(A) +200 CONTINUE + RDFTLN = -2 + RETURN +300 CONTINUE + RDFTLN = -3 + END + INTEGER FUNCTION FTIN1(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + INTEGER POSNS(MAXPOS) + CHARACTER LINEIN*(*) + INTEGER FTPAIR,RDFTLN + EXTERNAL FTPAIR,RDFTLN +C 467 +C 340..565 +C 102.110 +C 123^124 +C 145^177 +C find start n1, end n2 of first number +C find start n3, end n3 of second number +C decode into posns + FTIN1 = 1 + IF(NPOS+2.GT.MAXPOS) RETURN + N1 = 22 + FTIN1 = FTPAIR(LINEIN,N1,J1,J2) + IF(FTIN1.NE.0) RETURN + POSNS(NPOS+1) = J1 + POSNS(NPOS+2) = J2 + NPOS = NPOS + 2 + FTIN1 = RDFTLN(IDEV,LINEIN) + END + INTEGER FUNCTION FTIN6(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + INTEGER POSNS(MAXPOS) + CHARACTER LINEIN*(*) + INTEGER FTPAIR,RDFTLN + LOGICAL NUMBER + EXTERNAL FTPAIR,NUMBER,RDFTLN +C only deal with simplest case: +C complement(123..345) + FTIN6 = 6 + N1 = 33 +10 CONTINUE + IF(NPOS+2.GT.MAXPOS) RETURN + FTIN6 = FTPAIR(LINEIN,N1,J1,J2) + IF(FTIN6.NE.0) THEN + IERR = RDFTLN(IDEV,LINEIN) + RETURN + END IF + POSNS(NPOS+1) = J1 + POSNS(NPOS+2) = J2 + NPOS = NPOS + 2 +C allow next position to be ')' + IF(LINEIN(N1+1:N1+1).EQ.')') THEN + FTIN6 = RDFTLN(IDEV,LINEIN) + RETURN + ELSE + IERR = RDFTLN(IDEV,LINEIN) + FTIN6 = 6 + END IF + FTIN6 = 6 + END + INTEGER FUNCTION FTIN5(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + INTEGER POSNS(MAXPOS) + CHARACTER LINEIN*(*) + INTEGER FTPAIR,RDFTLN + LOGICAL NUMBER + EXTERNAL FTPAIR,NUMBER,RDFTLN +C only deal with simplest cases: +C join(123..345,456..666) +C join(123..345,456..666, +C 789..899) +C assume continues lines end with ", " +C always terminate at first closing bracket + FTIN5 = 5 + N1 = 27 +10 CONTINUE + IF(NPOS+2.GT.MAXPOS) RETURN + FTIN5 = FTPAIR(LINEIN,N1,J1,J2) + IF(FTIN5.NE.0) THEN + IERR = RDFTLN(IDEV,LINEIN) + RETURN + END IF + POSNS(NPOS+1) = J1 + POSNS(NPOS+2) = J2 + NPOS = NPOS + 2 +C allow next position to be ',' or ')' + IF(LINEIN(N1+1:N1+1).EQ.')') THEN + FTIN5 = RDFTLN(IDEV,LINEIN) + RETURN + ELSE IF(LINEIN(N1+1:N1+1).EQ.',') THEN + IF(NUMBER(LINEIN(N1+2:N1+2))) THEN + N1 = N1 + 2 +C if ,number + GO TO 10 + ELSE + IF(LINEIN(N1+2:N1+2).NE.' ') THEN + IERR = RDFTLN(IDEV,LINEIN) +C if ,notspace + FTIN5 = 5 + RETURN + END IF + END IF + END IF + IERR = RDFTLN(IDEV,LINEIN) + IF(IERR.NE.0) THEN + FTIN5 = IERR + RETURN + END IF + IF(LINEIN(6:8).EQ.' ') THEN + N1 = 22 + GO TO 10 + END IF + FTIN5 = 5 + END + INTEGER FUNCTION FTIN8(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + INTEGER POSNS(MAXPOS) + CHARACTER LINEIN*(*) + INTEGER FTPAIR,RDFTLN + LOGICAL NUMBER,STRNGM + EXTERNAL FTPAIR,NUMBER,STRNGM,RDFTLN +C only deal with simplest cases: +C join(complement(123..345),complement(59..67)) +C join(complement(123..345),complement(59..67), +C complement(44..55)) +C assume continues lines end with ", " +C always terminate at first closing bracket +C remember start + NPOS1 = NPOS + FTIN8 = 8 + N1 = 38 +10 CONTINUE + IF(NPOS+2.GT.MAXPOS) RETURN + FTIN8 = FTPAIR(LINEIN,N1,J1,J2) + IF(FTIN8.NE.0) THEN + IERR = RDFTLN(IDEV,LINEIN) + END IF + POSNS(NPOS+1) = J1 + POSNS(NPOS+2) = J2 + NPOS = NPOS + 2 +C allow next position to be '),COMPLEMENT' or ')) ' + IF(LINEIN(N1+1:N1+2).EQ.'))') THEN + FTIN8 = RDFTLN(IDEV,LINEIN) + CALL REVFTP(POSNS(NPOS1+1),NPOS-NPOS1) + RETURN + ELSE IF(STRNGM(LINEIN(N1+1:N1+12),'),COMPLEMENT')) THEN + N1 = N1 + 14 +C if ,number + GO TO 10 + ELSE + IF(LINEIN(N1+1:N1+3).NE.'), ') THEN + IERR = RDFTLN(IDEV,LINEIN) +C if ,notspace + FTIN8 = 8 + RETURN +C END IF + END IF + END IF + IERR = RDFTLN(IDEV,LINEIN) + IF(IERR.NE.0) THEN + FTIN8 = IERR + RETURN + END IF + IF(LINEIN(6:8).EQ.' ') THEN + N1 = 33 + GO TO 10 + END IF + FTIN8 = 8 + END + SUBROUTINE REVFTP(POSNS,NPOS) +C Reverses posns for join(complement()) and order(complement()) + INTEGER POSNS(NPOS) + DO 10 I = 1,NPOS-1,2 + J = POSNS(I) + POSNS(I) = POSNS(I+1) + POSNS(I+1) = J + 10 CONTINUE + DO 20 I = 1,NPOS/2 + J = POSNS(I) + POSNS(I) = POSNS(NPOS-I+1) + POSNS(NPOS-I+1) = J + 20 CONTINUE + END + INTEGER FUNCTION FTIN9(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + INTEGER POSNS(MAXPOS) + CHARACTER LINEIN*(*) + INTEGER FTPAIR,RDFTLN + LOGICAL NUMBER + EXTERNAL FTPAIR,NUMBER,RDFTLN +C only deal with simplest cases: +C order(123..345,456..666) +C order(123..345,456..666, +C 789..899) +C assume continues lines end with ", " +C always terminate at first closing bracket + FTIN9 = 9 + N1 = 28 +10 CONTINUE + IF(NPOS+2.GT.MAXPOS) RETURN + FTIN9 = FTPAIR(LINEIN,N1,J1,J2) + IF(FTIN9.NE.0) THEN + IERR = RDFTLN(IDEV,LINEIN) + RETURN + END IF + POSNS(NPOS+1) = J1 + POSNS(NPOS+2) = J2 + NPOS = NPOS + 2 +C allow next position to be ',' or ')' + IF(LINEIN(N1+1:N1+1).EQ.')') THEN + FTIN9 = RDFTLN(IDEV,LINEIN) + RETURN + ELSE IF(LINEIN(N1+1:N1+1).EQ.',') THEN + IF(NUMBER(LINEIN(N1+2:N1+2))) THEN + N1 = N1 + 2 +C if ,number + GO TO 10 + ELSE + IF(LINEIN(N1+2:N1+2).NE.' ') THEN + IERR = RDFTLN(IDEV,LINEIN) +C if ,notspace + FTIN9 = 9 + RETURN + END IF + END IF + END IF + IERR = RDFTLN(IDEV,LINEIN) + IF(IERR.NE.0) THEN + FTIN9 = IERR + RETURN + END IF + IF(LINEIN(6:8).EQ.' ') THEN + N1 = 22 + GO TO 10 + END IF + FTIN9 = 9 + END + INTEGER FUNCTION FTIN10(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + INTEGER POSNS(MAXPOS) + CHARACTER LINEIN*(*) + INTEGER FTPAIR,RDFTLN + LOGICAL NUMBER,STRNGM + EXTERNAL FTPAIR,NUMBER,STRNGM,RDFTLN +C only deal with simplest cases: +C order(complement(123..345),complement(59..67)) +C order(complement(123..345),complement(59..67), +C complement(44..55)) +C assume continues lines end with ", " +C always terminate at first closing bracket +C remember start + NPOS1 = NPOS + FTIN10 = 10 + N1 = 39 +10 CONTINUE + IF(NPOS+2.GT.MAXPOS) RETURN + FTIN10 = FTPAIR(LINEIN,N1,J1,J2) + IF(FTIN10.NE.0) THEN + IERR = RDFTLN(IDEV,LINEIN) + RETURN + END IF + POSNS(NPOS+1) = J1 + POSNS(NPOS+2) = J2 + NPOS = NPOS + 2 +C allow next position to be '),COMPLEMENT' or ')) ' + IF(LINEIN(N1+1:N1+2).EQ.'))') THEN + FTIN10 = RDFTLN(IDEV,LINEIN) + CALL REVFTP(POSNS(NPOS1+1),NPOS-NPOS1) + RETURN + ELSE IF(STRNGM(LINEIN(N1+1:N1+12),'),COMPLEMENT')) THEN + N1 = N1 + 13 +C if ,number + GO TO 10 + ELSE + IF(LINEIN(N1+1:N1+3).NE.'), ') THEN + IERR = RDFTLN(IDEV,LINEIN) +C if ,notspace + FTIN10 = 10 + RETURN +C END IF + END IF + END IF + IERR = RDFTLN(IDEV,LINEIN) + IF(IERR.NE.0) THEN + FTIN10 = IERR + RETURN + END IF + IF(LINEIN(6:8).EQ.' ') THEN + N1 = 33 + GO TO 10 + END IF + FTIN10 = 10 + END + INTEGER FUNCTION FTIN11(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + INTEGER POSNS(MAXPOS) + CHARACTER LINEIN*(*) + INTEGER FTPAIR,RDFTLN + LOGICAL NUMBER,STRNGM + EXTERNAL FTPAIR,NUMBER,STRNGM,RDFTLN +C only deal with simplest cases: +C complement(order(123..345,456..666)) +C complement(order(123..345,456..666, +C 789..899)) +C assume continues lines end with ", " +C always terminate at first closing bracket + FTIN11 = 11 + N1 = 39 +10 CONTINUE + IF(NPOS+2.GT.MAXPOS) RETURN + FTIN11 = FTPAIR(LINEIN,N1,J1,J2) + IF(FTIN11.NE.0) THEN + IERR = RDFTLN(IDEV,LINEIN) + RETURN + END IF + POSNS(NPOS+1) = J1 + POSNS(NPOS+2) = J2 + NPOS = NPOS + 2 +C allow next position to be ',' or ')) ' + IF(LINEIN(N1+1:N1+2).EQ.'))') THEN + FTIN11 = RDFTLN(IDEV,LINEIN) + RETURN + ELSE IF(LINEIN(N1+1:N1+1).EQ.',') THEN + IF(NUMBER(LINEIN(N1+2:N1+2))) THEN + N1 = N1 + 2 +C if ,number + GO TO 10 + ELSE + IF(LINEIN(N1+2:N1+2).NE.' ') THEN + IERR = RDFTLN(IDEV,LINEIN) +C if ,notspace + FTIN11 = 11 + RETURN + END IF + END IF + END IF + IERR = RDFTLN(IDEV,LINEIN) + IF(IERR.NE.0) THEN + FTIN11 = IERR + RETURN + END IF + IF(LINEIN(6:8).EQ.' ') THEN + N1 = 22 + GO TO 10 + END IF + FTIN11 = 11 + END + INTEGER FUNCTION FTPAIR(LINEIN,N1,J1,J2) + CHARACTER LINEIN*(*) + INTEGER FIRSTN + EXTERNAL LASTN,FIRSTN + FTPAIR = -4 +C find first and last digits in each of two numbers (n1,n2 and n3,n4) +C then encode them + J = INDEX(LINEIN(N1:),'.') +C Could be cases 1,4,5 but for now error + IF(J.EQ.0) RETURN + J = FIRSTN(LINEIN(N1:),1) + IF(J.EQ.0) RETURN + N1 = N1 + J - 1 + J = INDEX(LINEIN(N1:),'.') + N2 = N1 + J - 2 + K = N2 + 2 + J = INDEX(LINEIN(K:),'.') + IF(J.EQ.0) RETURN + IF(J.NE.1) RETURN + N3 = J + K + J = FIRSTN(LINEIN(N3:),1) + IF(J.EQ.0) RETURN + N3 = N3 + J - 1 + K = N3 + J = LASTN(LINEIN,K) + IF(J.EQ.0) RETURN + N4 = J + READ(LINEIN(N1:N2),1000,ERR=100)J1 +1000 FORMAT(I7) + READ(LINEIN(N3:N4),1000,ERR=100)J2 + FTPAIR = 0 + N1 = N4 + RETURN +100 CONTINUE +C WRITE(*,*)'Scream: ftpair' + END + INTEGER FUNCTION FTIN7(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + INTEGER POSNS(MAXPOS),RDFTLN + CHARACTER LINEIN*(*) + EXTERNAL RDFTLN + FTIN7 = RDFTLN(IDEV,LINEIN) + END + INTEGER FUNCTION FTIN4(IDEV,POSNS,MAXPOS,NPOS,LINEIN) + INTEGER POSNS(MAXPOS),RDFTLN + CHARACTER LINEIN*(*) + EXTERNAL RDFTLN + FTIN4 = RDFTLN(IDEV,LINEIN) + END diff --git a/src/staden/sethelp.c b/src/staden/sethelp.c new file mode 100644 index 0000000..d1a9482 --- /dev/null +++ b/src/staden/sethelp.c @@ -0,0 +1,88 @@ +/* + * sethelp: Generates the help index and menu data file. + */ + +#include +#include +#include +#include +#include + +void format_file(FILE *hp, FILE *mp); + +int main(int argc, char *argv[]) { + char helpfile[MAXPATHLEN], menufile[MAXPATHLEN]; + FILE *hp, *mp; + int i, err = 0; + + if (argc < 2) { + fprintf(stderr, "Usage:%s program ...\n", *argv); + return 1; + } + + for (i=1; i L + M = 0 + MTOT = 0 + FRONT = SPAN + BACK = 0 + DO 10 I = 1,MIN(SPAN,L) + M = M + MATRIX(SEQ1(I),SEQ2(I)) +10 CONTINUE + IF(M.GT.MINSCR) MTOT = M + DO 20 I = 2,L-SPAN+1 + FRONT = FRONT + 1 + BACK = BACK + 1 + MM = MATRIX(SEQ1(BACK),SEQ2(BACK)) + MP = MATRIX(SEQ1(FRONT),SEQ2(FRONT)) + M = M - MM + MP + IF(M.GT.MINSCR) MTOT = MTOT + M +20 CONTINUE + ESCORE = MTOT + END diff --git a/src/staden/sipmen.f b/src/staden/sipmen.f new file mode 100644 index 0000000..913f090 --- /dev/null +++ b/src/staden/sipmen.f @@ -0,0 +1,84 @@ + SUBROUTINE MENU(OPT,KOPT,MOPT,MAXOPT,MINMEN,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + INTEGER OPT + INTEGER IHELPS(0:MAXOPT),IHELPE(0:MAXOPT) + CHARACTER HELPF*(*) +1 CONTINUE + CALL CLEARV + IF(MOPT.EQ.0)THEN + WRITE(KBOUT,5000) +5000 FORMAT(' Menus and their numbers are'/ + +' m0 = This menu'/ + 1' m1 = General'/ + 2' m2 = Screen control'/ + 5' m3 = Set parameters'/ + +' m4 = Comparison'/ + 6' ? = Help'/ + 1' ! = Quit') +1001 FORMAT(' ? Menu or option number=',$) + ELSE IF(MOPT.EQ.-1)THEN + WRITE(KBOUT,5001) +5001 FORMAT(' General menu'/ + + ' 0 = List of menus'/ + 1 ' 3 = Read new sequence'/ + 1 ' 4 = Redefine active region'/ + + ' 5 = List the sequences'/ + + ' 6 = List a text file'/ + + ' 7 = Direct output to disk'/ + + ' 8 = Write active sequence to disk'/ + + ' 9 = Edit the sequences'/ + + ' 29 = Complement sequences') + ELSE IF(MOPT.EQ.-2)THEN + WRITE(KBOUT,5002) +5002 FORMAT(' Screen control menu'/ + + ' 0 = List of menus'/ + + ' 10 = Clear graphics'/ + + ' 11 = Clear text'/ + + ' 12 = Draw a ruler'/ + + ' 13 = Use cross hair'/ + + ' 14 = Reposition plots'/ + + ' 15 = Label diagram'/ + + ' 16 = Display a map'/ + + ' 27 = Draw a /') + ELSE IF(MOPT.EQ.-3)THEN + WRITE(KBOUT,5005) +5005 FORMAT(' Set parameters menu'/ + + ' 0 = List of menus'/ + + ' 20 = Set span length'/ + + ' 21 = Set proportional score'/ + + ' 22 = Set identites score'/ + + ' 23 = Calculate expected scores'/ + + ' 24 = Calculate observed scores'/ + + ' 25 = Show current parameter settings'/ + + ' 30 = Switch main diagonal'/ + + ' 31 = Switch identities'/ + + ' 32 = Change score matrix'/ + + ' 33 = Set number of sd for Quickscan'/ + + ' 34 = Set gap penalties') + ELSE IF(MOPT.EQ.-4)THEN + WRITE(KBOUT,5006) +5006 FORMAT(' Comparison menu'/ + + ' 0 = List of menus'/ + + ' 17 = Apply identities algorithm'/ + + ' 18 = Apply proportional algorithm'/ + + ' 19 = List matching spans'/ + + ' 26 = Quick scan'/ + + ' 28 = Align sequences') + END IF +3 CONTINUE + WRITE(KBOUT,1001) + CALL GETOPT(KBIN,KOPT,OPT) + IF((OPT.LT.MINMEN).OR.(OPT.GT.MAXOPT).OR.(KOPT.GT.2)) THEN + MOPT = 0 + GO TO 1 + END IF + IF((KOPT.EQ.2).AND.(OPT.GE.0)) THEN + CALL HELP2(IHELPS(OPT),IHELPE(OPT),HELPF,IDEVH,KBIN,KBOUT) + GO TO 1 + END IF + IF(OPT.LE.0) THEN + MOPT = OPT + GO TO 1 + END IF + END diff --git a/src/staden/splitp1.f b/src/staden/splitp1.f new file mode 100644 index 0000000..3f7efe3 --- /dev/null +++ b/src/staden/splitp1.f @@ -0,0 +1,58 @@ +C ROUTINE TO TRANSLATE PROSITE DATABASE +C 2-3-92 set filnam = ' ' + SUBROUTINE FMAIN() + PARAMETER (MAXSTR = 80, MAXDEV = 4) + INTEGER DEVNOS(MAXDEV) + CHARACTER*(MAXSTR) AMOS,IDLINE,TITLE + CHARACTER*40 PATNAM,HELPF,FILNAM + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + WRITE(KBOUT,*)'SPLITP1 splits prosite.dat into one file per entry' + WRITE(KBOUT,*)' and creates and index' + IDEV1 = DEVNOS(1) + IDEV2 = DEVNOS(2) + IDEV4 = DEVNOS(4) + FILNAM = ' ' + CALL OPENF1(DEVNOS(1),FILNAM,0,IOK,KBIN,KBOUT, + +'Prosite library file', + +IHELPS,IHELPE,HELPF,DEVNOS(3)) + IF(IOK.NE.0) STOP + FILNAM = ' ' + CALL OPENF1(DEVNOS(4),FILNAM,1,IOK,KBIN,KBOUT, + +'Index file', + +IHELPS,IHELPE,HELPF,DEVNOS(3)) + IF(IOK.NE.0) STOP + IPAT = 0 +5 CONTINUE + READ(IDEV1,1000,ERR=200,END=100)AMOS +1000 FORMAT(A) + IF(AMOS(1:2).EQ.'ID') THEN + IDLINE = AMOS + GO TO 5 + END IF + IF(AMOS(1:2).EQ.'AC') THEN + IPAT = IPAT + 1 + NAMLEN = INDEX(AMOS(1:),';') - 1 + PATNAM = AMOS(6:NAMLEN)//'.DAT' +1005 FORMAT(' ',A) + CALL OPENRS(IDEV2,PATNAM,IOK,LRECL,1) + IF(IOK.NE.0) WRITE(*,*)'SCREAM, FILE OPENING' +1009 FORMAT(' ',A) + WRITE(IDEV2,1009,ERR=200)IDLINE + WRITE(IDEV2,1009,ERR=200)AMOS + END IF +6 CONTINUE + READ(IDEV1,1000,ERR=200,END=100)AMOS + WRITE(IDEV2,1009,ERR=200)AMOS + IF(AMOS(1:2).EQ.'DE') TITLE = AMOS(6:) + IF(AMOS(1:2).EQ.'DO') THEN + TITLE(70:80) = PATNAM(3:7)//','//AMOS(10:14) + WRITE(IDEV4,1009,ERR=200)TITLE + END IF + IF(AMOS(1:2).NE.'//') GO TO 6 + GO TO 5 +100 CONTINUE + WRITE(KBOUT,*)IPAT,' files created. Normal termination' + STOP +200 CONTINUE + WRITE(KBOUT,*)IPAT,' files created. Abnormal termination' + END diff --git a/src/staden/splitp2.f b/src/staden/splitp2.f new file mode 100644 index 0000000..46c0e0d --- /dev/null +++ b/src/staden/splitp2.f @@ -0,0 +1,40 @@ +C ROUTINE TO TRANSLATE PROSITE DATABASE + SUBROUTINE FMAIN() + PARAMETER (MAXSTR = 80, MAXDEV = 3) + INTEGER DEVNOS(MAXDEV) + CHARACTER AMOS*(MAXSTR) + CHARACTER*40 PATNAM,HELPF,FILNAM + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + WRITE(KBOUT,*)'SPLITP2 splits prosite.doc into one file per entry' + IDEV1 = DEVNOS(1) + IDEV2 = DEVNOS(2) + FILNAM = ' ' + CALL OPENF1(DEVNOS(1),FILNAM,0,IOK,KBIN,KBOUT, + +'Prosite library file', + +IHELPS,IHELPE,HELPF,DEVNOS(3)) + IF(IOK.NE.0) STOP + IPAT = 0 +5 CONTINUE + READ(IDEV1,1000,ERR=200,END=100)AMOS +1000 FORMAT(A) + IF(AMOS(1:5).EQ.'{PDOC') THEN + IPAT = IPAT + 1 + NAMLEN = INDEX(AMOS(1:),'}') - 1 + PATNAM = AMOS(2:NAMLEN)//'.DOC' +1005 FORMAT(' ',A) + CALL OPENRS(IDEV2,PATNAM,IOK,LRECL,1) + IF(IOK.NE.0) WRITE(*,*)'SCREAM, FILE OPENING' +1009 FORMAT(' ',A) + WRITE(IDEV2,1009,ERR=200)AMOS + END IF +6 CONTINUE + READ(IDEV1,1000,ERR=200,END=100)AMOS + WRITE(IDEV2,1009,ERR=200)AMOS + IF(AMOS(1:4).NE.'{END') GO TO 6 + GO TO 5 +100 CONTINUE + WRITE(KBOUT,*)IPAT,' files created. Normal termination' + STOP +200 CONTINUE + WRITE(KBOUT,*)IPAT,' files created. Abnormal termination' + END diff --git a/src/staden/splitp3.f b/src/staden/splitp3.f new file mode 100644 index 0000000..616428f --- /dev/null +++ b/src/staden/splitp3.f @@ -0,0 +1,392 @@ +C Routine to translate prosite motif library into patterns +C assumes only exact match, membership of set and NOT +C assumes no errors in library +C assumes gaps up to 40 +C assumes no variation in length of motif: only variation in +C their separation ie [fred](2,3) is not dealt with (but why should it exist) +C but x(2,3) causes a new motif to be started starting 2 away with 1 extra +C position. +C the translation is crude: all go to membership of set +C nots go to membership of set for the rest of the character set +C fixed gaps are included in "weight matrices" and so will slow searches +C 18-7-91 Added titles to pattern files +C 3-2-92 Changed program so it writes pattern and weight files +C to current directory. All file names in the fofn and +C pattern files are full path names. + SUBROUTINE FMAIN() + INTEGER AP,RP,SCORE + PARAMETER (MAXSTR = 255,MAXLEN = 120, IDM = 26, MAXDEV = 5) + INTEGER WTSMAT(IDM,MAXLEN) + INTEGER DEVNOS(MAXDEV) + CHARACTER AMOS*(MAXSTR),RS*(MAXSTR),CACIDS*20,COMMAS*40 + CHARACTER AAMOS*(MAXSTR) + CHARACTER*60 PATNAM,WTSNAM,HELPF,FILNAM + CHARACTER CSET*26,TITLE*80 + LOGICAL ACID,NUMBER + EQUIVALENCE (AAMOS(6:),AMOS) + EXTERNAL ACID,NUMBER,INTFC + DATA COMMAS/',,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,'/ + DATA CSET/'ABCDEFGHIJKLMNOPQRSTUVWXYZ'/ + CALL INITLU(IDM) + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + WRITE(KBOUT,*) + +' SPLITP3 v3.0 Feb 1992, author Rodger Staden' + WRITE(KBOUT,*) + +' Splits Prosite motif library into Staden pattern files' + WRITE(KBOUT,*) + +' and creates a file of file names. The pattern files and' + WRITE(KBOUT,*) + +' weight matrices are written to the current directory but' + WRITE(KBOUT,*) + +' full path names are included in the file of file names' + IDEV1 = DEVNOS(1) + IDEV2 = DEVNOS(2) + IDEV3 = DEVNOS(3) + IDEV4 = DEVNOS(4) + NVAR = 0 + NMOTIF = 0 + FILNAM = ' ' + CALL OPENF1(DEVNOS(1),FILNAM,0,IOK,KBIN,KBOUT, + +'Prosite library file', + +IHELPS,IHELPE,HELPF,DEVNOS(5)) + IF(IOK.NE.0) STOP + FILNAM = ' ' + CALL OPENF1(DEVNOS(2),FILNAM,1,IOK,KBIN,KBOUT, + +'Name for file of pattern file names', + +IHELPS,IHELPE,HELPF,DEVNOS(5)) + IF(IOK.NE.0) STOP +3 CONTINUE + LIN = 0 + CALL GTSTR('Path name of motif directory',' ',PATNAM,LIN, + +KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,DEVNOS(5),KBIN,KBOUT) + GO TO 3 + END IF + IF(INFLAG.EQ.2) STOP + IDIR = MAX(1,INDEX(PATNAM,' ')) +C +C add final slash if not present + IF(PATNAM(IDIR-1:IDIR-1).NE.'/') THEN + PATNAM(IDIR:IDIR) = '/' + IDIR = IDIR + 1 + END IF +5 CONTINUE + READ(IDEV1,1000,ERR=100,END=200)AAMOS +1000 FORMAT(A) + IF(AAMOS(1:2).NE.'AC') GO TO 5 + NAMLEN = INDEX(AMOS(1:),';') - 1 + PATNAM(IDIR:) = AMOS(1:NAMLEN) + WTSNAM = PATNAM + PATNAM = PATNAM(1:NAMLEN+IDIR-1)//'.PAT' + CALL OPENRS(IDEV3,PATNAM(IDIR:),IOK,LRECL,1) + IF(IOK.NE.0) WRITE(*,*)'SCREAM, FILE OPENING' +6 CONTINUE + READ(IDEV1,1000,ERR=100,END=200)AAMOS + IF(AAMOS(1:2).NE.'DE') GO TO 6 + TITLE = AAMOS(6:) + WRITE(IDEV3,1002)TITLE +9 CONTINUE + READ(IDEV1,1000,ERR=100,END=200)AAMOS +C Check for empty definition ie no pa line + IF(AAMOS(1:2).EQ.'//') THEN + WRITE(KBOUT,*)TITLE + WRITE(KBOUT,*)'No PA line!' + CLOSE(UNIT=IDEV3) + GO TO 5 + END IF + IF(AAMOS(1:2).NE.'PA') GO TO 9 + IF(INDEX(AMOS,'.').EQ.0) THEN + J = INDEX(AMOS,' ') + READ(IDEV1,1001,ERR=100,END=200)AMOS(J:) +1001 FORMAT(5X,A) + END IF + LAMOS = INDEX(AMOS,'.') +C WRITE(KBOUT,*)AMOS(1:LAMOS) + NMOTIF = NMOTIF + 1 + AP = 0 + RP = 0 + SCORE = 0 + MOTIF = 1 + WRITE(IDEV3,1002)'A6 '// + +PATNAM(IDIR+2:IDIR+6)//' Class' +1002 FORMAT(A) +10 CONTINUE + AP = AP + 1 + IF(AP.LT.LAMOS) THEN + IF(AMOS(AP:AP).EQ.'-') AP = AP + 1 + IF(ACID(AMOS(AP:))) THEN + RP = RP + 1 + RS(RP:RP) = AMOS(AP:AP) + LASTS = RP + RP = RP + 1 + RS(RP:RP) = ',' + SCORE = SCORE + 1 + ELSE IF(AMOS(AP:AP).EQ.'[') THEN + AP = AP + 1 + LSET = INDEX(AMOS(AP:),']') - 1 + RP = RP + 1 + RS(RP:RP+LSET-1) = AMOS(AP:AP+LSET-1) + LASTS = RP + RP = RP + LSET + AP = AP + LSET + RS(RP:RP) = ',' + AP = AP + 1 + SCORE = SCORE + 1 + IF(AMOS(AP:AP).EQ.'(') THEN + AP = AP + 1 + LSET = INDEX(AMOS(AP:),')') - 1 + ICOMMA = INDEX(AMOS(AP:),',') + IF((ICOMMA.GT.0).AND.(ICOMMA.LT.LSET)) THEN + WRITE(KBOUT,*)'Variable gap in []' + NVAR = NVAR + 1 + GO TO 5 + END IF + NREP = INTFC(AMOS(AP:),LSET) + LREP = RP - LASTS + RP = RP + 1 + CALL REPST(RS(LASTS:),RS(RP:),LREP,NREP) + RP = RP + (NREP - 1) * (LREP + 1) - 1 + AP = AP + LSET + SCORE = SCORE + NREP - 1 + END IF + ELSE IF(AMOS(AP:AP).EQ.'{') THEN + AP = AP + 1 + LSET = INDEX(AMOS(AP:),'}') - 1 + RP = RP + 1 + LSETS = LSET + CALL CACID(AMOS(AP:),CACIDS,LSET) + RS(RP:RP+LSET-1) = CACIDS(1:LSET) + LASTS = RP + RP = RP + LSET + AP = AP + LSETS + RS(RP:RP) = ',' + AP = AP + 1 + SCORE = SCORE + 1 + IF(AMOS(AP:AP).EQ.'(') THEN + AP = AP + 1 + LSET = INDEX(AMOS(AP:),')') - 1 + ICOMMA = INDEX(AMOS(AP:),',') + IF((ICOMMA.GT.0).AND.(ICOMMA.LT.LSET)) THEN + WRITE(*,*)'Variable gap in {}' + NVAR = NVAR + 1 + GO TO 5 + END IF + NREP = INTFC(AMOS(AP:),LSET) + LREP = RP - LASTS + RP = RP + 1 + CALL REPST(RS(LASTS:),RS(RP:),LREP,NREP) + RP = RP + (NREP - 1) * (LREP + 1) - 1 + AP = AP + LSET + SCORE = SCORE + NREP - 1 + END IF + ELSE IF(AMOS(AP:AP).EQ.'x') THEN + AP = AP + 1 + IF(AMOS(AP:AP).EQ.'(') THEN + AP = AP + 1 + LSET = INDEX(AMOS(AP:),')') - 1 + ICOMMA = INDEX(AMOS(AP:),',') + IF((ICOMMA.GT.0).AND.(ICOMMA.LT.LSET)) THEN + IF(RS(RP:RP).EQ.',') RP = RP - 1 + WTSNAM = WTSNAM(1:NAMLEN+IDIR-1)//'.WTS'//CSET(MOTIF:MOTIF) + WRITE(IDEV3,1002)WTSNAM(1:NAMLEN+IDIR+4) + CALL INTRP7(RS,RP,SCORE,WTSMAT,IDM,MAXLEN, + +CUTOFF,IOK) + IF(IOK.NE.0) WRITE(*,*)'SCREAM' + CALL OPENRS(IDEV4,WTSNAM(IDIR:),IOK,LRECL,1) + IF(IOK.NE.0) WRITE(*,*)'SCREAM, FILE OPENING' + TOP = SCORE + CALL WRTSCP(TITLE,SCORE,0,CUTOFF,TOP,IDM,WTSMAT,IDEV4) + NCOMMA = INTFC(AMOS(AP:),ICOMMA-1) + JCOMMA = INTFC(AMOS(AP+ICOMMA:),LSET-ICOMMA) + WRITE(IDEV3,1002)'A6 '// + +PATNAM(IDIR+2:IDIR+6)//' Class' + WRITE(IDEV3,1008)MOTIF +1008 FORMAT(I7,' Relative motif') + WRITE(IDEV3,1003)SCORE+NCOMMA+1 +1003 FORMAT(I7,' Relative start position') + WRITE(IDEV3,1004)JCOMMA-NCOMMA +1004 FORMAT(I7,' Number of extra positions') + AP = AP + LSET + RP = 0 + SCORE = 0 + MOTIF = MOTIF + 1 + ELSE + NCOMMA = INTFC(AMOS(AP:),LSET) + RP = RP + 1 + RS(RP:RP+NCOMMA-1) = COMMAS(1:NCOMMA) + RP = RP + NCOMMA - 1 + AP = AP + LSET + SCORE = SCORE + NCOMMA + END IF + ELSE + RP = RP + 1 + RS(RP:RP+1) = ',' + SCORE = SCORE + 1 + END IF + END IF + GO TO 10 + END IF + RP = RP - 1 + IF(RS(RP:RP).EQ.',') RP = RP - 1 + WTSNAM = WTSNAM(1:NAMLEN+IDIR-1)//'.WTS'//CSET(MOTIF:MOTIF) + WRITE(IDEV3,1002)WTSNAM(1:NAMLEN+IDIR+4) + CALL INTRP7(RS,RP,SCORE,WTSMAT,IDM,MAXLEN, + +CUTOFF,IOK) + IF(IOK.NE.0) WRITE(*,*)'SCREAM' + CALL OPENRS(IDEV4,WTSNAM(IDIR:),IOK,LRECL,1) + IF(IOK.NE.0) WRITE(*,*)'SCREAM, FILE OPENING' + TOP = SCORE + CALL WRTSCP(TITLE,SCORE,0,CUTOFF,TOP,IDM,WTSMAT,IDEV4) + WRITE(IDEV2,1002)PATNAM + GO TO 5 +100 CONTINUE + WRITE(*,*)'READ ERROR' + STOP +200 CONTINUE + WRITE(KBOUT,*)'Number of patterns',NMOTIF + WRITE(KBOUT,*)'Number of variable gaps',NVAR + END + SUBROUTINE REPST(S1,S2,L,N) + CHARACTER S1*(*),S2*(*) + J = 1 + DO 10 I = 1,N-1 + S2(J:J+L-1) = S1(1:L) + S2(J+L:J+L) = ',' + J = J + L + 1 +10 CONTINUE + END + INTEGER FUNCTION INTFC(STRING,LS) + CHARACTER TEMP*10,STRING*(*) + TEMP = STRING(1:LS) + CALL RJST(TEMP) + READ(TEMP,1000,ERR=10)INTFC +1000 FORMAT(I10) + RETURN +10 CONTINUE + INTFC = 0 + WRITE(*,*)'Error in encode' + END + SUBROUTINE CACID(ACID,REST,LACID) + CHARACTER ACID*(*),REST*20,ACIDS*20 + SAVE ACIDS + DATA ACIDS/'QWERTYIPASDFGHKLCVNM'/ + J = 0 + DO 10 I = 1,20 + DO 5 K = 1,LACID + IF(ACID(K:K).EQ.ACIDS(I:I)) GO TO 9 +5 CONTINUE + J = J + 1 + REST(J:J) = ACIDS(I:I) +9 CONTINUE +10 CONTINUE + LACID = J + END + LOGICAL FUNCTION ACID(CHAR) + LOGICAL ONEOF + CHARACTER ACIDS*(20),CHAR + SAVE ACIDS + EXTERNAL ONEOF + DATA ACIDS/'QWERTYIPASDFGHKLCVNM'/ + ACID = ONEOF(ACIDS,CHAR) + END + LOGICAL FUNCTION NUMBER(CHAR) + LOGICAL ONEOF + CHARACTER DIGITS*(10),CHAR + SAVE DIGITS + EXTERNAL ONEOF + DATA DIGITS/'1234567890'/ + NUMBER = ONEOF(DIGITS,CHAR) + END + LOGICAL FUNCTION ONEOF(CHARS,CHAR) + CHARACTER CHARS*(*),CHAR + ONEOF = .FALSE. + IF(INDEX(CHARS,CHAR).NE.0) ONEOF = .TRUE. + END + SUBROUTINE INTRP7(STRING,ISEND,LENGTH,WT,MAXCHR,MAXLEN, + +CUTOFF,IOK) + CHARACTER STRING*(*),TERM + INTEGER WT(MAXCHR,MAXLEN) + INTEGER CTONUM + EXTERNAL CTONUM + PARAMETER (TERM = ',') + IOK = 0 +C POINT TO STRING + IS = 1 + ICOL = 1 +C COUNT FILLED COLUMNS + CUTOFF = 0. +10 CONTINUE + CALL FILLI(WT(1,ICOL),MAXCHR,0) + CUTOFF = CUTOFF + 1. +20 CONTINUE + IF(IS.LE.ISEND)THEN + IF(STRING(IS:IS).NE.TERM)THEN + IROW = CTONUM(STRING(IS:IS)) + WT(IROW,ICOL) = 1 + IS = IS + 1 + GO TO 20 + END IF + NC = 1 +30 CONTINUE + IF(IS.LE.ISEND)THEN + IF(STRING(IS:IS).EQ.TERM)THEN + ICOL = ICOL + 1 + CALL FILLI(WT(1,ICOL),MAXCHR,0) + NC = NC + 1 + IS = IS + 1 + GO TO 30 + END IF +C +C END OF TERMINATORS +C +C IF(ICOL.GT.1) GO TO 10 +C ERROR TERMINATOR BEFORE ANY GOOD COLUMNS +C IOK = 1 +C RETURN + GO TO 10 + END IF +C STRING ENDED WITH TERMINATOR + LENGTH = ICOL - NC + 1 + RETURN + END IF +C +C END REACHED WITH NO TERMINATOR (NORMAL) + IF(ICOL.GT.0)THEN + LENGTH = ICOL + IOK = 0 + RETURN + END IF + IOK = 1 + END + SUBROUTINE WRTSCP(TITLE,LENGTH,MIDDLE,BOT,TOP,IDM, + +SUM,IDEV) + INTEGER TOT(120) + INTEGER SUM(IDM,LENGTH) + CHARACTER CHRSET*22,TITLE*(*) + SAVE CHRSET + DATA CHRSET/'CSTPAGNDEQBZHRKMILVFYW'/ +C PROTEIN MATRICES DONT WRITE ROWS FOR -X? AND SPACE SO SET DIMENSION +C TO IDM-4 + CALL FILLI(TOT,120,0) + WRITE(IDEV,1018)TITLE +1018 FORMAT(' ',A) +1019 FORMAT(' P',20I4) +1020 FORMAT(' N',20I4) +1021 FORMAT(' ',A,20I4) +1022 FORMAT(' ',2I6,2F10.3) + WRITE(IDEV,1022)LENGTH,MIDDLE,BOT,TOP + NLINES=1+(LENGTH-1)/20 + K1=1 + DO 400 J=1,NLINES + K2=MIN((K1+19),LENGTH) + WRITE(IDEV,1019)(K,K=K1-MIDDLE,K2-MIDDLE) + WRITE(IDEV,1020)(TOT(K),K=K1,K2) + DO 390 I=1,IDM-4 + WRITE(IDEV,1021)CHRSET(I:I),(SUM(I,K),K=K1,K2) +390 CONTINUE + K1=K1+20 + IF(K1.GT.LENGTH)K1=LENGTH +400 CONTINUE + CLOSE(UNIT=IDEV) + END diff --git a/src/staden/subs89.f b/src/staden/subs89.f new file mode 100644 index 0000000..393f055 --- /dev/null +++ b/src/staden/subs89.f @@ -0,0 +1,1659 @@ +C general subroutines +C author rodger staden +C 14-2-90 changed titout to use gtstr (was using getstr) +C and returned inflag to wrtact +C 16-3-90 replaced read in bpause by call to sin +C 18-4-90 added nmmtch for comparing strings (used by pir reading +C routines to compare entry names) +C 4-7-90 added routines shofu, showfi,errom,busy +C 4-7-90 removed all routines dealing with sequence libraries +C and put them into seqlibsubs.for +C 4-7-90 added radion, checkb +C 6-7-90 removed all routines related to userinterface +C 23-7-90 moved sethlp, help, help2 to userface +C put hqn back for sap +C 10-12-90 Modified 2 lines in nmmtch to check for empty strings +C 24-4-91 Added new routines +C 18-11-91 moved openf1 from userface. deleted in userface and dialogues +C also changed it to allow file deletion using delf +C 2-3-92 Altered ttext +C 10-4-92 made enco more efficient + INTEGER FUNCTION HQN(STRING) + CHARACTER STRING*(*),EM,QM + PARAMETER (EM='!',QM='?') + EXTERNAL NOTLR +C AUTHOR: RODGER STADEN +C RETURNS: +C 1 FOR HELP +C 2 FOR QUIT +C 3 FOR ALL BLANKS +C 0 OTHERWISE + IF(STRING(1:1).EQ.QM) THEN + HQN = 1 + RETURN + END IF + IF(STRING(1:1).EQ.EM) THEN + HQN = 2 + RETURN + END IF + I = LEN(STRING) + IN = NOTLR(STRING,I,' ') + IF(IN.EQ.0) THEN + HQN = 3 + RETURN + END IF + HQN = 0 + END + SUBROUTINE RJST(STRING) + CHARACTER STRING*(*) + EXTERNAL NOTIRL + L = LEN(STRING) + I = NOTIRL(STRING,L,' ') + IF(I.NE.L) THEN + J = L + DO 10 K = I,1,-1 + STRING(J:J) = STRING(K:K) + J = J - 1 +10 CONTINUE +C MOVED DISTANCE L - I CHARS, SO FILL WITH SPACES + I = L - I + STRING(1:I) = ' ' + END IF + END + SUBROUTINE MARGC1(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT, + +HELPS,HELPE,HELPF,IDEVH,KBIN,KBOUT) +C AUTHOR RODGER STADEN + INTEGER HELPS,HELPE + CHARACTER HELPF*(*) + WRITE(KBOUT,8006)ISYMAX,MARGB,MARGT +8006 FORMAT(' y coordinates first:',/, + +' screen height=',I6,' start position=',I5, + + ' height=',I5) + IBOT = MARGB + MNM = 1 + MXM = ISYMAX + CALL GETINT(MNM,MXM,IBOT,'start height', + +IVAL,KBIN,KBOUT,HELPS,HELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MARGB = IVAL + ITOP = MIN(ISYMAX-MARGB,MARGT) + MNM = MIN(ISYMAX/100,ITOP) + MXM = ISYMAX-MARGB + CALL GETINT(MNM,MXM,ITOP,'height', + +IVAL,KBIN,KBOUT,HELPS,HELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MARGT = IVAL + WRITE(KBOUT,8009)ISXMAX,MARGL,MARGR +8009 FORMAT(' x coordinates:',/, + +' screen width=',I6,' start position=',I5, + + ' width=',I5) + IBOT = MARGL + MNM = 1 + MXM = ISXMAX + CALL GETINT(MNM,MXM,IBOT,'start position', + +IVAL,KBIN,KBOUT,HELPS,HELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MARGL = IVAL + ITOP = MIN(ISXMAX-MARGL,MARGR) + MNM = MIN(ISXMAX/100,ITOP) + MXM = ISXMAX-MARGL + CALL GETINT(MNM,MXM,ITOP,'width', + +IVAL,KBIN,KBOUT,HELPS,HELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + MARGR = IVAL + END + INTEGER FUNCTION MC(SEQ1,SEQ2,LENGTH) + CHARACTER SEQ1(LENGTH),SEQ2(LENGTH) + EXTERNAL IUBM1 + MC = 1 + DO 10 I = 1,LENGTH + IF(IUBM1(SEQ2(I),SEQ1(I)).EQ.0) RETURN +10 CONTINUE + MC = 0 + END + INTEGER FUNCTION GENRCI(STRING,LENS,LENI,C,LC,NENTRY) + PARAMETER (LENM = 6) + CHARACTER STRING(LENS) + INTEGER REDS(17),REDE(17),RED(37),KS(LENM) + INTEGER LS(LENM),II(LENM),C(0:LC) + EXTERNAL NCODEA + SAVE +C T,C,A,G,- R Y W S M K H B V D +C +C 1,2,3,4,5,6,7,8,9,0,1,2,3,4,5,6,7,8,9,0,1,2,3,4, + DATA RED/1,2,3,4,1,2,3,4,3,4,1,2,1,3,2,4,2,3,1,4,1,2,3,1, +C 5,6,7,8,9,0,1,2,3,4,5,6,7 + + 2,4,2,3,4,1,3,4,1,2,3,4,5/ +C - R Y W S M K H B V D N ? + DATA REDS/1,2,3,4,5, 9,11,13,15,17,19,21,24,27,30,33,37/ + DATA REDE/1,2,3,4,8,10,12,14,16,18,20,23,26,29,32,36,37/ + IENTRY = NENTRY + NENTRY = 1 + IF(IENTRY.NE.0) GO TO 2 + CALL FILLI(KS,LENI,16) + CALL CON17(STRING,KS,MIN(LENS,LENI)) + NEXT = 1 + II(1) = REDS(KS(1)) - 1 +1 CONTINUE + II(NEXT) = II(NEXT) + 1 + LS(NEXT) = RED(II(NEXT)) + IF(NEXT.LT.LENI) THEN + NEXT = NEXT + 1 + II(NEXT) = REDS(KS(NEXT)) - 1 + GO TO 1 + END IF +C WRITE(*,*)LS + GENRCI = NCODEA(LS,LENI,C,5,LC) + RETURN +2 CONTINUE + DO 3 I = LENI,1,-1 + IF(II(I).LT.REDE(KS(I))) THEN + NEXT = I + GO TO 1 + END IF +3 CONTINUE + GENRCI = 0 + END + SUBROUTINE SETCN(C,LENGTH,IDM,LC) + INTEGER C(0:LC) + C(0) = 0 + K = IDM - 1 + N = 0 + DO 10 I = 1,LENGTH + M = K**(I-1) + C(0) = C(0) - C(N) + DO 5 J = 1,K + N = N + 1 + C(N) = J*M +5 CONTINUE +10 CONTINUE + END + INTEGER FUNCTION NCODEA(SEQ,LENGTH,C,IDM,LC) + INTEGER SEQ(LENGTH),C(0:LC) + NCODEA = 0 + N = C(0) + J = 0 +C NEXT LINE NEW 4-7-89 + K = IDM - 1 + DO 10 I = 1,LENGTH + L = SEQ(I) + IF(L.EQ.IDM) RETURN + N = N + C(J+L) +C NEXT LINE NEW 4-7-89 (WAS J = J + 4) + J = J + K +10 CONTINUE + NCODEA = N + END + SUBROUTINE ENCOND(SEQ,IDIM,WORDP,IDE,IDCHAR,CONSTS,LENGTH, + +LCONST) +C AUTHOR RODGER STADEN + INTEGER SEQ(IDIM),WORDP(IDE),CONSTS(0:LCONST) + INTEGER NCODEA + EXTERNAL NCODEA +C ENCODES A SEQUENCE OF LENGTH IDIM AND CHARACTERSET SIZE IDCHAR +C INTO TWO ARRAYS: WORDP(I) CONTAINS THE POSITION OF THE FIRST OCCURRENCE +C OF WORD(I), SEQ(I) CONTAINS A LINKED LIST OF SECOND, THIRD,... OCCURENCES +C OF WORD + CALL FILLI(WORDP,IDE,0) + DO 20 I = 1, IDIM-LENGTH+1 + J = NCODEA(SEQ(I),LENGTH,CONSTS,IDCHAR,LCONST) + SEQ(I) = 0 + IF(J.NE.0) THEN + J1 = WORDP(J) + IF(J1.EQ.0) THEN + WORDP(J) = I + ELSE +10 CONTINUE + J2 = J1 + J1 = SEQ(J2) + IF(J1.NE.0) GO TO 10 + SEQ(J2) = I + END IF + END IF +20 CONTINUE + END + SUBROUTINE CON17(S,N,L) + CHARACTER S(L) + INTEGER N(L),DTONUM + EXTERNAL DTONUM + DO 1 I = 1,L + N(I) = DTONUM(S(I)) +1 CONTINUE + END + SUBROUTINE SEQEDT(SEQ,MAXSEQ,ISEQ,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IDEV,FILNAM,FILEIN,IDM,IOK) + CHARACTER LINE*133,FILNAM*(*),FILEIN*(*),SEQ(MAXSEQ),HELPF*(*) + CHARACTER CHARG1*14,CHARG2*48 + PARAMETER (CHARG1='TUCAG N-tucagn') + PARAMETER + +(CHARG2='ABCDEFGHIKLMNPQRSTVWXYZabcdefghiklmnpqrstvwxyz- ') + PARAMETER (NGOOD1 = 14, NGOOD2 = 48) + EXTERNAL NOTRL + IOK = 1 +10 CONTINUE + WRITE(KBOUT,1000) +1000 FORMAT(' Name of file to edit.') + LIN = LEN(FILNAM) + LIN = NOTRL(FILNAM,LIN,' ') + CALL GTSTR('File name',FILNAM,FILEIN,LIN,KBOUT,KBIN,INFLAG) + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + IF(LIN.EQ.0) FILEIN = FILNAM + CALL CEDIT(FILEIN) + CALL YESNO(IWANT,'Make edited sequence active', + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IOK = 1 + IF(IWANT.NE.0) RETURN + CALL OPENRS(IDEV,FILEIN,IOK,LRECL,2) + IF(IOK.NE.0) RETURN + IF(IDM.EQ.5) THEN + CALL FILTF(SEQ,MAXSEQ,ISEQ,LINE,IDEV,KBOUT,CHARG1,NGOOD1,IOK) + ELSE IF(IDM.EQ.26) THEN + CALL FILTF(SEQ,MAXSEQ,ISEQ,LINE,IDEV,KBOUT,CHARG2,NGOOD2,IOK) + END IF + CLOSE(UNIT=IDEV) + END + SUBROUTINE FILTF(SEQ,MAXSEQ,ISEQ,LINE,IDEV,KBOUT,CHARG,NGOOD,IOK) + CHARACTER SEQ(MAXSEQ) + CHARACTER LINE*(*),CHARG*(*) + EXTERNAL LOK + ISEQ = 0 + LNO = 0 + IOK = 1 +10 CONTINUE + LNO = LNO + 1 + READ(IDEV,1000,ERR=100,END=200)LINE +1000 FORMAT(A) + IOK = LOK(LINE,CHARG,NGOOD) + IF(IOK.EQ.0) THEN + DO 5 I = 1,LEN(LINE) + IF(LINE(I:I).NE.' ') THEN + ISEQ = ISEQ + 1 + IF(ISEQ.GT.MAXSEQ) THEN + WRITE(KBOUT,1001)MAXSEQ +1001 FORMAT(' Maximum sequence length',I6,' exceeded') + RETURN + END IF + SEQ(ISEQ) = LINE(I:I) + END IF +5 CONTINUE + END IF + GO TO 10 +100 CONTINUE + WRITE(KBOUT,1002)LNO +1002 FORMAT(' Error reading line',I6,' of file') + RETURN +200 CONTINUE + WRITE(KBOUT,1003)ISEQ +1003 FORMAT(' Sequence length is now',I6) + IOK = 0 + END + INTEGER FUNCTION LOK(LINE,OKC,NOKC) + CHARACTER LINE*(*),OKC*(*) + INTEGER COK + EXTERNAL COK + LOK = 1 + DO 10 I = 1,LEN(LINE) + IF(COK(LINE(I:I),OKC,NOKC).NE.0) RETURN +10 CONTINUE + LOK = 0 + END + INTEGER FUNCTION COK(CHAR,OKC,NOKC) + CHARACTER CHAR,OKC*(*) + COK = 0 + DO 10 I = 1,NOKC + IF(CHAR.EQ.OKC(I:I)) RETURN +10 CONTINUE + COK = 1 + END + INTEGER FUNCTION NOTILR(TEXT,ITEXT,WORD) +C AUTHOR: RODGER STADEN +C LOOKS LEFT TO RIGHT THRU TEXT FOR FIRST ELEMENT THAT IS NOT WORD +C RETURNS ELEMENT NUMBER OR ZERO IF ALL ELEMENTS ARE WORD + CHARACTER TEXT*(*),WORD + NOTILR = 1 + DO 1 I=1,ITEXT + NOTILR = I + IF(TEXT(I:I).NE.WORD)RETURN +1 CONTINUE + END + INTEGER FUNCTION NOTIRL(TEXT,ITEXT,WORD) +C AUTHOR: RODGER STADEN +C LOOKS RIGHT TO LEFT THRU TEXT FOR FIRST ELEMENT THAT IS NOT WORD +C RETURNS ELEMENT NUMBER OR ZERO IF ALL ELEMENTS ARE WORD + CHARACTER TEXT*(*),WORD + NOTIRL = ITEXT + DO 1 I=ITEXT,1,-1 + NOTIRL = I + IF(TEXT(I:I).NE.WORD)RETURN +1 CONTINUE + END + INTEGER FUNCTION NOTRLA(TEXT,ITEXT,WORD) +C AUTHOR: RODGER STADEN +C LOOKS RIGHT TO LEFT THRU TEXT FOR FIRST ELEMENT THAT IS NOT WORD +C RETURNS ELEMENT NUMBER OR ZERO IF ALL ELEMENTS ARE WORD + CHARACTER TEXT(ITEXT),WORD + DO 1 I=ITEXT,1,-1 + NOTRLA = I + IF(TEXT(I).NE.WORD)RETURN +1 CONTINUE + NOTRLA = 0 + END + INTEGER FUNCTION NOTLRA(TEXT,ITEXT,WORD) +C AUTHOR: RODGER STADEN +C LOOKS LEFT TO RIGHT THRU TEXT FOR FIRST ELEMENT THAT IS NOT WORD +C RETURNS ELEMENT NUMBER OR ITEXT + 1 IF ALL ELEMENTS ARE WORD + CHARACTER TEXT(ITEXT),WORD + DO 1 I=1,ITEXT + NOTLRA = I + IF(TEXT(I).NE.WORD)RETURN +1 CONTINUE + NOTLRA = ITEXT + 1 + END + SUBROUTINE WRTACT(IDEV,FILNAM,KBIN,KBOUT, + +SEQ,IDIM1,IHELPS,IHELPE,HELPF,IDEVH) + CHARACTER SEQ(IDIM1),FILNAM*(*),HELPF*(*) + CALL OPENF1(IDEV,FILNAM,1,IOK,KBIN,KBOUT, + +'File name for active sequence', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.EQ.0)THEN + CALL TITOUT(IDEV,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.2)CALL FMTDK(IDEV,SEQ,IDIM1) + CLOSE(UNIT=IDEV) + END IF + END + SUBROUTINE GSPIN(MINSP,MAXSP,SPAN,MINIW,MAXIW,IWRIT,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) +C AUTHOR: RODGER STADEN + CHARACTER HELPF*(*) + INTEGER SPAN,VALUE + IOK = 1 +20 CONTINUE + CALL GETINT(MINSP,MAXSP,SPAN,'odd span length', + +VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0)RETURN + IF(MOD(VALUE,2).EQ.0)GO TO 20 + SPAN = VALUE + CALL GETINT(MINIW,MAXIW,IWRIT,'plot interval', + +VALUE,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0)RETURN + IWRIT = VALUE + END + SUBROUTINE GTSCR(STRING,IDIM,MATRIX,IDM,SMIN,SMAX) +C AUTHOR RODGER STADEN + INTEGER MATRIX(IDM,IDM),SMIN,SMAX + CHARACTER STRING(IDIM) + INTEGER DTONUM + EXTERNAL DTONUM + SMIN = 0 + SMAX = 0 + DO 10 I = 1,IDIM + K1 = 99999 + K2 = -99999 + L = DTONUM(STRING(I)) + DO 5 J = 1,IDM + M = MATRIX(L,J) + K1 = MIN(K1,M) + K2 = MAX(K2,M) +5 CONTINUE + SMIN = SMIN + K1 + SMAX = SMAX + K2 +10 CONTINUE + END + SUBROUTINE ADDR(FROM,TO,ID) +C AUTHOR: RODGER STADEN + REAL FROM(ID),TO(ID) + DO 10 I = 1,ID + TO(I) = TO(I) + FROM(I) +10 CONTINUE + END + SUBROUTINE ADDI(FROM,TO,ID) +C AUTHOR: RODGER STADEN + INTEGER FROM(ID),TO(ID) + DO 10 I = 1,ID + TO(I) = TO(I) + FROM(I) +10 CONTINUE + END + SUBROUTINE COPYR(FROM,TO,ID) +C AUTHOR: RODGER STADEN + REAL FROM(ID),TO(ID) + DO 10 I = 1,ID + TO(I) = FROM(I) +10 CONTINUE + END + SUBROUTINE GTREG(KBIN,KBOUT,J1,J2,I1,I2,P, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + CHARACTER HELPF*(*),P*(*) +C AUTHOR: RODGER STADEN + IOK = 1 + MININ = J1 + MAXIN = J2 + WRITE(KBOUT,1000)P +1000 FORMAT(' ',A) + CALL GETINT(MININ,MAXIN,I1,'start',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + IF(IVAL.NE.I1) THEN + I2 = J2 + END IF + I1 = IVAL + IF(I1.EQ.0) RETURN + MININ = I1 + 1 + MAXIN = J2 + CALL GETINT(MININ,MAXIN,I2,'end',IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) RETURN + I2 = IVAL + IOK = 0 + END + SUBROUTINE CCASEA(STRING,L,IFLAG) + CHARACTER STRING(L),TUPPER,TLOWER + EXTERNAL TUPPER,TLOWER +C AUTHOR RODGER STADEN + IF(IFLAG.EQ.1)THEN + DO 10 I = 1,L + STRING(I) = TUPPER(STRING(I)) +10 CONTINUE + ELSE IF(IFLAG.EQ.2)THEN + DO 20 I = 1,L + STRING(I) = TLOWER(STRING(I)) +20 CONTINUE + END IF + END + INTEGER FUNCTION INDEXA(STRING,ID,CHAR) + CHARACTER STRING(ID),CHAR +C FUNCTION TO FIND FIRST OCCURRENCE OF CHAR IN STRING + DO 10 I = 1,ID + IF(STRING(I).EQ.CHAR)THEN + INDEXA = I + RETURN + END IF +10 CONTINUE + INDEXA = 0 + END + SUBROUTINE TITOUT(IDEV,KBIN,KBOUT,IHELPS,IHELPE,HELPF, + +IDEVH,INFLAG) +C AUTHOR: RODGER STADEN + CHARACTER LINE*60,HELPF*(*) +10 CONTINUE + WRITE(KBOUT,1000) +1000 FORMAT(' You may give your sequence a one line title.') + LIN = 0 + CALL GTSTR('Title',' ',LINE,LIN,KBOUT,KBIN,INFLAG) + IF(LIN.LT.1) RETURN + IF(INFLAG.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + GO TO 10 + END IF + IF(INFLAG.EQ.2) RETURN + WRITE(IDEV,1004,ERR=20)LINE(1:LIN) +1004 FORMAT(';',A) +20 CONTINUE + END + SUBROUTINE TTEXT(IDEV,FILNAM,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),HELPF*(*) + CALL OPENF1(IDEV,FILNAM,0,IOK,KBIN,KBOUT, + +'Name of file to read', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL TTEXT1(IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IQUIT) + END + SUBROUTINE TTEXT1(IDEV,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IQUIT) +C AUTHOR: RODGER STADEN + PARAMETER (IPAGE=22) + CHARACTER LINE*80,HELPF*(*),SPACE + INTEGER NOTIRL + EXTERNAL NOTIRL + SAVE SPACE + DATA SPACE/' '/ +1002 FORMAT(' ',A) +C COUNT LINES OUTPUT + LINDON=0 + IDONE=0 +10 CONTINUE + READ(IDEV,1003,ERR=110,END=30)LINE +1003 FORMAT(A) + WRITE(KBOUT,1002)LINE(1:MAX(1,NOTIRL(LINE,79,SPACE))) + IDONE=IDONE+1 + LINDON=LINDON+1 + IF(IDONE.GE.IPAGE)THEN + CALL BPAUSE(KBIN,KBOUT,IQUIT) + IF(IQUIT.NE.0) GO TO 50 + IDONE=0 + END IF + GO TO 10 +30 CONTINUE + WRITE(KBOUT,1005) +1005 FORMAT(' End of file') + CALL BPAUSE(KBIN,KBOUT,IQUIT) +50 CONTINUE + CLOSE(UNIT=IDEV) + RETURN +110 CONTINUE + WRITE(KBOUT,1010) +1010 FORMAT(' Error reading file') + CLOSE(UNIT=IDEV) + END + SUBROUTINE CCASE(STRING,IFLAG) + CHARACTER STRING*(*),TUPPER,TLOWER + EXTERNAL TUPPER,TLOWER +C AUTHOR RODGER STADEN + L = LEN(STRING) + IF(IFLAG.EQ.1)THEN + DO 10 I = 1,L + STRING(I:I) = TUPPER(STRING(I:I)) +10 CONTINUE + ELSE IF(IFLAG.EQ.2)THEN + DO 20 I = 1,L + STRING(I:I) = TLOWER(STRING(I:I)) +20 CONTINUE + END IF + END + CHARACTER*1 FUNCTION TUPPER(CHAR1) +C AUTHOR RODGER STADEN + CHARACTER UP*26,LOW*26,CHAR1 + SAVE LOW,UP + DATA UP/ 'ABCDEFGHIJKLMNOPQRSTUVWXYZ' / + DATA LOW/ 'abcdefghijklmnopqrstuvwxyz' / + I = INDEX(LOW,CHAR1) + TUPPER = CHAR1 + IF (I.NE.0) TUPPER = UP(I:I) + END + CHARACTER*1 FUNCTION TLOWER(CHAR1) +C AUTHOR RODGER STADEN + CHARACTER UP*26,LOW*26,CHAR1 + SAVE LOW,UP + DATA UP/ 'ABCDEFGHIJKLMNOPQRSTUVWXYZ' / + DATA LOW/ 'abcdefghijklmnopqrstuvwxyz' / + I = INDEX(UP,CHAR1) + TLOWER = CHAR1 + IF (I.NE.0) TLOWER = LOW(I:I) + END + INTEGER FUNCTION NOTLR(TEXT,ITEXT,WORD) +C AUTHOR: RODGER STADEN +C LOOKS LEFT TO RIGHT THRU TEXT FOR FIRST ELEMENT THAT IS NOT WORD +C RETURNS ELEMENT NUMBER OR ZERO IF ALL ELEMENTS ARE WORD + CHARACTER TEXT*(*),WORD + DO 1 I=1,ITEXT + IF(TEXT(I:I).NE.WORD)THEN + NOTLR = I + RETURN + END IF +1 CONTINUE + NOTLR = 0 + END + INTEGER FUNCTION NOTRL(TEXT,ITEXT,WORD) +C AUTHOR: RODGER STADEN +C LOOKS RIGHT TO LEFT THRU TEXT FOR FIRST ELEMENT THAT IS NOT WORD +C RETURNS ELEMENT NUMBER OR ZERO IF ALL ELEMENTS ARE WORD + CHARACTER TEXT*(*),WORD + DO 1 I=ITEXT,1,-1 + IF(TEXT(I:I).NE.WORD)THEN + NOTRL=I + RETURN + END IF +1 CONTINUE + NOTRL = 0 + END + CHARACTER*3 FUNCTION TRANF3(CODON,PAA,CODE) +C AUTHOR RODGER STADEN + CHARACTER CODON(3),PAA(5,5,5),THREEL*3,TRANF,THREE*3 + INTEGER CODE + EXTERNAL THREEL,TRANF + THREE(1:3)=' ' + IF(CODE.EQ.3)THEN + THREE(1:3)=THREEL(TRANF(CODON,PAA)) + ELSE + THREE(2:2)=TRANF(CODON,PAA) + END IF + TRANF3=THREE + RETURN + END +C TRANB3 + CHARACTER*3 FUNCTION TRANB3(CODON,PAA,CODE) +C AUTHOR RODGER STADEN + CHARACTER CODON(3),PAA(5,5,5),THREEL*3,TRANB,THREE*3 + INTEGER CODE + EXTERNAL THREEL,TRANB + THREE=' ' + IF(CODE.EQ.3)THEN + THREE(1:3)=THREEL(TRANB(CODON,PAA)) + ELSE + THREE(2:2)=TRANB(CODON,PAA) + END IF + TRANB3=THREE(1:3) + RETURN + END +C TRANF + CHARACTER*1 FUNCTION TRANF(CODON,PAA) +C AUTHOR: RODGER STADEN + CHARACTER CODON(3),PAA(5,5,5) + INTEGER IC(3),CTONUM + EXTERNAL CTONUM +C + DO 10 I=1,3 + IC(I)=CTONUM(CODON(I)) +10 CONTINUE + TRANF=PAA(IC(3),IC(2),IC(1)) + RETURN + END +C TRANB + CHARACTER*1 FUNCTION TRANB(CODON,PAA) +C AUTHOR: RODGER STADEN + CHARACTER*1 CODON(3),PAA(5,5,5) + INTEGER IC(3),CTONUM,ICOMP + EXTERNAL CTONUM,ICOMP +C + DO 10 I=1,3 + IC(I)=CTONUM(CODON(I)) +10 CONTINUE + TRANB=PAA(ICOMP(IC(1)),ICOMP(IC(2)),ICOMP(IC(3))) + RETURN + END +C THREEL + CHARACTER*3 FUNCTION THREEL(ONEL) + CHARACTER ONEL,ALLONE*21 + CHARACTER*3 THREES(21) + SAVE ALLONE,THREES + DATA ALLONE/'ACDEFGHIKLMNPQRSTVWY*'/ + DATA THREES/ + +'Ala','Cys','Asp','Glu','Phe','Gly','His','Ile','Lys', + +'Leu','Met','Asn','Pro','Gln','Arg','Ser','Thr','Val', + +'Trp','Tyr','***'/ + THREEL=' ' + DO 10 I=1,21 + IF(ONEL.EQ.ALLONE(I:I))THEN + THREEL=THREES(I)(1:3) + RETURN + END IF +10 CONTINUE + RETURN + END + SUBROUTINE MWCALC(SEQ,IDIM,J1,J2,KBOUT,IDEV) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),CHRSET(26) + REAL AAWTS(26),AWTSUM(26),ASUM(26) + INTEGER CTONUM + EXTERNAL CTONUM + SAVE CHRSET,AAWTS + DATA CHRSET/ + +'C','S','T','P','A','G','N', + +'D','E','Q','B','Z','H','R', + +'K','M','I','L','V','F','Y', + +'W','-','X','?',' '/ +C VALUES CHANGED 26-2-91 + DATA AAWTS/103.1388,87.0782,101.1051,97.1167,71.0788,57.0519, + + 114.1038, + + 115.0886,129.1155,128.1307,0.0,0.0,137.1411,156.1875, + + 128.1714,131.1926,113.1594,113.1594,99.1326,147.1766, + + 163.1760, + + 186.2132,0.0,0.0,0.0,0.0/ +C DATA AAWTS/103.15,87.09,101.12,97.13,71.09,57.07,114.12, +C + 115.10,129.13,128.15,0.0,0.0,137.16,156.21, +C + 128.19,131.22,113.18,113.18,99.15,147.19,163.19, +C + 186.22,0.0,0.0,0.0,0.0/ + WRITE(KBOUT,*)' Sequence composition' + REALN = J2 - J1 + 1 + REALN = 100.0/REALN + SUMWT=0. +C SUMPOL=0. + DO 20 I=1,26 + ASUM(I)=0. + AWTSUM(I)=0. +20 CONTINUE + DO 30 I=J1,J2 + IACID=CTONUM(SEQ(I)) + ASUM(IACID)=ASUM(IACID)+1. + AWTSUM(IACID)=AWTSUM(IACID)+AAWTS(IACID) +C AWTSUM(IACID)=AWTSUM(IACID)+AAWTS(IACID)-0.015 +C SUMPOL=SUMPOL+POLAR(IACID) +C SUMWT=SUMWT+AAWTS(IACID)-0.015 + SUMWT=SUMWT+AAWTS(IACID) +30 CONTINUE +C ADD ON 1 WATER MOLECULE + SUMWT=SUMWT+18.0152 + WRITE(IDEV,1000)(CHRSET(K),K=1,13) + WRITE(IDEV,1001)(ASUM(K),K=1,13) + WRITE(IDEV,1004)(REALN*ASUM(K),K=1,13) + WRITE(IDEV,1002)(AWTSUM(K),K=1,13) + WRITE(IDEV,1000)(CHRSET(K),K=14,26) + WRITE(IDEV,1001)(ASUM(K),K=14,26) + WRITE(IDEV,1004)(REALN*ASUM(K),K=14,26) + WRITE(IDEV,1002)(AWTSUM(K),K=14,26) +1000 FORMAT(/' A',13(3X,1A,2X)) +1001 FORMAT(' N',13(1X,F4.0,1X)) +1002 FORMAT(' W',13F6.0) +1004 FORMAT(' %',13F6.1) + WRITE(IDEV,1003)SUMWT +1003 FORMAT(' Total molecular weight=',F12.3) + RETURN + END +C + SUBROUTINE BCOMP(SEQ,IDIM,J1,J2,KSTART,IDEV) +C AUTHOR RODGER STADEN + CHARACTER SEQ(IDIM) + REAL TOT(5) + INTEGER CTONUM + EXTERNAL CTONUM + WRITE(IDEV,*)' Sequence composition' + DO 10 I=1,5 + TOT(I)=0.0 +10 CONTINUE + DO 20 I=J1-KSTART+1,J2-KSTART+1 + J=CTONUM(SEQ(I)) + TOT(J)=TOT(J)+1. +20 CONTINUE + WRITE(IDEV,1000) +1000 FORMAT(' ',10X,'T',10X,'C',10X,'A',10X,'G',10X,'-') + WRITE(IDEV,1001)TOT +1001 FORMAT(' ',1X,5(F10.0,1X)) + T=100.0/(J2-J1+1) + WRITE(IDEV,1002)(TOT(K)*T,K=1,5) +1002 FORMAT(' ',2X,5(F10.1,'%')) + RETURN + END +C SUBROUTINE TO READ CHARACTER DATA FROM IDEV, REMOVE SPACES, FILL +C ARRAY AND RETURN NUMBER OF ELEMENTS USED. ANY LINES STARTING WITH +C A ; ARE TREATED AS COMMENTS AND WRITTEN TO DEVICE KBOUT + SUBROUTINE ARRFIL(IDEV,SEQNCE,J,KBOUT) +C 14-8-91 Added err= option to read, and set length to 0 if error found +C AUTHOR: RODGER STADEN + CHARACTER TEMP(80),SEQNCE(J) + CHARACTER SPACE,ENDCHR,TITCHR + SAVE ENDCHR,SPACE,TITCHR + DATA ENDCHR/'@'/ + DATA SPACE/' '/ + DATA TITCHR/';'/ + IDMX=J + J=0 +1 CONTINUE + READ(IDEV,1001,END=30,ERR=40)TEMP +1001 FORMAT(80A1) + IF(TEMP(1).EQ.TITCHR)THEN + WRITE(KBOUT,1003)(TEMP(K),K=2,80) +1003 FORMAT(' ',79A1) + GO TO 1 + END IF +10 CONTINUE + DO 20 I=1,80 + IF(TEMP(I).NE.SPACE)THEN + IF(TEMP(I).EQ.ENDCHR)RETURN + IF(J.EQ.IDMX)THEN + WRITE(KBOUT,1002)IDMX +1002 FORMAT( + + ' Too much data. Maximum possible', + + ' =',I6,', input stopped there') + RETURN + END IF + J=J+1 + SEQNCE(J)=TEMP(I) + END IF +20 CONTINUE + GO TO 1 +30 CONTINUE + RETURN + 40 CONTINUE + CALL ERROM(KBOUT,'Error reading file') + J = 0 + END +C BUB2AS +C SUBROUTINE TO SORT INTEGER ARRAY (LIST) INTO ASCENDING ORDER + SUBROUTINE BUB2AS(LIST,LIST2,IDIM) +C AUTHOR: RODGER STADEN + INTEGER LIST(IDIM),LIST2(IDIM) + I=0 + J=0 +10 CONTINUE +C SET I=J IF WE HAVE JUST CORRECTLY POSITIONED AN ELEMENT + IF(J.GT.I)I=J + I=I+1 + IF(I.EQ.IDIM)RETURN +20 CONTINUE + IF(LIST(I).LE.LIST(I+1))GO TO 10 +C FIRST MOVE THIS ELEMENT? IF SO SET POINTER TO ITS INITIAL POSITION + IF(J.LT.I)J=I + ITEMP=LIST(I) + LIST(I)=LIST(I+1) + LIST(I+1)=ITEMP + ITEMP=LIST2(I) + LIST2(I)=LIST2(I+1) + LIST2(I+1)=ITEMP +C DECREMENT BACK THRU LIST WITH THIS ELEMENT + IF(I.GT.1)I=I-1 + GO TO 20 + END + SUBROUTINE BUB3AS(LIST,LIST2,LIST3,IDIM) +C AUTHOR: RODGER STADEN + INTEGER LIST(IDIM),LIST2(IDIM),LIST3(IDIM) + I=0 + J=0 +10 CONTINUE +C SET I=J IF WE HAVE JUST CORRECTLY POSITIONED AN ELEMENT + IF(J.GT.I)I=J + I=I+1 + IF(I.EQ.IDIM)RETURN +20 CONTINUE + IF(LIST(I).LE.LIST(I+1))GO TO 10 +C FIRST MOVE THIS ELEMENT? IF SO SET POINTER TO ITS INITIAL POSITION + IF(J.LT.I)J=I + ITEMP=LIST(I) + LIST(I)=LIST(I+1) + LIST(I+1)=ITEMP + ITEMP=LIST2(I) + LIST2(I)=LIST2(I+1) + LIST2(I+1)=ITEMP + ITEMP=LIST3(I) + LIST3(I)=LIST3(I+1) + LIST3(I+1)=ITEMP +C DECREMENT BACK THRU LIST WITH THIS ELEMENT + IF(I.GT.1)I=I-1 + GO TO 20 + END +C CHARCT +C SUBROUTINE TO COUNT NUMBRE OF CHARS BETWSEN ENCLOSING /S + SUBROUTINE CHARCT(CHARS,IDIM,PCHAR,NCHAR) +C AUTHOR: RODGER STADEN + CHARACTER CHARS(IDIM),SLASH + INTEGER PCHAR + SAVE SLASH + DATA SLASH/'/'/ + NCHAR=-1 +C +10 CONTINUE + NCHAR=NCHAR+1 + I=NCHAR+PCHAR + IF(I.EQ.IDIM)RETURN + IF(CHARS(I).EQ.SLASH)RETURN + GO TO 10 + END +C encona +C routine to store positions of words in posns and first occurences +C in wordp and number of occurences in wordn +C each number is a value representing one of the le4 possible +C words of length length made up of 4 characters +C words in posns are numbers from 1 to 4**length + SUBROUTINE ENCONA(POSNS,IDIM,WORDP,WORDN,LE4,LENGTH) +C AUTHOR: RODGER STADEN + INTEGER WORDP(LE4),POSNS(IDIM) + INTEGER WORDN(LE4) +C number of words of length length + IDIM1 = IDIM - (LENGTH-1) + DO 10 I=1,LE4 + WORDN(I) = 0 +10 CONTINUE +C loop for each word + DO 100 I=1,IDIM1 + N = POSNS(I) + IF(N.EQ.0)GO TO 100 +C is their already an entry for this word? + IF(WORDN(N).NE.0)GO TO 60 +C first entry, put in wordp + WORDP(N)=I + GO TO 80 +60 CONTINUE +C need to chain along posn until find correct posn for i +C first posn is in wordp(n) + IP=WORDP(N) + DO 70 J=2,WORDN(N) +70 IP=POSNS(IP) +C so put this i at posns(ip) + POSNS(IP)=I +80 CONTINUE +C now increment number of occurences of n + WORDN(N)=WORDN(N)+1 +100 CONTINUE + RETURN + END +C routine to turn a sequence of 1,2,3,4,0 to numbers +C each number is a value representing one of the 4**length +C possible words of length length made up of 4 characters + SUBROUTINE ENCO(SEQ,IDIM,POSNS,CONST,LENGTH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM) + INTEGER POSNS(IDIM),CONST(LENGTH) + INTEGER CTONUM + EXTERNAL CTONUM + ISTART = 1 + DO 1 I=1,LENGTH + CONST(I) = 4**(I-1) + ISTART = ISTART - CONST(I) + 1 CONTINUE +C how many words of length length + IDIM1 = IDIM - (LENGTH-1) + DO 3 I=1,IDIM + POSNS(I) = 0 + 3 CONTINUE +C loop for each word + DO 100 I=1,IDIM1 + IP = I + N = ISTART + DO 30 J=1,LENGTH + IC = CTONUM(SEQ(IP)) + IF (IC.EQ.5) GO TO 100 + N = N + CONST(J) * IC + IP = IP + 1 +30 CONTINUE + POSNS(I) = N +100 CONTINUE + END + INTEGER FUNCTION IFROMC(CHARS,LENGTH,KBOUT) +C AUTHOR: RODGER STADEN +C INTEGER FUNCTION TO CONVERT CHARACTER STRINGS OF +C NUMERALS TO BINARY FORM + CHARACTER NUMBER*10,CHARS(LENGTH) +C LENGTH OF STRING NUMBER + LENS=10 + NUMBER=' ' + CALL RJSTFY(CHARS,NUMBER,LENS,LENGTH) + READ(NUMBER,1002,ERR=100)LIST +1002 FORMAT(I10) + IFROMC=LIST + RETURN +100 CONTINUE + WRITE(KBOUT,1006) +1006 FORMAT(' Error in internal read, value set to zero') + IFROMC=0 + RETURN + END + SUBROUTINE RJSTFY(ARRAY,STRING,LENS,LENGTH) +C AUTHOR: RODGER STADEN + CHARACTER STRING*(*),ARRAY(LENGTH) + STRING=' ' +C LOOK FOR FIRST NON SPACE CHAR + K=LENGTH+1 + DO 1 I=1,LENGTH + K=K-1 +1 IF(ARRAY(K).NE.' ')GO TO 2 +C ALL SPACES! + RETURN +2 CONTINUE + K1=K +C POINT TO RIGHT END OF STRING + K3=LENS+1 + DO 3 I=1,K1 + K3=K3-1 + STRING(K3:K3)=ARRAY(K) +3 K=K-1 + RETURN + END + SUBROUTINE OPENF(IDEV,FILNAM,IWRITE,IOK,KBIN,KBOUT) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*) + IOK=1 +10 CONTINUE + READ(KBIN,1000,ERR=300,END=300)FILNAM +1000 FORMAT(A) +C WANT TO WRITE? + IF(IWRITE.NE.0)GO TO 200 +C READONLY + CALL OPENRS(IDEV,FILNAM,IOK,LRECL,2) + RETURN +C WRITE +200 CONTINUE + CALL OPENRS(IDEV,FILNAM,IOK,LRECL,1) +300 CONTINUE + RETURN + END +C SQCOM + SUBROUTINE SQCOM(SEQ,IDIM) +C AUTHOR: RODGER STADEN + PARAMETER (MAXLST = 38) + CHARACTER SEQ(IDIM),LIST1(MAXLST),LIST2(MAXLST),TEMP + SAVE LIST1,LIST2 + DATA LIST1/ + +'C','T','A','G', + +'c','t','a','g', + +'D','V','B','H', + +'d','v','b','h', + +'K','L','M','N', + +'k','l','m','n', + +'R','Y','U', + +'r','y','u', + +'1','2','3','4', + +'5','6','7','8'/ + DATA LIST2/ + +'G','A','T','C', + +'g','a','t','c', + +'H','B','V','D', + +'h','b','v','d', + +'N','M','L','K', + +'n','m','l','k', + +'Y','R','A', + +'y','r','a', + +'4','3','2','1', + +'6','5','7','8'/ + DO 100 I=1,IDIM + TEMP = SEQ(I) + DO 50 J=1,MAXLST + IF(TEMP.EQ.LIST1(J))THEN + SEQ(I)=LIST2(J) + GO TO 99 + END IF +50 CONTINUE +99 CONTINUE +100 CONTINUE + END +C SQCOPY +C SEQUENCE COPYING PROGRAM + SUBROUTINE SQCOPY(SEQNCE,COMSEQ,IDIM) +C AUTHOR: RODGER STADEN + CHARACTER SEQNCE(IDIM),COMSEQ(IDIM) + DO 100 I=1,IDIM + COMSEQ(I)=SEQNCE(I) +100 CONTINUE + RETURN + END +C SQREV + SUBROUTINE SQREV(SEQNCE,IDIM) +C AUTHOR: RODGER STADEN + CHARACTER SEQNCE(IDIM),TEMP +C REVERSE THE SEQUENCE + IEND=IDIM/2 + DO 100 I=1,IEND + TEMP=SEQNCE(I) + SEQNCE(I)=SEQNCE(IDIM+1-I) + SEQNCE(IDIM+1-I)=TEMP +100 CONTINUE + RETURN + END +C DNARNA OR RNADNA + SUBROUTINE DNARNA(SEQ,IDSEQ) +C AUTHOR RODGER STADEN + CHARACTER SEQ(IDSEQ),TEMP,TCHAR,UCHAR,LTCHAR,LUCHAR + SAVE TCHAR,UCHAR,LTCHAR,LUCHAR + DATA TCHAR/'T'/,UCHAR/'U'/,LTCHAR/'t'/,LUCHAR/'u'/ + DO 10 I=1,IDSEQ + TEMP = SEQ(I) + IF(TEMP.EQ.TCHAR)THEN + SEQ(I)=UCHAR + ELSE IF(TEMP.EQ.LTCHAR)THEN + SEQ(I)=LUCHAR + ELSE IF(TEMP.EQ.UCHAR)THEN + SEQ(I)=TCHAR + ELSE IF(TEMP.EQ.LUCHAR)THEN + SEQ(I)=LTCHAR + END IF +10 CONTINUE + END + SUBROUTINE FMTDKN(IDEV,SEQNCE,IDIM) +C AUTHOR: RODGER STADEN + CHARACTER SEQNCE(IDIM) +C SET POINTERS TO FIRST AND LAST ELEMENTS ONE WRITE + JS=1 + JE=60 +10 CONTINUE +C SET JE TO LAST ELEMENT IF NECESSARY + IF(JE.GT.IDIM)JE=IDIM + WRITE(IDEV,1002)(SEQNCE(I),I=JS,JE) +1002 FORMAT(' ',60A1) +C TEST FOR END + IF(JE.NE.IDIM) THEN + JS=JE+1 + JE=JE+60 + GO TO 10 + END IF + END + SUBROUTINE FMTDK(IDEV,SEQNCE,IDIM) +C AUTHOR: RODGER STADEN + CHARACTER SEQNCE(IDIM) +C SET POINTERS TO FIRST AND LAST ELEMENTS ONE WRITE + JS=1 + JE=60 +10 CONTINUE +C SET JE TO LAST ELEMENT IF NECESSARY + IF(JE.GT.IDIM)JE=IDIM + WRITE(IDEV,1002)(SEQNCE(I),I=JS,JE) +1002 FORMAT(' ',60A1) +C TEST FOR END + IF(JE.EQ.IDIM)GO TO 20 +C INCREMENT FIRST AND LAST POINTERS + JS=JE+1 + JE=JE+60 + GO TO 10 +20 CONTINUE + CLOSE(UNIT=IDEV) + RETURN + END +C +C LWRAP + INTEGER FUNCTION LWRAP(IDIM,I) +C AUTHOR: RODGER STADEN +C TEST FOR END OF ARRAY,IFSO WRAP AROUND + LWRAP=I + IF(LWRAP.GT.IDIM)LWRAP=LWRAP-IDIM + RETURN + END + SUBROUTINE FMT4LN(SEQ1,SEQ2,MATCH,IDIM,ISW,ISX,IDEV) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM),SEQ2(IDIM),MATCH(IDIM) + INTEGER KL(6) + ISXX=ISX + ISWW=ISW + IE=0 +10 CONTINUE + IS=IE+1 + IE=IE+60 + IF(IE.GT.IDIM)IE=IDIM + N=IE-IS+1 + N=1+(N-1)/10 +C SET UP DECIMAL COUNTERS + DO 50 J=1,N + KL(J)=ISWW + ISWW=ISWW+10 +50 CONTINUE + WRITE(IDEV,1001)(KL(K),K=1,N) + WRITE(IDEV,1002)(SEQ1(K),K=IS,IE) + WRITE(IDEV,1002)(MATCH(K),K=IS,IE) + WRITE(IDEV,1002)(SEQ2(K),K=IS,IE) +1002 FORMAT( 10X,6(10A1,1X)) +C SET UP DECIMAL COUNTERS + DO 60 J=1,N + KL(J)=ISXX + ISXX=ISXX+10 +60 CONTINUE + WRITE(IDEV,1001)(KL(K),K=1,N) +1001 FORMAT( 5X,6(I6,5X)) + IF(IE.EQ.IDIM)RETURN + GO TO 10 + END + SUBROUTINE SQMTCH(SEQ1,SEQ2,MATCH,IDIM) +C AUTHOR: RODGER STADEN + CHARACTER SEQ1(IDIM),SEQ2(IDIM),MATCH(IDIM),BLANK,STAR + INTEGER CTONUM + EXTERNAL CTONUM + SAVE BLANK,STAR + DATA BLANK/' '/,STAR/'*'/ + CALL FILLC(MATCH,IDIM,BLANK) +C LOOK FOR MATCHES + DO 20 I=1,IDIM + IF(CTONUM(SEQ1(I)).EQ.CTONUM(SEQ2(I)))MATCH(I)=STAR +20 CONTINUE + RETURN + END + SUBROUTINE GETMRG(ISXMAX,ISYMAX,MARGL,MARGR,MARGB,MARGT,IMARG, + +IDEVM,FILNAM) +C AUTHOR: RODGER STADEN + INTEGER MARGB(IMARG),MARGT(IMARG),OPTION + CHARACTER FILNAM*(*) + DO 5 I=1,IMARG + MARGB(I)=0 + MARGT(I)=0 +5 CONTINUE +C OPEN(UNIT=IDEVM,FILE=FILNAM,STATUS='OLD',READONLY,ERR=4) + CALL OPENRS(IDEVM,FILNAM,IOK,LRECL,2) + IF(IOK.NE.0)GO TO 4 +C READ TITLE + READ(IDEVM,1002) +1002 FORMAT() + READ(IDEVM,1000,ERR=3)ISXMAX,ISYMAX + READ(IDEVM,1000,ERR=3)MARGL,MARGR +1000 FORMAT(2I6) +1 CONTINUE + READ(IDEVM,1001,ERR=3,END=2)OPTION,M1,M2 +1001 FORMAT(3I6) + IF(OPTION.LE.IMARG)THEN + MARGB(OPTION)=M1 + MARGT(OPTION)=M2 + END IF + GO TO 1 +2 CONTINUE + CLOSE(UNIT=IDEVM) + RETURN +3 CONTINUE + WRITE(*,*)' Error in margin file' + CLOSE(UNIT=IDEVM) + RETURN +4 CONTINUE + WRITE(*,*)' Error opening margin file' + CLOSE(UNIT=IDEVM) + RETURN + END + SUBROUTINE MOVEC(SEQ,IDIMX,IDIM,IPOS,NCHAR1) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIMX) + INTEGER TO,FROM + NCHAR=ABS(NCHAR1) +C LEFT OR RIGHT? + IF(NCHAR1.LT.0)GO TO 20 +C RIGHT + FROM=IDIM + TO=IDIM+NCHAR +C NUMBER TO MOVE? + NUM=IDIM-IPOS+1 + DO 10 I=1,NUM + SEQ(TO)=SEQ(FROM) + TO=TO-1 + FROM=FROM-1 +10 CONTINUE + RETURN +20 CONTINUE +C LEFT + FROM=IPOS+NCHAR + TO=IPOS +C NUMBER TO MOVE? + NUM=IDIM-FROM+1 + DO 30 I=1,NUM + SEQ(TO)=SEQ(FROM) + TO=TO+1 + FROM=FROM+1 +30 CONTINUE + RETURN + END + CHARACTER*(*) FUNCTION ATOS(ARRAY,IDIM) +C AUTHOR RODGER STADEN + CHARACTER ARRAY(IDIM) +C FUNCTION TO CONVERT A CHARACTER ARRAY INTO A CHARACTER STRING + ATOS=' ' + DO 10 I=1,IDIM + ATOS(I:I)=ARRAY(I) +10 CONTINUE + RETURN + END + SUBROUTINE FILLC(SEQ,IDIM,CH) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDIM),CH + DO 10 I=1,IDIM + SEQ(I) = CH +10 CONTINUE + RETURN + END +C FILLI + SUBROUTINE FILLI(SEQ,IDIM,CH) +C AUTHOR: RODGER STADEN + INTEGER SEQ(IDIM),CH + DO 10 I=1,IDIM + SEQ(I) = CH +10 CONTINUE + RETURN + END + SUBROUTINE FILLR(ARRAY,IDIM,CH) +C AUTHOR RODGER STADEN + REAL ARRAY(IDIM),CH +C ROUTINE TO FILL REAL ARRAY WITH VALUE CHAR + DO 10 I = 1 , IDIM + ARRAY(I) = CH +10 CONTINUE + END + SUBROUTINE GETMAT(IDEV,FILNAM,MATRIX,IDM,CHRSET,KBOUT,IOK) +C AUTHOR RODGER STADEN + INTEGER MATRIX(IDM,IDM) + CHARACTER FILNAM*(*),CHRSET(IDM) + CALL OPENRS(IDEV,FILNAM,IOK,LRECL,2) + IF(IOK.NE.0)THEN + WRITE(KBOUT,*)'Error opening score matrix file' + RETURN + END IF + READ(IDEV,1000) + DO 6 I=1,IDM + READ(IDEV,1000,ERR=100)CHRSET(I),(MATRIX(I,K),K=1,IDM) +1000 FORMAT(A1,26I3) +6 CONTINUE + CLOSE(UNIT=IDEV) + RETURN +100 CONTINUE + WRITE(KBOUT,*)'Error reading score matrix file' + CLOSE(UNIT=IDEV) + IOK = 1 + END +C + SUBROUTINE REDCOD(SUM,IDEV) +C AUTHOR: RODGER STADEN + REAL SUM(4,4,4) + READ(IDEV,1000) +1000 FORMAT( ) + DO 10 I=1,4 + DO 20 K=1,4 +20 READ(IDEV,1001,ERR=30,END=30)(SUM(I,J,K),J=1,4) +10 READ(IDEV,1000,ERR=30,END=30) +1001 FORMAT(5X,4(6X,F5.0)) + RETURN +30 CONTINUE + WRITE(*,*)' Error reading codon table file' + END +C + SUBROUTINE WRTCOD(SUM,IDEV,PAA) +C AUTHOR: RODGER STADEN + REAL SUM(4,4,4) + CHARACTER BASE(4),PAA(5,5,5) + SAVE BASE + DATA BASE/'T','C','A','G'/ +C + WRITE(IDEV,1001) +1001 FORMAT(6X,'===========================================') + DO 10 I=1,4 + DO 20 K=1,4 + WRITE(IDEV,1000)(PAA(K,J,I), + +BASE(I),BASE(J),BASE(K),SUM(I,J,K),J=1,4) +20 CONTINUE +10 WRITE(IDEV,1001) +1000 FORMAT(5X,4(1X,A1,1X,3A1,F5.0)) + END + INTEGER FUNCTION NORP(SEQ,IDSEQ) +C AUTHOR RODGER STADEN + CHARACTER SEQ(IDSEQ),CHAR1,DNAC*5 + CHARACTER TUPPER + SAVE DNAC + EXTERNAL TUPPER + DATA DNAC/'ATCGU'/ +C RETURNS NORP = 5 IF >85% A,C,G,T,U ELSE NORP = 26 + DNA = 0. + DO 10 I = 1,IDSEQ + CHAR1 = TUPPER(SEQ(I)) + DO 5 J=1,5 + IF(CHAR1.EQ.DNAC(J:J)) THEN + DNA = DNA + 1. + GO TO 10 + END IF + 5 CONTINUE +10 CONTINUE + X = DNA/REAL(IDSEQ) + N = 26 + IF(X.GT.0.85) N = 5 + NORP = N + END + SUBROUTINE COPYI(IN,OUT,ID) +C AUTHOR RODGER STADEN + INTEGER IN(ID),OUT(ID) +C COPY INTEGER ARRAY IN TO ARRAY OUT + DO 10 I = 1, ID + OUT(I) = IN(I) +10 CONTINUE + END + SUBROUTINE CONNUM(SEQIN,SEQOUT,IDIM) +C AUTHOR: RODGER STADEN + CHARACTER SEQIN(IDIM) + INTEGER SEQOUT(IDIM) + INTEGER CTONUM + EXTERNAL CTONUM + DO 10 I=1,IDIM + SEQOUT(I)=CTONUM(SEQIN(I)) +10 CONTINUE + RETURN + END + SUBROUTINE CONNUN(SEQIN,SEQOUT,IDIM) +C AUTHOR: RODGER STADEN + CHARACTER SEQIN(IDIM) + INTEGER SEQOUT(IDIM) + INTEGER DTONUM + EXTERNAL DTONUM + DO 10 I=1,IDIM + SEQOUT(I) = DTONUM(SEQIN(I)) +10 CONTINUE + END + INTEGER FUNCTION NMMTCH(S1,S2) +C AUTHOR RODGER STADEN +C RETURNS 0 = MATCH, 1 = NO MATCH +C Blank names do not match ie all spaces + CHARACTER S1*(*),S2*(*) + NMMTCH = 1 + L1 = LEN(S1) + L2 = LEN(S2) + L1S = INDEX(S1,' ') + L2S = INDEX(S2,' ') + IF(L1S.GT.1) L1 = L1S - 1 + IF(L2S.GT.1) L2 = L2S - 1 + IF(L1.NE.L2) RETURN + CALL CCASE(S1,2) + CALL CCASE(S2,2) + IF(S1(1:L1).NE.S2(1:L1)) RETURN + NMMTCH = 0 + END + INTEGER FUNCTION ITOSL(LINE,N) +C Sent int N return its character string left justified +C and the length of the string. Length 0 means error. + CHARACTER STRING*10,LINE*(*) + ITOSL = 0 + WRITE(STRING,1000,ERR=100)N +1000 FORMAT(I10) + CALL LJST(STRING) + I = INDEX(STRING,' ') - 1 + LINE(1:I) = STRING(1:I) + ITOSL = I +100 CONTINUE + END + SUBROUTINE LJST(STRING) +C Left justify a string, fill rest with spaces + CHARACTER STRING*(*) + EXTERNAL NOTILR + L = LEN(STRING) + I = NOTILR(STRING,L,' ') + IF(I.NE.1) THEN + J = 1 + DO 10 K = I,L + STRING(J:J) = STRING(K:K) + J = J + 1 +10 CONTINUE + STRING(J:L) = ' ' + END IF + END + SUBROUTINE DIVR(S,N,D) +C Divide array S of N elements by D + REAL S(N) + IF(D.NE.0.) THEN + DO 10 I=1,N + S(I) = S(I)/D +10 CONTINUE + END IF + END + REAL FUNCTION SUMR(R,N) +C Sum the N elements of array R + REAL R(N) + S = 0. + DO 10 I = 1,N + S = S + R(I) +10 CONTINUE + SUMR = S + END + LOGICAL FUNCTION NUMBER(CHR) +C Return true if CHR is a digit + CHARACTER CHR,NUMS*10 + SAVE NUMS + DATA NUMS/'0123456789'/ + NUMBER = .FALSE. + IF(INDEX(NUMS,CHR).NE.0) NUMBER = .TRUE. + END + INTEGER FUNCTION LASTN(LINEIN,K) +C Return position of last digit (moving left to right) in LINEIN + CHARACTER LINEIN*(*) + LOGICAL NUMBER + EXTERNAL NUMBER + LASTN = 0 + DO 10 I = K,LEN(LINEIN) +C CALL OUTPT(LINEIN)(I:I) + IF(.NOT.