RGBEPP.d

@@ -175,40 +175,6 @@ void processQcTrim(string[] ARG_L, int ARG_T, string DirRaw, string DirQcTrim, s
     writeln("QcTrimming::End");
 }
 
-void processMappingDenovo(string[] ARG_L, int ARG_T, string DirQcTrim, string DirAssembly, string DirMap, string PathBowtie2, string PathSamtools){
-    // Prepare directory
-    createDir(DirMap);
-    createDir(DirMap ~ "/index");
-    string DirAssemblySca = DirAssembly ~ "/" ~ "scaffolds";
-    string DirAssemblyFas = DirAssembly ~ "/" ~ "fasta";
-    createDir(DirAssemblySca);
-    createDir(DirAssemblyFas);
-    string ReferDmnd = DirAssemblySca ~ "/" ~ "Reference.dmnd";
-    string PathBowtie2_build = PathBowtie2 ~ "-build";
-
-    foreach (string file; ARG_L) {
-	string baseName = getBaseName(file);
-	string inputM8 = DirAssemblySca ~ "/" ~ baseName ~ ".m8";
-	string inputFasta = DirAssemblySca ~ "/" ~  baseName ~ ".fasta";
-	string outputSort = DirAssemblyFas ~ "/" ~ baseName ~ ".fasta";
-	string outputIndex = DirAssemblyFas ~ "/" ~ baseName;
-        string inputFileR1 = DirQcTrim ~ "/" ~ baseName ~ "_R1.fastq.gz";
-        string inputFileR2 = DirQcTrim ~ "/" ~ baseName ~ "_R2.fastq.gz";
-	string outputBam = DirMap ~ "/" ~ baseName ~ ".bam";
-
-	string[] cmdDiamond = ["diamond", "blastx", "-d", "Reference.dmnd", "-q", inputFasta, "-o", inputM8, "--outfmt", "6", "qseqid", "sseqid", "pident", "length", "mismatch", "gapopen", "qstart", "qend", "sstart", "send", "evalue", "bitscore", "qlen", "slen", "gaps", "ppos", "qframe", "qseq"];
-	string[] cmdSortDiamond = ["sortdiamond", inputM8, outputSort];
-    	string[] cmdBuildDB = [PathBowtie2_build, "--threads", ARG_T.to!string, outputSort, outputIndex];
-        string[] cmdMap = [PathBowtie2, "-x", outputIndex, "-1", inputFileR1, "-2", inputFileR2, "-p", ARG_T.to!string];
-        string[] cmdSam2Bam = [PathSamtools, "view", "-bS", "-@", ARG_T.to!string, "-o", outputBam];
-	executeCommand(cmdDiamond);
-	executeCommand(cmdSortDiamond);
-    	executeCommand(cmdBuildDB);
-	executeCommandPipe([cmdMap, cmdSam2Bam]);
-    }
-
-}
-
 void processMapping(string[] ARG_L, string ARG_R, int ARG_T, string DirQcTrim, string DirMap, string PathBowtie2, string PathSamtools) {
     writeln("Mapping::Start");
 
@@ -266,29 +232,6 @@ void processPostMap(string[] ARG_L, int ARG_T, string DirMap, string DirBam, str
     writeln("PostMapping::End");
 }
 
-void processVarCallDenovo(string[] ARG_L, int ARG_T, string DirAssembly, string DirMap, string DirBam, string DirVcf, string PathBcftools) {
-    writeln("VarCalling::Start");
-
-    string DirAssemblyFas = DirAssembly ~ "/" ~ "fasta";
-    createDir(DirVcf);
-
-    foreach (string file; parallel(ARG_L, 1)) {
-        string baseName = getBaseName(file);
-        string inputBam = DirBam ~ "/" ~ baseName ~ ".bam";
-        string outputVcf = DirVcf ~ "/" ~ baseName ~ ".vcf.gz";
-	string referFasta = DirAssemblyFas ~ "/" ~ baseName ~ ".fasta";
-        // Variant calling using bcftools
-        string[] cmdPileup = [PathBcftools, "mpileup", "-Oz", "--threads", ARG_T.to!string, "-f", referFasta, inputBam];
-	string[] cmdVarCall = [PathBcftools, "call", "-mv", "-Oz", "--threads", ARG_T.to!string];
-	string[] cmdNorm = [PathBcftools, "norm", "--threads", ARG_T.to!string, "-f", referFasta, "-Oz"];
-	string[] cmdFilter = [PathBcftools, "filter", "--threads", ARG_T.to!string, "--IndelGap", "5", "-Oz", "-o", outputVcf];
-        executeCommandPipe([cmdPileup, cmdVarCall, cmdNorm, cmdFilter]); 
-    }
-
-    writeln("VarCalling::End");
-
-}
-
 
