diff --git a/README.md b/README.md
index 66457b1..54d05f6 100644
--- a/README.md
+++ b/README.md
@@ -135,15 +135,21 @@ grep '>' Reference.fasta | sed "s@>@@g" > genes
 
 ### sortdiamond
 
-Usage: sortdiamond diamond_output.m8 generated.fasta sseq,qstart,qend,bitscore/evalue,qseq(optional, default 1,6,7,11,17, start from 0) bitscore/evalue(optional, default bitscore)
+Usage: `sortdiamond diamond_output.m8 generated.fasta sseq,qstart,qend,bitscore/evalue,qseq(optional, default 1,6,7,11,17, start from 0) bitscore/evalue(optional, default bitscore)`
 
 Default sseq is column 2, qstart is column 8, etc.
 
 Diamond default output format (--outfmt 6) does not contain qseq, you must custom the output format under output format 6. 
 
+### delstop
+
+`delstop <fasta_aa> <fasta_nt> --delete`
+
+Delete StopCondon generated by Macse. fasta_aa and fasta_nt should be macse output files, `--delete` should be used when downstream software is tirmal
+
 ### splitfasta
 
-Usage: splitfasta sample.fasta
+Usage: `splitfasta sample.fasta`
 
 It always creates directories in the path that you run the splitfasta, and puts split fasta into the directory.