(NUMBER(LINEIN(I:I)))) RETURN + LASTN = I +10 CONTINUE + END + INTEGER FUNCTION FIRSTN(LINEIN,K) +C Return position of first digit (moving left to right) in LINEIN + CHARACTER LINEIN*(*) + LOGICAL NUMBER + EXTERNAL NUMBER + DO 10 I = K,LEN(LINEIN) +C CALL OUTPT(LINEIN)(I:I) + FIRSTN = I + IF(NUMBER(LINEIN(I:I))) RETURN +10 CONTINUE + FIRSTN = 0 + END + LOGICAL FUNCTION ONEOF(CHRS,CHR) +C Return true if CHR is in set of chars stored in CHRS + CHARACTER CHR,CHRS*(*) + ONEOF = .FALSE. + IF(INDEX(CHRS,CHR).NE.0) ONEOF = .TRUE. + END + LOGICAL FUNCTION STRNGM(S1,S2) +C Return true if the first L chars of S1 and S2 match +C L is the length of the shortest string. Independent of case. + CHARACTER S1*(*),S2*(*),TUPPER + EXTERNAL TUPPER + L = MIN(LEN(S1),LEN(S2)) + STRNGM = .FALSE. + DO 10 I = 1,L + IF(TUPPER(S1(I:I)).NE.TUPPER(S2(I:I))) RETURN +10 CONTINUE + STRNGM = .TRUE. + END +C OPENF1 +C IWRITE NE 0 MEANS WRITE NEW FILE, ELSE MEANS READONLY + SUBROUTINE OPENF1(IDEV,FILNAM,IWRITE,IOK,KBIN,KBOUT, + +PROMPT, + +IHELPS,IHELPE,HELPF,IDEVH) +C AUTHOR: RODGER STADEN + CHARACTER FILNAM*(*),HELPF*(*),PROMPT*(*) + PARAMETER (MAXPRM = 16, MAXNAM = 60) + CHARACTER PERR(2)*(MAXPRM),NEWFN*(MAXNAM) + INTEGER ANS,DELF + EXTERNAL DELF,NOTRL + IOK=1 + ICOUNT = 0 +C + LENGTH = NOTRL(FILNAM,LEN(FILNAM),' ') + IF(IWRITE.EQ.1) LENGTH = 0 + LIN = LENGTH +10 CONTINUE + IF(ICOUNT.EQ.5) THEN + IOK = 1 + RETURN + END IF + ICOUNT = ICOUNT + 1 + CALL GTSTR(PROMPT,FILNAM,NEWFN,LENGTH,KBOUT,KBIN,K) + IF(K.EQ.1) THEN + CALL HELP2(IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + ICOUNT = 0 + GO TO 10 + END IF + IF(K.EQ.2) THEN + IOK = 2 + RETURN + END IF +C if no default and blank line try again + IF((K.EQ.3).AND.(LIN.EQ.0)) GO TO 10 + IF(K.EQ.4) GO TO 10 + IF(LENGTH.GT.0) FILNAM = NEWFN + IF(IWRITE.EQ.0) THEN +C OLD FILE (I.E. READ ONLY) + CALL OPENRS(IDEV,FILNAM,IOK,LRECL,2) + IF(IOK.EQ.0) RETURN +C problem opening file + IF(IOK.EQ.3) CALL ERROM(KBOUT,'File not found') + CALL YESNO(ANS,'Retype file name', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANS.EQ.0) GO TO 10 + RETURN + ELSE IF(IWRITE.EQ.1) THEN +C NEW FILE (I.E. WRITE) + 20 CONTINUE + CALL OPENRS(IDEV,FILNAM,IOK,LRECL,1) + IF(IOK.EQ.0) RETURN +C problem opening file + IF(IOK.EQ.2) THEN + CALL ERROM(KBOUT,'File already exists') + PERR(1) = 'Retype file name' + PERR(2) = 'Replace file' + IDO = 1 + CALL RADION('Select action',PERR,2,IDO, + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(IDO.LT.1) RETURN + IF(IDO.EQ.1) GO TO 10 + IF(IDO.EQ.2) THEN + IOK = DELF(FILNAM,IDEV,JRECL,2) + IF(IOK.EQ.0) GO TO 20 + CALL ERROM(KBOUT,'File delete failed') + RETURN + END IF + ELSE + CALL ERROM(KBOUT,'File opening failed') + CALL YESNO(ANS,'Retype file name', + + IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + IF(ANS.EQ.0) GO TO 10 + RETURN + END IF + END IF + END + INTEGER FUNCTION GNFFOF(IDEV,NAME) + CHARACTER NAME*(*) + EXTERNAL NOTLR +C +C routine to read a file of file names and return a name +C deals with leading spaces and trims names at first space +C after name: eg ' fred is a bum' is returned as 'fred' +C needed because file names can contain spaces (not our file names!) +C and the open statement expects the names to match precisely +C +C return 0 = ok, 2 = empty line in file, 3 = error in read, 1 = end of file +C + READ(IDEV,1000,ERR=100,END=200)NAME + 1000 FORMAT(A) +C +C get first non space position +C + LENGTH = LEN(NAME) + I = NOTLR(NAME,LENGTH,' ') +C empty line ? + IF(I.EQ.0) THEN + GNFFOF = 2 + RETURN + END IF +C now want first space after I + J = INDEX(NAME(I+1:),' ') + IF(J.EQ.0) THEN + J = LENGTH + ELSE + J = J + I - 1 + END IF + CALL SHFTLS(NAME,I,1,J) + NAME(J-I+2:) = ' ' + GNFFOF = 0 + RETURN + 100 CONTINUE + GNFFOF = 3 + RETURN + 200 CONTINUE + GNFFOF = 1 + END + SUBROUTINE WRITFF(IDEV,SEQ,IDSEQ,ENAME,TITLE) +C AUTHOR: RODGER STADEN + CHARACTER SEQ(IDSEQ),NL,ENAME*(*),TITLE*(*) + NL = CHAR(10) + CALL WRITEB('>',1,IDEV) + CALL WRITEB(ENAME,LEN(ENAME),IDEV) + CALL WRITEB(' ',1,IDEV) + CALL WRITEB(TITLE,LEN(TITLE),IDEV) + CALL WRITEB(NL,1,IDEV) + JS=1 + JE=60 +10 CONTINUE + IF (JE.GT.IDSEQ) JE = IDSEQ + DO 5 I=JS,JE + CALL WRITEB(SEQ(I),1,IDEV) + 5 CONTINUE + CALL WRITEB(NL,1,IDEV) + IF(JE.NE.IDSEQ) THEN + JS=JE+1 + JE=JE+60 + GO TO 10 + END IF + END + SUBROUTINE SHFTLS(STRING,FROMS,TO,FROME) + CHARACTER STRING*(*) + INTEGER FROMS,TO,FROME +C +C shift a string left from froms to to +C + J = TO + DO 10 I=FROMS,FROME + STRING(J:J) = STRING(I:I) + J = J + 1 + 10 CONTINUE + END diff --git a/src/staden/summar.f b/src/staden/summar.f new file mode 100644 index 0000000..94c78b0 --- /dev/null +++ b/src/staden/summar.f @@ -0,0 +1,132 @@ + SUBROUTINE SUMMAR(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +SEQ1,IDIM1,GEL,LREG,RREG,IGELC,PERCD,IDBSIZ,CHARS, + +ID1,CHRSIZ,MAXGL2,IDEVW,MAXGEL,LINOU1,LINOU2,MXGOOD) +C AUTHOR: RODGER STADEN + INTEGER RELPG(IDBSIZ),CHRSIZ + INTEGER LNGTHG(IDBSIZ),LNBR(IDBSIZ),RNBR(IDBSIZ) + CHARACTER SEQ1(IDIM1) + CHARACTER GEL(MAXGEL) + INTEGER LREG,RREG,LSEQNO,POSN,Y,POSN1 + INTEGER GELC + CHARACTER LINOU1(MAXGEL),LINOU2(MAXGEL),GTCONC + INTEGER CHARS(CHRSIZ,ID1,MAXGL2) + EXTERNAL INDEXS,LWRAPS,GTCONC +C 28-7-91 added extra parameter: mxgood is the maximum reading +C length for which we have confidence, so only the parts of +C reads 1 to mxgood will be included in the calculation +C SET INITIAL VALUES +C hard to understand this very old code! rewrite it. +C we have a summing array of twice the length of the longest sequence +C posn is posn in contig of next to write, lseqno is current posn in contig +C we write when lseqno-posn ge the length of the longest seq + POSN=LREG + GELC=IGELC + LINLEN=MAXGEL + LSEQNO=RELPG(GELC) + IEND=0 + DO 40 I=1,MAXGL2 + DO 40 J=1,ID1 + DO 40 K=1,CHRSIZ + CHARS(K,J,I)=0 +40 CONTINUE +50 CONTINUE + ISS=1 + IF(LNGTHG(GELC).LT.0)ISS=2 + CALL READW(IDEVW,GELC,GEL,MAXGEL) +C LOOP FOR RELEVANT ELEMENTS THIS GEL +C only use mxgood characters from start of read +C + IF(ISS.EQ.1) THEN + N = MIN(MXGOOD,ABS(LNGTHG(GELC))) + IF(LSEQNO.LT.LREG)LSEQNO=LREG + IS = LSEQNO-RELPG(GELC)+1 + ELSE +C LOOP FOR RELEVANT ELEMENTS THIS GEL +C only use mxgood characters from start of read (right end for these) +C + N = ABS(LNGTHG(GELC)) + IS = MAX(1,(ABS(LNGTHG(GELC)) - MXGOOD + 1)) + LSEQNO = RELPG(GELC) + MAX(0,ABS(LNGTHG(GELC)) - MXGOOD) + IF(LSEQNO.LT.LREG)LSEQNO=LREG + END IF + DO 70 I=IS,N + JJ = INDEXS(GEL(I),JSCORE) + JJJ = LWRAPS(LSEQNO,MAXGL2) + CHARS(JJ,ISS,JJJ) = CHARS(JJ,ISS,JJJ) + JSCORE + LSEQNO = LSEQNO + 1 +70 CONTINUE + IF(RNBR(GELC).EQ.0)GO TO 200 + GELC=RNBR(GELC) + LSEQNO=RELPG(GELC) + IF(LSEQNO.GT.RREG)GO TO 200 +C ENOUGH TO OUTPUT? + Y=LSEQNO-POSN + IF(Y.GE.MAXGEL)GO TO 210 + GO TO 50 +200 CONTINUE +C SET FLAG TO SHOW END REACHED + IEND=1 + LINLEN=MAXGEL + Y=RREG-POSN + IF(Y.LT.MAXGEL)LINLEN=Y+1 +210 CONTINUE +C SET POINTER TO SEQ1 + POSN1=POSN-1 +C PREPARE NEXT SECTION OF CHARS FOR OUTPUT + DO 230 I=1,LINLEN + JJJ = LWRAPS(POSN,MAXGL2) + LINOU1(I) = GTCONC(CHARS(1,1,JJJ),CHRSIZ,PERCD) + LINOU2(I) = GTCONC(CHARS(1,2,JJJ),CHRSIZ,PERCD) + DO 250 J=1,CHRSIZ + CHARS(J,1,JJJ)=0 + CHARS(J,2,JJJ)=0 +250 CONTINUE + POSN=POSN+1 +230 CONTINUE +C +C COMPARE STRANDS +C + DO 500 I=1,LINLEN +C WRITE(*,*)I,LINOU1(I),LINOU2(I) + POSN1=POSN1+1 + IF(LINOU1(I).EQ.LINOU2(I)) THEN + IF(LINOU1(I).EQ.'-') THEN + SEQ1(POSN1) = '3' + GO TO 500 + END IF + IF(LINOU1(I).EQ.'*') THEN + SEQ1(POSN1) = '3' + GO TO 500 + END IF + SEQ1(POSN1) = '0' + ELSE + IF((LINOU1(I).EQ.'*').AND.(LINOU2(I).EQ.'-')) THEN + SEQ1(POSN1) = '3' + GO TO 500 + END IF + IF((LINOU2(I).EQ.'*').AND.(LINOU1(I).EQ.'-')) THEN + SEQ1(POSN1) = '3' + GO TO 500 + END IF + IF((LINOU1(I).NE.'-').AND.(LINOU1(I).NE.'*')) THEN + SEQ1(POSN1) = '1' + IF((LINOU2(I).NE.'-').AND.(LINOU2(I).NE.'*')) + + SEQ1(POSN1) = '4' + GO TO 500 + END IF + IF((LINOU2(I).NE.'-').AND.(LINOU2(I).NE.'*')) THEN + SEQ1(POSN1) = '2' + IF((LINOU1(I).NE.'-').AND.(LINOU1(I).NE.'*')) + + SEQ1(POSN1) = '4' + GO TO 500 + END IF + END IF +500 CONTINUE + IF(POSN.GT.RREG)RETURN + IF((IEND.EQ.1).AND.(POSN.LE.RREG))GO TO 200 +C ANY MORE MAXGEL CHAR LENGTHS TO OUTPUT + Y=LSEQNO-POSN + IF(Y.LT.MAXGEL)GO TO 50 +C FINISHED COMPLETELY? + GO TO 210 + END diff --git a/src/staden/tagEditor.c b/src/staden/tagEditor.c new file mode 100644 index 0000000..812d4e3 --- /dev/null +++ b/src/staden/tagEditor.c @@ -0,0 +1,312 @@ +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include +#include + +#include "contigEditor.h" +#include "tagUtils.h" +#include "tagdb.h" + +#define TEXT_START ((XawTextPosition)0) +#define TEXT_END ((XawTextPosition)32767) + +/* ---- Private Variables ---- */ +static Widget tagEditorShellWid; +static Widget tag_type_wid; +static Widget tag_text_wid; +static int source_changed; +static int use_default; +static int cur_tag_index; +static int tagEditorIsUp; /* popup state of editor */ +static int tagEditorAbort; /* abort status when exiting */ + +/* ---- Private Procedures ---- */ + +static void quitCallback(Widget w, XtPointer client_data, XtPointer call_data) +{ + XtPopdown(tagEditorShellWid); + tagEditorIsUp = 0; + tagEditorAbort = 1; + +} +static void leaveCallback(Widget w, XtPointer client_data, XtPointer call_data) +{ + XtPopdown(tagEditorShellWid); + tagEditorIsUp = 0; + tagEditorAbort = 0; +} + +static void SourceChanged(Widget w, XtPointer junk, XtPointer garbage) +{ + XtRemoveCallback(w, XtNcallback, SourceChanged, NULL); + source_changed = TRUE; +} + +void ResetSourceChanged(Widget widget) +{ + XtAddCallback(XawTextGetSource(widget), XtNcallback, SourceChanged, NULL); + source_changed = FALSE; +} + + +void setButtonName(Widget w, char *c) +{ + Arg args[10]; + int nargs; + char buttonName[255]; + + /* set default tag type */ + sprintf(buttonName,"Type: %s",c); + nargs = 0; + XtSetArg(args[nargs], XtNlabel, buttonName); nargs++; + XtSetValues(w, args, nargs); +} + +static void setDefaultText(char *s) +{ + XawTextBlock block; + + block.firstPos = 0; + block.length = (s==NULL)?0:(int)strlen(s); + block.ptr = s; + block.format = FMT8BIT; + + XawTextReplace(tag_text_wid,TEXT_START,TEXT_END,&block); + XawTextSetInsertionPoint(tag_text_wid,TEXT_END); + +} + +int idToIndex(char *id) +{ + int i; + if (id==NULL) return 0; + for (i=0; itype); + cur_tag_index = idToIndex(t->id); + + if (!source_changed && use_default) { + setDefaultText(t->default_text); + ResetSourceChanged(tag_text_wid); + } +} + +static void readInTagDB() +{ +#define TAGDB "TAGDB" + char *filename; + /* struct stat statBuff; */ + + /* check trace file exists */ + if ((filename = (char *) getenv(TAGDB))!=NULL) + parse(filename); + else + parse(TAGDB); + + /* + if ( stat(TAGDB,&statBuff) < 0 ) + parse(TAGDB); + else { + filename = getenv(TAGDB); + parse(filename); + } + */ +} + +static Pixel ColourNameToPixel(Widget w, String c) +{ + XColor rgb_db_def, hardware_def; + Colormap cmap; + Status s; + + cmap = DefaultColormap(XtDisplay(w),DefaultScreen(XtDisplay(w))); + s = XAllocNamedColor(XtDisplay(w), cmap, c, &rgb_db_def, &hardware_def); + + return hardware_def.pixel; +} + + +static void setUpColourMap(Widget w) +{ + int i; + + for (i=0;i +static tagStruct *tagFreeList = NULL; +/* tagUtils.c */ +/* define external routines */ + + +/* low level */ + +static void replace_comment(comment_id cp, char *c) +{ + delete_comment(cp); + (void) put_comment(c); + /* a bit dodgy here. Should work ok with this data structure though */ +} + +void force_comment(tagStruct *t) +/* +** Force comment to be in memory +*/ +{ + if (!(t->flags & TAG_COMMENT_IN_MEMORY)) { + /* + ** Read in from database + */ + if (t->tagrec.comment) { + t->newcomment = get_comment(t->tagrec.comment); + t->newcommentlen = (int)strlen(t->newcomment); + } else { + t->newcomment = (char *) TAG_MALLOC(1); + t->newcomment[0] = '\0'; + t->newcommentlen = 0; + } + t->flags |= TAG_COMMENT_IN_MEMORY; + } +} + + +/* +** Tag internal memory management routines +*/ +tagStruct *newTag() +{ + tagStruct *t; + if (tagFreeList == NULL) { + t = (tagStruct *) TAG_MALLOC(sizeof(tagStruct)); + } else { + t = tagFreeList; + tagFreeList = t->next; + } + + /* + ** Null all the fields + */ + t->tagrec.position = 0; + t->tagrec.length = 0; + t->tagrec.comment = 0; + t->tagrec.type.i = 0x20202020; + t->tagrec.next = 0; + t->original_tag_id = 0; + t->newcomment = NULL; + t->newcommentlen = 0; + t->flags = TAG_UNCHANGED; + t->next = NULL; + t->time = 0; + + return t; +} + +static void freeTag(tagStruct* t) +{ + TAG_FREE(t->newcomment); + t->newcommentlen = 0; + t->next = tagFreeList; + tagFreeList = t; +} + +tagStruct *readTagList(int seq) +{ + tagStruct *s,*t,*u; + s=t=newTag(); + (void) read_tag(seq, &t->tagrec); + t->original_tag_id = seq; + while (t->tagrec.next) { + u=newTag(); + read_tag(t->tagrec.next,&u->tagrec); + u->original_tag_id = t->tagrec.next; + t->next = u; + t=u; + } + t->next = NULL; + + return s; +} + +void destroyTagList(tagStruct *s) +{ + tagStruct *t,*u; + + t=s; + while (t!=NULL) { + u=t->next; + freeTag(t); + t=u; + } +} + +/* +** Tag creation and modification +*/ +void insertTag(EdStruct *xx, int seq, tagStruct *t) +/* +** insert tag, sorting by position +*/ +{ + tagStruct *u, *v; + + u = (tagStruct *) DBgetTags(xx,seq); + v = NULL; + while (u != NULL && + (u->tagrec.position <= t->tagrec.position || u->flags & TAG_DELETED) ) { + v = u; + u = u->next; + } + t->next = u; + if (v != NULL) { + v->next = t; + } else { + DBsetTags(xx,seq,t); + } + +} + +void createTag(EdStruct *xx) +{ + int seq,start,length; + char *newcomment; + char newtype[4]; + int aborted; + tagStruct *t; + + if (! getSelection(xx, &seq, &start, &length, &t)) { + /* default selection is current cursor position */ + seq = xx->cursorSeq; + start = xx->cursorPos; + length = 1; + } + /* don't invoke Tag editor for consensus */ + if (seq) { + aborted = invokeTagEditor("",newtype,"",&newcomment); + if (! aborted ) { + /* + ** Create a new tag and insert it with comment into bimbo falix + */ + t = newTag(); + t->flags = TAG_INSERTED | + TAG_LENGTH_CHANGED | + TAG_POSITION_CHANGED | + TAG_TYPE_CHANGED; + t->tagrec.position = normalisePos(xx,seq,start,length); + t->tagrec.length = length; + strncpy(t->tagrec.type.c,newtype,4); + if (newcomment != NULL) + t->flags |= TAG_COMMENT_CHANGED | + TAG_COMMENT_IN_MEMORY; + t->newcomment = newcomment; + t->newcommentlen = (int)strlen(newcomment); + insertTag(xx,seq,t); + redisplaySequences (xx,xx->namesWid, xx->sequencesWid, xx->displayPos, xx->displayWidth); + xx->select_tag = t; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } + } +} + +void editTag(EdStruct *xx) +{ + int seq,start,length; + char *newcomment; + char *oldcomment; + tag_types newtype; + int aborted; + tagStruct *t; + + if (! getSelection(xx, &seq, &start, &length, &t)) { + /* default selection is current cursor position */ + seq = xx->cursorSeq; + start = xx->cursorPos; + t = findTag(xx,seq,start); + _select_tag(xx,seq,t); + (void) getSelection(xx, &seq, &start, &length, &t); + } else if (t==NULL) { + t = findTag(xx,seq,start); + _select_tag(xx,seq,t); + (void) getSelection(xx, &seq, &start, &length, &t); + } + if (t==NULL) return; + + /* + ** Find current comment + */ + /* + if (t->flags & TAG_COMMENT_IN_MEMORY) { + oldcomment = t->newcomment; + } + else { + .* + ** Read in from database + *. + if (t->tagrec.comment) { + oldcomment = get_comment(t->tagrec.comment); + t->flags |= TAG_COMMENT_IN_MEMORY; + } else { + oldcomment = NULL; + } + } + */ + force_comment(t); + oldcomment = t->newcomment; + + /* + ** Edit + */ + aborted = invokeTagEditor(t->tagrec.type.c,newtype.c,oldcomment,&newcomment); + if (! aborted ) { + /* + ** Update components of tag that have changed + */ + /* + ** Don't update position!!! + t->tagrec.position = start; + t->tagrec.length = length; + */ + /* + ** Check for new type + */ + if (t->tagrec.type.i != newtype.i) { + t->tagrec.type.i = newtype.i; + t->flags |= TAG_TYPE_CHANGED; + } + /* + ** Check comment is something new + */ + if (newcomment != NULL) { + t->flags |= TAG_COMMENT_CHANGED | TAG_COMMENT_IN_MEMORY; + /* + ** throw old comment away + */ + TAG_FREE(oldcomment); + t->newcomment = newcomment; + t->newcommentlen = (int)strlen(newcomment); + } + + redisplaySequences (xx,xx->namesWid, xx->sequencesWid, xx->displayPos, xx->displayWidth); + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } + +} + +void _delete_tag(EdStruct *xx, int seq, tagStruct *t) +/* +** Delete tag t from data structure +*/ +{ + /* + ** Physical removal by delinking + */ + if (t->flags & TAG_INSERTED) { + tagStruct *u, *v; + u = (tagStruct *) DBgetTags(xx,seq); + v = NULL; + while (u != NULL && u != t) { + v = u; + u = u->next; + } + if (u==NULL) return; /* not found */ + if (v==NULL) + DBsetTags(xx,seq,u->next); + else + v->next = u->next; + freeTag(u); + u->flags |= TAG_DELETED; + } else + t->flags |= TAG_DELETED; + + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + +} + +void deleteTag(EdStruct *xx) +/* +** A rather brutal delete +*/ +{ + int seq,start,length; + tagStruct *t; + + if (! getSelection(xx, &seq, &start, &length, &t)) { + /* default selection is current cursor position */ + seq = xx->cursorSeq; + start = xx->cursorPos; + t = findTag(xx,seq,start); + _select_tag(xx,seq,t); + (void) getSelection(xx, &seq, &start, &length, &t); + } else if (t==NULL) { + t = findTag(xx,seq,start); + _select_tag(xx,seq,t); + (void) getSelection(xx, &seq, &start, &length, &t); + } + if (t==NULL) return; + + _delete_tag(xx,seq,t); + redisplaySequences (xx,xx->namesWid, xx->sequencesWid, xx->displayPos, xx->displayWidth); + +} + +void getTagSplodge(EdStruct *xx, int seq, int pos, int width, XawSheetInk *ink) +/* +** get the hilighting of a sequence from its `pos' base for `width' bases +** Bases number from 0? +*/ +{ + + int i; + tagStruct *t; + int npos,tpos; + + + if (xx->reveal_cutoffs) { + int length = DBgetLength(xx,seq); + + /*blank start*/ + for (i=0; inext; + + while (t != NULL && + (t->tagrec.position < npos+width || t->flags & TAG_DELETED) ) { + if (!(t->flags & TAG_DELETED) && + t->tagrec.position+t->tagrec.length > npos && + t->tagrec.type.c[0] != '*') { + int l,r; + int db=idToIndex(t->tagrec.type.c); + tpos = normalisePos(xx,seq,t->tagrec.position,t->tagrec.length); + if (tpos < pos) + l=0; + else + l=tpos-pos; + if (tpos + t->tagrec.length > pos+width) + r=width; + else + r=tpos-pos + t->tagrec.length; + for (i=l;inext; + } + +} + +static void tagEditDelete(EdStruct *xx, int seq, int pos, char deletedBase) +{ + tagStruct *t; + + t = (tagStruct *) DBgetTags(xx,seq); + while (t != NULL && + (t->tagrec.position <= pos || t->flags & TAG_DELETED) ) { + if( !(t->flags & TAG_DELETED) && + t->tagrec.position==pos && + strncmp(t->tagrec.type.c,TAG_TYPE_INSERT,3)==0){ + _delete_tag(xx, seq, t); + return; + } + t = t->next; + } + + /* + ** We didn't find a tag so create a delete one + */ + t = newTag(); + t->flags = TAG_INSERTED | + TAG_LENGTH_CHANGED | + TAG_POSITION_CHANGED | + TAG_TYPE_CHANGED; + t->tagrec.position = pos; + t->tagrec.length = 0; + strncpy(t->tagrec.type.c,TAG_TYPE_DELETE,4); + + /* + ** Stash the deleted base somewhere + */ + t->tagrec.type.c[3] = deletedBase; + + insertTag(xx,seq,t); + +} + +static void tagEditInsert(EdStruct *xx, int seq, int pos, char insertedBase) +{ + tagStruct *t; + /* + ** Create an new insert tag here + */ + t = newTag(); + t->flags = TAG_INSERTED | + TAG_LENGTH_CHANGED | + TAG_POSITION_CHANGED | + TAG_TYPE_CHANGED; + t->tagrec.position = pos; + t->tagrec.length = 1; + strncpy(t->tagrec.type.c,TAG_TYPE_INSERT,3); + + /* + ** Stash the deleted base somewhere + */ + t->tagrec.type.c[3] = insertedBase; + + insertTag(xx,seq,t); +} + + +char normaliseBase(EdStruct *xx,int seq,char deletedBase) +{ + + if (DBgetComp(xx,seq) == COMPLEMENTED) { + char base = deletedBase; + int_f i=1; + sqcom_(&base,&i,(int_fl)1); + return base; + } else + return deletedBase; +} + + +void tagInsertBase(EdStruct *xx,int seq,int pos,char *insertedBase) +/* +** A character has been inserted at position `pos' in sequence `seq' +** Adjust tag positions and lengths accordingly +*/ +{ + int npos = normalisePos(xx,seq,pos,1); + char base = normaliseBase(xx,seq,*insertedBase); + + tagStruct *t,*del_tag; + int atpos = 0; + t = (tagStruct *) DBgetTags(xx,seq); + del_tag = NULL; + + while (t != NULL) { + if ( !(t->flags & TAG_DELETED) && + t->tagrec.position == npos && + strncmp(t->tagrec.type.c,TAG_TYPE_DELETE,3)==0) { + if (! atpos && t->tagrec.type.c[3] == base) del_tag = t; + atpos++; + } + if ( !(t->flags & TAG_DELETED) && + t->tagrec.position >= npos ) { + t->tagrec.position++; + t->flags |= TAG_POSITION_CHANGED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } + else if ( !(t->flags & TAG_DELETED) && + t->tagrec.position + t->tagrec.length > npos ) { + t->tagrec.length++; + t->flags |= TAG_LENGTH_CHANGED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } + t = t->next; + } + + if (del_tag == NULL) + tagEditInsert(xx, seq, npos,base); + else + _delete_tag(xx, seq, del_tag); +} + +void tagDeleteBase(EdStruct *xx,int seq,int pos, char *deletedBase) +/* +** A character has been deleted from position `pos' in sequence `seq' +** Adjust tag positions and lengths accordingly +*/ +{ + int npos = normalisePos(xx,seq,pos,0); + char base = normaliseBase(xx,seq,*deletedBase); + tagStruct *t; + + tagEditDelete(xx, seq, npos, base); + + t = (tagStruct *) DBgetTags(xx,seq); + while (t != NULL) { + if ( !(t->flags & TAG_DELETED) && + t->tagrec.position > npos) { + t->tagrec.position--; + t->flags |= TAG_POSITION_CHANGED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } + else if ( !(t->flags & TAG_DELETED ) && + t->tagrec.position + t->tagrec.length > npos) { + t->tagrec.length--; + t->flags |= TAG_LENGTH_CHANGED; + DBsetFlags(xx,seq,DBgetFlags(xx,seq)|DB_FLAG_TAG_MODIFIED); + } + t = t->next; + } + +} + +void tagReplaceBase(EdStruct *xx,int seq,int pos, char *deletedBase, char *insertedBase) +/* +** A character has been replaced in position `pos' in sequence `seq' +** Adjest tags accordingly +*/ +{ + int tpos = normalisePos(xx,seq,pos,1); + char dbase = normaliseBase(xx,seq,*deletedBase); + char ibase = normaliseBase(xx,seq,*insertedBase); + tagEditDelete(xx, seq, tpos, dbase); + tagEditInsert(xx, seq, tpos, ibase); +} + + +tagStruct *findTag(EdStruct *xx,int seq,int pos) +/* +** Find the tag (if any) at position `pos' in sequence `seq' +*/ +{ + int npos = normalisePos(xx,seq,pos,1/*character*/); + + tagStruct *t; + t = (tagStruct *) DBgetTags(xx,seq); + while (t != NULL) { + if (!(t->flags & TAG_DELETED) && + t->tagrec.position <= npos && + t->tagrec.position + t->tagrec.length > npos && + t->tagrec.type.c[0] != '*') + return t; + t = t->next; + } + return NULL; +} + +tagStruct *findAllTags(EdStruct *xx,int seq,int pos) +/* +** Find the tag (if any) at position `pos' in sequence `seq' +*/ +{ + static tagStruct *t; + static int npos; + if (xx==NULL) { + if (t != NULL) t = t->next; + } else { + npos = normalisePos(xx,seq,pos,1/*character*/); + t = (tagStruct *) DBgetTags(xx,seq); + } + + while (t != NULL) { + if (!(t->flags & TAG_DELETED) && + t->tagrec.position <= npos && + t->tagrec.position + t->tagrec.length > npos) + return t; + t = t->next; + } + return NULL; +} + +void writeTagList(EdStruct *xx, int seq) +{ + tagRecord anon_tagrec; + tagStruct *first_tag,*this_tag; + tag_id last_tagrec_id; + int flag; + + first_tag = (tagStruct *) DBgetTags(xx,seq); + + /* + ** *INS - flag as deleted all *INS flags with length 0 + */ + this_tag = first_tag; + while (this_tag != NULL) { + if ( !(this_tag->flags & TAG_DELETED) && + this_tag->tagrec.length == 0 && + strncmp(this_tag->tagrec.type.c,TAG_TYPE_INSERT,3)==0) + this_tag->flags |= TAG_DELETED; + this_tag = this_tag->next; + } + + /* + ** Assumption: first tag in list is always a header and doesn't change + */ + last_tagrec_id = DBgetNumber(xx,seq); + this_tag = first_tag->next; + + /* + ** Assumption: cutoff information is stored in header's comment + */ + if (first_tag->flags & TAG_COMMENT_CHANGED) { + if (first_tag->tagrec.comment) + (void) replace_comment(first_tag->tagrec.comment,first_tag->newcomment); + else { + first_tag->tagrec.comment = put_comment(first_tag->newcomment); + (void) write_tag(last_tagrec_id,first_tag->tagrec); + } + } + + while (this_tag != NULL) { + flag = this_tag->flags; + + if (flag == TAG_UNCHANGED || flag == TAG_COMMENT_IN_MEMORY) { + /* + ** get next tag in list + */ + last_tagrec_id = this_tag->original_tag_id; + this_tag = this_tag->next; + } + + else if (flag & TAG_INSERTED && !(flag & TAG_DELETED) ) { + tag_id this_tagrec_id; + /* + ** create a new tag + */ + this_tagrec_id = get_free_tag(); + /* + ** update previous tag record + */ + (void) read_tag(last_tagrec_id,&anon_tagrec); + this_tag->tagrec.next = anon_tagrec.next; + anon_tagrec.next = this_tagrec_id; + (void) write_tag(last_tagrec_id,anon_tagrec); + /* + ** write new tag record + */ + if (this_tag->newcomment!=NULL && this_tag->newcommentlen!=0) + this_tag->tagrec.comment = put_comment(this_tag->newcomment); + else + this_tag->tagrec.comment = 0; + (void) write_tag(this_tagrec_id,this_tag->tagrec); + /* + ** get next tag in list + */ + last_tagrec_id = this_tagrec_id; + this_tag = this_tag->next; + } + + else if (!(flag & TAG_INSERTED) && flag & TAG_DELETED) { + tag_id this_tagrec_id; + this_tagrec_id = this_tag->original_tag_id; + /* + ** delink record from file + */ + (void) read_tag(last_tagrec_id,&anon_tagrec); + anon_tagrec.next = this_tag->tagrec.next; + (void) write_tag(last_tagrec_id,anon_tagrec); + /* + ** destroy deleted tag + */ + (void) delete_tag_rec(this_tagrec_id); + /* + ** get next tag in list + */ + this_tag = this_tag->next; + } + + else if (!(flag & TAG_DELETED) ) + { + tag_id this_tagrec_id; + this_tagrec_id = this_tag->original_tag_id; + /* + ** Deal with comment first + */ + if (flag & TAG_COMMENT_CHANGED) { + if (this_tag->tagrec.comment) + (void) replace_comment(this_tag->tagrec.comment,this_tag->newcomment); + else + this_tag->tagrec.comment = put_comment(this_tag->newcomment); + } + (void) write_tag(this_tagrec_id,this_tag->tagrec); + /* + ** get next tag in list + */ + last_tagrec_id = this_tagrec_id; + this_tag = this_tag->next; + } + else { + /* + ** get next tag in list + */ + this_tag = this_tag->next; + } + } +} + + + +int origpos(EdStruct *xx, int seq, int pos) +/* +** Determine position in original sequence corresponding to pos +** taking into account all insertions and deletions +*/ +{ + int npos = pos; + + tagStruct *t; + t = (tagStruct *) DBgetTags(xx,seq); + while (t != NULL && + (t->tagrec.position <= pos || t->flags & TAG_DELETED)) { + if ( !(t->flags & TAG_DELETED ) && + t->tagrec.type.c[0] == '*') { + if (strncmp(t->tagrec.type.c, TAG_TYPE_INSERT,3)==0) + /*insertion*/ + npos--; + else if (strncmp(t->tagrec.type.c, TAG_TYPE_DELETE,3)==0) + /*deletion*/ + npos++; + } + t = t->next; + } + + return npos; +} + + + +tagStruct *findTagByType(EdStruct *xx, int seq, char *type) +/* +** Find the first tag by type +*/ +{ + tagStruct *t; + int looking,found; + + /* find tag */ + t = (tagStruct *) DBgetTags(xx,seq); + looking = 1; + found = 0; + while (looking && !found) { + found = ( strncmp( t->tagrec.type.c, type, 4 ) == 0 ); + if (! found) { + t = t->next; + looking = (t==NULL)?0:( !t->tagrec.position ); + } + } + if (found) + return t; + else + return NULL; +} + + +void getLeftCutOff(EdStruct *xx,int seq, int width, char *str) +{ + if (xx->reveal_cutoffs && width >0 ) { + tagStruct *t; + int c; + c = (DBgetComp(xx,seq)==COMPLEMENTED); + + /* find tag */ + t = findTagByType(xx,seq,(c?TAG_TYPE_RCUT:TAG_TYPE_LCUT)); + + if (t != NULL) { + /* + ** Find current comment + */ + force_comment(t); + + if (t->newcomment != NULL) { + int l = t->newcommentlen; + for (;l=0;width--) { + int_f i=1; + *str = t->newcomment[width]; + sqcom_(str,&i,(int_fl)1); + str++; + } + else + strncpy(str,&t->newcomment[l-width],width); + return; + } + } + } + + for(;width>0;width--)*str++=' '; +} + +void getLCut(EdStruct *xx,int seq, int pos, int width, char *str) +/* +** +*/ +{ + if (xx->reveal_cutoffs && width >0 ) { + tagStruct *t; + int c; + c = (DBgetComp(xx,seq)==COMPLEMENTED); + + /* find tag */ + t = findTagByType(xx,seq,(c?TAG_TYPE_RCUT:TAG_TYPE_LCUT)); + + if (t != NULL) { + /* + ** Find current comment + */ + force_comment(t); + + if (t->newcomment != NULL) { + int l = t->newcommentlen; + for (;l=0;width--,pos--) { + int_f i=1; + *str = t->newcomment[pos]; + sqcom_(str,&i,(int_fl)1); + str++; + } + else + strncpy(str,&t->newcomment[l-pos],width); + return; + } + } + } + + for(;width>0;width--)*str++=' '; +} + + +void getRightCutOff(EdStruct *xx,int seq, int width, char *str) +{ + if (xx->reveal_cutoffs && width >0 ) { + tagStruct *t; + int c; + c = (DBgetComp(xx,seq)==COMPLEMENTED); + + /* find tag */ + t = findTagByType(xx,seq,(c?TAG_TYPE_LCUT:TAG_TYPE_RCUT)); + + if (t != NULL) { + /* + ** Find current comment + */ + force_comment(t); + + if (t->newcomment != NULL) { + int l = t->newcommentlen; + for (;lnewcomment[l-1];width>0;width--,p--) { + int_f i=1; + *str = *p; + sqcom_(str,&i,(int_fl)1); + str++; + } + } else + strncpy(str,t->newcomment,width); + return; + } + } + } + + for(;width>0;width--)*str++=' '; +} + + +void getRCut(EdStruct *xx,int seq, int pos, int width, char *str) +{ + if (xx->reveal_cutoffs && width >0 ) { + tagStruct *t; + int c; + c = (DBgetComp(xx,seq)==COMPLEMENTED); + + /* find tag */ + t = findTagByType(xx,seq,(c?TAG_TYPE_LCUT:TAG_TYPE_RCUT)); + + if (t != NULL) { + /* + ** Find current comment + */ + force_comment(t); + + if (t->newcomment != NULL) { + int l = t->newcommentlen; + for (;lnewcomment[l-pos-1];width>0;width--,p--) { + int_f i=1; + *str = *p; + sqcom_(str,&i,(int_fl)1); + str++; + } + } else + strncpy(str,&t->newcomment[pos],width); + return; + } + } + } + + for(;width>0;width--)*str++=' '; +} + + +int lenRCut(EdStruct *xx, int seq) +{ + tagStruct *t; + int c; + c = (DBgetComp(xx,seq)==COMPLEMENTED); + + /* find tag */ + t = findTagByType(xx,seq,(c?TAG_TYPE_LCUT:TAG_TYPE_RCUT)); + + if (t != NULL) { + /* + ** Find current comment + */ + force_comment(t); + + if (t->newcomment != NULL) + return t->newcommentlen; + else + return 0; + } else + return 0; + +} + + +int lenLCut(EdStruct *xx, int seq) +{ + tagStruct *t; + int c; + c = (DBgetComp(xx,seq)==COMPLEMENTED); + + /* find tag */ + t = findTagByType(xx,seq,(c?TAG_TYPE_RCUT:TAG_TYPE_LCUT)); + + if (t != NULL) { + /* + ** Find current comment + */ + force_comment(t); + + if (t->newcomment != NULL) + return t->newcommentlen; + else + return 0; + } else + return 0; + +} + +void dump_tags(EdStruct *xx, int seq) +{ + tagStruct *t = (tagStruct *) DBgetTags(xx,seq); + + fprintf(stderr,"Tags for %s\n",DBgetName(xx,seq)); + while (t != NULL) { + + fprintf(stderr," %5d %3d %4s %5d %c%c%c%c%c%c%c\n", + t->tagrec.position, + t->tagrec.length, + t->tagrec.type.c, + t->tagrec.comment, + (t->flags & TAG_POSITION_CHANGED) ?'P':'-', + (t->flags & TAG_LENGTH_CHANGED) ?'L':'-', + (t->flags & TAG_TYPE_CHANGED) ?'T':'-', + (t->flags & TAG_COMMENT_CHANGED) ?'C':'-', + (t->flags & TAG_INSERTED) ?'I':'-', + (t->flags & TAG_DELETED) ?'D':'-', + (t->flags & TAG_COMMENT_IN_MEMORY)?'M':'-' + ); + + t = t->next; + } +} diff --git a/src/staden/tagU2.c b/src/staden/tagU2.c new file mode 100644 index 0000000..763fdfc --- /dev/null +++ b/src/staden/tagU2.c @@ -0,0 +1,706 @@ +#include "contigEditor.h" +#include +#include +#include "tagUtils.h" +#include "fort.h" + +#define COMMENT_HEAD_ID (1) +#define TAG_HEAD_ID (devils_.idbsiz) + +int read_tag(tag_id n, tagRecord *t) +{ + readtg_(&devils_.idevt,&n,&t->position,&t->length,&t->comment,&t->type.i,&t->next); + return 0; +} +int write_tag(tag_id n, tagRecord t) +{ + writtg_(&devils_.idevt,&n,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + return 0; +} +int read_comment(comment_id n, int *cnt, commentStruct *c) +{ + readcc_(&devils_.idevc,&n,cnt,&c->next,&c->comment[0],40); + return 0; +} +int write_comment(comment_id n, int cnt, commentStruct c) +{ + writcc_(&devils_.idevc,&n,&cnt,&c.next,&c.comment[0],40); + return 0; +} +/* comment interface */ +tag_id get_free_tag() +{ + tagRecord head; + tagRecord freerec; + tag_id free_id; + (void) read_tag(TAG_HEAD_ID,&head); + if (head.next != 0) { + /* + ** if a free slot somewhere, use it + */ + free_id = head.next; + (void) read_tag(free_id,&freerec); + head.next = freerec.next; + (void) write_tag(TAG_HEAD_ID,head); + } else { + /* + ** extend comment list file + */ + free_id = ++head.position; + (void) write_tag(TAG_HEAD_ID,head); + (void) write_tag(free_id,freerec); + } + + return free_id; +} + +void delete_comment (comment_id cp) +{ + int dummy; + commentStruct head; + commentStruct freerec; + comment_id free_id; + if (!cp) return; + free_id = cp; + (void) read_comment(free_id,&dummy,&freerec); + while (freerec.next) { + free_id = freerec.next; + (void) read_comment(free_id,&dummy,&freerec); + } + (void) read_comment(COMMENT_HEAD_ID,&dummy,&head); + freerec.next = head.next; + (void) write_comment(free_id,dummy,freerec); + head.next = cp; + (void) write_comment(COMMENT_HEAD_ID,dummy,head); +} + + + + + +void blank_tag_rec(tag_id t) +/* +** Blank out fields in tag record t +*/ +{ + + tagRecord r; + + (void) read_tag(t, &r); + + r.position = 0; + r.length = 0; + r.type.i = 0x20202020; + r.comment = 0; + r.next = 0; + + (void) write_tag(t, r); + +} + + + + +void delete_tag_rec(tag_id t) +/* +** remove t from file, discarding comment if necessary +*/ +{ + tagRecord head; + tagRecord freerec; + + (void) read_tag(t,&freerec); + + if (freerec.comment) + delete_comment(freerec.comment); + + (void) read_tag(TAG_HEAD_ID,&head); + freerec.next = head.next; + (void) write_tag(t,freerec); + head.next = t; + (void) write_tag(TAG_HEAD_ID,head); + +} + +static comment_id get_free_comment() +{ + commentStruct head; + commentStruct freerec; + comment_id free_id; + int count; + (void) read_comment(COMMENT_HEAD_ID,&count,&head); + if (head.next != 0) { + /* + ** if a free slot somewhere, use it + */ + free_id = head.next; + (void) read_comment(free_id,&count,&freerec); + head.next = freerec.next; + (void) write_comment(COMMENT_HEAD_ID,count,head); + } else { + /* + ** extend comment list file + */ + free_id = ++count; + (void) write_comment(COMMENT_HEAD_ID,free_id,head); + (void) write_comment(free_id,0,freerec); + } + + return free_id; +} + +comment_id put_comment(char *c) +{ + commentStruct com; + comment_id cur,next,this_comment; + size_t clen = strlen(c); + size_t piece; + + /* write out first block of COMMENT_LENGTH */ + this_comment=cur=get_free_comment(); + if (clen>COMMENT_LENGTH) + piece = COMMENT_LENGTH; + else + piece = clen; + + {int i; for(i=0;i 0) { + next = get_free_comment(); + com.next = next; + write_comment(cur,0,com); + cur = next; + if (clen=0;i--) { + new_id = get_free_tag(); + new_t.position = pos+i; + new_t.next = next_id; + writtg_(&devils_.idevt,&new_id,&new_t.position,&new_t.length,&new_t.comment,&new_t.type.i,&new_t.next); + next_id = new_id; + } + t.next=next_id; + writtg_(&devils_.idevt,&t_id,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + +} + +static void myFstr2Cstr(char *Fstr, int_fl Flen, + char *Cstr, int_fl Clen) +/* + Copy the significant characters of a blank padded Fortran string + to a '\0' terminated C string, ignoring excess characters. + + This function works if the strings are distinct or coincident, but + not if they overlap in any other way. +*/ +{ int_fl FsigLen, i; + + /* Find the significant length of Fstr */ + FsigLen=Flen; + while ((FsigLen > 0) && (Fstr[FsigLen-1] == ' ')) + { FsigLen--; + } + + /* Copy up to (Clen-1) significant characters */ + i=0; + while ((i < FsigLen) && (i < (Clen-1))) + { Cstr[i] = Fstr[i]; + i++; + } + + Cstr[i] = '\0'; +} + +/* C version of ENTRD */ +void entrd_(int_f *IDEVG,int_f *IDEVT,int_f *IDEVC,char *NAMARC,int_f *NGEL, + int_f *IOK, int_fl l_NAMARC) +{ +/* + char NAMFIL[FILE_NAME_LENGTH], MTYPE[4]; + char namfil[FILE_NAME_LENGTH+1], mtype[5]; +*/ + int LENR,LCUT,LENW; + int RCUT; + +#define fn_len 100 + char fn[fn_len]; +#define l_line 100 + char line[l_line]; + char *result; + char *cutbuff,*cb,*l; + + FILE *fp; + + /* blank tag rec */ + blank_tag_rec((tag_id) *NGEL); + + myFstr2Cstr(NAMARC,l_NAMARC,fn,(int_fl)fn_len); + + *IOK = 0; + /* don't need this anymore + saveState.idevt = &devils_.idevt; + saveState.idevc = &devils_.idevc; + saveState.idbsiz = &devils_.idbsiz; + */ + /* Enters raw data info into database */ + if ( (fp = fopen(fn,"r")) != NULL ) { + result = fgets(line,l_line,fp); + if (result != NULL && line[0] == ';') { + /* process header */ + sscanf(line,";%6d%6d%6d%*s\n",&LENR,&LCUT,&LENW); + RCUT = LENR - LCUT - LENW; + add_RD_comment(*NGEL, &line[1]); + result = fgets(line,l_line,fp); + } + if (result != NULL && strlen(line)>1 && strncmp(line,";<",2)==0) { + /* process left cut off */ + /* starting with this line we need to read LCUT ditties */ + cutbuff = (char *) TAG_MALLOC(LCUT+1); + cb = cutbuff; + while (result!=NULL && strlen(line)>1 && strncmp(line,";<",2)==0){ + for(l=&line[2];*l;l++)if(strchr(" \n\t",*l)==NULL)*cb++=*l; + result = fgets(line,l_line,fp); + } + *cb='\0'; + insert_CUT_tags(*NGEL, 0, 0, TAG_TYPE_LCUT, cutbuff); + TAG_FREE(cutbuff); + } + + if (result != NULL && strlen(line)>1 && strncmp(line,";>",2)==0) { + /* process right cut off */ + /* starting with this line we need to read RCUT ditties */ + cutbuff = (char *) TAG_MALLOC(RCUT+1); + cb = cutbuff; + while (result != NULL && strlen(line)>1 && strncmp(line,";>",2)==0) { + for(l=&line[2];*l;l++)if(strchr(" \n\t",*l)==NULL)*cb++=*l; + result = fgets(line,l_line,fp); + } + *cb='\0'; + insert_CUT_tags(*NGEL, 0, 0, TAG_TYPE_RCUT, cutbuff); + TAG_FREE(cutbuff); + } + + fclose(fp); + } +} + +int_f freecc_ (int_f *IDEVC) +{ + return get_free_comment(); +} + +void padtag_(int_f *llino_, int_f *k_, int_f *nc_, int_f *lngthg_) +/* +** Play around with tags when auto assembling +*/ +{ + int seq; + int pos; + int length; + int seq_length; + tagRecord t; + tag_id last,next; + int last_pos; + if (devils_.idevt>0) { + /* fudge */ + /* don't need this anymore + saveState.idevt = &devils_.idevt; + saveState.idbsiz = &devils_.idbsiz; + */ + seq = *llino_; + length = *nc_; + if (*lngthg_ < 0) { + seq_length = - *lngthg_; + pos = seq_length - *k_ - *nc_ + 2; + } else { + seq_length = *lngthg_; + pos = *k_; + } + + next = seq; + last = 0; + last_pos = 0; + while (next) { + readtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + /* + ** Move tags accordingly + */ + if (t.position >= pos) + { + t.position+=length; + writtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + } + else if (t.position + t.length > pos) + { + t.length+=length; + writtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + } + + /* + ** Insert *INS tags + */ + if (last_pos < pos && t.position >= pos) + /* + ** Insert after last `length' tags + */ + insert_INS_tags(last,pos,length); + else if (t.position < pos && t.next==0) + /* + ** Insert after next `length' tags + */ + insert_INS_tags(next,pos,length); + + last = next; + last_pos = t.position; + next = t.next; + } + + } +} + +void taggel_(int_f *ngels_, int_f *lngthg_, char *gel_, int_fl gel_length) +{ + int_f length = abs(*lngthg_); + int_f one=1; + int_f i,j; + + if (*lngthg_ > 0) { + for (i=0,j=1; i=0; i--,j--) + if (gel_[i]=='*') + padtag_(ngels_,&j,&one,lngthg_); + } + +} + +void movtag_ (int_f *from, int_f *to ) +/* +** Move tag information of gel ``from'' to gel ``to'', +** and perform garbage collection on old gel ``to'' +*/ +{ + + tagRecord freerec; + tag_id this,next; + + /* don't do anything if tag files aren't open */ + if ( devils_.idevc < 0 || devils_.idevt < 0 ) return; + + /* Throw away ``to'' tag records */ + (void) read_tag((tag_id) *to,&freerec); + + if (freerec.comment) + delete_comment(freerec.comment); + + next = freerec.next; + while ( next ) { + this = next; + (void) read_tag( this , &freerec ); + next = freerec.next; + delete_tag_rec (this); + } + + /* copy ``from'' record to ``to'' record */ + (void) read_tag((tag_id) *from,&freerec); + (void) write_tag((tag_id) *to,freerec); + + /* blank out old tag rec */ + blank_tag_rec(*from); + +} + +char *get_comment(comment_id cp) +{ + int dummy; + commentStruct c; + int count; + comment_id nc; + char *com,*comptr; + + if (!cp) return NULL; + /* determine how long string is */ + count = 1; + nc=cp; + read_comment(nc, &dummy, &c); + while (c.next != 0) { + nc = c.next; + count++; + read_comment(nc, &dummy, &c); + } + + com = comptr = (char *)TAG_MALLOC(count * COMMENT_LENGTH+1); + nc=cp; + read_comment(nc, &dummy, &c); + strncpy(com,c.comment,COMMENT_LENGTH); com+=COMMENT_LENGTH; + while (c.next != 0) { + nc = c.next; + count++; + read_comment(nc, &dummy, &c); + strncpy(com,c.comment,COMMENT_LENGTH); com+=COMMENT_LENGTH; + } + *com = '\0'; + + return comptr; + +} + +void getext_(int_f *gel, char *cutoff, int_f *lcutoff, int_f *ok, + int_fl l_cutoff) +/* +** Get right cutoff for lowly Fortran Users +*/ +{ + + tagRecord rec; + tag_id next; + + (void) read_tag((tag_id) *gel,&rec); + + next = rec.next; + + while ( next && ! rec.position ) { + (void) read_tag( next , &rec ); + next = rec.next; + + if (strncmp(rec.type.c,TAG_TYPE_RCUT,4) == 0) { + if (rec.comment) { + char *c; + int i; + /* use this */ + c = get_comment(rec.comment); + /* copy to fortran array */ + for (i=0; i<*lcutoff && c[i]; i++) cutoff[i] = c[i]; + /* set length of returned string */ + *lcutoff = i; + for (; i<*lcutoff; i++) cutoff[i] = ' '; + + /* rec temporary string space */ + TAG_FREE(c); + *ok = 0; + return; + } else { + *ok = 1; + return; + } + + } + } + + *ok = 1; +} + + + + + + + + + + + +/*************************************************************/ +/** Routines for Rodger's pad shuffling */ +/*************************************************************/ + +static void insert_edit_tag(tag_id t_id, int pos, char base, char *type) +{ + tagRecord t,new_t; + tag_id new_id; + + /* read previous tag */ + readtg_(&devils_.idevt,&t_id,&t.position,&t.length,&t.comment, + &t.type.i,&t.next); + + /* set up new record */ + strncpy(new_t.type.c,type,3); + new_t.type.c[3]=base; + new_t.length = 1; + new_t.comment = 0; + new_t.position = pos; + new_t.next = t.next; + + /* write new record */ + new_id = get_free_tag(); + writtg_(&devils_.idevt,&new_id,&new_t.position,&new_t.length,&new_t.comment,&new_t.type.i,&new_t.next); + + /* update previous tag */ + t.next=new_id; + writtg_(&devils_.idevt,&t_id,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + +} + + + +void insedt_(int_f *seq_, int_f *pos_, char *base_, int_fl base_l) +/* +** Insert a tag for a base at position pos in gel +*/ +{ + int seq; + int pos; + char base; + + tagRecord t; + tag_id last,next; + + if (devils_.idevt>0) { + + seq = (int) *seq_; + pos = (int) *pos_; + base = base_[0]; + + + /* skip over header */ + last = next = seq; + readtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + next = t.next; + if (next) readtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + + /* adjust lengths of all tags staring before pos */ + while (next && t.position < pos) { + if (t.position + t.length - 1 >= pos && + t.type.c[0] != '*') { + t.length++; + writtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + } + last = next; + next = t.next; + if (next) readtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + + } + + /* insert a tag after last and before next */ + insert_edit_tag(last,pos,base,TAG_TYPE_INSERT); + + /* shift everything else right */ + while (next) { + t.position++; + writtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + next = t.next; + if (next) readtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + } + + } +} + + + + + +void deledt_(int_f *seq_, int_f *pos_, char *base_, int_fl base_l) +/* +** Delete a base at position pos in gel +*/ +{ + int_f seq; + int_f pos; + char base; + + tagRecord t; + tag_id last,next; + + if (devils_.idevt>0) { + + seq = *seq_; + pos = *pos_; + base = base_[0]; + + + /* skip over header */ + last = next = (tag_id)seq; + readtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + next = t.next; + if (next) readtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + + /* adjust lengths of all tags staring before pos */ + while (next && t.position <= pos) { + if (t.position + t.length - 1 >= pos && + t.type.c[0] != '*') { + t.length--; + writtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + } + last = next; + next = t.next; + if (next) readtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + + } + + /* insert a tag after last and before next */ + insert_edit_tag(last,pos,base,TAG_TYPE_DELETE); + + /* shift everything else right */ + while (next) { + t.position--; + writtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + next = t.next; + if (next) readtg_(&devils_.idevt,&next,&t.position,&t.length,&t.comment,&t.type.i,&t.next); + } + + } +} diff --git a/src/staden/tagUtils.h b/src/staden/tagUtils.h new file mode 100644 index 0000000..f26153f --- /dev/null +++ b/src/staden/tagUtils.h @@ -0,0 +1,146 @@ +#ifndef _tagUtils_h +#define _tagUtils_h + +#include "fortran.h" +#include "fort.h" +/* #include "Sheet.h" */ +/* +** The following describe two database files: +** The tag list file +** The comment list file +** +** the tag list file consists of at least IDBSIZ records. +** +** 1 : header tag for sequence 1 +** 2 : header tag for sequence 2 +** ... +** IDBSIZ-1: header tag for sequence IDBSIZ-1: +** IDBSIZ : descriptor record defining MAX_TAG +** IDBSIZ+1: supplimentary tags +** ... +** MAX_TAG : supplimentary tags +** +** the comment list file consists of at least 1 record. +** +** 1 : descriptor record defining MAX_COM +** 2 : supplimentary comment +** ... +** MAX_COM : supplimentary comment +** +*/ + +#define COMMENT_LENGTH 40 +/* COMMENT_LENGTH is hard coded in dbsysnew.f */ +/* grep for COMMENT_LENGTH */ +#define FILE_NAME_LENGTH 18 +/* FILE_NAME_LENGTH is hard coded in dbsysnew.f */ +/* grep for FILE_NAME_LENGTH */ + +typedef int_f tag_id; +typedef int_f comment_id; + +/* +** element in tag list +*/ +typedef union { + char c[4]; + int_f i; + } tag_types; + +typedef struct _tagRecord{ + int_f position; /* position in sequence */ + int_f length; /* length of tag */ + tag_types type; + comment_id comment; /* index to comment */ + tag_id next; /* link to next in structure */ +} tagRecord; + +/* +** comment chain +*/ +typedef struct _commentStruct{ + char comment[COMMENT_LENGTH]; + comment_id next; +} commentStruct; + +typedef struct _tagstruct{ + /* + ** Data from the original file + */ + tagRecord tagrec; + /* + ** Data for database management + */ + tag_id original_tag_id; + char *newcomment; + int newcommentlen; + long flags; + int time; + struct _tagstruct *next; +} tagStruct,*tagptr; + +#define TAG_UNCHANGED (0) +#define TAG_POSITION_CHANGED (1L<<1) +#define TAG_LENGTH_CHANGED (1L<<2) +#define TAG_TYPE_CHANGED (1L<<3) +#define TAG_COMMENT_CHANGED (1L<<4) +#define TAG_INSERTED (1L<<5) +#define TAG_DELETED (1L<<6) +#define TAG_COMMENT_IN_MEMORY (1L<<7) + +#define TAG_MALLOC(s) (char *)malloc(s) +#define TAG_FREE(c) free(c) + +#define TAG_TYPE_INSERT "*INS" +#define TAG_TYPE_DELETE "*DEL" +#define TAG_TYPE_LCUT "*LC*" +#define TAG_TYPE_RCUT "*RC*" +#define TAG_TYPE_RAWDATA "*RAW" + +#include "edUtils.h" + +/* define external routines */ +extern void force_comment(tagStruct *t); + +/* comment interface */ +extern tagStruct *readTagList(int seq); +extern void writeTagList(EdStruct *xx, int seq); +extern void destroyTagList(tagStruct *s); + +extern void createTag(EdStruct *xx); +extern void editTag(EdStruct *xx); +extern void deleteTag(EdStruct *xx); +/* extern void getTagSplodge(EdStruct *xx, int seq, int pos, int width, XawSheetInk *ink); */ + +extern void insertTag(EdStruct *xx, int seq, tagStruct *t); +extern tagStruct *newTag(); +/* this gives me lots of problems...why? +extern char normaliseBase(EdStruct *xx,int seq,char deletedBase); +*/ + +extern void tagInsertBase(EdStruct *xx,int seq,int pos,char *base); +extern void tagDeleteBase(EdStruct *xx,int seq,int pos,char *base); +extern void tagReplaceBase(EdStruct *xx,int seq,int pos,char *dbse,char *ibse); +extern tagStruct *findTag(EdStruct *xx,int seq,int pos); +extern int origpos(EdStruct *xx, int seq, int pos); +extern void getLeftCutOff(EdStruct *xx,int seq, int width, char *str); +extern void getRightCutOff(EdStruct *xx,int seq, int width, char *str); +extern char *get_comment(comment_id cp); +extern int lenLCut(EdStruct *xx, int seq); +extern int lenRCut(EdStruct *xx, int seq); +extern void getLCut(EdStruct *xx,int seq, int pos, int width, char *str); +extern void getRCut(EdStruct *xx,int seq, int pos, int width, char *str); +extern tagStruct *findTagByType(EdStruct *xx, int seq, char *type); +extern char normaliseBase(EdStruct *xx,int seq,char deletedBase); +extern void dump_tags(EdStruct *xx, int seq); +extern void setButtonName(Widget w, char *c); +extern void createTagTypeMenu(Widget parent, void (*call_back)() ); +extern void delete_comment (comment_id cp); +extern comment_id put_comment(char *c); +extern int read_tag(tag_id n, tagRecord *t); +extern int invokeTagEditor(char *type_id, char *newType, char *tagComment, char **newComment); +extern int write_tag(tag_id n, tagRecord t); +extern tag_id get_free_tag(); +extern void delete_tag_rec(tag_id t); + +#endif /* _tagUtils_h */ diff --git a/src/staden/tagdb.h b/src/staden/tagdb.h new file mode 100644 index 0000000..8ecc875 --- /dev/null +++ b/src/staden/tagdb.h @@ -0,0 +1,35 @@ +#ifndef _tagdb_h +#define _tagdb_h + +#include + +#define MAX_TAG_TYPES 256 + +#define TAG_UNKNOWN 0 +#define TAG_SEARCH_ID 1 +#define TAG_FG_COLOUR 2 +#define TAG_BG_COLOUR 3 +#define TAG_DEFAULT_TEXT 4 + +typedef struct { + /* + ** values taken from TAGDB file + */ + char *type; + char *search_id; + char *fg_colour; + char *bg_colour; + char *default_text; + /* + ** values derived from above + */ + Pixel fg_pixel; + Pixel bg_pixel; + char id[4]; + } tag_db_struct; + +extern tag_db_struct tag_db[MAX_TAG_TYPES]; +extern int tag_db_count; +extern void parse(char *file); + +#endif /* _tagdb_h */ diff --git a/src/staden/tagdbparse.c b/src/staden/tagdbparse.c new file mode 100644 index 0000000..31e97fe --- /dev/null +++ b/src/staden/tagdbparse.c @@ -0,0 +1,327 @@ +#include +#include +#include +#include "tagdb.h" + +/* TOKENS */ +#define NULLTOKEN 0 +#define END 1 +#define ID 2 +#define SEPARATOR 3 +#define NL 4 +#define EQ 5 + +static char *infile; /* file being parsed */ + +static char word[128]; +static int lineno; + +/* ---------------- private routines ----------------- */ +static void spring_clean_text (s,t) + /* parse out normal escape characters bar hex and octal */ + char *s; + char *t; +{ + while (*s != '\0') { + if (*s == '\\') + switch (*++s) { + case 'a': *t++ = '\a'; s++; break; + case 'b': *t++ = '\b'; s++; break; + case 'f': *t++ = '\f'; s++; break; + case 'n': *t++ = '\n'; s++; break; + case 'r': *t++ = '\r'; s++; break; + case 't': *t++ = '\t'; s++; break; + case 'v': *t++ = '\v'; s++; break; + case '\n': + while (isspace(*++s)); + break; + /* + case 'x' : + if (isxdigit(s[1]) && isxdigit(s[2])) { + #define ASCII_TO_HEX(A) (isdigit(A)?(A-'0'):(tolower(A)-'a'+10)) + *t++ = ASCII_TO_HEX(s[1])*16 + ASCII_TO_HEX(s[2]); + s++;s++;s++; + } + break; + */ + case '\\': + case '\?': + case '\'': + case '\"': + case '\0': break; + } else *t++ = *s++; + } + *t = '\0'; + +} + + +static int next_word(FILE *fp) +/* + ** lexical analyser + ** Get's the next word from the input stream + */ +{ + int a; + int token; + char *s; + + token = NULLTOKEN; + s = word; + + while (token == NULLTOKEN) { + switch (a = getc(fp)) { + case EOF: + token = END; + break; + case '\n': + lineno++; + token = NL; + break; + case '=': + token = EQ; + break; + case ':': + token = SEPARATOR; + break; + case '#': + /* comment: skip to end of line */ + for(a=getc(fp); a!=EOF && a!='\n'; a=getc(fp)); + if (a=='\n') lineno++; + if (a==EOF) token = END; + break; + case '\\': + /* back quoted newlines are skipped */ + /* back quoted "anything else" is "anything else" */ + a = getc(fp); + if (a != EOF && a != '\n') ungetc(a,fp); + break; + case '"': + /* quoted string */ + for(a=getc(fp);a!=EOF && a!='"';a=getc(fp)) { + if (a=='\n') lineno++; + *s++ = a; + } + token = ID; + break; + default: + if (isalnum(a)) { + *s++ = a; + for(a=getc(fp);a!=EOF && isalnum(a);a=getc(fp)) *s++ = a; + if (a!=EOF) ungetc(a,fp); + token = ID; + } + break; + } + } + + *s = '\0'; + spring_clean_text(word,word); + return token; + +} + + +static void parse_error(char *s) +{ + fprintf( stderr, "%s on line %d of %s\n", s,lineno,infile); +} + +static int snatch(char *s) +{ + if (strcmp(s,"id")==0) return TAG_SEARCH_ID; + if (strcmp(s,"fg")==0) return TAG_FG_COLOUR; + if (strcmp(s,"bg")==0) return TAG_BG_COLOUR; + if (strcmp(s,"dt")==0) return TAG_DEFAULT_TEXT; + return TAG_UNKNOWN; +} + +static void initTagDBFields(char *type) +{ + tag_db[tag_db_count].type = (char *)malloc(strlen(type)+1); + strcpy(tag_db[tag_db_count].type,type); + tag_db[tag_db_count].search_id = NULL; + tag_db[tag_db_count].fg_colour = NULL; + tag_db[tag_db_count].bg_colour = NULL; + tag_db[tag_db_count].default_text = NULL; +} + +static void tidyUpTagDBFields() +{ + int len; + + if (tag_db[tag_db_count].search_id == NULL) { + tag_db[tag_db_count].search_id = tag_db[tag_db_count].type; + } + len = strlen(tag_db[tag_db_count].search_id); + if (len < 4) + strncpy(tag_db[tag_db_count].id," ",4); + else + len = 4; + strncpy(tag_db[tag_db_count].id,tag_db[tag_db_count].search_id,len); + + if (tag_db_count < (MAX_TAG_TYPES-1)) + tag_db_count++; +} + + + + + +static int parse_file(FILE *fp) + /* + ** Parse file and load info into tag_db[] + */ +{ + int at_end_of_file; + int at_end_of_entry; + + /* for safe keeping */ + int field; + char *WORD; + + at_end_of_file = 0; + + while (!at_end_of_file) { + switch (next_word(fp)) { + case END: + at_end_of_file = 1; + case NL: + break; + case ID: + /* the big time */ + initTagDBFields(word); + switch(next_word(fp)) { + case END: + at_end_of_file = 1; + case NL: + break; + case SEPARATOR: + /* arglist */ + at_end_of_entry = 0; + while (!at_end_of_entry) { + switch(next_word(fp)) { + case SEPARATOR: + break; + case ID: + field = snatch(word); + switch(next_word(fp)) { + case EQ: + switch(next_word(fp)) { + case ID: + WORD = (char *)malloc(strlen(word)+1); + strcpy(WORD,word); + switch (field) { + case TAG_SEARCH_ID : + tag_db[tag_db_count].search_id = WORD; + break; + case TAG_FG_COLOUR : + tag_db[tag_db_count].fg_colour = WORD; + break; + case TAG_BG_COLOUR : + tag_db[tag_db_count].bg_colour = WORD; + break; + case TAG_DEFAULT_TEXT : + tag_db[tag_db_count].default_text = WORD; + break; + default: + break; + } + switch(next_word(fp)) { + case END: + at_end_of_file = 1; + case NL: + at_end_of_entry = 1; + case SEPARATOR: + break; + default: + parse_error("Syntax error"); + return ( 1 ); + } + break; + default: + parse_error("Syntax error"); + return ( 1 ); + } + break; + case END: + at_end_of_file = 1; + case NL: + at_end_of_entry = 1; + case SEPARATOR: + break; + default: + parse_error("Syntax error"); + return ( 1 ); + } + break; + case END: + at_end_of_file = 1; + case NL: + at_end_of_entry = 1; + break; + default: + parse_error("Syntax error"); + return ( 1 ); + } + } + break; + default: + parse_error("Syntax error"); + return 1; + } + tidyUpTagDBFields(); + break; + default: + parse_error("Syntax error"); + return 1; + } + } + + return 0; +} + + + + + +/* ------------------- exported routines ------------------ */ + +tag_db_struct tag_db[MAX_TAG_TYPES]; +int tag_db_count; + +void parse(char *file) +{ + int parse_failed; + FILE *fp; + + char *default_type = "Comment"; + char *default_search_id = "DEFC"; + char *default_fg_colour = NULL; + char *default_bg_colour = "yellow"; + char *default_default_text = "Enter your comment here"; + + infile = file; + tag_db_count = 0; + + if(fp = fopen(file,"r")) { + lineno = 1; + parse_failed = parse_file(fp); + if (parse_failed) { + fprintf (stderr,"Error encountered while parsing tag database\nGiving up\n"); + tag_db_count = 0; + } + fclose(fp); + } else + fprintf (stderr,"Error parsing tag database\nFile `%s' does not exist\nGiving up\n",infile); + + if (! tag_db_count) { + tag_db[tag_db_count].type = default_type; + tag_db[tag_db_count].search_id = default_search_id; + tag_db[tag_db_count].fg_colour = default_fg_colour; + tag_db[tag_db_count].bg_colour = default_bg_colour; + tag_db[tag_db_count].default_text = default_default_text; + tag_db_count++; + } + +} diff --git a/src/staden/textOutput.c b/src/staden/textOutput.c new file mode 100644 index 0000000..7671edd --- /dev/null +++ b/src/staden/textOutput.c @@ -0,0 +1,191 @@ +/* + Title: textOutput + + File: textOutput.h + Purpose: Manage a text widget displaying stdout + Last update: Thur May 17 1990 +*/ + + +/* + This module creates a text widget, and arranges for output + from stdout to be displayed. The function UpdateTextOutput() + must be called periodically to keep the display up to date. + + It works by redirecting stdout to a temporary file. The contents + of this are retrieved everytime UpdateTextOutput() is called and + they are appended to the (string) text widget. The temporary + file is truncated each time, to keep it small, but the text + widget may grow arbitrarily large. + + To position the caret at the end of the file we use the value + MAXLONG. This is dodgy on two counts: + (1) It assumes XawTextPosition is a long. + (2) It assumes all routines interpret positions past the end of + the file as implying the end of the file. +*/ + + + + +/* ---- Includes ---- */ + +#include "textOutput.h" +#include "main.h" /* IMPORT: finalx */ +#include "mcspec.h" /* IMPORT: remove */ + +#include /* IMPORT: fopen, fclose, freopen, fprintf, fread, remove, + BUFSIZ, FILE, tmpnam, L_tmpnam */ +#include "values.h" /* IMPORT: MAXLONG */ + +#include +#include +#include + + +/* ---- Global variables ---- */ + +extern Dimension dialogue_width; +extern Dimension dialogue_height; + +/* ---- Static variables ---- */ +#ifndef L_tmpnam +#define L_tmpnam 25 /* (sizeof(P_tmpdir) + 15) */ +#endif + +static Widget textOutputWidget; +static char textLogFN[L_tmpnam]; +static FILE *textLogFP; + + + + +/* ---- Callback routines ---- */ + + +static void destroyCallback(Widget w, XtPointer client_data, XtPointer call_data) +{ (void) fclose(textLogFP); + (void) remove(textLogFN); +} + + + + +/* ---- Exported functions ---- */ + + +Widget CreateTextOutput(Widget parentWid) +/* + Create the text widget as a child of `parentWid'. + `stdout' may be redirected, but is not closed. + The display is initially empty. +*/ +{ static Arg arglist[] = + { {XtNeditType, (XtArgVal) XawtextEdit}, + {XtNscrollVertical, (XtArgVal) XawtextScrollAlways}, + {XtNwrap, (XtArgVal) XawtextWrapLine}, + }; + + /* + Create, or truncate, a temporary file for output + redirecting everything from stdout there. + Also open it for reading. + */ + if ( (freopen(tmpnam(textLogFN), "w", stdout) == NULL) + || ((textLogFP = fopen(textLogFN, "r")) == NULL) + ) + { fprintf(stderr,"\n*** Failed to open temporary file. Exiting.\n"); + finalx(1); + } + + + textOutputWidget = XtCreateManagedWidget("text", asciiTextWidgetClass, + parentWid, + arglist, XtNumber(arglist)); + + XawTextSetInsertionPoint(textOutputWidget, (XawTextPosition)MAXLONG); + XawTextDisplayCaret(textOutputWidget, False); + XtAddCallback(textOutputWidget, XtNdestroyCallback, destroyCallback, NULL); + + XtVaSetValues(textOutputWidget, XtNwidth, dialogue_width, + XtNheight, dialogue_height / 2, NULL); + + return(textOutputWidget); +} + + +void updout_() +{ + UpdateTextOutput(); +} + + +void UpdateTextOutput() +/* + Any text written to `stdout' since the last call of + UpdateTextOutput() is added to the display. +*/ +{ XawTextBlock text; + char buf[BUFSIZ]; + XawTextPosition pos; + + + /* Flush all output at the FORTRAN & C level*/ + flusho_(); + fflush(stdout); + + /* Get the end-of-text position, in a rather dodgy way */ + XawTextSetInsertionPoint(textOutputWidget, (XawTextPosition)MAXLONG); + pos = XawTextGetInsertionPoint(textOutputWidget); + + text.firstPos = 0; + text.ptr = buf; + text.format = FMT8BIT; + + while ((text.length = fread(buf, 1, BUFSIZ, textLogFP)) > 0) + { if (XawTextReplace(textOutputWidget, pos, pos, &text) != XawEditDone) + { fprintf(stderr,"\n*** Problem with text output. Exiting.\n"); + finalx(1); + } + pos += text.length; + } + + XawTextSetInsertionPoint(textOutputWidget, pos); + + + /* Truncate the temporary file for output by + redirecting everything from stdout there. + Also open it again for reading. + */ + if ( (freopen(textLogFN, "w", stdout) == NULL) + || (freopen(textLogFN, "r", textLogFP) == NULL) + ) + { fprintf(stderr,"\n*** Failed to access temporary file. Exiting.