 void processVarCall(string[] ARG_L, string ARG_R, int ARG_T, string DirMap, string DirBam, string DirVcf, string PathBcftools) {
     writeln("VarCalling::Start");
@@ -315,23 +258,6 @@ void processVarCall(string[] ARG_L, string ARG_R, int ARG_T, string DirMap, stri
 
 }
 
-void processConSam(string[] ARG_L, string DirBam, string DirConsensus, string PathSamtools){
-    writeln("Consensus::Start");
-
-    string DirConTaxa = DirConsensus ~ "/" ~ "taxa";
-    createDir(DirConsensus);
-    createDir(DirConTaxa);
-
-    foreach (string file; parallel(ARG_L,1)) {
-	    string baseName = getBaseName(file);
-	    string inputBam = DirBam ~ "/" ~ baseName ~ ".bam";
-	    string outputFasta = DirConTaxa ~ "/" ~ baseName ~ ".fasta";
-	    string [] cmdConsen1 = [PathSamtools, "consensus", "-f", "fasta", inputBam, "-o", outputFasta];
-	    executeCommand(cmdConsen1); 
-    }
-    writeln("Consensus::End");
-}
-
 void processCon(string[] ARG_G, string[] ARG_L, string ARG_R, int ARG_T, string DirMap,  string DirVcf, string DirConsensus, string PathBcftools) {
     createDir(DirConsensus);
 
@@ -497,7 +423,6 @@ void main(string[] args) {
     string DirBam = DirHome ~ "/03_bam";
     string DirVcf = DirHome ~ "/04_vcf";
     string DirConsensus = DirHome ~ "/05_consen";
-    string DirConsensus1 = DirHome ~ "/05_consen1";
     string DirAlign = DirHome ~ "/06_macse"; 
     string DirTrim = DirHome ~ "/07_trimal"; 
 
@@ -623,8 +548,7 @@ void main(string[] args) {
 
     if (ARG_F == "all" || ARG_F == "map") {
 	if(testFiles([PathBowtie2, PathSamtools])){
-          //processMapping(ARG_L, ARG_R, ARG_T, DirQcTrim, DirMap, PathBowtie2, PathSamtools);
+          processMapping(ARG_L, ARG_R, ARG_T, DirQcTrim, DirMap, PathBowtie2, PathSamtools);
-	  processMappingDenovo(ARG_L, ARG_T, DirQcTrim, DirAssembly, DirMap, PathBowtie2, PathSamtools);
 	}
     }
 
@@ -636,16 +560,13 @@ void main(string[] args) {
 
     if (ARG_F == "all" || ARG_F == "varcall") {
 	if(testFiles([PathBcftools])){
-            //processVarCall(ARG_L, ARG_R, ARG_T, DirMap, DirBam, DirVcf, PathBcftools);
+            processVarCall(ARG_L, ARG_R, ARG_T, DirMap, DirBam, DirVcf, PathBcftools);
-	    processVarCallDenovo(ARG_L, ARG_T, DirAssembly, DirMap, DirBam, DirVcf, PathBcftools);
-
 	}
     }
 
     if (ARG_F == "all" || ARG_F == "consen") {
 	if(testFiles([PathBcftools])){
           processCon(ARG_G, ARG_L, ARG_R, ARG_T, DirMap, DirVcf, DirConsensus, PathBcftools);
-	  //processConSam(ARG_L, DirBam, DirConsensus, PathSamtools);
 	  processCombFasta(ARG_G, ARG_L, DirConsensus);
 	}
     }