log tnt.log; macro=; taxname+1000; taxname=; mxram 10240; nstates 32; nstates NOGAPS; /*Arguments*/ if ( argnumber != 1 ) silent -console; quote - /-----------------------------------------------\; quote - | GUOYI TNT SCRIPT 2022-2023 MIT |; quote - | You need to give your filename |; quote - | tnt*> guoyi filename; |; quote - \-----------------------------------------------/; proc/; end /*Report what will be done*/ quote - /-----------------------------------------------\; quote - | Implied weighting will be estimated. |; quote - | TBR Mult and Xmult will be performed. |; quote - | Strict consensus will be used. |; quote - | Relative bremer support, jackknifing and |; quote - | bootstrap will be shown on the con.svg. |; quote - | Apomorphic characters mapping will be shown |; quote - | on the apo.svg and saved to apo*.tre. |; quote - | TL, CI and RI will be calculated finally. |; quote - \------------------------------------------------/; /*Set K*/ procedure %1; var: actK minK maxK gVal a b Nval maxIts ; if (ntax<0) quote There is no data; proc/; end /** find the value of g **/ loop 0 nchar if (!isinfo[#1]) continue; end if (!isact[#1]) continue; end set a=maxsteps[#1]; set b=minsteps[#1]; if (('a'-'b')>'gVal') set gVal 'a'-'b'; end stop quote g value: 'gVal'; set actK 10; set minK 0; set maxK 500; /** Assume 500 as the maximum possible value of K **/ set maxIts 0; /** find the best value of K **/ loop 0 1 quote actual k value: 'actK'; set a 1-('actK'/('actK'+1)); quote 'a'; set b ('actK'/('actK'+'gVal'-1))-('actK'/('actK'+'gVal')); quote 'b'; set Nval 'a'/'b'; quote N value: 'Nval'; if (('Nval'>14.8)&&('Nval'<15.2)) endloop; end /** N is in range **/ if ('Nval'>15.2) set minK 'actK'; else set maxK 'actK'; end set a ('maxK'-'minK')/2; set actK 'minK' + 'a'; set maxIts ++; if ('maxIts'==100) endloop; end /** emergency exit **/ setloop 0; stop /** If out of emergency **/ if ('maxIts'==100) quote Iterations are not finished; quote Best K is 'actK'; proc /; end quote K value: 'actK' (N='Nval'); piwe='actK'; /*Reopen tnt*/ procedure %1; hold 5000; /*Search trees*/ xpiwe(*; mult=replic 1000 tbr hold 10; sect: slack 1000; xmult=rss hit 1000 drift 10 ratchet 10 fuse 10; piwe&; /*Export trees*/ export= trees.tre; taxname-; export= trees_no.tre; taxname=; /*Consensus tree*/ bbreak=tbr; nelsen *; /*Get Bremer/jak/boot support*/ ttags=; sub 1; hold +1000; bbreak=fill; sub 2; hold +2000; bbreak=fill; sub 3; hold +3000; bbreak=fill; sub 4; hold +4000; bbreak=fill; sub 5; hold +5000; bbreak=fill; sub 6; hold +6000; bbreak=fill; sub 7; hold +7000; bbreak=fill; sub 8; hold +8000; bbreak=fill; bsupport[; resample jak replications 1000; resample boot replications 1000; /*Export consensus tree with supports*/ ttags & con.svg thickness 7 italics fontsize 15; export < con.tre; taxname-; export - con_no.tre; /*Apomorphic characters*/ export = winclada.tre; taxname =; ttags-; ttags=; apo >0; ttags & apo.svg thickness 7 italics fontsize 15; export < apo.tre; taxname-; export - apo_no.tre; ttags-; /*Caulculate TL/CI/RI score*/ length *; report-; var-; var = 0 themin 1 themax + CIs [ ( ntrees + 1 ) ] + RIs [ ( ntrees + 1 ) ] + this ; set themin minsteps; set themax maxsteps; loop 0 ntrees progress #1 (ntrees+1) Calculating indices...; set this length[#1]; set CIs[#1] 'themin'/'this'; /*CI=1 means no homoplasy*/ set RIs[#1] ('themax'-'this')/('themax'-'themin'); /*RI=1 character fits perfetcly*/ stop progress/; report=; macfloat 3; maketable CIs Consistency index; maketable RIs Retention index; /*Report*/ quote - /----------------------------------------------\; quote - | The analysis has been finished. |; quote - | The file `tnt.log` contains |; quote - | K, TL, CI and RI |; quote - | The file `trees*.tre` contain |; quote - | trees found by mult and xmult |; quote - | The file `con*.tre` contain |; quote - | strict consensus tree |; quote - | The file `apo*.tre` contain |; quote - | tree with apomorphic character |; quote - | The file `*_no.tre` contain |; quote - | tree with `taxaname-` |; quote - | The file `con.svg` contain |; quote - | strict consensus tree with |; quote - | relative bremer, jak and boot |; quote - | support on the tree |; quote - | The file `apo.svg` contain the |; quote - | tree with apomorphy mapping |; quote - | The file `winclada.tre` can be |; quote - | converted by tnt2winclada |; quote - \----------------------------------------------/; /*Quit*/ zzz;