\n"); + finalx(1); + } +} + + + +void ClearTextOutput() +/* + The text output is cleared. +*/ +{ XawTextBlock text; + char buf[1]; + XawTextPosition pos; + + /* Get the end-of-text position, in a rather dodgy way */ + XawTextSetInsertionPoint(textOutputWidget, MAXLONG); + pos = XawTextGetInsertionPoint(textOutputWidget); + + text.firstPos = 0; + text.ptr = buf; + text.length = 0; + text.format = FMT8BIT; + + (void) XawTextReplace(textOutputWidget, 0, pos, &text); + + XawTextSetInsertionPoint(textOutputWidget, 0); +} + diff --git a/src/staden/textOutput.h b/src/staden/textOutput.h new file mode 100644 index 0000000..c8ce8e2 --- /dev/null +++ b/src/staden/textOutput.h @@ -0,0 +1,47 @@ +/* + Title: textOutput + + File: textOutput.h + Purpose: Manage a text widget displaying stdout + Last update: Thur May 17 1990 +*/ + + +/* + This module creates a text widget, and arranges for output + from stdout to be displayed. The function UpdateTextOutput() + must be called periodically to keep the display up to date. +*/ + + + + +/* ---- Imports ---- */ + +#include /* IMPORT: Widget */ + + + + +/* ---- Exports ---- */ + + +extern Widget CreateTextOutput(Widget parentWid); +/* + Create the text widget as a child of `parentWid'. + `stdout' may be redirected, but is not closed. + The display is initially empty. +*/ + + +extern void UpdateTextOutput(); +/* + Any text written to `stdout' since the last call of + UpdateTextOutput() is added to the display. +*/ + + +extern void ClearTextOutput(); +/* + The text output is cleared. +*/ diff --git a/src/staden/tman_basesDisplay.c b/src/staden/tman_basesDisplay.c new file mode 100644 index 0000000..1e9727f --- /dev/null +++ b/src/staden/tman_basesDisplay.c @@ -0,0 +1,373 @@ +/* + Title: basesDisplay + + File: basesDisplay.h + Purpose: Display the bases of a sequence on graphs + Last update: Monday 20 January 1992 +*/ + + +/* + `plotEdgeOffset' indicates the pixel position at which point 0 + occurs. The characters for bases are printed with their centres + corresponding to their locations on the plot. + + A caret is XOR drawn in the editable graph on top of (i.e. after) + all characters have been drawn. + + Changes to this module by lfw: + module displayEdBases and displayBases + added int baseNum as a parameter so that + the user could specify a baseNum via the + command line baseNum parameter or via + specifying a string to search for ( if + you specify a string to search for + ) and have that be where the caret + is originally positioned. Before this + modification, the caret was always + initially positioned at base 0. + +*/ + + + + +/* ---- Includes ---- */ + +#include "tman_basesDisplay.h" +#include "tman_display.h" +#include "tman_context.h" + +#include "Graph.h" +#include "seq.h" /* IMPORT: Seq, NULLSeq, getNPoints */ + +#include /* IMPORT: toupper */ + +#include +#include +#include +#include +#include +#include +#include + + + + +/* ---- Constants ---- */ + + + +/* ---- Statics ---- */ + +/* Current sequence information */ + +/* Font information */ +static Dimension charWidth; +static Dimension halfCharWidth; +static Dimension charVOffset; /* Of char baseline from top of graph=ascent+1 */ + +static GC Agc, Cgc, Ggc, Tgc; + +/* ---- Position translation ---- */ + +/* + Convert a value from the `point-in-the-sequence' to the + `x-pixel-on-the-graph' scale, and vice-versa, given that + `plotWidth' holds the current pixel width of the graph which + starts `plotEdgeOffset' from either edge of the graph and + `NPoints' gives the current point width. + Fractional results are truncated - for pixelToPoint this may + mean a point one too low is indicated, but a point too high is + never indicated. +*/ + + +static int pointToPixel(DisplayContext *dc, int point) +{ return(((point * dc->plotWidth) / (dc->NPoints-1)) + dc->plotEdgeOffset); +} + + +int pixelToPoint(DisplayContext *dc, int pixel) +{ return(((pixel-dc->plotEdgeOffset) * (dc->NPoints-1)) / dc->plotWidth); +} + + +static int leftCutoffPoint(DisplayContext *dc, int baseNum) +/* + Return the point corresponding to a left cutoff at `baseNum'. +*/ +{ int pL, pR; + + pL = (baseNum == 0) ? 0 : baseNumToPoint(dc->seq, OrigBases, baseNum-1); + pR = (baseNum == 0) ? 0 : baseNumToPoint(dc->seq, OrigBases, baseNum); + + return((pR==NULLPoint)?pL:(pL+pR)/2); +} + + +static int rightCutoffPoint(DisplayContext *dc, int baseNum) +/* + Return the point corresponding to a right cutoff at `baseNum'. +*/ +{ int NorigBases = getNBases(dc->seq, OrigBases); + int pL, pR; + + pL = (baseNum == 0) + ? dc->NPoints-1 + : baseNumToPoint(dc->seq, OrigBases, NorigBases-baseNum); + pR = (baseNum == 0) + ? dc->NPoints-1 + : baseNumToPoint(dc->seq, OrigBases, NorigBases-baseNum-1); + + return((pL+pR)/2); +} + + + + +/* ---- Internal routines ---- */ + + +static void writeBase(DisplayContext *dc, Widget wid, int baseNum) +{ + char base = getBase(dc->seq, OrigBases, baseNum); + int point = baseNumToPoint(dc->seq, OrigBases, baseNum); + GC gc; + + /* Get the correct GC */ + switch (base) + { case 'A': gc=Agc; break; + case 'C': gc=Cgc; break; + case 'G': gc=Ggc; break; + case 'T': gc=Tgc; break; + default: gc=Ggc; break; + } + + XDrawImageString(XtDisplay(wid), + XtWindow(wid), + gc, + pointToPixel(dc,point)-halfCharWidth, charVOffset, + &base, 1); +} + + + + + +/* ---- Callbacks ---- */ + + +static void exposeCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ Region region = (Region) call_data; + XRectangle rect; + int x0, xN; /* Affected region: pixels */ + int p0, pN; /* Affected region: points */ + int b, b0, bN; /* Affected region: bases */ + int leftCutoffP, rightCutoffP; + int NBases; + int whichBases; + DisplayContext *dc = widgetToDisplayContext(XtParent(XtParent(XtParent(wid)))); + + if (dc->seq == NULLSeq) return; + + leftCutoffP = leftCutoffPoint(dc,dc->leftCutoff); + rightCutoffP = rightCutoffPoint(dc,dc->rightCutoff); + NBases = dc->seq->NorigBases; + whichBases = OrigBases; + + + /* + `region' gives us the exposed graph region. + XClipBox will return a bounding rectangle, in pixels. + */ + XClipBox(region, &rect); + x0 = rect.x; + xN = rect.x+rect.width; + + + + /* Convert to affected points after trimming to the plot proper */ + p0=pixelToPoint(dc,(x0plotEdgeOffset) ?dc->plotEdgeOffset :x0); + pN=pixelToPoint(dc,(xN>dc->plotWidth+dc->plotEdgeOffset)?dc->plotWidth+dc->plotEdgeOffset:xN); + if (pNNPoints-1) pN++; + + + /* + Now draw the affected bases. + */ + + /* The first base is to the left of the exposed region */ + b0 = pointToBaseNum(dc->seq, whichBases, p0); + b0 = (b0 == NULLBaseNum) ? NBases-1 + : (b0>0) ? b0-1 + : 0; + /* The last base is to the right of the exposed region */ + bN = pointToBaseNum(dc->seq, whichBases, pN); + bN = (bN == NULLBaseNum) ? NBases-1 : bN; + + if (b0leftCutoff) + /* + Some of the bases are in the left cutoff area. + Draw them, the edited ones with the dim background. + */ + { + for (b=b0; b<=dc->leftCutoff-1; b++) writeBase(dc,wid, b); + } + + if (bN>=dc->leftCutoff && b0-dc->seq->NorigBases-1rightCutoff) + /* + Some of the exposed region is in the middle area. + Draw the bases. + */ + { int firstBase = (b0leftCutoff) ? dc->leftCutoff : b0; + int lastBase = (bN>dc->seq->NorigBases-1-dc->rightCutoff) + ? dc->seq->NorigBases-1-dc->rightCutoff + : bN; + + for (b=firstBase; b<=lastBase; b++) writeBase(dc, wid, b); + } + + if (bN>dc->seq->NorigBases-1-dc->rightCutoff) + /* + Some of the exposed region is in the right cutoff area. + Repaint it with dimmed background. + */ + { + /* Plot the affected bases */ + for (b=dc->seq->NorigBases-dc->rightCutoff; b<=bN; b++) writeBase(dc, wid, b); + } + + +} + + + + + +/* ---- Exports ---- */ + + + + +Widget createBasesDisplay(DisplayContext *dc, Widget parentWid, Widget fromVertWid, + Dimension width) +/* + Create the bases display within the Form widget `parent', + with the XtNfromVert constraint `fromVertWid'. The initial + width is `width'. Return the (lower) widget constructed. + No bases are initially displayed. +*/ +{ Arg args[10]; + int nargs; + + dc->seq = NULLSeq; + dc->graphWidth = width; + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNwidth, dc->graphWidth); nargs++; + dc->origSeqWid = XtCreateManagedWidget("origSeq", graphWidgetClass, + parentWid, args, nargs); + XtAddCallback(dc->origSeqWid, XtNexposeCallback, exposeCallback, NULL); + + /* + Get font information. + The text graphs are set to be two pixels higher than + (ascent+descent). Characters are then plotted with + a vertical offset one more than the ascent. + */ + { XFontStruct *fontStruct; + + nargs = 0; + XtSetArg(args[nargs], XtNfont, &fontStruct); nargs++; + XtGetValues(dc->origSeqWid, args, nargs); + charWidth = fontStruct->max_bounds.width; + halfCharWidth = fontStruct->max_bounds.width/2; + charVOffset = fontStruct->ascent+1; + dc->plotEdgeOffset = halfCharWidth+2; + dc->plotWidth = dc->graphWidth-(2*dc->plotEdgeOffset); + dc->graphHeight = fontStruct->ascent + fontStruct->descent + 2; + /* Make this graph tall enough to hold it */ + nargs = 0; + XtSetArg(args[nargs], XtNheight, dc->graphHeight); nargs++; + XtSetValues(dc->origSeqWid, args, nargs); + } + + + /* + Get the GCs + */ + Agc = GraphGC1(dc->origSeqWid); + Cgc = GraphGC2(dc->origSeqWid); + Ggc = GraphGC3(dc->origSeqWid); + Tgc = GraphGC4(dc->origSeqWid); + + return dc->origSeqWid; +} + + + + +void getBasesFontInfo(DisplayContext *dc,Dimension *offset, Dimension *chWidth) +/* + In `offset' return the offset which the bases are drawn + from the left and right hand edges. The width of the characters + used is returned in `chWidth'. +*/ +{ *offset = dc->plotEdgeOffset; + *chWidth = charWidth; +} + + + + +void initBaseDisplay(Seq seq, int baseNum) +/* + Initialise the base display of sequence `seq'. +*/ +{ + DisplayContext *dc = seqToDisplayContext(seq); + + if (seq == NULLSeq) return; + dc->seq = seq; + + + /* + Get static information about the current sequence. + */ + dc->NPoints = getNPoints(dc->seq); + getCutoffs(dc->seq, &dc->leftCutoff, &dc->rightCutoff); + + +} + + + +void setBasesWidth(DisplayContext *dc, Dimension width) +/* + Set the width of the current sequence bases display. +*/ +{ Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNwidth, width); nargs++; + XtSetValues(dc->origSeqWid, args, nargs); + + dc->graphWidth = width; + dc->plotWidth = dc->graphWidth-(2*dc->plotEdgeOffset); + + /* + Clear the graphs. + */ + if (XtIsRealized(dc->origSeqWid)) + XClearWindow(XtDisplay(dc->origSeqWid), XtWindow(dc->origSeqWid)); +} + + + + + + diff --git a/src/staden/tman_basesDisplay.h b/src/staden/tman_basesDisplay.h new file mode 100644 index 0000000..ac542fa --- /dev/null +++ b/src/staden/tman_basesDisplay.h @@ -0,0 +1,65 @@ +/* + Title: basesDisplay + + File: basesDisplay.h + Purpose: Display the bases of a sequence on graphs + Last update: Monday 20 January 1992 +*/ + + +/* +*/ + + + + +/* ---- Includes ---- */ + +#include "seq.h" /* IMPORT: Seq */ +#include "tman_context.h" + +#include /* IMPORT: Widget */ + + + + +/* ---- Exports ---- */ + + +extern Widget createBasesDisplay(DisplayContext *dc,Widget parentWid, Widget fromVertWid, + Dimension width); +/* + Create the bases display within the Form widget `parent', + with the XtNfromVert constraint `fromVertWid'. The initial + width is `width'. Return the (lower) widget constructed. + No bases are initially displayed. +*/ + + +extern void getBasesFontInfo(DisplayContext *dc, Dimension *offset, Dimension *chWidth); +/* + In `offset' return the offset which the bases are drawn + from the left and right hand edges. The width of the characters + used is returned in `chWidth'. +*/ + + +extern void initBaseDisplay(Seq seq, int baseNum); +/* + Initialise the base display of sequence `seq'. +*/ + + +extern void setBasesWidth(DisplayContext *dc, Dimension width); +/* + Set the width of the current sequence bases display. +*/ + + +extern int pixelToPoint(DisplayContext *dc,int pixel); +/* + Given `pixel' on the bases display, return the corresponding point. + Fractional results are truncated - this may mean a point one too + low is indicated, but a point too high is never indicated. +*/ + diff --git a/src/staden/tman_context.c b/src/staden/tman_context.c new file mode 100644 index 0000000..83fa88b --- /dev/null +++ b/src/staden/tman_context.c @@ -0,0 +1,156 @@ +/* + Change log: + 14 August 1991 + add catchloop to destroyContexts, and loosen conditional + in destroyDisplayContext from "if (dc != NULL && dc->seq != NULL)" + to "if (dc != NULL)" + + 20 January 1992 + New routine nameToDisplayContext added + +*/ + + + +#include "seq.h" +#include "tman_context.h" + +#include +#include +#include +#include + + +static DisplayContext contexts[MAXCONTEXTS]; +static contextList[MAXCONTEXTS]; +static int contextCount; + +void initialiseDisplayContexts() +/* +** null all contexts +*/ +{ + int i; + + contextCount = 0; + for (i=0; imainFormWid); + XawFormDoLayout(parent,False); + + for (i=0; imainFormWid); + XtDestroyWidget(dc->mainFormWid); + freeSeq(dc->seq); + dc->seq = NULL; + dc->mainFormWid = NULL; + + for (j=i+1; j + +#define MAXCONTEXTS 4 +typedef struct { + Seq seq; + Widget viewportWid; + Widget vpFormWid; + Widget traceWid; + Widget origSeqWid; + Widget mainFormWid; + Widget gadgetsWid; + + Dimension minGraphWidth; /* All characters displayed + (depends on width of parent viewport) */ + Dimension maxGraphWidth; /* Bases spaced out with `MaxMagCharSpace' + blanks (depends on char size and + NorigBases) */ + Dimension graphWidth; /* Current width of the graph */ + int NPoints; + int maxTraceVal; + Dimension traceWidth; /* Current width of the trace graph */ + Dimension traceHeight; /* Current height of the trace graph */ + Dimension plotEdgeOffset; + Dimension plotWidth; /* Current width of the plot + = graphWidth - 2*plotEdgeOffset */ + int leftCutoff, rightCutoff; + Dimension graphHeight; + float scaleFactor; + char traceName[FILE_NAME_LENGTH]; /* name of the trace file */ + +} DisplayContext, *DisplayContextPtr; + +extern void initialiseDisplayContexts(); +/* +** clear the slate +*/ + +extern DisplayContext *widgetToDisplayContext (Widget w); +/* +** Given a widget, return the display context containing it +*/ + +extern DisplayContext *nameToDisplayContext (char *name); +/* +** Given a trace name, return the display context containing it +*/ + +extern DisplayContext *seqToDisplayContext (Seq s); +/* +** Given a widget, return the display context containing it +*/ + +extern DisplayContext *getFreeDisplayContext (); +/* +** Find a free display context +** If none, use oldest +*/ + +extern void destroyDisplayContext (DisplayContext *dc); +/* +** Do away with a single context +*/ + +extern void destroyContexts (); +/* +** Do away with all contexts +*/ + +extern DisplayContext * getLastDisplayContext(); +/* +** return last context in list +*/ + +#endif /* _context_h */ diff --git a/src/staden/tman_display.c b/src/staden/tman_display.c new file mode 100644 index 0000000..c6b42db --- /dev/null +++ b/src/staden/tman_display.c @@ -0,0 +1,426 @@ +/* + Title: tman_display + + File: tman_display.c + Purpose: Sequence display for trace manager + Last update: Monday 20 January 1992 +*/ + + +/* + The display is created by a call to CreateDisplay. It principally + consists of two components: the first, created by a call to + createBasesDisplay displays the original and edited bases, the + second, created by a call to createTracesDisplay, displays the + traces. + + The viewport widget does not itself hand out resize events, so we + create a dummy (graph) widget in the same patch, connected in the + same way to the walls of the form, to collect resize events and + allow us to adjust scalings for the graphs within the viewport. + This is slightly dodgy and should be redone some other way. + + Changes to this program by lfw: + module displaySeq + Added the parameter mag to the parameters + passed to the subroutine + Changed to default magnification to 30% + by changing the call to setGraphWidth + to be 30 rather than -1. And by + allowing the call to setGraphWidth + to be specified by the user which + comes from the main calling program + via the mag parameter + + 9 July: New trace title parameter in createDisplay + 20 January: reposition trace if already managed +*/ + + + + +/* ---- Includes ---- */ + +#include "tman_display.h" +#include "tman_traceDisplay.h" /* IMPORT: createTraceDisplay, displayTrace, + unDisplayTrace, setTraceWidth */ +#include "tman_basesDisplay.h" /* IMPORT: createBasesDisplay, displayBases, + unDisplayBases, setBasesWidth */ +#include "tman_context.h" /* IMPORT: DisplayContext getFreeDisplayContext */ +#include "tman_gadgets.h" +#include "Graph.h" +#include "seq.h" /* IMPORT: Seq, NULLSeq, getNPoints */ +#include "edUtils.h" +#include "tagUtils.h" /* IMPORT: FILE_NAME_LENGTH */ + +#include +#include /* IMPORT: isgraph */ + +#include +#include +#include +#include +#include +#include +#include +#include + + + + +/* ---- Constants ---- */ + + +#define MaxDimension 32767 /* By definition, 2^16-1 */ +#define MaxMagCharSpace 5 /* At maximum magnification, the distance in + characters between each base */ +#define ViewportFudge 10 /* A guess of the space required within the + viewport for the graph to fit */ + + + + +/* ---- Statics ---- */ + +/* Current sequence information */ + + + + +/* ---- Internal routines ---- */ + + + + +static void setGraphWidth(DisplayContext *dc,int percent) +/* + Set the widths of the graphs to be `percent' between + the minimum and maximum widths. + The current ``centre of interest'' is maintained. +*/ +{ int nargs; + Arg args[10]; + float centre, topOfThumb, shown; + Widget hScrollWid = XtNameToWidget(dc->viewportWid, "horizontal"); + + /* + The ``centre of interest'' is the middle of the displayed + graph. Percentagewise, this is given by `topOfThumb+show/2'. + */ + nargs=0; + XtSetArg(args[nargs], XtNtopOfThumb, &topOfThumb); nargs++; + XtSetArg(args[nargs], XtNshown, &shown); nargs++; + XtGetValues(hScrollWid, args, nargs); + centre = topOfThumb+shown/2; + + /* + Set the displays to the required width + */ + dc->graphWidth = ((dc->maxGraphWidth-dc->minGraphWidth) * percent)/100 + dc->minGraphWidth; + XawFormDoLayout(dc->vpFormWid, False); + nargs = 0; + XtSetArg(args[nargs], XtNwidth, dc->graphWidth); nargs++; + setBasesWidth(dc,dc->graphWidth); + setTraceWidth(dc,dc->graphWidth); + XawFormDoLayout(dc->vpFormWid, True); + + /* + We have changed the size of the form widget within the viewport + widget. The viewport does not define where exactly the form + will now sit, so we manually reset the ``centre of interest'', + keeping `shown' at its (new) value. + */ + nargs=0; + XtSetArg(args[nargs], XtNtopOfThumb, &topOfThumb); nargs++; + XtSetArg(args[nargs], XtNshown, &shown); nargs++; + XtGetValues(hScrollWid, args, nargs); + topOfThumb = centre-shown/2; + XawScrollbarSetThumb(hScrollWid, topOfThumb, shown); + XtCallCallbacks(hScrollWid, XtNjumpProc, &topOfThumb); + +} + + + + + + + + + +/* ---- Callbacks ---- */ + +#ifdef notdef + +static void resizeCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ Dimension width; + int mag; + Arg args[10]; + int nargs; + + DisplayContext *dc = widgetToDisplayContext(XtParent(wid)); + + nargs = 0; + XtSetArg(args[nargs], XtNwidth, &width); nargs++; + XtGetValues(dc->viewportWid, args, nargs); + + /* Back calculate what the current magnification is */ + mag = ((dc->graphWidth-dc->minGraphWidth)*100) / (dc->maxGraphWidth-dc->minGraphWidth); + + /* Adjust the minimum magnification */ + dc->minGraphWidth = width-ViewportFudge; + dc->graphWidth = dc->minGraphWidth; + + /* Set us to the old magnification, but with the new dimensions */ + setGraphWidth(dc,mag); +} + +#endif + + + + + +/* ---- Exports ---- */ + + +DisplayContext *createDisplay(Widget parentWid, Widget fromVertWid, char* traceName, char *traceTitle) +/* + Create the display within the Form widget `parentWid' + with the XtNfromVert constraint `fromVertWid'. + No sequence is initally displayed. +*/ +{ + EdStruct *xx = intToEdStruct(0); + + Widget basesWid; + Arg args[10]; + int nargs; + Dimension charWidth; /* As used by basesDisplay */ + Dimension plotEdgeOffset; + Dimension vpWidth = 0; + Dimension vpHeight = 0; + + DisplayContext *dc = getFreeDisplayContext(); + + strncpy(dc->traceName,traceName,FILE_NAME_LENGTH); + + if (fromVertWid != NULL) { + DisplayContext *ref = widgetToDisplayContext(fromVertWid); + + nargs = 0; + XtSetArg(args[nargs], XtNwidth, &vpWidth); nargs++; + XtSetArg(args[nargs], XtNheight, &vpHeight); nargs++; + XtGetValues(ref->viewportWid , args, nargs); + } else { + /* + ** make default width to be the same as the + ** contig editor sequences widget + */ + nargs = 0; + XtSetArg(args[nargs], XtNwidth, &vpWidth); nargs++; + XtGetValues(xx->sequencesWid, args, nargs); + + } + + /* The main display */ + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + dc->mainFormWid = XtCreateWidget("form", formWidgetClass, + parentWid, args, nargs); + + + /* Other buttons */ + nargs = 0; + dc->gadgetsWid = XtCreateManagedWidget("gadgets", formWidgetClass, + dc->mainFormWid, args, nargs); + + createGadgets(dc->gadgetsWid,traceTitle); + + + /* create graph */ + nargs = 0; + XtSetArg(args[nargs], XtNforceBars, True); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, dc->gadgetsWid); nargs++; + if (vpWidth != 0) { + XtSetArg(args[nargs], XtNwidth, vpWidth); nargs++; + } + if (vpHeight != 0) { + XtSetArg(args[nargs], XtNheight, vpHeight); nargs++; + } + dc->viewportWid = XtCreateManagedWidget("viewport", viewportWidgetClass, + dc->mainFormWid, args, nargs); + + + /* + The minimum graph width is the viewport width minus a + random amount to allow for spacing between the viewport + and the graph, borders etc. + The initial graph width is this minimum width. + */ + + { + Dimension vpWidth; + + nargs = 0; + XtSetArg(args[nargs], XtNwidth, &vpWidth); nargs++; + XtGetValues(dc->viewportWid, args, nargs); + + dc->minGraphWidth = vpWidth-ViewportFudge; + dc->graphWidth = dc->minGraphWidth; + } + + + + + nargs = 0; + dc->vpFormWid = XtCreateManagedWidget("vpForm", formWidgetClass, + dc->viewportWid, args, nargs); + + + basesWid = createBasesDisplay(dc, dc->vpFormWid, NULL, dc->graphWidth); + + getBasesFontInfo(dc,&plotEdgeOffset, &charWidth); + createTraceDisplay(dc, dc->vpFormWid,basesWid,dc->graphWidth, plotEdgeOffset); + + XtManageChild(dc->mainFormWid); + + return dc; +} + + +DisplayContext *getDisplay(Widget parentWid, Widget fromVertWid, char* traceName, char *traceTitle) +/* + get a display context, create one if necessary +*/ +{ + + DisplayContext *dc; + + dc = nameToDisplayContext(traceName); + + if (dc == NULL) + dc = createDisplay(parentWid, fromVertWid, traceName, traceTitle); + + return dc; + +} + + +void displaySeq(Seq seq, int baseNum, int leftCutOff, int cutLength, int baseSpacing) +/* + Display the sequence `seq' for editing. + If baseNum is not equal to NULLBaseNum, display at 30% (or mag) + magnification centered on baseNum. +*/ +{ + + DisplayContext *dc = seqToDisplayContext(seq); + + if (seq == NULLSeq) return; + + initBaseDisplay(seq,baseNum); + initTraceDisplay(seq); + + + /* + The maximum graph width is such to allow for a `MaxMagCharSpace' + spacing between bases. + + There is a limit on Dimension sizes, noting that the graph we + generate has to fit into a Viewport. + */ + { + long maxWidth; + int NorigBases; + Dimension charWidth; /* As used by basesDisplay */ + Dimension plotEdgeOffset; + + NorigBases = getNBases(seq, OrigBases); + getBasesFontInfo(dc,&plotEdgeOffset, &charWidth); +/* + maxWidth = ((NorigBases-1) * charWidth * MaxMagCharSpace) + + (2 * plotEdgeOffset); +** +** + maxWidth = (int) ((float) ((NorigBases-1) * baseSpacing) * + (float) dc->NPoints / + (float) baseNumToPoint(seq, OrigBases,NorigBases-1) + ) + (2 * plotEdgeOffset); +*/ + if (leftCutOff > NorigBases) + leftCutOff = NorigBases; + if (leftCutOff < 0) + leftCutOff = 0; + if (leftCutOff+cutLength > NorigBases) + cutLength = NorigBases-leftCutOff; + + maxWidth = (int) ((float) ((cutLength-1) * baseSpacing) * + (float) dc->NPoints / ( + (float) baseNumToPoint(seq, OrigBases,leftCutOff+cutLength-1) - + (float) baseNumToPoint(seq, OrigBases,leftCutOff)) + ) + (2 * plotEdgeOffset); + + dc->maxGraphWidth = + (maxWidth>MaxDimension-ViewportFudge) ? MaxDimension-ViewportFudge + : (maxWidthminGraphWidth) ? dc->minGraphWidth + : maxWidth; + } + + + if (baseNum!=NULLBaseNum) + { /* Centred on baseNum, half magnification */ + Widget hScrollWid = XtNameToWidget(dc->viewportWid, "horizontal"); + float topOfThumb; + + /* Set the `centre of interest' on baseNum. Zero `shown'. */ + topOfThumb = (float) baseNumToPoint(seq, OrigBases,baseNum) / (float) dc->NPoints; + + XawScrollbarSetThumb(hScrollWid, topOfThumb, 0.0); + + } + + setGraphWidth(dc,100/*mag*/); +} + + + +void repositionSeq(Seq seq, int baseNum) +/* + Reposition the sequence `seq' at a given base number (centred) +*/ +{ + + DisplayContext *dc = seqToDisplayContext(seq); + + if (seq == NULLSeq) return; + + + if (baseNum!=NULLBaseNum) + { /* Centred on baseNum, half magnification */ + Widget hScrollWid = XtNameToWidget(dc->viewportWid, "horizontal"); + float topOfThumb; + float shown; + int nargs; + Arg args[10]; + + /* Set the `centre of interest' on baseNum. */ + nargs=0; + XtSetArg(args[nargs], XtNshown, &shown); nargs++; + XtGetValues(hScrollWid, args, nargs); + topOfThumb = (float) baseNumToPoint(seq, OrigBases,baseNum) / (float) dc->NPoints; + topOfThumb = topOfThumb - shown/2; + XawScrollbarSetThumb(hScrollWid, topOfThumb, shown); + + /* force redraw the only way we know how */ + XtCallCallbacks(hScrollWid, XtNjumpProc, &topOfThumb); + } + +} + + + + + + diff --git a/src/staden/tman_display.h b/src/staden/tman_display.h new file mode 100644 index 0000000..f4765f9 --- /dev/null +++ b/src/staden/tman_display.h @@ -0,0 +1,81 @@ +/* + Title: tman_display + + File: tman_display.h + Purpose: Sequence display for trace manager + Last update: Monday 20 January 1992 +*/ + + +/* +*/ + + + + +/* ---- Includes ---- */ + +#include "seq.h" /* IMPORT: Seq */ +#include "tman_context.h" + +#include /* IMPORT: Widget */ + + + + +/* ---- Exports ---- */ + + +extern DisplayContext *createDisplay(Widget parentWid, Widget superiorWid, char *traceName, char *traceTitle); +/* + Create the display within the Form widget `parent', + underneath `superiorWid'. + No sequence is initially displayed. +*/ + +extern DisplayContext *getDisplay(Widget parentWid, Widget superiorWid, char *traceName, char *traceTitle); +/* + Get a display context for traceName, reusing one if one for the + same name exists. + No sequence is initially displayed. +*/ + + +extern void displaySeq(Seq seq, int baseNum, int leftCutOff, int cutLength, int baseSpacing); +/* + Display the sequence `seq' for editing. + If baseNum is not equal to -1, display at half magnification + centered on baseNum. (unless the user has also set mag) +*/ + +extern void repositionSeq(Seq seq, int baseNum); +/* + Reposition the sequence `seq' at a given base number (centred) +*/ + + +extern void unDisplaySeq(); +/* + Cease displaying the current sequence (if any). +*/ + + +extern Seq displayedSeq(); +/* + Return the currently displayed sequence, or NULLSeq + if none is being displayed. +*/ + + +extern void userEvent(XEvent *eventP); +/* + Handle the (key or button) event generated in a subdisplay. +*/ + + + + + + + + diff --git a/src/staden/tman_gadgets.c b/src/staden/tman_gadgets.c new file mode 100644 index 0000000..bf9d793 --- /dev/null +++ b/src/staden/tman_gadgets.c @@ -0,0 +1,132 @@ +/* + Title: Trace manager graph gadgets + + File: tman_gadgets.c + Purpose: gadgets for the trace manager + Last update: Tuesday 9 July 1991 +*/ + + +/* +9 July 1991 SD Widened label field from 12 to 18 chars +*/ + + + + +/* ---- Includes ---- */ + +#include "tman_context.h" +#include "tman_traceDisplay.h" +#include "Graph.h" +#include +#include +#include +#include +#include +#include + +/* ---- Constants ---- */ + + +/* ---- Statics ---- */ + + + + + +/* ---- Internal routines ---- */ + + + + + +/* ---- Callbacks ---- */ + + +static void quitCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ + DisplayContext *dc=widgetToDisplayContext(XtParent(XtParent(wid))); + + destroyDisplayContext(dc); +} + + + +static void scaleUpCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ + DisplayContext *dc=widgetToDisplayContext(XtParent(XtParent(wid))); + + incScaleFactor(dc); +} + +static void scaleDownCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ + DisplayContext *dc=widgetToDisplayContext(XtParent(XtParent(wid))); + + decScaleFactor(dc); +} + + + + + + + +/* ---- Exports ---- */ + + +void createGadgets(Widget parentWid,char *traceName) +/* +*/ +{ + Widget labelWid,scaleUpWid,scaleDownWid,/*lockWid,*/quitWid; + Arg args[10]; + int nargs; + + char traceLabel[18]; + Cstr2Fstr(traceName,traceLabel,18); + traceLabel[17]='\0'; + + /* + ** A title + */ + nargs = 0; + XtSetArg(args[nargs], XtNlabel, traceLabel); nargs++; + labelWid = XtCreateManagedWidget("traceName", labelWidgetClass, + parentWid, args, nargs); + + /* for vertical scaling */ + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, labelWid); nargs++; + scaleUpWid = XtCreateManagedWidget("scaleUp", commandWidgetClass, + parentWid, args, nargs); + XtAddCallback(scaleUpWid, XtNcallback, scaleUpCallback, NULL); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, scaleUpWid); nargs++; + scaleDownWid = XtCreateManagedWidget("scaleDown", commandWidgetClass, + parentWid, args, nargs); + XtAddCallback(scaleDownWid, XtNcallback, scaleDownCallback, NULL); + + /* Other buttons */ + /* + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, scaleDownWid); nargs++; + lockWid = XtCreateManagedWidget("lock", toggleWidgetClass, + parentWid, args, nargs); + */ + + nargs = 0; + /* + XtSetArg(args[nargs], XtNfromHoriz, lockWid); nargs++; + */ + XtSetArg(args[nargs], XtNfromVert, scaleDownWid); nargs++; + quitWid = XtCreateManagedWidget("quit", commandWidgetClass, + parentWid, args, nargs); + XtAddCallback(quitWid, XtNcallback, quitCallback, NULL); + +} diff --git a/src/staden/tman_gadgets.h b/src/staden/tman_gadgets.h new file mode 100644 index 0000000..67e4c12 --- /dev/null +++ b/src/staden/tman_gadgets.h @@ -0,0 +1,27 @@ +/* + Title: graph gadgets + + File: gadgets.h + Purpose: gadgets for the graph viewport + Last update: Thursday Jan 10 1991 +*/ + + +/* +*/ + + + + +/* ---- Includes ---- */ + + +#include + + +/* ---- Exports ---- */ + + +extern void createGadgets(Widget parentWid, char *traceName); +/* +*/ diff --git a/src/staden/tman_main.c b/src/staden/tman_main.c new file mode 100644 index 0000000..d386e3c --- /dev/null +++ b/src/staden/tman_main.c @@ -0,0 +1,251 @@ +/* + Title: tman_main + + File: tman_main.c + Purpose: Main module for Trace Manager + Last update: Tuesday 14 April 1992 + + Change log: + + 9 July 1991 New trace title parameter in manageTrace, and call to + createDisplay + Fixed problem with geometry management with a sleep(1) + after widget popup. Seems to work...not sure why + + 16 Jan 1992 When trace is already present in the trace manager + reposition old trace + + 14 Feb 1992 Support SCF format traces + 14 Apr 1992 If file if SCF read in regardless of claimed format. +*/ + + +/* + +*/ + + + + +/* ---- Includes ---- */ + +#include "mystdlib.h" /* IMPORT: exit */ + +#include "tman_main.h" +#include "tman_display.h" /* IMPORT: displaySeq, unDisplaySeq */ +#include "tman_traceDisplay.h" /* IMPORT: setScaleFactor */ +#include "tman_context.h" +#include "seq.h" /* IMPORT: Seq, NULLSeq */ +#include "seqIOABI.h" /* IMPORT: readSeqABI */ +#include "seqIOALF.h" /* IMPORT: readSeqALF */ +#include "seqIOSCF.h" /* IMPORT: readSeqSCF */ +#include "seqIOPlain.h" /* IMPORT: readSeqPlain */ + + +#include +/*#include */ +#include +#include +#include +#include +#include +#include + +/* ---- definitions ---- */ +#define TMAN_STATE_DOWN 0 +#define TMAN_STATE_UP 1 + + +/* ---- Static variables ---- */ +static int tman_state = TMAN_STATE_DOWN; +static Widget traceManagerShellWidget; +static Widget mainFormWid; +static Widget buttonWid,quitWid,hideWid; +static Widget traceForm; + + +static void initialDisplayedSeq(DisplayContext *dc, + char *format, char *fn, + int baseNum, int leftCutOff, int cutLength, + int bottom, int baseSpacing) +/* + This function may be called once, after the application + has been realised, to specify a sequence to be displayed + initially. If it is not called, no sequence is initially + displayed. +*/ +{ + if (dc->seq == NULLSeq) { + /* + ** if file is in SCF read in as such regardless of `format' + */ + if (is_SCF(fn)) + dc->seq = readSeqSCF(fn); + else { + if (strcmp(format, "ABI ") == 0) + dc->seq = readSeqABI(fn); + else if (strcmp(format, "ALF ") == 0) + dc->seq = readSeqALF(fn); + else if (strcmp(format, "SCF ") == 0) + dc->seq = readSeqSCF(fn); + else + dc->seq = readSeqPlain(fn); + } + + if (dc->seq != NULLSeq) + { + int i; + /* + * YUK! + * We need to do the following so that complement_seq() works + */ + /* All ed bases are the same are orig bases */ + for (i=0;iseq->NorigBases;i++) dc->seq->edits[i] = i; + + /* The table of edits has all its entries free */ + for (i=0;iseq->edBasePos[i] = NULLPoint; + + oppInitialize(); + if (bottom) complement_seq(dc->seq); + setScaleFactor(dc,1.0); + + /* + Set the `file name' and `number of bases' labels. + */ + displaySeq(dc->seq, + ((baseNum!=NULLBaseNum) && + (baseNum>=0) && + (baseNumseq,OrigBases)) + ) + ? baseNum + : NULLBaseNum, + leftCutOff, + cutLength, + baseSpacing + ); + } + else + XBell(XtDisplay(traceManagerShellWidget), 100); + } else { + repositionSeq(dc->seq, + ((baseNum!=NULLBaseNum) && + (baseNum>=0) && + (baseNumseq,OrigBases)) + ) + ? baseNum + : NULLBaseNum + ); + + } + +} + + + +/* ---- Callbacks ---- */ +static void quitCallback(Widget widget, + XtPointer client_data, XtPointer call_data) +{ /* hide the trace manager widget */ + XtPopdown(traceManagerShellWidget); + destroyContexts(); + tman_state = TMAN_STATE_DOWN; +} + +static void hideCallback(Widget widget, + XtPointer client_data, XtPointer call_data) +{ /* hide the trace manager widget */ + tman_state = TMAN_STATE_DOWN; + XtPopdown(traceManagerShellWidget); +} + +/* ---- Exported functions ---- */ + + +void CreateTraceManager(Widget parentWid) +{ + Arg args[10]; + int nargs; + + + /* + Create the widgets + */ + traceManagerShellWidget = XtCreatePopupShell ("Traces", + topLevelShellWidgetClass, + parentWid, + NULL, (Cardinal) 0); + + mainFormWid = XtCreateManagedWidget("mainForm", formWidgetClass, + traceManagerShellWidget, + NULL, (Cardinal) 0); + + + /* + ** Box widget for buttons + */ + nargs = 0; + buttonWid = XtCreateManagedWidget("buttons", boxWidgetClass, + mainFormWid, args, nargs); + + nargs = 0; + quitWid = XtCreateManagedWidget("quit", commandWidgetClass, + buttonWid, args, nargs); + XtAddCallback(quitWid, XtNcallback, quitCallback, NULL); + + nargs = 0; + hideWid = XtCreateManagedWidget("hide", commandWidgetClass, + buttonWid, args, nargs); + XtAddCallback(hideWid, XtNcallback, hideCallback, NULL); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, buttonWid); nargs++; + traceForm = XtCreateManagedWidget("traceForm", formWidgetClass, + mainFormWid, args, nargs); + + initialiseDisplayContexts(); + tman_state = TMAN_STATE_DOWN; + +} + + +void manageTrace( + char *format, + char *rawDataFile, + int baseNum, + int leftCutOff, + int cutLength, + int complimented, + int baseSpacing, + char *traceTitle + ) +{ + DisplayContext *dc,*lastdc; + char *traceName; + + + if ((traceName=(char *)strrchr(rawDataFile,'/'))==NULL) + traceName = rawDataFile; + else + traceName++; + + lastdc = getLastDisplayContext(); + dc = getDisplay(traceForm,(lastdc==NULL)?NULL:lastdc->mainFormWid,traceName, traceTitle); + + if (tman_state == TMAN_STATE_DOWN) { + XtPopup(traceManagerShellWidget,XtGrabNone); + XSync(XtDisplay(traceManagerShellWidget),False); + /* We need to sleep here to let X perform its + ** geometry negotiation correctly. + ** It's a bit of a kludge...sorry! + */ + sleep(1); + tman_state = TMAN_STATE_UP; + } + + initialDisplayedSeq(dc, format, rawDataFile, baseNum, + leftCutOff, cutLength, complimented, baseSpacing); + + XawFormDoLayout(traceForm,True); + + +} diff --git a/src/staden/tman_main.h b/src/staden/tman_main.h new file mode 100644 index 0000000..f28d0ca --- /dev/null +++ b/src/staden/tman_main.h @@ -0,0 +1,33 @@ +/* + Title: tman_main.h + + File: tman_main.h + Last update: Tueday 9 July 1991 + + Change log: + +*/ + + +/* + This module contains the C language entry point `main' and + initialisation for the X system. +*/ + +#include + + + +/* ---- Exports ---- */ +extern void CreateTraceManager(Widget parentWid); + +extern void manageTrace( + char *format, + char *rawDataFile, + int baseNum, + int leftCutOff, + int cutLength, + int complimented, + int baseSpacing, + char *traceTitle + ); diff --git a/src/staden/tman_traceDisplay.c b/src/staden/tman_traceDisplay.c new file mode 100644 index 0000000..432595e --- /dev/null +++ b/src/staden/tman_traceDisplay.c @@ -0,0 +1,439 @@ +/* + Title: traceDisplay + + File: traceDisplay.c + Purpose: Display the trace of a sequence on a graph widget + Last update: Monday 20 January 1992 +*/ + + +/* + `plotEdgeOffset' indicates the pixel position at which point 0 + occurs. The characters for bases are printed with their centres + corresponding to their locations on the plot. + + The cutoffs are actually drawn between the positions of the + bases on either side. +*/ + + + + +/* ---- Includes ---- */ + +#include "tman_traceDisplay.h" +#include "tman_display.h" /* IMPORT: userEvent */ +#include "Graph.h" +#include "seq.h" /* IMPORT: Seq, NULLSeq, getNPoints */ +#include "values.h" /* IMPORT: M_SQRT2 */ +#include +#include +#include +#include + + + + +/* ---- Statics ---- */ + +static GC Agc, Cgc, Ggc, Tgc; +static Pixel normBackground, dimBackground; + + + + + +/* ---- Position translation ---- */ + +/* + Convert a value from the `point-in-the-sequence' to the + `x-pixel-on-the-graph' scale, and vice-versa, given that + `plotWidth' holds the current pixel width of the graph which + starts `plotEdgeOffset' from either edge of the graph and + `NPoints' gives the current point width. + Fractional results are truncated - for pixelToPoint this may + mean a point one too low is indicated, but a point too high is + never indicated. +*/ + + +static int pointToPixel(DisplayContext *dc, int point) +{ return(((point * dc->plotWidth) / (dc->NPoints-1)) + dc->plotEdgeOffset); +} + + +static int pixelToPoint(DisplayContext *dc, int pixel) +{ return(((pixel-dc->plotEdgeOffset) * (dc->NPoints-1)) / dc->plotWidth); +} + + +static int scaleTrace(DisplayContext *dc, int y) +{ + return(dc->traceHeight - + (int)(dc->scaleFactor * (float)y * (float)dc->traceHeight / dc->maxTraceVal) + ); +} + + + +static int leftCutoffPoint(DisplayContext *dc, int baseNum) +/* + Return the point corresponding to a left cutoff at `baseNum'. +*/ +{ int pL, pR; + + pL = (baseNum == 0) ? 0 : baseNumToPoint(dc->seq, OrigBases, baseNum-1); + pR = (baseNum == 0) ? 0 : baseNumToPoint(dc->seq, OrigBases, baseNum); + + return((pR==NULLPoint)?pL:(pL+pR)/2); +} + + +static int rightCutoffPoint(DisplayContext *dc, int baseNum) +/* + Return the point corresponding to a right cutoff at `baseNum'. +*/ +{ int NorigBases = getNBases(dc->seq, OrigBases); + int pL, pR; + + pL = (baseNum == 0) + ? dc->NPoints-1 + : baseNumToPoint(dc->seq, OrigBases, NorigBases-baseNum); + pR = (baseNum == 0) + ? dc->NPoints-1 + : baseNumToPoint(dc->seq, OrigBases, NorigBases-baseNum-1); + + return((pL+pR)/2); +} + + + + + +/* ---- Internal routines ---- */ + + + + +static void drawTrace(DisplayContext *dc, Widget wid, char base, int p0, int pN) +/* + Draw the `base' trace between points p0..pN (inclusive) +*/ +{ GC gc; + int pSeg, pSeg0, pSegN; +#define NSegs 500 + int traces[NSegs]; + XPoint xPoint[NSegs]; + + switch (base) + { case 'A': gc=Agc; break; + case 'C': gc=Cgc; break; + case 'G': gc=Ggc; break; + case 'T': gc=Tgc; break; + } + + + /* + The range (p0..pN) is drawn as a series of overlapping + segments (pSeg0..pSegN). + */ + pSegN = p0; + do + { int i; + + /* + This segment carries on from and including the last point + of the last segment. + */ + pSeg0 = pSegN; + pSegN = ((pSeg0+NSegs-1)<=pN) ? pSeg0+NSegs-1 : pN; + + /* Get the traces for this segment */ + getTraces(dc->seq, base, pSeg0, pSegN, traces); + + /* Translate to pixel coords and place in an XPoint array */ + pSeg = pSeg0; + i = 0; + while (pSeg<=pSegN) + { xPoint[i].x = pointToPixel(dc,pSeg); + xPoint[i].y = scaleTrace(dc,traces[i]); + pSeg++; + i++; + } + + /* Draw the segments in the XPoint array */ + XDrawLines(XtDisplay(wid), + XtWindow(wid), + gc, + xPoint, + pSegN-pSeg0+1, + CoordModeOrigin); + } while (pSegN < pN); +} + + + + + +/* ---- Callbacks ---- */ + + + + +static void exposeCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ Region region = (Region) call_data; + XRectangle rect; + int x0, xN; /* Affected region: pixels */ + int p0, pN; /* Affected region: points */ + int leftCutoffP, rightCutoffP; + DisplayContext *dc = widgetToDisplayContext(XtParent(XtParent(XtParent(wid)))); + + + + if ((dc->seq == NULLSeq) || isBasesOnly(dc->seq)) return; + + + /* + `region' gives us the exposed graph region. + XClipBox will return a bounding rectangle, in pixels. + */ + XClipBox(region, &rect); + x0 = rect.x; + xN = rect.x+rect.width; + + /* Trim it so we only consider the plot proper */ + if ((x0>dc->plotWidth+dc->plotEdgeOffset) || (xNplotEdgeOffset)) return; + x0 = (x0plotEdgeOffset) ? dc->plotEdgeOffset : x0; + xN = (xN>dc->plotWidth+dc->plotEdgeOffset) ? dc->plotWidth+dc->plotEdgeOffset : xN; + + /* Convert to affected points */ + p0 = pixelToPoint(dc,x0); + pN = pixelToPoint(dc,xN); + if (pNNPoints-1) pN++; + + leftCutoffP = leftCutoffPoint(dc,dc->leftCutoff); + rightCutoffP = rightCutoffPoint(dc,dc->rightCutoff); + + + if (p0traceWid), XtWindow(dc->traceWid), + dimBackground); + XClearArea(XtDisplay(dc->traceWid), XtWindow(dc->traceWid), + pix0, 0, + pixN-pix0, dc->traceHeight, + False); + XSetWindowBackground(XtDisplay(dc->traceWid), XtWindow(dc->traceWid), + normBackground); + } + + + if (pN>rightCutoffP) + /* + Some of the exposed region is in the right cutoff area. + Repaint it with dimmed background. + */ + { int firstP = (p0>rightCutoffP) ? p0 : rightCutoffP; + int lastP = pN; + + int pix0 = pointToPixel(dc,firstP); + int pixN = pointToPixel(dc,lastP); + + XSetWindowBackground(XtDisplay(dc->traceWid), XtWindow(dc->traceWid), + dimBackground); + XClearArea(XtDisplay(dc->traceWid), XtWindow(dc->traceWid), + pix0, 0, + pixN-pix0, dc->traceHeight, + False); + XSetWindowBackground(XtDisplay(dc->traceWid), XtWindow(dc->traceWid), + normBackground); + } + + /* + Now draw the traces. + The GC background is unused when drawing lines, so we do + not need to change it depending on whether we are in the + cutoff region or not. + */ + drawTrace(dc,wid, 'A', p0, pN); + drawTrace(dc,wid, 'C', p0, pN); + drawTrace(dc,wid, 'G', p0, pN); + drawTrace(dc,wid, 'T', p0, pN); +} + + + + + +static void resizeCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ Arg args[10]; + int nargs; + DisplayContext *dc = widgetToDisplayContext(XtParent(XtParent(XtParent(wid)))); + + /* + This routine can be called in two ways. + (a) as a callback when the trace is resized by the user + (b) indirectly when setGraphWidth alters the graph width + */ + nargs = 0; + XtSetArg(args[nargs], XtNheight, &dc->traceHeight); nargs++; + XtSetArg(args[nargs], XtNwidth, &dc->traceWidth); nargs++; + XtGetValues(wid, args, nargs); + + if (XtIsRealized(wid)) XClearWindow(XtDisplay(wid), XtWindow(wid)); +} + + + + +/* ---- Exports ---- */ + +static void redisplayTraces(DisplayContext *dc) +{ + /* + ** Clear the area and generate an expose. + */ + XClearArea(XtDisplay(dc->traceWid), XtWindow(dc->traceWid), + 0, 0, + 0, 0, + True); +} + + +void incScaleFactor(DisplayContext *dc) +{ + dc->scaleFactor *= M_SQRT2; + redisplayTraces(dc); +} + +void decScaleFactor(DisplayContext *dc) +{ + dc->scaleFactor /= M_SQRT2; + redisplayTraces(dc); +} + + +void setScaleFactor(DisplayContext *dc, float sf) +{ + dc->scaleFactor = sf; + redisplayTraces(dc); +} + + + + + +void createTraceDisplay(DisplayContext *dc, Widget parentWid, Widget fromVertWid, + Dimension width, Dimension offset) +/* + Create the trace display within the Form widget `parent', + with the XtNfromVert constraint `fromVertWid'. The initial + width is `width' and the plot is drawn `offset' from + the left and right hand edges. + No trace is initially displayed. +*/ +{ Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNwidth, width); nargs++; + dc->traceWid = XtCreateManagedWidget("trace", graphWidgetClass, + parentWid, args, nargs); + XtAddCallback(dc->traceWid, XtNexposeCallback, exposeCallback, NULL); + XtAddCallback(dc->traceWid, XtNresizeCallback, resizeCallback, NULL); + + nargs = 0; + XtSetArg(args[nargs], XtNheight, &dc->traceHeight); nargs++; + XtGetValues(dc->traceWid, args, nargs); + dc->traceWidth = width; + dc->plotWidth = dc->traceWidth-(2*dc->plotEdgeOffset); + dc->plotEdgeOffset = offset; + + dc->seq = NULLSeq; + dc->scaleFactor = 1.0; + + /* + Get the GCs + */ + Agc = GraphGC1(dc->traceWid); + Cgc = GraphGC2(dc->traceWid); + Ggc = GraphGC3(dc->traceWid); + Tgc = GraphGC4(dc->traceWid); + + /* + Get the backgrounds + */ + nargs = 0; + XtSetArg(args[nargs], XtNbackground, &normBackground); nargs++; + XtSetArg(args[nargs], XtNdimBackground, &dimBackground); nargs++; + XtGetValues(dc->traceWid, args, nargs); +} + + + + +void initTraceDisplay(Seq seq) +/* + Initialise the trace display of sequence `seq'. +*/ +{ + DisplayContext *dc = seqToDisplayContext(seq); + if (seq == NULLSeq) return; + dc->seq = seq; + + + /* + Get static information about the current sequence. + */ + dc->NPoints = getNPoints(dc->seq); + dc->maxTraceVal = getMaxTraceVal(dc->seq); + getCutoffs(dc->seq, &dc->leftCutoff, &dc->rightCutoff); +} + + + + + + +void setTraceWidth(DisplayContext *dc, Dimension width) +/* + Set the width of the current sequence trace display. +*/ +{ Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNwidth, width); nargs++; + XtSetValues(dc->traceWid, args, nargs); + + dc->traceWidth = width; + dc->plotWidth = dc->traceWidth-(2*dc->plotEdgeOffset); + + /* + Clear the graph. + */ + if (XtIsRealized(dc->traceWid)) + XClearWindow(XtDisplay(dc->traceWid), XtWindow(dc->traceWid)); +} + + + + + + + + + + diff --git a/src/staden/tman_traceDisplay.h b/src/staden/tman_traceDisplay.h new file mode 100644 index 0000000..49276c6 --- /dev/null +++ b/src/staden/tman_traceDisplay.h @@ -0,0 +1,68 @@ +/* + Title: traceDisplay + + File: traceDisplay.h + Purpose: Display the trace of a sequence on a graph widget + Last update: Monday 20 January 1992 +*/ + + +/* +*/ + + + + +/* ---- Includes ---- */ + +#include "seq.h" /* IMPORT: Seq */ +#include "tman_context.h" + +#include /* IMPORT: Widget, Dimension */ + + + + +/* ---- Exports ---- */ + + +extern void createTraceDisplay(DisplayContext *dc, Widget parentWid, Widget fromVertWid, + Dimension width, Dimension offset); +/* + Create the trace display within the Form widget `parent', + with the XtNfromVert constraint `fromVertWid'. The initial + width is `width' and the plot is drawn `plotEdgeOffset' from + the left and right hand edges. + No trace is initially displayed. +*/ + + +extern void initTraceDisplay(Seq seq); +/* + Initialise the trace display of sequence `seq'. +*/ + + +extern void setTraceWidth(DisplayContext *dc, Dimension width); +/* + Set the width of the current sequence trace display. +*/ + + +extern void incScaleFactor(); +/* + Increment vertical scaling of trace +*/ + + +extern void decScaleFactor(); +/* + Decrement vertical scaling of trace +*/ + + +extern void setScaleFactor(DisplayContext *dc, float sf); +/* + Set vertical scaling +*/ + diff --git a/src/staden/trans.c b/src/staden/trans.c new file mode 100644 index 0000000..e3257c1 --- /dev/null +++ b/src/staden/trans.c @@ -0,0 +1,35 @@ +#include +/* +** We don't want to have all the textwidget translations +** Here are the list of the ones we want. +** This is taken from Xaw/TextTr.c source +*/ +char *defaultTranslations = "\ +Right: forward-character() \n\ +Left: backward-character() \n\ +Delete: delete-previous-character() \n\ +BackSpace: delete-previous-character() \n\ +: insert-char() \n\ +: focus-in() \n\ +: focus-out() \n\ +: select-start() \n\ +: extend-adjust() \n\ +: extend-end(PRIMARY, CUT_BUFFER0) \n\ +: insert-selection(PRIMARY, CUT_BUFFER0) \n\ +: extend-start() \n\ +: extend-adjust() \n\ +: extend-end(PRIMARY, CUT_BUFFER0) \ +"; + +void my_translations(Widget w) +/* +** Use my default translations +*/ +{ + XtTranslations parsedTranslations; + + parsedTranslations = XtParseTranslationTable(defaultTranslations); + + XtUninstallTranslations(w); + XtAugmentTranslations(w,parsedTranslations); +} diff --git a/src/staden/undo.c b/src/staden/undo.c new file mode 100644 index 0000000..3275858 --- /dev/null +++ b/src/staden/undo.c @@ -0,0 +1,157 @@ +#include +#include +#include "undo.h" +#include "edUtils.h" + +UndoBucketPtr bucketStack; + +/* +** undo handling code +*/ + +static UndoBucketPtr bucketFreeList = NULL; +static UndoBucketPtr newBucket() +/* +** Create a new undo bucket for the undo stack +*/ +{ + UndoBucketPtr p; + + if (bucketFreeList == NULL) { + p = (UndoBucketPtr) malloc (sizeof(UndoBucket)); + } else { + p = bucketFreeList; + bucketFreeList = bucketFreeList->next; + } + return p; +} + +static void freeBucket(UndoBucketPtr p) +/* +** Free an undo bucket and store it in a free list +*/ +{ + p->next = bucketFreeList; + bucketFreeList = p; +} + +static UndoBucketPtr popBucket(UndoBucketPtr *stack) +/* +** Get the undo bucket that's on top of and undo stack +*/ +{ + UndoBucketPtr p; + + if (*stack == NULL) { + p = NULL; + } else { + p = *stack; + *stack = (*stack)->next; + } + return p; +} + + +static void pushBucket(UndoBucketPtr *stack,UndoBucketPtr p) +/* +** Push a bucket onto an undo stack +*/ +{ + p->next = *stack; + *stack = p; +} + +void cleanUpStack(UndoBucketPtr *stack) +/* +** free all stack buckets +*/ +{ + UndoBucketPtr p; + while ( (p = popBucket(stack)) != NULL ) + freeBucket(p); +} + +void recordEdit (UndoBucketPtr *stack, EdStruct *xx, int undoEvent, int seq, int pos, char ch, int time) +/* +** record edits in an undo bucket and stick it on a stack +*/ +{ + UndoBucketPtr p; + +#define record_multi_edits +#ifndef record_multi_edits + static int state = 0; + + if (state) { + if (undoEvent == undoMark) state = 0; + } else { + cleanUpStack(stack); + if (undoEvent == undoMark) state = 1; + } +#endif + + p = newBucket(); + if (p==NULL) { + fprintf (stderr,"error: Undo stack is full... purging\n"); + cleanUpStack(stack); + p = newBucket(); + } + if (p!=NULL) { + p->event = undoEvent; + p->sequence = seq; + p->position = pos; + p->character = ch; + p->xx = xx; + p->time = time; + pushBucket (stack,p); + } +} + +void retrieveEdit (UndoBucketPtr *stack, EdStruct **xx, int *undoEvent, int *seq, int *pos, char *ch, int *time) +/* +** Get the last edit made from the undo stack and return its details +*/ +{ + UndoBucketPtr p; + p = popBucket(stack); + if (p==NULL) { + *undoEvent = undoNullCommand; + } else { + *undoEvent = p->event; + *seq = p->sequence; + *pos = p->position; + *ch = p->character; + *xx = p->xx; + *time = p->time; + freeBucket(p); + } +} + +void cleanUpAllStacks() +/* +** free all stacks +*/ +{ + cleanUpStack(&bucketStack); +} + +int editsMade(EdStruct *xx) +/* +** 0 - no edits made +** 1 - edits made +*/ +{ + int i; + for (i=1; i<=xx->DB_gelCount; i++) + if ( DBgetFlags(xx,i)&(DB_FLAG_SEQ_MODIFIED|DB_FLAG_REL_MODIFIED|DB_FLAG_TAG_MODIFIED) ) + return 1; + return 0; +} + +int get_uniq_id() +{ + static int count = 0; + + return ++count; + +} diff --git a/src/staden/undo.h b/src/staden/undo.h new file mode 100644 index 0000000..1f27d02 --- /dev/null +++ b/src/staden/undo.h @@ -0,0 +1,36 @@ +#ifndef _undo_h +#define _undo_h + +#include "edUtils.h" + +enum undoEvents { + undoDelete=0, + undoInsert, + undoReplace, + undoExtend, + undoUnextend, + undoNullCommand, + undoMark + }; + +typedef struct bnode *UndoBucketPtr; + +typedef struct bnode{ + int event; + int sequence; + int position; + int character; + struct bnode *next; + EdStructPtr xx; + int time; + } UndoBucket; + +extern UndoBucketPtr bucketStack; + +extern void cleanUpStack(UndoBucketPtr *stack); +extern void recordEdit (UndoBucketPtr *stack, EdStruct *xx, int undoEvent, int seq, int pos, char ch, int time); +extern void retrieveEdit (UndoBucketPtr *stack, EdStruct **xx, int *undoEvent, int *seq, int *pos, char *ch, int *time); +extern void cleanUpAllStacks(); +extern int editsMade(EdStruct *xx); +extern int get_uniq_id(); +#endif /* _undo_h */ diff --git a/src/staden/userface.c b/src/staden/userface.c new file mode 100644 index 0000000..f9d45cc --- /dev/null +++ b/src/staden/userface.c @@ -0,0 +1,499 @@ +/*-*-c-*-*/ +/* + * Routines to deal with the user interface in non X versions of the programs. + * C routines provided: + * getint, getfloat, yesno, yesono, gtstr, radion, checkn, getopt, showfi, + * showfu, errom, busy, bpause. + * FORTRAN routines provided: + * GETINT, GETRL, GETRLS, YESNO, YESONO, GTSTR, GETSTR, RADION, CHECK4, + * GETOPT, SHOWFI, SHOWFU, ERROM, BUSY, BPAUSE. + */ + +#include +#include +#include +#include +#include +#include "userface.h" +#include "helpnmenu.h" +#include "nxhelpmenu.h" + +/*--------------------------------------------------------------------------*\ +|* *| +|* Routines only internally used by userface.c. *| +|* *| +\*--------------------------------------------------------------------------*/ + +static char rdbuf[256]; +/* + * Reads a string from stdin storing (excluding newline at end) in buf. + * Args: + * buf: where to store string + * len: size of buf. + * Returns: + * length of string (0 for null str), + * -1 for help '?' (handled by this func.) + * -2 for quit '!', + * -3 for error (fgets() failed). + */ +static size_t rdstr(char *buf, size_t len) { + size_t l = 0; + + *buf = '\0'; + + if (len == 0) + return 0; + +#ifdef notdef + if (fgets(buf, len+2, stdin) == NULL) { + fputs("No input available!", stderr); + (void)fflush(stderr); + return -3; + } + + /* remove trailing newline */ + l = strlen(buf)-1; + /* + * A bit silly checking - if it's not got a newline on the end then + * something is wrong (we'll get it on the next read!) + */ + if (buf[l] == '\n') + buf[l]='\0'; +#endif + /* + * We use our own getline code as fgets can cause some major hassles. + * Firstly if you wish to read 10 characters then you need to tell fgets + * to read 11. Then fgets is stubborn and only reads 10 of the 11 and + * leaves the newline on the input. It can be fixed by reading 12 of + * course - but it's a bit hacky! + */ + for(;;) { + int c = getchar(); + + if (c == EOF) { + fputs("No input available!", stderr); + (void)fflush(stderr); + return -3; + } + + if (c == '\n') + break; + buf[l++] = c; + } + buf[l] = '\0'; + + if (l > 0) + if (*buf == '?') { + /* if already in interactive help mode then 'press 1' */ + if (query_opt() == -2) { + buf[0] = '1'; + buf[1] = '\0'; + return 1; + } + switch(l) { + default: + if (buf[1] == '?') { + ihelp(); + break; + } else if (query_opt() == -1) { + help2(atoi(&buf[1])); + break; + } + case 1: + help(); + } + return -1; + } else if (*buf == '!') + return -2; + + return l; +} + +/* + * Reads an integer from stdin. Note that '6G' returns '6'. + * Args: + * status: returned status indicating validity of returned integer. + * >1 = success + * 0 = null entry + * -1 = re ask question (help called or non number typed in) + * -2 = quit requested + * -3 = read error. + * Returns: + * the integer read (if valid) or 0 otherwise. + */ +static int rdint(int *status) { + int num; + char *end; + + *status = rdstr(rdbuf, sizeof(rdbuf)); + + if (*status > 0) { + num = (int)strtol(rdbuf, &end, 10); + while (*end == ' ' || *end == '\t') + end++; + return *end ? -1 : num; + } else + return 0; +} + +/* + * Reads an float from stdin. Note that '6G' returns '6'. + * Args: + * status: returned status indicating validity of returned float. + * >1 = success + * 0 = null entry + * -1 = re ask question (help called or non number typed in) + * -2 = quit requested + * -3 = read error. + * Returns: + * the float read (if valid) or 0 otherwise. + */ +static float rdfloat(int *status) { + float num; + char *end; + + *status = rdstr(rdbuf, sizeof(rdbuf)); + + if (*status > 0) { + num = (float)strtod(rdbuf, &end); + while (*end == ' ' || *end == '\t') + end++; + return *end ? -1 : num; + } else + return 0; +} + +/*--------------------------------------------------------------------------*\ +|* *| +|* C interface routines *| +|* *| +\*--------------------------------------------------------------------------*/ + +/* + * Reads an integer in a given range from stdin. + * Args: + * minval: lower end of range (inclusive) + * maxval: upper end of range (inclusive) + * defval: default integer + * prompt: question to hassle user with + * status: returned status on validity of returned value + * 0 = ok + * -2 = quit + * -3 = error + * Returns: + * minval <= int <= maxval + */ +int getint(int minval, int maxval, int defval, char *prompt, int *status) { + int val; + + do { + printf(" ? %s (%d-%d) (%d) =", prompt, minval, maxval, defval); + (void)fflush(stdout); + val = rdint(status); + } while (*status == -1 || (*status > 0 && (valmaxval))); + + if (*status == 0) + return defval; + else if (*status > 0) + return *status = 0, val; + else + return 0; +} + +/* + * Reads a float in a given range from stdin. + * Args: + * minval: lower end of range (inclusive) + * maxval: upper end of range (inclusive) + * defval: default float + * prompt: question to hassle user with + * status: returned status on validity of returned value + * 0 = ok + * -2 = quit + * -3 = error + * precision: how accurate to display the range and default values. + * Returns: + * minval <= float <= maxval + */ +float getfloat(float minval, float maxval, float defval, char *prompt, + int *status, int precision) { + float val; + + do { + printf(" ? %s (%.*f-%.*f) (%.*f) =", prompt, precision, minval, + precision, maxval, precision, defval); + (void)fflush(stdout); + val = rdfloat(status); + } while (*status == -1 || (*status > 0 && (valmaxval))); + + if (*status == 0) + return defval; + else if (*status > 0) + return *status = 0, val; + else + return 0; +} + +/* + * Prompts the user with a yes/no question (defaults to yes). + * Args: + * prompt: what to ask. + * Returns: + * 0 = yes + * 1 = no + * -1 = cancel + */ +int yesno(char *prompt) { + int answer, val; + + do { + printf(" ? %s (y/n) (y) = ", prompt); + (void)fflush(stdout); + val = rdstr(rdbuf, sizeof(rdbuf)); + if (val == 0) + answer = 0; /* default yes */ + else if (*rdbuf == 'y' || *rdbuf == 'Y') + answer = 0; + else if (*rdbuf == 'n' || *rdbuf == 'N') + answer = 1; + else + answer = -1; + } while (val > -2 && answer < 0); + + if (val >= 0) + return answer; + else /* val == -2 (quit) || val == -3 (err) */ + return -1; +} + +/* + * Reads in a string from stdin. + * Args: + * prompt: obvious + * defval: default string (if user types in nothing) + * out : where to store the actual string read. + * outlen: sizeof(out) + * Returns: + * -1 : cancel + * 0 : ok + * 1 : ok, but used default (blank string if no default) + */ +int gtstr(char *prompt, char *defval, char *out, size_t outlen) { + size_t l; + + do { + if (defval) + printf(" Default %s=%s\n", prompt, defval); + printf(" ? %s=", prompt); + (void)fflush(stdout); + + l = rdstr(out, outlen); + switch (l) { + case -3: + case -2: + *out = '\0'; + return -1; + case 0: + if (defval) { + l = strlen(defval); + strncpy(out, defval, (l>outlen)?outlen:l); + } else + *out = '\0'; + return 1; + case -1: + /* help called */ + break; + default: + return 0; + } + } while (1); +} + +/* + * Displays a menu (analogue of X radio buttons) of options and asks + * for a selection. An 'X' is displayed next to the default option. + * Args: + * title : short description of menu + * options: list of options to display + * numopts: how many options to display + * def : default option to chose. + * Returns: + * -1 = cancel + * otherwise the option number selected. + */ +int radion(char *title, char **options, int numopts, int def) { + int i, status, ret; + + do { + printf(" %s\n", title); + /* display options and prompt for selection */ + for (i=0; i OK + * IOK=1 => Cancel + */ +{ + char prompt[256]; + int status; + + Fstr2Cstr(PROMPT_p, PROMPT_l, prompt, (int_fl)sizeof(prompt)); + + *VALUE_p = getint((int)*MININ_p, (int)*MAXIN_p, (int)*DEF_p, + prompt, &status); + *IOK_p = (int_f)((status < 0)? 1:0); + + return; +} + +void getrl_x(float *MININ_p, + float *MAXIN_p, + float *DEF_p, + char *PROMPT_p, + float *VALUE_p, /* out: result */ + int_f *KBIN_p, + int_f *KBOUT_p, + int_f *IHELPS_p, + int_f *IHELPE_p, + char *HELPF_p, + int_f *IDEVH_p, + int_f *IOK_p, + int_fl PROMPT_l, + int_fl HELPF_l) +/* + * Prompt for a real in the range *MININ_p <= i <= *MAXIN_p + * The prompt is printed using format F8.2 + * IOK=0 => OK + * IOK=1 => Cancel + */ +{ + char prompt[256]; + int status; + + Fstr2Cstr(PROMPT_p, PROMPT_l, prompt, (int_fl)sizeof(prompt)); + + *VALUE_p = getfloat(*MININ_p, *MAXIN_p, *DEF_p, prompt, &status, 2); + *IOK_p = (int_f)((status < 0)? 1:0); + + return; +} + +void getrls_x(float *MININ_p, + float *MAXIN_p, + float *DEF_p, + char *PROMPT_p, + float *VALUE_p, /* out: result */ + int_f *KBIN_p, + int_f *KBOUT_p, + int_f *IHELPS_p, + int_f *IHELPE_p, + char *HELPF_p, + int_f *IDEVH_p, + int_f *IOK_p, + int_fl PROMPT_l, + int_fl HELPF_l) +/* + * Prompt for a real in the range *MININ_p <= i <= *MAXIN_p + * The prompt is printed using format F8.2 + * IOK=0 => OK + * IOK=1 => Cancel + */ +{ + char prompt[256]; + int status; + + Fstr2Cstr(PROMPT_p, PROMPT_l, prompt, (int_fl)sizeof(prompt)); + + *VALUE_p = getfloat(*MININ_p, *MAXIN_p, *DEF_p, prompt, &status, 10); + *IOK_p = (int_f)((status < 0)? 1:0); + + return; +} + +void yesno_x(int_f *ANSWER_p, + char *PROMPT_p, + int_f *IHELPS_p, + int_f *IHELPE_p, + char *HELPF_p, + int_f *IDEVH_p, + int_f *KBIN_p, + int_f *KBOUT_p, + int_fl PROMPT_l, + int_fl HELPF_l) +/* + * Prompt the user with *PROMPT_l and get a YES or NO response. + * ANSWER=-1 => Cancel + * ANSWER=0 => YES + * ANSWER=1 => NO + */ +{ + char prompt[256]; + + Fstr2Cstr(PROMPT_p, PROMPT_l, prompt, (int_fl)sizeof(prompt)); + *ANSWER_p = (int_f)yesno(prompt); +} + +void yesono_x(int_f *CHOICE_p, + char *P1_p, + char *P2_p, + int_f *IHELPS_p, + int_f *IHELPE_p, + char *HELPF_p, + int_f *IDEVH_p, + int_f *KBIN_p, + int_f *KBOUT_p, + int_fl P1_l, + int_fl P2_l, + int_fl HELPF_l) +/* + * If CHOICE is 0, present a YESNO dialogue with prompt P1. + * If CHOICE is 1, present a YESNO dialogue with prompt P2. + */ +{ + char prompt1[256], prompt2[256]; + + Fstr2Cstr(P1_p, P1_l, prompt1, (int_fl)sizeof(prompt1)); + Fstr2Cstr(P2_p, P2_l, prompt2, (int_fl)sizeof(prompt2)); + + *CHOICE_p = (int_f)yesono(*CHOICE_p, prompt1, prompt2); +} + +void gtstr_x(char *P_p, /* prompt */ + char *STRING_p, /* default value (if *LENGTH_p > 0) */ + char *NEW_p, /* out: dialogue result */ + int_f *LENGTH_p, /* in: significant length of *STRING_p + out: significant length of *NEW_p */ + int_f *KBOUT_p, + int_f *KBIN_p, + int_f *INFLAG_p, /* out: status */ + int_fl P_l, + int_fl STRING_l, + int_fl NEW_l) +/* + * Prompt with P for a string, default value STRING. + * Return new string in NEW. + * INFLAG=0 => OK + * INFLAG=1 => Help + * INFLAG=2 => Cancel, LENGTH=1 + * INFLAG=3 => LENGTH=0 and NEW full of blanks (ie default) + */ +{ + char question[256], def[256]; + char *defptr; + int ret; + + Fstr2Cstr(P_p, P_l, question, (int_fl)sizeof(question)); + if (*LENGTH_p > 0) { + Fstr2Cstr(STRING_p, STRING_l, def, (int_fl)sizeof(def)); + defptr = def; + } else + defptr = 0; + + ret = gtstr(question, defptr, NEW_p, (size_t)((NEW_l==1)?40:NEW_l)); + switch(ret) { + case -1: + *INFLAG_p = 2; + *LENGTH_p = 1; + return; + case 0: + *INFLAG_p = 0; + *LENGTH_p = (int_f)strlen(NEW_p); + Cstr2Fstr(NEW_p, NEW_p, NEW_l); + return; + case 1: + *INFLAG_p = 3; + *LENGTH_p = 0; + } +} + +void getstr_x(char *P_p, /* prompt */ + char *STRING_p, /* default value (if *LENGTH_p > 0) */ + char *NEW_p, /* out: dialogue result */ + int_f *MAXSTR_p, /* array length of *STRING_p and *NEW_p */ + int_f *LENGTH_p, /* in: significant length of *STRING_p + out: significant length of *NEW_p */ + int_f *KBOUT_p, + int_f *KBIN_p, + int_f *INFLAG_p, /* out: status */ + int_fl P_l) +/* + * Present a dialogue requesting a string result with the given prompt + * and default. + * + * In the Fortran, P is a character string, so it passes both *P_p + * and P_l. STRING and NEW are character arrays, so we are merely + * given *STRING_p and *NEW_p. + */ +{ + gtstr_x(P_p, + STRING_p, + NEW_p, + LENGTH_p, + KBOUT_p, + KBIN_p, + INFLAG_p, + P_l, + *MAXSTR_p, + *MAXSTR_p); +} + +void radion_x(char *TITLE_p, + char *PROMPT_p, /* Actually: *PROMPT_p[*NB_p] */ + int_f *NB_p, /* Number of buttons (1..NB) */ + int_f *CHOICE_p, /* in: default button + out: selected button */ + int_f *IHELPS_p, + int_f *IHELPE_p, + char *HELPF_p, + int_f *IDEVH_p, + int_f *KBIN_p, + int_f *KBOUT_p, + int_fl TITLE_l, + int_fl PROMPT_l, + int_fl HELPF_l) +/* + * Present a ``radio button'' dialogue allowing the user to select + * one of a number of prompted items. + * In the text version this is simply a list of options and a prompt. + * CHOICE=(1..NB) => That option selected + * CHOICE=-1 => Cancel + */ +{ + char **opt, title[256]; + int i, numopts = (int)*NB_p; + + /* initialise options array */ + opt = (char **)malloc(numopts * sizeof(char *)); + opt[0] = (char *)malloc((unsigned int)((PROMPT_l+1) * numopts)); + for (i=0; i OK + * IOK=1 => Cancel + */ +{ + int ret; + char *prompts[4]; + int *bools[4]; + + prompts[0] = P1_p; bools[0] = (int *)C1_p; + prompts[1] = P2_p; bools[1] = (int *)C2_p; + prompts[2] = P3_p; bools[2] = (int *)C3_p; + prompts[3] = P4_p; bools[3] = (int *)C4_p; + + ret = checkn(4, prompts, bools); + + if (ret == 0) + *IOK_p = 0; + else + *IOK_p = 1; +} + +void getopt_x(int_f *KBIN_p, + int_f *KOPT_p, + int_f *IOPT_p) /* out: option number selected */ +/* + * Select a function to execute + * KOPT=0 => OK + * KOPT=1 => dialogue wanted + * KOPT=3 => menu option requested + */ +{ + *IOPT_p = (int_f)getcopt((int *)KOPT_p); + switch(*KOPT_p) { + case -3: + case -2: + *KOPT_p = 0; + *IOPT_p = 2; + break; + case -1: + *KOPT_p = 0; + *IOPT_p = 1; + break; + } +} + +void showfi_x(int_f *KBOUT_p, + char *STRING_p, + int_fl STRING_l) +/* + * Display `STRING', the name of the current file(s). + */ +{ + char func[256]; + + Fstr2Cstr(STRING_p, STRING_l, func, (int_fl)sizeof(func)); + showfi(func); +} + + +void showfu_x(int_f *KBOUT_p, + char *STRING_p, + int_fl STRING_l) +/* + * Display `STRING', the name of the current function. + */ +{ + char func[256]; + + Fstr2Cstr(STRING_p, STRING_l, func, (int_fl)sizeof(func)); + showfu(func); +} + +void errom_x(int_f *KBOUT_p, + char *STRING_p, + int_fl STRING_l) +/* + * Display the error message `STRING'. + */ +{ + char err[256]; + + Fstr2Cstr(STRING_p, STRING_l, err, (int_fl)sizeof(err)); + errom(err); +} + +void busy_x(int_f *KBOUT_p) +/* + * Inform the user that there will be a long pause before the + * next dialogue function is called. + */ +{ + busy(); +} + +/* + * Returns: + * 0 for ok + * -1 for cancel + */ +void bpause_x(int_f *KBIN_p, + int_f *KBOUT_p, + int_f *IOK_p) { + + *IOK_p = (int_f)bpause(); +} + diff --git a/src/staden/values.h b/src/staden/values.h new file mode 100644 index 0000000..792e966 --- /dev/null +++ b/src/staden/values.h @@ -0,0 +1,118 @@ +/* @(#)values.h 1.7 89/10/02 SMI; from S5R2 1.16 */ + +#ifndef _values_h +#define _values_h + +/* These values work with any binary representation of integers + * where the high-order bit contains the sign. */ + +/* a number used normally for size of a shift */ +#if gcos +#define BITSPERBYTE 9 +#else +#define BITSPERBYTE 8 +#endif +#define BITS(type) (BITSPERBYTE * (int)sizeof(type)) + +/* short, regular and long ints with only the high-order bit turned on */ +#define HIBITS ((short)(1 << BITS(short) - 1)) +#define HIBITI (1 << BITS(int) - 1) +#define HIBITL (1L << BITS(long) - 1) + +/* largest short, regular and long int */ +#define MAXSHORT ((short)~HIBITS) +#define MAXINT (~HIBITI) +#define MAXLONG (~HIBITL) + +/* various values that describe the binary floating-point representation + * _EXPBASE - the exponent base + * DMAXEXP - the maximum exponent of a double (as returned by frexp()) + * FMAXEXP - the maximum exponent of a float (as returned by frexp()) + * DMINEXP - the minimum exponent of a double (as returned by frexp()) + * FMINEXP - the minimum exponent of a float (as returned by frexp()) + * MAXDOUBLE - the largest double + ((_EXPBASE ** DMAXEXP) * (1 - (_EXPBASE ** -DSIGNIF))) + * MAXFLOAT - the largest float + ((_EXPBASE ** FMAXEXP) * (1 - (_EXPBASE ** -FSIGNIF))) + * MINDOUBLE - the smallest double (_EXPBASE ** (DMINEXP - 1)) + * MINFLOAT - the smallest float (_EXPBASE ** (FMINEXP - 1)) + * DSIGNIF - the number of significant bits in a double + * FSIGNIF - the number of significant bits in a float + * DMAXPOWTWO - the largest power of two exactly representable as a double + * FMAXPOWTWO - the largest power of two exactly representable as a float + * _IEEE - 1 if IEEE standard representation is used + * _DEXPLEN - the number of bits for the exponent of a double + * _FEXPLEN - the number of bits for the exponent of a float + * _HIDDENBIT - 1 if high-significance bit of mantissa is implicit + * LN_MAXDOUBLE - the natural log of the largest double -- log(MAXDOUBLE) + * LN_MINDOUBLE - the natural log of the smallest double -- log(MINDOUBLE) + * LN_MAXFLOAT - the natural log of the largest float + * LN_MINFLOAT - the natural log of the smallest float + */ +#if u3b || u3b5 || sun +#define MAXDOUBLE 1.797693134862315708e+308 +#define MAXFLOAT ((float)3.40282346638528860e+38) +#define MINDOUBLE 4.94065645841246544e-324 +#define MINFLOAT ((float)1.40129846432481707e-45) +#define _IEEE 1 +#define _DEXPLEN 11 +#define _HIDDENBIT 1 +#define DMINEXP (-(DMAXEXP + DSIGNIF - _HIDDENBIT - 3)) +#define FMINEXP (-(FMAXEXP + FSIGNIF - _HIDDENBIT - 3)) +#define LN_MAXFLOAT 8.8722839052068e+01 +#define LN_MINFLOAT -1.03278929903432e+02 +#endif +#if pdp11 || vax +#define MAXDOUBLE 1.701411834604692293e+38 +#define MAXFLOAT ((float)1.701411733192644299e+38) +/* The following is kludged because the PDP-11 compilers botch the simple form. + The kludge causes the constant to be computed at run-time on the PDP-11, + even though it is still "folded" at compile-time on the VAX. */ +#define MINDOUBLE (0.01 * 2.938735877055718770e-37) +#define MINFLOAT ((float)MINDOUBLE) +#define _IEEE 0 +#define _DEXPLEN 8 +#define _HIDDENBIT 1 +#define DMINEXP (-DMAXEXP) +#define FMINEXP (-FMAXEXP) +#endif +#if gcos +#define MAXDOUBLE 1.7014118346046923171e+38 +#define MAXFLOAT ((float)1.7014118219281863150e+38) +#define MINDOUBLE 2.9387358770557187699e-39 +#define MINFLOAT ((float)MINDOUBLE) +#define _IEEE 0 +#define _DEXPLEN 8 +#define _HIDDENBIT 0 +#define DMINEXP (-(DMAXEXP + 1)) +#define FMINEXP (-(FMAXEXP + 1)) +#endif +#if u370 +#define _LENBASE 4 +#else +#define _LENBASE 1 +#endif +#define _EXPBASE (1 << _LENBASE) +#define _FEXPLEN 8 +#define DSIGNIF (BITS(double) - _DEXPLEN + _HIDDENBIT - 1) +#define FSIGNIF (BITS(float) - _FEXPLEN + _HIDDENBIT - 1) +#define DMAXPOWTWO ((double)(1L << BITS(long) - 2) * \ + (1L << DSIGNIF - BITS(long) + 1)) +#define FMAXPOWTWO ((float)(1L << FSIGNIF - 1)) +#define DMAXEXP ((1 << _DEXPLEN - 1) - 1 + _IEEE) +#define FMAXEXP ((1 << _FEXPLEN - 1) - 1 + _IEEE) +#define LN_MAXDOUBLE (M_LN2 * DMAXEXP) +#define LN_MINDOUBLE (M_LN2 * (DMINEXP - 1)) + +#define H_PREC (DSIGNIF % 2 ? (1L << DSIGNIF/2) * M_SQRT2 : 1L << DSIGNIF/2) +#define X_EPS (1.0/H_PREC) +#define X_PLOSS ((double)(long)(M_PI * H_PREC)) +#define X_TLOSS (M_PI * DMAXPOWTWO) +#define M_LN2 0.69314718055994530942 +#define M_PI 3.14159265358979323846 +#define M_SQRT2 1.41421356237309504880 +#define MAXBEXP DMAXEXP /* for backward compatibility */ +#define MINBEXP DMINEXP /* for backward compatibility */ +#define MAXPOWTWO DMAXPOWTWO /* for backward compatibility */ + +#endif /*!_values_h*/ diff --git a/src/staden/vep.f b/src/staden/vep.f new file mode 100644 index 0000000..19c9259 --- /dev/null +++ b/src/staden/vep.f @@ -0,0 +1,771 @@ + SUBROUTINE FMAIN() + PARAMETER (MAXSEQ = 50000, + + MAXWLN = 6, + + LCONST = 4*MAXWLN, + + MAXWRD = 4**MAXWLN, + + MAXDEV = 5, + + NAMLEN = 60) + CHARACTER SEQV(MAXSEQ*2),SEQG(MAXSEQ),SEQC(MAXSEQ) + INTEGER WORDP(MAXWRD),POSN(MAXSEQ) + REAL HIST(-MAXSEQ:MAXSEQ) + INTEGER CONSTS(0:LCONST),DEVNOS(MAXDEV) + INTEGER SEQVI(MAXSEQ),SEQGI(MAXSEQ),RC + CHARACTER*(NAMLEN) FILNAM,HELPF,NAME,TNAME,VNAME + EXTERNAL LASTO +C +C 5-2-92 replaced parts of the output routines vpout, vpouts +C with routines fmtc and fmtg +C +C This routine prepares a reading for the assembly program. +C It compares the sequence against vectors and clips off any found +C Vector clipping is of 4 types: +C 1) find 5' cloning site +C 2) look for 3' cloning site +C 3) test for insert being all vector +C 4) look for cosmid "vector" +C The first 3 can all be performed using one sequence, and are made easier +C by telling the program exactly where the cloning site is, and which of +C the 3 it is performing. Types 1,2 and 3 look only in one orientation, +C whereas 4 should check both strands. Additionally they +C differ in their outcomes: 1 and 2 write a new file with the clippoints +C marked, 3 scrubs the reading (does not add it to a file of file names), +C 4 writes out a new file if the reading contains some non vector sequence. +C The clip should be marked differently for cosmid vector, just so we know where +C it is (it can be tagged). +C Clipping off crap should probably be done first by looking at the traces. +C What do we need to tell the program? +C 1) name of vector file +C 2) position of cloning site +C 3) position of primer +C It would be helpful to have a standard orientation for vector sequences +C for example Cloning site------ ... ------etis gninolC for ? strand +C +C producing the output files will be much simpler when we move to expt +C file format for the sequence and its annotation +C +C +C Initialise help - currently none +C CALL INTHLP('vep', TOPOPT) + ICG = 0 + ICB = 0 + IPG = 0 + IDM = 5 + CALL INITLU(IDM) + CALL UNITNO(KBIN,KBOUT,DEVNOS,MAXDEV) + IDEVNI = DEVNOS(1) + IDEVN = DEVNOS(5) + IDEVO = DEVNOS(2) + IDEVNG = DEVNOS(3) + IDEVNB = DEVNOS(4) + WRITE(KBOUT,*)'VEP v2.1: vector excising program' + FILNAM = ' ' + CALL OPENF1(DEVNOS(1),FILNAM,0,IOK,KBIN,KBOUT, + +'Input file of file names', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) STOP + FILNAM = 'm13mp18.seq' + CALL OPENF1(DEVNOS(2),FILNAM,0,IOK,KBIN,KBOUT, + +'File name of vector sequence', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) STOP +C +C simplify vector name to last field +C + VNAME = FILNAM(1+LASTO(FILNAM,'/'):) + IDIMV = MAXSEQ + CALL ARRFIL(DEVNOS(2),SEQV,IDIMV,KBOUT) + CLOSE(UNIT=DEVNOS(2)) +C check for contig header (should not be there) + IF(SEQV(20).EQ.'>') THEN + CALL SHFLCA(SEQV,MAXSEQ,21,1,IDIMV) + IDIMV = IDIMV - 20 + END IF + WRITE(KBOUT,*)'Vector length =',IDIMV +C cloning site used to distinguish search for cloning site +C from search for sequencing vector. A value of 0 signifies +C we are searching for cosmid vector + MN = 0 + MX = IDIMV + ICSITE = MIN(IDIMV,6249) + CALL GETINT(MN,MX,ICSITE, + +'3 prime side of cloning site (0 signifies cosmid vector)', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) STOP + ICSITE = IVAL + IPSITE = 1 + IF(ICSITE.NE.0) THEN + MN = -IDIMV + MX = IDIMV + IPSITE = 41 + CALL GETINT(MN,MX,IPSITE, + + 'Relative position of 3 prime end of primer site', + + IVAL,KBIN,KBOUT, + + IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) STOP + IPSITE = IVAL +C make cloning site end of seq, then start of seq is icsite + 1 + CALL SQCOPY(SEQV(1),SEQV(IDIMV+1),ICSITE) +C if forward primer then need to complement vector + IF(IPSITE.GT.0) THEN + CALL SQREV(SEQV(ICSITE+1),IDIMV) + CALL SQCOM(SEQV(ICSITE+1),IDIMV) + END IF + END IF + MN = 2 + MX = MAXWLN + LENGTH = 4 + CALL GETINT(MN,MX,LENGTH, + +'Word length', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) STOP + LENGTH = IVAL + MN = 1 + MX = 11 + LW = 7 + CALL GETINT(MN,MX,LW, + +'Number of diagonals to combine', + +IVAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) STOP + LW = IVAL + XMN = 0.1 + XMX = 1.0 + CUT = 0.35 + CALL GETRL(XMN,XMX,CUT, + +'Cutoff score', + +VAL,KBIN,KBOUT, + +IHELPS,IHELPE,HELPF,IDEVH,IOK) + IF(IOK.NE.0) STOP + CUT = VAL + FILNAM = ' ' + CALL OPENF1(DEVNOS(3),FILNAM,1,IOK,KBIN,KBOUT, + +'Output file of passed file names', + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) STOP + IF(ICSITE.EQ.0) THEN + FILNAM = ' ' + CALL OPENF1(DEVNOS(4),FILNAM,1,IOK,KBIN,KBOUT, + + 'Output file of failed file names', + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) STOP + END IF + IDE = (IDM-1)**LENGTH + CALL SETCN(CONSTS,LENGTH,IDM,LCONST) + CALL CONNUM(SEQV(ICSITE+1),SEQVI,IDIMV) + CALL ENCONC(SEQVI,IDIMV,POSN,WORDP,IDE,IDM,CONSTS,LENGTH, + +LCONST) + 10 CONTINUE + READ(IDEVNI,1002,END=100)NAME + CALL OPENRS(IDEVO,NAME,IOK,LRECL,2) + IF(IOK.NE.0) THEN + WRITE(KBOUT,*)'Error opening file',NAME + GO TO 10 + END IF + IDIMGI = MAXSEQ + CALL ARRFIM(IDEVO,SEQG,IDIMGI,KBOUT) + JGEL = JGEL + 1 + WRITE(KBOUT,*)'>>>> Read number',JGEL,' length',IDIMGI,' ',NAME +1002 FORMAT(A) +C LONG ENOUGH ? + IF(IDIMGI.LT.LENGTH)THEN + WRITE(KBOUT,*)' Gel reading too short to compare' + GO TO 10 + END IF + IDIMG = IDIMGI + IF(ICSITE.GT.0) THEN +C +C Sequencing vector clipping +C + CALL CONNUM(SEQG,SEQGI,IDIMG) + CALL VCUT(SEQVI,IDIMV,POSN,WORDP,IDE,SEQGI,IDIMG,CONSTS, + + LENGTH,IDM,LCONST,HIST,MAXSEQ,KBOUT,CUT,LW,LC,RC,ICSITE, + + IPSITE) +C WRITE(*,*)'LC,RC',LC,RC + CALL VPOUTS(IDEVO,NAME,LC,RC,SEQG,IDIMG,IDEVNG,IDEVN, + + VNAME,TNAME,ICB,ICG,IPG,IOK) + GO TO 10 + END IF +C +C Cosmid clipping +C + CALL CONNUM(SEQG,SEQGI,IDIMG) + CALL VCUT(SEQVI,IDIMV,POSN,WORDP,IDE,SEQGI,IDIMG,CONSTS, + + LENGTH,IDM,LCONST,HIST,MAXSEQ,KBOUT,CUT,LW,LC,RC,ICSITE, + + IPSITE) + IF(LC.NE.0) THEN +C +C Match found so do output +C + CALL VPOUT(IDEVO,NAME,LC,RC,SEQG,IDIMG,IDEVNG,IDEVNB,IDEVN, + + VNAME,TNAME,ICB,ICG,IPG,IOK) + GO TO 10 + ELSE +C +C Try other strand +C + CALL SQCOPY(SEQG,SEQC,IDIMG) + CALL SQREV(SEQC,IDIMG) + CALL SQCOM(SEQC,IDIMG) + CALL CONNUM(SEQC,SEQGI,IDIMG) + CALL VCUT(SEQVI,IDIMV,POSN,WORDP,IDE,SEQGI,IDIMG,CONSTS, + + LENGTH,IDM,LCONST,HIST,MAXSEQ,KBOUT,CUT,LW,LC,RC,ICSITE, + + IPSITE) + IF(LC.NE.0) THEN + LC1 = IDIMG - RC + 1 + RC = IDIMG - LC + 1 + LC = LC1 + END IF + CALL VPOUT(IDEVO,NAME,LC,RC,SEQG,IDIMG,IDEVNG,IDEVNB,IDEVN, + + VNAME,TNAME,ICB,ICG,IPG,IOK) + END IF + GO TO 10 + 100 CONTINUE + WRITE(KBOUT,*) + +'Finished after processing',JGEL,' files and finding' + WRITE(KBOUT,*)ICB,' completely vector' + WRITE(KBOUT,*)IPG,' partly vector' + WRITE(KBOUT,*)ICG,' free of vector' + END + INTEGER FUNCTION LASTO(STRING,C) + CHARACTER STRING*(*),C +C find last occurrence of C in STRING + J1 = 1 + J = 0 + L = LEN(STRING) + 10 CONTINUE + J1 = J1 + J + J = INDEX(STRING(J1:L),C) + IF(J.NE.0) GO TO 10 + LASTO = J1 - 1 + END + SUBROUTINE VPOUT(IDEVO,NAME,LC,RC,SEQ,IDIMG,IDEVNG,IDEVNB,IDEVN, + +VNAME,TNAME,ICB,ICG,IPG,IOK) + CHARACTER LINE*80,NAME*(*),MTYPE*4,SEQ(IDIMG),VNAME*(*),TNAME*(*) + CHARACTER*2 GOODS,LCLIPS,RCLIPS + PARAMETER (GOODS=' ',LCLIPS=';<',RCLIPS=';>') + INTEGER RC,FMTC + EXTERNAL NCFRS,FMTC +C +C IDEVO original file +C IDEVN new reading file +C IDEVNG fofn for good data +C IDEVNB fofn for bad data +C IDEVN = 6 +C WRITE(*,*)'LC,RC vpout',LC,RC +C +C Only handles one pair of clip points and assumes we discard lc to rc +C Possibilities and outcomes: +C 1. no vector: write name to fofn for good files +C 2. some vector: write name to fofn for good files, rename old file +C to oldname.vector name, rewrite sequence file with new +C clip points +C 3. all vector: write name to fofn for bad files +C +C +C If no vector: +C + IF(LC.EQ.0) THEN + CLOSE(IDEVO) + WRITE(IDEVNG,1005)NAME + ICG = ICG + 1 + RETURN + END IF +C +C If all vector: +C + IF(RC-LC+1.GE.IDIMG) THEN + CLOSE(IDEVO) + WRITE(IDEVNB,1005)NAME + 1005 FORMAT(A) + ICB = ICB + 1 + RETURN + END IF +C +C Some vector, so rename original file, and use old name for new file +C + TNAME = NAME + CLOSE(IDEVO) + K = INDEX(NAME,' ') + NAME(K:K) = '.' + NAME(K+1:) = VNAME + IOK = NCFRS(TNAME,NAME) + IF(IOK.NE.0) THEN + WRITE(*,*)'Error renaming',TNAME + RETURN + END IF +C +C Add the file name to the good file of file names +C + WRITE(IDEVNG,1005)TNAME + IPG = IPG + 1 +C +C Reopen the file with its new name +C + CALL OPENRS(IDEVO,NAME,IOK,LRECL,2) + IF(IOK.NE.0) THEN + WRITE(*,*)'Error opening file',NAME + RETURN + END IF +C +C Open the new file with the original name +C + CALL OPENRS(IDEVN,TNAME,IOK,LRECL,1) + IF(IOK.NE.0) THEN + WRITE(*,*)'Error opening file',NAME + RETURN + END IF + READ(IDEVO,1000,ERR=100)LOR,LCOR,LLOR,MTYPE,NAME + 1000 FORMAT(1X,3I6,A,A) + IF(LC.EQ.1) THEN + LCOR = LCOR + (RC - LC + 1) + LLOR = LLOR - (RC - LC + 1) + ELSE + LLOR = LC - 1 + END IF + WRITE(IDEVN,1006,ERR=100)LOR,LCOR,LLOR,MTYPE, + +NAME(1:INDEX(NAME,' ')-1) + 1006 FORMAT(';',3I6,A,A) + IF(FMTC(IDEVO,IDEVN,LINE,LCLIPS).NE.0) GO TO 100 +C +C now add left clip if required (lc=1) +C + IF(LC.EQ.1) THEN + K2 = 1 + CALL FMTV(SEQ,IDIMG,K2,RC,LINE,LCLIPS,IDEVN) +C +C now add in the right clip info +C + REWIND(IDEVO) + IF(FMTC(IDEVO,IDEVN,LINE,RCLIPS).NE.0) GO TO 100 +C +C Now do seq between RC and end as good seq +C + IF(RC.LT.IDIMG) THEN + K2 = RC + 1 + CALL FMTV(SEQ,IDIMG,K2,IDIMG,LINE,GOODS,IDEVN) + END IF + ELSE +C +C do seq in between LC and RC as a right clip (lc/=1) +C + K2 = LC + CALL FMTV(SEQ,IDIMG,K2,RC,LINE,RCLIPS,IDEVN) +C +C now add in the extant right clip info +C + REWIND(IDEVO) + IF(FMTC(IDEVO,IDEVN,LINE,RCLIPS).NE.0) GO TO 100 +C +C Do data up to lc as good seq +C + K2 = 1 + CALL FMTV(SEQ,IDIMG,K2,LC-1,LINE,GOODS,IDEVN) + END IF + CLOSE(IDEVO) + CLOSE(IDEVN) + IOK = 0 + RETURN + 100 CONTINUE + WRITE(*,*)'Error in VPOUT' + IOK = 1 + END + SUBROUTINE FMTV(SEQ,IDIMG,LC,RC,LINE,LT,IDEVN) + CHARACTER LINE*(*),SEQ(IDIMG),LT*(*) + INTEGER RC +C WRITE(*,*)'LC,RC IN FMTV',LC,RC +C +C write out seq from lc to rc with line type lt +C + K2 = LC - 1 + 10 CONTINUE + K1 = K2 + 1 + IF (K2.NE.RC) THEN + K2 = MIN(K1+49,RC) + WRITE(IDEVN,1000)LT,(SEQ(K),K=K1,K2) + GO TO 10 + END IF + 1000 FORMAT(A,50A1) + END + INTEGER FUNCTION FMTC(IDEVO,IDEVN,LINE,LT) + CHARACTER LINE*(*),LT*(*) + 10 CONTINUE + READ(IDEVO,1000,ERR=100,END=200)LINE + IF(LINE(1:2).EQ.LT) THEN + WRITE(IDEVN,1000,ERR=100)LINE + END IF + GO TO 10 + 100 CONTINUE + FMTC = 1 + RETURN + 200 CONTINUE + FMTC = 0 + 1000 FORMAT(A) + END + INTEGER FUNCTION NCFRS(FROM,TO) + CHARACTER FROM*(*),TO*(*) + INTEGER RENAME + EXTERNAL RENAME +C +C rename file using system call to RENAME +C + IFD = 0 + ITD = 0 + IF(INDEX(FROM,'/').EQ.0) THEN + FROM = './'//FROM + IFD = 1 + END IF + IF(INDEX(TO,'/').EQ.0) THEN + TO = './'//TO + ITD = 1 + END IF + NCFRS = RENAME(FROM,TO) + IF(IFD.EQ.1) FROM = FROM(3:) + IF(ITD.EQ.1) TO = TO(3:) + END + SUBROUTINE VPOUTS(IDEVO,NAME,LC,RC, + +SEQ,IDIMG,IDEVNG,IDEVN, + +VNAME,TNAME,ICB,ICG,IPG,IOK) + CHARACTER LINE*80,NAME*(*),MTYPE*4,SEQ(IDIMG),VNAME*(*),TNAME*(*) + CHARACTER*2 GOODS,LCLIPS,RCLIPS + PARAMETER (GOODS=' ',LCLIPS=';<',RCLIPS=';>') + INTEGER RC,FMTC + EXTERNAL NCFRS,FMTC +C +C IDEVO original file +C IDEVN new reading file +C IDEVNG fofn for good data +C IDEVN = 6 +C WRITE(*,*)'LC,RC',LC,RC +C +C handles 2 pairs of clip points +C Possibilities and outcomes: +C 1. no vector: write name to fofn for good files +C 2. some vector: write name to fofn for good files, rename old file +C to oldname.vector name, rewrite sequence file with new +C clip points +C 3. all vector: do nothing +C +C keep lc+1 to rc-1 ie discard 1 to lc and rc to end +C if no right cut we come in with rc = idimg+1 +C if no left cut lc = 0 +C +C If no vector: +C + IF((LC.EQ.0).AND.(RC.EQ.IDIMG+1)) THEN + CLOSE(IDEVO) + WRITE(IDEVNG,1005)NAME + ICG = ICG + 1 + RETURN + END IF +C +C If all vector: or < 20 bases left +C + IF(RC-LC.LT.20) THEN + CLOSE(IDEVO) + 1005 FORMAT(A) + ICB = ICB + 1 + RETURN + END IF +C +C Some vector, so rename original file, and use old name for new file +C + TNAME = NAME + CLOSE(IDEVO) + K = INDEX(NAME,' ') + NAME(K:K) = '.' + NAME(K+1:) = VNAME + IOK = NCFRS(TNAME,NAME) + IF(IOK.NE.0) THEN + WRITE(*,*)'Error renaming',TNAME + RETURN + END IF +C +C Add the file name to the good file of file names +C + WRITE(IDEVNG,1005)TNAME + IPG = IPG + 1 +C +C Reopen the file with its new name +C + CALL OPENRS(IDEVO,NAME,IOK,LRECL,2) + IF(IOK.NE.0) THEN + WRITE(*,*)'Error opening file',NAME + RETURN + END IF +C +C Open the new file with the original name +C + CALL OPENRS(IDEVN,TNAME,IOK,LRECL,1) + IF(IOK.NE.0) THEN + WRITE(*,*)'Error opening file',NAME + RETURN + END IF + READ(IDEVO,1000,ERR=5)LOR,LCOR,LLOR,MTYPE,NAME + 1000 FORMAT(1X,3I6,A,A) + GO TO 6 + 5 CONTINUE +C +C assume no header present (say scanner data) +C + LOR = IDIMG + LCOR = 0 + LLOR = IDIMG + MTYPE = 'PLN ' + NAME = ' ' + 6 CONTINUE + LCOR = LCOR + LC + LLOR = LLOR - (LC + IDIMG-RC+1) + WRITE(IDEVN,1006,ERR=100)LOR,LCOR,LLOR,MTYPE, + +NAME(1:MAX(1,INDEX(NAME,' ')-1)) + 1006 FORMAT(';',3I6,A,A) + IF(FMTC(IDEVO,IDEVN,LINE,LCLIPS).NE.0) GO TO 100 +C +C now add left clip if required (lc=1) +C + IF(LC.NE.0) THEN + K2 = 1 + CALL FMTV(SEQ,IDIMG,K2,LC,LINE,LCLIPS,IDEVN) + END IF +C +C now add new right clip if required +C + IF(RC.NE.IDIMG+1) THEN + K2 = RC + CALL FMTV(SEQ,IDIMG,K2,IDIMG,LINE,RCLIPS,IDEVN) + END IF +C +C now add in the extant right clip info +C + REWIND(IDEVO) + IF(FMTC(IDEVO,IDEVN,LINE,RCLIPS).NE.0) GO TO 100 +C +C Now do seq between LC+1 and RC-1 as good seq +C + K2 = LC + 1 + CALL FMTV(SEQ,IDIMG,K2,RC-1,LINE,GOODS,IDEVN) + CLOSE(IDEVO) + CLOSE(IDEVN) + IOK = 0 + RETURN + 100 CONTINUE + WRITE(*,*)'Error in VPOUTS' + IOK = 1 + END + SUBROUTINE VCUT(SEQV,IDIMV,POSN,WORDP,IDE,SEQH,IDIMH,CONSTS, + +LENGTH,IDM,LCONST,HIST,MAXSEQ,KBOUT,CUT,LW,LC,RC,ICSITE, + +IPSITE) + INTEGER SEQV(IDIMV),SEQH(IDIMH) + INTEGER POSN(IDIMV),WORDP(IDE),CONSTS(0:LCONST) + INTEGER RC + REAL HIST(-MAXSEQ:MAXSEQ) + EXTERNAL NCODEA +C CALL BUSY(KBOUT) + CALL FILLR(HIST(LENGTH-IDIMV),IDIMH+IDIMV+1,0.) + DO 20 I = 1,IDIMH-LENGTH+1 + J = NCODEA(SEQH(I),LENGTH,CONSTS,IDM,LCONST) + IF(J.NE.0)THEN + J1 = WORDP(J) + IF(J1.NE.0)THEN + K = I - J1 + HIST(K) = HIST(K) + 1. +10 CONTINUE + J2 = J1 + J1 = POSN(J2) + IF(J1.NE.0)THEN + K = I - J1 + HIST(K) = HIST(K) + 1. + GO TO 10 + END IF + END IF + END IF +20 CONTINUE + CALL PHIST(HIST,IDIMV,IDIMH,LENGTH,MAXSEQ) + IF(ICSITE.NE.0) THEN +C +C look for primer region +C + CALL FCUT(HIST,IDIMV,IDIMH,LENGTH, + + MAXSEQ,CUT,LW,LC,RC,IPSITE,1) +C WRITE(*,*)'LC',LC +C WRITE(*,*)'RC',RC + IF (LC.EQ.0) THEN +C WRITE(KBOUT,*)' ***** No primer site found ********' + ELSE + LC = RC + END IF +C +C look for running into vector at cloning site +C + CALL FCUT(HIST,IDIMV,IDIMH,LENGTH, + + MAXSEQ,CUT,LW,LCR,IRCR,IPSITE,2) +C +C set right cut to 1 past the end of the sequence +C +C WRITE(*,*)'LCR',LCR + RC = IDIMH + 1 + IF(LCR.GT.0) THEN + RC = LCR + END IF + ELSE +C +C look for cosmid vector +C + CALL FCUT(HIST,IDIMV,IDIMH,LENGTH, + + MAXSEQ,CUT,LW,LC,RC,IPSITE,3) + IF(LC.GT.0) THEN +C WRITE(KBOUT,*) +C +'>>>>>>>>>>>>>>>>>>>>>>>>diagonal found' + RETURN + END IF + END IF + END + SUBROUTINE PHIST(HIST,IDIMV,IDIMH,LENGTH,MAXSEQ) + REAL HIST(-MAXSEQ:MAXSEQ) + IF(IDIMV.GE.IDIMH) THEN + D = LENGTH + DO 10 I=LENGTH-IDIMV,IDIMH-IDIMV-1 + HIST(I) = HIST(I)/D + D = D + 1 + 10 CONTINUE + D = IDIMH + DO 20 I=IDIMH-IDIMV,0 + HIST(I) = HIST(I)/D + 20 CONTINUE + D = IDIMH - 1 + DO 30 I=1,IDIMH-LENGTH + HIST(I) = HIST(I)/D + D = D - 1 + 30 CONTINUE + ELSE + D = LENGTH + DO 40 I=LENGTH-IDIMV,-1 + HIST(I) = HIST(I)/D + D = D + 1 + 40 CONTINUE + D = IDIMV + DO 50 I=0,IDIMH-IDIMV + HIST(I) = HIST(I)/D + 50 CONTINUE + D = IDIMV - 1 + DO 60 I=IDIMH-IDIMV+1,IDIMH-LENGTH + HIST(I) = HIST(I)/D + D = D - 1 + 60 CONTINUE + END IF + END + SUBROUTINE FCUT(HIST,IDIMV,IDIMH,LENGTH, + + MAXSEQ,CUT,LW,LC,RC,PSITE,JOB) + REAL HIST(-MAXSEQ:MAXSEQ) + INTEGER RC,PSITE +C PSITE is primer site + LC = 0 + RC = 0 + DMAX = 0. +C If job = 1 look for cloning site from psite to the end of the vector +C if job = 2 look for vector in the rest of the sequence +C If job = 3 look for cosmid vector in whole of sequence +C We discard lc to rc inclusive, lc=0 means discard nothing + IF(JOB.EQ.1) THEN + I1 = LENGTH - IDIMV + I2 = -ABS(PSITE) + ELSE IF(JOB.EQ.2) THEN + I1 = -ABS(PSITE) + I2 = IDIMH - LENGTH + ELSE IF(JOB.EQ.3) THEN + I1 = LENGTH - IDIMV + I2 = IDIMH - LENGTH + ELSE + WRITE(*,*)'Error in FCUT' + RETURN + END IF +C WRITE(*,*)'LOOKING AT ',I1,I2 + DO 10 I=I1,I2 + DT = HIST(I) + IF(DT.GT.DMAX) THEN + DMAX = DT + ID = I + END IF + 10 CONTINUE + D = 0. + DO 35 I=MAX(ID-LW/2,LENGTH-IDIMV), + + MIN(ID+LW/2,IDIMH-LENGTH) + D = D + HIST(I) + 35 CONTINUE +C WRITE(*,*)'Best diagonal, score and local sum',ID,DMAX,D +C WRITE(*,1000)ID,DMAX,D + 1000 FORMAT(I6,2F10.3) + IF(D.LT.CUT) RETURN + IF(IDIMV.GE.IDIMH) THEN + IF(ID.GE.0) THEN + LC = ID + 1 + RC = IDIMH + ELSE + LC = 1 + RC = MIN(IDIMH,IDIMV + ID) + END IF + ELSE + IF(ID.GE.0) THEN + LC = ID + 1 + RC = MIN(ID+IDIMV,IDIMH) + ELSE + LC = 1 + RC = ID + IDIMV + END IF + END IF + WRITE(*,*)' Discard ',LC, ' to ',RC + END + SUBROUTINE ARRFIM(IDEV,SEQNCE,J,KBOUT) +C 14-8-91 Added err= option to read, and set length to 0 if error found +C AUTHOR: RODGER STADEN + CHARACTER TEMP(80),SEQNCE(J) + CHARACTER SPACE,ENDCHR,TITCHR + SAVE ENDCHR,SPACE,TITCHR + DATA ENDCHR/'@'/ + DATA SPACE/' '/ + DATA TITCHR/';'/ + IDMX=J + J=0 +1 CONTINUE + READ(IDEV,1001,END=30,ERR=40)TEMP +1001 FORMAT(80A1) + IF(TEMP(1).EQ.TITCHR)THEN +C WRITE(KBOUT,1003)(TEMP(K),K=2,80) +C1003 FORMAT(' ',79A1) + GO TO 1 + END IF +10 CONTINUE + DO 20 I=1,80 + IF(TEMP(I).NE.SPACE)THEN + IF(TEMP(I).EQ.ENDCHR)RETURN + IF(J.EQ.IDMX)THEN + WRITE(KBOUT,1002)IDMX +1002 FORMAT( + + ' Too much data. Maximum possible', + + ' =',I6,', input stopped there') + RETURN + END IF + J=J+1 + SEQNCE(J)=TEMP(I) + END IF +20 CONTINUE + GO TO 1 +30 CONTINUE + RETURN + 40 CONTINUE + CALL ERROM(KBOUT,'Error reading file') + J = 0 + END + SUBROUTINE SHFLCA(STRING,MAXAR,FROMS,TO,FROME) + CHARACTER STRING(MAXAR) + INTEGER FROMS,FROME,TO +C +C shift left from from to to +C + J = TO + DO 10 I=FROMS,FROME + STRING(J) = STRING(I) + J = J + 1 + 10 CONTINUE + END diff --git a/src/staden/xdapSpec.c b/src/staden/xdapSpec.c new file mode 100644 index 0000000..cb71d49 --- /dev/null +++ b/src/staden/xdapSpec.c @@ -0,0 +1,432 @@ +/* + Title: xdapSpec + + File: xdapSpec.c + Purpose: Definitions specific to the `xdap' program + Last update: Fri Dec 14 1990 + + Change log: + + 3/11/90 SD Added extra menu option + 22 August 1991 SD Added new menu option (Find internal joins) + and associated help structures +*/ + + +/* + This module contains data specific to the menus and help system + for the `xdap' program. +*/ + + + + +/* ---- Includes ---- */ + +#include "fort.h" +#include "progSpec.h" +#include "userface.h" /* IMPORT: menu_x */ + +#include +#include +#include +#include +#include +#include +#include + + + + +/* ---- Types ---- */ + + +typedef struct +{ String name; /* Name of function */ + int number; /* Number of function */ +} MenuData, *MenuDataList; + + + + +/* ---- Static variables ---- */ + + +static Widget menubarWid = (Widget)NULL; /* Initialised by CreateProgMenus */ + + +static MenuData general_menu_data[] = +{ {"Open a database", 3}, + {"Display a contig", 5}, + {"List a text file", 6}, + {"Redirect output", 7}, + {"Calculate a consensus", 8}, + {"Screen against restriction enzymes", 17}, + {"Screen against vector", 18}, + {"Check database", 19}, + {"Copy database", 24}, + {"Show relationships", 25}, + {"Set parameters", 27}, + {"Highlight disagreements", 28}, + {"Examine quality", 29}, + {"Find internal joins", 35}, +}; + + +static MenuData screen_menu_data[] = +{ {"Clear graphics", 10}, + {"Clear text", 11}, + {"Draw ruler", 12}, + {"Use cross hair", 13}, + {"Change margins", 14}, + {"Label diagram", 15}, + {"Plot map", 16}, + {"Plot single contig", 33}, + {"Plot all contigs", 34}, +}; + + +static MenuData modification_menu_data[] = +{ {"Edit contig", 4}, + {"Auto assemble", 20}, + {"Join contigs", 22}, + {"Complement a contig", 23}, + {"Alter relationships", 26}, + {"Extract gel readings", 32}, +}; + + +static MenuData enter_menu_data[] = +{ {"Cancel", 2}, + {"Complete entry", 3}, + {"Edit contig", 4}, + {"Display", 5}, + {"Edit new gel reading", 6}, +}; + + +static MenuData join_menu_data[] = +{ {"Cancel", 2}, + {"Complete join", 3}, + {"Edit left contig", 4}, + {"Display join", 5}, + {"Edit right contig", 6}, + {"Move join", 7}, +}; + + +static MenuData alter_menu_data[] = +{ {"Cancel", 2}, + {"Line change", 3}, + {"Edit gel reading", 4}, + {"Delete contig", 5}, + {"Shift", 6}, + {"Move gel reading", 7}, + {"Rename gel reading", 8}, + {"Break a contig", 9}, + {"Remove a gel reading", 10}, + {"Alter raw data parameters", 11}, +}; + + +static MenuData edit_menu_data[] = +{ {"Cancel", 2}, + {"Insert", 3}, + {"Delete", 4}, + {"Change", 5}, +}; + + + + +/* --- Callback functions ---- */ + +static XtCallbackProc externalCallbackProc; +static XtPointer externalClient_data; + +static void MenuSelectCallback(Widget w, XtPointer i, XtPointer junk) +/* + Pass the menu item callback back to `externalCallbackProc' + which the user supplied to `CreateProgMenus'. +*/ +{ externalCallbackProc(w, externalClient_data, i); +} + + + + +/* ---- Private functions ---- */ + + +static void CreateMenu(Widget parentWid, String menuButtonName, + MenuDataList md, Cardinal num_md) +{ Widget buttonWid, menuWid; + int i; + + /* + Create the (empty) menu button. + The button is initially unmanaged within its parent. + */ + buttonWid = XtCreateWidget(menuButtonName, menuButtonWidgetClass, + parentWid, NULL, 0); + menuWid = XtCreatePopupShell("menu", simpleMenuWidgetClass, buttonWid, + NULL, 0); + + /* + Put the individual items in. + When selected, each entry will generate a callback with + its associated number. + */ + for (i = 0; i < (int) num_md ; i++) + { Widget entryWid = XtCreateManagedWidget(md[i].name, smeBSBObjectClass, + menuWid, NULL, 0); + XtAddCallback(entryWid, XtNcallback, MenuSelectCallback, + (XtPointer) md[i].number); + } + +} + + + + +/* ---- Exported functions ---- */ + + +void CreateProgMenus(Widget parentWid, + XtCallbackProc cbp, XtPointer client_data) +/* + Install the menus for this program into `parentWid'. + They are initially all unmanaged. + When pressed, each item (which is the name of a function) will + call `cbp' providing `client_data' and the number of the function + as `call_data'. +*/ +{ externalCallbackProc = cbp; + externalClient_data = client_data; + menubarWid = parentWid; + + CreateMenu(parentWid, "General", + general_menu_data, XtNumber(general_menu_data)); + CreateMenu(parentWid, "Screen", + screen_menu_data, XtNumber(screen_menu_data)); + CreateMenu(parentWid, "Modification", + modification_menu_data, XtNumber(modification_menu_data)); + CreateMenu(parentWid, "Enter", + enter_menu_data, XtNumber(enter_menu_data)); + CreateMenu(parentWid, "Join", + join_menu_data, XtNumber(join_menu_data)); + CreateMenu(parentWid, "Alter", + alter_menu_data, XtNumber(alter_menu_data)); + CreateMenu(parentWid, "Edit", + edit_menu_data, XtNumber(edit_menu_data)); +} + + + + +/* + Special menu functions for SAP. + + The generic menu functions, found in 'dialogues' are only + used indirectly by SAP. + + SUBROUTINE DBMENT(MENU,NOPT,MAXOPT, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + CHARACTER HELPF*(*) + INTEGER IHELPS(0:MAXOPT),IHELPE(0:MAXOPT) + + SUBROUTINE DBMENU(MENU,NOPT,IHELPS,IHELPE,HELPF,IDEVH, + +KBIN,KBOUT) + CHARACTER HELPF*(*) + INTEGER IHELPS,IHELPE + + If this code were in 'dialogues', then we wouldn't need to + keep the reference to 'menubarWid' which is making a big + assumption anyway. +*/ + +#define dbment_x dbment_ +#define dbmenu_x dbmenu_ + + +void dbment_x(int_f *MENU_p, + int_f *NOPT_p, + int_f *MAXOPT_p, + int_f *IHELPS_p, + int_f *IHELPE_p, + char *HELPF_p, + int_f *IDEVH_p, + int_f *KBIN_p, + int_f *KBOUT_p, + int_fl HELPF_l) +{ WidgetList sprogs; + int nSprogs; + int_f KOPT, MOPT, MINMEM; /* Dummy arguments to keep menu_x happy */ + Arg args[10]; + int nargs; + + + /* + All the menus are children of 'menubarWid'. + Manage just the 'top level' menus. + */ + nargs = 0; + XtSetArg(args[nargs], XtNchildren, &sprogs); nargs++; + XtSetArg(args[nargs], XtNnumChildren, &nSprogs); nargs++; + XtGetValues(menubarWid, args, nargs); + XtUnmanageChildren(sprogs, nSprogs); + + XtManageChild(XtNameToWidget(menubarWid, "General")); + XtManageChild(XtNameToWidget(menubarWid, "Screen")); + XtManageChild(XtNameToWidget(menubarWid, "Modification")); + + + /* + Run the menu with the usual mechanism. + */ + menu_x( NOPT_p, + &KOPT, + &MOPT, + MAXOPT_p, + &MINMEM, + KBIN_p, + KBOUT_p, + IHELPS_p, + IHELPE_p, + HELPF_p, + IDEVH_p, + HELPF_l); +} + + +void dbmenu_x(int_f *MENU_p, + int_f *NOPT_p, + int_f *IHELPS_p, + int_f *IHELPE_p, + char *HELPF_p, + int_f *IDEVH_p, + int_f *KBIN_p, + int_f *KBOUT_p, + int_fl HELPF_l) +{ WidgetList sprogs; + int nSprogs; + int_f KOPT, MOPT, MINMEM, MAXOPT; /* Dummy arguments to keep menu_x happy */ + Arg args[10]; + int nargs; + + + /* + All the menus are children of 'menubarWid'. + Manage just the menu specified by 'MENU'. + */ + nargs = 0; + XtSetArg(args[nargs], XtNchildren, &sprogs); nargs++; + XtSetArg(args[nargs], XtNnumChildren, &nSprogs); nargs++; + XtGetValues(menubarWid, args, nargs); + XtUnmanageChildren(sprogs, nSprogs); + + switch (*MENU_p) + { case 2: XtManageChild(XtNameToWidget(menubarWid, "Enter")); + break; + + case 3: XtManageChild(XtNameToWidget(menubarWid, "Join")); + break; + + case 4: XtManageChild(XtNameToWidget(menubarWid, "Alter")); + break; + + case 5: XtManageChild(XtNameToWidget(menubarWid, "Edit")); + break; + } + + /* + Run the menu with the usual mechanism. + */ + menu_x( NOPT_p, + &KOPT, + &MOPT, + &MAXOPT, + &MINMEM, + KBIN_p, + KBOUT_p, + IHELPS_p, + IHELPE_p, + HELPF_p, + IDEVH_p, + HELPF_l); + +} + + + + + +const int botHelpOpt = 0; +const int topHelpOpt = 35; +/* + The range of option numbers for the help system. +*/ + + +const char helpTextFN[] = "DAPHELP"; +const char helpPtrsFN[] = "DAPHPNT"; +/* + File names for the help text and pointer files. +*/ + + +const char *helpTopics[] = +{ "SAP", + "Help", + "Quit", + "Open a database", + "Edit contig", + "Display a contig", + "List a text file", + "Direct output to disk", + "Calculate a consensus", + "Screen edit", + "Clear graphics", + "Clear text", + "Draw ruler", + "Use cross hair", + "Change margins", + "Plot map", + "Label diagram", + "Screen against restriction enzymes", + "Screen against vector", + "Check consistency", + "Auto assemble", + "Enter new gel reading", + "Join contigs", + "Complement a contig", + "Copy database", + "Show relationships", + "Alter relationships", + "set parameters", + "Highlight disagreements", + "Examine quality", + "Auto edit a contig", + "Type in gel readings", + "Extract gel readings", + "Plot single contig", + "Plot all contigs", + "Find internal joins", + NULL, +}; +/* + Help topics, indexed in C between 0 and topHelpOpt-botHelpOpt + but referring to topics botHelpOpt to topHelpOpt +*/ + + + + + + + + + + + + diff --git a/src/staden/xdapmain.c b/src/staden/xdapmain.c new file mode 100644 index 0000000..cbd0def --- /dev/null +++ b/src/staden/xdapmain.c @@ -0,0 +1,549 @@ +/* + Title: main + + File: main.c + Purpose: C language entry point and initialisation functions + Last update: Monday 20 January 1992 +*/ + + +/* + This module contains the C language entry point `main' and + initialisation for the X system. It provides the main event loop, + as far as X is concerned. + + Three top-level shells are created: control, dialogue and help. +*/ + + + + +/* ---- Includes ---- */ + +#include /* IMPORT: freopen, stdout, stderr, fprintf */ +#include "mystdlib.h" /* IMPORT: exit */ + +#include "main.h" +#include "dialogues.h" /* IMPORT: CreateDialogueShell */ +#include "help.h" /* IMPORT: CreateHelpShell */ +#include "plX.h" /* IMPORT: CreateGraphicsOutput */ +#include "textOutput.h" /* IMPORT: CreateTextOutput + UpdateTextOutput */ +#include "contigEditor.h" /* IMPORT: CreateEditorShell */ +#include "locks.h" +#include "mcspec.h" +#include "postscript.h" /* IMPORT: closep_x() */ + +#include +#include +#include +#include + + + + +/* ---- Types ---- */ +typedef enum {Alive, Dying, Dead} LifeForce; + + + + +/* ---- Static variables ---- */ + +static unsigned int global_argc; +static char **global_argv; +static XtAppContext app_con; +static Display *display; +static Widget toplevelWid; +static Widget dialogueShellWid, helpShellWid, outputShellWid,graphicsShellWid; +static LifeForce lifeForce = Alive; +static int exitcode; +/* +static XrmOptionDescRec options[] = +{ +}; +*/ +/* The following are used if Xdap cannot be found */ +static String fallback_resources[] = { + /* Dialogue shell */ + /* -------------- */ + "Xdap.Dialogue.allowShellResize: False", + "Xdap.Dialogue.width: 550", + "Xdap.Dialogue.minWidth: 550", + "Xdap.Dialogue.maxWidth: 550", + "Xdap.Dialogue.height: 180", + "Xdap.Dialogue.minHeight: 180", + "Xdap.Dialogue.maxHeight: 180", + "Xdap.Dialogue.dialogueForm*translations: #override \\n\ + Return: CRAction()", + /* The default chaining for all children is Top,Top,Left,Left */ + "Xdap.Dialogue.dialogueForm*top: chainTop", + "Xdap.Dialogue.dialogueForm*bottom: chainTop", + "Xdap.Dialogue.dialogueForm*left: chainLeft", + "Xdap.Dialogue.dialogueForm*right: chainLeft", + /* currFile */ + "Xdap.Dialogue.dialogueForm.currFile.label: Current file:", + "Xdap.Dialogue.dialogueForm.currFile.borderWidth: 0", + "Xdap.Dialogue.dialogueForm.currFile.resizable: True", + /* currFunc */ + "Xdap.Dialogue.dialogueForm.currFunc.label: Current function:", + "Xdap.Dialogue.dialogueForm.currFunc.borderWidth: 0", + "Xdap.Dialogue.dialogueForm.currFunc.resizable: True", + /* quit */ + "Xdap.Dialogue.dialogueForm.quit.label: Quit application", + "Xdap.Dialogue.dialogueForm.quit.foreground: red", + "Xdap.Dialogue.dialogueForm.quit.borderColor: red", + + /* null */ + "Xdap.Dialogue.dialogueForm.null.borderWidth: 0", + "Xdap.Dialogue.dialogueForm.null.label: Working ... ", + + /* nChoice */ + "Xdap.Dialogue.dialogueForm.nChoice.resizable: True", + /* nChoice buttons */ + "Xdap.Dialogue.dialogueForm.nChoice.buttons.resizable: True", + /* nChoice OK */ + "Xdap.Dialogue.dialogueForm.nChoice.OK.borderWidth: 3", + + /* getopt */ + "Xdap.Dialogue.dialogueForm.getopt.width: 540", + /* getopt execlabel */ + "Xdap.Dialogue.dialogueForm.getopt.execlabel.label: Select a function to:", + "Xdap.Dialogue.dialogueForm.getopt.execlabel.borderWidth: 0", + /* getopt execcommand */ + "Xdap.Dialogue.dialogueForm.getopt.execcommand.label: Execute with dialogue", + "Xdap.Dialogue.dialogueForm.getopt.execcommand.resizable: True", + /* getopt menubar */ + "Xdap.Dialogue.dialogueForm.getopt.menubar.width: 530", + "Xdap.Dialogue.dialogueForm.getopt.menubar.height: 25", + + /* getstring */ + "Xdap.Dialogue.dialogueForm.getstring.label:", + "Xdap.Dialogue.dialogueForm.getstring.value:", + "Xdap.Dialogue.dialogueForm.getstring.resizable: True", + /* getstring label */ + "Xdap.Dialogue.dialogueForm.getstring.label.resizable: True", + /* getstring value */ + "Xdap.Dialogue.dialogueForm.getstring.value.width: 350", + /* getstring OK */ + "Xdap.Dialogue.dialogueForm.getstring.OK.borderWidth: 3", + + /* yesno */ + "Xdap.Dialogue.dialogueForm.yesno.resizable: True", + /* yesno label */ + "Xdap.Dialogue.dialogueForm.yesno.lab.label:", + "Xdap.Dialogue.dialogueForm.yesno.lab.borderWidth: 0", + "Xdap.Dialogue.dialogueForm.yesno.lab.resizable: True", + "Xdap.Dialogue.dialogueForm.yesno.YES.borderWidth: 3", + + /* Help shell */ + /* ---------- */ + "Xdap.Help.width: 550", + "Xdap.Help.minWidth: 550", + "Xdap.Help.height: 400", + "Xdap.Help.minHeight: 150", + "Xdap.Help*top: chainTop", + "Xdap.Help*bottom: chainTop", + "Xdap.Help*left: chainLeft", + "Xdap.Help*right: chainLeft", + + /* currDisp */ + "Xdap.Help.helpForm.currDisp.resizable: True", + "Xdap.Help.helpForm.currDisp.borderWidth: 0", + /* rmHelp */ + "Xdap.Help.helpForm.rmHelp.label: Remove help", + /* button */ + "Xdap.Help.helpForm.button.label: List of topics", + /* topicsViewport */ + "Xdap.Help.helpForm.topicsViewport.allowVert: True", + "Xdap.Help.helpForm.topicsViewport.bottom: chainBottom", + "Xdap.Help.helpForm.topicsViewport.right: chainRight", + "Xdap.Help.helpForm.topicsViewport.topicsList.defaultColumns: 1", + "Xdap.Help.helpForm.topicsViewporttopicsList.forceColumns: 2", + /* text */ + "Xdap.Help.helpForm.text.editType: edit", + "Xdap.Help.helpForm.text.scrollVertical: always", + "Xdap.Help.helpForm.text.bottom: chainBottom", + "Xdap.Help.helpForm.text.right: chainRight", + + /* Output shell */ + /* ------------ */ + "Xdap.Graphics.graph.width: 550", + "Xdap.Graphics.graph.height: 400", + "Xdap.Output.text.width: 550", + "Xdap.Output.text.height: 100", + + /* Editor shell */ + /* ------------ */ + "Xdap.Editor.allowShellResize: True", + "Xdap.Editor.mainForm.resizable: True", +/* + "Xdap.Editor*font: 5x8", +*/ + "Xdap.Editor*font: *Fixed-*-20-200-*", + /* The default chaining for all children is Top,Top,Left,Left */ + "Xdap.Editor.mainForm*top: chainTop", + "Xdap.Editor.mainForm*bottom: chainTop", + "Xdap.Editor.mainForm*left: chainLeft", + "Xdap.Editor.mainForm*right: chainLeft", + "Xdap.Editor.mainForm.title.borderWidth: 0", + "Xdap.Editor.mainForm.title.label: Contig Editor (V1.1)", + "Xdap.Editor.mainForm.buttons.borderWidth: 0", + "Xdap.Editor.mainForm.buttons.orientation: horizontal", + "Xdap.Editor.mainForm.buttons.resizable: True", + "Xdap.Editor.mainForm.buttons*vertDistance: 0", + "Xdap.Editor.mainForm.buttons.insert.label: Insert", + "Xdap.Editor.mainForm.buttons.replace.label: Replace", + "Xdap.Editor.mainForm.buttons.replace.state: True", + "Xdap.Editor.mainForm.buttons.superman.label: Super Edit", + "Xdap.Editor.mainForm.buttons.superman.state: False", + "Xdap.Editor.mainForm.buttons.reveal.label: Reveal Cutoffs", + "Xdap.Editor.mainForm.buttons.reveal.state: False", + "Xdap.Editor.mainForm.buttons.undo.label: Undo", + "Xdap.Editor.mainForm.buttons.save.label: Save", + "Xdap.Editor.mainForm.buttons.exit.label: Leave Editor", + "Xdap.Editor.mainForm.buttons.exit.foreground: red", + "Xdap.Editor.mainForm.buttons.exit.borderColor: red", + "Xdap.Editor.mainForm.buttons.lock.label: Lock", + "Xdap.Editor.mainForm.buttons.join.label: Join", + "Xdap.Editor.mainForm.buttons.next.label: Next Problem", + "Xdap.Editor.mainForm.edForm.resizable: True", + "Xdap.Editor.mainForm.edForm.defaultDistance: 0", + "Xdap.Editor.mainForm.edForm.borderWidth: 0", + "Xdap.Editor.mainForm.edForm.sequences.resizable: True", + "Xdap.Editor.mainForm.edForm.names.resizable: True", + "Xdap.Editor.mainForm.edForm.slider.length: 500", + "Xdap.Editor.mainForm.edForm.slider.orientation: horizontal", + "Xdap.Editor.mainForm.edForm.scrollButtons.defaultDistance: 0", + "Xdap.Editor.mainForm.edForm.scrollButtons.borderWidth: 0", + "Xdap.Editor.mainForm.edForm.scrollButtons.left: ChainLeft", + "Xdap.Editor.mainForm.edForm.scrollButtons.right: ChainLeft", + "Xdap.Editor.mainForm.edForm.scrollButtons.*.resizable: True", + "Xdap.Editor.mainForm.edForm.scrollButtons.*.resize: True", + "Xdap.Editor.mainForm.edForm.scrollButtons.*.left: Rubber", + "Xdap.Editor.mainForm.edForm.scrollButtons.*.right: Rubber", + "Xdap.Editor.mainForm.edForm.scrollButtons.leftleft.label: <<", + "Xdap.Editor.mainForm.edForm.scrollButtons.left.label: <", + "Xdap.Editor.mainForm.edForm.scrollButtons.right.label: >", + "Xdap.Editor.mainForm.edForm.scrollButtons.rightright.label: >>", + "Xdap.Editor*warnPrompt.label: Save changes? ", + "Xdap.Editor*warnBox.borderWidth: 0", + "Xdap.Editor*warnBox.orientation: vertical", + "Xdap.Editor*warnPrompt.borderWidth: 0", + "Xdap.Editor.mainForm.disagreeForm.resizable: True", + "Xdap.Editor.mainForm.disagreeForm.dummy.resizable: True", + "Xdap.Editor.mainForm.disagreeForm.disagreements.resizable: True", + "Xdap.Editor.mainForm.disagreeForm.borderWidth: 0", + "Xdap.Editor.mainForm.disagreeForm.defaultDistance: 0", + + /* Trace Manager */ + /* ------------- */ + "*Traces.mainForm.buttons.borderWidth: 0", + "*Traces.mainForm.buttons.orientation: horizontal", + "*Traces.mainForm.buttons*top: ChainTop", + "*Traces.mainForm.buttons*bottom: ChainTop", + "*Traces.mainForm.buttons*left: ChainLeft", + "*Traces.mainForm.buttons*right: ChainLeft", + + "*Traces.allowShellResize: True", + "*Traces.mainForm.traceForm.resizable: True", + "*Traces.mainForm.traceForm.form.resizable: True", + "*Traces.mainForm.defaultDistance: 0", + "*Traces.mainForm.traceForm.form.defaultDistance: 1", + "*Traces.mainForm.traceForm.borderWidth: 0", + "*Traces.mainForm.traceForm.form.borderWidth: 0", + + /* The main display: a viewport containing a form containing graphs */ + "*Traces.mainForm.traceForm.form.top: Rubber", + "*Traces.mainForm.traceForm.form.bottom: Rubber", + "*Traces.mainForm.traceForm.form.left: ChainLeft", + "*Traces.mainForm.traceForm.form.right: ChainRight", + "*Traces.mainForm.traceForm.form.viewport.top: Rubber", + "*Traces.mainForm.traceForm.form.viewport.bottom: Rubber", + "*Traces.mainForm.traceForm.form.viewport.left: ChainLeft", + "*Traces.mainForm.traceForm.form.viewport.right: ChainRight", + "*Traces.mainForm.traceForm.form.viewport.allowHoriz: True", + "*Traces.mainForm.traceForm.form.viewport.forcebars: True", + "*Traces.mainForm.traceForm.form.viewport.width: 700", + "*Traces.mainForm.traceForm.form.viewport.height: 150", + "*Traces.mainForm.traceForm.form.viewport.vpForm.defaultDistance: 0", + "*Traces.mainForm.traceForm.form.viewport.vpForm.Graph.resizable: True", + "*Traces.mainForm.traceForm.form.viewport.vpForm.Graph.top: ChainTop", + "*Traces.mainForm.traceForm.form.viewport.vpForm.Graph.bottom: ChainTop", + "*Traces.mainForm.traceForm.form.viewport.vpForm.Graph.left: ChainLeft", + "*Traces.mainForm.traceForm.form.viewport.vpForm.Graph.right: ChainRight", + "*Traces.mainForm.traceForm.form.viewport.vpForm.trace.top: ChainTop", + "*Traces.mainForm.traceForm.form.viewport.vpForm.trace.bottom: ChainBottom", + "*Traces.mainForm.traceForm.form.viewport.vpForm.Graph.font: -misc-*-bold-*15-*", + /* The text graph heights are dynamically set to accomodate the font */ + /* The graphs are dynamically initially set to fill the viewport */ + "*Traces.mainForm.traceForm.form.viewport.vpForm.Graph.dimBackground: grey", + "*Traces.mainForm.traceForm.form.viewport.vpForm.Graph.graphColour1: green3", + "*Traces.mainForm.traceForm.form.viewport.vpForm.Graph.graphColour2: purple", + "*Traces.mainForm.traceForm.form.viewport.vpForm.Graph.graphColour3: black", + "*Traces.mainForm.traceForm.form.viewport.vpForm.Graph.graphColour4: red", + + + /* Gadgets */ + "*Traces.mainForm.traceForm.form.gadgets.borderWidth: 0", + "*Traces.mainForm.traceForm.form.gadgets.traceName.borderWidth: 0", + "*Traces.mainForm.traceForm.form.gadgets*top: ChainTop", + "*Traces.mainForm.traceForm.form.gadgets*bottom: ChainTop", + "*Traces.mainForm.traceForm.form.gadgets*left: ChainLeft", + "*Traces.mainForm.traceForm.form.gadgets*right: ChainLeft", + "*Traces.mainForm.traceForm.form.gadgets.scaleUp.label: Scale up", + "*Traces.mainForm.traceForm.form.gadgets.scaleDown.label: Scale down", + + + /* Tag Editor */ + /* ---------- */ + "*Tag.mainForm. defaultDistance: 0", + "*Tag.mainForm.buttons.borderWidth: 0", + "*Tag.mainForm.buttons*resize: True", + "*Tag.mainForm.buttons.orientation: horizontal", + "*Tag.mainForm.buttons*top: ChainTop", + "*Tag.mainForm.buttons*bottom: ChainTop", + "*Tag.mainForm.buttons*left: ChainLeft", + "*Tag.mainForm.buttons*right: ChainLeft", + "*Tag.mainForm.tagText.width: 300", + "*Tag.mainForm.tagText.height: 100", + "*Tag.mainForm.tagText.left: ChainLeft", + "*Tag.mainForm.tagText.right: ChainRight", + "*Tag.mainForm.tagText.top: ChainTop", + "*Tag.mainForm.tagText.bottom: ChainBottom", + "*Tag.mainForm.tagText.resize: True", + + NULL, +}; + + + + +/* ---- Callback routines ---- */ + + +static void destroyCallback(Widget w, XtPointer client_data, XtPointer call_data) +{ lifeForce = Dead; +} + + + + +/* ---- Other functions ---- */ + + +static void usage(int argc, char **argv) +{ int i; + + fprintf(stderr, "%s: unknown options:", argv[0]); + for (i=1; i +#include +#include +#include +#include +#include +#include +#include + +/* ---- Types ---- */ + + +typedef struct +{ String name; /* Name of function */ + int number; /* Number of function */ +} MenuData, *MenuDataList; + +/* ---- Static variables ---- */ + + +static Widget menubarWid = (Widget)NULL; /* Initialised by CreateProgMenus */ +static int num_menus = 0; + + +/* --- Callback functions ---- */ + +static XtCallbackProc externalCallbackProc; +static XtPointer externalClient_data; + +static void MenuSelectCallback(Widget w, XtPointer i, XtPointer junk) +/* + Pass the menu item callback back to `externalCallbackProc' + which the user supplied to `CreateProgMenus'. +*/ +{ externalCallbackProc(w, externalClient_data, i); +} + +/* ---- Private functions ---- */ + +static void CreateMenu(Widget parentWid, String menuButtonName, + menuarr *md, int num_md) +{ Widget buttonWid, menuWid; + int i; + + /* + Create the (empty) menu button. + */ + buttonWid = XtCreateManagedWidget(menuButtonName, menuButtonWidgetClass, + parentWid, NULL, 0); + menuWid = XtCreatePopupShell("menu", simpleMenuWidgetClass, buttonWid, + NULL, 0); + + /* + Put the individual items in. + When selected, each entry will generate a callback with + its associated number. + */ + for (i = 0; i < (int) num_md ; i++) { + /* Don't want help/quit/menus in our X menus */ + Widget entryWid; + + if (md[i].number < 3) + continue; + entryWid = XtCreateManagedWidget(md[i].name, smeBSBObjectClass, + menuWid, NULL, 0); + XtAddCallback(entryWid, XtNcallback, MenuSelectCallback, + (XtPointer) md[i].number); + } + +} + + +/* ---- Exported functions ---- */ + +void CreateProgMenus(Widget parentWid, + XtCallbackProc cbp, XtPointer client_data) +/* + Install the menus for this program into `parentWid'. + They are initially all unmanaged. + When pressed, each item (which is the name of a function) will + call `cbp' providing `client_data' and the number of the function + as `call_data'. +*/ +{ + int i, menulen; + menuarr menu[MAXOPTS]; + + externalCallbackProc = cbp; + externalClient_data = client_data; + menubarWid = parentWid; + + for (i = 1; iname, menu, menulen); + } + num_menus = i; +} diff --git a/src/staden/xmepSpec.c b/src/staden/xmepSpec.c new file mode 100644 index 0000000..395126b --- /dev/null +++ b/src/staden/xmepSpec.c @@ -0,0 +1,221 @@ +/* + Title: xmepSpec + + File: xmepSpec.c + Purpose: Definition specific to `xmep' + Last update: Wed Jun 20 1990 +*/ + + +/* + This module contains data specific to the menus and help system + for the `xmep' program. +*/ + + + + +/* ---- Includes ---- */ + +#include "progSpec.h" + +#include +#include +#include +#include +#include +#include +#include + + + + + + +/* ---- Types ---- */ + + +typedef struct +{ String name; /* Name of function */ + int number; /* Number of function */ +} MenuData, *MenuDataList; + + + + +/* ---- Static variables ---- */ + + +static MenuData general_menu_data[] = +{ {"Read new sequence", 3}, + {"Redefine active region", 4}, + {"List the sequences", 5}, + {"List text file", 6}, + {"Direct text output to disc", 7}, + {"Search for strings", 17}, + {"Compare a sequence", 18}, + {"Find inverted repeats", 33}, +}; + + +static MenuData screen_menu_data[] = +{ {"Clear graphics", 10}, + {"Clear text", 11}, + {"Draw a ruler", 12}, + {"Use cross hair", 13}, + {"Reset margins", 14}, + {"Label diagram", 15}, + {"Draw map", 16}, +}; + + +static MenuData analysis_menu_data[] = +{ {"Set strand", 18}, + {"Set composition", 19}, + {"Set word mask", 20}, + {"Set number of mismatches", 21}, + {"Show settings", 22}, + {"Make dictionary Dw", 23}, + {"Make dictionary Ds", 24}, + {"Make fuzzy dictionary Dm from Dw", 25}, + {"Make fuzzy dictionary Dm from Ds", 26}, + {"Make fuzzy dictionary Dh from Dm", 27}, + {"Examine fuzzy dictionary Dm", 28}, + {"Examine fuzzy dictionary Dh", 29}, + {"Examine words in Dm", 30}, + {"Examine words in Dh", 31}, + {"Save or restore a dictionary", 32}, +}; + + + + +/* ---- Callback functions ---- */ + + +static XtCallbackProc externalCallbackProc; +static XtPointer externalClient_data; + +static void MenuSelectCallback(Widget w, XtPointer i, XtPointer junk) +/* + Pass the menu item callback back to `externalCallbackProc' + which the user supplied to `CreateProgMenus'. +*/ +{ externalCallbackProc(w, externalClient_data, i); +} + + + + + +/* ---- Private functions ---- */ + + +static void CreateMenu(Widget parentWid, String menuButtonName, + MenuDataList md, Cardinal num_md) +{ Widget buttonWid, menuWid; + int i; + + /* + Create the (empty) menu button + */ + buttonWid = XtCreateManagedWidget(menuButtonName, menuButtonWidgetClass, + parentWid, NULL, 0); + menuWid = XtCreatePopupShell("menu", simpleMenuWidgetClass, buttonWid, + NULL, 0); + + /* + Put the individual items in. + When selected, each entry will generate a callback with + its associated number. + */ + for (i = 0; i < (int) num_md ; i++) + { Widget entryWid = XtCreateManagedWidget(md[i].name, smeBSBObjectClass, + menuWid, NULL, 0); + XtAddCallback(entryWid, XtNcallback, MenuSelectCallback, + (XtPointer) md[i].number); + } + +} + + + + +/* ---- Exported functions ---- */ + + +void CreateProgMenus(Widget parentWid, + XtCallbackProc cbp, XtPointer client_data) +/* + Install the menus for this program into `parentWid'. + When pressed, each item (which is the name of a function) will + call `cbp' providing `client_data' and the number of the function + as `call_data'. +*/ +{ externalCallbackProc = cbp; + externalClient_data = client_data; + + CreateMenu(parentWid, "General", + general_menu_data, XtNumber(general_menu_data)); + CreateMenu(parentWid, "Screen", + screen_menu_data, XtNumber(screen_menu_data)); + CreateMenu(parentWid, "Dictionary analysis", + analysis_menu_data, XtNumber(analysis_menu_data)); +} + + + + +const int botHelpOpt = 0; +const int topHelpOpt = 35; +/* + The range of option numbers for the help system. +*/ + + +const char helpTextFN[] = "MEPHELP"; +const char helpPtrsFN[] = "MEPHPNT"; +/* + File names for the help text and pointer files. +*/ + + +const char *helpTopics[] = +{ "NIP", + "Help", + "Quit", + "Read new sequences", + "Redefine active region", + "List the sequences", + "List text file", + "Direct output to disk", + "Clear graphics", + "Clear text", + "Draw ruler", + "Use cross hair", + "Reset margins", + "Label diagram", + "Draw map", + "Search for strings", + "Set strand", + "Set composition", + "Set word length", + "Set number of mismatches", + "Show settings", + "Make dictionary Dw", + "Make dictionary Ds", + "Make fuzzy dictionary Dm from Dw", + "Make fuzzy dictionary Dm from Ds", + "Make fuzzy dictionary Dh from Dm", + "Examine fuzzy dictionary Dm", + "Examine fuzzy dictionary Dh", + "Examine words in Dm", + "Examine words in Dh", + "Save or restore a dictionary", + "Find inverted repeats", + NULL, +}; +/* + Help topics, indexed in C between 0 and topHelpOpt-botHelpOpt + but referring to topics botHelpOpt to topHelpOpt +*/ diff --git a/src/staden/xnipSpec.c b/src/staden/xnipSpec.c new file mode 100644 index 0000000..65402e5 --- /dev/null +++ b/src/staden/xnipSpec.c @@ -0,0 +1,321 @@ +/* + Title: xnipSpec + + File: xnipSpec.c + Purpose: Definitions specific to `xnip' + Last update: Tue May 19 1990 +*/ + + +/* + This module contains the setup for the menus for the `xnip' program + and also information needed for `xnip' help. +*/ + + + + +/* ---- Includes ---- */ + +#include "progSpec.h" + +#include +#include +#include +#include +#include +#include +#include + + + + +/* ---- Types ---- */ + + +typedef struct +{ String name; /* IMPORT: Name of function */ + int number; /* IMPORT: Number of function */ +} MenuData, *MenuDataList; + + + + +/* ---- Static variables ---- */ + + +static MenuData general_menu_data[] = +{ {"Read new sequence", 3}, + {"Redefine active region", 4}, + {"List the sequence", 5}, + {"List a text file", 6}, + {"Direct text output to disc", 7}, + {"Write active sequence to disc", 8}, + {"Edit the sequence", 9}, + {"Restriction enzyme search", 17}, + {"Compare a sequence", 18}, + {"Reverse and complement the sequence", 29}, + {"Interconvert t and u", 66}, +}; + + +static MenuData screen_menu_data[] = +{ {"Clear graphics", 10}, + {"Clear text", 11}, + {"Draw a ruler", 12}, + {"Use cross hair", 13}, + {"Reposition plots", 14}, + {"Label diagram", 15}, + {"Display a map", 16}, +}; + + +static MenuData statistics_menu_data[] = +{ {"Count base composition", 21}, + {"Count dinucleotide frequencies", 22}, + {"Count codons and amino acids", 23}, + {"Plot base composition", 24}, + {"Plot local deviations in base composition", 25}, + {"Plot local deviations in dinucleotide composition", 26}, + {"Plot local deviations in trinucleotide composition", 27}, + {"Plot negentropy", 59}, + {"Plot observed-expected word frequencies", 64}, +}; + + +static MenuData structure_menu_data[] = +{ {"Search for hairpin loops", 30}, + {"Search for long range inverted repeats", 31}, + {"Search for repeats", 32}, + {"Examine repeats", 38}, + {"Search for z dna(total ry,yr)", 33}, + {"Search for z dna(runs of ry or yr", 34}, + {"Search for z dna(best phased value)", 35}, + {"Find local similarity or complementarity", 36}, +}; + + +static MenuData translation_menu_data[] = +{ {"Set genetic code", 37}, + {"Translate and list in up to six phases", 39}, + {"Translate and write protein sequence to disc", 40}, + {"Calculate codon constraint", 28}, + {"Count codons and amino acids", 23}, + {"Write codon table to disk", 41}, + {"Search for open reading frames", 54}, +}; + + +static MenuData gene_menu_data[] = +{ {"Codon usage", 42}, + {"Positional base preferences", 43}, + {"Uneven positional base frequencies", 44}, + {"Codon improbability (on base composition)", 45}, + {"Codon improbability (on amino acid composition)", 46}, + {"Shepherd RNY preference", 47}, + {"Fickett testcode", 48}, + {"tRNA gene search", 49}, +}; + + +static MenuData specific_signals_menu_data[] = +{ {"Plot e. coli promters (general)", 55}, + {"Plot e. coli promters (complementary)", 56}, + {"Plot e. coli promters (-35 and -10)", 57}, + {"Plot e. coli ribosome binding sites", 58}, + {"Plot eukaryotic ribosome binding sites", 61}, + {"Plot splice junctions", 62}, + {"Plot polya sites", 65}, +}; + + +static MenuData general_signals_menu_data[] = +{ {"Compare a short sequence", 18}, + {"Compare a sequence using a score matrix", 19}, + {"Search using a weight matrix", 20}, + {"Search using a weight matrix (complementary)", 63}, + {"Search using a dinucleotide weight matrix", 60}, + {"Plot start codons", 50}, + {"Plot stop codons", 51}, + {"Plot complementary stop codons", 52}, + {"Plot stop codons on both strands", 53}, + {"Search for patterns of motifs", 67}, +}; + + + + +/* --- Callback functions ---- */ + +static XtCallbackProc externalCallbackProc; +static XtPointer externalClient_data; + +static void MenuSelectCallback(Widget w, XtPointer i, XtPointer junk) +/* + Pass the menu item callback back to `externalCallbackProc' + which the user supplied to `CreateProgMenus'. +*/ +{ externalCallbackProc(w, externalClient_data, i); +} + + + + +/* ---- Private functions ---- */ + + +static void CreateMenu(Widget parentWid, String menuButtonName, + MenuDataList md, Cardinal num_md) +{ Widget buttonWid, menuWid; + int i; + + /* + Create the (empty) menu button + */ + buttonWid = XtCreateManagedWidget(menuButtonName, menuButtonWidgetClass, + parentWid, NULL, 0); + menuWid = XtCreatePopupShell("menu", simpleMenuWidgetClass, buttonWid, + NULL, 0); + + /* + Put the individual items in. + When selected, each entry will generate a callback with + its associated number. + */ + for (i = 0; i < (int) num_md ; i++) + { Widget entryWid = XtCreateManagedWidget(md[i].name, smeBSBObjectClass, + menuWid, NULL, 0); + XtAddCallback(entryWid, XtNcallback, MenuSelectCallback, + (XtPointer) md[i].number); + } + +} + + + + +/* ---- Exported functions ---- */ + + + +void CreateProgMenus(Widget parentWid, + XtCallbackProc cbp, XtPointer client_data) +/* + Install the menus for this program into `parentWid'. + When pressed, each item (which is the name of a function) will + call `cbp' providing `client_data' and the number of the function + as `call_data'. +*/ +{ externalCallbackProc = cbp; + externalClient_data = client_data; + + CreateMenu(parentWid, "General", + general_menu_data, XtNumber(general_menu_data)); + CreateMenu(parentWid, "Screen", + screen_menu_data, XtNumber(screen_menu_data)); + CreateMenu(parentWid, "Statistics", + statistics_menu_data, XtNumber(statistics_menu_data)); + CreateMenu(parentWid, "Structure", + structure_menu_data, XtNumber(structure_menu_data)); + CreateMenu(parentWid, "Translation and codons", + translation_menu_data, XtNumber(translation_menu_data)); + CreateMenu(parentWid, "Gene search by content", + gene_menu_data, XtNumber(gene_menu_data)); + CreateMenu(parentWid, "General signals menu", + general_signals_menu_data, XtNumber(general_signals_menu_data)); + CreateMenu(parentWid, "Specific signals menu", + specific_signals_menu_data, XtNumber(specific_signals_menu_data)); +} + + + + +const int botHelpOpt = -10; +const int topHelpOpt = 70; +/* + The range of option numbers for the help system. +*/ + + +const char helpTextFN[] = "NIPHELP"; +const char helpPtrsFN[] = "NIPHPNT"; +/* + File names for the help text and pointer files. +*/ + + +const char *helpTopics[] = +{ "NIP", + "HELP", + "Quit", + "read a new sequence", + "define active region", + "list the sequence", + "list a text file", + "direct output to disk", + "write active sequence to disk", + "edit the sequence", + "clear graphics screen", + "clear text screen", + "draw a ruler", + "use cross hair", + "reposition plots", + "label diagram", + "display a map", + "restriction enzyme search", + "compare a sequence", + "compare a sequence using a score matrix", + "search using a weight matrix", + "count base frequencies", + "count dinucleotide frequencies", + "count codons and amino acids", + "plot base composition", + "plot deviations in base composition", + "plot deviations in dinucleotide composition", + "plot deviations in trinucleotide composition", + "calculate codon constraint", + "plot negentropy", + "search for hairpin loops", + "search for long range inverted repeats", + "search for repeats", + "search for Z DNA (total RY, YR)", + "search for Z DNA (runs of RY or YR)", + "search for Z DNA (best phased value)", + "find local similarity or complementarity", + "set genetic code", + "examine repeats", + "translate and list in 6 phases", + "translate and write protein to disk", + "calculate and write codon table to disk", + "gene search using codon preference", + "gene search using positional base preferences", + "gene search using uneven positional base frequencies", + "gene search using codon improbability (on base composition)", + "gene search using codon improbability (on amino acid composition)", + "gene search using RNY preference", + "gene search using Ficketts method", + "tRNA gene search", + "plot start codons", + "plot stop codons", + "plot complementary stop codons", + "plot stop codons on both strands", + "find longest open reading frames", + "plot E. coli promoters (general)", + "plot E. coli promoters (complementary)", + "plot E. coli promoters (-5 and -10 separate)", + "plot E. coli ribosome binding sites", + "reverse and complement the active sequence", + "universal signal plot (on dinucleotides)", + "plot eukaryotic ribosome binding sites", + "plot splice junctions", + "search the complementary strand using a weight matrix", + "plot observed-expected word frequencies", + "plot polyA sites", + "interconvert T and U", + "search for patterns of motifs", + NULL, +}; +/* + Help topics, indexed in C between 0 and topHelpOpt-botHelpOpt + but referring to topics botHelpOpt to topHelpOpt +*/ diff --git a/src/staden/xpipSpec.c b/src/staden/xpipSpec.c new file mode 100644 index 0000000..a561299 --- /dev/null +++ b/src/staden/xpipSpec.c @@ -0,0 +1,228 @@ +/* + Title: xpipSpec + + File: xpipSpec.c + Purpose: Definitions specific to `xpip' + Last update: Wed Jun 20 1990 +*/ + + +/* + This module contains data specific to the menus and help system + for the `xpip' program. +*/ + + + + +/* ---- Includes ---- */ + +#include "progSpec.h" + +#include +#include +#include +#include +#include +#include +#include + + + + +/* ---- Types ---- */ + + +typedef struct +{ String name; /* Name of function */ + int number; /* Number of function */ +} MenuData, *MenuDataList; + + + + +/* ---- Static variables ---- */ + + +static MenuData general_menu_data[] = +{ {"Read new sequence", 3}, + {"Redefine active region", 4}, + {"List the sequence", 5}, + {"List a text file", 6}, + {"Direct text output to disc", 7}, + {"Write active sequence to disc", 8}, + {"Edit the sequence", 9}, + {"Short sequence search", 17}, + {"Compare a sequence", 18}, + {"Compare a sequence using a score matrix", 19}, + {"Back translate to dna", 27}, +}; + + +static MenuData screen_menu_data[] = +{ {"Clear graphics", 10}, + {"Clear text", 11}, + {"Draw a ruler", 12}, + {"Use cross hair", 13}, + {"Reset margins", 14}, + {"Label diagram", 15}, + {"Display a map", 16}, +}; + + +static MenuData statistics_menu_data[] = +{ {"Count amino acid composition", 21}, + {"Plot hydrophobicity", 22}, + {"Plot charge", 23}, + {"Plot hydrophobic moment", 25}, +}; + + +static MenuData structure_menu_data[] = +{ {"Plot hydrophobicity", 22}, + {"Plot charge", 23}, + {"Plot robson prediction", 24}, + {"Plot hydrophobic moment", 25}, + {"Draw helix wheel", 26}, +}; + + +static MenuData search_menu_data[] = +{ {"Search for short sequences", 17}, + {"Compare a sequence", 18}, + {"Compare a sequence using a score matrix", 19}, + {"Search for a sequence using a weight matrix", 20}, + {"Search for patterns of motifs", 28}, +}; + + + + +/* --- Callback functions ---- */ + +static XtCallbackProc externalCallbackProc; +static XtPointer externalClient_data; + +static void MenuSelectCallback(Widget w, XtPointer i, XtPointer junk) +/* + Pass the menu item callback back to `externalCallbackProc' + which the user supplied to `CreateProgMenus'. +*/ +{ externalCallbackProc(w, externalClient_data, i); +} + + + + +/* ---- Private functions ---- */ + + +static void CreateMenu(Widget parentWid, String menuButtonName, + MenuDataList md, Cardinal num_md) +{ Widget buttonWid, menuWid; + int i; + + /* + Create the (empty) menu button + */ + buttonWid = XtCreateManagedWidget(menuButtonName, menuButtonWidgetClass, + parentWid, NULL, 0); + menuWid = XtCreatePopupShell("menu", simpleMenuWidgetClass, buttonWid, + NULL, 0); + + /* + Put the individual items in. + When selected, each entry will generate a callback with + its associated number. + */ + for (i = 0; i < (int) num_md ; i++) + { Widget entryWid = XtCreateManagedWidget(md[i].name, smeBSBObjectClass, + menuWid, NULL, 0); + XtAddCallback(entryWid, XtNcallback, MenuSelectCallback, + (XtPointer) md[i].number); + } + +} + + + + +/* ---- Exported functions ---- */ + + +void CreateProgMenus(Widget parentWid, + XtCallbackProc cbp, XtPointer client_data) +/* + Install the menus for this program into `parentWid'. + When pressed, each item (which is the name of a function) will + call `cbp' providing `client_data' and the number of the function + as `call_data'. +*/ +{ externalCallbackProc = cbp; + externalClient_data = client_data; + + CreateMenu(parentWid, "General", + general_menu_data, XtNumber(general_menu_data)); + CreateMenu(parentWid, "Screen", + screen_menu_data, XtNumber(screen_menu_data)); + CreateMenu(parentWid, "Statistics", + statistics_menu_data, XtNumber(statistics_menu_data)); + CreateMenu(parentWid, "Structure", + structure_menu_data, XtNumber(structure_menu_data)); + CreateMenu(parentWid, "Search", + search_menu_data, XtNumber(search_menu_data)); +} + + + + +const int botHelpOpt = 0; +const int topHelpOpt = 28; +/* + The range of option numbers for the help system. +*/ + + +const char helpTextFN[] = "PIPHELP"; +const char helpPtrsFN[] = "PIPHPNT"; +/* + File names for the help text and pointer files. +*/ + + +const char *helpTopics[] = +{ "PIP", + "Help", + "Quit", + "read a new sequence", + "define active region", + "list the sequence", + "list a text file", + "direct output to disk", + "write active sequence to disk", + "edit the sequence", + "clear graphics screen", + "clear text screen", + "draw a ruler", + "use cross hair", + "reposition plots", + "label diagram", + "display a map", + "search for short sequences", + "compare a sequence", + "compare a sequence using a score matrix", + "search for a sequence using a weight matrix", + "calculate amino acid composition", + "plot hydrophobicity", + "plot charge", + "plot Robson prediction", + "plot hydrophobic moment", + "draw helix wheel", + "back translate", + "search for patterns of motifs", + NULL, +}; +/* + Help topics, indexed in C between 0 and topHelpOpt-botHelpOpt + but referring to topics botHelpOpt to topHelpOpt +*/ diff --git a/src/staden/xsapConEdit.f b/src/staden/xsapConEdit.f new file mode 100644 index 0000000..b1fa0f5 --- /dev/null +++ b/src/staden/xsapConEdit.f @@ -0,0 +1,137 @@ +C 1-10-91 SD Remove prompting from CONEDT and JOINED +C 1-10-91 SD Removed LLINOL and LLINOR from DOJOIN +C 20-Aug-92 SD Added new IOK argument to CXEDIT and JXEDIT call +C + SUBROUTINE CONEDT(KBIN,KBOUT, + +GELNOS,GELSTR,GELEND, + +MAXDB,GELNO,LINNO,MAXLIN,RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,NGELS,NCONTS,GEL,GEL2,MAXGEL,LINCON,PERCD,IDM, + +IHELPS,IHELPE,HELPF,IDEVH,IDEV,IDEV1,IDEV2,IDEV3, + +IDEVT,IDEVC,LINLEN,FILNAM,LLINO,IOK,IERR,TEMP1) +C AUTHOR: SIMON DEAR + INTEGER RELPG(MAXDB),LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER GEL(MAXGEL),GEL2(MAXGEL) + CHARACTER FILNAM*(*),HELPF*(*) + INTEGER GELNOS(MAXDB),GELSTR(MAXDB),GELEND(MAXDB) + INTEGER GELNO(MAXLIN,2),LINNO(MAXLIN,2) + CALL SHOWFU(KBOUT,'Contig editor') +C WRITE(KBOUT,*)'Identify contig to edit' + CALL GETLN2(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LINCON, + +LLINO,IGELNO,IOK,IDBSIZ,KBIN,KBOUT,IDEV3, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.NE.0) RETURN + CALL CXEDIT(IDEV1,IDEV2,IDEV3,IDEVT,IDEVC, + +RELPG,LNGTHG,LNBR,RNBR,MAXGEL, + +IDBSIZ,LINCON,LLINO,IGELNO,1,PERCD,IDM,0,IOK) + CALL DBCHEK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + +TEMP1,IERR,KBOUT) + END + SUBROUTINE JOINED(KBIN,KBOUT, + +GELNOS,GELSTR,GELEND, + +MAXDB,GELNO,LINNO,MAXLIN,RELPG,LNGTHG,LNBR,RNBR, + +IDBSIZ,NGELS,NCONTS,GEL,GEL2,MAXGEL,LINCON,PERCD,IDM, + +IHELPS,IHELPE,HELPF,IDEVH,IDEV,IDEV1,IDEV2,IDEV3, + +IDEVT,IDEVC,LINLEN,FILNAM,LLINO,IOK,IERR,TEMP1) +C AUTHOR: SIMON DEAR + INTEGER RELPG(MAXDB),LNGTHG(MAXDB),LNBR(MAXDB),RNBR(MAXDB) + CHARACTER GEL(MAXGEL),GEL2(MAXGEL) + CHARACTER FILNAM*(*),HELPF*(*) + INTEGER GELNOS(MAXDB),GELSTR(MAXDB),GELEND(MAXDB) + INTEGER GELNO(MAXLIN,2),LINNO(MAXLIN,2) +C JOIN + CALL SHOWFU(KBOUT,'Join editor') +C JOIN + WRITE(KBOUT,1006) +1006 FORMAT(' Which contigs do you want to join ?') +C WRITE(KBOUT,1017) +C1017 FORMAT( ' Left contig') + LLINOL = 0 + LLINOR = 0 + CALL GETLN2(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LNCONL, + +LLINOL,IGELL,IOK,IDBSIZ,KBIN,KBOUT,IDEV3, + +IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.EQ.0)THEN +C WRITE(KBOUT,1018) +C1018 FORMAT( ' Right contig') + CALL GETLN2(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,LNCONR, + + LLINOR,IGELR,IOK,IDBSIZ,KBIN,KBOUT,IDEV3, + + IHELPS,IHELPE,HELPF,IDEVH) + IF(IOK.EQ.0)THEN +C CHECK TO SEE IF MAKING A CIRCLE! + IF(LLINOL.EQ.LLINOR)THEN + WRITE(KBOUT,*)' Making circles is not allowed!' + GO TO 200 + END IF + CALL JXEDIT(IDEV1,IDEV2,IDEV3,IDEVT,IDEVC, + + RELPG,LNGTHG,LNBR,RNBR,MAXGEL, + + IDBSIZ,LNCONL,LLINOL,LNCONR,LLINOR, + + IGELL,1,IGELR,1,PERCD,NGELS,NCONTS,IDM,0,IOK) + CALL DBCHEK(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS,IDBSIZ, + + TEMP1,IERR,KBOUT) + END IF + END IF +200 CONTINUE + END + SUBROUTINE DOJOIN(RELPG,LNGTHG,LNBR,RNBR,NGELS,NCONTS, + +LNCONL,LNCONR, + +IDBSIZ,IDEVR,IDEVW, + +RELX) +C AUTHOR: RODGER STADEN +C TAKEN FROM: JOIN + INTEGER LLINOL,LLINOR + INTEGER RELPG(IDBSIZ) + INTEGER X,RELX + INTEGER LNGTHG(IDBSIZ),RNBR(IDBSIZ),LNBR(IDBSIZ) +C SET UP LLINOL, LLINOR + LLINOL = LNBR(LNCONL) + LLINOR = LNBR(LNCONR) +C COMPLETE JOIN +C ADJUST ALL RELATIVE POSITIONS IN RIGHT CONTIG + N=LLINOR + RELPG(N)=RELX +50 CONTINUE + IF(RNBR(N).EQ.0)GO TO 60 + N=RNBR(N) + RELPG(N)=RELPG(N)+RELX-1 + GO TO 50 +60 CONTINUE +C +C FIX UP NEW GEL LINE FOR OLD LEFT OF RIGHT CONTIG + LNBR(LLINOR)=RNBR(LNCONL) +C FIX UP RIGHT GEL OF LEFT CONTIG + N=RNBR(LNCONL) + RNBR(N)=LLINOR + CALL MERGE(RELPG,LNGTHG,LNBR,RNBR,LNCONL,IDBSIZ) +C MERGE DOES NOT WRITE TO DISK + N=LNBR(LNCONL) +65 CONTINUE + CALL WRITER(IDEVR,N,RELPG(N),LNGTHG(N), + +LNBR(N),RNBR(N)) + N=RNBR(N) + IF(N.NE.0)GO TO 65 +C CONTIG LINES + X=RELPG(LNCONR)+RELX-1 + IF(X.GT.RELPG(LNCONL))RELPG(LNCONL)=X + CALL WRITER(IDEVR,LNCONL,RELPG(LNCONL),LNGTHG(LNCONL), + +LNBR(LNCONL),RNBR(LNCONL)) +C NOW MOVE ALL DATA DOWN TO DELETE OLD RIGHT END + N=IDBSIZ-NCONTS + M=LNCONR-N + IF(M.EQ.0)GO TO 80 + K=LNCONR + J=LNCONR-1 + DO 70 I=1,M + RELPG(K)=RELPG(J) + LNGTHG(K)=LNGTHG(J) + LNBR(K)=LNBR(J) + RNBR(K)=RNBR(J) + CALL WRITER(IDEVR,K,RELPG(K),LNGTHG(K), + +LNBR(K),RNBR(K)) + K=K-1 + J=J-1 +70 CONTINUE +80 CONTINUE + NCONTS=NCONTS-1 + CALL WRITER(IDEVR,IDBSIZ,NGELS,NCONTS,NGELS,NCONTS) + RETURN + END diff --git a/src/staden/xsapSpec.c b/src/staden/xsapSpec.c new file mode 100644 index 0000000..400272f --- /dev/null +++ b/src/staden/xsapSpec.c @@ -0,0 +1,416 @@ +/* + Title: xsapSpec + + File: xsapSpec.c + Purpose: Definitions specific to the `xsap' program + Last update: Mon Jun 25 1990 +*/ + + +/* + This module contains data specific to the menus and help system + for the `xsap' program. +*/ + + + + +/* ---- Includes ---- */ + +#include "fort.h" +#include "progSpec.h" +#include "userface.h" /* IMPORT: menu_x */ + +#include +#include +#include +#include +#include +#include +#include + + + + +/* ---- Types ---- */ + + +typedef struct +{ String name; /* Name of function */ + int number; /* Number of function */ +} MenuData, *MenuDataList; + + + + +/* ---- Static variables ---- */ + + +static Widget menubarWid = (Widget)NULL; /* Initialised by CreateProgMenus */ + + +static MenuData general_menu_data[] = +{ {"Open a database", 3}, + {"Display a contig", 5}, + {"List a text file", 6}, + {"Redirect output", 7}, + {"Calculate a consensus", 8}, + {"Screen against restriction enzymes", 17}, + {"Screen against vector", 18}, + {"Check database", 19}, + {"Copy database", 24}, + {"Show relationships", 25}, + {"Set parameters", 27}, + {"Highlight disagreements", 28}, + {"Examine quality", 29}, + {"Find internal joins", 35}, +}; + + +static MenuData screen_menu_data[] = +{ {"Clear graphics", 10}, + {"Clear text", 11}, + {"Draw ruler", 12}, + {"Use cross hair", 13}, + {"Change margins", 14}, + {"Label diagram", 15}, + {"Plot map", 16}, + {"Plot single contig", 33}, + {"Plot all contigs", 34}, +}; + + +static MenuData modification_menu_data[] = +{ {"Edit contig", 4}, + {"Screen edit", 9}, + {"Auto assemble", 20}, + {"Enter new gel reading", 21}, + {"Join contigs", 22}, + {"Complement a contig", 23}, + {"Alter relationships", 26}, + {"Auto edit a contig", 30}, + {"Type in gel readings", 31}, + {"Extract gel readings", 32}, +}; + + +static MenuData enter_menu_data[] = +{ {"Cancel", 2}, + {"Complete entry", 3}, + {"Edit contig", 4}, + {"Display", 5}, + {"Edit new gel reading", 6}, +}; + + +static MenuData join_menu_data[] = +{ {"Cancel", 2}, + {"Complete join", 3}, + {"Edit left contig", 4}, + {"Display join", 5}, + {"Edit right contig", 6}, + {"Move join", 7}, +}; + + +static MenuData alter_menu_data[] = +{ {"Cancel", 2}, + {"Line change", 3}, + {"Edit gel reading", 4}, + {"Delete contig", 5}, + {"Shift", 6}, + {"Move gel reading", 7}, + {"Rename gel reading", 8}, + {"Break a contig", 9}, +}; + + +static MenuData edit_menu_data[] = +{ {"Cancel", 2}, + {"Insert", 3}, + {"Delete", 4}, + {"Change", 5}, +}; + + + + +/* --- Callback functions ---- */ + +static XtCallbackProc externalCallbackProc; +static XtPointer externalClient_data; + +static void MenuSelectCallback(Widget w, XtPointer i, XtPointer junk) +/* + Pass the menu item callback back to `externalCallbackProc' + which the user supplied to `CreateProgMenus'. +*/ +{ externalCallbackProc(w, externalClient_data, i); +} + + + + +/* ---- Private functions ---- */ + + +static void CreateMenu(Widget parentWid, String menuButtonName, + MenuDataList md, Cardinal num_md) +{ Widget buttonWid, menuWid; + int i; + + /* + Create the (empty) menu button. + The button is initially unmanaged within its parent. + */ + buttonWid = XtCreateWidget(menuButtonName, menuButtonWidgetClass, + parentWid, NULL, 0); + menuWid = XtCreatePopupShell("menu", simpleMenuWidgetClass, buttonWid, + NULL, 0); + + /* + Put the individual items in. + When selected, each entry will generate a callback with + its associated number. + */ + for (i = 0; i < (int) num_md ; i++) + { Widget entryWid = XtCreateManagedWidget(md[i].name, smeBSBObjectClass, + menuWid, NULL, 0); + XtAddCallback(entryWid, XtNcallback, MenuSelectCallback, + (XtPointer) md[i].number); + } + +} + + + + +/* ---- Exported functions ---- */ + + +void CreateProgMenus(Widget parentWid, + XtCallbackProc cbp, XtPointer client_data) +/* + Install the menus for this program into `parentWid'. + They are initially all unmanaged. + When pressed, each item (which is the name of a function) will + call `cbp' providing `client_data' and the number of the function + as `call_data'. +*/ +{ externalCallbackProc = cbp; + externalClient_data = client_data; + menubarWid = parentWid; + + CreateMenu(parentWid, "General", + general_menu_data, XtNumber(general_menu_data)); + CreateMenu(parentWid, "Screen", + screen_menu_data, XtNumber(screen_menu_data)); + CreateMenu(parentWid, "Modification", + modification_menu_data, XtNumber(modification_menu_data)); + CreateMenu(parentWid, "Enter", + enter_menu_data, XtNumber(enter_menu_data)); + CreateMenu(parentWid, "Join", + join_menu_data, XtNumber(join_menu_data)); + CreateMenu(parentWid, "Alter", + alter_menu_data, XtNumber(alter_menu_data)); + CreateMenu(parentWid, "Edit", + edit_menu_data, XtNumber(edit_menu_data)); +} + + + + +/* + Special menu functions for SAP. + + The generic menu functions, found in 'dialogues' are only + used indirectly by SAP. + + SUBROUTINE DBMENT(MENU,NOPT,MAXOPT, + +IHELPS,IHELPE,HELPF,IDEVH,KBIN,KBOUT) + CHARACTER HELPF*(*) + INTEGER IHELPS(0:MAXOPT),IHELPE(0:MAXOPT) + + SUBROUTINE DBMENU(MENU,NOPT,IHELPS,IHELPE,HELPF,IDEVH, + +KBIN,KBOUT) + CHARACTER HELPF*(*) + INTEGER IHELPS,IHELPE + + If this code were in 'dialogues', then we wouldn't need to + keep the reference to 'menubarWid' which is making a big + assumption anyway. +*/ + +#define dbment_x dbment_ +#define dbmenu_x dbmenu_ + + +void dbment_x(int_f *MENU_p, + int_f *NOPT_p, + int_f *MAXOPT_p, + int_f *IHELPS_p, + int_f *IHELPE_p, + char *HELPF_p, + int_f *IDEVH_p, + int_f *KBIN_p, + int_f *KBOUT_p, + int_fl HELPF_l) +{ WidgetList sprogs; + int nSprogs; + int_f KOPT, MOPT, MINMEM; /* Dummy arguments to keep menu_x happy */ + Arg args[10]; + int nargs; + + + /* + All the menus are children of 'menubarWid'. + Manage just the 'top level' menus. + */ + nargs = 0; + XtSetArg(args[nargs], XtNchildren, &sprogs); nargs++; + XtSetArg(args[nargs], XtNnumChildren, &nSprogs); nargs++; + XtGetValues(menubarWid, args, nargs); + XtUnmanageChildren(sprogs, nSprogs); + + XtManageChild(XtNameToWidget(menubarWid, "General")); + XtManageChild(XtNameToWidget(menubarWid, "Screen")); + XtManageChild(XtNameToWidget(menubarWid, "Modification")); + + + /* + Run the menu with the usual mechanism. + */ + menu_x( NOPT_p, + &KOPT, + &MOPT, + MAXOPT_p, + &MINMEM, + KBIN_p, + KBOUT_p, + IHELPS_p, + IHELPE_p, + HELPF_p, + IDEVH_p, + HELPF_l); +} + + +void dbmenu_x(int_f *MENU_p, + int_f *NOPT_p, + int_f *IHELPS_p, + int_f *IHELPE_p, + char *HELPF_p, + int_f *IDEVH_p, + int_f *KBIN_p, + int_f *KBOUT_p, + int_fl HELPF_l) +{ WidgetList sprogs; + int nSprogs; + int_f KOPT, MOPT, MINMEM, MAXOPT; /* Dummy arguments to keep menu_x happy */ + Arg args[10]; + int nargs; + + + /* + All the menus are children of 'menubarWid'. + Manage just the menu specified by 'MENU'. + */ + nargs = 0; + XtSetArg(args[nargs], XtNchildren, &sprogs); nargs++; + XtSetArg(args[nargs], XtNnumChildren, &nSprogs); nargs++; + XtGetValues(menubarWid, args, nargs); + XtUnmanageChildren(sprogs, nSprogs); + + switch (*MENU_p) + { case 2: XtManageChild(XtNameToWidget(menubarWid, "Enter")); + break; + + case 3: XtManageChild(XtNameToWidget(menubarWid, "Join")); + break; + + case 4: XtManageChild(XtNameToWidget(menubarWid, "Alter")); + break; + + case 5: XtManageChild(XtNameToWidget(menubarWid, "Edit")); + break; + } + + /* + Run the menu with the usual mechanism. + */ + menu_x( NOPT_p, + &KOPT, + &MOPT, + &MAXOPT, + &MINMEM, + KBIN_p, + KBOUT_p, + IHELPS_p, + IHELPE_p, + HELPF_p, + IDEVH_p, + HELPF_l); + +} + + + + + +const int botHelpOpt = 0; +const int topHelpOpt = 35; +/* + The range of option numbers for the help system. +*/ + + +const char helpTextFN[] = "SAPHELP"; +const char helpPtrsFN[] = "SAPHPNT"; +/* + File names for the help text and pointer files. +*/ + + +const char *helpTopics[] = +{ "SAP", + "Help", + "Quit", + "Open a database", + "Edit contig", + "Display a contig", + "List a text file", + "Direct output to disk", + "Calculate a consensus", + "Screen edit", + "Clear graphics", + "Clear text", + "Draw ruler", + "Use cross hair", + "Change margins", + "Plot map", + "Label diagram", + "Screen against restriction enzymes", + "Screen against vector", + "Check consistency", + "Auto assemble", + "Enter new gel reading", + "Join contigs", + "Complement a contig", + "Copy database", + "Show relationships", + "Alter relationships", + "set parameters", + "Highlight disagreements", + "Examine quality", + "Auto edit a contig", + "Type in gel readings", + "Extract gel readings", + "Plot single contig", + "Plot all contigs", + "Find internal joins", + NULL, +}; +/* + Help topics, indexed in C between 0 and topHelpOpt-botHelpOpt + but referring to topics botHelpOpt to topHelpOpt +*/ diff --git a/src/staden/xsipSpec.c b/src/staden/xsipSpec.c new file mode 100644 index 0000000..83693cc --- /dev/null +++ b/src/staden/xsipSpec.c @@ -0,0 +1,226 @@ +/* + Title: xsipSpec + + File: xsipSpec.c + Purpose: Definitions specific to the `xsip' program + Last update: Tue Jun 19 1990 +*/ + + +/* + This module contains data specific to the menus and help system + for the `xmep' program. +*/ + + + + +/* ---- Includes ---- */ + +#include "progSpec.h" + +#include +#include +#include +#include +#include +#include +#include + + + + +/* ---- Types ---- */ + + +typedef struct +{ String name; /* Name of function */ + int number; /* Number of function */ +} MenuData, *MenuDataList; + + + + +/* ---- Static variables ---- */ + + +static MenuData general_menu_data[] = +{ {"Read new sequence", 3}, + {"Redefine active region", 4}, + {"List the sequences", 5}, + {"List a text file", 6}, + {"Direct text output to disc", 7}, + {"Write active sequence to disc", 8}, + {"Edit the sequences", 9}, + {"Complement sequences", 29}, +}; + + +static MenuData screen_menu_data[] = +{ {"Clear graphics", 10}, + {"Clear text", 11}, + {"Draw a ruler", 12}, + {"Use cross hair", 13}, + {"Reposition plots", 14}, + {"Label diagram", 15}, + {"Display a map", 16}, + {"Draw a /", 27}, +}; + + +static MenuData parameters_menu_data[] = +{ {"Set span length", 20}, + {"Set proportional score", 21}, + {"Set identities score", 22}, + {"Calculate expected scores", 23}, + {"Calculate observed scores", 24}, + {"Show current parameter settings", 25}, + {"Switch main diagonal", 30}, + {"Switch identities", 31}, + {"Change score matrix", 32}, + {"Set number of sd for Quickscan", 33}, + {"Set gap penalties", 34}, +}; + + +static MenuData comparison_menu_data[] = +{ {"Apply identities algorithm", 17}, + {"Apply proportional algorithm", 18}, + {"List matching spans", 19}, + {"Quick scan", 26}, + {"Align sequences", 28}, +}; + + + + +/* --- Callback functions ---- */ + +static XtCallbackProc externalCallbackProc; +static XtPointer externalClient_data; + +static void MenuSelectCallback(Widget w, XtPointer i, XtPointer junk) +/* + Pass the menu item callback back to `externalCallbackProc' + which the user supplied to `CreateProgMenus'. +*/ +{ externalCallbackProc(w, externalClient_data, i); +} + + + + +/* ---- Private functions ---- */ + + +static void CreateMenu(Widget parentWid, String menuButtonName, + MenuDataList md, Cardinal num_md) +{ Widget buttonWid, menuWid; + int i; + + /* + Create the (empty) menu button + */ + buttonWid = XtCreateManagedWidget(menuButtonName, menuButtonWidgetClass, + parentWid, NULL, 0); + menuWid = XtCreatePopupShell("menu", simpleMenuWidgetClass, buttonWid, + NULL, 0); + + /* + Put the individual items in. + When selected, each entry will generate a callback with + its associated number. + */ + for (i = 0; i < (int) num_md ; i++) + { Widget entryWid = XtCreateManagedWidget(md[i].name, smeBSBObjectClass, + menuWid, NULL, 0); + XtAddCallback(entryWid, XtNcallback, MenuSelectCallback, + (XtPointer) md[i].number); + } + +} + + + + +/* ---- Exported functions ---- */ + + +void CreateProgMenus(Widget parentWid, + XtCallbackProc cbp, XtPointer client_data) +/* + Install the menus for this program into `parentWid'. + When pressed, each item (which is the name of a function) will + call `cbp' providing `client_data' and the number of the function + as `call_data'. +*/ +{ externalCallbackProc = cbp; + externalClient_data = client_data; + + CreateMenu(parentWid, "General", + general_menu_data, XtNumber(general_menu_data)); + CreateMenu(parentWid, "Screen", + screen_menu_data, XtNumber(screen_menu_data)); + CreateMenu(parentWid, "Set parameters", + parameters_menu_data, XtNumber(parameters_menu_data)); + CreateMenu(parentWid, "Comparison", + comparison_menu_data, XtNumber(comparison_menu_data)); +} + + + + +const int botHelpOpt = 0; +const int topHelpOpt = 34; +/* + The range of option numbers for the help system. +*/ + + +const char helpTextFN[] = "SIPHELP"; +const char helpPtrsFN[] = "SIPHPNT"; +/* + File names for the help text and pointer files. +*/ + + +const char *helpTopics[] = +{ "SIP", + "Help", + "Quit", + "read a new sequence", + "define active region", + "list the sequence", + "list a text file", + "direct output to disk", + "write active sequence to disk", + "edit the sequences", + "clear graphics screen", + "clear text screen", + "draw a ruler", + "use cross hair", + "reposition plots", + "label diagram", + "display a map", + "apply identities algorithm", + "apply proportional algorithm", + "list matching spans", + "set span length", + "set proportional score", + "set identities score", + "calculate expected scores", + "calculate observed scores", + "show current parameter settings", + "quick scan", + "draw a /", + "align the sequences", + "complement the sequences", + "switch main diagonal", + "switch identities", + "change score matrix", + NULL, +}; +/* + Help topics, indexed in C between 0 and topHelpOpt-botHelpOpt + but referring to topics botHelpOpt to topHelpOpt +*/ diff --git a/src/staden/xspec.f b/src/staden/xspec.f new file mode 100644 index 0000000..d1c0428 --- /dev/null +++ b/src/staden/xspec.f @@ -0,0 +1,11 @@ +C X specific bits +C AUTHOR: RODGER STADEN + SUBROUTINE INITRS() + CALL INITX() + END + INTEGER FUNCTION XVERSN() +C +C set xversn to true for x version +C + XVERSN = 1 + END diff --git a/src/ted/CRN b/src/ted/CRN new file mode 100644 index 0000000..990a9c4 --- /dev/null +++ b/src/ted/CRN @@ -0,0 +1,11 @@ +ted - trace editor + +Copyright 1991 Timothy Gleeson, LaDeana Hillier and Simon Dear + +PLEASE NOTE: + 373A sequence data file formats are the exclusive property + of Applied Biosystems, Inc. + + ALF sequence data file formats are the exclusive property + of Pharmacia LKB Biotechnology, Inc. + diff --git a/src/ted/Graph.c b/src/ted/Graph.c new file mode 100644 index 0000000..0e69bc7 --- /dev/null +++ b/src/ted/Graph.c @@ -0,0 +1,283 @@ +#include + +/* $XConsortium: Graph.c,v 1.2 88/10/25 17:40:25 swick Exp $ */ +/* Copyright Massachusetts Institute of Technology 1987, 1988 */ + +#include +#include +#include "GraphP.h" + + +#include + +/* ---- Forward declarations ---- */ + + +static void Initialize(Widget request, Widget new, + ArgList args, Cardinal *num_args); +static void Destroy(Widget w); +static void Redisplay(Widget w, XEvent *event, Region region); +static void Resize(Widget w); +static void InputAction(Widget w, XEvent *event, + String *params,Cardinal *num_params); +static Boolean SetValues(Widget current, Widget request, Widget new, + ArgList args, Cardinal *num_args); + + + + +/* ---- Private data ---- */ + + +static XtResource resources[] = { +#define offset(field) XtOffset(GraphWidget, graph.field) +#define coffset(field) XtOffset(Widget, core.field) + /* {name, class, type, size, offset, default_type, default_addr}, */ + { XtNcallback, XtCCallback, XtRCallback, (Cardinal)sizeof(XtCallbackList), + offset(input_callback), XtRCallback, NULL }, + { XtNdimBackground, XtCBackground, XtRPixel, (Cardinal)sizeof(Pixel), + offset(dimBackground), XtRString, "XtDefaultBackground" }, + { XtNexposeCallback, XtCCallback, XtRCallback, + (Cardinal)sizeof(XtCallbackList), + offset(expose_callback), XtRCallback, NULL }, + { XtNfont, XtCFont, XtRFontStruct, (Cardinal)sizeof(XFontStruct*), + offset(font), XtRString, XtDefaultFont }, + { XtNgraphColour1, XtCForeground, XtRPixel, (Cardinal)sizeof(Pixel), + offset(graphColour1), XtRString, "XtDefaultForeground" }, + { XtNgraphColour2, XtCForeground, XtRPixel, (Cardinal)sizeof(Pixel), + offset(graphColour2), XtRString, "XtDefaultForeground" }, + { XtNgraphColour3, XtCForeground, XtRPixel, (Cardinal)sizeof(Pixel), + offset(graphColour3), XtRString, "XtDefaultForeground" }, + { XtNgraphColour4, XtCForeground, XtRPixel, (Cardinal)sizeof(Pixel), + offset(graphColour4), XtRString, "XtDefaultForeground" }, + { XtNheight, XtCHeight, XtRDimension, (Cardinal)sizeof(Dimension), + coffset(height), XtRImmediate, (caddr_t)1}, + { XtNwidth, XtCWidth, XtRDimension, (Cardinal)sizeof(Dimension), + coffset(width), XtRImmediate, (caddr_t)1}, + { XtNresizeCallback, XtCCallback, XtRCallback, + (Cardinal)sizeof(XtCallbackList), + offset(resize_callback), XtRCallback, NULL }, +#undef offset +#undef coffset +}; + + +static XtActionsRec actions[] = +{ + /* {name, procedure}, */ + {"input", InputAction}, +}; + + +static char translations[] = +": input() \n\ + : input() \ +"; + + +GraphClassRec graphClassRec = { + { /* core fields initial values */ + /* superclass */ (WidgetClass) &simpleClassRec, + /* class_name */ "Graph", + /* widget_size */ (Cardinal)sizeof(GraphRec), + /* class_initialize */ NULL, + /* class_part_initialize */ NULL, + /* class_inited */ FALSE, + /* initialize */ Initialize, + /* initialize_hook */ NULL, + /* realize */ XtInheritRealize, + /* actions */ actions, + /* num_actions */ XtNumber(actions), + /* resources */ resources, + /* num_resources */ XtNumber(resources), + /* xrm_class */ NULLQUARK, + /* compress_motion */ TRUE, + /* compress_exposure */ XtExposeCompressMaximal, + /* compress_enterleave */ TRUE, + /* visible_interest */ FALSE, + /* destroy */ Destroy, + /* resize */ Resize, + /* expose */ Redisplay, + /* set_values */ SetValues, + /* set_values_hook */ NULL, + /* set_values_almost */ XtInheritSetValuesAlmost, + /* get_values_hook */ NULL, + /* accept_focus */ NULL, + /* version */ XtVersion, + /* callback_private */ NULL, + /* tm_table */ translations, + /* query_geometry */ XtInheritQueryGeometry, + /* display_accelerator */ XtInheritDisplayAccelerator, + /* extension */ NULL + }, + { /* Simple class initial values */ + /* change_sensitive */ XtInheritChangeSensitive + }, + { /* Graph class initial values */ + /* empty */ 0 + } +}; + +WidgetClass graphWidgetClass = (WidgetClass)&graphClassRec; + + + + +/* ---- Private procedures ---- */ + + +static void Initialize(Widget request, Widget new, + ArgList args, Cardinal *num_args) +{ GraphWidget gw = (GraphWidget)new; + unsigned long xGCMask; + XGCValues xGCValues; + Display *display; + int screen; + + display = XtDisplay(request); + screen = DefaultScreen(display); + + if (DisplayPlanes(display,screen)==1) + { /* We are on a one-plane monochrome display. + Use dashes to make lines distinguishable. + */ + xGCMask = GCForeground | GCBackground | GCLineStyle; + xGCValues.foreground = BlackPixel(display,screen); + xGCValues.background = WhitePixel(display,screen); + xGCValues.line_style = LineSolid; + gw->graph.graphGC1 = XtGetGC((Widget) gw, xGCMask, &xGCValues); + + xGCMask = GCForeground | GCBackground | GCLineStyle | GCDashList; + xGCValues.foreground = BlackPixel(display,screen); + xGCValues.background = WhitePixel(display,screen); + xGCValues.line_style = LineOnOffDash; + xGCValues.dashes = 1; + gw->graph.graphGC2 = XtGetGC((Widget) gw, xGCMask, &xGCValues); + + xGCMask = GCForeground | GCBackground | GCLineStyle | GCDashList; + xGCValues.foreground = BlackPixel(display,screen); + xGCValues.background = WhitePixel(display,screen); + xGCValues.line_style = LineOnOffDash; + xGCValues.dashes = 2; + gw->graph.graphGC3 = XtGetGC((Widget) gw, xGCMask, &xGCValues); + + xGCMask = GCForeground | GCBackground | GCLineStyle | GCDashList; + xGCValues.foreground = BlackPixel(display,screen); + xGCValues.background = WhitePixel(display,screen); + xGCValues.line_style = LineOnOffDash; + xGCValues.dashes = 4; + gw->graph.graphGC4 = XtGetGC((Widget) gw, xGCMask, &xGCValues); + + gw->graph.graphColour1 = BlackPixel(display,screen); + gw->graph.graphColour2 = BlackPixel(display,screen); + gw->graph.graphColour3 = BlackPixel(display,screen); + gw->graph.graphColour4 = BlackPixel(display,screen); + } + else + { /* Some sort of multi-plane display. + */ + xGCMask = GCForeground | GCBackground; + xGCValues.background = gw->core.background_pixel; + + xGCValues.foreground = gw->graph.graphColour1; + gw->graph.graphGC1 = XtGetGC((Widget) gw, xGCMask, &xGCValues); + + xGCValues.foreground = gw->graph.graphColour2; + gw->graph.graphGC2 = XtGetGC((Widget) gw, xGCMask, &xGCValues); + + xGCValues.foreground = gw->graph.graphColour3; + gw->graph.graphGC3 = XtGetGC((Widget) gw, xGCMask, &xGCValues); + + xGCValues.foreground = gw->graph.graphColour4; + gw->graph.graphGC4 = XtGetGC((Widget) gw, xGCMask, &xGCValues); + } +} + + +static void Destroy (Widget w) +{ GraphWidget gw = (GraphWidget) w; + + XtDestroyGC(gw->graph.graphGC1); + XtDestroyGC(gw->graph.graphGC2); + XtDestroyGC(gw->graph.graphGC3); + XtDestroyGC(gw->graph.graphGC4); +} + + +static void Redisplay(Widget w, XEvent *event, Region region) +{ XtCallCallbacks(w, XtNexposeCallback, (caddr_t)region); +} + + +static void Resize(Widget w) +{ XtCallCallbacks(w, XtNresizeCallback, 0); +} + + +static void InputAction(Widget w, XEvent *event, + String *params,Cardinal *num_params) +{ XtCallCallbacks(w, XtNcallback, (caddr_t)event); +} + + +static Boolean SetValues(Widget current, Widget request, Widget new, + ArgList args, Cardinal *num_args) +{ GraphWidget g_old = (GraphWidget) current; + GraphWidget g_new = (GraphWidget) new; + + if (DisplayPlanes(XtDisplay(new), DefaultScreen(XtDisplay(new))) == 1 || + !XtIsRealized(new)) + { /* Silly to change, or can't change. + Don't generate an expose. + */ + return(False); + } + else + { if (g_old->graph.graphColour1 != g_new->graph.graphColour1) + XSetForeground(XtDisplay(new), + g_new->graph.graphGC1, + g_new->graph.graphColour1); + + if (g_old->graph.graphColour2 != g_new->graph.graphColour2) + XSetForeground(XtDisplay(new), + g_new->graph.graphGC2, + g_new->graph.graphColour2); + + if (g_old->graph.graphColour3 != g_new->graph.graphColour3) + XSetForeground(XtDisplay(new), + g_new->graph.graphGC3, + g_new->graph.graphColour3); + + if (g_old->graph.graphColour4 != g_new->graph.graphColour4) + XSetForeground(XtDisplay(new), + g_new->graph.graphGC4, + g_new->graph.graphColour4); + + return(True); + } +} + + + + +/* ---- Exported procedures ---- */ + + +GC GraphGC1(Widget w) +{ return((GraphWidget)w)->graph.graphGC1; +} + + +GC GraphGC2(Widget w) +{ return((GraphWidget)w)->graph.graphGC2; +} + + +GC GraphGC3(Widget w) +{ return((GraphWidget)w)->graph.graphGC3; +} + + +GC GraphGC4(Widget w) +{ return((GraphWidget)w)->graph.graphGC4; +} diff --git a/src/ted/Graph.h b/src/ted/Graph.h new file mode 100644 index 0000000..99fe869 --- /dev/null +++ b/src/ted/Graph.h @@ -0,0 +1,81 @@ +#include + +/* $XConsortium: Graph.h,v 1.2 88/10/25 17:22:09 swick Exp $ */ +/* Copyright Massachusetts Institute of Technology 1987, 1988 */ + +#ifndef _Graph_h +#define _Graph_h + +/**************************************************************** + * + * Graph widget + * + ****************************************************************/ + +#include + +/* Resources: + + Name Class RepType Default Value + ---- ----- ------- ------------- + background Background Pixel XtDefaultBackground + border BorderColor Pixel XtDefaultForeground + borderWidth BorderWidth Dimension 1 + callback Callback Callback NULL + cursor Cursor Cursor None + destroyCallback Callback Pointer NULL + dimBackground Background Pixel XtDefaultBackground + exposeCallback Callback Callback NULL + font Font XFontStruct* XtDefaultFont + graphColour1 Foreground Pixel XtDefaultForeground + graphColour2 Foreground Pixel XtDefaultForeground + graphColour3 Foreground Pixel XtDefaultForeground + graphColour4 Foreground Pixel XtDefaultForeground + height Height Dimension 1 + insensitiveBorder Insensitive Pixmap Gray + mappedWhenManaged MappedWhenManaged Boolean True + resizeCallback Callback Callback NULL + sensitive Sensitive Boolean True + width Width Dimension 1 + x Position Position 0 + y Position Position 0 + +*/ + +/* define any special resource names here that are not in */ + +#define XtNgraphResource "graphResource" +#define XtCGraphResource "GraphResource" + +#define XtNdimBackground "dimBackground" +#define XtNexposeCallback "exposeCallback" +#define XtNgraphColour1 "graphColour1" +#define XtNgraphColour2 "graphColour2" +#define XtNgraphColour3 "graphColour3" +#define XtNgraphColour4 "graphColour4" +#define XtNresizeCallback "resizeCallback" + + +/* declare specific GraphWidget class and instance datatypes */ + +typedef struct _GraphClassRec *GraphWidgetClass; +typedef struct _GraphRec *GraphWidget; + + +/* declare the class constant */ + +extern WidgetClass graphWidgetClass; + + +/* ---- Exported procedures ---- */ + +extern Pixel GraphColour1(Widget w); +extern Pixel GraphColour2(Widget w); +extern Pixel GraphColour3(Widget w); +extern Pixel GraphColour4(Widget w); +extern GC GraphGC1(Widget w); +extern GC GraphGC2(Widget w); +extern GC GraphGC3(Widget w); +extern GC GraphGC4(Widget w); + +#endif /* _Graph_h */ diff --git a/src/ted/GraphP.h b/src/ted/GraphP.h new file mode 100644 index 0000000..07c673f --- /dev/null +++ b/src/ted/GraphP.h @@ -0,0 +1,54 @@ +#include + +/* $XConsortium: GraphP.h,v 1.2 88/10/25 17:37:59 swick Exp $ */ +/* Copyright Massachusetts Institute of Technology 1987, 1988 */ + +#ifndef _GraphP_h +#define _GraphP_h + +#include "Graph.h" +/* include superclass private header file */ +#include + + +/* define unique representation types not found in */ + +#define XtRGraphResource "GraphResource" + +typedef struct { + int empty; +} GraphClassPart; + +typedef struct _GraphClassRec { + CoreClassPart core_class; + SimpleClassPart simple_class; + GraphClassPart graph_class; +} GraphClassRec; + +extern GraphClassRec graphClassRec; + +typedef struct { + /* resources */ + Pixel dimBackground; + XFontStruct* font; + Pixel graphColour1; + Pixel graphColour2; + Pixel graphColour3; + Pixel graphColour4; + XtCallbackList expose_callback; + XtCallbackList resize_callback; + XtCallbackList input_callback; + /* private state */ + GC graphGC1; + GC graphGC2; + GC graphGC3; + GC graphGC4; +} GraphPart; + +typedef struct _GraphRec { + CorePart core; + SimplePart simple; + GraphPart graph; +} GraphRec; + +#endif /* _GraphP_h */ diff --git a/src/ted/README b/src/ted/README new file mode 100644 index 0000000..670da50 --- /dev/null +++ b/src/ted/README @@ -0,0 +1,32 @@ +ted - trace editor +------------------ +This is the source directory for the ted trace editor. +As well as the sources you should find a makefile, +a manual page "ted.manpage", documentation "ted.tex" +(this preprint is still in review and should be treated +as confidential until it has been accepted for publication) +a help file "ted.help" and a default resources file "Xted". + +The "ted.help" and "Xted" files should be placed in the +"/usr/lib/X11/app-defaults" directory. The "ted.manpage" +file should be placed in "/usr/man/manl/ted.l", or wherever +local manual pages are stored on your system. + +The "ted" application can be constructed by simply typing +"make ted". The "ted" application can then be placed in +a generally accessible directory of binaries, perhaps +"/usr/local/bin". + + + +sample files +------------ + +A sample ABI file is also included in this directory, trace.abi, which + can be opened using the following command: + ted -ABI trace.abi + +A sample ALF file is also included in this directory, trace.alf, which + can be opened using the following command: + ted -ALF trace.alf + diff --git a/src/ted/Xted b/src/ted/Xted new file mode 100644 index 0000000..d01cbb9 --- /dev/null +++ b/src/ted/Xted @@ -0,0 +1,178 @@ +! +! Resources for the "ted" trace editor (Dec 1990) +! + +! default magnification +Xted*magnif: 30 +! default enzyme string to search for as a left cutoff +Xted*enz: CAGGTC + +! The application +Xted.minWidth: 550 +Xted.minHeight: 250 + +! Labels have no borders and are not resized +Xted*Label.borderWidth: 0 +Xted*Label.top: chainTop +Xted*Label.bottom: chainTop +Xted*Label.left: chainLeft +Xted*Label.right: chainLeft + +! Buttons are not resized +Xted*Command.top: chainTop +Xted*Command.bottom: chainTop +Xted*Command.left: chainLeft +Xted*Command.right: chainLeft + +! All the toggles are one-of-many +Xted*Toggle.Translations: #override \n\ + : highlight(Always) \n\ + : unhighlight() \n\ + ,: set() notify() + +Xted.mainForm.title.label: Trace editor +Xted.mainForm.version.label: \ +Dec 1990 +Xted.mainForm.seqNamePrompt.label: Input: +Xted.mainForm.seqNamePrompt.height: 13 +Xted.mainForm.seqName.label: NONE +Xted.mainForm.seqName.resizable: True +Xted.mainForm.seqName.height: 13 +Xted.mainForm.search.label: Search +Xted.mainForm.search.height: 13 +Xted.mainForm.NorigBasesPrompt.label: Bases: +Xted.mainForm.NorigBases.label: +Xted.mainForm.NorigBases.resizable: True +Xted.mainForm.strand.label: Strand: Top +Xted.mainForm.strand.resizable: True + +! Display forms have no border +Xted.mainForm.Form.borderWidth: 0 + + ! The edit mode toggles +Xted.mainForm.Toggle.top: chainTop +Xted.mainForm.Toggle.bottom: chainTop +Xted.mainForm.Toggle.left: chainLeft +Xted.mainForm.Toggle.right: chainLeft +Xted.mainForm.edmodelab.label: Mode: +Xted.mainForm.adjustL.label: Adj left cut +Xted.mainForm.edSeq.label: Edit seq +Xted.mainForm.adjustR.label: Adj right cut +Xted.mainForm.edSeq.state: True +Xted.mainForm.adjustL.height: 13 +Xted.mainForm.edSeq.height: 13 +Xted.mainForm.adjustR.height: 13 + + +! The magnification slider +Xted.mainForm.maglab.top: chainTop +Xted.mainForm.maglab.bottom: chainTop +Xted.mainForm.maglab.left: chainLeft +Xted.mainForm.maglab.right: chainLeft +Xted.mainForm.maglab.label: Mag: +Xted.mainForm.maglab.height: 13 +Xted.mainForm.magscr.top: chainTop +Xted.mainForm.magscr.bottom: chainTop +Xted.mainForm.magscr.left: chainLeft +Xted.mainForm.magscr.right: chainLeft +Xted.mainForm.magscr.orientation: horizontal +Xted.mainForm.magscr.length: 100 +Xted.mainForm.magscr.height: 13 + +Xted.mainForm.scaleDown.label: Scale down +Xted.mainForm.scaleDown.height: 13 +Xted.mainForm.scaleUp.label: Scale up +Xted.mainForm.scaleUp.height: 13 +Xted.mainForm.switch.label: Switch +Xted.mainForm.switch.height: 13 + +! The main display: a viewport containing a form containing graphs +Xted.mainForm.viewport.top: chainTop +Xted.mainForm.viewport.bottom: chainBottom +Xted.mainForm.viewport.left: chainLeft +Xted.mainForm.viewport.right: chainRight +Xted.mainForm.viewport.allowHoriz: True +Xted.mainForm.viewport.forcebars: True +Xted.mainForm.viewport.width: 700 +Xted.mainForm.viewport.vpForm.Graph.resizable: True +Xted.mainForm.viewport.vpForm.Graph.top: chainTop +Xted.mainForm.viewport.vpForm.Graph.bottom: chainTop +Xted.mainForm.viewport.vpForm.Graph.left: chainLeft +Xted.mainForm.viewport.vpForm.Graph.right: chainRight +Xted.mainForm.viewport.vpForm.trace.top: chainTop +Xted.mainForm.viewport.vpForm.trace.bottom: chainBottom +Xted.mainForm.viewport.vpForm.Graph.font: -misc-*-bold-*15-* +! The text graph heights are dynamically set to accomodate the font +Xted.mainForm.viewport.vpForm.trace.height: 150 +! The graphs are dynamically initially set to fill the viewport +Xted.mainForm.viewport.vpForm.Graph.dimBackground: grey +Xted.mainForm.viewport.vpForm.Graph.graphColour1: green +Xted.mainForm.viewport.vpForm.Graph.graphColour2: purple +Xted.mainForm.viewport.vpForm.Graph.graphColour3: black +Xted.mainForm.viewport.vpForm.Graph.graphColour4: red + +! Dummy widget - we only care about its width +Xted.mainForm.dummy.top: chainTop +Xted.mainForm.dummy.bottom: chainBottom +Xted.mainForm.dummy.left: chainLeft +Xted.mainForm.dummy.right: chainRight +Xted.mainForm.dummy.width: 700 + + +! Dialogues +Xted*search.ioForm.ioPrompt.label: Search string or base number +Xted*input.ioForm.ioPrompt.label: Input a file +Xted*output.ioForm.ioPrompt.label: Save a file +Xted*check.ioForm.ioPrompt.label: \ +The existing sequence has been altered.\n\ +Do you really want to overwrite it? +Xted*quit.ioForm.ioPrompt.label: \ +The existing sequence has been altered.\n\ +Do you really want to quit? +Xted*check.ioForm..translations: #override \n\ + Return: CRAction() +Xted*quit.ioForm..translations: #override \n\ + Return: CRAction() + +Xted*ioForm.namePrompt.label: File name: +Xted*ioForm.nameVal.width: 250 +Xted*ioForm.nameVal.translations: #override \n\ + Return: CRAction() +Xted*ioForm.next.label: +Xted*ioForm.next.resizable: True +Xted*ioForm.formatPrompt.label: File format: +Xted*ioForm.formatBox.borderWidth: 0 +Xted*ioForm.formatBox.orientation: horizontal +Xted*ioForm.formatBox.plainFmt.label: Plain +Xted*ioForm.formatBox.abiFmt.label: ABI +Xted*ioForm.formatBox.abiFmt.state: True +Xted*ioForm.formatBox.alfFmt.label: ALF +Xted*ioForm.formatBox.alfFmt.state: False +Xted*ioForm.formatBox.scfFmt.label: SCF +Xted*ioForm.formatBox.scfFmt.state: False +Xted*ioForm.formatBox.bottomFmt.label: Bottom +Xted*ioForm.formatBox.topFmt.label: Top +Xted*ioForm.formatBox.topFmt.state: True + +! The `default' button for the dialogue has a wider border +Xted*input.ioForm.OK.borderWidth: 3 +Xted*output.ioForm.OK.borderWidth: 3 +Xted*check.ioForm.Cancel.borderWidth: 3 +Xted*quit.ioForm.Cancel.borderWidth: 3 + +! Help shell widget +Xted.Help.width: 525 +Xted.Help.height: 200 +Xted.Help.minWidth: 525 +Xted.Help.maxWidth: 525 +Xted.Help.minHeight: 150 +Xted.Help.title: ted help +Xted.Help.form.rmButton.label: Remove help +Xted.Help.form.rmButton.top: chainTop +Xted.Help.form.rmButton.bottom: chainTop +Xted.Help.form.rmButton.left: chainLeft +Xted.Help.form.rmButton.right: chainLeft +Xted.Help.form.text.top: chainTop +Xted.Help.form.text.bottom: chainBottom +Xted.Help.form.text.left: chainLeft +Xted.Help.form.text.right: chainRight diff --git a/src/ted/basesDisplay.c b/src/ted/basesDisplay.c new file mode 100644 index 0000000..79ada06 --- /dev/null +++ b/src/ted/basesDisplay.c @@ -0,0 +1,1014 @@ +/* + Title: basesDisplay + + File: basesDisplay.h + Purpose: Display the bases of a sequence on graphs + Last update: Wednesday 8 April 1992 +*/ + + +/* + `plotEdgeOffset' indicates the pixel position at which point 0 + occurs. The characters for bases are printed with their centres + corresponding to their locations on the plot. + + A caret is XOR drawn in the editable graph on top of (i.e. after) + all characters have been drawn. + + Changes to this module by lfw: + module displayEdBases and displayBases + added int baseNum as a parameter so that + the user could specify a baseNum via the + command line baseNum parameter or via + specifying a string to search for ( if + you specify a string to search for + ) and have that be where the caret + is originally positioned. Before this + modification, the caret was always + initially positioned at base 0. + Changes made by sd: + leftCutoffPoint: handle NULLPoint + Changes made by SD: + Support monochrome displays when marking cutoffs + +*/ + + + + +/* ---- Includes ---- */ + +#include "basesDisplay.h" +#include "display.h" + +#include "Graph.h" +#include "seq.h" /* IMPORT: Seq, NULLSeq, getNPoints */ + +#include /* IMPORT: toupper */ + +#include +#include +#include +#include +#include +#include +#include +#include +#include + + + + +/* ---- Constants ---- */ + +/* Caret stuff */ +#define caretWidth ((Dimension) 6) +#define caretHeight ((Dimension) 3) +static char caretBits[] = {0x0c, 0x1e, 0x33}; + + + + +/* ---- Statics ---- */ + +/* Current sequence information */ +static Seq currSeq = NULLSeq; +static int NPoints; +static int NorigBases, NedBases; + + +/* Font information */ +static Dimension charWidth; +static Dimension halfCharWidth; +static Dimension charVOffset; /* Of char baseline from top of graph=ascent+1 */ + +/* The two graphs */ +static Widget origSeqWid; +static Widget edSeqWid; +/* the numbers above the two graphs*/ +static Widget baseNumWid; + +static Dimension graphWidth; /* Current width of the graph */ +static Dimension graphHeight; /* Height of the graph */ +static Dimension plotEdgeOffset; +static Dimension plotWidth; /* Current width of the plot */ + +static int leftCutoff, rightCutoff; + +static GC Agc, Cgc, Ggc, Tgc; +static Pixel normBackground, dimBackground; + +/* monochrome stippling */ +static GC greygc; +static int planes; + +/* Caret stuff */ +/* + The caret's position is represented by a base number, caretBN, (the + point at which the corresponding base occurs is maintained as caretP) + and is drawn to the right of that base. To allow insertion at the left + end and deletion at the right end, it can range from -1..NBase-1. +*/ +static GC caretGC; +static Pixmap caretPixmap = (Pixmap) 0; +static int caretBN; +static int caretP; + + + + + +/* ---- Position translation ---- */ + +/* + Convert a value from the `point-in-the-sequence' to the + `x-pixel-on-the-graph' scale, and vice-versa, given that + `plotWidth' holds the current pixel width of the graph which + starts `plotEdgeOffset' from either edge of the graph and + `NPoints' gives the current point width. + Fractional results are truncated - for pixelToPoint this may + mean a point one too low is indicated, but a point too high is + never indicated. +*/ + + +static int pointToPixel(int point) +{ return(((point * plotWidth) / (NPoints-1)) + plotEdgeOffset); +} + + +int pixelToPoint(int pixel) +{ return(((pixel-plotEdgeOffset) * (NPoints-1)) / plotWidth); +} + + +static int leftCutoffPoint(int baseNum) +/* + Return the point corresponding to a left cutoff at `baseNum'. +*/ +{ int pL, pR; + + pL = (baseNum == 0) ? 0 : baseNumToPoint(currSeq, EdBases, baseNum-1); + pR = (baseNum == 0) ? 0 : baseNumToPoint(currSeq, EdBases, baseNum); + + return((pR==NULLPoint)?pL:(pL+pR)/2); +} + + +static int rightCutoffPoint(int baseNum) +/* + Return the point corresponding to a right cutoff at `baseNum'. +*/ +{ int NedBases = getNBases(currSeq, EdBases); + int pL, pR; + + pL = (baseNum == 0) + ? NPoints-1 + : baseNumToPoint(currSeq, EdBases, NedBases-baseNum); + pR = (baseNum == 0) + ? NPoints-1 + : baseNumToPoint(currSeq, EdBases, NedBases-baseNum-1); + + return((pL+pR)/2); +} + + + + +/* ---- Internal routines ---- */ + + +static void writeBase(Widget wid, int baseNum, Widget bnwid) +{ WhichBases which = (wid == origSeqWid) ? OrigBases : EdBases; + char base = getBase(currSeq, which, baseNum); + int point = baseNumToPoint(currSeq, which, baseNum); + GC gc; + char lbl[10]; + + /* Get the correct GC */ + switch (base) + { case 'A': gc=Agc; break; + case 'C': gc=Cgc; break; + case 'G': gc=Ggc; break; + case 'T': gc=Tgc; break; + default: gc=Ggc; break; + } + + XDrawImageString(XtDisplay(wid), + XtWindow(wid), + gc, + pointToPixel(point)-halfCharWidth, charVOffset, + &base, 1); +/* if (currSeq->bottom) + sprintf(lbl,"%d",(currSeq->NorigBases-1)-baseNum); + else*/ + sprintf(lbl,"%d",baseNum); + if ((wid == origSeqWid) && (baseNum%10 == 0)) { + XDrawString(XtDisplay(bnwid), + XtWindow(bnwid), + gc, + pointToPixel(point)-halfCharWidth, charVOffset, + lbl,strlen(lbl)); + } +} + + + + +static void caretExpose(int *pix0, /* First exposed pixel */ + int *pixN) /* Last exposed pixel */ +/* + Extend the expose region, if necessary, so that the caret + is either completely, or not at all exposed. +*/ +{ int caretLwb, caretUpb; + + caretLwb = pointToPixel(caretP)+halfCharWidth; + caretUpb = caretLwb + caretWidth; + + /* Return if the caret is fully outside the exposed region */ + if ((caretUpb<*pix0) || (caretLwb>*pixN)) return; + + /* Return if the caret is fully inside the exposed region */ + if ((caretLwb>=*pix0) && (caretUpb<=*pixN)) return; + + /* Extend the exposed region */ + *pix0 = caretLwb<*pix0 ? caretLwb : *pix0; + *pixN = caretUpb>*pixN ? caretUpb : *pixN; + XClearArea(XtDisplay(edSeqWid), + XtWindow(edSeqWid), + caretLwb, 0, + caretWidth, graphHeight, + False); +} + + + + +static void drawCaret() +{ int caretLwb; + + caretLwb = pointToPixel(caretP)+halfCharWidth; + + XCopyPlane(XtDisplay(edSeqWid), + caretPixmap, XtWindow(edSeqWid), + caretGC, + 0, 0, + (unsigned int) caretWidth, (unsigned int) caretHeight, + caretLwb, charVOffset, + 1); +} + + + + +static void drawExposedCaret(int p0, /* First exposed pixel */ + int pN) /* Last exposed pixel */ +/* + Draw the caret, if it is within the exposed points. +*/ +{ int caretLwb; + + caretLwb = pointToPixel(caretP)+halfCharWidth; + + if ((caretLwb<=pN) && (caretLwb>=p0)) + drawCaret(); + else + return; +} + + + + +/* ---- Callbacks ---- */ + + +static void exposeCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ Region region = (Region) call_data; + XRectangle rect; + int x0, xN; /* Affected region: pixels */ + int p0, pN; /* Affected region: points */ + int b, b0, bN; /* Affected region: bases */ + int leftCutoffP, rightCutoffP; + int NBases; + int whichBases; + + if (currSeq == NULLSeq) return; + + getCutoffs(currSeq, &leftCutoff, &rightCutoff); + leftCutoffP = leftCutoffPoint(leftCutoff); + rightCutoffP = rightCutoffPoint(rightCutoff); + NBases = (wid==origSeqWid) ? NorigBases : NedBases; + whichBases = (wid==origSeqWid) ? OrigBases : EdBases; + + + /* + `region' gives us the exposed graph region. + XClipBox will return a bounding rectangle, in pixels. + */ + XClipBox(region, &rect); + x0 = rect.x; + xN = rect.x+rect.width; + + + /* For the editable sequence, ensure the caret was not half exposed */ + if (wid == edSeqWid) caretExpose(&x0, &xN); + + + /* Convert to affected points after trimming to the plot proper */ + p0=pixelToPoint((x0plotWidth+plotEdgeOffset)?plotWidth+plotEdgeOffset:xN); + if (pNrightCutoffP) + /* + Some of the exposed region is in the right cutoff area. + Repaint it with dimmed background. + */ + { int firstP = (p0>rightCutoffP) ? p0 : rightCutoffP; + int lastP = pN; + + if (whichBases == EdBases) + { int pix0 = pointToPixel(firstP); + int pixN = pointToPixel(lastP); + + if (planes == 1) { + XFillRectangle(XtDisplay(edSeqWid), XtWindow(edSeqWid), + greygc, + pix0, 0, + pixN-pix0, graphHeight); + } else { + XSetWindowBackground(XtDisplay(edSeqWid), XtWindow(edSeqWid), + dimBackground); + XClearArea(XtDisplay(edSeqWid), XtWindow(edSeqWid), + pix0, 0, + pixN-pix0, graphHeight, + False); + XSetWindowBackground(XtDisplay(edSeqWid), XtWindow(edSeqWid), + normBackground); + } + } + } + + + /* + Now draw the affected bases. + */ + + /* The first base is to the left of the exposed region */ + b0 = pointToBaseNum(currSeq, whichBases, p0); + b0 = (b0 == NULLBaseNum) ? NBases-1 + : (b0>0) ? b0-1 + : 0; + /* The last base is to the right of the exposed region */ + bN = pointToBaseNum(currSeq, whichBases, pN); + bN = (bN == NULLBaseNum) ? NBases-1 : bN; + + if (b0=leftCutoff && b0-NedBases-1NedBases-1-rightCutoff) + ? NedBases-1-rightCutoff + : bN; + + for (b=firstBase; b<=lastBase; b++) writeBase(wid, b, baseNumWid); + } + + if (bN>NedBases-1-rightCutoff) + /* + Some of the exposed region is in the right cutoff area. + Repaint it with dimmed background. + */ + { if (whichBases == EdBases) + { XSetBackground(XtDisplay(wid), Agc, dimBackground); + XSetBackground(XtDisplay(wid), Cgc, dimBackground); + XSetBackground(XtDisplay(wid), Ggc, dimBackground); + XSetBackground(XtDisplay(wid), Tgc, dimBackground); + } + + /* Plot the affected bases */ + for (b=NedBases-rightCutoff; b<=bN; b++) writeBase(wid, b, baseNumWid); + + if (whichBases == EdBases) + { XSetBackground(XtDisplay(wid), Agc, normBackground); + XSetBackground(XtDisplay(wid), Cgc, normBackground); + XSetBackground(XtDisplay(wid), Ggc, normBackground); + XSetBackground(XtDisplay(wid), Tgc, normBackground); + } + } + + + /* If this is the editable sequence, display the caret */ + if (wid == edSeqWid) drawExposedCaret(x0, xN); +} + + + + +static void userCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +/* + Handles all XtNcallbacks, i.e., buttons and keys. +*/ +{ XEvent *eventP = (XEvent *) call_data; + + if (currSeq == NULLSeq) return; + + /* + Callback to the `display' module. + */ + userEvent(eventP); +} + + + + +/* ---- Exports ---- */ +void redisplayBases() +{ + /* + ** Clear the area and generate an expose. + */ + XClearArea(XtDisplay(baseNumWid), XtWindow(baseNumWid), + 0, 0, + 0, 0, + True); + XClearArea(XtDisplay(origSeqWid), XtWindow(origSeqWid), + 0, 0, + 0, 0, + True); + XClearArea(XtDisplay(edSeqWid), XtWindow(edSeqWid), + 0, 0, + 0, 0, + True); +} + + + + +Widget createBasesDisplay(Widget parentWid, Widget fromVertWid, + Dimension width) +/* + Create the bases display within the Form widget `parent', + with the XtNfromVert constraint `fromVertWid'. The initial + width is `width'. Return the (lower) widget constructed. + No bases are initially displayed. +*/ +{ Arg args[10]; + int nargs; + + currSeq = NULLSeq; + graphWidth = width; + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNwidth, graphWidth); nargs++; + baseNumWid = XtCreateManagedWidget("baseNumWid", graphWidgetClass, + parentWid, args, nargs); + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNfromVert, baseNumWid); nargs++; + XtSetArg(args[nargs], XtNwidth, graphWidth); nargs++; + origSeqWid = XtCreateManagedWidget("origSeq", graphWidgetClass, + parentWid, args, nargs); + XtAddCallback(origSeqWid, XtNcallback, userCallback, NULL); + XtAddCallback(origSeqWid, XtNexposeCallback, exposeCallback, NULL); + + /* + Get font information. + The text graphs are set to be two pixels higher than + (ascent+descent). Characters are then plotted with + a vertical offset one more than the ascent. + */ + { XFontStruct *fontStruct; + + nargs = 0; + XtSetArg(args[nargs], XtNfont, &fontStruct); nargs++; + XtGetValues(origSeqWid, args, nargs); + charWidth = fontStruct->max_bounds.width; + halfCharWidth = fontStruct->max_bounds.width/2; + charVOffset = fontStruct->ascent+1; + plotEdgeOffset = halfCharWidth+2; + plotWidth = graphWidth-(2*plotEdgeOffset); + graphHeight = fontStruct->ascent + fontStruct->descent + 2; + /* Make this graph tall enough to hold it */ + nargs = 0; + XtSetArg(args[nargs], XtNheight, graphHeight); nargs++; + XtSetValues(origSeqWid, args, nargs); + /* Make this graph tall enough to hold it */ + nargs = 0; + XtSetArg(args[nargs], XtNheight, graphHeight); nargs++; + XtSetValues(baseNumWid, args, nargs); + } + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, origSeqWid); nargs++; + XtSetArg(args[nargs], XtNwidth, graphWidth); nargs++; + XtSetArg(args[nargs], XtNheight, graphHeight); nargs++; + edSeqWid = XtCreateManagedWidget("edSeq", graphWidgetClass, + parentWid, args, nargs); + XtAddCallback(edSeqWid, XtNcallback, userCallback, NULL); + XtAddCallback(edSeqWid, XtNexposeCallback, exposeCallback, NULL); + + + /* + Get the GCs + */ + Agc = GraphGC1(origSeqWid); + Cgc = GraphGC2(origSeqWid); + Ggc = GraphGC3(origSeqWid); + Tgc = GraphGC4(origSeqWid); + + /* + Get the backgrounds + */ + nargs = 0; + XtSetArg(args[nargs], XtNbackground, &normBackground); nargs++; + XtSetArg(args[nargs], XtNdimBackground, &dimBackground); nargs++; + XtGetValues(edSeqWid, args, nargs); + + planes = DisplayPlanes(XtDisplay(edSeqWid),DefaultScreen(XtDisplay(edSeqWid))); + if (planes == 1) { + /* prepare for monochrome display */ + XtGCMask valuemask = (GCStipple | GCFillStyle); + XGCValues values; + Pixmap grey_stipple; + +#define grey_width 4 +#define grey_height 4 + static char grey_bits[] = { + 0x0e, 0x0b, 0x0e, 0x0b}; + + grey_stipple = + XCreateBitmapFromData(XtDisplay(edSeqWid), + RootWindowOfScreen(XtScreenOfObject(edSeqWid)), + grey_bits, + grey_width, + grey_height); + + values.stipple = grey_stipple; + values.fill_style = FillOpaqueStippled; + greygc = XCreateGC(XtDisplayOfObject(edSeqWid), + RootWindowOfScreen(XtScreenOfObject(edSeqWid)), + valuemask, &values); + + } + + return(edSeqWid); +} + + + + +void getBasesFontInfo(Dimension *offset, Dimension *chWidth) +/* + In `offset' return the offset which the bases are drawn + from the left and right hand edges. The width of the characters + used is returned in `chWidth'. +*/ +{ *offset = plotEdgeOffset; + *chWidth = charWidth; +} + + + + +void displayBases(Seq seq, int baseNum) +/* + Display the bases of sequence `seq'. +*/ +{ + if (seq == NULLSeq) return; + currSeq = seq; + + + /* + Get static information about the current sequence. + */ + NPoints = getNPoints(currSeq); + NorigBases = getNBases(currSeq, OrigBases); + NedBases = getNBases(currSeq, EdBases); /* Should be the same */ + getCutoffs(currSeq, &leftCutoff, &rightCutoff); + + + /* + Ensure the caret for the editable graph exists. + This is *not* the place to do this, but it has to be done + when we are sure the graph has been realised. + In either case, move it to the start. + */ +/* if (caretPixmap == (Pixmap) 0)*/ + if (caretPixmap == (Pixmap) 0) + { XGCValues values; + + caretPixmap = XCreateBitmapFromData(XtDisplay(edSeqWid), + XtWindow(edSeqWid), + caretBits, + caretWidth, + caretHeight); + + values.function = GXxor; + caretGC = XCreateGC(XtDisplay(edSeqWid), XtWindow(edSeqWid), + GCFunction, &values); + XCopyGC(XtDisplay(edSeqWid), + GraphGC3(edSeqWid), + (GCForeground | GCBackground | GCLineStyle | GCDashList), + caretGC); + } + + /* + Initially, the caret is placed hard to the left. + + caretBN = -1; + caretP = 0; */ + +/* modification by lfw 10/24/90, + the following section added to move the caret to the position + the user requests for baseNum , either by specifying + -baseNum on the command line or -astring */ + + caretBN = baseNum-1; +/* baseNum-1 because it inserts the caret at the space after baseNum */ + + if (baseNum == -1) + caretP = 0; + else + caretP = baseNumToPoint(currSeq, EdBases, caretBN); + moveCaretTo(baseNum-1); + +} + + +void displayEdBases(Seq seq, int baseNum) +/* + Display the bases of sequence `seq'. +*/ +{ + if (seq == NULLSeq) return; + currSeq = seq; + + + /* + Get static information about the current sequence. + */ + NedBases = getNBases(currSeq, EdBases); + getCutoffs(currSeq, &leftCutoff, &rightCutoff); + + + /* + Ensure the caret for the editable graph exists. + This is *not* the place to do this, but it has to be done + when we are sure the graph has been realised. + In either case, move it to the start. + */ +/* if (caretPixmap == (Pixmap) 0)*/ + if (caretPixmap == (Pixmap) 0) + { XGCValues values; + + caretPixmap = XCreateBitmapFromData(XtDisplay(edSeqWid), + XtWindow(edSeqWid), + caretBits, + caretWidth, + caretHeight); + + values.function = GXxor; + caretGC = XCreateGC(XtDisplay(edSeqWid), XtWindow(edSeqWid), + GCFunction, &values); + XCopyGC(XtDisplay(edSeqWid), + GraphGC3(edSeqWid), + (GCForeground | GCBackground | GCLineStyle | GCDashList), + caretGC); + } + + /* + Initially, the caret is placed hard to the left. + + caretBN = -1; + caretP = 0; */ + +/* modification by lfw 10/24/90, + the following section added to move the caret to the position + the user requests for baseNum , either by specifying + -baseNum on the command line or -astring */ + + caretBN = baseNum-1; +/* baseNum-1 because it inserts the caret at the space after baseNum */ + + if (baseNum == -1) + caretP = 0; + else + caretP = baseNumToPoint(currSeq, EdBases, caretBN); + moveCaretTo(baseNum-1); + +} + + + + +void unDisplayBases() +/* + Cease displaying the current sequence (if any). +*/ +{ currSeq = NULLSeq; +} + + + + +void setBasesWidth(Dimension width) +/* + Set the width of the current sequence bases display. +*/ +{ Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNwidth, width); nargs++; + XtSetValues(origSeqWid, args, nargs); + XtSetValues(edSeqWid, args, nargs); + XtSetValues(baseNumWid, args, nargs); + + graphWidth = width; + plotWidth = graphWidth-(2*plotEdgeOffset); + + /* + Clear the graphs. + */ + if (XtIsRealized(origSeqWid)) + XClearWindow(XtDisplay(origSeqWid), XtWindow(origSeqWid)); + if (XtIsRealized(edSeqWid)) + XClearWindow(XtDisplay(edSeqWid), XtWindow(edSeqWid)); + if (XtIsRealized(baseNumWid)) + XClearWindow(XtDisplay(baseNumWid), XtWindow(baseNumWid)); +} + + + + +int getCaret() +/* + Get the current position of the caret. +*/ +{ return(caretBN); +} + + + + +void moveCaretLeft() +/* + Move the caret left one base. +*/ +{ drawCaret(); + + caretBN = (caretBN==-1) ? -1 : caretBN-1; + caretP = (caretBN==-1) ? 0 : baseNumToPoint(currSeq, EdBases, caretBN); + + drawCaret(); +} + + + + +void moveCaretRight() +/* + Move the caret right one base. +*/ +{ drawCaret(); + + caretBN = (caretBN==NedBases-1) ? NedBases-1 : caretBN+1; + caretP = baseNumToPoint(currSeq, EdBases, caretBN); + + drawCaret(); +} + + + + +void moveCaretTo(int baseNum) +/* + Move the caret to after base `baseNum'. +*/ +{ drawCaret(); + + caretBN = baseNum; + caretP = (caretBN==-1) ? 0 : baseNumToPoint(currSeq, EdBases, caretBN); + + drawCaret(); +} + + + + +void baseInserted() +/* + The editable sequence has changed by the insertion of a base + to the right of the base indicated by the caret. Update our display. +*/ +{ int point, pix; + + drawCaret(); + + NedBases++; + caretBN++; + caretP = baseNumToPoint(currSeq, EdBases, caretBN); + + drawCaret(); + + /* Expose the new base */ + point = baseNumToPoint(currSeq,EdBases,caretBN); + pix = pointToPixel(point) - halfCharWidth; + XClearArea(XtDisplay(edSeqWid), XtWindow(edSeqWid), + pix, 0, + charWidth, graphHeight, + True); +} + + + + +void baseDeleted() +/* + The editable sequence has changed by the deletion of a base + from the left of the caret. Update our display. +*/ +{ int basePix = pointToPixel(caretP) - halfCharWidth; + + drawCaret(); + + NedBases--; + caretBN--;; + caretP = (caretBN==-1) ? 0 : baseNumToPoint(currSeq, EdBases, caretBN); + + drawCaret(); + + /* Clear and expose the deleted base */ + XClearArea(XtDisplay(edSeqWid), XtWindow(edSeqWid), + basePix, 0, + charWidth, graphHeight, + True); +} + + + + +void basesCutoffChange() +/* + One or both of the cutoffs have changed. + Update our display. +*/ +{ int newLeftCutoff, newRightCutoff; + + getCutoffs(currSeq, &newLeftCutoff, &newRightCutoff); + + + if (newLeftCutoff != leftCutoff) + /* + The region between newLeftCutoff and leftCutoff has changed. + */ + { int b0, bN; + int p0, pN; + int pix0, pixN; + + /* + b0 = leftmost base number. bN = rightmost base number. + */ + if (newLeftCutoff /* IMPORT: Widget */ + + + + +/* ---- Exports ---- */ + + +extern Widget createBasesDisplay(Widget parentWid, Widget fromVertWid, + Dimension width); +/* + Create the bases display within the Form widget `parent', + with the XtNfromVert constraint `fromVertWid'. The initial + width is `width'. Return the (lower) widget constructed. + No bases are initially displayed. +*/ + + +extern void getBasesFontInfo(Dimension *offset, Dimension *chWidth); +/* + In `offset' return the offset which the bases are drawn + from the left and right hand edges. The width of the characters + used is returned in `chWidth'. +*/ + + +extern void displayBases(Seq seq, int baseNum); +/* + Display the bases of sequence `seq'. +*/ + + +extern void unDisplayBases(); +/* + Cease displaying the bases of the current sequence (if any). +*/ + + +extern void setBasesWidth(Dimension width); +/* + Set the width of the current sequence bases display. +*/ + + +extern int getCaret(); +/* + Get the current position of the caret. +*/ + + +extern void moveCaretLeft(); +/* + Move the caret left one base. +*/ + + +extern void moveCaretRight(); +/* + Move the caret right one base. +*/ + + +extern void moveCaretTo(int baseNum); +/* + Move the caret to after base `baseNum'. +*/ + + +extern void baseInserted(); +/* + The editable sequence has changed by the insertion of a base + to the right of the base indicated by the caret. Update our display. +*/ + + +extern void baseDeleted(); +/* + The editable sequence has changed by the deletion of a base + from the left of the caret. Update our display. +*/ + + +extern int pixelToPoint(int pixel); +/* + Given `pixel' on the bases display, return the corresponding point. + Fractional results are truncated - this may mean a point one too + low is indicated, but a point too high is never indicated. +*/ + + +extern void basesCutoffChange(); +/* + One or both of the cutoffs have changed. + Update our display. +*/ diff --git a/src/ted/dialogues.c b/src/ted/dialogues.c new file mode 100644 index 0000000..c8ec834 --- /dev/null +++ b/src/ted/dialogues.c @@ -0,0 +1,1307 @@ +/* + Title: dialogues + + File: dialogues.c + Purpose: Dialogues + Last update: Thurs 31 October 1991 + + Change log: + + 27.11.90 SD outputOKCallback() now passes seqName in writeSeqABI() call + 28.11.90 SD put undesirables under STLOUIS compilation flag + 02.01.91 SD remerged with St Louis version + 22.07.91 LFW put in -enzyme as a command line option for STLOUIS + 17.09.91 LFW changed MRC switch to !AUTO_CLIP + changed STLOUIS switch to OUT_SEQ + 31.10.91 SD Popup dialogue if file exists on output + 26.11.91 SD Added SCF format +*/ + + + + +/* ---- Includes ---- */ + + +#include "values.h" /* IMPORT: MAXLONG */ + +#include "dialogues.h" +#include "display.h" /* IMPORT: displaySeq, unDisplaySeq */ +#include "info.h" /* IMPORT: info */ +#include "seq.h" /* IMPORT: Seq, NULLSeq */ +#include "seqRead.h" /* IMPORT: readSeq */ +#include "seqIOEdit.h" /* IMPORT: isDotNum, stripDotNum */ +#include "seqOutput.h" /* IMPORT: writeSeq */ + +#include +#include +#include +#include +#include +#include +#include +#include +#include + + +/* ---- Globals ---- */ + +char o_fn[200]; +char r_fn[200]; + +/* ---- Statics ---- */ + +static Seq currSeq = NULLSeq; +static int indices[100]; /* array of starting indices of all of the + matches a search for a string found */ +static int num_matches; /* total number of matches to a given + string found in the currSeq */ +static int next_indices; /* the next indices to search for + if the user wishes to search for + the next occurence of string */ + +/* ----- get resources -------*/ +/* allows you to get the default enzString to search for + from the Xted file */ + +typedef struct +{ int magnif; + String enz; +} AppData, *AppDataPtr; + +#define XtNmagnif "magnif" +#define XtCMagnif "Magnif" +#define XtNenz "enz" +#define XtCEnz "Enz" + +static XtResource resources[] = { + { XtNmagnif, + XtCMagnif, + XtRInt, + sizeof(int), + XtOffset(AppDataPtr, magnif), + XtRImmediate, + NULL + }, + { XtNenz, + XtCEnz, + XtRString, + sizeof(String), + XtOffset(AppDataPtr, enz), + XtRImmediate, + NULL + } + }; + + + +void set_strand_label(Widget strandWid, Seq seq) +{ + Cardinal nargs; + Arg args[1]; + + nargs = 0; + if (seq->bottom) { + XtSetArg(args[nargs], XtNlabel, "Strand: Bottom"); nargs++; + } else { + XtSetArg(args[nargs], XtNlabel, "Strand: Top"); nargs++; + } + XtSetValues(strandWid, args, nargs); +} + + +/* ---- Callbacks ---- */ + + +static void inputOKCallback(Widget w, + XtPointer client_data, XtPointer call_data) +{ + Widget popupWid, nameValWid, radioGroupWid, radioGroupWid2; + Widget seqNameWid, NorigBasesWid,strandWid; + char *radioData, *radioData2, *fn; + Arg args[10]; + int nargs; + Seq oldSeq = currSeq; + int bottom; + char enzString[100]; + AppData app_data; + + + popupWid = (Widget) client_data; + nameValWid = XtNameToWidget(popupWid, "ioForm.nameVal"); + radioGroupWid = XtNameToWidget(popupWid, "ioForm.formatBox.plainFmt"); + radioGroupWid2 = XtNameToWidget(popupWid, "ioForm.formatBox.bottomFmt"); + seqNameWid = XtNameToWidget(XtParent(XtParent(popupWid)), "seqName"); + NorigBasesWid = XtNameToWidget(XtParent(XtParent(popupWid)), "NorigBases"); + strandWid = XtNameToWidget(XtParent(XtParent(popupWid)), "strand"); + + XtGetApplicationResources(popupWid, (XtPointer) &app_data, + resources, XtNumber(resources), + NULL, 0); + +#ifndef AUTO_CLIP + app_data.enz='\0'; +#endif + + /* LFW, 05.02.91, for command line option to work */ + if (strcmp(enzString,"")==0) { + if (app_data.enz != NULL) + strcpy(enzString,app_data.enz); + else *enzString = '\0'; + } + /* + Get the name and type of the new one and read it in. + */ + nargs = 0; + XtSetArg(args[nargs], XtNstring, &fn); nargs++; + XtGetValues(nameValWid, args, nargs); + + radioData = (char *) XawToggleGetCurrent(radioGroupWid); + radioData2 = (char *) XawToggleGetCurrent(radioGroupWid2); + bottom = (strcmp(radioData2, "bottomFmt") == 0); + currSeq = readSeq(fn,bottom,enzString,radioData); + +#ifdef DEF_OUT /* out_seq means default output file name + of input_filename.seq */ + /* make a default output filename */ + sprintf(o_fn,"%s",fn); + if (isDotNum(o_fn) != -1) stripDotNum(o_fn); +#endif + + if (currSeq != NULLSeq) + { char NBasesString[10]; + + XtDestroyWidget(popupWid); + + /* + Undisplay and dispose of the old sequence. + */ + unDisplaySeq(); + freeSeq(oldSeq); + + /* + Set the `file name' and `number of bases' labels. + */ + nargs = 0; + XtSetArg(args[nargs], XtNlabel, fn); nargs++; + XtSetValues(seqNameWid, args, nargs); + nargs = 0; + sprintf(NBasesString, "%d", getNBases(currSeq, OrigBases)); + XtSetArg(args[nargs], XtNlabel, NBasesString); nargs++; + XtSetValues(NorigBasesWid, args, nargs); + /* set strand label */ + set_strand_label(strandWid, currSeq); + + /* original line displaySeq(currSeq, -1, 1.0);, + new line added by lfw: */ + displaySeq(currSeq, 1, 0.3); + } + else + { XBell(XtDisplay(popupWid), 100); + nargs = 0; + XtSetArg(args[nargs], XtNstring, ""); nargs++; + XtSetValues(nameValWid, args, nargs); + } +} + + + + +/********************************Check for overwriting***********************/ +#include +#include +int checkOKwriteSeq(char *fn) +/* See if file exists */ +{ + struct stat buf; + return ( stat(fn,&buf) ); +} + +static void warningYesCallback(Widget w, + XtPointer client_data, XtPointer call_data) +/* +** A yes response to the +** warning about data not saved +*/ +{ + int *response = (int *) client_data; + + *response = 1; +} + +static void warningNoCallback(Widget w, + XtPointer client_data, XtPointer call_data) +/* +** A No response to the +** warning about data not saved +*/ +{ + int *response = (int *) client_data; + + *response = 0; +} + + +static int warning(Widget parentWid, String warn_message) +/* +** Warn that changes have been made but contig hasn't been saved. +*/ +{ + Widget dialog; + Arg args[10]; + int nargs; + int Response; + Widget warningWid, warnFormWid; + Position x, y; + + + + /* + Position the upper left hand corner of the popup at the + center of the parent widget. + */ + XtTranslateCoords(parentWid, + (Position) 0, (Position) 0, + &x, &y); + nargs = 0; + XtSetArg(args[nargs], XtNx, x); nargs++; + XtSetArg(args[nargs], XtNy, y); nargs++; + warningWid = XtCreatePopupShell("warning", transientShellWidgetClass, + parentWid, + args, nargs); + + /* Create the form */ + nargs = 0; + warnFormWid = XtCreateManagedWidget("warnForm", formWidgetClass, + warningWid, args, nargs); + + + + /* Create the form */ + nargs = 0; + XtSetArg(args[nargs],XtNlabel,warn_message); nargs++; + dialog = XtCreateManagedWidget("warnwid", dialogWidgetClass, + warnFormWid, args, nargs); + XawDialogAddButton(dialog,"Yes",warningYesCallback,(XtPointer)&Response); + XawDialogAddButton(dialog,"No",warningNoCallback,(XtPointer)&Response); + + /* This is a modal dialogue */ + Response = -1; + XtPopup(warningWid, XtGrabExclusive); + + while (Response==-1) + { + XEvent event; + + XtAppNextEvent(XtWidgetToApplicationContext(parentWid), &event); + XtDispatchEvent(&event); + } + + XtDestroyWidget(warningWid); + + return Response; +} + + + +int check_file_is_ok(Widget w, char *fn) +{ + if (!checkOKwriteSeq(fn)) { + return warning(w,"File already exists\nOK to overwrite?"); + } else + return 1; +} + +/********************************************************************/ + + + + + static void inputCancelCallback(Widget w, + XtPointer client_data, XtPointer call_data) + { Widget popupWid = (Widget) client_data; + + XtDestroyWidget(popupWid); + } + + + + + static void outputOKCallback(Widget w, + XtPointer client_data, XtPointer call_data) + { Widget popupWid, nameValWid, radioGroupWid, seqNameWid; + Widget includeHeaderWid; + char *fn, *seqName; + Boolean rc; + Boolean includeHeader; + Arg args[10]; + int nargs; + + popupWid = (Widget) client_data; + nameValWid = XtNameToWidget(popupWid, "ioForm.nameVal"); + radioGroupWid = XtNameToWidget(popupWid, "ioForm.formatBox.plainFmt"); + includeHeaderWid = XtNameToWidget(popupWid, "ioForm.includeHeader"); + seqNameWid = XtNameToWidget(XtParent(XtParent(popupWid)), "seqName"); + + nargs = 0; + XtSetArg(args[nargs], XtNlabel, &seqName); nargs++; + XtGetValues(seqNameWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNstring, &fn); nargs++; + XtGetValues(nameValWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNstate, &includeHeader); nargs++; + XtGetValues(includeHeaderWid, args, nargs); + + if (check_file_is_ok(w,fn)) { + rc=writeSeq(currSeq, fn, (strlen(r_fn)==0)?seqName:r_fn,includeHeader); + + if (rc) + { XtDestroyWidget(popupWid); + setDirty(currSeq, False); + } + else + { XBell(XtDisplay(popupWid), 100); + nargs = 0; + XtSetArg(args[nargs], XtNstring, ""); nargs++; + XtSetValues(nameValWid, args, nargs); + } + } else + XBell(XtDisplay(popupWid), 100); + } + + + + + static void outputCancelCallback(Widget w, + XtPointer client_data, XtPointer call_data) + { Widget popupWid = (Widget) client_data; + + XtDestroyWidget(popupWid); + } + + + + + static void checkOKCallback(Widget w, + XtPointer client_data, XtPointer call_data) + { Widget popupWid, parentWid; + + popupWid = (Widget) client_data; + parentWid = XtParent(popupWid); + + XtDestroyWidget(popupWid); + + /* + The user does want to overwrite the existing sequence. + Mark it as clean so `inputSeq' will dispose of it. + */ + setDirty(currSeq, False); + inputSeq(parentWid); + } + + + + + static void checkCancelCallback(Widget w, + XtPointer client_data, XtPointer call_data) + { Widget popupWid = (Widget) client_data; + + XtDestroyWidget(popupWid); + } + + + + + static void quitOKCallback(Widget w, + XtPointer client_data, XtPointer call_data) + { Widget popupWid = (Widget) client_data; + + XtDestroyWidget(popupWid); + + /* + The user does not want to save the existing sequence. + Quit the application. + */ + freeSeq(currSeq); + XtDestroyApplicationContext(XtWidgetToApplicationContext(w)); + exit(0); + } + + + + + static void quitCancelCallback(Widget w, + XtPointer client_data, XtPointer call_data) + { Widget popupWid = (Widget) client_data; + + XtDestroyWidget(popupWid); + + /* + The user does not want to quit the application. + */ + } + + static void searchCallback(Widget w, + XtPointer client_data, XtPointer call_data) + { Widget popupWid, nameValWid, NorigBasesWid, nextWid; + char *astring; + Arg args[10]; + int nargs; + int num,i,num_bases,baseNum; + int mag; + char *theSeq; + + popupWid = (Widget) client_data; + nameValWid = XtNameToWidget(popupWid, "ioForm.nameVal"); + NorigBasesWid = XtNameToWidget(XtParent(XtParent(popupWid)), "NorigBases"); + nextWid = XtNameToWidget(popupWid, "ioForm.next"); + + next_indices = 0; + num_matches = 0; + + /* + Get the name and type of the new one and read it in. + */ + nargs = 0; + XtSetArg(args[nargs], XtNstring, &astring); nargs++; + XtGetValues(nameValWid, args, nargs); + + + if (currSeq != NULLSeq) + { + /* astring != NULL, find the string that corresponds + to the app_resources.astring and center the display on that string, + change by lfw, 10/24/90 from this line to free line */ + + if (astring != NULL) { + + num = 1; /* num = 1 if astring is a basenum and 0 if alpha */ + for (i = 0; i < strlen(astring); i++) + if (!isdigit(astring[i])) { + num = 0; + break; + } + if (num==0) { + for (i = 0; i < strlen(astring); i++) + if (islower(astring[i])) astring[i]=toupper(astring[i]); + } + /* if any of the entries were alphabetic, the program + searches for a string */ + + if (num == 0) { + + num_bases = getNBases(currSeq,EdBases); + theSeq = (char *)calloc(num_bases,sizeof(char)); + for (i = 0; i < num_bases; i++) + theSeq[i] = getBase(currSeq, EdBases, i); + + num_matches = string_match(astring,strlen(astring),theSeq,num_bases, + 0, indices); + + if (num_matches == 0) baseNum = -5; + else { + baseNum = indices[0]; + next_indices++; + } + + free(theSeq); + + } + else { + sscanf(astring,"%d",&baseNum) ; +/* if (currSeq->bottom) baseNum = currSeq->NorigBases - 1 - baseNum;*/ + } + + mag = -5; /* by setting magnification to -5, the displaySeq + routine will go and back calculate the current + magnification */ + + if (baseNum != -5) { + displaySeq(currSeq, + ((baseNum!=NULLBaseNum) && + (baseNum>=0) && + (baseNum 1) { + nargs = 0; + XtSetArg(args[nargs], XtNlabel, "Next?"); nargs++; + XtSetValues(nextWid, args, nargs); + } + else + XtDestroyWidget(popupWid); + + } + else { + nargs = 0; + XtSetArg(args[nargs], XtNstring, "String not found"); nargs++; + XtSetValues(nameValWid, args, nargs); + } + } + } + else + XBell(XtDisplay(NorigBasesWid), 100); + } + + + + + static void searchNextCallback(Widget w, + XtPointer client_data, XtPointer call_data) + { Widget popupWid, nextWid; + Arg args[10]; + int nargs; + int baseNum; + int mag; + + popupWid = (Widget) client_data; + nextWid = XtNameToWidget(popupWid, "ioForm.next"); + + if (currSeq != NULLSeq) + { + mag = -5; /* by setting magnification to -5, the displaySeq + routine will go and back calculate the current + magnification */ + if (next_indices < num_matches) { + baseNum = indices[next_indices]; + next_indices++; + + nargs = 0; + XtSetArg(args[nargs], XtNlabel, "Next?"); nargs++; + XtSetValues(nextWid, args, nargs); + + displaySeq(currSeq, + ((baseNum!=NULLBaseNum) && + (baseNum>=0) && + (baseNum nmiss) return (0); + n_match = 0; + for (d = -nmiss; d <= n2 + nmiss - n1; d++){ + if (d < 0) istart = i_miss = -d; + else istart = i_miss = 0; + if (d > n2 - n1) { + iend = n2 - d; + i_miss += n1 + d - n2; + } + else iend = n1; + for (i = istart, j = d + i; i < iend; i++, j++) + if (seq1[i] != seq2[j] && ++i_miss > nmiss) goto nextd; +/* VOMIT! I want to PUKE!!!! who put this horrid goto here? */ + mtable[n_match][0] = d + istart; /* indices (assuming they start at + 0) of starting nucleotide in the + searched sequence */ + mtable[n_match][1] = istart; /* nucleotide position in the query + sequence where match starts (assuming + query index starts with 0) */ + mtable[n_match][2] = iend - istart; /* number of nucleotides in the + match */ + if (mtable[n_match][2]==n1) { + indices[n_match] = mtable[n_match][0]; + } + + mtable[n_match][3] = i_miss; /* number of mismatches */ + n_match++; /* number of matches */ + if (n_match >= 100) return(n_match); + + nextd:; + } + + return (n_match); + /* return(0); *//*return 0 if it got to this point; that means + it must have not found an exact match, so + you want the baseNum to be 0 in that case*/ +} + + + + + +/* ---- Exports ---- */ + + + + +void inputSeq(Widget parentWid) +/* + Set up a dialogue which will read in and display a sequence. +*/ +{ Widget inputWid, ioFormWid, ioPromptWid; + Widget namePromptWid, nameValWid; + Widget formatPromptWid, formatBoxWid, plainFmtWid, abiFmtWid, scfFmtWid; + Widget alfFmtWid; + Widget okWid, cancelWid; + Widget bottomFmtWid, topFmtWid; + Arg args[10]; + int nargs; + Position x, y; + Dimension width, height; + + + /* Add string to function bindings for our application actions */ + XtActionsRec actionTable[] = { {"CRAction", CRAction} }; + XtAppAddActions(XtWidgetToApplicationContext(parentWid), + actionTable, XtNumber(actionTable)); + + + /* + Check whether there is an existing sequence which + is dirty which needs saving. + */ + if (currSeq!=NULLSeq && isDirty(currSeq)) + { checkInputSeq(parentWid); + return; + } + + + /* + Position the upper left hand corner of the popup at the + center of the parent widget. + */ + nargs = 0; + XtSetArg(args[nargs], XtNwidth, &width); nargs++; + XtSetArg(args[nargs], XtNheight, &height); nargs++; + XtGetValues(parentWid, args, nargs); + XtTranslateCoords(parentWid, + (Position) (width / 2), (Position) (height / 2), + &x, &y); + + nargs = 0; + XtSetArg(args[nargs], XtNx, x); nargs++; + XtSetArg(args[nargs], XtNy, y); nargs++; + inputWid = XtCreatePopupShell("input", transientShellWidgetClass, + parentWid, + args, nargs); + + nargs = 0; + ioFormWid = XtCreateManagedWidget("ioForm", formWidgetClass, + inputWid, args, nargs); + + nargs = 0; + ioPromptWid = XtCreateManagedWidget("ioPrompt", labelWidgetClass, + ioFormWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, ioPromptWid); nargs++; + namePromptWid = XtCreateManagedWidget("namePrompt", labelWidgetClass, + ioFormWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, ioPromptWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, namePromptWid); nargs++; + XtSetArg(args[nargs], XtNeditType, XawtextEdit); nargs++; + nameValWid = XtCreateManagedWidget("nameVal", asciiTextWidgetClass, + ioFormWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, namePromptWid); nargs++; + formatPromptWid = XtCreateManagedWidget("formatPrompt", labelWidgetClass, + ioFormWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, namePromptWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, formatPromptWid); nargs++; + formatBoxWid = XtCreateManagedWidget("formatBox", boxWidgetClass, + ioFormWid, args, nargs); + + nargs = 0; + plainFmtWid = XtCreateManagedWidget("plainFmt", toggleWidgetClass, + formatBoxWid, + args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNradioGroup, plainFmtWid); nargs++; + abiFmtWid = XtCreateManagedWidget("abiFmt", toggleWidgetClass, + formatBoxWid, + args, nargs); + + + nargs = 0; + XtSetArg(args[nargs], XtNradioGroup, abiFmtWid); nargs++; + alfFmtWid = XtCreateManagedWidget("alfFmt", toggleWidgetClass, + formatBoxWid, + args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNradioGroup, abiFmtWid); nargs++; + scfFmtWid = XtCreateManagedWidget("scfFmt", toggleWidgetClass, + formatBoxWid, + args, nargs); + + + nargs = 0; + bottomFmtWid = XtCreateManagedWidget("bottomFmt", toggleWidgetClass, + formatBoxWid, + args, nargs); + nargs = 0; + XtSetArg(args[nargs], XtNradioGroup, bottomFmtWid); nargs++; + topFmtWid = XtCreateManagedWidget("topFmt", toggleWidgetClass, + formatBoxWid, + args, nargs); + + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, formatPromptWid); nargs++; + okWid = XtCreateManagedWidget("OK", commandWidgetClass, + ioFormWid, args, nargs); + XtAddCallback(okWid, XtNcallback, inputOKCallback, (XtPointer) inputWid); + + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, formatPromptWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, okWid); nargs++; + cancelWid = XtCreateManagedWidget("Cancel", commandWidgetClass, + ioFormWid, args, nargs); + XtAddCallback(cancelWid, XtNcallback, + inputCancelCallback, (XtPointer) inputWid); + + + /* nameVal (text) widget gets the keyboard focus */ + XtSetKeyboardFocus(ioFormWid, nameValWid); + + + /* This is a modal dialogue */ + XtPopup(inputWid, XtGrabExclusive); +} + + + + +void outputSeq(Widget parentWid, String defaultFileName) +/* + Save the current sequence using the deafult file name, if given. +*/ +{ Widget outputWid, ioFormWid, ioPromptWid; + Widget namePromptWid, nameValWid; + Widget includeHeaderWid,plainFormatWid; + Widget okWid, cancelWid; + Arg args[10]; + int nargs; + Position x, y; + Dimension width, height; + + + /* Add string to function bindings for our application actions */ + XtActionsRec actionTable[] = { {"CRAction", CRAction} }; + XtAppAddActions(XtWidgetToApplicationContext(parentWid), + actionTable, XtNumber(actionTable)); + + + /* If there is no current sequence, then return */ + if (currSeq==NULLSeq) return; + + + /* + Position the upper left hand corner of the popup at the + center of the parent widget. + */ + nargs = 0; + XtSetArg(args[nargs], XtNwidth, &width); nargs++; + XtSetArg(args[nargs], XtNheight, &height); nargs++; + XtGetValues(parentWid, args, nargs); + XtTranslateCoords(parentWid, + (Position) (width / 2), (Position) (height / 2), + &x, &y); + nargs = 0; + XtSetArg(args[nargs], XtNx, x); nargs++; + XtSetArg(args[nargs], XtNy, y); nargs++; + outputWid = XtCreatePopupShell("output", transientShellWidgetClass, + parentWid, + args, nargs); + + /* Create the form */ + nargs = 0; + ioFormWid = XtCreateManagedWidget("ioForm", formWidgetClass, + outputWid, args, nargs); + + nargs = 0; + ioPromptWid = XtCreateManagedWidget("ioPrompt", labelWidgetClass, + ioFormWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, ioPromptWid); nargs++; + namePromptWid = XtCreateManagedWidget("namePrompt", labelWidgetClass, + ioFormWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, ioPromptWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, namePromptWid); nargs++; + XtSetArg(args[nargs], XtNeditType, XawtextEdit); nargs++; + XtSetArg(args[nargs], XtNstring, defaultFileName); nargs++; + nameValWid = XtCreateManagedWidget("nameVal", asciiTextWidgetClass, + ioFormWid, args, nargs); + /* Set the insertion point after the text - dodgy method used */ + XawTextSetInsertionPoint(nameValWid, MAXLONG); + + + nargs = 0; + XtSetArg(args[nargs], XtNstate, True); nargs++; + XtSetArg(args[nargs], XtNfromVert, namePromptWid); nargs++; + XtSetArg(args[nargs], XtNlabel, "Include Header"); nargs++; + includeHeaderWid = XtCreateManagedWidget("includeHeader", toggleWidgetClass, + ioFormWid, + args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNstate, False); nargs++; + XtSetArg(args[nargs], XtNfromVert, namePromptWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, includeHeaderWid); nargs++; + XtSetArg(args[nargs], XtNlabel, "Plain Format"); nargs++; + XtSetArg(args[nargs], XtNradioGroup, includeHeaderWid); nargs++; + plainFormatWid = XtCreateManagedWidget("plainFormat", toggleWidgetClass, + ioFormWid, + args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, includeHeaderWid); nargs++; + okWid = XtCreateManagedWidget("OK", commandWidgetClass, + ioFormWid, args, nargs); + XtAddCallback(okWid, XtNcallback, outputOKCallback, (XtPointer) outputWid); + + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, includeHeaderWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, okWid); nargs++; + cancelWid = XtCreateManagedWidget("Cancel", commandWidgetClass, + ioFormWid, args, nargs); + XtAddCallback(cancelWid, XtNcallback, + outputCancelCallback, (XtPointer) outputWid); + + /* nameVal (text) widget gets the keyboard focus */ + XtSetKeyboardFocus(ioFormWid, nameValWid); + + + /* This is a modal dialogue */ + XtPopup(outputWid, XtGrabExclusive); +} + + + + +void quitApplication(Widget parentWid) +/* + This function must be called to exit the application. + It ensures any current sequence is saved. + */ +{ Widget ioFormWid, ioPromptWid, quitWid; + Widget okWid, cancelWid; + Arg args[10]; + int nargs; + Position x, y; + Dimension width, height; + + + /* Add string to function bindings for our application actions */ + XtActionsRec actionTable[] = { {"CRAction", CRAction} }; + XtAppAddActions(XtWidgetToApplicationContext(parentWid), + actionTable, XtNumber(actionTable)); + + + /* + Check whether there is an existing sequence which + is dirty which needs saving. + */ + if (currSeq==NULLSeq || !isDirty(currSeq)) + { free(currSeq); + XtDestroyApplicationContext(XtWidgetToApplicationContext(parentWid)); + exit(0); + } + + + /* + Position the upper left hand corner of the popup at the + center of the parent widget. + */ + nargs = 0; + XtSetArg(args[nargs], XtNwidth, &width); nargs++; + XtSetArg(args[nargs], XtNheight, &height); nargs++; + XtGetValues(parentWid, args, nargs); + XtTranslateCoords(parentWid, + (Position) (width / 2), (Position) (height / 2), + &x, &y); + + nargs = 0; + XtSetArg(args[nargs], XtNx, x); nargs++; + XtSetArg(args[nargs], XtNy, y); nargs++; + quitWid = XtCreatePopupShell("quit", transientShellWidgetClass, + parentWid, + args, nargs); + + nargs = 0; + ioFormWid = XtCreateManagedWidget("ioForm", formWidgetClass, + quitWid, args, nargs); + + nargs = 0; + ioPromptWid = XtCreateManagedWidget("ioPrompt", labelWidgetClass, + ioFormWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, ioPromptWid); nargs++; + okWid = XtCreateManagedWidget("OK", commandWidgetClass, + ioFormWid, args, nargs); + XtAddCallback(okWid, XtNcallback, + quitOKCallback, (XtPointer) quitWid); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, ioPromptWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, okWid); nargs++; + cancelWid = XtCreateManagedWidget("Cancel", commandWidgetClass, + ioFormWid, args, nargs); + XtAddCallback(cancelWid, XtNcallback, + quitCancelCallback, (XtPointer) quitWid); + + + /* This is a modal dialogue */ + XtPopup(quitWid, XtGrabExclusive); +} + + + + +void initialDisplayedSeq(Widget toplevelWid, + char *format, char *fn, + int baseNum, int mag, char *astring, + char *enzyme, int bottom) +/* + This function may be called once, after the application + has been realised, to specify a sequence to be displayed + initially. If it is not called, no sequence is initially + displayed. + */ +{ Arg args[10]; + int nargs; + int i; + char NBasesString[10]; + char *theSeq; + AppData app_data; + char enzString[100]; + + + int num_bases; + Widget seqNameWid = XtNameToWidget(toplevelWid, "mainForm.seqName"); + Widget NorigBasesWid = XtNameToWidget(toplevelWid, "mainForm.NorigBases"); + num_matches = 0; + next_indices = 0; + + XtGetApplicationResources(seqNameWid, (XtPointer) &app_data, + resources, XtNumber(resources), + NULL, 0); + +#ifndef AUTO_CLIP + app_data.enz='\0'; +#endif + + /* enzyme will already equal either the app_resources.enz or + app_data.enz, lfw 05.02.91*/ + if (enzyme!=NULL) + strcpy(enzString,enzyme); + /* if (app_data.enz != NULL) + strcpy(enzString,app_data.enz);*/ + else *enzString = '\0'; + + + + /* read a sequence */ + currSeq = readSeq(fn,bottom,enzString,format); + + if (currSeq != NULLSeq) + { + /* + Set the `file name' and `number of bases' labels. + */ + nargs = 0; + XtSetArg(args[nargs], XtNlabel, fn); nargs++; + XtSetValues(seqNameWid, args, nargs); + nargs = 0; + sprintf(NBasesString, "%d", getNBases(currSeq, OrigBases)); + XtSetArg(args[nargs], XtNlabel, NBasesString); nargs++; + XtSetValues(NorigBasesWid, args, nargs); + + /* astring != NULL, find the string that corresponds + to the app_resources.astring and center the display on that string, + change by lfw, 10/24/90 from this line to free line */ + + if (astring != NULL) { + num_bases = getNBases(currSeq,EdBases); + theSeq = (char *)calloc(num_bases,sizeof(char)); + for (i = 0; i < num_bases; i++) + theSeq[i] = getBase(currSeq, EdBases, i); + + num_matches = string_match(astring,strlen(astring),theSeq,num_bases, + 0, indices); + baseNum = indices[0]; + if (num_matches == 0) baseNum = 0; + + free(theSeq); + } + + displaySeq(currSeq, + ((baseNum!=NULLBaseNum) && + (baseNum>=0) && + (baseNum /* IMPORT: Widget */ +#include + +/* ---- Global to ted.c and dialogues.c --- */ +extern char o_fn[200]; /* added by lfw, so a default output filename + could be specified */ +extern char r_fn[200]; /* added by sd, so a default raw data filename + could be specified */ + +/* ---- Exports ---- */ + +extern void inputSeq(Widget parentWid); +/* + Set up a dialogue which will read in and display a new sequence. +*/ + + +extern void outputSeq(Widget parentWid, String defaultFileName); +/* + Save the current sequence using the default file name, if given. +*/ + +extern void inputSearchString(Widget parentWid); +/* + Set up a dialogue which will read in and search for a string. +*/ + +extern void quitApplication(Widget parentWid); +/* + This function must be called to exist the application. + It ensures any current sequence is saved. +*/ + + +extern void initialDisplayedSeq(Widget toplevelWid, + char *format, char *fn, + int baseNum, int mag, char *astring, + char *enzyme, int bottom); +/* + This function may be called once, after the application + has been realised, to specify a sequence to be displayed + initially. If it is not called, no sequence is initially + displayed. +*/ + +extern void information(Widget w); +/* + Display useful sequence and trace information +*/ + +extern int string_match(char *seq1, int n1, char *seq2, int n2, + int nmiss, int *indices); + +#endif /*_dialogues_h*/ diff --git a/src/ted/display.c b/src/ted/display.c new file mode 100644 index 0000000..a0c9537 --- /dev/null +++ b/src/ted/display.c @@ -0,0 +1,801 @@ +/* + Title: display + + File: display.c + Purpose: Sequence display and editing + Last update: Friday 15 May 1992 +*/ + + +/* + The display is created by a call to CreateDisplay. It principally + consists of two components: the first, created by a call to + createBasesDisplay displays the original and edited bases, the + second, created by a call to createTracesDisplay, displays the + traces. + + Editing is done by stream: as the client presses keys and buttons + these must be interpreted as editing keystrokes. + + The viewport widget does not itself hand out resize events, so we + create a dummy (graph) widget in the same patch, connected in the + same way to the walls of the form, to collect resize events and + allow us to adjust scalings for the graphs within the viewport. + This is slightly dodgy and should be redone some other way. + + Changes to this program by lfw: + module displaySeq + Added the parameter mag to the parameters + passed to the subroutine + Changed to default magnification to 30% + by changing the call to setGraphWidth + to be 30 rather than -1. And by + allowing the call to setGraphWidth + to be specified by the user which + comes from the main calling program + via the mag parameter + + Changes made by sd: + Added scaleUp and scaleDown buttons + callbacks + + SD 15-May-1992 + editSeqEvents(): DEC Xlib function XlookupString didn't always work with + a buffer of length of 1, even when only one character returned. Made + buffer larger - now eight characters (doesn't work with <=four) +*/ + + + + +/* ---- Includes ---- */ + +#include "display.h" +#include "traceDisplay.h" /* IMPORT: createTraceDisplay, displayTrace, + unDisplayTrace, setTraceWidth */ +#include "basesDisplay.h" /* IMPORT: createBasesDisplay, displayBases, + unDisplayBases, setBasesWidth */ +#include "Graph.h" +#include "seq.h" /* IMPORT: Seq, NULLSeq, getNPoints */ + +#include /* IMPORT: isgraph */ + +#include +#include +#include +#include +#include +#include +#include +#include +#include + + + + +/* ---- Constants ---- */ + + +#define MaxDimension 32767 /* By definition, 2^16-1 */ +#define MaxMagCharSpace 5 /* At maximum magnification, the distance in + characters between each base */ +#define ViewportFudge 10 /* A guess of the space required within the + viewport for the graph to fit */ + + +/* the following allows us to get the default magnification from the + app_defaults file, Xted */ +typedef struct +{ int magnif; + String enz; +} AppData, *AppDataPtr; + + +#define XtNmagnif "magnif" +#define XtCMagnif "Magnif" +#define XtNenz "enz" +#define XtCEnz "Enz" + +static XtResource resources[] = { + { XtNmagnif, + XtCMagnif, + XtRInt, + sizeof(int), + XtOffset(AppDataPtr, magnif), + XtRImmediate, + NULL + }, + { XtNenz, + XtCEnz, + XtRString, + sizeof(String), + XtOffset(AppDataPtr, enz), + XtRImmediate, + NULL + } + }; + + + + + + + + +/* ---- Statics ---- */ + +/* Current sequence information */ +static Seq currSeq = NULLSeq; +static int NPoints; +static int NedBases; + +/* Viewport widget */ +static Widget viewportWid; +static Widget vpFormWid; +static Widget dummyWid; + +static Dimension charWidth; /* As used by basesDisplay */ +static Dimension minGraphWidth; /* All characters displayed + (depends on width of parent viewport) */ +static Dimension maxGraphWidth; /* Bases spaced out with `MaxMagCharSpace' + blanks (depends on char size and + NorigBases) */ +static Dimension graphWidth; /* Current width of the graph */ +static Dimension plotEdgeOffset; + + +/* Editing state toggles */ +static Widget togWid; /* The group of toggles */ + + +/* Magnification widget */ +static Widget magWid; + + + + +/* ---- Internal routines ---- */ + + + + +static void setGraphWidth(int percent) +/* + Set the widths of the graphs to be `percent' between + the minimum and maximum widths. + The current ``centre of interest'' is maintained. +*/ +{ int nargs; + Arg args[10]; + float centre, topOfThumb, shown; + Widget hScrollWid = XtNameToWidget(viewportWid, "horizontal"); + + /* + The ``centre of interest'' is the middle of the displayed + graph. Percentagewise, this is given by `topOfThumb+show/2'. + */ + nargs=0; + XtSetArg(args[nargs], XtNtopOfThumb, &topOfThumb); nargs++; + XtSetArg(args[nargs], XtNshown, &shown); nargs++; + XtGetValues(hScrollWid, args, nargs); + centre = topOfThumb+shown/2; + + /* + Set the displays to the required width + */ + graphWidth = ((maxGraphWidth-minGraphWidth) * percent)/100 + minGraphWidth; + XawFormDoLayout(vpFormWid, False); + nargs = 0; + XtSetArg(args[nargs], XtNwidth, graphWidth); nargs++; + setBasesWidth(graphWidth); + setTraceWidth(graphWidth); + XawFormDoLayout(vpFormWid, True); + + /* + We have changed the size of the form widget within the viewport + widget. The viewport does not define where exactly the form + will now sit, so we manually reset the ``centre of interest'', + keeping `shown' at its (new) value. + */ + nargs=0; + XtSetArg(args[nargs], XtNtopOfThumb, &topOfThumb); nargs++; + XtSetArg(args[nargs], XtNshown, &shown); nargs++; + XtGetValues(hScrollWid, args, nargs); + topOfThumb = centre-shown/2; + XawScrollbarSetThumb(hScrollWid, topOfThumb, shown); + XtCallCallbacks(hScrollWid, XtNjumpProc, &topOfThumb); +} + + + + +static void editSeqEvent(XEvent *eventP) +/* + `eventP' (a button or key press) is interpreted as a sequence + editing command. +*/ +{ + char buffer[8]; + KeySym keySym; + int charCount; + XComposeStatus compose; + + switch (eventP->type) + { case KeyPress: + charCount = XLookupString((XKeyEvent *)eventP, buffer, 8, + &keySym, &compose); + switch (keySym) + { case XK_Right: + moveCaretRight(); + break; + + case XK_Left: + moveCaretLeft(); + break; + + case XK_Delete: + /* Delete the base to the left of the caret */ + if (deleteBase(currSeq, getCaret())) + { baseDeleted(); + NedBases--; + } + else + XBell(XtDisplay(viewportWid), 100); + break; + + default: + if (charCount==1 && isgraph(buffer[0])) + { /* Insert the base to the right of the caret */ + if (insertBase(currSeq, buffer[0], getCaret())) + { baseInserted(); + NedBases++; + } + else + XBell(XtDisplay(viewportWid), 100); + } + break; + } + break; + + case ButtonPress: + { /* Move the caret to where the pointer is */ + int point = pixelToPoint(eventP->xbutton.x); + if (point<0) moveCaretTo(-1); + else if (point>NPoints-1) moveCaretTo(NedBases-1); + else + { int b = pointToBaseNum(currSeq, EdBases, point); + moveCaretTo((b == NULLBaseNum) ? NedBases-1 : b-1); + } + break; + } + } +} + + + + +static void adjustLEvent(XEvent *eventP) +/* + `eventP' (a button or key press) is interpreted as a command + to adjust the left cutoff. +*/ +{ char buffer[1]; + KeySym keySym; + int charCount; + XComposeStatus compose; + int leftCutoff, rightCutoff; + + switch (eventP->type) + { case KeyPress: + charCount = XLookupString((XKeyEvent *)eventP, buffer, 1, + &keySym, &compose); + switch (keySym) + { case XK_Right: + /* Move the cutoff right one */ + getCutoffs(currSeq, &leftCutoff, &rightCutoff); + if (leftCutoff0) leftCutoff--; + (void) setCutoffs(currSeq, leftCutoff, rightCutoff); + basesCutoffChange(); + traceCutoffChange(); + break; + } + break; + + case ButtonPress: + { int point, baseNum; + + /* Move the cutoff to where the pointer is */ + point = pixelToPoint(eventP->xbutton.x); + if (point<0) baseNum = 0; + else if (point>NPoints-1) baseNum = NedBases; + else + { baseNum = pointToBaseNum(currSeq, EdBases, point); + + baseNum = (baseNum == NULLBaseNum) + ? NedBases + : baseNum; + } + getCutoffs(currSeq, &leftCutoff, &rightCutoff); + setCutoffs(currSeq, baseNum, rightCutoff); + basesCutoffChange(); + traceCutoffChange(); + break; + } + } +} + + + + +static void adjustREvent(XEvent *eventP) +/* + `eventP' (a button or key press) is interpreted as a command + to adjust the right cutoff. +*/ +{ char buffer[1]; + KeySym keySym; + int charCount; + XComposeStatus compose; + int leftCutoff, rightCutoff; + + switch (eventP->type) + { case KeyPress: + charCount = XLookupString((XKeyEvent *)eventP, buffer, 1, + &keySym, &compose); + switch (keySym) + { case XK_Right: + /* Move the cutoff right one */ + getCutoffs(currSeq, &leftCutoff, &rightCutoff); + if (rightCutoff>0) rightCutoff--; + (void) setCutoffs(currSeq, leftCutoff, rightCutoff); + basesCutoffChange(); + traceCutoffChange(); + break; + + case XK_Left: + /* Move the cutoff left one */ + getCutoffs(currSeq, &leftCutoff, &rightCutoff); + if (rightCutoffxbutton.x); + if (point<0) baseNum = 0; + else if (point>NPoints-1) baseNum = NedBases; + else + { baseNum = pointToBaseNum(currSeq, EdBases, point); + + baseNum = (baseNum == NULLBaseNum) + ? NedBases + : baseNum; + } + getCutoffs(currSeq, &leftCutoff, &rightCutoff); + setCutoffs(currSeq, leftCutoff, NedBases-baseNum); + basesCutoffChange(); + traceCutoffChange(); + break; + } + } +} + + + + +/* ---- Callbacks ---- */ + + +static void resizeCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ Dimension width; + int mag; + Arg args[10]; + int nargs; + + nargs = 0; + XtSetArg(args[nargs], XtNwidth, &width); nargs++; + XtGetValues(viewportWid, args, nargs); + + /* Back calculate what the current magnification is */ + mag = ((graphWidth-minGraphWidth)*100) / (maxGraphWidth-minGraphWidth); + + /* Adjust the minimum magnification */ + minGraphWidth = width-ViewportFudge; + graphWidth = minGraphWidth; + + /* Set us to the old magnification, but with the new dimensions */ + setGraphWidth(mag); +} + + + + +static void magJumpCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ float percent = *((float *) call_data); + + setGraphWidth((int) (percent*100)); +} + + + + +static void magScrollCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ int position = (int) call_data; + float topOfThumb; + int nargs; + Arg args[10]; + + nargs=0; + XtSetArg(args[nargs], XtNtopOfThumb, &topOfThumb); nargs++; + XtGetValues(wid, args, nargs); + + /* Move the thumb by 1% in the appropriate direction */ + if (position>0) + { topOfThumb += .01; + if (topOfThumb > 1.0) topOfThumb = 1.0; + } + else + { topOfThumb -= .01; + if (topOfThumb < 0.0) topOfThumb = 0.0; + } + + XawScrollbarSetThumb(wid, topOfThumb, -1.0); + + setGraphWidth((int) (topOfThumb*100)); +} + + +static void scaleUpCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ + incScaleFactor(); +} + +static void scaleDownCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ + decScaleFactor(); +} + + +static void complementCallback(Widget wid, + XtPointer client_data, XtPointer call_data) +{ + complement_seq(currSeq); + /* force redisplay of everything in viewport widget */ + redisplayTraces(); + redisplayBases(); + /* shift caret */ + moveCaretTo(currSeq->NedBases - getCaret() - 2); + + { /* set viewport position */ + Cardinal nargs; + float topOfThumb, shown; + Arg args[2]; + Widget hScrollWid = XtNameToWidget(viewportWid, "horizontal"); + nargs=0; + XtSetArg(args[nargs], XtNtopOfThumb, &topOfThumb); nargs++; + XtSetArg(args[nargs], XtNshown, &shown); nargs++; + XtGetValues(hScrollWid, args, nargs); + topOfThumb = 1.0 - shown - topOfThumb; + XawScrollbarSetThumb(hScrollWid, topOfThumb, shown); + XtCallCallbacks(hScrollWid, XtNjumpProc, &topOfThumb); + } + + /* set strand widget */ + { + Widget strandWid; + strandWid = XtNameToWidget(XtParent(wid), "strand"); + set_strand_label(strandWid,currSeq); + + } + +} + + + +/* ---- Exports ---- */ + + +void createDisplay(Widget parentWid, Widget fromVertWid) +/* + Create the display within the Form widget `parentWid' + with the XtNfromVert constraint `fromVertWid'. + No sequence is initally displayed. +*/ +{ Widget labelWid, basesWid; + Widget scaleUpWid, scaleDownWid; + Widget compWid; + Arg args[10]; + int nargs; + + /* The magnification slider */ + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + labelWid = XtCreateManagedWidget("maglab", labelWidgetClass, + parentWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, labelWid); nargs++; + magWid = XtCreateManagedWidget("magscr", scrollbarWidgetClass, + parentWid, args, nargs); + XtAddCallback(magWid, XtNjumpProc, magJumpCallback, NULL); + XtAddCallback(magWid, XtNscrollProc, magScrollCallback, NULL); + + + + /* The editing mode toggles */ + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, magWid); nargs++; + labelWid = XtCreateManagedWidget("edmodelab", labelWidgetClass, + parentWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, labelWid); nargs++; + togWid = XtCreateManagedWidget("adjustL", toggleWidgetClass, + parentWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, togWid); nargs++; + XtSetArg(args[nargs], XtNradioGroup, togWid); nargs++; + togWid = XtCreateManagedWidget("edSeq", toggleWidgetClass, + parentWid, args, nargs); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, togWid); nargs++; + XtSetArg(args[nargs], XtNradioGroup, togWid); nargs++; + togWid = XtCreateManagedWidget("adjustR", toggleWidgetClass, + parentWid, args, nargs); + + + /* for vertical scaling */ + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, togWid); nargs++; + scaleDownWid = XtCreateManagedWidget("scaleDown", commandWidgetClass, + parentWid, args, nargs); + XtAddCallback(scaleDownWid, XtNcallback, scaleDownCallback, NULL); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, scaleDownWid); nargs++; + scaleUpWid = XtCreateManagedWidget("scaleUp", commandWidgetClass, + parentWid, args, nargs); + XtAddCallback(scaleUpWid, XtNcallback, scaleUpCallback, NULL); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, fromVertWid); nargs++; + XtSetArg(args[nargs], XtNfromHoriz, scaleUpWid); nargs++; + compWid = XtCreateManagedWidget("switch", commandWidgetClass, + parentWid, args, nargs); + XtAddCallback(compWid, XtNcallback, complementCallback, NULL); + + + /* The main display */ + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, labelWid); nargs++; + XtSetArg(args[nargs], XtNforceBars, True); nargs++; + viewportWid = XtCreateManagedWidget("viewport", viewportWidgetClass, + parentWid, args, nargs); + + /* + The minimum graph width is the viewport width minus a + random amount to allow for spacing between the viewport + and the graph, borders etc. + The initial graph width is this minimum width. + */ + { Dimension vpWidth; + + nargs = 0; + XtSetArg(args[nargs], XtNwidth, &vpWidth); nargs++; + XtGetValues(viewportWid, args, nargs); + minGraphWidth = vpWidth-ViewportFudge; + graphWidth = minGraphWidth; + } + + nargs = 0; + vpFormWid = XtCreateManagedWidget("vpForm", formWidgetClass, + viewportWid, args, nargs); + + basesWid = createBasesDisplay(vpFormWid, NULL, graphWidth); + getBasesFontInfo(&plotEdgeOffset, &charWidth); + createTraceDisplay(vpFormWid,basesWid,graphWidth, plotEdgeOffset); + + nargs = 0; + XtSetArg(args[nargs], XtNfromVert, magWid); nargs++; + dummyWid = XtCreateManagedWidget("dummy", graphWidgetClass, + parentWid, args, nargs); + XtAddCallback(dummyWid, XtNresizeCallback, resizeCallback, NULL); +} + + + + +void displaySeq(Seq seq, int baseNum, int mag) +/* + Display the sequence `seq' for editing. + If baseNum is not equal to NULLBaseNum, display at 30% (or mag) + magnification centered on baseNum. +*/ +{ AppData app_data; + + + + XtGetApplicationResources(magWid, (XtPointer) &app_data, + resources, XtNumber(resources), + NULL, 0); + + + if (seq == NULLSeq) return; + currSeq = seq; + + displayBases(seq,baseNum); + displayTrace(seq); + + + /* + The maximum graph width is such to allow for a `MaxMagCharSpace' + spacing between bases. + + There is a limit on Dimension sizes, noting that the graph we + generate has to fit into a Viewport. + */ + { Dimension maxWidth; + int NorigBases; + + NorigBases = getNBases(currSeq, OrigBases); + maxWidth = ((NorigBases-1) * charWidth * MaxMagCharSpace) + + (2 * plotEdgeOffset); + maxGraphWidth = + (maxWidth>MaxDimension-ViewportFudge) ? MaxDimension-ViewportFudge + : (maxWidth 100) { + setGraphWidth(0); + XawScrollbarSetThumb(magWid, 0.3, -1.0); + } + else { + setGraphWidth(mag); + XawScrollbarSetThumb(magWid, (float)mag/100.0, -1.0); + } + } + else + { /* Centred on baseNum, half magnification */ + Widget hScrollWid = XtNameToWidget(viewportWid, "horizontal"); + float topOfThumb; + + /* Set the `centre of interest' on baseNum. Zero `shown'. */ +/* + topOfThumb = (float)baseNum / (float)(NedBases-1); +*/ + topOfThumb = (float) baseNumToPoint(seq, OrigBases,baseNum) / (float) NPoints; + XawScrollbarSetThumb(hScrollWid, topOfThumb, 0.0); + /* Set the actual magnification */ + +/* the original program automatically set the magnification to 30% if + the user specified a baseNum other than NULL, this section was added + by LaDeana Hillier, 9/27/90, to allow the user to set their own + magnification when calling up ted. */ + + if (mag==-5) /* back calculate it */ + mag = ((graphWidth-minGraphWidth)*100) / (maxGraphWidth-minGraphWidth); + if (app_data.magnif==0) app_data.magnif = 30; + + if (mag <= 0 || mag > 100) { + setGraphWidth(app_data.magnif); + /* Set the magnification scrollbar */ + XawScrollbarSetThumb(magWid, (float)(app_data.magnif)/100.0, -1.0); + } + else { + setGraphWidth(mag); + XawScrollbarSetThumb(magWid, (float)mag/100.0, -1.0); + } + } +} + + + + +void unDisplaySeq() +/* + Cease displaying the current sequence (if any). +*/ +{ currSeq = NULLSeq; + + + /* + Reset the magnification to minimum. + The thumb is left its default size. + */ + XawScrollbarSetThumb(magWid, 0.0, -1.0); + + unDisplayBases(); + unDisplayTrace(); + /* + The initial width is the minimum, so everything is visible. + This also clears the graphs. + */ + setGraphWidth(0); +} + + + + +Seq displayedSeq() +/* + Return the currently displayed sequence, or NULL + if none is being displayed. +*/ +{ return(currSeq); +} + + + + +void userEvent(XEvent *eventP) +{ String currTog; + + if (currSeq == NULLSeq) return; + + /* + Find which of the toggles is currently set + */ + currTog = (String) XawToggleGetCurrent(togWid); + + if (strcmp(currTog, "adjustL") == 0) + { /* Adjust the left cutoff */ + adjustLEvent(eventP); + } + else if (strcmp(currTog, "adjustR") == 0) + { /* Adjust the right cutoff */ + adjustREvent(eventP); + } + else + { /* Edit the sequence itself */ + editSeqEvent(eventP); + } +} + + diff --git a/src/ted/display.h b/src/ted/display.h new file mode 100644 index 0000000..a93070f --- /dev/null +++ b/src/ted/display.h @@ -0,0 +1,68 @@ +/* + Title: display + + File: display.h + Purpose: Sequence display and editing + Last update: Tue Jun 5 1990 +*/ + + +/* +*/ + + + + +/* ---- Includes ---- */ + +#include "seq.h" /* IMPORT: Seq */ + +#include /* IMPORT: Widget */ + + + + +/* ---- Exports ---- */ + + +extern void createDisplay(Widget parentWid, Widget superiorWid); +/* + Create the display within the Form widget `parent', + underneath `superiorWid'. + No sequence is initially displayed. +*/ + + +extern void displaySeq(Seq seq, int baseNum, int mag); +/* + Display the sequence `seq' for editing. + If baseNum is not equal to -1, display at half magnification + centered on baseNum. (unless the user has also set mag) +*/ + + +extern void unDisplaySeq(); +/* + Cease displaying the current sequence (if any). +*/ + + +extern Seq displayedSeq(); +/* + Return the currently displayed sequence, or NULLSeq + if none is being displayed. +*/ + + +extern void userEvent(XEvent *eventP); +/* + Handle the (key or button) event generated in a subdisplay. +*/ + + + + + + + + diff --git a/src/ted/fpoint.c b/src/ted/fpoint.c new file mode 100644 index 0000000..cd930c1 --- /dev/null +++ b/src/ted/fpoint.c @@ -0,0 +1,54 @@ +/*#include */ +extern double log ( double x ) ; +extern double exp ( double x ) ; +#define IEEE + +float int_to_float(int in) +/* +** interpret the integer in as a +** floating point number in IEEE format +*/ +{ + /* + Assume `in' is stored as a float according to the + ANSI IEEE 754-1985 standard. See the tables below: + + s = sign ( 1 bit) + e = biased exponent (8 bits) + f = fraction (23 bits) + + floating point number = (-1)^s 2^(e-127) 1.f + + Bits Name Content + 31 Sign 1 iff number is negative + 23-30 Exponent Eight-Bit exponent, biased by 127 + 0-22 Fraction 23-bit fraction component of normalised significant. + The "one" bit is "hidden" + + If IEEE floating point format is supported on your machine... + ensure there is a #define IEEE somewhere. + */ + +#ifdef IEEE + union { + int i; + float f; + } cvt; + cvt.i = in; + return cvt.f; +#else + int fraction; + int exponent; + int sign; + + fraction = in & ( (1<<23)-1 ); + exponent = (in >> 23) & ( (1<<8)-1 ); + sign = (in >> 31); + + return + (float) ( + (sign?-1.0:1.0) * + exp ( log ( (double) 2.0) * (double) (exponent - 127 - 23) ) * + (double) ((1<<23)+fraction)) ; +#endif +} diff --git a/src/ted/fpoint.h b/src/ted/fpoint.h new file mode 100644 index 0000000..065c5df --- /dev/null +++ b/src/ted/fpoint.h @@ -0,0 +1,6 @@ +#ifndef _fpoint_h +#define _fpoint_h + +extern float int_to_float(int in); + +#endif /* _fpoint_h */ diff --git a/src/ted/help.c b/src/ted/help.c new file mode 100644 index 0000000..c446e79 --- /dev/null +++ b/src/ted/help.c @@ -0,0 +1,128 @@ +/* + Title: help + + File: help.c + Purpose: Help + Last update: Fri Jun 15 1990 +*/ + + +/* ---- Includes ---- */ +#include + +#include "help.h" + +#include +#include +#include +#include +#include +#include + + + + +/* ---- Statics ---- */ + +static Widget helpShellWid; +static Boolean helpShellMapped=False; + + + + +/* ---- Callbacks ---- */ + + +static void removeCallback(Widget w, + XtPointer client_data, XtPointer call_data) +{ XtPopdown(helpShellWid); + helpShellMapped=False; +} + + + + +/* ---- Exports ---- */ + + +Widget createHelpShell(Widget toplevelWid) +/* + Create the help shell as a child of `toplevelWid'. + The shell is not initially mapped. +*/ +{ Widget formWid, removeWid, textWid; + String fn; + Arg args[10]; + int nargs; + + char apology[] = "Sorry, I couldn't find the \"ted.help\" file.\n\ +This should either be in \"/usr/X11/app-defaults\" or somewhere\n\ +referred to by the \"XFILESEARCHPATH\" environment variable."; + + + /* + The toplevel shell widget holds a form widget + */ + helpShellWid = XtCreatePopupShell("Help", topLevelShellWidgetClass, + toplevelWid, + NULL, (Cardinal) 0); + + formWid = XtCreateManagedWidget("form", formWidgetClass, helpShellWid, + NULL, (Cardinal) 0); + + + nargs = 0; + removeWid = XtCreateManagedWidget("rmButton", commandWidgetClass, + formWid, args, nargs); + XtAddCallback(removeWid, XtNcallback, removeCallback, NULL); + + + /* + Find the help file. + */ + fn = XtResolvePathname(XtDisplay(toplevelWid), + "app-defaults", /* type */ + "ted", /* file name */ + ".help", /* suffix */ + NULL, /* path */ + NULL, (Cardinal)0, /* substitutions */ + NULL /* file predicate */ + ); + + + /* + If we found the file display it, else an apology. + */ + nargs = 0; + if (fn != NULL) + { XtSetArg(args[nargs], XtNtype, XawAsciiFile); nargs++; + XtSetArg(args[nargs], XtNstring, fn); nargs++; + } + else + { XtSetArg(args[nargs], XtNtype, XawAsciiString); nargs++; + XtSetArg(args[nargs], XtNstring, apology); nargs++; + } + XtSetArg(args[nargs], XtNfromVert, removeWid); nargs++; + XtSetArg(args[nargs], XtNscrollVertical, XawtextScrollAlways); nargs++; + textWid = XtCreateManagedWidget("text", asciiTextWidgetClass, + formWid, args, nargs); + + + if (fn != NULL) XtFree(fn); + + + return(helpShellWid); +} + + + + +void help() +/* + Display help modeless dialogue. +*/ +{ if (!helpShellMapped) + { XtPopup(helpShellWid, XtGrabNone); + helpShellMapped = True; + } +} diff --git a/src/ted/help.h b/src/ted/help.h new file mode 100644 index 0000000..5778471 --- /dev/null +++ b/src/ted/help.h @@ -0,0 +1,38 @@ +#ifndef _help_h +#define _help_h + + +/* + Title: help + + File: help.h + Purpose: Help + Last update: Fri Jun 15 1990 +*/ + + + +/* ---- Includes ---- */ + +#include /* IMPORT: Widget */ + + + + +/* ---- Exports ---- */ + + +extern Widget createHelpShell(Widget toplevelWid); +/* + Create the help shell as a child of `toplevelWid'. + The shell is not initially mapped. +*/ + + +extern void help(); +/* + Display help modeless dialogue. +*/ + + +#endif /*_help_h*/ diff --git a/src/ted/info.c b/src/ted/info.c new file mode 100644 index 0000000..f264a2e --- /dev/null +++ b/src/ted/info.c @@ -0,0 +1,123 @@ +/* + Title: info + + File: info.c + Purpose: Info + Last update: Tue Nov 10 1992 +*/ + + +/* ---- Includes ---- */ +#include + +#include "info.h" +#include "seq.h" + +#include +#include +#include +#include +#include +#include + + + + +/* ---- Statics ---- */ + + + + + +/* ---- Callbacks ---- */ + + + + +static void get_info_string(Seq seq, char *seq_info) +{ + switch (seq->format) { + case ABIFormat: + case SCFFormat: + if (seq->info != NULL) { + sprintf(seq_info,"%s\n",seq->info); + break; + } + case ALFFormat: + default: + strcpy(seq_info,"No information for this sequence\n"); + break; + } +} + +/*ARGSUSED*/ +static void +DestroyPopupPrompt(Widget widget, XtPointer client_data, XtPointer call_data) +{ + + Widget popup = XtParent( (Widget) client_data); + XtDestroyWidget(popup); +} + + +/* ---- Exports ---- */ + + +/* ARGSUSED */ +void info(Widget button, Seq seq) +{ + Arg args[5]; + Widget popup; + Position x, y; + Dimension width, height; + Cardinal n; + Widget apane,apane1,apane2; + char seq_info[500]; + + if (seq == NULLSeq) return; + /* + * This will position the upper left hand corner of the popup at the + * center of the widget which invoked this callback, which will also + * become the parent of the popup. + */ + + get_info_string(seq,seq_info); + + n = 0; + XtSetArg(args[0], XtNwidth, &width); n++; + XtSetArg(args[1], XtNheight, &height); n++; + XtGetValues(button, args, n); + XtTranslateCoords(button, (Position) (width / 2), (Position) (height / 2), + &x, &y); + + x -= 350; + n = 0; + XtSetArg(args[n], XtNx, x); n++; + XtSetArg(args[n], XtNy, y); n++; + + popup = XtCreatePopupShell("prompt", transientShellWidgetClass, button, + args, n); + + apane = XtCreateManagedWidget("apane", formWidgetClass, popup ,NULL, 0); + apane2 = XtVaCreateManagedWidget("apane2", asciiTextWidgetClass,apane, + XtNeditType, XawtextRead, + XtNwidth, 400, + XtNheight, 80, + XtNstring, seq_info, + XtNscrollVertical, XawtextScrollAlways, + NULL); + + apane1 = XtVaCreateManagedWidget("apane3", commandWidgetClass,apane, + XtNeditType, XawtextEdit, + XtNwidth, 400, + XtNheight, 20, + XtNfromVert, apane2, + XtNlabel, "Cancel", + NULL); + + XtAddCallback(apane1, XtNcallback, DestroyPopupPrompt, (XtPointer)apane); + XtPopup(popup, XtGrabNone); + +} + + diff --git a/src/ted/info.h b/src/ted/info.h new file mode 100644 index 0000000..9d4161d --- /dev/null +++ b/src/ted/info.h @@ -0,0 +1,30 @@ +#ifndef _info_h +#define _info_h + + +/* + Title: info + + File: info.h + Purpose: Info + Last update: Fri Jun 15 1990 +*/ + + + +/* ---- Includes ---- */ + +#include /* IMPORT: Widget */ + + + + +/* ---- Exports ---- */ + +extern void info(); +/* + Display info modeless dialogue. +*/ + + +#endif /*_info_h*/ diff --git a/src/ted/mach-io.c b/src/ted/mach-io.c new file mode 100644 index 0000000..9b78205 --- /dev/null +++ b/src/ted/mach-io.c @@ -0,0 +1,238 @@ +/* +** Machine independant io: +** For reading and writing to big-endian and little-endian files +** +** Routines available: +** be_write_int_1() +** be_write_int_2() +** be_write_int_4() +** be_read_int_1() +** be_read_int_2() +** be_read_int_4() +** le_write_int_1() +** le_write_int_2() +** le_write_int_4() +** le_read_int_1() +** le_read_int_2() +** le_read_int_4() +** +** All routine return: +** 0 - an error has occurred during io operation +** 1 - value suggessfully read or written +*/ + +#include +#include "mach-io.h" + + + + +/**********************************************************************/ +/* IO for big-endian files */ +/**********************************************************************/ + +int be_write_int_1(FILE *fp, uint_1 *i1) +/* +** Write a big-endian int_1 +*/ +{ + if (fwrite(i1, sizeof(uint_1), 1, fp) != 1) return (0); + return (1); +} + + + + + +int be_write_int_2(FILE *fp, uint_2 *i2) +/* +** Write a big-endian int_2 +*/ +{ + uint_1 buf[sizeof(int_2)]; + + buf[0] = (uint_1) (*i2>>8)&255; + buf[1] = (uint_1) *i2&255; + if (fwrite(buf, sizeof(buf), 1, fp) != 1) return (0); + return (1); +} + + + + + +int be_write_int_4(FILE *fp, uint_4 *i4) +/* +** Write a big-endian int_4 +*/ +{ + uint_1 buf[sizeof(*i4)]; + + + buf[0] = (uint_1) (*i4>>24)&255; + buf[1] = (uint_1) (*i4>>16)&255; + buf[2] = (uint_1) (*i4>>8)&255; + buf[3] = (uint_1) *i4&255; + if (fwrite(buf, sizeof(buf), 1, fp) != 1) return (0); + return (1); +} + + + + + +int be_read_int_1(FILE *fp, uint_1 *i1) +/* +** Read a big-endian int_1 +*/ +{ + if (fread(i1, sizeof(uint_1), 1, fp) != 1) return (0); + return (1); +} + + + + + +int be_read_int_2(FILE *fp, uint_2 *i2) +/* +** Read a big-endian int_2 +*/ +{ + uint_1 buf[sizeof(int_2)]; + + if (fread(buf, sizeof(buf), 1, fp) != 1) return (0); + *i2 = + (((uint_2)buf[1]) + + ((uint_2)buf[0]<<8)); + return (1); +} + + + + + +int be_read_int_4(FILE *fp, uint_4 *i4) +/* +** Read a big-endian int_4 +*/ +{ + uint_1 buf[sizeof(int_4)]; + + if (fread(buf, sizeof(buf), 1, fp) != 1) return (0); + *i4 = + (((uint_4)buf[3]) + + ((uint_4)buf[2]<<8) + + ((uint_4)buf[1]<<16) + + ((uint_4)buf[0]<<24)); + return (1); +} + + + + + + + + + + +/**********************************************************************/ +/* IO for little-endian files */ +/**********************************************************************/ + +int le_write_int_1(FILE *fp, uint_1 *i1) +/* +** Write a little-endian int_1 +*/ +{ + if (fwrite(i1, sizeof(uint_1), 1, fp) != 1) return (0); + return (1); +} + + + + + +int le_write_int_2(FILE *fp, uint_2 *i2) +/* +** Write a little-endian int_2 +*/ +{ + uint_1 buf[sizeof(int_2)]; + + buf[1] = (uint_1) (*i2>>8)&255; + buf[0] = (uint_1) *i2&255; + if (fwrite(buf, sizeof(buf), 1, fp) != 1) return (0); + return (1); +} + + + + + +int le_write_int_4(FILE *fp, uint_4 *i4) +/* +** Write a little-endian int_4 +*/ +{ + uint_1 buf[sizeof(int_4)]; + + buf[3] = (uint_1) (*i4>>24)&255; + buf[2] = (uint_1) (*i4>>16)&255; + buf[1] = (uint_1) (*i4>>8)&255; + buf[0] = (uint_1) *i4&255; + if (fwrite(buf, sizeof(buf), 1, fp) != 1) return (0); + return (1); +} + + + + + +int le_read_int_1(FILE *fp, uint_1 *i1) +/* +** Read a little-endian int_1 +*/ +{ + if (fread(i1, sizeof(uint_1), 1, fp) != 1) return (0); + return (1); +} + + + + + +int le_read_int_2(FILE *fp, uint_2 *i2) +/* +** Read a little-endian int_2 +*/ +{ + uint_1 buf[sizeof(int_2)]; + + if (fread(buf, sizeof(buf), 1, fp) != 1) return (0); + *i2 = + (((uint_2)buf[0]) + + ((uint_2)buf[1]<<8)); + return (1); +} + + + + + +int le_read_int_4(FILE *fp, uint_4 *i4) +/* +** Read a little-endian int_4 +*/ +{ + uint_1 buf[sizeof(int_4)]; + + if (fread(buf, sizeof(buf), 1, fp) != 1) return (0); + *i4 = + (((uint_4)buf[0]) + + ((uint_4)buf[1]<<8) + + ((uint_4)buf[2]<<16) + + ((uint_4)buf[3]<<24)); + return (1); +} diff --git a/src/ted/mach-io.h b/src/ted/mach-io.h new file mode 100644 index 0000000..5783df3 --- /dev/null +++ b/src/ted/mach-io.h @@ -0,0 +1,115 @@ +#ifndef _mach_io_h +#define _mach_io_h +/* +** Machine independant io +** For reading and writing to big-endian and little-endian files +** +** Routines available: +** be_write_int_1() +** be_write_int_2() +** be_write_int_4() +** be_read_int_1() +** be_read_int_2() +** be_read_int_4() +** le_write_int_1() +** le_write_int_2() +** le_write_int_4() +** le_read_int_1() +** le_read_int_2() +** le_read_int_4() +** +** All routine return: +** 0 - an error has occurred during io operation +** 1 - value successfully read or written +*/ + +#include + +typedef char int_1; +typedef short int_2; +typedef int int_4; +typedef unsigned char uint_1; +typedef unsigned short uint_2; +typedef unsigned int uint_4; + +/**********************************************************************/ +/* IO for big-endian files */ +/**********************************************************************/ + +extern int be_write_int_1(FILE *fp, uint_1 *i1); +/* +** Write a big-endian int_1 +*/ + +extern +int be_write_int_2(FILE *fp, uint_2 *i2); +/* +** Write a big-endian int_2 +*/ + +extern +int be_write_int_4(FILE *fp, uint_4 *i4); +/* +** Write a big-endian int_4 +*/ + +extern +int be_read_int_1(FILE *fp, uint_1 *i1); +/* +** Read a big-endian int_1 +*/ + +extern +int be_read_int_2(FILE *fp, uint_2 *i2); +/* +** Read a big-endian int_2 +*/ + +extern +int be_read_int_4(FILE *fp, uint_4 *i4); +/* +** Read a big-endian int_4 +*/ + +/**********************************************************************/ +/* IO for little-endian files */ +/**********************************************************************/ + +extern +int le_write_int_1(FILE *fp, uint_1 *i1); +/* +** Write a little-endian int_1 +*/ + +extern +int le_write_int_2(FILE *fp, uint_2 *i2); +/* +** Write a little-endian int_2 +*/ + +extern +int le_write_int_4(FILE *fp, uint_4 *i4); +/* +** Write a little-endian int_4 +*/ + +extern +int le_read_int_1(FILE *fp, uint_1 *i1); +/* +** Read a little-endian int_1 +*/ + +extern +int le_read_int_2(FILE *fp, uint_2 *i2); +/* +** Read a little-endian int_2 +*/ + +extern +int le_read_int_4(FILE *fp, uint_4 *i4); +/* +** Read a little-endian int_4 +*/ + + +#endif /* _mach_io_h */ diff --git a/src/ted/makefile-alpha b/src/ted/makefile-alpha new file mode 100644 index 0000000..e571a12 --- /dev/null +++ b/src/ted/makefile-alpha @@ -0,0 +1,89 @@ +# +# Makefile for ted (trace editor) - Alpha fork +# + +# +#Turning on the AUTO_CLIP switch allows ted to automatically +#select a left (using the -enzyme option or the .enzyme in the +#Xted file) and right cutoff on your sequence (using 2 out of +#5 N's) +# +# Turning on the SAVE_EDITS switch allows the user to maintain +# copies of their edits, i.e. when you open up ted on a file +# that you have already edited, the old edits show up in the +# ted edit window. The user may also call up any of their +# past editing sessions. +# +#Turning on the DEF_OUT switch makes +# trace_name.seq the default output file name +# +#SWITCHES = -DAUTO_CLIP -DDEF_OUT -DSAVE_EDITS +SWITCHES = -DAUTO_CLIP + +# +# Programs made by this makefile +# +PROGS = ted + +# +# Where the wild things are +# +SRC = . + +# +# Compiler - debug on +# +CC= cc +INCLUDES = -I$(SRC) +CFLAGS= -g -std1 -ieee_with_inexact $(SWITCHES) $(INCLUDES) +LIBS= -lXaw -lXt -lXmu -lXext -lX11 -ldnet_stub +# +LD= cc +LDFLAGS= -g -non_shared + +# +# Object files +# +TEDOBJS=\ + ted.o\ + dialogues.o\ + seq.o\ + seqIOPlain.o\ + seqIOABI.o\ + help.o\ + display.o\ + traceDisplay.o\ + basesDisplay.o\ + Graph.o\ + seqIOEdit.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqOutput.o\ + opp.o\ + info.o\ + fpoint.o\ + mach-io.o\ + seqRead.o + + +# +# Linking +# +ted: $(TEDOBJS) + $(LD) $(LDFLAGS) -o $@ $(TEDOBJS) $(LIBS) + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o : $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + + +# +# Special dependencies +# +clean: + rm -f ted $(TEDOBJS) *~ *.aux *.log *.bbl *.blg *.dvi *.ps + +all: $(PROGS) + diff --git a/src/ted/makefile-dec b/src/ted/makefile-dec new file mode 100644 index 0000000..a3bc627 --- /dev/null +++ b/src/ted/makefile-dec @@ -0,0 +1,92 @@ +# +# Makefile for ted (trace editor) - DEC Ultrix +# + +# +#Turning on the AUTO_CLIP switch allows ted to automatically +#select a left (using the -enzyme option or the .enzyme in the +#Xted file) and right cutoff on your sequence (using 2 out of +#5 N's) +# +# Turning on the SAVE_EDITS switch allows the user to maintain +# copies of their edits, i.e. when you open up ted on a file +# that you have already edited, the old edits show up in the +# ted edit window. The user may also call up any of their +# past editing sessions. +# +#Turning on the DEF_OUT switch makes +# trace_name.seq the default output file name +# +#SWITCHES = -DAUTO_CLIP -DDEF_OUT -DSAVE_EDITS +SWITCHES = -DAUTO_CLIP + +# +# Programs made by this makefile +# +PROGS = ted + +# +# Where the wild things are +# +SRC = . + +# +# Compiler - debug on +# +CC= c89 +INCLUDES = -I$(SRC) -I/usr/include/mit +CFLAGS= -g -common $(SWITCHES) $(INCLUDES) +LIBS= -lXaw -lXt -lXmu -lXext -lX11 +# +# If you have gcc-2.x, should link with it +LD= c89 +LDFLAGS= -g +# cc -Bstatic, gcc2.x -static, otherwise nothing +LDLIBS= $(LIBS) + +# +# Object files +# +TEDOBJS=\ + ted.o\ + dialogues.o\ + seq.o\ + seqIOPlain.o\ + seqIOABI.o\ + help.o\ + display.o\ + traceDisplay.o\ + basesDisplay.o\ + Graph.o\ + seqIOEdit.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqOutput.o\ + opp.o\ + info.o\ + fpoint.o\ + mach-io.o\ + seqRead.o + + +# +# Linking +# +ted: $(TEDOBJS) + $(LD) $(LDFLAGS) -o $@ $(TEDOBJS) $(LDLIBS) + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o : $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + + +# +# Special dependencies +# +clean: + rm -f ted $(TEDOBJS) *~ *.aux *.log *.bbl *.blg *.dvi *.ps + +all: $(PROGS) + diff --git a/src/ted/makefile-sgi b/src/ted/makefile-sgi new file mode 100644 index 0000000..86516ef --- /dev/null +++ b/src/ted/makefile-sgi @@ -0,0 +1,91 @@ +# +# Makefile for ted (trace editor) - SGI fork +# + +SHELL=/bin/sh + +# +#Turning on the AUTO_CLIP switch allows ted to automatically +#select a left (using the -enzyme option or the .enzyme in the +#Xted file) and right cutoff on your sequence (using 2 out of +#5 N's) +# +# Turning on the SAVE_EDITS switch allows the user to maintain +# copies of their edits, i.e. when you open up ted on a file +# that you have already edited, the old edits show up in the +# ted edit window. The user may also call up any of their +# past editing sessions. +# +#Turning on the DEF_OUT switch makes +# trace_name.seq the default output file name +# +#SWITCHES = -DAUTO_CLIP -DDEF_OUT -DSAVE_EDITS +SWITCHES = -DAUTO_CLIP + +# +# Programs made by this makefile +# +PROGS = ted + +# +# Where the wild things are +# +SRC = . + +# +# Compiler - debug on +# +CC= cc +INCLUDES = -I$(SRC) +CFLAGS= -g -ansi -prototypes $(SWITCHES) $(INCLUDES) +LIBS= -lXaw -lXt -lXmu -lXext -lX11 +# +LD= cc +LDFLAGS= -g + +# +# Object files +# +TEDOBJS=\ + ted.o\ + dialogues.o\ + seq.o\ + seqIOPlain.o\ + seqIOABI.o\ + help.o\ + display.o\ + traceDisplay.o\ + basesDisplay.o\ + Graph.o\ + seqIOEdit.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqOutput.o\ + opp.o\ + info.o\ + fpoint.o\ + mach-io.o\ + seqRead.o + + +# +# Linking +# +ted: $(TEDOBJS) + $(LD) $(LDFLAGS) -o $@ $(TEDOBJS) $(LIBS) + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o : $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + + +# +# Special dependencies +# +clean: + rm -f ted $(TEDOBJS) *~ *.aux *.log *.bbl *.blg *.dvi *.ps + +all: $(PROGS) + diff --git a/src/ted/makefile-solaris b/src/ted/makefile-solaris new file mode 100644 index 0000000..76aea9c --- /dev/null +++ b/src/ted/makefile-solaris @@ -0,0 +1,93 @@ +# +# Makefile for ted (trace editor) - SunOS fork +# + +# +#Turning on the AUTO_CLIP switch allows ted to automatically +#select a left (using the -enzyme option or the .enzyme in the +#Xted file) and right cutoff on your sequence (using 2 out of +#5 N's) +# +# Turning on the SAVE_EDITS switch allows the user to maintain +# copies of their edits, i.e. when you open up ted on a file +# that you have already edited, the old edits show up in the +# ted edit window. The user may also call up any of their +# past editing sessions. +# +#Turning on the DEF_OUT switch makes +# trace_name.seq the default output file name +# +#SWITCHES = -DAUTO_CLIP -DDEF_OUT -DSAVE_EDITS +SWITCHES = -DAUTO_CLIP + +# +# Programs made by this makefile +# +PROGS = ted + +# +# Where the wild things are +# +SRC = . + +# +# Compiler - debug on +# +CC= gcc +GCCWARNINGS = -Wreturn-type -Wunused -Wswitch -Wcomment -Wformat +INCLUDES = -I$(SRC) -I/usr/openwin/include +CFLAGS= -g -ansi -pedantic $(GCCWARNINGS) $(SWITCHES) $(INCLUDES) +LIBS= -lXaw -lXt -lXmu -lXext -lX11 -lm +# +# If you have gcc-2.x, should link with it +LD= gcc +LDFLAGS= -g # -static # causes major dl problems +# cc -Bstatic, gcc2.x -static, otherwise nothing +LDLIBS= $(LIBS) + +# +# Object files +# +TEDOBJS=\ + ted.o\ + dialogues.o\ + seq.o\ + seqIOPlain.o\ + seqIOABI.o\ + help.o\ + display.o\ + traceDisplay.o\ + basesDisplay.o\ + Graph.o\ + seqIOEdit.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqOutput.o\ + opp.o\ + info.o\ + fpoint.o\ + mach-io.o\ + seqRead.o + + +# +# Linking +# +ted: $(TEDOBJS) + $(LD) $(LDFLAGS) -o $@ $(TEDOBJS) $(LDLIBS) + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o : $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + + +# +# Special dependencies +# +clean: + rm -f ted $(TEDOBJS) *~ *.aux *.log *.bbl *.blg *.dvi *.ps + +all: $(PROGS) + diff --git a/src/ted/makefile-sun b/src/ted/makefile-sun new file mode 100644 index 0000000..c751fd5 --- /dev/null +++ b/src/ted/makefile-sun @@ -0,0 +1,94 @@ +# +# Makefile for ted (trace editor) - SunOS fork +# + +# +#Turning on the AUTO_CLIP switch allows ted to automatically +#select a left (using the -enzyme option or the .enzyme in the +#Xted file) and right cutoff on your sequence (using 2 out of +#5 N's) +# +# Turning on the SAVE_EDITS switch allows the user to maintain +# copies of their edits, i.e. when you open up ted on a file +# that you have already edited, the old edits show up in the +# ted edit window. The user may also call up any of their +# past editing sessions. +# +#Turning on the DEF_OUT switch makes +# trace_name.seq the default output file name +# +#SWITCHES = -DAUTO_CLIP -DDEF_OUT -DSAVE_EDITS +SWITCHES = -DAUTO_CLIP + +# +# Programs made by this makefile +# +PROGS = ted + +# +# Where the wild things are +# +SRC = . + +# +# Compiler - debug on +# +CC= gcc +GCCWARNINGS = -Wreturn-type -Wunused -Wswitch -Wcomment -W -Wformat \ + -Wuninitialized +INCLUDES = -I$(SRC) +CFLAGS= -g -ansi -pedantic $(GCCWARNINGS) $(SWITCHES) $(INCLUDES) +LIBS= -lXaw -lXt -lXmu -lXext -lX11 +# +# If you have gcc-2.x, should link with it +LD= gcc +LDFLAGS= -g +# cc -Bstatic, gcc2.x -static, otherwise nothing +LDLIBS= -static $(LIBS) + +# +# Object files +# +TEDOBJS=\ + ted.o\ + dialogues.o\ + seq.o\ + seqIOPlain.o\ + seqIOABI.o\ + help.o\ + display.o\ + traceDisplay.o\ + basesDisplay.o\ + Graph.o\ + seqIOEdit.o\ + seqIOALF.o\ + seqIOSCF.o\ + seqOutput.o\ + opp.o\ + info.o\ + fpoint.o\ + mach-io.o\ + seqRead.o + + +# +# Linking +# +ted: $(TEDOBJS) + $(LD) $(LDFLAGS) -o $@ $(TEDOBJS) $(LDLIBS) + +# +# Files requiring simple C compilation (thing.c -> thing.o) +# +%.o : $(SRC)/%.c + $(CC) $(CFLAGS) -c $< + + +# +# Special dependencies +# +clean: + rm -f ted $(TEDOBJS) *~ *.aux *.log *.bbl *.blg *.dvi *.ps + +all: $(PROGS) + diff --git a/src/ted/mystdlib.h b/src/ted/mystdlib.h new file mode 100644 index 0000000..e92c64b --- /dev/null +++ b/src/ted/mystdlib.h @@ -0,0 +1,33 @@ +/* + Title: stdlib + + File: mystdlib.h + Purpose: ANSII C stdlib.h replacement + Last update: Thu May 24 1990 +*/ + + +/* + The SUN does not provide the ANSII C stdlib.h header file, so + so this file provides some of the definitions which should be + found there. + + Note that we cannot override existing type definitions. +*/ + + + + +/* String processing functions */ +extern double strtod(char *str, char **ptr); +extern double atof(char *str); +extern long strtol(char *str, char **ptr, int base); + + +/* Control functions */ +extern void exit(int status); + + +/* Storage allocation */ +extern char *malloc(unsigned size); +extern char *calloc(unsigned elt_count, unsigned elt_size); diff --git a/src/ted/opp.c b/src/ted/opp.c new file mode 100644 index 0000000..7fe9809 --- /dev/null +++ b/src/ted/opp.c @@ -0,0 +1,128 @@ +/* + Title: opp.c + + File: opp.c + Purpose: code for complementing sequences + + Last update: Tue Jan 15 1991 + + 15.01.90 SD Taken from seqIOEdit.c +*/ + + +/* ---- Imports ---- */ +#include "opp.h" +#include "seq.h" + + +/* ---- Globals ---- */ +char opp[256]; /* complement of any given base */ + +/* ---- Exports ---- */ +void oppInitialize() +{ int i; + + for (i = 0; i<256; i++) opp[i]='-'; + + /* RMD 31/12/90 'N' -> '-' above. + removed 'N' and 'n' entries below and added reciprocal + 'K' and 'N' entries as for full Staden table */ + + opp['A'] = 'T'; + opp['G'] = 'C'; + opp['T'] = 'A'; + opp['C'] = 'G'; + opp['a'] = 't'; + opp['g'] = 'c'; + opp['t'] = 'a'; + opp['c'] = 'g'; + opp['D'] = 'H'; + opp['H'] = 'D'; + opp['V'] = 'B'; + opp['B'] = 'V'; + opp['K'] = 'N'; + opp['N'] = 'K'; + opp['L'] = 'M'; + opp['M'] = 'L'; + opp['5'] = '6'; + opp['6'] = '5'; + opp['R'] = 'Y'; + opp['Y'] = 'R'; + opp['7'] = '7'; + opp['8'] = '8'; +} + + +void complement_seq(Seq seq) +/* +** Complement and reverse bases and traces +*/ +{ + int2 temp_int2; + TRACE *temp_TRACEptr; + char temp_char; + int temp_int; + int i; + + /* swap */ +#define swap(A,B,I) ( (I)=(A), (A)=(B), (B)=(I) ) + + /* complement and reverse traces */ + if (seq->mode == BasesAndTraces) { + /* swap traces A<->T and C<->G */ + swap(seq->traceA,seq->traceT,temp_TRACEptr); + swap(seq->traceC,seq->traceG,temp_TRACEptr); + + /* reverse points in traces */ + for (i=0;iNPoints/2;i++) { + swap(seq->traceA[i],seq->traceA[seq->NPoints-i-1],temp_int2); + swap(seq->traceC[i],seq->traceC[seq->NPoints-i-1],temp_int2); + swap(seq->traceG[i],seq->traceG[seq->NPoints-i-1],temp_int2); + swap(seq->traceT[i],seq->traceT[seq->NPoints-i-1],temp_int2); + } + } + /* complement the sequence */ + /* handle edited base */ + for (i=0;iNedBases;i++) { + if (seq->edits[i]<0) { + int k = (-seq->edits[i]); + seq->edBase[k] = opp[seq->edBase[k]]; + seq->edBasePos[k] = seq->NPoints - seq->edBasePos[k] - 1; + } else { + int k = seq->edits[i]; + seq->edits[i] = seq->NorigBases-k-1; + /* + * skip complement and mod of base position until later + * because not every orig base is necessarily represented in the + * edits array + */ + } + } + + /* handle original bases */ + for (i=0;iNorigBases;i++) { + seq->base[i] = opp[seq->base[i]]; + seq->basePos[i] = seq->NPoints - seq->basePos[i] - 1; + } + + /* reverse sequence */ + /* edits */ + for (i=0;iNedBases/2;i++) { + swap(seq->edits[i],seq->edits[seq->NedBases-i-1],temp_int2); + } + /* orig bases */ + for (i=0;iNorigBases/2;i++) { + swap(seq->base[i],seq->base[seq->NorigBases-i-1],temp_char); + swap(seq->basePos[i],seq->basePos[seq->NorigBases-i-1],temp_int2); + } + + + /* swap cutoffs */ + swap(seq->leftCutoff,seq->rightCutoff,temp_int); + + /* move caret ??? */ + + /* toggle strand */ + seq->bottom = !seq->bottom; +} + diff --git a/src/ted/opp.h b/src/ted/opp.h new file mode 100644 index 0000000..0852a95 --- /dev/null +++ b/src/ted/opp.h @@ -0,0 +1,37 @@ +#ifndef _opp_h +#define _opp_h + +/* + Title: opp + + File: opp.h + Purpose: Required for complimenting a sequence + Last update: Tue Jan 15 1991 + + 15.01.90 SD Taken from seqIOEdit.h + +*/ + +#include "seq.h" + +/* ---- Exports ---- */ + +extern char opp[256]; /* complement of any given base */ + +extern void oppInitialize(); + +/* initializes the array which stores the complement + of any of the Staden nucleotides or ambiguity + codes */ + + +void complement_seq(Seq seq); + +/* complement a sequence */ + +#endif /*_opp_h*/ + + + + + diff --git a/src/ted/scf.h b/src/ted/scf.h new file mode 100644 index 0000000..adde21d --- /dev/null +++ b/src/ted/scf.h @@ -0,0 +1,89 @@ +/* + * File: scf.h + * Version: + * + * Author: Simon Dear + * MRC Laboratory of Molecular Biology + * Hills Road + * Cambridge CB2 2QH + * United Kingdom + * + * Description: file structure definitions for SCF file + * + * Created: 19 November 1992 + * Updated: + * + */ + +#ifndef _SCF_H_ +#define _SCF_H_ + +#include "mach-io.h" + +#define SCF_MAGIC (((((uint_4)'.'<<8)+(uint_4)'s'<<8)+(uint_4)'c'<<8)+(uint_4)'f') + +#define VERSION 2.00 + +/* + * Type definition for the Header structure + */ +typedef struct { + uint_4 magic_number; /* SCF_MAGIC */ + uint_4 samples; /* Number of elements in Samples matrix */ + uint_4 samples_offset; /* Byte offset from start of file */ + uint_4 bases; /* Number of bases in Bases matrix */ + uint_4 bases_left_clip; /* Number of bases in left clip (vector)*/ + uint_4 bases_right_clip; /* Number of bases in right clip (unreliable) */ + uint_4 bases_offset; /* Byte offset from start of file */ + uint_4 comments_size; /* Number of bytes in Comment section */ + uint_4 comments_offset; /* Byte offset from start of file */ + char version[4]; /* "version.revision" */ + uint_4 sample_size; /* precision of samples (in bytes) */ + uint_4 code_set; /* uncertainty codes used */ + uint_4 spare[20]; /* Unused */ +} Header; + +#define CSET_DEFAULT 0 /* {A,C,G,T,-} */ +#define CSET_STADEN 1 +#define CSET_NC_IUB 2 +#define CSET_ALF 3 /* extended NC_IUB */ +#define CSET_ABI 4 /* {A,C,G,T,N} */ + +/* + * Type definition for the Sample data + */ +typedef unsigned char byte; +typedef struct { + byte sample_A; /* Sample for A trace */ + byte sample_C; /* Sample for C trace */ + byte sample_G; /* Sample for G trace */ + byte sample_T; /* Sample for T trace */ +} Samples1; +typedef struct { + unsigned short sample_A; /* Sample for A trace */ + unsigned short sample_C; /* Sample for C trace */ + unsigned short sample_G; /* Sample for G trace */ + unsigned short sample_T; /* Sample for T trace */ +} Samples2; + +/* + * Type definition for the sequence data + */ +typedef struct { + uint_4 peak_index; /* Index into Samples matrix for base position */ + byte prob_A; /* Probability of it being an A */ + byte prob_C; /* Probability of it being an C */ + byte prob_G; /* Probability of it being an G */ + byte prob_T; /* Probability of it being an T */ + char base; /* Base called */ + byte spare[3]; /* Spare */ +} Bases; + + +/* + * Type definition for the comments + */ +typedef char Comments; /* Zero terminated list of \n separated entries */ + + +#endif /*_SCF_H_*/ diff --git a/src/ted/seq.c b/src/ted/seq.c new file mode 100644 index 0000000..87a7a96 --- /dev/null +++ b/src/ted/seq.c @@ -0,0 +1,544 @@ +/* + Title: seq + + File: seq.c + Purpose: Sequence data type + Last update: Tue Nov 10 1992 +*/ + + +/* + The Seq data type is designed so that it can hold a varying degree + of information about sequences, yet have a single set of calls + to access the data. + + The edited sequence has a limit of MaxEdits changes relative to + the original sequence. If entry M in the edited sequence is the + same as entry N in the original sequence then edits[M]=N. If + entry M is changed, then its value is negative and is a reference + into the newBase and newBasePos arrays (thus position 0 in these + arrays is unused). Initially, all the entries in newBasePos are + set to NULLPoint. The representation can be summarised thus + (where => is logical implication): + + getNBases(seq, EdBases) = seq->NedBases + + getBase(seq, EdBases, n) = + (seq->edits[n] >= 0) => seq->base[seq->edits[n]] + ~(seq->edits[n] >= 0) => seq->edBase[-(seq->edits[n])] + + baseNumToPoint(seq, EdBases, n) = + (seq->edits[n] >= 0) => seq->basePos[seq->edits[n]] + ~(seq->edits[n] >= 0) => seq->edBasePos[-(seq->edits[n])] + + The main problem with this representation is that an insertion + (or deletion) requires, on average, copying of half of the edits + array up (or down). Given that we have ~1000 bases this probably + OK. + + A problem which has not been considered well enough is how we deal + with several bases located at the same point. + + There are plenty of assumptions around that both the number of + bases and the number of points will fit into an int2, a short. + + The only changes made by lfw to this module is the addition of + two subroutines: isBottom, setBottom. +*/ + + + + +/* ---- Includes ---- */ + +#include "seq.h" + +#include +/* #include "mystdlib.h" *//* IMPORT: malloc, calloc */ + + + + +/* ---- Exports ---- */ + + + + +/* ---- General sequence manipulation ---- */ + + + + +Seq newSeq(Mode mode, int2 numPoints, int2 numBases) +/* + Allocate a new sequence, with the given sizes. + The sequence is initially clean with no bases cutoff. + If `mode' is BasesOnly, `numPoints' is ignored. + Returns NULLSeq on failure. +*/ +{ Seq seq = NULLSeq; + size_t int2Size = sizeof(int2); + + + /* Allocate the body of the sequence */ + if ((seq = (Seq) malloc(sizeof(SeqData))) == NULL) return(NULLSeq); + + /* + Initialise the body, all pointers are set to NULL so we can + happily call `freeSeq' + */ + seq->dirty = False; + seq->leftCutoff = 0; + seq->rightCutoff = 0; + seq->bottom = False; + + seq->traceC = NULL; + seq->traceA = NULL; + seq->traceG = NULL; + seq->traceT = NULL; + + seq->base = NULL; + seq->basePos = NULL; + + seq->edits = NULL; + seq->edBase = NULL; + seq->edBasePos = NULL; + + seq->info = NULL; + + /* Allocate space for the bases */ + if (((seq->base = malloc(numBases)) == NULL)|| + ((seq->basePos =(int2 *)calloc(numBases,int2Size)) == NULL)|| + + ((seq->edits =(int2 *)calloc(numBases+MaxEdits,int2Size)) == NULL)|| + ((seq->edBase = malloc(MaxEdits)) == NULL)|| + ((seq->edBasePos=(int2 *)calloc(MaxEdits,int2Size)) == NULL)) + { + freeSeq(seq); + return(NULLSeq); + } + + /* If needed, allocate space for the traces */ + if (mode == BasesAndTraces) + if (((seq->traceC =(TRACE *)calloc(numPoints,int2Size)) == NULL)|| + ((seq->traceA =(TRACE *)calloc(numPoints,int2Size)) == NULL)|| + ((seq->traceG =(TRACE *)calloc(numPoints,int2Size)) == NULL)|| + ((seq->traceT =(TRACE *)calloc(numPoints,int2Size)) == NULL)) + { + freeSeq(seq); + return(NULLSeq); + } + + return(seq); +} + + + + +void freeSeq(Seq seq) +/* + Free a sequence created by one of the above routines. + + ANSI C is happy to free a NULL pointer. +*/ +{ if (seq == NULLSeq) return; + + if (seq->traceC != NULL) free(seq->traceC); + if (seq->traceA != NULL) free(seq->traceA); + if (seq->traceG != NULL) free(seq->traceG); + if (seq->traceT != NULL) free(seq->traceT); + + if (seq->base != NULL) free(seq->base); + if (seq->basePos != NULL) free(seq->basePos); + + if (seq->edits != NULL) free(seq->edits); + if (seq->edBase != NULL) free(seq->edBase); + if (seq->edBasePos != NULL) free(seq->edBasePos); + + if (seq->info != NULL) free(seq->info); + + free(seq); +} + + + + +/* ---- General sequence information ---- */ + + + + +Boolean isDirty(Seq seq) +/* + Indicate if the sequence has been edited. +*/ +{ return(seq->dirty); +} + + + + +void setDirty(Seq seq, Boolean b) +/* + Set the sequence to the state of cleanliness indicated by `b'. +*/ +{ seq->dirty = b; +} + +/* isBottom and setBottom were added by lfw when the new element +was added to seq. seq->bottom */ + + +Boolean isBottom(Seq seq) +/* + Indicate if the sequence we're working with is bottom strand +*/ +{ return(seq->bottom); +} + + + + +void setBottom(Seq seq, Boolean b) +/* + Set the sequence to the state bottom if b is true and + top if b is false +*/ +{ seq->bottom = b; +} + + + + +Boolean isBasesOnly(Seq seq) +/* + Indicate if the sequence only contains bases, or if it + contains bases and traces. +*/ +{ return((seq->mode)==BasesOnly); +} + + + + +/* ---- Trace information ---- */ + + + + +int getNPoints(Seq seq) +/* + Return the number of points in this sequence. +*/ +{ return(seq->NPoints); +} + + + + +void getTraces(Seq seq, char base, int p0, int pN, int *traces) +/* + For basetype `base' in the sequence `seq', return in consecutive + elements of `traces' (an array of int) the values at the points + between and including p0 to pN, where p0<=pN. +*/ +{ int p; + int i; + TRACE *t; + + switch (base) + { case 'C': t = seq->traceC; break; + case 'A': t = seq->traceA; break; + case 'G': t = seq->traceG; break; + case 'T': t = seq->traceT; break; + } + + p = p0; + i = 0; + while (p<=pN) + { traces[i] = t[p]; + p++; + i++; + } +} + + + + +TRACE getMaxTraceVal(Seq seq) +/* + Return the maximum value from anywhere in any of the traces + from the sequence `seq'. +*/ +{ return(seq->maxTraceVal); +} + + + + +/* ---- Base information ---- */ + + + + +int getNBases(Seq seq, WhichBases which) +/* + Return the number of bases in the `which' part of `seq' +*/ +{ return((which == OrigBases) ? seq->NorigBases : seq->NedBases); +} + + +void getCutoffs(Seq seq, int *leftCutoff, int *rightCutoff) +/* + Return the number of characters to be cut off at either end. +*/ +{ *leftCutoff = seq->leftCutoff; + *rightCutoff = seq->rightCutoff; +} + + + +char getBase(Seq seq, WhichBases which, int baseNum) +/* + Given `baseNum' as the number of a base in the `which' part of + the sequence `seq', return the base found there. +*/ +{ return((which == OrigBases) + ? (seq->base[baseNum]) + : (seq->edits[baseNum] >= 0) + ? seq->base[seq->edits[baseNum]] + : seq->edBase[-(seq->edits[baseNum])] + ); +} + + +int baseNumToPoint(Seq seq, WhichBases which, int baseNum) +/* + Given `baseNum' as the number of a base in `which' part of + sequence `seq', return its point location. NULLPoint is + returned if `baseNum' was invalid. +*/ +{ return((which == OrigBases) + + ? /* Original sequence */ + (baseNum<0 || baseNum>seq->NorigBases-1) + ? NULLPoint + : seq->basePos[baseNum] + + : /* Edited sequence */ + (baseNum<0 || baseNum>seq->NedBases-1) + ? NULLPoint + : (seq->edits[baseNum] >= 0) + ? seq->basePos[seq->edits[baseNum]] + : seq->edBasePos[-(seq->edits[baseNum])] + ); + +} + + + + +int pointToBaseNum(Seq seq, WhichBases which, int point) +/* + Given `point' as a point in the `which' part of sequence `seq', + return the base number of the next base to the right (or on + the point), or NULLBaseNum if there isn't one. +*/ +{ int l,r,N,m; + + if (point<0 || point>seq->NPoints-1) return(NULLBaseNum); + + /* + Let's use a groovy binary search. + Roughly, we wish to find an m (0..NBases-1) such that: + baseNumToPoint(m]>=point & baseNumToPoint(m-1]=point + + Maintaining these two, we adjust l and r (using a binary + chop) such that the complete array is spanned by these + sections. + */ + + /* Start off with the two sections empty */ + N = (which == OrigBases) ? seq->NorigBases : seq->NedBases; + l=0; r=N; /* NotinLS & MaybeInRS */ + + while (l=point + */ + if (r==N) + return(NULLBaseNum); + else + return(r); +} + + + + +/* ---- Base editing ---- */ + + + + +Boolean deleteBase(Seq seq, int baseNum) +/* + Delete the base `baseNum' from the sequence `seq'. +*/ +{ int i; + + /* Fail if base number bad */ + if ((baseNum<0) || (baseNum>(seq->NedBases-1))) return(False); + + /* Fail if in a cutoff region */ + if ((baseNum <= seq->leftCutoff-1) || + (baseNum > seq->NedBases-1-seq->rightCutoff)) return(False); + + seq->dirty = True; + + /* If this was a base we added, then free its slot */ + if (seq->edits[baseNum]<0) + seq->edBasePos[-(seq->edits[baseNum])] = NULLPoint; + + /* Shuffle all the entries in `edits' down one. */ + for (i=baseNum+1;iNedBases;i++) + seq->edits[i-1] = seq->edits[i]; + + seq->NedBases--; + return(True); +} + + + + +Boolean insertBase(Seq seq, char base, int baseNum) +/* + Insert the base `base' to the right of `baseNum'. Its position + is halfway between its neighbours, except when it would be + replacing a deleted base from the original sequence, in which + case it is placed there. + + If `baseNum' equals -1 `base' is added to the head of the + sequence. +*/ +{ int i; + int prevBasePos, nextBasePos; + int origBaseNum, origBasePos; + + + /* Fail if bad base number */ + if ((baseNum<-1) || (baseNum>(seq->NedBases-1))) return(False); + + /* Fail if in a cutoff region */ + if ((baseNum < seq->leftCutoff-1) || + (baseNum > seq->NedBases-1-seq->rightCutoff)) return(False); + + seq->dirty = True; + + + /* + Before we alter anything, find what will become the + surrounding positions + */ + prevBasePos = (baseNum == -1) + ? -1 + : baseNumToPoint(seq, EdBases, baseNum); + nextBasePos = (baseNum == (seq->NedBases-1)) + ? seq->NPoints-1 + : baseNumToPoint(seq, EdBases, baseNum+1); + + + /* + Shuffle all the entries in `edits' up one + */ + for (i=seq->NedBases-1;i>baseNum;i--) + seq->edits[i+1] = seq->edits[i]; + seq->NedBases++; + + + /* + Look in the original sequence to see if there is a base + which lies between prevBasePos and nextBasePos. + */ + origBaseNum = pointToBaseNum(seq, OrigBases, prevBasePos+1); + origBasePos = baseNumToPoint(seq, OrigBases, origBaseNum); + + if ((origBaseNum!=NULLBaseNum) && + (prevBasePosedits[baseNum+1] = origBaseNum; + } + else + { int slot; + + /* We can consider this a replacement, at the same position */ + + /* Find a free slot in the newBasePos (and thus newBase) table */ + for (slot=1; + (slotedBasePos[slot]!=NULLPoint); + slot++) + if (slot==MaxEdits) /* All entries used */ return(False); + + /* Fill in the edBasePos and edBase entries */ + seq->edBasePos[slot] = origBasePos; + seq->edBase[slot] = base; + + /* Put in the (negative) reference to this slot */ + seq->edits[baseNum+1] = -slot; + } + } + else + { int slot; + + /* Find a free slot in the newBasePos (and thus newBase) table */ + for (slot=1;(slotedBasePos[slot]!=NULLPoint);slot++) + if (slot==MaxEdits) /* All entries used */ return(False); + + /* Fill in the edBasePos and edBase entries */ + seq->edBasePos[slot] = (prevBasePos + nextBasePos) / 2; + seq->edBase[slot] = base; + + /* Put in the (negative) reference to this slot */ + seq->edits[baseNum+1] = -slot; + } + + + return(True); +} + + + + +Boolean setCutoffs(Seq seq, int leftCutoff, int rightCutoff) +/* + Set the cutoffs to be the indicated number of characters from + either end of the sequence. The result indicates success. +*/ +{ if ((leftCutoff+rightCutoff) > seq->NedBases) + return(False); + else + { seq->leftCutoff = leftCutoff; + seq->rightCutoff = rightCutoff; + return(True); + } +} diff --git a/src/ted/seq.h b/src/ted/seq.h new file mode 100644 index 0000000..9892bba --- /dev/null +++ b/src/ted/seq.h @@ -0,0 +1,282 @@ +#ifndef _seq_h +#define _seq_h + + +/* + Title: seq + + File: seq.h + Purpose: Sequence data type + Last update: Tue Nov 10 1992 +*/ + + +/* + This module encodes the `Seq' sequence data structure. + + A `Seq' contains information about bases and traces which are layed + out along a single dimension of points. The number of points in a + paricular sequence is given by `getNPoints', and these are numbered + 0..getNPoints-1. At each point there are four trace readings, one + for each base. + + There are two sequences of bases: the `original', and the + `editable'. There have `getNorigBases' and `getNedBases' respectively, + which are numbered 0..N-1. Bases are represented by `char's. Every + base is located at a particular point. + + When written out, the list of bases is trimmed by a left and a right + cutoff, which indicate the number of bases to remove from either + end. Editing cannot be performed in the cutoff regions. + + The behaviour of these routines is undefined if given NULLSeq or + an undefined sequence. + + SD. Added information field to seq data structure + +*/ + + + + +/* ---- Includes ---- */ + +#include /* IMPORT: Widget */ + + + + + +/* ---- Private ---- */ + +#define MaxEdits 200 + + +typedef short int2; /* Two byte integers */ +typedef int int4; /* Four byte integers */ +typedef unsigned short TRACE; /* for trace heights */ + +#define MaxInt2 (32767) /* An estimate of the lower bound */ + + +typedef enum +{ BasesOnly, + BasesAndTraces +} Mode; + +typedef enum +{ + ABIFormat, + ALFFormat, + PlainFormat, + SCFFormat +} Format; + +typedef struct +{ Mode mode; + Boolean dirty; /* Have any changes been made? */ + Boolean bottom; /* are we looking at the top or + bottom strand? */ + Format format; + + int NPoints; /* No. of points of data */ + int NorigBases; /* No. of bases in the original sequence */ + int NedBases; /* No. of bases in the edited sequence */ + + /* Traces */ + TRACE *traceC; /* Array of length `NPoints' */ + TRACE *traceA; /* Array of length `NPoints' */ + TRACE *traceG; /* Array of length `NPoints' */ + TRACE *traceT; /* Array of length `NPoints' */ + TRACE maxTraceVal; /* The maximal value in any trace */ + + /* Original bases */ + char *base; /* Array of length `NorigBases' */ + int2 *basePos; /* Array of length `NorigBases' */ + + /* Edited bases */ + int2 *edits; /* Array of length `NorigBases+MaxEdits' */ + char *edBase; /* Array of length `MaxEdits' */ + int2 *edBasePos; /* Array of length `MaxEdits' */ + + /* Cutoffs */ + int leftCutoff; /* Number of unwanted bases */ + int rightCutoff; /* Number of unwanted bases */ + + /* Miscellaneous Sequence Information */ + char *info; /* misc seq info */ + +} SeqData, *Seq; + + +extern Seq newSeq(Mode mode, int2 numPoints, int2 numBases); +/* + Allocate a new sequence, with the given sizes. + The sequence is initially clean with no bases cutoff. + If `mode' is BasesOnly, `numPoints' is ignored. + Returns NULLSeq on failure. +*/ + + + + +/* ---- Exports ---- */ + + +#define NULLSeq ((Seq)NULL) +#define NULLBaseNum (-1) +#define NULLPoint (-1) + + +typedef enum +{ OrigBases, + EdBases +} WhichBases; + + + + +/* ---- General sequence manipulation ---- */ + + +extern void freeSeq(Seq seq); +/* + Free a sequence. + Does nothing if given NULLSeq. +*/ + + +/* ---- General sequence information ---- */ + + +extern Boolean isDirty(Seq seq); +/* + Indicate if the sequence has been edited since last set clean. +*/ + + +extern void setDirty(Seq seq, Boolean b); +/* + Set the sequence to the state of cleanliness indicated by `b'. +*/ + +extern Boolean isBottom(Seq seq); +/* + Indicate if we're looking at bottom strand +*/ + + +extern void setBottom(Seq seq, Boolean b); +/* + Set the sequence to b - true if bottom, false if top strand +*/ + + +extern Boolean isBasesOnly(Seq seq); +/* + Indicate if the sequence only contains bases, or if it + contains bases and traces. +*/ + + +/* ---- Trace information ---- */ + + +extern int getNPoints(Seq seq); +/* + Return the number of points in this sequence. +*/ + + +extern void getTraces(Seq seq, char base, int p0, int pN, int *traces); +/* + For basetype `base' in the sequence `seq', return in consecutive + elements of `traces' (an array of int) the values at the points + between and including p0 to pN, where p0<=pN. +*/ + + +extern TRACE getMaxTraceVal(Seq seq); +/* + Return the maximum value from anywhere in any of the traces + from the sequence `seq'. +*/ + + +/* ---- Base information ---- */ + + +extern int getNBases(Seq seq, WhichBases which); +/* + Return the number of bases in the `which' part of `seq' +*/ + + +extern void getCutoffs(Seq seq, int *leftCutoff, int *rightCutoff); +/* + Return the number of characters to be cut off at either end. +*/ + + +extern char getBase(Seq seq, WhichBases which, int baseNum); +/* + Given `baseNum' as the number of a base in the `which' part of + the sequence `seq', return the base found there. +*/ + +/*extern int getBasePos(Seq seq, WhichBases which, int baseNum);*/ +/* + Given `baseNum' as the number of a base in the `which' part of + the sequence `seq', return the base position found there. +*/ + + + +extern int baseNumToPoint(Seq seq, WhichBases which, int baseNum); +/* + Given `baseNum' as the number of a base in `which' part of + sequence `seq', return its point location. NULLPoint is + returned if `baseNum' was invalid. +*/ + + +extern int pointToBaseNum(Seq seq, WhichBases which, int point); +/* + Given `point' as a point in the `which' part of sequence `seq', + return the base number of the next base to the right (or on + the point), or NULLBaseNum if there isn't one. +*/ + + +/* ---- Base editing ---- */ + + +extern Boolean deleteBase(Seq seq, int baseNum); +/* + Delete the base `baseNum' from the sequence `seq'. + The result indicates success. +*/ + + +extern Boolean insertBase(Seq seq, char base, int baseNum); +/* + Insert the base `base' to the rigt of `baseNum'. Its position + is halfway between its neighbours, except when it would be + replacing a deleted base from the original sequence, in which + case it is placed there. + + If `baseNum' equals -1 `base' is added to the head of the + sequence. + + The result indicates success. +*/ + + +extern Boolean setCutoffs(Seq seq, int leftCutoff, int rightCutoff); +/* + Set the cutoffs to be the indicated number of characters from + either end of the sequence. The result indicates success. +*/ + + +#endif /*_seq_h*/ diff --git a/src/ted/seqIOABI.c b/src/ted/seqIOABI.c new file mode 100644 index 0000000..76a3d6e --- /dev/null +++ b/src/ted/seqIOABI.c @@ -0,0 +1,427 @@ +/* + Title: seqIOABI + + File: seqIOABI.c + Purpose: IO of ABI sequences + Last update: Tue Nov 10 1992 + + Change log: + 27/11/90 SD writeSeqABI() outputs header to sequence file: + format: ;{noOfBases}{leftCutOff}{basesWritten}{type}{tra + cefile} + eg: ; 867 45 383ABI a09b7.s1RES + 28.11.90 SD put undesirables under STLOUIS compilation flag + 11.12.90 SD new static function tail to find file name in path name + 02.01.91 SD Merged with St.L version + 15.01.91 SD New include added (opp.h) + 30.07.91 SD Those ole FWO_ field blues + 17.09.91 LFW changed STLOUIS compilation flag to SAVE_EDITS + and AUTO_CLIP + 25.10.91 SD Machine independant I/O...removed BIGENDIAN flag + 10.11.92 SD FWO_ and S/N% interpretation. Comments for information + window. + + */ + + +/* + This module should be regarded as part of `seq' since it is + privy to the internal structure of `Seq'. + + At the moment, the `maxTraceVal' of the sequence is hardwired + as 1200, but I suspect it is encoded somewhere in the ABI file + structure, or if not, we should calculate it as we read the + traces in. + + The ABI results file is controlled by an index found towards + the end --- this is pointed to by a longword found at `IndexPO'. + The index consists of a number of entries, each of which is + four character label followed by 6 long words. The first of these + long words holds a simple count (starting at 1) for those cases + where there are multiple entries with the same label. Entries should + be found by label (and count), rather than their index position, + because entries can be ommited or new ones added. This happens when + ABI changes the version of their software and also depending + on whether the data was analysed or unalaysed. We do, however, + make assumptions about the relative order of entries. + + Ideally we would have a separate module which provides a number + of functions to extract the data we are interested in, keeping + the ABI format well wrapped up and out of harms way. + + Note that we are relying on the endian-ness of the machine being + appropriate so we can just read long words in as integers. This + should be recoded to deal with running on different endians. + + All references to the seq->bottom, the bottom strand of the + sequence, were added by lfw + */ + + + + +/* ---- Imports ---- */ + +#include "seq.h" /* IMPORT: Seq, BasesAndTraces, NULLSeq, + newSeq, freeSeq */ +#include "seqIOABI.h" +#include "seqIOEdit.h" /* IMPORT: writeEdSeq, + readEdSEq */ + +#include "fpoint.h" /* IMPORT: int_to_float */ +#include +#include /* IMPORT: fopen, fclose, fseek, ftell, fgetc, + EOF */ +#include + +#include "mach-io.h" /* IMPORT: machine independant io library */ +#include "dialogues.h" + +/* ---- Constants ---- */ + +#define BasesPerLine 50 /* For output formatting */ + + +/* + The index is located towards the end of the ABI trace file. + It's location is given by a longword at a fixed place. + */ +#define IndexPO ((off_t)26) + +#define IndexEntryLength 28 + + +/* + Here are some labels we will be looking for, four chars packed + into a long word. + */ +#define DataEntryLabel ((int_4) ((((('D'<<8)+'A')<<8)+'T')<<8)+'A') +#define BaseEntryLabel ((int_4) ((((('P'<<8)+'B')<<8)+'A')<<8)+'S') +#define BasePosEntryLabel ((int_4) ((((('P'<<8)+'L')<<8)+'O')<<8)+'C') +#define SpacingEntryLabel ((int_4) ((((('S'<<8)+'P')<<8)+'A')<<8)+'C') +#define SignalEntryLabel ((int_4) ((((('S'<<8)+'/')<<8)+'N')<<8)+'%') +#define FWO_Label ((int_4) ((((('F'<<8)+'W')<<8)+'O')<<8)+'_') +#define MCHNLabel ((int_4) ((((('M'<<8)+'C')<<8)+'H')<<8)+'N') +#define PDMFLabel ((int_4) ((((('P'<<8)+'D')<<8)+'M')<<8)+'F') +#define SMPLLabel ((int_4) ((((('S'<<8)+'M')<<8)+'P')<<8)+'L') +#define PPOSLabel ((int_4) ((((('P'<<8)+'P')<<8)+'O')<<8)+'S') + + + + +/* ---- Internal functions ---- */ + + + +static Boolean getIndexEntryLW(FILE *fp, off_t indexO, + uint_4 label, uint_4 count, int lw, + uint_4 *val) +/* + From the ABI results file connected to `fp' whose index starts + at byte offset `indexO', return in `val' the `lw'th long word + from the `count'th entry labelled `label'. + The result indicates success. + */ +{ off_t entryNum=-1; + int i; + uint_4 entryLabel, entryLw1; + + do + { entryNum++; + if (fseek(fp, indexO+(entryNum*IndexEntryLength), 0) != 0) return(-1); + if (!be_read_int_4(fp, &entryLabel)) return(False); + if (!be_read_int_4(fp, &entryLw1)) return(False); + } while (!(entryLabel == label && entryLw1 == count)); + + for(i=2; i<=lw; i++) + if (!be_read_int_4(fp, val)) return(-1); + + return(True); +} + + + + +/* ---- Exports ---- */ + + + + +Seq readSeqABI(char *fn) +/* + Read the ABI format sequence with name `fn' into `seq'. + All printing characters (as defined by ANSII C `isprint') + are accepted, but `N's are translated to `-'s. If bottom + is True, we read the sequence in in reverse and complemented + A NULL result indicates failure. + + In this module all references to the bottom strand were + inserted by lfw. + */ +{ Seq seq = NULLSeq; + uint_4 fwo_; /* base -> lane mapping */ + FILE *fp; + uint_4 numPoints, numBases; + uint_4 signalO; + + uint_4 indexO; /* File offset where the index is */ + uint_4 baseO; /* File offset where the bases are stored */ + uint_4 basePosO; /* File offset where the base positions are stored */ + uint_4 dataCO; /* File offset where the C trace is stored */ + uint_4 dataAO; /* File offset where the A trace is stored */ + uint_4 dataGO; /* File offset where the G trace is stored */ + uint_4 dataTO; /* File offset where the T trace is stored */ + uint_4 MCHN_O; /* File offset where the machine name is stored */ + uint_4 PDMF_O; /* File offset where the dye primer guff is stored */ + /* + uint_4 SMPL_O; /. File offset where the sample name is stored ./ + */ + + int i; + + + if ((fp = fopen(fn, "rb")) == NULL) return(NULLSeq); + + + /* Get the index offset */ + if ((fseek(fp, IndexPO, 0) != 0) || (!be_read_int_4(fp, &indexO))) + { fclose(fp); + return(NULLSeq); + } + + /* Get the number of points */ + if (!getIndexEntryLW(fp,(off_t)indexO,DataEntryLabel,9,3,&numPoints)) + { fclose(fp); + return(NULLSeq); + } + + /* Get the number of bases */ + if (!getIndexEntryLW(fp,(off_t)indexO,BaseEntryLabel,1,3,&numBases)) + { fclose(fp); + return(NULLSeq); + } + + /* Allocate the sequence */ + if ((seq = newSeq(BasesAndTraces, numPoints, numBases)) == NULLSeq) + { fclose(fp); + return(NULLSeq); + } + + seq->mode = BasesAndTraces; + seq->format = ABIFormat; + seq->dirty = False; + seq->maxTraceVal = 1200; + seq->NPoints = numPoints; + seq->NorigBases = numBases; + seq->NedBases = numBases; + seq->bottom = False; + + + /************************************************************* + * + *************************************************************/ + + /* + ** The order of the DATA fields is determined by the field FWO_ + ** Juggle around with data pointers to get it right + */ + { + uint_4 *dataxO[4]; + + dataxO[0] = &dataCO; + dataxO[1] = &dataAO; + dataxO[2] = &dataGO; + dataxO[3] = &dataTO; + + /* Get the Freak World Out (FWO?) field ... */ + if (!getIndexEntryLW(fp,(off_t)indexO,FWO_Label,1,5,&fwo_)) + { fclose(fp); + return(NULLSeq); + } +#define baseIndex(B) ((B)=='C'?0:(B)=='A'?1:(B)=='G'?2:3) + /*Get the positions of the four traces */ + if (! ( getIndexEntryLW(fp,(off_t)indexO,DataEntryLabel,9 ,5,dataxO[baseIndex((char)(fwo_>>24&255))]) && + getIndexEntryLW(fp,(off_t)indexO,DataEntryLabel,10,5,dataxO[baseIndex((char)(fwo_>>16&255))]) && + getIndexEntryLW(fp,(off_t)indexO,DataEntryLabel,11,5,dataxO[baseIndex((char)(fwo_>>8&255))]) && + getIndexEntryLW(fp,(off_t)indexO,DataEntryLabel,12,5,dataxO[baseIndex((char)(fwo_&255))]) + ) + ) + { fclose(fp); + freeSeq(seq); + return(NULLSeq); + } + + } + + + /* Read in the C trace */ + if (fseek(fp, (off_t)dataCO, 0) != 0) {fclose(fp);freeSeq(seq);return(NULLSeq);} + for (i=0;i<(seq->NPoints);i++) + { if (!be_read_int_2(fp, &(seq->traceC[i]))) + {fclose(fp);freeSeq(seq);return(NULLSeq);} + } + + /* Read in the A trace */ + if (fseek(fp, (off_t)dataAO, 0) != 0) {fclose(fp);freeSeq(seq);return(NULLSeq);} + for (i=0;i<(seq->NPoints);i++) + { if (!be_read_int_2(fp, &seq->traceA[i])) + {fclose(fp);freeSeq(seq);return(NULLSeq);} + } + + /* Read in the G trace */ + if (fseek(fp, (off_t)dataGO, 0) != 0) {fclose(fp);freeSeq(seq);return(NULLSeq);} + for (i=0;i<(seq->NPoints);i++) + { if (!be_read_int_2(fp, &seq->traceG[i])) + {fclose(fp);freeSeq(seq);return(NULLSeq);} + } + + + /* Read in the T trace */ + if (fseek(fp, (off_t)dataTO, 0) != 0) {fclose(fp);freeSeq(seq);return(NULLSeq);} + for (i=0;i<(seq->NPoints);i++) + { if (!be_read_int_2(fp, &seq->traceT[i])) + {fclose(fp);freeSeq(seq);return(NULLSeq);} + } + + + + /* Read in the bases */ + if (! (getIndexEntryLW(fp,(off_t)indexO,BaseEntryLabel,1,5,&baseO) && + (fseek(fp, (off_t)baseO, 0) == 0) + ) + ) + { fclose(fp); + freeSeq(seq); + return(NULLSeq); + } + for (i=0;i<(seq->NorigBases);i++) + { int ch; + + if ((ch = fgetc(fp)) == EOF) + { fclose(fp); + freeSeq(seq); + return(NULLSeq); + } + seq->base[i] = (ch == 'N') ? '-' : ch; + } + + + + + /* Read in the base positions */ + if (!(getIndexEntryLW(fp,(off_t)indexO,BasePosEntryLabel,1,5,&basePosO) && + (fseek(fp, (off_t)basePosO, 0) == 0) + ) + ) + { fclose(fp); + freeSeq(seq); + return(NULLSeq); + } + + for (i=0;i<(seq->NorigBases);i++) + { if (!be_read_int_2(fp, (uint_2 *)&seq->basePos[i])) + { fclose(fp); freeSeq(seq); return(NULLSeq); } + } + + + /************************************************************* + * Gather useful information + *************************************************************/ + { + char comment[1024]; + char line[128]; + int4 spacing; + int4 ppos; + + *comment = '\0'; + + + /* Get Signal Strength Offset */ + if (getIndexEntryLW(fp,(off_t)indexO,SignalEntryLabel,1,5,&signalO)) { + int2 C,A,G,T; + int2 *base[4]; + base[0] = &C; + base[1] = &A; + base[2] = &G; + base[3] = &T; + if (fseek(fp, (off_t)signalO, 0) >= 0 && + be_read_int_2(fp, (uint_2 *)base[baseIndex((char)(fwo_>>24&255))]) && + be_read_int_2(fp, (uint_2 *)base[baseIndex((char)(fwo_>>16&255))]) && + be_read_int_2(fp, (uint_2 *)base[baseIndex((char)(fwo_>>8&255))]) && + be_read_int_2(fp, (uint_2 *)base[baseIndex((char)(fwo_&255))])) { + sprintf(line,"avg_signal_strength = C:%d A:%d G:%d T:%d\n",C,A,G,T); + strcat(comment,line); + } + } + + /* Get the spacing.. it's a float but don't worry yet */ + if (getIndexEntryLW(fp,(off_t)indexO,SpacingEntryLabel,1,5,(uint_4*)&spacing)) { + sprintf(line,"avg_spacing = %6.2f\n",int_to_float(spacing)); + strcat(comment,line); + } + + + /* Get primer position */ + if (getIndexEntryLW(fp,(off_t)indexO,PPOSLabel,1,5,(uint_4 *)&ppos)) { + sprintf(line,"primer_position = %d\n",(ppos>>16)); /* ppos stores in MBShort of pointer */ + strcat(comment,line); + } + + /* Get Machine Name Offset */ + if (getIndexEntryLW(fp,(off_t)indexO,MCHNLabel,1,5,&MCHN_O)) { + if (fseek(fp, (off_t)MCHN_O, 0) >= 0) { + unsigned char l; + char buffer[256]; + /* first byte is a length */ + fread(&l,sizeof(char),1,fp); + fread(buffer,l,1,fp); + sprintf(line,"machine_name = %.*s\n",l,buffer); + strcat(comment,line); + } + } + + /* Get Dye Primer Offset */ + if (getIndexEntryLW(fp,(off_t)indexO,PDMFLabel,1,5,&PDMF_O)) { + if (fseek(fp, (off_t)PDMF_O, 0) >= 0) { + unsigned char l; + char buffer[256]; + /* first byte is a length */ + fread(&l,sizeof(char),1,fp); + fread(buffer,l,1,fp); + sprintf(line,"dye_primer = %.*s\n",l,buffer); + strcat(comment,line); + } + } + + /* Get Sample Name Offset */ + /* + * The code here needs to be a bit more clever + * When sample name length is less than (or equal?) four, the + * string is packed into the SMPL_O field + if (getIndexEntryLW(fp,(off_t)indexO,SMPLLabel,1,5,&SMPL_O)) { + if (fseek(fp, (off_t)SMPL_O, 0) >= 0) { + unsigned char l; + char buffer[256]; + /. first byte is a length ./ + fread(&l,sizeof(char),1,fp); + fread(buffer,l,1,fp); + sprintf(line,"sample_name = %.*s\n",l,buffer); + strcat(comment,line); + } + } + */ + + + /* dumplicate string and set info */ + { + char *s = (char *)malloc(strlen(comment)+1); + strcpy(s,comment); + seq->info = s; + } + } + + /* Finished with the file */ + fclose(fp); + + return(seq); +} diff --git a/src/ted/seqIOABI.h b/src/ted/seqIOABI.h new file mode 100644 index 0000000..6e9aeca --- /dev/null +++ b/src/ted/seqIOABI.h @@ -0,0 +1,39 @@ +#ifndef _seqIOABI_h +#define _seqIOABI_h + + +/* + Title: seqIOABI + + File: seqIOABI.h + Purpose: IO of ABI sequences + Last update: Mon May 28 1990 +*/ + + + + +/* ---- Imports ---- */ + + +#include "seq.h" /* IMPORT: Seq */ + +#include /* IMPORT: Boolean */ + + + + +/* ---- Exports ---- */ + + +extern Seq readSeqABI(char *fn); +/* + Read the ABI format sequence with name `fn' into `seq'. + All printing characters (as defined by ANSII C `isprint') + are accepted, but `N's are translated to `-'s. + A NULLSeq result indicates failure. + + enzString is the enzyme string that is to be used for + searching for the left cutoff of sequence +*/ +#endif /*_seqIOABI_h*/ diff --git a/src/ted/seqIOALF.c b/src/ted/seqIOALF.c new file mode 100644 index 0000000..61735fc --- /dev/null +++ b/src/ted/seqIOALF.c @@ -0,0 +1,334 @@ +/* + Title: seqIOALF + + File: seqIOALF.c + Purpose: IO of ALF sequences + Last update: Tue Nov 10 1992 + */ + +/* + Change Log :- + 14.01.91 SD + when complimenting the sequence with an odd number of bases, + the middle base position was not adjusted. + 15.01.91 SD Put StLouis stuff on compilation flag + 15.01.91 SD New include file (opp.h) + 02.08.91 SD Changes the mapping of uncertainty codes so that we + now only generate A C G T and - + Previously... bug in interpreting ALF integer fields. + We now treat them as unsigned. + 17.09.91 LFW changed STLOUIS compilation flag to SAVE_EDITS + and AUTO_CLIP + 25.10.91 SD Machine independant I/O...removed BIGENDIAN flag + 25.11.91 SD There was a hard limit (of 1024) for allocation of + space for number of bases, yet program would + read in more if there were any, causing nasties to happen. + + */ + +/* RMD I made substantial changes to this file 12/28/90 so as to + read sequence data more freely (necessary when reading data from + multiple trace files). + The affected area is indicated by comments starting RMD, like + this one. + */ + +/* This file was adapted by LFW from seqIOABI.c. + + At the moment, the `maxTraceVal' of the sequence is hardwired + as 1200. This fudge worked for the ABI, but is not really good + for the ALF. To keep compatibility we keep 1200 as a max and + scale to fit (search for scaleFactor). This needs work. + + The ALF results file is a concatenation of many files with an + index structure at the beginning, consisting of a 512 byte + block that we ignore, followed by 128 byte blocks describing + each file. All files, including the header region, are rounded + up to a multiple of 512 bytes long. + The getIndexEntry routines identify the 128 byte index component + of interest by matching 4 chars of its ASCII label, then extract + the field of choice from that entry. + + Note that the SUN and PC are of opposite endian-ness, so that + we have to provide special routines to read words and longwords + from the results file. Luckily the floating point numbers are + written out in ASCII. + + All references to the seq->bottom, the bottom strand of the + sequence, were added by lfw. + */ + + +/* ---- Imports ---- */ + +#include "seq.h" /* IMPORT: Seq, BasesAndTraces, NULLSeq, + newSeq, freeSeq */ +/*#include "seqIOABI.h"*/ +#include "seqIOEdit.h" /* IMPORT: writeEdSeq, + readEdSEq */ + + +#include +#include /* IMPORT: fopen, fclose, fseek, ftell, fgetc, + EOF */ +#include "mach-io.h" + +/* ---- Constants ---- */ + +#define BasesPerLine 50 /* For output formatting */ + +#define IndexEntryLength ((off_t)128) + + +/* + Here are some labels we will be looking for, four chars packed + into a long word. + */ +#define EntryLabel ((uint_4) ((((('A'<<8)+'L')<<8)+'F')<<8)+' ') +#define BaseEntryLabel ((uint_4) ((((('S'<<8)+'e')<<8)+'q')<<8)+'u') +#define DataEntryLabel ((uint_4) ((((('P'<<8)+'r')<<8)+'o')<<8)+'c') + + +/* ---- Internal functions ---- */ + +static Boolean getIndexEntryLW(FILE *fp, off_t indexO, + uint_4 label, int lw, + uint_4 *val) +/* + From the ALF results file connected to `fp' whose index starts + at byte offset `indexO', return in `val' the `lw'th long word + from the entry labelled `label'. + The result indicates success. + */ +{ off_t entryNum=-1; + int i; + uint_4 entryLabel; + + do + { entryNum++; + if (fseek(fp, indexO+(entryNum*IndexEntryLength), 0) != 0) + return(-1); + + if (!be_read_int_4(fp, &entryLabel)) return(False); + } while (!(entryLabel == label)); + + for(i=2; imode = BasesAndTraces; + seq->format = ALFFormat; + seq->dirty = False; + seq->maxTraceVal = 0; + seq->NPoints = numPoints; + seq->bottom = False; + + /* RMD set ->NorigBases and ->NedBases after reading them in */ + + /* read in the sequence */ + + if (!(fseek(fp, (off_t)baseO, 0) == 0)) + goto abort ; + + { /* new locals introduced by LFW and/or RMD for the ALF */ + int numBases; /* number of nucleotides read in */ + float bp ; + char ch; + + + for (numBases = 0 ; ftell(fp) < baseO+(unsigned short)actBaseDataSize && numBasesbase[numBases] = ch; + seq->basePos[numBases] = bp; + ++numBases ; + + } + } + + seq->NorigBases = numBases; + seq->NedBases = numBases; + } + + /* read in the traces , stored in 2 byte integers in records in the + order A C G T A C G T A C G T ...*/ + + if (fseek(fp, (off_t)(dataO+header_size), 0) != 0) + goto abort ; + + num_points = 0; + + for (i=0;i<(seq->NPoints);i++) + { if (!le_read_int_2(fp, &(seq->traceA[i]))) + {fclose(fp);freeSeq(seq);return(NULLSeq);} + if (seq->maxTraceVal < seq->traceA[i]) seq->maxTraceVal = seq->traceA[i]; + if (!le_read_int_2(fp, &(seq->traceC[i]))) + {fclose(fp);freeSeq(seq);return(NULLSeq);} + if (seq->maxTraceVal < seq->traceC[i]) seq->maxTraceVal = seq->traceC[i]; + if (!le_read_int_2(fp, &(seq->traceG[i]))) + {fclose(fp);freeSeq(seq);return(NULLSeq);} + if (seq->maxTraceVal < seq->traceG[i]) seq->maxTraceVal = seq->traceG[i]; + if (!le_read_int_2(fp, &(seq->traceT[i]))) + {fclose(fp);freeSeq(seq);return(NULLSeq);} + if (seq->maxTraceVal < seq->traceT[i]) seq->maxTraceVal = seq->traceT[i]; + + if (seq->traceA[i]==0 && seq->traceT[i]==0 && seq->traceC[i]==0 && seq->traceG[i]==0 && i > (numPoints-64)) break; + + num_points++; + } + + + /* Finished with the file */ + fclose(fp); + + return(seq); + + abort: + fclose(fp); + freeSeq(seq); + return(NULLSeq); +} diff --git a/src/ted/seqIOALF.h b/src/ted/seqIOALF.h new file mode 100644 index 0000000..28b7388 --- /dev/null +++ b/src/ted/seqIOALF.h @@ -0,0 +1,40 @@ +#ifndef _seqIOALF_h +#define _seqIOALF_h + + +/* + Title: seqIOALF + + File: seqIOALF.h + Purpose: IO of ABI sequences + Last update: Tues Dec 11 1990 +*/ + + + + +/* ---- Imports ---- */ + + +#include "seq.h" /* IMPORT: Seq */ + +#include /* IMPORT: Boolean */ + + + + +/* ---- Exports ---- */ + + +extern Seq readSeqALF(char *fn); +/* + Read the ABI format sequence with name `fn' into `seq'. + All printing characters (as defined by ANSII C `isprint') + are accepted, but `N's are translated to `-'s. + A NULLSeq result indicates failure. + + enzString is the enzyme string that is to be used for + searching for the left cutoff of sequence +*/ + +#endif /*_seqIOALF_h*/ diff --git a/src/ted/seqIOEdit.c b/src/ted/seqIOEdit.c new file mode 100644 index 0000000..b248555 --- /dev/null +++ b/src/ted/seqIOEdit.c @@ -0,0 +1,770 @@ +/* + Title: seqIOEdit + + File: seqIOEdit.c + Purpose: IO of the editted portion of plain or edited sequences + + Last update: Monday 24 February 1992 + + Change log :- + 15.01.91 SD New include file (opp.h) + 04.12.91 lfw added sample lanes up to 40 instead of 24 + 04.12.91 changed the way the left cutoff is found...now I allow + look for first occurrence of the left cutting sequence; + looking first for an exact match, then a match with + one mismatch, then with two...if nothing is found at + that point I assume the left cutoff is not there + 24.02.92 SD Fixed bug in findRightCutoff() when checking for overlap with + leftCutoff. There was some confusion over what the value of + rightCutoff actually means. + 18.11.92 lfw changed the names of the temporary files to .abc* instead + of abc*, also used the remove() command rather than system() to get + rid of the files and added another remove call so they will be sure to + be removed + + + */ + + +/* ---- Imports ---- */ +#include +#include "seqIOEdit.h" + +#include "seq.h"/* IMPORT: Seq, BasesAndTraces, NULLSeq, + newSeq, freeSeq */ +#include "opp.h" /* IMPORT: oppInitialise */ +#include "dialogues.h" + + +extern int findPercntAmbig(char *theSeq, int num_bases); + +/* ---- Internal Functions ---- */ + +int findPercntAmbig(char *theSeq, int num_bases); + +static void text_to_output(char *vec,int stp,int endp,int dvice,char *outfile) + +/* + * text_to_output(vec,stp,endp,dvice,outfile) + * input: char **vec,*outfile; int stp, endp, dvice; + * this program output a specified portion of a genbank file + * (from vec[stp] to vec[endp]) to the screen (default), a file (dvice + * =1), or lpr (dvice =2). Outfile is the input filename if you wish to + * output the information to a specified file. stp and endp are integers, + * not pointers. + */ + +{ + int i; + FILE *fopen(),*fp,*where; + + + if (dvice == 1) { + if ((fp = fopen(outfile,"a"))==NULL) { + printf ("\nERROR: can't open file %s\n",outfile); + return; + } + else where = fp; + } + else if (dvice == 2) { + if ((fp = fopen("junkfile.","a"))==NULL) { + printf ("\nERROR: can't open file junkfile. to output to the lpr\n"); + return; + } + else where = fp; + } + else { + /* default : */ + where = stdout; + } + + for (i = stp; i < endp; i++) + putc(vec[i],where); + + if ((dvice == 1) || (dvice == 2)) fclose(fp); + +} + +int checkForExistingEdFile(char *fn) +/* + * check to see if there is an existing .seq.n file; + * return the largest n or 0 if no files existed, + * return a -1 if it was a problem with opening files + */ + +{ + char vec[500]; + int last_ed_num; + FILE *fp; + int i; + + /* make sure there are no files with the names I'm about to use*/ + remove (".abcxyztmpsh."); + remove (".abcxyztmpout."); + + + /* write a little shell to see the last n in your_filename.n + in the current directory */ + if ((fp=fopen(".abcxyztmpsh.", "w")) == NULL) return(-1); + fclose(fp); + sprintf(vec,"for i in 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 \n"); + i = strlen(vec); + sprintf(vec+i," do\n oldname=%s.$i\n if test -f $oldname\n then echo $i\n",fn); + i = strlen(vec); + sprintf(vec+i," fi\ndone\n"); + + text_to_output(vec,0,strlen(vec),1,".abcxyztmpsh."); + + /* execute the shell and have it output the last number it found into + a file called abcxyztmpout. */ + system("sh .abcxyztmpsh. | tail -1 > .abcxyztmpout."); + + /* read that number from that file */ + + if ((fp=fopen(".abcxyztmpout.", "r")) == NULL) return(-1); + vec[0]=getc(fp); + fclose(fp); + /* if that number was EOF return(0), nothing found */ + if (vec[0]==EOF) { + remove (".abcxyztmpsh."); + remove (".abcxyztmpout."); + + return(0); + } + + vec[1]='\0'; + + sscanf(vec,"%d",&last_ed_num); + remove (".abcxyztmpsh."); + remove (".abcxyztmpout."); + + return(last_ed_num); +} + +Boolean isDotSeq(char *fn) +/* make sure there is a .seq on the end of fn, puts one on + if there is not */ +{ + int i; + + i = strlen(fn)-1; + if (fn[i]!='q' || fn[i-1]!='e' || fn[i-2]!='s' || fn[i-3]!='.') + return(False); + else return(True); +} + +void stripDotSeq(char *fn) +/* there is a .seq on the end of fn, strip it off */ + +{ int i; + + i = strlen(fn)-1; + + if (fn[i]=='q' && fn[i-1]=='e' && fn[i-2]=='s' && fn[i-3]=='.') + fn[i-3]='\0'; + + return; +} + +void stripDotNum(char *fn) +/* if there is a .num on the end of fn, strip it off */ + +{ int i; + + i = strlen(fn)-1; + + if (isdigit(fn[i]) && fn[i-1]=='.') + fn[i-1]='\0'; + else if (isdigit(fn[i]) && isdigit(fn[i-1]) && fn[i-2]=='.') + fn[i-2]='\0'; + + return; +} + +int isDotNum(char *fn) +/* + * checks if there is a .1 or .2 or .m on the inputfilename. + * If there is, then returns that num. If not returns -1. + */ +{ + int i,j; + int dotnum; + char *atemp; + + atemp = (char *)calloc(20,sizeof(char)); + + i = strlen(fn)-1; + j = 0; + + if (isdigit(fn[i]) && (fn[i-1]=='.')) { + atemp[0] = fn[i]; + atemp[1] = fn[i+1]; + sscanf(atemp,"%d",&dotnum); + free(atemp); + return(dotnum); + } + else if (isdigit(fn[i]) && isdigit(fn[i-1]) && fn[i-2]=='.') { + atemp[0] = fn[i-1]; + atemp[1] = fn[i]; + atemp[2] = '\0'; + sscanf(atemp,"%d",&dotnum); + free(atemp); + return(dotnum); + } + + free(atemp); + return(-1); +} + +static void get_compl_seq(char *ac_seq,char *aseq,int stp,int endp, int seq_len, int rev) + +/* + * uses aseq to find the sequence that + * would appear on the opposite strand and places + * that sequence in ac_seq; stp is the starting point on + * aseq and endp is the ending point on aseq; + * seq_len is the length of the input sequence, + * if rev==1 reverses as well as complements, if rev==0 + * only complements + */ +{ + int i; + + oppInitialize(); + + if (rev == 1) { + for (i = stp; i <= endp; i++) + ac_seq[seq_len - i] = opp[aseq[i]]; + } + else { + for (i = stp; i <= endp; i++) + ac_seq[i] = opp[aseq[i]]; + ac_seq[i-1] ='\0'; + } + + + return; +} + + +/* ---- Externals ---- */ + +Boolean writeEdSeq(Seq seq, char *fn) + +{ + FILE *fp; + int i,j; + char ed_fn[200]; + int last_ed_num; /* largest n of fn.seq.n in + current directory */ + + oppInitialize(); + + /* make sure the filename does not have a .seq on the end */ + stripDotSeq(fn); + + /* get the n to put on inputfilename.n for the edited + file to be kept */ + last_ed_num = checkForExistingEdFile(fn); + + if (last_ed_num == -1) return(False); + else sprintf(ed_fn,"%s.%d",fn,last_ed_num+1); + /* that line takes care of 0 too, because + it names the file fn.seq.1 */ + + /* Open for writing, text */ + if ((fp=fopen(ed_fn, "w")) == NULL) return(False); + + + /* write information in the following format: + NedBases*edits array*edBase array*edBasePos array* + each division ends in an * and within + each division entries are separated by spaces */ + + if (seq->bottom) { + fprintf(fp," %6d*%3d*%6d*%6d*",seq->NorigBases,seq->NedBases,seq->rightCutoff,seq->leftCutoff); + } + else { + fprintf(fp," %6d*%3d*%6d*%6d*",seq->NorigBases,seq->NedBases,seq->leftCutoff,seq->rightCutoff); + } + + /* print out edits array */ + + if (seq->bottom) { + for (i=seq->NorigBases+MaxEdits-1; + i > -1; + i--) + { + j = 0; + if (i==seq->NorigBases+MaxEdits-1) + /* this part is a fudge to stick in 0 0 */ + fprintf(fp,"%6d %6d ",j,j); + + + if (((seq->edits[i]!=0) && ((seq->NedBases -1- i)>=0)) || i==0){ + if (seq->edits[i] <0) + fprintf(fp,"%6d %6d ",seq->NedBases -1- i,seq->edits[i]); + else + fprintf(fp,"%6d %6d ",seq->NedBases -1 -i,seq->NorigBases - 1-(seq->edits[i])); + } + } + } + else { + for (i=0; + iNorigBases+MaxEdits; + i++) { + + if ((seq->edits[i]!=0) || (i==0)) + fprintf(fp,"%6d %6d ",i,seq->edits[i]); + } + } + + fprintf(fp,"%6d %6d ",NULLPoint,NULLPoint); + + fprintf(fp,"*"); + + /* print out non-NULL entries in edBase array */ + for (i=1; + iedBase[i] == NULL) { + fprintf(fp,"* "); + break; + } + else { + if (seq->bottom) + fprintf(fp,"%c ",opp[seq->edBase[i]]); + else + fprintf(fp,"%c ",seq->edBase[i]); + } + } + + fprintf(fp,"*"); + + /* print out non-NULL entries in edBasePos array */ + + for (i=1; + iedBasePos[i] == NULLPoint) { + fprintf(fp,"%6d ",NULLPoint); /* -1 is NULLPoint*/ + break; + } + else { + int fudge; + /* + * when you're plotting the strand in the reverse + * order, you must move the starting position over by + * the width of one character. Because positions in the + * other file, already take into account the character + * width. Therefore, fudge = ~character width + basePos + * of the first base + */ + + fudge = seq->basePos[0] + 6; + + if (seq->bottom) { + /* fprintf(fp,"%6d ",seq->basePos[seq->NorigBases-1]-seq->edBasePos[i]+fudge); */ + fprintf(fp,"%6d ",seq->NPoints-seq->edBasePos[i]); + } + else + fprintf(fp,"%6d ",seq->edBasePos[i]); + } + } + + fprintf(fp,"*"); + (void) fclose(fp); + return(True); +} + + + +Boolean readEdSeq(Seq seq, char *fn, int dotnum) +/* + * reads in the most recent fn.seq.n file. This + * file should be of the form: + * basePos;edBasePos;edBase + * returns True if it read in the sequence from + * an editted file, and False if there was no + * editted file or if there was a problem + * + * dotnum == -1 if the user did not specify a + * version number of the sequence to read in, + * if they want version m read in. + */ + +{ + int last_ed_num; + char ed_fn[200]; + FILE *fp; + + oppInitialize(); + + /* make sure the filename does not have a .seq on the end */ + stripDotSeq(fn); + + if (dotnum == -1) { + /* get the n to put on fn.seq.n */ + last_ed_num = checkForExistingEdFile(fn); + if (last_ed_num == -1) return(False); + else if (last_ed_num == 0) return(False); + else sprintf(ed_fn,"%s.%d",fn,last_ed_num); + } + else + sprintf(ed_fn,"%s.%d",fn,dotnum); + + + /* Open for reading, text */ + if ((fp=fopen(ed_fn, "r")) == NULL) return(False); + fclose(fp); + + if (processEdSeqFile(seq,ed_fn)) + return(True); + else return(False); +} + +Boolean processEdSeqFile(Seq seq, char *fn) + +/* + * processes a char vector containing the Editted + * sequence in the following format: + * + * NedBases*NorigBases*leftCutoff*rightCutoff*negative + * components of edits array in the form (position, + * negative number)*non NULL components edBase array* + * non NULL components edBasePos array* + * + * each division ends in an H. The end of the + * edits array is signaled by a -1 -1 entry. Within + * each division entries are separated by spaces + * + * sticks the information it finds into the seq array + * + * returns false if there was a problem with the file format + */ +{ + FILE *fp; + int nbases; + char achar; + int i,j,k; + char ed_fn[200]; + + strcpy(ed_fn,fn); + + if ((fp=fopen(ed_fn, "r")) == NULL) return(False); + + fscanf(fp," %6d",&nbases); + achar = getc(fp); + if (achar != '*') { + printf("ERROR: Input editted sequence was of wrong format \n(No asterisk was found after the number of bases)\n"); + fclose(fp); + return(False); + } + + + fscanf(fp,"%3d",&i); + achar = getc(fp); + if (achar != '*') { + printf("ERROR: Input editted sequence was of wrong format \n(No asterisk was found after the number of edited bases)\n"); + fclose(fp); + return(False); + } + + seq->NedBases = i; + if (nbases != seq->NorigBases) { + printf("ERROR: Input editted sequence was of wrong format\n (Number of editted bases has changed)\n"); + fclose(fp); + return(False); + } + + + fscanf(fp,"%6d",&j); + achar = getc(fp); + if (achar != '*') { + printf("ERROR: Input editted sequence was of wrong format \n(No asterisk was found after the left cutoff)\n"); + fclose(fp); + return(False); + } + + fscanf(fp,"%6d",&k); + achar = getc(fp); + if (achar != '*') { + printf("ERROR: Input editted sequence was of wrong format \n(No asterisk was found after the right cutoff)\n"); + fclose(fp); + return(False); + } + + if (seq->bottom) { + seq->leftCutoff = k; + seq->rightCutoff = j; + } + else { + seq->leftCutoff = j; + seq->rightCutoff = k; + } + + /* read in the seq->edits array */ + + for (i=1; + iNorigBases+MaxEdits; + i++) + { + fscanf(fp,"%6d %6d ",&j,&k); + if (j==NULLPoint) break; + else { + if (seq->bottom) { + if (k<0) /* then it is a reference to the edBase array */ + seq->edits[seq->NedBases-1-j]=k; + else /*it is a base number which needs to be converted + to the base number on the opposite strand */ + seq->edits[seq->NedBases-1-j]=seq->NorigBases-1-k; + } + else + seq->edits[j]=k; + } + + } + achar = getc(fp); + if (achar != '*') { + printf("ERROR: Input editted sequence was of wrong format \n(Error in the edits array)\n"); + fclose(fp); + return(False); + } + + /* read in the seq->edBase array */ + for (i=1; + ibottom) + seq->edBase[i]=opp[achar]; + else + seq->edBase[i]=achar; + } + } + achar = getc(fp); + if (achar != '*') { + printf("Input editted sequence was of wrong format\n(Extraneous information after edBases and before the *\n"); + fclose(fp); + return(False); + } + + + /* read in the seq->edBasePos array */ + + for (i=1; + ibasePos[0] + 6; + + if (seq->bottom) { + /* seq->edBasePos[i]=seq->basePos[seq->NorigBases-1]-k+fudge;*/ + seq->edBasePos[i]=seq->NPoints - k; + } + else + seq->edBasePos[i]=k; + } + + } + achar = getc(fp); + if (achar != '*') { + printf("ERROR: Input editted sequence was of wrong format\n(Error in editted Base Position array)\n"); + fclose(fp); + return(False); + } + + /* + * Don't set the seq to Dirty, otherwise the user won't know + * if they have or have not edited their input edited sequence + */ + fclose(fp); + return(True); +} + + + + +int findLeftCutoff(Seq seq, char *enzInString) +/* + * looks for left cutoff, if it doesn't find a "enzInString", then + * it looks from enzInString less it's last character, etc + */ + +{ + int maxStartPos=100; /* if the enzyme site wasn't found before this + baseNum, then that's probably not the cloning + site */ + int i,j,found; + /* int jj,kk; */ + int indices[100]; + int num_matches; + char *theSeq; + int num_bases; + char enzString[100]; + /* char enztemp[100]; */ + + found = 0; + + if (seq->bottom) + get_compl_seq(enzString,enzInString,0,strlen(enzInString),strlen(enzInString),0); + else + strcpy(enzString,enzInString); + + + num_bases = getNBases(seq,EdBases); + theSeq = (char *)calloc(num_bases,sizeof(char)); + + j = 0; + if (seq->bottom) { + for (i = num_bases-1; i >= 0; i--){ + theSeq[i] = getBase(seq, EdBases, j); + j++; + } + } + else { + for (i = 0; i < num_bases; i++) + theSeq[i] = getBase(seq, EdBases, i); + } + + + + /* first look for a complete match (one mismatch allowed), then + gradually take nucleotides off the 5 prime end and continue + looking for a match */ + /* + for (i = 0; i < 3; i++) { + jj=0; + for (kk=i; enzString[kk]; kk++) enztemp[jj++]=enzString[kk]; + enztemp[jj]='\0'; + num_matches=string_match(enztemp,strlen(enztemp),theSeq,num_bases,2,indices); + if (num_matches > 0) + if (indices[0] < maxStartPos) { + found = 1; + break; + } + } + */ + + /* look for first occurrence of enzString; + just look a match with at most i mismatches, starting + with 0 mismatches down to two*/ + for (i=0; i<3; i++) { + num_matches=string_match(enzString,strlen(enzString),theSeq,num_bases,i,indices); + if (num_matches > 0) + if (indices[0] < maxStartPos) { + found = 1; + break; + } + } + + free(theSeq); + + if (found) return(indices[0] + strlen(enzString)); + else return(0); + +} + + +int findRightCutoff(Seq seq) +{ + /* give the %age cutoff a default but let it be user + specifiable on the command line ? */ + /* + * ways to look for ends of sequence + * 1. runs of nucleotides or dinucleotides + * -- but rick says there are lots of runs of + * A's and T's in what they're sequencing + * 2. percentage of N's + * 3. automatically drop down to baseNum 600 to even start + * looking for a cutoff + */ + + int num_bases; + char *theSeq; + int i,j; + int rightCutoff; + + num_bases = getNBases(seq,EdBases); + theSeq = (char *)calloc(num_bases,sizeof(char)); + + j = 0; + if (seq->bottom) { + for (i = num_bases-1; i >= 0; i--){ + theSeq[i] = getBase(seq, EdBases, j); + j++; + } + } + else { + for (i = 0; i < num_bases; i++) + theSeq[i] = getBase(seq, EdBases, i); + } + + rightCutoff = findPercntAmbig(theSeq,num_bases); + + /* added so that the left and right cutoffs do not overlap */ + if (rightCutoff > num_bases - seq->leftCutoff) rightCutoff=num_bases - seq->leftCutoff; + + free(theSeq); + return(rightCutoff); +} + + +int findPercntAmbig(char *theSeq, int num_bases) + +{ + int i,j; + int isN[256]; + int totalN = 0; + int numN; + int nucWindow; + + numN = 2; + nucWindow = 5; + + for (i = 0; i <= 256; i++) isN[i]=0; + isN['n']=1; + isN['N']=1; + isN['-']=1; + + + + /* + * start at base num 200 and look for numN Ns within + * a window of nucWindow nucleotides, once you find that + * second N send back the indices of that second + * N as the cutoff line + */ + + for (i = 200; i < num_bases; i++) { + totalN = 0; + for (j = 0; j < nucWindow; j++) { + if (isN[theSeq[i+j]]) totalN++; + if (totalN == numN) return(num_bases - (i+j)); + } + } + return(0); + +} + + diff --git a/src/ted/seqIOEdit.h b/src/ted/seqIOEdit.h new file mode 100644 index 0000000..9442d60 --- /dev/null +++ b/src/ted/seqIOEdit.h @@ -0,0 +1,115 @@ +#ifndef _seqIOEdit_h +#define _seqIOEdit_h + +/* + Title: seqIOEdit + + File: seqIOEdit.h + Purpose: IO of edited sequences + Last update: Tue Jan 15 1991 + + Change log :- + 15.01.91 SD + Removed parts required for complementation and put them in opp.h +*/ + +/* ---- Imports ---- */ + +#include "seq.h" /* IMPORT: Seq */ +#include /* IMPORT: fopen, fclose, fseek, ftell, fgetc */ +#include /* IMPORT: isprint */ + + +#include /* IMPORT: Boolean */ + +/* ---- Private ---- */ + +/* ---- Exports ---- */ + +extern Boolean writeEdSeq(Seq seq, char *fn); +/* + Write the clipped, edited part of the sequence `seq' + into file `fn'. The result indicates success. + + (*==fn in the following description) + + addition by lfw, 10/24/90, + + 1. writes *.out as text file without printing bases + before left and right cutoff, and without + printing base numbers (in this program) + + 2. write *.1 as text if seq.1 does not exist + write *.2 as text if seq.1 does exist + write *.3 as text if seq.2 does exist + ... + write *.n as text if seq.(n-1) does exist + ( in writeEdSeq in seqIOEdit.c ) + + +*/ + +extern Boolean readEdSeq(Seq seq, String fn, int dotnum); +/*reads in the most recent fn.seq.n file. This + file should be of the form: + basePos;edBasePos;edBase + returns True if it read in the sequence from + an editted file, and False if there was no + editted file or if there was a problem + + dotnum == -1 if the user did not specify a + version number of the sequence to read in, + m if they want version m read in. */ + + + +extern Boolean processEdSeqFile(Seq seq, String fn); + +/* processes a char vector containing the Editted + sequence in the following format: + + NedBases*NorigBases*leftCutoff*rightCutoff*negative + components of edits array in the form (position, + negative number)*non NULL componenets edBase array* + non NULL components edBasePos array* + + each division ends in an H. The end of the + edits array is signaled by a -1 -1 entry. Within + each division entries are separated by spaces + + sticks the information it finds into the seq array + + returns false if there was a problem with the file format */ + +extern Boolean isDotSeq(char *fn); + +/* checks to see if the filename already has the .seq + suffix or not */ + + +extern int isDotNum(char *fn); +/* checks if there is a .1 or .2 or .m on the inputfilename. +If there is, then returns that num. If not returns -1. + */ + +extern void stripDotNum(char *fn); +/* if there is a .num (indicating that the user has input + an edited version of the sequence they wish to have + read in) on the end of fn, strip it off */ + +extern int findLeftCutoff(Seq seq, char *enzString); +/* if the sequence has not previously been edited, this +subroutine is used for auto-clipping */ + + +extern int findRightCutoff(Seq seq); +/* if the sequence has not previously been edited, this +subroutine is used for auto-clipping */ + + +#endif /*_seqIOEdit_h*/ + + + + + diff --git a/src/ted/seqIOPlain.c b/src/ted/seqIOPlain.c new file mode 100644 index 0000000..e1b8c12 --- /dev/null +++ b/src/ted/seqIOPlain.c @@ -0,0 +1,120 @@ +/* + Title: seqIOPlain + + File: seqIOPlain.c + Purpose: IO of plain sequences + Last update: Tuesday Jan 15 1991 + + Change log: + + 28.11.90 SD put undesirables under STLOUIS compilation flag + 15.01.91 SD new include file (opp.h) + 17.09.91 LFW changed STLOUIS compilation flag to SAVE_EDITS + and AUTO_CLIP + */ + + +/* + This module should be regarded as part of `seq' since it is + privy to the internal structure of `Seq'. + + Any references to the writing or reading of edited sequences, + or to the bottom strand were added by lfw + */ + + + + +/* ---- Imports ---- */ + +#include "seqIOPlain.h" +#include "seq.h" /* IMPORT: Seq, BasesOnly, NULLSeq, + newSeq, freeSeq */ +#include "seqIOEdit.h" /* IMPORT: readEdSeq, writeEdSeq */ + +#include /* IMPORT: fopen, fclose, fseek, ftell, fgetc */ +#include /* IMPORT: isprint */ + + + + +/* ---- Constants ---- */ + +#define BasesPerLine 50 /* For output formatting */ + + + + +/* ---- Exports ---- */ + + + + +Seq readSeqPlain(char *fn) +/* + Read the plain format sequence with name `fn' into `seq'. + All printing characters (as defined by ANSII C `isprint') + are accepted, but `N's are translated to `-'s. + A NULLSeq result indicates failure. + */ +{ Seq seq = NULLSeq; + FILE *fp; + off_t fileLen; + int ch; + int i; + int wait_for_eol=0; /* used for looking for end of lines in the header */ + + if ((fp = fopen(fn, "r")) == NULL) return(NULLSeq); + + /* + Find the length of the file. + Use this as an overestimate of the length of the sequence. + */ + fseek(fp, (off_t) 0, 2); + if ((fileLen = ftell(fp)) > MaxInt2) + { fclose(fp); + return(NULLSeq); + } + fseek(fp, (off_t) 0, 0); + + /* Allocate the sequence */ + if ((seq = newSeq(BasesOnly, 0, fileLen)) == NULLSeq) + { fclose(fp); + return(NULLSeq); + } + seq->mode = BasesOnly; + seq->format = PlainFormat; + seq->dirty = False; + seq->bottom = False; + + /* Read in the bases */ + + seq->NorigBases = 0; + while ((ch = fgetc(fp)) != EOF) { + if (ch==';' || wait_for_eol) { + /* if you find a semicolon assume that line is a comment + and stop reading sequence until you have reached the + end of line */ + wait_for_eol=1; + if (ch=='\n') wait_for_eol=0; + } + else if (isprint(ch)) + { seq->base[seq->NorigBases] = (ch=='N') ? '-' : ch; + seq->NorigBases++; + } + } + + + seq->NedBases = seq->NorigBases; + (void) fclose(fp); + + + /* + Build a dummy basePos table such that the position of the + base is equal to its place in the sequence * 10. + */ + seq->NPoints = (seq->NorigBases)*10; + for (i=0; i < seq->NorigBases; i++) seq->basePos[i] = i*10; + + return(seq); +} diff --git a/src/ted/seqIOPlain.h b/src/ted/seqIOPlain.h new file mode 100644 index 0000000..feb513e --- /dev/null +++ b/src/ted/seqIOPlain.h @@ -0,0 +1,45 @@ +#ifndef _seqIOPlain_h +#define _seqIOPlain_h + + +/* + Title: seqIOPlain + + File: seqIOPlain.h + Purpose: IO of plain sequences + Last update: Mon May 28 1990 +*/ + + + + +/* ---- Imports ---- */ + + +#include "seq.h" /* IMPORT: Seq */ + +#include /* IMPORT: Boolean */ + + + + +/* ---- Exports ---- */ + + +extern Seq readSeqPlain(char *fn); +/* + Read the plain format sequence with name `fn' into `seq'. + All printing characters (as defined by ANSI C `isprint') + are accepted, but `N's are translated to `-'s. + A NULLSeq result indicates failure. + + enzString is used to automatically determine the left + cutoff for a given sequence -- only determined if this + is the first time this sequence has been read in. If + the sequence has already been edited, the program uses + the previous settings for left and right cutoffs + +*/ + + +#endif /*_seqIOPlain_h*/ diff --git a/src/ted/seqIOSCF.c b/src/ted/seqIOSCF.c new file mode 100644 index 0000000..cee7d13 --- /dev/null +++ b/src/ted/seqIOSCF.c @@ -0,0 +1,286 @@ +/* + Title: seqIOSCF + + File: seqIOSCF.c + Purpose: IO of Standard Chromatogram Format sequences + Last update: Tue Nov 10 1992 + + Change log: + 4 Feb 1992, Now draft proposal version 2 + 20 Feb 1992, Grab info from comment lines + 19 Aug 1992, If SCF file has clip information, don't clip automatically + 10 Nov 1992 SCF comments now stored in seq data structure + +*/ + + + + + +/* ---- Imports ---- */ + +#include "scf.h" /* SCF structures */ +#include "seq.h" /* IMPORT: Seq, BasesAndTraces, NULLSeq, + newSeq, freeSeq */ +#include "seqIOEdit.h" /* IMPORT: writeEdSeq, + readEdSEq */ + +#include +#include /* IMPORT: fopen, fclose, fseek, ftell, fgetc, + EOF */ +#include +#include "mach-io.h" + + +/* ---- Internal functions ---- */ + + +static int read_scf_header(FILE *fp, Header *h) +{ + int i; + + if (be_read_int_4(fp,&h->magic_number)==False) return (False); + if (be_read_int_4(fp,&h->samples)==False) return (False); + if (be_read_int_4(fp,&h->samples_offset)==False) return (False); + if (be_read_int_4(fp,&h->bases)==False) return (False); + if (be_read_int_4(fp,&h->bases_left_clip)==False) return (False); + if (be_read_int_4(fp,&h->bases_right_clip)==False) return (False); + if (be_read_int_4(fp,&h->bases_offset)==False) return (False); + if (be_read_int_4(fp,&h->comments_size)==False) return (False); + if (be_read_int_4(fp,&h->comments_offset)==False) return (False); + if (fread(&h->version[0],sizeof(h->version),1,fp)!=1) return (False); + if (be_read_int_4(fp,&h->sample_size)==False) return (False); + if (be_read_int_4(fp,&h->code_set)==False) return (False); + for (i=0;i<20;i++) + if (be_read_int_4(fp,&h->spare[i])==False) return (False); + + return (True); +} + + +static int read_scf_sample1(FILE *fp, Samples1 *s) +{ + if (be_read_int_1(fp,&s->sample_A)==False) return (False); + if (be_read_int_1(fp,&s->sample_C)==False) return (False); + if (be_read_int_1(fp,&s->sample_G)==False) return (False); + if (be_read_int_1(fp,&s->sample_T)==False) return (False); + + return (True); +} + + +static int read_scf_sample2(FILE *fp, Samples2 *s) +{ + if (be_read_int_2(fp,&s->sample_A)==False) return (False); + if (be_read_int_2(fp,&s->sample_C)==False) return (False); + if (be_read_int_2(fp,&s->sample_G)==False) return (False); + if (be_read_int_2(fp,&s->sample_T)==False) return (False); + + return (True); +} + + + + + +static int read_scf_base(FILE *fp, Bases *b) +{ + if (be_read_int_4(fp,&b->peak_index)==False) return (False); + if (be_read_int_1(fp,&b->prob_A)==False) return (False); + if (be_read_int_1(fp,&b->prob_C)==False) return (False); + if (be_read_int_1(fp,&b->prob_G)==False) return (False); + if (be_read_int_1(fp,&b->prob_T)==False) return (False); + if (be_read_int_1(fp,(byte *)&b->base)==False) return (False); + if (be_read_int_1(fp,&b->spare[0])==False) return (False); + if (be_read_int_1(fp,&b->spare[1])==False) return (False); + if (be_read_int_1(fp,&b->spare[2])==False) return (False); + + return (True); +} + + + +static float parse_version(char version[]) +/* + * + */ +{ + char v[5]; + strncpy(v,version,4);v[4]='\0'; + if (strspn(v,"0123456789. ")!=4) return 0.0; + return atof(v); + +} + + + +/* ---- Exports ---- */ + +int is_SCF(char *fn) +/* + * Check to see if file with name `fn' is in SCF format + * + * Returns: + * 1 - is SCF format + * 0 - not SCF format + */ +{ + FILE *fp; + uint_4 magic; + int ok; + + if ( (fp=fopen(fn,"rb")) == NULL) { + ok = 0; + } else { + if ( be_read_int_4(fp, &magic) != 1 ) { + ok = 0; + } else { + ok = (magic==SCF_MAGIC); + } + fclose(fp); + } + + return ok; +} + + + + + + + + +Seq readSeqSCF(char *fn) +/* + * Read the SCF format sequence with name `fn' into `seq'. + * If bottom is True, we read the sequence in in reverse and + * complemented A NULL result indicates failure. + */ +{ Seq seq = NULLSeq; + FILE *fp; + Header header; + int i; + + /* Open for: reading, binary */ + if ((fp = fopen(fn, "rb")) == NULL) return(NULLSeq); + + /* Read header */ + if (fseek(fp, (off_t)0, 0) != 0) { + fclose(fp); + return(NULLSeq); + } + if (read_scf_header(fp,&header)==False) { + fclose(fp); + return(NULLSeq); + } + + /* Allocate the sequence */ + if ((seq = newSeq(BasesAndTraces, header.samples, header.bases)) == NULLSeq) + { fclose(fp); + return(NULLSeq); + } + + + seq->mode = BasesAndTraces; + seq->format = SCFFormat; + seq->dirty = False; + seq->maxTraceVal = 0; + seq->NPoints = header.samples; + seq->NorigBases = header.bases; + seq->NedBases = header.bases; + seq->leftCutoff = header.bases_left_clip; + seq->rightCutoff = header.bases_right_clip; + seq->bottom = False; + + /* set precision if necessary */ + if (parse_version(header.version)NPoints;i++) { + switch ( header.sample_size ) { + case 2: + { + Samples2 sample; + if (read_scf_sample2(fp,&sample)==False) { + fclose(fp); + return(NULLSeq); + } + seq->traceA[i] = sample.sample_A; + seq->traceC[i] = sample.sample_C; + seq->traceG[i] = sample.sample_G; + seq->traceT[i] = sample.sample_T; + break; + } + default: + { + Samples1 sample; + if (read_scf_sample1(fp,&sample)==False) { + fclose(fp); + return(NULLSeq); + } + seq->traceA[i] = sample.sample_A; + seq->traceC[i] = sample.sample_C; + seq->traceG[i] = sample.sample_G; + seq->traceT[i] = sample.sample_T; + break; + } + } + if (seq->maxTraceVal < seq->traceA[i]) seq->maxTraceVal = seq->traceA[i]; + if (seq->maxTraceVal < seq->traceC[i]) seq->maxTraceVal = seq->traceC[i]; + if (seq->maxTraceVal < seq->traceG[i]) seq->maxTraceVal = seq->traceG[i]; + if (seq->maxTraceVal < seq->traceT[i]) seq->maxTraceVal = seq->traceT[i]; + } + + + /* Read bases */ + if (fseek(fp, (off_t)(header.bases_offset), 0) != 0) { + fclose(fp); + return(NULLSeq); + } + for(i=0;iNorigBases;i++) { + Bases base; + if (read_scf_base(fp,&base)==False) { + fclose(fp); + return(NULLSeq); + } + seq->base[i] = base.base; + seq->basePos[i] = base.peak_index; + } + + + /* Read selected comment information */ + { + char *comments; + + comments = (char *) malloc(header.comments_size+1); + + if (fseek(fp,(off_t)(header.comments_offset), 0) != 0) { + free(comments); + fclose(fp); + return(NULLSeq); + } + if (fread(comments,header.comments_size,1,fp)==0) { + free(comments); + fclose(fp); + return(NULLSeq); + } + comments[header.comments_size] = '\0'; + + seq->info = comments; + + } + + + + /* Finished with the file */ + fclose(fp); + + return(seq); +} diff --git a/src/ted/seqIOSCF.h b/src/ted/seqIOSCF.h new file mode 100644 index 0000000..88c2e20 --- /dev/null +++ b/src/ted/seqIOSCF.h @@ -0,0 +1,50 @@ +#ifndef _seqIOSCF_h +#define _seqIOSCF_h + + +/* + Title: seqIOSCF + + File: seqIOSCF.h + Purpose: IO of ABI sequences + Last update: Tues Dec 11 1990 +*/ + + + + +/* ---- Imports ---- */ + + +#include "seq.h" /* IMPORT: Seq */ + +#include /* IMPORT: Boolean */ + + + + +/* ---- Exports ---- */ + +extern Seq readSeqSCF(char *fn); +/* + Read the SCF format sequence with name `fn' into `seq'. + A NULLSeq result indicates failure. + + enzString is the enzyme string that is to be used for + searching for the left cutoff of sequence +*/ + + + + + +extern int is_SCF(char *fn); +/* + Check to see if file with name `fn' is in SCF format + + Returns: + 1 - is SCF format + 0 - not SCF format +*/ + +#endif /*_seqIOSCF_h*/ diff --git a/src/ted/seqOutput.c b/src/ted/seqOutput.c new file mode 100644 index 0000000..931c7b2 --- /dev/null +++ b/src/ted/seqOutput.c @@ -0,0 +1,230 @@ +/* + Title: seqOutput + + File: seqOutput.c + Purpose: Output of sequences + Last update: Monday April 8 1991 + Change log: + 27/11/90 SD writeSeqABI() outputs header to sequence file: + format: ;{noOfBases}{leftCutOff}{basesWritten}{type}{tra +cefile} + eg: ; 867 45 383ABI a09b7.s1RES + 28.11.90 SD put undesirables under STLOUIS compilation flag + 11.12.90 SD new static function tail to find file name in path name + 15.01.91 SD new include file (opp.h) + 08.04.91 SD header information now written in write_header() + 17.09.91 LFW changed STLOUIS flag to SAVE_EDITS to more accurately + reflect the flag + 07.10.91 SD Removed width of file field in write_header() + 26.11.91 SD Added SCF format on output + +*/ + + +/* + This module should be regarded as part of `seq' since it is + privy to the internal structure of `Seq'. + +*/ + + + + +/* ---- Imports ---- */ + +#include "seqIOEdit.h" +#include "opp.h" /* IMPORT: oppInitialise */ +#include "seq.h" /* IMPORT: Seq, BasesAndTraces, NULLSeq, + newSeq, freeSeq */ +#include +#include /* IMPORT: fopen, fclose, fseek, ftell, fgetc, + EOF */ +/*#include */ + + + + +/* ---- Constants ---- */ + +#define BasesPerLine 50 /* For output formatting */ + +/* ---- Private Functions ---- */ +static char *tail (char *pathname) +{ + char *a; + if ((a = (char *) strrchr(pathname,'/'))==NULL) + a = pathname; + else + a++; + return a; +} + +static void write_header(Seq seq, char *seqName, FILE *fp) +/* +Write out a header with the sequence. +The header consists of a line describing the cut off sequence, plus the +actual discarded sequences. The format is: + + ;header description + ;right cutoff sequence (possibly several lines) + +*/ +{ + int baseNum,lineLen; + + + if (seq->bottom) { + /* this is a complemented sequence */ + fprintf (fp,";%6d%6d%6d%-4s%s\n", + seq->NedBases, + seq->rightCutoff, + seq->NedBases - seq->rightCutoff - seq->leftCutoff, + (seq->format==ABIFormat)?"ABI": + (seq->format==ALFFormat)?"ALF": + (seq->format==PlainFormat)?"PLN": + (seq->format==SCFFormat)?"SCF": + " ", + tail(seqName)); + + opp['N']='-'; + + /* output left cut off */ + lineLen = 0; + for (baseNum=seq->NedBases-1; + baseNum>=seq->NedBases-seq->rightCutoff; + baseNum--) { + if (! lineLen) fprintf(fp, ";<"); + fputc(opp[getBase(seq, EdBases, baseNum)], fp); + if (++lineLen == BasesPerLine) { + fputc('\n', fp); + lineLen = 0; + } + } + if (lineLen) fputc('\n', fp); + + /* output right cut off */ + lineLen = 0; + for (baseNum=seq->leftCutoff-1; + baseNum>=0; + baseNum--) { + if (! lineLen) fprintf(fp, ";>"); + fputc(opp[getBase(seq, EdBases, baseNum)], fp); + if (++lineLen == BasesPerLine) { + fputc('\n', fp); + lineLen = 0; + } + } + if (lineLen) fputc('\n', fp); + + + } else { + /* this is a sequence in its normal orientation */ + fprintf (fp,";%6d%6d%6d%-4s%s\n", + seq->NedBases, + seq->leftCutoff, + seq->NedBases - seq->rightCutoff - seq->leftCutoff, + (seq->format==ABIFormat)?"ABI": + (seq->format==ALFFormat)?"ALF": + (seq->format==PlainFormat)?"PLN": + (seq->format==SCFFormat)?"SCF": + " ", + tail(seqName)); + + opp['N']='-'; + + /* output left cut off */ + lineLen = 0; + for (baseNum=0; + baseNumleftCutoff; + baseNum++) { + if (! lineLen) fprintf(fp, ";<"); + fputc(getBase(seq, EdBases, baseNum), fp); + if (++lineLen == BasesPerLine) { + fputc('\n', fp); + lineLen = 0; + } + } + if (lineLen) fputc('\n', fp); + + /* output right cut off */ + lineLen = 0; + for (baseNum=seq->NedBases-seq->rightCutoff; + baseNumNedBases; + baseNum++) { + if (! lineLen) fprintf(fp, ";>"); + fputc(getBase(seq, EdBases, baseNum), fp); + if (++lineLen == BasesPerLine) { + fputc('\n', fp); + lineLen = 0; + } + } + if (lineLen) fputc('\n', fp); + + } + +} + + + + +Boolean writeSeq(Seq seq, char *fn, char *seqName, Boolean includeHeader) +/* + Write the clipped, edited part of the ABI format sequence `seq' + into file `fn'. The result indicates success. + Currently, this just writes the bases out as text. +*/ + +{ FILE *fp; + int baseNum, lineLen; + + /* initialize the complement array */ + oppInitialize(); + + /* Open for writing, text */ + if ((fp=fopen(fn, "w")) == NULL) return(False); + + lineLen = 0; + + /* write header */ + if (includeHeader) write_header(seq,seqName,fp); + + if (seq->bottom) { /* this if seq-> bottom section + added by lfw */ + opp['N']='-'; + + for (baseNum=(seq->NedBases-seq->rightCutoff)-1; + baseNum>=seq->leftCutoff; + baseNum--) + { fputc(opp[getBase(seq, EdBases, baseNum)], fp); + if (++lineLen == BasesPerLine) + { fputc('\n', fp); + lineLen = 0; + } + } + } + else { + for (baseNum=seq->leftCutoff; + baseNumNedBases-seq->rightCutoff; + baseNum++) + { fputc(getBase(seq, EdBases, baseNum), fp); + if (++lineLen == BasesPerLine) + { fputc('\n', fp); + lineLen = 0; + } + } + } + + if (lineLen != 0) fputc('\n', fp); + + (void) fclose(fp); + +#ifdef SAVE_EDITS + if (writeEdSeq(seq,seqName)) return(True); + else return(False); +#else + return(True); +#endif +} + + diff --git a/src/ted/seqOutput.h b/src/ted/seqOutput.h new file mode 100644 index 0000000..79f3d18 --- /dev/null +++ b/src/ted/seqOutput.h @@ -0,0 +1,18 @@ +#ifndef _seqOutput_h +#define _seqOutput_h +/* + Title: seqOutput + + File: seqOutput.h + Purpose: Output of sequences + +*/ + +extern Boolean writeSeq(Seq seq, char *fn, char *seqName,Boolean includeHeader); +/* + Write the clipped, edited part of the ABI format sequence `seq' + into file `fn'. `seqName' is for sequence header information. + The result indicates success. + Currently, this just writes the bases out as text. +*/ +#endif /*_seqOutput_h*/ diff --git a/src/ted/seqRead.c b/src/ted/seqRead.c new file mode 100644 index 0000000..3862da2 --- /dev/null +++ b/src/ted/seqRead.c @@ -0,0 +1,131 @@ +#include +#include "seq.h" +#include "opp.h" +#include "seqIOABI.h" /* IMPORT: readSeqABI */ +#include "seqIOALF.h" /* IMPORT: readSeqALF */ +#include "seqIOSCF.h" /* IMPORT: readSeqSCF */ +#include "seqIOPlain.h" /* IMPORT: readSeqPlain */ +#include "traceDisplay.h" /* IMPORT: setScaleFactor */ + + + +Seq readSeq(char *fn, int bottom, char *enzString, char *format) +{ + Seq seq; +#ifdef SAVE_EDITS + int dotnum=-1; +#endif /*SAVE_EDITS*/ + FILE *fp; + /* the number of the edited sequence the + user wishes to read in. It's a -1 if the + user wishes to read the most recent sequence */ + int found_edited_sequence=0; /* indicates if there was a correct .n file to + read when the program went to read + the edited sequence file */ + + + + if ( (fp=fopen(fn,"r")) == NULL) { + + /* + * 920917 this chunk of code was moved by lfw + * so that ted first tries to open up the filename + * as given by the user...if that is not a trace + * file then it goes ahead and strips off the dotnum + * and tries to open that + */ + +#ifdef SAVE_EDITS + /* + * check to see if the file the user asked to read in is + * a .1 or .2 or .m; if it is they must have wanted to + * read in a specific edited sequence + */ + + dotnum = isDotNum(fn); + if (dotnum != -1) stripDotNum(fn); +#else + return(NULLSeq); +#endif + + } else { + fclose(fp); + } + + /* + * read in the sequence + */ + if (strcmp(format, "plainFmt") == 0) + seq = readSeqPlain(fn); + else if (strcmp(format, "abiFmt") == 0) + seq = readSeqABI(fn); + else if (strcmp(format, "alfFmt") == 0) + seq = readSeqALF(fn); + else if (strcmp(format, "scfFmt") == 0) + seq = readSeqSCF(fn); + + /* + * further processing + */ + if (seq != NULLSeq) { + + int i; + + /* + + Go ahead and Initialise the edited sequence + with orig bases, then go to read the Edited + sequence to override those bases which have been + changed + + */ + + + /* All ed bases are the same are orig bases */ + for (i=0;iNorigBases;i++) seq->edits[i] = i; + + /* The table of edits has all its entries free */ + for (i=0;iedBasePos[i] = NULLPoint; + + + + + found_edited_sequence=0; + +#ifdef SAVE_EDITS + found_edited_sequence=readEdSeq(seq,fn,dotnum); +#endif + +#ifdef AUTO_CLIP + if (!found_edited_sequence && + seq->leftCutoff == 0 && + seq->rightCutoff == 0) { + + + if (seq->bottom) { + seq->rightCutoff = findLeftCutoff(seq,enzString); + seq->leftCutoff = findRightCutoff(seq); + } + else { + seq->leftCutoff = findLeftCutoff(seq,enzString); + seq->rightCutoff = findRightCutoff(seq); + } + + } +#endif + } + + /* initialize the complement array */ + oppInitialize(); + + if (bottom) complement_seq(seq); + + /* set default scale factor */ + setScaleFactor(1.0); + return seq; + +} + + + + diff --git a/src/ted/seqRead.h b/src/ted/seqRead.h new file mode 100644 index 0000000..ff5d4a0 --- /dev/null +++ b/src/ted/seqRead.h @@ -0,0 +1,6 @@ +#ifndef _SEQREAD_H_ +#define _SEQREAD_H_ + +extern Seq readSeq(char *fn, int bottom, char *enzString, char *format); + +#endif /*_SEQREAD_H_*/ diff --git a/src/ted/ted.c b/src/ted/ted.c new file mode 100644 index 0000000..fcb3f9a --- /dev/null +++ b/src/ted/ted.c @@ -0,0 +1,716 @@ +/* + Title: ted + + File: ted.c + Purpose: Main module + Last update: 23 June 1992 + + Change log: + + 28.11.90 SD put undesirables under STLOUIS compilation flag + 22.07.91 LFW put in -enzyme as a command line option for STLOUIS + 30.07.91 SD fixed bug in MRC switch + 17.09.91 LFW changed MRC switch to !AUTO_CLIP and STLOUIS flag + to DEF_OUT to more accurately reflect the use of the flags + 26.11.91 SD Added SCF format + 23-Jun-92 SD Some ugly code in DEF_OUT option +*/ + + +/* + This module contains the C language entry point `main' and + initialisation for the X system. +*/ + + + + +/* ---- Includes ---- */ + +#include "dialogues.h" /* IMPORT: inputSeq, outputSeq, quitApplication */ +#include "display.h" /* IMPORT: createDisplay */ +#include "help.h" /* IMPORT: createHelpShell, help */ +#include "seq.h" /* IMPORT: Seq, NULLBaseNum */ +#include "seqIOEdit.h" /* IMPORT: isDotNum, stripDotNum */ + +#include /* IMPORT: stderr, fprintf */ +#include "mystdlib.h" /* IMPORT: exit */ + +#include +#include +#include +#include +#include + + +/* ---- Static variables ---- */ + + +static String fallback_resources[] = +{ /* The application */ + "Xted.minWidth: 550", + "Xted.minHeight: 250", + + "Xted*magnif: 90", + + /* Labels have no borders and are not resized */ + "Xted*Label.borderWidth: 0", + "Xted*Label.top: ChainTop", + "Xted*Label.bottom: ChainTop", + "Xted*Label.left: ChainLeft", + "Xted*Label.right: ChainLeft", + + /* Buttons are not resized */ + "Xted*Command.top: ChainTop", + "Xted*Command.bottom: ChainTop", + "Xted*Command.left: ChainLeft", + "Xted*Command.right: ChainLeft", + + /* All the toggles are one-of-many */ + "Xted*Toggle.Translations: #override \\n\ + : highlight(Always) \\n\ + : unhighlight() \\n\ + ,: set() notify()", + + "Xted.mainForm.title.label: Trace editor", + "Xted.mainForm.version.label: \ + June 1991", + "Xted.mainForm.seqNamePrompt.label: Input:", + "Xted.mainForm.seqNamePrompt.height: 13", + "Xted.mainForm.seqName.label: NONE", + "Xted.mainForm.seqName.resizable: True", + "Xted.mainForm.seqName.height: 13", + "Xted.mainForm.NorigBasesPrompt.label: Bases:", + "Xted.mainForm.NorigBasesPrompt.height: 13", + "Xted.mainForm.NorigBases.label: ", + "Xted.mainForm.NorigBases.resizable: True", + "Xted.mainForm.strand.label: Strand: Top", + "Xted.mainForm.strand.resizable: True", + + + /* Display forms have no border */ + "Xted.mainForm.Form.borderWidth: 0", + + /* The edit mode toggles */ + "Xted.mainForm.Toggle.top: ChainTop", + "Xted.mainForm.Toggle.bottom: ChainTop", + "Xted.mainForm.Toggle.left: ChainLeft", + "Xted.mainForm.Toggle.right: ChainLeft", + "Xted.mainForm.edmodelab.label: Mode:", + "Xted.mainForm.adjustL.label: Adj left cut", + "Xted.mainForm.edSeq.label: Edit seq", + "Xted.mainForm.adjustR.label: Adj right cut", + "Xted.mainForm.edSeq.state: True", /* Default toggle */ + "Xted.mainForm.adjustL.height: 13", + "Xted.mainForm.edSeq.height: 13", + "Xted.mainForm.adjustR.height: 13", + + /* The magnification slider */ + "Xted.mainForm.maglab.top: ChainTop", + "Xted.mainForm.maglab.bottom: ChainTop", + "Xted.mainForm.maglab.left: ChainLeft", + "Xted.mainForm.maglab.right: ChainLeft", + "Xted.mainForm.maglab.label: Mag:", + "Xted.mainForm.maglab.height: 13", + + "Xted.mainForm.magscr.top: ChainTop", + "Xted.mainForm.magscr.bottom: ChainTop", + "Xted.mainForm.magscr.left: ChainLeft", + "Xted.mainForm.magscr.right: ChainLeft", + "Xted.mainForm.magscr.orientation: horizontal", + "Xted.mainForm.magscr.length: 100", + "Xted.mainForm.magscr.height: 13", + + "Xted.mainForm.scaleDown.label: Scale down", + "Xted.mainForm.scaleDown.height: 13", + "Xted.mainForm.scaleUp.label: Scale up", + "Xted.mainForm.scaleUp.height: 13", + "Xted.mainForm.switch.label: Switch", + "Xted.mainForm.switch.height: 13", + + /* The main display: a viewport containing a form containing graphs */ + "Xted.mainForm.viewport.top: ChainTop", + "Xted.mainForm.viewport.bottom: ChainBottom", + "Xted.mainForm.viewport.left: ChainLeft", + "Xted.mainForm.viewport.right: ChainRight", + "Xted.mainForm.viewport.allowHoriz: True", + "Xted.mainForm.viewport.forcebars: True", + "Xted.mainForm.viewport.width: 700", + "Xted.mainForm.viewport.vpForm.Graph.resizable: True", + "Xted.mainForm.viewport.vpForm.Graph.top: ChainTop", + "Xted.mainForm.viewport.vpForm.Graph.bottom: ChainTop", + "Xted.mainForm.viewport.vpForm.Graph.left: ChainLeft", + "Xted.mainForm.viewport.vpForm.Graph.right: ChainRight", + "Xted.mainForm.viewport.vpForm.trace.top: ChainTop", + "Xted.mainForm.viewport.vpForm.trace.bottom: ChainBottom", + "Xted.mainForm.viewport.vpForm.Graph.font: -misc-*-bold-*15-*", + /* The text graph heights are dynamically set to accomodate the font */ + "Xted.mainForm.viewport.vpForm.trace.height: 150", + /* The graphs are dynamically initially set to fill the viewport */ + "Xted.mainForm.viewport.vpForm.Graph.dimBackground: grey", + "Xted.mainForm.viewport.vpForm.Graph.graphColour1: green", + "Xted.mainForm.viewport.vpForm.Graph.graphColour2: purple", + "Xted.mainForm.viewport.vpForm.Graph.graphColour3: black", + "Xted.mainForm.viewport.vpForm.Graph.graphColour4: red", + + /* Dummy widget - we only care about its width */ + "Xted.mainForm.dummy.top: ChainTop", + "Xted.mainForm.dummy.bottom: ChainBottom", + "Xted.mainForm.dummy.left: ChainLeft", + "Xted.mainForm.dummy.right: ChainRight", + "Xted.mainForm.dummy.width: 700", + + + /* Dialogues */ + "Xted*input.ioForm.ioPrompt.label: Input a file", + "Xted*search.ioForm.ioPrompt.label: Search for string or base number", + "Xted*output.ioForm.ioPrompt.label: Save a file", + "Xted*check.ioForm.ioPrompt.label: \ +The existing sequence has been altered.\\n\ +Do you really want to overwrite it?", + "Xted*checkwrite.ioForm.ioPrompt.label: \ +This output file already exists.\\n\ +Do you really want to overwrite it?", + "Xted*quit.ioForm.ioPrompt.label: \ +The existing sequence has been altered.\\n\ +Do you really want to quit?", + "Xted*check.ioForm..translations: #override \\n\ + Return: CRAction()", + "Xted*quit.ioForm..translations: #override \\n\ + Return: CRAction()", + + "Xted*ioForm.namePrompt.label: File name:", + "Xted*ioForm.nameVal.width: 200", + "Xted*ioForm.nameVal.translations: #override \\n\ + Return: CRAction()", + "Xted*ioForm.formatPrompt.label: File format:", + "Xted*ioForm.formatBox.borderWidth: 0", + "Xted*ioForm.formatBox.orientation: horizontal", + "Xted*ioForm.formatBox.plainFmt.label: Plain", + "Xted*ioForm.formatBox.abiFmt.label: ABI", + "Xted*ioForm.formatBox.abiFmt.state: True", /* Default toggle */ + "Xted*ioForm.formatBox.alfFmt.label: ALF", + "Xted*ioForm.formatBox.alfFmt.state: False", + "Xted*ioForm.formatBox.scfFmt.label: SCF", + "Xted*ioForm.formatBox.scfFmt.state: False", + "Xted*ioForm.formatBox.bottomFmt.label: Bottom", + "Xted*ioForm.formatBox.topFmt.label: Top", + "Xted*ioForm.formatBox.topFmt.state: True", /* Default toggle */ + /* The `default' button for the dialogue has a wider border */ + "Xted*input.ioForm.OK.borderWidth: 3", + "Xted*output.ioForm.OK.borderWidth: 3", + "Xted*check.ioForm.Cancel.borderWidth: 3", + "Xted*quit.ioForm.Cancel.borderWidth: 3", + + /* Help shell widget */ + "Xted.Help.width: 525", + "Xted.Help.height: 200", + "Xted.Help.minWidth: 525", + "Xted.Help.maxWidth: 525", + "Xted.Help.minHeight: 150", + "Xted.Help.title: ted help", + "Xted.Help.form.rmButton.label: Remove help", + "Xted.Help.form.rmButton.top: ChainTop", + "Xted.Help.form.rmButton.bottom: ChainTop", + "Xted.Help.form.rmButton.left: ChainLeft", + "Xted.Help.form.rmButton.right: ChainLeft", + "Xted.Help.form.text.top: ChainTop", + "Xted.Help.form.text.bottom: ChainBottom", + "Xted.Help.form.text.left: ChainLeft", + "Xted.Help.form.text.right: ChainRight", + + NULL, +}; + +/* + The command line arguments and how to parse them into resources. +*/ +static XrmOptionDescRec options[] = +{ + {"-PLN", "PLN", XrmoptionSepArg, NULL}, + {"-ABI", "ABI", XrmoptionSepArg, NULL}, + {"-ALF", "ALF", XrmoptionSepArg, NULL}, + {"-SCF", "SCF", XrmoptionSepArg, NULL}, + {"-output", "output", XrmoptionSepArg, NULL}, + {"-baseNum", "baseNum", XrmoptionSepArg, NULL}, + {"-mag", "mag", XrmoptionSepArg, NULL}, + {"-astring", "astring", XrmoptionSepArg, NULL}, + {"-bottom", "bottom", XrmoptionSepArg, NULL}, + {"-raw", "raw", XrmoptionSepArg, NULL}, + {"-enzyme","enzyme",XrmoptionSepArg,NULL}, + +}; + +/* + Where to put the command line arguments when we dig them + out of the resources. +*/ +typedef struct +{ String plain; + String ABI; + String ALF; + String SCF; + String output; + int baseNum; + int mag; + String astring; + int bottom; + String raw; + String enzyme; +} AppResources; + +/* + Dig the command line arguments out of resources and into + the above structure. +*/ +static XtResource tedResources[] = +{ + {"PLN", "Plain", XtRString, sizeof(String), + XtOffset(AppResources *, plain), XtRImmediate, NULL}, + {"ABI", "ABI", XtRString, sizeof(String), + XtOffset(AppResources *, ABI), XtRImmediate, NULL}, + {"ALF", "ALF", XtRString, sizeof(String), + XtOffset(AppResources *, ALF), XtRImmediate, NULL}, + {"SCF", "SCF", XtRString, sizeof(String), + XtOffset(AppResources *, SCF), XtRImmediate, NULL}, + {"output", "output", XtRString, sizeof(String), + XtOffset(AppResources *, output), XtRImmediate, NULL}, + {"baseNum", "baseNum", XtRInt, sizeof(int), + XtOffset(AppResources *, baseNum), XtRImmediate, (XtPointer) NULLBaseNum}, + {"mag", "mag", XtRInt, sizeof(int), + XtOffset(AppResources *, mag), XtRImmediate, NULL}, + {"astring", "astring", XtRString, sizeof(String), + XtOffset(AppResources *, astring), XtRImmediate, NULL}, + {"bottom", "bottom", XtRInt, sizeof(int), + XtOffset(AppResources *, bottom), XtRImmediate, NULL}, + {"raw", "raw", XtRString, sizeof(String), + XtOffset(AppResources *, raw), XtRImmediate, NULL}, + {"enzyme", "enzyme", XtRString, sizeof(String), + XtOffset(AppResources *, enzyme), XtRImmediate, NULL}, +}; + + + +typedef struct +{ int magnif; + String enz; +} AppData, *AppDataPtr; + + +#define XtNmagnif "magnif" +#define XtCMagnif "Magnif" +#define XtNenz "enz" +#define XtCEnz "Enz" + +static XtResource resources[] = { + { XtNmagnif, + XtCMagnif, + XtRInt, + sizeof(int), + XtOffset(AppDataPtr, magnif), + XtRImmediate, + (caddr_t)30 /* default is 30, although you can override that from + the app-defaults file or on the command line */ + }, + { XtNenz, + XtCEnz, + XtRString, + sizeof(String), + XtOffset(AppDataPtr, enz), + XtRImmediate, + NULL + } + }; + + +/* ---- Callbacks ---- */ + + +static void inputCallback(Widget widget, + XtPointer client_data, XtPointer call_data) +{ inputSeq(widget); +} + + +static void searchCallback(Widget widget, + XtPointer client_data, XtPointer call_data) +{ inputSearchString(widget); +} + + + +static void outputCallback(Widget widget, + XtPointer client_data, XtPointer call_data) +/* + client_data contains the default (NULL) or command line specified + output file name to be used. +*/ +{ + +/* this next statement was added by lfw 10/16/90, to assure that + a default output filename of inputfilename.seq will be assigned.*/ + + if (client_data!=NULL) + sprintf(o_fn,"%s",client_data); + +#ifdef DEF_OUT + if (isDotNum(o_fn) != -1) stripDotNum(o_fn); + + if (!isDotSeq(o_fn)) + strcat(o_fn,".seq"); + /* + ** This is an aweful way of accomplishing something very simple + { char junk[strlen(o_fn)+5]; + strcpy(junk,o_fn); + sprintf(o_fn,"%s.seq",junk); + } + */ +#endif + + outputSeq(widget, (String) o_fn); +} + + +static void helpCallback(Widget widget, + XtPointer client_data, XtPointer call_data) +{ + help(); +} + +static void infoCallback(Widget widget, + XtPointer client_data, XtPointer call_data) +{ + information(widget); +} + + +static void quitCallback(Widget widget, + XtPointer client_data, XtPointer call_data) +{ /* Quit the application */ + quitApplication(widget); +} + + + + +/* ---- Internal functions ---- */ + + +static void usage(int argc, char **argv) +{ int i; + + if (argc != 1) + { fprintf(stderr, "%s: unknown option%s:", argv[0], (argc>2)?"s":""); + for (i=1; iCcA_YDw_-l#V?KzAU=e~SARr~Fln4^i-5@O~ND2~C z(hU+~pd!e7U3+kSYkh0I-+$kkb>H{Ip1prvd(WIZ@2YfPo~m_Ud*j_adGh2v{bZg} z{9d0YPm(9kX|Mg0=cU70C;xx_JO8s8H|DwKl1t}T{eSifURB|ma*y2k)DxASEq7m~ 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